LOCUS       DAZBSA010000001       100444 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000001 DAZBSA010000000
VERSION     DAZBSA010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 100444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 100444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..100444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.001"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..523)
                     /locus_tag="ACHGMT_000001"
     CDS             complement(<1..523)
                     /locus_tag="ACHGMT_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6833783.1"
                     /translation="MSQWNTIDELRDIFIRKYTDSELSNELEKACSEEYELMRDYNGR
                     QILELLQNVDDAYGDIKSENDASNDEVKVQITYKNNILEVGNTGTSFSKDTIERLCLG
                     RASNKSSQNIGNKGTGFRSLLNDAEWVELYSGEFAIRFSEEFTKKLFEQYVSRDSDKF
                     SELIFEQKKNWKKE"
     gene            complement(646..2196)
                     /locus_tag="ACHGMT_000002"
     CDS             complement(646..2196)
                     /locus_tag="ACHGMT_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN6833784.1"
                     /translation="MAGKQVTVIPMKPRQWAAENTEEKPKLKVAAYCRVSTEMEEQAS
                     SYEAQVQHYTDYIQRNPDWELVGIFADEGISGTGTKKRDGFNRMIEACQNGEVEYIIT
                     KSISRFARNTVDCLQYIRQLKELKIAVFFEKENINTMDAKGEVLLTIMASLAQQESQS
                     LSQNTKMGVQYRFQQGQLRINHNHFLGYTKDEDGNLVIEPKEAEVIKRIFREYLEGSS
                     LQEIANGLMADGILTGGKRKLWRGEGVRLILRNEKYMGDALLQKTYTTDFLTKKRVKN
                     DGSYAQQYYVEDSHPAIIPKDIFMQAQQELDRRKSMKNKKSQCFSGKYALSGITVCGD
                     CGNAYRRVHWKNRGTVWRCKSRVDKREHNCTGRTIYETDLHGAVIKAINETLIDRDAF
                     LQQLNDNINSVMTDGLTDRLAELDSQLKELEAEIISLAVGGQGYDELASQIFSLRDER
                     DAVAKQIAANTNLQQRVDEMVVFVKEHDVINEYNEVLVRRLIEKVTIFEKNIVVDFKS
                     GVRVTVEI"
     gene            complement(2198..2563)
                     /locus_tag="ACHGMT_000003"
     CDS             complement(2198..2563)
                     /locus_tag="ACHGMT_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN6833785.1"
                     /translation="MDEQQAQQIREFFNIYLEGVGLQEAGKRSGINKPHPFMKRILMN
                     EVYLGNEIYPKIIDEDIFFQADLEQERRIKFFKKRKEIKVPDTKVIPTHFKSGKITEK
                     YSDPFKQAQYAYSKIKEVK"
     gene            complement(2611..4164)
                     /locus_tag="ACHGMT_000004"
     CDS             complement(2611..4164)
                     /locus_tag="ACHGMT_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN6833786.1"
                     /translation="MKTIHKLARPELPEASKLKVAAYARVSTSSNEQLASLQTQITHY
                     ENHIKNHEQWDYVGVYYDEGISGTKTVKRDGLKRLIKDAELGKIDLILTKSISRFSRN
                     TVDCLNLVRQLTDIGVTIFFEKENINTGDMESELLLSILSSLAESESYSHSENLKWAI
                     RNRMSKGKFKAYPPYGYKRKGYDFHIVAAEAKVVKQMFQWALDGISTGMIAKRLNDGK
                     QFTKRGANWQATSVRSILKNEVYTGTLTMQQTFVDDQYKRRINKGELPMYQIKNNHEP
                     IVSKEDYDRVQELIRRKAEAKGNTGDGKKYTKRYAFSGNIICDKCGSKFKRVHTYWKG
                     NTKKVQWKCTGHLKNKDSCDALPITDEDLKVAYLTMLNKLIFGHKLVLEPFMKSLNKA
                     GANQEGLEVLADEIDKIQEKLEVLASLTASSILSTKVSLEEQSKLQQERAVAQEKQRL
                     IIESVNGKSTQQIELEALYKFARMAEMLTDWDEDLFKKFTNSITVYNRQEVGFELKCG
                     LLLKERLPE"
     gene            complement(4225..4398)
                     /locus_tag="ACHGMT_000005"
     CDS             complement(4225..4398)
                     /locus_tag="ACHGMT_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HFN6833787.1"
                     /translation="MTNKKTEINYSDEAAYYISQQILLNLLVTGKINLEEYVKIDNKN
                     RETFNPFLSRIMV"
     gene            4693..4998
                     /locus_tag="ACHGMT_000006"
     CDS             4693..4998
                     /locus_tag="ACHGMT_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019299341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6833788.1"
                     /translation="MSSFDMEGLEKEYEHFCKSATTKELEEFDESFKQEFEKCFKTIE
                     AEEALYQFLQANINAMNNRMESIESPLFCINYKEQVFQLRNHLILMQNAVNTYLKEN"
     gene            complement(5059..6096)
                     /locus_tag="ACHGMT_000007"
     CDS             complement(5059..6096)
                     /locus_tag="ACHGMT_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014572406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HFN6833789.1"
                     /translation="MVTFKDLLPEPTESQQAFILDHGKTEDDGQLKYADDAKSYGWNM
                     RQYGKLKAGAVVLNRHPGKITKDRKWEIYGGGYVESISEPDEAGNVTAVITHAFTIEP
                     AIKQGDSFIENFDWNTPSKKKRKKPNSWAYFWDQYGMNEITYADFVALIENRHLIPID
                     DTQSLPSELDLTNEEVEEIEQESSKGFTVLVDEVGPNRTNGTQKRKFTGRHTDWERVN
                     KAKQKTGALGEEIVLDFLIQEAEKNKTKLPEHVSKTEGDGHGYDICAFDQSGNEIHIE
                     VKASKTNFSDGFEMSTNEVAASLGDTPYKIYFVHDLDVASKVCKIKIYDGPITEENFT
                     MVPTSYKIFNK"
     gene            complement(6212..7120)
                     /locus_tag="ACHGMT_000008"
     CDS             complement(6212..7120)
                     /locus_tag="ACHGMT_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFN6833790.1"
                     /translation="MGISNLATKYGFQSFSHYSNGRSGAKVDKIVIHHMATTNYDIVP
                     DIWKTREASAHYGIGKNGEIRAYVDEGNTAWHAGNWNANISSIGIEHCNSTGSPNWGI
                     NQATIDASAKLCADIAKRYGLGKLVVNKNIFPHSHFSATSCPGQLLGKLQEIADKANA
                     INNGSSTPSTPASGLYRVRKSWADAKSQIGAYKDLNNAKRAADSHSGYKVFDQNGNQV
                     YPSGSSTSNVKTLVVQVSGLNVRNKPSLSGAVVGSYNKGATWTLPKSEKMTIADGWVW
                     ARCPIGYCAVGKNTGKTESDDYILIS"
     gene            complement(7110..7529)
                     /locus_tag="ACHGMT_000009"
     CDS             complement(7110..7529)
                     /locus_tag="ACHGMT_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin family protein"
                     /protein_id="HFN6833791.1"
                     /translation="MKEVWNWIQLAIAGGGGVIGWYVGGIDGLIYTLVTFVVVDYVTG
                     LLRAINEKKLSSKIGAKGITKKIMIFLLVGVAHMLDVEVIKGGNVLRDAVIFFYISNE
                     GISLLENAVAIGLPVPEKLREILQQLHEKKEETENGN"
     gene            complement(7530..7937)
                     /locus_tag="ACHGMT_000010"
     CDS             complement(7530..7937)
                     /locus_tag="ACHGMT_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1617 family protein"
                     /protein_id="HFN6833792.1"
                     /translation="MLKIRNEDLVDVMSFLKGFDLPPKISRVRTKLAKLIQVKVDELY
                     QDEVDLLQKYGKKDEHGNLVQHDGAFSLIPETATEYHKEKADLLNEETHINVDELQDK
                     LPLLVAGFENSEVTISGKDAEMLDLILEQLEDL"
     gene            complement(7924..8091)
                     /locus_tag="ACHGMT_000011"
     CDS             complement(7924..8091)
                     /locus_tag="ACHGMT_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6833793.1"
                     /translation="MNEEKEEVIVTEPIEADIPKPMKVGTDMVDLLVMVVDDLCVQIG
                     DLKEKIDNAQN"
     gene            complement(8101..11991)
                     /locus_tag="ACHGMT_000012"
     CDS             complement(8101..11991)
                     /locus_tag="ACHGMT_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFN6833794.1"
                     /translation="MITLYNKTESNFNHNGLAVLDGNILNPVVSEELNGLFSFEFDYP
                     IHAPHSEELLPEMIVKAPVPELSDQLFRIVERDDALGGFLHIVAHHIFYDLATNFIED
                     TYIVNKNGSGALTQLLGATQFMHRFTGTSNISAVNNVRLVRLNPVEVLLDSDLENGFQ
                     SSYGGEIVRDNFTISMLTTRGSNNGVQIRDKKNLTGYKADVDYSGIVTRIMPQGYDGL
                     FLPEKYVDSPNINQYVQPKIKVIKYDNVKVGDEEDEFTQAEAYDELRRLAGLEYSANK
                     VDLPKATYDVEFAPLERTEEYKDFAQLETINLGDTVSVIHAEDGLNITARMVSYKYDP
                     FLKAYISVTLGNITPKFTDVAKDINRVDNKAEQAKDDANYALSSANGKNTNFYGPTTP
                     NNPKLGDVWYKENGDKFEMWVYEIRDGVTQWYPLMTDMTAEEVKQAVEQAQKDANTAT
                     EKANQAFNDAVNALNAAESVGASVETLTTSVEQNTSKISLVASGMSTLEGRVITAEST
                     LTVQAGQIASKASQSTVDNLTGRITTAESTITQNADRFELSLSKTNKAVNVLAGMSIA
                     DLQWAVGGLNTNDGKENTYGGYVRSGFTRVRNGEKYLLQTFVGGKLYSTYATAYLLYY
                     KEDKSFLSYTQFGNTTTPLTVPSSAAFIRIRLTTSDAPETISCYMLKTDVTNGYVDLS
                     EISNVVKLTATTDALLLSVSETKEAIGVPFKVNQWEQGSLSTTDGSEVSATNYLRSEF
                     LDVKVGDKYIGQLRDGTQAAFYYHYYGMDLPYIDFVPTAKQFSETLTAGTYTNATIAS
                     YLVSKNVESMSITSGVTTNPYSNSGDYLVIYKLPLEGKVAPSVISWNGRKDTSDNIVL
                     LYTGGAWEQISTVTSSSNATYSWTLTAEQISGITTDSVYLAFFSVKTGTYAGIYNSSN
                     TPFTLNPSDGSSYHQISYTGSSGAITVPSGTLKMRVRVNTSIKPDEYEGNIYPTTTRQ
                     DYTNANTLYSAILMQKDLINLRVGKGDVINQINISPESILIAGNKVHITGQTTIDSAV
                     ISSAMIQNAAITNAKIADATIQSAKIASIDAAKITTGTLSAARIGANSITADKIAANI
                     LTALTASSSIRITGTTIGYYSGSTLVTEINSQGMTIRRGGTTVGTIGANNISGHSDWR
                     GLVFDLEYGTEYMSWAHKDSSSASVYTQKLSWYARTLQSGMEKGFHFDDDIYLPSMLR
                     IGTQHQISVGGITFSSSLYTKIGSSSGSAGIAFNGSNLMLGDDGKWVDFGVIREICAK
                     LAGRTIALPTGFSSNGTASGWYNPQSFNSMTTYS"
     gene            complement(11988..12680)
                     /locus_tag="ACHGMT_000013"
     CDS             complement(11988..12680)
                     /locus_tag="ACHGMT_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFN6833795.1"
                     /translation="MNAFILDGTINSRTDLGLRITEVPVIPVTERVIDSIEIDGREGD
                     LTILKGWKDVTFGFKVVIWKSDVWTSWQIILPQILNAKTIAFSNEPNVFYPIKYAKAS
                     GLTQILSSMWEFEMELTCSPFRYKTNVATINRTTSGTVNNPGNIYSLPKIKVYGNGTR
                     TLTINGKPIVLNLTNGNLTLDSDLKECFYGDVAANQYMTGDFPEFRVGSNTVTLGTGI
                     TKVEIEPRWRYL"
     gene            complement(12757..13158)
                     /locus_tag="ACHGMT_000014"
     CDS             complement(12757..13158)
                     /locus_tag="ACHGMT_000014"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6833796.1"
                     /translation="MLKHKFKAKKPALIGYILLLAIGLFLTAVRWYSGINHGFVFINN
                     QINSHISNFALSFLFYLVIGYLWLLMGTNLRVLTAMGAGLIVANIVCETMMTFLNTPD
                     LIDAIYGISGVLVVYAYLAITKKIGLEETTE"
     gene            complement(13229..15790)
                     /locus_tag="ACHGMT_000015"
     CDS             complement(13229..15790)
                     /locus_tag="ACHGMT_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFN6833797.1"
                     /translation="MADNFGLKIGVEGEKEFKNSLREINQSFKVLGSEMKLVSSEFDK
                     NDKSISSLSARNQVLNKSIDAQKEKITTLENALKNASDSFGENDKRTQNWTVQLNNAK
                     AQLNNMEREVANNEKAIDAMGTEEVDAAKKTDELGNELKDTGDEAEKSGSKFEKFGGV
                     LKGIGVAMGTVALAAGAAAVELGKQVVSAYADYEQLVGGVDTLFKDASSTVQKNADNA
                     FKTAGMSANEYMETVTGFSAALISSLGGDTKKAAGVADQAVTDMSDNANKMGTDIESI
                     QNAYQGFAKQNYGMLDNLKLGFGGTKSEMERLLQEAEKISGVKYDISNFADVTEAIHV
                     IQTEMGITGTTAKEASETISGSIDGMKSAISNLMAGLGNSSADIGMLIGNVVEQFQNV
                     IKNILPVIQNIVSALPAVVDAIIQALGQLLPTLLEAVTSLFSQVLETILGLLPTLIPV
                     VVQAVLTIVQTLIENLPLLIDAAFQLVTTLIQGLGEALPELIPITVQAIITIVNGLIE
                     NLPLLLDAALQLIMGLAQGLITALPILIESLPQIITGIVNFIISAIPQIIQTGIQLLT
                     SLVTALPDIINAIVAAIPVIIQNIINAVIGAIPQLIDAGIQLLVALIGALPQIITTIA
                     NAMPQIINGITGTLVNNIDKIINAGVKLLVALVQNLPAIISAIVKAVPQIITGLANAF
                     GEYVSTMGTIGKNLITGLWNGIADAGSWLRNKISGFFGGVVDNIKDFFGIHSPSTLFR
                     DEIGKYMAQGIGVGFDKEMTGVSKTMQKAIPTKLYMPSFDIDTDIHAAVDSTFSMLDL
                     GAKVDALTNITSEMLPTLLKAMDIKVVLNDGTLVGKLAPEIDKSLATLNRRRVAY"
     gene            complement(15809..15949)
                     /locus_tag="ACHGMT_000016"
     CDS             complement(15809..15949)
                     /locus_tag="ACHGMT_000016"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6833798.1"
                     /translation="MGMTSDDFWYCSLGLFLDLWECHKQFTGISKPKVELFIDDVIPS
                     GI"
     gene            complement(15997..16377)
                     /locus_tag="ACHGMT_000017"
     CDS             complement(15997..16377)
                     /locus_tag="ACHGMT_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6833799.1"
                     /translation="MSEERSAKISLGGDEYELILTTKATKEIASHYGGLDNLGEKLLK
                     SENFELALDEIIWLITLLANQSIKIYNLKNTEKKDELTTEYVELMTSPLQLAEYKSAI
                     TEAMFKGTARNIESETDSKNKAGE"
     gene            complement(16387..16977)
                     /locus_tag="ACHGMT_000018"
     CDS             complement(16387..16977)
                     /locus_tag="ACHGMT_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFN6833800.1"
                     /translation="MATIGLDKLYYAPITESATTGEETYGTPAMLAKAISAELSVELA
                     EATLYADDGASEVVKEFKNGKLTLGVDDIGRAVAAVLVGATVDQNGVLISTTEDGGTP
                     VAIGFRARKSNGKYKYLWLYRVKFAVPSTSLATKGDDITFSTPTIEGTVMRRNKLDAS
                     GKHPWKAEVDENDTGVEQTTINGWYTSVYEPDFTAD"
     gene            complement(16980..17321)
                     /locus_tag="ACHGMT_000019"
     CDS             complement(16980..17321)
                     /locus_tag="ACHGMT_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6833801.1"
                     /translation="MNILEELNSLFATLHIPVETGEFSDKAPDKYCVLTPLADRFDVY
                     GDNFPTVDVQEVRISLFVKGNYLNTQKQLVQNLLKHEFTITERLFVGFEKDTNYYHVA
                     IDVAKHYEMEV"
     gene            complement(17318..17749)
                     /locus_tag="ACHGMT_000020"
     CDS             complement(17318..17749)
                     /locus_tag="ACHGMT_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFN6833802.1"
                     /translation="MAKALMKMPEDFLLKVSKLENKTDEIIPKVLESGAEVVEGKVRS
                     NLTAVVGSNTKVPSRSTGELESALGITQARQDKSGNWNIKVGFDEPRIDGSSNAKIAN
                     ILEYGKHGQPAKPFLKPAKSQSRKSCIETMKSKMESEVNGI"
     gene            complement(17742..18080)
                     /locus_tag="ACHGMT_000021"
     CDS             complement(17742..18080)
                     /locus_tag="ACHGMT_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFN6833803.1"
                     /translation="MSFGKMNQRIQIVKVTNQKDSAGFSVKQDEVLAAVHAYKEEKNA
                     TEKWVNMATFSSATCLFRIRYIPNLEVTTEMVVISDQKRYQIVSVENVRNRNMYLEML
                     TTEMEVSADG"
     gene            complement(18077..18388)
                     /locus_tag="ACHGMT_000022"
     CDS             complement(18077..18388)
                     /locus_tag="ACHGMT_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFN6833804.1"
                     /translation="MSDLLNKVKANLILEHNEDDELLERLILTALSYAESYQHIESGY
                     YSEHEMPPTTEQAVIVLASNFYESRDGSTGGFFADNVNASKQVWETVNLLLRLDRRWQ
                     V"
     gene            complement(18399..19610)
                     /locus_tag="ACHGMT_000023"
     CDS             complement(18399..19610)
                     /locus_tag="ACHGMT_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFN6833805.1"
                     /translation="MNKIQELMEKRAQAWNGAKAFVEAKQDKDGLMSEEDAKTYAEME
                     TKVKNFTMEIERMQSMEAMDRELEKPMSQPLTSQPMTTENKMEPKKTGRASAEYKEGV
                     LEALRSNFRHVSNVLQEGVDTAGGYLVPEEYDSRLIDGMTSENIMRTLATTITTSGEH
                     KINIAGSKPAASWIEEGGELSFGDATFDQILLDAHKLHVAIKVTDELLYDNAFNLENY
                     IIDQFTKALANAEEDAFLNGDGVGKPLGIFAATGGGQVAITTNTQSAVTADDVVNLVY
                     ALKRPYRKNAKFIMNDQTISLLRKLKDNNGAYMWQPALVAGEPDRLLGYPVYTSEFVP
                     TVKAGAPVIAFGDFSYYNIGDRGVRSFDQLRELFAGNGMVGFLAKERVDGKLVLPEAV
                     QILKMKASASS"
     gene            complement(19614..20369)
                     /locus_tag="ACHGMT_000024"
     CDS             complement(19614..20369)
                     /locus_tag="ACHGMT_000024"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFN6833806.1"
                     /translation="MKKFWKWKNQTVLNQDNQEEILERTLYLNGPIAEESWFDDEVTP
                     QIFKDELNSGGGNVTVWINSGGGDCVAAASIYNMLADYKGKVTVKIDGIAASAASVIA
                     MAGDKVLMSPVAMVMIHNPSAVAFGEKVDMEKAIAMLDEVKASIINAYERKTNLSRVQ
                     LAHMMDAETWMDANKAVHLGFADEIIGEIDPDIPVNNSMYSEVKVTNSLTSAIAKKCR
                     IEPPEPKEREEINNTIPADSLMSRLNLMKNWRN"
     gene            complement(20269..21597)
                     /locus_tag="ACHGMT_000025"
     CDS             complement(20269..21597)
                     /locus_tag="ACHGMT_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFN6833807.1"
                     /translation="MGIIKSLFKSRDKPTNQTIMNPYRFMFGGSSAGKSVTEQTAMQM
                     TAVYSCVRVLSEAVAGLPLHFYQYRDAGGKDKAMKHSLYFLLHDEPNSEMTSFVFRET
                     LMTHLLLWGNAYAQIIRNGKGEVVALYPLMPDKMTVNRDDNKEIYYLYQADVGQVRLS
                     KADVLHIPGLGFDGLVGYSPIAMAKNAIGLAIATEEYGSKFFANGANPGGVLEHPGTL
                     KDPGKLRESWNATFGGSSNAAKVAVLEEGMKYTPISMSPEQAQFLETRKFQLEEIARI
                     FRVPLHMVGDLDHATFSNVEHLSLDFVKFSLSPWLNRWEQALNKSLLSRDEKEKYFIK
                     FNVDGLLRGDYATRQQGYATGIQNGFLSPDDVRELEDLNPIPGGAGAKYYFNGNMLPI
                     EQAGAAYGVNKEESEEQDEEVLEVEESNGSESRQPRGNPRENPLPQRTNR"
     gene            complement(21663..22397)
                     /locus_tag="ACHGMT_000026"
     CDS             complement(21663..22397)
                     /locus_tag="ACHGMT_000026"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6833808.1"
                     /translation="MNEYLIQVAPDKISSDPDNTLISRLFYRKWWGKILTEDLTKYAE
                     GIGNYRGETIISFNTIAGATIRLLKCYNQKNFSMPDSHYGRLKVILYSDDITEEMKVK
                     FVKFYDLSHTLANFMPLYVSKPSLNGVKGKGPYHDFPDLFYSDVRDKRYGSSTNADKF
                     IVNNKDYFSKFKDWKSFIELNYLQDFFDDEDYMTFKKLAPSSSFFPYQRKSLTPDEKS
                     KCLEEVNTFLNNAIEIILSRGSRFYI"
     gene            complement(22453..24018)
                     /locus_tag="ACHGMT_000027"
     CDS             complement(22453..24018)
                     /locus_tag="ACHGMT_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFN6833809.1"
                     /translation="MDKDSHYDKSRADYAVAFIELLKHTKGQWFGKPFKLMPWQEKII
                     RDLFGTIKANGYRQFNTAYVEIPKKNGKSELAAAVALLLTCGDGEERAEVYGCAADRA
                     QASIVFDVAVDMIRQNPALSKRVKILTSQKRIIYQPTNSFYQVLSAEAYSKHGLNSSA
                     VVFDELHAQPNRKLFDVMTKGAGDARTQPLFFLITTAGNDTNSICYEQHQKALDIIEG
                     RKHDKTFYPLIYGIKQDDDWTDPEVWKKANPSLGITIAEEKVVAACDSAKQNPAEENS
                     FRQLRLNQWVKQAVRWMPMDKWDACSFNADEESLRGRVCYGGLDLSSTTDLTSFVLVF
                     PPEDEDDKFVILPYYWVPEDTLDLRVKRDHVNYDLWAKQGYIQTTEGNVVHYGFIEKF
                     IEELGEKYNIREIAFDRWGAVQMVQNLEGMGFTVVPFGQGFKDMSPATKELMKLTLEQ
                     KLAHGGHPVLRWNMDNIFVRTDPAGNIKADKEKSTEKIDGAIATIMALDRAIRCGNDN
                     GASVYDDRGLLFL"
     gene            complement(24101..24244)
                     /locus_tag="ACHGMT_000028"
     CDS             complement(24101..24244)
                     /locus_tag="ACHGMT_000028"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6833810.1"
                     /translation="MMDYQIYMPDNEDGIEEGYYDWQELVQLLRDNKNNPEAIQFIAD
                     MME"
     gene            complement(24241..24417)
                     /locus_tag="ACHGMT_000029"
     CDS             complement(24241..24417)
                     /locus_tag="ACHGMT_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5049 domain-containing protein"
                     /protein_id="HFN6833811.1"
                     /translation="MNAKIKQQIIEIAESGLTNMFDAIAVQRIAHDRHFYELVIFIEE
                     HKDEYSRFILEGDK"
     gene            complement(24401..24595)
                     /locus_tag="ACHGMT_000030"
     CDS             complement(24401..24595)
                     /locus_tag="ACHGMT_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6833812.1"
                     /translation="MKVSKRKIYNIAKNYIVQLPERGDLKAHNSDREDFLDIAVWCLE
                     DALIAAYEAGRKDGQNERKD"
     gene            complement(24597..24974)
                     /locus_tag="ACHGMT_000031"
     CDS             complement(24597..24974)
                     /locus_tag="ACHGMT_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4314 domain-containing protein"
                     /protein_id="HFN6833813.1"
                     /translation="MFPTHEQIEGMKKRYPEGSRVELIKMNDPHAPAKGTQGTVTGVD
                     DIGSILIQWDNGSTLNAVYGEDVVHIIKPDFELVFQNGRREGFETFNDAWQYVTDMIL
                     NHDLVWVDLESKTEDTIYIRKGL"
     gene            complement(25048..26283)
                     /locus_tag="ACHGMT_000032"
     CDS             complement(25048..26283)
                     /locus_tag="ACHGMT_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFN6833814.1"
                     /translation="MNTTERFEKVDIDKLVPYARNARTHSKDQVKQIQASMREFGFIN
                     PVIVDKDYNLIAGHGRILAAKEEGIKQVPCVFVEHLTEAQKRAYILADNRLAMNAGWD
                     DEMLAVELSDLQGEEFDLDLLGFETDELDKLLNGNDDAQDDDFDVDEELEKPAFSKTG
                     DLWLLGRHRLVCGDSTKPETYEKLMDGKLANLVVTDPPYGVSYQGKAGTIKNDNLKDE
                     AFYNFLYDAFSCMESVMANDASIYVFHADTEGLNFRKAFVDAGFYLSGTCIWKKQSLV
                     LGRSPYQWQHEPVLYGWKKKGKHQWYTGRKESTIWEFDKPKKNGDHPTMKPIPLLAYP
                     ITNSSLSNCIVLDSFGGSGSTLIACEQTDRICHTIELDEKFCDVIVKRYIEQAGSDAD
                     VSVIRDGKTIAFNDLENPE"
     gene            complement(26283..27065)
                     /locus_tag="ACHGMT_000033"
     CDS             complement(26283..27065)
                     /locus_tag="ACHGMT_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HFN6833815.1"
                     /translation="MIEKVNPQHPDKVADRIAGAIVDLAYAEENNPKIAVEVLIGHGI
                     CHVIIETSTALNLNEISQAINRIAGKIKKDVVIVPQDKELADNQATEIRCGDNGIFRG
                     MPLTDEQLQLSKIARDLYQDHPFDGKYILDGERLIICQSHAKTNELKQKYPNATVNPL
                     GDWTGGPDVDTGATNRKLGSDMADGVTGGGIHGKDLSKADVSVNIYAFLKAQETGMPV
                     ELSCAIGDTEIDGVPYSEIVEVARDYVNELGGFEKLAEWGLF"
     gene            complement(27080..27652)
                     /locus_tag="ACHGMT_000034"
     CDS             complement(27080..27652)
                     /locus_tag="ACHGMT_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFN6833816.1"
                     /translation="MAKDGTQRGGRRPRSGERSQPLVDKVAAGKKASRMEFDLPDVEL
                     LIGEDIGEGVELEGNDYPEPSAYLSAKQKDGTVLGADEIYNSTFQWLKDRGCETLIAP
                     RMVESYAQNFARFVQCENALSQYGLLGKHPTTKAVMASPFVAMSQNFQKQANVTWYEI
                     QQIVKENASVEYTGDPNTDAMERLLRSKNG"
     gene            complement(27786..28151)
                     /locus_tag="ACHGMT_000035"
     CDS             complement(27786..28151)
                     /locus_tag="ACHGMT_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFN6833817.1"
                     /translation="MPMKPANPCKFPGCPNRTHDTYCDEHKTLARKRYEKYERDPAIN
                     KRYGRAWKKIRARYVAAHPLCEMCLKEGRFTSVEIVHHIKELSDGGTHDENNLMSVCK
                     SCHSKIHMTRRNTKDKTIM"
     gene            complement(28319..28747)
                     /locus_tag="ACHGMT_000036"
     CDS             complement(28319..28747)
                     /locus_tag="ACHGMT_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HFN6833818.1"
                     /translation="MTPKEYLKQAYRLNKRIDDRIKEKAELYQLAVTVSSTTFEEKIS
                     SGTRNTEAPFVRNLEKCYQLEAEIDAEIDKFVDLKRQMRGVIDELTNVDEKIVLINRY
                     LLEESWDKIAEDLHADVRTVFRWHSKALENLKLPDEPIVI"
     gene            complement(28744..30096)
                     /locus_tag="ACHGMT_000037"
     CDS             complement(28744..30096)
                     /locus_tag="ACHGMT_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HFN6833819.1"
                     /translation="MKFVPHNYQKYAIDFIKDHKTAALLLDMGLGKTVTTLTAIKDLM
                     HDDFTIQRVLIIAPLRVTQSTWPTEIQKWDHLKDLSYSVVLGTPSQRMAALQNNANIY
                     LINRENLDWLINKSGFTFDFDMVVIDELSSFKNLKAKRFTSFMKVRHKVDRIVGLTGT
                     PSSNGLMDLFAEFKVLDMGERLGYYISRYRDKYFLPDKQNGMQIYSWKPREDAEQEIY
                     DKISDITISMKSVDFLDMPELVMNEVPVDMGLKEKQKYDEIKADLVLQLKEATIDAAN
                     TAVLSNKLLQMANGAIYDEFNVSHHIHDQKLDALEDLIEGTNGKPILIAYWFQHDLER
                     IKERFNVRQIKTSQDIIDWNEGSIPIAVIHPASAGHGLNLQAGGSTLVWFGLTWSLEL
                     YQQTNARLWRQGQSDTVVIHHIIAKDTIDEDVMLALKRKDKTQSCLIDAVKARLEGSK
                     "
     gene            complement(30096..30380)
                     /locus_tag="ACHGMT_000038"
     CDS             complement(30096..30380)
                     /locus_tag="ACHGMT_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFN6833820.1"
                     /translation="MREKQVEQALVKAVKRVGGICPKFTSPGLAGVPDRLVLMPNGKL
                     GFVEVKAPGKKPRSLQLFRMKQLTDLGFQCFVLDEIDQIPELLERIGGDV"
     gene            complement(30377..30586)
                     /locus_tag="ACHGMT_000039"
     CDS             complement(30377..30586)
                     /locus_tag="ACHGMT_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6833821.1"
                     /translation="MKFKQWLQIGIGKRDLRFDEFYEFAMSDVTYPWKKSFDKQVAHL
                     MIHCAEKEYLEILRDAYFEYLTVWL"
     gene            complement(30724..32967)
                     /locus_tag="ACHGMT_000040"
     CDS             complement(30724..32967)
                     /locus_tag="ACHGMT_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFN6833822.1"
                     /translation="MLKMTLFTSNQVGNSKNGLFPTKQVVTDKETLVATVKLDHVMAE
                     YKGNYRKGDNFIQSDVIPLDCDNDHSDNPDDWVAPFEVAMQFPDVTFAVSYSRNNMKD
                     KGGKSARPRFHVYFPIPATTDKDEYVELKKQIQSEFPYFDNNAMDAARLLFGTPNSEV
                     EIYEGSKLITDFLEDDLFADWDNAQSEISEGSRNSSMSHIAGKLIKRYGATEDTYHKY
                     LEQSQKCNPPLPEEELKAIWNSAVNFGKKVKQQEGYIPPEQYNSDLSLEPTDYSDLGQ
                     ATVLAREYECKLHYSPSTGYLVYNGSYWEESKPKAQGVIHALTERQLEESETEIEKRT
                     KEMVSNGAFDVLASVGPKKAVTMFNTAQRHSFDLYQHAQEYKKFAVKRRDSKYLSSAL
                     TEAKPMLEIEQRLLDVNEFLLNTPTATFDLRTGKSQDHNSEDYITKQTECAPSDANQQ
                     IWLDALNTIFVGDQELIDYVQMIVGLAAIGKVYVEALIISYGEGRNGKSTFWNVISRV
                     LGNYSGSISADILTSQIRRNVKPELAEAKGKRLLIAAELEEGMRLNTSNIKQLCSTDE
                     IAAEKKYKDPFKYVPTHTLVLYTNHLPKVGAIDKGTWRRLIVIPFLATIDGNKDIKNY
                     ADYLFENAGGAILQWILDGAKKVIAADFHLTVPQVVANAISEYKTANDWLGHFLDECC
                     ETAVDFEEKSGELYAEYRAFCTRTGEYIRSSADFYTALEAGGFKRRRTNKGNKIVGLQ
                     LKSEFLE"
     gene            complement(32960..33304)
                     /locus_tag="ACHGMT_000041"
     CDS             complement(32960..33304)
                     /locus_tag="ACHGMT_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFN6833823.1"
                     /translation="MPRRKDYRTSVYVCSPFSGNVVVNHKNTKRYCRIALDRGYMPVA
                     AHLMYPQFMDDATERDVAMHMSLVLMGKCEEVWVVGDTISQGMAVEIEQAEYWKKHIR
                     YFDDEMKEVSNA"
     gene            complement(33297..34067)
                     /locus_tag="ACHGMT_000042"
     CDS             complement(33297..34067)
                     /locus_tag="ACHGMT_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HFN6833824.1"
                     /translation="MNELKVFENAEFGSVRTVMIGDVPHFVGKDVASILGYTNPQKAL
                     RDHVDEEDKTVNESFSVNGTMAILINESGLYSLIISSKMPNAKKFKRWVTNEVLPSIR
                     QHGVYATEDFITKSIEDPAWAISVLKQLQEKKEMVAMQQQMILEMKPKVSYYDLILQN
                     NSVMSISKISKDYGMSPQAMNKLLHELGIQYKQGKMWLLYQKYANQGYTQSKTHAIDA
                     ERSVMHTYWTQKGRLFLYDLLKSKKNILPVIERGDNNA"
     gene            complement(34278..36215)
                     /locus_tag="ACHGMT_000043"
     CDS             complement(34278..36215)
                     /locus_tag="ACHGMT_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HFN6833825.1"
                     /translation="MKHLSLDLETYSSVDLSKCGVYKYSESPDFEILLFAYSVDGGPV
                     EVVDLKMDETIPAEILTSLSDETVIKHAFNASFERVCLSRFLGLDGFLNPRGWQCTMV
                     WGATLGLPLSLVGVGAVLGLDKQKLTEGKDLIRYFCVPCNPTKVNGGRTRNLPHHAPT
                     KWSAFKTYNSRDVEVEIAIQGKLSNFPVLDSVWKEYWLDQEINDRGIEIDKDFVEAAV
                     QLDELSKAGLNQQLQELTGLENPNSVLQMRGWLLDHGLETESLGKKAVAELLEQPIDE
                     NLKQVLVLRQQLAKSSVKKYQAMQNFACSDNRSRGLFQFYGANRTGRFAGRGIQLQNL
                     PQNHMSDLSEARSLVKQSNFEMLNILYGNVPNVLSELLRTAFVPREGMKFIVSDFSAI
                     EARVIAWLSGEKWRQEVFKNGGDIYCASASQMFGVPVEKHGVNGHLRQKGKIAELALG
                     YGGSVGALKAMDALEMGLTEDELQPLVNSWRDSNINITELWWAVDKAVKTCVKERRST
                     ATHGIKFEYKSGFLFIQLLSGRKLVYVKPKIGENKFGGEAVTYEGVGATKKWERLESY
                     GPKFVENIVQAIARDILCFAMENLRKYRIVGHVHDEVIIEVPMESKVEDICQLMSQTP
                     KWAEGLLLNADGYECEFYQKD"
     gene            complement(36276..36818)
                     /locus_tag="ACHGMT_000044"
     CDS             complement(36276..36818)
                     /locus_tag="ACHGMT_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HFN6833826.1"
                     /translation="MSKTSKVITGTNTRLSYFHGWEPASINGGAEKYSTAVLIPKSDK
                     RTIDTIEKAIDAAIEEGLAKFGGKKPNKAEIKLPLRDGDIERDDEAYQGHYFINANST
                     TAPQIVDQHVQPILDRSQVYSGCYARVSINFYAFNANGNRGVAAGLGNIQFVRDGEPL
                     GSQVSASDEFETLADDDFLA"
     gene            complement(36820..37962)
                     /locus_tag="ACHGMT_000045"
     CDS             complement(36820..37962)
                     /locus_tag="ACHGMT_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HFN6833827.1"
                     /translation="MPTQHAVLSASSSNRWIHCPPSVRLSEHFENKSSPYAQQGTDAH
                     TLCEYKLNKFIGNDVTDPTSTLEFYDEEMEQCAESYATFVMEEVAKAKQTTVDSIVIV
                     EQRLDFSRYVPDGFGTGDCLVIADGTLSVIDMKYGLGILVDAYQNPQMMCYALGALEL
                     FDVIYDIQEVKMTIFQPRRENVSTYTLLKEELLQWAKDVLSPAADLAFKGEGEYQCGK
                     WCGFCPAKNSCRARADHNLKLAQYEFKPPELLSDDEIEEIIGKVDDLVSWSNDIKDCA
                     LKLALGGKQWTHHKLVEGRSTRKYSNDNDVAAAVIKAGYDPYDKKLLGVTAMTKALGK
                     EKFNEILGEYIIKPKGKLTLVDGSDKRQAVTVNNLNEEFKPLTEEQ"
     gene            complement(37963..38277)
                     /locus_tag="ACHGMT_000046"
     CDS             complement(37963..38277)
                     /locus_tag="ACHGMT_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA biogenesis protein rrp5"
                     /protein_id="HFN6833828.1"
                     /translation="MSKTKLFLQVVEDVQALADSLQELAVAMKTNEPEPQVETPEPTM
                     ETKSEVKQPTLEEVRGLLARKSQEGKSTEVKALIEKYGASRLSDVPTENYAALVADAE
                     GL"
     gene            complement(38282..38488)
                     /locus_tag="ACHGMT_000047"
     CDS             complement(38282..38488)
                     /locus_tag="ACHGMT_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6833829.1"
                     /translation="MNLKHKIRVNVTDDTGNKETVLQGGVRHLPRWFARWLFGKGNMD
                     KILVLTPGDTIESVEIREVNKKGA"
     gene            complement(38585..39202)
                     /locus_tag="ACHGMT_000048"
     CDS             complement(38585..39202)
                     /locus_tag="ACHGMT_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6833830.1"
                     /translation="MSRFMKTHQNDRSTYKYVFERTDANGRIQKEVVEIRPGEDGVTE
                     LNIQELHRADDRAIYNFYKNARPERTEEEKEKIKAWKEKFREDFNNEHGYYPMDEYVD
                     GEANDYFKRNYNLSLNEFDDECDEGDNPIQSLVYDAYQDNEEDPRIDRLREVVTQLLP
                     YHQEIYHMLVTENMSQTEVADKLDKKKQSINRTFGIIKDKIKELF"
     gene            39597..40547
                     /locus_tag="ACHGMT_000049"
     CDS             39597..40547
                     /locus_tag="ACHGMT_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system protein"
                     /protein_id="HFN6833831.1"
                     /translation="MEISEITQTLYPFMGSEYVKPQDFLIALLDSVSNDDEATIFGFS
                     DDNLRRFYNGTRPLTQEYAVRVAGKFNIDKCTTFIYERLIDDAPTVLKQQLIDKGVVI
                     PKDTDVSEIVAHLLLDEITSIASQTKVKTKKAKPKLSELELASLRINTNGDFSYNDEV
                     KKFFDDLPVPLDIRAEEMTYVIALFSAYADADGYEVYEHTNDLPNSRLKDFKRQRRNY
                     YKAESIRRGVRDNFTQEEGTEHFEALKEDMYDGIEEVYEEDYDDGVERLKAVLQQSSV
                     ITLNGSILSFIPGLLQNSAKKGICHMLVNDGKIKWVTEDE"
     gene            40540..40986
                     /locus_tag="ACHGMT_000050"
     CDS             40540..40986
                     /locus_tag="ACHGMT_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system middle component 2"
                     /protein_id="HFN6833832.1"
                     /translation="MNSLFNTPFEISVRVMLLLSEFKEPVSADRILITDFITTYGRDF
                     EISDYNLNGDNSYRFSEFIARRELVKDAVKNLVLQHVIQPSQSKEGFKYALSEDGLKL
                     ISQFVSVYAEKYLQIADESVKYIQDKSDVELLRQINQRAKEFKGEQ"
     gene            40989..42821
                     /locus_tag="ACHGMT_000051"
     CDS             40989..42821
                     /locus_tag="ACHGMT_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN6833833.1"
                     /translation="MQNYYIERVTIKGKDREDATVKFTNGLNIISGPSNTGKTSIVTA
                     IDFLYGAKKDAAPFSTDATGYSKVELIIQSTNGKYSLTREFDSNSILVSKITSSGEYV
                     DYEKYNARSGKNPISHFWLQLLGIDDDYQIIKNEDFKRQKLTWRTLAPSTLIPQDRVN
                     TSASVLFPGETTQRTAFLSSLLLLLYGSDFANSEENETKEHKKIRRNSVINYINSSLS
                     ELYENYEVAKSELPEAPSDDFQQIVDGMFAELETIEKQINDALASSKILIKEIYQKEQ
                     YLHETQLLLNRYKQLHYQYQSDIERLTLIVDGERLLNDHNEHDTSHSCPFCNGELSKP
                     VQTTYIDTARSELHSLLSQLSDLTEESHDTEVELQELMEQLDELKTRKKSLDAAINDE
                     LKPKAETLKDRINKIETYRDIKQQLISMEKMQNKLKSDLEKIENEPEDERILYKPKEK
                     FDAEFYKEMSELLDSMLRNMHYKPNEFRNAFFAKDIFDIKINGNLKWTENGHGYTAFI
                     NSVVVMAYRHLLYNHAKYTPSVFIIDSPLLGLDERKKQDGDDNMKEGLFKYFMDHQNE
                     GQLIIVENTTNLPALNYDDKQVNQIVFTEDKNNGRYGYLYDIYN"
     gene            42837..43040
                     /locus_tag="ACHGMT_000052"
     CDS             42837..43040
                     /locus_tag="ACHGMT_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6833834.1"
                     /translation="MKFSYNPLWKLLIDRGLNKAQLRKMTDISSATLSKLNKGENVTT
                     DILLRICEALDCDLSDIVEIVRD"
     gene            complement(43091..44227)
                     /locus_tag="ACHGMT_000053"
     CDS             complement(43091..44227)
                     /locus_tag="ACHGMT_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFN6833835.1"
                     /translation="MANSLDSIFEDDAFDDLTKQVEKKKVVKQDPEVAKFQEIIDFVN
                     ENGREPEKTPEWSSERALWARLSGFRGKPERSEKVKKYDTLGLLEKEFEPDGEFLADS
                     VEIDDELNLDDILGSDDMIDDFSDLLDVSRYRRTVNAADKIGRRKHASHFEKYKSLFD
                     QVHADIASGRRKIIPFEQYDIHENRFYIQNGVMMYIVSISKDTFIADNGKTNARMHVV
                     YENGTENKNLLLQSLASSLYSTERHGRMVTEPVDEETLAESFGEEFTTGYIYVLKSLS
                     TNPDISKWNNLYKIGFTRNDVESRIANAENDVTYLNAPVKVMLSAEVQNVNAQLLERT
                     LHHVFQDKQVVFQDGNFKKATEWYMVPLNDIESKINEVIAGLQK"
     gene            complement(44217..46172)
                     /locus_tag="ACHGMT_000054"
     CDS             complement(44217..46172)
                     /locus_tag="ACHGMT_000054"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFN6833836.1"
                     /translation="MTSNLFEINFDKNQSKTTNDLGMREMQERVYEKRASQYLLVKSP
                     PASGKSRALMFVGLDKLHNQGIKKVVIAVPERSIGKSFKDTELSKFGFYWDWEVKPSD
                     NLTIGGGEKSKVQHFVKFMNSTDPSDNILIATHATLRFAFEELDDSLFDNTLLAIDEF
                     HHVSRDDKSVLGNALRSIMSNSNAHILAMTGSYFRGDSVQILEPADEDKFDKVTYTYY
                     EQLEGYKYLKSFAMGYSFYRGKYTDALDKVIDVNKKSIIHIPNVNSGESTKDKYAEVD
                     EILDLLTDGGNITQNEDGIYEIERPDGKKLLVADLVNEEGREKVSAYLASITDDIKDL
                     DKLDIIIALGMAKEGFDWPFAEYALTIGYRNSLTEVIQIIGRVTRDSPNKTHAQFTNL
                     IAQPDAQDDEVLFAVNSIMKAISASLLMEQVLAPVYKFKPKDGKPDKPGDLSIRGLKP
                     ADTTERTKKIIENDMEDLKSSILQSQDIQNAIVSGADAKMINKTLIPRVIIDRYPDLT
                     NEELETVRQHTVADINIAAGRTTTSEDGSREIIKMADRFINIDELNIDLIDQVNPFQR
                     AYEVISRDIDAPTLRFIQDYLSSNKYDFTDEQLVAAYQAAKKFRAENGRNPDRNSKDD
                     GEKYLAFALLRLAEIKREREAAKEDGE"
     gene            complement(46165..48882)
                     /locus_tag="ACHGMT_000055"
     CDS             complement(46165..48882)
                     /locus_tag="ACHGMT_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HFN6833837.1"
                     /translation="MAKNKVLNITEIEDGVKKILSNRGDDFITQFLSLYDIPKTSITR
                     AKSKFDNGEPFVIKNKLHYEEIEGNVVIAIDAIAQSIKEQKSKPRYILVNDYLDIAAI
                     DTKTHETLNIPIEELPANADFFLAWNGIEKADYQAENPADRKAAERFAKLYDVVAKDN
                     PDANEHAFNLFLIRVLFLLFAEDTGIMNKGSFTNVLKTRTAEDGSNFNEVIKELFAIL
                     DINELARNGKDEWLLEFPYVNGKLFGEPHVDLTFSKVSRELLIEAGELLNWNEINPDI
                     LGAMIQSVASAEDRHVAGMHYTSVPNIMKVIKPLFLDDLTESFETLKLRSEENELKDI
                     TEKTRNDNKRTIINDLSALLTRISNIKFLDPASGSGNFLIIAYKEIRRLEIKILQLLG
                     DLQNNDSMPLSVIHLGNFNGIELDDFAHEVARLSLWIAEHQMNKELEEAIPGTIAELL
                     PLKDAGNIVCANSLRIDWNEVLRLKENHEIYIMGNPPYLGKSLQNAEQREDMEKLVGD
                     IRKYKLLDYIFGWFWKAKQYIATSHGSFAFVTTNSICQGEQVSILWPYLLKDEVRISF
                     AYQSFKWNNNAKNNAGVTVVIIGMTNNVNIHPKLYLENGSVMSVSNINAYLANGENVF
                     VNESRNSISNLPSLKFGSMANDGGNLVFSVAEYWNLIQKYPDIKHLFKKYLGSTGLIN
                     GDLRYTLWLTEEDATRYKQNSLIKETIKNVKSYREKSKRKETRALAEKPWSFGEVRYD
                     ETSENSILIPRVSSEKRDYVPMGIVGSDTIISDSAMAIYNAPMWLLGLLESRMHMVWL
                     RSVGGKLETRYRYSAGLVYNTFPIQDLSAQRKNEMTRVMTEILDLREYEGGTLADLYN
                     KDSMPESLRKKHQELDGIVDRAYQQRPFESDEDRLGTLLKLYQEMTIND"
     gene            complement(48883..49104)
                     /locus_tag="ACHGMT_000056"
     CDS             complement(48883..49104)
                     /locus_tag="ACHGMT_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6833838.1"
                     /translation="MSISYNKLWKLLIDLNMNKTQLREISGVSPNVIAKLGKNELVAM
                     ESLLKIAAALNVDVSEIISSDVIDAEDVK"
     gene            complement(49253..50089)
                     /locus_tag="ACHGMT_000057"
     CDS             complement(49253..50089)
                     /locus_tag="ACHGMT_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFN6833839.1"
                     /translation="MKQIFIFSDTQKDNISYFEREPAILVSMSRSDLPYLKEQDFSNY
                     PAVYVLIGGNKRYVGQAAGQSISLRLSQHFLKEYKAWVESVLFFTRADGKMSKADTDY
                     LEKRLIQDFQEKSDYEMMNSTAGNTSYIDKLQKAKSDQLYDTVFEIIDEIANIDLFGT
                     SEDSETQIYEFSSSGQYEIEYDGKKISSKSARGLLVDFVSQLFADGNYNNQLQELIVD
                     ETPSFAIILGRKPSMYNGRPNSARVADGVWVYANFNKKNVRIKIDKLARQLGIPVKIK
                     WN"
     gene            complement(50298..51674)
                     /gene="rlmD"
                     /locus_tag="ACHGMT_000058"
     CDS             complement(50298..51674)
                     /gene="rlmD"
                     /locus_tag="ACHGMT_000058"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFN6833840.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIECV
                     GLLTRVER"
     gene            complement(51785..52840)
                     /locus_tag="ACHGMT_000059"
     CDS             complement(51785..52840)
                     /locus_tag="ACHGMT_000059"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFN6833841.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(52850..54280)
                     /gene="gatB"
                     /locus_tag="ACHGMT_000060"
     CDS             complement(52850..54280)
                     /gene="gatB"
                     /locus_tag="ACHGMT_000060"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFN6833842.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(54277..55749)
                     /gene="gatA"
                     /locus_tag="ACHGMT_000061"
     CDS             complement(54277..55749)
                     /gene="gatA"
                     /locus_tag="ACHGMT_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFN6833843.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(55749..56054)
                     /gene="gatC"
                     /locus_tag="ACHGMT_000062"
     CDS             complement(55749..56054)
                     /gene="gatC"
                     /locus_tag="ACHGMT_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFN6833844.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(56160..58196)
                     /gene="ligA"
                     /locus_tag="ACHGMT_000063"
     CDS             complement(56160..58196)
                     /gene="ligA"
                     /locus_tag="ACHGMT_000063"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFN6833845.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(58213..60447)
                     /gene="pcrA"
                     /locus_tag="ACHGMT_000064"
     CDS             complement(58213..60447)
                     /gene="pcrA"
                     /locus_tag="ACHGMT_000064"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFN6833846.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            60602..62161
                     /locus_tag="ACHGMT_000065"
     CDS             60602..62161
                     /locus_tag="ACHGMT_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFN6833847.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(62272..64203)
                     /locus_tag="ACHGMT_000066"
     CDS             complement(62272..64203)
                     /locus_tag="ACHGMT_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFN6833848.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(64221..65135)
                     /gene="pfkB"
                     /locus_tag="ACHGMT_000067"
     CDS             complement(64221..65135)
                     /gene="pfkB"
                     /locus_tag="ACHGMT_000067"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFN6833849.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(65132..65884)
                     /locus_tag="ACHGMT_000068"
     CDS             complement(65132..65884)
                     /locus_tag="ACHGMT_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFN6833850.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(66043..66252)
                     /locus_tag="ACHGMT_000069"
     CDS             complement(66043..66252)
                     /locus_tag="ACHGMT_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6833851.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(66263..67591)
                     /locus_tag="ACHGMT_000070"
     CDS             complement(66263..67591)
                     /locus_tag="ACHGMT_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6833852.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            67727..67882
                     /locus_tag="ACHGMT_000071"
     CDS             67727..67882
                     /locus_tag="ACHGMT_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFN6833853.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(67956..68543)
                     /gene="yihA"
                     /locus_tag="ACHGMT_000072"
     CDS             complement(67956..68543)
                     /gene="yihA"
                     /locus_tag="ACHGMT_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFN6833854.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(68626..69876)
                     /gene="clpX"
                     /locus_tag="ACHGMT_000073"
     CDS             complement(68626..69876)
                     /gene="clpX"
                     /locus_tag="ACHGMT_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFN6833855.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(70123..71412)
                     /gene="tig"
                     /locus_tag="ACHGMT_000074"
     CDS             complement(70123..71412)
                     /gene="tig"
                     /locus_tag="ACHGMT_000074"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFN6833856.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(71566..72498)
                     /locus_tag="ACHGMT_000075"
     CDS             complement(71566..72498)
                     /locus_tag="ACHGMT_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6833857.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(72655..73314)
                     /locus_tag="ACHGMT_000076"
     CDS             complement(72655..73314)
                     /locus_tag="ACHGMT_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFN6833858.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(73317..74342)
                     /gene="sppA"
                     /locus_tag="ACHGMT_000077"
     CDS             complement(73317..74342)
                     /gene="sppA"
                     /locus_tag="ACHGMT_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFN6833859.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(74355..74645)
                     /locus_tag="ACHGMT_000078"
     CDS             complement(74355..74645)
                     /locus_tag="ACHGMT_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6833860.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            75055..76860
                     /gene="glmS"
                     /locus_tag="ACHGMT_000079"
     CDS             75055..76860
                     /gene="glmS"
                     /locus_tag="ACHGMT_000079"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFN6833861.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(76932..78287)
                     /gene="glmM"
                     /locus_tag="ACHGMT_000080"
     CDS             complement(76932..78287)
                     /gene="glmM"
                     /locus_tag="ACHGMT_000080"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFN6833862.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICARYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(78311..79480)
                     /locus_tag="ACHGMT_000081"
     CDS             complement(78311..79480)
                     /locus_tag="ACHGMT_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFN6833863.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(79477..80364)
                     /gene="cdaA"
                     /locus_tag="ACHGMT_000082"
     CDS             complement(79477..80364)
                     /gene="cdaA"
                     /locus_tag="ACHGMT_000082"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFN6833864.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(80553..84233)
                     /gene="nifJ"
                     /locus_tag="ACHGMT_000083"
     CDS             complement(80553..84233)
                     /gene="nifJ"
                     /locus_tag="ACHGMT_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFN6833865.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            84431..84952
                     /locus_tag="ACHGMT_000084"
     CDS             84431..84952
                     /locus_tag="ACHGMT_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFN6833866.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(85651..86216)
                     /locus_tag="ACHGMT_000085"
                     /pseudo
     CDS             complement(85651..86216)
                     /locus_tag="ACHGMT_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(86395..87051)
                     /locus_tag="ACHGMT_000086"
     CDS             complement(86395..87051)
                     /locus_tag="ACHGMT_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6833867.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(87619..88554)
                     /gene="whiA"
                     /locus_tag="ACHGMT_000087"
     CDS             complement(87619..88554)
                     /gene="whiA"
                     /locus_tag="ACHGMT_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFN6833868.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(88588..89586)
                     /gene="yvcK"
                     /locus_tag="ACHGMT_000088"
     CDS             complement(88588..89586)
                     /gene="yvcK"
                     /locus_tag="ACHGMT_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFN6833869.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(89583..90467)
                     /gene="rapZ"
                     /locus_tag="ACHGMT_000089"
     CDS             complement(89583..90467)
                     /gene="rapZ"
                     /locus_tag="ACHGMT_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFN6833870.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(90602..91333)
                     /locus_tag="ACHGMT_000090"
     CDS             complement(90602..91333)
                     /locus_tag="ACHGMT_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFN6833871.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(91337..92602)
                     /locus_tag="ACHGMT_000091"
     CDS             complement(91337..92602)
                     /locus_tag="ACHGMT_000091"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFN6833872.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(92865..95681)
                     /gene="uvrA"
                     /locus_tag="ACHGMT_000092"
     CDS             complement(92865..95681)
                     /gene="uvrA"
                     /locus_tag="ACHGMT_000092"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFN6833873.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(95691..97685)
                     /gene="uvrB"
                     /locus_tag="ACHGMT_000093"
     CDS             complement(95691..97685)
                     /gene="uvrB"
                     /locus_tag="ACHGMT_000093"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFN6833874.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            97944..99446
                     /locus_tag="ACHGMT_000094"
     CDS             97944..99446
                     /locus_tag="ACHGMT_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFN6833875.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            99448..100185
                     /locus_tag="ACHGMT_000095"
     CDS             99448..100185
                     /locus_tag="ACHGMT_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFN6833876.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
BASE COUNT        27721 a        22436 c        17894 g        32393 t
ORIGIN      
        1 cttctttttt ccaatttttc ttctgctcaa aaattaattc actgaatttg tcagaatctc
       61 ttgatacata ctgctcaaaa agcttttttg tgaattcttc actaaatcga atagcaaatt
      121 ctcccgaata cagttcgacc cactcagcat cgtttagcaa ggaacgaaaa ccagtcccct
      181 tatttccgat attttgtgat gatttattac tcgctcttcc taagcacaat ctttcaattg
      241 tatcttttga aaatgaagtg cctgtattcc caacttccaa gatattattt ttatatgtta
      301 tctgaacttt cacttcgtca ttgctagcat cattttctga ttttatatct ccataggcat
      361 cgtctacatt ctgtaataac tctaaaatct gacgcccatt ataatcacgc atcaattcat
      421 attcttcgct gcaggccttt tcaagttcat tactcaactc agaatccgta tatttacgaa
      481 taaaaatatc ccttaattca tcaatggtat tccattggct cacagaactt cgcccccagc
      541 ctatttttta cataatatat tatatcatca atcactttat gaaatccaat caataaagac
      601 gttaatatgg ctacccacat taacgtctag tcctatctat tcaaattaaa tttctacagt
      661 cactctcacc ccacttttga aatcaacaac aatgttcttt tcaaaaatgg tgaccttctc
      721 aatcaacctt cgaacaagca cttcattata ctcattgatg acatcgtgtt cttttacaaa
      781 gactaccatc tcatcaaccc gttgttgcaa attagtattc gctgctattt gttttgcaac
      841 cgcatctcgt tcatctctta gtgaaaatat ttggcttgcc aattcatcgt aaccctgacc
      901 gccaactgcc aggctaataa tttcagcttc aagctctttc aactgactgt ctagttctgc
      961 caatcggtca gttaatccat cagtcataac tgagttgata ttgtcattta attgctgaag
     1021 aaaagcatca cgatcgatta acgtttcatt gattgccttg ataacagctc catgcaggtc
     1081 tgtttcgtag attgttcgac ccgtgcaatt atgttctcgt ttatccactc tggatttgca
     1141 tcgccacaca gttcctcgat tcttccaatg aactctgcgg taagcattgc cacagtcacc
     1201 gcaaactgtg ataccagata acgcatactt cccagaaaag cactgtgact ttttgttttt
     1261 cattgattta cgccggtcta gttcttgctg ggcttgcatg aagatatctt ttgggataat
     1321 ggccggatgg ctatcttcaa cataatactg ctgagcgtaa ctcccgtcat tcttcactcg
     1381 tttctttgtc aagaaatcgg tagtatatgt tttttgaagc agcgcatccc ccatgtactt
     1441 ctcgtttcga agaatcaatc tgactccttc tcctcgccag agctttcgct tccctcccgt
     1501 taggatgcca tcagccatta gaccattagc aatttcttgc aaggaactgc cctcaaggta
     1561 ttcgcggaag atgcgtttga tgacttctgc ttcctttggt tcaatcacca agttaccatc
     1621 ttcgtcctta gtgtacccaa gaaaatgatt gtggttgata cgtagctgtc cctgctggaa
     1681 acgatactgt acgcccatct tggtgttttg acttagtgac tggctttctt gctgggccag
     1741 acttgccata atggtcaaaa gtacctcacc cttcgcatcc attgtattga tgttttcctt
     1801 ttcaaaaaac actgcaatct tcagttcttt taactgtcgt atgtattgca agcaatcaac
     1861 cgtattacgg gcaaagcgtg aaattgattt tgtgatgatg tactcaactt cgccgttttg
     1921 acacgcctca atcatccggt taaaaccgtc acgctttttc gtcccagttc ctgaaatccc
     1981 ttcatccgca aatattccta caagctccca atcgggattt ctttgaatgt agtccgtata
     2041 gtgttgaacc tgtgcttcgt aagaactcgc ctgttcctcc atttcagtag aaacacgaca
     2101 ataggctgcc actttcaact ttggtttctc ttcggtattc tctgctgccc attgtctagg
     2161 cttcattgga ataaccgtca cctgttttcc tgccatttca cttaacctcc ttgatttttg
     2221 aatatgcata ttgcgcttgt ttgaatgggt cagaatactt ttcggtaatc ttgccagatt
     2281 taaaatgagt gggtattacc ttcgtatctg gcactttgat ctccttgcgt tttttaaaga
     2341 acttaattct tcgttcttgc tccagatccg cttggaagaa aatatcctca tcaatgattt
     2401 ttggatatat ctcatttcct aagtaaacct cattcatcaa aatacgtttc ataaagggat
     2461 gcggcttgtt aatgccagag cgtttgccag cttcttgtaa gccaacaccc tcaaggtaaa
     2521 tattgaagaa ctcacgtatt tgttgagctt gctgttcatc cacgttgatt tctccattct
     2581 taatgacata cccgtatgga attgtattgt tcattctggt aacctctctt tcaaaagtag
     2641 tccgcactta agttcaaatc cgacttcttg gcggttgtaa actgtaatgc tgtttgtgaa
     2701 cttcttgaat aaatcttcat cccagtccgt caacatctct gccatccgcg cgaatttgta
     2761 taaggcttct agttcaattt gctgagtgct tttgccgttg actgactcaa tgatgaggcg
     2821 ctgcttttct tgtgcgacag ccctctcttg ttgaagtttg ctttgttctt ctaaagacac
     2881 cttcgttgat aaaatactgg aagcagtcaa acttgccaga acctcaagct tttcttgaat
     2941 cttgtcgatt tcatcagcaa gcacttcgag cccttcctga ttcgcacctg ctttattcaa
     3001 agacttcata aaaggttcca gaaccaactt gtgtccgaaa atcaacttat tcaacatggt
     3061 caaataagca accttcaaat cttcatccgt aattggtaat gcatcgcagc tatctttatt
     3121 tttaagatgc cccgtacact tccactgaac cttcttcgta ttacctttcc aataagtgtg
     3181 aacccgcttg aatttgctgc cgcatttatc acagatgatg ttgcctgaaa atgcgtatcg
     3241 cttggtgtac tttttcccgt caccagtatt ccctttggcc tcagcttttc tccgaatgag
     3301 ttcttgaact cggtcgtaat cttctttaga tactatgggt tcgtgattgt ttttgatctg
     3361 gtacattggc aattcgccct tgtttatgcg tcgtttgtac tggtcatcaa caaaggtttg
     3421 ttgcatcgtc aaagtcccgg tataaacttc atttttcaaa atacttctga ctgatgttgc
     3481 ttgccaattt gccccacgtt tagtgaattg ttttccatcg tttaaccgct tggcaatcat
     3541 tccagtcgaa atgccatcta atgcccactg gaacatttgt ttaacaactt ttgcttcagc
     3601 tgcaacgatg tgaaaatcat agcctttccg cttgtagcca tacggcgggt aagctttgaa
     3661 tttcccttta gacatccggt ttcgaatcgc ccacttcaag ttttctgaat gggaatagga
     3721 ttcactttct gccaagcttg ataaaattga aagcaacaac tcgctttcca tatcaccagt
     3781 attaatgttt tccttttcaa aaaagatggt gacaccaata tcagttagct gccgaaccag
     3841 attcaagcag tcaactgtat ttcttgaaaa tctggaaata gactttgtca gaatcaaatc
     3901 aatctttcca agctctgcat ccttaatcaa tcgttttaaa ccatcacgtt ttacagtctt
     3961 ggttccagaa atcccttcat cataataaac accgacatag tcccattgtt catggttctt
     4021 gatgtgattt tcatagtgag taatctgggt ttgtaaactt gccagctgtt cattacttga
     4081 tgttgataca cgagcgtatg ctgccacctt tagtttggaa gcttccggca actctggacg
     4141 tgccaattta tgtatcgttt tcatcagctc aactcctttc cattaggtga catgttagct
     4201 ctaaagtgcc gttatatcaa cgtttcagac cattatcctt gataaaaaag gattgaatgt
     4261 ttcccggttt ttattgtcga ttttgacgta ttcttccagg ttgattttac cggtaacaag
     4321 taagttcaga aggatctgct gtgaaatgta ataggctgct tcatctgaat agttaatttc
     4381 tgttttcttg ttagtcattg ctttcgcctc ctcaagatac aggcgaagaa aatccatgaa
     4441 agtaaaccca atcacaaatt ttcctttgcc tttcatccat atgcgaaaga aataaccgac
     4501 tcgaacccac aaaacgaaaa aagcctgcaa gaatgaaacg aatcactctt gcaggcttct
     4561 aagtttgttt atatatagta ggaattattc tttgtaaatt ttgacttttt acttcccaaa
     4621 catcaaataa gcctttacaa gcttcatcca ataaataaca atataaatat caattgtggg
     4681 aaggagaaag ttatgagtag ttttgatatg gaagggttag aaaaagaata tgaacatttt
     4741 tgcaaatctg cgacaacaaa ggaactggag gaatttgatg aaagcttcaa acaagaattt
     4801 gaaaagtgct ttaaaaccat agaggctgaa gaagctttat accagttttt acaagccaat
     4861 ataaacgcga tgaataatcg tatggaatca atagaatcgc ctcttttctg catcaattac
     4921 aaagagcagg tatttcagtt aagaaatcat ttaatcctaa tgcaaaatgc tgtaaacacc
     4981 taccttaaag aaaactagac gaaaaaaagg ccccacaata aaacttgtga ggctgttttg
     5041 aattataaag acgtcaaact acttattgaa aattttgtag ctcgttggaa ccatcgtgaa
     5101 gttttcttca gtaattgggc catcataaat tttgattttg caaacttttg acgccacatc
     5161 aaggtcgtga acaaagtaaa ttttatatgg tgtatctcca agggaggcag caacctcatt
     5221 agtactcatt tcaaatccgt cagagaagtt tgtttttgaa gctttcactt caatatggat
     5281 ttcattacct gattggtcaa acgcacaaat atcatagcca tgaccatctc cttcagtttt
     5341 agaaacatgt tcaggaagtt ttgttttgtt cttttctgct tcctgaatga ggaaatcaag
     5401 tacgatttct tcgcctagag cacccgtttt ttgttttgcc ttgttgacac gctcccaatc
     5461 agtatggcgt ccagtgaatt ttcgtttctg agtcccattc gtgcgatttg gtcccacttc
     5521 atcaacaaga acagtaaatc cttttgaact ttcttgctca atttcttcaa cttcctcgtt
     5581 tgtaagatcc agttcagacg gcaacgattg tgtatcatcg attgggataa gatgacgatt
     5641 ttcaatcaat gcaacaaaat cggcataggt aatttcattc attccatact gatcccagaa
     5701 gtaggcccat gaattcggct ttttacgctt ctttttgcta ggtgtattcc aatcgaagtt
     5761 ttcaataaat gaatcacctt gtttaattgc cggttcgatt gtaaatgcgt gtgtaattac
     5821 tgccgtaaca tttccagctt catcaggttc agaaatgctc tcaacgtatc cgcccccgta
     5881 aatttcccac ttacggtctt tagtaatttt ccccggatga cgattcaaaa caaccgcgcc
     5941 ggctttcaat ttaccatatt ggcgcatgtt ccaaccgtaa cttttagcat catcagcgta
     6001 tttcaactga ccatcatctt ctgttttccc gtgatctaaa atgaaagctt gttggctttc
     6061 tgttggttct ggtaacaaat ctttaaacgt aaccatatcg atacctcttt ctataattca
     6121 atcggaacaa atttatgttc tggttgaatt aataataaca cgttcgatat tttaagtcaa
     6181 caatccggac ataattttgt tccgattaaa tttatgaaat caaaatataa tcgtcagatt
     6241 ccgtcttacc agtattctta ccgacagcac aatatccaat cgggcaacga gcccataccc
     6301 aaccatccgc aatcgtcatc ttctctgact tcggaagcgt ccaagttgcg cctttattgt
     6361 atgaaccaac aaccgcacca cttaaacttg gcttgttgcg aacgttgaga ccactcactt
     6421 gaaccacaag agttttcacg ttggatgttg atgacccaga tggataaact tgattaccgt
     6481 tctggtcgaa caccttataa ccagaatggc tatctgcagc ccgcttcgca ttgttcaagt
     6541 ccttataggc accaatttgt gacttggcgt cagcccatga tttgcgaaca cgatacagac
     6601 cactagctgg cgtgcttggg gttgatgaac cattattaat cgcattggct ttatccgcaa
     6661 tctcctgcag tttaccaagc aactgtcccg gacatgatgt ggccgaaaaa tgtgagtgag
     6721 ggaagatatt cttgttcacc accaatttcc ctagaccata acgtttggca atatctgcac
     6781 acaactttgc acttgcatca atagttgctt gattgatacc ccagttcggt gatccagtgc
     6841 tattgcagtg ctcaatcccg atacttgaga tattggcgtt ccaattacca gcgtgccaag
     6901 ctgtgtttcc ttcgtccacg tatgctcgaa tttctccatt ttttccaata ccgtagtgtg
     6961 ctgacgcctc acgcgttttc caaatatcag gaacaatatc atagttggtg gttgccatgt
     7021 gatgaatcac tatcttatct actttggcac cactacgacc attcgagtaa tggctaaatg
     7081 actgaaatcc atattttgtt gctagattac taattcccat tttctgtttc ctccttcttt
     7141 tcgtgtaact gctgcaagat ttcacgcaac ttttccggca ctggcagtcc aatcgccacg
     7201 gcattctcca gcagtgaaat tccttcgttt gagatataga agaaaatgac cgcatcacgc
     7261 agtacatttc cacctttgat gacttccaca tcaagcatat gagccacacc aaccaaaaga
     7321 aaaatcatga ttttcttagt gatgcccttt gccccaatct tgctggatag tttcttttcg
     7381 ttgattgcac gaagcagacc agttacatag tccactacca caaaagttac aagcgtataa
     7441 atcaaaccat caattcctcc cacataccat ccaatcactc ctccaccacc tgcgatggcg
     7501 agttgaatcc aattccacac ttctttcatt tacaaatcct ccaattgttc taaaattaaa
     7561 tcgagcattt cagcatcttt ccctgaaatg gtgacttcac tattttcaaa tccagcaacc
     7621 aataaaggca gcttgtcttg aagttcatcc acgttgatgt gggtttcttc gttgagcaag
     7681 tctgcctttt ctttgtgata ttcagttgcg gtttctggga tgagtgaaaa ggcaccgtca
     7741 tgttggacca aattgccgtg ctcatctttc tttccgtatt tttgaagcaa gtctacctcg
     7801 tcttgataga gttcatcaac cttcacctgg atgagttttg caagcttcgt tcgcactcgg
     7861 ctaatctttg gtggtaagtc aaaacctttt aagaaactca tcacgtccac caaatcttca
     7921 tttctaattt tgagcattat ctatcttctc ctttaaatca ccaatctgga cacaaagatc
     7981 atccacgacc atcacaagca aatcaaccat atccgtccca actttcattg gctttggaat
     8041 atctgcctcg ataggttcag ttactatcac ttcttctttt tcttcgttca tggcggcctc
     8101 ctaactgtat gtggtcattg aattaaacga ctgcggattg taccaaccac tcgctgtacc
     8161 gttgctactg aaaccggttg gtaaagcgat tgttcgtccc gcaagtttgg cacagatttc
     8221 acggatgact ccaaagtcaa cccacttccc atcatctccg agcatcagat tagaaccgtt
     8281 gaatgcaata ccagcgctgc cggaacttga gccaatctta gtataaaggg aactactaaa
     8341 tgtaatccca ccaacagata tttgatgttg tgtccctata cgaagcattg atggtaaata
     8401 aatgtcgtcg tcaaagtgga accccttctc cataccagac tgcaatgttc tcgcatacca
     8461 agaaagcttc tgggtgtaga cactcgcaga ggaggaatct ttatgagccc atgacatata
     8521 ctctgttcca tactcaagat caaataccaa tccacgccaa tcagagtgac cactgatgtt
     8581 atttgcacca attgttccga ctgtcgttcc accacgtcga atggtcattc cctgcgagtt
     8641 tatttcagtc acaagagtgc ttcctgaata atatccaatc gtagttcctg tgattcgaat
     8701 ggacgaactc gcagtcaatg ctgttaggat attagcagca atcttatcag ccgtaatcga
     8761 gttcgcgcca atacgggctg ccgacaaagt tcctgtggta atcttcgccg catcaatgct
     8821 tgcaattttt gcactttgaa ttgtggcatc tgcaatcttt gcgtttgtga tggccgcatt
     8881 ttggatcatg gcagaactaa tcactgcact atcaatggtt gtttgaccag tgatgtgcac
     8941 cttgtttcca gcaatcaaaa tactttcagg tgagatattg atttgattaa tcacgtctcc
     9001 tttaccaact cgtaaattga ttaagtcttt ctgcatgaga attgctgaat ataatgtgtt
     9061 ggcattggta tagtcttgcc ttgtggtcgt aggataaata tttccttcat attcatcggg
     9121 tttgatgctc gtgttcactc ggactcgcat tttcaatgtg ccactcggga ctgtaattgc
     9181 tccgctcgaa ccagtataag agatttgatg ataacttgaa ccatccgacg ggtttagagt
     9241 aaaaggtgtg ttgcttgaat tataaatccc agcatatgtg ccagtcttaa ctgaaaagaa
     9301 agctagatac acgctgtcag tagtaatccc tgatatctgc tcagccgtta acgtccaaga
     9361 ataagtggca ttgctgctac ttgtaacggt cgaaatctgt tcccaagcac cacctgtata
     9421 aagcaagaca atattatctg atgtatcctt gcgaccattc cacgaaatta cactcggagc
     9481 gactttgcct tccaatggca atttgtaaat gaccagataa tctcctgaat ttgaataggg
     9541 gtttgttgtc acgccacttg taatactcat cgattcaacg ttcttactta ctagataact
     9601 cgcaatggtc gcattagtat aagttcccgc cgttagggtt tcactaaatt gttttgccgt
     9661 tgggacaaaa tcaatgtaag gtaaatccat gccgtagtaa tgatagtaga aagcagcttg
     9721 agttccgtca cgcagttgcc cgatatactt atctcccact ttgacatcaa ggaactcact
     9781 tcggagataa ttggttgcgg acacttctga accgtcagtg gtactcaaac ttccttgttc
     9841 ccattgattg actttaaatg gaacgccgat tgcttctttg gtttctgaaa cacttaacag
     9901 taaggcatca gtggtcgctg taagttttac gacgttactt atctcggata aatcaacata
     9961 gccatttgtc acatccgttt tcaacatata gcaactgatg gtttctggtg catcactagt
    10021 tgttaaacga atgcgaataa aagcggcact gcttggaaca gtaagcggtg tggtggtgtt
    10081 tccaaattgg gtgtacgata aaaaggactt atcctctttg taataaagca gataagccgt
    10141 tgcgtaagta gagtaaagtt tcccacccac aaatgtttga agtagatact tttcgccatt
    10201 tctaactctt gtaaaacctg agcgaacgta tccaccatag gtattttctt ttccatcatt
    10261 tgtattcaga ccaccaactg cccactgcaa atctgcaatg ctcataccgg ctaaaacatt
    10321 gaccgcttta tttgttttag aaagagatag ctcaaatcta tcagcatttt gggtgatggt
    10381 gctttctgca gttgtaatac gccctgtcag attatcaacc gtcgattgac ttgccttact
    10441 agcaatctgc ccagcttgca ctgtcagagt tgattcagct gtgattactc gtccttcaag
    10501 agttgacata ccactcgcca ctaatgaaat cttgctagta ttctgttcaa cggatgtagt
    10561 aagcgtttca acacttgcac ctacactttc agctgcattc aaagcattca cagcatcgtt
    10621 aaatgcttga ttggcctttt cagtggcagt attggcatcc ttttgtgctt gttccacggc
    10681 ttgctttact tcttctgccg tcatatctgt cataagtgga taccactgag tgacgccatc
    10741 acggatttca tacacccaca tttcaaactt gtcaccattt tctttgtacc aaacgtcacc
    10801 gagtttcgga ttatttggag ttgtcgggcc atagaagttg gtgtttttac catttgccga
    10861 actcaatgcg taattggcat cgtctttggc ttgctccgcc ttgttgtcca cccggttgat
    10921 gtccttcgcc acatccgtga attttggcgt gatgtttccg agtgttactg agatatacgc
    10981 tttcagaaat ggatcgtatt tgtatgagac cattctagct gtgatgttca atccatcttc
    11041 ggcatgaata actgaaacag tatcacccaa attaatcgtt tcaagctgtg caaaatcttt
    11101 atattcttca gttcgctcaa gcggggcaaa ctccacatca taggtggctt ttggcaaatc
    11161 gaccttgtta gctgagtatt ctaacccagc caatctgcga agttcatcat aggcctcggc
    11221 ttgagtaaac tcatcctctt catcaccaac tttgacgttg tcgtacttga tgactttgat
    11281 tttaggctgg acgtattggt tgatgtttgg actgtccacg tatttttcgg gaagaaatag
    11341 tccatcatac ccttgtggca taatgcgagt aacaattcca gagtaatcaa catcagcttt
    11401 atatcctgtc agattcttct tgtctcggat ttgtacgccg ttgttactgc cacgagtcgt
    11461 taacattgaa atcgtgaagt tatcgcgtac aatttcacca ccataactgc tttgaaaacc
    11521 attctctagg tcactatcaa gcaacacctc tacaggattt agtcgcacca agcggacatt
    11581 attgaccgca ctgatgtttg atgtaccggt aaaacggtgc atgaattgag ttgcaccaag
    11641 cagttgtgtc aaagctcccg acccgttctt gttgacgata taagtatctt caataaagtt
    11701 tgtggctagg tcatagaaaa tatgatgagc tactatgtgc aaaaaaccac caagtgcatc
    11761 atctcgttca acaatcctga aaagctggtc ggacagttct ggtactggtg ctttcacaat
    11821 catttctggc aacaattcct cactgtgtgg agcatgaatt ggataatcga attcaaagga
    11881 aaagagtccg ttcaattcct cactcaccac ggggttcaga atgttgccat caaggacagc
    11941 gagaccgtta tgattgaaat ttgattctgt tttgttgtaa agcgtaatca taagtatcgc
    12001 cacctcggtt ctatttctac tttggttata cctgtaccaa gagtcacagt attactgccg
    12061 actcgaaact caggaaagtc acctgtcatg tattgattgg ctgccacatc cccgtaaaag
    12121 cattctttca aatccgaatc tagtgtcagg ttgccattcg tcagatttaa cacgataggc
    12181 ttgccattta tcgtgagtgt cctagttcca ttgccgtaga ccttaatctt cggcaatgag
    12241 taaatgttgc cgggattatt gaccgttcct gacgtggttc gattgatagt cgctacattg
    12301 gtcttataac gaaaaggcga acaggttaac tccatttcaa actcccacat ggaagataaa
    12361 atctgagtta gtccgctcgc cttagcatac ttgattgggt aaaacacgtt tggttcattt
    12421 gaaaatgcga tagttttagc gtttaggatt tgtggcaaaa ttatctgcca gctagtccaa
    12481 acatcagatt tccaaatcac taccttaaaa ccgaaagtca catctttcca gcctttcaga
    12541 atggtcaaat ctccttcacg tccatcaatc tcaatactat caatgacacg ttccgtcact
    12601 ggaataactg gcacttccgt aattcgcaaa cctaaatcag tccgtgaatt aattgttccg
    12661 tctaaaataa atgcgttcaa ttgaccaccg cccatctaaa taactaaaaa aggaccagac
    12721 aacaatgtct aatcctaaag tttataatct atcatcctat tcggttgttt cttccagccc
    12781 tattttcttg gtaatcgcta aatacgcgta tacaaccagc acgccggaaa ttccatatat
    12841 tgcatcgatt agatctggtg tattcagaaa agtcatcatc gtttcacata caatatttgc
    12901 aacaatcaga ccagctccca tcgcagtaag aacccttaaa ttcgttccca taagcaacca
    12961 aagatagcct atcaccaaat agaaaagaaa gcttaatgcg aagtttgaaa tgtgagaatt
    13021 aatctgattg ttgatgaata caaaaccatg atttatgccg ctataccatc ttactgccgt
    13081 taaaaataat ccaatagcaa gcagtagaat atatcctatc agtgcaggtt tctttgcttt
    13141 aaatttatgc ttcaacatat gattaccacc atctcttcag taaacactcg ttagttatag
    13201 tatacagtca aaatgtgaaa atccaacact aatatgctac acgtctacga ttcaacgtcg
    13261 ccaagctctt atcaatttct ggagcaagtt tgccgaccaa tgttccatca ttcaagacga
    13321 ctttaatgtc cattgcttta agtagcgttg gcaacatttc actcgtgatg tttgttaagg
    13381 catcaacctt agcacctaaa tcaagcatcg agaatgtgct atcaacggca gcgtggatat
    13441 cggtatcaat atcaaagctt ggcatataaa gttttgttgg aatagctttc tgcatcgttt
    13501 tagaaacacc agtcatttcc ttatcaaaac ctacgccaat cccttgtgcc atgtacttcc
    13561 cgatttcatc acgaaacagt gttgatggtg aatgaatacc aaagaagtcc ttgatgttgt
    13621 ctactacacc gccaaagaat cctgaaatct tgttgcgtaa ccaacttcca gcatcagcga
    13681 taccattcca caatcctgta atcaagttct tcccgatagt tcccatagtt gaaacatact
    13741 caccgaaagc attcgcaagc cctgtgatga tttgcggtac tgctttgaca atggcagaaa
    13801 tgattgctgg taagttttga actaatgcca caagcaactt cacaccagca ttgatgattt
    13861 tgtcgatgtt gtttaccaaa gttcctgtta ttccgttgat aatttgcggc atggcattcg
    13921 caatcgttgt gataatttgc ggtaacgcac caatcaatgc cacaagtaac tgaatacctg
    13981 cgtcaatcaa ctgagggatt gccccaatca cggcattgat gatattttga ataatcactg
    14041 gaatagctgc aacaatcgca ttgatgatat ccggcaaagc tgtcacaagt gatgtcaaaa
    14101 gttgaatacc agtctgaatg atttgtggaa tagcactgat aataaagttc acgattcctg
    14161 taataatctg tggcagactt tcaatcagta ttggaagtgc tgtgataagt ccttgggcaa
    14221 gtcccatgat aagttgtaaa gcagcatcca acagtaatgg caagttttca atcaagccgt
    14281 tcacaatcgt tatgattgcc tgtactgtaa ttggaataag ttctggcagc gcttcgccta
    14341 gtccttggat aagtgttgtc acaagttgaa aggcagcgtc aatcagtaaa ggtaagttct
    14401 caataagtgt ttgaacaatc gttaaaacgg cttgaaccac cactggaata agtgtgggaa
    14461 gtaagccaag tatcgtttca agcacttgtg agaataatga ggttactgct tctaagagtg
    14521 ttggcaataa ctgccccaat gcttgaataa tggcatccac cactgccggt aaagccgaca
    14581 cgatattttg aatgaccggt agaatattct tgatgacgtt ttgaaattgt tccaccacat
    14641 tgccaatcaa cataccaatg tcagcgctgc tgttaccaag tccagccatc aagtttgaga
    14701 ttgcagactt cataccgtca atcgaaccac ttatcgtctc actcgcttcc ttggcggttg
    14761 taccagtgat gcccatttcg gtttgaataa cgtgaatcgc ttcagtcaca tcagcgaagt
    14821 tcgagatatc atatttgaca ccagaaatct tctcagcctc ttggagcaaa cgttccattt
    14881 cggacttcgt gcccccaaaa cctaatttca agttgtccag catcccgtaa ttttgcttgg
    14941 cgaaaccttg ataggcgttc tggatggact caatatccgt ccccatcttg tttgcattat
    15001 cgctcatatc agtaacagct tgatccgcaa ctcctgcggc tttcttggta tcaccaccga
    15061 gagatgaaat caaggcagct gagaaacctg tgacagtttc catgtattca ttggctgaca
    15121 ttccggcagt tttgaaagca ttgtcggcat tcttttgaac ggtggatgac gcatccttga
    15181 aaagagtatc caccccaccg actaattgtt catagtccgc gtaagcagat accacttgtt
    15241 ttcctagctc cacggcagca gcgccagcag caagtgccac tgtacccatt gctaccccga
    15301 taccttttag aacgccaccg aacttttcaa atttacttcc agacttttca gcctcgtcac
    15361 ctgtatcctt gagttcgttg ccaagctcat cagttttctt agcagcatca acttcttcag
    15421 tacccatggc gtcaattgcc ttctcgttgt tagcaacttc acgctccata ttattgagtt
    15481 gggcttttgc attgtttaat tgcactgtcc aattctgagt acgcttatcg ttttcaccaa
    15541 aactgtcact agcgttcttc aaagcatttt caagggtggt gattttttct ttctgggcat
    15601 caatcgattt gtttaagact tggttgcgag cagacagtga gctgatgctt ttatcgtttt
    15661 tatcaaattc ggatgatacc agtttcattt ctgaacctag caccttgaat gattggttga
    15721 tttcacgcaa cgagttctta aactcctttt caccttcaac gccaatcttt aaaccaaaat
    15781 tatctgccaa ataaatcacc accttccctt aaattccact cggtatcaca tcatcaatga
    15841 ataactcaac tttcggcttg ctaatcccag taaattgctt atgacactcc cataaatcca
    15901 aaaacagtcc aagcgagcag taccagaaat catctgacgt catacccatt tgaactgtcc
    15961 catagtaata caggcgagta aatgtttctt catcacttac tcgcctgctt tgttttttga
    16021 atctgtttca ctttcgatat tgcgagctgt gcccttgaac attgcttcag tgattgctga
    16081 tttatattca gccaactgca acggactcgt catcaactcc acatattcag tcgtgagttc
    16141 atctttcttt tctgtgttct tcaagttgta aatcttgatg gactgattcg ccaacagcgt
    16201 aatcaaccag atgatttcat caagcgccaa ttcaaagttc tcggatttca acaatttctc
    16261 accaaggtta tcaagcccac cgtagtgact ggcgatttct ttcgtggctt ttgttgtcaa
    16321 aatcagttca tattcatctc cgccaaggct aatcttggca ctgcgttctt cactcattgt
    16381 tgcctcctag tctgctgtga aatctggttc ataaacactc gtgtaccatc cattgatagt
    16441 tgtttgttca actcccgtat cgttttcatc aacttctgct ttccatggat gcttaccact
    16501 agcatctaac ttgttacgac gcatgactgt accctcaatt gttggtgttg agaatgtaat
    16561 gtcgtcacct tttgttgcta ggcttgtgct gggcacggca aacttcactc ggtacaacca
    16621 caaatatttg tatttgccat tcgatttacg agcacggaaa ccaatcgcaa ctggtgtgcc
    16681 accatcttcg gttgtcgaaa ttaagacacc gttttgatcc acggttgcac caacaagcac
    16741 tgccgccaca gcacgtccaa tatcatcaac accgagcgtt aactttccat ttttaaattc
    16801 tttcactact tctgatgcac catcatcggc gtataaagtt gcttcagcaa gttccaccga
    16861 aagctcagct gagattgctt tcgcaagcat agcgggtgtg ccgtatgttt cttcgccagt
    16921 agttgccgac tctgtaatcg gtgcataata taatttatcc aatccaatcg ttgccatttt
    16981 cagacctcca tttcataatg cttggcgaca tcaatcgcca catgatagta atttgtatct
    17041 ttttcaaatc ctacaaataa tcgttcagta atcgtaaact cgtgtttcaa caagttctga
    17101 accagttgtt tttgcgtgtt cagatagttg cctttcacaa acaaggaaat gcgaacttcc
    17161 tgtacatcaa cggttggaaa gttatccccg taaacatcga acctgtcagc gagtggtgtc
    17221 agcacacaat acttgtctgg cgctttatca ctaaactcgc ccgtttcaac aggaatatga
    17281 agtgtggcaa aaagactgtt caattcctct aaaatgttca aatgccgttt acctcacttt
    17341 ccatttttga tttcattgtt tcaatgcatg acttgcgact ttgtgatttt gcaggtttca
    17401 agaatggctt tgctggctga ccgtgttttc catattcgag aatgttcgca atcttagcat
    17461 ttgaactgcc atcgattcga ggctcatcaa aaccaacctt gatgttccag ttgccgcttt
    17521 tatcttgacg agcttgtgtg ataccaaggg ctgattcaag ttcaccagtt gaacgacttg
    17581 gtacttttgt atttgagcca acaacggcag ttaagttact tcggactttg ccttccacca
    17641 cttcagcacc actttctaaa accttgggga tgatttcatc tgtcttgttt tctagttttg
    17701 aaacctttaa caagaaatct tctggcatct tcatcaatgc tttagccatc cgcactcacc
    17761 tccatttcag tagtcagcat ttcaaggtac atattgcgat ttcggacatt ctccaccgac
    17821 acaatctgat accttttctg atctgaaatg acaaccatct ctgtcgtaac ctctagatta
    17881 ggaatataac gaatccgaaa caggcaggtc gctgaactaa aagtcgccat gtttacccat
    17941 ttctcagtcg cattcttttc ctctttgtat gcatggacag ctgcaagaac ttcatcttgt
    18001 ttcactgaaa atccagcgct atccttttgg ttcgtcactt taacgatttg tattcgttga
    18061 ttcatctttc caaaactcat acttgccacc gcctatcgag cctgagtaac agattgaccg
    18121 tttcccagac ttgcttactg gcattgacgt tatccgcaaa gaagccacca gttgaaccat
    18181 cacggctttc gtaaaagtta ctggctaaca caataaccgc ctgttcagtg gttggcggca
    18241 tttcatgttc tgaatagtag ccagactcaa tatgttgata gctctcagca taagaaagag
    18301 cggtcagaat caatcgttcc aacagctcat catcttcgtt atgctcaagt atgaggtttg
    18361 ccttgacctt atttagtaag tcactcatag gctactcctt aagatgaggc agatgctttc
    18421 atcttcaaga tttgcactgc ttcaggtaac acaagtttac cgtccacacg ttctttagct
    18481 aagaaaccaa ccatgccatt acctgcaaag agttcacgca actggtcgaa tgaacgcacg
    18541 ccacggtcgc caatgttata gtagctgaaa tcaccaaaag caatgactgg cgcacctgcc
    18601 ttaacggttg gaacaaactc cgaagtataa actggataac cgagcagacg gtcaggttca
    18661 cctgctacca aagctggctg ccacatgtag gcaccattgt tgtctttcaa cttacgaagc
    18721 aagctgatgg tttggtcgtt catgataaac ttcgcgtttt tacgatatgg acgtttcaaa
    18781 gcatacacaa ggttcaccac atcatctgcc gtaactgcac tttgagtatt agttgtaatg
    18841 gcaacttgtc cgccacctgt tgctgcaaaa ataccgagtg gtttaccaac accgtcacca
    18901 ttcaagaacg catcttcttc cgcgtttgct aaggctttgg tgaattggtc gatgatatag
    18961 ttttcaaggt taaaggcgtt gtcataaagc aattcatcag tcaccttgat tgccacatga
    19021 agtttgtgag catcaagcaa gatttgatcg aacgttgcat ccccaaagct caactcgcca
    19081 ccttcttcaa tccatgaagc ggctggttta gaacctgcaa tattaatctt gtgttcacca
    19141 cttgtcgtaa ttgttgtcgc aagtgtacgc atgatgtttt cagaagtcat gccgtcaatc
    19201 aaacgtgagt catattcttc tggcaccaaa taaccgcctg ccgtatccac accttcttga
    19261 agaacatttg aaacatgtcg gaagtttgag cgcaaggctt caagtacacc ttctttatat
    19321 tcagctgaag cacgtcctgt tttcttaggt tccattttat tttcagtagt cataggttgc
    19381 gaagttaatg gttggctcat tggcttttca agttcacgat ccatcgcttc cattgactgc
    19441 atacgttcaa tttccatcgt aaagttcttc acttttgttt ccatttccgc ataagtcttg
    19501 gcatcttctt cagacatcaa gccatcttta tcttgctttg cttctacaaa agcctttgct
    19561 ccgttccaag cttgtgcacg tttttccatc aattcttgaa ttttgttcat gggttagttc
    19621 ctccaatttt tcattaaatt aagccgactc attaacgaat cggctgggat agtgttatta
    19681 atttcttctc gttcctttgg ttcaggtggt tcaatacgac acttcttagc aatcgctgaa
    19741 gtcagtgaat tagttacttt gacttccgag tacatcgaat tattaaccgg aatatccggg
    19801 tcaatctcac cgatgatttc atcggcaaat cctaggtgaa cagctttatt tgcgtccatc
    19861 cacgtctccg catccatcat atgtgccagt tgcacacgcg ataaattcgt tttacgttca
    19921 taggcgttga taatacttgc tttcacttca tcaagcatgg caatggcttt ctccatatca
    19981 actttttcgc caaacgcaac cgctgacgga ttgtgaatca tcaccatcgc cacaggactc
    20041 atgagtacct tgtcacctgc cattgcaata acacttgcgg ccgaagcggc gatgccgtca
    20101 atcttgactg tgactttacc tttgtagtca gcaagcatgt tgtaaatact tgcggcagcg
    20161 acacaatcgc caccacccga attaatccag actgtaacgt tacctccacc tgaatttaac
    20221 tcatctttga aaatctgcgg tgtgacttca tcatcaaacc agctctcctc agcgattggt
    20281 ccgttgaggt agagggttct ctctaggatt tcctcttggt tgtcttgatt cagaaccgtt
    20341 tgattcttcc acttccaaaa cttcttcatc ttgttcctcg ctttcttctt tgtttacgcc
    20401 ataagctgcc ccggcttgtt caattggcag catattgcca ttgaagtaat atttcgcacc
    20461 agcaccacca gggattgggt tcaagtcctc aagttcacgc acatcatcag gagataggaa
    20521 cccattttga ataccggttg catagccttg ttgacgtgtg gcatagtcac cacgcaataa
    20581 gccatccaca ttaaacttta tgaaatactt ttctttttca tcgcgactta acagtgattt
    20641 attaagtgct tgttcccaac gattgagcca tggtgataat gagaacttca caaaatccaa
    20701 actcaaatgt tcaacgtttg agaaggtcgc gtggtctaag tcgccaacca tgtgaagggg
    20761 cactcggaat atccgtgcga tttcttccag ttgaaactta cgtgtttcaa ggaactgggc
    20821 ttgttctggt gacatggaaa taggcgtata cttcatgcct tcttcaagta ctgcaacctt
    20881 ggccgcattt gaactgccac caaatgtcgc attccaactt tcacgaagct ttcctgggtc
    20941 tttcaaagtt ccaggatgtt ctaggacacc accaggattg gcaccattgg caaaaaattt
    21001 agaaccatat tcctctgttg caatcgcaag cccgatagca ttcttagcca tggcaattgg
    21061 tgaataacca accaagccat caaagccaag gccaggaatg tgaagcacgt cagcttttga
    21121 aagtctgact tgcccgacat cagcttgata taaataatag atttccttgt tgtcatcgcg
    21181 attaaccgtc atcttatctg gcattaaagg atacaaagct acaacttcac ctttgccatt
    21241 tcggataatt tgcgcatagg cgttacccca aagtagcagg tgagtcatta aggtttcacg
    21301 aaatacaaat gatgtcattt ccgaatttgg ttcatcatgc agcaagaaat acaacgaatg
    21361 tttcattgct ttatctttgc cgccagcatc tcggtattgg taaaagtgta atggcaatcc
    21421 tgccacggct tctgatagca cgcgcacaca tgagtacaca gccgtcatct gcatagctgt
    21481 ttgttcggtc acgcttttac cagctgatga gccgccaaac ataaatcggt atggattcat
    21541 gatggtttga tttgttggtt tatcgcgtga tttgaatagt gattttatta tgcccattga
    21601 ttgctccttt caaaatttga gtatcaaaaa agcacctacg atttgtagat gctgtgttca
    21661 cattagatat aaaaccgact tcctcttgac aaaattatct caattgcatt atttaaaaag
    21721 gtatttactt cctctaaaca tttcgatttt tcgtcaggtg ttaaggattt tctttgataa
    21781 ggaaagaatg aactacttgg tgcaagtttt ttaaacgtca tataatcttc atcatcgaag
    21841 aaatcttgta aataatttag ttctataaaa cttttccaat ctttgaattt agagaaatag
    21901 tctttattat taacgataaa tttatcagcg ttagttgatg aaccatatcg tttatctctt
    21961 acatcagaat aaaataaatc aggaaagtca tgatacggcc ctttaccttt aacaccattg
    22021 agagaaggtt ttgatacata tagtggcata aaatttgcaa gagtatgact taaatcatag
    22081 aattttacaa atttcacttt catctcttcg gtgatatcat cggaatataa aatgactttc
    22141 aaacgcccat aatgactatc tggcattgaa aagttctttt gattatagca tttcaataac
    22201 cgtatcgttg caccagcaat tgtgttgaaa gaaataatcg tttctcccct ataattccca
    22261 attccttcag catactttgt caaatcttca gttagaattt ttccccacca ttttctataa
    22321 aacagtctag aaattaatgt gttgtccggg tctgaagata ttttatctgg tgctacttga
    22381 atcaaatatt cattcataca catacacctc caaagacaat tatatcaatg gattgtaata
    22441 aaagctcata tttcacagaa acaacaatcc cctatcatca taaacactgg caccattatc
    22501 atttccacaa cgaattgcac gatccagagc cataattgtc gcgatggcac catcaatctt
    22561 ctcagtggat ttttctttat cagctttgat attacctgcc gggtcagttc ggacgaaaat
    22621 gttgtccata ttccaacgaa gaaccggatg accgccatgg gctaatttct gttcaagagt
    22681 caacttcatc aactctttag tagccgggct catatccttg aaaccttgac cgaaaggaac
    22741 tacagtaaat cccatacctt ctaagttttg aaccatttga actgctcccc aacggtcaaa
    22801 ggcaatctcc cggatattgt atttctcacc cagttcctca ataaattttt caatgaaacc
    22861 atagtggact acgtttccct cagtggtttg tatgtacccc tgcttggccc acaaatcata
    22921 attcacatgg tcgcgtttaa ctcgtaagtc caaggtatct tctggcaccc agtaatatgg
    22981 cagtatcaca aacttgtcat cctcatcttc aggtggaaac accaaaacga aggatgtaag
    23041 gtcggtcgtg ctggaaaggt cgagacctcc gtaacaaact cgacctctca aactttcttc
    23101 atctgcattg aatgaacaag cgtcccattt atccattggc atccaacgca ctgcttgttt
    23161 cacccattgg ttcaggcgaa gctgtctgaa tgagttttct tcagctggat tttgtttagc
    23221 cgaatcacaa gcagccacca ccttttcttc tgcaatcgtt atcccaagtg acggattggc
    23281 tttcttccaa acttctggat cagtccaatc atcatcttgc ttgataccgt aaattaacgg
    23341 atagaatgtt ttatcatgct ttcgaccttc aataatgtcg agagcttttt gatgctgctc
    23401 ataacaaatt gaattggtat cattccctgc cgttgtaatc aaaaagaata gcggttgcgt
    23461 tctagcatcc cctgcccctt tcgtcatgac atcaaacagc tttctgttag gttgtgcgtg
    23521 aagctcatca aatacaaccg cagaactgtt taatccgtgt ttagagtatg cttcagctga
    23581 taatacttga tagaaactat tcgttggctg ataaataatt cgtttttgtg aagtcaggat
    23641 tttcactcgt ttgcttaacg ctgggttctg gcgaatcata tcaacagcta cgtcaaatac
    23701 gattgaagcc tgcgctcgat cagcagcaca gccatatacc tcagcgcgtt cttccccatc
    23761 accgcaagtc aaaagcaaag ccacggcagc cgctaactca ctcttaccat tcttcttagg
    23821 tatttcaaca taagccgtat tgaattgtct gtagccattt gccttaatcg ttccgaataa
    23881 gtcacggata atcttttctt gccaaggcat cagtttgaac ggtttcccga accactgacc
    23941 tttcgtgtgt ttcaacaact caataaaggc cacggcataa tctgcacgtg acttatcata
    24001 atgggagtct ttgtccataa attcagttgg tttgtaatta tcgattggaa ctcactccct
    24061 tctacgcaaa aagcagctgt tgtcagctgc cttaatctct ttattccatc atatcggcaa
    24121 tgaactgaat ggcttcaggg ttgttcttgt tatcccgcag caattgcacc agttcttgcc
    24181 aatcgtagta tccttcttca atgccatctt cattatctgg catgtaaatt tgataatcca
    24241 tcatttatca ccctccaaga taaagcggct gtactcatct ttgtgttctt caatgaaaat
    24301 caccaactcg taaaagtgcc ggtcatgtgc aattcgctgc actgcaatcg catcaaacat
    24361 atttgtcaga ccactttctg caatctcaat gatttgttgc ttaatctttg cgttcatttt
    24421 gaccgtcctt tctgcctgcc tcataggccg caattaaggc atcttccaaa caccaaaccg
    24481 caatgtccaa gaagtcttca cggtcgctgt tgtgggcctt caggtcgcca cgttcaggca
    24541 actgaacgat gtaattcttt gcgatgttgt aaatcttacg tttactcact ttcatcttaa
    24601 agcccctttc tgatgtagat ggtatcttct gtttttgact caaggtcaac ccagaccaaa
    24661 tcgtggttca atatcatgtc cgtcacgtat tgccaagcat cattgaaggt ttcaaatcct
    24721 tctcttctac cgttttgaaa gaccaattcg aagtctggtt tgatgatgtg caccacatct
    24781 tcgccgtata ccgcgttcag tgttgaaccg ttgtcccatt ggataaggat tgaaccaatg
    24841 tcgtcaacac cggttactgt gccttgtgtg cccttcgctg gggcatgtgg gtcgttcatc
    24901 ttgatgagtt ctacacgact accttcaggg taacgttttt tcataccctc gatttgttca
    24961 tgagttggaa acataacttt gtcttccgta ttcttttggt agtctatata tcactctaaa
    25021 gggattttat agcaagtgat acgttattta ttcaggattt tctaggtcat taaatgcgat
    25081 agtctttcca tctcgaatca ctgaaacgtc tgcatcgctt ccagcctgct cgatataacg
    25141 cttaacaatc acatcacaga acttttcatc cagttcgatc gtatggcaaa tccggtcggt
    25201 ctgttcgcaa gcaatcaaag ttgatccgct gccaccaaaa ctatccaaca cgatgcagtt
    25261 gctgagtgac gaattggtaa tcggatatgc caacagcgga attggtttca tcgttgggtg
    25321 gtcaccattc ttctttggtt tatcaaattc ccagatggtc gattccttac gtccggtgta
    25381 ccattgatgt ttgcctttct tcttccagcc atagagcacc ggctcatgct gccattggta
    25441 aggcgaacga ccaagcacta gactctgttt cttccagatg cacgtacctg ataaatagaa
    25501 ccctgcatcc acgaatgctt ttctgaagtt caagccttcc gtatccgcat ggaaaacata
    25561 aatactggcg tcgttggcca tcacactttc catacaagaa aaggcatcat acaagaagtt
    25621 gtaaaatgcc tcgtccttca gattatcatt tttaatcgtt cccgctttac cttgatatga
    25681 gaccccataa ggtggatctg tcaccacaag atttgctagt ttgccgtcca tgagtttctc
    25741 ataagtttca ggttttgttg agtcaccaca aactaaacgg tgacgaccaa gcaaccacaa
    25801 gtcccctgtt ttactgaagg ctggcttttc aagttcctca tctacatcaa agtcatcatc
    25861 ttgggcatca tcattaccgt tgagtaattt atcaagttca tcagtttcaa aaccaagcaa
    25921 atccaaatca aactcttcac cttgcaggtc tgataactca accgccaaca tttcatcatc
    25981 ccaaccggcg ttcattgcta gtcggttatc agcaagtatg tacgcacgct tttgtgcctc
    26041 ggttaggtgt tccacaaaca cgcacggtac ctgcttgata ccttcttctt tagccgcaag
    26101 aatacgtccg tgacctgcaa tcaagttata atccttatca acaatcaccg gatttatgaa
    26161 cccaaactca cgcatagaag cttgaatctg tttcacttga tccttactgt gggtacgggc
    26221 atttctggca taagggacca gcttatcaat atcaactttc tcaaaacgtt ctgttgtgtt
    26281 cattagaaca acccccattc tgctagtttc tcaaacccgc caagctcatt cacataatca
    26341 cgcgcaactt ccacgatttc agaataaggc acgccatcaa tctcagtgtc accaatcgca
    26401 cagcttaact caacgggcat accagtttct tgagctttca agaatgcgta gatgttcact
    26461 gacacatcag cttttgataa gtctttccca tggataccgc cacctgttac tccgtctgcc
    26521 atatcggaac caagcttgcg attagtcgca cctgtatcca catccgggcc gccagtccaa
    26581 tcacctagag ggttgactgt agcgtttgga tatttttgtt ttaactcatt tgtctttgcg
    26641 tggctctgac aaattatcaa gcgttcacca tcaagaatgt actttccatc aaatggatgg
    26701 tcctgatata agtcacgcgc aatttttgag agctgtaact gttcatcagt caatggcatc
    26761 ccacgaaaga ttccattatc gccacaacga atttcagtcg cttgattgtc agccaactct
    26821 ttatcttggg gcacaatcac cacatctttt ttaattttgc ctgcgatacg attaatcgct
    26881 tggctgattt cattcaaatt taaagcagtt gaagtttcaa taatcacatg acagatgcca
    26941 tgaccaatta aaacctcaac tgctatcttt ggattatttt cttctgcgta tgccaagtcc
    27001 acaatggctc cggcaatacg atctgcaact ttatctggat gttgcggatt aaccttctct
    27061 atcataaatt ctgttccttt caaccattct tagaacgtaa caatcgttcc atcgcatccg
    27121 tattcggatc tcctgtgtat tccactgatg cattctcttt tacaatctgt tgaatctcat
    27181 accacgtcac atttgcttgc ttttggaagt tctgcgacat cgccacaaat ggggaggcca
    27241 tgactgcctt ggtagtcggg tgttttccga gcaaaccata ttgactcaga gcattttcac
    27301 attgaacaaa acgcgcaaag ttctgagcgt aggactcaac cattcgtggg gctatcaacg
    27361 tttcgcatcc gcggtctttc aaccattgga acgttgagtt ataaatttca tcagcgccta
    27421 gcacagtgcc atccttctgt tttgcagaaa ggtaagcact tggttcagga taatcattgc
    27481 cctcaagctc tacaccctca ccaatatctt caccaattaa taattcaaca tccggtaagt
    27541 caaattccat ccgcgatgct ttctttccag cagcaacttt atcaacgagt ggctgtgagc
    27601 gttcccctga acgaggtcgt ctgccaccgc gttgtgttcc atcttttgcc aaaatttatc
    27661 acctcttttt cttgtttggg gctatccccc gtttgaactg aacttttttc gcgtaagggc
    27721 ctagcgcgct gtacagttta gaagggttta gggataatta ccgcccctcc cccccttgtt
    27781 atttttcaca tgattgtttt atcttttgtg tttcgtcgag tcatgtgaat tttcgagtga
    27841 catgatttgc agacactcat caaattattt tcatcatgcg taccaccatc acttaattcc
    27901 ttgatgtgat gcacaatctc aaccgaagta aagcgtcctt ccttcagaca catctcacat
    27961 aggggatgtg ctgccacgta ccttgcccgt attttcttcc acgctctgcc gtaacgtttg
    28021 ttgattgccg gatcacgctc gtatttctcg taacgtttcc tcgcaagtgt tttatgttca
    28081 tcacaataag tatcatgcgt acggttcgga caacctggga acttacaggg gttagctggt
    28141 ttcattggca agtggggtca catcctttcg ggcaaaagaa aagcccttga aggatttttc
    28201 ccacaaaggc ttcgagtatt tgttcgatac ttctattgct ggtattgctt gagtatcgtt
    28261 tgtctttttt gacatcttaa tgatatcact aaaggctact gacattcact gacacatttc
    28321 aaatgactat gggttcatct ggtagcttca agttctcaag agctttgcta tgccaacgaa
    28381 agactgtccg cacgtcagcg tgaagatctt cagcaatctt gtcccagctc tcttccaaca
    28441 ggtatcgatt gattaacaca atcttttcgt ccacgtttgt gagttcatca atcacgccac
    28501 gcatttgtcg tttgaggtct acaaacttat caatctctgc atcaatttca gcttccaatt
    28561 ggtaacactt ctcaaggtta cgaacaaatg gtgcctcagt attccgtgtg ccacttgaaa
    28621 tcttttcttc gaatgttgtg gaagaaactg tcaccgccag ttgatagagc tccgccttct
    28681 ctttaatccg gtcgtcaatt cgtttattca aacggtacgc ttgtttcaag tattcttttg
    28741 gtgtcacttg cttccctcca atctagcttt cactgcatca atcaaacatg attgtgtttt
    28801 atcttttcgt ttcaaagcca acatcacatc ttcatcaatc gtatctttcg caataatgtg
    28861 atgaatgacc acagtatcac tttgcccttg tcgccacagc cttgcattgg tttgttggta
    28921 aagttccaaa ctccacgtaa gaccaaacca tacaagtgtt gaaccaccag cttgtaaatt
    28981 caatccatgt cctgcactag ccggatgaat gacagcgatg gggatgctac cttcgttcca
    29041 atcaataatg tcctgagacg ttttgatctg tcgaacgttg aatcgttcct tgatacgctc
    29101 cagatcatgc tggaaccaat aggcaatcag aatcggtttg ccatttgtac cttcaatcaa
    29161 atcttccaaa gcatctaact tctggtcatg gatgtgatga ctcacattaa actcatcata
    29221 gattgctcca ttcgccattt gtagcagttt attcgaaagc accgctgtat tggctgcatc
    29281 aatggtggct tctttcaatt gtagcaccaa gtctgcctta atttcatcgt acttttgctt
    29341 ttctttgagt cccatatcaa ctggcacttc attcatcaca agctctggca tatccaagaa
    29401 atcgactgac ttcattgaaa tcgtgatatc tgaaatcttg tcgtagattt cttgttctgc
    29461 atcttccctt ggtttccaag aataaatctg catgccattt tgcttgtcag gtagaaagta
    29521 tttatctcga taacgtgaaa tataatagcc caatctctcg cccatatcca gaaccttgaa
    29581 ctccgcaaac aaatccatca aaccattact tgaaggtgtt ccggtcaatc caacaattcg
    29641 gtcaaccttg tgtctgactt tcataaagct agtgaaccgt ttagctttta agtttttgaa
    29701 ggacgacagt tcgtcaatca ccaccatatc aaaatcaaac gtaaaacccg atttgttaat
    29761 cagccagtcc aagttctcac gattgatcag atagatgttg gcattatttt gtaatgctgc
    29821 cattcgctga cttggtgttc caagaaccac ggaatacgat aaatctttca aatgatccca
    29881 cttttgaatt tcagttggcc atgttgattg cgtcacgcgc aacggtgcaa taattaagac
    29941 acgttgtatt gtaaagtcat catgcatcaa gtctttaata gctgtcagcg ttgtcaccgt
    30001 tttacccaag cccatgtcca atagaagggc ggcagtttta tgatctttga taaaatcaat
    30061 cgcatatttc tggtagttat gtggtacgaa cttcatcaga catcacctcc tattctttct
    30121 agtaactcag gtatctgatc aatctcatca agcacgaaac actgaaaccc taaatctgtc
    30181 agttgtttca tacgaaaaag ctgtaaagac cgtggcttct tccctggcgc cttcacttcg
    30241 acaaatccaa gtttcccatt tggcataagt accaatcggt caggcacacc agcaagcccc
    30301 ggtgatgtaa acttcgggca aataccacct acccttttga cggctttcac tagtgcttgc
    30361 tcaacctgct tttctctcat agccacaccg tcaaatactc gaaatacgca tcacgcaaga
    30421 tttccaagta ttccttttct gcgcagtgaa tcatcagatg tgcgacttgc ttatcgaatg
    30481 atttcttcca aggatacgtg acatccgaca tggcaaactc gtaaaactca tcaaatcgta
    30541 aatcacgttt accgattcca atttgtaacc actgtttaaa tttcatttag atattctcca
    30601 atctgcaagt atggtgtaac ccgtgaatac gcaatacata actttatata ggtggtaatt
    30661 ttttatccct taagaaaagt tctatataag acctacacag gttacacttt cctttaaaat
    30721 caactattcc aagaactcgg atttgagttg caaaccaaca atcttgtttc ctttattagt
    30781 tcgccggcgc ttaaatccgc ccgcctccag agccgtgtaa aaatcggctg aactacgaat
    30841 gtactcacca gttcgcgtac aaaacgcacg atactctgca tacaactcac cagatttttc
    30901 ttcaaagtca acagcagtct cgcagcactc gtctaagaaa tggccaagcc aatcattcgc
    30961 cgtcttatat tcagaaattg cattagcgac aacttgtgga accgttaaat gaaagtctgc
    31021 ggcgatgact tttttcgcac cgtcaaggat ccattgcaaa atggctccgc cggcattttc
    31081 aaaaaggtag tccgcataat tcttgatgtc cttattgccg tcaatcgtag caaggaatgg
    31141 aatcacaatt aaacgacgcc aggttccttt atcaatcgct ccaacttttg ggaggtggtt
    31201 ggtatagagc accaaagtat gcgttgggac atacttgaat gggtctttgt atttcttctc
    31261 agccgcaatt tcatccgtcg agcagagctg cttaatgtta gatgtgttga gtcgcatccc
    31321 ctcttcaagc tcggctgcga tgagtaggcg tttccccttc gcttccgcaa gttcaggttt
    31381 cacattgcgt cggatttgac ttgtaagaat atcagctgag atgctgccag agtaattacc
    31441 gagcacacgg ctgatgacat tccagaacgt ggacttcccg ttacgccctt caccatacga
    31501 gatgataagc gcttcaacgt aaactttacc aatagctgca agtcctacaa tcatctggac
    31561 ataatcaatc aactcttggt cacctacgaa aatggtgttc agagcgtcca accaaatctg
    31621 ctgattggcg tcactcgggg cacattccgt ttgcttcgtg atgtaatctt ctgagttgtg
    31681 gtcttgtgat ttgcctgttc gcaaatcaaa ggttgctgtt ggtgtgttca gcaagaactc
    31741 gttcacatct agtaaccgtt gctcgatttc aagcatgggc tttgcctcgg ttaatgctga
    31801 cgagagatac ttgctgtcgc ggcgtttgac agcaaacttc ttatactctt gtgcgtgttg
    31861 gtacaaatca aaactatgac gttgcgctgt gttgaacatg gtgactgcct ttttcggacc
    31921 aacactagca agtacatcga acgcgccatt actaaccatt tccttggtac gcttctcaat
    31981 ttcggtttca gattcttcca gttgacgttc tgtcaatgcg tggatcacac cttgcgcttt
    32041 tggtttcgac tcttcccaat agctgccgtt gtagacaagg taaccagttg acggtgagta
    32101 atgaagctta cattcatact cgcgagctaa gaccgttgct tgacctaagt ccgagtagtc
    32161 ggttggctca agtgataaat cagagttgta ctgctctgga ggaatgtagc cttcttgttg
    32221 tttcactttc ttcccaaagt tgaccgcgct attccagata gctttaagct cctcctccgg
    32281 cagtggcgga ttacattttt gggattgttc gagatacttg tgatacgtat cttccgttgc
    32341 accgtagcgt ttgatgagct ttcctgcgat gtgactcatg cttgaattac ggctgccttc
    32401 gctaatctct gactgcgcat tatcccaatc agcaaacaga tcatcttcga gaaaatcagt
    32461 gatcaactta ctaccttcat agatttcaac ttcagagttt ggtgttccga aaagcaaacg
    32521 tgccgcatcc atcgcattgt tgtcaaagta tgggaactca ctttgaatct gcttcttgag
    32581 ttccacatat tcatctttat cagttgtggc aggaatcggg aaatatacgt gaaaacgtgg
    32641 acgtgctgat ttgccacctt tgtctttcat gttgttacgt gaatagctga ccgcaaacgt
    32701 cacatcagga aactgcatcg ccacttcgaa aggggctacc caatcatctg gattgtctga
    32761 gtggtcattg tcgcaatcaa gtggaatcac atcagactgg atgaagttgt cacctttgcg
    32821 atagttgcct ttgtactcag ccatcacatg atccagcttg accgtggcaa caagtgtttc
    32881 tttatctgtc accacttgct tggttgggaa caatccattc ttggaattac ccacctgatt
    32941 ggatgtgaat aacgtcatct taagcatttg agacctcctt catctcatca tcgaagtacc
    33001 tgatatgttt cttccaatac tccgcttgtt caatttcaac tgccatgcct tgggagatgg
    33061 tgtcacccac cacccagact tcctcacatt tgcccatgag aacaagtgac atgtgcattg
    33121 ccacatcacg ctccgttgcg tcgtccatga attgtggata catcagatgt gccgcaactg
    33181 gcatgtagcc acggtcaaga gcaatacggc agtaacgctt agtgttttta tggttgacga
    33241 caacatttcc tgagaacggc gagcagacat agactgatgt tctgtaatct ttacgtctag
    33301 gcattattat cacctcgctc aatcactggt aagatgttct tcttcgattt cagcaagtcg
    33361 tacaagaaca aacgaccttt ctgcgtccag taggtatgca tcacgctgcg ctctgcatct
    33421 atcgcatgag tttttgattg tgtgtagcct tgattggcat acttctgata aagcaaccac
    33481 atcttgcctt gcttgtactg aattcccagt tcatgaagca acttgttcat tgcctgtggt
    33541 gacataccgt agtccttcga gattttagaa atcgacatca cagaattgtt ttgaagaatc
    33601 aaatcgtagt agctaacttt tggtttcatc tccaaaatca tttgttgttg catcgccacc
    33661 atttcttttt tctcttgtag ctgcttcaac actgaaatgg cccatgctgg atcttcgatt
    33721 gactttgtga tgaaatcttc tgtcgcgtac acaccgtgtt ggcggataga cggcaagacc
    33781 tcatttgtca cccaacgctt gaatttcttc gcgttcggca tcttgctaga aatgatgagt
    33841 gagtacaatc cactttcgtt gataaggata gccattgtcc cattaacgct gaacgattcg
    33901 ttcaccgtct tatcttcctc atcaacatga tctcgtaatg ctttttgtgg atttgtgtag
    33961 ccaagaattg atgctacatc tttaccaaca aagtgaggga catcgccaat catcaccgtg
    34021 cgtacagagc caaactccgc attttcaaaa actttcaatt cgttcatctt gtgaacctcc
    34081 tgtaatagaa ttttcaggag aggcattttg ccccttctaa cttacaggcg aagaaaatgg
    34141 tcgaaagtaa actctgactc gaatttattt ttgatttttt tgccttcgtc tataagcgaa
    34201 gaaagaaccc aattcgaacc ttatttttga taaaaaaatt tgaaaaaaat atggagccat
    34261 ttctgactcc acattgacta atccttttga tagaattcac actcgtatcc atcagcgttc
    34321 agtagcaagc cttcagccca cttaggcgtt tgactcatca actgacagat gtcttcaacc
    34381 ttcgattcca ttggcacttc gataatcact tcgtcgtgga cgtgcccgac aatgcgatac
    34441 ttacgaagat tctccattgc gaaacagaga atgtctctag ctattgcctg cacgatgttt
    34501 tcgacaaact tgggaccata gctttcaaga cgttcccatt tcttggtagc accgacacct
    34561 tcataggtaa cagcttcacc gccgaatttg ttctcaccaa tctttggttt tacataaact
    34621 aatttacgtc ctgacaagag ttggatgaac agaaaacccg acttgtattc aaacttgatg
    34681 ccatgcgtcg ctgtgctccg tcgttctttc acacaagtct ttactgcctt atcaacagcc
    34741 caccaaagct ccgttatgtt aatgtttgag tctcgccaag aattaacaag cggctgtaat
    34801 tcatcctcag ttagtcccat ttcaagagca tccattgctt tcaatgcacc caccgatcct
    34861 ccgtacccta gagcaagctc agcaattttc cctttttgac gtagatgccc attcacgcca
    34921 tgcttttcaa ctggcacacc gaacatctga ctggcagacg cacagtaaat atcgccacca
    34981 tttttgaata cttcttgacg ccacttctca cccgacagcc aagcaatcac acgtgcttca
    35041 atcgctgaga agtcagatac gataaatttc atcccttcac gtggtacaaa cgctgtccgg
    35101 aggagttctg agagtacgtt cgggacattg ccgtagagaa tgttaagcat ttcaaaattg
    35161 ctttgtttta ctaaactacg cgcttcagat aaatctgaca tatggttctg aggcaagttt
    35221 tgaagctgga tgccacgtcc tgcaaagcgt ccggtacggt tggctccata gaattggaat
    35281 aaaccacgag aacgattatc agaacacgca aagttttgca tggcttgata ctttttcacg
    35341 gatgacttgg cgagttgctg acgtaagacc aggacttgct ttaaattttc atcaataggt
    35401 tgttcgagta gttcggccac tgccttttta ccgagtgact cggtttcaag tccgtggtct
    35461 agtaaccatc cccgcatttg aagcacgctg tttggatttt ctagtccagt taattcttgc
    35521 aattgttgat tcaatccagc ttttgataat tcatccaact gaacggcagc ttccacaaag
    35581 tccttatcaa tctcaattcc tcggtcgttg atttcctgat ccagccagta ttctttccaa
    35641 acagaatcaa gcactggaaa attggaaagt tttccttgga tagctatttc aacttccaca
    35701 tcacgactat tgtatgtctt aaatgctgac catttagttg gcgcatggtg tggtaagttc
    35761 ctcgtgcgtc caccattaac tttggtcgga ttgcatggca cgcaaaagta tcggatgagg
    35821 tcttttcctt ctgtgagctt ttgtttatcc aaaccaagaa cagcaccaac tcctacaagt
    35881 gacaacggca aacctaatgt cgcaccccac accattgtgc attgccaccc tcgtggattc
    35941 aaaaagccat ccaaccccag aaagcgtgac aaacacacac gttcaaaact cgcattgaag
    36001 gcgtgtttaa tcactgtttc atctgatagc gaagttaata tttcagccgg gatagtttcg
    36061 tccatcttca gatcaaccac ttcgacggga ccaccatcta cactgtatgc aaatagcagt
    36121 atttcaaaat cgggggactc tgaatatttg tagacaccac atttcgataa atcgacacta
    36181 ctatacgttt ccaaatccaa tgacaaatgt ttcattttct tcctccaatc aaacaggcga
    36241 cagcacttct gccatggccg tttttgtttt aatgattaag ccaagaaatc atcatccgcc
    36301 aaggtttcaa actcatcgct tgcagatact tgtgagccga gtggttcgcc gtcacgtaca
    36361 aactggatat tcccaagccc tgccgctacc ccgcgatttc catttgcgtt aaaggcatag
    36421 aaattaatcg acacacgagc gtagcaacca ctatacactt gtgaacgatc caagattggt
    36481 tgaacgtgct gatccacgat ttgtggtgca gtggttgagt tcgcattaat gaagtaatgg
    36541 ccttggtatg cttcatcgtc gcgttcgata tccccatcac gtaatggcag cttgatttct
    36601 gctttgtttg gtttcttccc accgaatttc gcaagtccct cttcgattgc tgcatcgatt
    36661 gctttttcaa tggtatcaat cgttctctta tctgattttg gaatcaagac tgctgttgaa
    36721 tatttctctg cgcccccgtt gatggatgct ggttcccaac cgtggaagta tgagagacgt
    36781 gtgtttgtac cagtgattac tttgcttgtt ttagacatat tattgttcct ccgttaatgg
    36841 tttaaattct tcgtttaagt tgtttactgt gaccgcttgt cgtttatctg aaccatcgac
    36901 tagcgtcagc ttgccttttg gtttgatgat gtattcgccc aagatttcat tgaatttctc
    36961 tttaccgagt gccttcgtca ttgctgtgac accaagtaac tttttatcat atgggtcgta
    37021 tccagccttg atgaccgccg cagcgacatc attgtcatta gaatacttac gagtagagcg
    37081 accctcgact aatttgtgat gagtccattg tttgccaccg agtgcaagtt tcaaagcaca
    37141 gtccttgata tcgttactcc aagaaaccag gtcatccact ttaccgatga tttcttcgat
    37201 ttcatcatca ctaagaagct ctggtggttt gaactcatat tgcgccagtt tcaagttatg
    37261 gtctgctcgt gcacgacagc tattcttcgc cggacagaat ccacaccatt tcccgcattg
    37321 gtactcaccc tcacctttga aagctaaatc tgcggcaggc gacaacacat cttttgccca
    37381 ctgaagtaat tcttctttca gcagtgtgta cgtactcaca ttctcacgcc ttggctggaa
    37441 aatggtcatc ttgacttctt gaatgtcata aataacatcg aaaagctcca acgctcccaa
    37501 ggcataacac atcatctgtg ggttctgata cgcatctacc aaaattccta gaccgtattt
    37561 catgtcaata acagataggg ttccatccgc aatgacgaga cagtcgccgg tcccaaagcc
    37621 gtctgggacg taccttgaga agtcgagccg ctgttctact attacaatag agtcaactgt
    37681 tgtctgtttc gccttagcga cttcttccat gacgaaggtc gcataacttt cagcacactg
    37741 ctccatctcc tcgtcgtaga attcaagcgt agatgttggg tctgtgacgt cattaccaat
    37801 gaacttattc agtttgtact cacataaggt atgagcatca gtgccttgtt gggcatacgg
    37861 gctcgacttg ttttcaaagt gctctgacaa gcgaacactt ggcggacagt gaatccaccg
    37921 attacttgaa gatgcggata ggactgcgtg ttgtgttggc atctacaatc cctccgcatc
    37981 tgcaaccaaa gccgcgtagt tttctgtcgg cacatcactc aagcgactgg caccatattt
    38041 ctcaatcagt gctttcactt cagttgactt accttcttga gatttacgtg ccaacaaacc
    38101 acggacttct tcaagcgtcg gttgtttgac ttctgatttt gtttccattg tcggttctgg
    38161 tgtttcaact tgtggttcag gttcattggt tttcatcgcc acagccaact cttgtaagct
    38221 atcagctaac gcttggacgt cctcaacgac ctgaagaaat agttttgttt tactcacgtc
    38281 attacgctcc cttcttgtta acttcacgga tttcgactga ttcgattgtg tcccctggtg
    38341 tgagcacgag aattttgtcc atgttgccct taccgaacag ccaccgtgca aaccagcgag
    38401 gcaaatgacg cacaccacct tgtaggacag tttctttgtt acccgtgtca tcagtcacgt
    38461 ttactcggat tttgtgtttt aggttcatga tggtcctcct tcatctttat ttgtttagga
    38521 tctactccct tacacaatac aggcgaagga aaacggggaa agtaaactcc cccgtaactt
    38581 ttttctaaaa taattctttg atcttgtctt tgatgattcc aaaggtgcga ttgattgact
    38641 gttttttctt gtcaagcttg tccgccactt cagtctgaga catgttttca gtgacgagca
    38701 tgtgatagat ttcttggtgg tacggtaata attgcgtcac gacttcacgc aaacgatcga
    38761 tacgaggatc ttcttcatta tcttggtaag catcgtaaac caatgattgg atggggttgt
    38821 ccccctcatc gcattcatca tcaaattcat tgagcgacaa attgtagtta cgtttgaagt
    38881 aatcgttggc ttcaccatcc acgtattcgt ccattggata atagccgtgc tcattattaa
    38941 agtcttcacg gaacttctct ttccaagcct tgatcttttc tttttcttct tctgtgcgtt
    39001 caggacgtgc gtttttataa aagttgtaaa tcgcacggtc gtcagcgcga tgcagctcct
    39061 gaatgttaag ctcagtgacg ccgtcttcgc ctgggcgaat ctcaaccact tctttttgga
    39121 tacgtccatt ggcatcagta cgctcaaaca cgtacttgta agtgctgcgg tcgttttggt
    39181 gtgttttcat aaatctactc attagatccc tctttcttgc ctgaaagagc ttttgagcaa
    39241 ggaaaacaaa cagggaccgt gccaatacgt acaccgtccc tcatcacgaa aaaagagcgc
    39301 aataaagtaa ggcatacaat atctgaaaaa ttttctggat aaccagttct tttcagtatt
    39361 tgtaaccttg ccctattgca actcaggcat tgttatattt tattagtagg actattcgct
    39421 tttaagataa attcgttcac tttcttgaaa cataatgtag tgtttatgaa ccataaatga
    39481 taaaattaaa tagttaaact actatgtttt aattatagaa aaaggcctcc cttgttttcg
    39541 tcagtcatcg gcggcaaacg gcggtcaacg gcggacgtaa caaaggaggt tgtattttgg
    39601 aaataagtga aattactcaa acgctttatc cgtttatggg aagcgagtac gtgaagccac
    39661 aggatttttt aattgcttta ctggatagtg tttcaaatga tgacgaggcc accattttcg
    39721 gtttttctga tgataaccta cgaagatttt acaatggtac tcgaccatta acacaagagt
    39781 atgcagttcg ggttgctgga aaattcaaca tcgacaaatg cacgactttt atttatgaac
    39841 gtcttataga tgatgctcca accgtcctta agcaacaatt gattgataaa ggtgtggtaa
    39901 tccccaaaga tacagacgtt tctgaaatag tggcccatct tttacttgat gaaatcacct
    39961 caattgcttc tcaaacaaaa gtaaaaacaa aaaaggccaa gccgaagctt agcgaattag
    40021 agctcgcaag tttaagaatc aacactaatg gtgatttctc ttataatgat gaagtgaaaa
    40081 aattctttga cgatttgcca gttccccttg atattagagc tgaggagatg acgtatgtca
    40141 tagctctttt ttcagcatat gcagatgccg atggatacga agtatacgag catacaaacg
    40201 acctgccaaa ttcgcggcta aaggatttca agagacagcg aagaaattac tataaagctg
    40261 aaagcatacg tcgtggggtc cgtgataact ttacacaaga ggaaggaact gagcactttg
    40321 aagctttaaa agaagatatg tacgacggca tcgaggaagt ctatgaagaa gactacgatg
    40381 atggcgttga gagactcaaa gccgttctac agcagtcgtc tgttataaca ttaaacggat
    40441 caatcttatc gtttataccg ggattgctgc aaaattctgc taaaaaaggc atttgccaca
    40501 tgctggttaa tgacggaaag atcaaatggg tgactgaaga tgaatagttt gtttaatact
    40561 ccatttgaaa tttcggttcg agtaatgtta ctgctctctg aatttaaaga gcctgtcagc
    40621 gctgatagaa ttttaatcac agacttcatc accacgtatg gtcgcgactt tgaaatttcc
    40681 gattacaact taaatggtga taacagctat agattcagtg aatttattgc gcgacgtgaa
    40741 ttagttaaag atgcagtcaa aaatcttgta ctgcaacatg ttatccaacc atcgcaaagc
    40801 aaagaaggtt ttaagtatgc tttatcagaa gatggcctaa agttgatatc acaattcgta
    40861 tcggtatatg cagaaaaata cttacaaata gctgacgaat ctgttaaata cattcaagat
    40921 aaaagtgacg ttgagcttct gcgacaaata aaccaacgcg caaaggaatt taaaggagag
    40981 caatgataat gcaaaattat tatattgaac gtgttaccat aaaaggcaaa gatcgtgaag
    41041 atgcaactgt caagttcacc aatggactaa acataatttc tggcccttca aataccggaa
    41101 aaactagcat tgttacggca atcgattttc tctatggcgc gaaaaaagac gctgctccct
    41161 tttcaaccga tgcaaccgga tacagtaaag ttgaactgat tattcaatcg actaatggca
    41221 aatattcatt aactagagaa tttgatagca actccatttt ggtatccaaa ataacgagtt
    41281 caggtgaata tgtagattat gaaaaatata atgcacgatc tgggaaaaat ccaattagcc
    41341 atttttggtt acaactttta ggcatcgatg atgattatca gattatcaag aatgaggatt
    41401 tcaaacgtca aaaattaact tggcggacat tagcgcctag cacacttatt ccacaagata
    41461 gagtcaatac cagcgcctct gttctttttc ctggggaaac aacccaacgc acagcttttt
    41521 tatcatcgtt gttacttctg ctatacggtt cagattttgc gaactcggaa gaaaacgaaa
    41581 caaaagaaca taaaaaaatt agaaggaact cggtgattaa ctacatcaat tcaagccttt
    41641 ctgaattata tgaaaactac gaagtggcta aatcagaatt acctgaggct ccctctgatg
    41701 attttcaaca aattgtcgat ggaatgttcg ctgaacttga aacaattgaa aagcaaatca
    41761 atgatgcgtt agccagcagc aaaatcttga taaaagagat ttatcaaaag gaacagtatc
    41821 ttcatgaaac tcaacttctt ttgaataggt acaaacagtt acattatcaa tatcaatcgg
    41881 atattgaacg actaacacta atcgtagatg gtgagcgatt attaaatgat cataatgaac
    41941 atgatacttc acatagctgt ccattctgta acggcgaact aagtaaacct gtccaaacaa
    42001 cctacatcga tactgctagg tcagaattac attcattgct ctctcaatta tcagatttga
    42061 cggaagaatc tcatgatacc gaagttgaac tgcaagagtt aatggaacag cttgatgagc
    42121 ttaaaacaag aaagaaatcg cttgatgcag caatcaacga tgaactaaaa ccgaaagctg
    42181 agacgttaaa ggatcgaatc aataaaatcg aaacttacag ggatattaaa caacagctaa
    42241 tctcgatgga aaaaatgcaa aataagctta aaagtgatct tgaaaaaatc gagaatgagc
    42301 cagaagacga acgtatcctt tataaaccta aagagaaatt tgatgcagaa ttctacaaag
    42361 aaatgtctga gttactggac tccatgttac gtaatatgca ttataaacct aatgaatttc
    42421 gtaacgcatt tttcgccaag gatattttcg acatcaaaat caatggaaat ctaaaatgga
    42481 ccgaaaacgg acatggatat acagcgttta tcaattcagt agtggtaatg gcatatcgac
    42541 atctcttgta taatcacgct aagtacacac cttctgtgtt cattatcgat tcccctcttc
    42601 ttggattaga tgaacgcaaa aaacaagatg gcgatgacaa tatgaaagaa ggacttttca
    42661 aatactttat ggatcatcaa aacgaaggac aacttatcat cgttgagaat acaacgaact
    42721 tgccagcact aaactatgat gataaacaag taaatcagat tgtctttact gaagataaaa
    42781 acaatggacg ctatggatat ctatacgata tttataacta gaacaggaat gattacatga
    42841 agtttagcta caatccgctt tggaaactcc taatcgacag aggactgaat aaagcacaac
    42901 tcagaaaaat gactgatatc agttcagcaa cactctcaaa actaaataaa ggcgaaaatg
    42961 taacaacaga tatcttactt cgcatctgtg aagcacttga ttgtgattta tcggatattg
    43021 ttgaaatcgt tcgcgattaa atatcaaaaa tgcactgtat tcgattcatg agtacagtgc
    43081 attttcatat ttatttttgt aatccagcga ttacttcatt gattttcgat tcaatatcat
    43141 tcaatggaac catataccat tcagtagctt ttttgaagtt tccatcctga aagacaactt
    43201 gtttgtcctg gaatacatga tggagagtgc gctcaagtag ctgagcgttg acattttgta
    43261 cttcagcaga aagcatgacc ttcactggtg catttaagta ggtcacgtcg ttttccgcat
    43321 tggcaattct actttcaaca tcatttcgag tgaaaccaat cttatacagg ttattccact
    43381 tgctaatatc cggattcgtg cttaatgact tcagcacgta gatataacct gtcgtaaact
    43441 cttctccaaa gctttcagcc agtgtttcct catctaccgg ctctgtcacc atccttccat
    43501 gacgttcagt cgagtataga gacgaagcta aagattgtaa caacagattc ttattctctg
    43561 tcccattctc ataaacaaca tgcattctgg catttgtttt cccattatca gcgataaagg
    43621 tatccttcga aatggaaacg atatacatca ttacgccgtt ttggatgtag aatctatttt
    43681 catgaatatc atactgctca aacgggataa tttttcgtct gccagatgcg atatctgcat
    43741 gcacttgatc gaacaagctc ttatactttt caaaatgact tgcatgtttt ctgcggccaa
    43801 ttttatctgc agcgttcaca gttctacgat agcgggaaac atccaacaag tctgaaaaat
    43861 catcaatcat atcatctgag cctaagatat catctaaatt caactcgtca tcgatttcaa
    43921 ctgaatcagc gagaaactct ccatccggtt cgaattcttt ctccaataaa ccaagagtgt
    43981 catacttctt aaccttctca gacctttcgg gttttcctcg aaatccgcta agacgagccc
    44041 acaatgcgcg ttcactagac cattcaggag ttttttctgg ctctcgtccg ttttcattca
    44101 caaagtctat aatctcctga aatttagcaa cttctgggtc ttgttttaca actttctttt
    44161 tttcaacttg cttggttaaa tcatcaaaag cgtcatcttc aaagatgcta tctaaactat
    44221 tcgccatctt ctttcgctgc ctctctttca cgttttatct cagcaagtcg tagcaaagca
    44281 aaggccaagt atttttcacc atcatcttta ctatttcgat caggatttcg accattttct
    44341 gcacggaact tcttagcagc ttggtaggcc gcaaccaatt gttcgtcagt aaaatcatac
    44401 ttatttgatg acaaataatc ctgaataaac cgaagagtcg gtgcatcaat atcacgacta
    44461 atcacttcat acgcacgttg gaacggattc acttgatcga ttaaatcaat attcaattca
    44521 tcaatgttaa tgaaacgatc ggccatctta ataatttcgc ggctaccatc ttctgaagtt
    44581 gttgtccgtc cagccgcaat atttatatcg gcaactgtat gttgacgaac ggtttctaac
    44641 tcttcgttcg tcaaatcagg ataacgatca atgatgacac gtggaatcaa cgtcttatta
    44701 atcatcttcg catcagcacc tgaaacaatc gcattttgaa tatcttggct ctgtaaaatg
    44761 ctagatttca aatcttccat atcattttcg atgattttct ttgtacgttc cgtcgtatca
    44821 gcaggtttca gtcctctgat tgaaagatca cctggtttgt ctggcttacc atcttttggt
    44881 ttaaacttgt aaactggcgc caacacttgt tccataagta gagatgcaga aattgccttc
    44941 ataatactgt taaccgcgaa taatacttcg tcatcctgtg catctggctg cgcaataaga
    45001 tttgtaaact gtgcatgagt cttattgggg ctgtcacgag ttacgcgacc aatgatttga
    45061 atcacttcag ttaatgagtt acgataaccg attgtcagtg catattccgc aaatggccaa
    45121 tcgaagcctt cttttgccat tccaagtgca atgatgatat caagcttatc aagatcttta
    45181 atgtcatccg taatacttgc tagatatgct gagacttttt cacgtccttc ttcattcaca
    45241 aggtcggcca ctaatagttt tttaccatct ggacgttcga tttcataaat accatcctcg
    45301 ttctgtgtga tattaccacc gtccgtcaat aaatcaagta tttcatcaac ttccgcgtat
    45361 ttatctttag tagactcacc agaattaaca ttaggaatat ggataattga ttttttgttg
    45421 acatcaatta ctttatccaa agcatctgta tattttccac gatagaaact ataccccatt
    45481 gcaaaggatt tcagatattt atatccttcc agttgctcat aataagtgta agtgacctta
    45541 tcaaatttat cttcgtcggc tggttccaga atctgaacag agtcgccacg gaaatatgaa
    45601 ccagtcattg ctaagatatg cgcattagag tttgacatga tgctacgtaa ggcattacct
    45661 aacacagatt tatcatcacg gctcacatga tggaactcat caatcgctaa tagcgtattg
    45721 tcaaataatg aatcgtccaa ctcttcaaaa gcaaatcgca acgtagcatg tgtggcaatc
    45781 aggatattat cacttgggtc agtcgagttc atgaatttaa caaagtgttg aacctttgat
    45841 ttttcaccgc ccccaatagt taagttgtca cttggcttta cttcccaatc ccaataaaaa
    45901 ccaaacttcg ataattcagt atctttaaat gatttaccaa ttgagcgttc aggaactgca
    45961 ataaccactt ttttaattcc ttgattatgg agtttatcca agccaacaaa catcaatgca
    46021 cgtgattttc ctgaagctgg tggtgatttt accaacaaat actgtgacgc acgcttttca
    46081 taaacacgtt cttgcatttc acgcataccg aggtcatttg ttgtttttga ttgattttta
    46141 tcaaaattaa tttcaaataa attactagtc attgattgtc atctcctgat agagttttag
    46201 taatgttcca agtcgatctt cgtcagattc aaacgggcgt tgctgataag ctctatcaac
    46261 aatcccatcc agttcctggt gtttcttacg taatgattct ggcatactgt ctttattata
    46321 caaatcggct aacgtgccgc cttcgtattc tcgtaaatca agaatttctg tcatcacgcg
    46381 agtcatttca tttttacgct gggcggataa atcctgaatc ggaaaagtat tatagactaa
    46441 cccggctgaa taacggtacc gagtttctaa tttcccaccg acagagcgca gccacaccat
    46501 atgcattcgg gattctaaca gaccaagcag ccacattgga gcgttataga ttgccatagc
    46561 cgaatctgaa attatagtat cgctcccaac tatacccata ggaacataat ctcttttttc
    46621 tgatgacaca cgaggaatta gaatactatt ctcactggtt tcatcataac gaacttcacc
    46681 aaatgaccat ggcttctcag ctagtgctcg agtttctttt ctcttgcttt tttccctata
    46741 actttttaca ttttttattg tttctttaat aagagaattt tgtttgtatc tagttgcatc
    46801 ttcctcggtc aaccataaag tatatcttaa gtctccattt attaatcctg ttgatcctaa
    46861 atatttctta aataaatgtt taatatctgg atatttttga atcaaattcc agtattctgc
    46921 cactgaaaaa actagattac caccatcatt tgccatactg ccaaatttca aagagggtaa
    46981 atttgaaata ctatttcttg attcgttgac aaatacattt tcaccattag ctaaatatgc
    47041 attaatattt gaaacactca ttactgaccc attctctaaa tataacttag gatgaatatt
    47101 cacattattt gtcataccaa taatgacaac agtcacaccg gcattattct ttgcattgtt
    47161 gttccactta aagctctgat atgcaaatga aattcttacc tcatctttta aaaggtacgg
    47221 ccaaaggata ctaacttgtt caccttgaca aattgaatta gtagtaacaa atgcgaacga
    47281 gccatgagat gttgcaatat attgtttagc tttccaaaac cacccaaaaa tataatcaag
    47341 caatttatat tttcgaatat cgccaacaag tttttccata tcttcacgtt gttcagcatt
    47401 ttgaagactt ttgcccagat acggtggatt tcccattata tatatttcat gattttcttt
    47461 gagtcgcaac acctcattcc aatcaattcg aagagaattg gcacacacta tattcccagc
    47521 atcttttaac ggtaacaact cagcaatggt acctggtatt gcttcttcaa gctctttatt
    47581 catttgatgt tcagcaatcc ataatgaaag tcttgctact tcatgcgcaa aatcatccaa
    47641 ttcaatccca ttaaaattac caagatgaat cactgatagt ggcatggaat cattattttg
    47701 taagtcgccc aacaattgca aaatcttaat ttcaagtcta cggatttcct tataagcaat
    47761 gatcagaaaa ttaccagaac cactggcggg atccaaaaac ttaatattag aaattcgcgt
    47821 caacaaagca cttagatcat tgatgattgt tctcttatta tcgtttcttg ttttttcagt
    47881 aatgtccttc agctcgtttt cttcactgcg aagctttaaa gtttcaaatg actctgtcaa
    47941 atcatctaag aataacggct tgatgacttt catgatattc ggcacgcttg tataatgcat
    48001 accggcaacg tgacggtctt ctgctgaagc aactgattga atcattgcgc caaggatatc
    48061 aggattgatt tcattccaat tcaataattc ccctgcttca ataagcagct ctcttgaaac
    48121 ttttgaaaag gtcaaatcca catgtggctc accaaacagt tttccattaa catatggaaa
    48181 ttcaagtagc cactcatcct tgccattgcg agctaactca ttaatatcca agattgcaaa
    48241 gagttctttg attacttcgt taaaattcga accatcttca gccgttcttg tttttagaac
    48301 atttgtaaag gaacccttat tcataattcc tgtatcttct gcaaacaaca agaacagcac
    48361 tcgaatcaag aacaagttaa atgcatgctc gttggcatca ggattatctt tagccaccac
    48421 atcatacaat tttgcaaaac gttcagcagc ttttcggtca gctggatttt cagcttgata
    48481 atcagccttc tcaatgccat tccacgctaa gaaaaagtca gcattagccg gtagctcttc
    48541 aattggaata ttcaaagttt catgagtttt cgtatcaatt gcagcaatat ctaaataatc
    48601 gtttaccaag atatatcgtg gtttagattt ttgctctttg attgattgag caatcgcatc
    48661 aatcgcaatg acaacgttgc cttcaatttc ttcataatga agtttgttct taatcacaaa
    48721 tggttcacca ttatcgaatt ttgattttgc acgtgtaatc gacgttttgg ggatatcgta
    48781 caaggacaaa aattgagtga tgaaatcatc gcccctattt gaaagtattt tttttacacc
    48841 atcttcaatt tcagtgatgt ttaaaacttt attttttgcc atttatttaa catcctccgc
    48901 atctataaca tctgaactaa ttatttcact aacatcaaca tttaaggcag cagcaatttt
    48961 caataatgac tccattgcaa ccaactcatt tttaccgagt tttgctatta cattgggact
    49021 gacgccagat atttcacgaa gttgagtttt gttcatattc aaatcaatca ataacttcca
    49081 caatttatta taagaaattg acatactaca cctctattct actggtatct tcaattatat
    49141 catctatgag ctatcattat tatacgaaac atgaaatatt ttcgcgaacg caaactattt
    49201 ttctaaaata aaagccttca gcatagattt actaaaggcg agataacaaa tattaattcc
    49261 actttatttt aaccggaatc cccaattgac gagctagttt atcaattttg attctgacat
    49321 tctttttgtt gaaatttgca taaacccata ctccatcagc taccctggct gaatttggtc
    49381 ttccgttata catggatggt ttacgtccta aaattatagc gaacgacggc gtctcatcga
    49441 caattaattc ttgcagttga ttgttgtagt ttccatccgc aaatagctga gaaacaaaat
    49501 caaccaataa acctctagca gatttcgaag atatcttttt cccatcatac tcaatttcat
    49561 attgtccaga tgaactaaat tcataaattt gggtttcact gtcttcggaa gtaccaaaca
    49621 aatcgatatt tgcaatttca tctataattt caaacactgt gtcatatagc tgatccgatt
    49681 ttgctttttg aagtttatca atatacgaag tattaccagc agttgaattc atcatttcat
    49741 agtcagattt ttcttggaaa tcttggataa gtcgtttttc aagataatct gtatctgctt
    49801 ttgacatttt cccatctgct cttgtaaaga ataaaactga ttcaacccaa gctttatatt
    49861 ccttgagaaa gtgttgagat aaacgaagtg aaattgattg ccctgctgcc tgacccacgt
    49921 aacgcttatt accaccaatc aaaacataaa cagctggata atttgaaaaa tcctgttcct
    49981 tcaaatacgg caaatcacta cgactcattg aaaccaagat tgctggctcg cgttcaaaat
    50041 aagaaatatt atctttctgt gtatctgaaa atataaaaat ttgcttcata agtttgtctc
    50101 ccttgaaaac tcgataatat attatatcac gtaaccgtta tcaaaatcat ctaacctatc
    50161 aagtctacgg tgtaactcct atcaactcaa agctccatag catttccatc aactctacgc
    50221 tcacccaaaa atcgtcattt caacacgaaa aaagagcaat ccggaaacca tcccgaatcg
    50281 ctctaatcct tgtcatatca acgttccaca cgtgtaagca gtcctacaca ctcgatgtga
    50341 tgggtttgcg ggaaaagatc tacaggctga actttcttca cggcataacc tgcttctgca
    50401 aataacttag catctcttgc aaaagttgcc ggattacatg agatatagac gattcgttct
    50461 ggtgacattt cgattgctga ttcaataaaa tgatgatcta atccttttcg tggagggtca
    50521 actacgatca cagatggttt gatccctttt tcttgccatt ttttcataac tttttctgct
    50581 tttccgactt cataatgaac attttcgatt ttattattta aagcattgaa ctgtgcatct
    50641 tcaattgctt ctggaataac ttccatccca tacaccttat ccactctgtc tgctaaagac
    50701 aagccaatcg tacctattcc tgaataagcg tcgatcacaa tgtctttttt atctaactcg
    50761 gcaaactcga tggctgtttg gtacagatac tctgcttgtt gagtatttac ttgataaaat
    50821 gactttgctg agattcgata ggtttttcct aatagctgat cttcgatata gtctctgcca
    50881 tataaaactt tttctttgtc tcctaaaatc acatttgttt tttcttcatt gatattttgg
    50941 atgaccgaaa ccacttctgg caattcttca tgaatagctt cagcaatttc cttggattta
    51001 aagaattttt ctttacgagt aactaaaacg accatcattt catgggaata atgaccacga
    51061 cggataatga tgtgacgaag ctgtccttca tttttttctt cattatatcc tcgtacttgg
    51121 aaccgttgca agatatctct gactactttg attgcttgat cgatgacagg atcttggata
    51181 taaaaatctt cgatagggac tagatcatgg ctattctttt tgtaaaaacc ggtggctaac
    51241 tgtccatcga tacggcgaac agggatctgt gctttattac ggtagccgaa tggttctgcc
    51301 attccgaccg ttggaagtac aggaacttct ggcatcttag caatcttcgc cattacatgc
    51361 tctacttgtt tttgtttgaa gacaagctgt tgttcatact tcaaatgggc taaaggtgcg
    51421 atacctatgc gaagtaatgc attgttttct acaggctgac gatcaggtga ttgtgtaata
    51481 aatgtttcga cttttccata tccaaactta ttgcctgctt ttactactag tatttctact
    51541 tcttctccag ggagtgcatt ttcgataaat aatggataac catcgatttt tgctaccccc
    51601 attccttcat aactcaagtc gacgatttcg actgtatatc gttcgttttt ctttactatt
    51661 tgctgttctg tcatattttg ctcctttaag cctatttttc aatcctactg aactctacta
    51721 taaatcagct aattacacaa ctaaaaccgg cactgacctt actacgttca gcaccggctt
    51781 ttgattaatt cttttgatta ggtgtttcgt ctgctatttt tccgtcaccg tcgatatctt
    51841 catccgttaa acgttcgact tcttttacga attctttgct gacaacctct aattcttctt
    51901 catctgtacc tgtgattgct tcatcaggaa tctgatcggt attggcaaag aactcaatgt
    51961 gttgatgaag attttggaaa tgcatcggtg cttccccgcc gtattcacca tctaagttga
    52021 tcattactgg tcgattgata tcatcttctg tttcaacgaa caaactgctt gttttggtat
    52081 agattattct tggatcatcg acatgtcgac cgccattcag catgagtgct gcaatatgca
    52141 agatctcaaa gatatttgct gtcttcacaa tgattaaaga aaacttccca tcatccaatt
    52201 tggcatctgg cgcgatctgt tcgaatcctc caacagagtt cgtcagccct aggaaaaaca
    52261 tcgaagcatt tccgatatat tccccttcat catacttcaa gcgcattttg attggcttta
    52321 ctcgagggag catctctgct cctttcgcaa gataagcaag ataaccaaaa atactcttta
    52381 gttctgatgg aacttcataa gtcaattctg tcaaatagcc acctgcagcg atattgatga
    52441 agtaactgtt atcagaaagg ccgatgtcca tttttacggt ttggtgtttt tttatcactt
    52501 ctgctgctgc tttaatatta tctctaggaa tttttaatgc tcgagcatag tcgttcgttg
    52561 taccagctgg gatgatggcc attttaggac ggtgttcaag cggagcgatt ccattaacca
    52621 cttcgttgat tgtgccatcg ccaccagctg ccactactag atcaaaacct agttctgcga
    52681 ctctacatgc ttcattcttg gctgaatctg gttctggtgt agttgcatag gcgctggctt
    52741 caaatcctgc ttcttctaat gcctgtagga tatccgccaa attttttttc aataattctt
    52801 ttcccgaggt ggggttataa attacgcgtg cttttttcat tttcagcctc tatcgtttcg
    52861 ctaattcatc tttaagtagc ttattcacga caccaggatt tgcttgtcct ttagttgctt
    52921 tcatgatttg accgactagg aagccaaccg cacgatcttt tccatttttg aaatcatcga
    52981 cagattgttg gttgttatcc aaaatttcat taatgattgg cagtagtttg ttcggatcag
    53041 ataattgcac ccaaccattt ttttcaacta cttcttgggc ttctccgcca tttgtgatca
    53101 attcacggaa gacttttttg gcgatttttg agctgatcgt tccatcggcg atcaaacgaa
    53161 tcattcctgc taagttctca ggtgtcaatt ttgtctcagc taattccagt ttctcactgt
    53221 tcaagtaagc agaaacttca cccatcaacc agttagatgc ttgcttggca tctgcaccat
    53281 tttccaaaac agcttcaaaa aaatcagaca tttcttttgt aagtgtcaat accattgcgt
    53341 catactctgg aagtccaaac tcacggatat agcgttcgcg acgagaagct ggcatctcag
    53401 gcaaactagc ttttactttt tgaatccatt cgtcatcgat cacgaatttc gggatatcag
    53461 gctctgggaa gtaacgataa tcactagacc cttctttgac acgcatcaaa actgttttat
    53521 tcgttgtttc gtcaaaacgg cgtgtttctt gttggatttc tcctcctgaa agaagaactt
    53581 ttgcttgacg tttttcttca aaagctaaac cttttttcac gaaagataaa gagttcaagt
    53641 ttttcaattc tgctttcgta ccgaactctt cttgtccata agggcgaaga gaaatattgg
    53701 catcacagcg catagagcct tcttccattt tcacatcgct gacttctgta aattggataa
    53761 ttgatcgcaa tgcttctaga taagcgtatg cttcttctgg tgaacgcata tcagcttcag
    53821 aaacgatttc gatcaaaggc gtgccttgtc ggttcaaatc aacgtaagaa taaccgccaa
    53881 taccatgcat gttttttcca gcatcttctt ctaaatggac acgttcgatc cggatttttt
    53941 tctttttacc ttctacttcg atttcgatcc atccgtcatg accgattggt tgatcaaatt
    54001 gggaaatttg atatgctttt ggattatctg ggtaaaagta atttttccgg tcaaaatgag
    54061 tatctttaga gatttcacag tttaatgcca aagctgcttt catcccgaat tccagcgcag
    54121 ctttattcat tactggcagc acgcctggat aaccccagtc gatcacgttt gtattgctgt
    54181 ttggttctgc accaaaatga gctggcgctg gagagaaaat ttttgagttg gtttttaact
    54241 cgacgtggac ttctagtcca atgactgttt caaaattcat tcgtctttcc ctcctaaaat
    54301 agctggttgt tgtttatgga attctgttgc ttgttcaaac gcatatgccg cttgatacat
    54361 cgttttttca tcaaaataat taccgatgat ctgcaatcca actggtaatc cttctgataa
    54421 acctgccggt acagacatac ctggaagtcc agccaagttg acagggatcg ttaagatgtc
    54481 actcatgtac attgtgattg gatcgttaat gttctcaccg attccgaatg caactgttgg
    54541 tgaagatggc ccgatgatca agtcataatc tgcaaagact ttttcaaaat ctcgtttgat
    54601 caatgtacgg acttgtcccg cttttttgaa gtaagcgtca taataaccag cacttaagga
    54661 gaatgtacct aacatgatac ggcgttttac ttcttcaccg aatccttctg aacgtgagtt
    54721 gacatataca tcatctaatg atttcacatt ttctgagcga taaccgtagc ggataccatc
    54781 gaatcgttgc aagttggaac ttgcttctga tgaggcaatg atataataaa cagccacacc
    54841 gtattttgaa tgaggcaagc tgacttcttc cacaacagca cctaatgaac ggaatgtttc
    54901 tgctgctttc aatacagctt cttttacgcc aggggccaca ccttcgccta agtattcttt
    54961 aggtaaagca atcttcattc ctttgatgtc gcctgtcagt ccctctgcaa aatcaggaac
    55021 tgaaacgcct gaagaagtac cgtctttttc gtcataaccg ctgatagctg tcaatgctaa
    55081 tgcattatct ttcaccgtac gggtcattgg tccgatttgg tcaagagatg aagcaaaggc
    55141 aatcaaacca aaacgtgaga cacggccata agtaggtttc atccctacga tgccgttaaa
    55201 agctgctggt tggcggatac ttcccccagt atcactacct aaagaaactg gaatttgtcc
    55261 agctgcaact gctgctgcag aaccaccaga agagccacca ggtacttttg ttgggtccca
    55321 agcatttttt gttttcttga aataagaagt ttctgtactt ccacccatag caaattcatc
    55381 catgtttagc ttaccaacag ggatcatatc tgcttgatag attttatcca tgaccgttgc
    55441 atcataaatt ggttcaaaat tgtaaaggat cttagatgca gcggttgtca agatatcttt
    55501 tgttacgata ttatccttga taccaattgg aataccagca aggacgttgc tttcatcaat
    55561 ccctttttca tctaaagctt ttgcctgttc caatgctttt tcttcgttca atgtaatgaa
    55621 tgcttcgacc tcaggctctg tttctttgat tcgttcaaat gttgcttttg tcaaatcttg
    55681 tacagtgatt tctttcgata caagcatatg atgcagttct tctagagaac gattgtgtaa
    55741 ttctgtcatt atgcgccagc ctccccatta tcgatgattg cgggtacttt gataaagccg
    55801 tcttctgttt ccggcacatt tttcaataat tcatcgcggt tccatccagc ttgcgcccga
    55861 tcttgtcgca tcacgtttac tgtttcaacc acattcgacg taaatggaac accttctgta
    55921 tctacttctt ccagcatttc aaccatatcg atgattttac ctaattggtc ggtaaaactt
    55981 gcaagttctt catctgcgaa cgcaagtttt gctaatttgg caacgtgttt cacttgttct
    56041 tcactaatcg ccatactacc ccttctttct aaacgagtta aaaactttgc tctctatttt
    56101 aacacaagag tgccgtgtct gaaattgttt attgaaattt gtctgaaaaa actcattttt
    56161 cattcttcta ctgagcgact tttagccaat gcatctgcca tttgatcttc tgtccaaact
    56221 tcgattccta attcttgtgc tttggtcagc ttgctgccag catcttctcc tgctaccacg
    56281 atatccgttt ttttcgatac gctaccagtc actttcccgc caagattttc gatcgtctct
    56341 tttgcttctt cacgagtaaa tcgtgttagt ttaccagtaa ggactactgt tttttcttta
    56401 aaaggagact cgacttcttg caattgggca tttcgaagcc ctttgtattc gaaattgacg
    56461 cctgcttgtt ttaattcatt catcaattca tggacttctt cgttactgaa ataagtgact
    56521 acactgtcgg caatgatatc accgatcgta tcaagcgcaa taatcgactc atagtcactt
    56581 ttactcaatg tttctagatc accgaaatgt tctgctaaaa tttttgctgc tttcgctccg
    56641 acatgtcgga ttcctaaccc gaaaatcaac cgctcgaccg aattatcttt gctgttgtcg
    56701 attgctgtca aaatattatt tgctgatttt tcttttattt tgtccaacgt caataactct
    56761 tcttctgtca atttgtaaag atcagcaaca tctgctacta gttttttatc atacatctgt
    56821 tctagtacgc gcggccccaa cccatcgata ttcatcgcat ttcttgaaac aaagtggttc
    56881 aaaccttctt tcatttgagc agggcattta ggattgatgc accgtaaggc tacttcttca
    56941 tccaaatgga caagttcact tccacaaact ggacaatgtg taggcaattg gtattcttcg
    57001 ctgtctttag gtcgtttatc taacacgact tgcgaaactt ctgggatgat gtctcccgct
    57061 ttataaatca gaactgtatc tttcaagcgg atatccttca acttaatata atcaccgtta
    57121 tgcaggctgg cacggcttac tgtcgttcct gctactcgga ctggtgtcat gatagctgtt
    57181 ggcgttacta cccctgttcg accgactgtc cattcgatgt tttcgattag cgtttcaact
    57241 tcttctggcg gaaacttgta agctgctgcc catctcggtg ctttgacagt aaaaccaagt
    57301 tgatcttgta gcgagaattc attcactttg atgacgattc catcgatttc atagggcaaa
    57361 tccacacgtt tgtcatgata ttcttcaatg tatgaccaca cttcatcaat cgaatgacat
    57421 agacgttttt cccgattcgt gtggaatcca attttttcta gctcttctag cgcatcatac
    57481 tgggtttttg cttgcatagg accgaaatca gctactgtgt ataaaaaagt gtccagattt
    57541 cttttcgctg tgattttaga atctaactga cgcaaagaac cagctgcggc gttccgagga
    57601 ttcgcaaaga tatcttttcc ctctgcttct ctgtcttggt tcaattggac aaatgaccgt
    57661 tttggcatga aacattcgcc tcttacttcg atgttcattg gttcttttaa tttgattggg
    57721 accgaccgga cagtttttag gttttcagtg atgttttctc ctacagttcc atccccgcga
    57781 gtcgcacctc tcacgaaatt tccatcttca taacgcaaag aaacggaaag tccatctatt
    57841 tttagttcac agcaatagtc aactacacgt cccactgctt tttgtacacg ttgatcaaaa
    57901 gctatcagct cttctttgct gaaaacgtcg ttcaaactgt aaagcggaat atcatgagtg
    57961 actttttcaa aaccttctaa tacttttccg cctactcgtt gagtgggaga atcagacgtg
    58021 atcaaatcag gatattgttc ctcaattgct actaattctg cgtattcttt atcataaaca
    58081 tagtcttcca ctgttggttt atcttctacg tagtattcgc gagaccattg gttcagtctt
    58141 gtacgaagtt cttctgctcg tttagcagct tcgtcaatcg tcatttcatg ttccatcatt
    58201 taccctccac tactaagctt tttcaattgg cgcaaaggca gccaaaagac gttttactcc
    58261 ttgacttggg aaagccacat ctagttctag atccttcgac gtcccgctta ctcggacgac
    58321 tgttcctaga ccccattttt tgtgtttgac tttatctcca ggtttccagc tgttattttc
    58381 ccctccagta gcacttttag agggaacaac cgctgtctct ggctgtttat agctatatgc
    58441 tgttttttta gcgaatacag gtgttttttt aggcgtagga cgcatgcctt cgatttccag
    58501 taactcctga tcgatttctt ctacaaaacg agaaggtcgg ttatattgcg tccgtccata
    58561 taatgttctg gagaaagcat ttgttaaata caacgcttct tctgctcgcg tgatccccac
    58621 atatgcgaga cggcgttctt cttccagttc gctttcttcc atcaatgctc gagataaagg
    58681 aaaaaccccc tcttctagac caatcaggaa tacaactggg aattctaatc ctttggctgc
    58741 atgcaaagtc atcaatgtca cttgggaagc atcttcttct aaattgtcaa tatcggaaac
    58801 aagtgccaga tcatttaaga agaccgttag tttttcttct ggtgcatcag cgtcttcttc
    58861 attttgctgt tcgaattgtt tgtcgaattc ttgtgtgacc gttaaaaatt cctccaagtt
    58921 ttcgatgcgt gcttgtgctt ctaatgtatt ttggattttt aagtcttcta gatatccgct
    58981 acgatccaaa acctctttcg tcaattctgt cactgtcaag tatgggatca tctgagtgac
    59041 ttcttggatc atctctccaa atgcacctaa ttgctgtcct gcttttccac cgatatttgc
    59101 cagatccaca ttttgcgctg cttcaagcag tggccattca tgaagagaag caaaactgcg
    59161 tagtttttcg atagatcctg gtccgatgcc tcgtttgggc gaattaacga tacgctcaaa
    59221 actgattgaa tcttgaggat ttgccagcac attcaaatac gctaaaatat ctttgatttc
    59281 tttacgatcg tagaacttgt gccctccaac cattttataa ggaatatttg ctttcaacag
    59341 agtttcttcc attacacgag attgtgcgtt cgtccggtac aagatggcaa agtcgccgta
    59401 attccgatga ttgcttcgaa tctcttcttg catccgatct acgataaaac gggtttcgtc
    59461 tcgttcatta tctgcacggt agtaagtgat cttgtttcct tctttgtttt ctgtccacaa
    59521 gttttttggt tttcgattgc tattgttttc aatgacttgg tttgctgcgc taaggatgtt
    59581 tttcgtggag cgatagtttt gttctagcaa aattactgca gcatctggat aatctttttc
    59641 aaaatccaag atgttctgca tatctgcacc gcgccagccg taaatacttt gatctgcatc
    59701 tcccacgacg cacaggttcc taaatctacc tgccaataaa ttgactagcg tatactgtgc
    59761 gtgatttgta tcttggtact catctacatg aatataatgg aatttgtttt gataatatgt
    59821 gagtgaatca ggatgttctt caaataatcg aatcgtattc atgatcaggt cgtcaaagtc
    59881 catacattga ttatattgaa gctctttttg gtaagcagca taacattttg ccgcaatctc
    59941 ttcgtaataa ctgccttgca tttttgcaaa atcctgaggg gtctgcaggc tgttttttgc
    60001 ttgagagatt gttcctaata tacttcgagg atcatatttt ttcggatcaa tgttcagttc
    60061 ttttagtatc cgtttcatca aggtcagctg ttcagaagaa tcgatgattg taaagttccg
    60121 gttatacccg ataaaatcaa catctcttct taaaatacgc acacacatcg agtgaaaagt
    60181 cgatacccag acatcttcgc ctccagatgc gagaatcgca ttgacacgtt ctttcatttc
    60241 ttttgcagcc ttattggtaa aagtaatcgc taggatgttc caaggattga cttctttttc
    60301 ctcaatcaaa taggcaatgc ggtgtgtcag cactctcgtc tttccacttc ctgcacctgc
    60361 cattagcagt aatggtccgt cagtgtgaag aacagcttct ttctgacggg ggttcatccc
    60421 gtttaataat tcagctttgg atgacattaa caacatcctt tcttttatca atcttgttaa
    60481 ttatagcatt ttcttggctt tcattaaact agctagttca aaatcatttg tttcttttta
    60541 tggatttttc cttgtcaatt cgttgatttc gcgctaaatt aaaagaaagc gaggcgtttg
    60601 aatggaaaaa aaaggaaaaa tcaagaaatt agataagact cagatatatt ttattctaaa
    60661 aggaatattt gttggactgc tgacaggaat cgtcgttagc ctgtttcgat tatctatcga
    60721 aaagctaagc gatttagttc gaagtatcta tgaaatgagt cgggaacagc cgatttactt
    60781 aattggccta gctatttgct gcatccttgc agcatttttc gtgggttatc tggtcaaaaa
    60841 tgaaccggat atcaagggaa gcgggattcc acaagtagag ggacagcttc gcggggaatt
    60901 atcaatgaac tggttctctg ttttatggaa aaagttcatt ggcggtgtac tttcagttgg
    60961 tgcgggcctt tttctaggta gagaaggacc ttctatacag cttggcgcat ccattggaca
    61021 aggagctgga caattgtttc ggagtccatc ttcagaagaa aagattttaa tttctagcgg
    61081 tgcaagtagc ggacttgcag cagcattcaa tgctcctatc gcaggattac tctttgtttt
    61141 agaagaaatc catcactcct tttctccact tgtttggctg acatcatttg cttcagcaat
    61201 cactgctaac ttcgtctctc tttacttttt cggcttgaga cctgttttat atctaggtga
    61261 ccttccttct ttgccgttga aatattatgg ttccttagtg atcttaggag tacttctagg
    61321 catcttaggt tttatttatc agaaggtctt gctggctttg ccaaaatttt acaaccgatt
    61381 gcctttacca gcttattttc acgggttcgt tcctttcttg ctgattttgc caattggtta
    61441 tttatggcct agtactctag gcggaggcaa tcaaatcgtt ttagcttttg gacatgcttc
    61501 cttgccaatc attatgatta tcggtttgtt tgttcttcga ttcgtttttt ccatgatttc
    61561 ttacggtggt ggtcttccag gggggatttt ccttccgata ttgacattgg gggctttaat
    61621 cggtagcttt tatggaaaca ttatggttga tttcgggatg gatccaatct acatcaagga
    61681 ttttgtcgtt attgccatgg ctggttattt cacagcgatt ggaaaagcac cgctgacagc
    61741 aattatttta gtgacagaaa tggttggctc tcttaatcat ctgatgcctt taggattggc
    61801 tgctcttgtt gcttatatcg tcaatgacct tctcggcggt aatccaattt acgaatcatt
    61861 attggaacgt ctacttggaa atactcagac cgcctcacta aaaggtgcaa ggacaacttt
    61921 tgattttcca gtaacggcag agagttcttt agacggatta atggtacgag acttcaattg
    61981 gcctaaaaat atgctgctaa tctctatacg cagaggaaat caggaaatct tgactcacgg
    62041 tgatacgatc atgaatgtag gagatatttt ggttatcttg actgatgaag caaacttgcc
    62101 aagaatcaag caggaaatcg aggctaaatc ttctatcaca aatttgaagc aatcatttta
    62161 acgaacatgg aaaaactatt ttacaacaac tattcatcta cactacttga ttctatatga
    62221 ttctataaaa aaagaagaga ctgggaaaaa ccagcctctt cttttttatt tctatgcttc
    62281 tgctgctgca ggagcttttc tcaataaacc ataaatcaca ccagatacga ctgctccgat
    62341 agcaataaac accaaataca tgaccgggtg actcagtaag aatacaacga aaatcccgcc
    62401 atgaggagcc agtaatttga tcccgaaagc tccgactaat ccgccagtta aagcagaacc
    62461 aataataaaa cttggaatgg cacgaatcgg gtcagctgct gcaaatggaa tcgatccttc
    62521 cgtgacaaac gatagtccca tcacgatgtt cgtcaaacca gcatcttgat ctgttttgct
    62581 gaatttattc ttaaacaaac gtgtagcaac gaaaattgcc aatggaggta ccattcctcc
    62641 agccataaca gaagccatca cgacacttcc gccagttgca acagtcgtag ctaaagttgc
    62701 tgtaccaaac acataagcgg ctttgttgat tggaccgcct aaatctgccg ccatcattcc
    62761 agctaataat gcaccaagta aagcagcatt cgaaccactc atacttgtca agaaaccgtt
    62821 caatgcatca ttgattgctt tcatagggac attgaccagc aacatcaaga aaccagtaat
    62881 cagtaagcca aacactggat aaaagaggat cgttttaatt ccatccaatg attttggtaa
    62941 gttcttgaat aatttacgta aaaatacgat gacataacca gctaagaatc caccaatcag
    63001 tgcacctaag aatcctgcac cgcctgtatt agctaatgca ccagcagcaa atccaacgat
    63061 caaaccaggg cggtcaccga tacttgaagc aataaatcct gctaataccg gcagcatgaa
    63121 accaaatgca gcttgtccga tttggttgaa ccagctagca gcttgattgt aattccctag
    63181 atttgataat tgatcatgtg gcacgccaat aaattggtcg atcatgaaag aaagcgcaat
    63241 agcgattcct ccaccaataa cgaaaggtaa catgtgtgat acgccgttca tcaaatcttt
    63301 gtaaattctt tgtccgatag taccatccgc atttccttct tctgaactgc tttcttttcc
    63361 atcaccatgg aatactggtg cagtaccact aagggctaga ttgatcagct cttctggttt
    63421 acggatacca tcactgacag gacgattgat cagttctttt ccgtcaaagc gattcatctc
    63481 tacttttttg tctgctgcaa cgattacacc ttctgcacgg gcgatgtctt cagcagtcaa
    63541 tcggtttttg attccttctg aaccgttcgt ttcgactttg atctccacac ccatttcttt
    63601 cgcttttttc ttcagagcat cttctgccat gtaagtgtga gcgatccctg ttggacaagc
    63661 tgtaacggca acaacgaatt ttctttctgg atgagcagaa gcttctgcag cttgtgtttc
    63721 ttgttctgct ttttcttctg cttctttttg ctcttcagct gtttggaaca agtcttggac
    63781 ttcttctggt gtatttgctt cttttaattt tcctacgaaa tctgcatcaa tcagcaatcg
    63841 agatagtgct gctaatgctt gcaaatgtgt gtcgtttgcg ccttctggag ctgcgatcat
    63901 gaaaaacaga taagttggct gtccgtctaa ggcttcataa tctactcctt tttggctttt
    63961 tgcgaataaa actgttgctt ctttaactgc actgtttttc gcgtgaggca tggcgattcc
    64021 atcaccaaga cctgtagaag tttgtgcttc gcgggctaga attccttctt tatatgtttc
    64081 gatatcggaa atccggccgg catcaaacat tttttggacc atttcatcga ttgcgccttt
    64141 tttatcagtc gcttgcaaat ccatgatcat cgcttctttg accattaaat ccttgatatt
    64201 catgattatc ctccttgaaa ctaatcaatt tttgtgattt caacttctgg cagcaactca
    64261 tcaatcaatt cgcgagctgc caagtcatca gaaaaagctg ttgcgcttcc gcaagcaact
    64321 ccccatttaa aggcttcgat tggatctttc gtacggctgt aagccccaat aaagccggca
    64381 atcattgaat cccctgcacc tactgaattt ttcaatgggc gaactaatac attcgtttta
    64441 taagttcctt gttccgtgaa gagcaaagca ccgtctccag ccattgagat cagcacattc
    64501 tgtgcccctt cagctagcaa ttttttacca tacggaatga tttcttctac agattggaag
    64561 gttgtattgt aaagctcagc taattcgtga ttgtttggtt tgatcaacaa tggtttctta
    64621 acaagtgcat tttttaggtc atcacctgtt gtatcaatca cgaaatctgc acctttttct
    64681 cgaatgatat caatcagttc ttgataaaat ccttttcgca ggctagccgg cgcgcttcct
    64741 gacaatacga cgatatctcc ttgtcctact gttcctaatg cttgcttcaa ctcatcgatt
    64801 tcttcatctg aaatagatgg gcccaaaccg ttaatttcag tttcagtatc tgattttagt
    64861 ttgatattga ttcttgtatc ttcttttacg gttgtgaaca aagactggat ttgttctttt
    64921 tcaagccact tcgaaataaa ttcgccagtg aaccctccta aaaaccctag tgctgtagat
    64981 tgagaatcga tccgtttcaa aatacgggaa acattgatcc cttttcctcc aggtaacttg
    65041 aagtcttcct tcattcggtt caaatcgcct agttttaacc catctacgcg tacgatataa
    65101 tcaattgatg gattaagtgt tactgtgtaa atcattgtat tgcctcctta attgttgtct
    65161 tttcacggaa ttcattaacg aattctaatg gacaacgctc ggttatgatc gtggctgctt
    65221 ccaaaggtgc aacagaggtg aaggacaact ggttgaattt ggtatgatca atcaatacaa
    65281 atgtctcttc ggaatttttc atggctaaac gtttaacagc tgcctcttct gggtccggag
    65341 tcgtgaatcc ttgttcaaga tgggcaccgt tcattcccat aaatgcttta ttaaaatgaa
    65401 aattttttag ttgttctatc gcattagatc ctaaaacagc attagttgcc agctttatcg
    65461 ttccacctaa aataatggta gcaagctcca aatcaactaa atttgccgca tgttgaacgg
    65521 aatttgtcac tactgtgatc tgttttcctt gcagaaaagg aatcatttct aacgtagtag
    65581 agccagcgtc taaataaatg acatcttcgt cattaatggt ttcagctgcc gatttggcga
    65641 tttgctgttt ttcgtgaacg tttttgagtg atttttcact catattctgc tcaaaattca
    65701 ataattgctc tttcttagct ccgccatgaa ctctttctag aaacccacta ttctccagct
    65761 cttgtaaatc acgtcttatc gttgattcag aagcattaaa aaggtttaca agatcttgtg
    65821 atttcacgat tttctgctgt tcaagcaagt ctaggatttt ttgtctacgt tcctctgtaa
    65881 gcattttcac gatcacctca atacaaataa tagcatattt tttttctact cgcaatataa
    65941 atcagtcaaa aacgttcata aaaattataa atcagtcaaa aaaaaataaa aaaccagtca
    66001 aaaagactgg ttttttattt ttttgtctat catcaagccg ttttatcgtt ttttattttc
    66061 gcttcttgtt tttttcatat aaacagaagc aggttttctt tccttatgaa gaagataata
    66121 ataaagtccc atcccgccaa acatgaccaa aaaaccgatg actgatcgaa taggaaaatt
    66181 aggaacaaaa aagagacaga cggataataa tgatgccgga accattattc gataatagag
    66241 ttgtttatcc attttcttca cctcatattt tttgtaaagt aatcaattgt tctaattctc
    66301 ggtaatctaa aaattgcaca cgcagatgct gctcaccttt cttgacatcg aattggatac
    66361 ttcgccgctt tttctttatt cgtttgaagg taacatcaga gagtctatgt ttgatttcct
    66421 ttaacggtaa ttctgacagg gattctgaag gaacatagat tcttaaggat aaaagatact
    66481 cttgatattt ttcatctgtt acctctactt ctagatattc cctaatataa taacaataag
    66541 cttcgattcc attatccaaa gcaacagtcg gatcgataac tttatagcac atcatgcgcg
    66601 agcgattctc gtcaacccaa ggaaaatacg cctgagcatt tagtgttccg cgaacacgcc
    66661 tttctttata atctccttgt tgaatatcat aactttttgt tacaaaaaat gaagcattct
    66721 ttttaggaac aggttgcatt tgtgcattct gatactggac agattggatc gtatggtcag
    66781 tgtaaatgta ttcaacgaat ggcggtttca ataaaccgac accattgtcc caagcaagat
    66841 gatcaaaccc aagctggtcc ctaaaatagg attgaaacat ttgattgttt ggcttttgcc
    66901 agttaaaggg gtctcgataa agctggatac atatgacttc gttttctaat tccttcactt
    66961 tttctacttg ttggatgtcg agaaaaatcg gtttagggat gatttgatga taatagcgac
    67021 tataatacat ttcccaacac gaagtgaaac ataaaccttg gtaaaaaaga tcgtatcctg
    67081 gaaattggtt gcaatcgact actacttccc cgttttgttg tttcatttta ggtaaatgtt
    67141 cgatttcatc ctttttctca aacaatagcc gttgagagat ctgttttgta ttgtgataga
    67201 taaactcgct ataagctcgc atataggcga acaccccatg agcaatcatt tttttctgaa
    67261 tgaagtctcg aatcaaaaat tgcttttctt tgaaaacaga gatagacaat acatttttta
    67321 tcaataattg attatcttcc aagcgaaaac tacattcgtt ctcgtcattt cctacaacta
    67381 accaactttc gtctcttacc gatgctagca ttattttttc ttgaagcttt cgaagatcat
    67441 acggatccgc ttgttgtgtg aaagagcgga taaatgttgg ctggatcaac agacgtgtca
    67501 aaatagccca atcgcttatt tcctgttttt tttcttcttc atttaagtct gtgagctgga
    67561 tagtcatcaa taatttatct tcgccaatca ttttctcacc tctttaaaat ctttttataa
    67621 tttaattata accaacgata aagaaaaact aaaactttat gtcttagaaa ccaatttgga
    67681 cgattacatg atagactaag taaaaagaaa caaagaggtg tttacaatgg ggttgaaatt
    67741 tgaacgtaca gataatttag ataaactttt cgaatcattt gctgtcgatc cggaaaaaac
    67801 agttgcacca aaagaagaga tcgatcgatt tctaaaacca cagccacaaa atgaaaagaa
    67861 aaaagacaag aaacaaaagt aagttaagct caaaaaaaga cttaccagcc aaacttttat
    67921 gaaagagttt gactggtaag tcttttttat tctttttatg attcaattgc ttgttcgatt
    67981 gctgcccacg cttcttcttt tccctgtttt gtaacggaag aaaagagaat aaacgtgtca
    68041 ttgcgatcaa aatccaacgc ttttttgatt tcgctctcat gcttgttcca tttaccacga
    68101 gggatcttat ctgccttagt ggcaacaaca attacaggga tatcataata cttcaaaaat
    68161 tgatacatct ggatatcttc ttgagaaggc ttatgacgca aatctactaa agaaacgacc
    68221 gcacgtagct ggtctctagt cgttaaatat gtctcgatca tctttcccca ttttgctcgc
    68281 tcagttttgg atacttttgc atacccatat cccggaacat cgacaaaatg taatgcgttc
    68341 tcgatcaaat aaaaattcaa tgtctgggtc tttcctggct taccggaagt acgggctaaa
    68401 ttctttcgat caatcaatgt gttgataaaa gaagatttcc ctacatttga tcgtcctgcc
    68461 agagctattt ctggcaattg tgtatctgga tattgttttg gtgccactgc acttatgata
    68521 atttcagctt gatggacttt catggtctct ctccttaaca aggtgatgaa taaagcaagc
    68581 ggctgtcgca aaagccaaaa tgacttttac ggcagcctct tttcatcaac ccgctttttt
    68641 ctctgaatct ttatagatta ctgttggctc accagacaat tcagctgctt cttttgtgat
    68701 aatcactttt tcgatgtttt catttgaagg gatatcaaac atcacatcca tcatgatttc
    68761 ttcaatgatg gaacgaagtc ctcgagcacc ggtattgcgt tcgattgctt ttttcgcaat
    68821 ggctctcaac gcttcaggtt cgaattcaag ttcggtatta tctagagaaa gaagtttttg
    68881 atattgtttg actagcgcat ttttcggctc agtcaagatc cgtaccagat catctgttgt
    68941 caatttctct aaagctgcca taacaggtaa tcgtccgata aactcaggga tcaatccgaa
    69001 ttttagtaaa tcttcaggaa tgatatgctg catgacactt tcgtcttcag aaagtttttg
    69061 gttttgtgta ccaaatccaa tcgtcttttc gcccatacga ttcttcacaa tcgtttcgat
    69121 gccatcaaat gcaccaccta cgatgaacaa tatgttcgtc gtatcaatct gaataaattc
    69181 ttgatgtgga tgtttacgtc ctccctgagg tggcacgctt gcaacagttc cttctagaat
    69241 tttcagcaag gcttgttgga caccttcgcc ggaaacgtca cgagtaatag atacattttc
    69301 gcttttacgc gcaattttat cgatctcatc aatatagatg atcccttttt ctgcacgttc
    69361 cacattgtaa tcagcagatt gaagcaattt aagcaaaatg ttttctacat cttcccccac
    69421 ataaccagct tctgtcaaac ttgtcgcatc tgcgattgca aaaggtacat tcaatgtttt
    69481 tgctaatgtt tgtgctaaaa atgttttacc agaccctgta ggtccgatca agcagatatt
    69541 gcttttttgc agttcaacat catctgtgtt ttcttgtgct tgctgattga cacgtttata
    69601 gtgattgtat acggcaacag ctaacgagcg ttttgcgcga tcttgtccaa tcacgtagtt
    69661 attcaatact tcaagaattt ctagtggttt agggacttca gtgaattcgc gtacagcttc
    69721 ttcataaaat tcttcatcaa tgatttcttt acataggtcg atacattcat tacagatgta
    69781 gacccctgga ccagctacga tctttttcac ttcttcttgg gttttcccgc aaaaagaaca
    69841 gcgtacggtc tcattgctac ttggattgtc atacatggtc ttcacccctt aacttgcaaa
    69901 aagtatgcaa gaaactatca aagactttac agtcttttac tatcataaca tagtttagta
    69961 aaaagaaaaa gtgtttaaca aaggatcaac tgacctatac ctttgcattt tttactctta
    70021 acaaaaaata aggaactgtg acaaaatcgt gtcacagtcc cttatttcga ataaacaatt
    70081 ttctagaagt agcttcttca gaaatgattt attacttgat ttctattctt tcgcttctgt
    70141 cgctgtacct gtgatcaatt cgattgcttt tttcattgtc acatcatgtt ccagcatatc
    70201 ttctgtcaac acgcgtttga tttgttcgat tggcatgttg tatgtttctg ataattcttg
    70261 gatttctgca ttgatttctt cttctgttgc ttgaagattt tcagcagctg caattgcttc
    70321 gatcacaaga tttgttcttg tgcgcatttc tgcttcacct tcgaattgtt tatgcaagtc
    70381 ttcttctgta gaaccagtta attgatagta catttcagga gagatacctt gacgttgcat
    70441 gttgttcaag aattcgtcca ttgaacgatg tacttcatca tgaaccatca cgtgtggcaa
    70501 ttcaacgatt tctgcgtttt caacagccat acggatcgct gcttcatctt ttgcatcgtc
    70561 cgctgctttt tcttttgttt ctactaattc tttgcgatat ttttctttca attcgtctaa
    70621 tgttgatact tcgtcatcaa catcttttgc gaattcgtca tctaattcag gcacttcttt
    70681 tgcttttact tcatggattg tcactttgaa gactgcttct tttccagcaa gatcttctgc
    70741 ttgataatct tctgggaaag ttacatttac atctagactt tcacctgatt tatgaccaac
    70801 taattgatct tcaaaacctg gaatgaatga gcctgaacct aattcaagtg agtaattttc
    70861 gccttttccg ccatcaaacg cttcgccatc tacaaagcct tcgaagtcga ttactactgt
    70921 atcgccattt tcagctgcag catcttcttt gataactaat tcagcttgtg cttcttgttc
    70981 acgtttgata cgtgcatcca cgtcagcatc tgttacttca cggtcttgtt tttcaacaac
    71041 tagatcttta tattgtccta attttacttc tggtttcact gttacttcag cagtgattac
    71101 ccagtcttga cctttttcca tgctttcaac atcgattttt ggttgagaaa caggatcgat
    71161 gccagcttct ttgacagcag cttcataagc ttctggtaat acagcattca atgcatcttc
    71221 atataaagct tcttcaccgt acatacggtt gaatacttga cgagaaactt tccctttacg
    71281 gaatccaggt acgttcaagc tttttttcac tttattaaac gcagttgtta atcctttttg
    71341 gatttcgtct tgcgcaatcg taaattttaa aacaccatca ttcgtgcctt ttttttcaaa
    71401 tgttgcagtc atttaattcc ctccgagttt agaaattcat tttatacatc aaaaacgttt
    71461 catgcatacc tttaaatagt acactaaccc tttcaaattg taaacatatt gaatggatag
    71521 aatccttgtt tttcataaca tttttatctt tttcactgat aaaatctaat ggaagaagat
    71581 catttgctgc aaaatttttt ctcttttttt taaaaaatct tgtttttcat cttctgtacc
    71641 ttcatacatc gtttttcctg tacacgcctc tacagtcagt tgaaaccagc gcttcgggtc
    71701 ttcgatgtat gtatcataga caggataaac aattgcagat tgcaacgtga attcttcatt
    71761 taacacggaa ctaagcagcg catcttcatt accgtaacga tctgaaaata cacataattg
    71821 ctggtaactt gcctgctgct gcggctttgg cagatctttt ggtatgactt tgactaactt
    71881 ttcttcaatc gtcaaataga aaacaggatg gcattcgttc aattgcctaa gtgtctccag
    71941 taattttgcc cttgctaaaa tatggacctc tggcgataca ataagtttac tcgctgtttg
    72001 gataaactca tgataaggaa gctgttcgat ctcatcaata gacgcaagct gacgtgcagg
    72061 ttccaacgaa ggaagttcct ccgatttatt gtggagtgcc ttgatcttct gcctctccat
    72121 ttgtccggat aattcttctg caaattggat tttttcaatg acccgctgct cgaaagcttc
    72181 ataaaaatct gtacttgcta tcaattttct agcatataaa aaatcttgtg tactgattaa
    72241 caagtcaaaa taaaattcag caaactcctc attttgaaga tactgttttt catgcaagag
    72301 tgcttgcttc aatgcttctt ttttctcgtc caactcgtaa agacaaaaaa caagcaggct
    72361 attagcctca aagctttctt tcaactcgta agctcgttga aataattctt ttgcctgctg
    72421 atattgctgg tttgctagtg cttgttgtgc tactcgcatt aaattctcat atttttctgg
    72481 aaaatcaatc ggcttcacgc agtgtcctcc tcttgctcat gttcagctca tgttcaatag
    72541 tagcaaaaca aaaaaagcat gacaattaaa aataactgaa gcaccctcta aaagctagat
    72601 tttagatctg acttttagaa ggtgctttca actgttactg ctttttattg ctcatcagct
    72661 gttgacttcc tggttgaatg ctttgatcag attgatcgta acaacacttg tattagtgaa
    72721 aaaatcgcct agatgttgct ttttctgatt aaagattgct ggaagatacc ctaacatcag
    72781 caaaggaaat tttagaatga accggcaagc accttctcta actaaaacgg ttgaccagct
    72841 aagttcttct tcatcaaagc tgatgactcg aataccaaag atcattttcc caatcgtttg
    72901 accatgattg aatttcgtca ataaaataaa atacgctaga taaataagta aggctgtgaa
    72961 tccgtagata ctcagataag aatctgaaaa attaagccaa cctagatttt tggcaatacc
    73021 aagtgttaaa cgcgtaatag ctccgataca caaaagatcg accaagaaag caaacattcg
    73081 gataaaaaaa cctgcataga aatacttagg aaaatcattt gcttttggcg gttcatttct
    73141 tgtgtccgta tactttttcc atttacgttg tttctttttg atttcttcgg gcgtcaaagg
    73201 ctctctttca aaagagctca gtaccttttg ggaaaagttg gagtcaggtc ctacagcagg
    73261 ctgctgttgc ttatcttttt cttgttcctt attttcattt gaaagctctg acatgcttac
    73321 tcacctccgt aataatacat tgcttttggt gattccgctg tccctagatt ctcaagaacg
    73381 gataagatct gactagtctc agaaggcttc aatccctgga attctgctag tttacttcct
    73441 aaccatgtac tagcaaaccc agatggcgtg gaagaatatt ctactagttc cgcatcttca
    73501 agctgttgtt cctttcgcat agcttttaaa gcatcttcag gaaatcccaa ttcatcgact
    73561 aaacctactt cttttgcttg gacaccatca taaatccttc catcagcaat ttttttgact
    73621 tcttcttcag atttattccg tccctcgctg acgattgcta caaaacgact atatgcgctg
    73681 tcgatatatg cttgaagcac cgcttcttct tcttttgtct ctggtcgcgt tcctgaaccc
    73741 atatctttca acgctccgct tttaactgtt gtatcttcca cacccagttt ttctagaagt
    73801 cctgaataat tcaatcctga catgatcaca ccaattgagc cggtcactgt ttcttctgtt
    73861 gcaaagattt tatctgcttg agcagaaata tagtagccac cgctagctgc catgtttttc
    73921 atgctgacat acattgggat atcatgttct ttacgaatcg tatccaattt cttagcgatt
    73981 tccgcacttt cgtagacacc gccaccagga gaatttactt ctaaaaagat acctttgact
    74041 gttggatctt cttcaatcgc cttcagttgt tccataaaat ttttatgatt gtaaccttca
    74101 gaagcaaata aacctgaaga ccctcccgca gcaatcgttc catctactga taattttacg
    74161 attttttgcg tactgtctcc ctcttccaac acatttgaag ctagttcttc atccccatat
    74221 aaccaactgt tcaatccctc aatttgatcg ttttcctgag gcttagtcaa actagctgaa
    74281 actaacgaaa ccacaaataa tcctgcagca atcagaactg ctgcccaacg tctcttattc
    74341 attctttctc ctttctaata caagtttttt atgacgatca cagtttctga ctgctcagaa
    74401 gtgtcgaacc gctctgcttt ttcttcccgc gcatacccgg aaaacgactc ttttgtcaat
    74461 tcatcttcta ccgtaaactc acgagcttca gcaacttcca aggcatcagg tgcgatttct
    74521 tggatgctat cccagccagt atctaaaaat attttcgtat catctgatac accagctgat
    74581 gcttcgaatt tggacaattg tttttttaac attcccagtg tcatgatctc aatttttgaa
    74641 tccattttta ctcctcctct atccgattac tttctattgt accgaactcc tgaaaaagat
    74701 gatagataaa aacccattat tttttgacat aaaaagagca tttgacaaag catttgatat
    74761 ataccaattt attttttatt ctttttttgt tgacaagaac attcgcaaaa ggttatgatt
    74821 ttaaatgttt ccagataaaa aacaaaagag gaggatagtt atacctttta ggatagcgcc
    74881 aggtctgtgt aaacagatga cgaggagagg gtttatcgaa agattcggcg gatggcccta
    74941 gggactgcac tctacagaaa atccacaaaa aatatctgca aagataaaac aaaaggattt
    75001 tcctgcagct ggaagcattc atacgaattc attatagaaa gtaggatgtt ttttatgtgc
    75061 ggaattgttg gaatgattgg attaaaaaat gtaacaccag gattgattga cggtctagaa
    75121 aaattagaat accgtggtta tgattcagct ggtatctttg tctcagatgg cacaactgat
    75181 tatctggtca aagcacaagg cagaattcaa aatttgaaaa acaaaataaa tattgatact
    75241 acaggaaata taggtattgg tcatacacgc tgggcaactc acgggcaacc ttctgaagaa
    75301 aacgcccatc cacatacttc ccaaagtgga cgcttcgtgc tcgttcacaa tggcgtaatc
    75361 gaaaactttg aagaacttaa aattgcttat ttagcaaatg accactttat tggtgagacc
    75421 gatactgaga ttatcgctca tctaattgaa actttcgcga aggatactac aacgcaagag
    75481 gcgtttttaa aagctttgcg ggtcatcaaa ggttcttacg ctttcgcttt gattgatcgg
    75541 acagcacctg atgttattta cgtcgctaaa aataaaagtc cactattaat cggactcggc
    75601 gatggattca atgtgattgc cagcgatgca atggctatgc ttgctcatac gaaggaattc
    75661 gttgagattg aagatgaaga gatggttacc gtcacatcag aaaaagttat tattcaaaat
    75721 tttgcaggtg acatcgtaga acgttcttca ttcgaagcac aagtagattc ttcagatatc
    75781 gagaaaggta cctatccttt ttatatgctg aaagaaatcg acgaacagcc tattatcatg
    75841 cgtcgtatcg ctcaaaaata cgtaacagaa gacaatcgtg tctctcttga tcaaaagctg
    75901 gtcgatacat tgtctgatag cgatcgaatc tatatcgttg cttgcggaac tagttaccat
    75961 gcggggttag caggtaaaca gactctggaa aaattgacac agatccctgt tgaggttcat
    76021 ttagccagtg aatttggcta taacacacct cttctttcaa aaaaaccatt ctttattttc
    76081 ttgagtcaaa gtggcgaaac agcagatagc cgccaagttc tggtaaaaat caaccgtctc
    76141 ggctaccctt cacttacgat tacaaatgtt gctggttcta ctctttcgcg ggaagcatcc
    76201 tttacactgc tattgcatgc tggacccgaa atcgcagtag catcaacgaa agcttatact
    76261 gcacaaatag ctgtactaac tttattagcc aaagctatcg gtgataaaaa agaatttgaa
    76321 acttctcttg tattcgatat cactcatgaa ttgagtttag ttgctaatgc gatggaatcg
    76381 gtcatcgccc aaaaagatta tttagaagag ttggcagaag tatatctcag cgatacaaga
    76441 aatgcctttt acattggacg tggcgctgac tacaacgttt ctcttgaagc tgctttgaaa
    76501 ttgaaagaaa tcagttatat ccaggcagaa ggttttgccg ctggcgaatt gaaacatgga
    76561 acgattgctt tgatcgaaga aggaacacca gtgattggaa tcatcagtga agaagtgact
    76621 ggtgcgcata ctcgaggaaa cctgaaagaa gtcgaaagcc gtggcgctaa aacactagtt
    76681 attgtctcag agggattaga aaaggaaagt gaccagctgg tgctaccaac tgtccaccct
    76741 tatctaacta ctctaaccac agtcgtaccg actcaattac ttgcatatta cgcaacgcta
    76801 ttacggggat tcgacgttga caaaccacgt aatttagcga aatcagtgac agtagaataa
    76861 taaaagaaca aggaccagtg tcagacatct gacactggtc cttgttcttt tattttttat
    76921 gaaaatatct cttaatccag tccgatttct tctttgacta ctgccgcgat tttatcgaca
    76981 tagtaattaa ctttctcatc agtaggcgct tctgccatca ctcgaagtaa tggttctgta
    77041 ccggaaggac gaactaggat tcttccttct gatcccattt cagcttctgc ctcttcaatc
    77101 acttttttga ttgccggaac atccatcgca ccgtttttat cacttactcg gatattcact
    77161 aatttttgcg gatagattgt tacctctgct gcaagctctg ataatttctt acctgtctgt
    77221 ttcatgatat tcagtaactg aattcctgaa agcatcccat ctccagttgt gttaaaatct
    77281 aagaaaatca tgtgcccaga ctgctcgccg ccaaagttat aatcgttttt gcgcatttct
    77341 tctacaacgt aacgatcacc aacttgtgta acgacatctt tcaaaccgat tccttccacc
    77401 gctttgtgga atcctaagtt actcatgaca gtcgtaacga tcgtatcttt tttcaatcga
    77461 tttttttctg ccaagtatct agcacagata aacatgattt tatcaccgtc gacaatgttt
    77521 ccaagttcat ccaccgcaat aatacgatcg ccatcgccat cgaaagctag tccagcatct
    77581 gctccttttt caacgaccat ctcagctaaa cgttctggat gggtagagcc tacgccatca
    77641 ttgatattta acccgtctgg atttgtgccc atggtataaa aatctgtttc aagatcggca
    77701 aacaaacgat taacacttgt tgaagttgct ccgtttgctg catcgacaca aatggtcaaa
    77761 ccggaaaggt caccgttgat cgtttgtaca aggaattgag aatatttcaa taagccttct
    77821 ggaaattctt ctacagttcc caatccttca gctgatggac gaggcaattc atccacttca
    77881 gcatctaata atgcttcaat ttctagctct tggtcatcta ctagtttaaa accatctgca
    77941 ccgaaaaatt tgattccatt atcttgtgct ggattatgag aagcagaaat catgacccct
    78001 gcgcttgctt tttgcaaacg tgtcagataa gcaacacctg gtgtagagat cacacctagc
    78061 tggaacactt cgatacccac cgataaaagt cctgaaatca gcgcttcttc taacatttga
    78121 cctgagattc ttgtatcccg ccctactaac acgcgtgggc gttttccttc ttcttcatgc
    78181 tggcttagga cataacctcc gcaccgtccc agcttaaatg caagttctgg cgtaagttcc
    78241 ttgttggctt caccgcgtac gccgtctgtt ccaaaatatt tacccatagt tgtaaaatta
    78301 ctcctttaac ttaattttct gaggaatttg ttctgctcat ttgtgttgat tcgcttgttt
    78361 ctgatgtttc ttctgtactt gtggtcgagc tgcttgtccc agattccgaa ctggaagaaa
    78421 tggttgttgt cgatggttcc gttcctgaat cagcagatga aatacttcta ctcgtacttg
    78481 tattttcttg tgtccgactg ctcgtcgaag cagagctact gcttgacgtt tgcaacacgg
    78541 gtgtaatctc cacactaact gattcgggct tgatacttaa cccctcttct actggtattt
    78601 caattttacg agttgttcga tgattgatac ctgagacatt gatcggtaca gcaatactgt
    78661 caccgatcga tgcaagcaaa tcgctagagc cgcttacttc tgcttccagt tttgaaaaac
    78721 tgaaatcata gctttctacc ccggcagctg gtgttccttg ctgtatccca taaagtcgta
    78781 ccgacttgct tggcttgatc gaatcagctc tcactgttac ttgcactgga tcagagacga
    78841 tacttaatac ttcccctgct gcattcagcg cctgtattgg gacctcacta ttaatcccat
    78901 catctgtgac catcgaagcg tccacatcag ctactactcg tttgatttct gccaatgttt
    78961 tgtcacctgt agtgatagag acttttttag gactaacgga agtatcatca agttcatatc
    79021 cagatggtgt gaacgttgaa ggaatcaacg tttccacatc gaaatctttt attacctttt
    79081 tctcaaccgt tactgtaata gtcgatggtt cgatctttgc tgtgacagca ctgctcaaat
    79141 tttgaatttt tagcggaacc tcatgtgttc cttcgcccag cttagtgata tctgctgtga
    79201 cgcggaacat ccgtgtatct tcgtttgcct ctgcattcaa ctgaactcgg tttgcactgg
    79261 aaagtttgac agtcactgca ttttcatatc catggatata gtagcgcttt gaatcataag
    79321 aaatcttgat tggcacccca gtaactgttt gattatacgc ttcatttcca gaaagcgtat
    79381 ttggaacgga ctggccattt gcattgaagt acagcgcaat actgaacagc aatgcaataa
    79441 aactgtaaag aatgcttgtc cgtttctctt ttgagatcat ttgcgatccc ctcccttagt
    79501 cataccgtct aggaaagatt gaatcacgtt tttcttgttt tttgttttct cttcagcaat
    79561 cagttctcta cgcaaaattg ccaaatattc ttgctgggat aagccagcca tcagttcatt
    79621 atctaatgta atgctgacat ccccagtttc ttcagagaca acgatggtaa tcgcgtcgct
    79681 gacttcgctt ataccgacag ctgcccggtg tcgagtcccg aattcttttg gaatcaacat
    79741 actttctgat agaggaagat aggcgcttgc tactgcgatc tttccttctt ttacaataac
    79801 tgcaccgtca tgcagcggtg tattaggaat aaagatattg atcagcagct ctccagtgat
    79861 atctgcatct aatgcaattc ctgtctcaat atactcatct aatccagtat gccgttcgat
    79921 agtaattagc gcaccgattt ttcgcttgga catatattgg atggctttgt caaaagacaa
    79981 gatcatcttc tcatcttcct gctgttcgct ttttgattgt ttgaaaagcg aactgcgtcc
    80041 aagatgttcc agtcctctcc ggatttccgg ctgaaaaatc acgatagcag ctatcacccc
    80101 gtacgtaatg acttggttca tcagccaaga caaggtgtgc agaccaatga tttccgctac
    80161 gattcggata acaataaaaa tagctacacc tttcagaagc tgaatcgctt ttgtgcctcg
    80221 cagcagttgg atcagtttgt atacaagata ccagacaacc agaatatcaa gtatgttcac
    80281 caaaaaattc agagagaaca agtcaccgga aaacatttgc tgccaatagt ccaaattaaa
    80341 aagttccccg atttgaaatg acataaaaag cctctcttct cttttgagat ttcctctcta
    80401 gtatatcata tgttttaatt ttagaggctg atctgctcgt atttttcttg agaaaaacgc
    80461 ataattttca ggaaaattgc aagtatgcgt tttatttttc tctgcaataa atatttttaa
    80521 acttcagaga attattcatt atattttttc tgttattttt cctttttttc ttttgtagct
    80581 tgttccattt ttttacgtcg ttctgctctg gcagctcttc tttcttctgc ttcaaggtcc
    80641 gtcgtttttt cttttctagt cttaacgggt acatcgatag attcctctgg ttcaagtcgt
    80701 ttcttgattt tttctaattc tcccgtcagt gcagcatagt tgtaaaaacg tgttttagcg
    80761 tcttggatag tcttttcaaa taaagctttt gcaagatttg gatttgcgtt ctctagcgag
    80821 gaaaagcgaa cttgtttcct catgaaatca ggcatagcag agaaatcagg ttttttgtag
    80881 tccaaagtca ttgggttctt gtttttttcg accattgcag ggttatatcg atataatgac
    80941 caatagccag aagaaacagc ttcttttgct tctttaagcg attctttcat tccaccaagc
    81001 aaaccatgtg tgatacaagg cgtataggca atgattaaag aaggtccagg atacttttct
    81061 gcttcttcaa aagcctttat cgtctgcatt tgattggcac ctgaagcaat ttgcgccaca
    81121 taaacatttc catatgtcat cgccatcatt cctagatctt tctttgaagc atactttcca
    81181 ctagccgaaa atttagcgat tgccgaagct ggtgttgctt ttgaggtttg accgccagta
    81241 ttggaataga cttcgttatc caaaacaaga atattcacgt ctgctccgct ggcaagtaca
    81301 tgatcgatcc cgccaaaacc aatgtcgtaa gcccatccgt ctccaccaat catccattgg
    81361 ctgtttttta cgaatagatc ctttctcttg tatatatcct ccagttcagg gacaattgat
    81421 cgttcgcttt cgatcgcagc ttgcagtttt gctgctcttt gctgcgttcc ttcgctctca
    81481 aacatgtgta ccaaccagtc ttccatcaat tctcgtagtt cttctgatac ttttggcaat
    81541 acttctttga ttgaacttgc caaagcctcg cgacgagtct gtgccgctag aagcatacca
    81601 taaccaaatt cagcattatc ttccaataaa gaatttgacc atgcaggccc ttgaccacat
    81661 tcattggtcg tataaggaga agcaggagaa gcaccgcccc aaatagaaga acagcctgtc
    81721 gcattcgcga tcatcattcg atcaccgaac atttgtgtca ataattttac ataaggtgtt
    81781 tcgccacagc ctgagcaagc gcccgagaat tcaagaagcg gttgattgaa ttgtgtagac
    81841 aagacagtat tctttccttt gactggattt gctttttgtc ttaatgtcat tgcaaaagcc
    81901 caattcacag cttgttcttt ctgttcttca taaggcttca tcactaaagc tttttctttt
    81961 gcgggacaag cctctacaca cagaccacaa cctgtacagt cttccactga aacttggata
    82021 cggtatttta cgccgtctgc gcctcgcatt tctctcacga taaatccttc tggcgcttct
    82081 tccatttcct cttcatctgc taaaaatgga cggatggcag catgaggaca aacaaaggca
    82141 cattcattac acatcgtaca gcgatcacta atccactcag gtacttctaa agcgacgccc
    82201 cgcttttcca tagaagccgt tcctaaaggc atttcgccag ttgtcattcc atttgctaca
    82261 agtgtaccaa cagagagttg atttccttcc tgtgcattca ctggttcaag tatttcttgt
    82321 atataaccag tgttcctttt attgattttt tcaggcatct ccaacgttct ccaatggtct
    82381 gggatcgcga cttgatgaag cagttcggtc gtccgatcga ttgcttgtat gtttttttct
    82441 acgatagcca ttgatttatg tccatagctt ttcagggctt cttcttttag cagcggaagc
    82501 acctcatcaa aaggaatgat gtcagctaat ttaaaaaaag ccgtttccat tgccgtattg
    82561 atccggcgtc ccaaaccgac ttcttgggcc aatctcatgg cattgatcgt atagaatcta
    82621 atgttatttt cagccaagta acgttttaat tttaagggca gatgcgtttc taattgttcg
    82681 tcgctccaaa gagtattcaa taaaaaaatg ccacctggct tcaatccttt gaccaagtca
    82741 tagctatgca ggtaagctgg tgtgtgacag gcaacaaggt ccgcatgctc gacaagatac
    82801 gctgaacgta tcggcgtatc tccaaaacga agatgagaga cagtcagacc gccagatttt
    82861 ttcgaatcat aataaaaata gccttgcgca tatttatcgg tgtgatctcc aataatcttg
    82921 atagctgatt tatttgctcc aactgttccg tcagatccaa atccccaaaa cttggcctgg
    82981 aatgtttgcg gttctgtcaa atccagactt tcctttggtt ccaaagattg ataagtcaca
    83041 tcatccacga tccctatggt aaaccgtttt ttgcgtatgc ttgggtcttt tttcaattca
    83101 tcaaaaactg ctgagatttg gtcgggagtc acatctttag accccaatcc gtagcgacca
    83161 ccaataacag ccgggcgaag ttcagaatca tataacgcac tttgaacgtc taataagaga
    83221 ggttctccac ctgcccctgg ttctttactt cgatcaagaa cagcaattgt ttttactgta
    83281 tgaggcaact tttgcacgaa tgtttccact ggaaaaggac gataaagatg gatattcaaa
    83341 aaacctgttt tccgtccgtt tgcattcaag taatcaatcg tttgttcaat cgtctgtgca
    83401 actgatccca tagaaacaat gacttcttcc gcatcgtctg ctccgtaata agtcaccaag
    83461 tcataagaag ttcctcgtaa tcggttgatc tctttcatat atttttgaac gatttcaggt
    83521 aatttatcat aataggggtt gatcgtttcc cgttgttgaa aatggatgtc tggattctgg
    83581 ttcgtaccac taacagtcgg atgattggga ttcatccctc gtttacgaaa tgaagctaat
    83641 ttctcctgat tgatcagagg agctaattct tcatattcca aaacttcgat tttttgaatc
    83701 tcgtgactcg tacggaatcc atcaaagaaa ttcaaaaaag gtacttctcc ttcaatagaa
    83761 gcaagatggg caacaggtgc taaatccatc acttcttgta cactgctttc agctaacata
    83821 gcaaatcctg tttgtcttgc agccatcacg tctccgtgat ctccgaaaat attcaacgca
    83881 tttgtcgtca ctgcccgact agcaacatgg aaaacacttg gcagtagttc cccagcaatc
    83941 ttgtacatat tcggtatcat caaaagcagc ccttgagaag cggtatatgt agttgtcagt
    84001 gcaccagttt tcaatgatcc atggaccaca ccagctgctc ctgcttcgga ttgcatttct
    84061 actactttta ctggttgacc gaatacattt tcttttcctg ctgctgacca ttgatctact
    84121 aattcagcca tggtcgaact gggtgttatt ggatatattg cagcaatctc ggtaaatgca
    84181 taagaaatat atgcggctgc cgtattgcca tccattgttt tcatttttcg catgattcat
    84241 catccttgtt catattctct cttattatct gttccattat aacgtaaaac attccatttt
    84301 ttcttttatt tataaaaata tttcacatat gtctatttga attggtcatc taatttttag
    84361 acaaacagtc taaatcgttg cctttttttg tcagacatgt ataaactata agcgaaagag
    84421 gtgttttaac atggatatat cagtgattga tgcaacgaaa acaaatacac aaaaaggaat
    84481 cctttatggt tcatcgaatg ctcctaaaaa aatggtcgaa ttcatcaacc tatcttgtcc
    84541 gtattgccga caatggtttg aagaatctta tgagctatta gaagaagcag tccaaagcgg
    84601 gcaattgcag cgagtgatca aattgttcga taaagaaaaa gaaagcttgc agcgaggaaa
    84661 cgtgatgcac cggtatctca caatcagcga tggtcaaaaa gcgatcaaag agatcaaaca
    84721 aatattcgat actcaagacg agtggaaaca tctttctctc caagaagtag cggattttgc
    84781 agtagataaa ctaaagctga cagaacaaaa agacgaacag ctgagccaag ctgtcatcaa
    84841 cgaagctgag caagcacata ttcgatttgt tccgactgtc atcttgggta aagaaatctt
    84901 tgatgaatct atttcaatag aggaattaaa agaattgatc caaacaaaat aaaaaaataa
    84961 ggagcgtagg acaaaagtaa agaacacttt tgtctcacgc tctaaacacc tataaacggc
    85021 ggaagcagaa gcaactcctt cttatttctt ggagttaaac gcttctgtcc cagcctcctt
    85081 attccgaata aatggtgtcc aaaagcttgc atctgccaga ttatctcatt tcttatgaca
    85141 agctgagcaa attatctcca ccaaataatt tattcatatt tttgacttgc gcatactaac
    85201 taattcttaa attttcatac aaaatttctc aaattccctt aacgagtaat atttatgtac
    85261 ggtgattcac actagtatcc caatcacttt ttaagttatc ccgatttcaa ttctacttca
    85321 aaaatagttg cttttatgta tgctgttagt tgattagatg gtcgtttgtt gagctaggtc
    85381 gtttatttta aacaagttgt ttctttagct tatacctatc tcgtctacca aacgcaagat
    85441 ggcaaacaca aatgcatacg catctgtatt tgccactttg attcgtataa agctaacaac
    85501 tcctaactcc tctcatttaa tagttgctct cttttttatt cagatacatt tttgctgatc
    85561 acagcctagt atttcaatgg ttttgaacac taaaccaaat tgttccatta caacattcac
    85621 tggctgatga aatggattaa tagatttcga ttattcaaag cgattttcta ttggtggatt
    85681 gtgttcccaa ctaacaagac ggattccaaa gtcattatat aactcttcaa gagttaaccc
    85741 tttcctgttc ccaatagatg tatctctccc acgctcgata taaaatatgg agtttcgaat
    85801 gccaagacct ggcaaatctt tccttgttac agttacactg ctactaactt ccccatataa
    85861 aatccagctc ctccaaaatg ataatatatt tttcgaccat catctaactc atgtaacaat
    85921 aattgttgtg ccctcttgtc actcgccaaa tgcaaacgcc acgtgcgata ccatgccatg
    85981 ctacctgcat ctgcagaaaa tccatcaaat aatgcaacaa ttgtatcttc tatcacaatc
    86041 ctttccccat ctacttcata ttctaatcta aaatcaaact cccctcgggt aatcgcaggt
    86101 cttggtggct taggacccaa tattattcta cttaaaaccg cattcgttcc cagatgaaac
    86161 acagctataa caacaataat gattaatatt ttgtttctat ttgataaatt tctcattatt
    86221 cgcaaatcac ctctacattc tcacatacta gttcgtaata ctcctttaat atcaaaatat
    86281 ctactgatcg taataatatt cgctatgaat tcctactact cgtataagaa aggccaaatc
    86341 atcagctttt acttgctgat gatttggctt gtttctattt atcgtaattg ttttctaatc
    86401 accaaaattg ttaacaatcg gttcagatat ttcccaattc actagcctta tattatattg
    86461 atttaacaat tcttcttcat ttattgtatt ttgatcaagc ggaccaaatt gttctaataa
    86521 ttctttgtca tgtaaaatat cccttctagt aatgcctctt tccctttcaa tgaaagaagc
    86581 actaggaaaa gtatgattat aaccattcaa cctatctccc atgtaataat tagcaggtcc
    86641 tggaaaataa aatatttgct tattatcact aacttctaac agcaacacta aatcttctcc
    86701 gctggctaaa cgacttgtcc atctacgata tctccctatg ccttcgttca ttccaaatcc
    86761 atcaaattca actattaacg tgtcttctat cacatgtaat tcttcattca attcatactc
    86821 aagtctaaaa ggaaattctc catatgtaat ctctggcaat ggtggtgctg gagttctcca
    86881 aataaataca ataactgtga atagaaaaaa tccaacgatc gatataaatg ttatgagacc
    86941 tgctcgaagt aaaacccgat ataataaaaa tgctgtattt cgctttgcta tatctagaaa
    87001 aataaaaagt actgcaatca taatgatagt aaaagttgta aaaaaacgca tttttgccct
    87061 ccctttataa acatcgattc tcaaattcta aaccaaatct tggtagttca agttcaggta
    87121 taacgtattc caaccataca tctcaattac tatttatgaa cttatgatta tcactgaata
    87181 aatcactcca tccccttgtt aaaacttcta cattaattac acctggttgt tttcgactag
    87241 ctaatccaat gaacttatca accatcctta tccaccaacc acgagaatct tgaccagaag
    87301 taagaagagc agttctcaag gatgatcgag cagttccttc cagtttttta attaatcagg
    87361 ccagttatag ataaatactt gatcttatct atatcaggac ttctatatta agcaaaaatt
    87421 tatgtacaaa actaaaatct tcattcagtt gtctccccgc tttttatacg attgtattaa
    87481 aaacaataga agcctttcca aattatgtta ctccagtaaa gatggaatta tagtttgacg
    87541 gaagaaaagc aagtataact tttaaaaaaa actgatacat tcgcatctcc tgcttcatgt
    87601 atcagttttt tctttggatt aaactgcttc ttcacgcagt ttttccgcaa attcatttat
    87661 tttgcgaatt cggtgattaa ttcccgattt tgatatcgca cctgagggaa tcatttcccc
    87721 taattctttc aagctgacct ctggatgttg aagccgaagt tctgcgattt cctgaagttt
    87781 ttcaggaagg gcatgtagac cgacctttgc ttcgatcaat tcgatgtttt cgatttgttt
    87841 agaagccgca tcaatcgtct tgttcagatt tgctgtctca caattgacta gacgattgac
    87901 cgaatttctc atatcccgta cgattcgaac atcttcaaat cggagcattg agttggttgc
    87961 accaatcaat gttaagaaat cagcaatttt ttctgctcct tttaaataag aaatatagcc
    88021 attgcgccgt tcaagcgttc gagcatgtag accgtaataa tttaacatat cgcaaatgtc
    88081 ttggttatgc tgttcataaa tagaaaatat ttccaaatga taacggctcg tttccggatt
    88141 attgactgaa cctgaggcca taaaagcacc acgtaagtaa gaacgcattt tttgcgaatt
    88201 tcccataatc tcgtccgaaa cgtgtccatt gaacatcata ccatccatga tatcaagatc
    88261 catcaacaca tcttttgtgc cttgcttcaa ccggacgata tagacattgt tcttttttaa
    88321 tttcattttt ttacgaacga gtagctcact tcgtacctga taatgatctt tcaaaagaga
    88381 atagatacgt ctggcaattg cagcattttc tgtttggacg ttcaaaataa actgctggtt
    88441 cacaatacct aaagagccgt tcattcgcag caaagcagct aattccgctt tcgcatgttc
    88501 tcgatgaact tccaaaccag ttagttcttt cttcacatct gcagcaaatg acatcctatt
    88561 ctcctccttt ctccaacaat aaattgctta atattttgaa ccgaaaacta aacggaacaa
    88621 ttcttctact actttgtctc catcgtggaa gacaccacca tctttcaatt ccaaaaagtc
    88681 ggtggaaatc actcgacatc cttcatttct caatccgttg aagtcgtgtg cgacttgtac
    88741 tagatactca tcatagatct ctgggtccat ataatcttca ggcacttttt ccgtattgac
    88801 caatactgta tcgataaaag gctgattcaa atgcttgtgt aaaacacgta tatgatcggc
    88861 atctgtgaaa tgttccgttt cccctttttg ggtcatgata ttacagatat agacggtttc
    88921 agcagttgtc ttgagcattg cttcgccgat ttctggtatc actaaatttg gaaggatact
    88981 ggtgaataaa cttccgggtc ccaaaacgac catgtcggcg tccataattg ctgaaacgac
    89041 ttttcgagcc gctttggctt cacttttatc atttgtgttg cagacataca cacggtcaat
    89101 ggttttacgg tcgatcgcga ttttagattc tcctactgca gtcgttccgt ctttaaagac
    89161 agcatgcaag gtcaaaggag tttctgaaga aggataaaca tgtccatcta catgcatcat
    89221 ttttgctaat aattgaatcg cctcataagt gcttccccgc atctcggaaa ctgcggcaat
    89281 gatcagatta ccgatcgtat gattggctaa aaactgatct tcctttttga aacggtattg
    89341 gaaaatatct tcatagaatt gtggcatatc cgataacgcc actaagacat ttctcaagtc
    89401 accaggagga gccatagcga tcgattctct gattgcaccg ctgcttcccc catcatctgc
    89461 aacagtcact actgctgtga tatctgctcc ttgatttcgc aaacttttca ggataactgg
    89521 cagaccagtg ccaccgccga ccactacgat ctttggtttt cttatccggt aggttttcat
    89581 tttcacgacc ggttcaccgt ctctttcctt ttatccttgt cccgatgtgt cacattgact
    89641 ttatagtttt tccctaaagc atttgccaaa cgttcagtca atgccacaga acggtgttga
    89701 ccgcctgtac agccgattgc aactgtcaag ctgctttttc cttctttaat gtatccaggc
    89761 aatacagttt ccagaagatc caaaaattta tggtagaatg attgcgtttc atcaaaggac
    89821 atcacataat catagactgg ctgatctttt ccggtcaatg gtcttagttc cgggatataa
    89881 tgagggttgg gcaaaaaacg cacatccatc acaatatccg aatcgatcgg caacccgtat
    89941 ttaaaaccaa atgatacaaa ttcgatccga aatccttggt ctccctttga tttgaatgac
    90001 tcattgattt tttcacgtaa ttgtcgtggt gtcaatgtcg tcgtatcaat gatcacggaa
    90061 gcttgggctt tcaagtcgtc tagtatcgcc cgttctttac gtatcccttc agtgatcaaa
    90121 ccgtccatcg ccattggatg tgctcgtctt gtttctttgt agcgagccac tagttcttcg
    90181 tctgaagcat ctaaaaataa gatagaagtg tcgatgaagt tcgtattttc gatttcgatc
    90241 agcatgtttt ggatttcttc aaagaaggaa cgagaacgta aatccactcc taaagccatt
    90301 tttgtgactt tgcctgattc tttgattaat tcccagaatt ttggaatcaa tcgaggcggc
    90361 atattatcga tacaaaaata gcccatatct tcaaaacttt ggatagctac tgttttccct
    90421 gcaccgctca tacctgttat gatgactaat tgcaaattat ctgtcataat tacgaactcc
    90481 tctcacattg tgcatagtaa aattatatca ttccgggcgt atcattacca gttttctttt
    90541 tcttttacaa aaagatttgg accaaatatc ttttaggata tttgccccaa atccaatttt
    90601 tttacttttc cgatgctgta tccaatgcct tgcttctttc agctagcgct cgttcaatcg
    90661 tccgatctgc taaaatagat tgggcatcga tcactggata atggttatct tcagcaaatt
    90721 catgcatcaa tgacaattct gtgcaaccta agataacttt ttcacagttt aggcgctcta
    90781 ccgcttcttc taagatttcg taatatagct cttgattcaa ataatccgat tccttgatct
    90841 catgatagat cagataattg attttttctt gaagaacagt atcaggaatc actgtttcga
    90901 atccaagatt tttgacttcg cgttcataga ttccagcttt catacttcct tctgttccta
    90961 gaatcgctac tctccctgtc gtatgctgac ggacaagttc atttgctgct tctcttggca
    91021 tatgaaggat aggaatatcc gttgccgctt gtaattcttc aaaaaagtag tgtgcggtgt
    91081 tacaagtcaa cacaatgaag ttcggctgca ataaattttg tttctcaata tcatccagca
    91141 gaaacggcat tggattttct tctgatcgat ctaaaatata agctgtacga tcaggaactg
    91201 ttgcatgatt gaataagaca taattcaaat attcttgatc ttttgtagct ttcgttcggt
    91261 ggttgatcaa gcgaacaaaa ctttctgtcg ccatcgtgcc cattccgcct aaaatactga
    91321 aaaaattctc catgaactag ccttctttca catgaaaata ctttttgaat cttggaatat
    91381 agttatggaa catatatttc atcaacaacc agcgcttgat tgaccggtct ttttcataaa
    91441 acacagtagt accgtaacga ttttctttga tcatttgctc agctagtttt ttgtcttcat
    91501 tttcccgagc atattctaaa aagatttttt tgggcacacc catccataat cttgctttat
    91561 ctgattgatt cgttccatag gtcgtttcaa caaagggttt gttaaacact cgatcttctg
    91621 tgacgaaacg agccagattc aagccgttca atgtcacaaa gaaactgcta cgaccttgtc
    91681 tcaaattgat ttcaaataat ttatattctc catctctagg atcatacttc atatcgaaat
    91741 tggcaaagcc tgtatattcg attttttcca aaaatgcctt aatggtttga tagatttttt
    91801 cgttataatc tggcatgatc acaacgtaat taccaatcga tgctggtgta ggatcttcta
    91861 acagtggatg accaagacac atcatccgaa cttgatgatc ttcatctaca tacgcattta
    91921 gcacacgcat attactatca tcacccggga taaaatcttg tacaatcatt tcacttttat
    91981 aaccggcttc gtatatccgg cctaaaatta aatcgaactc ttctctagtc tctaaaataa
    92041 aagctttttt tcttccttca aattggacag aaagatactc cacactgttt gcaggtttca
    92101 aagcaactgg gaattcaaaa ggcaattcct gttccaaatg tccatttact aacatttctt
    92161 ctctgacgat caatgtttta ggatacggaa gatcatattc ttcacaaact tcataaaagc
    92221 ttactttatt gatcagtctt tcaaataaag aataatcaat atatggacag ataaaatatt
    92281 ctgacagctc ttgtttatgt tgggagatca attctgcata tccatcacca caagcaatca
    92341 aaagatattt tttactctta tcagaatatc tttcttttgc caaacgaagc atcgtctcga
    92401 taaacacagg gtctttatca aatcctggaa taacttctac attgacaatt ttactgtagc
    92461 gggtaggggc taactgatcc gaagcatatg cttggcagat tttgccgtat gcttcattaa
    92521 aagaacgtgc cataccataa acgttcatgt cgcttcctaa taatataggg ataaattctt
    92581 cattttcaat actgttcatc attatctctc acttactcat aatctcttgt ttttcttttc
    92641 caactacaca aggaatatca taccataaag aaaaaagcaa tcaaaggaaa ctcctccgat
    92701 tgctcaattc ttttttgaac ggattgaact tataggaagt ttttgtctat accaatcagt
    92761 gtagaaagaa ctaccatgta atgattctag aacactgata ttaataaaac aagcaaaaat
    92821 tcattttttt atgttttttt aactatctca gctatctatc ctctttatac cattattttt
    92881 ttcaaatagt gacctgtata actttctttt acgttaatga tttcttctgg tgttccagtc
    92941 gcaaggatcg ttcctccacc ttctccacct tctggtccta aatcaatgat ataatctgct
    93001 gttttgatta catctagatt gtgttcaatc actaaaactg tgttaccagc atctactaat
    93061 ctttgtaata catgcaacaa gcgggcgatg tcatcgctat gaagtcctgt cgttggttca
    93121 tctagtatat agaaattctt tccattagag attttgtgca attcactggc aagtttcatc
    93181 cgttgtgcct caccaccgga caatgtcgtt gctggttgcc ccatcgtcac ataacctaag
    93241 ccaacatcaa caatcgtttg cagtttgcga tgaatctttg gaatgtgctt gaagaattct
    93301 acagcatctt ctactgtcat ttccaaaata tcagcaatgc tttttccttt gtaatgcact
    93361 tctaatgttt cagagttata tcgtttgcca tgacatactt cacaaggaac atagacatca
    93421 ggcaaaaagt gcatttcgat cttaataatt ccatccccgc gacaagcttc acaacgaccg
    93481 ccttttacgt tgaaactaaa acgccctttc ttataacccc gcatttttgc ctcgttcgtt
    93541 tgagcaaata acccacggat atcatcaaat acacttgtat aagtcgctgg attacttctc
    93601 ggcgtccggc cgattgggct ttgatcgata tcgatgatct tttcgatact ttcgtagccg
    93661 gaaattgtct tgaatttacc tggcttagca gaattcttat ttaatttttg cgctaacgat
    93721 ttctttaaga tactattgac caatgtactc tttccagaac cagacacacc tgtgactgct
    93781 acaaattctc ctaatggaaa ttcaacacta atatttttca gattgttttc tgaagcacca
    93841 gtgatcttga ttgctttccc attaccttta cggcgttctt ttggtacagg gattgatttt
    93901 ttccctgaaa gatatttccc tgtcaaagaa ttttcatcat tagctacttc ttcaggagtg
    93961 cctgcagcca cgatttgtcc gccttgttct ccggccccag gtccgacgtc aatcagataa
    94021 tctgctgccc acatcgtatc ttcatcatgt tcgactacaa cgagggtgtt gccaagatca
    94081 cgcattttct ttaatgagcc aatcagtcga tcgttgtcgc gttgatgcaa tccgatcgat
    94141 ggttcatcta aaatatacaa aactccagaa agattcgatc caatttgtgt agccaatctg
    94201 attcgctgcg cttctccccc agataatgtt ccggaagcgc gactcaaagt tagatagtct
    94261 agtcccacat tttccaagaa tgaaagtctg tcatttactt cttttaaaat cggtttggca
    94321 attactttct cttgttcaga taacgttaag tttgagaaga aatctactgc tttttgaata
    94381 gacaactcat ttgtttgccc gatattcgtt ccattgattt gaacagatag agcttgggga
    94441 ttcaatcgat aacctttaca tgtctgacaa gttaattctg tcatataaag acgcatttgc
    94501 tcacgcgtga aatcactgtt cgtatcatga taacgacgct cgatattctt caatacccct
    94561 tcgaaagtcg tttctacatc gcgaacgcca ccaaaatcat tttcataatg gaaatggaaa
    94621 agatcatctg tcccattcaa gatgatatcc tgatgctctt ccggtaattc ttcgaatggt
    94681 gtatctaaat cgataccgaa actttcacaa gcctgcgcta acatttgagg ataatactga
    94741 gagctgattg gattccatgg aacgatagca ccttgactaa gtgtcttcgt tctatctgga
    94801 atcactaaat cttgatcgac ttctaatttg acacctaaac catcacagtc tggacaagct
    94861 ccaaaaggcg cattgaatga gaacagtctt ggttctagtt cgccaacagt aaatccgcaa
    94921 taaggacagg catagtgttc gctaaacaac atttcttctt caccgatcac gtcaaccaga
    94981 gcatagccgt ctgccaatcg cagagccgcc tctaaagaat caaatagacg agaacgaatc
    95041 ccttctttca caatgatccg gtcgatgacg atcgcgatat catgtttttt gtttttctcc
    95101 agctcaggca cttcacttaa atcatagatc tcaccatcga ctcgaacgcg gacgtatccc
    95161 tctttttgga ttcgttcgaa tattttttta tgttgtcctt ttttctttac cacgatcggt
    95221 gccaacactt ggatctttgt gcgctcaggc aaagccatta cttgatctgc catttgatct
    95281 actgattgac tggtaatctc gatatgatcg ttgggacaaa tcggatgtcc tacacgtgca
    95341 aacaacaagc gtaaatagtc attgatttcc gtcacagtac ctactgtcga ccgaggattt
    95401 ttacttgttg ttttctgatc aatagaaatc gccggactca atccgtcgat gctatctacg
    95461 tccggcttat ccatttgtcc taaaaactga cgagcataag cagaaaggct ctctacgtat
    95521 ctccgctgcc cttcagcgta cagcgtatca aatgctaggg aactttttcc agaaccggac
    95581 aaacctgtaa cgaccacgaa tttatctcgt ggaatcgtta catctatatt tttcaagtta
    95641 tgggcacgtg caccgtgaat cgtaatttta tcgttagcca tgttgtcctc ctactttgaa
    95701 gcttttaatt ctaaaatcat atctcgcaca ttagcggctg tttcaaaatc caatgctttc
    95761 gccgcatttt tcatttcttt ttctaatttc atcaataacg tatctttttc ttctttcgtc
    95821 atttcttcat acgagacttg taccgcttct tttgtatcat cttccgattc tttcgtaata
    95881 gaaatcaaat ctcggatttc tttaatgatc gtttttggaa cgatcccatg ttcttcattg
    95941 tacttctgct gaatcgtacg gcgtcttgat gtttcatcca tcgctagacg catagaatct
    96001 gttactttgt ctgcgtacat gattacttta ccttcttcat tccgagcagc tcgtccaatc
    96061 gtttggacta aggaacgttc actacgtaaa aagccttctt tatccgcatc caagatagca
    96121 acaagggaaa cttcaggtac gtctaatcct tcacgaagca aattgattcc aactaacaca
    96181 tcaaattcac ctaatcgaag atctcgaatg atttctgttc gttccagcgt tttgatatca
    96241 ctatgcaaat atttgacttt gatccctaat tctttgaaat aatctgtgag gtcttctgcc
    96301 atttttttcg tcaatgtcgt tacaaacacc cgctggtctt tttcgactcg ttcatttatt
    96361 tcgcctacta gatcatcgat ttgtcccatg attggacgaa cttcgatcac tgggtcaagc
    96421 agccctgtcg gacggatgat ttgttggata acggtatctg tttgttcatg ttcatatggt
    96481 cctggcgtcg cagagacata aatgatctga ttgacatgtt tttcaaactc ttctaaccga
    96541 agtggccgat tgtctaaagc agatggcaaa cggaaaccgt aatctactag catctgtttt
    96601 ctcgctcggt caccattata cattcctctg acttgaggca tggttacgtg cgattcgtcc
    96661 gccacgatca aaaaatcttc aggaaagaaa tccaatagcg tataaggcgg ttcaccttct
    96721 ttacgtccgt ccatatgccg agaataattt tcaattcccg acgtgtagcc catttctaac
    96781 atcatttcaa tatcgtaatt ggttcgctgt tctaaacgtt gagcttccag aagtttgttt
    96841 tcatttcgca agacagttaa acgctgttct aattccgctt tgatattcgc tacggcatgc
    96901 tccatatgtt catcattcgt cacaaagtga gtcgccggga agattgccac gtgttccgtt
    96961 tctcctaaaa cttcacctgt cagtgcattg acttctcgga tacgttcgac ctcatctcca
    97021 aaaaactcca cacgtaaggc acgttcatct cttgaagccg gaaaaatctc cacgacatca
    97081 ccgcgcactc gaaaacgacc gcgctgaaaa tctatatcat ttctttcgaa ttggatactg
    97141 actaagtcac gaatcaactg attgcgatct agttctgctc cctgtcgaat cgaaactact
    97201 tgtttttggt actcaaaagg cgaacctaat ccaaaaatac atgaaacgga tgcaatgacg
    97261 atcacatcat ttctttctaa taatgaacta gtagccgaat gtcgtaattt gtcgatctca
    97321 tcatttacac ttgaatcttt ttcgatatac gtatcgcttg aaggcacata agcttcaggc
    97381 tgataataat cataataaga aacaaaatat tcaacagcat tgttagggaa aaattccttg
    97441 aattccccgt acagctgacc agccaatgtt ttgttatggg caataattag tgttggttta
    97501 ttcactttct caataagatt ggaaatcgta tatgttttcc ctgttccagt cgcacctaat
    97561 aagatttgtg cttttttccc gccgactacg ccatccacta attggttaat cgcttcaggc
    97621 tgatcaccag ccggttggta tttcgagaca agctcaaagt gtcgggaggt atctctctca
    97681 atcattcttc gtcctccttt tcattcattc cttttcattt tacgaccaat aaaaagaaaa
    97741 ggaaagcaaa ggcattcctt actaaaaaat tataccacac cgaacatact ttcgccactt
    97801 ttttatctta ttatgcatgt tacgttttat aacataaaat gttagaaact cgcatttttt
    97861 tcattttttc gagcttacag tcttgtctga ttcagcactt ttcgcctata ataataaaat
    97921 attggaaatt taggaggttg attatgaaaa gaaagcattt atttcactca tttgttttct
    97981 taatgacagg acttgtgaca cttgcgtttg gacatgttgc acaagctgac aacactacaa
    98041 cggcagagtc aagcacaagc gagacaatgc aaaaaataga gcccaaaaaa gacgtatacg
    98101 taattgccag tgactctgct tttgctccat ttgaatttca aaacgcaaag ggcgagtacg
    98161 aaggcatcga tgtaaaattg atggaaaaca tcgccaaact gcaaggattt aacatccgaa
    98221 tggactaccg tggtttctct gctgctttgc aagctttgga atcaggacaa gcagacggaa
    98281 tgattgccgg aatgactgta acagatgaac gaaagaaaag ctatgatttt tctgattctt
    98341 actttgatag cgggatccaa ttagctgtca aaaaaggaga tacttcgatc aagtcttatg
    98401 aagatttgaa aggaaaaaca gtcggagcaa aagttggtac cgaaagtgct gaatttttaa
    98461 gcgaccatga atctgaatat ggctattcta tcaaatattt tgatgctgct gatcaattgt
    98521 acgacgggtt aaaagttggc gcaatcgatg caatgatgga tgattatccg gtaatcggtt
    98581 atgcgatcaa acaaggacaa gaattggaaa caccgatcaa aaaagaatct ggcggaaaat
    98641 atgcatttgc cgttaaaaaa ggacaaaatc ctgagcttct taatatgttc aatgaaggat
    98701 tgaaagaatt aaaaagaaca ggcgaatacg ataagattat taaagaatat gtagcggatg
    98761 gttcagaaac ttccagtgag tcttccactg ctgatgaatc aacatttctt ggacttatcc
    98821 aaaacaacta tcaagcattg ttgaaaggac tttggagaac aattctccta actttgatct
    98881 cctttgcttt ggctatagta atcggtgtga tttttggatt attcagtgta gcaccaatac
    98941 gcgctcttcg cacaattgct agtatttatg tcgatatcat ccgaggaatt ccaatgatgg
    99001 ttcttgcgtt ctttattttc tttgggttgc ctggtatcgt tggatttacg attcctgact
    99061 tccttgctgg tgttatcacc ttgactctta acgcaagtgc ttatatcgca gagattgttc
    99121 gaggcgggat caacgccgtt cctgtcggac aaatggaagc ttccagaagt ttaggtcttt
    99181 cttataatcg gacaatgcaa aaaatcattt tgccgcaagc gatacgcatc atgatcccat
    99241 cattcgtcaa tcagttcgtt atctcattaa aagacacaac gatcatttcc gcaatcggtg
    99301 tagtcgaatt gctgcaaact ggtaagatca ttgttgcacg tacaacacaa agtacgtatg
    99361 tttacttgat cattgcgatc atgtacttga ttttgatcac agcattgaca aaattagcaa
    99421 aagttttaga aaagaaggtg aagtaagatg gcagaaaaaa tcttagttga gcatttagtc
    99481 aaaaaatacg gcgataacac agtattaaac gacatcaatg tgtccatcaa cgaaggagat
    99541 gtcgtctgta tcattggccc ttcaggttca ggaaaaagta catttttgcg ttgcttgaat
    99601 cgtttagaag aaccttctga cggtgaaatt attattgacg gcgcccactt gatggataaa
    99661 agcacagata tcaacttggt gagacaacat atcggaatgg tattccagca ctttaaccta
    99721 ttccctcatc tttctatttt ggaaaacatc attttagcac cgatggacct gaagaaagaa
    99781 tctagagaag aagcagagaa aaaagcaatc aaattgctgc agacagttgg tctagaagat
    99841 aaaaaagata tgtatcctga aatgctttcc ggtggacaaa agcagcgtgt ggctattgct
    99901 cgtgctctag cgatgaaccc tgacatcatg ctttttgatg agcctacatc tgcactcgac
    99961 cccgaaatgg tcggtgatgt cttgaacgtc atgaaaaagt tagcagaaca aggtatgacg
   100021 atggttatcg ttacgcacga aatgggcttc gctaaagaag tggccaatcg tgtcatgttc
   100081 attgacgacg gaaacttttt agaagacgga acgcctcaac aaatctttga aaatccgcaa
   100141 aatgaacgga caaaagattt cttgaataaa gtattaaata tttaataaaa accaaaaatg
   100201 cccggtcgct tcatgacgac cgggcatttt tagtttcttg ttttttcact taactgtacc
   100261 agccgcatcg cttgttctcc tgcacaaaca agcaatcgct ctacctgttc catcgcataa
   100321 gaaagtacca tcggttcatt gacgatatca agtacatagt cgataccacc ttcagaaact
   100381 atgatgatta tcttattcta aagctggctt ttttgttggt acaaacatga caactacaaa
   100441 aaaa
//
LOCUS       DAZBSA010000002        59371 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000002 DAZBSA010000000
VERSION     DAZBSA010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.002"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..417
                     /locus_tag="ACHGMT_000096"
     CDS             <1..417
                     /locus_tag="ACHGMT_000096"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFN6833877.1"
                     /translation="TPYIIEDKEMSPYYAQGNLIYVRPKNTNEILVGDIITYYENSGK
                     KISTRRVIAVENNHEDFYTKADTKTYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFA
                     WIQGLFFTIAFCLTGITAWNTFVELKKKRRRTQKFL"
     gene            545..1228
                     /locus_tag="ACHGMT_000097"
     CDS             545..1228
                     /locus_tag="ACHGMT_000097"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFN6833878.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1537..1610
                     /locus_tag="ACHGMT_000098"
     tRNA            1537..1610
                     /locus_tag="ACHGMT_000098"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1571..1573,aa:Arg,seq:tct)
     gene            1769..2128
                     /locus_tag="ACHGMT_000099"
     CDS             1769..2128
                     /locus_tag="ACHGMT_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFN6833879.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2251..3882
                     /locus_tag="ACHGMT_000100"
     CDS             2251..3882
                     /locus_tag="ACHGMT_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFN6833880.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQFPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(3944..4585)
                     /locus_tag="ACHGMT_000101"
     CDS             complement(3944..4585)
                     /locus_tag="ACHGMT_000101"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFN6833881.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4771..4847
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            4867..5175
                     /gene="rplU"
                     /locus_tag="ACHGMT_000102"
     CDS             4867..5175
                     /gene="rplU"
                     /locus_tag="ACHGMT_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFN6833882.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5202..5546
                     /locus_tag="ACHGMT_000103"
     CDS             5202..5546
                     /locus_tag="ACHGMT_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFN6833883.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5559..5852
                     /gene="rpmA"
                     /locus_tag="ACHGMT_000104"
     CDS             5559..5852
                     /gene="rpmA"
                     /locus_tag="ACHGMT_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFN6833884.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6286..9093
                     /locus_tag="ACHGMT_000105"
     CDS             6286..9093
                     /locus_tag="ACHGMT_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFN6833885.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9284..9775
                     /locus_tag="ACHGMT_000106"
     CDS             9284..9775
                     /locus_tag="ACHGMT_000106"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFN6833886.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            9878..10867
                     /locus_tag="ACHGMT_000107"
     CDS             9878..10867
                     /locus_tag="ACHGMT_000107"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFN6833887.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            10900..11703
                     /locus_tag="ACHGMT_000108"
     CDS             10900..11703
                     /locus_tag="ACHGMT_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFN6833888.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11724..12638
                     /locus_tag="ACHGMT_000109"
     CDS             11724..12638
                     /locus_tag="ACHGMT_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN6833889.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12721..13095
                     /locus_tag="ACHGMT_000110"
     CDS             12721..13095
                     /locus_tag="ACHGMT_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFN6833890.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13234..13479
                     /locus_tag="ACHGMT_000111"
     CDS             13234..13479
                     /locus_tag="ACHGMT_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFN6833891.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13651..14715
                     /locus_tag="ACHGMT_000112"
     CDS             13651..14715
                     /locus_tag="ACHGMT_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFN6833892.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            14961..15386
                     /locus_tag="ACHGMT_000113"
     CDS             14961..15386
                     /locus_tag="ACHGMT_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFN6833893.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15379..15828
                     /gene="nusB"
                     /locus_tag="ACHGMT_000114"
     CDS             15379..15828
                     /gene="nusB"
                     /locus_tag="ACHGMT_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFN6833894.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            15925..16779
                     /locus_tag="ACHGMT_000115"
     CDS             15925..16779
                     /locus_tag="ACHGMT_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFN6833895.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            16780..18117
                     /gene="xseA"
                     /locus_tag="ACHGMT_000116"
     CDS             16780..18117
                     /gene="xseA"
                     /locus_tag="ACHGMT_000116"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFN6833896.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18122..18346
                     /locus_tag="ACHGMT_000117"
     CDS             18122..18346
                     /locus_tag="ACHGMT_000117"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFN6833897.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18348..19238
                     /locus_tag="ACHGMT_000118"
     CDS             18348..19238
                     /locus_tag="ACHGMT_000118"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFN6833898.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19247..20065
                     /locus_tag="ACHGMT_000119"
     CDS             19247..20065
                     /locus_tag="ACHGMT_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFN6833899.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20172..20621
                     /locus_tag="ACHGMT_000120"
     CDS             20172..20621
                     /locus_tag="ACHGMT_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFN6833900.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20639..22321
                     /gene="recN"
                     /locus_tag="ACHGMT_000121"
     CDS             20639..22321
                     /gene="recN"
                     /locus_tag="ACHGMT_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFN6833901.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22376..22492)
                     /locus_tag="ACHGMT_000122"
     CDS             complement(22376..22492)
                     /locus_tag="ACHGMT_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFN6833902.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22565..23521)
                     /locus_tag="ACHGMT_000123"
     CDS             complement(22565..23521)
                     /locus_tag="ACHGMT_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFN6833903.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKPFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23692..24060
                     /locus_tag="ACHGMT_000124"
     CDS             23692..24060
                     /locus_tag="ACHGMT_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFN6833904.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24230..24661
                     /gene="mraZ"
                     /locus_tag="ACHGMT_000125"
     CDS             24230..24661
                     /gene="mraZ"
                     /locus_tag="ACHGMT_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFN6833905.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24682..25641
                     /gene="rsmH"
                     /locus_tag="ACHGMT_000126"
     CDS             24682..25641
                     /gene="rsmH"
                     /locus_tag="ACHGMT_000126"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFN6833906.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25646..26047
                     /gene="ftsL"
                     /locus_tag="ACHGMT_000127"
     CDS             25646..26047
                     /gene="ftsL"
                     /locus_tag="ACHGMT_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFN6833907.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26048..28240
                     /locus_tag="ACHGMT_000128"
     CDS             26048..28240
                     /locus_tag="ACHGMT_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFN6833908.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28266..29228
                     /gene="mraY"
                     /locus_tag="ACHGMT_000129"
     CDS             28266..29228
                     /gene="mraY"
                     /locus_tag="ACHGMT_000129"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFN6833909.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29242..30603
                     /gene="murD"
                     /locus_tag="ACHGMT_000130"
     CDS             29242..30603
                     /gene="murD"
                     /locus_tag="ACHGMT_000130"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFN6833910.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30629..31717
                     /gene="murG"
                     /locus_tag="ACHGMT_000131"
     CDS             30629..31717
                     /gene="murG"
                     /locus_tag="ACHGMT_000131"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFN6833911.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31732..32952
                     /locus_tag="ACHGMT_000132"
     CDS             31732..32952
                     /locus_tag="ACHGMT_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFN6833912.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33108..34433
                     /gene="ftsA"
                     /locus_tag="ACHGMT_000133"
     CDS             33108..34433
                     /gene="ftsA"
                     /locus_tag="ACHGMT_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFN6833913.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34456..35697
                     /gene="ftsZ"
                     /locus_tag="ACHGMT_000134"
     CDS             34456..35697
                     /gene="ftsZ"
                     /locus_tag="ACHGMT_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFN6833914.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35714..36391
                     /locus_tag="ACHGMT_000135"
     CDS             35714..36391
                     /locus_tag="ACHGMT_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFN6833915.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36405..37004
                     /locus_tag="ACHGMT_000136"
     CDS             36405..37004
                     /locus_tag="ACHGMT_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFN6833916.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37085..37306
                     /locus_tag="ACHGMT_000137"
     CDS             37085..37306
                     /locus_tag="ACHGMT_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFN6833917.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37355..38137
                     /locus_tag="ACHGMT_000138"
     CDS             37355..38137
                     /locus_tag="ACHGMT_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFN6833918.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38158..38865
                     /locus_tag="ACHGMT_000139"
     CDS             38158..38865
                     /locus_tag="ACHGMT_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFN6833919.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39127..41925
                     /gene="ileS"
                     /locus_tag="ACHGMT_000140"
     CDS             39127..41925
                     /gene="ileS"
                     /locus_tag="ACHGMT_000140"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFN6833920.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(41974..43500)
                     /gene="zwf"
                     /locus_tag="ACHGMT_000141"
     CDS             complement(41974..43500)
                     /gene="zwf"
                     /locus_tag="ACHGMT_000141"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFN6833921.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43515..44162)
                     /locus_tag="ACHGMT_000142"
     CDS             complement(43515..44162)
                     /locus_tag="ACHGMT_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFN6833922.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44346..44675)
                     /locus_tag="ACHGMT_000143"
     CDS             complement(44346..44675)
                     /locus_tag="ACHGMT_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFN6833923.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            44852..45580
                     /gene="sfsA"
                     /locus_tag="ACHGMT_000144"
     CDS             44852..45580
                     /gene="sfsA"
                     /locus_tag="ACHGMT_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFN6833924.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45596..46609
                     /locus_tag="ACHGMT_000145"
     CDS             45596..46609
                     /locus_tag="ACHGMT_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN6833925.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46609..47886
                     /locus_tag="ACHGMT_000146"
     CDS             46609..47886
                     /locus_tag="ACHGMT_000146"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFN6833926.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            47949..50651
                     /locus_tag="ACHGMT_000147"
     CDS             47949..50651
                     /locus_tag="ACHGMT_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFN6833927.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            50803..51120
                     /locus_tag="ACHGMT_000148"
     CDS             50803..51120
                     /locus_tag="ACHGMT_000148"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN6833928.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51150..51470
                     /locus_tag="ACHGMT_000149"
     CDS             51150..51470
                     /locus_tag="ACHGMT_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFN6833929.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51578..53038
                     /locus_tag="ACHGMT_000150"
     CDS             51578..53038
                     /locus_tag="ACHGMT_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFN6833930.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53106..53327
                     /locus_tag="ACHGMT_000151"
     CDS             53106..53327
                     /locus_tag="ACHGMT_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6833931.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53358..53540
                     /locus_tag="ACHGMT_000152"
     CDS             53358..53540
                     /locus_tag="ACHGMT_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFN6833932.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53540..53953
                     /locus_tag="ACHGMT_000153"
     CDS             53540..53953
                     /locus_tag="ACHGMT_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFN6833933.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54076..55257
                     /locus_tag="ACHGMT_000154"
     CDS             54076..55257
                     /locus_tag="ACHGMT_000154"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6833934.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55328..55492)
                     /locus_tag="ACHGMT_000155"
     CDS             complement(55328..55492)
                     /locus_tag="ACHGMT_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6833935.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55646..55719
                     /locus_tag="ACHGMT_000156"
     tRNA            55646..55719
                     /locus_tag="ACHGMT_000156"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55680..55682,aa:Arg,seq:cct)
     gene            complement(55788..56927)
                     /locus_tag="ACHGMT_000157"
     CDS             complement(55788..56927)
                     /locus_tag="ACHGMT_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6833936.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            57099..57233
                     /locus_tag="ACHGMT_000158"
     CDS             57099..57233
                     /locus_tag="ACHGMT_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6833937.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            complement(57226..57861)
                     /locus_tag="ACHGMT_000159"
     CDS             complement(57226..57861)
                     /locus_tag="ACHGMT_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6833938.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(57974..58609)
                     /locus_tag="ACHGMT_000160"
     CDS             complement(57974..58609)
                     /locus_tag="ACHGMT_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFN6833939.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(58643..59104)
                     /locus_tag="ACHGMT_000161"
     CDS             complement(58643..59104)
                     /locus_tag="ACHGMT_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFN6833940.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(59234..>59371)
                     /locus_tag="ACHGMT_000162"
     CDS             complement(59234..>59371)
                     /locus_tag="ACHGMT_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HFN6833941.1"
                     /translation="LTDESQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQY
                     V"
BASE COUNT        19463 a        10026 c        12566 g        17316 t
ORIGIN      
        1 actccttata ttattgagga caaagaaatg agtccatatt atgcacaagg aaatctgata
       61 tatgtacgac cgaaaaatac aaatgagatt ttagtcggag atattattac atattatgaa
      121 aattcaggaa aaaaaatctc gacgcgtcgc gtaatagccg ttgaaaacaa ccacgaagat
      181 ttttatacaa aagctgatac aaagacttat attgagcctg gagtagtaaa aaagagaaat
      241 attatcggta gtcccatctt tcagattcca tatattggac tagtcctttc aaaaacggca
      301 tttgcatgga ttcaggggtt attctttacg attgcttttt gcctaacagg aatcacagca
      361 tggaatacct ttgtagagtt gaaaaagaag cggagacgaa cacagaaatt tttgtaagaa
      421 tgttaaaaac ataaatgtat tccagagatc caacgactct tctgaacatg aaacacgaga
      481 aaacactcat gcagcttgta gatttagtca gttgttcgta taatgtgtga agaggaggag
      541 tagtatgcgc aatgaaatga tgcatcgtcc tgtagtcaaa gaagaactat tggattttat
      601 gcgtacccag caaaaaaaat taccgggtga gctaggcaaa ctggaagaag aagctcatgt
      661 agcagaagta ccagttattc cccatgaaac agttgtattt ttgaaatttt tacttggaca
      721 gcttcagcct gaacgaatct tggagatcgg agcagctatc ggtttttcat caagtgtcat
      781 ggccacgaca atcggagaaa acgctcatgt tacgacaatt gatcgtttcg atgtcatgat
      841 cgagaaagct aaaaaaaact atgaacgtct aggactgaca gataaagtga ctttgcttga
      901 aggtcaagca gcagatatct tgccggagtt gtctggtccg tatgacttta tttttatgga
      961 tagtgcaaaa tcaaaatata ttgaattttt acctgaatgc ttacgtttgt taagaaaagg
     1021 tggcgttttg atggtagatg acatcttcca aggaggaacg atcttgctcc cagatgaaga
     1081 gattccccgt ggcaaacgag ctatccatcg taaattaaat gaatttcttc gtgttgtgat
     1141 ggatcatccc gatttgacat cgacaatctt gccattaggt gatggtgtga tcttaatgac
     1201 aaaggaatca gaaacaatcg agttgtgata catgattatg gcagcctgta gataatcgat
     1261 tgaatcgagg gctcaattcg aacttgatgt aaaagtttct atatttgtaa gtcaattaaa
     1321 ggatacagga aatctaaata aacaggagta gcatgaagtc aatgtagctc ctgttttcaa
     1381 ttgataacct tagaacagct gaaggagaat gtctcgtttt aaaagattga tttagtagtt
     1441 gggccaaaat aattcattat ttgctaatta aataactatt tcgtattgac agaagcaatt
     1501 taattatcta taattattga tgttccgctg attcttgtca tagtagctca gctggataga
     1561 gcatccgcct tctaagcgga cggtcggggg ttcgaatccc tcctgtgacg ctaaaaaacc
     1621 gttagaaact gaggtttcta acggtttttc tttgtttttt gtgccaatat tgtttgtttt
     1681 caaaggcaaa taaaataaaa aaacatcctt atcgagtaca attataagga gcaaaaaaat
     1741 aaaactcatc agaaaagagg aagaagatat gacgatacta tctagaattt tagtgactct
     1801 agttgcaatt gaattttttt atatcatgta tatcgaaacg gtgagaactg attctgacac
     1861 gacaagtagg gtttttaaaa tgagtaaaga agagttaagc agaaaatctg ttcaaacatt
     1921 gtttaagaac caaggagtgt acaatggact attaggagtg ggactacttt atggagctta
     1981 tctttcttcc gcatctaaag aaatcacaag tatgctatta atatctattt tttttgttgc
     2041 attatatggc agtttgacta gcgatcgttg gattttactc aaacaaggtg ggctagctct
     2101 gttggcattg ctttctattt tcttataaat atagaaaaaa taagaattta tctaacagga
     2161 aactttgtga aaaatattac tttatctcat gaaatatgat attctaatga atggtatttt
     2221 agaaaatttc atgaaaaagg agttagatag gtgttcaaaa attatgtttc ctcactaaaa
     2281 aaagaatttt caggttatga tctttcaaga tttcagaaag atcttttggc tgggatcaca
     2341 gtagctgctg tagcattgcc tttagccttg gcttttgggg tatcaagcgg agctgatgcg
     2401 gccgcaggtt tgattacggc tatcattgcc ggtatcgtta ttggcggttt atcaggcggt
     2461 ttttatcaaa tatcaggtcc gacaggtgcg atggcagcta tcttgatgtc tattgcggca
     2521 acaaagggaa tgcaaggtgt attgcttgct acttttttag cgggtctatt tttacttgca
     2581 gcaggacttt tccgattagg aacactgact tcttttatcc cagcacccgt aattacgggg
     2641 tttacttcag gtattgctat cattattgca ttaggtcaga tcgataattt atttggtgtt
     2701 cactcggaag gtacaaacgt tattgaaaaa ctaagtagtt atcaaacgat aggatttccg
     2761 ataaacacag cctctttgat catgggaggg ctagtcattt tagggatgtt tatctatcca
     2821 aaaaaatggg cgaaacgaat gcctagttcg ctgctggcta tcattttggc aacagcagtt
     2881 atgctattgt tccatttacc agtagcaact gtgggtaaga ttccacaaac attgatcagt
     2941 agtaatcgtt tgaatatggg agattttagc ttttctgcct tgcaacaagt ggcagttcca
     3001 gctattagca ttgctttatt aggaatgatt gaaagcttgc tatgtggtgc ttcggctggc
     3061 cgaatggcaa ataagccctt agatagcaat caagaattaa tggcgcaagg aattggaaat
     3121 ctgttgcttc ctttctttgg cgggattcct gctactgcgg ccatcgcccg tacaagcgtg
     3181 gccatcaaat ctggtgcaca aactagatta gctggtatga tacatgcagt tgtcttactg
     3241 ctctccatgc ttgtttttgc gccagtgatg tcacagtttc caatgccagc acttgcaggt
     3301 gttttgattg tgacggcttg gcgtatgaat gaatgggaaa cgatcaagga gctatttact
     3361 aataaatatt ggtcagcagt tctattgttt atccttacga tgatttgtac agttatcttt
     3421 gatttaagta ttgctatcgt gatcggggtg atcggcggct gtgtattttt tgttgtcaaa
     3481 agcgctgcta tcacgatttc attagaaacg atcgattggc aaagaatggg gctgccagaa
     3541 acggaaaagc tgaataactg gacagtagtg tatattagtg gtccactgtt ttttatgtct
     3601 tcagaaaaac tcaaaaataa attggaagaa ctaaatcatc acgatggtgt cattttctcc
     3661 atgcgaggcg taccaagtat tgatttgacg gcacgtgcag tttttgatga atttcaagaa
     3721 aaagcagagc aaagaggaca aacgattatc tatacttcct tgcagccaga agtagaaaaa
     3781 caactttctc acttgtggga acatacattc acgcatcaac atcaaacagt tgctcatgcg
     3841 ttgacagctt tacatgcgaa atatactgca attagccatt aaaacaatga aaaagaacag
     3901 acaagactgt aaactgaaga ttttatggtc ttgtctgttc tttttaatat tgctgtaaaa
     3961 gtgaagctag tacttgccag tcttgatgga taacaggtac aagggagcga ttgtagaaaa
     4021 tagctgctgg atgaaaaagt ggaagaattc gatagttttt ttctgaccat tcgtaggcat
     4081 ccttttgttc tgttaattgt aagacaggcc ctgtatagag ttgaccatga tttttggtaa
     4141 ccgtatattc tttgccaagt aaacgttgaa gaccaatgtt tccaagtgtc gcaattaatt
     4201 ttggctggac cttcatcagt tcgtaatcca atattggtgc atgcgcaaat acttcggaac
     4261 gagtaggtgt ccgatttgga taaacagttt ctgactgttg tgtccgttta ttgatccgat
     4321 gagtcacgcg atacgggcgg ctccttacag cactggtgat atacacctct tctcgtgtta
     4381 agccagttga agaaagtgcc tgcatcaatt cttttccagc ttgaccgcta aaaggaacat
     4441 gcgtatcaat ctctgttttc cctggggctt ctcccacgag catcagttta ggatgaagag
     4501 gtccttgccc agcgataaat ccttctacag gacggccgtt cattcttaaa gaagcttctt
     4561 gtatcagttc ttttgggtat tccatttatg ctcctccttt ttcttttatt ataagaataa
     4621 aagaaacaca atgcgactat tttatttggt agaactgtga ggatagaaaa gcagaagtat
     4681 tgcttgaatt tttgttttac tttttgattt taaatttagt tgactaacgg ttgctgttct
     4741 ggtaagctag tactgttgta attgtggact ccacagctac aaccgctcag aacaagtaat
     4801 aagcacagca tatgctgtca cttcggatgg cgagtctaag tatatcagaa ggaggtgctt
     4861 tatagcatgt acgcaattat taaaactggt ggtaaacaag taaaagttga agtgggacaa
     4921 gcaatctatg ttgaaaaact tgacgttgaa gcaggagaaa aagttgtttt tgacgaagtt
     4981 atcttagtag gcggagaatc tacaaaagta ggcgctccaa ctgttgcagg tgcaactgtt
     5041 gaaggtactg ttgaaaaaca cggcaaacaa aagaaagtcg ttactttcaa atacaaacct
     5101 aaaaaacatt cacaccgtaa acaaggtcat cgtcaaccat atacaaaagt ggttatcgac
     5161 gcgatcaacg cttaatagcg tttgagaaag aaggcctata tatgatcaaa ggaacattta
     5221 agcgaaatga cgcaggtcag atcgtttcat tcactttgac cgggcatgct gatgctggtc
     5281 cttatggaag tgatatcgtt tgtgcgggtg tttctgcgtt agcgatcagc actgtcaacg
     5341 ggattgcctc attagccggt tttgagccga ttgttgaaat gaatgaagaa gaaggcggtt
     5401 atctttatac cgaagtcact tcaggaatga cacaagaaca aaacaatatt gctgagattc
     5461 tattagaaaa tcttttatta ggattacaat cgatagaagc tgaaaactca gaatatatcc
     5521 aaattaaaac aattaatgat aaataggagg tgcagaccat gttgttatct atgaatctac
     5581 aattatttgc ccacaaaaaa ggtggcggtt ctacatctaa cggccgtgac tcagaatcaa
     5641 aacgtctagg tgctaaacgt gctgatggac aaacagttac aggtggatca attctttacc
     5701 gtcaacgcgg tacaaaaatc tatccaggtg taaacgtggg tatcggtgga gacgacactt
     5761 tatttgcaaa agttgatggc gtagtacgtt tcgaacgtaa aggccgtaac aaaaaacaag
     5821 tgtctgttta tccagtagct caagaagctt aattgaatct tatgattaaa gaggttgtga
     5881 caagactttt gtcacagcct tttttgttta taaaaataaa agttgtagtg gaggctacga
     5941 agtttacttt gtctttttac acttaatatt tttttgccaa tttggaatca ggatgttttg
     6001 ataactatcg ttttcaattg atggaagcta tattgtttgc tatcagtgaa ataattatat
     6061 ggtaaacagt gttctttttc ttgtttccat tataaaaatc gtatcgggat ttttcgttct
     6121 tttaaattga acatttctat ctatccgcgt gttatcaaca aaacaaaatc aggtaaatag
     6181 ttgacgaaac agaaataaat acatagtatt atgttttgaa gcctaagtac aatagtctat
     6241 agaaaaggag cgataccatt ggcaaaacgc attgaccgaa tttatatgta tgtcaaagaa
     6301 aaaaccgctc atttaacaaa ggcagaatat gatcaaggag taacaacaca ggaaattgct
     6361 gaagtgttag ggattcagcg aacaaatagc agtaaagatc tgaatcagct agtgcgcgaa
     6421 ggaaaactga taaagacaga cggacgacca gtacgttaca tatatcaaac actcgttacg
     6481 cacgtaaagc cagttacaaa acatgtagtc agttacaaag aaacgccggt ctctgaagaa
     6541 aaaaatacac tccaaataga tactaaggat atctttgcaa aaatcattgg agctaacggc
     6601 agcatgaaaa attcagtgga acaggcaaaa gctgcgattt tgtatccgcc aagaggatta
     6661 aattgtttga ttacaggacc taccggatca ggaaagactt attttgcaca cgcgatgtat
     6721 cattttgcaa aagcgcatca tgtaatagat gaggaaacgg aactgattgt tttcaattgt
     6781 gctgattatg ccaataaccc tgagcttttg atgagtcatt tatttgggta cgcaaaaggt
     6841 gcttttacag gcgcagatga agaaaaaatg gggattatcg atcaagcgga tggcggtatg
     6901 ctgtttttgg atgagattca ccggttgccg cctgaaggtc aagaaatgat cttttacttt
     6961 atggatcatg gaacttacag tcgtcttggg gaaacaacga aatcccatga agcaaatgta
     7021 cggatcgtag gtgcgaccac agaagaccca ggttcttctc tattagaaac ttttgttcgg
     7081 cgtatcccaa tcaatatcaa acttccggct tttgaaaaac gtcctgcaaa tgagaaagtc
     7141 gatttagtaa aaatcatgat tgcccacgaa gcaaaccgca cgcagcgaaa gatttctttg
     7201 acagaagatg tagcaaaagc attgatcggc agtgtgacct atggaaatat aggacaattg
     7261 aagtcaaata tccagttagt atgtgcacga ggatttctaa atcatatgca atcacccgaa
     7321 atctcgatca caatcgatga tttgacagaa gggattcgta gcggattgat tcaattagca
     7381 agcaaccgca gtgcaatggc agagctttct aaaatattgg agccaaagat aactgtagca
     7441 ccaaatgata cgatcatgaa aatccaatcg gactcctatg agttacctta taatctatat
     7501 gatatcatcg gagataaagc agctttactg aagtcggatg gtttggatca agaagcaatc
     7561 aatcatttca tttctacaga tatcaacatc catctgaagt ctttctataa agaccatggt
     7621 ttttctttta atgcagacag caaattagca gaatttgtag atccgaaaat catagaagtg
     7681 accaatcaga tttatgtaat ggtcaaaaga tctttaccat atgagtttca gcaaaacttt
     7741 atttatgcta tgagcttgca tatcagttct tttttgaaaa aaatcaactt aggagaagaa
     7801 cggcatacga atgataacat ccgtgagatg gcttttgatt ttccagatga atatgctgtt
     7861 gcgaaagaag tgcgtagtta tatcgaaacg tattttcaag tggagattcc agaaagtgaa
     7921 gactattact tgaccgttct actcgtctcg ttacgggcaa atcaagcaag cggcagaatc
     7981 ggtgtagtcg ttgcagctca cggaaacagc acagcaagca gtatggtcca agttgtccaa
     8041 cagttgttag atgcagagaa cgtacgagca gtagacatgc ctttggatat ggatccgaaa
     8101 acagcattgg accgaattga acgcaatgta caagaagtag atgaaggaag cggggtcata
     8161 ctcttagtag atatggggtc ccttgcgtcg tttaattcgc aaattcaaag agacactgga
     8221 attccagtac gtactgttga tatggtaaca acttctctag ttttggaaac tgtacgaaag
     8281 gtctcagtat tagggacaga tctagatatg ttgcatgaat cattgaaaaa ttttcgaggt
     8341 tacgcagaaa tatcttctgt agaatctgtt caggaagcaa aacctattca taagaaaaaa
     8401 gctgttttgg ctatctgcgc atctggcgaa ggtacagcac aaagaatcaa agaattgatc
     8461 gaacgagcgg taaacaagcg tcaagaaaca gaattagaag ttctagcttt gtcgattgtg
     8521 gaagtgaaag aagaactgcc agttatccaa aaagaatatc agatcatagc tactactggg
     8581 attactgatt tgaatatcca agccccattt attcctttag aacgttttat tgatcaaaat
     8641 atcgaagtca ttttggatca gttgctgatg gaatcagaat tggaggaaac tgaattcatc
     8701 tccctagatg aagagtcagc taaaaatacg tgtgttgagt ttatctctga taacttcatc
     8761 tttattaatg gtagtaagtt aatcgatcca atgtggcaat tttctacaca aatctctcag
     8821 acgacgggaa tcggcgatga agaatatgga ttcaaaatca acctagtgat gcacacagca
     8881 ggaatgatcg aacgtattat tcggaatgaa ccattgactg tggaagaaaa cgaattgaca
     8941 aatacaacga atgatcctct ttatagccaa ttggcagcct cagttgttct gttggaagat
     9001 cagataaaag taaaagtgcc gatagaagaa atgtattatc tgctacgtct agtccataat
     9061 cagttagata aaaaagaata cactgtgcca tgaatacaca aaaacgatac acttttatag
     9121 tgtatcgttt ttgtgtattg gttaagaatc ctttaaattc aattagtgta tcgtaaaaaa
     9181 gttggcacgc tttttgctta tagtagtgtg tgctaggagg tggttgttca cacagaaatg
     9241 ataggggttt ctgtgaagta gtgaggaaga aggagttttg cagatggata ttcgattggc
     9301 acgtatagat gatcgtttaa tccacgggca agtcgcaaca gtttggtcga agcaaacagg
     9361 tgttgaacgt atcattgtga tcagcgatgt agtcgctcaa gataaattga gaaagtttct
     9421 actaaaagaa gctgcaccgc cggggatcac agcaaatgta ataaccgtgc agaaaatgat
     9481 tcaaatctat catagtggat tattatcacg agcaaaagtg atgcttttat tcaccaatcc
     9541 ccaagatgtt gaagcggttg tccgctgcgg tgtacagttg aagtcgttga atatcggcgg
     9601 tatgagtttt actgaaggca aatcaatgat taccaatttt gtttcagtaa atcaacaaga
     9661 tattgattcc tttcgttact tgaacagtca agaagtggag ttagagattc gaaaagtacc
     9721 tgctgacagg aaaatccttt tgatggaact actagcgaaa atcaaaaagg tgtagcaaga
     9781 atggttgttg gaaaaggtca aataattctg ttgtaatggt tagcaattat taatattttt
     9841 gaatttgtta caacaaaagt aaataggagg tataaatatg gtagggatta tcctagcaag
     9901 tcatggacaa tttgctgaag gaatcttaca atccggttca atgatctttg gcgaacaaga
     9961 aaacgtcaaa gccgtgattt tgaaaccaag tgaaggtcca gatgacttga gagccaaatt
    10021 ggaagaagcg gttgcttctt ttgataatca agacgaagtg ttattcttag ttgatttatg
    10081 gggcggaaca ccgttcaatc aatcaaacac attattcgaa gagcacaaag ataaatgggc
    10141 aatcgtcagc ggtttgaatt taccaatgtt gattgaagcc tacgcatcac gcttttcaat
    10201 ggaatctgct catgaaattg cagcacacat cattgaaaca gcaaaagacg gtgtaaaagt
    10261 aaaaccggaa gaattagaac cggcagaagc accgaaagca gcagttgaag acgcacaacc
    10321 taaaggagca cttccagaag gaactgtggt cggagacgga aaaatcaaat atgtattagc
    10381 acgtgttgac tcacgtctgt tacatggaca agttgcgaca gcttggacaa aagcagtgca
    10441 accaaaccgt attatcgttg tttcagatgc agtatcaaag gatgatctgc gtaagagact
    10501 aatcgaacaa gctgctcctc caggtgtaaa agcaaatgtt attcctatta gcaaaatgat
    10561 cgaagtagca aaagaccctc gttttggaaa taccaaagca ttgcttttat ttgaaaatcc
    10621 tgaagacgta ttgacagctg ttgaaggtgg cgtagacatc aaagaattaa atgttgggtc
    10681 aatggcgcat tcggtcggta aagtcgttgt aagtaaagta ttgtctatgg gacaagaaga
    10741 cgttgaagcg tttgagaagc ttgaacaaaa aggtgtgaaa ttcgatgtac gtaaagtacc
    10801 taatgattca cgcgataaca tggatgatat tttgaaaaaa gcaaaagctg aattagcaaa
    10861 agcgtaataa ataatattaa aatttatagg aggcttatca tgtctattat ttcaataatt
    10921 ttagtcgtat tagttgcctt tctagctggt atggaaggta ttcttgatga atttcaattt
    10981 catcaaccac tagtggcatg tacattgatc ggtttagtta ccggtaacct cgaagctggt
    11041 atcgtacttg gcggtacgct tcagatgatc gctttaggtt gggcaaatat cggtgccgct
    11101 gtagcaccag atgctgcatt agcatcagta gcatcagcaa ttattttagt tttaggtgga
    11161 caaggtgtta aaggtgttcc ttctgcgatc gcgattgccg ttcctttggc tgttgcaggg
    11221 ttgttcttaa caatgatcgt acgtacaatc gctgttccaa tcgttcattt gatggatgcg
    11281 gctgctgaag aaggaaacat ccgtaaggtt gaaatgtggc acatcattgc tgtatgttta
    11341 caaggggttc gtatcgcggt cccagcagca gcgttgctat ttatcccagc acaaactgtg
    11401 caatcattcc ttgaatcaat gccagcatgg ttaacagatg gtatggctat cggtggggga
    11461 atggtcgtag ctgtaggtta cgctttggta atcaacatga tggctacaaa agaagtatgg
    11521 ccattcttcg ttattggttt cgttgtagct gcaatttctc aattaacatt gattgctcta
    11581 ggtgctttag gtgttgcatt agctcttatt tacttgaacc tttctaaaat gggtggttca
    11641 tcaaataatg gaggcggaag caactctggt gaccctttag gcgacatttt gaatgactat
    11701 taatcttgaa ggaggaaaat aaaatggcag aagaaaaaat caaattatca aaaaaagatc
    11761 gtttagctgt tgcgtggcgc tctaccttca ttcaaggttc atggaactac gaacgtatgc
    11821 aaaacggtgg ctgggcattt tctatgattc cagcaatcaa aaaattatac aaaacaaaag
    11881 aagaacgttc tgcagcatta aaacgtcact tggaattctt taatacacac ccatacattg
    11941 cttctccaat tttaggggtt acattggctc tggaagaaga acgtgcgaat ggcgcgcctg
    12001 ttgatgatgt agcgattcaa ggggttaaag taggtatgat gggtccttta gctggtgttg
    12061 gtgacccagt gttctggttt acagtacgtc caatgctagg tgcactaggt gcttcattag
    12121 ctatgggagg aaatatcctt ggaccaatca tcttcttctt aggttggaac attattcgtt
    12181 gggcattcat gtggtataca caagaatttg gctacaaagc tggttctaaa attactgatg
    12241 acctttctgg cggattgtta caagacgtaa caaaaggtgc ttcaatttta gggatgttcg
    12301 tattagctgc ccttgtacaa agatgggtat caatcaaatt cttaccagtt gtatcaactg
    12361 taaaattaga taaaggtgct tacatcgaat gggataaatt gccttcaggt ggtgaaggaa
    12421 tcaagagtgc atttgaacaa gttaataatg gcttagccct ttctcctgaa aaggtaacaa
    12481 cattacaaga caacttagac caattgattc ctggtttagc tgcattagga ttaacattgt
    12541 tatgtatgtg gttgcttaag aaaaaggttt ctccaatcgt tatcatcctt ggtttattcg
    12601 tagtcggtgt tgttggacac gtcatcggac ttttataatc cataaaaata tagctcgggc
    12661 gttgtcccga gcttttttta tatagtatac tgttcataaa caaatatctg gagggagaaa
    12721 atggttcaat caatcaatac aaaagtagac ttggtaattg atgctacttc gtttacgggt
    12781 ctgacagatt acgggcaaat catgataggt aataatggtt ttgagtttta ccatgcgcga
    12841 gatagacgca aatatatcca aattccatgg gaagaagtgg attatgtact agcatcagtc
    12901 atgctaaagg gaaaatggat tcctcgatat gccattcaaa cgaaaaaaaa tggtacatac
    12961 acattttctt ccaaagaacc taaaaaagtg cttcgtgcga tcagagaata tgtagatgaa
    13021 gatagaatgg tacaatcatt gagtttcttt gatgtagtga aacgtggtat tcgtagtatg
    13081 tttaaacgca agtaaaaaat aaagaaaact tctaagagga ggttttcttt atttttttac
    13141 tttgcagaag aaaatatgaa atagcaaaac caattatttg tcccctttaa atcccatatg
    13201 ctaaaattga ttagcaaaga aggaggttat attatgagtt ggttatggac attgattgtt
    13261 ggagccgtga tcggagcaat tgcaggtgcg atcaccagta aaggaaattc gatgggctgt
    13321 gtaacgaata tcattgctgg actagtcggt tcaagtattg gacaagcgat tttcggcgaa
    13381 tggggaccaa aagctgcagg tatggcactt attccatcta ttttaggcgc agtgattttg
    13441 gtagcagttg tctcattttt ctttggaaaa cgatcataag tttttacaaa atagtaaact
    13501 aaaaggagtt gcagcgagac tgtgactcct ttttggcttt atttatttaa aataaaaaga
    13561 agttttccgg aaatttttat ttaactgtcg tgtacgaaga aaagtttgct ataatggaaa
    13621 aaagcgtgca aagaaagagg gagtttcatt atgatgctac gtgtagaaaa attgcgcaaa
    13681 aaaatgcaag aagaaaattt ggattcattt ttggttacca gtccgtacaa tcttcgttac
    13741 ttaacgaact ttacaggtac gacaggatta gctgttatta ctttggaaaa agcatttttt
    13801 attactgatt ttcgttatac cgagcaggcg gcagcgcagg ctcaagggtt tgaaattatt
    13861 aaaaatgtcg gacctatttt tgatgaggta gcaaacttag tacgcaaaga agaactgcac
    13921 tcattagctt ttgaggaaac aacagtctca tttttagaat attcggtttt agaagaaatc
    13981 atcgatgcgg aattagtacc tgtttcacag atgatcgaag aactcagaga agtgaaagat
    14041 gaagaagaaa tcgctattat cgaaaaagca tgtcatattg cggatatggc atatgatcac
    14101 atcctgaaaa tgatccaacc gggcatgact gagattgaag tcgcaaatca actagacttt
    14161 tatatgcgtt ctcttggcgc ttcaggtgtt tcttttgata cgatcgttgc tagcggattg
    14221 cgttcggcta tgccgcatgg tgtagcaagc aaaaaaatca tcgaacaagg tgacttgatc
    14281 acattggact ttggttgcta ttacgaaggg tacgtttccg atatgacacg tacattcgct
    14341 attggtgatc ctggggaaaa attaaaagaa atttatcaaa tagtcttaga tgctcaatta
    14401 gcagttattg atgctgcaaa accaggaatg actggtgtcc aattagatgc agttgctaga
    14461 gattatattt caaaacatgg ttacggggaa gcatttggac attctacagg acatgggatc
    14521 ggtttagaga tccacgaagg accaaacgtt tcttctcgtg cagaaaagca atttgtacca
    14581 ggaaatgtga tcactgatga accagggatc tacttaccag gaatcggtgg agtacgtatt
    14641 gaggatgact tgttgatcac agaagatgga aatcgtgtgt tgactcgttc accaaaagaa
    14701 ttgatcatct tatagtttca tcaatagaag aagtaagcgt aaaaatcagt gttcacagac
    14761 tttgttatag aaaatcactc gatttaatga tacactatag aagaactttt aagctgtaag
    14821 agataataaa gttgggtgtt ttctccaatt catagcagtt tgcagctgat cggcggcagt
    14881 tttcttacca cgtgatcact atgccgcatc atagagaatt cggatgtatc tcgtgcttag
    14941 gcaaagggag gaataaataa atggcagatg aaaaaaattt gattttaaac gcaaaccaag
    15001 atttaggaga gatcgtgatc gctcctgaag tcatcgaagt aattatcggg attgcagctt
    15061 ctaaagtaga aggcgtatac ggaatgcgcg gaacttttgc cagcaatgtc actgaattat
    15121 taggacgtgc agctcacgga aaaggtgttt acttagcaaa cgaggaagat ggcttgaaag
    15181 tagatcttta ctgttatcta gaatatggtg tttcagttcc taaagtagca atggaaatgc
    15241 aggaacgggt cagccagcaa gtccttttca tgacggatat cgatttatct gaagtcaata
    15301 tccatgtagt agctgttgtt cctgaaaaat tagaacaacc aacaatcgaa gaactttttg
    15361 aagacgaaga ggaagagaat gaataaagaa ttatcaagac acgagattcg ggaaatggca
    15421 ttgcaggctt tatttccatt agacttcaat gcagacctaa caaaagaaga tgcgattttc
    15481 aacgcaatcg aattagacca ccgcgacatg atcaacgaag atgaatcaga gtttgttcct
    15541 gtttatcttg atacgctagt tggcggtgtc tgtgcaaaaa aagatgaatt ggataaagtg
    15601 attgaaaaac atttaaaaaa caattggcac atcaatcgga tctcaaaaat ggatttggtg
    15661 atcttgcgta tcgcgatttt tgaaatgact tatgtgacag atgtaccagc ggctgttgct
    15721 ttaaatgaag caatcgagtt ggcaaaaaca tttagtgatg accgttcacg taaattcgtc
    15781 aacggtgttt tatccaatgt attgaaagaa ttagaagctg gggcgtaagt cctagctttt
    15841 ttctatgaat catcataaac ctgttataaa tcgggaaaat tctatggtag aatgaaaaca
    15901 caaaaaatat ctgggagtgg ttagatggca gaattgatta acggaaaaga attagctgaa
    15961 aaaatgcagg cagagattgc tgaaaaaatc caaaaattga aagataatgg tatccatccc
    16021 ggattagtcg ttttattggt aggggaaaat ccagccagcc aaatctatgt aagaaataaa
    16081 gaacgtgtag caaaagaaat cggaatccat tcattggtag agcgttatcc tgagacgatt
    16141 tctgaagacg aactacttgc agaaattgaa aaatacaatc aagatcctag ttttcatggt
    16201 atattggtcc aattaccatt gccaaagcat atagatgaag aaaagatcct gttagctatc
    16261 cgccctgaaa aagacgtaga cggttttcat ccgatgaatt taggcagact ccttgcgggt
    16321 aaaccggaca agattccttg tactccatac ggcatcatga aaatgtttga agcttattct
    16381 atcgatccgg cgggtaaacg agcagtagtt atcggacgaa gcaatatcgt cggtaaaccg
    16441 atggctcagc tactcttgat ggcagatgca actgttactg ttgctcattc taaaaccgaa
    16501 aatctggctg aactggcaca agaagcagat attttagtcg tagcaatagg acgtggacat
    16561 tttgtcacaa aagaattcgt aaaaccaggt gctgtagtga tagatgtagg gatgaatcgt
    16621 gatgctaacg gtaagctgat cggtgatgtg aaatttgatg aagtggagcc tattgcaggc
    16681 tggatcactc ctgtgccaaa gggtgtaggg ccaatgacta tcacgatgct gatgtatcaa
    16741 accgtaaaat ctgccgaagc ttttggagca ggtgaataga tggcacaaga atatttaact
    16801 gtcactacat taacgaagta tttgaaaaga aaatttgatg ccgatcctta tttagaacgc
    16861 gtatatttaa caggcgaaat atcgaatttc cgtttacgtg ccaatgccca tcaatatttc
    16921 agcttaaaag atgaccatgc aaaaatatct gctattatgt tcaaaggagc attccaaaaa
    16981 ttacgattcc agccaaaaga aggaatgaaa gtccttgtcg ttgggcgtat ttcattatat
    17041 gaagcgggag gttcttatca aatctatgta gagcacatgg agcctgatgg aataggtgct
    17101 ttatatcaag aattggaaga acgaaaagaa aagctgatga aggaaggcct ttttcaaggg
    17161 cctaaaaaaa tgctgccacg tttcccaaag cgaatcgcgg tccttaccag tccaagcgga
    17221 gcggttatcc gcgatatcat cacgacggtg aaaagaagat atccaattgt ccaattggtt
    17281 ttgttcccaa cagttgtaca aggaaaccaa gcagcggaca atgttgtcag gaatatccag
    17341 agagtagaag agatgggcaa tttcgataca atgatcatcg gtcgtggggg tggttcgatt
    17401 gaggatttat ggccgtttaa tgaagagcgg gttgctcggg cgatttatca agctaatacg
    17461 ccggtgattt catctgtagg acatgaaaca gataccacga tcgctgattt agtagcagat
    17521 gttcgggctg ccactccaac agcagctgca gaattagctg ttcctgtttt gtcagaagag
    17581 ttgatgcgca ttcaagaacg gcaagctaga ttgcagcagg cgtttgtacg ccagatccag
    17641 cgcaaacaag aacggttcga gcgtgctcga cgttcctatg tatttcggca gcctgaaaga
    17701 ttgtatgaag gtcagaccat caaattagac cagttgaaac agcgattata tcaagctaca
    17761 cagcaaattt atcatgcgaa agaaaaacaa acgatcgcat taagccacca attggaacaa
    17821 gtggctccgc tttatcgagt taaagcagct cgccaagaaa ctgcttatct agaaaaacgc
    17881 ttagaagaga aaatccaaca atatatgaaa caacagcagc agcgttttgc tcaagctgtt
    17941 caatctcttg atttgttgag tccattgaaa atcatgggaa gaggctacag ctatacgacg
    18001 ttaaatgatc aagtagtaaa aagtgtcacc gagattcatc aaggggattt gctgacgatc
    18061 cattatcaag atggaatcgt taaaggacaa gtacaagaga tagacaaagg agaataaaaa
    18121 tatggaaaat ccaacttttg aagagtcact gcaagaatta gaaaaaattg tcatgcagtt
    18181 agaacaagga gacgttccat tagaatcagc tctagattcg tttaaacgag gaatggaatt
    18241 aagcaaacat tgccaagata cattagctaa agcagaagag accttgacga aaatgatgac
    18301 agaatcaaat gaagaagttg attttgacgg aaacgaggaa agctgagatg agattaactg
    18361 acttttcagc tattcatcta cctgctgtag aaaaagaaat cttgtctttt ttagatgagc
    18421 atacaacgga tagaggactc tatgatgcga tgagttattc agtgaaagca ggcgggaaaa
    18481 gaatacgtcc cttattactt ttaacggccg ttgcttcctt tgatgagcct attgatgttc
    18541 ccgtctatca agtagcagct gctttagaaa tggtgcatac gtattccttg atccacgatg
    18601 atttgcctgc gatggacaat gatgatcttc gtagaggaaa acctaccaat cataaagtat
    18661 tcggcgaagc attagctata ttggccggag atggtttgct tacaggagca ttccagctaa
    18721 tcagtatggc acacttaggt aattctccta agcttctgct gcttcagcaa ttagctgttt
    18781 gtgcgggatc acaaggtatg gttgctgggc aagctgcaga tatcgagggc gaatcgaaaa
    18841 agctttcttt agaagaatta gcatttattc atgaaagaaa gacagggcat ttgattcgtt
    18901 atgccttgct ggctggtggc atattagcga agcaacctga agaaatctta ttgctacttc
    18961 aacgattagc cgaacattta ggtttagctt ttcagataag agatgatcta ctcgatgtga
    19021 ttggtacaac gaaaacatta gggaaaacag ctggtaaaga tgaacgtatg gaaaaaaata
    19081 cttacccgcg tttattaggc ctagaaaaga cacgtgaggc gttggagatt gagctgttat
    19141 ccgccaacaa gatcatagac aaattagaag aggacgtttt ttcttttgat ggcgggctga
    19201 tgcgccagat gatcaagcaa tttgatgtgg agaaatgagg gaaagaatgg aaaaagaacg
    19261 cgtagatgta ttagcggtca aacaaggatt atttgaaaca agagaacaag ccaaacgttc
    19321 agtgatggct ggtttgattt acaacgaaaa aaacgagcgg tttgataaac cgggagaaaa
    19381 aattccagtg agtagtgaat taagagtcaa aggtaaaaaa cttccttatg tttctcgagg
    19441 gggattaaaa ttagaaaaag cactcaaagc atttgatcta tctgttgaag gaaaaacatt
    19501 gttagatatt ggtgcatcca ctggcggatt tactgatgca gctttgcaaa atggcgcaaa
    19561 aatgagttat gctttagacg ttggctacaa tcagctagct tggaaaataa gacaagatcc
    19621 tagagtcgtc gtaatggaaa gagtgaattt tcgttatgca aaacccgaag actttacctt
    19681 aggaattcca gaagttgctg ttatcgatgt ttcttttatt tcactaaaac tgatgctgcc
    19741 tccattacat gctattttaa aagaagaggg agaagtgatt gctttaatca aaccccaatt
    19801 cgaagcaggc agagaagctg tagggaaaaa cggaattgtt cgtgaccctc agacccatca
    19861 aaaagtttta gaagacattt tgtcatttgc ggcacaaggt ggatatgatg tgctggaatt
    19921 aagttattct ccgatcacgg ggggagaagg aaatatcgag ttcttggctc acttgaggaa
    19981 agtacccgaa agcggaacaa tcaattcagc tattaatatg gcagaagtgg tttcaaatgc
    20041 acacgaacag tttgaccata aataaattgt aactgtgaaa atcaagtgag aagtatgcgg
    20101 attcttcttt attataccaa ggaaataagc tatcattgat taagaagaat atgctaagga
    20161 gcgaagacgt aatgagaaaa aaagatcggc atcgtttgat tactcgattg ttgagtgagc
    20221 aagatatccg aaagcaggaa gaatttgttg aaatcctgaa aagcaaagga atatctgtta
    20281 ctcaagctac gatttccaga gatataaagg aactgaaact gatcaaggta ccggcttcag
    20341 atggcggcta ccgttatagc ataccagctg aaacaaacga agatgtaaat actaaattgg
    20401 aaaaactatt aaaagacgct ttcgtggcag ttgaccaaat ggaaaaattt gttattttga
    20461 aaacattgcc gggaaatgct tcggcagcag caaatttgat tgataaacgt tacaagaaag
    20521 aattattttc gattatcaat gatgacgata atgttttgat gattacacga actgaagacc
    20581 aggcagtttt attgaagaaa gacttcctgc attaccttta agttgaggag cgaatgtgat
    20641 gctacaagaa atcagtatta ctaactttgc gattattccg gaattgcggc tttcttttca
    20701 cgaaggaatg accgcgctga ccggagagac tggtgcgggt aaatccatta ttatcgatgc
    20761 tttaggtctg ttagcaggag gaagaggttc aagcgattat atacgtcaag gagcagaaaa
    20821 atgtgtgctt gaagggttgt ttgaactacc aaaacaagaa ggattctctg aactgatggt
    20881 tgaattaggg atcgaaacag atgaggacaa tctgattgtt cgacgggata tgtccttaac
    20941 tggaaaaaat gtttgtcgag tgaatggtca tattatcaca ttagctaatc tgagaagaat
    21001 cggcagttat ttagtagata tccaaggaca aaacgaacat caggaactgc tccagcctga
    21061 atcacatctt gctttattgg atcgttttgg agatgtagct ttccaacaga agaaaaagag
    21121 ttatcagcaa gaatatatct cttatcgtga gctggaaaaa agggtacgaa aagtgcagca
    21181 aaatgaaaaa tcctatgttc agagaataga tatgctccat ttccagcaag aagaaatagc
    21241 tgctgctgat ttgcaagtcg gagaagaaga gaaattaaaa gaagaaagag aaaaacttag
    21301 caattatcaa aaaattgctg atggattagc tgcaggttac ggagcactga cgaataatga
    21361 acaaaacagt gtagatggtg ttggcttggc cgtttcagaa ctgcaaggga tcgcccatct
    21421 agatgtggag tatgaagcaa tttatgaaaa tatccaaagt gcttactatt tgctgcaaga
    21481 tgcaatcggc gatatgagtc gtcagattga tttattggag ttagatgaga gtcgtttaga
    21541 agaagttacg caacgtttag aattaatccg acagttgaaa cgtaaatacg gcgaatccat
    21601 tgagtcaata ttggcttact atgacgaaat caccgaagag ctagcttctt ctgatttttc
    21661 agaaggtcaa ttggacaaaa tgaaagagga actagagcaa aaagagcttc tacttcagca
    21721 acaagccgca gatcttcatg aagcgcgtaa aaaaatcgca aaagaattgg aaaaatccat
    21781 tctgcatgaa ttgaaaagtc tgtatatgga aaacactgag tttgaggtac gtttcttaaa
    21841 agaagaaaat agacaattga atcatgatgg atttgaccaa atcgaatttt acattacgac
    21901 caatccagga gagccactga agcctttggt gaaagtcgct tctggtggcg aactgtctcg
    21961 gatgttgctt gctttgaaaa ctattttttc ttctgagcaa ggtgtgacaa gtattatttt
    22021 tgatgaagtg gatacaggtg tgagcggaag agttgcacag gccatcgcag ataaaatttc
    22081 tcaaatatcg aaatactctc aagttttgtg catcactcat ctgcctcaag ttgctgcagt
    22141 cgccgactat caatattata ttgtgaaagc agtgattgat ggacgtacgc aaacttctgt
    22201 ttcagagttg aaaacaaaag aaagagaaga agaaattgca cgtatgctgg ctggaagtga
    22261 gattacaaaa ctcacactcg aacatgcaaa agaactattg aaactcgcaa aaaggacctg
    22321 agccacctcg cagtggttca ggtccttttc agcagctgat agctatattt tatttttaca
    22381 tgacactcat tacagcagtc aatacaacag atccaatcgt taaaatcagc attgtccaaa
    22441 tgacaaattt tgttacttta gcaaaagtac ttgtttgttt tttttcacgc attgttttca
    22501 acctcgtcat taattagttg attcaagtgt acaatctttt ttccatttcc acaagagggc
    22561 ttttttataa aaacttccct ttttttaaat aataggtagt tacgatacaa ataaataatg
    22621 ttaatagaac tagccatata aaggcatcat ccctttcagc aataggtaac ttaatgttca
    22681 ttccatagat actgccgacg attgtcggta tagttaaaat aatcgttaaa gaagttaaaa
    22741 ttttcataat attattcaga ttattcgata cgattgctga aaaagtctga ttcatttgtt
    22801 ctactagttt taattgaatg cgagaagtag tcattgcctg ctctgtttcc accaatacat
    22861 cacgtaaatg atttttcaaa gcatgatttt cttggaaagg tttattttgc gctagttctt
    22921 tcaatgcatc taaattagca gaagtcgctt ctctgaaata aacgaggctt ttttggatat
    22981 ccatgatttg ataaagttgt ttgttttcag tggatacttg aagtttggct tccaaagtat
    23041 cgcattgaga ttgaagcgta gctaaataac tgttgtaaga gagtgtgacc tgccaaatga
    23101 attgaaagaa gatattgaga ttaggcgaaa tatcactttt tggaaaggtc tggctaaaga
    23161 ctttctttaa aaacagtggt tcatgattcg taattgtgat aagcttcttt ttgggcgtca
    23221 aaataataga gagcgggtat gtatccactt gaaaataacc gctaggactt gttttagcaa
    23281 aaggatattg taacaataat aacgctggct ttgtcaactt tgattgattg aattcttctg
    23341 tacgggaatt ttctgcatcg tccaatatgg aagtgatgta atctgacgga atttcaaatt
    23401 gatgtgtcag ctgatgaatc tcttcttgat taggacgttc tacacaatac cagatcgttt
    23461 ggtcagtttc ctttccggta ttacgcagtt tctcattatc taatgtaaaa tatttgatca
    23521 tgattttcct cctgttgcct gtttctttta ttataacgga tagggaattg gaatacatgg
    23581 aatgtgtttg taagatatag aatataactg cataaattta aataaaatga aacagttgtg
    23641 taaagtatat acagtatact tgaagcaacg ataaaaaagg agtttacgtt catgggcaca
    23701 ttcacaccac ttatcttgtt ttggtatatt tacccggtaa tcgttttgtt tggttgccaa
    23761 tttcttgtaa aattattttc gttaaatcgt cgttttaaaa taaaagcccc tgatttggcg
    23821 attccttttt tgtggggcgg tatgcatgca ttgtctagaa atacagggac aatttctatc
    23881 ttaccgtttt tctttatttc tgttttgcta atgggaattt gtattgctgt tttccaagca
    23941 tattattatg aagaaattat ttatccgcgt tatttcaaga tgacttggcg tttggttttt
    24001 ctgttgacaa tcgtcttgta cgctgtcttg atcattgtga acatactttc ttacctatga
    24061 aaaaaataga aaagctgtaa aaagtctttt aaaaggcttt ttgcagcttg atttttaaaa
    24121 aaatgtttat gaataaaaaa agttgttccc ggatttccca gaaaaggtgg tagaaagtgg
    24181 gggattgtgg tagactaatt ttagttagtg gaggaatggg gggctttcaa tgttgatggg
    24241 tgaattccaa cataatattg atgcaaaagg acgtttaatc gtgccctcaa aacttcgtga
    24301 agaacttggc gaaaagtttg ttctgacacg cggattggat ggctgtcttt tcggctatcc
    24361 gatgagcgaa tgggaaaatc tggaagctaa actgaacgaa atgccgttgg ctaaaaaaga
    24421 cgcacgtact tttgtgcgat tcttctattc ggctgccact gaatgcgaac ttgataaaca
    24481 aggacgaatc aatataccca gcacgttaag aaattatgca gctttgacaa aaggttgcgt
    24541 agtcatcggt gtatctaacc gaattgaaat ttgggatgag acacgatggc aagatttttc
    24601 tgcagcagca gaagagaact ttgacgaaat tgctgaaagt atgattgatt tcggactata
    24661 agaataggaa gaggctgact aatgactgaa acatttcaac actatacagt catgttgaaa
    24721 gaaacggtag atggattgaa catcaaaccg gatggcgttt atgtagactg tacactcggc
    24781 ggtgccggac atagtgaata tttattaagc caactaagtt ctaaagggca tctgtatgcg
    24841 tttgaccaag atcaaaaagc aatcgatcac gccaagctgc gtttagcttc ttatgtcgaa
    24901 caaggtatgg tcacatttat caaagcgaac ttcagagaat tagaagaacg cttgagtgag
    24961 catgtagata aggtagacgg cattttgtat gatctagggg tttcctctcc tcagctagat
    25021 gaagcagaac gtggatttag ttatcatcaa gatgcaccgt tggatatgcg catggatcag
    25081 tcagctcctt tatccgctta tcacgtaatt aatgaatatt cctaccatga attagtaaag
    25141 attttcttcc gctatggcga ggagaaattt tccaaacaaa ttgctcgtga aatcgaacga
    25201 gtacgaaaaa caaaaccaat ccaaacaact ggtgagcttg tcgaaatcat taaacaagtc
    25261 atcccagctc ccgcaagaag aaaaggcggg catcctgcaa aaagaatttt tcaagctgtg
    25321 cggattgcag taaatgacga actcggtgcc gaagaggcct ctttagaaca ggcaatccgg
    25381 ttattgaaag tcaatggaag aatcagcgta atcacattcc attcattgga agacagaatt
    25441 gtcaaaagta tgtttaaaga atatagtacg gtacaagatc taccaccggg acttcctgtc
    25501 atacctgacg aattccaacc agaactgaaa gtcattactc gtaaaccaat ccttccttca
    25561 gaggaagagc taacagaaaa caatcgatca cgcagtgcaa agttacgtat tgcagaaaaa
    25621 ataagaatga aagaggagta gaatgatggc agaattaaag aaaatgcaag attacgaata
    25681 tgatttacct gaattggtgg atcagccgga acaacaaatg cctgattctt cttcgaatca
    25741 agaaatcatt cttccacaat caccggcaaa acgattaaag cagatttctg ctttggaaaa
    25801 aattgctgtg gcaagcgtgg cgattgctat tgttgcttta tgtgttttaa cagttatgct
    25861 gcgcacaaat atcagcggag ctgaaaaaga aatcacgtca atacaaacag aaatcaatga
    25921 taaaaagcaa gaaaaaacga gtttagaaca agaaaaaaac gaactttctc gtactgaacg
    25981 gattaaacaa atcgcagaag aaaaaggatt gtctattaat gacgacaatt taaggaaagt
    26041 gaagtaaatg agtcttaaaa ataaaattcg gcggtttatg gaaaagaaaa atctaaatcc
    26101 gatgaacaat cgcaaaaaag taggtatcat tttatttgct acgagtattg gattgttctt
    26161 tttatttgcc gtccgtttct cgtatatcgt tattggaggt catgttgctg gtacctcatt
    26221 agctgaaaaa acaaaacagt tgtaccaagg aagtgaagta gtaaaagcaa aaagaggaac
    26281 gatttatgat cgtaacggtg tggcgctcgc agaagatgcc tcctcttatt caattaaagc
    26341 gatactctca aaaacatata cctctgggga taaaaaactg tatgttgaag aaaagaattt
    26401 tgataaaata gcagaaatcc tgcataagaa tctttctata gacaaaaaag atgcactgaa
    26461 tattttagag gacggcgcaa aaaaagagct gtatcaagtt gaatttggta gctatgggaa
    26521 aaatatctcc caagaaacca agcaaaacat cgaagcggac atgaaaaaag aaggtgtcgc
    26581 tggtctttat tttgtcgacc atcaggctcg gatgtatcca aatggcgtct tttcttctca
    26641 ttttattgga tacgcagttc cagacaaaga tgagaatggc ttagttggta aactaggttt
    26701 ggagtctgct tacaatgata tcttgagcgg taaagatggt aagattattt atcaaaaaga
    26761 taattttcaa aatcctctgc caggtacagt ggcagaagaa gaaaaagctg tcgacggaca
    26821 agatatctac acgactttag acagcagact acaaagttat ttagaaacac tgatggatca
    26881 agtaaatgaa gaatatcagc cagaagaatt gacagcagtc ctgatgaaag caaaaaccgg
    26941 agaaatcttg gcaatgggac aacgacctac tttcaatcct gaaacaatgg aaggattgac
    27001 tggaaaagat gctatctggc gtaatttctt ggtacaagat agttatgaac caggatcaac
    27061 catgaaagtt ttcactactg cagctgctat cgaagaagga gaattcaacg agaatgaaac
    27121 cttccaatct ggaaaaatcc aagtagaaga tgctacgatc aatgaccatg actttggtga
    27181 aaaaggtgtg ctgaccatgc gtcaagctct ttcatggtca agtaacgtcg ggatggtcat
    27241 actggaacag cgattaggcg gacgatggta taactattta caaaaactgg gatttggaca
    27301 aagtactcat tctggattag atgatgaagt aaacggtgct ttacctacat taaatattgt
    27361 cgaccgagca atgagtgctt atggacaagc ggtaggtgtg acgaatttcc aaatgatgaa
    27421 agctttcact tctattgcga ataacggtac aatgattcaa cctcgatata tcagcaaagt
    27481 ggtggatccc caaactggag aagagcgaac aactcaaaca gaagttctag ggcaaccttt
    27541 ttcaaaagaa acaacagaaa aagtccgtga atatatgaga gatgttgtcg aaagcgaaaa
    27601 ctacggtagt gcttatggtg tatatagtgt acctggatac aatgtatcgg caaaaaacgg
    27661 aacagcccaa atcgcttctg atacaggggg atatcaaaca ggagatacag cctacctgta
    27721 ttcgattgtg gagatggttc cttctgaaga tccagactat gtcttgtatc taacaatgaa
    27781 acaccctaag acatatgatc gaatggcatt ggctaaaatc gcaaatccat tgatgaaacg
    27841 ggcaatggat tttaaagaaa ctgaagaaga ttcagataca gaaacaaaaa cagaaaaagt
    27901 atccgtagcc gattatcgta atcttgaagc ggacgttgca gcagctgatg ctcaaaaaag
    27961 tggactgcaa cctgtagtga tcggtaatgg taaaaaagta caaaaacaat ctactgccaa
    28021 cggtgatcag ttgatctctg gagaaaaact gatcctatat actggtggtg ataagctgat
    28081 gcctgacgta acaggatggt caaaagcaga tatcatgaag ctggggaaaa tcctcggtat
    28141 tgaagttagt tttgatggag atggttattg tgtcaaacaa gaacttgctc catatgaaaa
    28201 aatcactgaa gacaaactta gttttacttt agaagaataa gaagagaaat aggagagaaa
    28261 agagcatgga atggacacaa gcattgattc ccattgtcag cagttgtgca atgacaattg
    28321 ccgcaatgcc gctatttatt ggttattttc aaatgaaaaa gcaaggtcag gcgattcgtg
    28381 aggagggacc aaagtggcac aatagcaaag ccggaactcc tacaatgggc ggcttagtgt
    28441 ttctgatcgg ctctattttg acaggaattt gggttggagc ttggcaaaaa cagctaactc
    28501 ctactctttt catcttgctt tttgtattag cgttatacgg agtaatcggc tttcttgatg
    28561 attttataaa aatattcaag aaaagaaaca tgggactgaa ctcgaagcag aaattgattg
    28621 gacagattct agggggaatc atcttttatc ttgtttaccg ttctgaaggt tatccaggtg
    28681 tattgaattt cttcggacta gatctacctt taggaatcat ttatggtatt ttcgctctct
    28741 tctggcttgt tggattttca aatgcagtaa atctgacaga tggcatcgat ggattagttg
    28801 ctggcttagg aagtatctct tttgcagctt acggtctaat tgcttggcat cagcatcagt
    28861 atgatgtatt aatcatttgc ttaagtgttt taggaggttt actgggtttc ttcgtctaca
    28921 acagaaaacc tgccaagatt tttatgggag atgtcggttc attagcgttg ggcggacttt
    28981 tagcggcaat ttctataatg ctaaaccaag aatggacatt gcttttgatt ggtttgatct
    29041 atgttatgga aacagcaagt gtgatgcttc aagttacttc ctttaagctt acaggaaaac
    29101 gaatttttaa aatgtctcca attcatcatc actttgaaat gtgcggatgg tcggagtgga
    29161 agattgatac agttttttgg ttgatcagca ttgtgacttc gttgatcaca ttatggatcg
    29221 tttggtagga gatgacggga aatgaaaaaa atatcaacat atgaaaataa gaaagtgctt
    29281 gtcttaggac tcgcaaaaag cggagtaagt gcggccaaat tattacatga attgggcgca
    29341 cttgtcaccg ttaatgatgg aaaacctttt gacgaaaatc cggaagcaca agagctgctt
    29401 tctttaggga taaaagtaat aacaggtagt catccaatcg aattgttgga tgaagaattt
    29461 tcattgatgg tcaaaaatcc agggattccc tacagtcatc ctttagtagc aaaagcccaa
    29521 gaaatgggta ttccggtaat tacggaagtt gagctagctt acgaagtagc ggaatgccca
    29581 attattggga ttacaggaac gaacggaaaa acaacgacaa caacgatgac aggactacta
    29641 ctgaatgcag gagcagatca aggaattgca cgtttagcgg gtaatattgg gtatccagca
    29701 agtggtgtag cgcaagaagc aaaatcagaa gataaaattg tgatggaatt gtctagcttc
    29761 caactcatgg gaatcacaga tttccgccct catatagcgg tgattaccaa tatctatgaa
    29821 gcacatattg attatcatgg tacaagaaaa gaatatgtca aagctaaatg gaaccttcaa
    29881 aaaaacatga cagaaaaaga ttatctgatc cttaactgga atcagtcaga actacaagaa
    29941 ttagcgcaaa gaacaaaagc acgcgtactg cctttctcga caaaagaagt gttggaagac
    30001 ggtgtgtatg ctgacgatta ctcaatatac tataaaaaag aaaaaataat ggaaatatct
    30061 gaacttggcg taccaggtaa gcataatgta gaaaatgcac tagctgctat ttctgtagca
    30121 aaattgtatg gtatatcaaa tgaagctata cgagaaacgt tgcatttttt ccatggcgta
    30181 ccacatcgta cgcaatatgt aggagaaatc caaggcagaa aattttataa tgattcaaaa
    30241 gcaaccaata ttttagcgac taaaatggct ctaagtggat ttgaaacgtc taaggtcgtg
    30301 ttattggctg gcggattaga cagaggaaat acatttgatg aattaattcc ttcattaaaa
    30361 ggaatcaaag caatggtcgt atttggccaa acaaaagaaa agttgatgga tgctggaaaa
    30421 aaagcaggca tcgagacaat agtaacagcc gattctgtag aacaagcagt tcctttagca
    30481 ttagaaaaca gtacagatgg tgatgtagtt ttattatcac cagcgaatgc cagctgggat
    30541 caatacccaa attttgaaac tagaggaaac agattcatgg aagctgtgaa ccgattaaaa
    30601 tagaaaaagt agaagcgagc gataaaaaat gaaaattttg gttacaggcg gaggaacagg
    30661 gggacatatt tatccagctc ttgcctttgt caattatgta aagactcaag aaccgaatgc
    30721 tgaattcatg tatgtgggag caaaacgagg tctagaaaac aaaatcgtcc ctgatacagg
    30781 aatgcctttc cataccttgg agattcaagg atttaaacga aaaatttcca tgcataacat
    30841 caagacggtc cgattgtttc ttaagagtat ccgtcaagca aaaaaaatcc tcagtgattt
    30901 tcaaccagat atcgttatcg gtactggagg atatgtttcc ggagcggtag tgtacgctgc
    30961 agccaaaatg ggtattccaa cagttatcca tgaacaaaac agtgtaccag gtgttaccaa
    31021 taaattttta agccgatatg tggatcggat cgctctttca tttaaagatg cggcttcctt
    31081 ttttcctgaa aacaaagcag ttcttgtagg taatcctcga gcacaagaag tagcagatac
    31141 taaaaaatca gatattttga aaacatttga tttggatcca gaaaagaaaa cagtcttgat
    31201 ttttggcggt agtcaaggag cacttaaaat caatcaagca gtatcagttt ttttaagtga
    31261 atatattcct tcttcttacc aagttttgta tgcttcagga gatagatatt atgaagagat
    31321 cctaaataaa ataaatcctg aaatacaaaa tattagtgtc cagccttata tcaaaaatat
    31381 ggcagaggtc atggcttcca gtgatctgct ggtggggcgt gctggtgcaa cttcgatcgc
    31441 agaattcaca gctctggggt taccggcagt gttggttccc agcccatatg ttacgaatga
    31501 tcatcagact aaaaatgcaa tgagtcttgt tcacgcagga gcagcaaaaa tgattgcaga
    31561 taatgaacta acaggtgaat cactaagtca gacagtcaac gaaatcatgg gtgatgaaga
    31621 attgcaaaag caaatgtgcc gagcttctaa agaacaagga atccctgatg cttcaaaacg
    31681 tctttatgac ttagtaaaac aaataatcaa aaaataaagg cggtggtctt agtgagtaag
    31741 aaaaaagaac caaaaaaatt gtctcatccg tcttctgatg aaaacaattt gactccttgg
    31801 caaaaggcta accgaaaata tctggcagaa caagagatga aaaaccaaga gccaaaaaaa
    31861 gaagacataa cattagatac acccctttca gattctgaag aagaaaaaac ggttcaagag
    31921 gaacagcaag aagaaagcca gtccgagaaa aaaatcgaat tttttgaaga gatcaagcaa
    31981 aagaaaaacg gtggaccata taacggttct tttttgaacc ggctgcccaa tttaaaaagt
    32041 caacggcaca aagtactatt tcgtcggtta gctttgatca tcactgtatt gggtataccg
    32101 ctgatttttt tgatctatta tgtttctcct tatagcaaat tacaagcagt tgcagtatca
    32161 ggcaataaga cagtcaacag ccaagaaatc atctctgata caaaattgtc attaggagaa
    32221 aatgtttggg gacagtattt tcatcgatct acttatatcg atcgccttaa aaaagcacag
    32281 ccaagaatcg aaacagcaaa tatacatttc aaagggatga atgaattcga tttggatgta
    32341 acagaatata aagagattgc tttgatcgca aaaaacaatc aatactaccc agtgattgaa
    32401 aatggaacag tattagatga aaaagtagca aatcctacaa aaaatctccc tatcttggaa
    32461 gagttcaaag actctgcaaa aatcaaagaa ttaacaaagc aatacaatca gttatcatca
    32521 gaattacaaa aagcaatttc tgaaatcaag tacacgccaa gagcaagcaa taaaaatctg
    32581 atccaattga atatgaatga cggcaatcaa gttattgtaa atatcaataa tcttgcgaac
    32641 caaatgaaat attattcgca agtagccaaa gatatggatg aaaaaggtgt gatcgacatg
    32701 gaagttggta tcttctcgca tccttacggt agcactcaag aaacggaaga atcgggccaa
    32761 tcggaagaaa caactgaaag tgaagaaaat gcggatcaaa caaacgaatc aagtgaagag
    32821 gcagaaacgc aaaaagcaca aacacctgga tcgtctagca caaacggatc atctgattca
    32881 aatgattcaa gtgaaacaag tcaaatagaa gataatccaa gtacaactcc gtcaagttta
    32941 aatgaaaatt gataaaacaa ctttaaaaag taagaatgta ctttctaaaa accagtaatt
    33001 tatggtagaa ttggaagcag tgaatatatc tattaataag tatattttaa aacggacgct
    33061 agttgttcgc ttgtatatgg aatagtaagt aggagggaat cccattcatg gcaaaaacag
    33121 gaatgtatgt aggccttgat attggaacga catctgttaa agttgtcgtg gctgaatata
    33181 tcgacagcca aatgaatatt attggagtag gaaacgcaaa atcagaaggg attaaccgag
    33241 gcattatcgt tgatatcgat aaaacggtcc aagcgataca acgagcagta cgacaagcag
    33301 aagaaaaagc aggtatccag atcaaaggcg taagtgtcgg tttgccagca aacatgcttg
    33361 aagtagaaaa ttgccaggga atgattgcag ttaacggaga ttcaaaggaa atcaccgatg
    33421 aagatgtcag aaatgtggct tcagctgccc ttgttcgttc aatacctcct gaacgtcaaa
    33481 tcgtgtcaat attgccacaa gattttactg tcgatgggtt cgagggaatc aaagatcctc
    33541 gtggaatgat cggcgtaaga ttagaaatgt acggtctgtt gtttacagga cctaaaacga
    33601 tcatccataa catacgtaaa tgcgtggaaa atgccggatt aatcgtcaac gaaatggtga
    33661 ttactccttt agcgctgact gaatcaattc tttctgacgg agaaaaagat tttggtacga
    33721 tcgtcattga tatgggtggc ggccaaacaa caacggctgt tatgcatgat aagcaattga
    33781 agtttaccaa tcttgaccaa gaaggtggcg aatttgtcac taaagatatc tctattgtat
    33841 taaatacgtc atttaacaat gcagaagcgt tgaaaataaa ctacggggat gcatacccag
    33901 aacggacatc accagatgaa gaatttccag tagatgtgat tggtcagtca gaaccagtga
    33961 aagtcgatga acgctacttg tcagaaatta tttcagctcg aatggaacag attttcaaca
    34021 aagcaaaaga tgcattggac caaatcgaag cattagagtt gccaggtggc gtcgtattga
    34081 caggcggagc tgctagtctt cctggtgttg tggacttggc acaagaaatc ttcggcgtaa
    34141 atgtaaaatt gtatgttcca aatcacatgg ggctgagaaa cccggtattt acaaatgtaa
    34201 taagtatcgt ggattattca gcaaacttga gtgaagtgta tcaattggca aaaattgctg
    34261 ttacgggtga aacctcagca gctcgtcaaa tggtagttga acaagaaaca acaaacacat
    34321 atgagagcta tgaagcacct gaagaaacaa tctacgatga gccggaacca aaagagtccg
    34381 gagaaaatgt aaaaaataaa atcaagggtt tctttacaaa tatttttgat taattcatcc
    34441 tgggaggaat aagatatgga attctcaatt gataataata tcaatgacgg cgcagtaatc
    34501 aaagtcatcg gagtcggtgg tggcggtgga aatgctgtaa accgaatgat tgaagaaaac
    34561 gttaaaggtg ttgaattcat tacagccaac acagacgtgc aagcattaaa aaattcaaaa
    34621 gctgaaacag ttatccaatt aggccccaaa tatactcgtg gtttaggtgc tggttctcag
    34681 ccagaagttg gacaaaaagc ggcagaagaa agtgaacaat ccttacgcga agctttagac
    34741 ggtgcagata tgattttcat cactgcagga atgggtggag gaaccggtac aggtgctgca
    34801 ccaatcgttg caggtatcgc gaaagaattg ggcgcattga ctgttggtgt cgtgacacgt
    34861 ccattcactt ttgaaggacc gaaacgtggc cgttttgctg cagaaggtat cgctcgtttg
    34921 aaagaaaatg tggatacatt attaattatc tcaaataacc gtttattaga agttgtagat
    34981 aaaaagacac cgatgcttga agctttccgt gaagcagata atgttttacg acaaggtgta
    35041 caaggtatct ctgatttgat cacagcgcca ggttacgtta accttgactt tgctgacgtg
    35101 aaaacagtga tggaaaacca aggtacagca ttgatgggga tcggtgtggc tagtggagaa
    35161 gatcgcgtag tagaagcaac gaaaaaagca atctcctctc cattgcttga aacatctatt
    35221 gatggagctg aacaagtact tcttaacatc acaggcggtt tggatatgac attgttcgaa
    35281 gcacaagatg cttcagatat cgtagcaaat gcagcaactg gcgatgtaaa tatcattcta
    35341 ggtacatcta tcaatgaaga aatgggcgat gaaatccgtg tgacggtcat cgcaacaggt
    35401 atcgatgaat caaaaaagga acgcaagtct agccgaccag ctagacaagc acaaatgcaa
    35461 tcacctgctc aaaagactgt attagatatg gatcaagcta aaccaacttc ttcagaagaa
    35521 gaaaacagtt ttggcgattg ggatatcaga cgtgaacaaa atgttcgtcc tagagttgat
    35581 gattccaatt ttgacaatat cgaaaaaaaa gaatttgata cattcaaccg cgaagaaaca
    35641 aaatctcatg gcgatgatga attaaatacg ccaccgtttt tccgtcgtaa aaaataaagg
    35701 agaggaagag cacatgattg ctgacaactt gcagaaaatc aagcaagaaa ttcaggattc
    35761 gtgtgctctt gttcatcgtg atccgaaaga agttacatta gtagctgtaa caaaatctgt
    35821 tgaatccgct caagctgaag aattggttag agctggcgtt atgaattgtg cagaaaaccg
    35881 agtagataaa ttattagaaa agaaacaaga tttatttgat tttcctgaaa taaagtggca
    35941 tttaattggt aatttacagc gcagaaaggt aaaattgatt gtaaatgaaa ttgattactt
    36001 tcatgcgttg gacagtttaa gattagcaga agaaatccag aagcggttag agaaagaatt
    36061 atcctgtttt attgaagtaa atgtttctgg tgaggaatca aaacatggaa tttcaccaga
    36121 tgagctttta ccgtttgtgg aatctttagc tgcctgcaca aaaatcaagg ttgttgggtt
    36181 gatgactatg gcgcctaaag aagcttctga tgagcaaatc cataaagtgt ttggtacgct
    36241 gaaagaattg cgtgatcttg ttcaagcaaa aggttatgcc catgcaccat gtagtgaatt
    36301 aagtatgggg atgagtcaag actttcaaat tgcgattgaa gaaggtgcaa catttatccg
    36361 tgtaggaaca gcgctattca aagacgaata aaggaggaac aaaaatgccg cttttaaaca
    36421 gaggagcaat tgctaatttt ttcggattag ctgatgagga agagtacgaa taccaagatt
    36481 atcctgaaac aacaaaaaag gctgtacagc caactaaaaa aaatacaaca cagaatcaat
    36541 caactcattc tgtatctgct gcgaatactc atgaaaaaca aaaaacggcg acttcacgtc
    36601 ctgtaacgca tgaaagtatt cagccggctg aaaagaaaaa agtaattgaa ctacatcaat
    36661 cacctacaaa aactgtagcg aaagaaagaa cagcttcaag aacctctaca caacctaaag
    36721 caaatcttgc tggaaaaatc actgtaatgg aaccaagagc ttattctgaa gctatggcga
    36781 ttgctaaacg aatcattgct ggagaagcaa ccttagtgaa ttttcattta gtagaagaaa
    36841 aacaagcgag aagaatcgtt gattttctaa caggaacagt ttatgcacta gatggagata
    36901 tcaaacgtgt tggagatgag atttttcttt gtacaccgac tgggatggag attgacagtt
    36961 caaccgctca atcttttgca gatgcgaatt tgtttgatct gtaagaggag gaactacgat
    37021 ttatactatt gcactattac ttcatttact tcaacgggca gcctatttct atagcattat
    37081 cttgatggta tatgcattgc tctcttggtt tccaggcggg tatcaatcag ggctaggacg
    37141 attattagct aagatctgcg aaccgtatat cagtttgttc gatcgtctgc ctttgaggat
    37201 tggacccttg gatttcacaa ttttaggggc aatcctgatc ttgaacttag ccgtacaagg
    37261 tttagcacag atcgttatat ggataactgt ccatttagtt tattgattga agcaacaaaa
    37321 tctttattct cagaatagat aggaggggaa aattttggat gcaaatgtat accagcactt
    37381 tcgggcagat gaacgtacat ttatcgattc ggtaggcgat tggattgaac aggttcaagg
    37441 acaatatgca ccctatctga ccgaatttct tgacccaaga caatcctata ttttagaaac
    37501 actgattcga caaagcagtg atttgcgttt catgttttat ggcggatatg aacaggcaga
    37561 aagaaaaaga tgtttgattt tccccgatta ttacgaacct gtagaggaag attttgaaac
    37621 agcattgtat gaaatccatt atcctagcaa atttgccagc ctaagtcatg ggaaaatcct
    37681 tggaacttta gtgggaacgg ggatcaaacg ctcttacttt ggcgatatca tctcggacgg
    37741 tgatcgctgg caagtattta ttgcaaatga ggtggatcat tttgttgctg cacaagtaac
    37801 aaagattgga aaggttgctg tacgcttgga accaatcaaa tacacagaaa tgattatgcc
    37861 gaaagatagc tggacacaag aaagaggaac agttagttcc atgcgactag acagtgtgat
    37921 ctctgagatt tataatattt ccagacagcg gtccaagcag ctgatcgaag ctggtaaagt
    37981 aaaagtgaac tggacagaaa atacaagacc tgattttgta cttgaactat tagatattat
    38041 ttcagtcaga ggttatggca gagtccagat ccaagaactt gaggggaaga cgaaaaaaga
    38101 aaagtacagg gtactgttcg gtgtattacg taaataaaat aaaaaggtgg tttactcatg
    38161 gctttaactc cattagatat ccaaaataag acattcccga caaaaatgcg gggatataat
    38221 caagacgaag tagatgattt cttagattta gttgttcgtg actatgagga attgactcaa
    38281 agaaatcgag aattagaaaa agcagtcaaa cattctgaag aaaaacttga gtacttcaat
    38341 gaattaaaag atgctttgaa ccaatcaatc atcgtagctc aagatacggc tgataaagta
    38401 aaaacaagtg caagcaagga atccgaagtg atcgttactt ctgcacaaaa caaagcagat
    38461 gagttagttg cgaacgctga aaaacgtgca catcaattaa caactgatgc agaagaaaaa
    38521 gcaagaaaaa tcttgacaga tgcaacagaa aaagcaagac agcttgcgac agaaactgaa
    38581 gatcttaaga aaaaaacacg agtattccat caacgtatca gcttgatgct tgagtctcaa
    38641 ttagaacaag tgaaaagtcc ggaatgggat gaaattttac aaccattctc cagctacgtg
    38701 actgacagcc atgaagtgat caaagaagtt ttagcaaaac agcttgacaa tgaaaatgat
    38761 actgatgtaa actcagacac aggagtcata gaagcaccta tgacggcatt tgatacacag
    38821 gctattgata tctcgatcgt gcctggtgaa aatgaaaacg agtagaacag aaaagcactt
    38881 tgttattctt aaagcgagcc gatgagagtg gaagttcggt aagacactgc atggtgaaag
    38941 atcctctact tgagacctgc ttttccgaaa ttagagtaag aagcagcggc tgacctcgtt
    39001 attggtcaca gagagtttgc actagataga tgcaactaaa tttgggtggt accacggcat
    39061 gttcgtccct tgcggctgaa catgcttttt tgtgtccgga aaacaaataa aggagtgtat
    39121 ggttgaatga aaatgaaaga aaccttgcaa ttaggaaaaa caaaattccc tatgcgtggt
    39181 aatttgccaa accgagaagg cgagtggcaa aaagaatggg aagaaaatcg aatctaccaa
    39241 aaaagacaag agttgaacga aggaaaacct agctttgttc ttcatgatgg acctccatat
    39301 gccaatggta atatccattt aggacattca ttgaataaaa tcagtaaaga cattatcatt
    39361 cgttcaaaat caatgtctgg ctatcgtgca ccatatgttc caggatggga tacacatgga
    39421 ttgccgattg aacaagtatt ggctaataaa ggcgtgaaac gtaaagaaat gtccatggca
    39481 gaatacttgg aaaaatgtcg tgaatatgca ctttcccaag tagataagca acgagcagat
    39541 ttcaaacgcc taggagttgc aggagattgg gaacatcctt atgttacatt agatccttct
    39601 tatgaagctg cacaagtacg cgtatttggt aaaatggctg aaaaaggcta tatttacaaa
    39661 ggattgaaac caatttactg gtctccgtca agtgagtctt ctttagcgga agctgaaatt
    39721 gaatataaag atgtaaaatc tccatctatt tttgtagctt tcaatgtcaa agacggcaaa
    39781 gatattcttg atgaagatac agcttttgtc atttggacga caacaccatg gacattgcca
    39841 gcaaaccaag gtattgctgt aaatccaaaa tttacgtatg tattggtgga agccgatggc
    39901 cgtaagtttg tcgtagcgaa agatttgcta gaaacagtcc aatcagaaat cggttgggaa
    39961 aatgtaacag ttctaaaaga atttgctgga caagaaatgg aatatatgac tgcaagtcat
    40021 ccattctatg aacgtgaatc acttgtgatt ttaggcgatc atgttacttt agacgctggt
    40081 actggattag tccatactgc tccaggacat ggggaagatg actatatcgc tggtagcaaa
    40141 tataaactat ctgtagtgtc acctgtagac tcaaaaggat tgtttacaga agaagcacct
    40201 ggatttgaag gtatctttta tgacaaagca aatcctatga tcactgatct attaaaagaa
    40261 aaaggagctt tgctgaagct agatttcttt actcatagtt atcctcacga ttggcggacg
    40321 aaaaagcctg tgatctatcg ggcaactcca caatggtttg catctatcga taaattccgt
    40381 cagaatattt tggatgaagt agaaaaagta gagtgggtta tcccatgggg taaaacacgt
    40441 ttgtataata tgatccgtga ccgcggcgat tgggtaattt cccgtcagcg tgcatgggga
    40501 gtaccacttc cgattttcta tgctgaaaat ggagaagcaa tcatcacacc agaaacaatc
    40561 gaacatgtgg caaaattatt tgctgaacat ggatcaatta tctggtttga aaaagaagcg
    40621 aaagaacttc ttcctgaagg atttacccac ccaggttctc ctaatggcga atttacaaaa
    40681 gaaaaagata tcatggatgt ctggtttgat tctggttctt ctcatgaagc tgttttacgt
    40741 cagcgaccag aattatcatt cccagcagat atgtatttag aaggatcaga ccaatatcgt
    40801 ggctggttca actcaagtat cacgactagt gtggctatca atggtgtagc accatacaaa
    40861 tcagttattt ctcaaggatt tgctttagat ggtgaaggcc gtaagatgag taaatcttta
    40921 ggaaacatca ttgcaccaga aaaagtgatc aaacaatttg gtgcagatat ccttcgctta
    40981 tgggtatcta gtgtagatta tgaagcagat gttcgtgttt ctatggatat cttaagtcaa
    41041 gtggcagaag tatatcgtaa aatcagaaat acgatgcgtt tcttattagc gaatacagaa
    41101 gatttcgatc ctaaaaaaga tactgtttcg tacaatgatt tacgttctgt agacaaatat
    41161 atgaccgttc gattgaacca agtaatcaaa gaaatccgag aagaagggta cgacaaatat
    41221 aacttcatgc atatttaccg tacagttatg aacttcttga cggttgatct atcgtcattc
    41281 tacctggatt ttgctaaaga tgtagtttat attgaagcag aagatgatta tcaacgtcgt
    41341 tgcatgcaaa ctgttttcta tcaaacagct gtagctttga caaaattatt gactcctatc
    41401 attccgcata cagcagaaga aatctggtca ttcctgaaag aagaagagga gtacgttcag
    41461 ttaagcgagt ttcctggtta tgaagagttt gcaaaccaag aagaattgat ggatacctgg
    41521 actgctttca tggacttccg tgataatgtc ttaaaagcat tggaagaagc aagaaacagc
    41581 aaattgattg gtaaatcttt ggaagcaaaa ctaactgttt atccaaaaca acaagttcgt
    41641 gaaatgttaa aagctttgga tgcagatatt gcacaattat tgatcgtttc accagactac
    41701 tttgaagtta tggcagcaga tgaacaagtg ccagataacg ctatggtatt tgatgatgtt
    41761 gctattacag tggaaaaagc agacggggaa acttgcgatc gctgtcgcca agttcgtaaa
    41821 gatgtaggag tagatgaaaa acttcctcat ctatgcggtc gttgtgcaaa aatcgttgaa
    41881 gacttctatc ctgaagctgt agctgaagga ttcgaagaaa agtaaaaggg aaaagggggt
    41941 gtgacatgtt ctttgtcaca cccccttttg ttatcaatcg tttaattttc cacgttcacg
    42001 ataccagtta tctggttgcc agatccattc gaatccgtct ttttcaagta gttcgaaagc
    42061 tgctttaggt cccattgatc cagctttgta gttgggaaaa tccggttgtg tttggtccca
    42121 agcttttcga atgacatcca cgatatgcca agattgagcg acttcttccc aatgagagaa
    42181 gttcgttcca tcaccattca acgcatctaa tagtaatttt tcgtaagctt ctgggctatt
    42241 ttcaaccatt tctgcactat ttctaaattc caatttgatt ggttccgttt caaatccttg
    42301 ccctacttct ttcccattca aggataaaga aaatccttca gttggctgaa tatagatcgt
    42361 tagaacatta ggtggaagtg tcgtatcgtc acatggttca tcgacagatg ttttgaatac
    42421 attaacagga acttgtttga atacgatgtt gatccgagtc cctttttcag tcaaacgttt
    42481 gcctgtacgt acatagaaag gaacacctga ccaacggaag ttatcgatag tgaatttgcc
    42541 tgcgacgaat gtttcagtct ctgattgagc atccactttg tcttcttcgc gatagccctt
    42601 gaattgtaca ccatccaatt caccttgagc atattgcccg cgaacgaagt tttctaacac
    42661 ttcttcttcg ttataaatgc ggatagcttt caatgctttg atcttttctg tacggatttc
    42721 tttttcggaa aatgctactg gcggttccat tgcaagtaat gctacaacct gtaaaatatg
    42781 attttgcacc atgtctttaa gtgcgccact atgatcgtag tagcccccgc gatcttctac
    42841 accgaggttt tcgccaaaag taatttggat attgtcgata tagcgattgt tccattgagc
    42901 ctcaaaaata ttattggcaa aacggatagc tgagatgttt tggatcattt cttttcctaa
    42961 ataatgatca atcctgaaaa tatcttgttc tgggaatact tctctgattt gctggttcaa
    43021 ttcaaaagca gactggtaat cagaaccgaa cggtttttct atgatcaaac gattgaagcc
    43081 attttttgtc ataatatttt gtgatttcaa atgagaaacg atcgttccaa aaaattgagg
    43141 tgccatagcc aaataataga cacggttacc gtttaattga tagtgtccat ctaatcggtc
    43201 agacaattct tttaatgtct gataatgcgt tgaatcattt acatcatggg attgatagta
    43261 aaaatggctt gcaaaggtag ttgcttcttc atctgaagga ttcaatgcac ggattgtttc
    43321 tttgacaact tcacggtagt gctcatcgct ccagggacgg cgagcggttc cgattacagc
    43381 aaaatgctcg cctaaatttc cttttctgta aaggcggaac aaagacgggt acaatttacg
    43441 ctgggctagg tcacctgtac cgccgaatat cgtaaacaat acattttttt cattagacat
    43501 gtggatacac tcctttatat tttatcgaca aaaatcgtgc tggccgcctt aaagctgacg
    43561 gggattttct ttctatcatt ttggatagtg atcggtcctt cataagctgc gatgtcaacg
    43621 atctcatatt cttcgttgat attcaggtca atagtgacta agtaatccaa caattctttt
    43681 tcatcaagca ctcgtgcgat acgcactttt gtaccgacag gataggactc caatgtttgt
    43741 cggcgttctt catggacaac ttcctctttt ttaggaatcg atcctccatg aggacaatgt
    43801 tcaggatgat tcaaatagtt ttctaaatga tctgcaagat gttccgaagt cacatgttcc
    43861 aatacttcag catcatcatg gacttcattc cagctatagt ctaaatgttc aactaaaaaa
    43921 acttcccata agcgatgttt gcgaatcaga ctgctagctt tttgcagtcc gcttgcagtc
    43981 aattgtacac cttgataagg ggaatgttct acaagctctt ctttcacaag tttagaaatc
    44041 atttcactga cagatgcagc tgagacatct agactagata cgatttgttt attgttgact
    44101 ttcgttgtat ctccacctag ttctaaaatg attttcaaat aatcttctcg atttggtgtc
    44161 atattcgatc atcctttcat ggtaaaaaac catcattagt aagtgtaaca aaaaaaacgt
    44221 taattgacta tttttctaaa taaaaaacaa aggattttta gacgttttta caacaaaaaa
    44281 cgaaagatga tagagaagac tcattctttc gtttataaag gatatatata tacattggga
    44341 gggatctatt tttcagataa caacttatat gaagtgtctg cagattcgtc ataaagagtt
    44401 tgttcgggat aagaaaagat aagataaaaa ccatactgat cacgtacgac taaacgatat
    44461 tcatctattt ctaccacaga tgtgccaaga gactgaccgt tgatcaaaaa cgtgtaatca
    44521 gatgtaatct gtaattgttg tttttgctgg ctatcactaa agaaccatgt accttgatac
    44581 tttgagagtc cttctgactt ttccgcttgt tttttctttt tcaagtaaat aaaacttgag
    44641 agaacggtaa tgagtgaacc aagaaaatag atcatttttt ttatcaccaa atcactcctt
    44701 tatgagaaaa taatcgcata gacatattct ttcttcttta tttcattgta ccgaataaaa
    44761 gctaaaattt ctctaagact ttcgactggt ttttttactg tttttctaca aattatgata
    44821 ggatacatag taggaagaaa ggtgacataa tttgaagtat cagcaagtgg aaatagcttt
    44881 ttttattgac cgcccgaatc ggtttatagc acattgtatg aaaaaagacg gagaaataat
    44941 tattgcgcat gtcaaaaata caggtagagg aaaagaggtc ctgataccag gcgctgaagt
    45001 ggctattgta tttgctcctg gacctaaacg gaaaaccgcc tatgatctga ttgctgtaaa
    45061 aaagaagggt gactggttta atatcgatag tcaattgcct aatcggctgg caattgatgg
    45121 tattttagac ggcacgatcc ttttaccagg attaactggt ccatttacgg tatacaagcg
    45181 tgaagtaact tatcttcact ccaaatttga tatttattta gagacggtgg tcggacaaaa
    45241 agcatttgtt gaagtcaaag gtatgacact tgaaaataaa gcgattggtg cttttcctga
    45301 tgcgcccaca ctcagaggat tgaaacatat aggagaatta acgtatgccg cacaagatgg
    45361 ttatgccgct tatgtcttgt ttattgctca gttcgaacat cttcatctgg caacgatcca
    45421 tgaagaaatg caaccagcac tagctgatat ggttcgacat gcgcagcaaa gcggagttca
    45481 aatcctcgcc tataattgtc aagtatcggc agatgaggta actgtcaagc aggccattcc
    45541 atttgatgta gatatccctt ttgaagatcc tatacaatag aaagaaggta aaataatgat
    45601 caacttaggt attatcggaa caaattggat tactcatcaa tttgtccaag cagctttaag
    45661 cacaaaaaaa tatgaattga cagcggtata ttctagacat ttggaaaaag cacaggaatt
    45721 tggttctcat tatgaagggg atatcgaatt cgcaacagat ttgaagactt ttttcaatat
    45781 tactcatatg gacaccattt atattgcttc tccgaatagt cttcacttta gtcaagctaa
    45841 gcaagcaatt ttggcaggaa aaaatgtgat agttgaaaag ccagcttttt cgacgccaaa
    45901 agaaatggat gaaatcatcc atttggcaaa tgaacacaat gtgtattttt ttgaagcagc
    45961 aagaaatatc catgaatcag gttttaaaaa gattgccgat tacttaccat taaaagacga
    46021 gataatcggt gcaaatttca gctatatgaa atattcttcg cgctatgacc aagtattaga
    46081 tggagaagaa ccgaatatct tctcgactca tttctcaggt ggtgcattga tggatttagg
    46141 tgtttacctt gtatatgcag ctgttgcttg gtttggaatg ccaaaagaag tccattattt
    46201 cccaagaaag atttctactg gtgtagatgg tcttggatgg gggatcttac gttatgagaa
    46261 ttttgatgta tcgatccagc caggtaaaat cggtgattct tatttaccgt cagagattta
    46321 ttttgactca ggaacgctag tgatgaatgg agtcaatgca attgaacaag cacaatttca
    46381 tgatcgcgcg cataaagaaa tcattgattt ggcggtaaca gctgcagaaa atccaatggc
    46441 agatgaagca gaggattttg ccaatgtcat ggctcatccg aaagatccag cttggggatt
    46501 gttgtatgaa gagtggatcg agctagctag aaatgtgaac caagtgattt atgatttaag
    46561 aaaaaatggc ggcatcaaat tcgatgcaga taatgaggaa gatttttaat gaacgaattt
    46621 gtacaagtat ttcccgaaaa ttggcagaaa aaatatgagg ataaagggtt tatctcacct
    46681 tctcttattc aacgagcagt ttttcagcca ctaagtaata aacaaagtat agtagcggtt
    46741 tctcctacag gttcaggtaa aacactggct tatctttggc ctctactact aaacgtggaa
    46801 ccaggtgaag caagtgcgtt ggtcattttt gcttcttctc aagaactagc tattcaagta
    46861 gcagatgttg cacgtgaatg gggaaaagac aaagagttga aagtccaatc tttggtgggc
    46921 ggagccaacg tcaaacgtca gattgagggt ttgaaggaaa aaccagaaat cttgattggt
    46981 acaccaggaa gaatattgga attgatgaaa gcaaaaaaaa taaaagcaca tcaagtgaaa
    47041 acaatggtat ttgatgaagc agaccagcta tttgacgctg ggaacagcca aatcattgat
    47101 caaattcttc atcaagctcc aacagagtat cagctagctt ttttttcagc gacagcagat
    47161 cgttcccttg aaagcattga aaaaatcact gggaaaacgt ttgagacaat tgacgtgaca
    47221 gcagaggacg attctcgaaa aggactgaga cattactttt tacgtgtacc gatacgaaag
    47281 aaagatgact acttgagacg tctcacgcat atagaagatt ttcatggtct gattttcttt
    47341 aatcaactga ctgaattagg cgtaatggaa gaaaaattgc tgtatagagg tgtaccggta
    47401 ggaagtctgg cctctgatca gaacaagcta ctccgaaaag cagcgctaga acaattcaaa
    47461 aagaaaatgg tcagagcact tttgaccaca gatgtggcgg ctagaggttt agatattaca
    47521 ggtcttcctt atgtagtgaa tgcagaagtt ccgttaagtg aagaagctta tttgcatcgt
    47581 tcgggaagaa ctggtcgaat gggtaatgaa ggaatagtaa ttacactcgt acaagataat
    47641 aatctaagag atttaaaacg gcttttacgt ccatcaaata taacacttga agagattttt
    47701 ttatacggag gtaagctgca aactgagcag ccaaccaaag aagatgcagc tcctgcagta
    47761 aataaaaagt atgcgaaaac tttttctgga aaggagcaga acccagaata taacaaagaa
    47821 aaaataagaa aaaaagttaa aaataaatca aaaaaagaga gaaataaagg agcaaggcgc
    47881 aaataagcgc ctttttatgc tatgattatg aaaacgggaa caaaattggt tgccagagga
    47941 ggggttatgt gaaagaacgt caagaagaga tcttgcagct attgacagaa aaccaacaag
    48001 gattgacagc aagtgatgta gctgagcgct taactatcga tcgaagcaat gcaagccgat
    48061 atttaagtga actatacaag gcccatcata ttgtaaaaac cgctggccga ccagtcgttt
    48121 actctttgcc aacggaaaag tcaaaatctg atgaagtgca tgtcgactct tctacgcaag
    48181 tgacttttga aacactcgtt ggagaaaatg attctttaaa agtcagcata cagcaggcaa
    48241 aagctgcgat tctttatcca ccgcgggggt tgcatacgat tatttttgga gaaactggta
    48301 cagggaagtc gatgtttgct gaatgtatgt atcattttgc aatcgattca gagatgcttt
    48361 ctgcagatgc gccatttgtt tctttcaatt gtgcggatta tgctcaaaat ccgcaactgc
    48421 tctttggaca catctttggg atcaaaaaag gggcttacac aggagcagct caagatagtc
    48481 ccggtctaat agccaaagca gacggcggta tattgttttt agatgagatc caccgtttac
    48541 cgccggaagg gcaggaaatg ctgtttactt tcattgataa aggtatttac cgtccgcttg
    48601 gagaaagtgc acaagtacat gaagcatctg ttcagatcat tggggcgacg actgaatctt
    48661 cagaaagttt cttatctacg ttcaatcgac ggattcctat ggcaatcacc ttgccgaatc
    48721 ttgcttcacg ttcactggat gagcgttatg aaatcatctc tctttttatc aaacaagaag
    48781 cgaatcgttt gaatcaacgt atcgatatcg aaaaagaagc gattcttgcc ttcatgcttt
    48841 atgatgcgga aggaaatatc ggtcaagtga aacgagactt gaaactagtc tgtgcaaaat
    48901 cttttttaca ttaccgtact catcatgaga aaaagctaat cattcgaaaa gaagaacttt
    48961 cccttcaagt acaaaaagga ttactgaaaa tcaaggaaat accggaacgt ctcgaccgat
    49021 tcatggacag caaaagtcag tatttgacat ttgaaccagg atttgctgat gttgtctggt
    49081 cacaagatcc agaacgaaac atgcaggtct ataacgatat tgaggaaaaa gtcacttctc
    49141 tttccgaaac cggtgtggag aacattgatc tagagaccct catttcaaaa gatgtggatg
    49201 cttatttcca gacatatgtc aaagaattga ctaaaagttc gattcccaaa gatcttttgc
    49261 cagatgaaat ctggcggtta accaatcaat tgtatgatgt ggctgaaaaa cggttagacc
    49321 gtatttataa tgaaaaagct cgttttgctt ttgctttgca tctgcaaagt acattggatc
    49381 gtgtgagaga aggtcatatg atcgtccacc cagatttgaa taacgtacgg aaaaatttta
    49441 agagggaatt ccaggtagca atcgatcttt caacgatcat tgaagaagaa gaacaagttg
    49501 agattccttt tgatgaaatc ggctttatta gtatgtttct ttctatcaat ctaggagaaa
    49561 gtgagcctgt taaagaaaac aaagtagatg ttctcatatt gatgcacggc agacagactg
    49621 cttcaagcat gttggagatg gcacaagaac tattaggaac aaaagtaggg aaagctttta
    49681 atatgccact ggttatggaa gttcaaacaa tgtatgaaga agtgttacaa tatgtcaaaa
    49741 actatcaaga gcatttgacg aatggcctac tcttgttaac agatatggga tcattaaaca
    49801 ccttcagtga tttgatttat gaagagactg gcattcggac gaaagctatc acgatgacaa
    49861 gtaccatgat tgtgatagaa gcgatacgga tggcaagtgt tggaagaagc ttagaagata
    49921 tttatcaaaa tatccagctt tcctttgaat cgatcgtacg tgatcagttc aaagcggaaa
    49981 agtctgaaac tttacagcgt aaaaaagccg taatcgtgac atgctttact ggtgaaggag
    50041 tagctgccaa attatatcag cgtattatgc cagtgattaa tcaaaataag gtagaactga
    50101 tccagatgca gtttttagaa cgtgaatcat tcaaaaaaca catcgatgcc ttattggaag
    50161 aatatgagat caaagcaatt gcgggaacag ttgatgttga ttatcaaaat atcccatttt
    50221 tttcagccta tgacgttttt gatgatgaaa aactaaatat tttaaagcgt atagctgatg
    50281 atgagatccc gacagctaaa attgtcgatt cacttgctgg atcattaagc gatgtctttt
    50341 ctattgatga actgatccgt tcattgcaaa agacagttca gcagatccag accgatttgt
    50401 cgattatcgt ggaacctgga gtagatactg gaatcgttat ccatttagcc ttcatgttag
    50461 acggattgat caaacaagaa agccatcggc aatttgagaa actgtctatt ttctcaaaaa
    50521 tttatcgttt ggaaatggat atcgttcggg caaacttgat ggcactagaa cgaaaatatc
    50581 aggtaaaaat tccagaagat gatatcgcat ttcttacaca aatgtttctt gaaaataaaa
    50641 taaaaaaatg aacacactaa tcatactgtt tacacactaa acatctgtgt gcgcactata
    50701 aaaaagagct aaaaagcatg aaaacgctat ttcagctctt ttttttattg gcatgcttct
    50761 tgcgttatat aaaagtgtaa aaaaataaaa ggagtgttaa aaatggcaaa aaaaacaatc
    50821 atgttagttt gttcagcagg gatgagtacg agtttattgg tcacaaaaat gcaaaaagca
    50881 gcagaagaaa aagggatgga agccgatatt tttgcggtct ctgcctcaga tgcagataat
    50941 aatttagaat ctaagaatgt cgatgtatta ttattaggtc ctcaagtacg tttcatgaaa
    51001 gctcaatttg aacaaaaact tgcgcctaaa ggtattccat tagatgtcat caatatgcaa
    51061 gactatggca tgatgaacgg agaaaaagtt cttgcacaag cagaaaatct aatgaaataa
    51121 atcaagtatg cagttggggg tataacaacg tggaagatca acaaaatcta gaagccatca
    51181 tggggttaat catgtttggc ggtaatgcaa agagcgatgc aatggaagcg attgcggctg
    51241 ctaaaatcgg tgattttgag ttagcggata ggaaaatcgc tgatgcagaa gaatcactta
    51301 tacaagcgca tcatgcacaa acagacatgt tgacacaaga agcacaagga aatcatatgc
    51361 aagtgacatt attgactgtt cacagtcaag atcacttaat gacatctatt gcgtttactg
    51421 atttagcaaa agaaatcatc gaactttatc gtcgcgtata cagcgaataa atacaacttc
    51481 ttttttgaac ggaagaagaa aaatttttga acggattttt tattttgtta taaggagaac
    51541 tctctttata taaaaaactt ataggaggaa gtaaaaaatg aacggactat caagttggat
    51601 ggagaaatac atcctgccgg ttgccgcaaa aatcggagcg caaaaacatt tagttgcttt
    51661 gcgtgatgct tttatcggca tactgcccgc aacaatggcg ggatcagtcg ctgtaatgat
    51721 caatgcaatt attcgtgatt taccgcaaca gtttaacagt acctatgctg acagtttagc
    51781 agcagacaaa ggaatctttg ccgtattgaa tgtcatcatc ggaatcaatg gttttgtctg
    51841 gaacggtaca ctagcaatcg ccggattgat ctttgccttt tcttgggggt ataatcttgc
    51901 ccgagcttat ggagtaaatg atctagctgg ggggatcgtt ggtttagcta ccttgattca
    51961 aggtatttcc tttggtccag cattatcagc agcattagat gtgaatgttc cttcaaatgt
    52021 cgcagatgca atcaatagtt ctgagattgg tgcaacgatt gcagatggca cactgtcagt
    52081 tggattatgg ggttggctaa aactagatca tttgaatgga aacgcttatt ttacagttat
    52141 gatcatggga gctatttcag tcattatttt ttgtaaattg atgcttgcca acattacgat
    52201 caaaatgcct gattcagtac caccagcagt atctaaagca tttgcagcca ttttaccagc
    52261 gacgatcgct ttatacgtca ttgctattat caattatatc gtaggtcgtg tagctgatgg
    52321 gcaactgatc attgatttgg tgcaaaaata tattgcagaa ccattcctag gattatctca
    52381 agggttggga gcagtattga tcgtcacagt atttgttcaa gtattctggt tctttggtat
    52441 tcatggatca aatgtattgg caccagtgct agagggaatc tggggacaag cacaactaat
    52501 caatattgat attttccaaa aaggttataa cggaaaaact ggtacaccag ccgtgttaga
    52561 tgcaatcgaa gatggaaaag cttatatgtg ggtccgcgga tcgttcgatg cttttgcatg
    52621 gtttggcgga tcaggcggaa cgatcgtctt actgatagct atcttgattt tctctaaacg
    52681 tgcagattat ctaacagtag ctaaattatc cattgggcct ggtatcttca atatcaacga
    52741 accagtgatg tttggtctgc caatcgtatt gaatgcaatc atgtttatcc cattcttgat
    52801 tgctccagta gtagcaacag caattggatg gacagctact tatcttggcc tagtcgctcc
    52861 ggtatcgcag caagtcacat gggtagtacc gccgttctta ttgtcattct tagcaacagg
    52921 agcggattgg cgggcaccga tcgtaacatt agtatgtatg gtcgtgactt tcttgatttg
    52981 ggcaccattt gtcatctctg caaacaaaat ggaccctgcc gcaggcgaac aacaataaga
    53041 tgaacgtgag gagcgggacg ctttaagttt cgttcctcat aatgaatttt cgaggaggaa
    53101 ctgaaatgat tacaattgcc agtgcaatgc aacaagatga aatcaaagaa ttgttagaag
    53161 gatacaatga agaaggaatg acttttacat ttaaagaaaa acaaggaatc aagctgttct
    53221 ttgaaacaag tgcagaagat ccggaagttg cggctaaaaa agcaaaggaa ctaatcaaag
    53281 cacagccttg gggaacagtc ctttattttc aatcaacagc tgtttagaaa atggtttaaa
    53341 aagataggat ggttgagatg ataaaaaacg gtttattttt atgtagtatt ggttctttga
    53401 tcgtgttgta tagtttgaat ccacattcgc aaaggtatga tttgtatagt atggtcacag
    53461 gtctttttct aacaggatta ggaatctatt tcttcatgaa ggggaaaaaa agagaagaag
    53521 caaaggagaa aaataactaa tggaaatcac gaaaaaatta aatattccag catcgttttt
    53581 ttacgataaa gttatggact ctgtcctttt cgatgtacgt aaagcgaccg gcaaatcagt
    53641 aactagaaaa cagctgaaaa atttggagta cgtcaaacaa ttctcgcaaa ataaccgggc
    53701 tcgcataaaa atcgaagaag tcattgaaaa ccagtcttat aagtttcgta catcgacaac
    53761 gaagaatgaa ttcgtcgttc attatcaaat caaaccattg gacgattcga cctgtgaggt
    53821 tcattacaca gaaaaaatgg agtcttatgg catgcttcaa aagttgaatg atatgctttt
    53881 aggaacaatc ttgatgtatt ttaaacggag acgtttcaaa aaaatgctag atatgatcga
    53941 agcaacgtat taaatcaatg aaacatggta ctatttagtt attgaaaaat gaagtaagct
    54001 agaaggaatt tttcatagga agatagatct ttcctgcgaa tcctcactag cttttttgta
    54061 ggaaggggca gcaaaatgac aaacaaagta agagagctag gtgtatcttc gattgaggaa
    54121 atgtttgatt tagccactta tgcatttaat ggggcagata cacctgaaag gcgagaacgt
    54181 tttcgtacac ttgcggagaa ttcttggaat tacggatttt ttgatgagga ggatcggcta
    54241 acaagtcaag tcatggcgac cccttttcca gtgaattttt acggacaaga gtacctaatg
    54301 gccggcattg gctatgttgc ttcttatcca gaagcaaggg gacaaggcgg gatcaatcag
    54361 atcatggaaa agatccttga agattgccgt gatcgtaaag taagcttatc ttatctggcg
    54421 ccattctcct atcccttcta tcggcgctat ggctatgaac aatcatttga taaaatcagt
    54481 tatcaactaa acagtcgtga tgtgccgttt atcaaaaaaa aatctggaaa aataaagcgt
    54541 atggattttg aagatgcaaa agctgcaatc agaagaattt atgaacagat gccggaaaac
    54601 caacgagctg gtctgaagcg cgaagactgg tggtatacgt ataaattcaa gcaaaaaggc
    54661 aattaccagt ttgctttgca ttttgatgat aatgaggaag caacgggata tatcgtttat
    54721 cagcttgcaa cgtcttcctt tattattgcg gagtggggat ttttggatca tgatgctttt
    54781 tgcagccttg ttcgcttcgt aagttctcac aatggtgctt ttgaaacatt tgaatattca
    54841 tgcggctacg gcggtaatga tcaaaattat ctattagaaa atccatttgc ttctgtaatg
    54901 atcacacctt atatgatggc gaggatcgta gatatttctt tgtttttgga aaattatcca
    54961 tttaaaaaga gacaagctgc atttgcccta caaatagaag aagatcatta tgcgaaatgg
    55021 aatgaaggaa tcgtggaagt aacaatcgaa aatggaaaaa atcatctctc aaaagtagaa
    55081 caaacaaaac tcccaatcgt tcgaggaagt atccaacata tcacgcagct attattaggg
    55141 tatcgtaaag tggaagagtt ggtattccaa gaaagaatac aggtgacaaa agaatatgaa
    55201 gaaacgttaa gctcaatctt gccacagcaa cagccaattc ttaatgacta tttttgataa
    55261 aaaacgggtg atgacaatat ttttgtcacc acccttttgt ttttcatttg gcatttgaag
    55321 gaaaatgtta aagtaaatcc tcttttttcg taaatttgaa ttttttgttt tcttttgttt
    55381 tagggttttc ttttttgtgg tcttggattg cttctttcgt aatcttttcc cgattttttt
    55441 tagatgaatc taagcgttct gcgtatactt tttcttttgg cttatcattc attggaaagg
    55501 acctccctac attatttcat tctacctcta gtttatcatc tgttaaggac tagtggaaac
    55561 gttttgccta acttataagc tgattttttt agcagataag cttgcaagta aaaaacaaac
    55621 aagctacaat tgatttattg aaattgttcc tgtagctcag taggatagag caatcgcctc
    55681 ctaagcgata ggtcgctggt tcaattccag tcaggaacgt ataaataacc cctgtatcct
    55741 ttgcggtaca ggggtttgtt ttgtatttgt gccaaaaatg tgccaaacta gttatcattt
    55801 ttatataaac tatcgaacac agtattgatt ttttctttat cctgatcttc aagctctcga
    55861 acaatatgaa gataagtaga cattgtagtt tctaaactgc tatgtcctaa acgtttcgat
    55921 acgctaagta tatttactcc ttgataaagt aaaatagatg catgtgtatg ccttagtcca
    55981 tgcaaagtaa attgcttttc aatccctaat tcttttaatt ttttgcgcaa atatttgctg
    56041 acggcgtttg atgaaactaa tccatttttt aagttgaaaa aaacaaaatt atgagggttt
    56101 ttgatttcaa aattctcgaa taattctttt tgattgattt taaacttttt taagagatca
    56161 atcgtatgtt tatcaatgga aattttccta tttgaagttt cgttttttgt ttttccccat
    56221 tcatttaatt tataattcca tgttttatta atagtgatga tttgatcttc gaaatcgata
    56281 tcttcccatg ttaatcctag aagttcagca aatcgcattc cagtggcacc agcaactaac
    56341 acgagcatag gagaagagta ctgcgcagaa aggttttctt ctacgagttt cattaaagat
    56401 ttaaattgat cataatctaa atatttatct tcttttggct tcaatgaatc ttttcctttg
    56461 attactgctt ttctagttgg atcaaacgga attaatcctt cttctacggc atctttcaac
    56521 gacgctctaa tatggttatt aaacttaata acggtagatt ttacatggtc tttagcatac
    56581 ttatttaaaa agcgttgata tcccactcta tctaaatcag aaatcaatac cgctggcata
    56641 tatttcttta tgttcattaa cgtatcttcg tattttctat aagtgatagg agatactgtt
    56701 ccttctttat aaagttgcat ccaatcctca aagtaatctg ataataatag atttttgcga
    56761 tcccctttaa ggcctttgtt caagttatat tctaattcgt tggcggcatc ttttgcttct
    56821 ccttttgtct taaatcctga ttttcttatc ttcgtgtatt ttccgtcatc ttttttgtat
    56881 gaaatttcgt attgccaact atttccacgt ttgactaatc ttgccataat tgaatcagct
    56941 ctctttctct gatacaatag gaactataaa gaagcccatt gtttgggttt tattattttt
    57001 tgcacatcct ttttgcttgc cggcgcaagg atgtgctatt tgtcgtttta atggtataat
    57061 tggatgtgta ttagatcatc caatcagagg tgtttattat gattaaagtg atttttaaaa
    57121 gaataactga acttccagag tttagattat ttgttgatta ttggtatgtc tggtttttta
    57181 ttgttgcatt gctgataggt ctagatgtca tattagaaag aaaacttaag taagagttga
    57241 gttaataaat tgaattccta aatgagatat tttgtagccc atattttgtt tgccgacatc
    57301 acctaatact atggagctaa tcgttttttc taacagacca aaatcagata atctatttaa
    57361 gctaccatct attagctctt gtgaatagcc agacatgctg atatttccag tgaaattttc
    57421 gtttccacga ctcattaagc atgctaacaa ttgaatctca atcgatgtta attgattcag
    57481 tacaagaaga taatactcct cgttatccca tgaagcgttg ttaattcgaa gtaaacaatt
    57541 cttataaaga tttttataca tatctatttt attttgtgcc tttgattttt ctacctcgtc
    57601 atttatccct tcgaatattc caatgagttg gtccttgttc tctaagttga aaacatcttc
    57661 tggaatttga tcgcgaatgt tttctaatga ttcgtttaaa tttttgtaga atttctcgat
    57721 gcgtttgaaa cgcttctcgt ttttagaccc aaaataaagt gactgcacaa cgccaccaat
    57781 ataaggaatt gctccaatac cgccttcaac tattaattca agatattctt ttttggttag
    57841 tttttcattg ctattatcca tagttacctc ctattttgtc atttccttta caagcattgt
    57901 gactcctgat ggcactcttt tgtataggtt ttattattgt gcacaatcta actcttagtg
    57961 gggcgtggtt catttatttt ttacttactt tactattaat catatcccat tgagcatagg
    58021 atttttctat gttcgatcct cttgaactaa tttctcctgt aaaagttact ttatcgccct
    58081 ttgtaaaggt atcagcattg tttaagtcct tcgcaaaaat tacatatgaa actttaggtg
    58141 ttgcagaaat atcattccaa cttttatcat cgtactcttt gtctgctata atggctattc
    58201 tagtagtcat tgattcgata acagttcctt caaaagtata tgttttacca ctaatatatt
    58261 tagtaaaagc tgtattttgc tcagtagctg gagtaataga ataataacta tctaagaaag
    58321 caccccagtc atgattattt gtctctaaaa attcttcaaa ttgagttagg cttttattgc
    58381 tgctgttaga tgtagtagtt tgagtatcag atgatgagct ttcctgtttg ttattgtcat
    58441 ttgcctcgtt tgatgaggaa agcgagaaac ctacggtagt aaaaataatc ccgataataa
    58501 ggagaatcat aggtgttttc ttatttgttt ttttgataaa tcttacaatt aacatgatta
    58561 aacctataat aaataaaaca ataccagtaa aaccaaatat accgtccatt aatagatcct
    58621 ctttctcttt tgatattgaa tattataaac caagcaattc ttttttctta aaatcgaatt
    58681 cttcttgatt aataatccct tcatctaaca actctttata ctttttaatt tcatctgcac
    58741 ttgaaatttg tgtttctttg gaattattat ctaatataga ttgttgcttc tctatatatt
    58801 tttttaaatt ttccatttca ttccaatatt tttttgtaaa cataacggtg ttttcatctt
    58861 tcgtggccgc taatatacct ccgcgacttt cgtttccgcc tagcaaggta aattgtatat
    58921 aaccattact catacctggt ttttttaatt gtacggctga tatattttta aaggggatag
    58981 ttttttctcc tttaatacct tgattaacta aatttataaa tccttttcta ataattgaaa
    59041 tagaatcatc agttacttta atcaaggttt ttcctggaga ttttattaaa atctcttttt
    59101 ccattttttc ttcctcattt ctgtgatatg attttttttg tagaagctca tatatgagga
    59161 aagagcccgt gttgcagcac gggctttttt aatgttttgc tgaaatcggt ttttttaata
    59221 attcttgatg tattcataca tattgccttg cgtaagaata ttttttctta aaatagcatt
    59281 ggcagacagc ataacaagtg tatctgagct tattctgaat agaataatac tccataaatt
    59341 tttcgagatt aaactgagat tcatcagtca g
//
LOCUS       DAZBSA010000003        59135 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000003 DAZBSA010000000
VERSION     DAZBSA010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.003"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(122..499)
                     /locus_tag="ACHGMT_000163"
     CDS             complement(122..499)
                     /locus_tag="ACHGMT_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6833942.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            755..1483
                     /gene="purC"
                     /locus_tag="ACHGMT_000164"
     CDS             755..1483
                     /gene="purC"
                     /locus_tag="ACHGMT_000164"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFN6833943.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1483..1737
                     /gene="purS"
                     /locus_tag="ACHGMT_000165"
     CDS             1483..1737
                     /gene="purS"
                     /locus_tag="ACHGMT_000165"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFN6833944.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1739..2410
                     /gene="purQ"
                     /locus_tag="ACHGMT_000166"
     CDS             1739..2410
                     /gene="purQ"
                     /locus_tag="ACHGMT_000166"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFN6833945.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2411..4633
                     /gene="purL"
                     /locus_tag="ACHGMT_000167"
     CDS             2411..4633
                     /gene="purL"
                     /locus_tag="ACHGMT_000167"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFN6833946.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4618..6057
                     /gene="purF"
                     /locus_tag="ACHGMT_000168"
     CDS             4618..6057
                     /gene="purF"
                     /locus_tag="ACHGMT_000168"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFN6833947.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6080..7132
                     /gene="purM"
                     /locus_tag="ACHGMT_000169"
     CDS             6080..7132
                     /gene="purM"
                     /locus_tag="ACHGMT_000169"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFN6833948.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7129..7707
                     /gene="purN"
                     /locus_tag="ACHGMT_000170"
     CDS             7129..7707
                     /gene="purN"
                     /locus_tag="ACHGMT_000170"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFN6833949.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7704..9239
                     /gene="purH"
                     /locus_tag="ACHGMT_000171"
     CDS             7704..9239
                     /gene="purH"
                     /locus_tag="ACHGMT_000171"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFN6833950.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9255..10529
                     /gene="purD"
                     /locus_tag="ACHGMT_000172"
     CDS             9255..10529
                     /gene="purD"
                     /locus_tag="ACHGMT_000172"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFN6833951.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10651..11565
                     /locus_tag="ACHGMT_000173"
     CDS             10651..11565
                     /locus_tag="ACHGMT_000173"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFN6833952.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11698..13263)
                     /locus_tag="ACHGMT_000174"
     CDS             complement(11698..13263)
                     /locus_tag="ACHGMT_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6833953.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13256..14707)
                     /locus_tag="ACHGMT_000175"
     CDS             complement(13256..14707)
                     /locus_tag="ACHGMT_000175"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN6833954.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14721..15737)
                     /locus_tag="ACHGMT_000176"
     CDS             complement(14721..15737)
                     /locus_tag="ACHGMT_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN6833955.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15957..17045
                     /gene="chvE"
                     /locus_tag="ACHGMT_000177"
     CDS             15957..17045
                     /gene="chvE"
                     /locus_tag="ACHGMT_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFN6833956.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17099..18625
                     /gene="mmsA"
                     /locus_tag="ACHGMT_000178"
     CDS             17099..18625
                     /gene="mmsA"
                     /locus_tag="ACHGMT_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFN6833957.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18627..19832
                     /gene="mmsB"
                     /locus_tag="ACHGMT_000179"
     CDS             18627..19832
                     /gene="mmsB"
                     /locus_tag="ACHGMT_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFN6833958.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20089..21066
                     /gene="guaC"
                     /locus_tag="ACHGMT_000180"
     CDS             20089..21066
                     /gene="guaC"
                     /locus_tag="ACHGMT_000180"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFN6833959.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21330..22754
                     /locus_tag="ACHGMT_000181"
     CDS             21330..22754
                     /locus_tag="ACHGMT_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFN6833960.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(22796..23053)
                     /locus_tag="ACHGMT_000182"
     CDS             complement(22796..23053)
                     /locus_tag="ACHGMT_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6833961.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            23215..24324
                     /gene="pdhA"
                     /locus_tag="ACHGMT_000183"
     CDS             23215..24324
                     /gene="pdhA"
                     /locus_tag="ACHGMT_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFN6833962.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24327..25304
                     /locus_tag="ACHGMT_000184"
     CDS             24327..25304
                     /locus_tag="ACHGMT_000184"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFN6833963.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            25329..26972
                     /locus_tag="ACHGMT_000185"
     CDS             25329..26972
                     /locus_tag="ACHGMT_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFN6833964.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26980..28386
                     /gene="lpdA"
                     /locus_tag="ACHGMT_000186"
     CDS             26980..28386
                     /gene="lpdA"
                     /locus_tag="ACHGMT_000186"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFN6833965.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            28548..28823
                     /locus_tag="ACHGMT_000187"
     CDS             28548..28823
                     /locus_tag="ACHGMT_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFN6833966.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28829..29596
                     /locus_tag="ACHGMT_000188"
     CDS             28829..29596
                     /locus_tag="ACHGMT_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFN6833967.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29764..31593
                     /gene="typA"
                     /locus_tag="ACHGMT_000189"
     CDS             29764..31593
                     /gene="typA"
                     /locus_tag="ACHGMT_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFN6833968.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31854..33626
                     /gene="recQ"
                     /locus_tag="ACHGMT_000190"
     CDS             31854..33626
                     /gene="recQ"
                     /locus_tag="ACHGMT_000190"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFN6833969.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            33740..34042
                     /locus_tag="ACHGMT_000191"
     CDS             33740..34042
                     /locus_tag="ACHGMT_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFN6833970.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            34045..35208
                     /locus_tag="ACHGMT_000192"
     CDS             34045..35208
                     /locus_tag="ACHGMT_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFN6833971.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            35234..38662
                     /locus_tag="ACHGMT_000193"
     CDS             35234..38662
                     /locus_tag="ACHGMT_000193"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFN6833972.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            38683..39807
                     /locus_tag="ACHGMT_000194"
     CDS             38683..39807
                     /locus_tag="ACHGMT_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFN6833973.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39788..40234
                     /locus_tag="ACHGMT_000195"
     CDS             39788..40234
                     /locus_tag="ACHGMT_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFN6833974.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            40218..40556
                     /locus_tag="ACHGMT_000196"
     CDS             40218..40556
                     /locus_tag="ACHGMT_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFN6833975.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            40675..41235
                     /gene="rsmD"
                     /locus_tag="ACHGMT_000197"
     CDS             40675..41235
                     /gene="rsmD"
                     /locus_tag="ACHGMT_000197"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFN6833976.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            41232..41723
                     /gene="coaD"
                     /locus_tag="ACHGMT_000198"
     CDS             41232..41723
                     /gene="coaD"
                     /locus_tag="ACHGMT_000198"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFN6833977.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            41713..42756
                     /locus_tag="ACHGMT_000199"
     CDS             41713..42756
                     /locus_tag="ACHGMT_000199"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFN6833978.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            42915..43643
                     /locus_tag="ACHGMT_000200"
     CDS             42915..43643
                     /locus_tag="ACHGMT_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFN6833979.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            43759..44004
                     /locus_tag="ACHGMT_000201"
     CDS             43759..44004
                     /locus_tag="ACHGMT_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFN6833980.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            44001..44219
                     /locus_tag="ACHGMT_000202"
     CDS             44001..44219
                     /locus_tag="ACHGMT_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFN6833981.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            44355..44894
                     /locus_tag="ACHGMT_000203"
     CDS             44355..44894
                     /locus_tag="ACHGMT_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFN6833982.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            44887..47160
                     /locus_tag="ACHGMT_000204"
     CDS             44887..47160
                     /locus_tag="ACHGMT_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFN6833983.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            47198..48244
                     /gene="holA"
                     /locus_tag="ACHGMT_000205"
     CDS             47198..48244
                     /gene="holA"
                     /locus_tag="ACHGMT_000205"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFN6833984.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(48333..48587)
                     /gene="rpsT"
                     /locus_tag="ACHGMT_000206"
     CDS             complement(48333..48587)
                     /gene="rpsT"
                     /locus_tag="ACHGMT_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFN6833985.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(48987..49256)
                     /locus_tag="ACHGMT_000207"
     CDS             complement(48987..49256)
                     /locus_tag="ACHGMT_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6833986.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(49518..49646)
                     /locus_tag="ACHGMT_000208"
     CDS             complement(49518..49646)
                     /locus_tag="ACHGMT_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6833987.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(49726..50181)
                     /locus_tag="ACHGMT_000209"
     CDS             complement(49726..50181)
                     /locus_tag="ACHGMT_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFN6833988.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(50319..52247)
                     /locus_tag="ACHGMT_000210"
     CDS             complement(50319..52247)
                     /locus_tag="ACHGMT_000210"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFN6833989.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(52444..53277)
                     /locus_tag="ACHGMT_000211"
     CDS             complement(52444..53277)
                     /locus_tag="ACHGMT_000211"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFN6833990.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(53469..54173)
                     /locus_tag="ACHGMT_000212"
     CDS             complement(53469..54173)
                     /locus_tag="ACHGMT_000212"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFN6833991.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(54196..54822)
                     /locus_tag="ACHGMT_000213"
     CDS             complement(54196..54822)
                     /locus_tag="ACHGMT_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFN6833992.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            55148..57523
                     /locus_tag="ACHGMT_000214"
     CDS             55148..57523
                     /locus_tag="ACHGMT_000214"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFN6833993.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(57547..58038)
                     /locus_tag="ACHGMT_000215"
     CDS             complement(57547..58038)
                     /locus_tag="ACHGMT_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFN6833994.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            58225..>59135
                     /locus_tag="ACHGMT_000216"
     CDS             58225..>59135
                     /locus_tag="ACHGMT_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN6833995.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRS"
BASE COUNT        19374 a        10244 c        12731 g        16786 t
ORIGIN      
        1 gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc aataatagga
       61 acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa aaatagagga
      121 attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga tgttgcagca
      181 agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg atacgataat
      241 gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct ttgtgttctg
      301 tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct cggcggattt
      361 ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg atcgacagca
      421 aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa agctgacctt
      481 gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa atttttagtt
      541 aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta taagattttc
      601 gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca tatttttgtt
      661 gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa aacgatgtat
      721 ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac ttttgtatga
      781 aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga tagaatattt
      841 agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa aaggagaatt
      901 gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa taaaaaatca
      961 ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa ttatccctct
     1021 ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag gtgttcccga
     1081 aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc agttggatga
     1141 tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg aagaaatcag
     1201 tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat ttgctaaggt
     1261 cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac aaattttgct
     1321 tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga atgaacatat
     1381 ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc aagaagtata
     1441 taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt cgtaaaagta
     1501 tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa aaaagcagtc
     1561 catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt tgagatacaa
     1621 cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga caagctgctt
     1681 gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga agtataacat
     1741 gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt tatgggcagt
     1801 tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct ctttagaagg
     1861 atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga gatgcggtgc
     1921 gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag aagggaaacc
     1981 agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt tgccaggtgt
     2041 actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga aagtcaaccc
     2101 caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc cagttgctca
     2161 tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa aaaataatca
     2221 gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg cagggattac
     2281 gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag tagaagactt
     2341 gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact tcggaaaggt
     2401 gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga aaaaaagaat
     2461 ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag atatcttagg
     2521 aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg aacattgttc
     2581 ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc atgtgttgca
     2641 aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag ttgtcttcaa
     2701 agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg cgacaggagt
     2761 tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt tgctagattc
     2821 attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag aagtagttgc
     2881 aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg aaattgcatt
     2941 tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt tgattgaaca
     3001 caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt atgtaggtgc
     3061 aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat ttaatcaaga
     3121 agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa agctgctgct
     3181 tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta tacaggatat
     3241 gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg gctcaggact
     3301 tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt atgaaatgat
     3361 gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg aagaaaaaat
     3421 ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag taacagacga
     3481 cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag tcgatgcatt
     3541 agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa tcgtagaatt
     3601 tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat tgcttgatct
     3661 gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg attcacaagt
     3721 acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg ttagagggac
     3781 gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt tggatccaaa
     3841 aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta gcggtggtaa
     3901 gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag aagttttttg
     3961 ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag gaactcctgt
     4021 tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct atccaacacc
     4081 aatgattggt atggtcggat tgattgagga tcataaacat atcactactc aggaattcaa
     4141 aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg atggcagtga
     4201 attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg atctatccat
     4261 tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga ttgaaagtgc
     4321 tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt tcaaacatca
     4381 gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag aaactcaatc
     4441 aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa tgatgggaga
     4501 tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt cagcaactga
     4561 cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg caatcgtatg
     4621 tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg gggtcatcca
     4681 gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg acaagaaggt
     4741 gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt ggggctgctt
     4801 tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc tgcaatcgga
     4861 catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc ttttctattc
     4921 aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa tgctcgttcc
     4981 cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga tacggagata
     5041 ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt aaaaagtgct
     5101 ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc aatgattgcc
     5161 gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa cggagcatac
     5221 gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat aagagatgtc
     5281 cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg ctttacgaag
     5341 gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc agattcaaac
     5401 atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt agcgaaagaa
     5461 tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc agccgcaagc
     5521 ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa tcaatacatt
     5581 gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg aatgaaatta
     5641 tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga ttcaattgtt
     5701 cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc aaaagaagtc
     5761 catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat tgatattcaa
     5821 acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga atgtatcggt
     5881 gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg tctggaaaaa
     5941 gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc gacgccgtta
     6001 tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt cttttaaaag
     6061 cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag ctggcgtaga
     6121 tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa aaacggaacg
     6181 tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat cggtctttca
     6241 attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat tagcagtggc
     6301 aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt gtgtcaatga
     6361 tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa cggggaaaaa
     6421 tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg ttcaaggaaa
     6481 tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag gtaatgatta
     6541 tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga caggagaaac
     6601 gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt caaatgggta
     6661 ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat caaaactgcc
     6721 agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct atgtttcttc
     6781 cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca ctggtggagg
     6841 gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa tcaaacttgg
     6901 ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa ttcctgagat
     6961 ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc aagaatcgaa
     7021 agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg aaatcgggaa
     7081 agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat gagaatcgct
     7141 gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct atcgaaaaaa
     7201 ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata tgttttgaaa
     7261 cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt tgattcaaaa
     7321 aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga tttgatcgta
     7381 ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga taaaagaatc
     7441 atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat acgtgatgcc
     7501 tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca aggtgtagat
     7561 actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac gttcgattct
     7621 ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat tagtgaaatt
     7681 attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca gataaaactg
     7741 ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt tcaacaggag
     7801 gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa gaagtgacag
     7861 ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc catggtggtc
     7921 ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa aatatccaac
     7981 caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg aaaccagatg
     8041 tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg ctccgtagtg
     8101 cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat gaagaagtac
     8161 tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa ctagcggcaa
     8221 aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta acggattggg
     8281 tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa acgcttcgtt
     8341 acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg gaaaaatatt
     8401 cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat atccgagatg
     8461 cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc gccttgaaac
     8521 acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg gaagcagcgt
     8581 atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga gaagtagatc
     8641 tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc ccttcttata
     8701 cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg acagtagact
     8761 ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt ggacttcttg
     8821 tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg acaaaacgta
     8881 agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc aaacatgtga
     8941 aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga gccgggcaga
     9001 tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa atggaaggtg
     9061 cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat gcagccaaac
     9121 atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat tctattgata
     9181 tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt agacattaga
     9241 gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa catgcaataa
     9301 gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca ggaaatccgg
     9361 ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat tcagcgttaa
     9421 tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa gttcctttgt
     9481 taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt ccgacaaaag
     9541 cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat acttatcaaa
     9601 tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg tatatcaaag
     9661 aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa ggtgtagtcg
     9721 tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac cagcatcgat
     9781 ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa gaattctccc
     9841 ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa gatcataagc
     9901 gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat tcaccagtac
     9961 cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa ccagcggctt
    10021 tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt ttgattgcta
    10081 cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct gaaacgcaag
    10141 tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta ttagagaatc
    10201 gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg gttgcagctg
    10261 ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg gaagaagagc
    10321 tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga gcaggaggac
    10381 gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa aaaatttaca
    10441 ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt ggagaaaaag
    10501 ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa aaaatacaac
    10561 aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt ggtacacttt
    10621 tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg tacttctgat
    10681 cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga agatccagta
    10741 aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc agatatttat
    10801 ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc aatcaaacga
    10861 gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt tgatttttct
    10921 aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat ggattatgtg
    10981 gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt agctgaagcg
    11041 tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa tcaaaaacca
    11101 atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa tcagctgcaa
    11161 tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat gacagatgaa
    11221 gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga tatgacgatc
    11281 caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg gaacgaaaaa
    11341 ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac gacaccaact
    11401 ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat cgccggtacg
    11461 atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat gtctcgggaa
    11521 gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt gtcataagca
    11581 ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt cagcttcgag
    11641 atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt tcgtttatta
    11701 gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa tcccttgttt
    11761 tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa aagcaatatc
    11821 tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc tttgttctgt
    11881 cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga aatgagaggg
    11941 acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg taaaattttc
    12001 tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg aaggctgtga
    12061 gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa gcgagtcgtc
    12121 ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt gcgggatttt
    12181 cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac gatataatcg
    12241 tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag atacttcgtt
    12301 tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat caactggaag
    12361 tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg aactggcggt
    12421 ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc tttctgcgac
    12481 aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat ggaccaacgt
    12541 ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa ctattaaaaa
    12601 tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc taaataaaaa
    12661 cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt ggtttgctgc
    12721 taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag caagtttcaa
    12781 tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat tttttttcaa
    12841 ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta aagcagttac
    12901 tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca gataatcatc
    12961 tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac taataaatat
    13021 gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc cgtccatgaa
    13081 aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg aagacagtgc
    13141 catctcacca tctccagctt cgccgacata agtgattgga taataagaac caaggacagt
    13201 tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa aaactttata
    13261 catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg acagttgttc
    13321 cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa taaagaagga
    13381 gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg atccctttgg
    13441 ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg ttgtctataa
    13501 ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat cctttgtgac
    13561 ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag atcattttta
    13621 aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc atatggccgt
    13681 atcttatttt taagatgact agataatcat tgatatggct aatttctttt tcaagaggaa
    13741 tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca tgggtcatag
    13801 tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc aaactgttat
    13861 aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt gctaaatggt
    13921 attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt ttattcatgc
    13981 tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag ccaaggttcc
    14041 ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg acctttctct
    14101 ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact ccaagttcca
    14161 ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt gctaagtcga
    14221 tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca gaatctacct
    14281 gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta ttgatcagct
    14341 cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac gtactaattt
    14401 ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt tcttctagga
    14461 tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc cacatacttt
    14521 ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata tttgcgatcg
    14581 tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc aaagggataa
    14641 cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg cttaaatttt
    14701 ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat attctgctgg
    14761 gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt ttgaatattt
    14821 ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt acactcgact
    14881 acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc atcaatagtt
    14941 acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc taaagcaccg
    15001 tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc atttgtttct
    15061 aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac aattcggtga
    15121 acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt tggtttacga
    15181 ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt ctctgaacca
    15241 gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt gctgacaaat
    15301 aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac gaaaagctcg
    15361 ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc tcgctttgct
    15421 tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat catatccact
    15481 ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat gtagccattt
    15541 cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc agtatgcttt
    15601 ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg gtcaggtgct
    15661 tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag ggctaaaaaa
    15721 aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta tcttatcaga
    15781 taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa acttaaaaaa
    15841 atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa aaaatcaatg
    15901 ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga taaaaaatga
    15961 aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc attctattaa
    16021 gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga atcgctatgc
    16081 cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg gaattagaaa
    16141 aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat caagtagcac
    16201 aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct atcgatggtt
    16261 cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg atcgcctatg
    16321 atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat aattttggag
    16381 taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa ggaaaaggac
    16441 cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta atcaattaca
    16501 acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc gtgccatctg
    16561 gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg gcgcaagcaa
    16621 ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct gttttgtcgc
    16681 catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg tatgggagtg
    16741 aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc gttaaatcga
    16801 tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta gcaaaaaata
    16861 cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat gatactgaaa
    16921 cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta tctgtcgata
    16981 aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc gatctgaaaa
    17041 attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga taagaattat
    17101 ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa tccgagcgtt
    17161 agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg gagaaaacgg
    17221 agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg gctcctatga
    17281 cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag atagtgaaga
    17341 aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt caataaaaga
    17401 gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg aactgacaga
    17461 aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga atcttctagt
    17521 atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat tatcaaaatc
    17581 cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag aaagcgcgaa
    17641 tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca ttatttcaca
    17701 taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg atggacagac
    17761 gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg gaatggtggg
    17821 cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag tttattttaa
    17881 agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa acgaccacat
    17941 caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg gagcgggcag
    18001 aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat ccggacgtat
    18061 attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg caaacggact
    18121 cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg atattcggga
    18181 aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag ataaagaaaa
    18241 agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa actccatcca
    18301 tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat ccaagtggct
    18361 gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag atgttggggc
    18421 aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag ctgtctgtct
    18481 tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata cgatgaacga
    18541 aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct tgatgaaatt
    18601 aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg gataaaaaag
    18661 agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac agcatggtga
    18721 tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt ttctggcgtc
    18781 cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta gctgcaggga
    18841 tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg atggcgtttg
    18901 ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg atgacagtta
    18961 tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg gtagcttatg
    19021 cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt ggactgacac
    19081 aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa aaaatgtcta
    19141 cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca ttgattttag
    19201 gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat cgtcggaaaa
    19261 atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta tttacagcag
    19321 tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg attattttga
    19381 tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca gtagcaggtc
    19441 gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg atcaaaacaa
    19501 agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta gctggattag
    19561 ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg gaactggatg
    19621 caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga acaatcatgg
    19681 gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg attttaggcg
    19741 tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc gttgtgctag
    19801 acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca gaaaaaagac
    19861 tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca ttgtttcgtt
    19921 tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc tttttgctct
    19981 tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc ttgattaaat
    20041 taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat gaaagtattt
    20101 gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg ttctgaatgt
    20161 gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc tgccaacatg
    20221 cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta tttctatatc
    20281 atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa aaagcgtgga
    20341 ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat agagaagttg
    20401 gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg acatgctaac
    20461 tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt tgtgattgcc
    20521 ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc agatgcaaca
    20581 aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg ctttgggact
    20641 ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa gccgatcatc
    20701 gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt tggtgcgaca
    20761 atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga aacaaaagtc
    20821 gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca aaaaggcgaa
    20881 aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt gaaagataca
    20941 ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg aagagatctg
    21001 gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa tggagacaca
    21061 atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca ttaagtaata
    21121 agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt attatcgcag
    21181 atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt tttgtcacag
    21241 tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta aaatcaaaag
    21301 aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa aaacaaggac
    21361 cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga aggatcaatc
    21421 ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg aatcgttcca
    21481 ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc aaacgtggca
    21541 gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga atcgattggc
    21601 ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa ataagagaag
    21661 caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg ccagatgagc
    21721 ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg gaagaaaaag
    21781 aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat ttggaaagta
    21841 aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt cagcaagcaa
    21901 tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta gagtcacctt
    21961 ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt gggataccga
    22021 tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa aataaattga
    22081 aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg agtcaaaaac
    22141 gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt gaagacgatg
    22201 tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga acgattgatc
    22261 cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca acgaaaatcg
    22321 gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa gaaatgatgg
    22381 agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat gaagcaattg
    22441 atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg ataagcgctt
    22501 ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac tatgcctgga
    22561 ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta gatatgggtc
    22621 ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga atcaattaca
    22681 gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt ctagaaaagt
    22741 ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct tggatttaaa
    22801 tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact aagaaaaaac
    22861 cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa caaaactgta
    22921 taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa aatagaaaaa
    22981 atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat gctgttaata
    23041 tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt gtatttctca
    23101 taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt tattcactgt
    23161 ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg acaaatggca
    23221 aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc agatttccca
    23281 gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt acctgattta
    23341 tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt attagaccaa
    23401 cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac tgctggacaa
    23461 gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct tcttccaggt
    23521 tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc ttttttatgg
    23581 tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct accaccacaa
    23641 attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt gaagaaacga
    23701 aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca aggtgatttt
    23761 tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta tatccaaaac
    23821 aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac acttgcacaa
    23881 aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc gcttgcagta
    23941 tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc agtcctaatc
    24001 gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc aacccgctac
    24061 cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg cttccgtaaa
    24121 tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat cgaagcaact
    24181 aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca aaaggtttca
    24241 gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca aattgcaatt
    24301 tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa gcaatcacag
    24361 atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt gaagatgttg
    24421 ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc ggagaagacc
    24481 gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc ggtcttgcgt
    24541 tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc gaagcaatgg
    24601 acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact agaaatctac
    24661 ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg cattcggata
    24721 atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct tccaaccctt
    24781 atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt gttttcctag
    24841 aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct tacgaagttc
    24901 ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc acatacggag
    24961 caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat atatctgttg
    25021 aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt caatccgtgg
    25081 aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc gttgccgcgc
    25141 aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca atcggacgag
    25201 tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg ttgccaaatg
    25261 caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag aagggacgaa
    25321 acaaagtcat ggcttatcaa tttaaattgc cggatatcgg tgaaggaatc gcagaaggtg
    25381 aaatcgtcaa atggttcgtc aaaccaggag atacgatcaa tgaagatgat acattactag
    25441 aagttcaaaa tgacaaatca gtagaagaaa tcccgtcacc tgttacagga acggttaaaa
    25501 atgtgattgt tccagaaggt accgtagcaa atgttgggga tgtattagta gaaatcgatg
    25561 caccaggaca cgaagacaat gaaggggaca gtggtgtagc cgcagaatcc caaactccag
    25621 ctaaaccggc tgctgaaccc actgttgata cagaatcagc tggatcgtca agcgaaggag
    25681 tcttccaatt taagttaccg gatatcggtg aaggaatcgc agaaggtgaa atcgtcaaat
    25741 ggttcgtcaa accaggagat acgatcaatg aagatgatac attactagaa gttcaaaatg
    25801 acaaatcagt agaagaaatt ccgtcacctg tcacagggac agttaaaaac gtgattgttc
    25861 cagaaggcac tgtagcaaat gttggggatg tattagtaga aatcgatgct cctggacaca
    25921 atagtgcacc gtcaactagt gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat
    25981 caggttcagc aagtgtcgtt gaagctgccg atccaaataa acgtgtattg gctatgccat
    26041 ctgttcgtca atttgcccgt gaaaaagatg tagatatctc acaagtaact gcaactggaa
    26101 aaggtggcag agtaacaaaa gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg
    26161 caccagcgaa atcagaagcg ccagaagcgg cagcaccgaa agaagcagct cctgcagcag
    26221 aaagcaaacc agctgcacca gcaaaaccat tcaagtccaa cttaggagac ttggaagaac
    26281 gtgtagcaat gacgccaaca cgtaaagcta tcgctaaagc aatggtcaac agtaagcata
    26341 cagctccaca cgtgacattg catgatgaag tagaagtgtc taaactatgg gataatcgga
    26401 aacgatttaa agaagttgct gctgcaaatg gtacgaaact tactttcttg ccatatgtcg
    26461 tgaaagcttt aacagcgact gttaagaaat acccagtatt gaatgcatct atcgacgatg
    26521 caaaccaaga aatcgtttat aaacattact acaatatcgg tattgcaact gatacggatc
    26581 acggactata tgtaccaaat gtgaaagatg cagaccgcaa aggaatgttt gctattgcag
    26641 acgaaatcaa tgagaaagca aaattagctc atgacggcaa actatctgca gaagatatgc
    26701 gcaatggaac aattacgatc agcaatatcg gatctgtagg tggcggttgg tttacaccag
    26761 tcatcaacta tccagaagta gctattttag gtgtaggaac gattgcacaa caaccaatcg
    26821 taaatgctga aggcgaaatc gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc
    26881 accgtatcgt agatggagca actgcgcaac aagcaatgaa taatatcaaa cgtcttttag
    26941 ctgatccaga gctattaatg atggaaggat gataaacaaa tggtagttgg agattttgct
    27001 gtagaattag atacagttgt aattggtgcg ggacctggtg gctatgtagc tgccatccgc
    27061 gctgctgaaa tgggacaaaa agtagcaatc attgaaagag agtatattgg cggtgtttgt
    27121 ttgaatgtcg gctgtattcc ttcaaaagca ctgatagctg caggacacca ttatcaagag
    27181 tcactagatt ctgaaatgtt tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa
    27241 acacaagaat ggaaagaaaa caaagttgtc catacgttga cttctggtgt tggtttccta
    27301 ttaaaaaaac ataaggttga aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca
    27361 ctacgcgtca ttcatccaga ttcagctcaa acttattcgt tcaataacgc aatcatcgca
    27421 acaggttccc gtccaatcga gattccagga tttaaattcg gaggacgtgt gttagattct
    27481 actggcggat tagcattgaa agaagtacct aagaaatttg tgatcatcgg tggcggtgtg
    27541 attggtgccg aattgggcgg agcgtacgca aacttaggag cagaagtaac gattcttgaa
    27601 ggctcaccac aaattttacc aacttacgaa aaagatctag taaaattagt agaagatgat
    27661 ttcaagaaaa aaggcgtaac agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac
    27721 ggtgacagcg tgacagtgaa atatgcagtg gacgggaaag aagaatccgt tactgctgat
    27781 tatgtgatgg tcactgtagg tcgtcgtccg aatacagatg atatgggctt agaacaagcg
    27841 ggtgtcgaag taggagaacg aggattgatc acggtggata aacaaggacg tacgaacgta
    27901 cctaacatct atgccatcgg tgatatcgtt ccaggggcgg cattagcaca caaagccagt
    27961 tatgaagcaa aaatcgctgc tgaagcaatc tctggcaaaa aagtagctgt agattataaa
    28021 gcaatgccgg cagtagcctt cactgatcct gaattggctt ctgtaggtat gacgatcaaa
    28081 gaagcaaaag atgctggtat cgaagcaaaa gcatataaat tcccattttc agggaacggt
    28141 cgggcattat cgcttggtaa aacagaagga ttcatccgtc tagttacgac gatcgaagat
    28201 aacgtgctta ttggcgcgca aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta
    28261 gctttagcaa tcgaatcagg aatgaatgct gaagatatcg ccttgacgat tcatcctcat
    28321 ccatctcttg gcgaaatcgt catggatgct tcggaattag cattaggttt accaatccat
    28381 atttgatttg aataatacag gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta
    28441 gaattatcaa taatattatt atgtaaactt aaaaatatga caacttcttg gtttattacc
    28501 acttcctatg ctacactgaa gaggaatatg aaataggaga gttgaagatg caagaatatc
    28561 agtatccact tgatttagat tggacaacag acgaaatggt cattgtaatg aagatgtggg
    28621 aagatctgga acgtgcgaat gaaaaaggga taaacaatga agcttttttg aagacttatc
    28681 agcagtttaa aactgttgtc aaatccattg gtgaggaaaa acgattggga cgggaatttg
    28741 aaaaagcttc cggttattct ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca
    28801 aaactttgag aatgaacggg tgagataaat ggaacagatg attgatcata tcaagtcctg
    28861 gcttgaagaa gctggtgaga aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa
    28921 atcaaatcga acggatctag taacaaatgt cgatcgaaac atccagaatt ttctaatcga
    28981 aaaaatccat tcctttgatc cagaggcgaa aatattggca gaagaagaag ggtgtaatca
    29041 attatcggat ctcgatggaa gagtctttat catcgatcct attgatggca ccttgaattt
    29101 tgtgattgaa ggggaaaatt tttgtattat gctcgctgtt tatgaatccg gcgttggaaa
    29161 gcttggtttc atttatgacg tgatgagaaa tgaacttttt tggggtggaa aaggaatagg
    29221 cgtgtataaa aatgatgagc cattgcctag accagcaaat aaatccttat cggaaggctt
    29281 acttggtgtg aatagttatc ttttcggtca taataaatat ggaatccgag aaattgcgga
    29341 aagcagcatg gggacacgta tgtacggatg cgctggtctt gaactagtgg caatcttgaa
    29401 aggaaaccac attgcttata tttcaaacct tagcccttgg gattatgcag cgggaaatgt
    29461 cttattggaa gagtttggta tggtaacaag cggtcttttt ggtactcctt tgacatttga
    29521 aaaaagggaa tactatttag cagggacttc ttgtgcatat gcagaaatcg cagacctttt
    29581 gaaaaaagaa ggctgaaaaa aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa
    29641 agattaattt tctcgaaaaa atgaaaacat tttactatat ttcatgaaaa aagtctgcta
    29701 taattagcag tcgagtaaaa tttgccctgt cataaagaac caataaaaaa ggagttagtt
    29761 acattgaatt atagaaacga tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga
    29821 aaaacaacat tagttgatga actattaaaa caatcagata cattagacgc acatacgcaa
    29881 ttgcaagaac gtgcgatgga ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta
    29941 gccaaaaaca cagctgtaga ctataaaggt attcgcgtaa acatcatgga tactccagga
    30001 cacgcggact tcggtggcga agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg
    30061 gtcgtagatg cgtatgaagg aacaatgcct caaacacgtt ttgtattgaa aaaagcactg
    30121 gaacaacata tcacaccaat cgttgtcgta aacaagattg acaaaccttc tgctcgtcct
    30181 gagcatgtcg tagatgaagt tcttgaacta ttcatcgaat taggagcaga tgatgatcag
    30241 ttagactttc ctgtgattta tgcatctgca ctaaatggta cgtcaagctt gtcagatgat
    30301 ccagcagatc aagaaccaac catggcacct atttttgata caattattga aaaaatccct
    30361 gctcctgtcg ataacagcga cgaaccatta caattccaag tttcattact tgactacaac
    30421 gattatgtag gtcgtatcgg aatcggacgt gtatttagag gaacaatcaa agtaggagac
    30481 caagtagcat taattaaatt agacggaaca gttaagaaat tccgtgtaac gaaattattt
    30541 ggtttctttg gactaaaacg tctagagatt caagaagcaa aagctggaga cttgattgct
    30601 gtctcaggta tggaagatat ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat
    30661 gcgttaccta tccttcacat tgacgaacca acattgcaaa tgactttctt agttaataat
    30721 tcaccatttg ccggtcgaga aggtaaattc gtaaccgctc gtaagattga agaacgtttg
    30781 atggcagaac ttcaaacaga tgtatcctta cgagttgaac caacaaactc tccagatgca
    30841 tggacagttt ctggtcgtgg agaattgcat ttgtctatct tgattgaaaa catgcgccgt
    30901 gagggatacg aattgcaagt atctcgtcca gaagttattg aaaaagaaat cgacggtgta
    30961 aaatgcgaac cgtttgaacg cgtgcaaatc gatacaccag aagaatatat gggtagtgtt
    31021 atcgagtcct tgagcttacg taaaggtgaa atgcaagata tggtccatac aggaaacgga
    31081 caaatccgtt tgacattctt aacacctgcc cgcggtttga ttggttattc aactgaattc
    31141 ctttcaatga cacgtggtta cggaatcatg aaccatactt ttgaccaata tctgccaatg
    31201 cttcctggtc aaattggcgg acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa
    31261 gcaacgactt attctatcat gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt
    31321 acagaagtat atgaaggaat gattatcggg gaaaacagcc gtgataatga tttgactgtc
    31381 aacatcacga aagcaaaaca aatgacaaac gtacggtctg ctactaaaga ccaaacatct
    31441 gtcatcaaaa aaccaaaaca attgacgttg gaagaatctt tggaattctt gaatgatgat
    31501 gaatattgcg aagtaacacc agaaagcatt cgcttgagaa aacaaatttt agagaaaaat
    31561 gctcgtgaaa aagcatctaa aaagaaaaaa taatctgtaa aaggatgaag agagaaacca
    31621 gcttgttata aaaaatagct ggtttctttt tttgtccaac tcttttctgt ggagaagtca
    31681 tctttgtcaa tggacataaa ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa
    31741 aaacttattt ctagagagtt tttgtttcat aataagataa tgtttcacga aaggagatca
    31801 acttgtcctt cgaagaaaat cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg
    31861 acttattaaa acaatacttt ggctatgatg agtttagacc tggtcaaaaa gaaattatcc
    31921 agaaagtcat tgatcaggaa aatgtattag gaatcatgcc taccggaagt gggaaatcaa
    31981 tctgttacca gcttccagct ttactattag atgggcttac tgtggttgtg tcccctttaa
    32041 tctctttgat gaaggatcag gtggacgcgg cgaatcaatt aggtataccc gccactttta
    32101 tcaatagctc gttagatggt tacgaaacag ctagacgctt ccaagaaatc gatagacagc
    32161 agtaccgttt actttatatc gctcctgaac gttttatcat gccggatttt atccaagcaa
    32221 tgaagcgatg gaacgtccgt atgatcgcta ttgacgaggc acattgtatc tctcagtggg
    32281 ggcatgattt ccgcccaagt tatttacaga tggcgaacca gctagatcaa ctgccaaatc
    32341 gtcctgtaat tgttgctttg acagctacag cgactgtgca agtagctgct gacataaaaa
    32401 gacttttaaa aattccagaa aacaaccata ttcagactgg ttttgaacga gaaaatctac
    32461 gatttcaagt aattaaagat caaaaaaaag aacaatatct aatcgaatat ttaaagatca
    32521 acaaaaatca atctggtatc atttatgcag caacgagaaa agaagtagac cgtctctatc
    32581 atttgcttaa aaaattcgat ttttccgtgg gtagatatca tggtggtcta aatgagaatg
    32641 agcgaacaga aatgcaagaa gcctttttat acgatcgctt gcagctgatt gtagcgacaa
    32701 atgcatttgg tatgggtata aataaaagca atgtccgttt tgttatccat tatcaaattc
    32761 ctggctctct agaagcatac tatcaggaag ctggtcgggc gggaagagat ggtctttcca
    32821 gtgaagccat cctgcttttc tctcctcaag atatccaagt ccaaaagttt tttatccagc
    32881 agtcccaaag agaagaaggg caaaagcaaa aagaatatga aaaattaaaa gcgatgacag
    32941 aatacgtgta tattgagtcc tgtcttcagc aatacatcct aaactatttt ggcgagacaa
    33001 gcagtccgtg caaccgttgt ggtaactgtt tagatgacag agaaatagta gaagtaacga
    33061 cagaagcaca gatggttctt tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa
    33121 tgctgatgaa agtcttagca ggatcgaaag aacaaaagct gcaagcttta ggttttggac
    33181 atttatctac ctatggactg atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg
    33241 aatatttgat atcgaatggc tatcttctga ccatcaatgg tgagtatcct gttttgaaag
    33301 taacagaaag agggatacaa gtattgaaag gacaagaatc tgtttatcga aaggaaccca
    33361 agaaagtcca gcagctatcg gatgaagaaa ccgatacact atttgaagtg ctgcgggaac
    33421 tgcgaacgga tctagcatca gaagcaggcg ttcccccgta tgtcgtattt tcagattcca
    33481 cattgaaaga aatgagccga atacgtccat caagcagatt ggagatgctt cagataaaag
    33541 gagttggcca aagcaagctt gataaatatg gcgaagcctt tttatctcgg ataaaaaatg
    33601 cggactctaa cgttctggat aagtagaaag aacgaatacg taaaaatgaa taaaaacaac
    33661 tggttaaatc tgtttttttt gctccttatg aaaatcaaat atgttataat taaggaatta
    33721 gaatgggagg taagcacaga tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa
    33781 tgatgaagca gaacggatca aacgattaat ccgaaatcaa aacaacagtc tctgtatctc
    33841 acaatgcaaa gccttcgaag aagtggtaga tacacagatg tacggctttt cccggcaagt
    33901 cggttttgct gtccgtatag gagtaatcgg aaagaacgag ggccagctga tgttaagtga
    33961 gctagaacga gaattgaatc agttatatag tgaggtttac caagaaattt atgataaaaa
    34021 agaagttggc aaggaggaat agacttgcct aagaaagtga aaaaaaggca tctattagac
    34081 tatagcatct tgattcccta tttaatttta tgtgtattgg gattgatcat ggtatatagt
    34141 tctacgtcgt atctattatt agaaaacgga caaaatccgt ccgcatcagt cattaaccag
    34201 agtatttttt gggtgcttag cttgattgct atcgcactac tttataaaat gaaaacagat
    34261 gttttgaaaa accagcgttt gatcatggca gccatcgcag ttttgacgat tttgctgctg
    34321 attgttgtgt tttttggtaa ggagatcaat ggggcgaaag gttggctgca aattgcagga
    34381 ttctcgatcc aacctgccga atacctaaaa atcatttcta tctggtacct gtctctgact
    34441 ttatctaaaa gacaaaacag tgtacaaaaa gattttcttg ggactgtgaa acgtccactt
    34501 gcaatggtta tcggacttac tgcattagtc gctattcttc ccgattttgg taatgcggca
    34561 gttatctttt tgattatttt ggttctgctt ttagccagcg gagtcaatta tgtgtatact
    34621 ttgattgtag gagtaggagg cttttgtcta agtactttta cgatctggct gatcaatatc
    34681 acaaacggaa agatcttacc cggacgttta cagtatatct ataatcgatt tgctatttac
    34741 caaaatcctt tctcagacga attaaataaa gggcatcaac tagtcaacgg ctactatgcc
    34801 atgttcaacg gaggactatt tggcagggga ttaggtaaca gtatccagaa aaaaggattt
    34861 ttacaagaag cacaaaccga ttttatttat gcaatcgtag tggaagaact gggagtgatt
    34921 atggggattt tgatattagc gttgctattc tttatgattg tgcgcattgt ccttgtaggt
    34981 atccgttcaa aagatccgtt caattcgctt ttgtgcattg ggatcggcgc aatgtttttg
    35041 atccaagtat ttgtcaatct tggaggaatt actggcgtta ttcctttgac agggatcacg
    35101 ttcccatttt taagtcaagg aggctccagt ttgctgatgc tttcgatttg tgtcggcttt
    35161 gtcctcaaca tcagtgcgga tgaaaaacgc aagagcttag gtttataata aaaaaagatg
    35221 taggagtggt tcgatgaaaa aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt
    35281 atttcgtgct tgtacagaat taggaattaa aactgttgga atctatgcag aagaagatga
    35341 atattccgta caccgtttca aagcggatga agcatattta gtaggacaag gaaaaaaacc
    35401 gattgatgcg tatctagata ttgaagggat catttctatc gcgaaagaat gcggagctga
    35461 cgctattcat ccgggttacg gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga
    35521 agaagaaggc atcatttttg ttggacccaa actgcatcat ttagatattt ttggagataa
    35581 aatcaaagca aaagctgcag ctatcgaagc ggggattgct tcgattccag gcacagatgg
    35641 accaatcgct tctatagacg atgccttgga gtttgcgaaa caatacggtt atccgattat
    35701 gatcaaggca gctctaggcg gcggcggacg cggcatgcgt gtagctcacg atgaaaagag
    35761 tgcaagagag ggctacgaac gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga
    35821 agtgtatgtt gaaaaataca tcgctaatcc taagcatatc gaagtacaga ttctcgggga
    35881 tacgcatgga aacgtcatcc atctttttga aagagattgt tctgttcagc gccgtcacca
    35941 aaaagtagta gaagtagcgc cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg
    36001 ccaggcagct gtccaattga tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt
    36061 tcttgttgaa ggagatgatt tttactttat cgaagtcaac cctcgcgtcc aagtggaaca
    36121 tacgatcaca gaaatgatta cagatattga tatcgtaaca acgcaactgt tgattgctca
    36181 aggtttggat ctgcataaag aaatcggatt gcctcagcaa gaaggaatta aattaaatgg
    36241 ttcagccatt caatgccgaa tcactacaga agacccgttg aataactttt taccagacac
    36301 agggaaaatc gatacgtatc gttcacctgg tggttttggc gtacgtctgg atgtcggaaa
    36361 tgcttacgcg ggctatgtag tgacaccgta ttttgattca ttgctagtaa aagtatgtac
    36421 gcatggtgct acctttgaaa cagccattca gaaaatggaa cgttgcttga aagaatttag
    36481 aatccgtgga gtgaaaacaa acattccatt tatgctcaat gtcatcacac atcctgaatt
    36541 ccaatcagga aatgcaaaaa caaccttcat tgatagtaca gcaacattat ttgaatttcc
    36601 tcgactacga gaccgcggaa acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg
    36661 ttttccaggt atcgaaagtg gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga
    36721 tctaatcaca agaactgatt acgtaacggc taagaatgta ttagatgcga agggtgccga
    36781 tgctttggtc gaatggatca aaggacagga aaatttgttg ttgacggata cgacatttag
    36841 agacgcccat caaagtctgc tagcaactcg tgttcggaca aaggatttca agcagattgc
    36901 acgtttgact ggagaaggtc ttcctgaact cttttcaagt gagatgtggg ggggagcaac
    36961 atttgatgtg gcttaccgct ttttgaatga agatccttgg caacggttga gaaaaattcg
    37021 ttctttaatg cctaatacgt tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta
    37081 ttctaattat cctgacaatg tattggtgga atttgtcaaa gaagcagcag cgcaaggaat
    37141 cgatgtattt cgaatctttg atagtttgaa ctggactcca caaatggaaa aaagtatcca
    37201 agctgtacgg gatactggta aaatcgcaga ggcagctatt tgctatactg gcgatatcaa
    37261 cgatccaagt cgggcaaaat acaatgttca gtattataaa gacatggcaa aggaactaga
    37321 acaactgggt gcgcatatca ttgcaatcaa agatatggct gggctgctta aaccgcaggc
    37381 agcttatcgt ttgattagtg aattgaaaga aacgacagat ctgccaatcc atctgcatac
    37441 tcatgatacc agcgggaatg gaatcatcac ttattctgct gcttcaaaag caggagtaga
    37501 catcgttgac gtggcaatga gtgcaatgag tgggaatact agtcagccta gcatgagcag
    37561 cttgtattat gcactggtca atggtccacg gctgccagaa atcacgattg aaaatgcaca
    37621 gaaattaaac cattattggg aagatgtgcg catgtattac aaaccgtttg aaaacggctt
    37681 gaatgctcct gaaacagaag tatatatgca tgaaatgcca ggaggacagt attcgaatct
    37741 acagcaacag gcaaaagctg tcggattagg tcatcgctgg gatgaaatca aacaaatgta
    37801 tcatactgtc aacttgatgt ttggcgatat cgttaaagta actccatcat ctaaagtagt
    37861 tggagatatg gcactattta tggtccaaaa tgatcttaca gaagaagata tttatgaaaa
    37921 aggcgaaaca ctaagtttcc ctgaatctgt tgtgactttc ttccaaggag aactagggca
    37981 acccgttggc ggatttccta aaaaattgca aaagattatt cttaaaggca gacctgcatt
    38041 gaatgaacgt ccaggtcttt tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt
    38101 ggcagaaaaa attggctatg aaccaaaaca agacgaagta ttaagctatt tgatgtatcc
    38161 gcaagtcttt ttggactatc aaaaagcata taatcaattt ggcgatgtta cattactgga
    38221 cacaccaaca tttttccaag gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg
    38281 cggaaaaacg cttatcattc gtttagatga aatcggagaa cccgatatcg aagggaatcg
    38341 cgtactgttc ttcaatttga atggacaaag acgtgaaatc actatcaaag ataattcgat
    38401 catcagtacg gtccaaaccc gattgaaagc tgaaccgaca aaccgagaac aaattggagc
    38461 aacgatgtct ggttctgttt tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg
    38521 tgatacgttg atgattactg aagcgatgaa aatggaaacg gccatcgaag cacgttttga
    38581 cggagaagtc gcacatgtat atgtgtcttc aggagacact atttcgtctg gtgacctttt
    38641 gattgaagtg actgagaaat aataagcgaa aggtggggaa acatgaaaag actgggtttg
    38701 ttttggctga ttctgctatt agttattgtg gcattctaca tggaaccagt cttgtttccg
    38761 ccaaaacttg aagtgaagga aaactctgat caacaaatat caaaccaaat gacaacgtgg
    38821 aaataccagg agcttaaagc aactggtttt tctacttata tcggaaaacc gatcgatcag
    38881 ctggaaaagg attttggcca agcctatgat cgattaacta gcggatttgg ttttgaaaca
    38941 agatattatg aaaataaagc tagtcatctc acatttgaag cgaacatcca agacagcaga
    39001 gtcacaactg ttaaagtact gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg
    39061 atgacgatgc aagatcttac gaaaatcact actatctata ctaatttcac ttttgagtac
    39121 catgatacag aagttggaat cgaactaatg gaagaggata tgaattatcg tccgctaatt
    39181 gcttttgata acgacacttt tgctatttta ttttttgatc aaacaacaaa cggcctattt
    39241 tcagttgtgt atttagataa agatagtctt ttgaaactat tgccatatca agtgtttggt
    39301 gaaggccttc cacattatca agcagataaa tcggctgatt gggaatcaat caatcgcgtt
    39361 aaagaacgaa aaagtacgac tttattaaat atgcttagaa gagaagacga acttcccata
    39421 tacaatcaaa cacttgcctt tcaaaaccac agtcagctgc tactacatga ttttttgaca
    39481 aggcctgagg aaatattgtc agaggaacgt atggctgatt ggcaaaaatc gctaggcagt
    39541 gctcaaacga cagtgaaatt ctcactgacg aatgatgaat tggagaagtt aatcagtaaa
    39601 caaaaattag aaaatactag tgggctattt attcatccgg cgattgacgc tactttttcg
    39661 tttttatatt ggtacagcga tccttacaac catgaccgtt tcatggaccc gggaacggat
    39721 tctgtaggca ttgctttttc taaagaaaat atgttagtct tattgcaaga agaaaacgga
    39781 gaaagtagtg atagcagtga tcgttaacga agagctgttt caactggagg atcaatgcct
    39841 ccaagtcgct gaaatgataa aaaagagtaa aacgatggct tcttatcttg aacataaaca
    39901 gtcaatggaa gaatctgcaa aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga
    39961 agcatttgaa aatgtagcgg catatggtga gtatgcacct gattatcgtg aaaaaaaacg
    40021 agctgttcga aaagcaaaaa gacagcttga tatggatgag catgttgcag ctttccgata
    40081 tagtgaaaca cagctgcaaa gtctgttgga tcaaattgtc caagtacttg ccgaagaagt
    40141 ttctaatgaa atcaaagtag atgcaggtag cccgtttttt gaaacgaaaa aacactttgg
    40201 atgcggaggt aattgtcatg caattagaca atagtgaatt tgaaataaca aaacgacgcg
    40261 ggctgatcgt atgggtctac agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt
    40321 tgcattatgt ctcaagaaga atgaaatatg tcgtgcttta cttaaatgaa gagacattgg
    40381 aagaaacaga agaacgaatc cgtaaattac attttgttcg aaacgtagaa cgttcttacc
    40441 gaccggatat cgaaatgaat tttgccgaaa aaataggaaa caaaacagta ttaaaagacg
    40501 aaggtttcga aattgaagag ctaaatacag aaatccgatt agctgaacat ctttaaaaat
    40561 ctcttatgag cagtttttaa ataaaaaata acttggataa cgatcgattt ttgatcattg
    40621 tccaagtatt tttttggtat actagtacca gtaccatttc taaggagtga gtagatgcgg
    40681 ataatatcag gaaattatgg cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga
    40741 ccaactacag ataaagtaaa agaatccata ttcaatatga ttggaccata ttttgatgga
    40801 gagacagtat tggatctgtt tgcaggaagc ggtggtttag ctatcgaagc agtatctaga
    40861 ggctgcagtc atgcggtatg cgtagacaaa aattaccaag cgctaaaaat catcaaagaa
    40921 aacatcgaaa ttacgaaaga accagagaaa tttaccacaa taaaacttga tgcggacaaa
    40981 gcaatcgaac ggctagcagc ggagaaacaa caattcgact acttgtttct tgatccgcca
    41041 tatgcaaaac aaaaaataat cgatcaaatc cataaaatgg aagaactggg acttttttct
    41101 aaaaccgcag ttatcgtatg cgaaacggat aaaacagtcg aattgccaga aactgtggct
    41161 ggattccatc aaataagaaa acagatttat ggcataacgg ccgtaactat ctatagaaaa
    41221 gagggaaatc aatgaaacgt gcattattcc caggaagttt tgatcctttt acaaaaggtc
    41281 acttagatac ggtggaacgt gcggctaaat tattcgatga ggtagtaatt ggtgttttta
    41341 tcaatacttc aaagaaaagt ttgtttcccc cagaagaaag aatgacattg atcacaaaag
    41401 ctgtttctca tcttcctaat gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa
    41461 cagcaaaaga gattggtgca gatgcgttga tcagaggcat acgaagtatc aaagactttg
    41521 aatatgaacg agagattgct cagatgaatc atcatctata tgatgaattg gaaactgtct
    41581 tcttattagc aaaaccagaa tatagtcacg tgagttccag tattttaaaa gaagtactgc
    41641 actttggcgg agatgtgagc agctacttac cgccagtgat caacgaagct ttagctggaa
    41701 agcgtgaatc aaatgaaagc taaacaatgg ttgaaaacag tactgattat catatttgga
    41761 atgttgatca ttggatttgt agtagttccc ttgccgtatt atgtagaatc accgggagcc
    41821 acgatcgatc tgaaagaact aattacggtc aatgataagg aagataaagg ttctgggtct
    41881 ttctctctta cctctgttgg tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa
    41941 atgacccctt ttgaagaatt gatcagtgaa gaagaattga ctggtggggc gacaaatgaa
    42001 gaatataatc aaatgcaaca gtattatatg gattcttccc aaaatgcagc aatcgaacaa
    42061 gcgttaaaac ttgcagacaa gccttataaa atggaattca aaggcgtata tgtcatgagt
    42121 gtagagaaaa catcgaattt ctttggcaag ctttcagttg gagatacagt aacaaaagta
    42181 gacgggaaaa gctttcaaag tacagaagca tttatggatt atgtaaaaag ccaaaaagtt
    42241 ggacaaacaa tcgaaatcag ttatttgcat aatggtgaag aaaaaacagc ttctggtgat
    42301 ctgatcgagt taccaacaga taaaaaggca ggaataggta tcacgcttac tgaccatacc
    42361 gagatcgaat cagatacaga tgttcgtatt aattctggct ctatcggtgg gccatcagcg
    42421 ggattgatgt tcactttaga aatctatgaa caagtcactg gtaaaaactt gcgacatggt
    42481 aagcaaattg caggaactgg aacgatcaac agtgaaggag aagttggccg aatcggagga
    42541 atcgataaaa aagtggcaag tgcggataaa gctggcgtag agattttctt tgcaccagat
    42601 gatacgattt cagacgatgt taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa
    42661 gcaaaagctg ccgctaaaaa attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta
    42721 caagaagcgc ttgactatct agagaaaatg aaatgaaact aaataattaa agaggggttg
    42781 tgacacgagt tctgtcacaa cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa
    42841 acaaaagaaa aaattgacgt aattagatgt tgaagggacg attttgccag agctcactgt
    42901 ttcttcaatt ttaaatgaaa ggagtaatga atgattttta ctggtcattc tttgaaaaaa
    42961 gaacaatgat agaaaaaatc agcaagaaaa tactagcttc acccaaattg atcatttgga
    43021 tgtctatgat agttcttatc ttttgtatcg caagtggttt tttattatca aatttgctat
    43081 tttatagaga aacagaagag actgctgcaa aagaaatact ttcttcgttt agttccagtg
    43141 gagaagatcc atcagctgaa ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt
    43201 acaccgatat caaaggaagt gtgaaggagc ctggtatcta ttccttttct tcagaagaac
    43261 gggtctatga tgtactaaag cgtgcaggtg gattattaga agaagcggat agcgatcgaa
    43321 tcaacttttc tgcgaagata gaagatcagc aagtcctata tatccctgct gtaggtgaag
    43381 agctgccaga acatcttgat cagttggcat cctcggaagg aaaacagcct acagcagatt
    43441 ccgagcctcc caaaataaat atcaatacag ccactccatc agaacttcag cagattcctg
    43501 ggatcggatc agtcaaagcg caagaaatca ttcgatttag agaagaaaac ggatccttcc
    43561 agaaagtgga agacctgcag gagatttctg gtatcggtga aaagacagtt gaaaaactaa
    43621 aaaacttcgt tacaattaaa tagaatgatt caaaatcgac tggcacgagc aataaatgga
    43681 cgctaatgga tactaagaat aatccaattc taagagcttg gcaggacgag gaggtagtat
    43741 ttttggatag gaaatagctt gattgttcgc aaagaaacat taaaaaaacc aatgctgaat
    43801 gtctatctcc aaaataaaat atctgggatt catattatga atacggctgt cagcggcaat
    43861 aacagtcaag ctctacgaga acgctttgcc aaagatgtac ttagctatac agcagataaa
    43921 gtatttattc tcattgggac gaatgatcta gcagagcata aacaactttc taaagaaacg
    43981 taccaaaaaa tctgcagtgg atgatagttc agctgaaaaa agactatagt tctgatcatg
    44041 ctttctttat cacgccacca gcagtcgatg agacaaaaca aaaatatcgg aataacaagt
    44101 tgatcaaaga atatatctct gttctcacag aagtagcaaa acgccatgat tgtgaagtat
    44161 cgatttttat acggaatttc ttgaacaaat ggattcgaag aatttttata atgggctaat
    44221 ggatgacgga cttcatttcg gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg
    44281 ttatatttaa agaatatttt gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg
    44341 gaggaatata aaacatgtta gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag
    44401 ttactgataa gtcacataat gaacgcattc cttgggacca gtactttatg gctcaggcag
    44461 tactgttatc tttacgtagt acgtgtacac gtttagaagt aggagcgaca ctcgttcgag
    44521 aaaaaaggat cattgctggc ggctataatg gagcagtcag tggagatgtc cactgcatag
    44581 atgaagggtg ttacgtcgtg gatggacact gcttgcgaac gatccatgca gagatgaatg
    44641 cactcctaca gtgtgcaaaa cttggtatcc cgacggaagg ttcagaaatc tatgtcacac
    44701 attttccatg tttggcctgt acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt
    44761 atctccatga ctatcgaaat gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat
    44821 ctgtacacca agttgcttta gactcacagc atttttcaaa acttcaaaat gaattaacag
    44881 gaaaacatgt ctagcgtttt agaaaggcat gttcgaaatc ggttgatttt tccggcaatt
    44941 acgtcgatga ttatctgttt gttgtattac actgacaatt ggggttatcg tttgcttctt
    45001 gccggaattt ttttatggtt ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt
    45061 ttgtgtagca taacggtatc catttcttat aggctacaag agtattcatt tttagaggaa
    45121 aaaacaacat ctgcgcaaat tactgtattt acagatacga ttcgtgcaaa tggtaatttt
    45181 ttgacaatgg aaggaaggtt atcagagaat aaacaaaagc tccagctttc ttatcaagta
    45241 aacacagaag cagaattgaa aacatggctt aacttccgag gaaagggaat agaattgact
    45301 gctgaaggcg agttcatctc tccagcaaaa aagcggaatc tttataattt tgaccaagaa
    45361 gcttacttca ggaaccatag aatttctgga cggttccaaa ttcaaaagat tgaacagatc
    45421 accatgcaaa atcactggcg aaactggctt cagagaaaaa gaggatcttt tatctccttt
    45481 gtccaatctg aatttccgca aaaaacaagt gtatatatta attctttatt atggggctat
    45541 aaagatgcag gattcagaga accagaagaa atttttaggg agagtggttt gctgcatctg
    45601 ttcagtctat cgggtctaca catccaattt tatttagggt ggctgtatta tttgtttcga
    45661 cgtatcggat ggccactaca cgtcagtatt attccgttga gtctactaac tatctgttat
    45721 gtttctattg caggaagttc tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga
    45781 ttgctagtca aattgctgaa taagcattat tccaccatgg acttatttgc agttgtcttt
    45841 tggcttcttt tactatttga tcctcgattt ctttttcatg cgggtggaca gcttagtttt
    45901 ttcatgagtt ttttgtttat cctgatttct tttgatggag attggttgaa aaaaacggcc
    45961 tctcacgtcc tgtttactat gatcacgatg ccgttgattg tatggcattt ttatgagtgg
    46021 cctattttag gtagtctttt tacattactg attgcaccgg tattcaagtt tctttttctt
    46081 cctggtgtgt tatttctcac actcttcaat cgctggctgc cggaaacttt acttttgctg
    46141 atagaagagg gattaatctt atttgagcga ataatcgatt tttcttcagg gtcgtctctc
    46201 attataggac gtttgcccct tgtgctatct gggatgtata ttttcggatt actgatttgt
    46261 atggataggt tgaaagaaca acctgtgaaa cacttgtttt atgttggtct tttttctttt
    46321 atttttttga ctttgccatt tttacgcttt tcttcaacga tcgcttttat cgatgtcggt
    46381 caaggagaca gtatcttttt gcaaagtcct tttcgaaaag aaacgatttt gatcgatact
    46441 ggaggtcagt tacactttga aagagaaaag tgggcgcaag gtatgatacg tcctcctgct
    46501 gaaagaaata tcgttccata tctaaaaggt caggggataa gtgtaatcga taaactcatc
    46561 ttgacacacg acgatacgga tcatgtggga gaattaccca cgttatctca gcattttact
    46621 atcaaaaaaa tttatatcgg ttggggcgct ggacgaaatg agccattaca aaggcattta
    46681 tctgaagctc aaaaaaatgg tactgagatc attgaaatca aacatgggga caggatcagc
    46741 ggttattatg atctttatgt attgactcct tttgaaaagg gagagggaag aaatgaagat
    46801 tcgattggtc tatggatgga atataacaat aggcgctttt tatttttagg agatttgaat
    46861 caagcaatgg aaaaacaatt gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa
    46921 ttaggtcatc acggtagccg tacgtcaagt aacacagatt ttctttccca tattgaagca
    46981 aaacatggga ttatatcctg cggcgtaaat aatcgttatg gtcacccgca cagcgaagta
    47041 ttggagacac tagaagaaaa tcagataaaa acatggcgaa cggatcaaca aggaatgatc
    47101 acctatcgct ggcatccagt ttttcatcct catgggctag tggaaacaat gatagactag
    47161 taattctcat ctgctcatgg taggataatt tttgaaagtg gtgagtaatt tgagtataca
    47221 agcagagtta caaaaaatac gtaaagaaaa acttgcatct tgttacttga ttttaggaac
    47281 agaaaagttt ctccaagatc aagttcggac agaaattgtc aaaaaaatcc aattagaggg
    47341 cgaagatgac ttaaattttt taagttttga catggaaaat gcaagtttag atgaggtagt
    47401 agctgaagca gagacgttgc cgtttttcgg agatcaacgg ttagtattcg tagagaatcc
    47461 ttatttttta acaggtgaaa aagtaaacaa tggaatcgaa caaaatactg atttattgac
    47521 ggcttatttg aaagaaccat tagaatcgac tgtattagta ttctttgcgc catatgaaaa
    47581 gttggatgaa cggaaaaaag taacgaaaca attgaaaaaa acagcaatta tcatcaatgt
    47641 ccaacagttg aatgaaaaag aagtacgtca atatctgatg aataccttag agaattcctc
    47701 aatcaaaatg gaacgaagtg caattgattt attcttgagg ttgaccgact tagatttatc
    47761 caaaatgatg agagagttac agaaattggt cctttatgga caaaatcaac agcaaatcac
    47821 ggtaagagaa gtagaagaat tagttcctaa aacgttagaa cataatattt ttgacatgac
    47881 ccaatatatt ttaaaaggga agacggaaca agctttgcgt ttatacgaag atttagttat
    47941 gcaaggggaa gaaacaatca aaattaatgc gattttgctt tctcaattac gtttattttt
    48001 acagacaaaa tttcttgtaa agattggtta tcagcaggca aatattgctg aaacattgaa
    48061 aatccatcca tatcgtgtaa aacttgccat gcaggaagta aaaaaattcg atgaacattt
    48121 gctggtacag ttgtttgatc aattagttga gatggattat caaatcaaaa cgggacaaat
    48181 cgaaaaagaa ttaagttttc aactattcgt tttgcgcaca gggcagttgt taagaaatcg
    48241 ataagaagaa agacgagggc aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg
    48301 acaaataaaa aaatcggccg aagccgattc ttttatttag cgattttttt gcttaaacga
    48361 gatttgtcgc ggttagcttt gtttttgtgg attagacctt ttgattcagc catatcaata
    48421 gctttaacag cttctttata taacgcatct acgttgtcag cacctgaagc tacagcatct
    48481 tcaaattttt tgattgcagt acgcatagcg cttgtttgag atgaattttt cacattagcg
    48541 ttttcgcttg tacgaacacg tttgattgca gattcaatgt ttggcattag tttcacctcc
    48601 gataacttaa gttcttacgc gtgttaccgc ataattcaac attcattatt atacctaaag
    48661 gcagcgtgct ttgcaatatg aacatgacaa gtttttttcc ttaacagtct tttattagga
    48721 ttttttttca gtaaaaacgg atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg
    48781 gaggcaatag cgtcaaacca aactagattt ttatttatct atttaaacaa agtttgaatg
    48841 ggaatgtttg gtactaagtt attatataac aaagagaacc gtttgcaaag ttttttttga
    48901 aaagggatac gattccctgt tgttctttgt ttttctatta agaaactgca gttattttat
    48961 aaccagtatc ctttgatcag taaaggctat tctggtgtac cgattatttt aatgtattca
    49021 tcgatatttt ttatcaacat tttttttatg accaactggt tccgacggtt ccatttgcca
    49081 cttacttcca gcacatattt atttaggggc aaacgtaaga tatattttgc caaatagcga
    49141 tctttaagca ggccattgat cacacggtcc tcaagctgca agttgatccc agtcagcatc
    49201 attggctgtt cttgtaaaac gtacgtatca attaaaagac cttcaaaagt ttccacaagc
    49261 gtcttccttt cttttcaaag taacctgaaa acaggcacta attcttccat tttgatattc
    49321 gttgaagtgg atgcgatcat tctttctttt gtccactgta atctagtata acaaaaaaga
    49381 gcagaagagc caaaaagaaa aaagagcatc gaattctata ggaagtctca ttggcacggc
    49441 ctaaattatt acaaaacatt gattaaaaaa agcccctgca tcgcaggagc atcccctata
    49501 tcgttttaat aggtcaatca aaaagcagta tcaaacacat tttgtatcaa atcaggaaat
    49561 aatagataag ctaagcccag aatcaaaagt accgctagaa gaaaccagcc gattgttcct
    49621 atgaaatact ttaatttttc gaacattgta tcacctcgtt gtcagtatat cacggatcag
    49681 taaattgggg gtgttaagaa tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc
    49741 aaatacttgt aagatacagt aagtgatacc agaagcaatg accggtccgg cggcgattcc
    49801 tcttaagaag acaacaccta aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc
    49861 agaagcaatc aatcctacac ccttggaaga taagacagcc actagcccac cgcaaaaaac
    49921 agctacccag ccaatagggg atttaaaggc ttctaataga tcacgtagac cgatttctcc
    49981 tgttgcgata gggattagaa tagccgctgt gatgatcgtc accccaatat tgattccttt
    50041 gccttgaagc caagggaaaa aggaacggga ggctggtatg agcttcataa tcaaaacaaa
    50101 agctgccgct atcatcagac tttgattttt agctaaaatt gctacaagta aaataattcc
    50161 taaaaaaagc caactttcca tgaaatatat tcctcacttt cttcgaaaca atcataacat
    50221 gtttttagaa agttacaaaa gtaggaattc atccttcaag acctattcat aagctgtttt
    50281 tttcataaaa taaacaaaat cataaaaaaa cactttgctt atttgacaga agacagccgc
    50341 gctttgtata gtggacaaga cttacatttt tttgaagaat cagaaagatc aggtaaaggt
    50401 cttagtaaac gcaagccatt cagaataacc aatattgtgc ttccttcgtg tcctacaact
    50461 ccaagaggta gatttaaaaa ttgcaggaag ttagaagcaa tcaacaagag aataacaaga
    50521 atcgaaaaaa taatattttg tttgatgatc cgccgcattt ttaaagatag ccgatgactg
    50581 tagacaaatt tatttaagtc gcttttcatt aagacgatgt ctgcgatatc catagcggca
    50641 tctgtccctt gccccatagc gacacctaaa gaagcggctg ctaaagcagg cgcatcattg
    50701 actccatcac caaccatcgc atttacttgg taaagttcat tctctttttt gactagttgt
    50761 gttttttctt ccggagtaca gcttgcatag taatgttcaa tccctacttg ttcagcaatg
    50821 gcctttgctg tgccagaatg gtcgccggtt atcatacttg tatggatgtt ttgggattta
    50881 aagtaggaaa ttacttgttg ggtatttgct tttggaatat ctagcagacc taaaacagca
    50941 attaactgct gatctttaga aagataaatc acggttttgc cttgattttc caaacgttcg
    51001 atcatttccg caatctcttt gctgattatc atcgtctcag gatcatatgc gtgcttgcca
    51061 actttccatt gcgcacctaa ataggtagtt tgtaatccaa aaccagtaat ttcttctatt
    51121 ggtaagtatt ggatttcttc aggtattgtg caatcgaaat attgcaaaat cgcatgagca
    51181 agtggatgcg tcgttttttg ttccatagca acaagaagtt gttgtgccaa atccttttca
    51241 tcaaagaaaa agctgtcagt tacaactggt tttcctttag tgagcgttcc tgttttatca
    51301 aaggcaatcg cttttaattc agaaagcttt tctaaatgga taccgccttt gattaaaatc
    51361 ccattttttg cagaatttga aagagcagct agagaagctg gtgtagcaga tgcgacaagc
    51421 gcacaaggag aagccacaac taataatacc attcctcgat aaaagctttc atcccaactc
    51481 caacctagga aaaagtgggg gagaaggatc attagaggaa cagcaagcaa aatcaatttt
    51541 acataattat tttcgattcg ttcgataaat gaagctgttt gagttggcgt attctgcgct
    51601 tcttctacta attggataat cttagcaaag acagtttcgg aactcgtttt tgttactcga
    51661 atcgtgatgg gatttccttg attcagcgtt cctgcataca tctcatcgcc taaatatttt
    51721 tctacaggca cagattcacc gcttaatgca gactcatcga tcgttgcctg tgtgctttct
    51781 aaaaagccat ctatcggaac agctgctcct ttagcgacaa ataccaagtt atccaccttt
    51841 agttgagaaa catctacttc ttgcatttgt ccattttctt ttaataattg ggctttttgt
    51901 ggttgaagat tcatcaaagc agtaatttct ttttgacttt tattagttgt gtattcctct
    51961 aatgcaccgc ttaaacaaaa gatgaatgtc aacattgctc cttcaaagta attaccgatc
    52021 aaacaggcac caatagcagc taaagccatc aataaatcca cattcagtgt ccgatcattc
    52081 cacaattctt tcaagccttc cgatgtttgt ttatatccac cgatgataat ggcactgata
    52141 taaaaaagtg gggtgatggt aagagagaat cggtcgaaga taaaaccgaa gatcataaat
    52201 aaaaggcagc cgattgttgc tgcaaaagct tttttctgat tagtcatcgt tattagacct
    52261 ccttgtcaaa acaaggatag cataataaaa atgtaattga taataataac agctaaatga
    52321 gaacgataat ggttatcatt tggtgattaa gcttgatgtt gtttttatta tttataatga
    52381 ttattattaa agaaaataaa aaaccagaac atttctcaaa taagagaagt gttctggttt
    52441 tttctacatt gcaataaacc aataaccaat caaaatgata ccaaggataa tacgatacca
    52501 gccaaaagcg gtaaagtcgt ttttcttcaa atagcttagt aagaatttga tagctaagac
    52561 ggaaaccaca aaagatacga atgttcctac aagaagaaca acagtttctt ccattttgaa
    52621 tgaattccct tcagctaaat atttgacgat cttcaagccg cttgcgccaa acatgacagg
    52681 aatccctaag aaaaatgaaa actctgtagc gacaaaacgc gaagcaccaa tcaagataga
    52741 tcctaggatc gttgcgccag aacgggatgt tccgggaaca agtgccaaga cttggaataa
    52801 cccaacaaga agagctgctt ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt
    52861 gtttttgttg cgtttttcga caataataaa ggcaataccg taaacgatca aaacaatggc
    52921 aactggtaag aacttgtgga aatttgcttc taaccaatca tccaaaggca aaccaatgat
    52981 gatggctggt atgcaagcaa cgatcacttt aaaccaaagt gtccacgtat cttttttttc
    53041 taccgttgat ttacttggag aaaatgggtt taacttgtga aaataaagaa cgacgacagc
    53101 caagatcgcc cctaattgga taacgacatt gaacatcgac ataaattgtg aactggcatc
    53161 cagtttgatg aattcatctg ctaggatcaa atgaccagta ctactgatgg gcagccattc
    53221 cgttattcct tcaataatac cgagaatcac tgctttgata atatttgcaa aaaacataaa
    53281 ttccttcctt cccaattaag atacatctta tagtataccg ttattttttg agaaacccaa
    53341 tctgtttttt aatattaaga aaaacttgag aaataaagca gataagtaca cgaagagaaa
    53401 aaagaagaca aagccctcgt tcagactttg ccttttatga ttctctggta aaaattaaaa
    53461 tatccccatt atagaaacga gtcgcgacta tgtgttttcc cgttatcgat ataagacgta
    53521 tcattgatgc cgtggataga aaatcgaccg tttgcataag tgacctttgt tacgctgcca
    53581 tttaaaagcc cagcacgaac aggctgttta ggatcgatca gattaagcaa aaaggcgatc
    53641 gtcaaaccat gagatacgac taaaacattt ccaccaccat ttttttcgca gtgatgggcg
    53701 atatcttcaa atcctgtcca tacacggtct cgcaatgttt cataagtttc cgcccaccca
    53761 gcggtatcta agttgtatac agcattagca atttgttcaa aagaaacttc tgtggtaaac
    53821 atttcatcat atgttttaaa attcaataca cgagggatca caccccacat ttccatgtca
    53881 tatccacctt caagagaccc gaaacaccat tctctaatcc ttttttcaat aaaataagga
    53941 atttcttttc ccttagaatg ttctcctaga ataatacggg cagtttcgat tgcacgacca
    54001 ctgtcactag aataagcaaa tgaaaagtcg acatttttta acccgcgacc taaataatga
    54061 atgccttctt gtccttgctt tgtgaggggt gtatcacacc agccttgtac tcgttcaatt
    54121 gtattgaaca tcgtttttcc gtgtcgtacg atataaagtg tagtttctgt cattttattc
    54181 tcctcctaag tcgttttaac gattgaaaaa aggatttgtc cttttttcct gttcaatcgt
    54241 cgttgcatcg ccatgtcctg gatagactgc aaattcatca ggtaatacaa acagctgggt
    54301 ccgaatacta tgaagtaatt gttctaaatt ccctgtatac agatcggttc taccgatact
    54361 tcctctaaac agggcgtcgc cagaaacgac aaaatcatca aaaataaagc tgacgcttcc
    54421 tatagaatgc cctggggtag ggacgacgcg aaaagacatg tttcctaaag tataagtttt
    54481 catttcaaat tcatattctg caggcgagac aataacatca gcaatatcat catgtctacc
    54541 gagtccagaa agattcaaaa tcggatcacc tagccatttt tgctctagtg ggctgacata
    54601 aactgggatt tgataatacg cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt
    54661 atgggtaagc aaaatcgcta ccggctgttg gtttgttttt ttgatttctt gtttgattcg
    54721 ttccgcatct tctccagggt caatgatcaa aagtgcttct tcgttgtaga ccaaataaca
    54781 gttttcttca atcattcctg tttttaatct ttcgatatgc atgtgcattc ctccttgata
    54841 gccaaaactt ctttcatcca ttagtataac gcattcattg ctaggaaaca aaaacaggta
    54901 cttatttctc tttacttatc tgaattatca gactatatga aaagtatttg gatattctaa
    54961 agagaaggaa agtaagtttt catagcattt taatatgtcc ttgttacatt agattcatcc
    55021 aaaaatccaa attctatatc ctatatccct gcctaatcag cagggtgttt ttttatgttt
    55081 ggaaagagag ctctttttgc taaactaggt tttttcaggt aatcttagag agaaagaggt
    55141 ggtggaaatg atcaaacagt caaaaatatc tgtgcggcac ttagtagaat ttattttgcg
    55201 acgtggaagt atcgacaatc gtaaaaaaag taaccatact gcattagaag gagcgagaat
    55261 ccaccgcaag ctccaaaaag atgcgggaga aaactatgaa aaagaagtct ttttaaaaac
    55321 aacagttcaa ttagatgaat attacctgat cgttgaaggc cgtgcagacg gtattttcaa
    55381 aagagacggt atttattata tagatgaaat caaaacctcc gaaccacgat ttgaagattt
    55441 agaaccagaa caagtggaac ttttctttca tcaagcaagg gtgtatgcct atatctatag
    55501 tcaagaacat gatttacaag aaataaatct gcaattgact tacttccaga cggcagaaga
    55561 agtgatcacg agaaaggttg aacatcagac aagagagcag ttagaaactt tttttaaaaa
    55621 gctgactgaa gattataaag aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa
    55681 tgcctctttg atggctttga aattccccca tgatgagtat aggaaaggtc agcgtgaact
    55741 agcggtagct gcttataaaa cgatccgtac gaaacagaaa ttgtttgtag aagcacctac
    55801 cggaacagga aaaacaatct ctacgttatt ccccgctttg aaagctgtag gggaagaaga
    55861 gggagaaaag atcttttatc taacggctaa aacaataact agacaagtag ctgaagatgc
    55921 tatgacagca ttaaaagata caggagctga agtaaaaagt gtcactttga cggcgaaaga
    55981 caagatctgc tttttagatg agacgacctg taatccagat cagtgcccat atgccaatgg
    56041 ttattacaat cggattaatg aaggtttatg ggatcttttg aatcatgaga atcagatcac
    56101 tcgtgaagtc atcgaaactt atgcaagaaa gcatacactt tgtcctttcg aactatcatt
    56161 agatgtcagt atctggtgtg acgtaatcat tggggattac aattatttat ttgatccaac
    56221 tgtttattta agacgttttt ttgaagatga aaaaaacgag gactatctgt ttttgattga
    56281 cgaagcacat aatttggtga atcgttcccg agagatgtat tcggcagaac tatcttatga
    56341 aaaaacgaaa cgaagtaagg aagctatacc aaaggaattc aaaaaacttc atcgccgttt
    56401 caacaaacta ttaaaagaat ttgattcgat ccgagaaata gcaaaagaag atcattggga
    56461 ttatcatcat caaaaagcac cggctgaatc tttagtaaag gcaggttatc aattaagcga
    56521 aaaaattaaa gaatggctgg cagaatttcc tgagcaccca tcacaagaac aattgcttcc
    56581 ttattatttt gatttgcttc attttttgaa ggtcagtgaa ttttatgatg atcactacga
    56641 aacaacagtc gaaaaaacgt atcgcgatct gatagtaaaa gagttttgta ttgatccaag
    56701 cttgtttctt gagcagtcgt tggataaagg aaaaagcagt attctttttt ctgctagttt
    56761 ttcaccactc tcttattatc aggaaacact ggggggacaa aaaagtctgg cttacaaact
    56821 gccgagtcct ttcccagaag gaaaccagca agttttcatc gcaaattacc tagagacgac
    56881 ttatcgaaaa agggaacaca gtctggcaaa attagtagag acgattcatg cttttgctga
    56941 tggaaaggtc gggaattatt tcgttttctt tccttcttat caatatttag atcttgcggt
    57001 ggaagcattc agacagcaat atcccgacag caatgtgcta atccaagaaa cggacatgaa
    57061 tgaggaagaa cgcgaacgat ttttaacaaa atttcaaaca aatcccgctg aaacattgat
    57121 tggattttgc gtacttggcg ggattttttc agaaggaatc gatttgagag gggaccgatt
    57181 gatcggcagt atgatcgtag gtgtaggttt gccgcagatg aatcatgagc aggaactgat
    57241 caaatcatat tatgatgaga aggaacacct tggatttgct tacgcttatc agcttcctgg
    57301 aatgaacaaa gtcttgcaag ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat
    57361 cgttgtattg gcagatcaac gattttctag tttttcttat cgacgtttat tcccacaaca
    57421 ttggcaaaca gcaagagaag tacgaagtgt tggggaattg gaagaaggaa tacagcgttt
    57481 ttggttatca aaagaagcag taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt
    57541 ttttgattaa cgatagttag tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa
    57601 agcaatcaca gcttgtagat catcacggtt cttacctgtc actctaatct gatcttcttg
    57661 gatttgtgct tttactttca gtttttcatt tttgatagct gtagtgattt tcttcgcgtt
    57721 ttcacgatcg attccgttga ccaaatcggc agtttgtcga gctttgccac ctagagcgtg
    57781 ttctgattcg gaaaaatgga tatttttgat tggcacattt cttttgatca gcttaccaaa
    57841 taatacatct ttgatctgtt cgattttgaa atcatcatcg ctcaatacaa ctagattgct
    57901 gttctctaat ttgatttcta ctgtagaatc cttgaagtca aagcgattgg acaattcttt
    57961 tgttgtttgc tgaatcgcat tttttacttc ttctgtattg acttctgata caatgtcgaa
    58021 gctagcatct tttgccatag gaaccctcct gatatacata taaatattta ccatacatct
    58081 atcttatcat aggattaggg cagattctta cctgtttgct aaaattcttc tcatttgagt
    58141 tcgaagtatt ttattttaat agataaactg ttattcttac atttggaata ccaaagtagg
    58201 ggagaggtgc ttgtgagtaa aattatgact gcgtatcata aaaatgaaag attaaagaaa
    58261 atagtggtta ttttgataac cggattgacc gctgcggtcg ctttaaatta ttttttgatt
    58321 ccagccaatg tcttttctgc agggatgaat gggatcgctc aaatcatttc ctcgctttta
    58381 tatgaatggt ttcatatcaa gatagacaca ggaacattta tttttctttt gaatatccct
    58441 gtgtttgtat tagggtttct caaagtagga aaacgagcaa cgatccttag ttttgtgaat
    58501 gttgtatgtg tatcggtgat gacgatgctg cttccagtcg gacagatcac tgataatatt
    58561 ttgatgaatg ccctagtagg cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa
    58621 atgggcttta caactggtgg gatggatatc atctctcttg tactttcaca aacaactgga
    58681 aaaacagtcg gaaactacat gctgattcta aatggtatca ttgtagtcat cgcaggcttc
    58741 ttctttaact gggaaagtgc cttgtatacg attatttcca tttatgcaat gactcaagta
    58801 attgatgcca tccacacgag tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca
    58861 gaagaggtaa cagaagaact agcaaaacgg atggtacgtg gaatgacatt actgccgtct
    58921 gtcggtggct attcaaaaaa acaagggaac atgatcatga tggtcatcac ccgctacgaa
    58981 atgtatgatc ttgatcagat cgttcatgaa atcgacgacg atgcatttat caatatttta
    59041 ccaactcatt cagtatttgg ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca
    59101 actggcgtat tgccagaaat aaaaaatcgt tcaca
//
LOCUS       DAZBSA010000004        55180 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000004 DAZBSA010000000
VERSION     DAZBSA010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.004"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..371
                     /locus_tag="ACHGMT_000217"
     CDS             3..371
                     /locus_tag="ACHGMT_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFN6833996.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            551..1492
                     /locus_tag="ACHGMT_000218"
     CDS             551..1492
                     /locus_tag="ACHGMT_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFN6833997.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1561..2292
                     /locus_tag="ACHGMT_000219"
     CDS             1561..2292
                     /locus_tag="ACHGMT_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFN6833998.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2436..4616
                     /locus_tag="ACHGMT_000220"
     CDS             2436..4616
                     /locus_tag="ACHGMT_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFN6833999.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4636..5118
                     /locus_tag="ACHGMT_000221"
     CDS             4636..5118
                     /locus_tag="ACHGMT_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFN6834000.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5134..5895
                     /locus_tag="ACHGMT_000222"
     CDS             5134..5895
                     /locus_tag="ACHGMT_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFN6834001.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6026..7360
                     /gene="murC"
                     /locus_tag="ACHGMT_000223"
     CDS             6026..7360
                     /gene="murC"
                     /locus_tag="ACHGMT_000223"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFN6834002.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7667..8152
                     /locus_tag="ACHGMT_000224"
     CDS             7667..8152
                     /locus_tag="ACHGMT_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFN6834003.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8145..8828
                     /locus_tag="ACHGMT_000225"
     CDS             8145..8828
                     /locus_tag="ACHGMT_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFN6834004.1"
                     /translation="MNNPSTLMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8954..11599
                     /gene="polA"
                     /locus_tag="ACHGMT_000226"
     CDS             8954..11599
                     /gene="polA"
                     /locus_tag="ACHGMT_000226"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFN6834005.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11644..12480
                     /gene="mutM"
                     /locus_tag="ACHGMT_000227"
     CDS             11644..12480
                     /gene="mutM"
                     /locus_tag="ACHGMT_000227"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFN6834006.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12444..13073
                     /gene="coaE"
                     /locus_tag="ACHGMT_000228"
     CDS             12444..13073
                     /gene="coaE"
                     /locus_tag="ACHGMT_000228"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFN6834007.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            13125..13457
                     /locus_tag="ACHGMT_000229"
     CDS             13125..13457
                     /locus_tag="ACHGMT_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFN6834008.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13769..14260
                     /gene="nrdR"
                     /locus_tag="ACHGMT_000230"
     CDS             13769..14260
                     /gene="nrdR"
                     /locus_tag="ACHGMT_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFN6834009.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14283..15656
                     /locus_tag="ACHGMT_000231"
     CDS             14283..15656
                     /locus_tag="ACHGMT_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFN6834010.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15656..16582
                     /gene="dnaI"
                     /locus_tag="ACHGMT_000232"
     CDS             15656..16582
                     /gene="dnaI"
                     /locus_tag="ACHGMT_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFN6834011.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16711..17151
                     /locus_tag="ACHGMT_000233"
     CDS             16711..17151
                     /locus_tag="ACHGMT_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFN6834012.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17295..17996
                     /locus_tag="ACHGMT_000234"
     CDS             17295..17996
                     /locus_tag="ACHGMT_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFN6834013.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17993..19114
                     /locus_tag="ACHGMT_000235"
     CDS             17993..19114
                     /locus_tag="ACHGMT_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFN6834014.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19129..20361
                     /gene="pepT"
                     /locus_tag="ACHGMT_000236"
     CDS             19129..20361
                     /gene="pepT"
                     /locus_tag="ACHGMT_000236"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFN6834015.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20493..21401
                     /locus_tag="ACHGMT_000237"
     CDS             20493..21401
                     /locus_tag="ACHGMT_000237"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFN6834016.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21436..21708
                     /locus_tag="ACHGMT_000238"
     CDS             21436..21708
                     /locus_tag="ACHGMT_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFN6834017.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21719..22765
                     /locus_tag="ACHGMT_000239"
     CDS             21719..22765
                     /locus_tag="ACHGMT_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFN6834018.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            23016..25625
                     /gene="clpB"
                     /locus_tag="ACHGMT_000240"
     CDS             23016..25625
                     /gene="clpB"
                     /locus_tag="ACHGMT_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFN6834019.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(25776..26462)
                     /locus_tag="ACHGMT_000241"
     CDS             complement(25776..26462)
                     /locus_tag="ACHGMT_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834020.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(26440..26934)
                     /locus_tag="ACHGMT_000242"
     CDS             complement(26440..26934)
                     /locus_tag="ACHGMT_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFN6834021.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(26954..28174)
                     /locus_tag="ACHGMT_000243"
     CDS             complement(26954..28174)
                     /locus_tag="ACHGMT_000243"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6834022.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28321..30156
                     /gene="lepA"
                     /locus_tag="ACHGMT_000244"
     CDS             28321..30156
                     /gene="lepA"
                     /locus_tag="ACHGMT_000244"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFN6834023.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            30352..31263
                     /locus_tag="ACHGMT_000245"
     CDS             30352..31263
                     /locus_tag="ACHGMT_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6834024.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            32274..32510
                     /locus_tag="ACHGMT_000246"
     CDS             32274..32510
                     /locus_tag="ACHGMT_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834025.1"
                     /translation="MFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTELKDKN
                     NVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            32623..32865
                     /locus_tag="ACHGMT_000247"
                     /pseudo
     CDS             32623..32865
                     /locus_tag="ACHGMT_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            32955..33194
                     /locus_tag="ACHGMT_000248"
     CDS             32955..33194
                     /locus_tag="ACHGMT_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834026.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            33341..37624
                     /locus_tag="ACHGMT_000249"
     CDS             33341..37624
                     /locus_tag="ACHGMT_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFN6834027.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            37723..38088
                     /locus_tag="ACHGMT_000250"
     CDS             37723..38088
                     /locus_tag="ACHGMT_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834028.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            38090..38542
                     /locus_tag="ACHGMT_000251"
     CDS             38090..38542
                     /locus_tag="ACHGMT_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834029.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            38991..40466
                     /locus_tag="ACHGMT_000252"
     CDS             38991..40466
                     /locus_tag="ACHGMT_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFN6834030.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            40489..40800
                     /locus_tag="ACHGMT_000253"
     CDS             40489..40800
                     /locus_tag="ACHGMT_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834031.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            41160..42416
                     /locus_tag="ACHGMT_000254"
     CDS             41160..42416
                     /locus_tag="ACHGMT_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFN6834032.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            42413..42958
                     /locus_tag="ACHGMT_000255"
     CDS             42413..42958
                     /locus_tag="ACHGMT_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834033.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            42959..43459
                     /locus_tag="ACHGMT_000256"
     CDS             42959..43459
                     /locus_tag="ACHGMT_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834034.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            43818..44096
                     /locus_tag="ACHGMT_000257"
     CDS             43818..44096
                     /locus_tag="ACHGMT_000257"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834035.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVLQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            44116..44460
                     /locus_tag="ACHGMT_000258"
     CDS             44116..44460
                     /locus_tag="ACHGMT_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834036.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            44479..45390
                     /locus_tag="ACHGMT_000259"
     CDS             44479..45390
                     /locus_tag="ACHGMT_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFN6834037.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVSAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            45469..46461
                     /locus_tag="ACHGMT_000260"
     CDS             45469..46461
                     /locus_tag="ACHGMT_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFN6834038.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            46458..46676
                     /locus_tag="ACHGMT_000261"
     CDS             46458..46676
                     /locus_tag="ACHGMT_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFN6834039.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            46689..47099
                     /locus_tag="ACHGMT_000262"
     CDS             46689..47099
                     /locus_tag="ACHGMT_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFN6834040.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            47151..49679
                     /locus_tag="ACHGMT_000263"
     CDS             47151..49679
                     /locus_tag="ACHGMT_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN6834041.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            49695..51905
                     /locus_tag="ACHGMT_000264"
     CDS             49695..51905
                     /locus_tag="ACHGMT_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFN6834042.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            51913..53004
                     /locus_tag="ACHGMT_000265"
     CDS             51913..53004
                     /locus_tag="ACHGMT_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFN6834043.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            53025..53567
                     /locus_tag="ACHGMT_000266"
     CDS             53025..53567
                     /locus_tag="ACHGMT_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFN6834044.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VGQEAQKIDAIKIYYVRSAY"
     gene            53762..54019
                     /locus_tag="ACHGMT_000267"
     CDS             53762..54019
                     /locus_tag="ACHGMT_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003657401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834045.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            54033..54365
                     /locus_tag="ACHGMT_000268"
     CDS             54033..54365
                     /locus_tag="ACHGMT_000268"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834046.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            54410..54958
                     /locus_tag="ACHGMT_000269"
     CDS             54410..54958
                     /locus_tag="ACHGMT_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFN6834047.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            54965..>55180
                     /locus_tag="ACHGMT_000270"
     CDS             54965..>55180
                     /locus_tag="ACHGMT_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834048.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKIT"
BASE COUNT        18753 a         8985 c        11882 g        15560 t
ORIGIN      
        1 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
       61 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      121 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      181 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      241 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      301 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      361 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      421 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      481 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      541 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      601 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
      661 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
      721 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
      781 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
      841 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
      901 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
      961 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1021 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1081 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1141 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1201 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1261 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1321 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1381 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1441 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1501 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1561 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1621 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     1681 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     1741 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     1801 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     1861 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     1921 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     1981 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2041 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2101 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2161 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2221 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2281 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2341 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2401 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2461 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2521 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2581 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     2641 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     2701 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     2761 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     2821 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     2881 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     2941 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3001 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3061 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3121 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3181 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3241 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3301 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3361 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3421 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3481 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3541 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3601 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     3661 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     3721 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     3781 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     3841 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     3901 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     3961 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4021 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4081 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4141 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4201 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4261 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4321 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4381 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4441 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4501 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4561 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4621 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     4681 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     4741 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     4801 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     4861 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     4921 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     4981 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5041 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5101 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5161 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5221 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5281 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5341 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5401 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5461 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5521 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5581 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     5641 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     5701 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     5761 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     5821 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     5881 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     5941 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6001 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6061 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6121 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6181 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6241 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6301 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6361 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6421 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6481 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6541 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6601 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     6661 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     6721 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     6781 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     6841 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     6901 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     6961 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7021 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7081 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7141 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7201 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7261 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7321 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7381 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7441 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7501 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7561 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7621 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     7681 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     7741 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     7801 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     7861 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     7921 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     7981 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8041 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8101 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8161 cattaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8221 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8281 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8341 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8401 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8461 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8521 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8581 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     8641 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     8701 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     8761 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     8821 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     8881 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     8941 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9001 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9061 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9121 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9181 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9241 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9301 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9361 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9421 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9481 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9541 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9601 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
     9661 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
     9721 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
     9781 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
     9841 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
     9901 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
     9961 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10021 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10081 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10141 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10201 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10261 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10321 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10381 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10441 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10501 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10561 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10621 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    10681 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    10741 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    10801 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    10861 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    10921 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    10981 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11041 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11101 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11161 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11221 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11281 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11341 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11401 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11461 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11521 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11581 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    11641 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    11701 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    11761 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    11821 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    11881 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    11941 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12001 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12061 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12121 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12181 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12241 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12301 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12361 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12421 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12481 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12541 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12601 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    12661 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    12721 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    12781 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    12841 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    12901 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    12961 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13021 aaatacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13081 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13141 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13201 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13261 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13321 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13381 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13441 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13501 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13561 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13621 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    13681 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    13741 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    13801 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    13861 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    13921 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    13981 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14041 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14101 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14161 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14221 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14281 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14341 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14401 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14461 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14521 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14581 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    14641 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    14701 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    14761 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    14821 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    14881 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    14941 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15001 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15061 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15121 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15181 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15241 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15301 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15361 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15421 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15481 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15541 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15601 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    15661 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    15721 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    15781 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    15841 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    15901 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    15961 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16021 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16081 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16141 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16201 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16261 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16321 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16381 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16441 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16501 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16561 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16621 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    16681 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    16741 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    16801 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    16861 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    16921 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    16981 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17041 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17101 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17161 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17221 tatgggaatt tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta
    17281 gaaggagtga agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact
    17341 ttgtccctga atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg
    17401 cattgatatt aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt
    17461 atgaatcagc acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc
    17521 ttgcaaatgg tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg
    17581 gtatgggagg ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa
    17641 gcggacaaga acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc
    17701 tgcaagaaaa tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat
    17761 atgaaatcat tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga
    17821 tgttcgggcc atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag
    17881 agcttcaaca aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac
    17941 gtcaaaaaca aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat
    18001 cagcggacgc actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga
    18061 aggagaccca gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat
    18121 gacattagac gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct
    18181 gattgctaaa catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca
    18241 agaaaaaatg tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa
    18301 catggatatt atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt
    18361 ggagagttat ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc
    18421 tgtggatcat gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg
    18481 tgattatacc ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg
    18541 tgcacaacca gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga
    18601 ggttattttg ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc
    18661 ttatgaggaa cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt
    18721 aggacgagta ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa
    18781 agaagcgttc caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt
    18841 gaagcgcatt gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca
    18901 aagagcagat gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag
    18961 cgctgggttg attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt
    19021 tatcgagaaa tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc
    19081 tgaaacaaat acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat
    19141 cttttgccac gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa
    19201 acgactccat cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa
    19261 gagttaggct tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta
    19321 ccaagcaatg ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca
    19381 gattttaatg cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg
    19441 atcgtactag atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa
    19501 aattatgaag gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac
    19561 aaatcaggga ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc
    19621 cctcacggta ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat
    19681 aaattcgatg ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta
    19741 ggtgagttgc agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa
    19801 aatgtacatc cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat
    19861 tttcataatc agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc
    19921 ttccatttga tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc
    19981 gatcatgatc gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag
    20041 ttgaatcagc gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac
    20101 atgaaagaaa tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc
    20161 gagcttggta tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc
    20221 tcttacatgg gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga
    20281 ttcgaatttg tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca
    20341 gaattgaaca caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca
    20401 atgatggaat gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc
    20461 ctcttttttg aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca
    20521 aaagttcagg gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag
    20581 aaaagcagcc agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg
    20641 aattagtcaa gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg
    20701 gcacgattca tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat
    20761 tacttccagg tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag
    20821 aagaagctgc tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca
    20881 atgatgaagt cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga
    20941 tcagtcaaga agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt
    21001 tattgctgaa gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag
    21061 gaaaatccca actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg
    21121 acgactcagc gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata
    21181 ttttccgatt tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg
    21241 gcattactta tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca
    21301 ctcgaacgga tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag
    21361 gattaacagt ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa
    21421 gttggaagaa gagtaatgga tgtattgtta cacaatcctt tgtggtttta ccttttcatt
    21481 gtgaattttt acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc
    21541 tggcgtgttc ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga
    21601 ttgatctctc gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc
    21661 gctgcaggtg tacttgttga tttgattctc atttattttt tccattaagg agcaggagat
    21721 gtcgtctaaa ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt
    21781 tgccattatt tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc
    21841 taccagtgtt gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga
    21901 agggcgctca atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg
    21961 gttcttgacc tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct
    22021 gatctcggag atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt
    22081 gacagcactc catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt
    22141 ggtcatccaa ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc
    22201 tggtatcctt ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa
    22261 tgttcaaata ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa
    22321 atgggtggat acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat
    22381 ccaagacagg actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg
    22441 gtatgtactt ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac
    22501 catttcgttt atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat
    22561 tggttccttt gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc
    22621 agtttcttcg cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt
    22681 tgtgtatgtg attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa
    22741 agagaataaa gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa
    22801 agaaaaacgt tatggataag ataattttac gtttgtttgg ctcttttttt atatacatac
    22861 aatcaatttt gtttttttaa gatttatata agtcgagaaa aaaagtcaaa aaactatgtg
    22921 tatattttga ccaaaattga ccaaagcgat ataatagttt ttgtaaagat caaagaaata
    22981 agtgatctta cctttaatta ggagggtata cctgtatgaa tattgagaaa atgactacga
    23041 cgcttcaaga agcgattgct gaagctcaac agattgcggt cactcgaaaa catcaagata
    23101 tcgacattgc ccatgtatgg aaaatctttt tacaatctaa tcattttggt cgaaatttct
    23161 atacggatgc cggattggat gtcgaatctt ttgaacatga aattgatcgt ctactggatg
    23221 agtatccagt tgtttctggt ggaaacgtcc aatatggaca aaacctcagc cagaatttct
    23281 ttcgattatt gaatgaagca gaccaaatac gtgaaagctt cggcgacgag tttctttcaa
    23341 cggaagtcgt tattttagcg ttgatgaaat taaaaaatta tccgttaact gtttatttga
    23401 ataaaaacgg attatctgaa aaagaattgc gtaaaaacat cgaagagatg agaggaggag
    23461 aaagagtgac ctcacaaaac caagaagaac aatacaaagc attagaaaaa tacggcgttg
    23521 atttagtaca gcaagtcaaa agcggaaaaa tggatccaat catcggtcgt gatgaagaaa
    23581 tccgtgatgt catccgtatt ctttcacgaa aaacaaaaaa taatcctgtt ctgataggag
    23641 aacccggtgt agggaaaacg gcaatcgttg aagggcttgc tcaacgtatc gtacgcaaag
    23701 atgtgcctga aaatttaaaa gataaaacga ttttttcatt agatatgggt gcattgatcg
    23761 ctggagcaaa attccgtggt gaatttgaag aaagactaaa agcagtcttg aaagaagtca
    23821 aaaaaagtga tggacgtatc ctcttgttca tcgatgaaat ccacaatatc gtaggggcag
    23881 gaaaaacaga aggaagtatg gatgccggaa atctgttgaa accaatgctt gctcgcggtg
    23941 aattacattt gatcggtgcc acaactttag atgagtaccg tcaatatatg gaaaaagaca
    24001 aagcgttgga acgtcgcttt caaaaggtat tagtcaaaga accaacagtg gaagatacga
    24061 tcagtattct gcgtggatta aaagaacgtt ttgagatcca ccatggtgtt aacatccatg
    24121 acaacgcatt agtagcagca gctactttat ctgaccgtta tattactgat cggttcttac
    24181 cggacaaagc aatcgatttg atcgatgaag ccagtgcaac gattcgggta gaaatgaact
    24241 ctatgccaac ggaattagat caagttacta gacgtttgat gcagttggaa atcgaagaag
    24301 ctgcactgaa aaaagaatca gatgatgcaa gtaaaaaacg cttgaaaaat ttgcaagaag
    24361 aattagctga acttcgtgaa gaagcaaatg cgatgaagat gcagtgggaa acagaaaaag
    24421 aagaagtcaa ttctgtttca gcaaaacgtg ctgaaatcga taaagcaaaa cacgaattag
    24481 aagatgcaga aaataactat gatttagaac gcgctgctgt attgcgtcat ggtacgatac
    24541 ctcaattaga aaaagaattg aaggaattag aagctaaagc aaaagacagc gagattaaga
    24601 tggttcaaga gtccgttacc gaaaatgaga ttgctcaagt cgttggcaga ttaacgggta
    24661 tccccgtaac gaaacttgta gaaggtgaac gagaaaaact aattaaattg aatgaaacac
    24721 ttcataaacg agtgattggt caagatgaag cagttgatgc ggtcagtgac gcggtaattc
    24781 gttcacgggc tggattgcaa gacccaaatc gtcctctagg ttctttcctt ttcctaggac
    24841 caactggtgt cggaaaaacc gaattagcga aagcgttagc tgagaacttg tttgactcag
    24901 aagatcatat ggtacggatc gatatgagtg aatatatgga aaaacatgct gtgtctcgtc
    24961 tagtcggagc tcctccaggc tatgtaggtt acgaagaagg tggtcaatta acggaagctg
    25021 tcagacgaaa tccttataca atcgtcttgt tggatgaaat cgaaaaagcg catcccgatg
    25081 tcttcaatat cttgttacaa gtcctagatg acggacgctt gactgattca aaagggcgag
    25141 tagtagattt caaaaatacg gttctgatca tgaccagcaa tatcggttca cagttactgc
    25201 tggaaggcgt gactgctgat ggaacgattc cagaagcagt ggctgaacaa gtcaacacgc
    25261 tattacgtgg aaacttcaaa ccagaattct tgaaccgaat cgacgacacg attttattta
    25321 caccattgag cctagacaac gtgaaaggaa tcgttgacaa aatggtggcg caattggcac
    25381 aacgtctgga acatcaagaa atcctgctga caatcagtga tgaagccaaa acgtggattg
    25441 ctgaaaatgc ttatgaacca gcatatggtg caagaccatt gaaacgattt atcactaagg
    25501 aagtagaaac accactagct aaagaaatcg ttgcaggaca tgttatgcct aaatcaaaag
    25561 taacgattac tttacttgat ggacaacttc atttcaaaac agaagagcta gaagaaatcg
    25621 tctaatcttc cattgagact ataaaagaaa aaaggggtct gacacaacta gtgtcagacc
    25681 tccttttttc aaataaacag tgttccagaa gtagtttctt cgaaaaataa gccgaaattt
    25741 cttgaagctg cccgcttctg tcacaacctc ttttattagg tatttttcac tttttctaat
    25801 ttataacttt caaagtaacg ctcatctttt ttactttgag accatgcaaa caatcccatt
    25861 aaggcagcac ctatcgtatt tgtcagcaag tcgcccatcg tatccatcaa tgctgctcgt
    25921 ccgacaaaca gtgtgccatc agaagcagca aaacgttgaa gattcatgcc taagaactgg
    25981 tcgcataaga attcccagaa ctcccaaaac acgccgcaaa gtccagcaaa cgcaaagccg
    26041 aataagagga acaaccaagg actggttttt gatatctcag catctttcat caagtaacca
    26101 atcaatccat agccaagagc tgtcaggacc attggactca ccgcatgtaa gattttgtcc
    26161 caaaaagaga tgatactgat caaatgcatc cctgtgccta aaaagacaga gatgaacaag
    26221 aaaaaccagt aaaacaaaac gattgctgct ggtattttaa gtttgaaaac tttcaaaatg
    26281 acttgtggca tgaaaatcaa tgcaatccct aataaagatt ccacgataaa tgtgattcct
    26341 tgagaagtcg aatatttttg tgtgataaat tcataaaaat tatagcagac aacgaataca
    26401 ccaaaagcaa gtaaagctat ttgcaccttt ttgatttctt taatcactga aagtccctcc
    26461 atctaaaaat ctttttactt ctgaaagcaa ctcaatttct gaagaaacca cttttccgtt
    26521 caatttgatc aaacctattg tatataagtt caggtagtga aattgatttt cagctgtttc
    26581 ttgtagtgca gcgatttttt tcgggtgggt tccgccttgc tgtcgagtat cagtatataa
    26641 gccgatcact gggatgcctt tcgcataagc cacaccgatt tccgatgcta ccccggcatc
    26701 gatcgtcagc ccatctagta aagcaatcat taaatcacta gccaacactt tttctgtgtc
    26761 agcaagagca atcattcgac tgtctgcata agcagttttg tcattgattg cttcattttc
    26821 ttgaggaaga tagatcgaaa gctcagaaga gagctctctg atttctttaa ctaactgttg
    26881 gttatataac aaatcacttt tagcaaacat tggtgcggca aaataaattt tcatctaaac
    26941 atcatccttt ttatcaaaaa taatcgctaa agtaagcttc catgtttgga atgatacgct
    27001 ctagtgactt cagtgtttct tcatctgttt ctaaccgttc gattcttgcc aaataagaag
    27061 cacggcgata attcatcatc aagcaagtca atgtttggat atccagtcga acggattttc
    27121 ctataggttc atttaagaca cggacttgtc cctgttcatc ccatgttaaa gcgaagacac
    27181 cattattcca aggagcgaca ggatcttcaa tgatgaaatg aaaagcatca gctgttccaa
    27241 caaacggaaa ccgttgaagg aattctttta catcaacgat ccgagccata aaatatggtt
    27301 cgatctgctc tttgatctga ctatcttcaa tcaaaaatgc taaaggctca tttttataaa
    27361 tatctccatg tacccagtag atcatggaaa aatgtgcgga aataaagttc cataagccat
    27421 ttcgagcttc ttgatcaaga tagaacattt cttttacgtg aaatatttct tcagctaccc
    27481 agtagaacag aacacctctt ggttcatggt tgctatcgta gtagacagcg gctgtgcgct
    27541 catcttcatt ttcgaaacgc caatactctt cccaatgaaa atcactgcgg aataaagcac
    27601 catggttttg ttgggcaaat ttgtcataaa cggaaaatac gtctggatga tcaactggta
    27661 atcgctcaac cataccactt actgggattt gtttaggtag ctgtgtatct ctgattttaa
    27721 aggaaagttt atcagacatg atttcccatc ctttacgccg atagtagggg atactgtaag
    27781 gatacaaata agagatccat tgcttattct tgcgcatatt atccaatgca acgataatta
    27841 gatcttgcat caatccatgg ttagcatatt ctggataagt tccaactcca gtcacaccac
    27901 ccattttata acgtgcaccg tgaatattga cttcacaagg atagatagcg atttgagaga
    27961 tcagtttatt ttcgtggaac catccgaaca ctttcgatac ttccaaaatc ggttttttgg
    28021 aacggataaa agctcgttta ttctcaaacc cgctttcctc gatgtctgct tcagtcactt
    28081 ggaacacata ggaaagcaat tcattgaatt gatcgatatg ttcttgagtg accggtttta
    28141 attcaagatt ttctctaaaa tctttttgat ccattttttc atctccattt attagcttga
    28201 taacagtata gcaaaattct acaaaaaaaa cttctatagc tgactaatta aaaactcttt
    28261 aaattactgc ttttcgttgg tataatggaa agtgctgatt atatgaaaag aggagacgct
    28321 atggatataa agaaaatgaa agaacgccaa gagcagattc gtaatttttc gatcatcgct
    28381 catatcgacc atgggaaatc tacattggcc gatcgtattt tagaaatgac gcacactgtg
    28441 acgtctcgcg aaatgcaaga tcagctgtta gactcgatgg atttggaacg tgaacgtgga
    28501 atcacgatta aattaaatgc tgtagaactc aattacacag cgaaaaatgg agaaacatat
    28561 atctttcact tgattgacac cccagggcac gtcgacttca cctatgaagt ctctcgaagc
    28621 cttgctgcct gtgagggtgc cgttttagtg gtcgatgcag cacaaggaat cgaagcacaa
    28681 acattggcca atgtttattt agcgttagac aatgatttgg agatcctgcc agtcatcaat
    28741 aaaatcgacc tacccgcagc agatcccgaa cgtgtccgtc aagaaatcga agacgtaatc
    28801 ggaatcgatg ctagtgaagc tgtattagcc agtgccaaat cagggattgg gattgaagat
    28861 attctcgagc agatcgtgga atatgttcct gcaccaagtg gagacttaga tgcaccgcta
    28921 aaagctttga tctttgactc tatttatgat agttaccgcg gtgttgttct aaatgtccgg
    28981 atcacggacg gtatggtcaa acccggagac aaaatcaaac tgatgagtaa tggcaaaaca
    29041 tttgatgtta cagaggtcgg ggtcttctcg ccaaaagcag ttgctagaga ctttttgatg
    29101 gtcggtgatg tcggttatat cacagcaagt atcaaaactg tccaagatac acgagtaggg
    29161 gatacagtaa cgttagcaga caatcctgcc gctgaagctt taccaggtta ccgaaaaatg
    29221 aatccgatgg tttattgtgg tctttatcca attgatactt ctcgttacaa cgatttgcgg
    29281 gaagctttag aaaaactgca attgaatgat gctgctttac aattcgaacc agaaacctct
    29341 caagcattag gttttgggtt ccgctgtgga ttcctcggac ttcttcatat ggatgtcgtc
    29401 caagaacgct tggaacggga attcaatcta gaattgatca cgactgcacc atccgttatt
    29461 taccatgtta ataaaacgga tggtacgact gctacagtag ataacccggc agattttcct
    29521 gaacctgtaa cgattcagga tgtagaagaa ccattcgtca aagcacagat catggtccca
    29581 aatgattttg tcggtgcagt aatggaactt tctcaacgaa aacggggaga attcattacg
    29641 atggattatt tagatgatta ccgagtaaat gtcgtctata atattccgct ttctgaaatt
    29701 gtctttgact ttttcgacaa attgaaatca agcacgaaag gctatgcctc attggattat
    29761 gaaatgtctg gttatcaaaa aagcaagtta gtgaagatgg acatcttgct gaatggagaa
    29821 aaagtcgatg cattaagctt tatcgttcac cgagactttg cctatgaacg tggaaaagca
    29881 atcgttgaaa aactgaaaaa actaatccca cgccaacaat ttgaggtgcc aatccaagca
    29941 gctattggac aaaaaatcgt tgcccgttca gacatcaaag ccttgcgtaa aaacgtcttg
    30001 gctaaatgtt atggtggaga tgtttctcgt aaacgtaaac tcttagagaa acaaaaagaa
    30061 gggaagaagc ggatgaaaca aatcggctcc gtcgaagttc cgcaagaagc cttcatggcc
    30121 gttctgaaaa tggatgagca agatagtgtg aaataactta taatcaatcg aaaaacctat
    30181 taaataaacc tttttgagta gatgcaacac tgaggattat ctgtataaaa gattttaaac
    30241 tgtcatttgg ttatcacggc gatactaaaa ttaacaaaat tggaatattt ttcttgaaga
    30301 tttttagctg tcagtatatg atgaaagcat ctcataggag gtgctttttt catggttgat
    30361 aattcaagtg gaagggttct taaatctcta agaaaagaga aaaaactatc tcaaaaaaag
    30421 ttagctgatt tagcaggtat ttcacaaagt actttagtta aatatgaaaa agggtcaaga
    30481 aaaatcccta aagacgtaga taatactcta agcaagattc tgaatataga aactctaata
    30541 aaagatgagg aagataagat tgagattctc atcggtaaac taattgcata cagagatatg
    30601 aataagttac ttaacaaaga gttagctgat aatataggaa ttagtgaagt attactcagt
    30661 tatgtgttaa atagaaagag aaatccgtca aaagaaatgc aaaaaaaaat cgatattttt
    30721 ctattaagta atgaaaaaga aattttgaag gagattaatc gtgatagtga gatatttagt
    30781 ttatcaaaag acgataaaat tgttatggga aaaagaatac gagaagtccg aaagaaccgg
    30841 gaagagacgc ttgaaaaatt tggtaaaaac tttaccatct atacaggcaa aaatgttata
    30901 agtagatggg aaaaaggtat taatattcca gatattgaaa aactaatgaa tattgcctat
    30961 cttggtaagg tcactgttcc ttaccttatg tacggagaag attataagaa tatacttcca
    31021 aaagatgaaa gagttagcga ttttaaaaag ataaattctt ttagtatggg attaaggatg
    31081 aggaaaatta ggaaagatta ttatctagaa agggaggagt ttggaaagct tttttctcct
    31141 tcaatttcaa aatggtccat agataggtat gagaatggta gagatatacc caatactaat
    31201 cgaattatcc aatatgctta tataggaaat ctttctttag aatttttgat ttacgggata
    31261 taaaaatagt ttacgtcgat acttattaag ttatcgacgt tttttattat agaaaaaatt
    31321 tataaaagga ggtttcttga tatgataaga aacaaaaagg gtttgttgaa agttgcttta
    31381 ctggcagctt ttttctaagt aactttttga caagctcaat ggcaacacaa gcggaagaag
    31441 taatacaaga actaactgtt atgaataaca gctagataaa atgggctatg tatgttatcc
    31501 aacaatttag taatcactag aagcctagcg aaagttcgct tgataagtta tagatgagta
    31561 ttgatttgta gtgtttccaa atcaaaaggg tacagagcca catgaaatat gttgtcctct
    31621 gcagcgtcag gatgggaatc cataccatgg agattcggag atctgtgagg gaatacttac
    31681 gtggttaata tgtcagttgt gttggacgta gtaatggtca tagtgggtag attgactgaa
    31741 ttcttatatg agaaaatgaa accaacaaga agatagggtt atttatacag aattggggat
    31801 cttttctgga aaagtaatcg gataatgcga gattcgtacg gtctttttta aatttctcta
    31861 aaaaaaacgg catcgaatgg gttgggtgat tgataagcaa tgaatcatag actgatatag
    31921 aataaaaaat gattctattt atctttttac atctatcgtt ttttcaagta gccgcaaatt
    31981 atatcagcat tcaacaattt ttgtatacaa gttctagtaa aaggatctga agaaaaagtt
    32041 ggttgttgtg aagataaaaa aagaatcagg ttttactgag tttttcttca aaatttgtgt
    32101 tattaggtta ggacaaagat aagaagaagt atgaagattt aaaaaaggaa tgaggaaaag
    32161 atagtaattt ccgattattg gagttttttt atttataaag aagaagcgaa taagttgtga
    32221 ttcctagatt tcgcgcgtga ttaattaatg agcaaaaaat gcggaaagtt ttagtgttta
    32281 atacacgagg acaaagtttc ttaggtattg aaggcacacg tgtaacggtt gatgggtggt
    32341 gtgattgttt cgaggttgga aaacatgttg aacttatggc atttactgag ctaaaagata
    32401 agaacaacgt ggaatttttc gagagtgata ttggttggga cgatcatgaa gaagtttatg
    32461 gaaaatttat tttgcggaag ggaaattctt atacgaatgg ggaagcataa gtagcgattt
    32521 gttagagatc attgatagga ttgaaattgt cgggaatatt tatgagaatc tagagttatt
    32581 tgaggtgata gaatgagtga tggttttgaa caagtatcaa taattaaaaa tcaagttcgg
    32641 gagattctta gaaaaaaagg ctatttggta gatagttatt ttgaaggaga ttacgaaaca
    32701 tgggtaggtg tctatgctcg tccagagaat aaacctacct atttagatcc tacgacgtca
    32761 gaagatgcct atttacaaaa taggtatcga gtagatggtt ttaagcaaga ctttgcggag
    32821 tggtttgaat gggaaattga aaacggagaa gtaaaagaag aataagagta cttgttgtat
    32881 caaaataagc cagtagaaca accaaaccag tagctagtaa tgacaataat actgaggaat
    32941 cggatcatta tggtatgatt gatatttctc ttccagaaga tcttaagcca gatcctgtaa
    33001 caattggcaa tagtgtcatg atttttgatt cattagatga agctatgtca tatggttgga
    33061 aagaaattaa ggatgaaggt agtaattggt atggcatgag atttggtgat ggtgaagttg
    33121 ccaaaaaagc tggtattcca attgggaaat ggacagttga tttccacgaa gcaggttctt
    33181 tttttgttct gtaattatag tgggagggta aaatcattga tgaaatgtgt tcagataaga
    33241 gcgccagata aaaatagaag agacagttca cgtcgttatc acttttggta acggcgtttt
    33301 gttttattat ataaaattct caggaaaagg aggaattctt gtgtatgcaa aaacgaaaat
    33361 gaattggttg aaagcactcg cattggtggc tttttctcaa ttctgtttct tggtgggaac
    33421 tgctcaagca catgcagaag aaacgacggc tatacaacct acgatggaag ccattgccca
    33481 agtcgcttct gaagtgacag aagtccaacc agcagaagcc ggggtcccag ctggtgaagc
    33541 cactgttgaa caggcccctg tgacagaagc gccaacggtc aattccaaaa cgactctcga
    33601 tcagacgatt ggggaaacac aacacccagg gaatagtcct ggtacttcac aaccagttga
    33661 agaaactgcc tcaattacaa cccctgcttc agaaacgtcc actctagaag gagaaaaagc
    33721 tgccgaagca acgccttctg tgcagccaga gtcttctgag aaacaagcgg aagaaaagaa
    33781 acgaatagaa gaaacagtgg aacaaccagc ggctactgct gacaaagaag gaaaaaatga
    33841 ggaaaacgtt gaaaaaacaa tctcttcaac cgcagctagt caaaatcaac caacgaaacc
    33901 aaacaaagaa tctacggcta gtattgaaaa tcaaaagaaa aaagagcaga acattccaaa
    33961 aacaggtaca cctacactgt tagtaacagt accaaccaat cgttcggttc gtgttcgccg
    34021 tgcaaccaat gcagctgttt taggtggtcg gaaaattgat tggggcgaaa atattggtgt
    34081 tacttctagt ccgaacaatc taaacttcac tttaaattat atggttcgtt ttgagattaa
    34141 tggacaagtg gcgttttgta ttgagcctgg tgtctcagcc gcagaaggtc aagtttatac
    34201 agctactgct ttgaaaaact atttgaaaaa ttcaaatgta cggaaaaaaa caagcttgat
    34261 ctcttattta ggatatgtca caaataaaga caaatccgat gaacaataca ttgcggcaca
    34321 atttatgatt tgggaagctt tgggcacaaa aatcccaatc aaaaatatcc gtgttgatta
    34381 tgatcgtcga aaagcagaaa ttcaacgttt gattaataac tttggtgaaa aagcaacttt
    34441 taataatcaa acacatacgg taaaaattgg cgaaacgttg aaagtaaaga ataaaaatga
    34501 ttttttgaaa tatgtgaaag acattcaaaa accaaaaggt gtcaatgtat ctattcacgg
    34561 caatgaagta tggattacag tcacaaaaga ggcaccggat aaagcaaaaa ttcaattgaa
    34621 taaaattaaa gttcaaggtg cgccattagc ctatcgtcgt ccaggagctc aaacaattgg
    34681 tgtgttacat ccaacagaac ctaatgcgtc tgttattaat ttacaaattc taaaacaagg
    34741 aaacattcgt gtgtttaaag aagatgcgga aactggtgtg aaagcacaag gtgcagcttc
    34801 tttagaaaat gcggtctatg gtttgtatca agcagatggg aaaaagatta aagaaattac
    34861 cttgaaaaaa attaacggca aagtacaggc agaaattaaa gatcttgatc ctggcaaata
    34921 tgttttaaaa gaaatcaaag cgccaaaagg ttatgtgctt tctgatgaag caattgaaat
    34981 tgttgttgaa cctggaaaaa ctgtcaatgc gaccgcaaaa gatcaagtga tcaaaggaac
    35041 cattgattta gtgaaagttg ctaataaaga tttagtcgat tcaacgaatc cagataacaa
    35101 accaaaatta gctggcattg aaatcacttt gacgtcaaaa acaactggca aagtggtgaa
    35161 aaccgttgtg acagacaatg atggttatgc tacttttggt aaaaataccg ttgtctttga
    35221 tacgtacatt ctttctgaaa ccaaaggaaa agaagggtat aaactatttg aaccacttga
    35281 agtcacaatt tctgaaaatg ggcaaacttt ccattatgtt ttagaagata aagtaattga
    35341 acaacgtctt aaagtggtga aagtggatca agaaacagga aaagtgattc cacttgctaa
    35401 tacagaattc aagatttttg atactttagc caatcgttat gtcacaatgg agattccaaa
    35461 tgatacagaa acaacggata tctttaaaac caatgaaaaa ggctttttcc taactaacgg
    35521 tacacttact tatggaaaag atcgctatcg tttggaagaa atcaaagccc ccgtaaatta
    35581 cgttttgaat caaaacccat tagtgttctc gattaccaat gatacagaac cgatcaaagt
    35641 gatccatttt gcgaaccgtc aagcacggaa aagtgtaaaa ttattcaagt atgaagaatg
    35701 gaacaagaag aaaacgccgc tcgcaggtgt gaactttacc ttgtataacg gtaaaaatca
    35761 agcaattggt gaatatacca ctgatcaaaa cggggaaatt ttagtgaaag gtctagttgc
    35821 cgatgactat tacttcttgg aaaatgcccc attagaaagt ttccaaccaa atacagataa
    35881 acaagcattc acggttgcat tcaaacaaaa tgcgccagat gatggggaaa cgttagtggt
    35941 ttctgtacgt aattacttga ttccaccaaa actagaaaca caagcgtcaa ataaagccga
    36001 tggtgaaaaa gtggttgatc cattagaaac gatcgtcatt aaagacagtg tttcttatac
    36061 caacttgttt gtcggaaaag aatacaccat ttcaggagta ttgatgaaca aggcaacgaa
    36121 tgcgccaatt ttacaagatg gcaaagaaat cacaagtacc ttggccttta ttgcagacaa
    36181 agtcaatggc acaaaagacc ttgaatttgt ggttaatgct agtgttctga aaggtcaaac
    36241 gatcgttgtt tttgaaaaac ttttccgtga tggcattgaa gttgcagcac atgcggatat
    36301 caacgatgaa gcccaaagtg tgaaagtgaa taatccaacc gttcatacca cggctgtctt
    36361 tgaagacgga ttgacagtcg cagacccaac aggattgttt accttaaacg ataccgttga
    36421 aattaccgag attcttccag gaaaagcata cttggtcaaa gggatcttaa tggataaagc
    36481 aaccggtgaa cccttgcttg tgcatggaaa tacggtagaa acgagtgttg gctttattgc
    36541 caaagcagaa aatgaaacgg tgatcgtacc atttaccttt gatttgacag gtataaatgg
    36601 tcgtgaaatc gtcgtgtttg aaagtctttt tagaaatgat gaagaactag caaaccataa
    36661 agacatcaac gacaaagggc aaacggtacg agtaactaac ccaagtgtac gtacaacggc
    36721 tacacataaa cccactggct tgaaagaggt taatccatta acgaaagtca ccattacaga
    36781 cgctgtgaaa taccacgatt tggtcgttgg aaaaacatac acagtcaacg gtgttctaat
    36841 ggacaaagta acggaaaaac cagtgctagt agatggaaaa gaagtcacaa gtaccttaac
    36901 gtttgtggca gaaacaacgg atggtacagt caatctagac tttattctag acgcacgtgc
    36961 gttgcgaggc aaagaagtcg tggtctttga agatctgttt agagaaggcg tattattggc
    37021 ttctcacgca gatattaccg acaatgacca aaccatcaaa atcagtaatc caaccattac
    37081 gacgaaagcg acgaataaaa atggcggaaa agagatcttg gccttgccaa atcaaactgt
    37141 gcttgaatgg gtcaaagtag acggtcttgc gattggtcaa atctatcgct taattgttca
    37201 aggctactta acaccagacg gcacaccatt tgaaggcact catgcggaaa aagtctttgc
    37261 ggccgataaa gaaatgatgg aattcttatt tgaattctta gtggacggtc gcaatcttgc
    37321 aggaaaaggc ttgagttttg cggaatggct ccaagcgaaa acggaagatg agaaagaaac
    37381 gtttgaagaa gtggcgaaac acaatgtaga tctaacaaac aaagatcaaa cggttcaaat
    37441 tgtggaagcg ccaaaaccac cgcaaccagt gaaaccagaa caacgggtta aaccagctac
    37501 tgttgtacca atgttgccaa aaacaggtga acaaacaagt agtatactta tggttcttgg
    37561 attagtagta ttagtcattg ctttaggtat aacactttat aaagtacgta aagccaaaga
    37621 ttagtcatga ggaacaagcg gttaggattt ttaaccgctt gtttgattgt aatgtagaaa
    37681 tatttagaat ttattttggt ttttaagagg aggaaaaact ttatggcaga aaaagcattg
    37741 cgtatgaaga aagagttttt aggtcaagta tttttattgg gttcacggga acctaatcca
    37801 ccaaaggaaa ttaatggtgt gtctgggcga tatgttcaat tacaagcaga gtgttcgcga
    37861 gaagaacagc ctgctttttt tgtttatctc acagctgaag aggataaaag attacgatta
    37921 ccagaaaaag aggtgcgtat ttcatttgaa gaagcttatt ttgtttttcg taaagaacga
    37981 gtgaatagtc gtgaaacaaa tgatatggga caaacagagc gtcgaacaac tggctatcaa
    38041 gatgttggtg tctttttaac tggtgtaaaa attggggagg ttttgtaaga tgggattaaa
    38101 atttccaaat aattatgaca ttacagaagc attagatgaa acttgtttag gagccgtacg
    38161 ctttggcgca ttagaagagt ctgatcgtgt atttgataat gaaacaggcg attatacggg
    38221 agaaattcga tcacagcgag cacaattagt ttttggaaat ccagatgaac caaagattgc
    38281 acgacgagtt aaatttccag cagatgtaga tttgactggt tttaaacgac gtgatcctgt
    38341 gaaattaatt gatccagaat atcatgtggt atatattcca gcacaagatg gcaatcgtga
    38401 atattcagat ttagtgattt atgcgaaagg attagagaaa gtagaagctc caacaaataa
    38461 tcctaatatg aaacaatcac aagaaaaagg tttagatcaa aaacaaccaa gtgttgattc
    38521 taaggaaaaa gagaaaaaat aaggtttttt tgaaagaata ggaaggagtc ttcctattct
    38581 ttttatctaa gatagaagga aggggagatt ttgaattcag ataaaaccaa tcaaatactt
    38641 tgagagattg tgcttgttat caaaatatcc aacaattgat tgagtatttt ggagacaatc
    38701 caacgatttt tgtacaaaca aatgcatata tttgtgcggt tattgggcag tgtatgtttt
    38761 tacgtgatct tgttcgtttt tcattaccgt accagtggaa acaagatcat caggaagtga
    38821 tttggattcg tatagtacga atatgtaata tgtgtgcaca cccttataaa aatcgattaa
    38881 atctttttcg attatgtttt tttactaaat gtgatattcc tgtattagtt gctttttgtc
    38941 aaaagttttt agcacaggaa aagtaaaaac agaaaggagg aatgtgacaa ttgaaagctc
    39001 attttttcaa gtatcgtggt cggcgaattc gctatttcaa tcgttattta tggttttgct
    39061 attatggttt ctttttgttt ccatttttga ttctgttagg ttggtttagt tggaaagtat
    39121 tttatccacg tttaagtacg ttttcgaatt ggcaaatgta tcttattcct ggaataagta
    39181 ttgtgttgta tttgttgctg gttagtgggt taattttagg gttgatgcat tggtcgcgac
    39241 taaaagaagg atattttgcc tctgtttatt ggcgtcagtt acttgttcga atgttaattg
    39301 acaatcggtt tgtttataca aagaaacaag ttagtgagaa acaaacaagg gaaaaaatga
    39361 gattaccaaa agtttatttt tatcaaaaga aacaagaatt agtggtttct tttcctttag
    39421 atggtgggcg ttttcacgaa cgatttttaa aaatgggtca tttgttatct gaagtatttt
    39481 tgcgtgattt aattcgtgaa gaacgagaaa aagcccgaat caattatgta ttcctttcag
    39541 attctggtcg gattcctatt gatcaatgta tagcagaaaa tggacgtatt catttaatga
    39601 aaactttgga ttgggtatat gatcaatcac caaatatgtt gattgctggt gcaatcggtg
    39661 gtgggaaaac ctatttattg tattctttaa ttcaagcttg tttaagtgtt gggacagtag
    39721 atatttgcga tggaaaagcg gcagatttag cagcattagg tgacgtcgat gtattcaaag
    39781 gacatgtgtt ttacaaaata aatgaagaaa tgattatttg tttacgaaat gcattaaaag
    39841 aaatgaatca acggtatgcc tatatgaaaa ccatgaaaga tcctaaatat gaaccaggga
    39901 aaaattatgc ttattatggg ttaccaccgc attttatctt ttttgatgaa tgggcagctt
    39961 tttatggttc gttggatatg gaagcgagaa atagagtcga taaattgatt cagccattag
    40021 tattaaaggg tcgtcaagca gggatgtaca ttattttagc aatgcaacga ccagatgcag
    40081 aatattttcc aagtggtgtt cgggataatt taacattccg tatttctgtt ggtcgattgt
    40141 ctcccattgg ttatttaatg acttttggag aagattttaa aaataagcca ttttacaaca
    40201 aagatcaacc tggtcgtgga tatgcttttc caggatttga tgtacctgta gagttttttg
    40261 cgccatttgt ttacaaagat tatcacttta tcaaagaatt tgagaaattt actgaaatga
    40321 ttgtctatga ttttacagta aaagaggata gtcaagatca aacgatggga gatgataatc
    40381 agttgttaca agaacaacta gagcgccaaa tgtatttagc aaaagatcca aaaggaacaa
    40441 gtttttctga tgcgcatatt ttgtaatcaa ctaaggaggt gaagttaaat ggcagagagt
    40501 tgttattata gagtagctac agcaatacga atgattaatc cttctctttc ttccagaacg
    40561 ttttatgatt ggttgaatcg tattgaacaa gtaactgatt accgcttttt gcgcaaagaa
    40621 cgtgttttta caggaaaagt tattaatcaa gtattgctaa cgaaaaaaga tatagaacga
    40681 ttgactcgat tgtatcatta tcgagtagat ttagaagagg atttaacttt gtcaatttat
    40741 cgcgtttttt ctcctgaaaa atatagtgaa ataacaaaat tagatcattt aattttgtag
    40801 ttattatcta tcgggagggc tctagacgta gtctggtagc cgcactctgc ctggcggtgc
    40861 gcattaaggc tgcgaagcat gggcctataa tgcgcactcg ctagcagcgt gtgcagctca
    40921 caaagaggtc gtgcgcttgc gcacggcctc tttgtgtaaa gccccccgat atataatatg
    40981 gggggcagaa ataacataac acatagaaaa aagttttata aacccttttg tatcaagtga
    41041 ttatctaagg tcagcagttg tactactttt cccaaaaaac gagggggact agttgatata
    41101 tcggtgtttc taaagggtta aaaaaatcga ttttgttatt ttataaaagg agcgagaaaa
    41161 tgacaccgat tgagctaaaa agctacagaa agaagttaag tgcaattcat aatataagtg
    41221 gagataaaaa gccgctatca caagcaagat ttgcgcaatt gatgggctgc tcaaaagatt
    41281 acattagaaa gatggaacaa gggaaaagaa aaatcccaga ggattttgaa caacgaatca
    41341 aggaacgctt tcgattgtat atgaaaagcg gaatagatct tcgagcaatg attgattatt
    41401 tgcgtctttc cttttttggg acagactaca aaacagtgat tcaacgtgta ttacgaatgg
    41461 atctgacaga atttgaaaca cgtccaacgg gcttgtatct ttttgactgt ctttctgttc
    41521 gtgggaatat ctgggtgttc tggcattcaa aacgaactgt aacagaaaat gtacttgtgc
    41581 aattttcagg acaaggttgc cgggaatatg aggaagtcat gcgtgaacaa caaacagatt
    41641 ggcgaacatg gctatattcc atttggaaaa atcaaggaaa gttggaagct ggttatacac
    41701 gtgtgcaatg tagccgattc gatattgcgt tagatgaact ttggggattg gaaacggaag
    41761 aacatttcga tctctttgaa ttactagaaa aacaaaaagc aggtttatta gaaatggact
    41821 ttaagagatt ccagaatatt ggcggttgtt ttttagatga tggctatttt cgaagtgaag
    41881 gattatcttt gtattttgga tcgcgaaaga gtcctctctt ttttaatttt tatgagaaac
    41941 gttttgaaat tgcgaaccga gaaaaaattt cagaattaga agcattaacg aaatatggta
    42001 tttataatcg atatgagtta cggttagcca atgagaaagc aacacaagca gtagaagcat
    42061 ttattttagg tgattcaccg aaacgtttag gagagattgg cgttggttta attaatgcaa
    42121 aacttattgt ttgggataga ctggaaagtg atgcacggat tgttgataaa cgatgggcaa
    42181 aattatttcg ttcgatggaa gaattggatt ttacagtaaa gccagaaaaa ttctcattag
    42241 atcgttcaga acgctggttt ttgaaacaag tagctccgac attaaagttg ttacaagaag
    42301 cagatcgtat tttaggaaga gactttgtac aagaagggat tgataatgcc gaactaaatg
    42361 acgagcaaca aaactatcta gaatggatgg gaacgtcagg agggaaaatt gcatgaatgc
    42421 atatacgaca acagaatggc tgcgcctgtt ggatttaaag gctgcaatcg aagcattaaa
    42481 tgaaaagatg gttgatttgt cttattttcg ttttcgagtt ccttatattg aacaagcggt
    42541 aaaagctgga cgttatcagg aaaaagagaa ctggcaagaa attgcacgct tattagaagt
    42601 tcgtaaggga tacgaacaag aactcgaaga gttggaattt agccgaagga aaggaaggct
    42661 agaattcatt cgcttttatc gattttcatt gccaatacct gcgattttag cggttaaaaa
    42721 aggctgcgat aaaatgaaga tatatgaaaa ctgtgtggca gcacttagca gcgaaaaacc
    42781 tcttatggaa gaaatttcat tggtgactgt cacttgggaa atgagtcgcc agccaacaga
    42841 agaacagaca tatctttctt tagaagagat tgagatagaa ttagaggaaa ttggtcgcta
    42901 tgccacttgt tctacctatt gtggatcggt cattagtatt gctggtgtga ttgtctaaat
    42961 gaaaaaaatt atgatttatg ttggtgcgta ttggtcacgt gatccaacag tattagaaaa
    43021 tccagaggcg gtacactatt gcttgcgcca attattctat ctgtataaag aacgattaga
    43081 atcgttgatt cgtcagttac catatactga cagacgttta gatgaacttc ttctaaggta
    43141 tccagcaatg tataaacgac gaaaaaatag attgttacca gaagagtatc ccattgaaaa
    43201 acatgaactt gaagggcgat ttgtggctta tttctatgat gatgttcgta tgcgcttagt
    43261 agaacaacga atggaaatag aaaatgatcg ctactatttt atcaattatt gtaagaagcg
    43321 taagtatcaa gtaactgatg atttttatga ttgtatttta caagatgggg aagtgattct
    43381 atccaaaatt gctccaagtt ttcgtgaact tgtgccgatt gattttttga aaaaatgtca
    43441 tttgcggatt cttccgtaaa aaagagctat aaaaggaaag aaaagtagaa gccaaatcgg
    43501 tttctgcttt tttatttttt ttaggaagga gaagatggta tgtcactgga atttttagga
    43561 gagccaattg aaaaggaaga gttcgtttcg cattatatgg atttgtttat tcaggtgata
    43621 aaaaactttg tgcgattgat gaattttatc cacgcgaaca agactatcta gccgctgaac
    43681 gaataaaaat cagtttatta tgggaaaaat tcacagatga aaataaccga ccgccagatt
    43741 atcgctattt atcagattgt ttaacgaata atgtgctttc taaagaatac ctatttcaca
    43801 aacgtgagaa aaatcaaatg tcggttgaac atttttgtga ttgttatatt gcattattgc
    43861 gtagtcaaca gttattaaca gtagaacgat atggtgcaga gtttggttat gtcttgcaag
    43921 ctgagcgtca tagagctaca agatgctatg aaaaagaaga tcaagtgatt gatggctatg
    43981 aagaattatg tattcgaaac aatcgctatt tgcaacaacg tttatatgaa caattgatga
    44041 tggatttttc aaatctctat agagggtatg aatgttcgat gattcaagaa aggtagaaga
    44101 aaagggggaa tttttatgtg gcgattatta tttttggtgg tatgtggtat ggcattgttc
    44161 tatgtgtatc gtgtctatct ctatccacga atggaacgat ggacaagttg gtatagagat
    44221 cctttaaaac aacgaaaagg attgaagcgg tatcgttatg aattacttgt tgaattttcg
    44281 gatgggcgac aagcacagta ttattttttt gcaaactatc caaaatacaa caccagtgaa
    44341 ctattgattc aattattcaa aaataatcca tatgttgggt tagaagaaaa cggacgggag
    44401 ttcttttatc gttcagataa tattagtgcg gcgtctatga acaaaacacg aattgcataa
    44461 aaaattggag gaataatcat gaaaaaaaca acattagcag tcgtaacaac actaggaatt
    44521 tcattatttt cgtttcaatt agggagttca ttggtttatg cagatgaaac tgtagaaaca
    44581 cctaagtcag aaattgtaga aacattgcca ggggaaggat taccagatgt tgagacagag
    44641 ctaaatggag gaacaacgga aaaaccagca gaacctgaac aattgcagtc agagaaacct
    44701 gaaaaagagc cagtgacacc agaaccagag tttcctgaat caactttacc gactgattcg
    44761 atcgaagaag aaggtgtgca tagttcaaca acaacggatt caagtatagt cacaactccg
    44821 gaacaatcag gaacaaccag ttcttctgaa aagaatactg attcgtcaag tacggaacag
    44881 ccaacgatgt cgtctaccag tgaagaacaa aaagaagaac cgaaacaacc tgttgaaaaa
    44941 tcagaagcaa ttccaaaaga aaataaacct attgtttcag caaaagaagt cacagtatcg
    45001 gtaaccccaa gcggtgaaat tacgacgaat acttctcaag gaatgagtgt accgattgtc
    45061 actagtaatg tagaggaatt gacccatatt ccaacaccaa ccaccccatt aaaagtagca
    45121 ggtgatcaaa caattgttgg tgtaaaagat ggtattccat taatccaaga tagtgaaggg
    45181 aatttggtaa aggatttgtc gattcctgta aagaaacttc caagtggcaa tattgaaatc
    45241 aaaacagcag atggaaaaac aaaagtctta ccaaagactg gtgaagaaat acatgttgca
    45301 ctttctgttc taggaggagt gttgacaagt attgctggat ttgttggata taaaagaaag
    45361 agaaaaggaa tagaaaatga gggtaattga atggactatt tttcagaaaa acaagcttat
    45421 cgttgtcaat cgtacgtact agtaaaaaca tactagaagg agtagcttat gaaaaaacga
    45481 caactacggc ggaaagaaaa agtttataca gagaaagtac cgaagcaaaa gaaaatccat
    45541 tcaaaaaaag cagtgaaatg gatttggtgt ttggttttat ttctttgttt ttcagggggc
    45601 ttggcatttt ttcaatccat gaaaacgaag gttcaagtgt cgtttttgaa agatgaaatc
    45661 caacaattac aaaagaaaga agtacaaagt cggtcagaat taacgtacac acctgaaatt
    45721 gaatccttta tgaatcgttt tgttgctttg tatatgaata cattcgataa tcatgaacaa
    45781 gaacaacaac gtcaagaaac attaaaaaaa gagttttatg cacaaaactt tattcaagaa
    45841 ccaggaagtg tttcaggtgt tcgaaaatta actgctgctt cgtttattgg attaaaagaa
    45901 attgaaacca ttccaactgc tagttataaa gtaacttatg atattcgctc gatgaatcca
    45961 gagagtaaga aagaaacaag tgtgacaatc acacaagtac tcaatcttcc cttttctatt
    46021 tcaaacaagg gctgtcgtat tattgcttat ccttatttta cggcactacc aaagaataca
    46081 gaaaaacaac gaatgattac ttatgatgac acattatacg aaacggttga tgcgtcggaa
    46141 gcgaaagatg ttgaaggatt tgtggaagat ttcttagata agtatgcgaa aaacaatgca
    46201 aaagatatgt catacatgat gaaagaacct gaaggactca atcaaacagc taaagttgat
    46261 caagtagaga tgaaagtatt gcgttataaa gaaacgaaaa aaccttttat tgtaaaagca
    46321 acggtcgtct ttcgagatgg tgatacgtca cttttacaaa aagaacaaat gacgattgtc
    46381 attacgaaaa aaacaaatca gtattatgtt gaaaaattaa cacataattg gttggatagg
    46441 aaaggagaag aacggaaatg acactgaagg gaattgtaga ttttatatta acgcaaggtg
    46501 gctatttttt aataatggtg gttgtttatc tgggattgcg aaattttatg aaatcaaaag
    46561 taggaaatgc attaatatct atcgcagcag gtgccgcagt ttatttcttc ttaaatgatc
    46621 caacacgtgt atttgatgcg tttggtaaaa tcattgaaaa attatttatg gattaggagg
    46681 atagtgaagt ggattgggaa ccttatgatt attcgattgt ttatgcggaa ccttatcgcg
    46741 tacaacagat tacaagcaat tggcgtcttc catttccagt tgcattagtc aatgcagcaa
    46801 cttttttgtt ggtcgtaatc tttgaatatt tatttttacg ttcatttatt ggttgggtca
    46861 tttctttttt tccaccagca ggagttgttt tttatatagg actcccctat ttatgtgtgt
    46921 tagcactggc aaaactaaaa cctgatggaa aaaaagtgca tttttattta tgggattggt
    46981 tttgttatat gattcgtatg atgaatggtg attgtgtgta ttataactgg gacaaggtat
    47041 caaacaatgc attggaagaa ttactttttg tgaataagta tgtttattta gaggattaga
    47101 aaaagggaca atcaactggt tgttcctttt ttattgggag gtgaaattca ttggtgcttg
    47161 taaaagaacc agtaaaagca ttcaagaaaa attgtttgtt aaatggagag gattgtatct
    47221 gggcctacta tcatgttttt ccacgaacaa tttcaaaaaa cacaccaaaa aagacacagg
    47281 aaataaaaga aatgatggct cgtttactgt tgcttttagc agattatgaa gatgtggagt
    47341 tggtgatgta tccacgaaca atgaatttac ggacgaagtt tgacacatta ggaaagggat
    47401 tttctcccga tagtcaagca attggtgcgt attatgcaaa agaaacagtg acattgttgg
    47461 aacgagaagg tcaagtctat cgtccttcat ttattttagg aatcaaagtc aaagaaaaag
    47521 caacggtgga gagttttcat gatgtaataa atctaatctg gttagattta gcaggaagtt
    47581 tagcacgctt atttggatta gaagtagatg atttggaaac tttgttttct gaaagtgatc
    47641 cttatgtaaa agaagtgaca caattacttt atagcttttc tattcgtccc ttagtaaaaa
    47701 aagagctgtt gtatctcaat cgtctgcaat atattcgagg aatggctcac aaagtagaag
    47761 aacaaaataa ttgggaagta ctgaaagaca atcaaggaac gtatgtacgt ataccgaagg
    47821 aacataaagg ccttcttcaa ttagaagatg agttagggag tagttattta gcattgttgc
    47881 ctattgggga aacacctgaa aatatgagaa attgtgatct atttcaattt gctcaggatc
    47941 tttctttccc agcagaattg cgaatgaaaa ttcactatcc acgcttgaaa ggacagggag
    48001 gtattggttt aaaactgaat tggctatcgg aacggttcaa aacagagcat aaggaattgg
    48061 ttcaaaatga tgaagaacct agtgaacgct tattaactgt tcgaggatta gtaaaacatt
    48121 taaagcaacg agtagataat gggcaacctg tcgtcgattg gatcgcatgt tttattgtat
    48181 acggtcaaac ggttgaagaa gttcgtcaaa atagtgcaac ggttattcaa caacttcata
    48241 cgattatcca tatccaccgt gcaaaaaatg atcaacgaga attgttctat aaatgtttat
    48301 tagggcaacc attgggacgg cagttacatt ggaagcaccg aacaactgta gaagcagttg
    48361 gcgaatggtt gttttcagca gtatcagaat taggtatgga atccggctgg tatattggtc
    48421 ggcaagatac attaggggat ggtggttctc gaggagaaaa aacaccatta gaagaattaa
    48481 tttatgcgtc aaatcgtttt gtctttttga atcttctagc cattgcggaa ggaatcaaag
    48541 gtgcgttgtt tgatgcgcct catgtagcag taactggaaa aaccggtaaa gggaagacct
    48601 ttttagttgg cttgctcttt ttgtatagtt cgtttatgaa tgtacaaagt ttatttgtgg
    48661 atcccaaagg agaaaagcgc aaatggttta cgaaactcat tcaagacccg tattatcaga
    48721 aacattaccc tttgtttgta gaacatttgc aaaaaatgag ttatgtcacg ttagatgcta
    48781 caaatccccg taattatggt gtattagatc caattgtttg tttatcccga gtagatgcga
    48841 aacaagtagc ccaggatatg attgatgagt taagtccgtt aggtaccaat caccttttaa
    48901 aaggggcggt attaaaggga atccaagaag ttttagttca aaaagaagcc ggacaacaag
    48961 ttggcttgat gcatgtggtc gactatttag aacaaatgga acaaaaagag gttcgagaat
    49021 atggtgagtt tttacgcttg acggtagaag attcgatttt acgtttagga ttttcttatg
    49081 gagaaaaccc gggattagac tttaatgcca aaacaacgat tttagagatc caagatttga
    49141 aattaccaaa tgacaatgtc cgaccagaac tatatacaga tgcggatcga aagtctttgt
    49201 gtttgatgat tagtttaggt cgtttttgtg aaatgtttgg aaaacgagat agtagtaaga
    49261 aaacagcgat ttattttaca gaagcatggg tatttaataa ttcgaacgca ggacgtagta
    49321 ttattgcagc aatggcacgt gtggggcgtt cccagatgaa tcaattagtg ttagatacac
    49381 aatttattgg tgacttaggt tcagataaag aaaaagggaa ttttggtgtc gtctttgctt
    49441 ttgatgaaga cagtgagcga gaaaagattt tgaatcattt acgtttggaa gtaaatgaag
    49501 cgaacttagc tgaaatgagc cacatgatta aaggacagtg ttggatgtta gatccctatg
    49561 gacgtgtagg aaaactcaat gtacacagtt tgtttgaaga aatgaccgct gcattacaaa
    49621 caacggaaaa aacaagtcac tctcaagtag aagaacagga tacgttaatc atgcaataga
    49681 aatgaggtga aacaatggct tatataaagc ataaggaaaa gaaatggtgc gttttgtttt
    49741 ttttaacgat tggattcgga ctgctttttg ttggtggatt ggtgtatgcg gcagaaaagc
    49801 aggtggatca atcgatctat gagattggga aatttcatat tgataccttt tcttcttatt
    49861 tatctgatag tgaattttca ttaggcaaaa tgttatttgg ggcaaatggc acagattttc
    49921 taaaaatgat gacgaatatg ttctttagtt taagtaaatt gatttggcaa ggccttgatt
    49981 ttgtcattga gaagctctat gaaggagctg caatgaacga gttaattcac cagtttttca
    50041 ctttttcgga tactatttat tcaaaaatat acgatcctat tggtgcgggg attgtcgtct
    50101 tgtatgtgtt atatctattt actcttcgta tatttagagg gaaacattac gcaaaaatgg
    50161 tgtttattcg atttctcttc gttgtcattt tttcaatggt ttggtttggc tatggtagta
    50221 acgtacctag taaaggagaa gcttttacca aagggttaaa tgatttgtcc gtggaggtag
    50281 aaggctttat tttttctgct acaaatagtc tagatgaatt ggcgacaaaa gatgtcaatg
    50341 gtgttttagt gccaacgaca caaaaggaag ccattcaaaa agtgcgccaa ctttattata
    50401 aagcggctgt tgttgatccg tatatattgt taaattatgg gacaacaaat ttagaagaat
    50461 tgtatcaagc agagattgaa ccacaagaat ttctagccaa ggaagcgtca agtgaagaat
    50521 tatctgatat tgagacaaaa gtgaccactg ctgcaggaga agatagaggg aaaaagaagg
    50581 cttatcgtgt ctatattcaa ccaactaaag ctgtttataa agcagtcgtt ggttctatgg
    50641 tgccattgct taatctaagt attggattac cacttgtagt gattggcatg attcgtttta
    50701 tctttcaaat gttggtgttg tgtttattac ttgtgattcc tttttcatta ctcgcttctt
    50761 ttgttccagt atttgattat atgctcatga ataccttaaa gtcctttttg ggcttgatgt
    50821 ttcaaaaatc tttttacagt gtgattattt tggtggcatt tttgatctat aacatagtag
    50881 acgatttaat tccaatgaat actgtttttg gttttttagg aaatatgttt gtcaaagcac
    50941 tcctatcaat tttactcatt gtaaaacgga aagatttatt aaagaaacta gggttaggag
    51001 ctgcagatca aactataaac aacacgaaaa acatagcaag tcattctttg tctcaaatga
    51061 aacaactgcc tggtcaacta tcaactgctg gacaaaattt ggcaatcaaa ggtgcaggat
    51121 tagcagggaa tgtgtatcga gcaacgaatc cactcgcttc tgcagcgata catggatttc
    51181 aaaagtttca acaaaaacgt acaccacaag aatcggtaaa agggaggcaa gtggttaatc
    51241 cctttgaact aggtgggcgt tcaaatcaag ccttgcctta tagtggtcgt catgttcgtt
    51301 atcatggggt agagcctgaa atgtcttcta ccaaatcgaa aacagagtgg aattctggtc
    51361 tttcaaatgg tttgattcca aattcgtcca tgaattcagt aagaccaaaa caacaggtac
    51421 aatcattggc acaattgagt aagcaacaag ggaataatca aagaaaacca ttgttaaata
    51481 aaggtatcgc ttttgttggt caatcgattc cgttgccttc tggaaaagta cccgtgccac
    51541 aattaacagc tgctggttct ctttcacaac agattcgcca agtgaaaaat caggcgaaaa
    51601 atgggagtta tccagcaatg cagcagttat tgaaccaata ctctgttcct aattcgtcgg
    51661 tagccactct atcaggaaat cgttctgtgc aacaaccggg gcaacgttcg tctgaaagta
    51721 aaccgtcgat ttccgcacca aaatcgttac aacctgcatt ttcttcttta gataagccaa
    51781 cagagcaatc ggaaaaaata gaaaaggatc ctaaaacact gccgattttg gacgcaaaac
    51841 caggaactga ttttacttta ccaaaaggac gaacagtcca aaaaccatta tcgaagaaag
    51901 gatgaggaag aaatgaagaa agggtgtcta ggttgtcttc ttttctttat tcttcccttt
    51961 tttctattgc cattcttttt ttcaggagaa ctttctgagc atggaggaga agctacaggt
    52021 ctagctgtcg cttttaatgg ggaatatacc gaaggattac cgattttttc agagattaaa
    52081 ggacgtggtc caatcacaga tgaacatgcg caagcagcag ttggtgcagc tgtaaagtat
    52141 cagttattac cgagtgtgat tctttctcaa ttaggctatg aatcagctta tggacagtca
    52201 caatctggaa gaaacgatac gaactttttt gggattacct ggtttcctgg ttgtccctac
    52261 ccacaaggtt ctgcacgtgg aattggtggt tctgaaggtg gtttctatat gaagtttccg
    52321 aacttaaaag cttgttatga ctattatggc tttatggtgg cgagccaaag caactttaat
    52381 caatgtgtgg gcaataaaga tcctggaagt tgtttattga ttctaggtcg tggaggctat
    52441 gcggcagcag gtattaccga agggagtccg tattatcgag gagcaatgag tatcataacg
    52501 tcctataact taacggaata tgatactttt gcgatcaagc attggaatag cctaccaaca
    52561 aaaaaaactt cgcttattgc aggaaaaagt aaaggcaatg cgaaggtttt ggatcggatc
    52621 attggacgat ccgtaaataa tggggaatgt tatggtggta cggcatggta tgtatctcaa
    52681 ttaggtggcc cacagttaat gggttctgga cacatgtttg cgatgttaat aggggaagat
    52741 tataactgga caacctatgg ttggcaagta atcatgaacc caaaaccaag tgatttacaa
    52801 gcaggagatg tggtgaattg gcaagcaggt ggtgcattgt ctcctggaat ctatggtcac
    52861 accggaatta taacagatgt ttcatatggc ggacaacatt tctctagtgt ggaacagaat
    52921 gccgagcaag gacggattat ggctcggtat caacgcacgt acaatcaaac aaaaattcgt
    52981 agtctggttc gtaaggtgaa ataagaaagg cagtgactag aaacgtgaaa aaagggataa
    53041 ttttagtatt tagttggtta tgtttgatag gtcttactgg ttgtcattgg ttttcttctg
    53101 aaaaacaaaa aacacaattg agaacgacag aagaaacaat cgaagcagta aaagaaacaa
    53161 gtggaactct tcaaagtact gaaagtaaca gtcaaattgg ttcattagaa gacgagacag
    53221 cgcaacaaat gcaaatagat gaaatgacaa aaaatttctt aaaagatttt gctagaaaat
    53281 ggagtaattt cacagatgtt tataaacgca atcaagctgt gagggactat atgacagatc
    53341 gttgtattaa agacaatagt attgatgtag acccgcatgt acaaatgaaa acaactggga
    53401 aactttacca ggtgacacaa gatatagaga atccggatca atatttgtta tttggagaag
    53461 aaacaatgaa taaggttact caatttgtat tacttcaatt gaaagtaggt caagaagctc
    53521 aaaagattga tgcgatcaag atttattatg tacggagtgc gtattaaaat aaaaaatgaa
    53581 cactcatatt tattatacac cgaagaaata gaaaaaaaca gactgttttt ttaagaaaaa
    53641 ctatgtcatt ctttacttga aaaaagttgt tataggacag aaaggataaa acaaaaacgg
    53701 aaaaaatatg tcttatgaat aactataaga actattctat tttttaggag atggatacga
    53761 gatgaaaaaa ctaattggct atagtgtgat gtgtattgga tttgttggtg tgggattgtt
    53821 tgtatcgact gaatctgctt ctgcggctga atggtataat cctatctggt ataatccgaa
    53881 cattgaatgt caaaaaggct atccgtatgg accgacaaat ctgaaaaatg gaaaatgtag
    53941 agtaaattgg aatggcgttg tagatagtgt aattactggt tcaatggact ctgctctaca
    54001 tggctttacg ggtcactaga aggaggaaga aaatgtctga agagaaaaaa gaaatcgttg
    54061 aatcattagt agctttaaaa gattctcttt ctaagattga atatgttgat aggcaagaaa
    54121 ttcaaaaact aattgatgat actattattg aaatccaaga tgcgcgttgt gaaggtataa
    54181 aaatttctgt agctttatct aaagtaatag aaaaaatgaa ccgttcatta gcgtttaatg
    54241 gattgaagct agatagacaa actagtctga tatgggatca tttaaaagac ttgtatgaca
    54301 agtctaaaag aagtgaacgg actgctgtat caatcttaaa aggcctttgg ggaatgaatt
    54361 cataaacaag atatttaaaa agtaacttat tttttatgga ggagaaatta tgaaaagaat
    54421 aattaaaata tttggtgttt taggtgtttt cttattaggt ttaagtactt ttgttagtac
    54481 tggaacaagt gtgtatgcca cagaaaaagt gaatacagtt gatttagaaa taaagaataa
    54541 attagtagat gaattaactt tttattttga tacagttggt tttttagacg aacatggtgt
    54601 ataccatata aaggattttg gtgctattcg tgaacgaatc agtcaagata acgaacaagg
    54661 aagagcagca agagtctttt atgaacatat tattgttcct agaatggaga aagttgattg
    54721 gaaacgatat gctggatgtg tagccattaa ttctcttcct ggaggaggag tggcttgggc
    54781 tataggtacg cagggagatt ttattgaagc attaaaagct tttaattttg aaaaagctgc
    54841 ggatttatta ttaaaaattg cagaaaaaca attaggaaaa gctgcagcta aagagttagc
    54901 taaggtaaat attgtagcag gaattgcagc agctgctgct agctgtgcat tagagtaaaa
    54961 gtttatgaaa agtttagaac aagagcatat ggcttttaaa caagcaatgt ttaaagaaaa
    55021 tatctattta aaccataact atattcgtgt ttctaaggca tgttcaccag tattgaatat
    55081 gcttggaggt ggaaatggat tatatcattt actgtttgtc gatgtttgtt ggttaatttt
    55141 tttaccagat gaactagtta tagtcaatga aaaaataaca
//
LOCUS       DAZBSA010000005        52351 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000005 DAZBSA010000000
VERSION     DAZBSA010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.005"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            545..883
                     /locus_tag="ACHGMT_000271"
     CDS             545..883
                     /locus_tag="ACHGMT_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834049.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            977..1207
                     /locus_tag="ACHGMT_000272"
     CDS             977..1207
                     /locus_tag="ACHGMT_000272"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFN6834050.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            1607..1978
                     /locus_tag="ACHGMT_000273"
     CDS             1607..1978
                     /locus_tag="ACHGMT_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFN6834051.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(2252..2944)
                     /locus_tag="ACHGMT_000274"
     CDS             complement(2252..2944)
                     /locus_tag="ACHGMT_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN6834052.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(2947..3732)
                     /locus_tag="ACHGMT_000275"
     CDS             complement(2947..3732)
                     /locus_tag="ACHGMT_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFN6834053.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            3969..5369
                     /gene="lacG"
                     /locus_tag="ACHGMT_000276"
     CDS             3969..5369
                     /gene="lacG"
                     /locus_tag="ACHGMT_000276"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFN6834054.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            5394..5711
                     /locus_tag="ACHGMT_000277"
     CDS             5394..5711
                     /locus_tag="ACHGMT_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFN6834055.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            5724..7397
                     /locus_tag="ACHGMT_000278"
     CDS             5724..7397
                     /locus_tag="ACHGMT_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFN6834056.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            7411..9042
                     /locus_tag="ACHGMT_000279"
     CDS             7411..9042
                     /locus_tag="ACHGMT_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFN6834057.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(9533..11098)
                     /gene="guaA"
                     /locus_tag="ACHGMT_000280"
     CDS             complement(9533..11098)
                     /gene="guaA"
                     /locus_tag="ACHGMT_000280"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFN6834058.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(11157..12080)
                     /gene="coaA"
                     /locus_tag="ACHGMT_000281"
     CDS             complement(11157..12080)
                     /gene="coaA"
                     /locus_tag="ACHGMT_000281"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFN6834059.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            12276..12881
                     /locus_tag="ACHGMT_000282"
     CDS             12276..12881
                     /locus_tag="ACHGMT_000282"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN6834060.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            13189..14205
                     /locus_tag="ACHGMT_000283"
     CDS             13189..14205
                     /locus_tag="ACHGMT_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN6834061.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            14346..15647
                     /locus_tag="ACHGMT_000284"
     CDS             14346..15647
                     /locus_tag="ACHGMT_000284"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFN6834062.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            15693..16355
                     /gene="deoC"
                     /locus_tag="ACHGMT_000285"
     CDS             15693..16355
                     /gene="deoC"
                     /locus_tag="ACHGMT_000285"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFN6834063.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            16376..16774
                     /locus_tag="ACHGMT_000286"
     CDS             16376..16774
                     /locus_tag="ACHGMT_000286"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFN6834064.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            16840..17922
                     /locus_tag="ACHGMT_000287"
     CDS             16840..17922
                     /locus_tag="ACHGMT_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN6834065.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            18134..19696
                     /locus_tag="ACHGMT_000288"
     CDS             18134..19696
                     /locus_tag="ACHGMT_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6834066.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            19689..20822
                     /locus_tag="ACHGMT_000289"
     CDS             19689..20822
                     /locus_tag="ACHGMT_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6834067.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            20822..21778
                     /locus_tag="ACHGMT_000290"
     CDS             20822..21778
                     /locus_tag="ACHGMT_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6834068.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            21877..23043
                     /gene="deoB"
                     /locus_tag="ACHGMT_000291"
     CDS             21877..23043
                     /gene="deoB"
                     /locus_tag="ACHGMT_000291"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFN6834069.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            23067..23885
                     /locus_tag="ACHGMT_000292"
     CDS             23067..23885
                     /locus_tag="ACHGMT_000292"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFN6834070.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            23902..24609
                     /gene="deoD"
                     /locus_tag="ACHGMT_000293"
     CDS             23902..24609
                     /gene="deoD"
                     /locus_tag="ACHGMT_000293"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFN6834071.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(24907..25593)
                     /gene="gpmA"
                     /locus_tag="ACHGMT_000294"
     CDS             complement(24907..25593)
                     /gene="gpmA"
                     /locus_tag="ACHGMT_000294"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFN6834072.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(25765..26346)
                     /locus_tag="ACHGMT_000295"
     CDS             complement(25765..26346)
                     /locus_tag="ACHGMT_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN6834073.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(26479..27162)
                     /gene="rpiA"
                     /locus_tag="ACHGMT_000296"
     CDS             complement(26479..27162)
                     /gene="rpiA"
                     /locus_tag="ACHGMT_000296"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFN6834074.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            27481..27894
                     /gene="rpsL"
                     /locus_tag="ACHGMT_000297"
     CDS             27481..27894
                     /gene="rpsL"
                     /locus_tag="ACHGMT_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFN6834075.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            27978..28448
                     /gene="rpsG"
                     /locus_tag="ACHGMT_000298"
     CDS             27978..28448
                     /gene="rpsG"
                     /locus_tag="ACHGMT_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFN6834076.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            28542..30629
                     /gene="fusA"
                     /locus_tag="ACHGMT_000299"
     CDS             28542..30629
                     /gene="fusA"
                     /locus_tag="ACHGMT_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFN6834077.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            30801..31988
                     /gene="tuf"
                     /locus_tag="ACHGMT_000300"
     CDS             30801..31988
                     /gene="tuf"
                     /locus_tag="ACHGMT_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFN6834078.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            32395..32703
                     /gene="rpsJ"
                     /locus_tag="ACHGMT_000301"
     CDS             32395..32703
                     /gene="rpsJ"
                     /locus_tag="ACHGMT_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFN6834079.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            32734..33363
                     /gene="rplC"
                     /locus_tag="ACHGMT_000302"
     CDS             32734..33363
                     /gene="rplC"
                     /locus_tag="ACHGMT_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFN6834080.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            33391..34014
                     /gene="rplD"
                     /locus_tag="ACHGMT_000303"
     CDS             33391..34014
                     /gene="rplD"
                     /locus_tag="ACHGMT_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFN6834081.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            34014..34304
                     /gene="rplW"
                     /locus_tag="ACHGMT_000304"
     CDS             34014..34304
                     /gene="rplW"
                     /locus_tag="ACHGMT_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFN6834082.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            34343..35176
                     /gene="rplB"
                     /locus_tag="ACHGMT_000305"
     CDS             34343..35176
                     /gene="rplB"
                     /locus_tag="ACHGMT_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFN6834083.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            35219..35497
                     /gene="rpsS"
                     /locus_tag="ACHGMT_000306"
     CDS             35219..35497
                     /gene="rpsS"
                     /locus_tag="ACHGMT_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFN6834084.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            35520..35867
                     /gene="rplV"
                     /locus_tag="ACHGMT_000307"
     CDS             35520..35867
                     /gene="rplV"
                     /locus_tag="ACHGMT_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFN6834085.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            35881..36537
                     /gene="rpsC"
                     /locus_tag="ACHGMT_000308"
     CDS             35881..36537
                     /gene="rpsC"
                     /locus_tag="ACHGMT_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFN6834086.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            36540..36974
                     /gene="rplP"
                     /locus_tag="ACHGMT_000309"
     CDS             36540..36974
                     /gene="rplP"
                     /locus_tag="ACHGMT_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFN6834087.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            36964..37152
                     /gene="rpmC"
                     /locus_tag="ACHGMT_000310"
     CDS             36964..37152
                     /gene="rpmC"
                     /locus_tag="ACHGMT_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFN6834088.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            37177..37443
                     /gene="rpsQ"
                     /locus_tag="ACHGMT_000311"
     CDS             37177..37443
                     /gene="rpsQ"
                     /locus_tag="ACHGMT_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFN6834089.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            37501..37869
                     /gene="rplN"
                     /locus_tag="ACHGMT_000312"
     CDS             37501..37869
                     /gene="rplN"
                     /locus_tag="ACHGMT_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFN6834090.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            37906..38214
                     /gene="rplX"
                     /locus_tag="ACHGMT_000313"
     CDS             37906..38214
                     /gene="rplX"
                     /locus_tag="ACHGMT_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFN6834091.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            38242..38781
                     /gene="rplE"
                     /locus_tag="ACHGMT_000314"
     CDS             38242..38781
                     /gene="rplE"
                     /locus_tag="ACHGMT_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFN6834092.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            38800..38985
                     /locus_tag="ACHGMT_000315"
     CDS             38800..38985
                     /locus_tag="ACHGMT_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFN6834093.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            39021..39419
                     /gene="rpsH"
                     /locus_tag="ACHGMT_000316"
     CDS             39021..39419
                     /gene="rpsH"
                     /locus_tag="ACHGMT_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFN6834094.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            39451..39987
                     /gene="rplF"
                     /locus_tag="ACHGMT_000317"
     CDS             39451..39987
                     /gene="rplF"
                     /locus_tag="ACHGMT_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFN6834095.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            40148..40504
                     /gene="rplR"
                     /locus_tag="ACHGMT_000318"
     CDS             40148..40504
                     /gene="rplR"
                     /locus_tag="ACHGMT_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFN6834096.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            40525..41025
                     /gene="rpsE"
                     /locus_tag="ACHGMT_000319"
     CDS             40525..41025
                     /gene="rpsE"
                     /locus_tag="ACHGMT_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFN6834097.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            41040..41219
                     /gene="rpmD"
                     /locus_tag="ACHGMT_000320"
     CDS             41040..41219
                     /gene="rpmD"
                     /locus_tag="ACHGMT_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFN6834098.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            41263..41703
                     /gene="rplO"
                     /locus_tag="ACHGMT_000321"
     CDS             41263..41703
                     /gene="rplO"
                     /locus_tag="ACHGMT_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFN6834099.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            41703..42998
                     /gene="secY"
                     /locus_tag="ACHGMT_000322"
     CDS             41703..42998
                     /gene="secY"
                     /locus_tag="ACHGMT_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFN6834100.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            43060..43707
                     /locus_tag="ACHGMT_000323"
     CDS             43060..43707
                     /locus_tag="ACHGMT_000323"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFN6834101.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            43898..44116
                     /gene="infA"
                     /locus_tag="ACHGMT_000324"
     CDS             43898..44116
                     /gene="infA"
                     /locus_tag="ACHGMT_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFN6834102.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            44149..44265
                     /gene="rpmJ"
                     /locus_tag="ACHGMT_000325"
     CDS             44149..44265
                     /gene="rpmJ"
                     /locus_tag="ACHGMT_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFN6834103.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            44283..44648
                     /gene="rpsM"
                     /locus_tag="ACHGMT_000326"
     CDS             44283..44648
                     /gene="rpsM"
                     /locus_tag="ACHGMT_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFN6834104.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            44676..45065
                     /gene="rpsK"
                     /locus_tag="ACHGMT_000327"
     CDS             44676..45065
                     /gene="rpsK"
                     /locus_tag="ACHGMT_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFN6834105.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            45145..46083
                     /locus_tag="ACHGMT_000328"
     CDS             45145..46083
                     /locus_tag="ACHGMT_000328"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFN6834106.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKNLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            46111..46491
                     /gene="rplQ"
                     /locus_tag="ACHGMT_000329"
     CDS             46111..46491
                     /gene="rplQ"
                     /locus_tag="ACHGMT_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFN6834107.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            46763..47602
                     /locus_tag="ACHGMT_000330"
     CDS             46763..47602
                     /locus_tag="ACHGMT_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFN6834108.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            47578..48447
                     /locus_tag="ACHGMT_000331"
     CDS             47578..48447
                     /locus_tag="ACHGMT_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFN6834109.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            48437..49234
                     /locus_tag="ACHGMT_000332"
     CDS             48437..49234
                     /locus_tag="ACHGMT_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFN6834110.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            49437..50183
                     /gene="truA"
                     /locus_tag="ACHGMT_000333"
     CDS             49437..50183
                     /gene="truA"
                     /locus_tag="ACHGMT_000333"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFN6834111.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            50847..51041
                     /locus_tag="ACHGMT_000334"
                     /pseudo
     CDS             50847..51041
                     /locus_tag="ACHGMT_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(51229..52005)
                     /locus_tag="ACHGMT_000335"
     CDS             complement(51229..52005)
                     /locus_tag="ACHGMT_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFN6834112.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            52128..>52351
                     /locus_tag="ACHGMT_000336"
     CDS             52128..>52351
                     /locus_tag="ACHGMT_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFN6834113.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTVKLNMEKLGFSIHAFLIIITQN"
BASE COUNT        16675 a         9435 c        11316 g        14925 t
ORIGIN      
        1 tgtagaagcg agcaatacgg ttgcaaataa ccattatcgt ttaataagca gttacgaaag
       61 ctcccaatat ctctaacgtt cagctcactc catttctttt cttcaaaaaa atcagtgatt
      121 cgtatctctt tctatttgtc gaaaaagtta actttaaaaa gtaacgccga aaaaatatgc
      181 aaaaaaataa aaaagcagtt actattgcta gaataacatc aaggaataat aagttttggt
      241 tttcagataa tagtcgataa gttgtgtatt ggaattttag atacagtaat aaagtatttt
      301 tcatgaaatt ttgtgagaat cactattaca agtatcttta ataaaattaa tggttaagga
      361 ggaacttaaa gtagaatgta aatgaatagt tgtagtcagt cgattgtgat actagactca
      421 aagttgcagg aataagtttt tcaatttgtg attggaagat aaaaaatgaa aaagaagggc
      481 ttttttttat actttgttga ttgtagtgtt tattttccct ctgtttagcc aagtaacatt
      541 ttctatgact caaccagttt caagtgagga acatgataac ctttttcgtg ctacagaaga
      601 gtttaagaaa tatcaggaaa atattgttcc ggagactcca ttagctagtg agccaattac
      661 tgatattgat ggaaaccaaa taggatggta tatacagtac gaagttatgg tggtaaataa
      721 tgactcaata gtaaaaaata cagtaggctt agagctaaca ccgattgaaa aggtgagttc
      781 attgttaacg tatatttata ctgatgaaag tgacgagact gagatcattt taacggatta
      841 ctcaaaaatt gctactgata aaaagtatat attgtggatg tagacactag tgaaatactt
      901 tatgatatag aaattgatga aaatttacta gatgtagcac aagaaatcga tcatcaaaag
      961 caggaagcta ttcgatatgc tggtagaaaa gggaaaagaa aatatttatt acgtgaatgt
     1021 agcaaaagtt agagaagatg aaaatgagtg gaaagaattc aagagtcgct actcaattaa
     1081 ctcgactccg acatttacag tttacagaga aggatcgata gaaaaaactg ttttttggac
     1141 taaagaaagt ggaatgtctt tagctgaagt agaagagttt ttggattatg ttagtatgca
     1201 acagtaaggg agttcttatt atgaaaaatt ttgacacata ttatgttatt gttttttcga
     1261 tatctttgct gttaatatta ctaactttaa tgcagtataa agagtatcca gagtatttta
     1321 ttttaacttt tgtatattta gtaagtagct acacacttct atataaattc agaagttctt
     1381 ctagagtttc taaagtttgt ttcttaatta gcttattgat tctataatat ttttgattta
     1441 tataggaaat taaaaatatt atagaatcaa cactcagaga acgggttggt ttttaataga
     1501 tttaataagt actgataatg aaatttctcc tggttgggaa aacgaagttg gttaccctgt
     1561 agaagaattt tttcaagatt actttgcgga gtatgccgat aggataatga gagattacgt
     1621 aacagataaa actcaagaag tcatcaatca atcccagtca ttaaatgaat tatctgataa
     1681 ggcgaaagaa gaagcagttg ccattatttc ttcagggatt agtgactata ttgatatgag
     1741 tggtgaaatc aattatggcg ataaagacaa aattgtacgc atgataaaag cttttatcaa
     1801 aacttttgat gtgaaggcag atcttaagtt taatgatact tacctagtag gaaaattaat
     1861 taatatcctt gggacagaga aagatacgga aacgtttatt tcctctttgt ctatgttgtt
     1921 tacagataag catttttcaa ctcagcttgg tggagaggaa ctagtggaaa tcttttaacc
     1981 attacgccca gcattaataa aagtatatca aaaaagtact cctgatgatt tttaagattg
     2041 gtttgagaaa taatgtgaaa acactatttt taatgttaat tatagataga gatgaacgcg
     2101 tcatatggtt aaaagaatga aaaagtcaaa gatacactgc tatatttcac taaggatata
     2161 gcagtgtatc tttcatgtcg tttcactggc agttttttat aaaaggaggt gtgcatttca
     2221 gttgcttttc tttggtagtt agttgaatcg tttaagaatt aacggatgtg gcagacaggt
     2281 gctctgtcca aaaaccagta aaatctttga tggttccatc tttatttagc tgtgcctgtt
     2341 caaatactgt tttgttttta ccgaatggat caattgcaat aattgttccg atccccgcac
     2401 gtttggcagc aagtaatcct gagtaagcat cttcgataac aagacaatca gccggagcta
     2461 agctcagttt gtctgccgct ttaagaaaaa tatccggttg cggtttccca gggaaagagc
     2521 cgtcgtcata gacaatcttg tcccagtcaa accagcgggc taaatcgaaa atatcaaaat
     2581 aaaaagcaac attctctttg acagtagccg tggcaatcgt taatggaatc tgttggtcag
     2641 tcagctggtc aagagtcgct tcaagccctt ttgtcaactg aagctcgttt tcattttgca
     2701 agcaaagcat acgataatag ctttcttttt cttctgacaa tacggcgatc tcagaggcgg
     2761 tcaaatgctc tgaaatgaaa tgtttcaaaa tctccgtatt cgtccgacca tgaatatttt
     2821 ttaaaatatc ctcgtctgat aaaccatctt ttgtatgatc atgtatcata tgcagccaag
     2881 cagcttcatg cagatgagag tccaaaaaca ttgttccgtt gaaatcaaat ataatccctt
     2941 tcatagttac gctcctatag tggtattttc ggttgaatca gcaatttgca gatcgacata
     3001 ttgtgcatag tgctcttgaa cctcccgcga gatctggcga tccgtaatga gcagatcgaa
     3061 atcatgcaaa ttataaaaca cataaaaatc atctcgattg aattttttat tatcaacgag
     3121 tagatatttc ttacgggaat tatttagagc gatctgctgt gcttctcctt cttcggtact
     3181 gtaagtagag atcgcttcgt tgtgaacgcc atttgaactg ataaatgctt ttgtaaactt
     3241 tagtcttctt aaattgtcgt tggtgatcgg tccgacaaat gcgccggtat ttttccggaa
     3301 atcaccgcca atcagtagaa tttctggtcc atctgcttga gcaagaatat caaaaactgg
     3361 ataattattt gtaatgatcc gaattttctt gttcagtaaa tgcgtcgcca atagttcgat
     3421 tgttgtacct ggtcctaaga aaatcgtttc tccctcgtga atcaaggagg ctgctgtggc
     3481 agcaatttct tttttttctt gaatctgcag agtggctttt tcagtgtgag aaagttcttg
     3541 atctaaagtg taggaaagac tttgagcccc tccatggaca cgcagcagct tgcccgattt
     3601 ttctaattca tctaaatctc tgcgaacggt catatcagaa acatctaatt gattgatgat
     3661 ttcattaacg gtaaggattc cttttttatt gaccatttct acgatagtaa gcaatctttc
     3721 ttttttcaac attgtcttca cctctaattt aaggataatc tatcatataa acggacaaca
     3781 gtcaatctaa ttcgaacaaa agtaaacatt tagggtggtt tttatgtttg ttacaataga
     3841 ttttattgct gttttctggt ttttatgatt atttgtgttt gtttttgttg acaaatgttt
     3901 tggatgcgtt tacaatgcaa tcagttagaa agagcgaaaa ctgattatag atttgaggag
     3961 agatgtacat gcttaaatta ccagaagatt ttatttttgg cggagccaca gcagcctatc
     4021 aggttgaagg agccactaag gaaggtggca aaggcgctgt tgcttgggat gactttttag
     4081 aggaacaagg gcgattcagt cccgatccgg caagtgattt ttatcatcaa tatgcaaagg
     4141 acattgaatt gtgtgaacgt tttggtgtaa atggtctaag attatccatt gcttggagcc
     4201 gcatttttcc tgatggagct gggaaaccaa atccagaagg aatcgctttt tatcatcgtg
     4261 tgttcgaaga atgtaagaaa agaaacgtaa cgccgtttgt gacgttgcat cattttgata
     4321 cccctaaacg tttgtttgac catggagatt ttttgaatcg agaaacaata gaagcatttg
     4381 tttcctatgc cattttttgt tttcatgaat tcaaagaagt caaggtgtgg agtaccttta
     4441 atgaaatcta tccagttgca accaatcaat atttgttagg tgttttccca ccaggaatca
     4501 aatatgattt tacaaagatc gtcgcctgtc ttcataatat gatggttgct catgcccgtg
     4561 tagtgaacta tttcaaagaa aacgaacttc cgggtgaaat tggtgtagtt cactcattag
     4621 agacgaaata tgcggcaaca gacgcgccgg aagataagca tgctgccttt ttagacgatg
     4681 cattgtcgat ccgtttctta ttagatgcta cctatttagg ctattattca actgaaacct
     4741 tgactgctct tgatgagata tgtgaagcaa atcaggccag ctatcatttt cctgaagaag
     4801 atttcgttga attgaaaaaa gcatccacgc gtaatgacta cttagggatc aatcactacc
     4861 aatgccattt tgtcaaagct tacgatggag aaaatgcaat ccatcataac ggtacgggtg
     4921 aaaaagggac ctctgtttat aaagtgaagg gaatcggaga acgtatttat aaagaaggaa
     4981 ttcctcgaac tgattgggac tggctgattt atccagaagg tctatatgac ttgttgctgc
     5041 gaattaaaag cgattaccca cattacaaca aaatctatat cactgagaac gggatggggt
     5101 ataaagatca gtttgaagat ggcattatta tggatcagcc gagaatcgac tatttgagag
     5161 tctatttgga atccttaagc aaggcgataa cagccggggt aaatgtgaag gggtattttt
     5221 tgtggtcgct tatggatctt ttctcatgga caaatgggta taacaaaagg tatggcttgt
     5281 tttatgttga ttttgaaacc cagaagcgtt acccaaaaga atcagcttac tggtacaagt
     5341 tggtcagtga aaccaaaacg attatttaat aagtgataat ggaggaagaa aagatgaata
     5401 aagaggaatt acaaatgctt ggttttgaga tcgttgcata ttcaggcgac gctcgcagta
     5461 cattattgaa attattgaaa gaagtaagac aaggaaactt tgaacatgtt gattcggcgt
     5521 tgaaagaagc cgacgaaaat ttaacccttg cacataattc gcaaacaaaa attttagcgg
     5581 aagaagcatc tgggaaagag atggagatgg gattcatttt tatccatggg caggatcatt
     5641 taatgaccac tttattgctg agagatctga ttcaagactt tattgtactg tacagacaga
     5701 atcaaggata ggagaagaac gctatggatg gaatcattaa acaaatcgaa aaagggaaac
     5761 cattttttga aaaaatctca cgtaatagtt atttaggagc cgttcgtgac ggatttttaa
     5821 cagcgatgcc agctatttta ttttcaagca ttttcatttt gattgctgcg gttcctgaga
     5881 tttttggtta ccaatggcct gaatctgtct ccacatggct atggaaagtc tataattatt
     5941 cgatgggtgt tgtagggtta ctcgtttcag caaccactgc tcgctgcttg gctgattcga
     6001 tgaatcgtcg cttaccgaaa aacaaagtaa tcaacaccac ctctgtgatg ctggcctctg
     6061 tggtaagttt cctgttgtta agcgtctctc cgattgaagg tggattttca acggaatata
     6121 tggggacaaa gggactactg gcttcctttg tcgctgcatt tttaacagtt aatttatata
     6181 agttttgtgt gctgaaagat attaccatta aaatgcctaa agaagttcct ggaacgattt
     6241 cgcaaacctt ccgagatatt tttcctttct ctttttcagt atttgcggcc gttttaattg
     6301 atttaggcat tcgacaaatc ttccaaatct catttgcaga agcaatcatt acggtgctgc
     6361 agccgctatt tacagcagct gacgggtact taggaatcgc tattatctgg ggagcgatgg
     6421 cgctgttttg gtttgtcggt gttcatggac cgtcgatcgt ggagccggca attgcggcaa
     6481 ttatctatgc caatgtggaa accaatctgc aattgttcaa ttccggtgaa caagcttcta
     6541 aagtactgac ggtgggttta gggaattttg taggaactat gggaggaact ggggcaacat
     6601 tggttgttcc ttttatcttc ctactctttg ctaagtctaa gcagttgaag gcagttggga
     6661 aagcttcctt tattccagta agtttcgctg tcaacgaacc attgctattt gcggcaccca
     6721 tgattttgaa cccttatttt tttattccat ttttgttaac acctgttgta aacgtttgcc
     6781 tgttcaagtt ttttgtcgat gtattgggca tgaacagctt tatgtacgtt ttgccttggg
     6841 caactccagc accgatcggt ttggttctag gaacgggcat gggtatctta gcttttgttc
     6901 tagcagcggt tctaatcgtc gttgacttcc tgatctatct accattttgc aaagcatacg
     6961 atgctgtctt agtagagaaa gaagcacaaa aagcagaggt gatcgcaacc gaggaaggtg
     7021 cttctgccga tgtacgtgag gaagtacctg tggcagcgag tcaagttatc agcgatgcaa
     7081 ataaagaaat caatgtgttg gtactttgtg ctggcgcagg gacaagtggg atgttagcta
     7141 atgctttaaa aaaaggtgca tctgaatcga aagcaccgat cagtgcaatg gcgggtgctt
     7201 atggttctca ttacgaaatg ctgcaggatt ttgatatggt ggtcttagca ccgcaagtca
     7261 attcttacta tgatgatatc aaagaagaca ccgatcgctt aggaatcaag ctggttgcga
     7321 caaaaggagc agaatatatc ggattaacga gagatcctga aaaagctgtt cagtttattc
     7381 tgaaccaatt tcaataaagg aggagccata atgaaaatgg gaatttggtt gcgtaaagct
     7441 gttttatttg cagctgtgtt aggggctttg ggggctgttt caaaaactgt tgaagcatct
     7501 gccagcgaat ttattcgcgg agcagatatt tcgatcctgt cagatatgga aaaatctggt
     7561 gcaaaatatt atgaagacga tatttcaaaa gatgccttag agattttaaa aaataatggt
     7621 gtcaactatg ttcgactgcg tttgtggcag gatccttata atagtaatgg agaaagttat
     7681 ggagcaggga caaatgatct ggaaacgacg attgaccttg caaaacgagc aaaaaatctg
     7741 ggacttaagg tgctgctgga ttttcactac agtgattttt gggttgatcc tgggaaacag
     7801 aatcttccta aagcatggca aggtctgacc tttgaagaaa tgaatacggc attgtacgac
     7861 tatacaaaga acgttctgtc ggaaatgaag cagctggacg tttatcctga tatggttcag
     7921 atcggcaatg aactgaatag cggaatgctc tggccttatg gcaagtcttg gggagaaggc
     7981 gggggagaat ttgatcgatt ggctgctttt ttgaagtctg gtatccaagc tgttcgagac
     8041 actcaaccta aaacaactcc agtaatgctc cacttagctg atggtggcga tacaggagca
     8101 tttacttggt ggtttgatga aatcacgagt cgaggtgtgt catttgattt aattggagtg
     8161 tcgtattatc cgtattggca cggttcaatg aatgaacttc aaaccaatat gaatgcgatc
     8221 agccaacggt atggtaaaga agtgatcgta gttgaaacag cttatgggca tacaacagcg
     8281 aatgccgata ctatgccaaa tgcttttggt gaagccgaag cagctgcggg gggatatgaa
     8341 ccaacaccga caggacaagc agagtatttg ctggatcttg ccgatcggat tcaggcagta
     8401 ccgaataatc gcggggcggg cttcttctat tgggaacctc tttggtacaa cggaaatgtg
     8461 agttgggcaa cacaagcagg tatgaattat ctcagtgttc aatccactat gggaaacgaa
     8521 tgggacaacc aagcgatgtt tgattttgct ggaaacgcct tgccaagctt gcgtgcgttt
     8581 aaacaagcag gggcgcaaat aaatctagtt aaaaatgcaa gtttcgaaag cgatggacca
     8641 acgaccagtc cttccaattg gcaaacctgg tttcaacaag gaaccgctaa gggtacgatc
     8701 aaaacggaag cgggcacttt ttttggggac tacaaactaa ctttttggca ggatcaagca
     8761 tacgaggcat cagcttatca aaatgttagt ggcataagca aaggaactta cacattgagt
     8821 gcctatgtaa tgtctggggg agagtttgat actaatcagt tatatatcaa aaattatggt
     8881 ggagcggaac taaatactgc ggttgttaaa acaagtgaat ggacaaaaat tacaatcaaa
     8941 gacattccaa ttacaacagg tgtttgtgaa attggggttt atagcaaggc ttcaggaaat
     9001 gcttggacga acgttgattt tgtttcattg gtgaaacaat agactgcgga tcgtttcgat
     9061 tcaagatcgg aggttccctt cagccacatc gttagcgtag aaggcaggaa tcgaacctgc
     9121 acctcgcctt tgccaaaaat aaccagaaac gatttccagt catttttacc aaaacgagta
     9181 aagcaccaga cttcatctac actttgtatt atgctaaaga gagttcaacg atttcgtaag
     9241 aagcaatgaa aaaattgcaa aaagttgtgc agataacata aagtttgttt aagaaagggt
     9301 aaccttagaa ttccgtcgtg aatatgattt gagcacttag atggatagaa aaaagttctc
     9361 ggaaaacagt tcgtttttcg agaacttctt tttatgataa gaatgaaaca aattaactta
     9421 tattatagtc tcttttggta gggaaccatc ttatttttaa tgaaaatcaa gcattttagc
     9481 tattgaagaa tgttgattta tagggattcc ccctccatat tatgttggct ctttattccc
     9541 actccactgt tgctggtggt ttagaagtga tgtcatacac gatacggttg acgtgggcta
     9601 cttcgtttac gatacgtaca gagatttttt gcaagacttc ccatgggata cgggcaaagt
     9661 cagcggtcat tccgtcgatt gaagttacag cacggatacc gactgtgtag tcgtaagtac
     9721 gtccgtcacc cataacaccg acagagcgga tccctggaag aacagtgaaa tattgccaga
     9781 tatcacggtc aagtccagca gctgcgattt cttcacgcaa gatagcatca gattcgcgta
     9841 cgatttctag cttgtcttca gtgatttcgc caagaacacg aataccaaga ccaggaccag
     9901 ggaatggctg acgccaaacg attgaatcag gcatgcctaa ctgagtccct aattcacgga
     9961 cttcgtcttt gaataatgtg tttaatggtt cgatcaattc aaattgcata tcttctggca
    10021 gtccacctac attatggtga gatttgatcg tctgtgctgt ttctgtacca gattcgataa
    10081 cgtcagtata caacgttcct tgtgctagga aagagacgcc ttcttcacca gcaagttttg
    10141 ttgcttcgtc atcaaaaaca tatacgaatt cattcccaat gattttacgt ttttgttctg
    10201 ggtcagatac acctttcaac ttgtctaaga agcgatctct tgcattgact ttgatgatgt
    10261 tcaagccgaa ttttccgcct agactttcca ttacttgttc cgcttcgcct ttgcgtagca
    10321 gaccgtggtc aacaaagata gatgtcaatt ggtcaccgat cgctttttgt aaaagaaccc
    10381 caacgacact agaatcaacg ccacctgaaa gaccaagcaa gacttttttg tcaccaactt
    10441 gttcacggat tttttctact tgcatatcga taaagttatc cattgtccag ttaccttcac
    10501 agtgacaaac atcaaaggca aagtggcgca gcaattcgat tccgtattcg gaatgacgta
    10561 cttctgcatg gaattggata ccataaaaat tacgtgttgt gtcttggata gaagcaatcg
    10621 gacaatcttt gcttgttccg actgtttcaa agccttcagg aactttagtt accaagtcac
    10681 catggctcat ccatactgtt tgttgttttg gtgtttcttt aaacaagaca gcttgatcgc
    10741 ttgttacttc taacaaggct ttcccatatt cgcggttttt agcaggctct acggaaccgc
    10801 caagtttgta cgtgatcaat tgcataccat agcaaatccc taatacagga atacctaaat
    10861 tgaagatctc aggatcgatc ccaaatgcat tctcatcata tacactgttt ggtccgccag
    10921 aaagaatgat tccctttgga ttcatcgctt ttacttcttc tgcagtaatg cggtggctca
    10981 ataattcgga aaagactcca agatcacgaa tacgtcttgt gatcaattgg ttatactgac
    11041 taccgtagtc taatacgata atcttttcaa cgttcgtcat atcggcaacg tttgtcaaat
    11101 ttattacccc tatcccttta aattttatct caaaatagtc aaactatttt aaaatgctaa
    11161 tattttcgca tgaaaatcga atcgatgata tgattttcag ttttatggag aattatatcg
    11221 gctcttcctc gtgtaggcaa gatatattcc tgtaaatttt tcaaattcac atttttccat
    11281 acatcttttg ccattttaaa ggcttcttca cgatctccga tagcaaaacg ataataataa
    11341 ttctctggat tttggaaagc cgtatccagt aacgcaccaa aacgatccaa ataccatttt
    11401 tcgatcagca cgggatcggc atcgacatag acagaaaaat caaaaaagtc gctgacgtag
    11461 atttgctgat tcgcaggtaa ttgaagcgta ttgatcccct caacgatcaa gatatcaggc
    11521 tgctggatca attcgtactt gtctggaatg atatcataca catcatgtga gtaaacaggt
    11581 acttttatct cttcctgacc agattttaca cggttcaaga aatcgatcag catctccata
    11641 tcataacttt caggaaatcc tttacggtcc atgattccac gctcttttaa tatacgattt
    11701 ggataaagaa atccgtcagt cgtgatcagc tgaacgtttc ttcgtttgaa ggtacgagac
    11761 aaaatcgtct gcagcagtct ggctgttgta ctttttccaa cggctacact tccggcgatc
    11821 ccgataataa aaggcggtac ttctacgtat ttatgaagaa ataaaccttt actcaagaac
    11881 aaagattcaa attctttcat ataaagataa atcagatgag caagaggtac atagatatct
    11941 cgaacatcct gcatggaaat gcggtcattc aaacttttga tattatctag ttcatcttgt
    12001 gtcaaaggtg cttgcccatt acgataaaat tctcgccatt cttctcttgc cacacggtag
    12061 tagttcattg gatcattcat attagcctcc tgcgttctcc catcaatgga actattttaa
    12121 cacaaaataa acgcaaaccc caccacttaa gagagattgc cgatcttttt gtataatctt
    12181 tataatagaa agaaacggag tcttattctt gacttgcgca cggatacata aaaggataga
    12241 ctgttgtcat gaaagaaatg agaaggatgg aagacatgac aaatcattac tattctgaac
    12301 gcccagatac ggcgcacgat tttgaacaat gggcttttga gttaaaaggc agaacctttc
    12361 aatttgtaac agattctggt gtattttcac gtgaaacagt ggattatggt tcacgtgtcc
    12421 tgattgatgc tttcgattgg gaagagttac ctgatgaagg aaagatctta gatgtgggat
    12481 gcgggtatgg accgattggg ttagctttgg catttgcgac acagcggttt gttgaaatgg
    12541 tcgacattaa tgaacgagcg gtaagtctag cgcaaggaaa cgcaaagcgt aaccaaatcg
    12601 aacatgttga tatccatcaa tcgaatatct atgaagcagt tcatgaagaa acctatgcag
    12661 ctatcgtaag caatccgccg atccgtgctg gaaaaaaagt tgtacatgaa attttgacag
    12721 gcgcatatcc tcggttgaag aagggcggta cactgacaat tgtgatccag aagaaacaag
    12781 gagcgccgag tgcgcaaaag aaaatggaag aaacatttgg taatgcagaa atcgtcacaa
    12841 aagataaagg atattatatc atcaaaagtg tgaaagaata actagctgaa aaatagtgga
    12901 gcgacgaaag agtggaactg ggcgtgcatg tacgggtacg tatcagttct ttttttgttg
    12961 agaagttttt aaaaacaaag aactattttt tcttcaaaaa gaaatagtag gaacagagtc
    13021 gttgtttttt acgataaaat aaataagata cagaacttgt atagaaggct gccaagtcgg
    13081 tagataaagg aggtttttat caaaaaacat actttccttt tttctctatt cttttcttgg
    13141 gggttttggt acactgatag ggaatttaga tagaagggag ccaataggat gaagctgacc
    13201 atcagagata tcgcagaaat ggcaggcgta tcagtaacaa ctgtctcaca gattttgaat
    13261 aacaaaggaa gcagatttag tgataagaca agaaaaaaag tgttgtcgat cgtgaatgaa
    13321 catcattata agcccgatta tttcgcttca aatgtgatca atcgacattc aaagaccatc
    13381 ggaatgatcg tccctgatgt cactgatttc ttcttctcca aactgatcga aggtgtagag
    13441 agctatttga atcctttggg ctatgtgatc atgttatgca attctcgtca tagccaagaa
    13501 aatgagatca agtatttaca agagctttcc caccgctcag tggacggaat cttgctggcc
    13561 acaccaaata ttttacctga gaaatatgca ttagacagcg gtttttatca aaaaatgcca
    13621 atcattttaa tcgatcgcgg attgaatcgt cgggataacg gacggcttat cgtaaaggaa
    13681 tatgagggcg cctatcaagc tgtttcgctg tttattgaaa atggccatac gaagatcggc
    13741 atgctgaagg aaaccactgg atactatcaa ttagaagaac gtttcaacgg atatcgtcat
    13801 gcgctgaaag atcatggcct gccttttaat gggaaatttg ttgaacatgg agagttgact
    13861 gtccaaggtg gatatactgc atcaaaaaaa ttactcaaac ataaagatat cacggctatt
    13921 ttttgcggga atgatgccat ggcgatcgga tgctatcagg cgatcgatga attgggcaaa
    13981 aaaatcccag aagatatctc agtcatagga ttcgatggat taaagctgtc agaatacatg
    14041 attccgcaat tgacgacagt gcagcagcca agttttgata ttggatttta tgctgcacgt
    14101 ttcctgatcg atacgatcga atttcctcaa agaaaagttc cgaacaaagt gttcgaaaca
    14161 aaattaatta ttcgtgagag cgtaaaagta ttgacaaagg gctaactcgg cggtatattg
    14221 cttatgtaag catttacaaa ggctagttag tgcttttttt atttttagta aaccagttta
    14281 ctaaagtggt ttacctttcc taaaaataat gtataatacc gaatgaagac aaggaggatc
    14341 aggcaatgcg aatggtggat ctgattgaaa aaaagaggga tggaaacgag ctgtcgaaag
    14401 aggaaatcga gtacatcgtc acgaactata cgaatggaaa gattccagat tatcaagtca
    14461 gtgcattatt gatggcaatt ttttatcaag acatgacaaa tgaagaaatc acgaacttaa
    14521 cattggcgat tgctaattct ggtgatgtca tcgatttgag ttctcttgaa ggaataaagg
    14581 tagataagca ttcgacaggt ggcgttggtg acacgaccac attgatttta gcccccctag
    14641 tagctagtgt cggagtgaca gtggctaaaa tgtctggacg cggtctaggt tacacaggtg
    14701 gtacactaga caagctagaa gcaattcccg gatttcagat cgagctttcg gatgaagaat
    14761 ttgtacgcat tgttaatgaa agtaaagtag cggtaattgg tcaatcagga aacttggcac
    14821 cggcggacaa aaaattgtat gccttgcgtg acgtcacagc gaccgttgat tctcttccgc
    14881 tgattgccag ttcgattatg agtaaaaaaa tcgcggcagg agcagatgca atcgttttgg
    14941 atgtgaccac tggagacggt gctttcatga agaacatcga agatgcaaga cgtctggcaa
    15001 agaccatgac gagcattggg aaactggcta accgagaaac agtggcggtt atctcagata
    15061 tgtcagaacc tcttggcgaa gctatcggaa atagtctaga agttgtcgag gcaatcgaaa
    15121 cattacaagg aaatggtcca gaagatctag tagaaatgtg ctacgcctta ggaagccaaa
    15181 tggttgttct tgctggcaaa gctaagacaa ccgatgaagc aagaacgttg cttcaagaag
    15241 ctctggaatc aggtaaagcg ttagcaaaat tcaaagaaat gatccaaaat caagggggag
    15301 acccagcgat cgtggaacat ccagaacgaa ttttgactgc acgctacaca atggagcttc
    15361 ctgctaaaca aagcggagtt gtgtcaaaaa tcgtggcaaa tgaattagga attgctgcaa
    15421 tgatgctagg tgctgggcgt aaaaccaaag aagaagatat tgatcacgct gttggattga
    15481 aactgcataa aaaaattggt gacactgtga caaaaggtga gtcactattg acgatctata
    15541 gtaacgacga agaaatcact tctgtcattg aattattata taaaaatatt gaaattggtg
    15601 aatcggcaat gaagccaacg ttgattcatg acatcatcac agaataaagg aaaaaagcgg
    15661 actttaccgt tttaaagaaa aggggaaaag aaatggaact aaaccgaatg atcgaccata
    15721 ctattttgaa agcagacgca tctaaagaag ctgtgatgca aattatcgaa gaagcaaaaa
    15781 aataccattt ttattctgtc tgcatcaatc cagcatgggt tgcgctagcg aaagaacaat
    15841 tacaaggtga gccagtagcc gtatgtacag taatcgggtt tcctctagga gcaaacacac
    15901 ccgagacaaa agcatttgaa acaacagatg ccatcaataa cggtgcagat gaagtcgata
    15961 tggtcatcaa tatcggtgct ttgaaatcaa aggatgatca acaagtccaa aaagatatcg
    16021 aagcagttgt tgaagcagca aaagatcgtg cgcttgtcaa agttattatt gaaacatcgt
    16081 tattagatag agaagaaatc atccgagctt gcgaaatagc aaaagctgca ggcgctgatt
    16141 ttgtcaaaac atcaactggt ttctcaacag gcggagcaaa ggtagaggat gtccgtttga
    16201 tgcgtgaaac tgtaggacca gaaatgggtg tcaaagcttc cggcggtatc cataacgaag
    16261 aagaagcaat ggcaatgatc gaagctggtg ctacaagaat cggaacaagc gcaggagtag
    16321 caatcgtatc tggttcaact ggtgaaggct attaattttt tcaaaggaga agctcatggc
    16381 agaactcaaa caagaatgga tcaacacagc tattgaagcg ttagataaag cctatgttcc
    16441 ttattcccat tttccggtag gcgcttgtct cgtaacggaa tcaggcaaga tttatcaagg
    16501 catcaacatt gagaatgcat catttggatt aacgaattgt gccgagcgca cagctttttt
    16561 caaagcagta tctgaaggag aaaaaacatt tacgcatcta gtcgtagcgg ggcacacccc
    16621 cgatcctatc tctccttgtg gtgcatgtcg acaagtaatg gcagaattct gttcccctga
    16681 tatgccggtt acgctagtag gagacaatgg tgtgacgaag gcaacgaccg ttcgtgcact
    16741 gctgccttat gcatttactg aaaaagatct ataactcatg ttcattcagt cagctgactg
    16801 ttgaaaaata aaacaattat tttaggaggc tttacagaaa tgaaaaaagc aaaattattt
    16861 ggtttaggtg ctgtcgcact agcagcaggg ttatttttag gtgcatgcgg caacaacggc
    16921 tctactgatt catccggtgg caaaagctca tcagacacaa caactgcagc attgatcaca
    16981 gacacaggtg gtgtagacga ccgttcattc aaccagtcag cttgggaagg actagaaaaa
    17041 tgggggaaag atcaaggact ttcacgagga aatgatggct tccaatattt ccaatcttca
    17101 aatgaatcag attacatccc aaatattgac caagcattga atgctggatt caaaacgatt
    17161 tttggtattg gttataaact aaaaccagcg atccaagaac aagcaacaaa caatacaggt
    17221 acgaactttg ttattatcga tgatgtcatc gatggattag ataacgttgt atctgcaact
    17281 ttcaaagaca acgaagcttc ttatcttgca ggtgttgcag cagcttatac gactgaaaca
    17341 aacgtagtcg gctttatcgg cggtgtaaaa ggtgaagtaa tcgatcgttt tgatgctgga
    17401 ttcaaagccg gtgttgatgc aggtgcgaaa gaattaggaa aagaaatcaa agtattgaat
    17461 caatatgctg gtgacttctc tgctccagat aaaggacgtt caattgctca aggtatgtat
    17521 gcacaaaacg cagatatcat cttccatgct tcaggcggta caggtaacgg tgtattccaa
    17581 gaagcaaaat cattgaacga atcaggcgac aaaaaagttt gggttatcgg tgttgaccgc
    17641 gaccaatcag acgaaggcga atacacatta aacggcgaaa agaaaaactt cacattgaca
    17701 tctacactga aagcagttgg aacagtagta gaagatttgg cacaaaaatc agcggacggt
    17761 aaattccctg gcggcgaaca cactgtttat ggattgaaag aagacggtgt tggtctaaca
    17821 gaaggacaac tttctgacga agcgaaaaaa gcagttgatg aagcaaaaga aaaaatcatt
    17881 tcaggtgacg taaaagttcc agaaactcct gaagaaaact aaaaccattt ctatagcctt
    17941 gttagacaca tgtttgacaa ggctatggtt tgattagaac aagaattgtt catttatcca
    18001 agctgccttg tgacagtttg gataagggaa caactccctg aagtaatgtt tgtttcaaaa
    18061 gaagaacgct cactaagcgt gaatcttagt caatcgttgc cctagataga aatcgttagg
    18121 atgtgaaaaa aaggtggttc aagaaaatat tgtcatagaa atgcagaata ttaccaaaga
    18181 atttggaaca ttcaaggcaa atgacaacat caatttacaa gtaaaggcag gagaaattca
    18241 tgcgttgctt ggagaaaacg gtgctggcaa atctacattg atgaacgtgc tttccggatt
    18301 attagagccg acatcaggga aaattttgat gcgtgggaaa gaagtaaaga tcacaagtcc
    18361 gaccaaagcc aaccaattag ggattgggat ggtccatcag cactttatgc ttgttgatgc
    18421 ctttactgta acagaaaaca tcgtgttggg aagcgagcct agtcgcgcag ggatgcttga
    18481 ccataaaaaa gcacgaaaag agatccaaaa agtttctgaa cagtatggat tgtcagtcaa
    18541 cccagatgct tacgttcgtg atatttcagt tggaatggaa caacgggtag aaattttaaa
    18601 aacactttac cgaggagcag atgtactgat ttttgatgag ccgacagctg tattgacccc
    18661 tcaagaaatt gatgaattaa tcgtgatcat gaaggaatta gtcaaagaag gcaagtcgat
    18721 cattttgatt acgcataagt tagatgaaat caaagcagta gctgaccgct gtacagttat
    18781 ccgccgtgga aaaggaatcg gtacagtcaa cgttaaagac gtaacctcac aacaattagc
    18841 cgatatgatg gttggaagaa cggtttcatt caaaacgatg aaaaaagaag cgaagcctca
    18901 agaagtcgtt ttgtctattg aaaatctagt ggtgaaagaa aatcgtggat tagaagccgt
    18961 gaaaaacttg aacttagagg ttcgtgctgg cgaagtactt ggtatcgctg gaatcgatgg
    19021 aaacgggcag tcggagttga tccaagcttt gactggtttg cgaaaggcag aaagcggaca
    19081 tatcaagcta aaaggggaag acatcactaa taaaaaaccc cgaaaaatca ctgaacatgg
    19141 tgtaggacac gtgccagaag accgtcataa atacgggtta gtcctagata tgacattatc
    19201 tgaaaatatc gcactacaaa cctatcatca aaaaccttac agtaaaaacg gcatgctgaa
    19261 ttattcagta atgaatgaac atgccagaga attgatcgaa gaatatgatg ttcgaacaac
    19321 gaatgaactt gttcccgcaa aagctttatc aggcggaaat cagcaaaaag caattatcgc
    19381 tcgtgaagtc gaccgagatc ctgatctgtt gattgttgca aatccaactc gtggactgga
    19441 tgtaggtgcg atcgaattta ttcataaacg tctgatcgaa caaagggaca aatacaaagc
    19501 agtgttactg atcagtttcg aattagaaga aattttaaat gtttcggatc gtattgctgt
    19561 tatccatgaa ggagaaattg tcggtatcgt tgatccgaaa gaaacatctg aaaatgaatt
    19621 aggtctattg atggcgggct ataccttaga agaagcacga aaagagttag aaaagggggc
    19681 tggcgcacat gaatgatcgc agcgaaaaat tccgtaattt attagtgcct gtactttcag
    19741 ttgttttagg attgatctta ggcgcgatat tgatggctgc ttttggtttt cagcccatcc
    19801 aaggttattc ttctatgcta gatgcagccc ttggttcgca aagagggata ggtgaaacgt
    19861 taaggcaagc aacgccgttg atttttacag cattaggttt ttctgtagct aactcagctg
    19921 ggtttttcaa tattgggctt tctggacaag cactttgcgg gtggatcgtc agtgtctggg
    19981 tggcacttgc gtttccagat ctgccaaaag ttgttttgct tccgttatgc gtgatcttag
    20041 gtgccttagc tggtgcgatg gcagcggctg ttccaggttt acttcgagca ttttttggaa
    20101 caagtgaagt gatcgtaaca atcatgatga actatatctt attatacgta agcacatttg
    20161 ctttacatga agtgatgcct gaaagctatc gctcaagttt ggactcttcc aatatgatca
    20221 gtcaaaatgc ctcactgcgg ttaccatggt taacacaagc ttttggcggc tctcgcgtaa
    20281 actcaggatt atttttagca atcatcgcgc ttattttcgt ttggctgctg atgaagaaaa
    20341 cgactttagg atttgaaatc cgttctgttg gattgaatcc ttttgcttct gagtatgcag
    20401 gaatgagtag taaacgtacg atcgttttgt cgatggtcat ttctggcgct ttggcagggc
    20461 ttggcggtgt ggttgaagga ttaggaacgt atcaaaactt ctttgttcaa acgacttcgc
    20521 tatctattgg atttgacgga atggctgttt ccttgcttgg ttctggatca gcaatcggta
    20581 ttcttttatc agcgatcttg ttcagtattt tgaagatcgg tggacttggc atgcagacag
    20641 gagcaggtgt tccgtttgag atcgtcaatg tatcgattgc tttgattatc ttctttgtcg
    20701 ggatcaactt cctgattcgt ttcgtcatgg ctaaagtctt ccaagggaaa aaacaagaag
    20761 agatcgttgc gacaatagaa tcgaaaccaa cgaatcaaaa ccatgaaggg ggcgaactat
    20821 aatgtcgact atcgaattaa ttgccttgat ccttacttct acactggttt attcaacacc
    20881 gctaattcta acgtcactag gcggaacttt ttctgaacga agcgggatcg taaacgtagg
    20941 gctagaggga atcatggtca tgggtgcttt tagttccatc gttttcaact tgacgatgag
    21001 tccatcactt ggttctatga caccatggat cgctatttta gtgggtggcg tcgtaggtat
    21061 cctattttct ctgattcacg cagtagcaac agtcagtttg cgtgctgacc atattatcag
    21121 cgggacagta attaatctga tggcacctgc tttaggtgtt ttcttgatca aagcatggta
    21181 tggtaaaggt caaactgata acatcacgca gaatctgggg tatttttctt tcccaggatt
    21241 agcagatatt ccggtcattg gaccaatttt ctttaaaaat acattcttac ctgcttattg
    21301 tgctattttg attgctgtcc tagcttgggt gatcatcttc aagacgaaat tcggcttgcg
    21361 cctccgttca gttggtgaaa atcctcaagc tgccgacaca ttaggtatca atgtatatgc
    21421 tatgcgttat tcaggcgtac tgatttctgg cttgttaggc ggtatgggcg gtgctgtttt
    21481 tgctcagtcg atttccggta acttttcagc tggtacgatc gttggacaag ggttcatctc
    21541 catggctgct atgatctttg gtaagtggaa tcctttaggg gcaatgggtg cctcattgtt
    21601 ctttggtttt gctcaaagct tgagtatcat tggcgcacaa ttaccagtta tttcttcgat
    21661 tccgccagta ttgctgcaaa ttgctccata tttgctgaca atcattgtgc tggttgtttt
    21721 cttagggaaa gcttctggtc caaaagcaaa cgggaagaac tatatcaaat caaaataatc
    21781 ttttagcgaa taaaaagggt gggttacggg aagtgttttt cgtctcgccc tttttgtttt
    21841 aaaggcaata aaaataaaaa gaaagaagga ataatcatgt ttaaacgcgt acatctgatc
    21901 gtcatggatt cagtaggaat tggagaagca ccagatgccg ataagttcgg tgatgttggt
    21961 tctgatacat taggacacat tgcgaaagaa gcaggattga acgttccaca tttagagcag
    22021 cttggtttag gaacgatccg tcctcttgaa ggtgtggaat cagtgaaaga tcatcaaggc
    22081 tatgcgacga aattagaaga agtttctgta gggaaagata cgatgacagg ccactgggaa
    22141 atcatgggat tgaatatcaa aactccattt cgtgtgtttc ctaatggatt ccctgatgat
    22201 ctgatcaaac aaatcgaaga attttctggc cgtaaagtcg tatgtaacaa accttatagc
    22261 ggaacagctg ttattgacga ttacggtgaa caccaaatga aaacaggcga tttaattgta
    22321 tatacttctg ccgatcctgt ccttcagatc gcggcacatg aagaaatcat tcctttagaa
    22381 gaattatatc ggatttgcca attcgttcgt gatataacaa aagatgatcc gtatatgatt
    22441 ggccgaatca ttgcacgtcc gtacgtgggt gaaccaggag acttcacacg aacaagcaat
    22501 cgccatgatt atgcgttgaa tccattcgga cacacagtcc ttgattcttt gaaagaagca
    22561 ggaaaggacg tgattgctgt tggaaagatc aacgatattt tcaacggaca aggaatcact
    22621 gaatctgtcc gcacgaaaag caatatggat ggcgttgacc agctgctgaa agttatgaaa
    22681 aacgattttt caggaatcag ctttaccaat ttagtagatt ttgatgcatt atacggtcat
    22741 cgtcgggatg tagtcggcta tgcgcatgcg attgaagaat ttgacttacg aattccagaa
    22801 ttgcttgaag cgatggaaga agacgacttg ctaatgatta cagccgatca cggaaacgat
    22861 ccgacgttta ctggtaccga tcatacgcga gaatatgtac cgttattggt ttacagtaaa
    22921 aaaatgaaag aacaaggggc attgcctcaa ggatattatt cagatatttc agcaacgatt
    22981 gctgagaact ttagtgtttc ttcgactgaa aatggcgaaa gcttcttgaa attattaaaa
    23041 taatcttttt aaataggaga aagcaaatga ccaagttaag cgagatgtta caagaaacga
    23101 cccagtttat ccttgataaa ggagtaaaag aaattgattt tggtttgatt ctaggttcag
    23161 gattaggtga attagcagat gaaatcgaag atgctgtaat gatttcttat gatgagatcc
    23221 cgttctttcc tgtttcgaca gtagttgggc atgcaggaaa gctagtttat gggtctctgg
    23281 caggcaagaa agtgttagcc atgcaaggga gattccacta ctatgaaggt cattccatgc
    23341 aaacagtgac ctatccagta cgtgtgatgg ccggactagg ggcccattca gtcattgtga
    23401 ctaatgcatg tggtggtgtc aacgaatcat ttgtaccagg agatttgatg ctgattaccg
    23461 atcatatcaa ttttactggt gaaaatcctt tgatcggtcc gaatgaagat gcaatgggcc
    23521 ctagatttcc agatatgagt caagcttata cgcctgacta tcaagcaaca gcaaaagcag
    23581 cagcagaaaa actaggtttg gatcttaaag aaggcgtata tatgggctat tctggaccaa
    23641 cttatgaaac gcctgcggag atccgaatgt ctcggacaat aggagccgat gcagtaggga
    23701 tgtcgactgt tccagaagtc atcgtagcag ctcacagcgg attaaaagtg ttaggaatct
    23761 cttgtattac caacttagca gcaggtatgc aagcgaatct gagccatgaa gaagttgtag
    23821 aaacaactca gagagtcaaa caatctttta aagcattggt taaagaaaca ttgacattat
    23881 tgtaatagga ggaacaataa aatgagtgtg catattgaag cgaaagaagg cgagatcgcc
    23941 gataagattt tacttccggg agatcctttg cgagctaaat atattgcgga gacttttttg
    24001 gaagatccag tttgttacaa tcaggtacgc ggtatgttag gctttaccgg caaatataaa
    24061 ggagaacgta tttctgttca aggaacagga atgggaatgc catcagccac catctatgcc
    24121 catgaattga tccagtcgta tggtgtaaag aaattgattc gagtaggtac atgcggtgca
    24181 ttgtcaaaag acgtccatgt tcgtgattta gtactagcac aaggtgcagc gaccagttct
    24241 tcgatgatcg aaaaaaattt ccaagccttt cattttcctc caatcagtga tttcaatcta
    24301 ttactgaaag catatgagat agctaaagaa aagggctata cggttcatgt aggaaatgtt
    24361 ctttcggaag attctttcta caaagatgat ttgacagaga cgttccaatt agctgaactt
    24421 ggcgttttag gagtggaaat ggaagcagcg gctctttatt acttaggcgc gaaataccat
    24481 gtgcagacat taagtttgat gaccgttagc gaccatttga tcaccggtga agaaacaaca
    24541 gctgcagaac gtcaatctac gttcaatgaa atgatcgaag taggattgga aacagcgata
    24601 gcagaataag aaggaaatga aaaagctgcc ctgcattaag taataagaac atccaagcaa
    24661 aaatagctcc tcatgttttt gacttggctg gacttattat tgcaaatgta agcttttgaa
    24721 caccgtttac caagaggttg tgaatctgcc gtttaactct gagtcacaag gaacaatttc
    24781 aaaaaacagc ttttcatgtt tgatgaatat tgtgacttgt gaccgagaag ttggcaggag
    24841 aaaaccgttt atacgaaaaa agggactgtg acaaatcgtg tcacagtccc ttttttatag
    24901 tgttttttat aggtagtatt ttttagcaac tgttaagtca tcatttaatt cataaacaag
    24961 tggttgacct gttggaattt caagatccat gatgtcttcg tcagagatac cttcgatatg
    25021 tttagctagg gcgcgtaatg agttaccatg tgcagctact aaaacagttt tgttgtctag
    25081 taatgctgga gcaatttcat cttgccagaa tggcaaagcg cgttctaatg tcactttcaa
    25141 gttttctcca ccaggaatat cgcgtttgtc taacattgcg taacgacggt catttgctgc
    25201 agaaccttcg tcagtttctt ccattaatgg aggaagagta tcgtaagaac gacgccagat
    25261 gtgcacttgt tcgtcgccgt atttttcagc tgtttctttt ttgttcaaac cttgtaattt
    25321 accatagtga cgttcgttta agcgccatga tttgatttgt ggtacccaaa gttgatctga
    25381 atattctaag atcaagttac atgttttgat tgcacgagtt aaaacagaag tgtaagcaac
    25441 atcaaattcg attccagctt ctttgatttt gcgtccgcct tcttttgctt cttcgattcc
    25501 ttctggtgct agatttacat cagcccaacc agtaaattgg ttcaatgcat tccattcact
    25561 aagaccgtga cgagaaaaaa ctaattttgg catgtttagc tctcctttta agataggttt
    25621 atatttctga aattttcagt tctgttttat tgtacaattt tttggaagag atttccatcg
    25681 aaaaattaga taagattcta tgagaaaaca agtagagaat ataaaaaaac gaacagtgcc
    25741 cacgcaactg ttcgttttta ggtattagat gcataattgc tcgatttctt tttcaggtaa
    25801 attgatatat ttataacatg ttccaactga aacaccgcat tttgaagaaa taaattggat
    25861 cgtttctttt ttttcataat agagacgtcg gatttttttg atcgtttttg catcgatttt
    25921 tggtcttccg ccgatttttc cttttttacg agcttcatct aatccaacta atgtacgttc
    25981 tttgatcaat gcgcactcca tgtttgctag gttttccatt aattggaaat agatttttcc
    26041 catttcgcgc gtatcgattc cttcatcaag actaatcaag tgcagcccgc gatctttgaa
    26101 taattgcgtg agttctgtta attgccgcgt agatttgcct aatcgatgaa gttggcaaat
    26161 gactagtgca tcgccttgtt gcatgtcagc gatcactgac tccaaatgaa cagcagtaga
    26221 atcattttgt tccacttcat atgtctcatg gaaaatttct tgacaaccaa agtcagataa
    26281 ttgatcaacc tgagttgtta aatcaacgtc atttatggtt gtgcgtgcat aaccaataat
    26341 tttcatggtg attcctttct agcagtgatt ttctaactgg atataacagt aaaaatgtag
    26401 cataaagtac gtcaaaaaga tagttttttc gcaaattttc cgctgtcttt ttttattgga
    26461 tgatcaagca gtttttcttt atttacgacg gattgcgtca agaatctcaa cgcctttttc
    26521 tgagccgaca actactttag aaaccatgtt taggaaaagc ccatgttcta ctaccccaac
    26581 aagttcatct aaataggtag ctaagctttc tggctgttcg atgacttcta gatgcagatc
    26641 aatgatgtaa tgaccaccat ctgtggttaa gacttctccg ttttcgtctg tacgcaagac
    26701 gggttggaat cttttttctt caaaaatatg aagtagctgt tgggagccat aagggatcac
    26761 ttcaactggg agtgggaact tcccaagttt atcgactaat ttagaagaat caacgatcca
    26821 aatcgtttct tttgagtacg tagcgactat tttctcgaaa agcaaagcag cgccgccgcc
    26881 tttgatccct tgaaaatcag cactgatttc atcagcaccg tctattgtca aatcgactac
    26941 ggggacttca tcaatactct ttaacggtat tcctaatttt tccgcttgtt cttttgtcgc
    27001 atttgaagta gtaactcctg tgatacgtaa accttcttca cgcatacggc gaccaatctc
    27061 ttctaccata taataggcgg ttgaaccggt cccgagacct acgatcatcc cgtcctttac
    27121 gaatttcgcg gcttcaatgc cagctaattg ttttaaattc atctttgcgt ccaccttttt
    27181 ttagtttgag catcgtgctt tcctcactat tttagcgtga tttacggtaa aaagatacca
    27241 ttctttttta ttccaataaa gctttttcaa aaaaaattgc atctcttgtt gacatgtttt
    27301 ttcattcgtg atattctaac aaaggtgctt tatctacagg tttctgtgaa gaaacgtgct
    27361 gactgtttat caaagcatat ttgataagga tgcagacatt gcatttattt tttatcacaa
    27421 aaaaagaacc acctggatgt gtggaactag acgcaaatcg tgggaaggag gaaacaagga
    27481 atgcctacaa ttaaccaatt agtacgtaaa cctcgtaaat caaaggtgga aaaatctaat
    27541 tcacctgctt tgaacaaagg atataacagc tttaagaaaa ctcaaactaa cgtaaactca
    27601 cctcaaaaac gtggggtagc aactcgtgtg ggtacaatga cacctaaaaa accgaactca
    27661 gctttacgta aatatgcacg tgttcgtttg tcaaacttaa ttgaagttac tgcttatatc
    27721 ccagggattg gtcataactt acaagaacac agtgttgtat tattacgcgg tggtcgtgta
    27781 aaagacttac caggggtacg ttaccatatc gttcgcggtg ctttagatac tgctggtgtt
    27841 aacgaccgta aacaaagccg ttctaaatac ggaactaaaa aacctaaagc ttaattttaa
    27901 ttattaaaca aatgaaactt gcttctacta tatagaagta attactatga acaatatttc
    27961 ggaaggagga gttacggatg ccacgtaaag gtcctgttgc aaaacgtgat gttttaccag
    28021 atcctattta taactcaaaa ttagtaactc gcttgatcaa ccgtgtaatg gttgacggaa
    28081 aacgcggtat tgcagctaac attatctata atgcctttga cttgatcaaa gaatctacag
    28141 gtaacgatcc attagaagtg tttgaacaag caatgaaaaa cgttcaacct gttcttgaag
    28201 ttaaagcacg tcgtgttggg ggttcaaact accaagtacc agttgaagtt cgccctgaac
    28261 gccgcgttac tctaggtctt cgttgggtag taaactacgc tcgcttgcgc ggtgaacaca
    28321 caatggaaga acgcttagca aaagaaatca tggatgctgc aaacaacaca ggtgcttctg
    28381 ttaaaaaacg tgaagacact cataaaatgg ctgatgccaa ccgtgcgttt gctcactatc
    28441 gctggtaaga ttctctctgt ttggtcggct tttataagtc gacctcagat agtgtcctta
    28501 tttttagcaa ctagatagta aagagaggag aaacaaggag aatgaccaga gaattttctt
    28561 tacaaaacac tcgtaatatc ggtatcatgg cccacatcga tgctggtaaa acgactgcta
    28621 ctgaacgtat cttgtactat actggtaaaa tccataaaat tggtgaaact catgaaggag
    28681 cttcacaaat ggactggatg gaacaagaac aagaacgtgg tattacgatc acttctgctg
    28741 ctactacagc acaatggaag ggtcaccgaa tcaacatcat cgacacacca ggacacgtag
    28801 acttcacagt tgaagttgaa cgttcattgc gtgtacttga tggtgctgta actgttcttg
    28861 atgcacaatc aggtgttgaa cctcaaactg aaacagtttg gcgtcaagca acaacttatg
    28921 gtgtacctcg tatcgtattt gcaaacaaaa tggacaagat cggtgctgac ttcttatatt
    28981 cagtatcaac tttacatgac cgtttgcaag caaacgctca cccaatccaa ttaccaattg
    29041 gtgcggaaga cgacttcaca ggaattatcg accttgtaac aatgaaggct gaaatgtata
    29101 caaacgatct tggaacagag atcgaagaaa cagaaattcc tgaagaatac cgcgaattag
    29161 ctgaagaatg gcgcgaaaaa ttggttgaag ctgtagctga aactgatgaa gaacttactt
    29221 tgaaatactt agaaggtgaa gaaatcactg aagctgagtt aaaagaaggt atccgccgcg
    29281 caacagttaa cgtagaattc tacccagtac tatgtggttc agcatttaag aacaaagggg
    29341 ttcaattact attggacgct gttcttgatt acttgccatc accgttagat atcccagcta
    29401 tcaaagggat tgaccctaaa actgatgaag aagtagaacg tcctgcagac gacagcgctc
    29461 ctttctcagc tttagcattt aaagttatga ctgacccatt cgtaggtcgt ttaactttct
    29521 tccgtgtgta ctctggtgtt ttacaatcag gttcatacgt acaaaacgca acaaaaggca
    29581 aacgtgaacg tgttggtcgt atcctacaga tgcatgccaa ctctcgttca gaaatctctg
    29641 aagtttacgc tggagatatc gctgctgctg ttggtttgaa agacacaact actggggaca
    29701 cactatgtga tgaaaagagt ctagttattc ttgaatccat ggaattccca gaaccagtta
    29761 tccaagtcgc tattgaacct aaatcaaaag ctgaccaaga taaaatgggt gtggctttac
    29821 aaaaactatc tgaagaagat cctactttcc gtgctgaaac aaacgtggaa acaggcgaaa
    29881 caatcatcgc tggtatgggt gaacttcact tagatatcat cgttgaccgt atgcgtcgtg
    29941 aattcaaagt tgaagctaac gtaggtgcgc ctcaagtatc ttaccgtgaa actttccgtg
    30001 ctggcactca agccgaaggt aagtttgtac gtcagtctgg tggtaaaggt caatacggtc
    30061 acgtatggat tgaatttaca ccaaatgaag aaggtgctgg tttcgaattt gaaaatgcga
    30121 tcgtcggtgg ggttgttcct cgtgaataca ttccagctgt tgaaactggt ttgaaagatg
    30181 ctatggaaaa tggtgtctta gctggctatc cattagtaga tatcaaagct aaactttacg
    30241 atggttcata ccatgatgtc gattcaaatg aaactgcctt ccgtgttgct gcttctatgg
    30301 cattacgtgc agcagctaag aaagctaatc cagtaatcct tgaaccaatc atggctgtag
    30361 aagttgttat ccctgaagat tatttaggtg atgttatggg tcacgtaact gctcgtcgtg
    30421 gacgtgttga aggtatggaa gctcgcgctg gcggccaaca agttgttcgt gcgatggttc
    30481 cacttgctga aatgtttggt tatgctacaa ctcttcgttc tgctactcaa ggtcgtggta
    30541 catttacaat gacatttgac cactacgaag atgtaccaaa atcagtacaa gaagaaatca
    30601 ttaagaaaaa cggcggaaaa gctgaataat ttcttgaaac cggatagtcc tgcaaagaga
    30661 ttatccaaag agactatatt tttaatatag tttcgtgtaa aataattatc gatgatatgg
    30721 gcatgtggtg taccttgtgt acatcatata cctatcagac aaataaaaaa tatattgtat
    30781 ttttaggagg acattttaaa atggcaaaag aaaaatttga ccgttctaaa ccacacgtta
    30841 acattggtac tatcggacac gttgaccatg gtaaaactac attaacagct gcaatcacaa
    30901 ctgtactatc taagaaaaac ggcggccaag ctatggctta cgatcaaatc gatggtgctc
    30961 cagaagaacg cgaacgcgga atcacaatct caacagctca cgttgagtat gaaacagata
    31021 cacgccacta cgctcacgtt gactgcccag gacacgcgga ttacgttaaa aacatgatca
    31081 ctggtgctgc tcaaatggac ggagctatct tggtagtttc tgctgctgac ggcccaatgc
    31141 ctcaaactcg tgaacacatc ctattgtctc gtcaagttgg tgttccttac atcgttgtat
    31201 tcttgaacaa agtagacatg gttgatgacg aagaattact agaattagtt gaaatggaag
    31261 ttcgtgacct attaacagaa tacgaattcc ctggtgacga tgttcctgta gttgctggat
    31321 cagctttgaa agctctagaa ggcgacgctt catacgaaga aaaaattctt gaattaatgg
    31381 ctgcagttga cgaatacatc ccaactccag aacgtgacaa cgacaaacca ttcatgatgc
    31441 cagttgaaga cgtgttctca attactggac gtggtactgt tgctacaggt cgtgttgaac
    31501 gtggacaagt tcgcgttggt gacgaagttg aagttgttgg tattgctgaa gaaacttcaa
    31561 aaacaacagt tactggtgtt gaaatgttcc gtaaattgtt agactacgct gaagctggag
    31621 acaacattgg tgctttacta cgtggtgttg cacgtgaaga catccaacgt ggacaagttt
    31681 tagctaaacc aggtacaatc acacctcata caaaattctc tgcagaagta tacgtgttga
    31741 caaaagaaga aggtggacgt catactccat tcttcactaa ctaccgtcca caattctact
    31801 tccgtacaac tgacgtaaca ggtgttgttg aattaccaga aggaactgaa atggttatgc
    31861 ctggcgacaa cgtaacaatg gaagttgaat taatccaccc aatcgctatc gaaaacggta
    31921 ctaaattctc aatccgtgaa ggcggacgta cagttggtgc cggtgtcgtt acagaaatca
    31981 aagcttaatt cacttaagtt ttaaagtaaa gcaacattta ttcggacgta atcgctcgga
    32041 acgaaggtct taggatcttt gttccgagct tttttattta ttgcaggatc aggaaaaaag
    32101 acaggagtat tataaataga agaaacacga aataaaagag tcagtatttt ctaaatgaaa
    32161 ctttatgaaa acaaaatgtt ttttacaaga aaaagccttg ctttgtgagt ttatatatat
    32221 tataatgaca aaggtgcgtt tactttagtg aattgcacag acgtaatcag aatgatttcg
    32281 tcggctaaga aacgagaggt tgcgacacgc ccggacgctt tgccacgaac gagcgtgacg
    32341 gaaattttcg tggagctatg tctacttttc aaaataggcg aaggagggaa caagatggca
    32401 aaacaaaaaa ttcgtatccg tttaaaagcg tatgaacacc gtattttaga tcaatcagcg
    32461 gataaaatcg tggaaactgc aaaaagaact ggagctagcg tgtctggtcc aattccatta
    32521 ccaactgaac gcagtcttta cacaatcatc cgtgcgactc ataaatacaa agattcacgc
    32581 gaacaattcg aaatgcgtac acacaaacgt cttatcgaca ttgtgaaccc aacaccaaaa
    32641 actgttgatg ctttgatgaa gctagactta ccatctggtg taaacatcga aatcaaatta
    32701 taaaactaaa acaaaaataa tggaggtgta ctcatgacca aaggaatctt agggaaaaaa
    32761 gtgggaatga cacaaatctt cactgaatct ggtgaattaa ttccagtaac tgttgtagaa
    32821 gctactccaa acgtagtact acaagtgaaa acaatggaaa cggatggcta cgaagctatt
    32881 caagttggtt accaagatat gcgtgaagtt ttatcaaaca aacctgcgaa aggtcatgtt
    32941 gcaaaagcaa acacggctcc taagcgcttc attcgtgaat tcaaaaatgt tgagctagga
    33001 gaatatgaag taggaaaaga aattacagta gatgttttcc aagcaggaga cattattgat
    33061 gtaacaggta ctacgaaagg taaaggattc caaggggtta tcaaacgcca cggacaaagc
    33121 cgcggaccaa tggctcacgg ttctcgttac catcgtcgtc ctgggtcaat gggtccagtt
    33181 gcacctaacc gtgtatttaa aaacaaacgt cttgctggcc gtatgggtgg caaccgcgta
    33241 actatccaaa acttggaagt tgtacgtgtc gatgcagaaa gaaatgttat cttgatcaaa
    33301 ggtaatgttc ctggagctaa aaaatcttta attactatca aatcagctgt gaaagctaaa
    33361 taattgtagg aagaaaggag gaactaagga atgccgaatg tagcattatt caaacaagat
    33421 ggaagccaaa acggtgaaat cacacttaac gaagaaatct tcggaattga acctaatgaa
    33481 agtgttgttt acgatgcaat cgtaatgcaa cgtgcgtcat taagacaagg aacacacgcg
    33541 gttaaaaacc gtagcgctgt tcgcggcggc ggacgtaaac catggcgtca aaaaggaaca
    33601 ggtcgtgcac gtcaaggatc tatccgctca ccacaatggc gtggaggtgg cgtagtcttt
    33661 ggaccaacac cacgttctta cagctacaaa cttcctaaaa aagttcgtcg tttagcaatg
    33721 aaatctgtat tgtcagaaaa agttgctgaa aacaacttgg tagcaatcga aggcttgaac
    33781 ttcgacgcac caaaaacaaa agaattcaaa caagttcttg ctaacttatc tatcgactct
    33841 aaagtattgg ttgttctaga aaaaggtaac gactttgctg ctttatcagc acgtaactta
    33901 ccaaacgttt ctgtagtaac ttctgataat gtgagtgtgc tagacgttgt atcaaataca
    33961 aaagtgttag caacacaaac tgctcttact caaattgagg aggtgcttgc ataatgaact
    34021 tactagacgt aatcaaacgc ccagtgatca ctgaaaaatc aatgcttgcc atggatgaca
    34081 agaaatacac ttttgaagtg gacactcgcg caaacaaaac tttagtaaaa caagcggtag
    34141 aagctgcttt tgacgttaaa gtcaaaaacg tgaacatcgt gaacgtacgt gcgaaattca
    34201 aacgtatggg caaatatgca ggatatacaa aaaaacgtcg caaagcgatc gttacattga
    34261 ctgaagattc aaaagaaatc caattattcg aagctgctga ataagcacta atagataact
    34321 cattaaatta ggagggaaaa acgtggcgat taaaaagtac aaacctacct caaatggtcg
    34381 tcgtaacatg acaggttctg attttgctga aatcacaaca tcaacacctg aaaaatcatt
    34441 gttacaacca ttgaaaaacc atgctggacg taacaacaac ggccgcatca ctgtacgtca
    34501 tcaaggtggc ggacacaaac gccaatatcg cgtgatcgac ttcaaacgta acaaagataa
    34561 cgttgttgca actgttcaaa cgatcgaata tgatccaaac cgttcagcta acatcgcgtt
    34621 agtacactat gaagatggag tgaaagctta catcttagca cctaaaggac tagaagtggg
    34681 tatgaaactt gtttcaggtc cagaagcaga tatcaaagtc ggaaatgctc ttccattaga
    34741 aaacattcca gttggtactg ttatccacaa cattgaaatg aaacctggta aaggtggaca
    34801 attgatccgt tctgctggta caagtgctca agtacttggt aaagaaggca aatacgtatt
    34861 gatccgcttg aactctggtg aagttcgtat gatcttagct gcatgccgtg ctacaatcgg
    34921 ttctgtaggt aacgaacaac acgaattaat caacatcggt aaagctggac gctctcgttg
    34981 gatgcgtaaa cgcccaactg tacgtggtag cgtaatgaac cctaatgatc acccacacgg
    35041 tggtggtgaa ggtaaagctc ctatcggacg taaagcacca gtttctcctt ggggtcaacc
    35101 agctcttggt tacaaaactc gtaacaaaaa agctaaatca gacaaactta ttgttcgtcg
    35161 tcgcaaaaat aaataattac tttgaccatg tgtcgcacat tttgagagga ggttcaccat
    35221 gggtcgtagt ttaaagaaag gaccttttgt cgatgagcat ttaatgaaaa aagttgaagc
    35281 tcaagcaggg gttgaaaaga aaaaagttat caaaacttgg tcacgccgtt caacagtttt
    35341 cccaagtttt gttggatata ccatcgcagt ttatgatgga cgtaaacatg taccagttta
    35401 catccaagaa gacatggtag gacacaaatt aggtgaattt gcacctacaa gaacttatcg
    35461 tggacatgct gctgacgata agaaaacgaa acgctaattt gaggggagga taaatccaaa
    35521 tggcagaaca aatcacatca gctaaagcaa ctgcaaaaac agttcgcact tcacctcgaa
    35581 aagcccgttt agtaattgac cttatcagag gtaaaagtgt cgcagatgca atttcaatct
    35641 tgaaattcac accaaacaaa tctgctggaa tcattgaaaa agtattgatg tcagcagttg
    35701 cgaatgcaga aaataacttt gacttagatg ttgaaaactt ggtagtaagc gaagcttttg
    35761 ttaacgaagg acctacaatg aaacgtttcc gtcctcgtgc aaaaggatca gcttctccaa
    35821 tcaacaaacg tacaagtcat atcacggtag tcgtatcaga aaaataagga gggataatca
    35881 gtgggtcaaa aagtacatcc aattggaatg cgtgtaggca tcatccgcga ctgggatgct
    35941 aaatggtatg ctgaaaaaga gtatgctgag ttcttacacg aagatttaag aatccgtaaa
    36001 tttatttcaa ctaaacttgc tgatgctgct gtatcaacag ttgaaatcga acgcgcaaca
    36061 ggtcgcgtaa acatttcaat tcacacagct aaaccaggta tggttattgg taaaggcggt
    36121 tctgaagtcg aaagcctaag aaaagaatta aacaaattaa ctggtaaaag aatccatatc
    36181 aatatcgtgg aaatcaaaaa accagatcta gatgcaaaat tagtaggtga aggaatcgca
    36241 cgtcaattag aaaaccgtgt tgctttccgt cgcgctcaaa aacaagccat ccaacgctca
    36301 atgcgtgctg gcgctcaagg gatcaaaact caagtatctg gtcgtctaaa cggagcagat
    36361 atcgctcgtt cagaaggata ctcagaagga actgttcctc ttcatacttt acgtgcggac
    36421 attgattacg catgggaaga agcagataca acatacggaa aactaggagt taaagtgtgg
    36481 atctatcgtg gagaaattct tccaactaaa aaaaacactg agaaaggagg gaaataatca
    36541 tgttagtacc taaacgtgta aaacaccgtc gtgaattccg cggaaaaatg cgcggggaag
    36601 ctaaaggcgg aaaagaagta gcattcggtg aatacggttt gcaagctgtt gattcacatt
    36661 ggatcacaaa ccgccaaatc gaagctgctc gtatcgcaat gactcgttac atgaaacgtg
    36721 gtgggaaagt atggattaaa attttccctc acaaatctta tactgccaaa gcaattgggg
    36781 tacgtatggg ttctggtaaa ggggcacctg aaggatgggt tgcaccagta aaacgtggta
    36841 aaatcatgtt tgaaatcgca ggcgttcctg aagaagtagc tcgtgaagcg ttacgtctag
    36901 cttctcacaa attaccaatg aaaactaaga tcgtaaaacg tgaggaaatg ggtggtgaat
    36961 cgaatgaagg ttaaagaaat cagagaatta accactgccg aaatgcttga tcaagaaaaa
    37021 caattaaaag aagaattgtt taatcttaga ttccaattag caacaggtca attagaaaac
    37081 actgcacgta ttaaagaagt acgtaaatcg attgcacgca tcaaaactgt gttgcgtgaa
    37141 caagctaagt aattatggaa ggaggccatt aagcgtatga ctgaaggaag aaatcaacgc
    37201 aaagtttatc aaggtcgcgt ggtttcagac aaaatggata aaacaattac cgttgctgtc
    37261 gaaacaaaga aaaaccaccc tatctatggc aaacgcatga actattctaa gaaatacaaa
    37321 gtgcatgatg aaaacaacac agcaaaagtt ggcgacatcg tgagaattat ggaaactcgt
    37381 ccattatcag ctacaaaacg tttccgtttg ttggaagtag tcgaagaagc agttattatc
    37441 taataacacg agattttcct atatagattg aatgcaaaat ctgaaaggag gatacacaac
    37501 gtgatccaag cagaaagtcg tttaaaagtc gctgataact caggcgcacg tgaaattttg
    37561 acgatcaaag tccttggtgg atctggtcgt aaaactgcta acatcggtga tgtgatcgtt
    37621 gctacggtta aacaagcaac gccaggtggg gttgtcaaaa aaggtgacgt cgttaaagcc
    37681 gttatcgttc gaacaaaatc aggagctcgt cgtacagacg gttcatatat taaatttgat
    37741 gaaaatgctg cagtgattat tcgtgatgat aaaagccctc gtggaacacg tatctttggg
    37801 ccagttgcac gtgaactacg tgaaaacaac ttcatgaaga tcgtttcact agcaccagaa
    37861 gttttataat cttgagacac ttatcaaagg aggtgcgaaa cagttatgtt tgttaaaaaa
    37921 ggcgataaag ttaaagttat cactggaaaa gacaaaaaca aagaaggcgt tgtactagca
    37981 gcgttcccga aaaaagataa agttattgtc gaaggtgtaa atgtcgttaa aaaacaccaa
    38041 aaaccttctc aagctgctcc tcaaggtgga attgttgaga tggaagcgcc aattcatgtt
    38101 tctaacgtga tggtggttga ctctactggt gtagctggcc gagttggcta caaagaagta
    38161 gacggcaaaa aagtccgtgt ttctaaaaaa accggtgaag ttttagataa ataaaagttt
    38221 aaggaaggag ggtcttactg aatgaaccgc ctaaaagaaa aatatcttaa agaagtaact
    38281 ccatcattga tggaaaaatt taactatagc tctgttatgc aaacacctaa agttgaaaaa
    38341 atcgtcatca acatgggtgt tggtgatgcg gtatcaaacg ctaaaaactt agataaagca
    38401 gttgaagaat taactttgat cactggtcaa aaaccaatga ttacaaaagc taaaaaatca
    38461 atcgctggat tccgtttacg tgaaggaatg ccaattggtg caaaagttac attgcgcgga
    38521 gaaagaatgt acgaattctt agacaaattg gtttctgttt cactaccacg tgtacgtgac
    38581 ttccatggtg taagtaaaaa agcctttgac ggtcgtggaa actatacttt aggtattaaa
    38641 gaacaattga tcttcccaga agtagattat gatttagtag ataaagtacg tggtatggac
    38701 atcgttattg taacaacagc aaactctgat gaagaatctc gtgagttgtt gacacaatta
    38761 ggcatgccat tccaaaaata attaaaggag gcgaactacg tggctaaaaa atcaatgatt
    38821 gctaaaaaca aacgtcctgc aaaattttca acacaagctt atactcgttg cgaacgttgc
    38881 ggacgtccac attcagttta tcgtaagttc catctttgcc gtatttgctt ccgcgaactt
    38941 gcctataaag gtcaaattcc cggcgtgaag aaagctagct ggtaaaaagt aaattcgcat
    39001 aagaaggagg taaaagatca atggtcatga cagatccaat tgcagatttt ctaactcgta
    39061 tccgtaacgc aaacatggtt aaacatgagt ctttagaagt acctgcatca aaaattaaac
    39121 gtgacatcgc tgaaatcttg aaacgtgaag gtttcgttcg tgatgttgaa tacatcgaag
    39181 atgacaaaca aggcgtgatc cgtgttttcc taaaatttgg taaaaacggc gaacgtgtta
    39241 tcacaaactt aaaacgtatc tctaaaccag gtttgcgtgc ttatgtcaaa gctgacgaag
    39301 taccaaaagt attgaacggt ttaggtatcg caatcatttc gacttctgaa ggtgttgtca
    39361 ctgataaaga agctcgtgaa aaaaatatcg gcggcgaagt tatcgcttac gtatggtaat
    39421 caagtagaat ctacaaggag gtgtctttaa gtgagccgta tcggaaataa agtcgttgtt
    39481 atccctgaag gagtaacagt tactcaagac ggaaacaacg ttacagttaa aggaccaaaa
    39541 ggtgaattga ctcgtacttt ttcagcagat atcaaaatga atatcgaagg aaacgaagta
    39601 actttcactc gtccaaacga cagtaaagaa atgaaaacaa ttcatggaac aactcgtgcg
    39661 aacttcaaca acatggttgt aggtgtcagc gaaggtttcc aaaaagcgtt agaactaatc
    39721 ggggttgggt accgtgcaca attacaagga tcaaaacttg tattgaacgt tggttactca
    39781 catccagtag aaatcacacc accagctgga gtaactgttg aagtaccagc taacacacaa
    39841 gtaattgtta aaggtgcgaa caaagaagaa gttggcgaat tagctgcaaa cattcgtgga
    39901 actcgtcctc cagaacctta taaaggtaaa ggtattcgtt atgttggcga atatgtacgc
    39961 cgtaaagaag gtaaaactgg taaataatcg tttagaaaaa ttgcatagaa gccagcagca
    40021 ggaaaaaggc atttggctga agtttacctt caaaaaaata catttttact tgtgccgcag
    40081 cttctatttt tcgaaacgta tttactgctt gaaatagtag tacaataaaa ataaagaggt
    40141 gacaattgtg attacaaaac cagataagaa caaaacacgt caaaagagac atcgtcgtgt
    40201 acgtaacaat atctctggta ctgctgagcg cccacgcttg aacgtttttc gttctaacaa
    40261 aaacatctac gctcaaatta ttgatgacgt agcgggtgta acgctagcaa gtgcctctac
    40321 cttggataaa gaaatttcag gtggaacaaa aacagaagca gcacaagctg tcggtaaact
    40381 agttgctgaa cgtgcagctg aaaaaggcat taaagtagta gtctttgacc gtggtggata
    40441 cctttaccat ggccgtgtgc aagctttagc tgaagctgct cgcgaaaatg gactagaatt
    40501 ttagaagaag gaggaatacc attcatggtt tatatcgatc caaaacattt ggaattagaa
    40561 gaccgcgttg ttgcaattaa ccgtgtaaca aaagttgtta aaggtggacg tcgtctacgt
    40621 tttgctgctt tagttgttgt cggtgacaaa aacggacacg tagggtttgg tacaggtaaa
    40681 gctcaagaag ttcctgaagc aatccgtaaa gcaattgaag atgcgaagaa aaacttagtt
    40741 gaagtaccta tggttggttc aacaatccca cacgaagtaa tcggtgcttt tagtggtggt
    40801 agaatcctaa tgaaacctgc tgtagaaggt tctggggttg ctgctggtgg accagttcgt
    40861 gccgtattag aattagctgg ggtagcagat atcacttcta aatctttagg ctcaaataca
    40921 ccaatcaacg ttgttcgcgc aacagttgaa ggtttgaaac aattgaaacg tgcagaagaa
    40981 gttgcagaac ttcgcggcaa atcagttgaa gaattaatcg gataaggagg acaacaataa
    41041 tggctgaatt aaaagttact ttaaaacgca gtattatcgg acgtcctcaa aaccaacgcg
    41101 atacagttaa agcgttaggt cttacaaaga ttaacagttc agtggttaag ccttctaatg
    41161 aagcaattaa aggcatgatc aatactgttt ctcacctagt ggacgttgaa gaaatttaat
    41221 ttagaaaaat cataactaga acattaagga ggtgccaaac ctatgaaact tcatgaatta
    41281 aaacctgctg aaggttcacg ccaagtacgt aaccgtgttg gtcgtggtac ttcatctggt
    41341 aacggaaaaa ctgccggacg cggacaaaaa ggacaaaaag ctcgttcagg cggcggtgtg
    41401 cgtttaggat ttgaaggggg acaaacacca ttgttccgtc gtttaccaaa acgaggattc
    41461 acaaacgtga accgtaaaga ctatgcagtc attaatcttg atgtattgaa ccgctttgaa
    41521 gacggagctg aagtaactcc tgttgcttta gttgaagctg gaatcgtgaa aaacgaaaaa
    41581 gctggaatca aagtattagc taatggggaa ctaaacaaga aattaactgt gaaagcagca
    41641 aaattctcta aagcagctca agaagcaatt gaagcggctg gtggatcaat cgaggtgatc
    41701 taatgtttag actattaaaa gatgctttta aagtaaaaga cattagatcg aaaatcctat
    41761 ttactgtatt tgtgttgttt gtatttcgcc taggtgcaca tattactgta ccaggcgtaa
    41821 atgctgcttc attgcaaaac ctttcttctt tgccattttt caacatgttg aacttggtaa
    41881 gtggtagtgc gatgcaaaac ttttccattt tctcaatggg ggtttcacca tacattactg
    41941 cttctatcgt cattcagctt cttcaaatgg acatcgtgcc tcgatttgtt gaatggtcga
    42001 aacaagggga agttggtcgt aaaaaactga atcaagcgac tcgatatctg acattagtat
    42061 tggcatttat ccaatctgtc ggtatcactg ctggattcaa tcaatacgct caattaggtt
    42121 tcgtgaataa tcctaacgta gcaacttacg taacgatcgg tttgattcta acagccggta
    42181 cgatgtttgt tacttggcta ggagaacaga tcacagaaaa aggtatcgga aatggcgtat
    42241 caatgatcat ttttgcaggt atcatttcta gattgcctga aagtatcaga gaaatttatg
    42301 aagattactt tataaatatt gatccttcag acatatggaa atctgctatt tttgtggcca
    42361 ttttactagt agcggtcttg ttgatcgtga cattcgtaac atttttccaa caagctgaac
    42421 gcaagatccc gatccaatat acgaaacgcg tagctggcgc gcctacaagc agctatctgc
    42481 cgttgaaagt aaatgcagct ggagttattc cagttatctt tgctagttcg tttattgcta
    42541 cgccaaatgc tgttttacaa gcattgagcg gtagattcgg ttctgaaaac tggtacaaag
    42601 tgatgatgga gatctttaac tataatacag cacctggtgc tacgatttat acgattctga
    42661 tcgttgcctt cactttcttc tacgcctttg ttcaagtaaa ccctgagaaa ttagcggaaa
    42721 acttgcaaaa gcaaggaagt tatataccaa gcgtacgccc tggtaagggg actgaagagt
    42781 atgtctcaag attactgatg cgtttaagta cagttggtgc agtcttcctt ggtttagtgg
    42841 cattgcttcc aatcattgct caaatggttt gggatcttcc acaatcaatc ggattaggcg
    42901 gtacaagctt actgatcgtt atcggtgtag ctttagaaac tgcgaaacag ttagaaggct
    42961 taatgctgaa acgtaaatat gttggattca ttgagtaaga aagcgtgttg aggtttcccc
    43021 tcaacacacg ttctcacaca tttattagga gggaaaacaa tgaacctcat tttaatgggg
    43081 ctgccaggcg caggtaaagg aacacaagct gaaaaaatca ttgatgctta tggtattccg
    43141 cacatctcaa caggagatat gttccgcgca gcgatgcaaa acgaaacagc tcttggcctg
    43201 gaagcaaagt cttatatgga taaaggtgca ttagttcctg atgaagtaac aaatggaatc
    43261 gtgaaagagc gactagctga accagataca gagaaaggct tcttattaga tggctttcca
    43321 cgtacgctag atcaagcaaa agctctggac gcaatgctaa aagatttgaa taaaaaaatt
    43381 gatgctgtca tcgatatcca tgttggtgaa gaaatcttgg tcgaacgtct agccggccga
    43441 tttatctgca gcaattgtgg agcaacttac cataaaattt tcaaccctac aaaagttgaa
    43501 gacacatgtg atcgttgcgg tgggcatgaa ttctatcaaa gagaagatga taaacctgag
    43561 acggttaaaa atcgtctggc tatcaacatc aaaaatagtg aaccaatctt ggcttattat
    43621 aaagaacaag gtttgctaaa cactattgat ggtgctcgtg aaatcgatgc tgtatttgca
    43681 gatgtacaaa aaatcattga aaaatagcac gattgcccgt agccgacttg cattatgcag
    43741 ttactcttgt caagttcaat aaattatgat agaatatgtc agtccgactt ctagagaatc
    43801 ctctagatga tccattttga gaattttgag gtgggaactg cagtttccgc cattttatcg
    43861 tgtgaaaatg cgaaacaagt acaaggaggt actgtgcgtg gcaaaagaag atatgattga
    43921 agtcgaaggt acagtcgtcg aaactttgcc gaatgcaatg tttaaagttg aactagaaaa
    43981 cggacaccaa gttcttgcta ctgtttcagg taaaattcgt atgcattaca tccgtatctt
    44041 acctggtgac aaagtaacgg ttgaattgtc tccatacgat cttaaccgtg gccgtattac
    44101 ttatcgcttt aaataattgt actccgtaaa actacaggag gtattatcat gaaagtaaga
    44161 ccatcagtaa aaccaatgtg cgaacattgt aaagtaattc gtcgtaaagg acgcgttatg
    44221 gtgatttgcc cagcaaatcc aaaacataaa caacgtcaag gataattgga ggtgctataa
    44281 atatggctcg tattgcagga gtagatattc ctcgtgataa acgtgtagta gtttcactta
    44341 cttatatcta tggtattggt aacactactg ctaaaaagat cttagctgat gctggtgtat
    44401 ctgaagatgt tcgcgttcgt gaattaacga atgaacaaac agatgctatc cgtgcagaag
    44461 tagataaatt aaaagttgaa ggggatcttc gtcgtgaagt gaacttgaac atcaaacgtt
    44521 tgatggaaat cggttcttac cgtggaatcc gtcatcgccg tggattgcct gttcgtggac
    44581 aaaacacgaa aaacaatgca cgtactcgta aaggcccagc tcgcgctatc gctggcaaga
    44641 aaaaataatt cttaagtgaa ggaggttaaa acttcatggc agcaaaaaaa gtgaatcgta
    44701 aacgccgtgt gaagaagaat atagaagcag gtattgcaca tatccattct acattcaata
    44761 acactatcat catgatcact gatactcacg gaaatgcatt agcgtggtca tcagctggtt
    44821 cattaggttt caaaggaagc aaaaaatcaa ctccttttgc cgctcaaatg gcagcagaaa
    44881 ctgctacaaa agcagcaatg gaacacggat taaagactgt tgaagtaaca gttaaaggac
    44941 ctggttctgg acgtgaagca gcaattcgtt cattacaagc tacaggttta gaagtgactg
    45001 caattcgtga cgtgactcca gttcctcata atggtgcccg ccctccaaaa cgccgtcgtg
    45061 tttaatgagt gccgctcatt ttgagtttta tcagtaacgt cattgaacaa gacacttttc
    45121 gttttgaaag gggtaaagat aagaatgatt gagttcgaaa aaccaagaat cgcaaaaatt
    45181 gatgaagaaa aagattatgg caagttcatc gttgaacctc ttgaaagagg gtatgggact
    45241 actttaggga attccctacg tcgtatttta ttatcttctt tgccaggtgc tgccatcact
    45301 aatattcaaa tcgatggtgt actgcatgaa ttctctacca tcaaaggtgt tagagaagac
    45361 gtcactcaaa tcatcttgaa cattaaaggc ttagcgctga aaatgtatgg ccaagaagaa
    45421 aaaaaccttg aaatcgatat taccggacct gctacagtaa ctgctggcga tattatcgtt
    45481 gatagtgatg tagaaattct taacaaagat atgtacatct gtagtgtctc tgaaggagct
    45541 acgttccatg ctcgcttaac agtgaaacct ggacgtggtt atgttcaagc agatgaaaat
    45601 aaaaaagaag atatgccaat tggcgttctt ccagtcgatt caatctacac acctgttcgt
    45661 cgtgtgaact atcaagttga gaacacacgt gttggacacc gtgaagattt tgataaattg
    45721 acgatggaga tttggacaga tggttcaatt gaaccgttgg aagcaatgag tttgtctgcg
    45781 aaaatcatga ctgaacattt ggatattttt gtgaacttaa ctgatgaagc aaaaaatgct
    45841 gaaatcatgg tcgaaaaaga agaaacacaa aaagaaaaaa tgttggaaat gacgatcgaa
    45901 gagttagact tgtctgttcg ctcttacaat tgtttgaaac gtgcaggaat caatactgta
    45961 caagaactta caaataaatc tgagcctgaa atgatcaaag tccgtaactt aggacgtaaa
    46021 tcattagaag aagttaaagc aaaattgcac gacttaggtt taggtttacg taaagaagat
    46081 taaaaccttt tacgaaggag ggaaaccaac gtgagttatc gtaaactagg acgcacatct
    46141 agccaacgta aagcgatgtt gcgtgattta acaactgatt tattaatcaa cgaacgtatt
    46201 gtgactactg aagctcgtgc gaaagaagtt cgctcaactg cagaaaaaat gatcacttta
    46261 ggtaaacgcg gagatttgca tgcacgtcgt caagctgcgg ctttcgtacg taatgaagtt
    46321 gccagcgtgc gtgaagaaaa tgaggacatc gtcattgaat cagctttaca aaaactgttc
    46381 aacgatattg cacctcgtta cgcagaacgt caaggcggat acacacgcat tttgaagaca
    46441 gaaccaagac gcggtgatgg tgcaccaatg gttgtcttag aacttgtcta aaatcacact
    46501 aataacatca ctttatagat gattctttta gagtgttatg atgttggaag atatttttcc
    46561 gagtctagct cagcgctgct cctcaagcga atatcgcttg gggagtaagc actcatcata
    46621 aagtgttttt ttataccttc aattacctgt catgacagat atgtttgaag gcataaaaaa
    46681 acattttaag cgagaaataa ttgaaaattt tgtctttttt tgatagtgtt agtaggaact
    46741 ttaggatgaa gaaggagtaa tcatggagcc aattattgaa ttagaaaaga taaattataa
    46801 ataccagcca gatgatcttc gccccgcatt gaaagatgtc tcctttacaa ttgataaagg
    46861 tgagtggatc gctattattg gacacaatgg ttcaggaaaa tcgacacttg ccaaaacgat
    46921 caatggattg cttctgccag aatcagggat cgtgaaagtg ggaaatcaaa tattggatga
    46981 agaaaatatt tggacgatcc gacagatggt tggaatggtt tttcaaaatc cagacaatca
    47041 gtttgttggt tcgacagtag aagatgacgt tgcttttggt cttgaaaatc aagggattcc
    47101 cagagaagaa atgctagtgc gtgtgaaaga tgcacttgag aaagttagaa tggctgagtt
    47161 tgcttcacgc gagcctgctc gtttatcagg agggcaaaaa caacgtgttg ccattgccgg
    47221 cgtggttgcc ttacgacctg atatcattat tttagatgaa gcaaccagta tgctagatcc
    47281 agaaggtcgt gaagaagtga tctctacgat caagaagatc aaagaagaaa gtcaattgac
    47341 cgttatatct atcactcatg atattgatga agcagctaat gccaatcgga tactagtgat
    47401 gagacaaggc gaacttgtcc gtgaaggtac accaaaagaa attttttctg ctggtcctga
    47461 actgatcgac ttaggtctag atctaccatt tcctgaaaaa ctgaagagcg cactgaaaga
    47521 acgtggggtt gatgtgccca gtgaatatat gacagaagaa aggatggtgg attggttatg
    47581 gacatccgtt ttgaacaagt agacttcact tatcagccga atacaccatt tgaacaaaga
    47641 gccttatttg acatcaatat gacgatcaaa gaaaacagtt atactgcatt agtagggcat
    47701 accggaagcg ggaaatcaac cttacttcag catttgaatg cgcttgttaa gccaacaagc
    47761 ggtacagtcc atattggaga acgagatatt cagccggata cggataataa gaatttaaag
    47821 cccatccgaa aaaaagttgg catcgtcttt cagtttccag aagcacagct attcgaagaa
    47881 acggtagcaa aagacattgc tttcggtcct aaaaactttg gtgtcagcga agaagaagca
    47941 ctagtcctag caaaagaaac attagaacaa gttgggctgg atgaaagcta tttggaacgt
    48001 tcgccatttg aactttcagg agggcaaatg cgtagagtag caatcgctgg tgtgcttgcc
    48061 atgaggccgg aagtacttgt attggatgaa ccaacggcag gacttgatcc ccagggtaga
    48121 aaagaaatga tggagatgtt ttggcgtctt cataaggaac agcagatcac tattgtatta
    48181 gttacgcatt taatggatga tgtagccaat tttgcagatt atgtctatgt gttggaaaag
    48241 ggacgaatcg ttaatagcgg ggagccgcaa gaggtctttc aaaacattga atggctaaaa
    48301 gaaaaacaac taggcgtacc tactgccacc gagtttgcag aagaattaat ggctaaaggg
    48361 atgaactttg ctaccttgcc tttgacagct gaagaattag cagacgctat cgtccattat
    48421 gctggaggaa atgcccatga tgaataaatt gatatttggt cgttatatgc ctggagattc
    48481 cttgatccat cgattggatc cccgagctaa attattagcc agtttttatt ttatcggaat
    48541 cattttttta gcaaataatt gggaaacgta ccttttgttg gcatttttca cattactttc
    48601 tgtcttcttg tcaaaagtag atttgaaatt ttttatccga ggcattcgcc cattgatctg
    48661 gctaatcctc ttcactgtag ctttgcaggt cttgtttaca agtggcggag aaacttattg
    48721 gagttggggt gttttccata tcactgaatt tggtgtgatg aacggcctct ttattttctg
    48781 tcgattcgtg ttgatcattt ttatgtcgac tctactcacg cttactacac cgcctttgga
    48841 attatcggat gcgattgagt acttgcttcg tccattgaaa gccgtacggt tcccagtcca
    48901 cgaaatttca ttgatgctgt cgattgctct tcgttttgtg cctacattga tggacgaaac
    48961 agaaaagatc atgaacgctc agcgcgctcg cggtgttgat tttggtgaag gaaacctagt
    49021 tcagaaaatg aaagcagtca ttcctttgct gattccttta tttgtcagca gcttcaaccg
    49081 tgcggaagat cttgcgactg ctatggaagc aagaggctac aaaggtgggg aaggaagaac
    49141 gaaatatcgt gtattgcatt ggcataatag agacacgatg gttatcattg cttttgtatt
    49201 attaactgtg ctactgatct ttttgagagg ataaacgttc agtgggccgc tatctattct
    49261 tttcttgctg atctgtttgc aagaaaggat gcgagcagtg tatgacaaga aatattaggg
    49321 tctgtggcag actaaggtca caggcctctt tcttttatct gaaaagaaag aacgaaaaaa
    49381 ttttataaaa aaatcattat tccagataac cttttaggaa caaaggagag aaaaacatgg
    49441 tacgttacaa agcaatcatt gcatacgacg gaacaaattt taatggattc caaaaacagc
    49501 caaatgggcg tactgttcaa gaagaagtag aaaaaacatt acaaaaaatg gcaaatggaa
    49561 aagaaatcac ggtctttggt tcgggaagga cagatgccgg agtacatgcg attgggcaag
    49621 taatccattt tgattatcca gaggagcgtc cgttagaacg aatgcgtttc gcgctagata
    49681 cccagtcacc agaggatatt gctgtaagac aagtagaaat tgtttcagaa gacttccacg
    49741 caagatattt ggtcaaagaa aagacgtatc aatttcgagt ggatataggg aaaccacgca
    49801 gtccttttcg acgccattat gcaagttatt ttccttaccc tcttgatttg gaaaaaattc
    49861 agcgggcgct tcctgattta ttagggactc acgatttcac ctctttttgt gcatcaggaa
    49921 gctcgataga ggataaagta cgtacgatct acgaggctaa gatggaggta aatgaaacaa
    49981 aagacgaatt gttgtttaca tttaggggaa atggcttctt gtataagatg atacggatct
    50041 tagtaggaac gcttttgaaa attggcaatg gccgattgcc tgaagatagt atcccagata
    50101 ttattgcgaa aaaagatcgt aatgcagcag gacctacagc acatcctgaa ggactttatt
    50161 tgtatgaagt gaaatatgaa taagaggttg tgacagaagt ggtcagcttc aagaaataag
    50221 aaggaattta cgaaaattac ttctcaaaat tttgcaaaat ttcggcttat tttcgaagga
    50281 gctacttctg gaacaccgtg aatcagaggt tgtgaaaaag ctgccctgca tcaagtaata
    50341 agaacaacca aacaaaaata gctcgtcata tttttcgttt ggttgggctt attaccgcag
    50401 atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa atcagcgttt tgacctgagt
    50461 attagagaaa caaacggaaa aatagttcct catattttgc cgtttgttga gctaataccg
    50521 aaggtcagct gattgaacac cgtttaagaa agaggtcgtg agcctgtcat ttaactctga
    50581 gtcacaagta acaatttcaa aaaacagctt ctcatctttg atgaatatcg tgacttgtgg
    50641 acgaggcgtt gataagtgaa tcatcttttt gttacctatt ttttctgtaa ccaaaaaacg
    50701 atggaacgtt gttttgattg cccttaatga ggacagtcgg aacaggtttg tcatttttat
    50761 atttaaatgg cacaaatttt tctaattatt tgtcatttag cgctgaaaat ttttgctatt
    50821 tatctaaaaa agtgataact acttaagatg aagcagaata tttacgccat acggcataca
    50881 accgcgaaaa atataaaaaa aagaaaacga ttgaacgcgt ttttgcagtc gcaaaggaaa
    50941 agcatggaat gcgctacatc aagtatcgag acttggaaaa caatcaggtg tatacgctga
    51001 ttgtttttgc tgcaatgaat ctgaaaaagt tgtccacttg gcagttggaa catatgtctt
    51061 catatctttt attatacaaa aatagcaaaa gcaccagtct acactcttag taaagaggaa
    51121 actgatcctt ttgtctacat tcttaaagta cttaaactaa gtactttatt ttactcttta
    51181 ggattgatac agtagcatct atccctttat taataatctt ttattttttt aaataaagat
    51241 gttgtctcca tcttcaggca ctaaaacttt ttcggaaatt cctttttctt tattgaaaga
    51301 ctttaattct tctcttgaca gtccccaatg gtttacagct tccatatgaa cggcaataat
    51361 tgtggaatct ggtattgctt tatgcaattc atataaatcg tctttcccca taataagaga
    51421 ttctccaact aaaaaatgat tatctccagc atttgctact atgatttctg gattatactg
    51481 atcaatagtt tgtttaacag cttcatacca aactgtatct cctgtaaagt atagtgtctt
    51541 ctcagtcgaa tgtttgaaaa caataccgca aacattacct acataattca agatttctcc
    51601 tctgccatgt ttgccttttg ttttaaccaa ttgtatagat tcaaaaactg tgtcttctcg
    51661 tagtacctcc acatttttaa aaccatatag tcgaatttca ttcgcatcat tttcatcttg
    51721 cacaaataat tttatttctt taggtaggac ctctttcgct atatcgtccc aatgatctag
    51781 atgtaaatgt gttacaataa cagcatcaat accgttgata atatcttcga ctggcattgg
    51841 caaatcaact agaggattca tttgatcttt tcttggtgat ggaaaaggac ctttcgaaat
    51901 aaatggtgga agtgttcctt tttccgctaa cattggatca attaaaaatt ttttacctcc
    51961 atagtctaag atagctgttg cattacgaat gtgatgtata ttcataaacg ttgtctcctt
    52021 tttgttgata tgaaatattt ttagtataat tcaattccat acaataaata aaccgaacaa
    52081 aaaaagtctt ttactatttt tacttaattt atgaaaggtt gtttaccatg ttagaccaaa
    52141 cagatataaa tatcattgag gaactatcaa aaaatagtcg aatcacaatg aaagaacttg
    52201 gagaaaaagt acatttgact ggacccgctg tctcggcgag agtgacaaaa ctagaggaaa
    52261 gtggagttat tgaaaattat acagtaaaat taaatatgga aaagctaggg ttttcgattc
    52321 acgctttttt aattataatt acgcaaaatt t
//
LOCUS       DAZBSA010000006        50010 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000006 DAZBSA010000000
VERSION     DAZBSA010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.006"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..801)
                     /locus_tag="ACHGMT_000337"
     CDS             complement(<1..801)
                     /locus_tag="ACHGMT_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFN6834114.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILV"
     gene            complement(774..1454)
                     /locus_tag="ACHGMT_000338"
     CDS             complement(774..1454)
                     /locus_tag="ACHGMT_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFN6834115.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1477..2415)
                     /locus_tag="ACHGMT_000339"
     CDS             complement(1477..2415)
                     /locus_tag="ACHGMT_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFN6834116.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2660..3511)
                     /gene="ispE"
                     /locus_tag="ACHGMT_000340"
     CDS             complement(2660..3511)
                     /gene="ispE"
                     /locus_tag="ACHGMT_000340"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFN6834117.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(3665..6238)
                     /gene="mprF"
                     /locus_tag="ACHGMT_000341"
     CDS             complement(3665..6238)
                     /gene="mprF"
                     /locus_tag="ACHGMT_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFN6834118.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6379..7185
                     /gene="proC"
                     /locus_tag="ACHGMT_000342"
     CDS             6379..7185
                     /gene="proC"
                     /locus_tag="ACHGMT_000342"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFN6834119.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7264..8820)
                     /gene="rny"
                     /locus_tag="ACHGMT_000343"
     CDS             complement(7264..8820)
                     /gene="rny"
                     /locus_tag="ACHGMT_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFN6834120.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9231..10280)
                     /gene="recA"
                     /locus_tag="ACHGMT_000344"
     CDS             complement(9231..10280)
                     /gene="recA"
                     /locus_tag="ACHGMT_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFN6834121.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10389..11630)
                     /locus_tag="ACHGMT_000345"
     CDS             complement(10389..11630)
                     /locus_tag="ACHGMT_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFN6834122.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(11870..12448)
                     /gene="pgsA"
                     /locus_tag="ACHGMT_000346"
     CDS             complement(11870..12448)
                     /gene="pgsA"
                     /locus_tag="ACHGMT_000346"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFN6834123.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(12657..13580)
                     /locus_tag="ACHGMT_000347"
     CDS             complement(12657..13580)
                     /locus_tag="ACHGMT_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFN6834124.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(14319..15605)
                     /locus_tag="ACHGMT_000348"
     CDS             complement(14319..15605)
                     /locus_tag="ACHGMT_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFN6834125.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(15598..16866)
                     /locus_tag="ACHGMT_000349"
     CDS             complement(15598..16866)
                     /locus_tag="ACHGMT_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFN6834126.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            17118..17579
                     /gene="mscL"
                     /locus_tag="ACHGMT_000350"
     CDS             17118..17579
                     /gene="mscL"
                     /locus_tag="ACHGMT_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFN6834127.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(17638..18084)
                     /locus_tag="ACHGMT_000351"
     CDS             complement(17638..18084)
                     /locus_tag="ACHGMT_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6834128.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(18177..18422)
                     /locus_tag="ACHGMT_000352"
     CDS             complement(18177..18422)
                     /locus_tag="ACHGMT_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFN6834129.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(18537..19115)
                     /locus_tag="ACHGMT_000353"
     CDS             complement(18537..19115)
                     /locus_tag="ACHGMT_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFN6834130.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(19192..19722)
                     /locus_tag="ACHGMT_000354"
     CDS             complement(19192..19722)
                     /locus_tag="ACHGMT_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFN6834131.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(19726..20586)
                     /gene="rlmB"
                     /locus_tag="ACHGMT_000355"
     CDS             complement(19726..20586)
                     /gene="rlmB"
                     /locus_tag="ACHGMT_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFN6834132.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(20579..20974)
                     /locus_tag="ACHGMT_000356"
     CDS             complement(20579..20974)
                     /locus_tag="ACHGMT_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFN6834133.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(20971..22383)
                     /gene="cysS"
                     /locus_tag="ACHGMT_000357"
     CDS             complement(20971..22383)
                     /gene="cysS"
                     /locus_tag="ACHGMT_000357"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFN6834134.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(22380..22916)
                     /gene="epsC"
                     /locus_tag="ACHGMT_000358"
     CDS             complement(22380..22916)
                     /gene="epsC"
                     /locus_tag="ACHGMT_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFN6834135.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(23002..23241)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23238..24698)
                     /gene="gltX"
                     /locus_tag="ACHGMT_000359"
     CDS             complement(23238..24698)
                     /gene="gltX"
                     /locus_tag="ACHGMT_000359"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFN6834136.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(24868..26025)
                     /locus_tag="ACHGMT_000360"
     CDS             complement(24868..26025)
                     /locus_tag="ACHGMT_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFN6834137.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(26059..27429)
                     /gene="radA"
                     /locus_tag="ACHGMT_000361"
     CDS             complement(26059..27429)
                     /gene="radA"
                     /locus_tag="ACHGMT_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFN6834138.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(27492..28019)
                     /locus_tag="ACHGMT_000362"
     CDS             complement(27492..28019)
                     /locus_tag="ACHGMT_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFN6834139.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            28249..29055
                     /gene="proB"
                     /locus_tag="ACHGMT_000363"
     CDS             28249..29055
                     /gene="proB"
                     /locus_tag="ACHGMT_000363"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFN6834140.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            29052..30299
                     /locus_tag="ACHGMT_000364"
     CDS             29052..30299
                     /locus_tag="ACHGMT_000364"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFN6834141.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            30375..30719
                     /locus_tag="ACHGMT_000365"
     CDS             30375..30719
                     /locus_tag="ACHGMT_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834142.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(30836..31162)
                     /locus_tag="ACHGMT_000366"
     CDS             complement(30836..31162)
                     /locus_tag="ACHGMT_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834143.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(31777..32979)
                     /locus_tag="ACHGMT_000367"
     CDS             complement(31777..32979)
                     /locus_tag="ACHGMT_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN6834144.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            33096..33611
                     /locus_tag="ACHGMT_000368"
     CDS             33096..33611
                     /locus_tag="ACHGMT_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFN6834145.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(33810..34256)
                     /locus_tag="ACHGMT_000369"
     CDS             complement(33810..34256)
                     /locus_tag="ACHGMT_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFN6834146.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(34303..35781)
                     /gene="msr(C)"
                     /locus_tag="ACHGMT_000370"
     CDS             complement(34303..35781)
                     /gene="msr(C)"
                     /locus_tag="ACHGMT_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFN6834147.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(36135..36764)
                     /locus_tag="ACHGMT_000371"
     CDS             complement(36135..36764)
                     /locus_tag="ACHGMT_000371"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFN6834148.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(36748..37212)
                     /locus_tag="ACHGMT_000372"
     CDS             complement(36748..37212)
                     /locus_tag="ACHGMT_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFN6834149.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(37699..38967)
                     /locus_tag="ACHGMT_000373"
     CDS             complement(37699..38967)
                     /locus_tag="ACHGMT_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFN6834150.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(39198..39935)
                     /locus_tag="ACHGMT_000374"
     CDS             complement(39198..39935)
                     /locus_tag="ACHGMT_000374"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN6834151.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(40002..40571)
                     /locus_tag="ACHGMT_000375"
     CDS             complement(40002..40571)
                     /locus_tag="ACHGMT_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFN6834152.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            40699..41571
                     /locus_tag="ACHGMT_000376"
     CDS             40699..41571
                     /locus_tag="ACHGMT_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN6834153.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            41913..42785
                     /locus_tag="ACHGMT_000377"
     CDS             41913..42785
                     /locus_tag="ACHGMT_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFN6834154.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            42782..44740
                     /gene="nagE"
                     /locus_tag="ACHGMT_000378"
     CDS             42782..44740
                     /gene="nagE"
                     /locus_tag="ACHGMT_000378"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFN6834155.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(45072..45923)
                     /locus_tag="ACHGMT_000379"
     CDS             complement(45072..45923)
                     /locus_tag="ACHGMT_000379"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN6834156.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            46046..46513
                     /locus_tag="ACHGMT_000380"
     CDS             46046..46513
                     /locus_tag="ACHGMT_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFN6834157.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(46776..47258)
                     /locus_tag="ACHGMT_000381"
     CDS             complement(46776..47258)
                     /locus_tag="ACHGMT_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFN6834158.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(47358..48221)
                     /locus_tag="ACHGMT_000382"
     CDS             complement(47358..48221)
                     /locus_tag="ACHGMT_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFN6834159.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            48819..49694
                     /locus_tag="ACHGMT_000383"
     CDS             48819..49694
                     /locus_tag="ACHGMT_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFN6834160.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(49839..49991)
                     /locus_tag="ACHGMT_000384"
                     /pseudo
     CDS             complement(49839..49991)
                     /locus_tag="ACHGMT_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
BASE COUNT        14388 a        10675 c         8915 g        16032 t
ORIGIN      
        1 aaccaatatt cgtttgatga tatttacaat caggaacaaa gcaataaaga ctaatgtgat
       61 tgttgctcct ggaggtgtat ctagataaaa cgagctagtt agtcctgaca gcatccctag
      121 aaatcctaca caaacactga tagcaatcac gacattgaag cttttgccta atcgcatacc
      181 gattgctgcg ggaagaacca tgattgcgga tatcaaaagt gcaccggcaa tcgggatcat
      241 cacagcaata gccacaccag taatcacatt gaacatcatc gacatcaaat gaacgggtag
      301 cccatccacg tgggcggtat cttcatcaaa agtcagaaca tacatcggcc gtttgaataa
      361 aaagaacaat ataccgatgg caacgaataa gattcctaaa aagatgactt gctcccatgt
      421 aatggtcacg atcgaaccaa acagatagga ttggatactt gttgctgaat tccctccgct
      481 caaattcatc aatactaacg ctagcgccaa accaccagcc attaaaatgg caatggagat
      541 ttctgaatag gttcggtaga tcgttcgcag atactccaat atcacagctg ctaatacaac
      601 gactagcatg gtcgtaagtg taggattaag gttcaaaaag aatcctaacg ctacccctgc
      661 taaagagaca tgagaaagcg tatcagccat cagcgattgt cttctgatca ctaaaaacac
      721 acctagcatc ggggcgattc cggcaatgaa gatagcggca agaaacgctc ttctcatgaa
      781 ttcatatgaa agcaacgcca ttgagaatcc tcctttctca ccagtcggat ctgtctgtcc
      841 gcatactgtt tgatatcttc atgatcatgg gtgatcatta gaactgattt cccatggaca
      901 tgcgcactat gtcggagaag acgataaaat tcatttctgg attcttcatc catacctgtt
      961 gttggttcat caagaacaaa taagtcaggg tcagtagcaa aaatacgcgc taaactgatt
     1021 cgctgctttt gtccgccaga caattcacct attcgccgat ttcgcatatc ccacatccct
     1081 acagcttcta aagcacgctg tacatgttga tgatcttttt cagagagacg cttgaaccag
     1141 cggcctcttg gatatcttcc tgaacggact aattcgatca ccgtactagg aaaacctgca
     1201 ttgaaagatg caacttgttg aggaatataa ccgatactta atttttttcc ttcttgattt
     1261 ttctcagcaa tctttatcat tccttttgaa ggtttcagta atcctaacga tgctttcacc
     1321 aaggttgact tggctgctcc attttctcca gttaaaatga caaattcacc cgcatctaca
     1381 tgataagaga catcttctag tacaggttca tcatcatagt aaaaggtcaa atcagatatt
     1441 tcgatataac gcaccttaca acctccattc taaaacttaa ttgatacttt cttttaaagc
     1501 tttcagattt ttttccatga cagaaatata attttcgcct gctttgattt gttcattagt
     1561 caagctttct aaaggattca acacttctag cttcactcct gtttcattcg acaatgtctc
     1621 agctaccttc gaagaggcat tttcttcaaa ataaatcact ttgatgttgt ttttcttcac
     1681 aaattctttt agctcagcta agcgacttgg tgtgggttcc tgatctggtg aaacgcccga
     1741 aatcgctact tgttttaagt cgtattgatt cgctaaataa gcaaaagcag catgctgagt
     1801 gacaaatgtg cggtttgttg catcttttag ctcatcggag tatttttgat ccagtgcttc
     1861 aagctttttg atataagcat cggcattctt cgtaaaagtt tcctttttat ctggatattt
     1921 ttcgcctaat tcttcagcaa tcgttctgac ttcttttata gctaataccg gatcgagcca
     1981 aacatgtgga tcgtattcat gtgaatgacc atgcccttct tctgattctt catgttcgtc
     2041 tgcttcttgc gattctaaac ggtcgattcc tttagctgct tcgatcatca aggtctgttt
     2101 gctatccact gaatccttta acgaatccac aaaaaattcc atgtctgaac tattatacac
     2161 gaaaacatct gcatcggaaa tttctgctct ttcttttgca cttggttcga aatcatgcgg
     2221 ctccgttcca gcagggatca aaagtttcac gtttccttca tcccctacga tctcttttgt
     2281 aaaatcatac atcggataaa aagtcgtaac gatcgtcaaa tcctcttttt tgtctgcttc
     2341 cttgttcgta tttccacatg ctgcaaacag taaagcacct atcattaccg tagctgtcaa
     2401 aagatatttt ttcatttata tgctcctttc aatgaacaaa tcggaatttt acgatttatt
     2461 aacgtgaact aatgtatcaa aataaaaaaa cacagtcaat agaaatccat caatttctga
     2521 tttaagcatg tatattcttg attaaatcgt aaaatggatg agttgctgct catatctagc
     2581 catttcacta caataaaaaa ctgaagaata ccattttagg attcctcagt ttttcatcac
     2641 ctattttact tcttatattt tattgcaggg ttcgtactaa ataaacttcc tcacaaaaac
     2701 cttttaaccc attatatact ctctgcgctc ttgaataatg ttgacacaag gcaaaaacgg
     2761 ttggtccgct tccactcatc aatgcagcat ctgctccgta cttgatcata cgctctttga
     2821 tttgatggat gactggatgc cgctttcccg ttatatcttc taaactgttt cccatttttt
     2881 tgatcatctg ctgataatct tgctctcgga tggcttcagc aagtccgtgg atgtcttgat
     2941 gttggatggc ttgcgtatct acttctctaa acactgtacg tgtagagaca ctcattcttg
     3001 gtttgaccaa cacaacccag cactgtggca tagaaggtaa tacttggatt tcttcccctt
     3061 tgccaccgac aaatgccgta cttccgtaga cacagtaagg aacatctgta ccgacttcca
     3121 aaccgatttc tgccagttct tctaacgaac agcccagatt ccataattgg ttgatccctc
     3181 ttagagcagc tgcacagtct gtacttcctc cgcctaatcc agctgctact ggaatattct
     3241 tcttaacaaa gatcttgaca ccggttttga tattgtattt attttttagc aataaagcag
     3301 cttgataaac attgttccgt tcatctaccg gtaaaaatgc tttatttgtt tctacaatga
     3361 ttttgtcttc tgttatttct tcaaaaaata gatgatctgc caagtctacg ctcgacatca
     3421 ccatttccaa ctcatgatac ccatcttgcc gtctatataa tacatccagt cctaaattta
     3481 tcttggctgg cgctttttca atcaattcca tcctctcacc cattcgaaaa gtctaagtcg
     3541 caagtattct atcataggga aaaataaaag tatagcttat gtctgcctag tggttgattt
     3601 tgtcatcgtt tcaacagctt tcacagaatt ttctcttcat aatccaaaca agttctagtg
     3661 attcttataa ttctgtgagc acttcttctg ccaaactcgg aacatttcct ttttcttttc
     3721 ctatcagact gagtagctgc atggtagcaa atccgaaatt acttcctttc ctataagcaa
     3781 aataacgtcc ttcccaatga tcgacatatt ttaatttctt ttcataagca gactggaagg
     3841 aatagaaaaa attaccataa ttatatacga tatgcatgac tttttcttga aagaaagaaa
     3901 aatctgtttc tccaacatta gctaatggag ctaagcctag attggcttcc gagtagccca
     3961 ttttctcgta tacaataaac agattggcta acagatagtc agctaattcc tcagattctt
     4021 taggtaagac ccgcaacaaa tcatatcccg cctgcttctt cgtcatcggc tgagcggtga
     4081 tgaagccaac gactttttga tctttacgga caataccgac accgctagat aatacatatg
     4141 atttatcaaa tcgtcccaca gagaagtatt tttctcgtgc accttgaagc catttttgtg
     4201 aaacttcatt tacagcttga tgtaattctt ccggcagttg atcgtaccaa gtaaaatggt
     4261 atccttcttt catcaaacgt tgatattcaa gttgctgact cagccgagac aagtcttctg
     4321 ttgctagatc aacaatccct gcttcaccta ctttcgtgaa gtgaaaaccc aattcatgaa
     4381 gcatcactac gtagctttct gatatcttat aaaacgcaag ctgatagcct aaacaatctg
     4441 cttgtttcat aaactgattc gttgctgccc gcatcatttt aggattgcca actggatctc
     4501 ctaaaacaaa gcatttgttt cctttgatct gataaccaaa aacgacttga tcttggtcgt
     4561 cttcttggta ataaaaataa ctgtaacctg acaagcgaag ctgatggctt gtagctgtgc
     4621 ctccgtatgt ttccaataaa cggtcaaaac ggtctttttc gtactcactg ccaagcttcg
     4681 tagtatcttc agctaaatat tgataaagcg ccactaaagc tagcaatgag atgcccaaac
     4741 caataagccc tgagaaccac acatcatctg aaggaaacaa aataaaatga tttcctgtta
     4801 ttcgaccgac taaaaagcgt ccgctgtgat agcctgcaat tgcgtaaacg ataaacaaaa
     4861 agccgaacaa gctagcatca aagattatag ctccccaaga ataaacgaat tttgttcgat
     4921 aaaattcttt tctggataac cacacagcta acagaatcaa gacatatacc aatatcaact
     4981 gccaagataa cgtacgtgtg attgtattta aaataccaaa agatagtaaa gcaatcgtag
     5041 gcagaaatgc ctttttgact tttactgaaa gtgctctagc aaggccgagt aataaaaagc
     5101 caaccaatag attcactgtc tgatctaaaa aattgaatga gaacggcaat aatacttgaa
     5161 atagccgact gattgcagac agattagtga ttgttgatag aagtaccatc atgatccccg
     5221 caaaatagac agcacttacg agcacgatat gcgcaacctt ttggaaaaac agtcgcggaa
     5281 tgttatctaa aaatcgattg attttgactc ctgtatgtgt gatgaacaat agaagtcccg
     5341 tcataaaagg tagaacataa taaaacaacc gataatataa tagccaaact actgctacat
     5401 cttgccgaac agacaattga ctcattccta agatcatcaa aacgtcaaaa gtccccattc
     5461 cacctgggac catcgtcagc attcctatca atgtagcaat catgaacata ggatagatcg
     5521 aaagcatcga aatatctatt ctcatcaact tgccgatgat caaaaatacc gcaagggctc
     5581 ccgtccattg tccaaaggat gccagtagca gaccaagcaa gccttttgga taaaactcga
     5641 agaacaatgt ctttctcttt agataagcaa aaaagaaaat gactggtgct aaaaaactac
     5701 cgccgaaaag ccagatccaa tagcttcgaa aaatactttc tgactgaata aaaaagacat
     5761 caataaatgt aagaaacgac catacagaca aaccagtcag cataaatagt gcgacttttg
     5821 atacagtggc taaaaccatt tttctatttg tctgctttcc ataaaaacta gcccgcagac
     5881 ttgctcctac cacaccacca aaaccggcga gattgttgat cgtgtttgtt acccacgcag
     5941 caagaagaaa ctcttttctt tccatttttg gttttccttg tttttctaag actctgatcg
     6001 tcacccaatc atacaacaac atcggagtga cacccagcag tccagccaga atcatgagca
     6061 ggatcgtcat gcggctttgt tgtcccatcc tttgccaaat atcttgccaa ctcatgccat
     6121 tagcaatatt tgcgacttga tttgccacaa agactaacac ggagccgaaa aaaatcagtt
     6181 ttaaaatcag actatgttcc tttatccatt gcagcatcaa gtatcgttta tttttcacat
     6241 tttcccattc ctaacttgta tacttttgtg tcatacccca atgttatctt atttatttta
     6301 atttttccac tttagacctg ttctttgctt gcgatgatag aaaaagaaag atatcctaat
     6361 gctaaaggag aggataacat gaaagtagga tttatcggca cagggaatat ggcacaagct
     6421 attattgatg gtttattaaa aaaaggaaca gtctcgaaag aagaggtgtt cgtttccagt
     6481 ggacattttg aaaatgcaca aaaatttgct gctgaaactg gcgtccatgc atgcaaaagt
     6541 aatcaggaag ttgcatcaca atcagatatc gttcttttag ccgtgaaacc aaaaatcatt
     6601 gaaaaagtat tggaagaact aagagaatat agccagaaag tcttattcat ttctattgct
     6661 tcaggaaaat cattaataga tttagaaaca gcgcttgaag atcctcaggc ctccatcgta
     6721 cgtgtgatgc cgaatgtcaa tgtcagcgta ggagcaggcg tttctgcgat ttgcaaaaat
     6781 gaagctgcca cggaggaaca gcttcagcaa gctacgatga tttttgaagc aatcggcagc
     6841 gtctatcctt tagcagaatc agatttcagt acattcattg gccttgctgg cagctcaccc
     6901 gcattcgttt acatgctgat cgatgcgatg ggacggactg gtgtactgaa tggtttgtcc
     6961 aaacagacag ccactgagat tgctgcaaaa gcaattatgg gttcttgtca aaaattcctt
     7021 tcaagtgata aaaatccatg ggaacttgta gaccaagttt cttctcctgg cggtacaact
     7081 gttgcaggtg tagttgcctt agaagaagca ggattcatcc cagcaatcat caaaggaatc
     7141 aatacgacga tcgagaaaga tcttgagatg ttgaataaaa aataatgata gcaatgtttt
     7201 ttcgtgcctt acaaaaaaag aagagctgtg gcttttgccg cggctcttct tttgtatttt
     7261 ttattattta gcataatcag ttgcacgagt ttcccgaata acgattactt tgatgtgtcc
     7321 aggataatcc agttcttctt cgattcgttt acgaatatca cgtactaaac gaacagcgtc
     7381 taaatccgag atttcttctg gcttgaccat tacccgaact tcgcgtccag cttgaactgc
     7441 gtaacttgat tctacaccgg cgaagctgtt cgaaatattt tccagacttc ttaaacgacg
     7501 gatgtagttt tctaatgatt cacttcgcgc accaggtctt gctgctgaga gcgcatctgc
     7561 tgcagctaca aggacagaga taacagaagt agcttccaca tctccatgat gagaagcgat
     7621 cgcgttgatt actactgggt tttctttgta cttggcagct aattccgcac cgatctcaac
     7681 gtgagatcct tcaatctcat ggtcaagtgc tttcccaata tcatggagca acccggcacg
     7741 ttttgctaat tggatatctt cgccaagctc tgctgctaag ataccagcta attttgcaac
     7801 ctcgaccgaa tgcttcaaga cgttttgtcc ataactagtt ctgaatcgca gacgtccgat
     7861 gatcttgatt agatctggat gaagtgtatg agcaccgact tcaaacgctg cctcttcccc
     7921 gtattcgcga atacgttcat ccatttcttt acgagatttc tcgaccattt cttcgatacg
     7981 cgctggatgg attcgtccat cttgtatcag tttttccaag gtcatccgtg cgatttctct
     8041 tcggattgga tcgaatccgg aaagaacaac tgcttccggt gtatcatcga tgattaaatc
     8101 gataccagtc agcgtctcaa gcgttcgaat attacgacct tctcgtccaa tgatacgacc
     8161 tttcatttca tcattcggaa gggtaacgac tgaaacagtc gtttctgaaa cttgatctgc
     8221 tgcgcagcgt tgaatcgcta atgataataa gtttttcgct ttgcgatctg cttcttcttt
     8281 ggcacgctgt tcagattctt ttaccattag agttaactca tgattcagct cttcttcagt
     8341 cgatttcatg atgacatctc gtgcgtcctc ttttgataaa ccagcaattt tttctagttc
     8401 tttttgttga tcttcaatca ttttttcaac gtctttttct cgctcttcaa ttaattgctg
     8461 ttttgaacta agtgcttctt ctttactttc aagtgagttc tcgcgttttt ctaacgagtc
     8521 atctttacga ttcagcgttt gttcgcgttg aatcaaacga ttctcttgag attttaattc
     8581 taacttactt tctctcagct cactttcaac ttcagaacgg tatttctgat tttcttcctt
     8641 agcttctaac aatgcttctt tttttagagt ttctgcttct ttttttgcac tatcgatgat
     8701 tccagcagct gatgattgtg caccagctat ttctttctcg tgacgtgact ttgcgaccat
     8761 aaaccctaaa ccaagaccga caattaaacc gatgatagcg aggagaatgt tgaataccat
     8821 aaaatccacc tccatactat ctttttcatt tttatcatct atacagcttc tatgataaat
     8881 tattctaaac tcgtcaatat gcatcttcgc atatatgtgt acaaaaaaca tgcacaactt
     8941 tattctaatg tttgtacccc gtagtgtcaa ctttaaataa acaaagaaac ggctttctac
     9001 ttattcatta aacgatggtt tttaaaataa atgacactag tttcatatag gtaaaccttt
     9061 aaattgagaa acttttctat taaattattc tttgtagatg aacagatatt tttattttat
     9121 gtacccactc gtataattaa ataactcacg atttctttat tcaataaaaa aacggcaggt
     9181 caaggagagt acctactctt cagaccagcc gtttttgttt atcataattt ttattcttct
     9241 actaaaggca attcttcttg tcctttttca tcttctggga cagcgacttc ttcaccaata
     9301 ccataagctg ctctaactag agcagaaact tctgccatca tttcaggatg attagccata
     9361 tagattttag cgttttcccg tccttgacca atccgatctt ctttataaga ataccaagca
     9421 ccgcttttat caacaatgtc tttttctact gccatgtcta gaagttctcc ttcttgtgag
     9481 atccctaaac catacatgac atctacttca gcgattttga aaggaggtgc tactttgttt
     9541 tttacgactt tgatctttgt acggttacca acaatatctg taccttgttt caattgttct
     9601 gcacgacgta cttccaaacg gatcgttgcg tagaatttca atgcgcgtcc tccaggagtg
     9661 atttctggat tcccaaacat cacgcctact ttttcacgga tttggttgat gaaaatagcg
     9721 attgtttttg ttttgttgat cgaaccagag agtttacgca atgcttgaga catcaaacgt
     9781 gcttgtaacc cgacatgtga gtcacccatt tcgccgtcga tttctgctcg tggaactaaa
     9841 gcagcaactg aatcgactac tacgatatct acagccccac ttgatactaa agcatcagcg
     9901 atctctagac cttgttctcc tgtgtcaggc tgtgaaagaa gtagttcatc gatattcaca
     9961 cctaattttt gtgcatattg cggatctaac gcatgctcag catcaatgaa agcggccgtt
    10021 ccgccatttt tttgtacttc tgcaatagcg tgtagtgcaa ctgttgtttt acctgaactt
    10081 tcaggtccat atacttcgat gatacgtcca cgggggtaac cgccgacacc taaagcgaca
    10141 tctaacgcaa gagagccact tggaattgta gagatttgtt ggtcgatttt ttcgcccaat
    10201 ttcataatag agcctttacc atagctcttt tcaatttttt tcagtgcagc atctaaggct
    10261 gctttacgat catctgccaa tacgataatc ctccttatat ttacaaatca gagcaacatt
    10321 tcattatcct tatcatatct tttttagtga aaaaaagcaa gaaaaaaccg aacaaatatt
    10381 cgcatttatc attttcttaa aagctgtttt ctcaaaagat caaatccttg cataaccgca
    10441 ttatatctga tagcagcacg atctctggca aagtgatatt tttccgaaac cacgccgtct
    10501 ttagatgaaa tagcgatcca tactgttcct gcagattgac cttctaacgt gtccggtcca
    10561 gccactccgg tgaaagctac actgtaatct gttcttgcta tctcacgtgc ttgaatggcc
    10621 atctgctcca cacattcttt gctgactgtt ccatattctg ctaatagctc cggatcaatc
    10681 tccagaaaac cagcttttgt ttggagcgag taggtaacga atccgcctgg aaatacttca
    10741 gatacaccag caatgtcgcc taatgcggct tggaaggcgc cggctgtcaa actttccgct
    10801 gctgttacag tctgcttgtt ttctttcagt agttccacta cgacttttgc tagcgaatta
    10861 tcatcgccgt atccataaaa atattcccct actcgttctt ggattttttc ttctaatgct
    10921 aaaagagctt gattccctgc ctgaacatca tttgttttca ctgtcagtcg taatgtcact
    10981 tcatttggtt tggcatatgg tgcgatcgtt ggattgatct gtgtctcaat cagatctttt
    11041 agctcagtga ccagtcgcga ttcaccaatc ccataaaagc gcaaaacttt agaaattagt
    11101 ttttcttcag aaggaaaacg ttcctctagt aaaggacgta cttgttccac aaacattggt
    11161 ttcaactcat taggcggacc gggaagaagg atatacgcat gtgtatctgt ttgataaaaa
    11221 gtccctaaag caagccctgt acgatttggt agaggaaccc cgccttcgat aatctgggac
    11281 tgttgcagat tgtttttggt catttttcga tgggttgttt caaaatatgc caataatttt
    11341 ttgtatcctt ccgtattttg tatgagagac ttaccgaggt gagccgcagt tgcttctttt
    11401 gtcaggtcat cttctgtcgg tcctaatccg ccacataaga gtatgagttc acttcttgtt
    11461 tcagctagag caatcaactc ttctaaacgc tgctgattat ctcctacaac tgtttgataa
    11521 taaacttcga tacctagatc agctagctgt tctgacaaga aagtggcatt cgtattcacg
    11581 acttgtccta gtaaaagttc tgttccgaca gctattattt ctgctttcat tacttcccgc
    11641 tcccttatat agtatacgct gtatttttat atttttttat ttaaaaagag ggcaaggagc
    11701 tgtgacaaaa atcttgtcac agctccttgt tccaaataaa cagtgttctc ctgccaaaac
    11761 ctcggtcata agtcacaata tacataaaac atgaaaagct gttttttgaa attatccctt
    11821 gtgactcaga gttagacggc aggctcacaa cctctccact aaacgatact tacattgatc
    11881 ctttgaacac accgctgttt ttgatgaagt aatccacacc tgaatagata gtaaagatca
    11941 gacaagcata aagcatgatt tgatctactg gaataccgat caggctgaat gggatattat
    12001 tgataaacag caagataata gcaaccattt gtgtagccgt cttgactttt cccggccaag
    12061 ctgcagccat gacttctcca tgttcgacaa gtagtaaacg caagcctgtg acggctaatt
    12121 cacgacagac aatgatcgct actacccaac caggggcctt tccttggtca accaacatga
    12181 taaaagcagt cataactagc attttatctg ccaacgggtc cgcaaacttg ccaaaattag
    12241 tgacaaggcc tcgcgcacgg gcaatttttc catctaacca gtctgtgata ctagctaaag
    12301 caaaaataac tgcacctact agctgtgtga cagccagtgt ggtgtctcct acgttaaatt
    12361 gtccccaatc taaaggtaaa cctacaacca aaatgaaaat cgggatcata caaattctaa
    12421 gtacagttaa tttatttggt aaattcacac tatcgactcc tttcttccct attctacgtg
    12481 actatcagcg aagtttccac cgatattatt tacttttcca aatgaattta ttcaaaggag
    12541 tatttttatt gaaattgatc tttttgaatt aacgaagaag gaccgcgaca cagatttgtc
    12601 acgatccttc ttttttatac ctacaagtaa acagatgcgt aaactaattt atacgatcat
    12661 tctgcgtatt gaagagtaag gttcacattt tttggactct gattgttttc ccctggatta
    12721 actggtacgg attctccatt cgctttgatc gttgcattgc ttgctgcacc aatcgtgatg
    12781 gtcgcatttg ttgctgtttc cggtaacacg gtactctgtg tttcatttgc tgccagtgtc
    12841 ccttgataaa cataggcatt gtctactagc acacctaacc atacacgatt gacagcagta
    12901 aattcaaggg ttacaggctt ggtagcgtct gtgatattta ctgcgatggc actgccagaa
    12961 tcttctcctg tagctattgc cattttcttt tcttcacttg attttgttgt tgattctact
    13021 gtagaagact ctttggtctg cgtgctttgc tctgaggaag aggactcctc gactgatcct
    13081 tctactttaa gcgaagaagc tgtgtctccg attattggat cagcttgacg atcttgccaa
    13141 gtcatatagc ccacgacaac gacaatcgcc aaagcaacca atcctaataa aatgacagga
    13201 agacttgtcc aaaagcgact tggatgcgat tcttcctgat gcattgcttt tctagaagta
    13261 cttactgttt caggctgcgg acgttcaggg accgatcctg tcaatttgct tttcccatca
    13321 aaaacatcga ctaaatggtc tccatcttct cctacagctt cagcgtattg ccgaataaat
    13381 gcccgcacgt aaaatttacc aggcaattgg tcaaaatcat cattgtcgat tgcttccaaa
    13441 taacgttttt gaatttttgt aatctgctgt aattcatcta aagatatttt tttattcagg
    13501 cgtgcttgcc gtaatttttc tccgatggtt tcaaaagcca ctcgttcatc ttctccagtc
    13561 tgtttgattc tccgaagcat ctgctgttgc aacgaaagac gttatgtttg ggtgctgtga
    13621 caaaagtcat gtcacagctc cttttttccg aataaacatt gttctcctgc caactcctcg
    13681 gtcacaagtc acaatatcca ataaatataa gaagctgttt tttgaacttg ttccttgtga
    13741 ctcggagtta aacggcaggc tcacgacctc tttcttaaac ggtgttcaat cagctgttcc
    13801 tcggtattag ctcaaaaacc ggcaaaatat aaggagatat ttttccgtat gtttctctaa
    13861 tactcaggtc aaaacgctga tttcacaacc tcttccttaa acggtgttca aaagcttgca
    13921 tctgcggtaa taagtccaac caagttaaaa acattaagaa gctgttttta cttggctgtt
    13981 cttattactt gatgcagggc agctttttca caacctcttg gtacacggtg ttccagaagt
    14041 agcttcttcg gcaataagcc gaaatatcac aaaaatttga gaagcaattt tcgtaaattc
    14101 cttcttattt cttgaagctg accacttctg tcacaacctc tttgattact tcgtttgatt
    14161 ttattttaat ataagttgat taaaagagag catgatttgt tttattttct cactaatttt
    14221 tccaacacaa ctaagaatac cataaaagct gattttggag gaggttccta ctacaagtct
    14281 aaaacgaaat ttaagacttt tcctcataaa atactggttt attttggtct catgtaaaac
    14341 tcgctgaagg cctcttcact tataaatgct tccccagctg ccagtacgtc tttcatttgg
    14401 atcgaatcga tgatttcagg aagatcaaat aaagttcgat cgccaaataa actttgcgta
    14461 aattgattgg caatatattc gatcgaattt aatgattgga aatattgtcc aagcattttc
    14521 ttcttcagca aatcgagatt tgtttctgac acttccggat catcagcgaa tccaagaatg
    14581 atttctttga ctttttctgc tgctttctct ggttcgtccg tatctccgct gaagtccgca
    14641 aagtgaaatt cacgatccaa gctgaattca aaaccaaagc tgtcatcgat aattccttga
    14701 ttgtacatcg ctagatagtt tcgagaagta ttgcctaaaa tcatttggaa caacagattg
    14761 attgctgttt tatagcgtaa cagttctgtt ccttcttgcg gcaatgtgtc taatccttta
    14821 ataccaagta caaatttatc tcgtgtaatg gctgcttcca atgcactttg tttgatgatt
    14881 tcttttgtgt tttcagggaa ataacgtaca atttcttgct ttggagggaa attttttgct
    14941 tcttggtttt cccgaataag tttcatcaat ttctcaggct ccatcttacc aactacaaat
    15001 aaaaccatat tgcttggctg ataaaacgtc cgatagcacg tatacaagtc ttgagctgtg
    15061 attttgtcga tactttcaac cgtgcccgca atatcgatat gcaatgggtg tgtaggatac
    15121 aagttattta gtatgccaaa aaacatccgc cagttagggt cgtcttcata catttggatt
    15181 tcctgaccga tgattccttt ttccttgttt acagtttctt ctgtgaagta aggtgcttgt
    15241 acaaaatcga tcagagtcgt cagattcttt tctacctgat ccgtcgtaga aaacagatag
    15301 ctggttttcg taaaacttgt gaaagcattg gcggaagctc cttgtttccc aaataattga
    15361 aagacatcac catcttcttt ttcaaacaac ttatgttcta aaaaatgggc aattccatct
    15421 ggaactttca ccttttcttt ttcaccatag ggaataaatt cgttatcgat cgaaccataa
    15481 ttcgtactga acaaaccata tgtcttgtga tagtcatttt taggcaacaa atacacggtc
    15541 aaaccgtttg gcagtacttc atgataaagt gtttcattga tttgctcata ttctgtttta
    15601 ttcatttgca tttcctcctg ctaaaaagaa gattgcctgc aattcgattt gctcagctac
    15661 ttgttgtatc tctttgatcg ttacgctctc catacgtttc aaccattctt catcagataa
    15721 ttttgtttcc ggcagccagc tgtccaaaaa agaagcttcg attgccgctt gcggagagtc
    15781 tagagaaagc agatattggt ttctcagcat tgcttttgtc tgagacagtt ccaactctgt
    15841 tatttcacca tttcgcaaac tttctaattg ctcgtgaatc agatgaagta cttgatttcg
    15901 gttcttttca tcgataccgg tttggacact catgaatcct cggaacgtat ccacgctact
    15961 agaggcataa taagctagac tttctttctc tctgacattc atgaaaagtt tagagtgagg
    16021 aaaaccgcca aataaaccgt taaagaccat tagcgcaaaa cgttctggtt catcgtaata
    16081 cacatttgtc tgataagcta ggttcagctt tgcttgaacg attggctcac gtacttgtcg
    16141 ttccttcacg atattcactt gaggttgggt ataaaacaga tctggataaa ctgtttcccg
    16201 cgactcaaat ggtaattgcc caactgcttc agctgctttg tccggatcaa catcgccgac
    16261 aacaaaaatg tcgatttgat catttgccat catttcttga tagtaggacg ctagcgaact
    16321 agcagtgatc ttgtccaaat cttcgactac gcccaaccct gggatctttt gttccgggct
    16381 gttttcaaaa taaagttctt gcaaggcata ggaagcaaaa gtttgtttat cttccttcaa
    16441 actttccaaa tatagacgca gattgttctt ttccaagtca aacgtcaact gatcaaactg
    16501 atgattttta atatttggat aaaataaaac ctcttttaaa aaatctaccg cttgagaaaa
    16561 aaggtttgga tcattgacat attcaccatt cacaaaactg atccctgcgt ttagccaatg
    16621 taaatttcct ttttttctaa ctgataatcc aaagcttgcg ccataaagct ctgcaagttg
    16681 cgcacttaac tttgtctgat ccgaataatt caaactattt gtttccaaca tgctgctgag
    16741 aagtgaacgt ttcgtgatga cttcttgttg cagacgcgca gtaaaacgga taaaaatccg
    16801 gatcgttttg tattgttttg ttggtaagac atgaagattc acgccttctg ccaattgata
    16861 caccatattt atatgctcct tttcacagtt gattgcccgc ttactctttt aatgggtcaa
    16921 cctgcctttt ttacttattc ctctgcttga aatttataag ttcaggcaat tcgccgtttt
    16981 ttctaaatga tgttacactt tatagtatag catagatgtt ttttatcaaa agaaacagac
    17041 ggctaaaagc gtatattgtt tttttatttt ttatttttcg atatactaaa gacaaataaa
    17101 agaagggagg gtcattcatg atcaaagaat tcaaagagtt catcatgcgt ggagatgtct
    17161 tggatcttgc tgtcggcgtt gttattggta gtgcgtttac aagcatcgta aaccaagtgg
    17221 tgcaaggctt gatcacacca ctaataggtt ggtttgtcgc aatgatcaca ggtacttctg
    17281 atttagaagg agcactttca attcttgact ggtcgccgac aaaaggcgtc acatttgcgt
    17341 ttggtaacgt tattagtgct atcattacat ttctgatcac tggttttgtc ttattcttag
    17401 ttgtcaaagc ggcaaaccgt gcgaagaatt tacgctccaa agaagagcaa gtggaagtgg
    17461 aagaaactcc tactgcagaa gattatctta gtgacatacg cgatcttctt gccaaacaag
    17521 tggcagaaga caatacacat ccagtagagc aaacaaatct ttccgatact tctaaataat
    17581 aaagaatcta aaaaaaagaa agccgatttt cagtgtcggc tttctttttt tagattctta
    17641 acttgctact tgttcctttt tcatgtcgat atgtggaata tcatcttcca aatacacatc
    17701 tgagattgcc ttgaatccaa acgactcata aaaagcttgt aggtattctt gtgcgccgat
    17761 cacgattggt ttttctggaa aatttttctg gatcatgtta atcgtttctt gaacaatctt
    17821 tcttcctaaa ccatctttac ggttgtcctg atgaacaagg acacgtccaa aatggatact
    17881 ctccgcttct tcatatactc gcgtgtaggc aaggatcttc ccatcagtat cttgaaaaaa
    17941 cgtatgccaa gcttctccat caatgtcatc aacttcttga taaggacact gttgttccac
    18001 aacaaaaact gcgattcgtt ctttgacgat cgcgtaaaac tctgctgttg acaattcttc
    18061 aaatttcttt acctgatatt ccactaagca aacgccccct gcttttttat tcttagcata
    18121 ccgattcgca cacaaaaaag caaacgaacc ttacgaatct caggctcgtt tgctttttaa
    18181 actgcttcag ttaaaaattc gatctcaact gtccgagtaa gtacatctga atagctgtaa
    18241 gatactcgtt caaaagaatt ttcttcagga tccaaatcaa caacgaatac tgaaggatag
    18301 gtttctgtta aaacgccgcg gcgttctgtt tgacgctttc ttcctgtctg agcaaccaac
    18361 atgattgggc tgccgatacg accttctaaa tctttcttga ttgaagctaa agttgttggc
    18421 atacagttca cctctactat caactatacc acatattttt aaaattttca agtttaccat
    18481 aaaaaaaaaa gaaatgcaat aaaaataaat aagaagctca ttaagatttt tctaaattat
    18541 tcctcattat aaaatttcaa gtgttttttt attttgagtt ttactctccc gtaagcgctc
    18601 attacttgct tgtgatccat tgctaaacat tcagccatgt ctacaaaatc aacgccttgc
    18661 atataataat gaaaaatttt agcttctatt tttgaaaact ggatttcgca attttcgatt
    18721 gcttctttta ataaaagatg ctgaattgct aaaggttctt gcaatgtgat ctctgctaaa
    18781 aagtctggtc cttctgtctc tattttttgg tcgattgaga cttcatcttt ttgatcacga
    18841 cgcttcagcg cgttctgctt cctcaacaga gaatagattt gattgcttat aacgctttta
    18901 taataacttc caaagctaac acctttgctt ttttcgtaag ttctcaatgc ctgatataaa
    18961 gcgatccttg cttcttgttc ccaatcctca gaaacataat ttcttaaata ataggtgttt
    19021 ttcaaacgta gtaaaagtgg aaaatatttt tgatacaggg cagcaaatgc ttcttcgtca
    19081 ccattacgtg cagcgcgcaa taactcctca gacataaaat aattcctcca taaattcgtt
    19141 tatggaggaa tcttatcatc aatgattcat cgaactgatt agaacgatat gttatttatt
    19201 ttttctcatt aaatcgtcca atttttggga caattttttt aactgctctt cattccatgg
    19261 agaattacgt cggaaatcaa taaaatgcat atctttattt tcttgggcta ttgttttttc
    19321 cgtttttttt atcgtcttat ataattcata agcagaagtt cgtaatgctc cttgggaaaa
    19381 gatcatccac tgttctgcta aatcgcttgt cgccactgtc acttgtgtca aacggtcatt
    19441 cagctgacca gcgattctct cgatataact gtcagccgtt tcgtcttctt tcgtaaaaac
    19501 aacatccaac tggtatttct tataattttg ttggatgccc ggaactagtt gggcatcgaa
    19561 gacaacgatg acttccattc cttcatattt cgcataattt gataaacgat gtaaaagagc
    19621 ttctcttgca tccgccattt tgtcctgctt tttaagctgg acaagttccg gccacgaacc
    19681 aatcatgttg tagccgtcta caatcaacag ctgttttttc atggtctact ccccctgacg
    19741 ttgggaaaaa gcctgataca taagcaaacc agcagcaaca ctggcattta agctctggac
    19801 gtgcccggtc atcgggatcg tcagcaattc atccatttct tttttcaatc ctggactcat
    19861 tcctttgcct tcattaccaa tgactaaagc aattgatcct ttggcattcc atttttggaa
    19921 gtttgtgccg gtcatatctg tgccaaatac ccaaaatcct gtttctttga gctgttgcac
    19981 agcttgtacc aaattcgtca cgcgtgcaac agggacatgt tcgactgccc cagtggaagt
    20041 ctttgttacg ataggtgtga ttccaactgc tcggtgtttt ggtatgatca caccatcgac
    20101 gccacttgcg tctgctgtcc gtaaaatcga cccaaaattg tgtggatctt cgatgctatc
    20161 caaaatcaaa aagaaggggt cttctttttt ctcttttgtt cgttcaatca gttggtcaag
    20221 ggataaatat tcataaggtg taatcgcaag aacgactcct tgatgcacgc cgtgatcgct
    20281 caaagtttct aatttttgtt taggaaccca tttgactggc acagcttgtt cacgtgcgat
    20341 ttctttcagt tcttctactt tttctcctcg tgaatcttct tgaagaaaga gtttattccc
    20401 tcgcccttgt tgtagcgctt ctactgcggc atggtgccca aagacgaaat tatcttctaa
    20461 tgcttcttca ttttgatcag aaactgactg atttcgtcca gtttgctttt tatttgaata
    20521 tctattttgt tttttgtctt caaatccacg tttcgattga tgtttgtttg ttctttcttt
    20581 acccattttt ttcaccaact ttctgaatac accagcggat caattcttcc atccgctcgg
    20641 tttgttttgt caaatgcaga tagcccatga gcgcttcgaa ccctgttgcg acccgatacg
    20701 tcgtaacatc agcatttttt gcgctcgtat ggcttttagc atttcttcct ctgcgataat
    20761 aaagttcttc ttcttctgtc aaaagatgtt cttccagcat ttgctgaatc aaaaaggctt
    20821 gtgcattcgc agaaacataa ttcgttgccg cacgatgcag cttattgggt tttgtttctc
    20881 cttgatcaat caggtggtca cgaacataga tttcgtaaat cgcatctcca acataagcca
    20941 aggctaaacc gttcaattgt gtgtaatctc tcatgcttct cttctccatc gggttccttg
    21001 cggtgtatct tctaataaga tccctttttc tttcaacaaa tcacgaattt cgtcgcttcg
    21061 gacaaaatca cgattgcttc ttgcttgatt tcgttcttcg atcaattgtt caatctcttc
    21121 atctaataac tcttctgctg taaataaaat accgaagatc gaaagccatt gcgagaataa
    21181 agcttctcct tgtgccaaga cagcggcaga aactttcggc tggtctagat aagtgttcag
    21241 ccacttagca agctcataga ccactgtgat cccatttgct gcattaaaat catcgtccat
    21301 ttcttcgatg aatcgctgtt ctaaattttc taacaaagcg attttatccg catcatctgc
    21361 caaattctct gaggcagttt cttggcggaa tttcaaattg tcaaacgtat tcttcagtcg
    21421 ttgtaaattg gtggctgctt ctttcatcgt cgtttcactg tagcggatcg gacgacggta
    21481 ttgtgtggtc gccatgaaga aacgcaagac ttgtggatca acgtttttga tcatttcatg
    21541 tacagtgatg aaattcccta gagatttact cattttttca tcgtcttcgc caatcgttac
    21601 atagccattg tgcatccagt aattagcgaa ttttttccct gttttcgctt cactttgtgc
    21661 gatttcattt tcatgatgag gaaattctaa atcttgcccg ccaccatgga tatcgatcgt
    21721 ttctcctaaa tgttttgttg ccataacgga acattcaata tgccaaccgg gacgtccttt
    21781 gccccatggt gaatcccaag aaatctcgcc aggttttgct tgtttccata aagcgaaatc
    21841 taatgggtct tctttttgct gctgttcttc ccctgtacgc tgacttgctc cgacttccag
    21901 ttcatcgatg gattggtcac ttaatttccc ataattttca aatttacggg tacggtaata
    21961 cacatcacct gaagattcat aagcatagcc tttttcaacc aacacttgaa taaatgctaa
    22021 aatatctggc atatgatcca tgactcttgg atgcagtgta gcaggttcga cattcaacgc
    22081 tttcgtatct tcttcaaatg ctcggatgaa acgatcagca acttctggtg ctgtaatacc
    22141 taattcgttt gctgtacgga tgattttatc gtctacatcc gtgaaattcg atacgaaatc
    22201 gactttatag cctcgatatt caaaatagcg tcgaattgta tcgaaagcaa tggtgctacg
    22261 agcatttccg atatggatat aattatacac agtcggtccg cagacataca taccgacatg
    22321 tcccttttgt atagggatga attcttcttt ttttctggtc aatgtattgt agatttttat
    22381 cattctgatc tcctcacttt ttctcctttg attcgaacga cttttgctgg tactcctacc
    22441 gctgtagcgt cgtctggaac atctttcaac acaacggcac ctgcaccgat tttggcgttt
    22501 tttccgatcg ttaccggtcc taatatctgc gcattcgcag agagcatggc tccttgttta
    22561 acagttgggt gtcttttacc cgtttctttt cctgtgccgc ctaatgttac gccatggaat
    22621 aaaatcacat cgtcctcgat ttctgcggtt tcgccaatga cgattcccat tccatggtcg
    22681 atgaacacgc cagttccaat cgttgctcct ggatggatct caacaccagt taggaaacgc
    22741 cagaattgag cattgatctt cgccaataaa aagagccggt gctgatataa aaaatgagag
    22801 aaacgatgcc aaaacaaggc atgaaaacca gggtatgtca aaacgatctc agctgtcgaa
    22861 cgtgcagccg gatcgtttct ctttgctgca tcaatcgctc gggtaaacca actcatgact
    22921 ttccactcct ttcgtaagaa ttttatgtag gaaatcatct tcttataaag aaaagaagcg
    22981 ccttttaaat aagtttcctt atcaaaagac gctttccgcg tggttccact tttcttcata
    23041 atcaatgatt atctctgaca gctaacgcct gttatacgtt cttggctact ttgtttccta
    23101 taaaacagtt tttcctgttt tctgtgtttc gccaagacca ctcccagagg catttcggca
    23161 accattcatt gataccttgc accacccggt atctctcttg taatgatcag ctgcttactt
    23221 ttttctgttc atcgtgttta gttcagtact tgatttaaat gtgctaatga tttttcacga
    23281 ccaagcaatt cgatcgtttc aggcaattct ggaccatgca tttgtcctga tacagctaca
    23341 cggattggca tgaaaagatt ttttcctttt acgccagttt ctttttgaac agctttgatg
    23401 gcagctttga cagttggagc atctactact tccatttgtt ctagctgtgc tttgaatgcg
    23461 tggagaacag taggaactgt ttcaccagca agtacttctt ttgctgcttc attcaataca
    23521 ggatgttcat tgaaaaatag cgtagacaga tcaacgattt ctgcagcata gctcatttga
    23581 ggttgataca ggctaacgat cttcttcacc cattcgattg tcttttcatc tgggttttct
    23641 gctacacgtc catcagcgat tagataaggc agacacatgt cagtaaatgc atctagatcc
    23701 attgctttga tgtaatggtt gtttacccat tgcagttttt tcgcatcgaa agctgcgggt
    23761 gatttactta ggcgttttgg atcgaacatt ttgatcaatt cttggtgttc gaagatttcg
    23821 tcttcgccca caggtgacca gcccaataat gcaataaagt tgaacattgc atctggtaga
    23881 tagcctaatt cgcgatattg ttcgataaat tgcaagattg attcgtcacg tttgcttagt
    23941 tttttacctg tttcactgtt gatgatcaat gtcatatgtc cgaaggttgg tggtgtccag
    24001 ccaaatgctt catagatcat cagttgtttt ggtgtattgg caatatggtc atctccacgt
    24061 aaaacgtgag tgattttcat caaatgatca tcgaccgcaa cagcaaaatt ataagttggc
    24121 ataccatcgc gtttttggat gacaaagtcg ccaccgatat tatcggattc gaacgaaata
    24181 gcgcctttca ccatatctgt aaatgcgtat tctgtattgc gaggcacacg gaaacgtaca
    24241 acagattcta gaccttgtgc ttctttttct gcttgttctt caggtgtcaa gttcgcacat
    24301 gttcctgcgt aatgaggcat ttctcctctt gcgcgttgtg cttcccgttc tgcttctaat
    24361 tcttcttctg tgcaatagca tttgtatgct cgattgctag caagcagctg atcaatcagc
    24421 ggttgataga tttctttacg ttcagattga cggtaagggc cgtattctcc aggattttca
    24481 ggagattcat cccaattcat tcccaaccaa gcaaggtttt ccaactggct tttttcgcca
    24541 tcttcgatat tacgtttttg gtcagtatcc tcaatacgaa taataaagtc tccatcattg
    24601 tggcgggcaa acaaataatt gaataatgca gttcttgcat ttccaatatg caggtgccca
    24661 gttggacttg gtgcatagcg tacgcgtact ttcgtcattt tcttcctctt ctttcaaact
    24721 agactttgac taacagcaaa aattccgtca gttacggctt ctcaaatatc atttatttaa
    24781 agccactcag cacaaatttt accttttcta aggtaaatag taaagccaac tggttgaatt
    24841 tccttaccag ttggctgaat ttctttttta ttttgattgt ttagtctttt tctctgtctc
    24901 ttttttattg tcgattcctc gtcctgcatg tgcaggttta gcaaagatca tgcgtccagc
    24961 agctgtttga agggcacttg ttacaacgac ttcgatacgt tcattcatgt aatgctgtcc
    25021 atcttctacg acaaccatcg tgccgtcatc aaggtaggct actccttgtt ggcgttctgt
    25081 cccggctttt actactagaa cattcatcgt ttcaccaggg atgacgactg gttttacggc
    25141 atttgccaac gcattgatat taagaacagg tacattttgg aattcagaga ctttattcaa
    25201 gttatagtca ttggttacaa tgacaccatc taaaagctta gccaatttga tcaatttgct
    25261 gtctacttca ctgatatctt caaagtctcc atcgtacatt tcgacagata taccctcttc
    25321 tttttgcaag gcatttagga tatctaatcc gcgacgacca cgcacacgtt tcaaactatc
    25381 ccctgagtcc gcgatgtatt gtagttcgta aagaacaaaa ttagggatca tcaaggttcc
    25441 ttctaagaat ccagtctttg taatatcata aatccgaccg tcaatgatca cgcttgtatc
    25501 caagatttta tatttatgga aattttctcc tgattttcgt tctaacagtt gactctcaac
    25561 ttgactagct gtttcttgct cgattttttt gcttcgtgaa ccaaaaactt tcttccattc
    25621 ttcgattcgt gtcgttccca ttctcaatcc cagatagcct aagatgatca tcagtaaaat
    25681 cggcaataca ctattgacga atggaatttc catattgtaa agaggggtcg agatcacaac
    25741 acctaaagtc aatccgatga tcgcaccgat cgcaccgaag agaagataag tcaagctgac
    25801 ctcacttagt ttttgttcta cttgtttgat aatagctgag atatgtttgg ccaacagcaa
    25861 agataataaa aagaaaataa gtgcgcctaa caggctgttg gtaaatgtat tattcagcca
    25921 tccgtttgac tgttgaccga ttgcctgcca agctgagggt aaaaatgaaa tacccaagct
    25981 tgcgccgaca atgatcatca gcaaagtaat gacacgtttt tgcatcgttt ttcctccgtt
    26041 tcgacaatca tttattagtt agcggaacac acgcttcaac gtttcagcca aagttgctac
    26101 tccaactatc tcgattcctt ttggcgcttc ccagttacct aagttgtttt taggtaggta
    26161 tacttttgtg aagccgagtt tttggacttc tttgactcgt tgttcaatat gactgacacg
    26221 tctgatttct ccagtcagtc caatttcacc gatgaaacat tccgttggct gtgttccttt
    26281 ttctttatag cttgaagcaa tactgacagc gatagctaaa tcaatcgctg gttcatttaa
    26341 ttttacccca cctgcagctt ttaagtaagc atcttgattc tgaagaagca acccagctct
    26401 tttttccagc actgccatga tcagagatac ccgattgaaa tcaagacctg ttgttgttcg
    26461 tttggcattt ccgaacatag taggtgtgac taatgcttgg atctctacca aaattggacg
    26521 tgtcccttcc attgccacaa cgatagcaga gccagttgca ccgtctaggc gttcttctaa
    26581 gaaaatctgt gaaggattag cgacttcttc caatccatgc tcatgcattt caaaaatccc
    26641 aatctcattc gttgagccaa atcggttttt tactgctcgc aaaatccgaa aactatgatg
    26701 ttgttcacct tcaaaatata acaccgtatc caccatatgt tctaacattc ttgggccggc
    26761 gatcgagcct tctttggtaa catgaccgac gataaaaatg gctatcccat ttgttttagc
    26821 ggttttgagt agttctgcag tggtttctct tacttggctg acacttcctg ccacacttgt
    26881 gatgtctggc tgcgtcatcg tttggatgga atcaatgata acataatcag gggagatatg
    26941 ttcgatggca cgactgattt cgttcatatc tgtttcagcg tatagataaa attctgtgtt
    27001 gatacttccg aggcgttccg cgcgtaattt tatctgctct gcgctttctt cgccggatac
    27061 atataagact tttccgccta tggctgctaa ttgctgagaa acttgtaaca gcaaagtgga
    27121 tttcccgata cctggatcgc caccaatcaa gaccatagac cctggcacca cacctccgcc
    27181 taacacacgg ttcagttctt ctaattttgt tttgatccga ggttcttttt tagggacgac
    27241 gtctgcgatt tttgttggtt ttgctttttc tcctgtcaaa cttgtccgag ttctgcggtc
    27301 cgttgtatct tgttcgattt cttcgaccat cgtattccat tttccgcaat ttgggcagcg
    27361 tcccaagaac ttaggcgaca catacccgca attttggcaa acaaattgta cttttgattt
    27421 ttttgccatt gtaccgcctc cttttatcct aatcatgaga cattaaatca agcttaagta
    27481 tctttaacgt tttatttact ggaagaacca aatccgcctt gacgttgagc agtcgtttga
    27541 tcttgatctg cttttaaaaa cggcaaaaag atcccttggc cgatacgctc gccttttttg
    27601 atcactacat ccttgaaacc aaagttggtg aattggaaca tgatatggcc ttcatttgcc
    27661 tcattattgt aataatcact gtcaatcaca cctactccgt ttgtcaacac taaaaaccgc
    27721 ttcaacggat tgcttgaacg gttagcaagc tgcaaaaatt catcttctcc catgtaagct
    27781 ttgattccag taggcacgag ggttggtttc aattgttttt cattttctga tacatctttt
    27841 ttcaatccat ctgcgactgt ttccaacccc tttttccagc ctgctttcca gatacttgga
    27901 atcacgacct cttctgctgc ttcaaaatca tagccagcag catgatgtgt agcgcgctgc
    27961 ggtagattga tattttgatc tttatattta gaaataactt caaatcctct ttttctcacc
    28021 ggttttcctc cctaaataaa ctactcctat tctaacatat ccgaggctgt aaaagacttt
    28081 ctttacaaat tcttcaagaa attctgctat cttaaagtaa accattaatt cgttcttttt
    28141 ccaaacgact gcaagttgta caaaaaaact attttttaca tgactttact cgtcaaaaca
    28201 agtagaatag aacgaaatta atgagtggat atttgattga ggtgtcacat gcgtaaagaa
    28261 attaaaaatg caaaacgaat tattgtcaaa gtaggaacaa gtacattgat ttatcccaat
    28321 ggaaatatca atcttgcggc aatcgatcag ttagcttttg tcctttctga tcttagaaat
    28381 cggggaaagg aagtgatctt agtctcatca ggagctgtag gcgttggtat gcataaatta
    28441 catttgtcag aacgtccgac tttgattcct gaacagcaag ctgcggcggc tgttggtcaa
    28501 gccgaactga tgaatatcta taatcaaaga ttcagtgtat ataatcaaca gacggcacag
    28561 atcctcgtga caagagacgt ggtagattat cctgaaagtc gtaaaaatgt gaggaataca
    28621 ttggaacagc ttttgattat ggacacgatc cctatcgtga acgaaaacga tactgtagca
    28681 gtggatgaat tagatcatct gacaaaattt ggcgataatg atcagctgtc agccatcatg
    28741 gcaaaacttg tcgcggcaga ccttttgatc gtcctttctg atatcgacgg gttttattca
    28801 gataatccga ctacaaatcc cgaggctcag atgtatcgct atatcgataa aatcgatgaa
    28861 aagattctgg cacaagcagg tggtgctggt agcacatttg gaacaggcgg tatgcagagt
    28921 aaattaaaag ccgcttctag gatatttgaa gcaaacagcg caatgatttt agcaaatgga
    28981 aaaaatccgg caattatttt tgatattctt gcaggaaaag aagtaggtac tttatttaag
    29041 gaggacttga aatgactgac ttgatgcaat taggacaaca ggcaaaagat agcgcgcaac
    29101 aactagcact tatgcctaca aaccgcaaaa atgagctact tgttcaaatg gcacaaacca
    29161 tcaaaaagaa tcaacaagcg atcattgaag caaatcataa agacttagaa aaagcagtcg
    29221 aaaacaatat cagtgaaacg atgcaagacc gcttgcagct gacacctgag cgaatcaatg
    29281 ccatggctac agagatcgaa aaaattgctt cgttagacga tcctattgga aaagtagatg
    29341 agatgtggac gaacaccgac ggattgagaa tcggcaaaaa gcgcgtgcca ctaggcgtga
    29401 tcggcatcat ttacgaatct cgtccaaatg tcacgacaga tgcagctagt ctagcattca
    29461 aatcagggaa tgccgttatc cttcgcggtg gcaaagaagc attcttctcg aatcaattat
    29521 tagtccaatt attgcagcaa gtactggtca gtgctggcga atctccttat gccatccagt
    29581 ttgtcgatga tacttctcat gaaactgcag gacagttgat gcgattgaca gaatacttag
    29641 atgtgctgat tcctcgaggc ggagcaaaat taatccaacg agtcaaagaa caggcaacgg
    29701 tacctatcat tgaaacaggt acaggaaata atcacgtata tatcgataaa gaagcacagt
    29761 tatctatggc agtgaacatc atcgtcaatg caaaagcttc ccgtccatct gtctgtaacg
    29821 cggcagaaac attgctgatc cattctgaga tcgcaccttt tttcttgcca gctattgaaa
    29881 aagaactggt agaacacggg gtctctttac gtgcggatgc tcgtgcgtta gaatatctag
    29941 agacggctgc tttagcagaa gacacagatt gggatactga atatctagat tatatccttg
    30001 ctgtcaaagt ggtcgattct ttggatgaag caattgcaca tatcaatcgg cataatacta
    30061 aacattcaga aacgatcgtg acagatagtt atgcggctag tcaacgtttc ttaaatgaag
    30121 tggacgcggc ggctgtctat gtcaatgcct ccactcgttt tacagacgga tcggtatttg
    30181 gttttggtgc agagattggt atctctactc aaaagttgca tgcccgtggt ccaatggggt
    30241 taaatgaatt aacttccaca aaatatatca tttatggcga tggacaaatc cgcgaataat
    30301 gtttcgtgtg aaacattatt cccgcctttt gccttttttc tgtaaaggtt ctatccaact
    30361 agaaaggtgt caatatgttt gaaattttaa tgattttttt tatttacctt atttctctga
    30421 atatcgctgc ttttctggga gtcggtattt tatccctttt ctttcaattc aaaaaacgaa
    30481 gcatagggag tcaacgggaa aaatggtcgc agtattttga caaaatcggg caaaaagggc
    30541 tggttacacg cttgcacatt agttacatgg ttgcattatg cttattagcg acaatcaatt
    30601 actattcttt tttcgatcat tcaatcgctt atacgattac gctactgatt gctgggattt
    30661 ttcacttatc ttataaatat cagctgaata aaaaccactt gaatcggact ttccgctaaa
    30721 aaatatccgt acgataagta gagtgccttg aagatcggca aaaaactgcc ggacttttta
    30781 gaggctgtga caaagatttt gtcacagcct ctttttccat ataaacggcg ttcgcctacc
    30841 agctgcctgg taaatctgtt tcctcttcgt taagaagcga aaatccgtca aatgtttcgc
    30901 catcataata acgaagcagc tcatccatgg acaagttcat cgttttttct ttttcttctt
    30961 ccgctgattc agccagtagc tgttcccgat acacctgata cataccagct attttttgct
    31021 gcatcgcttt tctagtttca ccttccgcat tgattattgg caattctttg atatagccac
    31081 gataaatcaa ccgatcatct gaaaattcaa tgggtaattc aacgagtgtc aacccgactt
    31141 cctgaaaata ttcttccagc atctattatc cccccattct ttttcttaaa tgaattttag
    31201 cttacctact gaaaattttc cagtttcatt attttgagaa aaaaagggaa tgtgacaaaa
    31261 atcgtgtcac atcccctttt tccgaataaa tggtgttctt ctgccaactc ctcaatcata
    31321 agtcacaata ttcaacaaac atgagaaact gttttttgaa attgttcctt gtgattcaga
    31381 gttaaacgac agtctcacaa cctcttggta aacggcgttc aatcagctga ccctcggtat
    31441 tagctcaaaa aacggtaaaa tatgagaagc tatttttccg ttttgtttct ctaatactta
    31501 ggataaaacg ctgattttac aacctctgtt aaacggtgtt caatcagctg tccttcggta
    31561 ttagctcaaa aaacggcaaa atatgaggaa ctatttttcc gtttgtttct ctaatactca
    31621 ggacaaaacg ctgattttac aacctcctga taaacggtgt tcaaaagctt gcatctgcgg
    31681 taataagccc aaccaaacga aaaatatgag aagctatttt tgtttggctg ttcttatgac
    31741 ttgatgcagg acagcttttt cacaacctct gatttcttat tcttcttcat ttgtagcaaa
    31801 ataattacgg tgataaatga tacaacatac actgataaga acaatggcac ctgcaaaata
    31861 aaacgcatag ctttcaccaa tatgttcacc aatcacacct gcaataaatg gtgctaaagc
    31921 tccaccgaaa aagcggacaa aactatagct ggacgaagca acgtttcgtt gcagacctgg
    31981 tatttccata gcaatcgtcg tcaacagtgt attgataatt ccttggaaaa atcctgctaa
    32041 cacaatgctg atcggtacaa ggactggcat agaagttcca gctcctaata acagcaaaca
    32101 aataatgaat aagacaaatg ctgctaaaat tgtccgataa gtcgtaaact tccgctcaag
    32161 agccggtgca acgaaaatcg aagcaatagc cagaagcaca ccccagccaa aaaatacaaa
    32221 accgacttct aattcagaat agccaatcaa taaaaatggc gcatacgcaa gtaaagtaaa
    32281 gaatccgaaa ttatataaca atgcgattag tccaactgaa cgaagtttat gattgcttaa
    32341 cgctttgaca ccttcccaaa ggcctacttt tctctttggt ttctcagttt caggtaaaag
    32401 taccgctagg gctataaaac cgcaaaacat caaaaatgct accccaaaaa atggataacg
    32461 ccatgaacca ctgcccaaga ccccaccgac tagagggcca acagccattc cgcagccaag
    32521 tgctgcttca tacatcatga ttgctttttc tgtgttacca actaaaatac tgacgatcgt
    32581 tgctaaagca gtagagacaa ataaagcatt cccgacaccc cagccggcac gtaacccaat
    32641 cagcattccg atattcccag ataatccgcc aagaccagcg aatacaacga tgataaacaa
    32701 tcctgtcatc aatgtttttt tcaaaccgat ccgactagat aagaaacctg taaatagcat
    32761 caccacacca gtgactaaca tgtaactagt aaataacagg gtcgtttcag ctggcgtagc
    32821 gtttaactgt gcagcaatcg tttttaagat tgggtctact agacccactc ccataaaagc
    32881 gatcatacag gtagctgcta caactagcgc tgaacttttc ccctttgttt cttcatttgt
    32941 ttgttcttgt atatctattt gattctccaa tatttccatc cctttctctc tattagcatc
    33001 atacatgatt tattttacat gtcaatatgc acatgtaaaa cgtggaaact ttactgtgat
    33061 ataataaaga aaataagacg agaggtagga tttttatgaa tactttagcc tatatattat
    33121 tatgcgtgct tttatccaaa ccacgttctg ggtatgaatt gaagcagctg atcacaattt
    33181 tttgggaagc ccatcacagt cagatctata caacgcttgc caagttagac cagcagggtt
    33241 atgtgaccgt tttggacaaa gacgagcact ctcaaaaaaa gatctaccac ttgactgatg
    33301 aaggacgtgc tcttgtagag gaatggatca aagaggaaac tcccgctcct acgcaaaaag
    33361 atgaatttct agcaaaaatc tatgcttttt cgacgctcga taaaacaaca gctactggac
    33421 tgctattcac tagagaacaa cagctgaaca aaatcatgca aaaaaatcaa aaaaaattga
    33481 atgacctctc gctagacaag aaacaagaat ttggacgtta tgttgtgatc cagcgcagaa
    33541 ttatgctttg tcagcaggaa ctccaatggt gtcagcaagt aagagaacaa atgcagcagt
    33601 tttttgagta agggataaac agaacctttc tagaaatatc tattgaagag caaattatct
    33661 tcaataaaag agcaatacag ttggaaaaga aaaagcgatc cgaacgacag gccaaaacct
    33721 atcgttcgga tcactatttt tctagaactg cattcaagag actatgacaa aaagcatgcc
    33781 aaggcctttt ttcaaataac agtgttccat cattcattac tttgaattgc ttttagcaca
    33841 cttcttgaaa gcgggccaac gatgatcaac tgcaacggta aagcggcaat cacattcatt
    33901 ttccaagcat tcatataagc agagaataat tgatcaggta ttccttgttc catgatcaca
    33961 ccgaataaag acatacatgt caccattcct aaaaccatca agcatgaaat agttaagatc
    34021 atttggacct tgctttctgg atttactggt agtttaaagg caattttttt cgccagcaca
    34081 ccgatgatga agacatccaa aatgaaagcc accacgaatc caggcactag accaatcaac
    34141 aaacttgata aatgaagatc ttgatgcagc cataaattat acgcgctcat ccccaagacc
    34201 atcaaaaaac acatcattgt tgtaaaaatt attccttctt ttttatttgt aggcaatctg
    34261 taccactcct ttaaatttgt cacgaaaatc atcgtagcac atttaaaaat ttctcgtaag
    34321 tacttttttt attttctgcc cttttatttc ataaacttca tcagccgtac gctcgacaaa
    34381 gtaagaatca tgagaagtaa acaaaatcgt tcccggatat atttgcatta gcttctctaa
    34441 agcttcgatc gttgccatat caataaaatt agtcggttca tctaacagca acacattact
    34501 tggcttagta aaaagcaaag caatcgtcag acgggtcgct tctcccccac ttaatgtaca
    34561 taacggtttc gtcaaagttt cctgagcaaa ccctaagtga ttcagcaaac tacggacaag
    34621 cgattctgaa aactctgttt gtctcatgac aaaacgaatg atggattctt cagatgtcat
    34681 ttgataaccc ttttgctgat atacttgaaa gcttactttc ggagagagaa ggattccttc
    34741 tccttgtttg cggatatgtt ctaataaggt tgtcttacct gaaccatttt caccgacaat
    34801 cgcgattttt ttacctaaag aaaactgaaa atcaccattt accagtaatg ttctcccact
    34861 tctttccaat tggacatttt gtcccatgat gggatagcgg ctgtggagtt caagagattt
    34921 tggtactgga aaacggattt gtttcatctg ctgcggcttg gttgtttctc ctattttttg
    34981 gagtcttctc tctaatgttt tcgcattctt ttggatggct ttttgtacac tgtctttttg
    35041 tttagaggaa gacaaccgat ccagtctgat ttgttgcttt ttctttttac tggacacttt
    35101 ttccaacttt tccgcttgtt ttttcttttc ttggatcgat ttttccaatc gttttttctc
    35161 tttcatgatc gactctgctt ctcgtagctg agtctttttc tccaattcct tttgagtgag
    35221 ataggcacta taattccccg aaaactctcg gatttctccg tcttttactt cccagatttt
    35281 cgatgctagc tcatctagaa aatagcgatc atgactaaca aaaaccagcg taccataata
    35341 ataacgaagc tcttcaatca gtcgatcaat actttttcta tctagatggg ttgttggctc
    35401 gtctagaagc atcccttgtt cataagaaga aagaagacgt atcaactcga tctttcgttc
    35461 ttcccctccg ctccatttcc gctctgaagt ttcttccagt cttaactgga aatgactggc
    35521 taattccaaa tttaggtcct ttgcttcagc gagataggtt aattgaggca ggtagttgaa
    35581 ggtgatctct gtttggattt tcccatgatc gggaaaaagt tcaccggcta ttagtttgag
    35641 caaagtcgat ttaccgacac cattttctcc tatgatccct atacgatctt gctgatagac
    35701 acgaagagaa tcaatagata gttctagctg gttgccaaag ctgacttgta gatttgttat
    35761 atttactgct aaattttcca tatgttttac actcctattt catgtaaaac aacaattatc
    35821 gatactaaaa aagagcagaa aaatctgttt gcaaaaaaac tgcatcacat cttttcctac
    35881 tctgaacgaa ataactatga ctaaatcacc aaatctgtca tagctttcac ctattctact
    35941 gtcgttcgaa aaaatggaca tactccatcc atgaaattgt tgtttataca tgtcttttga
    36001 atttatttag ttgttattca aaagttcgaa acgtaatttc atcgatgcag tcatttttcc
    36061 gacctccttt tttattaggt taccacacac gattcaaaaa aagcaatagc tactgaatag
    36121 taactgaaaa ccttttattc gaacatagtg atcttttctg gtgctaaacg aacagactcg
    36181 taaccttctt ctaccgcttt tcctattgat aaaataataa ctggaacata acggtcttta
    36241 tccaaaccaa atgcctctgc caactgatct gcttcgaatc caccaattgg atttgtttca
    36301 tagccatgat cacgcgcaac taacatgaat tgcatagccg ctaagctaga atcgactttg
    36361 actacatcgt tcatttcttc tcttgaaaaa ctcttgtaat aaggaatgat cgcagctaat
    36421 tgttgatctc tgacttcttt tggcatcttc cctgattcgt aagcttggtt gtagatttct
    36481 tcgccatatt cgtaacattg catatcgcca aaaatcatga ccattgcaga agaagtatca
    36541 ttttgtctcg tattgaaacg gatcaaagga cgcaagattt cttttgcttc ctcgctttca
    36601 gcgaccacaa agcgccatgg tttcatattg acagaagaag gtgcagtcgt cgcttctttg
    36661 atcatttcca gcatttcttc gtgtgggatt ttgtatgttt catcatatac gcgaacggat
    36721 cttctaccag tcataattgt tgaaaaatca tttttttgtt ccattattat taacctcttt
    36781 cgtttctgtt gttatattgc aaagttgggt attcaattta tctaaaagtg ttagaaattg
    36841 tttcatttct acatcgctga aagccgggga tagcacggaa cctgtatctt tatggtccat
    36901 ttcacaatgc ttgattttct tctgggcttt ttctgttagc gctacataca cttcccggtt
    36961 gttctgagga tttctctgac gcgaaacata gccatttgct tccaatacct tcagatgacg
    37021 agtaactgct ccttgatcaa tctgcaactt ttcttgcaaa ctgctttgta gcaatggatt
    37081 ttcttctttc agtctcatca tgatctcgta tctagtaaga cttataccga tattttcttc
    37141 aaagatctgg acgatttttt gatcggctaa tttgatttga tataaaaggt gactaatctc
    37201 ttctaaagac ataatttcct cctttctaat aattgactcg tcaacgattg acttgtcaat
    37261 aatagaataa aaaaagaaaa aagtcaaaga atcagctgtg taataaaaaa aacgctctca
    37321 atagaaaagt atagagaaaa gaaatttttt gtagaatgaa cggaaaaaat gataaagaaa
    37381 gcacaacgct aaaaccaaga ttattgattc ttatcaaact atagaagata gctcttctgc
    37441 agtagataat tttacaccgc caaaaatttc cgaaatcgcc ttttgcaaga atcccgtcga
    37501 atattagaaa ttggatcagt atgcttaaac agtaataaaa aagttatgac aagatgttgt
    37561 cacaaccttt tttgagtaaa cggtgttcaa aaacttgcat ctgcggtaat aagttcaacc
    37621 aaacaaaaaa catgaaaatc tgtttttgtt tggctgttct tactacttga tgcagggcag
    37681 ctttttcact acctcttttt aataaccata aaatcctatt ccttcatatc tccagcccat
    37741 cggtgttagc caatctcttt ccttagcact caaggtatag tgatgggtac cagttttttc
    37801 atttggatta aatagtcgat aaattggctt ggaaccacca gaataaaaac ttagaccttc
    37861 ataacgccaa ccgatttttg ttaaagagtt gatctcagat tgtgccattg tgtaatgatg
    37921 gtctccactg taaggattgt ataaacggta gacaggttgt ccgctgtttg gtgcttgcca
    37981 agcaatccct tcatattccc agcctacagt tggcagataa tttttttctc cggcattcaa
    38041 tgtatataaa tgttctcctg tatgacggtt atacagacga tacatcggag aagttgaagt
    38101 tggtgctgta tcatactgtg tcagattata ggtttcgatt acattgttca atgtagaacc
    38161 ataattaggc gcagtggcat aacgacctgt tagccaagcc gtagcatctt tgtaagagtt
    38221 cgtattgctt ttccatgcac cggaatagta gtaaacgcct ggctgaaaag aagtcgtctt
    38281 caataccgtt acgttatctt gcaatgattc tttgtacgaa ggatattttc taaaagcggc
    38341 attcaccgta atccattgcc cattcacata ttccgaagta ggcatcgtaa cagattggcc
    38401 attatagctt cctttaatcc caaatagatt gtaattaggg gcagaagcca aagtactgtt
    38461 cccccagcca ctttcaacga tggcttgagc aatcatgact gatgcatata agtcattggc
    38521 acttgccaaa ctttgtgcag ttgaggcaat actatcaatg aacgcttgtt gagaattagc
    38581 agatgagcga gcataaaaag catagccagt atagttttcg aattcagaca aatctgcttg
    38641 atgggtatgt gtcaatgttt gcccatctgt caattggttc gtgatttctt ctgcattctc
    38701 tggattgttc tgagcttctg ctgatgtact gtcttcggtt tgtgtttgtt ctgttgtttc
    38761 agttgttggt tcttcggttg aaattgtatt ttctgagaca gtagctgact gcaaagtact
    38821 ttctgtatcg ttttcaattc ctgtttctga agaactcgat gtctgagttg cgacagtttc
    38881 tgtcgattct tcagctagca ctgttgtgtt ggcaactgct agatttccta aaagaataac
    38941 gctaaataat aaattctttg ctttcatctt ttttctcctt tgaattatga aacttttcct
    39001 aaataacagt gtaactaaaa aagtaaaagc aagtcaatgt ccatatttaa ataacatttg
    39061 tttcttttta ataaatttgc tttttctgca taaaaagacg taaatgaaga atattcttct
    39121 ccattcacgc cttaaattga tggtcgtttg aataactatt taacaagtaa ttctctctgg
    39181 atattatccg ttatggatta tacttcctgt gtcgtcggac ggataatcat gttgctgaca
    39241 gacatccggt ctggcgtttc tatcgcaaat gctacagcag aagctatatc ttcagatgtt
    39301 aatccccatt ctttttgcgc ttcatgaagt tcaagagcag cctttttgtc gctgatggtt
    39361 tgatataatt cagtttgaac agctcctgga gagatgatcg tagatttgat gttattttgc
    39421 cgttgctctt gacgtaatcc ttccatgatt gcacggacag caaattttgt tccgcaataa
    39481 acggcggaat caggataaac aacatgtccc gccacagaat cagttgaaat gatatgccca
    39541 gatttttgtt tttccatgat tggaagtacg gcggaaattc catttaacac acccatgatg
    39601 ttgatatcaa gcatattttg ccattcatca cggtgacctt caactaatgg agcggttggc
    39661 atgattcctg cattattgta caatacatcg attcgaccga acttttccat cgctaaatcg
    39721 ataactgctt ggacttgtgc aaaatctctt acatctgctg tttgaatata tagctcagct
    39781 tctggtaaag attcttttat tgcttttagg cgatcttctc ggcgtgcagc tataactagt
    39841 ttagcccctt tttctgctaa tagcttcgtt gttgcttcac cgattccgct tgaagcgccc
    39901 atgatgacaa tcactttttc tgataaatca ttcattctat tctcctcctg ttttgaaaaa
    39961 tctcttgctt atactcacta tctcttattt catcttactg ttcaaataat ccgttcgaca
    40021 ggtttgacga ttttggcagg gattcctgca acgatggtat tgtctgggac atctttcgta
    40081 acagttgcat tcgctgcaac aatggcattc tcgccaatcg ttacgcctgg cagaatagtt
    40141 gctcctgctc cgatccaagc atttttcttg atgtggattg gctttactga caagcctctt
    40201 cgttcttttg gatcggttag atggttcacc gtgatgaggt tgacacgagg accaatcaat
    40261 actgaatctt ctaaacagat gccacctaag tcaacgaaca tcacttgacg attgataaag
    40321 acatttttcc ctatactaat atgtttgccg aaatctgtat aaaaaggcag cagaacagtc
    40381 accgtatcat ctactttgtc actaatgata ttttctagcc gtttgcggat atctgctggt
    40441 tcatggtacc cagtattcag cgctgctaaa ggctcttcgt tttccgcttg gatctgatgg
    40501 atcacttcaa agagcggatc cgttactcga atctctttat tgacgatttg tttttcgatg
    40561 aggcttgtca ttttgctccc tccttttatc cttactttat aacggacggc ttatgatgtc
    40621 taatacttat aaaacatact taactattcc ttttaactat acttagtggt atgctttttt
    40681 aaaaacagga gggaaaaaat ggatattcgt atactgacgt attttttagc agtagctaga
    40741 gaaaaaaata tcagccgagc agcaaaatct ctgcatatca cgcaacctac tctctcaaaa
    40801 caactgaaag atttagaaga agaacttggt gtgacacttt tcactcgagg ctcccgagag
    40861 atacagttga cggattctgg tcgatatctt tattcaaaag gaaaagaaat cttgtcgttg
    40921 gtagaaaaaa cggcgaacaa tttaacaaat gacgcaatca tctctggaga aatctacatt
    40981 ggcggaggcg aaactaaagc aatctctttc atttctgata gccttcattc gttgatcaag
    41041 gcgcatcctg atatccagtt tcatttatat agcgggaatg cagatgatat catagacaaa
    41101 ctggatagcg gattacttga ttttggactc gtaatcgaac cgacaaacaa acaaaaatac
    41161 cagtcatttc gactgccagc ccaagatcgt tggggacttt tgatacatcg gaatcatcct
    41221 ttagcaaagc aaacagtcat ccatccgcag gaactgaaaa ctgttccttt gatcatttcc
    41281 cggcaaacgt cagtggacag tcaactagct gaatggctag gtttcagcat agatcagtta
    41341 tcgatcgtcg gaacatataa cttattgtac aatgcttctt tgatggtcga taacgggact
    41401 tttggtgcat tagctctaga tgggatcatc aatacttatg ggacggaaaa agtattcatc
    41461 ccactcgatc cgccattaca agcttcactg aatctcattt ggaaaaaaga ccagcctctt
    41521 tctcctgctg ctcgtaaatt tttagatgtc ctaacggaga aatacagtta agtagagttt
    41581 ttttccttcg ctttcagctt tttaacaacg aataagttaa gcaaaagatc caatgattaa
    41641 ataacataag tttcttaatt tgttccttcc ttttatttct gataattcca gtaaattact
    41701 ataattataa aaaaagaatt gacaaaacat aactgtctat ataaaattga ttacgttaac
    41761 aataattcgt ttcatgtgac tagatcatac taggcatgaa aaatctctga ggaacatggt
    41821 ttccctagga ggtttttcgt gtctattttt gtttggccgc aatcaactgg tctagcaaac
    41881 gtgaaaacca aatcttcaaa aagggtgcgc gcatgaaaat caaaaagatc tttaatcaaa
    41941 atgcagtatt ggtagatgac ggtggacagg aaaaagtagc tattggaaaa gggattggat
    42001 ttgacagaaa aagaaatgat ttgatttttg atcgtgatgt cgaacggata tttgtcatgg
    42061 aaccagaagg tcaaatcaag ttgcaaaatt tacttaatca gattgatgaa cgctttttat
    42121 ttgcagcaga aacgatcatt gatcatgcgg aaactgtttt gatggaaaag ttgaatgaac
    42181 atgtattgat cgctttgacc gaccacattg ctttttctgc ggagaacatt aataacggta
    42241 tcgtcatccg caacaaactt ttaagagaaa tcgaagtgct ttacagtgat gaattcagta
    42301 ttgctcaatg ggcagtagag tatctgacaa aagagcttgg gattccatat acttatgacg
    42361 aagcaggata tatcgcaatc catattcaca gcggacgaag cggtcggaac aataaccatc
    42421 gcagtatccg tgaagtgacg attgtatctg acatcatcca tttagtggaa tcagaattgg
    42481 aaacagatat ccattcagaa ccattttcac tgaattattc aagactggtg aatcatttac
    42541 gactgcttct acaacgaacc catgcgcaac aatatgctgt tttagacacg gaaatcgtag
    42601 aaatggtcaa acgaaaatac ccagaaagct ataagatttc taaaaaaatc cgtgtgttgc
    42661 tgaccaaaga ataccagctc gcgatcacga aagaagaact gggttacttg gccatccata
    42721 tcgagcgttt aagaagcgca atcgtacaaa caaatgttaa caatcatgag gaggaaaaaa
    42781 attgaaagca tatctacaac gaatgggacg ttcccttcaa cttccagttg cggtcttacc
    42841 tgctgctgct ttactagtag ggatcggaaa ctggtgggca tcttatagta acgacattgt
    42901 cgctcatttt ttacaagctg gcggtaacgc agttttaggt caattacctt tgctgtttgc
    42961 cgttgggtta gcactaggga tgtcagtgga caaatcaggt gctgctgcct tggcaggttt
    43021 ggtcgcattt gaagttccag taaatgtttt aaaaactgat tcagtagcta ctttattgaa
    43081 tatcaaacca gaatcagttg atccatcttt tgctcagatc agtaacgtat ttattggtat
    43141 tattgccgga ttgattgcgg cagctcttta taatcgtttc cacgaagcaa aattaccgat
    43201 ggctttatct ttctttagcg gcaagcgtct agtgccaatc ttggctgcct ttgtcatgtt
    43261 gctgatcagt gcagtgctac tgctcgtatg gcctccggtt tactctgctt tagtttcttt
    43321 tgctaaattc attttgagtt taggctgggg tggtgcaggt ctttatggct tcttcaaccg
    43381 tttactgatc cctacaggat tgcatcaagc aatgaactcc gtgttctggt ttgacgtagc
    43441 aggaatcaat gatattggta aatttttggc ttctgaagga acaaaaggcg taacaggaat
    43501 gtatcaagct ggatttttcc caatcatgat gttcgggctt ccagcaggag catatgctat
    43561 ttacagaaat gcacgtccag aaaacaaagc aaaaacagct tccttgatgt tagcagcagc
    43621 ttttgcttca ttctttactg gtgtaacaga accacttgaa ttctcattta tgttcgttgc
    43681 ttggccatta tatgtcttgc atgcaatttt cactgggatt tctttagcag tgagtgcatt
    43741 cttccattgg acagcaggat ttgcctttag tgccggattt gtcgattact tcctatcttt
    43801 gaaaaatccg attgcgaatc agccattgat gttgatcgtc caaggattag taacagcagt
    43861 gatttactac tttggcttta actttgctat caaaaaattc catctgatga ctcctggccg
    43921 cgaggaagca gatctttcag atgacgatac tgcaacgact aattcagaca ataaatatgc
    43981 ggcacaagca agcaaaatct acgctgcatt aggcggagcc gacaatgtaa cttcgatcga
    44041 taactgtact acacgccttc gtttgcaagt caaagataca ggcctgatcg atcaaaataa
    44101 aatcaaagcc actggcgtac ctggtatgaa gatcatcgac ggaaaaaatg cgcaagtcat
    44161 cgtaggtaca gaagtacaat tcgttgcaga tgagatggca aaactacatg gtggtgctgc
    44221 aacacgacct gcacaaacaa acaccgtggt taaaacagaa acatttgccg gtggaacgat
    44281 cgaacaagat atttacgcta tcgcaaatgg gaaactgatt ccaatcactg aagtttcaga
    44341 tgatgtattc gcggaaaaaa tgatgggtga cggctatgct gtattaccag aaaatggaga
    44401 aatcttctct ccaatcgctg gtacgatcac aaacattttc ccaacgaaac atgctgtcgg
    44461 aatccaaaca gatgctggta tcgaagtctt gcttcatatg ggcatcaaca ctgttgactt
    44521 aaaaggcgaa ccattcactt tatatgtaaa agaaggtcaa aaagtcgcaa gaggtcaatt
    44581 gattgcttta gttgatcttg ccgctattca atctgcaggc aaaaacacgg atatggtcgt
    44641 tgtattcaca aatggcgaca aagtccaaag cttagaaatc gaacccgcac gtgatgtaaa
    44701 agcaaatgat aaaatcggct ctgtttccaa caaagcctga gccatcgtct tccccagact
    44761 tttttatcga taaacaaaca ggagctgaga caaaaatctt gtctcagctc ctttttccga
    44821 ataaaaacgg cgttctcctg ccaactccgc ggtaataagt ccagccaaac gtaacatatg
    44881 aagagctatt ttttatttga ctcttcttat tacttgatgc agaacagctt ttttacaacc
    44941 tctgataaac ggtgttcaaa aaccagttta tcggtatgaa gtcaaattta tgcaaaaatg
    45001 tggaaagcca ttttcacaaa atttttcttc atactcaaaa ctaatcggct ttttcacaac
    45061 ctctttatac attattcctg atccaatatt tcttttaatg ctgcttcaag agaagttaat
    45121 ggttttccca atactttttc taaatcatca gaggaaatag ctaactgatc ctttttaata
    45181 tcatgttgga tcgctaaaaa catgtcagct gcttcttgag ggaatccggc ttcttttaga
    45241 ttttcgacaa acgctgcatc acttgcttca ataatttcta actttttacc tgtcgcacgc
    45301 tctaatgcct tggctaattc ttcatagtca gttagattgc cggataattc taagatttct
    45361 ggagagtcac tttctactag tacttttgct gcagcttctg catattcacg tttcaatgcc
    45421 caacctgttt ttccatttcc tgcagcataa acgaattgac cagtttttaa agcctgccca
    45481 atgataggta attcattctc tacataccaa ttattccgta aaaatgtatg ggaaatacct
    45541 gaatccttaa tcattttttc agtgaattgg tgatctggcg ctaatacact gatcgagtga
    45601 tcggcatctg caaaacttgt ataagcgata taagataccc cagctgcttg tgctgccttc
    45661 actacattct tatgttcggc ttgacgattt cctggaacgc cagagacaaa aagcaatcga
    45721 tctattcctt tcaacgcttc tttcattgag tctaaatcat cataatcacc aatacgtata
    45781 ttaaatccag cttctttcag tgcttctcct ttttcttcac tacgtactag tacatagatt
    45841 tcattttgtg gaaccatctt ttttaagaat tccagcgcat aaccaccaaa acctccagtt
    45901 gctcctgtta ctaaataatg catatttgcc actccctatt ctttttattt gttaagattt
    45961 acagctgtac atttataaat tacaccttta tcataaagag tgtggtaaga taagtcaagc
    46021 aaaaaaaatg aaaggatgac aactgatgaa gtattctctg caatttagtg atgcaatcca
    46081 tgtacttgcc tatattgaaa tgtttcaaga tacccgatca ctttctagtg agatgattgc
    46141 cagtagtatc gaaacaaatg cagcaaatgt acgtaaaatc atgagtcagc tcaaaaaatc
    46201 cggtttgatc tttacaaaaa ccggaaaacc gcagccttcc cttgccaaaa gtccagatca
    46261 gatttcttta ctagatgtct atcgcagcat cgaaggtaat acacagctta tccaagtcga
    46321 tccaaaaaca aatccaaatt gcgtcgttgg tgcgaatatc caagaagctt tagaagaaag
    46381 ctacgctcgg ctgcagaaaa aggcagaaga agaaatggct aagatcacac ttgatagtct
    46441 gatacataaa atagccgttt tggaaaaaga aaaaagacca gaaaacacag gcctcgtaga
    46501 gagattttta taattctctc tactgtgcct gcatttttct gatcggaatc gtctacataa
    46561 attcttttat tctccggggg acaaaagcct tgtcatgccc ctttttcctc tctaatgctc
    46621 aggtcaaagc gctgaagtca tgacgtcttg ataaacggtg ttcaagtagc tgaccctcgg
    46681 cattagttct gaaaaatgca aaatatggaa agctatttcc catttttcct ctctaatgct
    46741 caggtcaaag cgctactttc acgacctcta tattatcatg gtttattgac taccggtatc
    46801 cagatttctc ccatatattt cccgttggta actcccattt cgactacagc atttggaccc
    46861 ccaacatagg cgtaatcttc tacttctctt aaaacatgcc caaaggccgt attcgtcaaa
    46921 tcatttctta attcttcagg cgattctgct aaagcgatta caacaagata cgttcctttt
    46981 gggaatggga tgattcgggt agtgtttgga acatcctgac tcgtttgaac accgacatag
    47041 tacatcattt tcccgttata gggttcgttg actgtaaaga aataatgatt ttcagcagct
    47101 tgtttcaatg catctattgt tccattaccg ataacttttt gctcaaatgc ttgtttctct
    47161 gcagccaaac cgacaaaatc cgtattgcta ctcttcaatt ccacaccaat acccgtcacc
    47221 gtaaaatctg gtttttcttc gatcctatac tttgccattt ctcccaccct ttctattata
    47281 ttaagttcat cttacataaa attaaaggga aatgacacga ttacgctctt ttttctaggg
    47341 atatttttat tttttaacta tttttttgct ttcactgcaa tccaaatctc actgtaaaca
    47401 ttactgaaat cctctcccca ttttgtaaaa gagatctcgg gagcttcgac taactcataa
    47461 tctgaagaag gaagccattc tgcatagatt ttcccccaag tatcttgtaa agtttgggga
    47521 aatggccctt tattaggaaa aatggcccaa gtgtgttctt ctatagcaag catttctaaa
    47581 tcagcgtacg gattttcttt tgtcgtagca aagccaatca tgtgcatcaa actgcccttt
    47641 tcttccaagc gggctccatc aaatccataa gaaacgttga ttacttgatt tggatataaa
    47701 tctcctaact tatgcatctc ttccctttga gtctccgtga tcgtctgcgc taacttttgt
    47761 atttcttgat tgactccttc aaatttgaca ggaacttttt tcgccacacc tgccacataa
    47821 aactgttctt tcttttcgat acgaaattcc atcgattttc ctcctctgac atcaataaaa
    47881 aatgtcaagc gtggaaaaga tttttgtatg ccgtatctgc tgatttctga aggcagatat
    47941 cctgaccaat cacgaaaagc tcttgaaaaa ccttcgactg tttcatatcc atatctaaac
    48001 gccgtttctg tcactcttga tccatctagg agatcgcgat tagccaaagc caatctacgg
    48061 tttttgatat agtccattag cgacatccct gcaataaacg taaagatgcg ttttaattga
    48121 tattcagaga ttcctgtatt ttcggctatt attttatagg aaagtccttc tgtcaaatgt
    48181 tcttcgatgt aatcgatcaa ttggttcaga tctttaagca tatttatctt cctccttgct
    48241 tgccttttta tcatattatc aaagaaagtc aattacttga tgaatcagga accaatttag
    48301 tcagatgtag aaaatgttgt atggatctaa agaaaattct caagcagcgt ttcgtaacgg
    48361 ataattggta ttatctgcct atctatgttt tacctttaga aaaataaatt ttggggaaga
    48421 cacattctaa gtaaacaaag cggtaaaaat atcttttatt cccttagtta atccgcttac
    48481 tacctactgt acaaagcaaa ttgttgttca aactgctgat cagaacgctg cttggatttt
    48541 tctttgcgat acatcatacc tcttttatca attggaacaa ttatttattt ctaacaaaat
    48601 cacttcattt ttgtcagata aataaaaaac aaaaaaagtt ccatctttaa aacacgctat
    48661 tcttttactt tttctcacat tctctaagaa aagaaaagga gaatgaattt ttgacaattc
    48721 gagcaaaagt gattatcaaa tatgtaaaat cagctacttt tttcttaaac gtttctttat
    48781 actaaatata aataagtgtt tcatgaaagg agaactagat gaataattgg cttgagtatt
    48841 ttcctgaaaa cgttttggaa cgcggttaca gctatcatct gcacggtttt gtccgtcatt
    48901 tgaactatac aagcaagtac ttgtccgcaa cagtttctgg cacggaagat tataaggtag
    48961 tgatcacttg ggatgaaaag accaacatga cttgtgattg tctatatgcc atagaaggaa
    49021 aaaaatgcaa acacatggct gctgtcttat tcgcttacga agaacgtcca ataaaaaaat
    49081 ccaactactc actttccgaa ctctcgtccc ttgtttcctc cgctagttcc tctcttgtac
    49141 gggagctttt aacagagatt cttatagaac atccgcaatt tatcgaacgc ttcaaagtta
    49201 agatgccatt tcatgcaata aattattcag ataaattaac aactatcatc cataaatacg
    49261 atcatattat caagaaaaac aaaaacagga aaacagcgaa gttcatcatg gagatgcgaa
    49321 agttcatcca agaagcagtt gaatcgctca ttcagcaaaa tgcctatctt cccgcattcg
    49381 aattgatcaa tgaagtgatt gctactctcg aaacattcta ttgggagcct gaggatgaac
    49441 gaacccttct tctgatagag gactgttatt atttatggaa agaattatta gcagaagcac
    49501 ctcatgcaga aaaaaggcaa atgttctctt ggttcgtctg ccaagtcgat catactgacg
    49561 cctcttattc aaaacgctac tccataaaaa tcctgaaaga agactttcgg gaaaaagaat
    49621 tttctaacca aaagaaaaaa atagataaaa aaactaaaga aacggtaaag aaagacgatt
    49681 tcaatgaaaa atagcaagta aacgataaca accatcctaa atccacaaga aatacagcag
    49741 accttgcttt aaaaacacga atcatgcaaa ggatgaagta aagaagactt aacgcgtacc
    49801 caatattagt catttgacta ttaggtacgc gttttgtttt ataagtcatg acaaatttcc
    49861 ttcgttgctg ggtagatttt ccggcattgt tgatagattt tatgatactt ttctacattt
    49921 tgtaagagag gttccactgc atctgtatat tggataaaat tctgacaaca actttcaggt
    49981 gtgtcgaacc actctaatct ttaaagataa
//
LOCUS       DAZBSA010000007        47496 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000007 DAZBSA010000000
VERSION     DAZBSA010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.007"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..1368
                     /locus_tag="ACHGMT_000385"
     CDS             28..1368
                     /locus_tag="ACHGMT_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN6834161.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1586..2029
                     /locus_tag="ACHGMT_000386"
     CDS             1586..2029
                     /locus_tag="ACHGMT_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFN6834162.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2533..3951)
                     /gene="nhaC"
                     /locus_tag="ACHGMT_000387"
     CDS             complement(2533..3951)
                     /gene="nhaC"
                     /locus_tag="ACHGMT_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFN6834163.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4354..5577)
                     /locus_tag="ACHGMT_000388"
     CDS             complement(4354..5577)
                     /locus_tag="ACHGMT_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFN6834164.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5608..6447)
                     /locus_tag="ACHGMT_000389"
     CDS             complement(5608..6447)
                     /locus_tag="ACHGMT_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFN6834165.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6464..7144)
                     /locus_tag="ACHGMT_000390"
     CDS             complement(6464..7144)
                     /locus_tag="ACHGMT_000390"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFN6834166.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7146..8219)
                     /locus_tag="ACHGMT_000391"
     CDS             complement(7146..8219)
                     /locus_tag="ACHGMT_000391"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFN6834167.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(8275..8513)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8556..8789)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8866..9537)
                     /locus_tag="ACHGMT_000392"
     CDS             complement(8866..9537)
                     /locus_tag="ACHGMT_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN6834168.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(9624..12272)
                     /gene="mgtA"
                     /locus_tag="ACHGMT_000393"
     CDS             complement(9624..12272)
                     /gene="mgtA"
                     /locus_tag="ACHGMT_000393"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFN6834169.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(12614..12788)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            12961..13398
                     /gene="ndk"
                     /locus_tag="ACHGMT_000394"
     CDS             12961..13398
                     /gene="ndk"
                     /locus_tag="ACHGMT_000394"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFN6834170.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(13420..14286)
                     /locus_tag="ACHGMT_000395"
     CDS             complement(13420..14286)
                     /locus_tag="ACHGMT_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFN6834171.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(14446..16539)
                     /locus_tag="ACHGMT_000396"
     CDS             complement(14446..16539)
                     /locus_tag="ACHGMT_000396"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFN6834172.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(17288..17911)
                     /locus_tag="ACHGMT_000397"
     CDS             complement(17288..17911)
                     /locus_tag="ACHGMT_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834173.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(18070..19467)
                     /locus_tag="ACHGMT_000398"
     CDS             complement(18070..19467)
                     /locus_tag="ACHGMT_000398"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN6834174.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(19499..20470)
                     /locus_tag="ACHGMT_000399"
     CDS             complement(19499..20470)
                     /locus_tag="ACHGMT_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN6834175.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            20844..21269
                     /locus_tag="ACHGMT_000400"
     CDS             20844..21269
                     /locus_tag="ACHGMT_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834176.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(21757..22458)
                     /locus_tag="ACHGMT_000401"
     CDS             complement(21757..22458)
                     /locus_tag="ACHGMT_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFN6834177.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(22474..22914)
                     /locus_tag="ACHGMT_000402"
     CDS             complement(22474..22914)
                     /locus_tag="ACHGMT_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFN6834178.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(22914..23489)
                     /locus_tag="ACHGMT_000403"
     CDS             complement(22914..23489)
                     /locus_tag="ACHGMT_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFN6834179.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            23730..23939
                     /locus_tag="ACHGMT_000404"
     CDS             23730..23939
                     /locus_tag="ACHGMT_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFN6834180.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            complement(24337..24849)
                     /locus_tag="ACHGMT_000405"
                     /pseudo
     CDS             complement(24337..24849)
                     /locus_tag="ACHGMT_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(24975..25676)
                     /locus_tag="ACHGMT_000406"
     CDS             complement(24975..25676)
                     /locus_tag="ACHGMT_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN6834181.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(25784..27227)
                     /locus_tag="ACHGMT_000407"
                     /pseudo
     CDS             complement(25784..27227)
                     /locus_tag="ACHGMT_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            complement(27241..29106)
                     /locus_tag="ACHGMT_000408"
     CDS             complement(27241..29106)
                     /locus_tag="ACHGMT_000408"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFN6834182.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(29227..30077)
                     /gene="licT"
                     /locus_tag="ACHGMT_000409"
                     /pseudo
     CDS             complement(29227..30077)
                     /gene="licT"
                     /locus_tag="ACHGMT_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            complement(30387..31100)
                     /locus_tag="ACHGMT_000410"
     CDS             complement(30387..31100)
                     /locus_tag="ACHGMT_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFN6834183.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(31168..31494)
                     /locus_tag="ACHGMT_000411"
     CDS             complement(31168..31494)
                     /locus_tag="ACHGMT_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFN6834184.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(31617..31844)
                     /locus_tag="ACHGMT_000412"
     CDS             complement(31617..31844)
                     /locus_tag="ACHGMT_000412"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFN6834185.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(32166..33419)
                     /locus_tag="ACHGMT_000413"
     CDS             complement(32166..33419)
                     /locus_tag="ACHGMT_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFN6834186.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            33480..34220
                     /locus_tag="ACHGMT_000414"
     CDS             33480..34220
                     /locus_tag="ACHGMT_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFN6834187.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(34306..35064)
                     /locus_tag="ACHGMT_000415"
     CDS             complement(34306..35064)
                     /locus_tag="ACHGMT_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFN6834188.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(35061..35525)
                     /locus_tag="ACHGMT_000416"
                     /pseudo
     CDS             complement(35061..35525)
                     /locus_tag="ACHGMT_000416"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            complement(35544..36041)
                     /locus_tag="ACHGMT_000417"
     CDS             complement(35544..36041)
                     /locus_tag="ACHGMT_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFN6834189.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            complement(36109..39276)
                     /locus_tag="ACHGMT_000418"
     CDS             complement(36109..39276)
                     /locus_tag="ACHGMT_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFN6834190.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(39500..40384)
                     /locus_tag="ACHGMT_000419"
     CDS             complement(39500..40384)
                     /locus_tag="ACHGMT_000419"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFN6834191.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(40479..40916)
                     /locus_tag="ACHGMT_000420"
     CDS             complement(40479..40916)
                     /locus_tag="ACHGMT_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834192.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(41133..42044)
                     /locus_tag="ACHGMT_000421"
     CDS             complement(41133..42044)
                     /locus_tag="ACHGMT_000421"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFN6834193.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(42501..42929)
                     /locus_tag="ACHGMT_000422"
     CDS             complement(42501..42929)
                     /locus_tag="ACHGMT_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834194.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(42957..43595)
                     /locus_tag="ACHGMT_000423"
     CDS             complement(42957..43595)
                     /locus_tag="ACHGMT_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6834195.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(43599..45635)
                     /locus_tag="ACHGMT_000424"
     CDS             complement(43599..45635)
                     /locus_tag="ACHGMT_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFN6834196.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            45761..46426
                     /locus_tag="ACHGMT_000425"
     CDS             45761..46426
                     /locus_tag="ACHGMT_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6834197.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
BASE COUNT        13800 a         9818 c         7967 g        15911 t
ORIGIN      
        1 tgtgaattta aaagaaagag gtcatttatg gaagataaaa aattgaaaaa acaacttagc
       61 tccagacata tcaccatgtt agctttaggt ggagcaatcg gtgcaggtct atttaaagga
      121 agtggcgaag cgattgggat cgccggccct tctgtattga ttgctttttt gattggtggg
      181 ctgattttat atatcgtgat gaaaggtctg ggaaaaatcg ttttaagcgg tggcgacacg
      241 catcacggat tatctggttt gatacgccct tatctaggtg ctcattctgc tgattttact
      301 gattgggtct actggtctat gtggattatc aatatcatcg ctgaagctgt tgcagctgct
      361 tcctttttgc agttgtggtt cccgcacatt ccagcctggg tatttgtttt tatgttggct
      421 gttttaacta cgatcatcaa tctttattcg gtacggttat tcgcagaaac agaatattgg
      481 ctggcttttg ctaaaatcag tgtcattatc ttactgatca tttttgctac ttatcttgtc
      541 ggtcagcaaa tgttgggcac aggcgttttc cctacccttc agcaattgac tgaccatggt
      601 gggtttactc ctcatggtat gaagggaatc atcagttctt tgcttgtagt tatctattct
      661 tacggcggtt ctgaactgat tgctatcacg gtaagcgaag cagatgatcc taaacgtgcg
      721 attcctaaag caatcaaagg tgtcatggga agaatcattt ccttttacat cattcctttg
      781 tttttgcttt tgattctttt tccttggaat actttagcag gcacgaatgt cagtcctttt
      841 gtgatggtat ttgaaaaaat gaatatccca tttgcagcgg atatcgtcaa ttttgtgatt
      901 gtccttgctt tgttctcatc tatcaattcg ggtgtgtatg catcttcaag aattttgttc
      961 ttccgtttga aggatcgaaa aggttcctct agaaaactag ctgttttaaa caagcatcaa
     1021 gtaccgcaac gtgctgtttt attttgtacc agtgttcttt atcttggtgt agctctttct
     1081 tattttgtcg gtgacaaatt atttggctat ctggcaggtt ccctttcgta tacagtatta
     1141 ttgatctgga tcattatcag tgcggcatcc tttgtactgg cactaagaaa aggaacatta
     1201 tgggataaag gaatcagctt ttttgcatta gctgtcttag gattgatttt ccttggtatc
     1261 ctgttcacga actccattgg ggtaactgta ttgactggcc tgctttacct gttcatttat
     1321 ttcagttatc aaaaaaagaa tgatgcgttc gtattgacga atgaatgaaa tcgatagaaa
     1381 attttatttt atttatgtgg aatagatgga gctattgaag aaaggagttg tgacaaaagt
     1441 cttgttgcaa ctcctttttc cgaataaaca gtctcatgac ctcttggtaa acggtgttca
     1501 aaaaccctct gtgcaaaata attgattgtt ctccatgctc atgacatgat agaagaaagg
     1561 aatgaaaaaa aggagtttgt tttaaatgaa aaagtattgg aagtttttgt tgatgatcgg
     1621 tgtttcaacg gtagttatgt ttggtatgat gtacttgaat gtctatgcgc tcgatcatat
     1681 cttcttcagt cgtacacgag tattcatggc gttgatgatg ggtgcagtca tggcagtcat
     1741 catgctgttg ttcatgtgga aaatgtatga aaacaaaaag ataaatgtta tgatcttagc
     1801 cgctagtctt cttgtttttg ccggttcctt gtttatggta cgaagccaaa ctactgtcaa
     1861 cgacacagct tggatgaagg ccatgattcc tcatcattct atcgctatcc tggcaagtaa
     1921 acgagctgat ttaaaagatc ctgaagtaaa aaaattagcg gaagaaatca ttcaggcaca
     1981 agaagaagag atcgacaaaa tgaaaaaaat gatcaaagaa atggaatgac cattatccgt
     2041 ccaaataaaa aaagaggatg ttccagaagt tatcagcttc aagaaataag aagaagctac
     2101 ttctggaacg ccgtgaataa ggggttgtga aaaagctgcc ctgcatcaag taataagaac
     2161 agccaaacaa aaatagcttc tcctattttt cgtttggtta ggcttattac cgcagatgca
     2221 agcttttgaa caccgtttat taagaggttg taaaatcagc gttctgacct gagtattaga
     2281 gagacaaatg gaaaaatagt tcctcatatt ttgccatttt ttgagctaat accgagggtc
     2341 agctgattga acaccgttta tcgagaggtt gtgaaaaagt cgcttagttt cgcgtattaa
     2401 gaaaaatttt gtgaaatggc tttccacatt tttgtataaa tttggcttaa taccgagaaa
     2461 ctggcttttg aataccgttt attcgtaaaa gggattgtga caaaagtctt gtcacaatcc
     2521 ctttttatgt ttttattgtt ctaattgcgc tgtcttgctt actgcttctt gcggtttcac
     2581 aaattttttt gagattcccc aacctgtgaa ggcactgacc atcgaaaaca caggagaaag
     2641 caaactgaaa aatacgaatg gcaagtaagt gagtgtagga ataccaaatg tacttgctac
     2701 gaaacttccg gcaattcccc aaggcactaa gtagttgatt actgttccac catcttctag
     2761 cgttctacct aatacagttg gatcaaatcc tgctttttca taagtttctt taaatgcatt
     2821 accaggcaga ataacagata ggaattgttc accaataaaa atatttactc cgatacttgt
     2881 catcaatgtt gtaaaaatca aatttggtgt gttcgtcatc tttcttgata ctcgttccat
     2941 cacggttgag atcaagccaa attcgatcaa caatccacct aatgacaaag ttagaataat
     3001 caatgacaca gtaggcatca tactttcgat tcctccgcgg ctaagcagtg aatcgatttg
     3061 ttggtttcca gtatttgaaa caaaccctga ttcgatgacc ccagccaact tcgttaagga
     3121 agtttgtggg ttttgaataa aaatcattcc tactgctaca gcgatattca acaaaatcgt
     3181 taaaattgcc ggcacctttt tccatgcaca cagtaacatt aaaaataaag gaatcagtga
     3241 ccagattgaa atagtaaagt ttgcttctag aacatcagtc acttcacgaa tagatgaaag
     3301 acttgcggct ttacttgtgt gtcctaaaat catgaataaa aagaaagaaa caacaaatgc
     3361 cgggacagtt gaccacatca tatgcttgat gtgtttgaac aaatcggcat caacaatagc
     3421 tgctgctaga tttgttgatt cggataatgg agacatctta tctccaaaaa cggcacctga
     3481 aacaatagct ccaacaacca gcgctggatt taagcctagt gttgtcccaa taccgaaaaa
     3541 tgcaatccca atcgttgaca taacagtaaa ggcactccct acagcgctac ctacgattgc
     3601 acacacaaca aatacagaag ggacaaacca ctggacactg atcaatttga acccaatgac
     3661 cattagagtt ggaatgatcc ctgcttgtat ccatacagca atcaatgaac cgactaaaat
     3721 aaaaataaag attgggatga ttcctggttt gatgccattg atgattcctt cgtgcagcca
     3781 atctgtcggg tatcttttta tcactgcata caacatgacc attccgatcg ctgctaagat
     3841 cgtgatatta ggtgagacct ttaatccgac aacgccaatc gcaatgccta aaacaataac
     3901 cattaataca agtgttgctt ctaaaaaact tggtgttcct ttttttccca tgataatctc
     3961 tcctttttta gttgttctta tgttctaaaa aagttaagat atcggattac tccgccgcaa
     4021 ttcatccgcg attcctcctt tataaaaata aaaagacctg ttttaaacta aggacgaatg
     4081 actccgcggt accaccttaa ttaaaaacag atctgttttt cactttcaaa cttttattca
     4141 aatgtatgga ctttccatgt aattcgtcaa ttaggcttga tcggttcaca gcgaccaccg
     4201 actttctgaa caaaacacct aaccgctact gcgtgaaaag ttgacttgta cgaagtttac
     4261 cttccataag atcatttgtc aatgaaaaag aaaatatttt ttcatttttc tgaaaattat
     4321 ttttataact ttcacacaca agtttatttt ttactagaaa gtagcgccct cttgtttttt
     4381 tcttgctact atttcttgat gttcttgctt gattctttct aatagctttg gctctttcaa
     4441 cagatcaaga gctgtattgg cgagtagttc tgctgcaatc gcaatagaag caaggccttt
     4501 gtcactttta gcagcagctt tgaattcttc tgtgtgtccg gcaatatatg tctcagagat
     4561 tgctaccgta ggctggattg ttggtatgac ttggctgaca tttcctacat cagaagaacc
     4621 gagacttgtt ctttgttccg tttctatttc ttcatctgga acacctgctt cttttgcatg
     4681 ctggaagaat aagtcatcaa aagaaggagt aacgatgaca tcatcaactg cattttggaa
     4741 caatccaaac tcaaaagtcg tgcccgtact tatcgcagca cctcgtacga tattttccac
     4801 tttttcatat actttatcta atgtttgccg atttcctgca cgtaaataga aacgtccagc
     4861 agcgtaatct ggaatgacgt tggcagctac tcctccgtct gtaatgatcc catggatatt
     4921 gacatctttt ggcaattgca agcgtagagc gttgattcca ttaaacactt ggatcaatgc
     4981 ttccaaagca tttactcctt tttcaggtgc tgctgctgca tgggaagcga caccgtaaaa
     5041 tttgatgtcg actggatcat tcgctaaaga ttctgtcgtt ttcccataac gaaaagctgg
     5101 atggacgcat aacgctacat cgacatcctc aaaaaaacct tctcgaacaa agctgccttt
     5161 agctgatccg ttctcgcccc cttcttcccc tggggtgcca tatacacgga tttctccacc
     5221 agtttcatca atgaattgct tcactatgct ggcggctaat acggagtaag taccaaacaa
     5281 attatgcccg catgcatgac caatacctgg taaagcatcg aattctgcca aaaaggcaat
     5341 tgtgggaccg ggtttattgg aaatataacg tgcatcaaag gctgttcggt gtccagctac
     5401 ttctttttct acccaaaacc tttctttttc taattgttta atcaacgctg ctgaggaata
     5461 aaactcatag ttacttactt caggatgttc gtggatatct agagcaagtg cttgatattc
     5521 tggtaaacga tattggatta gatccttcaa ttggtttgtt gagattgttg ttgtcataaa
     5581 aaattcctcc tatgctagtc atttttctta ttttctcccg aatgtttccc atgcaggcac
     5641 gcttgatcct tttgatgttt tctcgattac tttgactgtg tcttctgttt gataagcgtc
     5701 tactattttt tgatacgttt tattgttttc atcttctttt cttgcaacga tgatattcac
     5761 ataaggacgt gcatggtcat ccactggttc taagaagatg gcatcagaag caggattaaa
     5821 tccagcatct acagctacac cgctattgat cactgaaatg tctacatctg acaaagcacg
     5881 tgctgtttgg gaagcatcta attcagaaat gtctagattt tttctattct cggtaatatc
     5941 agaaacagtg ggcgcttgtc cttttgcctt atccactttg atcaatccag cagtttgcaa
     6001 taacagaaga gctctgccgc catttgtaac atcattaggg attgctactt ttgctccgtc
     6061 tttgatatca gctacatcct tcaaagtgtt agaataaatt cccagcggtg cattgacagt
     6121 atttccgatc gatactaaat cagttccgtg ttctttattg taattgtcta aaaagaattg
     6181 atgttggaaa gaattcaaat caatcgatcc atctgctaaa gccacatttg gttgattgta
     6241 atctgtaaat ttcacgtatt ccaaattaat gttctccttt ttcaaacggt ctttcacgtt
     6301 gtcccataca tctgtatctt ctccaataat ccctagtttt acgatagttt cttttttgtc
     6361 agttgtatct gcttttcctt ggctacaccc tgccaaccaa ataattgcta caaatgtaat
     6421 gactacaaaa cgaataattt ttttcatttt ttctcctcca ctttcaatgt gtaatttttt
     6481 taatcagttg attactgata aattgactta aaaaaactaa gattagaatc aataatgtag
     6541 ccacaatcgt tacatctgtt tggaatcgat tataccctct cgatatagca agattcccta
     6601 atccgcctgc acctactgct ccagccatgg tagtcagtcc gatcacgttg atgatagtca
     6661 acgccgagac ccgaatgata ccaggcaaac cttctaacaa atacacacgt aaaatgatac
     6721 cgattggact ggttcccatc gcttctgccg cttcgatcac cccaggatca acttctagca
     6781 gtgcgttctc gatttgacga gcaaaaaacg gaacaactcc aaaaatcaat ggcactaaag
     6841 ctgctttcgc accaatcgtc gttccaacta agaaacgagt agtcaatgct aaaagagaaa
     6901 gcaagataat gaaagggatg gaacgaatga tattgatcag cttatcaagt atttcaaata
     6961 gcagacggtt agctagtaat ccattagggc gtgtcactac taacgtcaca cctaagatga
     7021 ttccaatcat cccgccaatg aatgctgtcc aaaaagacat atatagtgtt tgcttcgtcg
     7081 cttcgacaaa ttcatccgga atcaaataaa cattgggcaa atatgtttta aagaaatcag
     7141 tcattttatc ttcctccttc gatcactttg atacgactat ctaaatcgtt tttttgtaaa
     7201 atatttattt ttacaccaga tttgatcaaa taatccaatg cttttttccg ctgtttggtt
     7261 tctccagcta aagtaacgat caaactccct aacggcgttt cttgtaataa ctctacattg
     7321 ccatataaaa tattcgctgt tacttgatat ttgctgtaca gatgagcgat tagaggttcg
     7381 ttcgtctgat ttccaatata acttaattcc accagccatt tattggcgat ctgatcggca
     7441 aaagcagaat gggcaatgat cgtttctaat gcttggtcaa gatgagtagc tgttcgaata
     7501 aaatctttgg tcaattcttc ttcaggattg ctgaagatct ggatgctgct acctttttca
     7561 acgattcttc catcttccat aaccgctacc ttgttgcaga tttctttgac aacttgcatc
     7621 tcatgagtga tcaggacgat tgttaaacct aactggcgat tcaatttttt taacaatgca
     7681 aggatctgaa gggtcgtttt cggatcaagt gcactggttg cttcatcaca taataatacc
     7741 ttaggttcat tcgccaaagc tcgtgcgatt gctactcgtt gtttttgtcc ccccgacagt
     7801 tgtttaggat aagcagatgc tttttcttcc aaaccgacaa gttccagtag ttcattcact
     7861 ttttgacgac gttcctgttt cgattttcca gaatacttta aagaaaaatc aacgttgtca
     7921 aaaatagttc ggctttccat tagattgaaa tgctgaaaga tcataccgat ggcctttctt
     7981 tcttttcgca attctttggg agagagctga ctcagattcg tctgattgac aatgacttct
     8041 cctgctgttg gtttctgcag caaattcatc acacgaacta acgtactttt ccctgcacct
     8101 gagtaaccga cgattccata aacatcgcct ttttcaatcg ttaaatctac agaatctact
     8161 gcctgtatct ctttttcttt ttgtttgaaa catacagaaa catctcgcaa ttcaatcatt
     8221 aaaattcctc cttatctaaa aaaagcgtcc tcaagaaaat gaaaatgtca ttttcttgag
     8281 gacgcttttt tgcgtgttac cacctcatgt tcaagctgtg ttcacacaca actcctcttc
     8341 aagtacgatc atactctagc aagatatcgg tcgcagccgt catcgcctta catctatgct
     8401 cgggatgaat tgctcgatgg ccattttcca atagtcgttc cttgcttctt ctcagctacc
     8461 gaagctctct gtcaaaggtt cttctatcgt acttaccatt tcaatgcaaa taatattcaa
     8521 aaaaaaagtc cttttactaa cataaactat gtcagtaaaa ggacgacgta tcgcggtacc
     8581 accttttttc ggaagtttcc ttccctcaag cccaacatct caaaaagaga gattggcgtt
     8641 cgttttaacg gtcgaacagt tccgaatttg cctacttatc gacaaattat tctccaaggc
     8701 cattttcagc ttttcttact tgactcttct caccagccag agcctctctg caaagtttcc
     8761 aaacttactt tccttttcaa cgaaatcgat attttgttgt tgggattaat ctacatgagg
     8821 aaaactgaaa agtcaagtac gaaattcaaa ttattttttc tatatttatt tttctgtttt
     8881 tttatttaac atttcaataa cagttcgaag atcttttcct tccgcaagaa gtttcaattt
     8941 ttccttttct tcgacggtat aatttcggac aaaactggaa tcaggtatca aaacaacgcc
     9001 taatcctgct cgtgaagcag ctgttgctcc tactaaagaa tcttccactg caaaggctga
     9061 actagcttct aatcctgttc gttccaaagc tgtcagataa ggaatcggtg aaggcttgtt
     9121 ctccgacact tcgtctccga atgtaaacgt gtccaagtaa tcagctaatt taaagtgatc
     9181 caagatatct gttgctcttt tcgtaacagt ggtcgtagca atgcctagtt tcatatgacg
     9241 ttcttttaag tctgccaata tttcttttgc atatggtttt aagtggatgg ctccgcttgt
     9301 cagctgttga taaaaatatt cttcacgctt cgctcggact tcctgtactt tttcagggct
     9361 acctgctgct ttcactaaaa tatcaaaact ttgtttccag ttttttccgc cccaagcgtc
     9421 aacgatttct tttgggatat ggatatcaaa ttgctgaaat ccccaaagcc agccatcacg
     9481 atacgttttt tctgtgtcaa ctaacaaacc atctaaatca aatattgcac ctttcatcta
     9541 tacattaatc ctttccaaaa caatacacat agtttagcac gactggtcca aaaagaacag
     9601 aagcaaagaa ccagccttct cgtttataag agttcttgga atcgacggat atagattttc
     9661 ttcacagctg tcacaagcgc cagataagcg atgactgtag caaataacca taaccagtaa
     9721 ttgccaggaa gtggcatcaa gcctaattca gcaccaaaac ttgtaaaagg aaggacagaa
     9781 ccgacaataa ttcctaatgt agtgataaca aacatggcaa atgttgcgtt gctttggata
     9841 aatggtactt tcggtgtacg cagtgcatgg agcactaagg tttgtgtcca tagtgattcg
     9901 acaaaccatc ctgcatggaa taatgcgatg aaaatcactc gctgtgaact atccaatgta
     9961 tggaaacttc cgcccactac agcaggacaa ataacaaaat acatcaacaa gtaagtagta
    10021 atatcaaata tcgaacttgt tggaccaaag taaaccatga attccccaat actagatgca
    10081 ttccattttt ttggctcgtc caaatattct ttatccatcc gatcccatgg caaggaaatg
    10141 caggaaacat catagatcag attcaaaaac aaaagttgta aaggcagcat tggtaaaaat
    10201 ggcaggaacg tacttgcaat cagcacggaa aacatattgc caaaattaga acttgctgta
    10261 gccttgatat atttcatgat attcccaaaa gtttctcgtc cagaaagaag gcctctttcc
    10321 aaaatcgtca aatctttttc taaaaggatc acgtctgcag attctttcgc aatatccaca
    10381 gctgtatcta cagaaatacc gatatcagcc actttcattg caggagcgtc attgatacca
    10441 tctcctaaaa atccgacagt atgaccattt ttacgtaaag ctgtcgtcaa acgtgctttt
    10501 tgctgaggat tcaattttac gaatacttca tggtcttcag ctacttgtgc taattcatga
    10561 tcactcattt ccgagatttt ctcaccggta accaattcat tgatctctag acctacttga
    10621 cggcacactg aacgtgtaac taattcgttg tccccagtca gaacttttac agctacacca
    10681 tgttctttca gagctttcaa cgcttgtttc gttgtttctt ttggtggatc aaggaaagca
    10741 agataaccaa tcaaaaccat gtctgactca tctttgactg aaaattctcc aacgacgctt
    10801 ggattggtct tttgtgcgac accaatcact cgtaagccat cttcattaag tgactgtact
    10861 tttgcaagta cggcttcttt tcgttctttt gttaatggtg taacagtacc gccaagatca
    10921 ataaatttag aaatgttcag catttcttca atcgcgcctt ttgtgatcat ttgtgtttta
    10981 ccagctggat cagcaaccac aacactcata cgacgacgtt caaaatcaaa aggaatttca
    11041 tccacttttt gataattgat cttatctgta tctaaagttt ctgttgcttc atcaatgata
    11101 gccacatcta acaaattttt cagccctgtt tgatagtagc tatttagata cgcatggcgc
    11161 aaaacacgat catcttcatt tccttcacaa tccatatgat attcaagaat gattttatct
    11221 tgtgtcaatg tccccgtttt atccgtacat aacacatcga ttgcaccaaa gttctggatg
    11281 gcattcaaat ttttgatcac tgttcctttt ttggccatca cacttgctcc tttgaccaaa
    11341 ttcgtcgtaa cgatcattgg aagcatttca ggtgttagac caactgctac agatagtcca
    11401 aaaagaaaag cttctagcca atcccctttt gtaagcccat tgatgacgat aactgctgga
    11461 gccatcaatg acataaattt gatcagtagc atggatgttt tatggatgcc taattcaaag
    11521 cttgattgaa ttggtttttc agatagcgtt tctgctacat gaccaaatag tgtttcattt
    11581 ccaacagaaa caatgattcc ttccgcactt ccactgatga cattacttcc cataaagaca
    11641 agattttcgt aacttgtttc gctttgctga tcgtcttcac atactttcgc atgcttttct
    11701 actggatagc tttcccctgt caatgctgct tgggaaatga ataaatcctt tgcggacaat
    11761 aaacgcatat ccgctggtat catatcccct gctgaaagcc gaacaagatc tccacagacg
    11821 atttcttcca taggaatctc tgtgtacttt ccattacgtt ttacagctgc cgttactttc
    11881 acaagtgatt tcagtttttc agccgcttga ttggaacgta cggactgaat caacgtcatt
    11941 gtcccgctga cgataaccat agtgaagata atggctacac cgaaaagatc tttatctcca
    12001 ggagctgcta atacatattc cgtgatgaat gaaataattc ctaatgcgat caacactaat
    12061 gtaaatggcg tgatgtatgc tttcaatatt tctacaatga atggtgtttt tttgccatat
    12121 gaaatcgcat tttcaccgta attttctctt gaacgttcga tttgttcttc tgtcaatcca
    12181 tttgctgatg catggaatag ttcaaataat tcttgtttcg tttttccagc aaaagctgca
    12241 tagatttttt gtgtatcttt ctttttttcc atctcaatct ctcctttgtg tccaaaagac
    12301 agctgttctc gctgcttttg gtctttagct attcatcgga tcgatagaga aaaaactgaa
    12361 aggggctgag aacagataga taatagccct ggcctttcct ctagatcctt gtttttcgaa
    12421 tgactgtcat ccatttgttc tcatctcctt tactaagtaa ttttaattca tgacttccaa
    12481 tcatttttcg aatagttatt tttccaatta gaccacctcc tttttatttt tcaccgatgg
    12541 ggatttgcgg caacaaaaaa acctctcgcc aaatactcag cgaaaggttt gaaaaagcat
    12601 attaaaaaaa gcttctcaga tctcatcgtt gagttttgac actgtacaac gtaaaaagca
    12661 gatacctgct tttagctacc ttatgaaaag ccttatctcg acaattcctg ttctacccat
    12721 tggcgtctct cgacgttttt gggcagtagc ctatgtttgt ataggagcct cacctaacaa
    12781 atcttattcg gttttccttc gttaatataa caactaaaaa agaggatgtc aaatcaattt
    12841 tccatgtttt tcatactttt tttaaaatac acacgttata ttcctgtctc acgcggtttt
    12901 atttgtctca ttttccacct cccgctataa tagaaatata gtaggaaacg aggtgctgtt
    12961 atggaaagaa cacttgttat tatcaagcca gacggcgtga gacgccattt agtcggaagc
    13021 atcatccaac gttttgaagc aaagggactg gccattgctg aaatgaagtt tgaaactatg
    13081 actcctgaat tagcaaaaga acattatcag cacttgactg agcgcagttt ttttgatgaa
    13141 ctgatcgatt acatgacttc cggtcctgtc gtttatcttg tgctggtcgg cgaggaagtg
    13201 atcgatatcg tccgtaagat ggttggagca acaaaagctg ccgatgctgt tccaggtacg
    13261 atccgaggag actacgcgct gccggggact gaaaacatca ttcacgcttc tgattcccgt
    13321 gatgctgcgg taaaagagat cgcccgcttt ttcccgccat tcgatataaa caaaccgtct
    13381 gaaaaagcgg cgaagtgata caaattctaa actgctggtc tagttgctgg gttcagcaat
    13441 ggccagcagt tttttctcta aggacctccg ttgattcttt cctaacatat attcgagcac
    13501 tgtgaagaat tcttggtagt ctttcgtctt ttcttcccct aaagcagcag aaatcaatct
    13561 ttgataataa tctgccatca atgtttcata gatcactgta cttctttctt tatgtgataa
    13621 gctccgtttc atatcttcat tcacactacg tgcctttttc aaccgaccat tttctatata
    13681 aaacgaagtc aggttcatta aaaaagtaaa gagatactgc tcatgattgt tcaactgtgc
    13741 taatcgtcgc attctttttt tgattttcaa ataattgttc tcaatgaacc gatcactgaa
    13801 tatccaagga aaatcagcta gaatagatag ttcaaattga ccatagtctg gtatctgcat
    13861 caagtattct tcaatgattc ggcattcata acgtaaaaat tcaaattctg tctcagatac
    13921 ttctgttttt tcgaccatgt gaagattcaa aaccaaataa tacagatctt ctgttttctt
    13981 aaaatgccta cggttattgc tgatctcttt ataaggatca aagctgtctt cttgtacccg
    14041 atctagcatt cgtctttttt tctttaactt tattggttca tccagctggt ttcgcatcca
    14101 ttcatattct gcaagtgtca tgttcattcg ctctaaaaat gatctcaatg ttacaaatga
    14161 catttttttc actgaatttg tctcaatccc gactagagaa gtcttaaaac agattccttc
    14221 cgctaattgg gactgtgtca acttcttgtt gactcgaagt ttcttgatca attcaccatc
    14281 atacataata gttctccttt ttaatttaaa tttttccaca ttcaataaat agatacgtta
    14341 aaaaggaaaa atacacgaaa aaaataaaaa aatgtttttt tcataaaaaa taccaacctt
    14401 caagcattag aatgttgtct aactactgaa ggttggtttg attgtttatt gattattttt
    14461 ttgctgttca acgtctgttc ttgttactgg atataagttg gttgttgttt tattaccatt
    14521 ttttgaaaag acacttgtcg aatgttcgcc tttttgcttt tcgattgctt cattacgttc
    14581 cgcttcattt ttatagtcat attttttcgg atcaatcgtc tcaaatccgt ttggcacata
    14641 aaaccgcaag agattttcct gattcagctt atctgaaaga cttaacgcag tcttgacttt
    14701 ttcctgatct ttggagattt cctcatcgat tcctgctgct ttcgtatcga tgatttctcc
    14761 tgtctgatta ttgtaagctt ttcctcctag gacggtgtat ttgggtgtca caaagttccc
    14821 atttcgaaaa gcaactactt gatcatgctg agaagataat aagtctgtac caaaatgaag
    14881 ataatcttta tcatcgattc ccaatagatg tagtaatgtc ggcaaaacat cgatttctcc
    14941 accgtattca tgattgactg tccctttagt ataaccaggc atatggatca tcaatggcac
    15001 tcgttgcatc tgtgcgttgt caaattcatc ccatgtatct ggatctttac caagagcgct
    15061 tgctaaatct ttattatctg tattcgagat cccaaaatga tccccgtaaa tcacaaagat
    15121 tgaattttgg taaataccag attttttcaa atacgtaaaa aattgttcca atgattgatc
    15181 cagataatgt gccgttcgaa cataattatc tactgtgcta ttgccagtat tcaaagatgg
    15241 aaagtcgaaa ttcttgtcat ccgtataata aggcgtgtgg ttggttacgg aaagaaattt
    15301 cgtgtaaaac ggctgttgta gatgctcaag atattgggca gattctctga ataaatcttt
    15361 atctaatatc ccataaccta acgtttcgtc ggattcatca aaataagatc gatcaaagaa
    15421 attgtcataa cctaagtttt tatatacatg atctctattc caaaaactag ctacattccc
    15481 atggaaaaca gcgcttgtat agccttgctt ttgtccaagg atcgctggtg cggcttgaaa
    15541 gacattgtct gacccaagtt ctgtgaataa tgagccttga ggtaaaccaa atgtgccagt
    15601 ttccaacata ttttcagcat cacttgtctt tccctgtcct acttcatgaa agaaattgtc
    15661 aaagctgatc gttgcttgat tctggaaaat actatttaaa aacggtgtga cttcttgtcc
    15721 gtctaccttc atattaatca aaaactgctg gaaactttct aaatggagaa cgatcacatt
    15781 tttcccttta gcgatcccaa atgtttcggg atttggctct gcataatgtt tctttgtata
    15841 atctaaaact tcatcaatcc cattactgct tgcatgcgcg cgaacactgc tagtcattcc
    15901 tgttttgatt ccgtcataaa tagtatacgc atcgattcct agatatttga caatatatga
    15961 acggtcaaat gttcgctgca gcaattgtgg acggttggct tcgctcagcg ctagatttcc
    16021 tgaaaaaata agccctgaaa gacaggtaac agcgatagcc atcggtttgc cgacagggtt
    16081 gcttttgata ggtattttct tgaccttcaa ccaaaccagt agcccaatga acacagcgat
    16141 atccagccag tagatgacat catggggctt catcaaagaa aaggagcttc ctgacaatcc
    16201 ttgcgacact ttcgaaaagc ctaatacaga tttgactgta atgaaatctg tgaattcccg
    16261 ataaaaaata atattcaagt acaacaaaag cgtatttaaa atatcaatga tcaaaagaat
    16321 agctaacgct ggctttggct ttttaatata caaagaaagg ccaaaaaaca ataaggtagt
    16381 cgcaattggg ttgatccaca agataaagta ctggatcgta ccttccacac ctaatgaaaa
    16441 atctgcatag taggcaatga tcgtcttcag ccagaaaaag aaaacagctg tcaaaacgat
    16501 tgctaaacga ttttggcaca attttttaag ttgtgtcacg tatatcactt ctctctattc
    16561 acattttttc ttattttgct cctgaaaaat gaaaatttcc taaaaaagtt gttatcttaa
    16621 gattttttta ttctttctaa agattattcc gtccactttt cacagcataa aaccgctgcg
    16681 atgtaagcga ttaaactaat gaagagataa aagtcttgtg gtgaccgctt cactccacgg
    16741 tattcaaaag ccagtttctc ggtattcgta caaaaaaaga ggctgtgaca aaattcttgt
    16801 cgcagctcct tatttcttga tgcaggacag ctttttcaca acttcttcct taaacggtgt
    16861 tctcctgcca actcctcggc cacaagtcac aatatttata aaacatgaga agctgttttt
    16921 tgaaattgtt ccttgtggct cagagttaaa cggcaggctc aagacctctt gatacacggt
    16981 gttcaatcag ctgaccctcg gtattagatt atgtcaggac gctaatttta caacctcttg
    17041 gtaaacggtg ttcaaaagct tgcatctgcg gtaataagcc caaccaaacg aaaaatataa
    17101 ggagctattt tttgaaattg ctccttgtgg ctcagagtta aacggcaggc tcaagacctc
    17161 ttgatacacg gtgttcaatc agctgacctt cggtattagc tcaaaaaacg gcaaaatatg
    17221 aggaactatt ttgccgtttg tttctctaat actcaggtca aaacgctgat ttcacaacct
    17281 ctgtttttca ttgtccatag gattgatatt gtccttgtga tgactcattc gtaataattg
    17341 aggcttgatt ttcttcactt cccgttatcg tttgtccact gccagaatct gaaggacttg
    17401 ttcctgtcga ctcgtttttt tgagaagaga cagttgtttt cttgacttgt tcatttgtct
    17461 ttttccattc cgctaaacga tcggcgacca cttttttgtc tactttttca gaataagcgg
    17521 tgatcacttt tccatttttc aaacgggcta ctttgtcgcc atcatgagtc aaaagcaaaa
    17581 cggtcaaatg cttgtcttta aattgatatt gtctgagaga aatcgcacta agtacgtcat
    17641 cgatattaat aaattgattt tcttcttttc cttttggcaa ataaatgtcc aattcgcctt
    17701 tttcgtaatt ggcagttatt ttatactccg cccaccaata agttgagaat tcttttttga
    17761 aattatctac tgattctttg cttgctacga ttttttctgt ttctttctga gcaagatctg
    17821 ctgtcatttt tcctgaaaat gctgcggata aaataagcag taagaaaaaa ccgaataacc
    17881 aaaagagaag aaacggtctt tttttcctca tgctttcctc cactcctatt taattttgac
    17941 tattctatta tatacaaaaa atgaaataga aagacaaaaa agttgaaaaa gatcgtaaat
    18001 aattgaactt ctttttcaca aaatggtaag aatggagaat ttcacgcatt tgctctcaac
    18061 tattctttct taagcctcaa atccgttttt atctgatacg tctttgaacc aatgacccga
    18121 ttttttgatt gttctttttc catcttgtgc taagtctact gaaataaagc catatcgatt
    18181 cttatatgca ttggtccacg accagttatc catacatgtc cacatatgat agcctaaaca
    18241 attggcacct tcttgaatgg cttgatggac atatttcaga tgagattgga caaattcgat
    18301 ccgataatca tcgtggatca tgccatcttc tttgataaat cgtgcttcgt tttctacacc
    18361 cataccgttt tcagaaataa agcagtcgat atttccataa tgatctcgta cattgatcaa
    18421 aatatcatag attccttgtt catagatctc ccatccgcga taaggattca ttttacgacc
    18481 aggcatttca taattgtcga aataatcctc aggcattgga ccgtttttag tatcgattgg
    18541 tgtttctttt gcttttaccc ggcgtggctg gtaataattg atacctaaaa gatctactgt
    18601 attttctttg ataaccgtca aatcctctgc atggatcaca ggcatgagat caagttcttt
    18661 tactatctca atcaattcac ttggaaactc gccttttaca gctggatcaa ggaatgaacg
    18721 attgaagaac gcatctgcta tttgcgctgc tttaacatct tcagggtttg attcgtctct
    18781 tggatagctt ggtgtcagat tcaagatgat accaataaca ccaccttgct ccatttcatg
    18841 gtaagatttg atggctaacg ctgaagccaa cgcttcatga tagccaactt ggatcgcttt
    18901 tttcatatct gactcttctg gataatgcca tcgatataaa taacctgctt cgactggaac
    18961 gattggttca ttatgagtaa accatttttt tactttcgaa ccaaacaatt caaaacaact
    19021 tttggcatat ttttcatagg cttcaaccgt ttctcggttc aaccatccgc ctttttcttg
    19081 cagatacatc ggcatatcga aatgatacag attcacaaag ggttcgatac cgttttcaat
    19141 caactcatca aaataacgat gatagaaacg aacggcttct tcgttgattg ttccagattc
    19201 atcaggtatc aaacggctcc attggataga tgttcgaaat gaattatgcc cagtctgttt
    19261 catcaactgt acatcctctt gatacttcgt ataaacttgc gaggtttttt caggtcctac
    19321 ttgatcaaag aacttttgcg gttctttttt gtaccaataa tcaaaaatgt tctctccttt
    19381 tccatcgcct gaaatccttc cttctgtttg tgggccactt gctgcagaac cccaccaaaa
    19441 atttgctgga aattgatatt ttttcatcaa agcacctctt tttctttttt agttcgtttt
    19501 agttgattcc atgtatgata ttttccatca caatcacttt ttcattttct tcctcttctt
    19561 ttttgattcg ttttaacagc tgttctgctg ctcgttcccc gattttatct gttggtggcg
    19621 tgatcgtgat tttcggaagg atactcagat cgtttttatc taaataatca tagtgactga
    19681 acgcaatatc cttcccgatt ctgacacctt cttgattgaa acattcgatg gcaccaatcg
    19741 acatattgta attgctgaca aaaatagcag ttgccccttt ttctaataat gcttttgccc
    19801 cgtaatagcc gctttttcta gaatagtctc ctgtaaagac ctctaattgg atcgatggat
    19861 cattttggac cgcttctttg gctcctgcta aacgttctct tgctacgtag tctgtttgag
    19921 tggcagcaat aaatccgatt tttttatgtc cttgttcacc atgatgagcg atcacttttg
    19981 ctacactttc tcgatcattc actaatattg tatcaatacc tgctggtcct tctggacaat
    20041 tcaatgcgat aactggaata ctcatttttg tgagcatttc aaatccagac cactcttctt
    20101 cggataagaa gacaatcagc ccatcgatca catgcttcat caggtattca attttttgtt
    20161 caattttttc tggctgatcg gcaaacccgc ttaataataa gctataccca ttttcttcag
    20221 ctactctttc gatccctgag acaacttccg acccaaaacg gctggaaatc ccgtttatca
    20281 aaagtccaat agagaagctt cgattattct tcaacccttt agcaatgatg ttttctttat
    20341 agtccaacgc tgaaatcgct tctgctattt ttttcatgtt cttttccttc agttgctgac
    20401 cattcaagta acgggaaact gttcccactg aaacaccagc catcttagcc acatcacgga
    20461 tcgatgccat acattctcac tcctgccagt tacatttgat ttctctttct tgttgttcga
    20521 ttatgatgac cgcattcttt gcatagtgtt gcttgtcatc aatatccata taactaaagt
    20581 cttcagtaga gcaacttcct ctcactatta attatactta aacaagtatt tgaaccggtt
    20641 catttttaat atagcatttt tttaaaagat tgcaacactt ttttaaaacg ctttctgaaa
    20701 gtggcggatt ttttgccttt ttaaaatttt attctaaaat ttactctatt tgtgggtgtt
    20761 ttgtgcgttt ttctcttcca tccgtttgaa gttttacatt ccaatggtat aatttcttta
    20821 tcctatcaaa tgggggaagt attttgaaga aattaatcgt actatttatt ttgtctgcgg
    20881 gacttttgac attgactggc tgtgatttgt ttggcggctc tgaccaatcc tcggacgaaa
    20941 caaccgattc ttctgtctta aaagcggaaa aagcagatac caccaacagt gtatatacgc
    21001 taaaagtacg taaagatatg gatttatccg cgggacagtt gacatttaca gataaaaaaa
    21061 tggaatggac acgcacgtat cagacacaag gaactgattc ttctgaatca tctgaagaaa
    21121 aaacagtgct gacagatatc aagatcacta ccaaagatga cgaatatatt atttcaggaa
    21181 aagaaaataa aaaagaaaca acagtcatat ttacaaaaat cggtaattac cgtttgaagg
    21241 atgaggatgg caacatctac agtctatagt gccgtctgtt ggatcttctt ttgtttctcc
    21301 ccttcttttc tgctatagta aagaagcaag atgattgtaa aggagacgaa atggatgatc
    21361 gatagtcctg attattgtac atgtaataac aacaaaaata tccatacaga atttaatgac
    21421 caaggaagct tgagcatatg cgacaactgt cacaaactag tcaaagattc atttaaaaca
    21481 tttgaatctt atcctgttca aaattatgta aaaagtagaa gaagataaac aaatccaagc
    21541 aaaactcact atggctgatt tttatgaact tcaaactcag cttcttcctt tgatcaagct
    21601 tcatcagtaa aacaaacagt ggatcatttc agtttacaaa tcaaagagta aagatttatt
    21661 tatttcaaat atttgaatat aaaataacca aacgatagaa aggcttattc tacatcacgt
    21721 agtaattttt ctttcccgtc gtttggtttt ttatttttag cctaattctt gaccttttgc
    21781 atgctggtta tccgtaaaat attttccagc aggctttcct tccaattcag ctaatttttc
    21841 ttggatctga cggatcacct tttttgcatc ttctggattt tctacaaaat cgaatttatc
    21901 cccatccact tgtattttag gacatacatc aaaatcttca taccattgat tatagcgggt
    21961 gttcaacgtt tcataatagg tatataacgt ctcatcgctt tctagctgtt catagtcacg
    22021 cccacgtttt ttgatacgtt ctagcatcgt gtccaaagaa actttgatat ggaccatcaa
    22081 atcaggccgt ttttttggag caacatcatc taactcgttt agcatcgtat ctaataagct
    22141 atcgtactgc tgtacttcga tatccgttac ccgacctaaa tcagcgttta gatggaacaa
    22201 caggctatct tcatagatgg atcggtctag cacattgtca tcgttcacta aagcattttt
    22261 cattgctaaa aatcgcttgt tcaaaaagaa gatttgcaat aaaaaagcgt actgctcagg
    22321 atttgaataa aagagcggca atacttcgtt atcttcgaca ttttcataaa atggtttaga
    22381 atcaatctct tttgcaagca tatccgtcaa actgctctta cctgcaccaa tcgttcctga
    22441 aatcaaaatc actgacatac aacccttcct cttctataag tactcgccta cgtaagtttc
    22501 aacaggcaat gtttcaaaat catattcacg tacagcttgg tctgatttta aatacgtatg
    22561 gagactttgt gtaatcatca agttcgtagg taccgtctct tcttgtacaa tgatcatcgg
    22621 ttttttcaac atagttgcca cacctaattc gtaagccgtt cctggatcga ttgtttgatg
    22681 ctcaaagtca aggatagcaa caacaaattc tgcatttgtc agttcttcca tatctttttc
    22741 gtatacttcc tgcgcccatc cagcgctgaa tagttcataa ttgctttctt ggtgatgacg
    22801 tggagagtag aaacttgtga ctgttttgtt ttcctccaac gctttttcga tacgacttac
    22861 tcgatcgatt tgctcctctg aaaaaaatgg tcctgctaaa tatatttgac tcattatttt
    22921 gcctccttca ataataactg atttttaata gatactgctc gtaattcatt gaccagttcc
    22981 aatggattgt tggaaaagat tacttgatcc aaccattttt tatccataaa atcctctttt
    23041 gcaaaagttt ctaataattg ttgcaagccg ctgtaataac cgccaatatt caatattccg
    23101 attggcttgt cgggcgtcag accgattgct atccaagaaa gaagttgtga gaattcctcc
    23161 aatgtaccta gacctccagg gaaaacgagg acagcatctc ccttttcaaa taataatacc
    23221 tttctttcat cgatcgtttc tgtaggaata aatgtagtaa catcttttcc aggcaattca
    23281 gtttcaaaaa gcccattcgg atagatacct gtgacttttc cgttattttc cagtactgtt
    23341 ttactaatca ctcccataat cccggagtat gaaccaccgt aaaccagttc gatttcttct
    23401 tgtgccatgt attttcctaa cgtttgcgca ataaatttgt aactttcttt attaccaaaa
    23461 cgggaaccgc aaaaaacagt aagtttcata aaaaaacact ccctaactca aaagtctatc
    23521 ttcattctac acgtgttttt ttaaaataaa agactagatt ttgtagtttt ttagtggtat
    23581 ataggataga cgccactata tatagtattt agaaaaaaat catttttcag ccattttata
    23641 gataaaacaa aaataaataa cgtgttttca tcgatatgaa atgacttact tatagaagat
    23701 actgggattt cgtcctatat gccaataacg tgtattattt taaacaacaa atacttgttt
    23761 cttttattaa cgaaaaaatc attttgttct cgtgttatgt ttcactgata aatgatgttt
    23821 ctctaaataa aaaagccgac gaactaaacg atcaaaattt gatcattttt gttcatcagc
    23881 ttctcctaaa aatctttttg ttaacgataa atagctggat ttgtaaaaat atcacttgaa
    23941 tcgtcactgc tggaagaaaa ttcagtgata accgcacctg ttgcgcctgc ctggaactag
    24001 tgtttgatat tgggctcgaa cgtgtactga tctctagcat gaagaataat ttcttttttt
    24061 actgtataat aactttcttt cctttttgga atagtacagg aaagtttttt tgtttccttt
    24121 ccttcgacat aaggtatacc agaccctttc gacaacgaaa ggttctcatt tttccttcat
    24181 cgtcatttca aggagggtgc cgatgctctg gacagtttcg atcgctccta aaccaaaatc
    24241 tgtatattcg attctttcta actctgattc tttcatacca ttcctgtctc aaggtacatc
    24301 tattgtactt ttttcttgta atccattctg cctctcctaa ctaattgtca aatcatccca
    24361 agatatgtct tcttcctctt tttcttcttg gatgatttct gcattcggat ctactggttt
    24421 tttcaataat aaaatggtta ttcctgtgac aaaagaacca gctaacaatg caccaatgaa
    24481 tgcccatggt ctggtcatcg tagcgactgc aaacattccc gatgccggaa tacggctatc
    24541 tgcgccccaa aacatgctta atccgccacc tactgtccca ccgactcccg ttgctatgac
    24601 attgcgtgcc aagtcgttca atacgatagg gaaacatccc tctgtgatct gacagatccc
    24661 cataggaaaa gcggtcttca atgtatcgat ttcttgacga ttgagtatat ttttacgaat
    24721 gattttccct aagaaataag cgatcgtcaa gccgaatggg gttaccatcg aagctaaaat
    24781 caatacagtg attggttcat agattccttc tgaaaatcgt gccacacccc cttatccaga
    24841 ataaatggta atccagaaac agccgtgtat ctgcggtaat aagcctaacc aaacgaaaaa
    24901 tatgagaagc tatttttgtt tggctgttct tattacttga tgcagggcag ctttttcaca
    24961 acctcttttt ttatttatgt ttcataatca atatggtaag gatgcagctc cgtatggtag
    25021 accgcctccg aatattcaat catttcccca atttgcgtag acgatttccg tatccgtttg
    25081 atgccgatct gtgcgtctgt tttcagaatt ccttgttctt cttctgttaa aaatactgcc
    25141 gagaaatcat cagaaaatga gtcaatagta tatccttttt tcgtcagcaa acgatacaaa
    25201 gattctttgc caaattcttc tctgctgaat tgcatcattt cttgtggaag ataatgaaca
    25261 aaataaatat aaggctgatt gttcaaataa tataagcgct taaaacaaac agcttgctca
    25321 ccgaaaaagg aaaacaaagg atcttccttc tttatatgga ccaattgcaa atccagcgtt
    25381 tctttccgga cttcatatcg ggagttctcc aagatttgcg taaagcttgc cgctttggat
    25441 aatcgattgt aaggacgatt gcttagaacc gtcgtcccac gaccactttt ctttgcgaca
    25501 tattcatctg atgccagcag ttcgattgcc tttcggacag tgattttgct gacttgaaac
    25561 agttcttcta attcactttc tgtcggcaac atcgaaccga ctggatattt tcctgagaaa
    25621 atatcttctt taattttatc tgcgacttcc aagtatagta cacgtgtcct tttcataacg
    25681 tcaccctttg ctttactatt actaaaagaa tagcacataa aaaataaaaa aagaacacaa
    25741 atcacatgtg agaagtgtgt tctttttcat tcagctgctt taattacaag tttacgccat
    25801 ttgaggcaat tacttcttta taccaatcaa aagatttttt ctttgatcga ttcagtgtcc
    25861 catttccttc atcgtctaga tccacataga taaagccgta acgtttactc atttctcctg
    25921 ttgatgcact gacaagatcg atacatcccc aaggcgtata gcccatcaaa tctacaccgt
    25981 ctgctactgc attagccatc gcttcgatat gacgacgtaa ataatcgatc cgataatcat
    26041 caataatcgt gccatcctct tccatatgat caatggctcc aagaccgttt tcaacaatga
    26101 ataatggttt ctggtaacga ttgtataatt cattcaatgc gattctcagc ccttctggat
    26161 cgatctgcca gccccattca cttgcctcaa gaaacggatt ttttactcct cccagcaagt
    26221 tcccttctga agttgctgct tgtggatcag tctcattgat tgccgtagac atataataac
    26281 taaatccgat gtagtccact ggatattctt ttagcaactg gaagtctttt tccgtttgct
    26341 ctaatttttc cggatcgact ccatattttt tatgggtacg cgctgtgtat gcagggtatt
    26401 ctccacgtac ttggacatcc gtgcagaaaa aattgaattc ttggttttgg atcattgttg
    26461 ctgcctgatt gactggattg gcatcgatgc tgtaagttgt tgcatagata atcatgcagc
    26521 cgatttggat atccgaacgc aattcgtggc cgatttttac ggctttgcta cttgcaacga
    26581 attgattatg ccatgcttgg aaaatatttt ggtactctcc tgctccattg ctttttacta
    26641 atccttgact taaagctgga aagtgaaagg ccgaattgat ttcattgaaa gtcatccaat
    26701 atttgacttt actgctgtat ctctctaata ctgtctgagc aaatcgttca tagaagtcga
    26761 tcagttttcg atttttccag ccaccgtatt cttttgccaa atgaagaggc atttcgtaat
    26821 gagaaatcgt gatcacaggt tcaatatcat attttaagca ctcatcaatc agctgatcat
    26881 aaaattctaa tcccgcttcg ttaggagtac cttcgtctcc atttggaaaa attcgtgtcc
    26941 aggcaatcga aaaacgataa catttgaatc ccatttccgc aaacaaggca agatcttctt
    27001 tgaaacgatc gtaatgatcg attcctcgat gattcgggta aatatatttt tcttgatcaa
    27061 tcgtccagtc aaattcttca ctgccgataa tcgcaaagcg ctgcttacct ccaggcatag
    27121 catctgcaac ggatagtcct tttccgtctc gatcataagc cccttctaat tgatttggca
    27181 gcagttgctc caccccataa aaatttttct gaaaaacgtg attgcatcct gatttcctcc
    27241 ttattttaca attgccagaa catcttcgtt tgcaataatc tctttttggt ctaattcaag
    27301 tatatcggca aatgcatcag tattcgtaat gacaactgga gtgattacgc tatagccagc
    27361 tgctctaatt gcttcgatgt caaaacgaat caataaatct cctgctttga cgagatcccc
    27421 ttgtttgact aagatctcaa agccattccc atttaattct actgtatcca tcccgatatg
    27481 aatcaaaatc tccgctccgt cagtcgttgt gatccctaca gcatgtccag ttggaaaaat
    27541 agtcgtgatc tctccgtctg caggtgcaac tagttctcct acttctggat caatagcgat
    27601 accttttccc atcacgccag aagaaaaaac ttggtctggt acttcactca atggcagtac
    27661 tttacccgtt aaaggacttt ttaaggtatg ccgtgctgaa gtaatcggtt ctcctgcatt
    27721 cgcatgtttg ttttctaatt gattgtcttg tgtttgttcg tcaaatccta aaatcaatgt
    27781 acctacgaat gcaagaacaa aagcaattcc tgtagctatt actgctactg tcacatttga
    27841 ctctacacca tccaccgtat taatgaaagt cggaatcgtc aataagctga ccaaactgct
    27901 ggagaaagct tttactccgg aaaatccgat aatagctcca ccaatacctc cagaaataca
    27961 agcggcaatg aatggtttct tcaaaggtaa tgtcacacca tacacagttg gttcagtgat
    28021 tccaaataac gaagtcactg tagaagaaat ccctaatgct cgaagtttcg tatcttttgt
    28081 tcgcaatgct acagctaaag cagccccacc ttgtgccaag atggctggaa gaagcattgg
    28141 catcaataca tcaaacccat attgttcgat attcaaaaac atgatcggta cgaatcccca
    28201 atgcatgccg aacatcacga atacttgcca taaacttccc atgattagac ctgctacgat
    28261 tggactaaat ccataaatcg agttgaagag accgcctaaa gcattaccgg caaccgtccc
    28321 gattggtcct aaagcaagaa atgttaatgg aaccatcact actaaaacga caagcggaac
    28381 taaaatcatt tgcagaaatt gcggaatcac tttttttacg aatggctcta atttactttg
    28441 aacccatacg gctagaatga ttggtatgac agaagaagta taaccagatg gtgaaagaat
    28501 cactgggatc ccaaagaaac tgatagcttc tcccgcacca gcaactgctg tgatagctgg
    28561 atacacaagt gccatagcaa ttgctactgc taaaaactgg ttggctttga actttttagc
    28621 tgctgtaaaa gcaagcatga caggtaagaa ggtaaagaca ccatctgctg cagcaaacaa
    28681 tatttgataa gtaccgctat cagctgtcag ccagcccatc accgttgcca atgataagaa
    28741 tcctttcaat accccggcgg ctgccatcgc ccctagaaaa ggtgtaaata tactagagat
    28801 aatatcgatc agacgattca aaatattttt ggactcttct tttccatcct ccgaaactgc
    28861 aagaccttcc tcatccacga tcgattgata aacatctgct acattactgc caatcactac
    28921 ttgatactga ccgccacttt gtacgacttg gatcacgtcc gggtcttctt ttaacttttg
    28981 cgtatctgct aaagattcat cttttaatct gaatcgcaac cgcgtagcac aatgtactaa
    29041 acttttgaca ttcttttgtc ctcctaccgc ttcccataca cgacgaccaa ctgcttgatt
    29101 atccattgtt ttttccttct ttctttttta tttaaaaaat aaaaacccag attgaaaaaa
    29161 acagtcacaa acattcgtga acctattttt tcaatccagg ttttgcctgc catacagtaa
    29221 caatccttat gattggatgc tttttttctc acttactact ctttgaatat gaatcgttaa
    29281 ataaatctgc tctgcgatcc ccatttcctg tttttgcgtt tgtcgaagat actcattgat
    29341 ccgttcgaca caatgaaacg cttttggata ctttccttga actaatgcgt agagaaattc
    29401 atcttgctcg tcagatcctt cccctttgag ataacgctgg gcaaaaaact gcagatgagt
    29461 aatgatacgc tgataattca aagattcttc atcgaacacg atgccgaaga atttactgat
    29521 gatactcagt atatctctta ccattgtggt agcggtcatc gtcacttgca tgtcttcgtt
    29581 tccttgttgg ctattcacaa aatgaaaggc aataaaaccg gcttcatcct cagaaagttc
    29641 cacacccgtc ttttgtttca cgaacaaaag agcctctttc gctgcttgat attctttcgg
    29701 ataaaatttt tttgtttcaa ataataatgg attaggcagt tggatatttt cttttgctcg
    29761 tgtgatcgca taatgaagat gatcggtcaa tgtcagataa atattaggcg aaaattcaac
    29821 ttttagttta tcttcagcca gttcgactac ttgtttgacc cattcgatct cctctgccgg
    29881 aatatcagcc atcagctgct ggaattggtt tttagcaaaa gaatctctta aacaaattct
    29941 ttttggaccg cttctggcga aatctcgtcc ccaatctttt tttgaaaagc tagtcctttt
    30001 cccatacaaa cgacttcttc cccgaatcca tttttcgaaa tgacgacatt gttattcaaa
    30061 attttatcaa ttttcatttt gcttcgccct ctcaagaaaa ataaaaaaac ctagaattcg
    30121 cctactgttt cttagctgaa ttctggtttt gcctgccgta cagtaacaat ccatttctta
    30181 ttcaaaatca tcataccatc gaatttttat ttatgcaagc cttttcatat aaaaaataaa
    30241 aaaactggaa cacaagtcta tctagaaaac aaccaaatca gagatgcacc tctttgcgtt
    30301 tctctttttt tcagcaaatt ggtttttcct tatcgattca tatcctttgg ctgtttttaa
    30361 tctgtaacga cttatgtccc agtatcctaa aatgtttttg tctctagatt gaaagcagat
    30421 ctcatatcat ctgtcgcatc ttttatcgat gtaacacttt ccagttctcc ttgtacaact
    30481 agtcgcgtga cggcgtccat gtcgccacaa gtaattagtg tgacaatttc ttttccctcg
    30541 acttcattca gtaattgggt atctgtagga gcaacttttt ctttaaaaaa tgttttgtat
    30601 gtatacacat tttctaaatc tgtcaaatag atcttggcac ccatctctaa attttctagc
    30661 ggtgtaaaca gcaattcagg gttcgttgca cgatggcttg ctaaggcata atttcctttt
    30721 cccatttctt gatctggaga taatgttcct gctccgtaaa gtagtgacgt attatccaag
    30781 cctttgaaaa ccggtaaatt gattgaaact gaagggactg cgatactagc aatcactggt
    30841 agatctgttc ctctcatttg cgacctcatg accccttctg tcgtcattgg ttctaccgca
    30901 tcaaagtcaa aactgacatc tttatcgtta ttctttttca gatcttcttt tgttacattc
    30961 gctatagcat atttatctcc tgtctctctg acaaaataat ccttgatctg ttcattgaaa
    31021 atcaaagcta gtcctactaa taacaagagg aacaagagga tattgactag ccatcttttt
    31081 gcttttcctt tttttttcat tttctctccc tccagactca ttttactgtt tttcttttgc
    31141 tctttccaac agccgaatga cacatttcta caactctgtg tatttttttc gtacacctgc
    31201 gcttttttct acaggatatt tcaaattatt tttgatcaat ttttcttcaa tgatctcatc
    31261 tagatccagg tgaaggttgt ctgccatcat gtatgaatag atcaatacat ctgccagttc
    31321 ttctttgatt cgttctaatt gtgtttcttt tacttcttcc ggcgttttcc attgaaaaag
    31381 ttctaacaat tcagatgctt ccaaagaaat agagatagcc aaatcttttt cgttatgaaa
    31441 ttgtcgccaa ttacgttcat ctctaaactg gttgactttt tccatactat tcatttgctt
    31501 gccttcttcc aatcacttct tacttgatta atcataccat ttttttacat aaaaaataga
    31561 tgatgctttt aaaaaaactc ctgttttctt ttatttaaaa taaatgtcat agaaaatcaa
    31621 taatttaggt agccaccatg tgtatgcagt agcgtgtctt cttcccttga ggctttttgt
    31681 ttttcgatac gttcttcttc ccaaacagac aacgctacta gaatctctct taatgatttt
    31741 ccttcttcgg tgagcgagta ctctacttta ggcgggactt ggttataggc ttttcgatgg
    31801 atactttcat cttgttctaa ctctcttaac tgctgggtca gcattttttt tgtgatcgta
    31861 gggatttgct tgcttctcca cgcctaaaat aagcgatatt gctcttccct ttatctggtg
    31921 taagcaaagc agttagcatt ttgacgatcg tgggttccgc ttttcacttc ttatctactt
    31981 agtatcatga agtatacttt cgtactaata ctaagtagcg gaagtgtcaa ctactttgtt
    32041 taactacata gcaaaagaaa cagaagggtg acaacggtca tcacgcttct gtttcttttg
    32101 aatatgctgt gttgttgtct ctatctccgt gtagactttc ctcaaactta tcctttgaca
    32161 gtaagtcagt aggctgattt gctctttcta gatgccagat acatttctac aaaaaaatag
    32221 tgattctgtt ttgaaaattc gatttttagt tctgcatttt cgtaaacatc caacattttt
    32281 ttcgcactgt atagacctaa cccgccattt tttttagagg tttttcctct tttgaccatc
    32341 tccgatagcg tcaaattcgt tggatttgcc ttgttcgcta tactaaaacg tgatccctct
    32401 ttttcttcca gcagattgat cgtaatcgga ctctgatcgg ctacacgatg atcgaatgcg
    32461 ttagaaataa aaatagataa acagcgaata aaaacaacca gatcaatcgg gattgttttc
    32521 acttcgacag gaataactac ttggatcggg attttctccg attctgcttt ttctagaaat
    32581 tttaaaaaga cacccctgac agctggggat acaatatttg ttagctgttg tatttgcggt
    32641 ttttcaaaaa tagctgcaga atgatcttct aatatttcta aaaattccaa cgtttctttt
    32701 ttattttcct gaagagtaat ttttaatgaa aacagtagac ttttataatc atgtttgaat
    32761 tcctctagat gatggtaata ttcttcattt atttgataca gttgaagcga ttcctgatgt
    32821 tgtctttctt ttacttggaa actgatcaac ataatggaaa aaaacaaaga aaaaaaactg
    32881 atgactatcg acaaaagaga tagaagcaga taattagatg agcggaagtg aacacttccc
    32941 aataccaagt aaatcacgcc ccagcttaat aaaatacaaa tacctccaaa atagtgttca
    33001 gttggataag taaaaagcaa gttgagaaat ctcgtctgtt tgtctatact ttttactaat
    33061 agatagaaaa taaatgcgat aagtagtgca aaaaaacttg ctgttccaag atgtctcttt
    33121 cctaaataat ccatcagatc aaaaaccaga aaaaagctga gtccagtgat agcaaaaaaa
    33181 agattactga tcaaacagct gataaacagg tttttcgtag taaaataaaa aagaaacatc
    33241 aataataagc caactaggta aggaaagctc actttaggga taggaagtaa aagtgtcacc
    33301 accatcaaag ctatacaacc aaagctgttt tttactagca gttgtttcga aaagaataaa
    33361 tagataccta aatctaaaat caaagtaaag gtcaaataaa gtgtcactgt tgcgaccatc
    33421 ttattcctcc attgttttgt ttcaaaatgt acacatattt ttttaatagg agcctgcata
    33481 tgaaaaagta ttttatcata gaagataacc agtttcacag cgaatggatc atcagagaat
    33541 tggtcaatca tcatatcgag aatatcgtgc agctcacttc tatctctcaa atcaatcagt
    33601 tgatccagtt gggcactatt gataatcacg atgttttctt tatcgatatc gacttgaagc
    33661 aatcactcac aggtattgat gtcgccgaac aaatacgtaa aaaaaatgca catagtaata
    33721 ttgttttttt caccgcttat acgaattttg cagtagagat catcaatcgg aaaatccttc
    33781 cgattggata tatcagaaaa gacaggaatt tatctgaaca aattcgtcaa gtgctagaac
    33841 agatcaattg gaaagaacag cagatcgtaa atgtcgataa aatctttatt gatcaaaaag
    33901 gtggaagtct cgtcctgtat cctcgggaaa ttttatatat tgaaagtatc aaaggtttga
    33961 aaaaccaagt tatggtcaaa acaactacag aagataaatt agtgaatact tctttaaaac
    34021 aattgaagac agagttagaa aagtatgctt actttttact tttaaaatct tactgtatca
    34081 acttatcgca attgcagaaa attgattctg ctcattatgt gttagaattt ttcaacggtg
    34141 actccttact cgttggtcga aagatctttg aaaagacaaa ggaacgtttc catgacttcc
    34201 aaaaaactgc ttcgagttga tgaataagag ctgagacgta agcatagcag atttcaggga
    34261 gtgggccttg aatcggctcc actccctgaa atcgttgata tgattttacg cttcctcttc
    34321 tgtaggagca tctgttaacg accatgtgaa tgttgaaacg tagttgtcta cagcataatt
    34381 ggatacagga acaaataatt ccactgattg tctcccttgt tccacattct ctcctaatga
    34441 ataaatccat gtgccaatcc cttcatttgc tcctgcagtt actagatcta ctgcctgatc
    34501 tgctgggatt tcgattcctt cagcgccagt tccagaaacg gtagatggca ttccactgct
    34561 attagttgcg ctatttcgaa tcacggcgtt tttgaatttc acctttgaac cagttagttt
    34621 attcgtatca gatgttgtac caataaattc tgttcgtttg actgataatt tccaaccggc
    34681 ataatttccg cttttatccg ttacttgtac gaaattctgt acttgtgttt gtgctccagt
    34741 actgtccgtt ccgctttgta atgcagcata tactgttttt gctgtaccta acttgattgt
    34801 tccaaaatca agatctggag cttggtcaat cgtcagtaat ccggatgttc cctgatttgg
    34861 ttcgtcaggt gtaaaaggag tatcaggatc atttggatca acaggaggaa ctggaacaac
    34921 gtcttcagga tttgcagcat ctggtttggt cacagaaatc gttccttctg tagtggctga
    34981 gctcttattc tcagcaaaaa cactttgtgt acataataat agacttccag ctagaacggt
    35041 acttaatgca attgtttttt tcatttcttt ttccttcttt cttttaaaat aaataatcca
    35101 gcaaataagc caataagaat gactccaaca agtatcagcc atcgccagtc aaatgtttct
    35161 ggctgatttt ttaaatcaat atccttttca ttcaattggt ccgctgcatc atcagttatt
    35221 tcaaaatcgg tatggagatt ccagcgtttt tctttccaag cacccttcac aaccgcttga
    35281 taatgtcccg gagctaattt ttttccttct aatgggacaa gtaaatcaaa atttgtattg
    35341 ggtgctacat ctattttttc tttttttatt gaaaggtaag gctcttttgt ttcttttttg
    35401 tagatttcaa ccgatgtagt caagccgaac atataagaag catttggatt ttgaagattc
    35461 gccttaatca catttcttcc atcgctttga tccgcataag ctttttttaa tttcaaatct
    35521 ggggaagact ctttttcgct atttcaaatc aagattgctt tcacttgaga aaactgattg
    35581 acgacaccaa ctgtactttt gttagaaacg ttctcttttt tttcttgaaa cgtgatccct
    35641 cctgcgacca caccagccag ctctttttct ggcgctttca gtgaaagtgt ctcagtgacc
    35701 gcactttgag gaggaacagt tatttctccc actgtttttg ctaagttttt catatcataa
    35761 cttaatgttt catcaaattt actttttgat tctccatatt cgatcacacc gtttgaattc
    35821 gtcgtagcgg agtggatcaa tgtctcaact gttactgatt tatctgttcg gttaaagacc
    35881 gtgtagggga tttcctgtgt tttcccaggc tccatcctta catttatata tgttttgttc
    35941 ttatcaatct gattagccgg taattcaaaa tctaccgcaa aattgaattc ggaggcatgg
    36001 acaggtgtct gccaaaggat gaatccaagg agaaggatca taagtaaaga gcagttgttt
    36061 cgatgacttt tattccatat tcgattcatc aatcatcctc cttgatcttt attctgtcgg
    36121 cgcatctgct aatgtccatg ttatctctgc tgagtaatta ccagctatct gattttcttt
    36181 ggttaagaca agtttaaacg cccgaccgtt atttaaatat tctgaataat ctgcttgcac
    36241 attcgttgaa ttttgtcctc tgacaatttc cagttcttct tcattcacta attgatactc
    36301 ctctcctttc ttgaaatata ggacatcctg catagaaaat cctgcatcat ttgtgaaagg
    36361 ctgagataat tttatcgtta agatccacgg atttttcgtt tctcgctgat cataaatcca
    36421 taatttttcc tgtatatcca tttggccaaa tgcttcttgt gtcaaaccag ttgctttgat
    36481 ctgaccaaaa tcgagtttgg aaggggcaag aaatgagaga ccaccttcta ctacgatctt
    36541 cgtagcaggt gcaaaaggcg gggcatcatg ataaacaact ggcgtatcac ttggattgtg
    36601 attcccaatt tcatcgttgg tacttggctt gttctgcaca cccttagcag tagcttctcc
    36661 tgcttggtcg tttaatacaa tttctaatac atctcctgca aaaagttgat tttctgggat
    36721 agaaagaaca aattcccctg tatcatcaat agttgtttct gacaaaacag cttgattttt
    36781 tttcaatgaa accaaggctc ctgcttcacc tgtacctgtg atcgtgcgag aagctgcatt
    36841 gatcaatgtg gtacttaaat tagcaggttc aggaggaatc acatcgactg tcgttttggc
    36901 agagtccagt tctgtcactt cttggccacc tcttactttt ttagcagcaa caacttgcac
    36961 ttgatcctcc gtttttaatc tctcatcatt gggtacttga acttcgaaca agccgccttg
    37021 ttcgccttct ccccatgggt tatatcctgc cccttcattt tcatctgttt gtccttgcgt
    37081 gagagcagta atttctacag atgatttcgt cgtatctgcg taagtaatac gtaaagtcac
    37141 ttctacttcg cctgtccaag catctcttgg ctttgcctcg acaccttcgg gaacaacgac
    37201 atgcccatag atttttttat ctgcattcgt tggcactctt aaggattcaa tcatcgggag
    37261 ctgattattg gcactgattc gattataatt tttcattccg gcacctgtct gagcaaattc
    37321 atttgttaaa cctgcactag ggcttgatac gaaattgcct aagtcggtcc cattgatcga
    37381 taagtctgtc atatcagaaa aataatccgg gtctttttca aagttatcga atgttgcgcc
    37441 tgtttttcgc cataaagcga tttctgaatg ggcgatcttt aaagaagaat ctccatttcc
    37501 ttggaatacc cagccgccta tgccattggt ctcgcttcca atccgtttgt tttggaaatc
    37561 aaaataggta ggagattcta agtccacatt caacacatcc ccactgatga tgccggtctt
    37621 atcttcccct gttttcccga ccatgactaa ataagttttt tcacctgccg taaaatttaa
    37681 atcaatatct ccagttgtat ctacagttgc tccggaatct gcgcgtatat cgatatttga
    37741 tttttctcca gtgactgtaa aatcagctga acctggactt tctagaaaac cagaagcgtc
    37801 ataaaattgg atcgcttgat ttccttgatt gagtctgttc acgtaagatt tgccatcacc
    37861 tgtatttctt accctcaatg agcctccatt ggttactgtc agcttttgcg taccattctc
    37921 aaaacgaact gcgctggaac gcccactctg cttatcgatg tcgataactg atccggctcc
    37981 ttctacgatc aaagaaaggc cttttcctac aaaacgcatc ccattgttcc cgggttgatc
    38041 gtcatctcca gccattttga ccgagatttg tccaccactt gatacatgga tttgactaga
    38101 atctccttgc aagcgaaatc cgcccgtagt tgctgtattg atggtgattt tcccaccatt
    38161 cgatacagtg actttcgatc ctgaacctct catttggatg attccgcgat tgttgtcatt
    38221 ggtaatattg atctttgtat ttaccgaaaa ttcgcttccc gaaccactga cttcaaacat
    38281 gacattacca ggtagcgtat cgactgcctc tccttcatcg ctaacgccat ctgcccgatc
    38341 ataatcaaga gagacctttg aaccgcttaa gatatggatt cctggcgctg tttttccact
    38401 accagtctca tctcctgaat ctagaaaacc gtagaatttc cctagatacg gtccataaag
    38461 ttgactacca ttgatcacat tcaattgata gcttctacaa aaataggctt tacccggagc
    38521 ggcattcagt ccttccttca ctggttggac atccaacaca ccggaaacac catctaatgt
    38581 gacagttgca cgaggagcgt aaatcagtcc taatttggac ccatcataca aatcaagtgt
    38641 cccggtaaat tttatttctc ccgtaaaatg atatctgctt ccccaaaaat cggtcatata
    38701 agcaatgata atggcatttc catcgatatt tggtgtcaat ccacctggat gtgcagctgt
    38761 tcctttaaaa ttctggaact cgatcgactg aggtatctca ttgatataaa tatggtgtgt
    38821 gccgaaattg tgagcgaatc caccaaaatc aataacgata tctcgtttga tctctaattc
    38881 cctgtctaaa gtcaaaggct gaatcagtcg gatcttagtt actgattcgt ctgcgagaag
    38941 ctgcgccagt tcttctgttg tggcagcttc gctgtaccca tccgctgaaa cagctcttgc
    39001 cagtgccgct tcactgctat cagacgtttg ttcagctact tctgaggtct cacttgtctt
    39061 attttcttct tgccttttat tagtagaagc ctcactttga gattctgtag tttcagaagt
    39121 aatcgattct tctgaaaaag tagtcttgtc aaaactttga ttctctgatt gcgttgattc
    39181 gtttgttttc tctacatcgg caaaaacgga tgtcaccatc aagagaggga agacaaacaa
    39241 aatcgacgca atcagctttt ttacacattg attcattctc caccatcctc tctgacgttt
    39301 tgacaatttt attttatcgt tttaatctat ttccaattgt actttcatat ttaaaatatg
    39361 tatttttatg gaaaaatttt tctattaggt gtaaatttct tttttgtaca aaaaaaaggc
    39421 tgtgacaaaa gtcttgtcac agtcccttat cccgaacaag cggtgttctc ccgccaactc
    39481 ctcggtcaca acctccattt taacaataaa cctgtaaatg tgagggcaat actttgattg
    39541 tcacaggtaa tttatcccct tcatctccat caacattcgt cgtcaattct tcctcatcag
    39601 ctaatgacac aactaattgc tcacaagtaa ggtaggctag atttttcgta gattcatcta
    39661 ctccttttaa taggtctggc aatgttttaa gcgtatcgat caaagaagaa tcttttaaat
    39721 aaaccaaatg gagcttacca tcatccactt ttgcatttgg aagtaatgtt tcaaaaccgc
    39781 cgactgagtt agttagtcct atcagcaagg tactgctctc aatttcttcc ttctttccat
    39841 caacttctac gtgaaacgag taggattgtg tggaagctaa ctgtttgatt cctgacatga
    39901 aataagctag ttttccaaat ttcgtcttct tctccgcatc tacatcattg attgattcag
    39961 ggatagagcc aattgcgacg acattcataa aatagtcgtc atttattttt ccaatatcca
    40021 atggcttgac tgattcaatc gaaaaatgat tgatcgcttc ctcaggttct agcggtatcc
    40081 ctaatgctcg agccaagtca ttcaccgtac ccaatgggaa aaatccgaaa tttggccgat
    40141 gttcttgttc agcgattcca ctgattcctt cgttgactgt tccatctccg cccatgacaa
    40201 acacactatg atacccttcc gtagccgctt cgcgagtaaa gttttttgca tcccctgctt
    40261 tttttgtatg caagacgacc acttcatcaa acaccgattc caattttgct atcgctaatt
    40321 gctcaaattc ctttgcttgt tcaccgcctg aactggggtt taccactaat aaaacttttt
    40381 tcatcacttt ctttcccttc tgattgattt ctttaatata acgatagcat aaagaaaaaa
    40441 attaggctgt gatgaagtac tgttcatgaa acattcattt attttttgac aagataaaac
    40501 atccatacta tgaaactgag gaaaattaaa acaatgatat aggaaataaa agaaaatgat
    40561 aggcattgta tacctaataa agtaaaaatc ttaggtggca gataaaaaca caaaaatagc
    40621 aagagaatgt aatagcgttt cttccacttt ttgttatcag accttatagc gatcagagga
    40681 ataccaaatg cataactgaa aaactcttga tgtactgtta atatgtcacg gccttgaata
    40741 ataaatgcga caatccccgc tataattagt gtgcctaaca ccaaaacgga aaattgatga
    40801 atatatttta tatacaaatg atccattgta cttcctgcct ttttatttgt gagaaaaaca
    40861 aatccgtata cgtgaataac ttctagaaat aaaagaaatt tgcaaaacca ctccatttat
    40921 ctacttctac gtgcattttt tgatattgtt ttctttattt aagattatac agaaaaatat
    40981 ctagggtcgc tggattctaa gtaaaaaaaa tttttaaaat aaaaagaact aaatcaagta
    41041 gtaattaaac tactgttgat ttagttctaa taaaaagatt tagatgaaca gttttattga
    41101 tttaaatagt tcatattttt tatttatacc gcttatttac tacttgctgt actagaatct
    41161 gaagtgaatg tactcaaaac attttcaaag tctttgtctt tgatttttac attagcgtct
    41221 ttcaatactt cgccaattac tttattctgg aaggtactgt cacttaattt tgtgtctgtt
    41281 gcaatttctt tcaattcttt tttgtattta tccatgtcgt tgcctttgtt ttggtttttc
    41341 accattttaa ccacataata ttccgtagta taagttgaag cattggtaga agtgatcaca
    41401 tcagaaactt gaccatcttt caacttaaag gctgcttctt tgacttctgc cggaaccgtt
    41461 gtagtagtgg aatcaaattt cacttttccg ccatcttctt ttgtcgtagt atcagttgac
    41521 ttgtctttgg ctaatttaga gaaatcgtct cccttatcag cagctttctt cacatctttt
    41581 gcatcatctt cacttgctac ttggataatt tgagcttcca cttctggatg gaaagattcc
    41641 caagccgttt ttaaatcttc atcggtcaat ttgatatgtt tctttagacc ttcttgaaag
    41701 gctagacttt gtttgatact gtctttgaat gtttgttctg tgtaacctgc agatttcaat
    41761 tgtgaatcaa atgaatcacc caattgtttc tttgtttgat catattgaga atcgatttct
    41821 ttggttgata cgtcatctcc atatttatct tcaaatactt gataaacgat caactgttgc
    41881 acagcggaat ctgctccttg catttttttc aagctatcca ttacttcttg ttcgctgatt
    41941 gtaccgcctt tataactaac ggctgttttt tcaccggcat tcgtacagcc tgacaataag
    42001 ccagtgccta gtacgatggc aagtgctaaa aatctttttt tcatttatca attctcctac
    42061 ttttcgtttt ttgcgaattg ctaaaaacaa aggtgttcaa gatgaaaatc atcaagaaca
    42121 cctttactaa tctgctcgat acgtcctgaa tagatataag gctacacaat ccagaaagtt
    42181 cgctcagcag aataaatgtc aagtagcaca tcacacctta atatactact tcttttgtta
    42241 ccgtttttca agtctatctt gcgttccgta tttaacgtaa ctaattctta gaacaaatgt
    42301 tatcgatttt gtttttcttc ttccgctgct gggatattca aatcagacac ttttatgttc
    42361 gattttccag acattttttt gttgttttta ttatgatata tttttataca actaaaaatg
    42421 aatatattaa aactaccaaa aataactttt aacgctgttt cattatatgt tttttctatt
    42481 ttatataaac ttttggatac ttaataccaa ccggttggat tgtaccaaca tgagaatgtg
    42541 tcagttctat ttcctgtcgc acttgcatag gcccatactc cttttgttgc atttgctgat
    42601 cgaatagagt ttccaacttt cgcttttgca ccgtgacgat tatagacacg actatagtgg
    42661 ttagaatgac ctgtttttct cgtacaagtt gtatcactat aataaaccca tcttccagag
    42721 ctatgattaa ttgctgttct agaaatttcc cggtctactg gtaaatttga cttaataata
    42781 ttacctttag catcaaaatg aatctctcct ggctttaaag atttgtaata gatttcaccg
    42841 tgaactgaat ttgcttccac ttcagatgac atagcgtttc ctccaacaaa acctatccct
    42901 agtgcacaaa taataactgc ttttttcata aaacgacatc ctttcctttt tgatttttaa
    42961 agcatgatct tcttgtttcc tattttggtc agatatggat catgcgtgac tacgataact
    43021 aacgtacctt gggaagcttg ttgcgataag atttcaacta ctttatcctt atttctttta
    43081 tccaatgacc cagtcggttc atcggctaaa atgattttgc tattcttcaa taagactctc
    43141 gcacaggcga ttctttgctt ctcaccacca gataatgtgt agataggcgt acttagcttc
    43201 ttgtttatat ttactttttt caatgcctca tactttgctg ttcgcttatc tgaaacattt
    43261 aaattcgaaa acttcaatcc aatatctaaa ttaaaatcga ttgtttcata ttcaatcaac
    43321 ccaaaatctt gaaaaagaaa agatatggta tttcttttta tcaagcgctt ctctctccca
    43381 gttaattgtt tcgtctcttg atcgaacaaa tagaattgtc ctgaactgaa ctgatcattc
    43441 aatcctaata tattcaataa cgtactttta cctgctccac tttctccaac aacagtcaca
    43501 agatccccag catccgcagt gaaacgatcc aaatgaagaa tcgttttgtt tcgaatgatt
    43561 tttttcgcac cttttatcac tatcaacggc tccaattgtt aacctccatt catcagcaat
    43621 aactgacttt tcttttctgt agacaaaata aaaacaactg aaagaataaa atcaatgaag
    43681 ctaaagaaaa aaatattaaa aagatattta ggctctcttt ctataagaaa aaggaagatt
    43741 acacttgtcg ttccccattt gatgaggttc atcatgaaga aatctttata cgtttcaaag
    43801 agcgaataac cacttgaacg aaagatagcg attttccgac aattgtagac aaaatatatg
    43861 cataacatga catagctcaa caagaagaaa gaaaagaaag tgagtaaaaa tatctgtatc
    43921 aagtaatgaa atgatctgct taacactttg acagctatct cttcagtctg agtatacgga
    43981 aatgataact gcaagttatc acgtaactga ctttcttcaa tcagctcaaa taacctttct
    44041 ttcgacaaag agcctatctt gatctttaac ggtggataga tttctccgct gagaatattt
    44101 ctttccaaat acatactgtt tttcaatgtc agtatttcaa caaccggata atgttcgatc
    44161 caggggatag aaggaataaa tgtataaatg ggttgattgt tttttataaa tagaaatttc
    44221 ggttcttttt cactgatttc ttgataatat tctgttattt gcgccaggtc attttttaaa
    44281 tctgtatgtc tatatcgttc agggatcaat ataacagggt gcgtttcttt ttcggttatt
    44341 tggatcaacc ttccagagac atcaatcaaa ggaaatttct taagataatt cagatttatt
    44401 tttatccctc tgccaaactg ttcattttct tcattcaagt aatctgatac attgactaat
    44461 atacttccgt ttttattcaa agtttcatat aattcctgat ctgctgctga tgcaaaacga
    44521 ttggaataaa taaaatccac gtggtttttc ccgatggtta ctggataaaa tacatgatag
    44581 tcctcaccag cgatttcttt tggtggttca tgggaaacag ctaactgtga agaagaaaaa
    44641 acgatagttg ctaattcgac attggcaacg gtcaaaagaa gagaaaacaa caatccaaat
    44701 gtaaaaagaa tgtattggat atagttgaac gcttctctta acgaagcgat tttttcaact
    44761 aataaaagag aaatcaaaga gataccatat attccgctga acaagaaaac gtacatctgg
    44821 aacaaataaa gaaattgctc cttataatac ataaaaataa aaccaccaat gattcctgca
    44881 attatgaatg taataatgat tttctttttc cccaataaac accacaatac ttgatgtaat
    44941 gaaaagtcat tattcaacaa taaactgatt tctttattca tcaataagaa aaaggatgat
    45001 aacaaaagga ataaaagaat caatccaata gttaaataca ctcgataatt atatacgtct
    45061 gtatttgaaa taaccgaata cgaatagcta ttttcaaagt cttcaaaaat attagtggca
    45121 taatcagtgt gaaatgcttg attgaatcga tcagtgaata gtgaaatgaa ccgttcatag
    45181 gcttcttttt ctctcgtcat caagaaaaat tcaccttcaa aatcttttat tttgatcgat
    45241 tcttcaatgg acgttacgcg aaaggaggta ttagaatagg gtaccttctc gtgagaagat
    45301 ttgacatccc cttgataata caatgtcatt tcaagctttg gctgtatctt gaagttccaa
    45361 gatgtatcta cttcattgga ttcatttact gtttttttca tataagaaag ctcagtttct
    45421 gatgagacat tttcaaatac catgattagc tgctcatatt cttcttgccc aataaaatgt
    45481 tgtggcaatc gaatcggagt ataagtatct gtttgatgga aaaattcatt tgtccgatag
    45541 agtacctgtt tatttatact aaccaaggca aaaccactca tgataccggt cactatgatt
    45601 gtgagtaaaa ctgtgaactt ttttttgatt atcaccactc tcccttactt aaatgattct
    45661 ttgataaaat cataccgttt ttcatcaaaa aaacgtatct atcaagtgta taggtcatgt
    45721 agaataaatc tatatactgt agaatcgaaa ggagaagaag atgaacaccg gagaaaaaat
    45781 acgaacttac cgcagaaatt gtaagcttac gcaaaaagaa ttaggcgaaa aaattggtgt
    45841 ttccgataaa acgatttcta gttgggaaaa ttctcgtaca atgccagacc ttgaaatgct
    45901 gtcactttta catcatactt taggtctgcc gattgatttt ccttctattg aatcagctac
    45961 cgaatcaata gaaccaacat tgccagatga acctcttgtt aacaagaaat cttattcatc
    46021 aaaacaaccc ttctcactca aaaagacact atctttcttt ctgctcgtct tttgtttggt
    46081 ctatcttttt cttatcaatt atcaatacaa aaatgaaacg atcgatcgat tcaatccttt
    46141 tttgaaacca caaatcggct ataccacact ccctgtagga tacgtggaca attccatcga
    46201 acgttgggta tgtaatgata tctttggcca tggtacatgg ctgtcttttc acgctggaga
    46261 ggtagaaaaa gagaaaaacc atgctgtaat caaacacaaa ggaaaacacg tcatgaacgt
    46321 tcgcctgctc acttgggaag ccattccctc agattaccaa gaaatcctca gccaagacta
    46381 tgacgacgcc ttaaaaagaa gccctcagga gccagatagg cattaagtag gagatgaacg
    46441 tagagataga aaaaatcaat gcacatgatc ttatccaaaa aacaggggta caaattaatt
    46501 gaaaaagcct tgagatgaaa gttcctaaat aaaaaagttc agaatgatag gtatttctgg
    46561 cttatagtat aatatttacg ccacagcctc ccaatgattc tttttggaat cataatcaag
    46621 gatggacatt cacaaaaata ttttgctcat tcgatccgaa tagattaacg accaatctag
    46681 taagtaatat agattggtac gtagtcaagc tgcacataat ttagatcaat tttagttctt
    46741 ttttatattt ctatgatctg ctcatttact tttatatgat ggcttacagt aacataaata
    46801 aatatgataa tcgctggtag tttatccaag agtactcaat aaaatacaaa atggattgtt
    46861 caagcgccta tcataatgat aaaatgaaac aaataaacga tacaaggagg aagagacttt
    46921 gagtaatggc agaacaattt tttcattgta aaaattgttc agttgttaat ctatgggatt
    46981 tcggtagcat gggcattttt ttatagagga atcacaccaa cctggccgtt ttatctcttg
    47041 ttagtgggaa tattagtagg aaattttgcc cctcttcact atcgtacgaa cttttttcca
    47101 actgattctg gcctttttct taaaacaaag acgcgaaaat tagaaaacat tattgaaatg
    47161 acaattacct gtataatcat tttcttagtt atattcctaa acgtcttcta ataagaatct
    47221 cttaaaatta gcttgttaca gttagttacc tagtttatat aacacagtta tcttatcttc
    47281 cttatcaaca tcttttgcca actctttgtc ttaaattcga aacatataag cagagctacg
    47341 aacaccaaga ctgtttcggc tctgtttttt gcatcaaatt cactcttata tagctgataa
    47401 tctcggctct ttaaaagaaa ttattttcgc ttcattttat tataaaaagg ctaggaacct
    47461 tgatgttatc aggttcctag cctttattta agtatt
//
LOCUS       DAZBSA010000008        45005 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000008 DAZBSA010000000
VERSION     DAZBSA010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.008"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..420
                     /locus_tag="ACHGMT_000426"
     CDS             <1..420
                     /locus_tag="ACHGMT_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFN6834198.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(504..1226)
                     /locus_tag="ACHGMT_000427"
     CDS             complement(504..1226)
                     /locus_tag="ACHGMT_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFN6834199.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(1216..1953)
                     /locus_tag="ACHGMT_000428"
     CDS             complement(1216..1953)
                     /locus_tag="ACHGMT_000428"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6834200.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            complement(1965..2957)
                     /locus_tag="ACHGMT_000429"
     CDS             complement(1965..2957)
                     /locus_tag="ACHGMT_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN6834201.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            complement(2972..3715)
                     /locus_tag="ACHGMT_000430"
     CDS             complement(2972..3715)
                     /locus_tag="ACHGMT_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6834202.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     regulatory      complement(3789..3893)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3956..5278)
                     /locus_tag="ACHGMT_000431"
     CDS             complement(3956..5278)
                     /locus_tag="ACHGMT_000431"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFN6834203.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     gene            complement(5265..6230)
                     /locus_tag="ACHGMT_000432"
     CDS             complement(5265..6230)
                     /locus_tag="ACHGMT_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6834204.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            complement(6227..7765)
                     /locus_tag="ACHGMT_000433"
     CDS             complement(6227..7765)
                     /locus_tag="ACHGMT_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFN6834205.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            complement(8181..8468)
                     /locus_tag="ACHGMT_000434"
     CDS             complement(8181..8468)
                     /locus_tag="ACHGMT_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834206.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            8602..9270
                     /locus_tag="ACHGMT_000435"
     CDS             8602..9270
                     /locus_tag="ACHGMT_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN6834207.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            complement(9561..10301)
                     /locus_tag="ACHGMT_000436"
     CDS             complement(9561..10301)
                     /locus_tag="ACHGMT_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834208.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(10431..11378)
                     /locus_tag="ACHGMT_000437"
     CDS             complement(10431..11378)
                     /locus_tag="ACHGMT_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN6834209.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(11507..12271)
                     /locus_tag="ACHGMT_000438"
     CDS             complement(11507..12271)
                     /locus_tag="ACHGMT_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFN6834210.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIQLMEEASTFLAKNLIKKYK"
     gene            complement(12275..12664)
                     /locus_tag="ACHGMT_000439"
     CDS             complement(12275..12664)
                     /locus_tag="ACHGMT_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834211.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            12799..13341
                     /locus_tag="ACHGMT_000440"
     CDS             12799..13341
                     /locus_tag="ACHGMT_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFN6834212.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13489..14121
                     /locus_tag="ACHGMT_000441"
     CDS             13489..14121
                     /locus_tag="ACHGMT_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834213.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(14255..14380)
                     /locus_tag="ACHGMT_000442"
     CDS             complement(14255..14380)
                     /locus_tag="ACHGMT_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFN6834214.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(14514..15407)
                     /locus_tag="ACHGMT_000443"
     CDS             complement(14514..15407)
                     /locus_tag="ACHGMT_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFN6834215.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(15589..16515)
                     /locus_tag="ACHGMT_000444"
     CDS             complement(15589..16515)
                     /locus_tag="ACHGMT_000444"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFN6834216.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(16600..17361)
                     /gene="pflA"
                     /locus_tag="ACHGMT_000445"
     CDS             complement(16600..17361)
                     /gene="pflA"
                     /locus_tag="ACHGMT_000445"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFN6834217.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(17605..19836)
                     /gene="pflB"
                     /locus_tag="ACHGMT_000446"
     CDS             complement(17605..19836)
                     /gene="pflB"
                     /locus_tag="ACHGMT_000446"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFN6834218.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(20113..22563)
                     /gene="parC"
                     /locus_tag="ACHGMT_000447"
     CDS             complement(20113..22563)
                     /gene="parC"
                     /locus_tag="ACHGMT_000447"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFN6834219.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(22575..24620)
                     /gene="parE"
                     /locus_tag="ACHGMT_000448"
     CDS             complement(22575..24620)
                     /gene="parE"
                     /locus_tag="ACHGMT_000448"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFN6834220.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(24786..25220)
                     /locus_tag="ACHGMT_000449"
     CDS             complement(24786..25220)
                     /locus_tag="ACHGMT_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFN6834221.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            25347..25985
                     /gene="plsY"
                     /locus_tag="ACHGMT_000450"
     CDS             25347..25985
                     /gene="plsY"
                     /locus_tag="ACHGMT_000450"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFN6834222.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(26117..26992)
                     /locus_tag="ACHGMT_000451"
     CDS             complement(26117..26992)
                     /locus_tag="ACHGMT_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFN6834223.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(27074..27865)
                     /gene="codY"
                     /locus_tag="ACHGMT_000452"
     CDS             complement(27074..27865)
                     /gene="codY"
                     /locus_tag="ACHGMT_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFN6834224.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(27883..29283)
                     /gene="hslU"
                     /locus_tag="ACHGMT_000453"
     CDS             complement(27883..29283)
                     /gene="hslU"
                     /locus_tag="ACHGMT_000453"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFN6834225.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(29297..29845)
                     /gene="hslV"
                     /locus_tag="ACHGMT_000454"
     CDS             complement(29297..29845)
                     /gene="hslV"
                     /locus_tag="ACHGMT_000454"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFN6834226.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(29882..30787)
                     /gene="xerC"
                     /locus_tag="ACHGMT_000455"
     CDS             complement(29882..30787)
                     /gene="xerC"
                     /locus_tag="ACHGMT_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFN6834227.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(30985..32361)
                     /gene="trmFO"
                     /locus_tag="ACHGMT_000456"
     CDS             complement(30985..32361)
                     /gene="trmFO"
                     /locus_tag="ACHGMT_000456"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFN6834228.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(32330..34408)
                     /gene="topA"
                     /locus_tag="ACHGMT_000457"
     CDS             complement(32330..34408)
                     /gene="topA"
                     /locus_tag="ACHGMT_000457"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFN6834229.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(34530..35387)
                     /gene="dprA"
                     /locus_tag="ACHGMT_000458"
     CDS             complement(34530..35387)
                     /gene="dprA"
                     /locus_tag="ACHGMT_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFN6834230.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(35443..36210)
                     /locus_tag="ACHGMT_000459"
     CDS             complement(35443..36210)
                     /locus_tag="ACHGMT_000459"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFN6834231.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(36203..37063)
                     /gene="ylqF"
                     /locus_tag="ACHGMT_000460"
     CDS             complement(36203..37063)
                     /gene="ylqF"
                     /locus_tag="ACHGMT_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFN6834232.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            37323..37721
                     /locus_tag="ACHGMT_000461"
     CDS             37323..37721
                     /locus_tag="ACHGMT_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN6834233.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            37958..38500
                     /locus_tag="ACHGMT_000462"
     CDS             37958..38500
                     /locus_tag="ACHGMT_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFN6834234.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(38583..39134)
                     /gene="lepB"
                     /locus_tag="ACHGMT_000463"
     CDS             complement(38583..39134)
                     /gene="lepB"
                     /locus_tag="ACHGMT_000463"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFN6834235.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(39286..39513)
                     /locus_tag="ACHGMT_000464"
     CDS             complement(39286..39513)
                     /locus_tag="ACHGMT_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFN6834236.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(39510..40028)
                     /gene="msrA"
                     /locus_tag="ACHGMT_000465"
     CDS             complement(39510..40028)
                     /gene="msrA"
                     /locus_tag="ACHGMT_000465"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFN6834237.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(40048..40761)
                     /locus_tag="ACHGMT_000466"
     CDS             complement(40048..40761)
                     /locus_tag="ACHGMT_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFN6834238.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(40764..41660)
                     /locus_tag="ACHGMT_000467"
     CDS             complement(40764..41660)
                     /locus_tag="ACHGMT_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFN6834239.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(41818..42660)
                     /locus_tag="ACHGMT_000468"
     CDS             complement(41818..42660)
                     /locus_tag="ACHGMT_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFN6834240.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            42792..43445
                     /locus_tag="ACHGMT_000469"
     CDS             42792..43445
                     /locus_tag="ACHGMT_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFN6834241.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(43508..44035)
                     /locus_tag="ACHGMT_000470"
     CDS             complement(43508..44035)
                     /locus_tag="ACHGMT_000470"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFN6834242.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(44055..45002)
                     /locus_tag="ACHGMT_000471"
     CDS             complement(44055..45002)
                     /locus_tag="ACHGMT_000471"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFN6834243.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
BASE COUNT        12261 a         9400 c         7757 g        15587 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacaac ctgttgtaca agaaacggtt
      121 caaacggaaa aacaagcagc gcctgttgct gaaacacaac ctgctcctgc tgtaacagaa
      181 actgctgcta ctccagcaag cacaagctct gcaaaagaat ggatcgcaca aaaagaatca
      241 agcggttctt acacagcaac gaacggacgt tacatcggac gttaccaatt agattcttct
      301 tacttaaatg gtgactactc tgctgcaaac caagaaagag tagcagaaca atatgttact
      361 tcacgttatg gttcttggga agcggcgaaa gcattctggg aagcaaacgg ttggtactaa
      421 gattcattta ttaatcaaac agtttacttt tatacaaaga aagccaaaaa gaggtgcgac
      481 actagttgtg tcacatctct tttttattta cctaatctaa tgaaacgttc caagttttga
      541 taagtatttt ttttaacttc ttcaactgat agctctttga tctcagcgat tgtctttaac
      601 tgttcttcaa gcagtgagac ataatctttt tctgaaaaaa tcccccctaa agcccactct
      661 gctgcatgta gtccatccgt ttcaataagt aagtggtcga ttgggactgc tttagctagt
      721 tgccagactg ctggattctt tttcaaatca agaccaatgc tgaaataaca gcctaaatca
      781 atatagtctt gttgatagtc caaagaagaa taccaatgga tcaaatagtg attagggtaa
      841 gcttggatat aatttaagat ctctttttcg catccttttg tgtgcagtac aacaggtttc
      901 tcatttatgg ctgcaaaagc aagctgtcgc tcaaatactt ttttttgtgt agtcatcggt
      961 acattcgtcc aaatattatc taaccctatt tcaccgatga tccttgcttt tttcaaaaaa
     1021 ggctcaactt gttcaaacgt atagctatct gctttccaag gatggatacc gtaacttaag
     1081 gcttgagtct tacttgctgc aagctgtctg ttttccttcc attcttctgg cgaatcacaa
     1141 ttgatgatcg tcaaaatcgt gtgttcctgc tgaaggagtg ctaaactctg actcccatct
     1201 aaatgacaat gagcatcact taacatctaa ttcacctgcc aataactgat aaatcgtttg
     1261 cttcaactct gattgctgca gtagccattc acgtgaccgt cgtttttcag gcacttgaat
     1321 ctcagcaatc acatgtgccg gacgggcgga aagcacaaga atcctgtctg ataaataaat
     1381 tgcttcttcg atatcatgcg tcacaagtaa agtagtccgc tttgcttttt taggcagctt
     1441 caacatccag tcttgcatct gccctctggt catagcatct aacgctccaa atggttcatc
     1501 aagaagcaag taagaagaag gattcatgat tgttcgtaac aaggccgttc gctgcttcat
     1561 cccgccagat aattgttccg gcaaaaaatg ttcatagccg tttaacccaa agatatccag
     1621 ataatccctt acttgttctt cattgattgt tagatgattg attttttgat ataacgtaac
     1681 gttttgatag acgctcagcc aaggaagtaa catatcttct tgcggcatat aactaaatgc
     1741 tgtatggaga ccagttactt tttgatcgtc tactttgatc gttccagaag tcataggctg
     1801 gactcctgtt aaagttttca gcaatgttga cttaccggct ccgcttggcg cgacaatcga
     1861 tgtaaaggta tctttaggga tagtgaaatg gatgtcttct aatattttca ggttttcaat
     1921 gactacattc aagtggctga tttggattga catatctatc actcctattt tataaactga
     1981 ttagtgaata gttcttgcga aggtacatct tgcttgatca atccgttttc ctgcataaaa
     2041 gctgtataat tgcgccaaac tttttcttcc atcaatcccc aattctcagg atcatccgta
     2101 taattttttg acaagaatgc ttgtgattct tctaaaaacg tccgatcatt ttcttttgca
     2161 tatttttgca acagtttagc actgtcattt ggatccttaa tagcttcctg atagcctttt
     2221 ttcgtggcat ccataaaaga ctgtaccaat tctggatcac ttttgatcag ttgatcattc
     2281 gtaataatta ccggagtata gtaatctaac cgttcatcta attctttcaa tgggatatag
     2341 tttagttcaa tacccgcttc ttttgctttg atcatatccc aaccttcaaa gtaccattgg
     2401 atatctgagg attttcctaa gctttctggc ccttctccat tgcttcctac cattgtcagt
     2461 tttgaaaagt ccccgccgtc tttttccatc acagctttta agacagcttc ttcacttgga
     2521 ctttgccaac ctgcatatgt ttttccttca aaatcttttg gtgaatggat gttactgtct
     2581 gatagggtgg cgaatccaga agtattatgt ttgatcactg tagcaattgc tttgactggt
     2641 atattctgtc catctgcgtg agcataagtg acatcttctt gataacttac accaaattgt
     2701 gctttgtccg cacctactag accaatactt gtcgaattat ctcctggttc gatgatttgg
     2761 acatttaaac cagcctcttt ataatagcct ttttcttttg ctaggtagat gccagtatga
     2821 ttagtattcg gtacataatc caaaacaagt gtcacttttt tcaaatcctt tatttcttgt
     2881 ttgctacttg tttcttttgc agtgccacaa gcggataaaa gcaacagtag cggtaataaa
     2941 agccataatt ttttcatttg atccccttct cttaaaaaat ataaagcata cgataatacc
     3001 cttttttcaa taatgaacaa gcgccattca gcaataaact caatagaatg acacaaatga
     3061 tcgcagcaaa aactttatcg atctggtagc tgttttttac gcgtatcatg taatacccta
     3121 agcctgcttg agcgcttagc cattctccaa cgactgctcc gctgacacaa taagtagcag
     3181 ccacttttaa accggaaaag aagccggata aacccgctgg tattttatat attcgataaa
     3241 tcttccctgt atctgccccc atcgttttca aaaagatgat ttgctctttt gatactttcc
     3301 tcaatgtatc cgaaaaagaa actacgatcg gaaaatagct tatcaagaca acaaggatca
     3361 cttttggaag cataccgaaa ccaaaccata gggtaagtaa tggccctaac accatgattg
     3421 gcaacatttg tgaaatcact agtaacggat aaaaagacat ttctaaaaac ttccaataat
     3481 ccattgccaa ggcagtaata aaagaaagaa cgctagctat taccaaacca agcactgctt
     3541 catttaatgt cgtgacagaa tgggaaagta atacttgtgc atctgccgct aatgcagaaa
     3601 atacagcagc aggtccgggt aaaataaagg caggtatttt ccaaactatg acaacaatct
     3661 gccaaataat taaaatgaat gccatactga ctgctggata aagatttttc ttcattttgt
     3721 tacctccttt cttctaatat gaataacaaa aaacgcttga aatgggcacg acatagtaac
     3781 ccgcttcaag cgtctgctat gtttctccct tcaccagtat taactgacag gttcaatggg
     3841 tctaagttca caacttactc tcatgtcgtc tacgacatcc ccagaaacag gtattcattt
     3901 tttaaagtgt agcacaagaa ggaaaaagca acaagcgctt tagttatcat gctgattatt
     3961 tcaataaata ggagagatca cttgccaatg tcggataccc catgatatag ctatccaact
     4021 cttttttagt caatttttga ttgatgacga gtgtcaaatc atcgatcaat tcgtctgctt
     4081 gctcgctaat cactgcagca ccaatcaaat aattttgctg gtcaaaggtc aattcagcct
     4141 tagcaactgg ttcattgaca cgatgatagg tgaaccaact ggtaagatcc atctctacca
     4201 cttgatctga cgaagaggcg tgactttttg taactcctac ttcagcaagt ttaggactgg
     4261 cgtagacgat cgtaggtata atcggatatt tgataggttc gcttgttgca tccgttatcc
     4321 gcttagctac ataatttcct tcaaaagtgg cgactggtgt caattttggg gttttccttg
     4381 aaacaatatc tccacacgca aaaatttttg gattgctcgt ttgcagataa tcattgactg
     4441 caattccgtg tttatcaaag acgatattcg cttgctcaag tgctaaagat tctgtattcg
     4501 gctgtctccc agtcgcacaa aaaatcatat ctgctactaa ttccgtctct tttccgacta
     4561 aacgatagcg agtaccttct gaaatgatct tttgggtttc aacaccaaaa gcaaactgaa
     4621 tgccagatgc ttccatttga tggacggctt cctctaccaa agacgcctca aattctttta
     4681 atggccgttg attatgatgg acaatggtta ctttgctccc tgcagcattg gcaatcgtag
     4741 ctagctcaaa agcaatataa ccaccaccga taaaaatgat ttctttaggc agtacaggca
     4801 atgataaaaa gtctgcacta gttttcaagt attcctttcc ctccactggt aagatcgtag
     4861 gtctttgccc ggttgccaaa actaagtaat ccgcatggaa gacttcttcg ttgacttcaa
     4921 tagatgaatc atccaaaaaa cgagcggttc ctgaaagatg atcgatttca gcttctgcta
     4981 attgtttttt tcggctttct gggactggat ccgtaaatgt tcgtttgaat ttttgaagtt
     5041 cttcccagtt ggcagttggg atctcgttaa aaccttttcc actcaattgt tttacccgat
     5101 tacgtgcctc tactgcactt aacaagactt ttttaggatc acatccacgg ttaggacaag
     5161 tcccgcccca tagatcttct tcgaccacta gtactgtttt acctgcttct ttcaatccat
     5221 aagcagcact taagccgctc acacctgaac cgatgatgat tgcatcatat gttttcattt
     5281 ccttcctcct ctcgacttaa aaaagcaaag atttctttac ttgtcagcga cccaatactt
     5341 ctttgctttt ctactatcac aatgcttgga tacaccgaaa gaagcgaata tacttctttc
     5401 ataggcgtat caaagcaaac ttgttgacga gcattttctg gtatttcatt taagaaatat
     5461 ccattgacga ctaaatcttc tactcgccac tctttcgtct gtttcggttc atcaaagaaa
     5521 tgccgaacgt agtcgttttt aggattcatc aataactgct ctggtgtgtc acattggata
     5581 atttctccat ccttcataaa tgcaatacga tcacctagct tgattgcttc ttccatattg
     5641 tgcgtaacga aaacgatggt cgtgcccaat tcttcgtgta aggacaaaac caattcttgt
     5701 aatgagtttc ttgaaatcgg atctaatgcg ctaaatggct cgtccatcaa aatcacatct
     5761 gggcttgcag caatagctcg gatgatcccg atccgttgct gttctcctcc agacaactct
     5821 ctaggcatac gatctcgata aatatcagga gataagccaa ctttttgaag aagttcatct
     5881 acacggtctg ctcttttctg cttttcccaa cctaacattt cagggatgac ttcaatattt
     5941 tgcttgactg tcatggtcgg gaacaaagcg atttgctgca gcacatatcc catattccac
     6001 cgcattttct gcagattata ttcatttaac gattttcctt tgaagtagat gtctcctgct
     6061 gatggtttag ataatccatt gatcattttc aaagtagttg tttttccact accggaagga
     6121 ccaaccaaaa caaagatttc tccttttttg attgccagat ttaggtcatc gatgacatac
     6181 tttccatcat actttttttg cacatgtttg aactcgatta atttagtcac tgatcttcct
     6241 cctttactaa tccttcattt ttcaggaaga ttcgtgctac ttctgtcggc tttttctttt
     6301 cgacattgac ttggtaattc atatcaatca tttgatcctc tgttattttt ccgcttaatt
     6361 tattcagtgc tttaacgatt tcaggatgtt ctttagcaaa atcttttttc atcagcggtg
     6421 cgccttgata agctgggaat aaccctcgat catcttctag agtcactaga tcgtactcct
     6481 tgagctggct atctgtggaa taggcatcca caatattgac atctccatta ttgattgctt
     6541 ggtaacgcaa acttggctcc atgctttgta cacttgaaaa attcaagcca tatgcttctt
     6601 ggattccttt atatccatct gaacgatcaa taaattccag tgtgaatccc gctttgatct
     6661 gatcagagat agctgccaaa tcggaaatct ttttaagttg atgttcgttc gcaaaagatc
     6721 tcttgactgc caaggcatac gtattttggt aagccattgg ttttaataat gtcatatcga
     6781 attgcttatt caataattct tctgcttcgt catatgtttt ttcttttgtc aaatcagcac
     6841 ttttcattgc ttcaggtacc ttcaccaagc tttctaggac agtacctgtg aactcaggat
     6901 agatatcgat ttgaccttga tccagtgcat tgaacaaaaa actcgtcttg ccaaaattcg
     6961 gttttaattc aacttctgtt tttgtgttgt cttcaatcaa ttctttatac atttggatca
     7021 ggatttcagg ttccgctcct aattttccag caatcgtgat ggtttcattt ttaaacaagg
     7081 cagactgata taaagaaacg ccgccaaata aacacaccac tgctgctaaa gtaatgatgg
     7141 aatatcgagg tttcttatgc tgcaaccagc gaatcagtcc gctgaatata attgcaagca
     7201 aggcagaact gatcgcacca atcaatgtca aaacaggtgt atttcggtca atacctaaca
     7261 aaataaatgt ccctaatcca cctgctccga tcaatgctgc caaagtagcc gtaccgatga
     7321 ttaaaactaa ggcagttcgg atacctgaga taatgacagg taatgcgatg ggaagctcta
     7381 ctttcagcaa gcgtctcatt ctggacatac caaatgctac cgctgcttct tcaatagacg
     7441 ggtcaatttc agctagacca atatacgtgt tttggaaaat cggaagcaaa gcgtaaataa
     7501 caagtgcaat caatgctgga acagttccga tccctacaaa tgggataagt aaacctaata
     7561 aggcaagtga aggaatcgtt tggagcacac ccgtgatctg caataagact tctgccattt
     7621 ttttatgatt ggctgcccaa atcgctaaag gaatagcaat cagtgctgca atcagcaaag
     7681 aaatgagaga aatggacaga tgttgaaatg cagcttccag caactcgcct tttctttcac
     7741 ttaatgtttg tataaaatta ttcatgcgac cttcctttct gttattcttt aagtataaac
     7801 atttctttcc tttttcccaa ctaacttgtc ttgccttaac cttacagcac gaacaaaaaa
     7861 aagaaaaaaa tacctctaaa tatgctgaca aaaaaagaat ccaagcaaca aagtcggatc
     7921 aactttttgg ttctcgtttt ttcactacga ataccataaa aatctatacg aaattgtcac
     7981 ttggaatcag ctatatcttt tttacacaca caacttttat tttaataaaa agaaacagcc
     8041 aataaagtag aaatctcgtg ggggagaaca cctttattgg cctgttcaac gaatattata
     8101 ctgtatcatg acactttttt gccaaaaatg taaaaaatgg ttgttttgac atcataaacg
     8161 tcttgttttc ttatttttac ttatatcacc actttatttt ctatctcttt cactccataa
     8221 agaggaatga gttcttcttt tgactgataa taaacagctt tctcacggtt cttcatcaaa
     8281 aaaagaaaga tgtctttcat agcaagtggt gctttgccgt gttcaaataa aaagataaat
     8341 tgatttgctt tatttatctg gaccattgtc acaggcacca gtttttgttc ttgagccaaa
     8401 aaaactcctt gatactctgt ttttttgggt gattttgtaa gaattgtcaa taagtctgct
     8461 gctcgcattt ttccactcct tatttgataa ataaaaagaa acagccaaaa ataatttttt
     8521 ttcattttcc tcttgaagta agagtataag tcagtataat aaagaagttg ctgaaaattt
     8581 tcaaaagaaa agaggctaga aatgaaaaaa ttaaatttat ttacagaatt gtgcggaatc
     8641 attttaggcg caggaatcta cggattagct gtgacaggaa tcaatattcc atctaagctg
     8701 gcagatggtg gggttactgg tattgctttg cttttgcata atctttttgg attcgctcct
     8761 tctgtcactt cactgctttt taatttacct ttattgctta tctcattgtt tctttttgga
     8821 aaacacgctt ttctccggac gatcgtcgga acatttgcgc tagttttctt tttacattta
     8881 tgggagagcc ttcatataca ttttgctgta gatagtctac tgatcaatag tttgatgaca
     8941 ggtattcttt ctggcattgg ttgcggtctc gtctttcgat tcggcggaag cacaggcgga
     9001 acagatatca tttatcaagc cattgaaaaa tattttcata ttaagattgg caaaagtctt
     9061 ttcttgatta cttttggtat tttagttgtc tccttgcttt atttggatct cacacatttt
     9121 ctgtatacat tacttagctg ttctattcta tcttatacat tgaataaagt gaagtatctg
     9181 gaaattggat ctgttttgcc ccaattggaa gcaaaacaga ctacagaaac atttgaacca
     9241 cttgatcctc ttgaacagtt agagcactga ttcttatttt tcactacttt atttaattta
     9301 cagaaatatc attatgtaaa ctaaaatgat attattcagt aaaatcttct ttataaaaaa
     9361 ataaactatt tttgcaaacg atctgcgaaa atagtttgtt tttttatttt gccattattt
     9421 tcttacttat tgggagaaaa aattcgctta aactcaaaaa aatttgagga tacgatataa
     9481 actttcggat agagtataaa ataaggaaac gccgcagcgt ttcctttcca actgtagccg
     9541 aatataaatt tgtatatcac ttatttaaaa tagctatttt ctcccaaaga gtttccttga
     9601 tcatcgatca atccttgtaa tgagcgttct aagataccat ggcttttttt cgattttttt
     9661 tcttctgact gctttgtttc ctctaacgtt tttgttcgat tgaatgttcg tacagacggc
     9721 tcaccatttc ttttttcttg atacggcgtc ggttctgtat cgaacaacag ataagagtca
     9781 tccgtttttt tcatcgattc gattaaatga tcctcagaaa ttttgctttc tctagggtct
     9841 gggataatcg atgcgggtac atatttagga acaaaataag aacgctcccg caccttcgtg
     9901 tcaacagatg cgtttcgtgt agtatgcttg gactctgtcc ttgtttcact ttgtctgaca
     9961 gaacgagagg aatcaccaaa aagttttttt cgtcctgtcg atgtgatttc tgattggtaa
    10021 tggccggtat aatccggtaa ttttccttta tgcttttcca aattttcctt acgcggagct
    10081 gacttcaatc catttgttgc acgacgcttg cgacggattt tctcggtgtt cgtgttcgtg
    10141 aaatgaaatt ctttcatctg ttcaaacggg gactgattct gttcagtcaa aagatcttct
    10201 tgtccgtcaa ataatactcg cttcttgtcg ttggttaatt ttacgcctaa ctcatcatcg
    10261 tacgcaggaa aacggaaatg ttttcgtctg atttctttca ttcaaaccat ccttctcaaa
    10321 aatggaactt ttctcaataa tagcacagat tcattgtttt cagtagcaat gcgctgatta
    10381 ttttaagaaa atagcgatga atttcataaa tatatatata gtaataagac ttaattcgtt
    10441 gtctctctga taactagttc aggaaaaaaa cgacgataac cttgactcgc ccctttttct
    10501 tctatttttt ttaacaacat ttctccacat acttcaccca tacgtataat atcttgcttg
    10561 atcgttgtca atttaggtga actgatttgg tctaaaaata ccccatcaaa tccaatcaca
    10621 ccaaattctt cgggcaagtg tcctccagca tttataatcc ctcttactgc tcctagtgct
    10681 agccgatctg aactacatac aaaacaagtt ttatctgtaa aatctgccca gttttctgtc
    10741 atatatcttt ctacttctgt cgaatgattt ggaaaccgca agattttact aggaagatca
    10801 acttccttca tcacagataa ataaccagtt tcccttgaaa tttcaaaagg ctcgtccact
    10861 tcgataccga tataaataat cgtttgataa tctctttcta aggcatattg cgttgctttt
    10921 gctgtaccat attcattatc gctgtctaca taatcatatc cttcagaatt ttccccaaac
    10981 aagacaactg gtttttccaa acggtcgatc caatcgaagt ctttttttct gacacctgtg
    11041 atgatgtatc catcacaaga accgacgtcg aaggcatcat ttgtaactaa ttggagagaa
    11101 taatgcgctt taccgatccg tttagcgatt cccatcagca aattcatgta atagggctca
    11161 gttgtgtcga tttcttccag tataaataat ttgatgatct gcgaccgatt cgtgacaagt
    11221 gcttttgcag caatatttgg acggtaatcc agttcagcca ttgctgagaa aactaactct
    11281 ttcaattcgt cagtcacctg ttccggatga ttgatgactc gagagaccgt catttttgaa
    11341 acattggcac gttttgcaac atctgctaaa gtgggcatgc tctcttcctt tctaacatgt
    11401 tgtcattctt actatcaatt ataccaaatt ttcctaacag agtttattat agcccaaaat
    11461 aaaaaacgga aaatcctcta tcgtcatgag tgattttccg tttcaattat ttatactttt
    11521 ttatcaaatt ttttgctaaa aaagtggaag cttcttccat tagttgtatt tgtaccatat
    11581 gtcgctgtgt ttttgaggaa cgaaaaacaa tttcttttcc gtaagactta tgccgatttt
    11641 tttctacaaa tcgttccgtt tgtatataag ggattttttc gtcagcttgt ccatgccaaa
    11701 agaacagagg acgtccagct aacttttccg gctgcaatga caaatcatag ctcttaaccc
    11761 atgaaatcaa atcaaaatag tcttttggta cgacataatt atattccgat gcgcgcctta
    11821 cgatttcttt tccatattcc aaaggtgcag gggaacccat caaacacacg cccgctacta
    11881 tatgaggatg tttagccaac aaggcgcatg ttgtcattcc ccccatcgaa accccgccaa
    11941 ctgcaagctg atcatgtaga agacctcttt tttctaaatg cttgataagt gtgtcaaatt
    12001 caaacagatt tccgtagata ctatcccaaa atgtatagga tggaatagaa gaaaccggct
    12061 gtttgcgctc cccatgattc atcgcatctg gcaggatcac acgaatgccc tgctgcgcaa
    12121 tttttcttcc ttgcgtcaaa actaactcct tacttgtctg ccatccgtga taatagatga
    12181 caacgggaat acgttcctct cttaattctt tcagaacaac ttctaacact ggaatatgat
    12241 ggacagaacg tttcaatata attggattca attgttactc acctattttc attaaatttc
    12301 ctgatcgcca tagcaaccat gctccaacag ttgtctggaa caggttcagt gtaccaaata
    12361 atataaatgc aggactgaat ggctcgggaa ataagcacag agaacaaagt cccaccactc
    12421 ctaatgctag cgaaaggagt ggataaatag aaaaattcaa aaaactggca acgctaccga
    12481 aaatattacc aataaacagt atcaaaaaga aaaaaacgct taaaattaaa ccatctgtca
    12541 gcgacatctg aaaaacaatg ttctctacct caattggctg ttgaatcgaa tagacaccta
    12601 agtatatact gagaaggaga cttagcacgt ggctggcaca aaacacacga ttgaccgttt
    12661 tcattttaat cacctaacct atctttataa ttctattata atcattttat catttttttg
    12721 tatgtataaa gtttcaattt ttcttcactt acgatttttt ttgatattgt tgttatataa
    12781 atgaaaggag ttttacaaat gattgatcca aaaatgttgg ggatgatctt cctcattaat
    12841 tttgcttata tcactctgaa cacgttacgt ttcatgttga caatgaaagg ctatcgactg
    12901 attgctccac ttgtcagtat ggtcgagatc acgatttata tcgtcggctt atcccttgtt
    12961 ttagatcgtt tagataatcc actgaattta tttgtctatg cgttaggtta cgcagtaggt
    13021 atcagtgtcg gaattttgat tgaagacaaa ttggcattag gatacatcat ggtcacaacg
    13081 attttgccag caaatagcgc agaagataaa agtttgccgg gtatactgag agaacacggt
    13141 tatggtgtga cacagacttc tgcaatgggg ctggaaggtg agcggctagt tctcgaaata
    13201 ctttctccta gaaaaagcga acgtgaactg tatcgcctga tcaaagaaat cgaagagcgt
    13261 gcttttatca tttcttatga gccaaaatat atttcaggtg gtttctggac taaaaaagtg
    13321 agaaaacgac aaggaaaata actcacaaac ttaaaaactg tctaatatct aaattaaatt
    13381 ttaaatgtta gacagttttt atttattatt ttgattaaaa aacatcgaca tatactcatc
    13441 aatttgttat tatactttat atacttatca atttaatttg gagatattat ggaaattcaa
    13501 cgtagaacca ctaatttttt acatttaggc tctttttttg caactttgac gatcttattt
    13561 tttctatgtt attcatacct taagacctca catgcgcctt ctagtgcatt aggctggttg
    13621 cttttggatc attctgatat cacagaagcc gaagaaaatc cgtttttttt gattttgtct
    13681 tctctttgcg gcttactgtt ttctgtttat ttcggtgcct atttttttca tcgacatcct
    13741 ggtacgttaa acgaagcttc aagcgtattg attgcattta aaacgttttc ttttttagca
    13801 attctttttt attcgtttaa tcacaatacg cttttcttct ttatcatgaa tggtttagtc
    13861 gtgattttat ccatttttac ttattttatt ttttcactca tttttagcca attgaaatgt
    13921 ccgctcttct ttcgattagt tcctgtcacc tgttttattt acagcttgtt ttcctttttt
    13981 gtccttgctt taatgccact gaccaaccct tcgacgatcc aagtcgcagg aaatctgttg
    14041 gatatgctct tcatcgtcag tctaggtgta tttatgtggc aaagaaaaaa caaacaaaat
    14101 aaaaagcatt ttgaagcctg actattacga cttcataaat gcttttttag ttttaaacac
    14161 ttcaatctaa aagaataacc caagattgga taaattatta atgactcgcc attcgtttct
    14221 tagtgattct ggattatcaa atggacttcc taatctaatc agctagttct tcccataaaa
    14281 tgccatactt gactgaatca taatattgat tatttaaaaa tctaactttt cttatcacta
    14341 cttcctttgt cattccacat ttctcaccat ttttttgcat tcctttgttt ccagaccatg
    14401 tagtgaatct aataggtggt agataggcaa aatactcaaa taattctttt aaccataaag
    14461 gaagagaaag gctcctcctg caagagtcag tataattttt gcttactgct ctcttaggtt
    14521 ttactcacta aaactgttcc atattctttg atataagaaa atgcatgcgg tataaatttc
    14581 ttgctcagct cagaaaagat ttgtcgttta agaaaggcca tttcctccat tgtcaatgaa
    14641 ggatccaatg ttggtaattc aatctcaaaa ttgttttttt cgacaatagg taattctaat
    14701 gtgaatgatt cttcttcctt tatagtattc gtcacaagta gagactgatg gaaaatacta
    14761 ggccggcgat ttttatattt attcttacgt atcctgtcaa cgataacttc tatttgatca
    14821 aaaaagtacg attcatttac gtctccaata agttcggcta attctttgaa tgccttcgtt
    14881 acaggaagct gattcttctg tacaaaaaag taattagcca aagtaagcgg gaagtgttct
    14941 atagaggaaa gtgtgataaa acggtacttc tcatttcctg cagttgtcct cagcaattct
    15001 gtctctcggc tgtttcttac tccataaaaa tacgcttctt tccgttcatc ttcgaaacaa
    15061 tacgcccaaa taacttctcc catcgctaat ttttgttcat caacagagag tctcttcaat
    15121 tgttcagcta tggtttcagc agccgaagtg gcttgttgca agtaatcttt aggatcaaat
    15181 attggaaagt tcaaatgata tcttcgttct tccctcttga ttaatcctgc atcgatcaat
    15241 aaatcaagaa aatgggaaaa tttattttgc gaaaattctt ttttcaattc tcttagaatc
    15301 gtttcttgtt ctcgatgttc atatagataa tcggtcaatg aagaaaaaag cggatcatcg
    15361 gtcagttttt gataaaagtt ttccttattt tttgaataat agttcatata tgttctcttt
    15421 tctatccgtt ttattttact taaaaggctt ttacgtaaaa aggaggaaga acaagagtca
    15481 taattgactg ttattctcct ccatttcttc ggcctttagt atgttctatg tttcttttta
    15541 tatcaatcgc ttatccattc attttgaatg cttcatacta ccatttgatt agctaaatac
    15601 agccgtcaat tgaggtacga cttgtttttt acgtgaaacg acacctttaa gcaaagcacg
    15661 gttgttggtc aaagttgttt ggaaagcttc ttctactttt tcttttcctt taccagcaac
    15721 aagtaattct gaatcactgt ctaagatgtt tgtgacaact aatacgaaca gatcgtagcc
    15781 atttgctttc atttcatctt ccattgctgc ttctagctct gcttgtcgat ccatcacttc
    15841 attaaggtcc actgtattga cttgagcgat acgaacgttg gaactgccca ttgggaaact
    15901 ttttgcatcc aaatcaagaa gaaccgcagc tgatttatca cttagatttg ttcctgcttt
    15961 caacatttca agtccataag tgtttagatc gacttcagcg attgctgcaa gttcttttgc
    16021 agctgcttca tcttcaggcg tacatgttgg tgatttgaac aacaatgtat ctgaaatgat
    16081 agctgagagc atgattcctg cgatttcttt aggaattgtt acttggtttt ctttatatag
    16141 ttttaaaata atcgtacttg tacatccaac tggttctgca cgatagtaca atggattagc
    16201 tgtttcaaag tttgcaatac gatggtggtc tactacagct aaaatattta cttcgctgat
    16261 atctgaaata ctttgttgga attcattatg atccacaagc atgacatctg cattttctgc
    16321 tttagctgac tctactactc gtggtgcaga tagctgaaag tggtctaatg cgtattgtgt
    16381 ttcttcatta ggtgtaccta gagcaactgc ttctgtttct ttacctaatt cattttgcaa
    16441 gtgtgaaaac gcaatggctg ccccgattgc atctgtatct ggattttggt gaccaaaaac
    16501 taaaattttt gacatgtcat tcgctccttt gtgatttctc ctatttaata atagcaaaaa
    16561 aacggaaaga agcaatactt atcttctttc cgatgcgacc tatcgcgtta aatattttgt
    16621 atactcttct gtatgaagca atcgtttagc attttcgaca cgttcttttg taggtggttc
    16681 gatcccttct aatggatatt tcaaaccaag tgtttcccac ttgtattttc ccatcgtatg
    16741 ataaggaaga atctctactc tgtctacatt tgacaaggtt tgaataaagt tgtctagtcg
    16801 gatcaaatat tcatcataat cacttcgctc aggaactagt acatggcgaa tccaaaccgg
    16861 tttgttgatt tttgacaaat attgagccat ttctaaaata ttttcattgt cccaatgcgt
    16921 tagtttttta tgctcttcat tatcgatgtg tttgatgtca aataacagta aatctgtata
    16981 cttcatcaat tcttgaaaac gtgaaaagaa aggttcttca tacgtaaaag gttttccgca
    17041 tgtatccaat gtagtatgca tcttccgctc ttttgctttt ttaaatagtt cgatcaaaaa
    17101 atcgatctgc aatagaggtt ctccaccact aacagtaata ccacctttat ctccccagta
    17161 aggtctgaag cgttctgctt tatctaaaag ttcatctgct gtgtattctt tccctccgcc
    17221 aatattccaa gtatctggat tgtgacaaaa ttggcaacgc atgcggcatc cttgcataaa
    17281 cacgacaaag cgcagtccag gtccgtctac tgaaccgaaa gtttcgatag aatgaacata
    17341 gccgattgtt ttttcttcca tttaagttca catcttttct ttgatttcat tttaagtaca
    17401 agttagctgt cgaagcgaag ctttctgaac aaaatttttc gcaattgccg cttcgttatg
    17461 tgtaaaattt acagggacgg aacatacaag tcctgcccct gtaaatcgtt tctttcaaat
    17521 aagcatactg attattcgat tttgatgcga tggctattca acgtcctttc gacgctaagt
    17581 cgaaaaactt ttttcacgaa ctttttacat tctgtcgtgt gaagttctca tcaagacatc
    17641 taattgttgt tctcttgtca agtcacggaa tttaactgca taaccagata cccggatcgt
    17701 caagttcgga tatttcattg gattttccat tgcatcacgc aataaatcat tactgaatac
    17761 attgacattc aaatgataag ctccttttga gaagtatcca tctaagacat tacgcaagtt
    17821 ttcgattctt gtatcttgtt cttttccaag acctgaagga ttgattgttt gggtatttga
    17881 aattccatcc aatgcacagc cgtaattcat tttagctgtt gagttcaggc tagctaataa
    17941 cccactttgt tccgcattgt aacttggatt tgctcctggc gcaagtggtt ttccagcttc
    18001 acggccatca ggagtatttc ctgtattttt accgtaaaca acatttgaag taatcgtcaa
    18061 taatgaagtt gttggttttg agttgcggta agttttatga cgtttgattt gtcccatgaa
    18121 gtattccaag atccatgaaa ctaattcatc tgcacggtca tcatcatttc cgtatttagg
    18181 gaagtcgcca tccacttcaa aatcaactgc tattcctttt tcattacgga ttgcttttac
    18241 ttttgcatat ttgatcgctg acaagctgtc tgccgcatga gagatcccag caatacctgt
    18301 agcaaatgtg cgttgcaagt cagaatccat caaagccagc tgagctgctt cataagaata
    18361 tttatcatgc atataatgaa tgatgttcaa cgtattgaca tatagttctg ctaaccaatc
    18421 catcatattc atgaagttat gtttcacttc ttcaaagtct agtatttctg atgtgattgg
    18481 acgatattct ggtccaactt gggcgccagt cacttcatcg actccgccat tgatggcata
    18541 taatagtgtt ttagccaagt tagcacgagc gccaaagaat tgcatgtctt ttccgacaac
    18601 tgttcctgat acacagcaag caatcgcaca atcatcagaa ccccattgtt cacgaagctg
    18661 attatcattt tcgaattgaa tcgaagaact ttcgatagcg attctgctag catatgttct
    18721 gaatccttct ggaaggtgtt cagaatacaa aacagtcaag tttggttctg gtgaaggtcc
    18781 catatttgtt aatgtatgaa gcagacggaa atcattcttt gtaactaatg aacgaccatc
    18841 taatcccata cctgcaatgg ataaagtagc ccagataggg aatcctgaga ataattggtt
    18901 atattcaggt gtacgcgcga atttgaccat acgtaatttc attactaagt ggtcgatcaa
    18961 ttcttgtgct tctttttctg tgattactcc attatccaaa tcacgttgga tatagatatc
    19021 aaggaatgct gagatacgtc cgattgacat cgcagcacca ttttgtgatt tgattgctgc
    19081 taaatatccg aagtatagcc attggacagc ttcttttgca tttgcagctg gtttagaaat
    19141 atcatagcca tatttcgcag ccatttcttt catttgcgct aatgcacgat attgttcagt
    19201 gatttcttca cgtaaacgaa tcacttcttc actcattgta tgataaccag tcatatcatg
    19261 atcttttttc ttttgttcca tcaaatagtc gattccatac aatgccacac gacgatagtc
    19321 accgatgata cgtccgcgac cgtaagcgtc tggaagccct gtaatgattt tgtttttacg
    19381 agcagcacgc atttcagcag tataagcgtc aaatacacct tgattatgtg ttttgcgcca
    19441 gtcagtgaaa atatgtgtca attcatcgtt tggtgtatag ccattgcttt ttagcgcatt
    19501 gttagccata ttgattccgc cgaacggcat gaatgcttgt ttcaaaggtt tgtctgtctg
    19561 tagaccgacg attttttcca aatctttatc caaatagcct gcatcgtgtg aagtaatagt
    19621 tgatacgatt tctgtgtcca tatcatagac accgttacgc tcgaattgga tatcatttaa
    19681 cttttgcaat tcatcccata atttttctgt tgcttctgtt gggccttcca aaaagctgtc
    19741 atcaccgcga tattcagtgt agttattttg aataaagtct ctagtgtcta cgccttcttt
    19801 ccatttggta cctttaaatc cattccattg ctccattgta tggacctcct aaatttctgt
    19861 tttatgtaac agtttttacg tgattagtat aacacagcac cctatctttg caagggtttt
    19921 cttgtttttt atttaacaat ttttatgttt tatttacata tatttaaatc aacgttgaca
    19981 taccaacatt taagcattgt gaacactttc atcttttttt gcaaacgtat aaggatttat
    20041 taaaaatgaa tgaaaaagag acgccacctg tattaataca gatggcgtct cttcattaaa
    20101 atactacaac agttacgact cgatgaaaat ctcaggagca tcgataactt cgatgacttc
    20161 tccacctttt ttctcatcca tcgcaaatga tccattcgat gttcgatcac ctatagggaa
    20221 atcagttggg tcaatcgtta actgctgccc tttttgattc atcaaggtta tttcttttga
    20281 tcttccttcc gacatataga taatacggtg cggatttttt ttcagctcac gcatgaccgt
    20341 cactccccgc ttcgcacgtg aagtttggct gatttcttga gcaagcatgc gtttaacagc
    20401 tcctcgctga gtaacgatta cgatcggcgt atcaccttct gctaacacaa gcagcccgtt
    20461 gataatccta tctccatctt tcaaattcat agacttcact ccagctgcct ttgctccaac
    20521 aacgggaaca tctgttaaag gatagcgcaa acccattcct tgatgcgtta ctaagaaaac
    20581 atctaagcct ggctgatctt ctttcagata cacagcaatc agttcatctt cttccgcctt
    20641 tagcttcata cagctgagcg gacggctctt ataggtgcgc catggttcga actctgtcat
    20701 tcgtgtttgc ttgatcaatc cattcttgct gatgaataca aatgttttct ctgcttgaat
    20761 tttttgatat gggaaaacag ctaaaatcga ttcgtctaca gacagattgg agattgtttg
    20821 agaaatgtgc tcaccagcat ctttccattt caaatcgggt agttcatgga cagggcggta
    20881 gatcacattt gctttatttg tgatcagaag aacatggtcc aatgtactca attcacctga
    20941 gtatagcaag tagtcgcctt ctcgcatccc gatttcttct ggtttggaag cactataaga
    21001 acgaaggctt gttcgcttca catagccttc tcgcgtcaca ctgacaatta cgtcttcttg
    21061 tgccactaag acttgcgtat caattttgat ttcttcgatt tcgtcttcga ttgtcgttag
    21121 acgagcggaa gaataatttt ttttcacttc tcgcaattct tttttcataa cagagaaaag
    21181 ctctttgtca tttgaaagga ttttattcaa ctctgtgatt tgcgcaatca agctttcgga
    21241 ttccttttga agctctgtga tatccgtgtt cgtcaaacgg tataattgca atgtgacaat
    21301 tgcttctgct tgttcttctg taaattgaaa gacgtcgact aaattctttt tggcgtcttt
    21361 tttgtcttta ctttcacgaa tcgttgcgat tacttcatcc agaatcgata aagctttcat
    21421 caatccttct acgatatgct gtcttttttg agctttggct aaatcgaatt gactacgttt
    21481 tgttatgact tgctttcgat gctcgatata acttcttaaa atgtctttca gtccaacttg
    21541 atgcggagtc atatggtcga tagcgaccat attgaaattg taattgatct gtagctcagt
    21601 gtttttgaac aaataattca aaattccttg agcattggcg tcttttttca attcgacgac
    21661 aatttgcaga cctgttctat cactttcatc tcgtacttca gctattccat cgattttctt
    21721 attcaaacgg atctcatcca tttttttcac caaggtcgct ttatttactt catatgggat
    21781 ttctgtgatc acgatttgct gtttattacc cttcattgcc tctatctttg ttttcgaacg
    21841 aagaataact tttcctcgtc ctgtttcata agcttttttg atttcatctt ttccttgcaa
    21901 gatccctcct gttggaaagt caggtccagg aatatattcc atcaattttt caaggctcgc
    21961 ttgtggatga tcgatcatat agattgtgcc atcgatcact tcagctaagt tatgagtcgg
    22021 gatttctgtt gcatatccag cagatatacc tgtcgatcca ttgacgagaa ggtttgggta
    22081 tcgagctgga aggactgttg gttctttttc tgtatcatca aaattccaca cgagatcgac
    22141 tgtttctttc tcgatatctg ctagcatctc tccacttaac ttggataatc tggcttcagt
    22201 ataacgcatc gcagcaggtg gatcaccgtc catactcccg ttattcccgt gcatttcgat
    22261 cagtacttcc cgcaacttcc agtcttggct catgcggacc atcgcttcat aaatactgat
    22321 gtcaccatga ggatgataat tccccatgat atttccgaca gattttgctg atttacggaa
    22381 tcctttgtca tacgtattgc catctttgtt catggcaaat aatatccggc gttgcactgg
    22441 cttcaatcca tcacggatat caggcagtgc acgttcttgg ataatatatt tggaataacg
    22501 tccgaagcgg tcacccatca cttcttctaa agttaattct tgtacttctt gtcgattttc
    22561 catcaggtga cctcctattc tacttcaaat aactgattgt tttcattttt atctgctcgt
    22621 tcttcatcta ataaatcatt agaaactgaa ggagagattt cagtatcctc ttttttatct
    22681 aagatactgc catcttcttc gagcgtaaat tgcacgtgat tttcgatcca ttttcttcga
    22741 ggttctactt tgtcacccat cagtgtggtt actcgacgtt cagcttgcgc agcatcatcg
    22801 atacggacgc ggatcaacgt acgggaagtc ggatccatcg ttgtttccca caattgttcc
    22861 gcattcatct ctccaagtcc tttatatcgc tgcagcatat atcctttgcc gactttttta
    22921 atcattgctg ccaattcgtc atctgtccac gcatattcaa tgacttgctt ctttccttgt
    22981 cctttggata ctttatataa aggcggtaac gcgatatata ctttcccagc ttcaatcagc
    23041 ggtttcatat aacgataaaa gaaagtaagc agtaatactt gaatatgggc gccgtctgta
    23101 tccgcatcgg tcatgatgat tactttgtcg taattacagt cttcgataga aaactcagga
    23161 ccgactcctg caccaatcgt atagatcatc gtattgattt cttcattttt caagatatct
    23221 tgcattttag ctttttcggt attgatgact tttcctcgta aaggtagaat tgcttggaac
    23281 tttctgtctc ttccttgttt agcagatccg ccagcagaat ccccttcgac taaatacaat
    23341 tcatttttct tagggtttcg ggattgtgcg ggtgtcaatt ttcctgaaag aagagattcg
    23401 ccttttttac gcttcttccc atttcggctt tcttctctgg ctttacgtgc tgcttctctc
    23461 gcttctctgg ctttgattgc tttacggacc aacatctggc tcatttcgct attttcctgc
    23521 aaataaaagc ccatttgttc actgatgacg ttatctacta cggtacgagc aactggcgta
    23581 ccaagttttt cttttgtttg cccttcaaat tgcagaagat tttcaggtac acggatagaa
    23641 agtacagcag ctaacccttc tctgaaatca ctaccttcca gattcttgtc ccgttctttc
    23701 agcaaaccaa cttttctcgc atattcatta taagatttcg tcatcgccgc tttcatacca
    23761 gcttcatggg ttcccccatc ttttgtccga acgttattga caaaagataa aacattttct
    23821 gaataaccgt cattgtattg ataagcgacc tctacttcga taccttcttt ttctcctgaa
    23881 aaataaacaa ctggtgtcaa tgtatctttt tcttcattca gataatcaac aaattctttg
    23941 attccttctt cgtaatgaaa aatttccttg actggttctt ctccacgaag atcagaaaga
    24001 gagattttaa cgccttttag aagaaaggca gattctctta accgttcggc taaaatttca
    24061 tatgaaaatt tcgttgtact gaatattgta tcatctggta aaaaatggac agtcgtcccg
    24121 tttgcttttt ttgtttttcc tatctttttc aaagtaccgt caggtttacc gccgttttta
    24181 aatttctgct gatactctac gccatctcta acgatcgtta caattagcca tttcgataag
    24241 gcatttacca cactggctcc tacaccgtgc agtccaccag aagttttgta ccctccttga
    24301 ccaaactttc cgccagcatg cagcacagta aaaatcactt caactgttgg tattccggaa
    24361 gcatgcattc ctacaggcat cccacgtccg ctatctgcaa cagttatact attgtcttca
    24421 tggatggtca catcaatctc actgccataa cctgataatg cttcgtcaac tgcattatcg
    24481 acgatttcat agactaaatg atgcaagcct cggctgtcag tggatccaat gtacatccct
    24541 ggtctttttc taactgcctc taatccttcc aatacttgga tggacgagtc attgtattcg
    24601 ttgtttactt tttttgccaa ggcaaacgct ccttcaataa tactttgcgg caaaaccgca
    24661 ccacgattat catactagaa aaggtcgtga aaaaaaagag ggtagacgag aaatcaattt
    24721 taattatctc ctaaccgctt tttcgacatt cccctctatc gatcatctct tctttgtggt
    24781 catttttatt tttggttttc tttcaactta ccaagttcaa tcttaataca acgatccatt
    24841 acgatatctg tacgtcctgc ttttcttagc atctcagctg cttctttgtt ttcaatccct
    24901 aattgtgccc agaacacttt agcatcagtt ttcaaaaaat cacctgctat ttcaggtaaa
    24961 aattcactcc gtctaaatac atctacgata tcgattggat catcgatttc tgtcaaattc
    25021 gcatagactt tctcgcctag aatttcttca ccagccaata atggatttac tggtatgatt
    25081 ttatatccat tgtcttgcaa tacctgtgca accatgtaac ttgttcgttc tggtttattg
    25141 ctcaagccta cgacggcaat attttttgct tcttccaaat agttgaaaat cgtttcttgt
    25201 gttggatttt ttacagacat ttctgccacc ccttgctttt ctttttattc catctattat
    25261 acttgtatct ttcatttttt caattgttcc tgttacctat tcatgctaaa ataaaggata
    25321 gttttcaact tgaaagaggg taaaaaatga aattgatctt attacttatt ttagcatatc
    25381 ttttaggttc tattccttcc ggtgtctgga ttggcaaatt gtttttcaaa aaagatatcc
    25441 gacaacacgg gagcggtaat actggaacga ccaatacatt tcgtgtatta ggcaaaacag
    25501 ccgggattgt tgtcctgctt atggatattt taaaaggtac attggctact tgtttgccga
    25561 tgatcttcca cctttctggg attaaccctc tttggtttgg ggtatgcgcc attcttggcc
    25621 atacatttcc gatttttgct gggttcaaag gaggaaaagc tgttgcgaca agtgcaggaa
    25681 tgcttttagg atttaatcct gtattttttg tttattcttg tatcatcttt atcgttagtt
    25741 tattttgtac aagtatggtg agtttgagca gtatgatcag tgcagtattg atcactttat
    25801 ctacgatcgt ccttccttat gtagctcctg tgattttggc taagcccaat tggttactga
    25861 cgatcattgc ctttttagtt tcttccttta tcttttatcg gcataaagac aatatcaagc
    25921 gtatcagaaa ccatactgaa agtcgaatcc cgtttggttt gaatagcagc aagaaaaaat
    25981 cctgatactt tttcattcca aagagataaa acgggagtgt gacaaatcga tgtcacactc
    26041 ccttattact tacaggatag cttttttaca caccttggta aacggtgttc cctgctaact
    26101 tctcggtcac aacctcttac ttcactgtga tggaatattt tgtcttgaag atttcatggg
    26161 cacctagttg attgatccct actttttcca tcaagtttcc actactgttc accgtgtcag
    26221 cgattccgca ccacggttcg atacaaacga aaggcgcttc ttttggataa ggagaccaga
    26281 tccctacata aggcatatct ttatagctta aagttacgct atgggaagat ttttcacttc
    26341 gaatggcgta cgcatttaga ccccttgttt caaaaaccaa ggcatcttgc tcaaaaagtt
    26401 cacgattcaa agccagattc gtatttgtct gacctaacgt cttttgatcc atatcgatga
    26461 atgggccttt caatggtagg cgtaatcttg atttcaatgg agaaaatgcc agatagaaat
    26521 cttcaaatga caatccttct tcaagaggta cattgaaagc aggatgtcca ccgatagaaa
    26581 agaacatttc tttttcaccg gtatttgcca cttgataatg aaccgtgatg ccgtctcctc
    26641 ctaattcata gctgatcact aattcaaaat caaatggata cttttctaat gtctccggcg
    26701 tactccttaa gatgaaacta gccatttctt gtccatgttc aatgacttca aaatccatat
    26761 cccgagcaaa tccgtgttgc cccatttgat atgttttccc ttgataaaca tactgatcat
    26821 cttttaaacg tccaacaaaa gggaataaaa ccggtgcatg tctgccccaa tacgctggat
    26881 caccttgcca gatatactct aaaccatgtt cttttgattt cagactgatc aattccgcac
    26941 cgtgttcagc gatttcggcg atcagctggt cattttgaat aataacagtc attgccattc
    27001 ctccaacttc gacttacttt ttaaaatggg aaggctggaa caaacatttc tatctgcccc
    27061 agtcccctta cttctaataa ctgtgttttg ctaactcgtc tttaaaacgg gaattcaata
    27121 cttttaaata agttcccttc atgcctaacg aacgagattc aatgattcca gctgattcca
    27181 attttcgtaa agcattgacg atcacagaac gagtgatgcc gatactatcg gcaatattag
    27241 aagcagtcag acgaccttca tctccatcca atgcttcaaa gatagcttgt acagccttca
    27301 attcgctata agataatgtg ttgattgcca tttggacagc cgttgcactc cgtacatctg
    27361 cttcgatgct tcgagacttc tgataaagga tctgcatacc gacaaccgta gctccatatt
    27421 ctgctaagac tagatcgtca tctgcaaacg attgttccat acgagataag ataatcgtac
    27481 ccaaacgttc gccagctcca aagatcggaa cgacagtcgt cagaccaaat ggatatttct
    27541 ctcttgactc taccggaaat gcagtaatat cactagtaat tgggatatta gcttcagttt
    27601 tcatcaactg tgccactgta tcagtataag attcaggaaa ttgtttctca acaaacattg
    27661 ttttgattcg ttcattgttc acatcgtgac gttcattata acctagtaat actccgtcac
    27721 ttcctatgat ataggtgttg ctgtcaagaa taccacctag tgtgacagcc attttatcgt
    27781 aaggcaaatc tgcttgcatg tcaaaagtat tcttttgctg caaaagtgtg tttatcttgc
    27841 gggttttcgt tagcaagtct gtcattgtcg tcctccgatt ttttataaaa tatagcgact
    27901 taaatcttca tttgccgcaa ttgagccaag tttgtcatcc acatatgctt ctgtgatcgt
    27961 tatttctccc atttgcatat ctggtgcttc aaataataga tcttctagta gtttttccaa
    28021 aatggtatgc agtctgcgtg cgccgatatt atctgtgtca cggttgacat cgaaagcgat
    28081 ttgagcgatc cgttcaattg cttcttttgt aaacgtaact gacacatttt ctgtaccgat
    28141 caacgcgacg tattgtttta ctaaagcatt atttggttcg gtcagaatct tcacaaaatc
    28201 ttcagctgtc aaatcatcta attctacccg tatcgggaag cgtccttgaa gttctggaat
    28261 tagatcactt ggtttagaca aatggaaagc tcctgaagca atgaatagga cgtgatctgt
    28321 ttggatgatc ccgtattttg tgtttacttg tgaaccttcg acaattggca atatgtctct
    28381 ttggacgcct tctctggata cttctccaga ttgctgtgat ttagaagtga tcttatcgat
    28441 ttcatcgata aaaataattc cactttcttc cgctaatttg attgcctcac tatggatatc
    28501 agcatccttg actaattttg ctgattcttc cttgatcaat aactctaaag cttctttgac
    28561 tgttaccgtc cgttccactt ttttcttcgg tgtcaaagca cctagagttt catttaggtc
    28621 gatccccatt tgctccaatc cattgttcat cattggtgca ggtttctttt ggtcttcaac
    28681 ttcaatggtt acttcacgac tatccagtaa acccttttgt agttgttcca agactgtacg
    28741 gcgattgatt cgaatatctt ctgtcacttc ttcttgcggt tcagcggtat tttgctgact
    28801 agcattaaaa agattcatca tttgttcaaa ttgattagtg gattgtttct gttctttttt
    28861 gataccaggt accaagattt ttaccaaacg tttttctgca ttttttttag cacttgtgta
    28921 tacacggctg tactgctctt tttctacgat ttggatcgca ttttctacta aatcccgaac
    28981 cattgattct acatctcttc cgacataacc gacttcagta aattttgttg cttcgacttt
    29041 gacaaatggt gcattgacga tttttgccaa acgtcgtgcg atttctgttt taccgacccc
    29101 tgtaggtccg atcatcagca tattttttgg tgtaatatct tgttgcattt tttcatctaa
    29161 ctgcaaacgg cgatagcggt tacgcaaagc aacggcaaca gatttttttg ctgcgtgctg
    29221 tcctacgata tattcatcta attcacttac gatttgtctt ggtgtttgtt ttaattcata
    29281 catatctact cacctcttac agttcttcta cgataatatt atgattagta aagatacaga
    29341 tatctgcggc aatatttaac gcattttctg caatttcttt cgcactcatg tcgttttgag
    29401 catagttttt cattgctcgg gctgcagcca acgcaaaatt ccctcctgat ccgatagcta
    29461 agataccatc atctggtgtg ataacttctc ctgtacctga aacaagaagc atttctttat
    29521 cattcattac gatcagcatc gcttcaagtt tttgcataga ttgctgtgtc cgccattctt
    29581 gagctaattc gacagctgcg cgttgtagat tcccgttata ttcgtttagt ttgccttcaa
    29641 atttttcttc taaagtaaac gcatctgcaa cacttccggc gaaacctaca acaacttctc
    29701 cattgtagat acgtcgtact ttctttgcag tgcctttcat gacaacagat tcacccattg
    29761 ttacttggcc atctccagcc atagcaaatt tcccgtcttt ttcgacggca cagatagtgg
    29821 ttgaatgaaa tggtgattcg accatgtttg tttcctccta aatttgttct tttccttatt
    29881 tttacgctct tggatggaaa gaacgatagt ttttttgtaa actttctttt gttacatggg
    29941 catagatctg ggtagtcgat aaattggcat gtcctagcaa ttcttggact gtccgcaaat
    30001 ctgccccatt atttaataaa tgagtagcaa atgtatggcg cagcatgtga ggatggatat
    30061 cactatccaa actgcttttt ttgatcattt gattcaagac atattcaatt ccagtaggcg
    30121 tgattggttt tccatgatga ttcacaaaaa cgatatcatg ctcttcgtga tattttgtca
    30181 tcaatacctg gcggccttcc ctgaaatatt cttgcagagc atcttgagca aaagaaccta
    30241 gaggagcata tctctctttg ttccctttac catggatcaa catgacctct gaatcaaaat
    30301 caatgtctga taccgacaaa ctgctacact cacttaaacg gatgcctgac ccgtaaagga
    30361 cttccagtaa agcgcggttc ctcaaatcca aaggactatc tcctgctacg ctgtcaaaaa
    30421 gtgcttgcat ttcattttca taaaaaaaac gtggcagtct caaatttttc tttttgagat
    30481 gaacgtaaga aaatggattt tcttttatca cttcttcttt gagaagatac tgataaaacg
    30541 aacgtaggct ggctatcttg cgactgatag aatttctgct atagctagga cgatgatcag
    30601 ctaatgcact caaatagaca cgaaccgtaa aatattccgc cttcagcaga tcagcgtctc
    30661 cagattcctc caaaaaatgt ttgaaatgga gaatgtcttc ttcatatgct tctttcgttt
    30721 tttctgaata tcctctttcg acaatcaaat aacgcaaaaa tttttgttcc cacgtggctt
    30781 ctgtcatttt ctcaccttcc ttttgtccca gagaataatt tagcacagcg tttataaaaa
    30841 gacaaataaa ttgtcagaat ttaaccaata tttctaagtt tgttcacaat tggcgcaatt
    30901 cctcacattt tgtttgagaa attcttaaga aaaacgcaag aaaaagagcg agactatccg
    30961 tctcaccctc ttcatggagt ttccttactc atttgggatt gcagtttttt ctaattctgt
    31021 ttttattgcc tcattagctt ttaaagctcg ttgtgcaatt gcttcatatc ttgctttttt
    31081 gtctcgaatg cgctctggca gttcaggaaa cagaccatag ttggcattca tcggttggaa
    31141 atgttttcct tccgcatgag taatgtagta agccatactt ccaatagctg tttcttgtgg
    31201 aaactcgact ggttccattt cttttgctaa gcgtgcggcg ttgattccag caagtagtcc
    31261 gctcgctgca ctttcaacat agccttctac accagtcatc tgacctgcaa agaacagatc
    31321 ttcgcgtttc gttgattggt aagtcggttt cagcaattct ggcgaattca taaagctgtt
    31381 tcggtgcatc acaccatagc gaacgaattc agcattttct aacccaggaa tcatccggaa
    31441 aacacgtttt tgctcgcccc atttcaagtg agtctgaaag cccacgatgt tgtataaaga
    31501 agctgcagca ttgtcttgac gcagctggat aacagcataa ggacgtttgc cagttttagg
    31561 atcttccaat ccaactggtt tcataggtcc gaaaagcatc gttttgaatc ctcgctgtgc
    31621 cattacttca attggcatac atccttcaaa atatttttct ttctcaaaag atttaagttc
    31681 tgccacttcg gcattgatta gtgcctcatg gaaagctttg aattcctctt ctgtcatcgg
    31741 acagttcaag taagctgctt ctcctttgtc atagcgggac ttcaaatata ctttatccat
    31801 atcgatcgta gctttgtcta cgattggtgc agctgcatca tagaaataaa aaccatctga
    31861 accattgaat gctttgatgc tttctgctaa tggatgagaa gtcaaaggtc ctgttgctac
    31921 tacggtgatt ccttccggta tttcagtaat ttcttcgttt atcactgtta ctaatggatg
    31981 ctctttgact tttcgggtaa ttgtttcaga aaaagaatcg cggtcaacag ctaaggcacc
    32041 accagctggc actgctgttt catctgccga agtgattact accgaattca atcggcgcat
    32101 ttcttctttc aataacccta ctgcattagc caaactgttt cctcttaatg aattggaaca
    32161 tactagttca gcaaaattac ttgtgtgatg cgcctcagtc gatttgactg gacgcatttc
    32221 atataaacgg acagggactc ctgcttgtgc gatctgccaa gcagcttcac ttccggcaag
    32281 ccctgctcca acaacattta ccattttcaa tgaaagaacc tcttttctat tatttttgga
    32341 tgtcttcttc gtaatctcca tttacacaga ctacttgttt accgcctttg actttcttct
    32401 cgaccagata tccgtcacat tttggacaat ttctgcccac tggtttatcc caagaagtga
    32461 attcacattc tgggaaacgg gaacaaccat agaatagtct gtttttctta gattttcgtt
    32521 caatgacttg tccttcatga catactgggc aagtcacacc gatttctttt acgattggtt
    32581 tcgtattccg acaatcagga aaattactgc aagcataaaa cttcccatat ttccctaatt
    32641 ttatcaccat cggatgtcca cagacatcac aatcgaatcc tgctggttca tctttgattt
    32701 gaattttttc gattttttct tccgcattcg tcagttcttt ttcaaacggt ttatagaaac
    32761 gatcaaccac tttgacccac gcttccgtgc cttcttcaat tttatccaaa tctccttcca
    32821 tttctgctgt gaaatggata tcgacgattt gagggaagaa ttcacagatt aatgaattga
    32881 caatttctcc tagttcagtc ggctcaaatc ttttttgtgt caattttaca tagtagcgtc
    32941 tttggatcgt atcaagtgtt ggtgcataag tggaaggtcg ccctaccccg ttttcttcta
    33001 gtgcacgaat caacgtagcc tcactaaaac gtgctggtgg ttgagtaaaa tgttgttttg
    33061 gttcaatatc aacagattgg actacatcgc cttcttcaag ttctggaaga atattttctt
    33121 tctcttcttt gccgtcatct ctaccttcca catacacttg catgaatcct ttgaatttga
    33181 tttttgaacc gttcgcaatg aagatgacac cattttgttc taatgtcact ttcattgtat
    33241 ctaaaatagc tggtgtcatc tgactagcca caaaacgtga ccagatcaat gtataaagtt
    33301 tcaattgatc tttatctaaa tattgtttga gttcatctgg tgtacgaagc acactcgttg
    33361 gacgcacagc ttcatgggca tcttgtgcgc cttgagcatt tttgatcttt cggtgatcat
    33421 gtctagaaaa ttcttttcca tacgtatctt caataaatgc tgcggcctct gcttttgctg
    33481 tatcggcaat ccgggtagaa tcagtacgca tgtatgtaat caagccgact gttccttgtt
    33541 tacccaaagt gattccttca tataattgct gtgctaccat catggttttt cttgttcgaa
    33601 agtttaactt acgggcagct tcttgttgca ggctacttgt agtgaaagga ttcgcagggt
    33661 ttcttttgcg ctctttcttt tctacttttt taacttcgaa gtctgttcca tcaattctag
    33721 cagtgacttc ttttacactt tgtgcatctg gtaatttctt tttcttaccg tcgattcccc
    33781 agaaatttgc tttgaatttc ttgcgagctt ttttaaaatt accgtcgatg ctccaatatt
    33841 cttctggaac aaattctcgg atctcttttt cacggtcaat aataattttt aaagcgacag
    33901 attggacacg tcctgcactt aaccctttct tgaccttacg ccagagaata ggactgatcg
    33961 aataaccaac gattctgtct aaggtacgac gagcttgctg tgcatctact aaatctacat
    34021 cgatcgttct tggttcctta aaagctgctt tgactgcgtc ttttgtgatt tcattgaaga
    34081 ccacccgatt tttttctttc agatccaacc caagtaggta cgctaaatgc caagcaatcg
    34141 cttctccttc tctatccggg tcacttgcca agtaaacttt ttgagctttt tttgctgcgg
    34201 ctttcaggct tttgatgaca tcgccttttc cgcgtataga aatataatgg ggttcataat
    34261 tgttttcaaa atcgataccc attttacttt tcggtaaatc tcttatatgg ccaacactgg
    34321 ccattacttt atagtttcgt ccaagatatt tctcaattgt cttggctttt gcaggtgatt
    34381 ctacaatcac taaatattta tacgccatta ctgaatggac tcctttccat tgtcgtgttc
    34441 ctactattaa aagaggattc atacaaatcg tagctaatca tatccattca gaaaacgttt
    34501 gtcaagaaaa gcaaatgaaa agtatggtgt tattctttaa attctgccaa gatatcttca
    34561 cccgaaaccg tgcaaactgc accgtcttgg atcaaatgat gacatccggt agaatgttct
    34621 gttaaaatat ttcctggaac agaaaaaact gttcttccaa gatccaatgc ttgctgtgct
    34681 gttatcagag agccgctttt tttcttggct tcaaagacac atgttccttt agacaaagct
    34741 gcgataatcc gatttctcat tggaaaacaa tgctttttcg ggccggattg aggaggatat
    34801 tcactcaaaa gaagatgatc atttgcaatc acctcttgca agtcacgatt ttcagcagga
    34861 tagacttgct ctatccctgt tccaatgact gcgattgtcc gtccaccatg agagattgct
    34921 gcttggtgac cgtatgtatc tatccctttc gcgcagccac tgacgatcac atatccagct
    34981 tctatcactg gttttagtaa ataataaact gtttttttgg ctaaagcaga agtgtgtctt
    35041 ccaccaacaa cgcttaacat gttacaagaa agtaaagata gatcaccatt ataaaacagt
    35101 acaattggcg gataggtcaa gtgaagcagc tgagggggat atttgggatc aaagcaagtg
    35161 ataaacgact gtttcgattg gattttgccc cagttagcgt gaatttcctg ccaacttttg
    35221 cgaaacagct cccggtgttt ggttatttta cctatcttaa tgatgtcttc aaatccgatt
    35281 tctttataag gatactgcat catccagtta gcaagtgccc attttccttg actactgatt
    35341 ccttttgtat aagcaagttt caataaattt tcttctattt gatacatcat aaaaccgcct
    35401 ttcgtctctc tttctaataa gatacgtaaa agcggctgtt ttttattgta aatatttttt
    35461 tacaggtgca aaacttttac gatggattgg cgtaattcct tgtcgttcaa gtccttctaa
    35521 gtgttccttt gttccataac ctgcattatg cgagaaacca tatccaggat aaagcacgtc
    35581 atattctttc atcaattggt ctcgatatac tttagcaatg atacttgcag cagcaatcga
    35641 cacagatcgt gcatcccctt tgattatttt ttcctgctga ataggcaaat ccaaggtcat
    35701 cgcatcgatc aacaaatagc ttggtacaat ggacaatttt tctactgctt ggatcatcgc
    35761 taatttactt gcttcataaa tattcacatc atcgattgtc tgctgatcaa cgatcccgat
    35821 cccgattgcc agggcttttt cttgaacttg ttgataaaga agttctcgct ttttctctga
    35881 taattgtttg gagtcattca gccctaatat gatctcattt ttcggcaaga taactgcagc
    35941 tgccacgaca ggtccagcta atggtcctct tcctacttca tcaatgccgg caatccattg
    36001 gtgaccttgt gcgtaccctt gcttttcaaa gcaaaacatt tcttcatatt tttccttaat
    36061 taatgcttcc tttttcaatc gtcgttcaaa agaagccagt gcttgctgca cgcctttgcg
    36121 tggatcattt cttaatgatt ctagtgtagg atcatctatt ctctgtatgc ttttcaatcg
    36181 ttctttgata ctttggatgg attcagtcat cacttttttg ctccatttct tcgcaacgat
    36241 ccagtgtgta tcgtccaagt tttccttgac gaatatcatg cacgactaat tcgctgccac
    36301 gatcatagtc ttctttatac ccacgttttg ctgtaatatc catcagtaat tccggtgcca
    36361 atttttcttc ttgctcgatt gataaaccat aacgatcgct gattcttcct gaataatatc
    36421 gggcaaaaaa gtctaatccg taaatcgcta aatcatctaa atggacaagc tggtctttga
    36481 tcgcgcctgt cagcgctagt tttttgccga tttcttcatc ttcaaatttg ggccataaaa
    36541 tccctggtgt gtctaacaat tccaactcat tacctaaacg aagccactgc tgaccttttg
    36601 ttacgccagg cttatttcct gtctgtgcaa ttttcttgcc tactagtcga ttcatcaacg
    36661 tagatttccc aacatttgga atacctaaga ccattgcccg aatagcgcga ggcttgatcc
    36721 cccgagcagc atctcttgcc cgtttttctt tcaatgcttc tttagccgca ggtactagac
    36781 ctttaattcc tttactttct tgtgccgtga tagctaatgc aagatggcct tgtttacgaa
    36841 aatattcttg ccacagcttt gtctgttgtg gatctgccag atccgcttta ttcaataaaa
    36901 caaggcgagg tttttgctga atgatctgat ctagcattgg attacgagaa gataaaggta
    36961 atcgtgcgtc taccaattca aatacgatgt ccacatattt cattttttca gaaacttctc
    37021 gtcgggcttt tgccatatgt cccggaaacc attggatggt cattgttctc tccattcctt
    37081 tcataccgga atattccccg gctttacttg tttttttctt tttcaatata aattcacttt
    37141 tttattataa agaaattcca tctattagtt aagtgaaagt gaaccagtcc tcattctatc
    37201 acatcttcac ttttttgatg atgcaaattt ttattttggt ttctatatat gaatctaaat
    37261 ttttgacagc gtttcctaaa taagagatga tagacgaaca aaacagaaag gagacgaaac
    37321 aaatgaatag tatggttttt gttaattttc ctgttgctga tgtcaatcgt tccgttgctt
    37381 tttatgaaaa gctgggtttt aaaaaaaatg aagaattttc tactgaagaa gctggttcaa
    37441 tggtatggga tgacactttt tggataatgc ttctctctca tgatttttat aaaaaattta
    37501 taaaagataa agaaatagct gataataaaa aaacaagtgg tgctttgatt tcgtttagtg
    37561 ttgaaaatgc ggatgaagta aaaaaatttg ctcaaattgc aaaagaaaat ggtggtactt
    37621 tccacgaagt agatatggga atgcctgaag aagaaatgtt ttcattagaa gtccaagatc
    37681 tagacggaaa tacattagaa cccgtatgga tgaagatgta agcagataac taatctgacg
    37741 ttttttataa caaaaaaaca aaggcggatg atacgataag tattctccgc ctctattttt
    37801 tttattcgta caatctcttt tttacagttt ataaaagcag tcttctcacc ctttttttaa
    37861 ttgttagatg ccagtgtatg taaatcacca atgtgtcatg acacattggt gattttatgg
    37921 tatcctttta ttattaactg acaaaggaga gaatattttg aacactttga ttgcaaaaga
    37981 cgtttttaga ttgcccgaaa tgaatgcaag attgttgtcg acctctgctc ttttattagc
    38041 ccttgatgtc atcttataca agatagcgat tggtcctgct tattttcaaa tcacattcgg
    38101 atttttaagt atggcgctta tgggttatct atatggaccg atctgggctg gtttgcttga
    38161 aatgttgtcg aacactttaa atttcacgat atttggttca ggaacgtttc aagctgcttt
    38221 tcttatcacg gctttccttg gtggatttat taatggctta tttttatatc ataaaaagat
    38281 aaacctgcct tccgtcatcg ctgcccaatt attgattatg atcccagtca gcctttttat
    38341 caattcttgg ctgatgtctg ttttgtttgg tgcagatttc aaagtaattt ttctaggtag
    38401 attcatcagg aatatgatcc aaatccctat tcaaataatg gttgtttatt catttatcaa
    38461 aactttagag gctagaaaag tgtttaaacg agctgtttga ttttacgtac taaggtagta
    38521 aactgatttt ggattaaaca ataaagcgtg cagtctcaac agactgcacg ctttattgtt
    38581 tctcactgga tccattttat ttcatcaagt ggataataga caaatcttgc tttacctaga
    38641 atatcagttt tattgatcgt gccaaacgaa cgactatctt ttgaaactct tcgattatct
    38701 ccaagtacaa aataattatc ttccggaagc ttggattgtc ccgttagctc ttgtagtgta
    38761 aagtccgtcg tataggatgc atttgcatga tctgatttca ggtttttagt tagataaggt
    38821 tccttaacta ctttcccatt gatcttcaat tgatcatttt ggtagctgac attttctcct
    38881 ggaagaccga ttactcgttt gatataaatc gtgccatctg ctaattgaaa aacaacaata
    38941 tcaaatcgtc tgatctccga aaatttttcc atcacaacca tatcaccttg cttcaacacg
    39001 ttttccattg aattgccttc cactggtacc gggatgaata aaaatccccg caacatgaac
    39061 gcaaggaata cagaaacaag taagtatttg aatacaagcc agaagcgatt aatatagcgt
    39121 tgtttttttg tcaatctcta tcaatccatc cctctttttt tcattctttc tttattataa
    39181 gaaaaatatc aaaaaaatgc gactagatag ctgaaataaa aataattctt tcaattaggc
    39241 tattggacaa aaaacttttt tgatcataaa gtacaaaact atctcttata tgtcatgatt
    39301 attttctaaa tatttttccc atgcttcgtc aaagatatcc atggacggca aatagtcgac
    39361 attcagttcc agataattag aaatttcttc gtagctagag ctgtgcttag gaaattggat
    39421 gtctttaccg gcttctgtag cgaaaacaga taaagcatca tgtgtgccac cgcctcgcag
    39481 tgtcattaaa taatgaaaaa agcttcgatt cacccttcgt tcctccaatt ttcttcgata
    39541 aaggcagccc ggcgtgcatg gtttctttta aaattttcag gatgcttttt ataatactct
    39601 tgatggtctt cttctgctag ataaaatggt tgggctggtt cgatttttgt aacgattggc
    39661 tgttcgaaac gtccactttc agctaactgt ttcttacttt tttcagcaat gatccgctgt
    39721 tcttctgtca aataaaagat caccgggcga taattgtcac cgcgatcctc gaattgtccg
    39781 aaagcgtcag ttggatcagt ttgctgccag taaagttcta ctaattgttc atatgaaatc
    39841 aatgttggat catagataat ctccactgct tctgtatgtc cagtcgtttg gctttttact
    39901 tgttcataag tcggatctgg tacatgccct ccagtatatc ctgacatcac tgtatggata
    39961 ccaggaagcg tgtcgaaagg ctggatcata caccagaagc agcctcctgc aaatattgct
    40021 cgttccatta tgattccccc tctaatacta ctcatccgat gattttttat tttcttttgt
    40081 cttgctttca gtactgctgg tgctagtttt gttggtattt gagttgtttg aagcatcaga
    40141 ggtatctttt ggcaaatata gactaaaccg aatgtcatca tttactaagt cgattttatc
    40201 tgctttaata tacatcccgt tttgcattcg gaattgatcc aacctaagca tgatagtcag
    40261 atcatctgta ttgacttcga tccaatctgg taatttatag tttctcttga tcatattcat
    40321 cacatctttg attggcaaac tcaatgttcc gatagaaaga cttctagctt ttagttggac
    40381 atttccgttt tccatcacat acggatcaaa atataaatag aaatcgatag gaaaacctag
    40441 tacttcgaaa gtaccattga gtagtgcttc attttccaaa taaaatttat attcaatatc
    40501 actatctttt tgatactcac taaggaaaaa gctgatgatt tgattgactt tttctttatt
    40561 tgaattgatc gttaagacag gatctccttg tctttccgta agagctggta tttctgggag
    40621 ttctggttcc cgattagcaa aaatacggct gcctaaaaat acggtcgaac caagtactac
    40681 cccaagaagg ataaggaagg cgaatttcca gtaatttgtt ttctttgttt tgtttgaaga
    40741 tacgtcagat cttgttttca tgtttattga tcatcctcct tctctaacca ttggttttgt
    40801 gtctcgatca gctgatctct cactgcattt gccatcaatt gataacctaa attatttgga
    40861 tggaaatgat cttcttcata gagcacatta tttttgatgc catttagatt ttctgtcgtg
    40921 ctagttgttt gatctgttcc ttcttctgtg acacctactt cctgattcaa tccttgataa
    40981 agcaaatcat tgattgggat aaagtagcag ttcggcattt tttttgtcac cgtttctgtt
    41041 cctttgttcc aattgttaac aattgtctgc atgtcagtga tctcaggaaa gttcaaataa
    41101 aatggattgt agatgcctaa aatgtaaata ggtgcggttt tattgaggga tcgaaattct
    41161 tccaatagtt ctgtgacatt ttcctgatag tttttcagtg gctttttaaa ggaattgacc
    41221 gttaacccga ataaattatt ttggataact ttcatcaaat cattaccgcc aacagtcagt
    41281 gtgataaaat cagctgtttt gatattgttt cgaatttctt cttttgtttt tactcgcttt
    41341 aagatctggt cacttcgttc accatttaca ccatagtttt ctgtttcaac cgcggtcaaa
    41401 ttataacggt cttttagatc atttgcgaca aggggaacga atcctccttg cttggtttga
    41461 tctcctactc cttcagtcag cgaatctccg atagctatgt agtgaagttt ttctttttta
    41521 tttggatctt ctctcacagt agttactggt ttcattagtg ggccagcttt cggtatagaa
    41581 gcagctaaac ctgcatagat gatgatcgca attaataatg ggaacaaaac gaggatcaaa
    41641 tgtcgtttcg ttaatttcat tttttcacct cttgtattcg attggttcta atgttatcaa
    41701 aaattttatc aaaaaggtag tctttatgaa aaaagaaaac aattattttc ctaattttat
    41761 aaagaaaaag agaataagct gcaagccttt caggctgagg gcttatcctc ttttcgacta
    41821 atctacataa tacatgattg caaacgcgtc tttaccagta tgcgtagcga taaccggatt
    41881 cgtatgcagt acagggatat gcatgtccgg gaataattct tgcaaaccct ctttgaattc
    41941 ttgcgccaat tcttttgcat ccgcatgtga aataccgatt tgtcgcacat ttggcaattc
    42001 tttcagttca tttttcaaat tattaaacca tttatcgaac gttttctttc ctcgcccctt
    42061 agttacaggg attaattcag aatgggcgaa attcatcacc acgcgtatgt tcaaaacact
    42121 agacagcaaa ccagttgttc ggctgattct tccacctttt accaagttat ctagtgtgga
    42181 tattccgatg tataattttg tattgtcttg tacttttttg acttcttcaa ggattttctc
    42241 tactgtttta ccttcttttg ctaattcagc tgctcgaatt acctgaaaag ataatccttg
    42301 gtcagtggta tcactgtcga taaccgttac tttcgttttt gttagattac tggcttgtct
    42361 tgctgcttct actgttccac tcaagccttt tgtcatatgg atcgaaatca cttcactgcc
    42421 gtcttctcct agctcatcat acatcgagat gaattctcca attggtggtt ggcttgtttt
    42481 gggcagattt tttgctttcg ccatcatttc cataaatctt tcgccgtcta gttggtcatc
    42541 atcgggatat actgttccgt cgatcatgat ggatagcggc atgacacgaa tgcctaaagc
    42601 atcgcgtaca tttttttcca tcgtacatga agaatctgtt acgattttta catttgtcat
    42661 tattttctct ctctctttct ttaacaagat gccttattca tgttaaacta catgtaagac
    42721 aatttctatt tttagtataa cagacaaata atgatttttc attaattaca cctagtaaag
    42781 gagccgtttt catggaaaaa acgaaattta atcgaacgta tgaaatatta aatgaagtgt
    42841 ttaatgcagt cacccatgga attgggactg gattgagtat cgcgggacta gtggttcttt
    42901 tagtaaaagg tgcccacttg cattcctccc tccatattgt ctcttatgct atctatggat
    42961 cgatgatgat tcttttattt ttaacgtcta ctttgtttca ttcattgatt tttacaaaag
    43021 caaaaaaggt gttccaagta ttcgatcata gttcgatttt tcttttgatt gcaggaagtt
    43081 atactccttt ttgtttgctg agtatccgag gatggctagg ttggacttta tttgtattaa
    43141 tctggttgtt tgcgattctt ggcattatat ataaagcgtt gacgttgcat aaacgggaga
    43201 gagtatcaaa agtatctacg atcatctata tttttatggg gtggctctgt gtcgtggctg
    43261 ctgttcctct ttatcactct cttggaatga ctggtgttat tttgatggta gcaggcgggc
    43321 tttcttacac gataggcgca ttcttttata gcttgaattc tatccgctat atgcatgttg
    43381 tttggcattt gtttgttatg ttaggtgcag gttttatgta cttctctgtt ttattcttta
    43441 cttaataaaa aaaataaagc tatggatggg acagctctaa ataacagcta gtctatccat
    43501 agctttttta tatcgtcaat tcgcctgtcc cgctttttac aggattctca ttacgctgat
    43561 acgtttcaaa ttgatgcgga taacgatttt tttcatccat ttctccttga ctagtattga
    43621 tcagtgacca atcagaccag tcaacaggtg gaaaataagc atctccatcg aatacatgat
    43681 ggatgactgt acggtataat atcgtacaaa atggcagaaa ttcttgataa acagctgacc
    43741 ctccagcgat gaaaaagata ccgtctgttt ctttttccag agcaagcacg tcttccacac
    43801 tatgcatgac atgcacgcct tctgctttat aggaagaatc tctcgtcaaa acaatcgtct
    43861 gacgattcgg caacggacgg ctacccatcc cttcgaatgt ttttcgtccc atcactagtg
    43921 tatttgcttc tgtcatttgt ttgaagaatt tcagatcatt tggcaaacgc caaggaagac
    43981 ggtcttcttt tccaatcaat ccgttttcat cctgtgccca aattgcagct aacattttct
    44041 catcctttct gattctaaac agcgatagga gctttgatcg ccggatgtgg gtcatatcct
    44101 tctaattgga tatcttccat ctcaaaatca aatactgact gcttttcctg attcaaaact
    44161 aaagtcggaa atgcgcgtat ttctcggctc aattgttctt tcatttgttc gatatgattg
    44221 atataaagat gagcatctcc taatgtgtga acaaattcgc caaccgctaa acctgtttca
    44281 tgtgcaatta ggtgagtcag caacgcataa cttgcaatgt tgaacggaac gcctagaaac
    44341 acatccccac tgcgttgata caattggcag cttagccgtc cctcttgaac ataaaattga
    44401 aacatcgtat gacaaggagg caaagccatg ctaggaacat cttccggatt ccatgcagaa
    44461 acgatcaatc ttctagaatc aggagtcttt ttgattgctt cgatcacttg tttgatttga
    44521 tcgataaagc caccgtcttt tgtttcccag tgacgccatt gatagccgta gatgtgtcca
    44581 agatcgccgt atgtttcagc aaaggaatca tcggttagga tcttctcgca aaaaagcttg
    44641 ctctgctttt tatattcttc attgaattca ggatctgtca aacatctatg gccaaaatca
    44701 gtcatatctg gtccattata gtctggactt ttgatgtagc gttcaaatgc ccattcatcc
    44761 caaatatggt ttttattttg taatagatag cggatattcg tatctccttt taaaaaccaa
    44821 agcaattcgc ttttgatcaa tccaaaaggt actcgtttcg ttgttaacaa aggaaaccct
    44881 ttttgcagat caaaccgcat ttggtatccg aataagctta atgtgcctgt gcctgttctg
    44941 tcttctttac gatgcccttc ttccaacaat tttcttccta agtcaagata cgcttgttcc
    45001 atcct
//
LOCUS       DAZBSA010000009        44831 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000009 DAZBSA010000000
VERSION     DAZBSA010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.009"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..604
                     /locus_tag="ACHGMT_000472"
     CDS             212..604
                     /locus_tag="ACHGMT_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFN6834244.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ACHGMT_000473"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ACHGMT_000473"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFN6834245.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2698..2943
                     /locus_tag="ACHGMT_000474"
     CDS             2698..2943
                     /locus_tag="ACHGMT_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834246.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3165..4775
                     /locus_tag="ACHGMT_000475"
     CDS             3165..4775
                     /locus_tag="ACHGMT_000475"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFN6834247.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4752..4964)
                     /locus_tag="ACHGMT_000476"
     CDS             complement(4752..4964)
                     /locus_tag="ACHGMT_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834248.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5067..5936
                     /locus_tag="ACHGMT_000477"
     CDS             5067..5936
                     /locus_tag="ACHGMT_000477"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFN6834249.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="ACHGMT_000478"
     CDS             6101..7372
                     /locus_tag="ACHGMT_000478"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFN6834250.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="ACHGMT_000479"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="ACHGMT_000479"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFN6834251.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="ACHGMT_000480"
     CDS             8763..9026
                     /locus_tag="ACHGMT_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFN6834252.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="ACHGMT_000481"
     CDS             9471..9920
                     /locus_tag="ACHGMT_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN6834253.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="ACHGMT_000482"
     CDS             9977..11395
                     /locus_tag="ACHGMT_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFN6834254.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="ACHGMT_000483"
     CDS             11395..12255
                     /locus_tag="ACHGMT_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFN6834255.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="ACHGMT_000484"
     CDS             12429..13202
                     /locus_tag="ACHGMT_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFN6834256.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="ACHGMT_000485"
     CDS             13349..13714
                     /locus_tag="ACHGMT_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFN6834257.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="ACHGMT_000486"
     CDS             complement(13862..15070)
                     /locus_tag="ACHGMT_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN6834258.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="ACHGMT_000487"
     CDS             complement(15089..15367)
                     /locus_tag="ACHGMT_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834259.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="ACHGMT_000488"
     CDS             15560..16216
                     /locus_tag="ACHGMT_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFN6834260.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="ACHGMT_000489"
     CDS             complement(16658..17455)
                     /locus_tag="ACHGMT_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFN6834261.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="ACHGMT_000490"
     CDS             complement(17452..18636)
                     /locus_tag="ACHGMT_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFN6834262.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="ACHGMT_000491"
     CDS             complement(18661..19908)
                     /locus_tag="ACHGMT_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFN6834263.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="ACHGMT_000492"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="ACHGMT_000492"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFN6834264.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="ACHGMT_000493"
     CDS             21888..22202
                     /locus_tag="ACHGMT_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFN6834265.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="ACHGMT_000494"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="ACHGMT_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFN6834266.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /gene="tmk"
                     /locus_tag="ACHGMT_000495"
     CDS             22887..23531
                     /gene="tmk"
                     /locus_tag="ACHGMT_000495"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFN6834267.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="ACHGMT_000496"
     CDS             23546..23875
                     /locus_tag="ACHGMT_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFN6834268.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="ACHGMT_000497"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="ACHGMT_000497"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFN6834269.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="ACHGMT_000498"
     CDS             24863..25687
                     /locus_tag="ACHGMT_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFN6834270.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="ACHGMT_000499"
     CDS             25680..26027
                     /locus_tag="ACHGMT_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFN6834271.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="ACHGMT_000500"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="ACHGMT_000500"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFN6834272.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="ACHGMT_000501"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="ACHGMT_000501"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFN6834273.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACHGMT_000502"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACHGMT_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFN6834274.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACHGMT_000503"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACHGMT_000503"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFN6834275.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="ACHGMT_000504"
     CDS             31684..32451
                     /locus_tag="ACHGMT_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6834276.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="ACHGMT_000505"
     CDS             32441..34483
                     /locus_tag="ACHGMT_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6834277.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="ACHGMT_000506"
     CDS             34764..34913
                     /locus_tag="ACHGMT_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFN6834278.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="ACHGMT_000507"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="ACHGMT_000507"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFN6834279.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="ACHGMT_000508"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="ACHGMT_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFN6834280.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="ACHGMT_000509"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="ACHGMT_000509"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFN6834281.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="ACHGMT_000510"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="ACHGMT_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFN6834282.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="ACHGMT_000511"
     CDS             39330..40412
                     /locus_tag="ACHGMT_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFN6834283.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="ACHGMT_000512"
     CDS             40412..41215
                     /locus_tag="ACHGMT_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFN6834284.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="ACHGMT_000513"
     CDS             41285..42025
                     /locus_tag="ACHGMT_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFN6834285.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="ACHGMT_000514"
     CDS             42036..43040
                     /locus_tag="ACHGMT_000514"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFN6834286.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44830)
                     /locus_tag="ACHGMT_000515"
     CDS             complement(43085..44830)
                     /locus_tag="ACHGMT_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834287.1"
                     /translation="MAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTI
                     PTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSL
                     AVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIA
                     VFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFL
                     SHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPI
                     ANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTIN
                     WSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNT
                     LRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQK
                     LAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHE
                     QEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFI
                     DRITVQLSRNYPNPV"
BASE COUNT        14406 a         7824 c         9339 g        13262 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg tgcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat t
//
LOCUS       DAZBSA010000010        44504 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000010 DAZBSA010000000
VERSION     DAZBSA010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.010"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1879)
                     /locus_tag="ACHGMT_000516"
     CDS             complement(<1..1879)
                     /locus_tag="ACHGMT_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFN6834288.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VE"
     gene            complement(2042..4120)
                     /locus_tag="ACHGMT_000517"
     CDS             complement(2042..4120)
                     /locus_tag="ACHGMT_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFN6834289.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(4133..4408)
                     /locus_tag="ACHGMT_000518"
     CDS             complement(4133..4408)
                     /locus_tag="ACHGMT_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834290.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(4770..5225)
                     /locus_tag="ACHGMT_000519"
     CDS             complement(4770..5225)
                     /locus_tag="ACHGMT_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834291.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(5379..6587)
                     /locus_tag="ACHGMT_000520"
     CDS             complement(5379..6587)
                     /locus_tag="ACHGMT_000520"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFN6834292.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(6699..7031)
                     /locus_tag="ACHGMT_000521"
     CDS             complement(6699..7031)
                     /locus_tag="ACHGMT_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFN6834293.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            7179..8054
                     /locus_tag="ACHGMT_000522"
     CDS             7179..8054
                     /locus_tag="ACHGMT_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN6834294.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(8136..8717)
                     /locus_tag="ACHGMT_000523"
     CDS             complement(8136..8717)
                     /locus_tag="ACHGMT_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFN6834295.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(8723..9982)
                     /locus_tag="ACHGMT_000524"
     CDS             complement(8723..9982)
                     /locus_tag="ACHGMT_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFN6834296.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(9984..11030)
                     /locus_tag="ACHGMT_000525"
     CDS             complement(9984..11030)
                     /locus_tag="ACHGMT_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834297.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(11265..11540)
                     /locus_tag="ACHGMT_000526"
     CDS             complement(11265..11540)
                     /locus_tag="ACHGMT_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFN6834298.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(11783..13093)
                     /gene="der"
                     /locus_tag="ACHGMT_000527"
     CDS             complement(11783..13093)
                     /gene="der"
                     /locus_tag="ACHGMT_000527"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFN6834299.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(13275..14504)
                     /gene="rpsA"
                     /locus_tag="ACHGMT_000528"
     CDS             complement(13275..14504)
                     /gene="rpsA"
                     /locus_tag="ACHGMT_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFN6834300.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(14639..15319)
                     /gene="cmk"
                     /locus_tag="ACHGMT_000529"
     CDS             complement(14639..15319)
                     /gene="cmk"
                     /locus_tag="ACHGMT_000529"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFN6834301.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(15368..16003)
                     /locus_tag="ACHGMT_000530"
     CDS             complement(15368..16003)
                     /locus_tag="ACHGMT_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFN6834302.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(16069..17472)
                     /locus_tag="ACHGMT_000531"
     CDS             complement(16069..17472)
                     /locus_tag="ACHGMT_000531"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFN6834303.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(17459..18490)
                     /locus_tag="ACHGMT_000532"
     CDS             complement(17459..18490)
                     /locus_tag="ACHGMT_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6834304.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            18521..18742
                     /locus_tag="ACHGMT_000533"
     CDS             18521..18742
                     /locus_tag="ACHGMT_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFN6834305.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(18907..19644)
                     /locus_tag="ACHGMT_000534"
     CDS             complement(18907..19644)
                     /locus_tag="ACHGMT_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFN6834306.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(19797..21137)
                     /locus_tag="ACHGMT_000535"
     CDS             complement(19797..21137)
                     /locus_tag="ACHGMT_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFN6834307.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(21374..22897)
                     /locus_tag="ACHGMT_000536"
     CDS             complement(21374..22897)
                     /locus_tag="ACHGMT_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFN6834308.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(23275..23886)
                     /locus_tag="ACHGMT_000537"
     CDS             complement(23275..23886)
                     /locus_tag="ACHGMT_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFN6834309.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(24009..24134)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(24216..24932)
                     /locus_tag="ACHGMT_000538"
     CDS             complement(24216..24932)
                     /locus_tag="ACHGMT_000538"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFN6834310.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(24938..25507)
                     /gene="scpB"
                     /locus_tag="ACHGMT_000539"
     CDS             complement(24938..25507)
                     /gene="scpB"
                     /locus_tag="ACHGMT_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFN6834311.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(25491..26261)
                     /locus_tag="ACHGMT_000540"
     CDS             complement(25491..26261)
                     /locus_tag="ACHGMT_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFN6834312.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(26258..26629)
                     /locus_tag="ACHGMT_000541"
     CDS             complement(26258..26629)
                     /locus_tag="ACHGMT_000541"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6834313.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(26648..27535)
                     /gene="xerD"
                     /locus_tag="ACHGMT_000542"
     CDS             complement(26648..27535)
                     /gene="xerD"
                     /locus_tag="ACHGMT_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFN6834314.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(27631..28104)
                     /locus_tag="ACHGMT_000543"
     CDS             complement(27631..28104)
                     /locus_tag="ACHGMT_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFN6834315.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(28209..29072)
                     /locus_tag="ACHGMT_000544"
     CDS             complement(28209..29072)
                     /locus_tag="ACHGMT_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFN6834316.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(29268..31367)
                     /locus_tag="ACHGMT_000545"
     CDS             complement(29268..31367)
                     /locus_tag="ACHGMT_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFN6834317.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(31587..32696)
                     /gene="rpoD"
                     /locus_tag="ACHGMT_000546"
     CDS             complement(31587..32696)
                     /gene="rpoD"
                     /locus_tag="ACHGMT_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFN6834318.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(32720..34561)
                     /gene="dnaG"
                     /locus_tag="ACHGMT_000547"
     CDS             complement(32720..34561)
                     /gene="dnaG"
                     /locus_tag="ACHGMT_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFN6834319.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(34743..37058)
                     /locus_tag="ACHGMT_000548"
     CDS             complement(34743..37058)
                     /locus_tag="ACHGMT_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFN6834320.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSFAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(37243..38103)
                     /locus_tag="ACHGMT_000549"
     CDS             complement(37243..38103)
                     /locus_tag="ACHGMT_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFN6834321.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTVIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(38162..38794)
                     /locus_tag="ACHGMT_000550"
                     /pseudo
     CDS             complement(38162..38794)
                     /locus_tag="ACHGMT_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            38961..39722
                     /locus_tag="ACHGMT_000551"
     CDS             38961..39722
                     /locus_tag="ACHGMT_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN6834322.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            39748..42339
                     /gene="mprF"
                     /locus_tag="ACHGMT_000552"
     CDS             39748..42339
                     /gene="mprF"
                     /locus_tag="ACHGMT_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFN6834323.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(42527..43714)
                     /gene="tuf"
                     /locus_tag="ACHGMT_000553"
     CDS             complement(42527..43714)
                     /gene="tuf"
                     /locus_tag="ACHGMT_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFN6834324.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            44082..44438
                     /locus_tag="ACHGMT_000554"
     CDS             44082..44438
                     /locus_tag="ACHGMT_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFN6834325.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT        12437 a         9416 c         7600 g        15051 t
ORIGIN      
        1 cttctaccca ttcactcaag tattcccacc gtgccatttt ttcttcaagt tgtgcttctg
       61 tttcagatac gtcgttttgc aattcttgta atcgagtgaa attatctcct tgatgattca
      121 tctcttcttg gagcaatgag attttttctt caagttctgc aatctcatct tcaatcgttt
      181 cccattcttt ttgttccatg taagataatt tcttcttttc agcaggttcc ttgctctgta
      241 tttttggttt cgctttttca gcaggctgct gggtctcttt ttgcttcagt aaataactgg
      301 acatgtttcc gtaataaagt tcttgttttc cgtttccttg gaatactaat aacttatctg
      361 ctaccttgtc caagaaataa cggtcatggg aaacagtgat cacagcacct ggaaaacttc
      421 taaaataatc ttccaaaatc gtcaatgttg ctatgtccaa atcattggtc ggttcatcca
      481 ataataaaac attaggttgc tgaatcagca gtttcaacaa aaataaccgg cgtttttctc
      541 cgcctgaaag tttgcctatc agtgtcccat gcataaatct tggaaataaa aatcgttcaa
      601 gtaattctgc tactccgatt tgactgccat ctgctgtttt tgcttcttca gcagcttctt
      661 gtaagtaagc aatcatccgt ttatctggtg ccatttcttc attttcttgt gtataatacg
      721 caagatgaac cgtttcaccg atagacattg taccgctatc caggggaatt ctgccagcta
      781 gaatattaag caatgtcgat tttcccgcac catttttacc agtaatacct agtctttccc
      841 gagattgaat caataaatct aaatgttcta gcagtgtttg gtcatttata cggtaactgc
      901 catctttgat ttccagtact tttttcccta aacgttgtgt cgctaggttc agctctaaat
      961 catcttcttg gtttacttgg tacaagttct cctttagatc ttcgaaacgg ttgatccgtg
     1021 cttgctgctt ggtgccacgt gcctgtacgc cagccctcat ccaagctaat tcctgtttat
     1081 aaagccgctt gcgcttttct tcttgttcta cttctacacg atcacgttct gctttttcta
     1141 agacgtaagc ttcataattt cctttatact cgtataattt tccaaatgaa agttcgaaga
     1201 ttcggtttgc tactctatct agaaaataac gatcgtgggt aaccatcaat agtgcgccac
     1261 ggtattgttt caaatagttt tccaaccact cgatcgcttc ataatctaaa tggtttgttg
     1321 gttcgtctag taatagtaaa tcgggttcat caatcaacac ttgcgccaga ctaactcgtt
     1381 ttttctgacc acctgataaa gtactgattt ttttatctaa ttcactgatt cctaattttt
     1441 gcaaaataat ctttgcgttg gtatctgtag tccaagcatc ttctttattc atttgttctt
     1501 ctgctttagc ataacgtttt tgcacatcag ggtcttctcc gttttcccct agttcgatca
     1561 atgcttcctc atatgctcga acagtttgga tcaaggggct gtttccttga aagactgctt
     1621 ggagaacagt taattcatcg gaaaattcag ctgcttgaga aagataacca atacggtaat
     1681 cagttggata aaacactgtt tgccgatcac cgtcgctgct atcgataccg gcaataatat
     1741 ttaagagact tgttttaccc gtcccgttcg tcccaatcaa cccgatacgg tctttttcgt
     1801 ggaccaaaaa agaaatctgg tcaaacaaat ctttttcacc ataagttttt ttgaggtcag
     1861 ttacttttaa ttctttcaac atgttccctc cttatcttgg atattgtatc ataaaaatag
     1921 tgaacttcag gaaaattttt ttggtatttg ttctttttgt gttctcaaaa aaagaacagg
     1981 accgattcga tcctgttctg atatgttttg ttttttataa aacttgaaaa tttttacctt
     2041 tttattttat tggactcgtt ttttttgact ttgctcgaaa cgcaaatcgg aaagaaaagt
     2101 tatcagatgg tttgttcatc gtataagatt ctaacaaatt gatattcctg cgatagctgt
     2161 tcgcagaaaa cgtcgtgatt ttttcagcaa cttcgtattc atcaatgatt tttctttcaa
     2221 gataataacc tcttagcatt tttttcgtaa acagactatc ttgctggatc atccacgaag
     2281 aaatcaaatt ccccgagttc atttttattc cttcaagttt gcgttgctgg ataaaaaatt
     2341 gaaccatcgt ttcatcataa atatcagtca agttatctaa actttcaata atcaactgag
     2401 tatttttttg atatgtctct ctgatttctt ggatatcttc atcactaata aatgtctgac
     2461 gatcagcata tcttctttgc caaaacattt tcggatggag aacgattcgc attaacctac
     2521 ggacaacaat gaaccaaaag ccgataaaag acaaaatctg gcttgtgata gaatgttcga
     2581 atcgagaaag aaaacgggag taaaaacgat aaccattaga agaaagcttg cccgaacgat
     2641 aactttcatc caatccatcc cgttcgatcg acaaaatcag tgcttgtatt tcttgtactt
     2701 cttgcttttc atcatcggtt aaatcttcca agtacagatc acgtatccgt tcttgataat
     2761 tttctatcac tgcttcagtg gctaaaaatt cttttgtatt acgatcaatt tcctttattt
     2821 cttttcgcaa aacttcaacg acctcttcta aaatcatcaa agcattcaaa tccgtgcttt
     2881 tcgcttcctc tgtttccgtc agcataggca agacaatgat ccctatgacc aaagtcacta
     2941 agatgacacc cgccgtcaga aacaaaagca aagaccgctg gtaaaacatc atattattga
     3001 ttgaaaatgg caaaataaaa atcgtagcca aactgactgt tccctttaca ccgccaaatg
     3061 ttaagatcaa gatctcgttc aactgttttt tgaacttttt actgccatct ttgaatacat
     3121 aaaaaagact gatagaaatg aatctgatca caaaaagcat gacagagatt aatacgataa
     3181 tcgctaataa aagaccgttt ggatataaac cgtctcccca tactggcgag aacacttgcg
     3241 tcaattcgat tcctaaaaat ataaatacta atgcattcag tgtaaaagca atagttgtcc
     3301 atgtactgtg ggacaagctc gacaattctg catcaaacac agagattttc ctaaaaccag
     3361 aagcttggag aatacctgct gccactgcag caatgattcc agatacatca aatgcctctg
     3421 ccaatacata ggctagaaaa ggaagcaaaa gttcgatcag cagataccct gtgacatctt
     3481 gtgctgatgc tttttcgatc aaatgaatta ttttttgttt gaaccagaca agcaaaaagc
     3541 cgatcaaagc tcccccaata ctagaaacta ctagactcat ccccgcgttt actgctgaaa
     3601 aagatccggt gatcagcgca ccaagagcaa actggaaggc agtaacacca gatgcatcat
     3661 tcaaaagacc ttctccttcc aaaatatgca tcgccttttt agggatattc actcgtccag
     3721 aaagagaact gactgctacc gcatctgtcg gacctaacgc tgctccaaaa gcaaagcagg
     3781 cagcaagtgg gacacttggc aacaggaatg ataacgtcgc acctaccccg acaagggtca
     3841 aaatgactcc tccaaatgcc aagaacaaaa ttgtcccgaa atttttgaga atcgaagaaa
     3901 tatctgcttt ttctccttct ctaaataaca atggtgcaat aatcataact aagaacaatt
     3961 caggttcaaa atcgattgat tgtccccact ctgtcagacc taataccact ccaaggaaaa
     4021 tttggatcag tggagctgga atcattggca aaatgcggct agctatgttc gataaggtga
     4081 tcgttactgc aaatacaata actaactcag caaattccat cgtccatccc ctttattttt
     4141 tattttgtgt aagcattttt aaaaattgtt cttttttctt taaaattttg gtttcgacta
     4201 cttcaacctg tgtttggttc ccctctaatt cacactgtac acgttttgac tccaaacgaa
     4261 gcagatcatg tttcagacgt tccaactgac gatacatcaa tatatttctt ttacgcaact
     4321 tacaaagtat ttgacggcgg tcatccatcg aaagggcgcc atagcgatca tgcaacagtt
     4381 ttttttgttg ttcaaaactc tctctcattt tccagatctc ctatacacaa acttccatca
     4441 ttatagcaaa gttctttttg gatgaaaaat atttcattgt ttcatcgttt attgctagtt
     4501 agaaatttcc ataaattttt caacgcctca ttctattcca aatagtatca gatatcaagt
     4561 aaaacttaag aaagataaaa gtttctgaca aaaggatgtt tactttccca ttcctgcctt
     4621 tcttgttctt ttatcacaac aaaaaaatgg ggctgcgaca aaaatcttgt cacagtccct
     4681 tattctgaat aaacggtgtg gaagagacag cctatcagta gtaataagac acatctcttt
     4741 cacagcctca tttttgcgta aaaatcgatt tattcgaaaa attccccgta tacatcttct
     4801 acacgttgat ctggattgat cacgatatac aagttacctg ttttttcgct gatttttgtt
     4861 gattgatatt gattgtctaa tccttctgtt cctttatcta gatatgggaa gtacacaaga
     4921 tcaggtgtgc catttcggta aaggatttcc attcgctcga tatcttcgta tttcaatgcg
     4981 cgattaaaca tgcccatttc cagtccgcct aggttgatat catgtgtcgc aactgcgtcc
     5041 ccttcatgga agatttcgat tttaaatcca gcacacggat ggatttcaac aaattcactg
     5101 ccattgattc gtccaaaact tgttgtgatt tgtttgatcc aaagatcgcc gatatgacgg
     5161 cggttgatcg tccaggtttc tccatttcta aaataaaatt ttagcgctgt catttcttta
     5221 ctcattaaaa agactccttt atgatgtaat ttattcttgt actacctcag tttagcacaa
     5281 atttatgtaa gtggtcacat cttatgtttt ttcttctaat gacgtatctg cccactttaa
     5341 gtagccaaca aagctgagca gatacagtct agtgtttttt aaatcaattt ttctttcgat
     5401 aaaaaatcca aaacctgttt tttatcattt tttattcttt gttctacgac ggctttttca
     5461 gctaattgca aagcttcact aacccactta ccaggttttt ttccagtttc ttctagcaag
     5521 attttaccat ctactgctaa ttcgcttaat gacttgatag gtaaacgatc ataggattct
     5581 cctacggcat ccatctgagg tgtttcgtca aaatagatca tcaattcttc tacttgcagt
     5641 gcatgttttt tgcctaagaa gtacaattcg aatggttcca aagctccagt ttctcgttgt
     5701 cgtagtcctt ggacaagtgc ttgaacgtct ttaatcattt ggttagaaca cttccacgat
     5761 ttcaaaaagg aacgaatctc attttctttt agatccagtt ggctgatcaa taatgcccat
     5821 gcttggctct cttctttcaa tgggacggat ggtaattcag caaaacgtaa aagtgcttcg
     5881 ccttcttctt tcaaaccggg acagcaaatg tagcactctg tttcaataaa tgcatggatt
     5941 cccgcggccc gttcatttcc caagagcatc ttgatgaatt ccacatttat acgttctaca
     6001 gatattttac ctaatagctc gtggttttct ttgatggcgt agaaggtttc ttcctctaat
     6061 ttgaacccta gttgactgac aaaacgcaac ccgcgcatca ttcgtaaggc atcttcatgg
     6121 aaccgctcat gaggattgcc tacagctcgc aagatctgtt cttctagatc ttgcagacca
     6181 tcgaacaaat cgatgatttc tccatcttct ttcaaagcaa acgcattgat cgtaaaatct
     6241 cttcttttca aatcttctgc caacgaacga acgaattcga catgatctgg tctacggtag
     6301 tcctgataag ttgattccgt ccgaaatgtg gtgatctcat attgttcatc attttctaat
     6361 accaacacag ttccatgttc gatcccaata tctatcgttt ttggaaaaag ctgtttgatt
     6421 tcttctggaa atgcgcttgt tgcgatatcc acatcatgga ttggctggtt cagcaagata
     6481 tcacgaacac ttccacctac aaaataagct tcatatcctg cattctctaa ttttttcaga
     6541 actggaattg ccttttggta ttccagaggt aattgtttta atttcatttt tccaaccttt
     6601 cctgcgtgtt tcttgtcctg agataaagac tcagttgcat ggtgactgtt tgacaagtcc
     6661 gtttcagagt cttcaaggcc aggaccactt catcttgttt actcgtcctt acgctcaaag
     6721 cgattatggt ctctttgatt gaatttttcc atgttttttt caaaaacttc agttaagtcg
     6781 atattcaatg aattagccat gatcatcgta acgaataaaa catcaccaag ttcttctgct
     6841 accgagttaa ctggttcaga agcctttttg cttttttctc cataagtgtg gttgacttct
     6901 cttgccaatt cacctacttc ttcagttaat cgagccatct gagctaatgg agagaaatac
     6961 cccaccttga attgctggat atattcatcc acttcctgct gcatactgta taaagaacgc
     7021 tttttatcca tctttttccc tccaaaacta tcttaccaaa actttaacgc aaatcctaga
     7081 taagagcaat tgagaaaatc gaatttccat gctacattta ggaaggaatt tctaaatgga
     7141 aattctgtga aaatacataa ttgaaagctg gaattcatat ggtcaaacga aaaacagtaa
     7201 aagaagttct ttttatcatt ataggaacaa gtatttatgc atttggtctt gtttacttga
     7261 acatcgctaa tcatttagca gaagggggag tctcagggat caccttgatc ttaagagctt
     7321 tatttggtat tgatccagct tatacgactt tattaatcaa catcccattg attttactag
     7381 gaggaaagat cctcggtaaa cgttccttgg cctatactgt tctaggtaca ctttctttgt
     7441 cattcttttt atggatctgg caaagggttc cactagaaat caatttacag catgatctac
     7501 tgatcgtttc tctacttgcg ggtttagttg caggtgtagg aagcgggatc gtctaccgca
     7561 tgggtggaac aacaggcggc agtgatatta tcgctcggat tttagaacaa aattacggca
     7621 tcactatggg gcgttcccta ttggcatttg atatcatcgt cttacttgct tcattaacct
     7681 atatcgactt gaaacgtatg atgtatacgt tgattgtttc gtatgttttc agtcgtgtga
     7741 ttgattcgat cttagatggc ggatattctg caaaaggtct acttgtcgtc tccaacaaaa
     7801 gcgaagagat tgcaccacta ctgatgaccg gtctgcaaag aggtgtgact ttcctttctg
     7861 gagaaggcgc attctctggt aattcaaaaa aaatcatcta tatggtcgtt agctctagtg
     7921 aattaactga agctaagagg atcgtccacg agattgacga aaaagctttc ctttcaatca
     7981 tcaatgtgca tgaagtggaa gggcaaggct ttacttattt gaagccacag acaagattac
     8041 tgaaaaaact ataagaaaaa agacaaagat aggctatgag gaaagattcc acatccagct
     8101 gacctttgtc ttttttagta ctatactatt tttaactaat tgttatgttc acttacccgg
     8161 ttacttttct tttgtgtctg caaaaggatc tttcggttaa cttcatcaga taatttcaaa
     8221 aaagcatccc tatctccatc ttctagtgct tgatcgattt gagcataaag catttttagt
     8281 tttgcctctt cttctttttc ttgaaaatat cgttcaatta attcagtatc ttcttcactg
     8341 actgaagaat cccaagaaga aaatggattg tcttctaaga tagatagata ttctcttgtc
     8401 tgccaagcat tttcgaatat acattccaca tataaaggct ttttccaatt caaacggatt
     8461 tcatgaaaaa tctggtctgt gtccgtaaat tcttttccct gcaaaaacaa tttcatgggt
     8521 tccccttcaa cagatagatc actgatctgc agtcctcgat ccgcaaaatt cgccccttca
     8581 acaaagtgga cattgcttaa aatcgcttca tgattggcaa gataattcaa gatccaaaaa
     8641 acttctcttc ggctaaaaga tacatgattg accatccacg ttaaaaaatt cttcttttcc
     8701 cgtacatcaa ttagcatttt tctcacctct ctgttaggtc atctaataat gacagtgcct
     8761 ccatatctcc tggttcaagt gttaaataat aagtcaataa ttcttgcgct cttttcagct
     8821 gcccttcttc tcttaagaaa aaggcatact ctttcaagaa atctggttca tgtgatagtt
     8881 catgataagc ctgttcatag tgtacagctg caacggtaaa gtcttctaat tcattataag
     8941 catgggctag gttccattct gcataaggat tagcagtttc ttccatctga ttgatcactt
     9001 gtatcacatc ttcatatctt tcatcatcca agtaaagatt acttaaagta agtaatgttt
     9061 catcttgttt gtctcctact tctaaagctt tcagtaaaag ttcttctgct cgctgtctat
     9121 catgcagacg gaaagcattt tccgaggcca ggtgataaag ttcgacttgg taaggatttt
     9181 ctttgattcc ttcttctaac actgtttgtg cttcttcaat catttcttct tcttgaagag
     9241 cttgtcctag atatagatat aacgattgat aatgcggatt aacttctcga agttcttcca
     9301 gatagttgat tgcctgctga ttttctttca attgaagatt cgtaaaagct agctggaaca
     9361 gtcgatcatc tgtcctttct tctttcagtg cttcttctaa aggtacgata gctgcttcga
     9421 attctccttg catacttaag gattgtccta aacgttcctg catcgagatt cctgaaattt
     9481 catttatccc gcttgccaac aatgtttcat aaaccactgc tgcctctttg aaacgatcaa
     9541 ctgaaaaata caattcagct agcgcaaact ggatcaatgg ttcatcagga agcaacccag
     9601 ctgcttcttt caattttgct tcactcactt cagggattcc gataacttgg tacaaatcag
     9661 ctatcgccaa taagctttgc ggatagtaat cactttgctt atccactttt tccaggtatt
     9721 caaaagctaa gtcgatctca ttatcttcga ttgcaatttc tgctaacggg atgttcagtc
     9781 cgtcattttc tgggtattgg ttgatcagtt gttcaaaaat ctgttttgct tcgatcaaaa
     9841 agccaatagc cagcaattct tcgcctagtt cagctaatat atctgctggg tcttctttta
     9901 atgctttttc taaaaagatt tgtgcttgtg acaagtcttc atctgataaa gcttgcaaca
     9961 ttttttcgct atttgtattc atttcaattc cctcttatct cactgacgat ttgttgaaat
    10021 accgtcattg ttctcaataa ttctttttcg tttggatgtt ggtatccgtc tattatacct
    10081 attttttcta aaacaagtat ttttttgctt ttttctgcca aaaaaagaac tttttccaaa
    10141 tactccatct ttgaaatctg ttccagccag tggattggat agccaagcaa gtccagccac
    10201 acaaaaaagt tttttagttg gaaatcaaaa ggatatgctt ggagattcca cacgaaatga
    10261 aacatcattc ctaaaaattt tttcatacta gcagaaagta gatggccatt ttcggtcaga
    10321 taaaaagctt gttcaaacag tttaccataa tttgacaggt tttctgcctc taattgataa
    10381 aaatagatca tttcttcgat catcccggca aacggccgag tcgttaattg ctttttattt
    10441 ggatagtttt tatacagatt ttgcaaaaaa cgatgatcac aaatcattcc gcaacagata
    10501 aaagtcaaaa agtcaatctg ctttccgtcc acctgccgct cgctgattgt ttgatcataa
    10561 acgatgcgtt ctggcagatt gatggtttgc agcactacac gatttggtag ctgcaaaatc
    10621 gtccgctgcg gtgttaatgc tcggcttaga gaacggatcg atacagggat catccatagt
    10681 gatgaatcaa gggttgtatg tttttgaaaa aaacctgcca gatccatgat cccttcattt
    10741 ccaaatccga tgataaacat ggattgattt tcgggatagc gtttgacgaa atcaagcagg
    10801 ttctttaatt ctgttaaatg attgcattga gtgttggcac aaatatacca gtcattgttt
    10861 gcttgatttg caaataacgg gttgatctta tctgcaaaca aatcataata gcgctgattc
    10921 gttaaaaata agattggctg atcaatggaa agttctctta gctgactgac tagcgtttct
    10981 ccgtaaaaca ctttcgtcaa cgcatcttct acttgataat caagttccat acgcatccct
    11041 cctcttctta tttttatttt accattagta agcaaattct agaaaagata agagcaaagc
    11101 tcacctcata acttgcaaat aaataaaaat taaataaaat acatgtgttt tctatgtatg
    11161 acatacggat tcggaatctc tgtctcttat ttattccttt tctgctagga aataaaaaga
    11221 aaagcacttg tagccctaga gccacaagcg ctttttcaac tgtcttattt aacagcgtct
    11281 tttaatgctt tacctggttt gaatgcaggt actttgctag ctggaatttc gatttcttca
    11341 ccagtttgtg ggttacgacc tttgcgagct gcgcgttcgc gaacttcaaa gttaccaaaa
    11401 ccgattaatt gaactttttc gccgttagct aaagcatctt gaatagttga aaatacagca
    11461 tctactgctg cagttgcatc ttttttagtt aaatcagtag ctgaagcaac tttttcgatt
    11521 aattctgctt tgtttgccat ggatagattc acctcctcca caagagatat gatagaaata
    11581 tcatttagtt aaaactttct gagtggtcca gaagtttaaa aagaaatatt gattaacttc
    11641 aatattctgt tatcacgata tcatatgaac cgcttaatag caaggttttt accaattttc
    11701 acatgctttt tttagtcata gaaacgtaac aaacgaaact gtctttttct tttgaaaaaa
    11761 aaacgtcgat atggtaaaat gcctattttc tcctacgagg gataattcgg atggacgtcc
    11821 cttcaaaagt aaatgctttt ctgatttgat tttctaagaa acgttcataa gaaaaatgca
    11881 tcaattcctc ttcattgaca aaaacgacaa atgtaggtgg tttgacagcg acttgtgtag
    11941 cataaaaaat cttcaggcgt tttcctttat ctgttggtgt tggattgatt gctacagcat
    12001 ccataatgat atcattcaac acagccgaag agatacgaag attctggttc atacttactt
    12061 gttcgatcat ttcaggcaga cgttccaacc gttgtttagt cactgcagaa acaaaaacga
    12121 ttggagcgta atcaagatac cggaattcat cacgaatctc ttgttcgaaa tcacgcattg
    12181 tattggtttc tttctttaca agatcccatt tattcacgac gataataatt ccacgaccag
    12241 cttcatgggc gtaacccgct actcgcttgt cttgttcccg aatcccttct tcagcattca
    12301 gtaccatcaa aacgatatca gaacggtcaa tggcacgcat tgcacgcatc acgctgtatt
    12361 tttcagtatt ttcgtatact tttccgcgtt tacgcatacc agctgtatct atcattaaga
    12421 atttctgacc actttcagaa acgaagtggg tgtcgattgc gtctcgggtc gttccttcaa
    12481 tatccgaaac aattacacgt tcttctccta aaatcgcatt gatcaaagag gatttaccaa
    12541 catttgggcg accaatcaaa ctaaacttaa tcgtgtcgtc gtcctcttct tcagtctctt
    12601 cagagaaata cttcactgct tcatctaaaa tatcaccaat ccctagtcca tggcttcctg
    12661 aaacaggata aggatcgcct agccctaagg aataaaattc atagatatcg tttctcattt
    12721 ctggattatc tactttattg actgctaaaa tcactggttt attacttctg tataaaattt
    12781 tcgcaaccat ctcatctgcg tctgtcacgc cttcgcgtcc gctgactaca caaatgatta
    12841 catctgcttc ttcaatcgcg atttctgctt gatgcttgat ctgatccata aaaggttcat
    12901 cgccaagatc aattccgcct gtgtcgatca cgctgaattc tcgacctaac cattcacctt
    12961 ttgcataaat acgatcacga gttactccag gagtatcttc gacaatcgag atacgctcac
    13021 cagcgatgcg gttaaaaatc gtagacttac cgacgttcgg gcggccaacg attgcaattg
    13081 ttggatttgc catattattt cctcctcaca agtttccaat ctcacttagt ttaacgaatg
    13141 cattgccaac tttcaagcat agattgtcaa ttaaacgaag tatttttatt ccttattgaa
    13201 atactaatcg cttttcaaaa gaaaaactga gacaaaagca gctgctttca tctcagtcac
    13261 agatacaaaa tcaattattt ttcagaatca tcggttgctt tttcttctac ttcttctttc
    13321 aacgcatcac ctaaaatgtc tcccatagta aatccagtat tttcttctgg taattcataa
    13381 gactcaacat tttttggttc ttcttctgaa gctggttttt cttctaaagc tttgatactc
    13441 aatgcaatac gatgttcttc tggatggatc tctaatactt tcactttcac ttcgtcacct
    13501 tcatgaagca cttcatgtgg tgtcgcaata tgtttatgtg aaatttgaga gatatggact
    13561 agcccttcta ctccagggaa cacttcaaca aaggcaccga agcttgtcaa acgtttaact
    13621 gttccgtcta atactgtgcc aactggagct tttgtatcga tttctgacca aggtccaggc
    13681 tgtgtgtctt tgattgacaa ggaaacacgt tcttgttcag gattgataga caacactttg
    13741 acatttacat catcattgac agccaataca tcacttggtt ttgctacatg actatgtgaa
    13801 atctctgaga catggactag accgtcaatt ccgcctaggt cgacaaatgc gccaaaatca
    13861 gtaagacgtg caacacgtcc actgagaaca tctccctcat ggatagttga aagaatttct
    13921 tttttctttt cttctctttc gttttccact accgctttat gcgataagat caaacgattt
    13981 tctgatggtt cgatttcgat gattttaaag ctcaatgttt tgcctttata gtctgaaaag
    14041 tctgcaacaa aatgatcttc caccattgat gcagggacaa aaccacgtac accgacgtca
    14101 acgactaaac cgcctttgac aacatttgta acaggtgctt cgatgacctt gccctcttgg
    14161 aaatctcttt cgatatcttc ccaaactttt ttagcatcta aacggcgttt tgaaagtaga
    14221 tagctgccgt tttctttatc tttgccgatc gtactgataa cgactaaatc taatacatca
    14281 ccgactttta ctacttcgct gatatcttct accggcaaag ttgataattc ttttgccgga
    14341 acgactcctt caactccagc cccttcgatt ccgacaatca cttgtttgtc ttcaatgact
    14401 aaaacttcgc ccttaacaac atcgccaact tttacttctt gtacgctttt cattgcgtct
    14461 tccattgact ggttgttctc ttgattttga tcaaattctg tcataacact tgtcctccta
    14521 accaacattc tgccgtaagt acggtactgt ttttagttta attgattatt caaaaaaaat
    14581 aaagcgattt cgttcgtttt ccttgaaaaa acaattgttt ttcatttttt ttcttaattt
    14641 aaaaaagaag ataacctttt ttcaaaacaa cagcttttat tgctgcaacg acttctggaa
    14701 tcgatttgcc agtcgtatcg atacgaacag catcctctgc ttgcttcagc ggagagacgt
    14761 cccttgttga atcataatgg tctctttcct gtatctcttt cgtcagtgtt tcaagatcag
    14821 tcggaattcc tttttcttgg ttctctttga aacgtctttc tgcacgctca cttacgcttg
    14881 ctactaaaaa gattttcact tctgcattcg gtaatacagc tgtcccgatg tctcttccgt
    14941 ccatgacaac gccaccgcgt tcccctattt ttcgttggac agcaactaat tcttctctga
    15001 cttttccatg tgcagagact tcagaaacag cttttgtgac atctggttga cggatttcta
    15061 aagtcacatc ttttgatgct atgaatacat gctgtccatc ttcttgttgc gaaaaagaga
    15121 taggatactt tttgatcagc gaaactagcc cttcttcatc tgaaaaagat acattgttcg
    15181 taatagctaa ataagttact gcgcgataca tggcaccggt atctgtataa atataattca
    15241 attcttttgc taatatcttg gcaacggtac ttttcccaga tgaagcaggc ccgtcgattg
    15301 caatactgat ttgtgtcatg atataactcc tttttctcag aaaaaaagcc gcaaagcgcg
    15361 gctattatta tttaattctt aattcttgac cagggtaaag cataaagttg tttggatcta
    15421 agccattcaa ttcaaacaat tgatcagtgg tgatccctgc acgctcagcc acttgttttg
    15481 gaccttcgcc tgcttgaaca gtcgttgttg aaccggctgc agctgatgaa gaaggtgttg
    15541 tttctgctgc attgctgctt gaatcccctg cttgataagt tgcctcactt gaggaagtcg
    15601 gtgttgtctc tgctgcaata ctgctttctg acgttacacc tgtatcttga gacacagtag
    15661 attcttccgc gattgtacta gattctactg ttgaagtctc agttgactcc tcttttgttg
    15721 tacttgtaga actttctact actgatgtcg aagtttcaga agaagttgct gctgttgcat
    15781 tgttgcttga tccatttctg atccagaaat aagcacctgc tggaatagcg atacataaga
    15841 ccagtaaaat caaaaatagc gaaagaaata atgtatttcc tttcttttgt tgtctctgag
    15901 aacttcttga tgaaacttca tcatctgtat cgtagattgg ttgttcccat ggttcttgtg
    15961 tttcgttatt ttctgtagat tgatgtctat cttttctgct cactgtttta cctcctaaac
    16021 tctatcgtgt gtattatacc ataggaaaaa gtgtcttgtc ctaaaaaatc aatgttccat
    16081 gtttttttta aataaattta gtaaaatcgt tccccaatga agttgttcag tcttagaaaa
    16141 aagcttcctc tttggcagaa gttctgcttt tgctccatca atatcacaac aattctgcgg
    16201 cttttcggag agtttttcgc caaaataagc taagatgaat tttctgcggc agttttcttc
    16261 attaatatag cgaagcatct gctgcaggcg gaattctttt tctttttcct gacgtttgag
    16321 ccgttccacc caattttctg gtttttcaga ctgctggatc agctccatcc attttttttg
    16381 cagctcatca aagggagctt gctctgtggc atattccaaa taaagctcaa aactttgtcg
    16441 ctgttctcta gaaagttgat taaagaaata atggatacgt tcatccccac tttgataaaa
    16501 aagaattgct gcactttctt cctgatctct gcctgctcga ccgatttctt gtacatagtt
    16561 ttctagactg tctggcaaat cataatggac cacataacgg atatcagatt tatcgatccc
    16621 cataccaaat gcattcgtag caatgagaaa ctgcagctga ttttttacaa actgctgttg
    16681 aagttgtctt ctttgagctg catcaagacc accgtgatag tatccaactg taaaacgttc
    16741 cctgaataaa tgatacaatc gttcgacttc tttttttgtt gcacaataaa tgattgccgc
    16801 tccttgtgct ctttccatga agtgttcaag ttcttgttct ttttgctgcg ttttccgaac
    16861 aaaaagcgag atattttgcc gattgactga ttgtctgatc tcttttggtg gtcgatcaaa
    16921 tagtacatga cgtatatctc ttgctactaa atctgttgct gtcgccgtta gtgccagtgt
    16981 gaccgggttt cctaaaagct ttcggatttt tcctaactgt tgatattctg gacggaaatc
    17041 aactccccat tgagacacgc aatgagcctc atctactaca tataacgcaa tattttgttt
    17101 tttcagatgc tctaaaagag aaggattagt caacatttcg ggactcaaaa acaaaaattt
    17161 atattgcgat agatgacgga ggacgtatct tctttcagat tgcgaaagta aactattgaa
    17221 agcagctact cgtttctccc ctcgtttttg taattgcgtc acttgatctt ccattagaga
    17281 aagcagcggt gacacgatga tgaccagacc ttccattaaa taccccgtca attgatagca
    17341 aaggcttttt cccgttcctg taggcaaaat agcgagagta tcttctccat caagcaacgc
    17401 ctgaataatt tcttcttggc cgggacgaaa tgaatcaaat ccaaaatatt tttttaattc
    17461 atttattaac attctgtatc gcttcctttc tcaatagata gatttgatac aaacgaaatt
    17521 ctccataatc taattgtcca tctacattcc attcgctaaa gcgccactct cgcacggaat
    17581 ccttatttgg ttccaaacgt tcgattgttt ctgcagagag aatccactcg tatggaaaat
    17641 catcaaaaaa caaggcccac tcaataagat gatctgttac tgttcctctt tttaaatgac
    17701 ggattgccat gatctggtca attgtctgac cattcataaa catctgcttg gtgatcatca
    17761 tgctttgatt gaaattttgc tgtaataatg aatcgatcaa agcgtgccaa agcgaatctg
    17821 gccgtttttc gatttgtgct aacaaaaggt ggatacatct gctttgttgc agataaaccg
    17881 ctgactcatc attgtttttt gccagctgat aaggaagcag ccctgttcgc tcatttccag
    17941 ataattggtt agctaaaaaa tctgctgctt cttctggtat ctccgaaaac agctgcacca
    18001 aactctcata cgcagtatca atcaaaattt cacgtggcaa ttggctttca ctaagccatt
    18061 tttttagttg gaaagtataa aaaggacttg tctctgctgg aagatagtct tgaatacccg
    18121 ttgccagatt agaaattact tgaacagcaa atttaatgag ccgccaacta gtttcccctg
    18181 ttctgccata gcggtcaaaa cgcaaaccag tatgttctat ttgatgttcg gacaacatat
    18241 cagcccctgc aggcgttagc tttgcttcat tttcatttat ttcgatccaa ccttgctgcg
    18301 caattttttg caaaatttga taaaacttat cctgcttcaa ttctggaaaa ctgttatgga
    18361 taaacaaaag ttcgtgggta aatccataaa taagtactga agtcgttcgt tttcccacaa
    18421 ggagttggta aagagaacta atccgcagct tgtgtcctga tgaaaatagc gataaaataa
    18481 aaacaatcat attattctcc ttaagttagg atggttagaa atgtcactac tatgtaaact
    18541 tgtacctgaa cgctgtattg catgcggtct ttgtcaaatc gaagcgccag atattttcga
    18601 ttatgatgaa gaaggaatcg ttttatttgc ccaagaacca gaggctcatc aacagttcgt
    18661 gatgccggaa gaagaaacat atgtaaaaaa agcgtatcaa aaatgtccag ttcgagcgat
    18721 tttattagaa aataaaatat aaatataaac gaaactttaa aaacgtacac taagttctct
    18781 aaacttgaaa ataagccggg atctgtttct cttgttccgt gaaatcccga cttattttga
    18841 aagattcatt tgctatctat agttaattga ctagtttttt cacggaccct ataggaccca
    18901 tttatattat ttcaactttt tcagccagtt ttccaatgta ttgacgatca cagtttgctg
    18961 gtctgcgtta gaaatttctg cctctccatc tcctttttgc tcaccatagc tgccaaaacc
    19021 tccatgattt ccgccattga ttgatacata ctctgttctt tccggtaaat actgcttagc
    19081 ttcttgataa gcttcctgat tcaatacttt atcttttgtc gccgtgatcg ataaaacagg
    19141 aacagctgat tctgtcaatg ttcctttttt gtcgggataa cttgccatga agaatacccc
    19201 ttttaaatgt ttgtctgaat gattcttatt gacataacga cttgccatta cgccacctaa
    19261 tgaatggcca ccgatcacat aatcggaatt tggaaaatct tctttgacct tatccgcttt
    19321 gtttccgccc atcactgcta gatcaagtgg cattttgaca atagcgactg aaaagccgtt
    19381 ttttgctaaa tcttgcgccc aaatactata actttctggc tctacgagag caccaggata
    19441 aaaaataagt aatggagact ttccatgatt ctttttctct ccttcaaaaa ataagtaatc
    19501 ttctttgttt tttccttctt ttaccaacac ttgcgcttct gacgatggtt cgtacgtatt
    19561 ttgctgtata tagtaataac cggctccacc gagtaatcct aaaatgatga ctaagccaat
    19621 tagtattcgt ctaaatattt tcactttctc ccaccttttc tatctgagtg accccttttt
    19681 cttaatcaag tatgattata ccttaaaaaa ataagagaga aatgcaaact ttccagcatc
    19741 aaataaaaaa agacggaata tccatttgaa tatccgccct ttttgtatgt ttatttttat
    19801 ttagcacata aacgatacac agcttctgca taaatttcca ttgctttata catactactt
    19861 aattcccatc gttcattcgc ctggtgcata aagtcaggag tatcagggaa catcgcaccg
    19921 aatgccacac actggttcat cgtacgagca aaagtagcgc cgcctgatac aaacggttca
    19981 gtcatgtctc cagtaatatc gcgatatgtc cccaatagat ttttcactaa ctcactgtct
    20041 agtggcacgt ataatgaatc aaggaagtca aattcttcat aagtcaaccc atatttttct
    20101 gctgttttag ttaatttagc tactaaatca tctttcttga atgtaacagg gattctcata
    20161 tctacaccga tttttgtttg ttctggtgtg atttccaaac ttgcaaagtt catcgttagc
    20221 tcgccggatt gttcatcttc tgttttgccc acgacagctt caccagttgc attttcttga
    20281 actaattttc ctaagaaatt gattgggcca aaatcaaata cttctgaaag agcaattgcc
    20341 agacgagaaa tagcattcac tccttgtgct gcatctttcg cgtggatact tttaccaaga
    20401 acgacgatgc cttcaccttg ttcttcgaaa tcgaattcgt gttttttcaa tgcttctttt
    20461 acttcagaaa gcttttctcc agagtaagga gctgcatcag gaacaacatt caacgctcct
    20521 ccagctttta cagaaaactc gttcgttcct ggtcctgtca agtaagcttg caataagcct
    20581 ttttctgcat aaattaaagg aaattcagca tctggcgcaa atccttgcgt aattccttct
    20641 tctttttcgt tatatttttc taaacaacgc cataaatttt cttcatctgt accaaagata
    20701 aaacgaatac gtgtcttgaa ttccacgcca gcatcaatca aagctttgac ggcatacatt
    20761 gccgcaatag aaggaccttt gtcatcttgg gatccgcgac caactaacca gccgtctttt
    20821 atagttggat cgaatggttt tgtttcccaa ttattttcgt cccctgctgg aacaacgtcc
    20881 atatgacaca agataccgaa taattcatct ccagagccaa tctctgaata accgtaatag
    20941 ccttctggat ctttaaatgt tctaaatcct agcttttcac tgatttccaa tactttatcc
    21001 aaagcttcaa tcactttttt accgaaggga tgtccttgtc ctgaatctgc ttcatctagt
    21061 acggaaggaa ttcggatcaa ttcgctcaat gattctaatg cttgttcatg atgttgttct
    21121 gtcacaaatt ttttcatgat attatcctcc taatatgtta aaaagggtca tgtcaaattc
    21181 ctccgacaca acctcttctt ctcaaataat gacgttttaa gcctgctcca tcagcaagca
    21241 gaatgttctt aacaaatgat gaaagaaaaa acactcactg aatggagcta ttgacttatc
    21301 cacgtgctta tcgatagccg tttatcggtt tcttcattct tatgatagac cttgtacctg
    21361 tatttgtcta ggtttagaac attgctgcta cacctaggat cacacagatg gctacaaata
    21421 atatcgcaat taatttaccg gtaaatttcc accacactga aacatcgaaa cgagcaattg
    21481 ctacagcacc cattacgata gcagatgttg gtgtaattaa gtttactaaa ccagatgcag
    21541 attgataagc agtgattact aaatccttac cgacgccggc aaattcaccc ataggtccca
    21601 tgattcccat tgttgcagca gctaagccgg aagttgaagg aatcaagaat gacataggga
    21661 tatagaaaat aaatgtaata atgatgaata cacttgaaga taatgaactt aatccttgtt
    21721 cgccccaatg taagatggtg tctgtgatta atccattgtt cataaccact tggataccac
    21781 gagcaacagc tactacgatt gctacaccga tcaaatcaga agcaccagct aaaaattcag
    21841 agacaaattc agactctttc atgccataag tgattgctac tacgatggac attactaaga
    21901 ataacatagt aatttctggg aagtaccagt cacctaatgg aagcatgctt ttacctagaa
    21961 ccgtacctag taccggaagg tttgttaacc agtttgtcaa tgattcaaaa atcgtgaaat
    22021 tcgcattcaa ggttgtccaa ggaatcaaac tgatgatcat gataccaaaa gtaataaaga
    22081 acaatacatt aacatgtttt tgttttttcg tgattacttc ttgacgaccg attgcttcaa
    22141 tatcgaaatg cttcatatct tctttacgtt ggttgtatac taatgatttt gttggatctt
    22201 tttcgatctt tgatgcataa cgatacacat atacaataga aatagctaac aaaataacga
    22261 acatcaagaa tcgaagacca ataccttcac ccatcgaaat cccaacagct tgtgaagcta
    22321 cgcctgttgc aaaaggattt accgttgaag ctagacagcc tacttgagac cctactaata
    22381 cgattgctac agctactact gtatcaaagc ctacagccat catgactggg atcagtaaag
    22441 catagaatgg aatcgtttct tctgccattc cgtaagtaga gccgcctaat gcaaataaca
    22501 gcatcaatac tggaataagc attttttctt ttcctttgaa tttcttcaca actgaagcaa
    22561 tcccggcatc tagtgcccct gttttgttga taatacctaa aaatccacca acaaccaaaa
    22621 tgaagaaaga gattgggatt gcagcaggtg tttctttagt tagctccccc gctggtacac
    22681 ctagcatccc attgattggc gccatgaaaa tatcccacag cccttgagga ttttgttgta
    22741 ctcgttcata tgatccagcg acgatgttgc ctgcatcgtc tacactatag tgcccagctg
    22801 gtataaacca agtaagtaca gcgattactg caataataat aaataagacc gtatacgctg
    22861 aaggcatttg aaattttttc ttttttgttt gttccatctt cttcttcctt tcttttccgt
    22921 tagagacaac aattttggct ctaatgttat tttatcaaaa agagtatcag aacaaaaata
    22981 ataacacgca ttttgtaaaa cttttgttca aaaagttcgt ttttccctat tccctcctct
    23041 tttttcaggt ttttaaagct ttcttctttt tcattgtagc gtttcctttt ttttataaac
    23101 aaacattttc gtcttttaaa aggagaagaa tttcacattt gcaaagaatt gattgatttt
    23161 tatgcataaa tcaaaaaaat actcaaaaca aacaaataaa acgtttacat tattgcatgt
    23221 cttgtatttt tttccaaaaa aaaagaccaa gaaatttctt gatctttttg atatttattt
    23281 gactgctgta tgacgttgtt cgaactgatg ttgttttcga cttaaccatg gaagaagttg
    23341 cgcatgcagc actaaaaaga cagcactcac gatgaatcct ttgatcaagt tgaaaggaac
    23401 gatcccaatc agtacatagt ttgctaaaga catgcccagc atctgtgttg cgttcagccc
    23461 taaaaacatt aagtaaagag gcgtaataac gaaataattt gcgatactca taaaaatagt
    23521 tagagcgatg gttcctgtaa tgacacctaa aaatttgttt cttcctttat gctgtttctt
    23581 tacactaaag aaataataaa tcgggaatgt aaagatcgtt gttgataaga aactagcagt
    23641 gtctcctacc aaattatccg gagaaaaacc tgtagtcagc agatgtaata ctgaacgcaa
    23701 gaacgctgtt gcaactccgg ccgctggacc gaataagaac atgctgatca ataccgagat
    23761 atcactaaaa tcgatcttca aaaaactaaa agccggcatg atcggaaaag caaaatactg
    23821 taaaactaat gcgatcgcag caaacatcgc tactgcaacc attttctgta cacgggtgtt
    23881 cttcatttga aatcctcctg ttaggactcc tcttcagcga actttttgac gtttctctcc
    23941 aaatgaaaaa actctatttt ccagggaaaa tagagttaga gttatttgat caagcgtcgt
    24001 caaataagct ctatcttctt tatccagact atactgtcgg tatcggaatt tcaccgattc
    24061 agctattttt ttgcaaaata ggtcgtggac tataaccacc ggtcgggaat ttcaccctgc
    24121 ccctgaagac gaaccttata taatttttgt ttacatattc attatacata tctattcaaa
    24181 aaacgcaaca tctaacactt ataaaaagta agtgttcatt ttgagtggtt cagcaaactg
    24241 ctgacttctt ttttgttcag ttcacggtaa tccccaggac gtaggccttg taaagtcaga
    24301 tctccgtatt tttcacgttt tagtttttgc acaggaagac caactgcttg aagcatattt
    24361 ttcacttgat gattacgtcc ttcgtggatc gtcaattcca cgatactgtg gtttgtctta
    24421 ggatccactg ataaaatttg gaatttagct ggagatgttt ttcgtccttc gatctttatt
    24481 ccttttgtca aaggtgcaag catcgatttt gtagcgatcc cttttacctt tgcaacgtaa
    24541 actttatcca cttcatgttt tggatgtgtc aggcgctgag caaaatctcc atcatttgtc
    24601 agtagcaaga tccccgaagt atcgtaatcc agtcgaccta caggatagat acgttctttt
    24661 accatcggca aaagatccgt aacaactttt cttcctttgt catctgcaac agcagaaata
    24721 actcctttag gtttgtacaa taaatagtaa ccgtattctt cttgataaat tgggacttca
    24781 tccacttcca cagtgtcatg tttcccaact tttatcccta attcccttac aacttgtccg
    24841 tttactttta cacgtcctgc aaggatgaat tcttctgctt ttcgacgaga agtgatgcca
    24901 gcgtgggcaa ttactttctg caatctctcc attacaatta ttctccttta ttcattccat
    24961 caaagaatag atctggtgac acgtcttctt caagtgaaat ttccatttct tgtgtatcag
    25021 gcagttcttc taatgatttc aaaccaaaat aatctaagaa gtagccagtt gttccatata
    25081 atatcggccg acctggtcct tctactctac ctttttcttc aatcagttta tgggcagcta
    25141 gacgctgtac cgctcctgaa acctgaactc ctcgaatctg ctcgatttca gctcttgtga
    25201 ttggctgttt ataagcgatg attgctaagg tttcgacggc ggcttgagat aatgtgtttg
    25261 atgtcccttg cgcatatcgc ttaagtaagg gagccagctc ttttttagtc gttaatacat
    25321 aatggctttc cgtttcaaaa atatgcaaag ctgagtgcga gttttgagtg taagattctt
    25381 ttaattcttt cactaaactt gtgacttctg cttcgctgag atttaacagt ccgcttaatt
    25441 cttcattact gatcccttcc tcgccaacaa taaaaagaag ggcttcaagt tcacttaatt
    25501 tcgtcatgtt cggtatctcc tactgctgga taaagcataa ttgattcata gttttctttt
    25561 tgttgcgcaa tgatctgccc ttttttcatc aattctagca aagccataaa agtagtgaca
    25621 acttcacttt tagaaggtga ctcaaaaaaa gaatcgaaag tacagccatg cgcatttctt
    25681 tttatacgct cttcgataaa taccattcgt tcttcgatcg ttgtgtcatc gggcgtgatc
    25741 gttttttcca ctttttgaga aaatttctta cgttctaaga ttttctgcat ggcacggatc
    25801 aaatcgattt ttttgacttg tcctcgcttc aacggctttt cagcatccgc taacccatcg
    25861 atgttcatag gttcttttgt gtaataaaga cttcgttcct ctgctttttc tgtcagtctt
    25921 tcagctgcat atttatattt acggtattct aaaagctgag tgaccaattc ttctcgagga
    25981 tcttcttccc ataattcttc atcgtctgtg acgaattctt ggacaggaag caatgtcttg
    26041 cttttgattg ccatcaatgt agcagccatc actaagtatt ctcctgcaac agctagctcg
    26101 agtgttttca ttgtatggat ataattcata tattgctccg tgatcgtagc gatcggtata
    26161 tcatatatat cgatttccat ttgctgaatc agatgaagca gcagatcgag aggaccctcg
    26221 aatacatcta atttaatatt gatctccgtc aactcattca ctctttcttc tttttctttg
    26281 ctagccattt tccaatgtaa ggctctgtga tttcagttgc acggatggag acatccgggt
    26341 acttttcctg taattcatct aacatttgaa agcccatatt ctcaccgcga taagacggat
    26401 tcaaagaaat gtcatggatg atcagctgat cgttcgttgc ccagtaaata ccaataatcc
    26461 cttgaatgtt ggttgaacct tctggcgtaa agaaaaaaag ttcataatcg tcttcagatt
    26521 cgtacgtatc aatctccttc aacaaagaag ttttacctgt cagtttctta tggaaactca
    26581 atagtcccat agcaatttta cggttttctt tccggtaatg tgttaacatc ttctcacctt
    26641 cctatgacta agcccttgga aaatgctgct tataaacatc cgtcattctt ttcttcgtga
    26701 tatgtgtata aatctgagtc gtagagatat ccgcgtgacc taacaattcc tgtactgtcc
    26761 gcaaatcagc tccattttct agcagatgtg tggcaaagct atgccgcagc gtatgtggtg
    26821 ttacttcttt gtaaatccca gcctctcgta ctaactgctt cagatttttc cagattcctt
    26881 gccgagaaag acctttccca tgattattga caaatacatg catttcagat gcgtccttta
    26941 caagtaatgg acgtgcttcg tctaagtagc gttccagcca ttggatcgca taatcaccta
    27001 aaggaatgat ccgctcttta tctccttttc caactgtctg taccagtcca agagaaagat
    27061 gaagatctcc caatttcaat ccaatcaatt cactgacgcg catcccagta gcatacatga
    27121 cttccaagat tgcacgatct cttatcccta acgtttttgt cgtatcaggt gcttcaatca
    27181 gcttttctac ttctgtcaga gtcaatgtac ttggtaattt ctgtgctttc ttcggcgtat
    27241 cgatatgttg catcggatca tgatcagtga tacgctcttg ccgtaaaaac tgatggaatc
    27301 ttctgagact tgagatcatc cgagtaacag ttgctgtcgc ttttttttct tcatgcaact
    27361 tctccagaaa acggatgact aaaaagcgat cgacatcctg ccatgaagca attttctgct
    27421 cctgtagaaa atgcaagtac tgttccaaat cacgttcata gctttgtcgt gtattggcgg
    27481 acaatccccg ttcaatattc aagtagtgga gataatcgtt gatttgctct ttcataataa
    27541 tccccctcat gcctctctat catatcagat accgcttcat tctgatagtc tttttccata
    27601 atctgagcaa agaaaaagcc gtctagacac tcagacggac tggtttcttt tttctttttc
    27661 atagcattcc tgacagattc catggaacgt tagccgatga tcttttacta aaaaatgata
    27721 acgttcttct actacttgct ccacttcaag taaaagatcc tcttctactt cctcgatcgt
    27781 gccgcagttc aagcatagta aatgatgatg gaaatgtttg gcgccttctt ttctcaaatc
    27841 ataacgggct aatccatcat taaaacttac tttatcgaca attttcaatt ctgtcaagat
    27901 ttcaagggtt cgataaacag tagcaagtcc aatttccgga ctcttttttt tcactaaaaa
    27961 gaagatttct tctgcagata aatgctcttt ctcattttcc aaaagcacaa gcagagtagc
    28021 ttctctttgc ggtgtcagct taaaccccgc ttcatgtagc tgctgttttg tttttttcat
    28081 cgccgaaata gcattcatga ccatttactc aaccccaaca atttataata attacaaata
    28141 aaaaaagata taacactatg ttaattaaaa ttattataag ctagttaaga aaaacatgca
    28201 agggattctc aattagattt tttgactagt tttgtcacac cgttttcttg tgtaatcaat
    28261 ccttgtttca aaagattacc taatgcgcgc ttaaatgctc ctttgctgat accaaaagct
    28321 ttcatgattt cttctggatc cgatttatct gtaaagttga tttgctgatc tgcagaacgt
    28381 tctaaaatgg ttaaaatcat ttgtgcatca tctgatatag cttcatatgc tcttggcttc
    28441 aatgaaagat tcagtacacc gtctggtcgg acaccaatca ctcgaccttt tatctgctcg
    28501 cctagacgag gttcattata tcgttcagaa ggatggataa acccaagata gaaatcttcc
    28561 gttaaaacgt acgtacctgt taatttcaag cggtatgctg ttcctatcac atctttgttt
    28621 ttcatttctt ctgttccata tttgcttaaa gatttgaata ttttttcatc tgctaaactg
    28681 ccccaaatac ggtcttttgc atcaactcta agggaaatca tcaagcgatc ccctttttta
    28741 ggccatagct ctgtcattgt cggcaattca tcaagagata cgacaaagtc tttatctgga
    28801 agaccgatat cgacaaacgc acctaaatct tttcgtgtag cagtaacgat tccaaaagcg
    28861 taatgaccga tacggctttt aggaatttgt gtagtgatcc gattttcatg tttttgattt
    28921 tggtaaccga atccttcaac tgcttcaccg attttgtgtt caccttctga tttgcttaag
    28981 ccaaaggttt gtccgttttt ttggacaaag tagttttttt cattttcatc aataataata
    29041 cctgtaaata tagttgctaa taaatcgttc atttttttct ccctctatag tgaaaggttt
    29101 tcttaataca tttctttgct atcataacat aatgttatct acttgaatag ctgagccttg
    29161 gtttggaaag gtgaaaaaag gagtatgaca acagttgtgt catactcccc tttttcgaat
    29221 aaacggtgtt aaacggcagg ctcacagcct ctttcatttg cctaaaatta attgctccca
    29281 tttcctccga accatccagt tttacagttg atggttacga tgtaaaatgg caaagcactt
    29341 tctccaatgc ctttcatcaa tgcagatttt gttgaagtgg catatagatt ataatagcct
    29401 tccccatttt cgattctggc tttttctaat ctgtaacctc cttcaacgat atatccacgt
    29461 tggatacatg tagcaatcac tttgctttgg acacctggtg cccacgtcca tgctggatta
    29521 gatgcatcgg caatatcact tgctctggca gatttgattg gctgattcgc tgagtttgaa
    29581 gaggaagggg tcgaggatgc cgcttcttgc tgtgctgctt gtgcgaccgc agcttgttga
    29641 gcggcttgag ctgcagctgc tgcttgagcg gcttgttttg cttgttcttc agctgctttt
    29701 ttcgcttctt ctgctttttt cttttcctct tcttcttttt tctctttatc agtgagcatc
    29761 ttatctactt gtttcaattg ttcattcaac tgagcagata gatctgtatt tttcactttt
    29821 tctactgctt cttttgccgt cttgtattgg tcgcgtgttg ctgaatccac tacttttccg
    29881 tctttaaaga ctatagctgt tttttcttta gctgtttgca attcagcata ttgattttta
    29941 gcttctgttt gtgcttgttg aatcaatttt ccaaaatctg tgttatccgt ttcgattgct
    30001 tggatctctt tatctgcttt caacttgatg tcatctgcta gatgatcatc gacgatggca
    30061 ggtgctatga accactcatt cactttttgg atatctcgga tcttagattg gatatcttgg
    30121 taagttgttt ctaactctga atatcctttt tcattcttaa gactggataa atcatttttt
    30181 agttttgata aatcttgatg aatcatatct gcacgaataa attgatggtg atcatctgta
    30241 taaaaggcag caagtttctc atgaagttct tccatttgtt gctgtctttt tgcttctgct
    30301 gctacttcgg cttctacttt ttgatgatgg tcataataag cccagcttcc tccaccgagt
    30361 agaagtactg ctgctaaaac gagataaggt actttttttg attttttctt ttgcgcttta
    30421 tctggatctg ttttttctaa gtttgattct tgatgtgttt tttcagtatc tggcacgatt
    30481 gttattgatt tcgcttctgc ttcaaacagc ttaggctggt tctcttcttt ttctattttt
    30541 gcttcaagat cagtaccctc tttgcttaca gattgatttt cagcatcgga ttcactattt
    30601 atttcatcgg gctctgttga ctttttgtct gtgttttcga gtatttcttt tttctctgct
    30661 tccggcagtt tattcgtttt ttccttttct aattgttttt cagtaaatac ttcagttttt
    30721 tctgtgatat ccgtttttac gtcttttatt tttactgttt ctttacctgt ggcgttttct
    30781 ctatcagagt ctttttcagt ttcttgttcc ttttctggct ctactttgtt ttcctgtgca
    30841 attttttcac tgatcatttt gagcagctct ttcgcgtgct cttcttttgt atcttcacga
    30901 tgtgctttga tataagcgga tagtatcgaa ttttcttcct cattatcgac gatttctttc
    30961 agttcagcta ctcttctttt ttgttttgat tcaagcgttt cttcttgatt ctttcctgat
    31021 tcttccgaag tctcatgaac agttgctttc ttctgttcat ttttctctat attagttact
    31081 tgatcgtgag caggtggttc ttcgtcttct ggttctgctt tttcgaataa ttccattaca
    31141 gattctagag gtaaatcttt caattccgac cattcgattg gatcgtttaa ttctgtatca
    31201 ggaaagactg catcttcagt tgatttatcc tgcatagctg catctgtatt tgtttcttca
    31261 attgattctt ttatttgctg atcttctgca gttatttgat gaccgcagtc tggacagata
    31321 gtattctgct ccattttttt cgatccgcaa tttggacatt ttttcatcac ttagcgatcg
    31381 gctcctttac taggtttacg ctaccattta atctatcata ttctatacgt cacgccaata
    31441 gcttattttt aggatttact taaaaataat gaatgaattt ccttcctata gaaaaaaaag
    31501 cttgagactg cgtagtctca agcttttttt cttttaaaat aaaccaaaca gttttactga
    31561 tttctcttct ggtcaatagt ccacgcttat tctaagaaat cttttaattg ttttgaacga
    31621 gaaggatggc gcaattttct taatgcttta gcctcgatct gacgaatacg ttcccgagta
    31681 acaccaaata ctttccctac ttcttccaat gttctcgtcc ggccatcatc taaaccaaaa
    31741 cgtaagcgaa ggacattctc ttcccgatca gtcaatgtat ctagtacatc ttccaattgt
    31801 tcttttagta actcataagc tgcgtgttct gctggacttg ttgcttcctg gtcttctatg
    31861 aaatcaccta agtgagagtc atcttcttca ccgattggcg tttcaaggga aacaggttct
    31921 tgcgcgattt tcaagatttc tcggactttt tctgttggaa gatccatttc cgcaccgatt
    31981 tcttctggcg taggttctct tccaaggtct tgcagcaatt gacgttggat acgtatcaat
    32041 ttattaattg tttcaaccat atgaactgga atacggatcg tacgtgcttg gtcagcgatt
    32101 gctcgagtga tggcttgacg aatccaccat gtagcatagg ttgaaaactt aaatcctttg
    32161 cggtagtcaa atttttcaac tgccttcatc agtcccatgt ttccttcttg gatcaagtca
    32221 aggaactgca tgcctcgtcc tacataacgt ttagcgatac ttactactaa acgaaggttt
    32281 gcttctgcca gacgctgttt cgcttcttgg tcgccttctt cgattttcaa tgctaacgct
    32341 acttcttctt ccgcagttaa aagggagaca cggccaattt ctttcagata catacgaacc
    32401 gggtcattga tttttacacc ggttggcgca gaaagatctt ctgtttttgc ttgttcagct
    32461 tttttctcag cactcttcaa gctgtgaatt gatggatcac cattttcatc caccacactg
    32521 atacctgcgt cttcaatctt ttggatcaat ttgtccattt catctgcatt caaagtgaat
    32581 ggtgtagcaa gctgattgga taaatcatca tatacaactt gtcgtttggg tttgttttct
    32641 ttgatgaaag ctgctactgc tgcttcatac gtttttttat ctgttccatt tgccataaaa
    32701 gaaggccccc cttgagtagc tatttggctt gttttaactg tttcgttaaa ttgatgattt
    32761 caatcgtcaa ttcaagctca agctgtttgt ttccagttcg tctggcttct tgctgcattt
    32821 gtttttttaa tgttatttca tcagctaagc cagatttgct gatcacatct agcaagtctt
    32881 ggatttctcg tggcgaactt tcttctgcca ccggtaaata ttcgatttcc accaccaacc
    32941 gccttaaatt ttcatcttgc aaaaagtcta aaaactctgc aaggttgaat tcataatgtt
    33001 gtgagacata tgcatcgaat aaaaaataaa cttctgcata tgcatcatga ataaactgaa
    33061 attctcgttc ttttaaaata ttacggacag aaacttcttt aaatgcacga tataatagaa
    33121 tctgctctgc cttttctact tgcgtcaaag gtctcttttt ccgtgcagtt tcttgtctcg
    33181 taacctgagt gtgttgactt tcctgaggtt cttgacgatt ttgctgattt gtccttcgaa
    33241 gatcgcgcag ttgtttttgt atcgtctctc tgccgatctg aaactctgtt gacagttgag
    33301 atagatacat atcttgttcg attggtgaaa caactcggct aagttcaact agtaattctt
    33361 cgaggtattc gatcttgtct ttttcattat ctaaattttt cccttgtttt aaataacgtg
    33421 atttaaaagt aaagactgtt tcgcgcccat gagccactaa ttcttggaaa gatgcttctc
    33481 catacttacg gagatattca tcaggatcaa gcttttctgg aacgactacg acagaaagtg
    33541 ctaatcgact gttttgccga agcaattcaa tccctcgatt ggttgcttca aaacctgcgt
    33601 tgtctccgtc atagcaaaag accaattctt tcgtcagctt ttccatttgt tggatctgtt
    33661 gagctgttag acttgtcccc atagaagcta atccagtttt gataccagcc tgccatgcag
    33721 caatcacatc cataaaccct tcgaacaaca aaatttctcc gtttttccgg atatcgcttc
    33781 ttgctttatc aagattaaat aaaacttgtc ttttattgaa cagttctgtt tctggactat
    33841 tcaagtattt gggctgatct ttttcctgcc ctttttcagg tgcaagccag cgtcctgaaa
    33901 aaccgatcgt ctggccttga gaattacgaa gtggaaacat cactctttga taaaaacgat
    33961 cagacatacg gccatcatca tgcgtgacaa acaatccaga gcgttctaac aattcatttg
    34021 aaattgattc attcttgaat acttgctgaa gaaaagaacg ctcattaggt gcaaaaccta
    34081 tgttgaattc ttcaattaat tctaaagaca acccacgact ttgcaaatat tctaaagctg
    34141 gctgtccagc ttttgtatgc aatagcatgt gatgatagat ttccgctgct ttttcatgag
    34201 cagcaatcag tttccccgta gcagattcag gattttggac agctatagta cgctgacgcc
    34261 attgatcttc taacggtatc tgttcgaagt ctgccacttt taccactgct tctggaaaac
    34321 ttagaccttc tagttcttga agaaaagtaa acacgtttcc gcctttacca caaccaaagc
    34381 aatgaaaaat ctgcttgtct tctgcaacag aaaaagacgg tgtacgttct tcatgaaaag
    34441 gacacagccc caagtaattt ttaccagact ttttcaactg gacatattgt ccaatcacct
    34501 caacgatatt cgtttgactg cgtactgttt cgataacctc ttgaggaatc cgttgagcca
    34561 ttttttcacc ccctgctcta ttgatatctt ttgggtttaa cagaagatat ttcaggagaa
    34621 taaccggtaa aaaatcgcac taactgaaat cagtgcgatt ttaaaggaca caattataca
    34681 tgtttgattg tatcatattt cattgctttt tcaagtataa cacatttcta tctcattaag
    34741 catcaatttg ctactctttt cagtaactga ttttgaatat cagccttcca gtacaatact
    34801 cgaagaacaa tatttacgag catgaataca aagaagtatc ctacgctctc tcccaaccct
    34861 aagtttactt ccaaaattcc atggaacagc attgcagcta catatatccc tacgatcgtc
    34921 gttactgcta ggaagatgcg caatgggttg aagggataac aagcttttac aactgccatg
    34981 caactgatcc caatcagcaa gtaatacatc aaagtagtag tttcagcatt tccccatgcg
    35041 aatgtttttc caagtagata caccgcaatg atattcacta ctacaagcaa ggcatttggt
    35101 aaagcattaa taagtgctgt aggcaaataa cggtaagtga tttttctttt atcttgttca
    35161 aaagataaaa agaaggaagg atacccttca atcgctaaat caatcagcgt gatttggata
    35221 gggataaacg gaaatccaat tgctgtaacg gcacatataa tcgataaaat aaatgaataa
    35281 atcgttttga taaaaaagat gcctgagact ttcgtcacat tgttgaccac tcggcgtcct
    35341 tcaaacagga cttctggcaa tgtagtaaaa tcagagtcca acaataccag attcgcaatc
    35401 tgacgtgttg ctccatcacc ttctgccata gcaaagctgc aatctgcttc tcttaacgcc
    35461 aaaacatcat tcacaccgtc gcctgtcatt gcaaccgttc tgccgctttc tttcaattca
    35521 tttaccagca atttcttttg ctgcggactc actcgtccaa aaaccgtata gcgatgagca
    35581 gcttctcgca cttccatttc ttcagtaatt tgagaaagat cgacatacgc atcataattc
    35641 ggtaatcctg cacgtctagc gatattggag acagtaaaag gattgtctcc agaaatcact
    35701 ttcagatcca caccttcctc atgaagataa gctagtgttt cttgagcgtt ttttcggatt
    35761 ggatcatcga tttcaaacag agcaaccggc tcaacaaatg ctggtttttc ttcgttcaat
    35821 tgctgatttg gagcgatccc caacataagt acgcgatagc cattcgcttg ggctgttttt
    35881 agactgtccg gaagccgatc ttcttccacc aaacgttcag gcgctcctac gtacacgact
    35941 cccagttctg gaaattccat cgcgccccat ttgcgatcag aagaaaatgc gactttgttt
    36001 gtcacatcat accgattaga aagctcaaaa taatctctca tagcccgcat agtgatgttg
    36061 ttatcactgc tttctgccag atacgtcccc ataatttctg ggatggcttt ctcgtacagc
    36121 ggatgtagtg cttctacccg ctggatactc attttccctt cagtaatggt ccctgtttta
    36181 tccaagcata atgtatcgac atgggccagc gtttcaattg aatacatgtc ttgaacaagg
    36241 attcgttttt ttgccagttt ggttacccct gtcgttaatg caatacttat tagcaatacc
    36301 aaaccctttg gaagcatgcc taacaaagct gctgcagatg caataacaga aattttgata
    36361 gaagcatcgc ggatgaacag tgcttccaaa aatagtatga tccccaaagg aataatcacg
    36421 aaactagtga atttggatac tttgcgaatc gaatctacaa gttcagaacg aatagctttg
    36481 tgagtctttg cctcgctagt caagcggaca gcataattat ctgcacctac tcggataact
    36541 tcagccatga cagaaccgct tgtcaaaaag cttccagaaa gcaattcagc tccagtttct
    36601 ttgacaatca aatcagattc gcctgtcaaa agagcttcat ttgcttctgc ccgtccagat
    36661 ataacctgta aatcggctgg tatctgttct cctgctccga tttttaccaa atctcctaat
    36721 accagctctt tagtagaaat cgattgttct ttaccgtccc ggatcactag tacttctgat
    36781 tgggaaacaa tcgataattt ccgtacaaga tttcttgcat gtatttcttg ataaattcca
    36841 ataacgatat tgacgacaat aattgctaaa tacgccatgt tggaatacgc acctacaaat
    36901 aacagacaaa tcccaatacc aaagttcaga aaattgaata acgtcaatat attatcgctg
    36961 aatatttgtt ttgtactttt tgatgcatct tcctggtagt cattttgctg gccagaagcc
    37021 attttctgaa ctacctcttg ctgtgtaagt ccattcattt tctttttctc cattttccgc
    37081 caactttcaa aaataatcac gtgtttaagt ctagcgtgtt ccaagattaa aaacaacttg
    37141 tttttataaa atttatagaa aaacacacga atgattttac aaaaaagatg ccaacctttt
    37201 tgtttgaatc acccgtgtgc tgtttttatt atcaatctat ctttacttat attgttcctc
    37261 caaacggctc atccaccaac ctagtgctgt accggctata aaaagaacag gacaagctag
    37321 taccccagca actcccaatc ccgcgtaatc ggtaggaatg atcataatcg ttgaagcaaa
    37381 aactacacct aaaatgaaat ggaatagctt gctatatgcc tttgagaaaa tgtaatccat
    37441 gatttttgac aatccaagaa cacaaagaat cccaccaatt ccgatcggca caatcacttc
    37501 aagtcgtact tctttaaatc cgtcagccat cgctttatac aatcccatat acaccaaaaa
    37561 attagaaggg ctcaatcctg ggacgatcat acccaaacca atcaatccgc cggcaatcag
    37621 ccatgttcca aaattctgat cgacttgccc gtcaaataat tgagcgccgt ataataaaaa
    37681 caaataagcc agaacaaaag taacaaccat gatgatcaca tcagaagtat ctcttccttt
    37741 tttccctgct tctttccata acgcaggaac agttccagcg atacatccga caaaaaacca
    37801 taatatcgtt gtttcatacg cacctaataa aaaactgact aaaaaagaaa gtagaaagat
    37861 gactgtcact ccgcctagac caacaggcag aaaaaacaaa acattttctt tgaaattttt
    37921 tgtaaggtgg gctaaaaaag aaatcagccg ctcatacatg ccaaagacag ctgctaatgc
    37981 accaccgctg actcctggca agataaatcc tgaaccgata aacattccct tcacaaaccg
    38041 taacatccag tctttggttg tgtaccccac ttcttgattt tccatttttt gccttttttc
    38101 cactatttgc gtactccttc tttcatttaa ttttattcat gattgaacat ttcctgctat
    38161 tttacttaaa gactttttca taggtctcta ttattttttc attcattttg acaatatcag
    38221 gataaaacat gttacctcca ttatgataga gatagccgcc attgtaaagc aaggactgga
    38281 tccgccaata tcggtattgt ttcccttcct catttcctaa aagtgggctc agcacatctt
    38341 tagaatacgc ttctgctaat tctactgtgt tttctccgcc atgatttttt acataggcaa
    38401 tataatcttt cccaaaatta tatgcttgga cagctgtcca taaatcgcat ccttgtttct
    38461 ttgcttctac tatcatttcc gccaaatatt tgacaccttg atcaatactg tcagctggat
    38521 tatcaatgat ctcagcttct ccatgcagac tttccgagct ctgcatgaga tctgtccctt
    38581 ttccttttga ttcggtcaat attatcgcct tgatcaaatt ctcgtagttt tccatatgat
    38641 aattttttgc agctgttgtt atttctattt gatagctttc cactcgtttt accgaacgat
    38701 aagttcttcc tcataaaaac aacaccgcac caataaccgt cagaatgatc atgtaaaaaa
    38761 gaatacggat aatagataat cgttttcgct tcatttcatc acctagttat tttgacatct
    38821 ttttttatct tactatttct ttatcttctt ggctatttct tccttgattt ctgtgtacgc
    38881 cttaagaaaa aatgaatttt cctgtatttt ttgacatctt acgaaaatga tcgttgtttt
    38941 tcagtaaaaa caattgccca atgactgata taatcgttat actttacaca gtaggtgatt
    39001 atgtgaaaaa agaaaagaaa tatgcaaaaa tgccagatgg gagcgaaatt tactatgaaa
    39061 agagcggtca aggcttccct ctctttttac tgcatggcaa tgacggcagc ggccgctttt
    39121 tttcagaaca ggtccctgta ctcgaacgct actataccgt gtatctagta gatagccgag
    39181 gacatggtcg ttcgacaaac gaagcatcta tgttgaattt tcagttaatg gccgaggatc
    39241 tgaatacgat catgttgctg gaaaaaatcg atcaggcaga ttttcttgga tttagtgatg
    39301 gagcaaatct agcactggta tttgccagca gcttccctaa aaaagttcat cgattgattt
    39361 taaattctgg aaacacgctt gttaaaggcg ttcgtttttc tgccagagtc atcagtaata
    39421 ttcattatgc atgggtatgg ttactctctt tatttcgccc aagtcttcgc aaaaatcttt
    39481 tagttatcaa acttttgctg catgacatcg ggcttacaga aaatgattta aaaaaaatca
    39541 actcccccac tttgatcatc gtgggaaaaa aagatgtaat caagctaaaa cattctttgt
    39601 atatagctaa aactatacca aaagcttcct ttgtgcttgt gaaagaacaa ggacatgaat
    39661 tagctagaaa agatcctgaa cgcttcaatc gagaagttct tcaatttctt tctgaaacat
    39721 aactattagg aatagaaggt gatttatttg ttaaaaaata ccatacaatg gctgaaaaac
    39781 cacctaggac tgttcaaaac aatctttttg atttctgtca ttgtcattat tgtcggtgaa
    39841 ttgatgtcca tcggtaaaac tctatctatc cagcaattag gagaaacgtt tgccactata
    39901 cctgtatgga aaagcggact aatgctattg attggtctta tctctgtgtt acctatggtt
    39961 ggttatgata ttattttgaa tcggatgctt gaacaaaagc agaatcctcg ttacctgttt
    40021 gagacaagct ggctgatcaa tacgatcaac aatattgccg gtttcggcgg gtttgttagt
    40081 gtggggcttc gctctgaact atatggacaa aagaaagaga gcagaaacgt gatccaagca
    40141 ttatcgaaag ttttcttgtt tttgatggct ggactttcta tttacagtct gctttctttt
    40201 ttacttgtag tcttcacgcc agttgctcct tttttgaagc aatattggat atggttgatc
    40261 ggcggcggtc tatattttcc tgtcgtcttt ttatttacta ctttcaaaaa gaaaggattc
    40321 cttggtggta cctcacttaa aactcaagga cagctattgc tagtttcatt gttggaatgg
    40381 accggtgtcc tgtttagttt cctttctgtt ggttatttaa tggaaatccg gattgatctg
    40441 tggcaaacaa ttccattatt cattgctgct tcagtgatag gaatcgtgtc tatgattcca
    40501 ggagaaattg ggagtttcga tgtaatgatg attatcgggt tgtccgctat tggtgtgcca
    40561 agagaaatag tcgttgtgtg gatcttgctc tatcgcctgt tctactacat cattccgttt
    40621 ttgattggaa tcgtcttttt cttcaaaaat atcggttcca cttttgatca gcgttattct
    40681 ggtatcccta aacaattagc aacagaaatt gcgcataaaa tcgtggttgt cttattatac
    40741 ttttctggaa tcatgctagt actttctgcc actatcccgc aagcttttac agagtttcgc
    40801 tggcttcata gtttgaaccc attgaaattt cattttatta tccaatttcc tagtatacta
    40861 ttgggatttc ttttaatcat tatgggcaga gggatcgcag cacgtgtgaa acgagcatat
    40921 ttgcctacaa tcttcttgat tgttctagct ttactttatg ttttattaag tgattttagt
    40981 ttcacaccgg tggttttctt gtctattctg cttctgatca ttcttgcttc taaaaacgaa
    41041 ttatttcggg aacaattgat ctattcatgg gagtggcgta cgatcgatgg aatcattatt
    41101 ggtgcactgt ccttgcttta catcgttatt ggggtataca atctgccgga tttccctcat
    41161 cggcgtcacc attttatttc ctttttctta tttccttctg aaaagatttg gttttcggga
    41221 ttgttggcca tcatcgctgt tagtttcatg atcgttctat ttgttcattt ccttcaagga
    41281 gaaaagaaac agattggcga agcctttaat gaagaaaagg ccctgaagat ccttacaact
    41341 tatggaggaa actccgatag tcaattgatc ttcttaaaag ataagcggat gtttgcctat
    41401 gaaaaagacg gtgaaccaac tgttcttctc caattcgctt gcttcaataa taaatgtatt
    41461 gtaatgggtg atccttctgg taagaaagaa gattttccag aagcaatcga agcttttatc
    41521 gaagaaacag atcgactatg ctatttacca gttttctacg aaacaagcga agagattgtt
    41581 atgattttgc atgaattcgg ctacgatttc attaagatgg gggaagaagc ttatgtagac
    41641 ctgaatagtt ttacaacttc agggaaaaaa atgaagggca ctcgtgctgt cttgaatcgg
    41701 atcgaacgag aaggatttac atttgatgtc ttacaaccac cattttctac tgagcaaatg
    41761 agtactttca aaaatatctc tgataattgg ttgggttcac gaaaggaaaa agggttttct
    41821 ttaggctttt tctctgaaga ttatctacaa agagcgccta ttgcagttgt caaaaatacc
    41881 caagaagaaa ttgtcgcttt tgcaacgatc atgcctactt acacgaacaa taaagtagga
    41941 acgattgatc tgatgcgata tgattctaaa aaagcacctt ctggcagtat ggatttcttg
    42001 ttcttgcatt tgtttgccta tatgaaagaa gaacatatcc aatggttcaa tttagggatg
    42061 gcccctcttt caaatgtcgg aacttccaga aagagttttt tacaggaaag gattgcatac
    42121 ttggtttacg agtttggttc tcatttttat tctttccatg gattaagaga atataaaagc
    42181 aagtacgcta caaactgggt ctcacgttat actctatatt ctagagatag ttggattgca
    42241 tatgttatga ttgctttgct gattattgat aatgcgccag ttgagcagac acaaggtaga
    42301 ataagtggtt tgaaaaaatg gatacgaaga aagtattaaa aactgcatac cgatagatga
    42361 acactctcat gatgaataaa agagactgaa gtaaaacgaa agttcatgct tctacaatct
    42421 cttcttatac aattgaagaa caaagagcgg tccaaacaga aatcctgttt ggaccgctct
    42481 tttattttaa acatttttca ttaccaaaat attcgagtcg cgtttcttat gcttcgatct
    42541 ctgtaacgat acctgaacct actgtccgtc cgccttcacg aattgagaat gtcgttccgt
    42601 tttcaacagc gattggatgg atcaagtcaa catcaatcgt cacgttgtca ccaggcatga
    42661 ccatttctgt atcttctggc aatgtgattg ttcctgtaac atcagttgtg cggaagtaga
    42721 actgtggacg atagttgttg aagaatggag tatgacgtcc accttcttct tttgtcaaca
    42781 cgtacacttc tgctttgaat tttgtatgtg gagtaatgga acctggttta gcaagtactt
    42841 gtccacgttc gatatcgtca cgttggatcc cccgtaacaa cacgcctacg ttatccccag
    42901 cttccccgta atctaacgtt ttacggaaca tttctacacc tgttacaact gctttttgtg
    42961 tttcaggttt gatccctacg atctctacct catcaccgac acgaacagca ccacgatcaa
    43021 tacgacctga ggcaacagtt cctcgacctg taattgagaa gacgtcttcc actggcaata
    43081 gtaatggttt atctgtatca cgttctggtg ttgggatata ttcatctact gtatccatca
    43141 attccataat agcagcttca gcatctggat cgccttgcaa tgcttttaat gcggaacctt
    43201 tgatcacagg agtgtcatcg cctgggaaac catattcgct caataactcg cgaacttcca
    43261 tttctaccaa atcgatcaat tcttcatcat cgactaaatc aactttgttc aagaaaacaa
    43321 tcaggtattt tacaccaact tggcgtgaca ataaaatgtg ttcacgtgtt tgaggcatcg
    43381 gaccgtcagt tgcagaaaca actaagattg caccatccat ttgtgctgcc cctgtaatca
    43441 tgtttttcac atagtccgcg tgtcctggtg catcaatatg cgcgtagtgg cgtttttctg
    43501 tttcatattc tacgtgtgcg gtattgatcg taatcccacg ttcacgttct tctggagctg
    43561 catcaatact agcatagtct tgaggatttg ctaggccttt tttgcctaat actgtagtga
    43621 tcgcagcagt taaagtagtt ttcccatggt caacgtgtcc aatcgtacca atgttaacgt
    43681 gtggtttact tctgtcataa tgttcttttg ccataattta caaacctcct aaataatata
    43741 gatcactgaa aaatttcagc tctcaccttt ttattatagt ctttttttga ctaaatcaaa
    43801 aagaatacgt ccgacttact ttttgtaaga ttcgaactaa gcttattata cgtcaatatt
    43861 ggtcaaagtc aactaagagc actcgtctgt cttatacgcg aaaaaagtac cttttataaa
    43921 taaaaaaatg tgttttttga gaaaaacttc aagatagtcc ttaactttct tttgaagcaa
    43981 taagaacaaa atgaggaaaa gtttaaaatt cagctgttgc caacaacttt caatagacga
    44041 aatcttctat ttcgttaata ataaggaaag aggtgaagta catgaaaaaa ttaattggcg
    44101 tacttgtcgt tttaggcata tttctcggcg gaagttttgc agcttatcat tatttttacg
    44161 gcggcgaagc ttattataca aaaatcacga cgaatggcga aatgtcagct acccaagcag
    44221 ataacggaga gaaattcacg acttatacct acaagcaagc ggcttataat aaagatggtg
    44281 aaaaaaaaca ggtgacgctt cgcgaagaaa gagaacatcc attaaagatg aatgcgtatc
    44341 ttaaactcaa agtcaatcct cgtaaaggtg tattaagctg ggaagaaata aaggcttctg
    44401 aagtgcctaa aaatgcggca gaaaaaatcg ataattagta aatggtctta aaattttatt
    44461 agataaaaag aggctgggac agaagcgttt aactccaaga aata
//
LOCUS       DAZBSA010000011        43443 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000011 DAZBSA010000000
VERSION     DAZBSA010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.011"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            117..2714
                     /gene="adhE"
                     /locus_tag="ACHGMT_000555"
                     /gene_synonym="adhC"
     CDS             117..2714
                     /gene="adhE"
                     /locus_tag="ACHGMT_000555"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFN6834326.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2943..4049
                     /locus_tag="ACHGMT_000556"
     CDS             2943..4049
                     /locus_tag="ACHGMT_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFN6834327.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            4066..5094
                     /locus_tag="ACHGMT_000557"
     CDS             4066..5094
                     /locus_tag="ACHGMT_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFN6834328.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            5100..5579
                     /locus_tag="ACHGMT_000558"
     CDS             5100..5579
                     /locus_tag="ACHGMT_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFN6834329.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5595..6890
                     /locus_tag="ACHGMT_000559"
     CDS             5595..6890
                     /locus_tag="ACHGMT_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFN6834330.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            6901..7725
                     /locus_tag="ACHGMT_000560"
     CDS             6901..7725
                     /locus_tag="ACHGMT_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFN6834331.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            7890..8789
                     /locus_tag="ACHGMT_000561"
     CDS             7890..8789
                     /locus_tag="ACHGMT_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6834332.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(8915..9784)
                     /locus_tag="ACHGMT_000562"
     CDS             complement(8915..9784)
                     /locus_tag="ACHGMT_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFN6834333.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            9937..11397
                     /gene="rhaB"
                     /locus_tag="ACHGMT_000563"
     CDS             9937..11397
                     /gene="rhaB"
                     /locus_tag="ACHGMT_000563"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFN6834334.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            11394..12650
                     /gene="rhaA"
                     /locus_tag="ACHGMT_000564"
     CDS             11394..12650
                     /gene="rhaA"
                     /locus_tag="ACHGMT_000564"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFN6834335.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            12664..13509
                     /gene="rhaD"
                     /locus_tag="ACHGMT_000565"
     CDS             12664..13509
                     /gene="rhaD"
                     /locus_tag="ACHGMT_000565"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFN6834336.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            13511..13822
                     /gene="rhaM"
                     /locus_tag="ACHGMT_000566"
     CDS             13511..13822
                     /gene="rhaM"
                     /locus_tag="ACHGMT_000566"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFN6834337.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            13904..15055
                     /gene="fucO"
                     /locus_tag="ACHGMT_000567"
     CDS             13904..15055
                     /gene="fucO"
                     /locus_tag="ACHGMT_000567"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFN6834338.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            15182..15940
                     /locus_tag="ACHGMT_000568"
     CDS             15182..15940
                     /locus_tag="ACHGMT_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFN6834339.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            16040..16684
                     /locus_tag="ACHGMT_000569"
     CDS             16040..16684
                     /locus_tag="ACHGMT_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFN6834340.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            16691..17698
                     /locus_tag="ACHGMT_000570"
     CDS             16691..17698
                     /locus_tag="ACHGMT_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFN6834341.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            17714..18556
                     /gene="kduI"
                     /locus_tag="ACHGMT_000571"
     CDS             17714..18556
                     /gene="kduI"
                     /locus_tag="ACHGMT_000571"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFN6834342.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            18576..19379
                     /locus_tag="ACHGMT_000572"
     CDS             18576..19379
                     /locus_tag="ACHGMT_000572"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFN6834343.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(19628..20692)
                     /gene="fni"
                     /locus_tag="ACHGMT_000573"
     CDS             complement(19628..20692)
                     /gene="fni"
                     /locus_tag="ACHGMT_000573"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFN6834344.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(20689..21774)
                     /locus_tag="ACHGMT_000574"
     CDS             complement(20689..21774)
                     /locus_tag="ACHGMT_000574"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFN6834345.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(21787..22764)
                     /gene="mvaD"
                     /locus_tag="ACHGMT_000575"
     CDS             complement(21787..22764)
                     /gene="mvaD"
                     /locus_tag="ACHGMT_000575"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFN6834346.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(22757..23701)
                     /gene="mvk"
                     /locus_tag="ACHGMT_000576"
     CDS             complement(22757..23701)
                     /gene="mvk"
                     /locus_tag="ACHGMT_000576"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFN6834347.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            24017..24676
                     /locus_tag="ACHGMT_000577"
     CDS             24017..24676
                     /locus_tag="ACHGMT_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFN6834348.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            24761..25666
                     /locus_tag="ACHGMT_000578"
     CDS             24761..25666
                     /locus_tag="ACHGMT_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFN6834349.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(25668..26300)
                     /locus_tag="ACHGMT_000579"
     CDS             complement(25668..26300)
                     /locus_tag="ACHGMT_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFN6834350.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    26451..26576
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            26620..27144
                     /gene="infC"
                     /locus_tag="ACHGMT_000580"
     CDS             26620..27144
                     /gene="infC"
                     /locus_tag="ACHGMT_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFN6834351.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            27216..27416
                     /gene="rpmI"
                     /locus_tag="ACHGMT_000581"
     CDS             27216..27416
                     /gene="rpmI"
                     /locus_tag="ACHGMT_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFN6834352.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            27469..27828
                     /gene="rplT"
                     /locus_tag="ACHGMT_000582"
     CDS             27469..27828
                     /gene="rplT"
                     /locus_tag="ACHGMT_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFN6834353.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            28080..29417
                     /locus_tag="ACHGMT_000583"
     CDS             28080..29417
                     /locus_tag="ACHGMT_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFN6834354.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            29465..31594
                     /locus_tag="ACHGMT_000584"
     CDS             29465..31594
                     /locus_tag="ACHGMT_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFN6834355.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            31569..32258
                     /locus_tag="ACHGMT_000585"
     CDS             31569..32258
                     /locus_tag="ACHGMT_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834356.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(32666..33343)
                     /locus_tag="ACHGMT_000586"
     CDS             complement(32666..33343)
                     /locus_tag="ACHGMT_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN6834357.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTRFRWLR
                     LTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDYFED
                     IQDRPIK"
     gene            complement(33479..33673)
                     /locus_tag="ACHGMT_000587"
     CDS             complement(33479..33673)
                     /locus_tag="ACHGMT_000587"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834358.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(33723..34163)
                     /locus_tag="ACHGMT_000588"
     CDS             complement(33723..34163)
                     /locus_tag="ACHGMT_000588"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFN6834359.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(34167..35012)
                     /locus_tag="ACHGMT_000589"
     CDS             complement(34167..35012)
                     /locus_tag="ACHGMT_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFN6834360.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            35161..36579
                     /gene="pepV"
                     /locus_tag="ACHGMT_000590"
     CDS             35161..36579
                     /gene="pepV"
                     /locus_tag="ACHGMT_000590"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFN6834361.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(36636..37583)
                     /locus_tag="ACHGMT_000591"
     CDS             complement(36636..37583)
                     /locus_tag="ACHGMT_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN6834362.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(37806..38156)
                     /locus_tag="ACHGMT_000592"
     CDS             complement(37806..38156)
                     /locus_tag="ACHGMT_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFN6834363.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            38356..39435
                     /gene="pepA"
                     /locus_tag="ACHGMT_000593"
     CDS             38356..39435
                     /gene="pepA"
                     /locus_tag="ACHGMT_000593"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFN6834364.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            39579..39899
                     /locus_tag="ACHGMT_000594"
     CDS             39579..39899
                     /locus_tag="ACHGMT_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFN6834365.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            39921..40385
                     /locus_tag="ACHGMT_000595"
     CDS             39921..40385
                     /locus_tag="ACHGMT_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFN6834366.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            40590..41195
                     /gene="ytpR"
                     /locus_tag="ACHGMT_000596"
     CDS             40590..41195
                     /gene="ytpR"
                     /locus_tag="ACHGMT_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFN6834367.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(41234..42523)
                     /locus_tag="ACHGMT_000597"
     CDS             complement(41234..42523)
                     /locus_tag="ACHGMT_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFN6834368.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            42951..43430
                     /gene="rlmH"
                     /locus_tag="ACHGMT_000598"
     CDS             42951..43430
                     /gene="rlmH"
                     /locus_tag="ACHGMT_000598"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFN6834369.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
BASE COUNT        13731 a         7938 c         9308 g        12466 t
ORIGIN      
        1 atttaaaaat aactgtgaaa aaaatcacaa aaaagtgttt acatagaaaa ataaatattg
       61 tatgatatac atgtgaatta tttaacaaat aaccatgacc cattaggagg ataatgatgg
      121 ctaaagtaat agaaaaagaa aaaacgaaga caattgacgt acaagcaatg attgatgatt
      181 tggcagaaaa agcaaatgca gcattgaaag aaatggaaag ctttgatcaa gaaaaagtag
      241 accatatcgt gcatgaaatg gcaatggcag ctttggatca acatatgcca ttagcgaaga
      301 tggctgtaga ggaaacaggc cgcggtgttt acgaagataa agcaatcaaa aatatgtatg
      361 cgtccgaata tatttggcat aatatcaaac atgataaaac agttggtgtg atcaatgaag
      421 atgtccaaaa agggttgatt gaaattgctg aaccagtcgg tgtggtttgc ggagtaactc
      481 caacaacgaa tccgacttcc acaacgattt tcaaatcaat gatcgcgttg aaaacaagaa
      541 atccaatcgt ttttgctttc catccaagtg cccaaaaatc ttctgcagaa gcagcacgag
      601 tggtacgtga tgctgctatc gctgccggag ctcctgaaaa ttgtatccaa tggatcgaac
      661 atccttcgat cgaagcgact tctatgctaa tgaaccatcc aggtatcgcg atcgtcttag
      721 cgacaggtgg agctggaatg gtcaagtctg cttactctac aggaaaacca gctttaggtg
      781 taggtccagg taacgtacca gcttatattg aaaaaacagc aaaaattaaa cgtgcagtga
      841 acgatttgat cgtttcgaaa acatttgata atggaatgat ttgtgcttct gaacaagctg
      901 tgatcgtgga taaagaaatc tatgcagcag taaaagcaga attccaagca caccaagttt
      961 atatcgtaaa accagatgaa ttgcaaaaat tagaagatgc agtgatgaac gaaggaaaat
     1021 atgcggtcaa tccatcaatc gttggacatt ctgcgatgga aatcgcaaaa ttggcaggaa
     1081 tcagtgtacc aaaaggaacg aaaatgctga ttgctgaatt ggaaggcgta ggcccagatt
     1141 atccgctatc aagagaaaaa ctttctccag ttcttgccat gatcaaagct aacaacacgg
     1201 accatgcgtt tgatttgtgt gaaggtatgt tgaatcttgg aggattagga catacagcag
     1261 ttatccattc agaaaacgaa gagttgcatg tcaaattcgg tcttcgaatg aaagcttgtc
     1321 gtattttagt gaatacacca tctgcagaag gcggcatcgg tgatatctat aacgaaatga
     1381 ttccgtcatt gactttggga tgcggttctt atggtaagaa ctctgtttct cgtaacgtct
     1441 ctgctgtaaa cttaatcaat gtcaaaactg tagcgaaacg gagaaataac atgcaatggt
     1501 tcaaattacc gccaaaaatc ttctttgaaa agaactctct attgtactta gagaaaatgg
     1561 aaaacgttga acgtgtcatg atcgtttgtg accctggtat ggtccaattc ggttactgcg
     1621 atacagttcg tgaagtattg gcacgacgta aaaatgatgt gaagatcgaa gtcttctcag
     1681 atgttgaacc gaacccatcg acaaataccg tatacgcagg aacgaaactg atggcagact
     1741 tcaagccaga tacagtaatc gctcttggag gcggttctgc aatggacgca gctaagggaa
     1801 tgtggatgtt ctatgaacac ccagacacat ctttctttgg tgcaaaacaa aaattcttgg
     1861 atatccgtaa acggacatat aagatcgaca aaccagaaaa aacacaattc gtatgtatcc
     1921 cgacaacatc agggactggt tctgaagtaa cgccatttgc ggtcatcact gacagtgaaa
     1981 cacatgtgaa atatccgtta gcagattatg cattgacgcc agatgtcgca atcgtcgatc
     2041 ctcaattcgt catgtcagtt ccagcatctg tcacagcaga taccgggatg gatgtgttaa
     2101 cacatgcaat cgaatcctat gtttcagtca tggcttcaga ttatacacgt ggattgagcc
     2161 tacaagcaat caagttagtc tttgactatt tagaaaaatc agtaaaaaca cctgatatgg
     2221 aatcacgtga aaaaatgcac aacgcttcta caatggctgg tatggcgttt gctaacgcat
     2281 tcctaggtat ttgtcactca gtcgcacata aaatcggcgg ggaatacggg atccctcatg
     2341 gacggacaaa tgcgattctt ttaccgcata ttatccgcta taatgcaaaa gatccacaaa
     2401 aacatgcgat gttccctaaa tatgactatt tccgtgcaga tacagattac gcagatatcg
     2461 caaaattctt aggtctgaaa ggcgaaacaa cagaggaatt agtcgaagct ttagcaacgg
     2521 ctgtttatga attaggaaaa tctgtcggca tcaacatgag tctaaaagct caaggagtca
     2581 cacaagaaac actagataca accgtagacc gtatggctga attagcttac gaagaccaat
     2641 gtacgacagc taatccgaaa gaaccattga tctctgaatt gaaacagatc atcattgatg
     2701 cttacaacgg ataagcaaaa gaaagcgtag aaaaaagata agaaaataga tgaaaaagaa
     2761 cttggaaaac gttgtttctc caagttcttt ttttaatttc caagtaactg aaagttactt
     2821 ttgttattta acatttaggt caattttaga cagtttttgt tcaaaaagcg atatttatag
     2881 tggacggtat tactgctata ttaaggatat aaaatgaaag cgtttaaaaa ggtggcgtaa
     2941 agatgattat taaagaagag atttttgtac ctaaagaatt agtgaaagaa aaaattgacc
     3001 tgctcgttga aaacctgaca acgatcaaag acgataatgg tgaatttttg ttggactttg
     3061 atggactgaa agtagatgac aaaagctgga cagtgtggaa ttggccacaa ggtgtcggtc
     3121 tatatggtat ttataaaaat taccagatga caaaaaatcc tcgcgcttat caagtggtca
     3181 atgaatggtt tgaggatcgt atggaagaag gcgctccgcc caaaaacgtc aatacaatgg
     3241 caccgctatt gactatggct tacttgtatg aagacacgaa agattcacgc tatttgcctt
     3301 atttagaaca atgggcagaa tgggtcatga atgacatgcc tcggacaaat gaagacgggt
     3361 tacagcatgc cacatatgga ccggaaaata agaatcagtt gtgggacgat acattgatga
     3421 tgacagtgct tcctttggca aaaatcggga aactgttgaa tcgcccagaa tatttagaag
     3481 aagctcgtta ccagtttatg atccatatca aatacttgat ggataaacga acaggattgt
     3541 ggtatcacgg ctggacgttt gaaggaaatc ataactatgc cgaagcattt tgggctcgtg
     3601 gaaactgctg gttgacgatt gctattccag agatcattga aatcttagaa ttatcgaaag
     3661 aagatgcatt gcgcaaatta ttgatcgaaa cacttgaagc acaagtgcgt gcattaaaaa
     3721 cttatcagtc tgaaagcggt ttatggcata cgttgttgga tgatccttct tcttacgtag
     3781 aatcttctgc aactgcagga tttgcttacg gtattttgaa agcggtccat aaacgttatt
     3841 taccacaaga atataaagaa gtcgcttatc gtgctatcca aggattgctt gaacaaatcg
     3901 atgaaaaagg agaagttcaa aacgtttcaa tcgggacagg aatgggggac agtttagatt
     3961 tttatcggaa tatcggaatc acagcaatgc cttatggaca gtcgttaacg gtgctttgtt
     4021 tgacagaatt gctcgtctct tattgttaaa agaaaggaaa cccatatgaa aaaaacatca
     4081 ctgctgtttg cagcaagttc gcttagccta ggtctgctag gtctaagcgg ctgcggaaat
     4141 aattctagtg tggcaactgt cgcagatgca gatgtaaatg agatagttac cttgcgcttt
     4201 gcttatgcaa gcaacagcca gccagtaatt gatgcaatga atgaatttgg gcgtttagta
     4261 aaagaaaaaa cagatggttc cgtacagatc cagtatttcc cagatggaca actgggtgga
     4321 gaacgtgaac tgatcgaatt gacacagtca ggagcagtag atttcacaaa agtaagtgcc
     4381 tcagcgttgg aaagtttctc taaagattat tcgatttttt cagttcctta tctattcaat
     4441 gatgaagaac atttctttaa ggtgatggat aatgaagaaa taatggagcc aatctaccaa
     4501 tcgaccaaaa atttaggatt taccggattg acctattatg attctggtca gcgcagcttt
     4561 tatatggtag acggtccgat ccatacgccg gaagatttga agggaaagaa gatccgtgtg
     4621 atgcaaagcg aaaccgctat caaaatggta gagcttttag gcggttcgcc cgtacctatg
     4681 ggaagtgatg aagtgtacac gtctcttcaa tccaatttga tcaacggatc agaaaacaat
     4741 gaatttgtct tgtatacagc tggacatggt ggtgtggcta aatactattc ctatgatgag
     4801 catacacgtg tgccagatat catcatcatg aatgatgcca tcaaagaaca gctaactgat
     4861 gagcaggaaa aagccattga agaagcagcg aaagaatcaa cggtttttga aattgaagct
     4921 tttgcaaaag caattgaaga agaaaaagct ttagcacaag aagaacatgg agtccaattc
     4981 aatgatgtag ataatacacc gtttcgagag gcagttgccc cacttcatga tatattcaaa
     5041 aacgatccta cctacaaaga cctttatcaa aagatccaag agcaaggagc gtagctgtga
     5101 tgaaaatgat tttaaacaag gtattagaga tcctgggagc tgttttgctt gttgggatgg
     5161 tcgctgttgt tttatggcaa gtattttcca gaacggtttt aggaaatcca aatacggtca
     5221 cggaagagct tgttcgattc ggtcttgtct ggtttgccat gttgtcttcc gcgtatgttg
     5281 tgggacaaaa agcacattta gccgtaacgg ttttaagtga aaaattaact ggcaaaaaaa
     5341 gtaaaatttt agaagttgtt gttcaaagtt tatttctggt atttgctgcg atgatcatgg
     5401 tgtatggcgg atggaatgct gtcacattga caatggggca gatctcgcct tctttaagta
     5461 ttcctatggg atatgtttat ctgtcagtac ctgtatcggg ggtactcatc attatctata
     5521 gcctgatcaa tctaatcgat acattaagca acaagccagc aattcaagaa aaaatataaa
     5581 aatggaggaa taaaatggca atagaagccg gattagtgtt attttttgta tttgttgtgt
     5641 tgcttgtatt agggatgccg attgctataa gtattgcatt ctcatcgatg atgactttat
     5701 tgttagttat accgtttgat gtttctgctt ttagttcagc tcaaaaaatg gtgggaagta
     5761 tcaatagttt ttcgctgatc gctattccgt ttttcgttct ctcgggcatt atcatgaata
     5821 atggagggat agctaagaaa ctagtggatt ttgcgatgtt gttcgtcggg cgcgtgccgg
     5881 gggctttggc tcatacgaat gtgttaggga atgctttatt tggttccatc tctagctcgg
     5941 ccattgccgc ttctaccgcg attggcggag tgttgatccc tcagcaagtg gaagaaggat
     6001 atgaccggaa gtttgcgaca gcggtcaata tcgcgtcagc tccgacaggt atggtcattc
     6061 ctccaagtac tgcttttatc atgtattcac ttgttgctgg tggtgcttcg atctctgctt
     6121 tgtttatggg aggttatctc gtaggcgctt tgtgggcgct aggaatcatg ctagtagcat
     6181 tcatcgttgc taaaaagaat aaataccctg ttgtcgaaaa aggacagatg gctcatgctg
     6241 gaaaaataat aaaagaggcg attcctagcc ttctattgat tgtcatcatt atcggtggta
     6301 tcttgacggg gatatttaca gcagtagaag cttcagcgat agctgtcatt tactccctgt
     6361 tgattgctat gttttactat aaaacggtaa aactaaaaga tatacctaat atgttgtttc
     6421 aatcagtagc aatgtcaggg acgatcatgt tcttattggc tacttcttcc atgatgtctt
     6481 tcgccatggc gtttaccggt attcctcagg caattagttc gttgattatg ggcgtaacgg
     6541 ataataaatt tattattctg ttattagtca atcttgtgtt attactggtg ggtatgttta
     6601 tggatgtggc gcctgctatc ttgattttta caccaatatt tttaccaatc gtcacaagtg
     6661 taggggttga tcctgtacat tacgggttgt tttcaatcat gaatctgtgt gtaggctcca
     6721 tcacgccgcc agtaggtaca ggactatatg tcggtgcaag tgtaggaggc gtaaaagcag
     6781 aggcaatgct gaaaccgctt cttccttttt atggggttat cttagtcgtc ttgtttttga
     6841 ttacttactt cccgcaaatc gtcatgtggc tacccaatat gctgagttag gagaatagtt
     6901 atgttgaaca ttacaattag aggacatgat ctttccgaag tccaaacgat agaagatctt
     6961 gcagaaaaaa caaaagaaca agggattcat acactgcagc tcgccttagg tctttcgttt
     7021 cctgaaatac cttctggcgc aaatgagatc aattctggga tgggaaatta tgtcaaacga
     7081 gttttagaaa agcaagaagt gaccgtagga attttaagtt gctatatcaa tatgattcat
     7141 ccagatttaa cgatacgaca agagttgctg acaaagttcg agcagtatct tcgctatgct
     7201 tcttcctttg gtgcgtcgat ggttgcttcg gaaactggat gtgttttgcc ggaaatccaa
     7261 tatacggaag aaaatttcac agatgaagca tttgctgaag ctgtgtccgt tatccgtcgc
     7321 ttggtaaaag ctggagaaaa ataccaaatg atggtgggga tagaaccagg tttgaatcat
     7381 ccggtgtatt ctttagcaag ggtagagcag ctgatccaag cagtagattc ggactatcta
     7441 ggaatcattt tagatccgac caatttgatc acaagtagtg attatcaaga acaagtccaa
     7501 ttggtagaag aagcttttga acgttttggt gaaaagatat gtgcggttca tttgaaagat
     7561 tttcgtgtcg aacaggaaaa aatcgtgccg gtaaatttgg gagacggtgt gatcgagtac
     7621 acaaaaatca aagaaatcat catgaagaat cgtccgtacc tgtatgtggt tcttgaagaa
     7681 acaaaagatg atgggattcg ttatggaaga agtcttcttg aataatgtaa agaagacgtt
     7741 ggttaactaa tatttagaat ggagggggcg gggctagaag atgccacgtc tcttcttttt
     7801 tataagagta tataaaagca gctctttttg attacaaaaa acgtggtata ataaaattgt
     7861 gcataagtga acaaaaggag gtgcggcaca tgctccaaaa gaaaatcagc ggtgaacaag
     7921 tcaatacgat ggtttctgat cagtatgaaa tctatcatgt caaagatgtc gtcagccaaa
     7981 ataaaacgat ctatcatcat catgatttct atgagatcca tgctacatta aagggagaag
     8041 cgacttttta tttgaacgga cgacaattta cgatcaaagc aggaagtgtg ctgctcatcc
     8101 attccaatga tttgcatcgt atcgtccgcc aatcttcaga tgtattcgag cgtgtctata
     8161 tctttgtcac cccttctttt ctagaaagtc gttccacgcc atggtctaat ctatcggctt
     8221 gttttcaacc aatgggagag aagaggagtc gtgtattgca aatagaacag gaagtattga
     8281 agcaaaaatt atcatttatc gaccatcctc ctgataaaaa aatttacggt gaagacataa
     8341 aatatgaaca agctctggta gaatacttga tttttctcaa tcaagttgta caaaaagaag
     8401 agagtgcgca agaacagaat gctgtagttc ctaatgagcg tgtggaacaa atgttacaat
     8461 atatctcaaa aaatttatct catccgcttt ctttagagga aatggaaaag cagttttttg
     8521 tcagcaaata ccatatcaca cgagaattta aaaagtacac tggatttacg tttcatcagt
     8581 atgtaatgaa gaaaaagctg atctatgcca agcacttgtt gcgagaatac cgcagctcta
     8641 gtgctgtata tagtaaaagt ggtttttctt cttatccgca ttttctgaaa gcattcaaga
     8701 gagagttcgg cataacgcct aaagaatttt tgaaacgtga tacacagaaa cagttggttc
     8761 attatgaaca ttttgaagat gaacaatgag aatgatcact aaaaaataac cgcagtagct
     8821 gctgttgttt acatagaagc agaaactgcg gttattttat ttttatctat atatttcaaa
     8881 atattttgaa aaaatgacgt acatattata tgaatcagct gaatttacga aagtctctcg
     8941 gcgatagtcc tgtttttgct ttgaacaggc ggctaaagta agcaaaattc tcaatcccta
     9001 tttcttcgga aatcattcga accgtaaatt ctgtataccg aagaaggtct ttagcgtaag
     9061 aaagtttttt gctgatcaaa taatcgattg gtggagaacc gataaatttg gaaaaatctt
     9121 tgctcagctg atatttattc aataagaatt gttgttctaa ttgatcaagt gtaatcgttt
     9181 ctttgaaatg ttcgtctaag tattgcttca gtttgtaaac gtaggcggga atatcctctt
     9241 cttcgaaatc tagctgatac ttttgcaaaa tcagttcatt cagcaactcg tggatagcta
     9301 cagaggactg gaaatcggta cgggcattcg tgccttgttg catgtggatc aattctgtga
     9361 tcaaatggtg gatcttgttt tctgatggat ctcctgttga atggaaggtg ttatggccgt
     9421 cttttgtgaa ctcctgataa aatgattgga cgttctgacc attcaaatgg atccaatcca
     9481 tttcccatgg ttcttcgctg atcgtttgat aacgctgata ttcttggcag tcaataaaaa
     9541 ataagtctcc agcttttagc tgttgctgat tatcattata tgtaagcgaa cctttgccag
     9601 acaaagtaaa ctttatcaag taagaaggta aatgtgctcg ttcagtatag taagggattg
     9661 gtgctttgaa gtaaccgatt tcttggatat aaaaaaataa attcctgctt ctttgtgtag
     9721 gcgtcaagat cactcttttt gagtcttccg accatgagtg gttggctttt tcccagtaat
     9781 ccatgtaaac actcccaact tgtcaagata atgcaattat ataccaaaat agtgtgaagc
     9841 aaaatataag attctgtatt atgatagcat tgtcaataaa tgataacgct ttattaataa
     9901 agtgctaatc aacaataaag taaaagaggt ggagaaatga atccaacatt tgttgccatc
     9961 gatatcggtg catcaagcgg acgactaatg aaaagtacga gggatgcaaa aggttctttg
    10021 atcttggaag aagtacaccg gttcaaaaac ggctttcgga acaaagacgg tcacgatcgc
    10081 tggaagatcg attatcttat caaagagatt ttgattggat ttgaaaaatt aaagcagaca
    10141 gggattgatc gcctttttgc ggggatcgat acatgggcag tcgattactg tctgattgat
    10201 gaacaagggg atcggataaa tgatccgatt gcttatcggg acaagcgtac caatggagca
    10261 gtagaacagt tttctgaaat ctattctcta gataagttgt atgagcagac agggattcag
    10321 atccagcctt tcaatacctt gttccaattg tttgctgaag aaaaagaaaa tttggcaaaa
    10381 gcacataaat tgttactgat acctgattat ttagggtatg tctttactgg taacatggtg
    10441 acggagaaaa ctaatgcatc tactatgcag ctgctgaatg cagggacaaa agaatgggaa
    10501 atagaacttc ttgaaaaaat cggtgtctcg aaggaattat ttccaccatt gacagagccg
    10561 ggaacgatac taggagatct gcaaaaagaa caattttcaa aatatgattt gccagatgtg
    10621 acctttatca cagtagcaag tcatgatacg gcgtcagctg tattaggaac gccaggtcaa
    10681 ggagaacgtt ggggttatat cagcagtggg acatggtcgc ttttagggat tgaaacaaag
    10741 atcgccaatg tttctcgcaa tgctttttct gaaaattata cgaatgaatg gggtgcccat
    10801 aacacggtgc gttttttgaa aaacatcatg gggatgtggt tgatccagga agttgcccga
    10861 atgcaagagt acgagtacag ttatgcggaa ttggcagagc ttgctgcaaa agaaaaaccg
    10921 ctgcagcaag tgatggaaat caatgaccct cgatttttga atcccccaaa tatgatagaa
    10981 gagattcagc aatactgttt tgaaacagag cagccagtcc ctgtctcatc gggagctatc
    11041 gctcgttgtg tgtatgataa tcttgctctt tgttacgcac aagagctgat caaagtagaa
    11101 cacttaactg gaacgaaatt ggaaaagctg cacatcgtag ggggcggatc gaataatcgt
    11161 ctactgaatc aattgacagc agattatgcc ggaatcattg tagaagcagg acctagtgaa
    11221 gccacagcaa tcgggaatgt tgccatgcaa atgctcagta caggtcagtt ttcgaatata
    11281 gaacaagcaa gaaatgtaat caaacagtcg tttccatgtg aaacttttta cccagatgaa
    11341 aataagcaaa acacgatgga atggtacgtg aataaaatca aaggagtaat cgtatgaaca
    11401 cgattgaaag aaattacgaa caagcaaaag aaaaatatgc aactatcggt gtagatacag
    11461 atgctgtact agaaaagata caggacatca aaatttctat gcattgctgg caaggagatg
    11521 atgtcaaagg tttcttgaca ccggatggcg aattgaccgg tggaatcatg gcgacaggga
    11581 attttccagg agccgcacga acacctgaag agcttagaca agatctggag aaggcttatt
    11641 cgttgatacc gggcaaacac aaattgaacc ttcatgctat ctatttggat acagaagaat
    11701 ctgtcgatct aaatgaaatt gagccgaaac attttgaaaa atgggtagag tgggcaaaaa
    11761 aagaagaaat cggtcttgac ttcaatccga ctttcttttc gcatccaatg atgaaagacg
    11821 gctttacatt ggctcatcca gataaagaag ttcgtgaata ctggatcgaa cacgggaaaa
    11881 gaagccgcaa aatcgcggag tactttggga aagaaactgg gcaaacaagt atcaataact
    11941 tttgggtacc tgatgggatg aaagataatc cgattgatcg ttttacccca cgggaacgtt
    12001 taatggaatc actcgatgaa atttttacag aagagttaga tgaacggtat gtacaagatg
    12061 cagtagaaag caaactattt ggtttaggcg ctgaagctta tacagtcggt tcccatgaat
    12121 tttatatggg ttacgggttg actcgtgaca aacttgtttg tctagatgct ggacattttc
    12181 atccaacaga agtgatttcc aataaattat cttcattagc tttattcagc aaaggaatca
    12241 tgttgcatgt ttcccgtcca gtgcgttggg attcagacca tgtggttttg atggatgatg
    12301 aattgcagga aatcgctaaa gaattagtcc gtaacgaatt gttggaaaaa acaaatatcg
    12361 gtctggattt ctttgatgca acgatcaacc gtatcgctgc ttgggtcatc ggtacgagaa
    12421 atacacaaaa agcactgctg aaagctatgc tggaaccagt tgagcgattg aaagaaatgg
    12481 agctggcttt tgactttact tcaagaatgg cttatacgga agaattgaaa gatttcccgt
    12541 atgccgatgt atggaattat ttctgttaca aaaatcaagt tccagttggt ttggattggc
    12601 tggaagaagt ccaggaatat caaaaagacg tgttggaatt aagaaaatag gagtgtggct
    12661 atattgaaaa aaatagatat tttacaagcg ccttatgtga aagaaatgat cgaaacgacg
    12721 tcgaatcttt atcgcttggg atgggatgaa agaaatggcg ggaatatttc ttatttacta
    12781 aaagaagaag agatcacacc gttcttggat cctgaaaaag tgatccgccg catctcgatg
    12841 atttttgatg ccagccgttt agcaggatgc tattttattg taactggttc aggaagatat
    12901 tttaaaaatg tagcgagcga acctgccgag aatcttggaa tcgttcgagt tgctgaagat
    12961 ggcaaaacac tggaactttt gtgggggctg gaaaacgaat gccttcctac aagcgagttg
    13021 ccaagtcatt tcatgagtca tatcgcccgt ttagaagtag acccagataa tcggatcgtt
    13081 atgcattgcc atgctagtca tctattagcg atgagtttta cgcatgaatt agacgaacgt
    13141 tcctttagtc gtacgctatg gcaaatgtgt acggaatgct tagttgtttt tccagaagga
    13201 atttccatca ttccgtggat ggttccggga acaaatgaga ttggggaagc aacagctgaa
    13261 aaaatgaaag aaactcgatt ggttttatgg ccacaacatg gaatctacgg gtcaggaaaa
    13321 gacatggatg aagtctttgg tttgatcgag acagcagaaa aggcagcaga agtctatacg
    13381 tacgtcaaag cgcaaggacc aatcttgcaa acaatcacgg acgaaaacct gtggcattta
    13441 gcagatgcgt ttggtgtcac tccaaaagca ggttatttgg aggaagttca tacgaaagca
    13501 ggtgtctgac atggtgaaaa aagctttttg catgaaagtt tacccagaaa aacaagaaga
    13561 atacaaaaaa agacatgatg aactttggcc agaaatgcgt acagcacttc gtgaacacgg
    13621 ggtcatcgac tattcgatct atcttgatcc gagtacaagt ttgttatttg gttacttagt
    13681 gatcgaagat gaaaagttat gggaaaaaat ggcaaataca tctatcaatc aaaaatggtg
    13741 gaagtacatg gaagatatca tggagacaaa tccagatgcc tctccagtca gttgggaatt
    13801 gatgccagtc tttgaactgt agcaacaagc aactgggcaa ccggttgctt ggggttcttt
    13861 cgtttttatt gtgaaaaata atacaaatta ggaggaatag aaaatgagta atcgaatgat
    13921 tttaaatgaa acatcgtatt ttggaaaagg agcgattgag tcgatcgtcc ctgaatttca
    13981 gcagcgagga ttcggcaaag cagctgtcat tactgacaaa ggattgatcg agcacggtat
    14041 agcaacaaaa gtaacagaac ttctagataa agcagcaatc gcttatgctt tatacgacgg
    14101 gatcgtacct aatccaacga tccaaaatgt aaaagatgga gtggcttttg ttaaagaggc
    14161 agaggcagac tgtttgatcg caatcggtgg aggttcgcca atcgatacgg caaaagccat
    14221 tgggattatt ttgacgaatc cagagttttc agacgtcatt agtttagaag gtgttgctga
    14281 tacgaaaaat ccatgtttac ctatattagc tataccaacg acttctggaa cagcagcaga
    14341 agtaacgatc aactacgtca tcacagatga agtaaatcac cgaaaatttg tttgtgttga
    14401 tcctcatgat atcccaatcg tcgcgttcat cgacagcgat atgatgatgg gaatgccgaa
    14461 aaaattggct gcatcaacag gtatggatgc tatgacgcat gctattgaag ggtttatcac
    14521 aaaaggcgct tgggaaatga ctgatatgct gcatttaaaa gcaatcgaaa tcatcggcca
    14581 ttctttggaa gcttctgtag atggcgatca aaatgggcga gaaaaaatgg cattaggaca
    14641 gtatattgcg ggaatgggct tctcaaatgt cggtttagga ctggtacacg ggatggctca
    14701 cccgttaagt gcatggtaca acattcctca tggcgtagcg tgtgcagcac ttttgccaac
    14761 tgtcatgaaa tataacaaag aatatactgg agaaaagtac cgtgagatcg ctcttgttct
    14821 agggattaaa ggagcagctg aaatgtcttt agaagatgta agagaagcgg cttgtggtga
    14881 gatcgatcgg ttgagcaaag cggttggaat cccagaaacc atttctgaat taggtgtaaa
    14941 agaagcagat attccagcaa ttgcagaaga tgctttgcga gatgtatgca caccaggaaa
    15001 tccaagagaa acgacagtag aagaaatcat tgctttatat caaagtttga tgtaaaaacg
    15061 aagcggatct tcaacattaa attgcctgta aagagaaatg gtagtttata tctggatatc
    15121 tcttttaaat tgatggtatt tccgctataa tttttcttat aaagaaatag gagaatatta
    15181 tatggaaaat aaaaagcctt atggaactgt attgatcaaa gcctctaaaa tactagacta
    15241 tttagcggaa aaccaagatg tctctttgca gacgatcgca aagggtgtgg gaatgacctc
    15301 ttctacagtc ttgaaaatac tagatacttt gttgatgatc ggctacgtaa ataaaaattc
    15361 agagaaaaac tatcgtttag gtgcaaagct tattcgctac gcgaataaaa acattgaaca
    15421 aatcgatttg gtagagttga ctttaccgtt tttagaacag ctgcagcaaa agatcgatga
    15481 gactatccac ttaggggtat tggacaacaa tgagatcttg tacgtgaaca aactagatcc
    15541 aaaacatcaa acgatccgca tgtcttcaaa aatcggaatc acccgaccat tatacaattc
    15601 tgcgatgggc aaagccgtat tagcagaatt cagtgaagag cagtacactg attatcttga
    15661 aaagcatccg ttgatccctt acacagaata tacgatcacc aatgcattac ggttgaaaaa
    15721 ggaaatcaaa acggtaaaag aaacccacat cgcatttgac gatgaagaaa tcgaacgtga
    15781 tattttttgt ataggagcct caatcgtgaa aaacgaacaa atcattggtg catttagtgt
    15841 aagtatgccg aaatatcgtt taacagatga gacaaaagaa caaataatca gtgccctcaa
    15901 gcaaacaaaa agcgaaatcg agcaaagaat tcaaaaataa tgaaaaaagc gtttgcaatt
    15961 tttcttttct gccgtataat atgggtatag atttccaagt tataaaataa aatttctaaa
    16021 taggaaatag gtggagaaga tgaaaaaaat tgatatttta tctcgattga aaaacgccgg
    16081 agtgattgcg gtggtacgcg gaaaaagcaa agaagaagct ttgaatgctt gccacgcaat
    16141 catcaaaggt ggtctaactg gtattgagtt gacttttaca gtccctcaag cagatcaagt
    16201 aatcaaagaa cttttgtcat tctacaaaga ccaaccagaa atagtgattg gcgcaggcac
    16261 agtattagac gctgtcacag ctcgcttggc tatcttagct ggcgcagagt atattgttag
    16321 cccttccttt gatcaagaaa cggcagagat gtgcaatctg tatcaaattc cttacttacc
    16381 aggatgtatg actattaccg agatcaaaac agcgctgaaa agcggggtag atatcgtcaa
    16441 actatttcct ggaagcgctt atggaccaag tatcatttct gccttcaagg cgccgatgcc
    16501 tcaagtcaat atcatgccaa ctggcggtgt gagtctggac aatatgaaag agtggttcga
    16561 tgctggtgtc gtaacagtcg gagtaggtgg gaacctttta gcacccgcag ctacaggaga
    16621 ttttgacaaa gtaacggaag tcgcgcaaca gtatgcagca aaaatgaaag aaatcaagag
    16681 gtagctgctt atgggaaaag tagtgacact cggagaaatc atgttgcgat tgtccacaga
    16741 gcagggaacc cgtatcgatt caagtgaacg attcgctgcc cattatggcg gaggggaagc
    16801 aaacgtagca atttctttag ctaattacgg acatgaagcg atttttgcta caaaagtacc
    16861 tgataacgca ttgggaaaag cagtcaaaaa gcacttgcaa tgttatggag tagatacgaa
    16921 acaggtatta tctggtggat caagactagg tacttattat atggaaactg gtagtggcga
    16981 acgcgccgcc tcagtgatct atgatcgtgc cggatcaagt tttgctgaaa tggatcattt
    17041 ggaatggtcg ctagaggagt tattccaagg aatagatata tttcatgttt caggaatcac
    17101 cccagctttg tctaaaaaat ggcaaaaaat gacgaaacag gtgatagaag ctgcaaaaaa
    17161 agctggctgc ctgatcagct tcgatatcaa ctatcgtgga aaattatgga cacagcaaga
    17221 agccagcgaa gtaatccatc agcttttacc tttagtagat atctgctcag ctggaaaaat
    17281 ggatgctctt tatttgttag gtatcgagaa agcgccagaa gcagaagaac aacctctaat
    17341 ttattattat caaaaaatgc aggaaaagtt cccgaatatt caggtgtttt attcgaccaa
    17401 gcgtaccgtt cattcagcaa gtgaaaatga attgaagggg acattgtgga tgaaccatga
    17461 atatgtggaa tcccaagtcc atagaatcac cccaatcgtg gatcgagtag gtggcggaga
    17521 tgcttttgca ggtggcgtgc tccatgggat tttatccaat atgtctccgc aagaaatcat
    17581 tgattttgct acggcagctt ctgccttgaa gcatacgatc catggggact gcaaccaatt
    17641 tagtcaagaa gaagtcgcac aatttttagc atgcggctct ggaaaaatca ttcgatagaa
    17701 ggagtagaag aaaatgcaag aaatggaaac acgttataca cacagtccag aagatatccg
    17761 tcattattca acggaacaat tgagaaaaga atttttagta gaaaaggtat ttgaaccagg
    17821 gaaaatcagt ttgacttata cgcacaacga tcgaatgatc tttggtggag tgacacctac
    17881 tgaaaaagaa ttagaaatca cattagacaa agaactaggt gttgattact tcttagagcg
    17941 tcgtgaacta ggtgtgatca atatcggtgg accaggattc atcgagatcg atgggcaaaa
    18001 agaagaaatg aaaaaacaag acggatacta tatcggaaaa gaaacacgac atgttgtatt
    18061 ttcttctcaa gatgctgaaa acccagctaa attctatatt agttctgttc cagctcatca
    18121 taaatatcca aatgtaaaaa tcagtatcga ccaagtaaaa ccaatggaga ctggtgaagg
    18181 cctgacactg aatgaacgta aaatctacca atacatccat ccaaatgtat gcgaaagctg
    18241 tcaattacaa atgggttata cgattttaga accaggaagc gcttggaata cgatgccttg
    18301 tcatacacat gaacgtcgta tggaagctta tgtctacttt gattatgcca atgaagatac
    18361 acgtgtgttc cacatgatgg gcaaaccaga tgaaacaaaa catttagttg tagacaatga
    18421 acaagccatt atttcgccaa gctggtcgat ccattcaggt gtaggtacaa gcaattactc
    18481 attcatctgg gcaatgtgcg gagaaaatat cacctataca gacatggata tggtgcaaat
    18541 ggatcaatta aaataagcat aagggagaga aaatgatgga atttagtatg gatatgtttc
    18601 gtttagatgg aaaagtagca ttagtcaccg gtgctgtcta tggtattgga tttgagattg
    18661 cacgctcttt agctacagca ggagcaaaaa tcgtatttaa taacttgaat caagaatcag
    18721 ttgatgaagg tatagcgcat tataaagaag caggcatcga tgcaaaaggt tatgtctgcg
    18781 atgtgaccga tgaagaagcg gttcaagcga tggttcaaca gatcaaagaa gatgtaggtt
    18841 cagttgatat tctagtcaac aatgcaggga tcatcaaacg gacaccgatg atcgaaatgg
    18901 atgcggcaga cttccgccaa gtaatcgacg tggatttaaa tgccccattc atcgtttcaa
    18961 aagcagttat tccggatatg atcgaaaaag gcggcggtaa aatcatcaat atctgctcca
    19021 tgatgagtga attaggacgc gaaacggtca gtgcttatgc tgcggcaaaa ggcggattaa
    19081 aaatgctgac gaaaaacatc gcttctgaat acggtcaata caatatccaa tgtaatggaa
    19141 ttggaccagg ttatattgct acaccgcaaa ctgctccatt acgcgaaaca caagaaaacg
    19201 gtgaacgtca tccattcgat caatttatcg ttggacgtac gccagcagca cgctggggcg
    19261 agccaatcga tttggctgga ccatctgtct tcttggcttc tagcgcatct gatttcgtta
    19321 acggccatgt cttgtatgtg gacggaggca tacttgccta tatcggaaaa caaccttaaa
    19381 agagctttag tacactgttt agtggagagg ttgtgaaatc agcggattga acgaataccg
    19441 aaggacagct gattaaacat catttattcg aaaaaagagg ctgggacaga agcatttaac
    19501 tccaagaaat aagaaggaat tcacgaaaat tgcttttcaa atttttgtga atttcagctt
    19561 atttccgaag gagttgcttt tacttccgcc gtttgtacgt gtttagagcg tgagacaaaa
    19621 gtgttcttta cttttgtccc acgctctttt ctgtgcgtct tccatacttc actaagtcga
    19681 tccctcttgc ttcgcaccaa tccttcactg gtccggaaaa gatcagagat tgttggtgta
    19741 gggccgcggt attcgttgcg ccgaccatag tataaagcag acgaagctct tcttgccatt
    19801 gcttcatcag tataatagtt tcttctacgc cgtgattcat cagatgcgtc agtacggttc
    19861 ctgaagtacc cacggcattt gcgccgagac aaagggcttt aaatatgtca taggcattgc
    19921 ggatacctcc ggaggctagg atttccatag aagtgtctgc ttcatttgct tcaagcaatg
    19981 aagagactgt cgattgcccc cagtcagcta aataactcag ttcccgcttg gagcgacggg
    20041 cgttttcgat ctgggtgaag cttgttccgc ttcgaccgct gatatcaact gtatgaactc
    20101 ctaaagcagc tagatcattg agcgtttctc ttgtcatgcc aaaaccgact tcttttacga
    20161 tgagggggac gtcaatggct gtttgagttt cttgaataag tactttccaa ttggtaaaat
    20221 cacggtctcc ctcaggcata accaactctt gaggtgcatt caaatgaatc tgtaaggcat
    20281 ctgcgtgaaa caatcggata gcttctttgg ctctttcgac agaagtccct gcaccgatgt
    20341 tggcaatgat tttcccgtga ggatattctt gtcgcataat cgtataagta tcggctaaag
    20401 aagggacttt caaggcagcg ctgactgaac cagtggcaat catcaaatct gcttctcttg
    20461 cgacgattgc aagatcttgg ttgatttttt ttgttttttc gcttccgcca gtcatcgcat
    20521 tgatataaaa tgggctgctt agtgtaaacc caactgcctg tgtacttaga tcgacatcag
    20581 aaactgctat ttgcggcaaa gggttatgga tgatccgtac gaagtcaaat tcgttttttt
    20641 gtttatcatg aaaggcttta gctagtgaaa catgttcgtc ttttcgattc atcggttttc
    20701 ctttctttga tcgctataga catgtaacgg cagtggagtg atttctgctt tttcccatgc
    20761 actcattaaa ggaagaatgc cagatttctg gtcaacaatt acgattccgc aatcgccccc
    20821 acctgcacca gaagattttg cggctccttc atactgttca gctaaattac acaatttgtt
    20881 caaagcaggc gtttcgatga cgaccccagt gattgcagat aaatcatgca gtaattgtcg
    20941 gttttttcga atcatctgtt ggatcaacgt tacattattt tctttaaacc ctttgatcat
    21001 ttcgttgaca cattctgtac tattttttaa gaaaagctga taagccacca ttttatcttc
    21061 tctcgaacgg tgaacttgat cgaccaaatc agaagtagag gcagggctac ccgtccaacc
    21121 aatcaataaa cgtaaatctt caggagcaat caatggctca atggatagac ctggccaatc
    21181 cagggcaagt aactcactga tagaatgctg agtttcttgt tcttggagcc aaggatggtc
    21241 gaaggttgag aaagcgatcc agccaccata acagctagca gcgatgtcgc cgcaggaacc
    21301 attatcttga actgctaaat tggctagtgc tgcaatcttg aaaatctcca actgagataa
    21361 attcaacgcg taaaaaacat tcaaggcttt gaccgttgca acggtcacgg caccgctaga
    21421 acccaatcca tatttccggc catttgaact gtctaactca cttgttactt tcagatcata
    21481 aaaggataaa agaatgtttt tttcttgtgc atacttttca gtcaagcgaa tagcagcaag
    21541 gatataatga aaaggatttt cccgaatatc taaaactaat tctccgttgc gtcttgtcca
    21601 acgtacaggc atcccactat attgtgcaga ttggatactt ccgacttttc gtgcagattc
    21661 tacagtgact gtcacgaatt gatcgactgc agcgataact gctggatggc ctgtttcaac
    21721 aactgcatat tctccggcaa tatagagttt gcctggtgca gatacttcaa tcataaaaat
    21781 ctccctttat tcaataatcg caattcctgg accagcatgt gcagtaatta cttgttcttt
    21841 gctaaataat ttggtaaatg tttcttgtac ctcagataaa tggctgtttt ccactaaaac
    21901 tttgacatta gggccagcat ccatcgtaaa gtagcatgga atgccttgct ttctcaattg
    21961 tcgcaccgca tccatcgctt ttaagctgtc aggagaccag taagtgaatg gaggctgggc
    22021 agctaaagtc gttccatgca tttttagtcc gtttctttcc atcgtttctc cgagaagttg
    22081 gaaatctttt gttttgattg cttgtttcaa ttgatataga tccccttcaa cggaatctaa
    22141 ccagccttga taaaaactgg atgtttcgac tgtccgacgc atcccatttc tgctggacac
    22201 ttcttttttc tggtcgttga tcaaaacgaa aaccattgct aaatcgtctt cgaaagaatc
    22261 ggaagggact ggcttagcgt aagaacttaa gtcgtcatgg cctttttccc attcgacgaa
    22321 accaccgaaa atacttcggc aagctgaacc agacccgcgt cgagcaaatc ttgaaagaga
    22381 gagatcatct aatccaagct taagagcagt attgcaagcg cctgctaaag cagctagccc
    22441 gctggcagag gaggctaatc cagctgcggt aggaacgaaa ttctgactga ttactgaagc
    22501 ttttagtgag aggccggctt ctttacgaaa aaggtcaaga aactgactga ctttttttgt
    22561 tgctttttcg tcttgcaagg tgccatctag ataaaattca tctaccatat agctatcaga
    22621 aaagatgact tctgtctctg tataaaatgc atccaatgtt aacgaaaggc tgttgttcat
    22681 tggaaggatg agttcttcat ttttcttacc ccaatatttg attagagcaa tgttggtata
    22741 tgcgcgtgct ttgcctttaa acataggtat gtactcctaa tccttgtatc caagtttcag
    22801 cagcacccgc atcttcaagt gcttgactga tttcttgggc ttttgttttt gtttgtgcta
    22861 atgcaatcat acagccaccg cgtccaccgc cagttaattt ggcacccaat gccccggtat
    22921 cttttgctgt ttggatcaat agattaagaa aatcattgct gattgtcagc ttttccagaa
    22981 gtgattgtga ttcatccatt gtctgtgcca acgtttctgg tgaattttca atgattgctt
    23041 gttttgcttg cttcgtcaag tatcctaatt tttggatcat ttgtccagtt tcatgaggct
    23101 gggtttcaaa gaggtgagcg acatctttga cggcttctct tgtttgtcct ttgattcctg
    23161 tatcagcgac aatcaaaaaa gcatcgatgt tcaaagaaaa gtagtcgaaa ggatgccctt
    23221 ttgtaaaata aatcggctgc aagctgctag tagcggctgc atcgattcca ctaggattac
    23281 catgggcgat tttttccgaa agctggacat tttctaatag tatttcacga gacaaaggaa
    23341 atgctaagta atcataaaaa gcacgagtga ctgctgtggc gactgcagcg cttgatccca
    23401 ttccacgttc agcaggaatc gtgctttcaa tggtcagttg caagtttttt tggatgtcat
    23461 gctgtttttt taagtgaatg aataattttt tgatattttt taatgcatga ggagcttcgg
    23521 ctaaatttcc tgaatagtag gaagaaacca gttgatcgtc cattgcatcc agctcttcaa
    23581 ggaatgcggt gacttttgtt gcatagaaag gaaaggcaat cgctggttct ccataaacaa
    23641 ccgcgtgctc gcccatcaat atgatctttc cgcttgattc tccttggcca tagtttgcca
    23701 taatctaact ccttctcacg taaaagtgat ttttaagcaa aaaataaagc gcccaaaacg
    23761 agttgcgctg cttcacgaat ttttttgtcc actaagggtc tcaaaaaaca ttcctccttt
    23821 attttactaa aaccatctca gaagtaaaac taagaatacg ttttctaaag aaaaaacact
    23881 tttttcttaa gaattttttg actttttttc ttctttgact agaaaaaaat acaattgaca
    23941 ctactagata ttgggggtat atttaaaaag gttggagtta aacacaatat ttagtgatga
    24001 agtgagctgg aggtaaatga gcgtgatcgt tttagcaggt acaattggtg ccggaaaatc
    24061 aagtttaaca gaaatgatgg ctgaacactt tgatagtcag gcattttatg aatcgattga
    24121 tgataatgaa gtcttgccct tattttatgc aaaccctgaa cagtatgctt ttttgctgca
    24181 aatttatttt ttgaataaac gttttgcaag catcaaacaa gcaatgaaag atgacaataa
    24241 tgtattggac cgttcgattt atgaagattc actgcttttc catctaaatg cagatctagg
    24301 aagagcaaca gaaacagaag ttcgtgtgta tgatgaactg ctggaaaaca tgatggaaga
    24361 acttccctat gctgcccata aaaaacatcc ggatttatta gttcatattc gtgtatcttt
    24421 tgatacgatg ttggagcgta tcgaaaaacg tggtcgttcg tatgaacaat tgtcctttga
    24481 tccaagtcta tacgattatt acaaagaatt gaacagacgt tacgatcaat ggtatgaaga
    24541 atacaaagaa agtccaaaaa tccaaatcga tggagaccgc tataattttg tggaagatcc
    24601 tcaagcaaaa gaagaagtct tgaaaatgat cgaagaaaaa ttagcagaga ttcgccaaac
    24661 caaagtagcc atttgattta cagttgctca aataaagggt aaacctttat ttgagcgact
    24721 tttttcttta tactggaaac aaatgtcagg aggatagagt atgcgggaaa aacactattt
    24781 taatcaagac cgtaattttt ggatcaatgt acagcaaaac gatgtgctag ctgttaaaaa
    24841 cttaaaagaa gaatatggca ttagtgacga gatgctgact tattctctag ataaaaatga
    24901 acgggcgcgc gtagagtacg attcttttga tgaagcgtta ctgcttgtat caaatgttcc
    24961 gcaccagcaa aaagtcgata atcattatga aactagtcct atcgctttta ttttaaaaga
    25021 agacggtttg tttacattta ccacgccgaa taccgagtac gtgattcgac taattcgatc
    25081 gcttttggaa cgaatgccag acatgagtgt atacagttta ttgtttcgta cattgttttt
    25141 gatttctgat tccttttttc ctttaatcga ggaagtgaat agtgaacgtc agcggcttaa
    25201 tctgaaatta agagaaaaaa caacgaacaa gaatctgctg caactttcag atttagagat
    25261 cggtttggtt tacttagtta caggaacaaa acaaaatgta gtacttttag aacaaatcaa
    25321 ggcattggct atttatcgca agttgtcaga aaaggaaaaa gaagagttgg acgatgcgct
    25381 gatcgaagca cgacaagcag ttgaaatgac gaacttagct tcgcagattt tagaccagct
    25441 ctctggcact tataataatt tgctgaataa caacttgaat gacacgatga aatttttgac
    25501 ggtctggtcg ctgatcttga ccgttccaac tattgtgaca ggattcttcg gaatgaatct
    25561 ccagttaccc tttacccatt ccgttttcgg atggggaatc gccttgatca tcagtttggt
    25621 cttatctatt tggatgctta ttgcactgtg gcggcggatt cgttgatcta gtctttgatc
    25681 tgcaaaccaa gggcttgggc gatcacaagt gcttgctctt ggttgacgat tagtcccgcc
    25741 aaagaatctt gagataaagc gattgctgtg aagaaattag tcgtaaagtc taatcccttc
    25801 agagaagttc gaaaccagtt cgaaccggtc aactgattgt cagacaaaaa aaggtttttc
    25861 caagtgacct cataaaattc gctgtcttct aattgacaat gatcgaagga gacagctttc
    25921 aagtttgtac tactaaagga agccatcgaa gcaatacaat cagtgaaagt acagtctcga
    25981 agataacttt cggcaaaatt cgtacctgtt aatttacatt gatgaaacac gacttggtgg
    26041 aaactgccac caatccactc cagattggaa aaatcacatt tttcaaaaat gacattactg
    26101 cattcaaaat gacgtaaaac tgttttttgc atcgtaagtt tttggatatg actctctctt
    26161 aataccatat ttttggcatc gagatagctt tggtcacccc cgttgatcag aagaccagag
    26221 aagatctcct catcttctaa ataaaactct ccctttgtaa gaagaggaag agaaggtgga
    26281 ataggttcga tttttttcat gtgggttcac ctcgtttttc tttagtatag caaagaagcg
    26341 ggaatcactg ctagaaagtc tgctaatcta atcattttca gaaaaggaag acaaactgta
    26401 tgaaaaacat tgacaaaaag attagttttt gctatactat gatggttgag aaataaaagc
    26461 agaagcaccc gcttctcgcc ttagttgata atgtcactgg gctagatagt tttcactggt
    26521 tctattaggt gaagtacgcg cgggttctgt aagagaactc gtttttttat tgccgtttat
    26581 tcggtaagct tttctttctc tcataatatt tggaggtgaa tgaccatagc aaaggatatg
    26641 atggttaacg acggcattcg tgcacgagag ttacgattga tcggttcaga tggtgagcaa
    26701 ttaggagtta agacaaaagc agaagcattg aatattgcag aacaagcaaa cttggatctt
    26761 gtgttagttg cccctggtgc gaaaccacca gttgcgcgaa tcatggacta cggtaaatac
    26821 cgtttcgaac aacaaaagaa agaccgcgaa gcgcgtaaaa aacaaaaagt gatcaatgta
    26881 aaagaagttc gcttaagtcc tacgatcgac ttgaatgact tcaatacaaa acttcgtaat
    26941 gcacgtaagt tcttggaaaa gggcgataaa gtgaaagctt ctatccgttt caaaggccgt
    27001 gccattaccc ataaagaaat tggtcaaaaa gtcttgaatc gcttagctga agaaactgcg
    27061 gatattgcaa cagtggaaca aaaagcgaaa atggacggac gcagcatgtt cttgacgctg
    27121 gcaccgaaaa acgacagtaa gtaatttgaa tgactaactg gctgttggac agccgatagt
    27181 cggataacga aagattttga ggaggaaata tagtcatgcc aaaacaaaaa acacaccgcg
    27241 gatcagcaaa acgtttcaaa cgtactggta aaggcggatt gaaacgcttc cgcgcgttta
    27301 caagtcaccg tttccacggc aaaacaaaaa aacaacgccg tcaattgcgt aaagcaagca
    27361 tggtttctag tggcgacttc aaacgtattc gccaacaact agcaaaaatg aaataagaag
    27421 tacaaataac agaatctgaa gatttatttt tattaggagg aattatacat ggcacgtgtt
    27481 aaaggtggaa cagtaactcg taaacgtcgt aaaagagtgc taaaattagc aaaaggttac
    27541 tacggttcaa aacacacttt atttaaaaca gcaaaagaac aagtaatgaa ttcatacaac
    27601 tacgcatatc gcgatcgtcg tcaaaagaaa cgcgacttcc gtaaattgtg gattgctcgt
    27661 atcaacgcag cagctcgtat gaatggcttg agctactcaa aattaatgca cggtttgaaa
    27721 gttgctgaaa tcgacatcaa ccgtaaaatg ttagctgact tggctgttaa cgatgcagca
    27781 gcatttactg cattggctga ccaagcaaaa gaagctttgg ctaaataagc taaacaatta
    27841 taaagaggaa agaagataac gaaaagttat gttctttcct ctttttttat ttacaatcct
    27901 ccatcaaatg aaacaggaca ataacgtata agtctaaaat agcataatta aggtttttta
    27961 attgaaaact gaacatttat ggtcaccctt taatgaaagc gctaactaca taagttagaa
    28021 tgagtttgac tgatatatta gtttgcttat cagaaaaaca aaatggacgg aggaataaca
    28081 tgagtgatat tattattggt attttgttag ttatctcatt ctttttcatg gtctggtact
    28141 gtgtaaaagg ctacaacttg atggtcggct ttgccatcat ggctactgta tggatgggat
    28201 tagcgctagt cggaaacacg ttttcaccaa acccggcgat ggaagggcaa ggagtaattg
    28261 atgtgttgac gcatatctat acgacggggc cagctgagta cgccaaatcg atcttggtaa
    28321 atgtcttctt tggtgccttt ttcgggcgtg ttctagtaga tagcggaatc gcagctaccc
    28381 tgattcgaaa ggtagttgaa cttggagggg acaagccaag aatcacgatg agcttactat
    28441 gtattgtaac cgctgttata ttcatgtcga tgacaggaat cggaccagtg atttctattg
    28501 cagttattgt gttgccaatc ttgatgtcat taggtatttc agtaccggtt gcactgttct
    28561 catttatggg ctcgattatg gctggaatct ttgctaatat cgtcaatttc aaacaatatc
    28621 aaacgattta tgctggtttc aatccagcgg ctgaaagcta tacttataat gactatttcc
    28681 aaattggtat gattgggatg attgtaagct tagttgtggt actaacggtc gctaatatat
    28741 caatgaacaa aaagaaacgc tatgcaatgg cagcaaacgt accagctgaa ggtggcgacg
    28801 caccaatgat ctcatggtta gccgtattat taccagtttt aggggtcgtt cttttagatc
    28861 ttccaatcat cttaggtttt attctagcag gtatctgggc attgctgttt actggaaaat
    28921 tgcgcggagg ttataaagaa atctgtcgtc agtttgcgaa attatttaca gatggcgcag
    28981 tagatgttgc gccaatggtt ggtttcttga tgaccttagc gatgttcaat aattcagctg
    29041 cgtatgcttc tccttatttt tcagctattt ttggagattg gataccgcaa tcaccattag
    29101 tcttggcaat cgtatttgct atattgacac cactaggatt tttccgcggt ccaatgaatc
    29161 tagtcggttc aggctcagct attttagctg tcgtattagc tgttaatcca acgatgtcgc
    29221 cagccttttt gttcccgtta tttgcaatca caacgattgc tcctcagcat ttagatatta
    29281 cacaatcttg ggttgcttgg ggattaggtt atacaaaagt aacttctcga gaatatatga
    29341 agaaatccat cccaactggt tggatcatcg gagcgatctt atgcctgatc acgttcttat
    29401 tgtacgggaa cgcctagtaa acaaaacgtc gataaaaacg attagatgaa tggaggaaaa
    29461 gaaaatgaaa cgttttgtgc gaatgggaat tgatgttggc ggtacacata cgaaagcagt
    29521 cgcaatcgat aatgcgacgc acgaaatcat cggaaaatct tcagtgaaaa caacccacga
    29581 tgatgtccgt ggagtagccg caggtgtcgt tcagtctttt caaaattgtt tgctggaaaa
    29641 taatatcagt ccagaagatg ttgtgtttgt tgcacattct accacacaag cgactaatgc
    29701 actgattgaa ggagacgtag caaaagtcgg cgtaatcggg atggcaaaag gtggattaga
    29761 aggtttttta gcaaaacgac aaactagatt gaacgatatc gatttaggga ataagaaaaa
    29821 aatagagatt gtcaatgcat tcttacctgt caaacactta aatgtagacc gtgtttcaga
    29881 aacgatttct tctttagaaa gagaaagagc ggaggtcttg gtttcttcta tggcatttgg
    29941 agtagataat ggggaaccag aacgagttgt ttatgaagca gcatcggtaa aatcgatccc
    30001 tacaacgatg gcttctgaca tcacaaaatt atatggattg acaagaagaa ctcggacagc
    30061 agcaatcaat gcatctattt taccaaaaat gctagatacg gcaacctcta cagaagattc
    30121 cgtacgagaa gcaggggtca atgtttcttt gatgattatg cgcggagacg gcggagtaat
    30181 ggaaatcaat gaaatgaaaa aacgacctgt tttaaccatg ctttccggtc cagcagcatc
    30241 agtcatgggc tctttgatgt acttgagagc gtctaatggt gtttattttg aagtaggcgg
    30301 aacaacaacc aatatcgggg tcattaaaaa tggacgtcca gcaatcgatt attcaattgt
    30361 aggtggccac ccaacatata tctcttcatt agatgttcgg gttctaggtg tggcaggcgg
    30421 ttcgatggta cgggctaatc agtcaggaat catcgatgtc ggtccacgtt ctgcacatat
    30481 tgccggtcta gattatgcag tatttacgga gacagaaaaa atcaaaggtc ctaaggttga
    30541 atttttctct cctaaagaag gagaccctgc tgattacgtc aaggtagtga tggaagacgg
    30601 agaagaagtg actattacaa atacatgtgc agccaatgtc cttggattag tgcaagaaga
    30661 acatttttct tatggaaatg tcccatctgc acgaaaagcc atccaagcat tagctgatta
    30721 ttgtcatacg acggttgaag acatcgcaga acagatcatg gaaaaatcct atgctaagat
    30781 tgaaccggtg attttagaat tggcagacaa gtatcacttg gagaaagatc agatttctct
    30841 tgtaggtgta gggggcggcg ctgcctcatt gattacttac tttagtaata aaatgggtgt
    30901 gaaatattcg attcctgaaa acgccgaagt gatttcttct attggtgtag cacttgcaat
    30961 ggtccgagat gttgtagaac gtatcatccc ttcgccaagt aaagaagata tacgatcgtt
    31021 gaaaaatgaa gcgatgaata aagcgattga atcaggcgct acaccagaat cgatcgaagt
    31081 ccacgtagaa atcgatccac agacttcaaa agtaacggct atcgctactg gttctacgga
    31141 agtgaaagca accgatctaa ccaaggagat cacaacagaa gaagcactag agcttgctgc
    31201 agaggatatg cgattaaata aaaatgaagt ttgcttattg gaaaatacac ctttctttta
    31261 tgtatgcggc gaacagaatc gttcaaaaaa tgctggaagt cttcggatca tcgatcaaaa
    31321 aggatttatc aaggtccaac gtggacatgc ttcatgtatg aaaacaactg cagccaatta
    31381 tatgacagct gtcgaacaac tttgggaaga tatggcagtt tatcaaactg aattgattgc
    31441 ccgaccagaa ttttatctct gcttaggagc aagagtctct gattttacag cgacggatct
    31501 ggaacaacta cagcttttga tggatctgga agtatctact atggaaccgg aggaagaagt
    31561 gatcgtcgtt gccggaaata tcaaacaaac ataagttaga agagttacta aacgaattac
    31621 aacagatccc cgaagaaatt tggggattct accagtttcg gcgagattta ttttggaaaa
    31681 agattccttt gtccaagcag aaaattctta tacaacaatc aatcgattgt ggtatcgaaa
    31741 cggcatgtag catcaaaaaa aagtaccctt ttgccgatgt aggagaaatt tgtgaacaaa
    31801 tggctgttcc aatcgtttcc tgtgaatcag agcaaataaa cgaacgaatc acttttgcaa
    31861 cttatgcaga agatgaaggg attcgattga tgacggagcc gttagaaaaa ttaaaatgtt
    31921 caggactagc ttcgatctct aaagaaacag cgcaagcttt gattattgga catgaactat
    31981 ttcaccacat cgaagcttct gttaagggaa tttatacgca aagcgaaaaa attgtgctgt
    32041 ggcggttgcc tttttataca catcaatcaa acattcgtgc attgagcgag atagcagcga
    32101 tgagtttttc aaaagagatg aatcaaagcg atttttcgcc ttatgtatta gaggcagtct
    32161 tactttggcc atataatgaa attcgtagcc aagggatctt agaagaaata aaaaaaatag
    32221 aaaaaagatg tacggagtat gatttagatt acaaataaag agactgtgac aaaagtcgtg
    32281 tcacagtcct ttttttccga ataaacgatg ttctcctgtc aactcctcgg tcacaagtca
    32341 aaatattcaa caaacatgag aagctgtttt ttgaaattgt tccttgtgac tcggagatga
    32401 acggcaggct cacgacctct tggaaaacgg tgttcaaaag cttgcatctg cggtaataag
    32461 tccagccaag ttaaaaacat aagaagctat ttttacttgg ctgttcttat tacttgatgc
    32521 agggcagctt tttcacaacc tctcaataaa cggtgtgcaa tcagctgacc ctcggtatta
    32581 gctcaaaaaa aacggcaaaa tatgaggaac tattttgccg tttctttctc taatactcag
    32641 gtcaaaacgc tgattccaaa acctcttatt tgatgggtct gtcctgaata tcttcaaaat
    32701 aatcggggta attatgaatc acgtattctt gctcctcgat aatcatatgt aaatgcatcg
    32761 tgcaaagaaa tccgacttca tcaaagttat gatcaatcat tgattgaagc attgtctgat
    32821 gttcatctaa tactcgaccc cagtctaatt ctgaaatcgt caaacgtaac catctgaaac
    32881 gcgtactata actttctagc caatcccaaa tttctcgttt tccagcaatt tcataacaag
    32941 ttcggtgaaa atcagtatcc aaatcaaaaa aagctttttt attttgagcg attactgcct
    33001 gatcggtttg ttctagtatt ttttctagtt tagcttgatc ttgcttggtc attttcgcac
    33061 tgcattcttg caggatcggt ttttccaact tttcccgcgt ataacgggct aatgaagcag
    33121 atcggatatc gatttttgaa acataagtgc cggactgtgg aatggttttt acaagctctt
    33181 gattgcgcag ttggatcagt gcttctcgaa tcggtgtacg cccgatagct agttgttctt
    33241 ctaacacttt atcggagatt ttacttccag gtgctaactc ggaataaatg atttgtttac
    33301 gtatagattc ataagcttgc tgttgcaaat tattgataac catttaacct tcctttctat
    33361 gcagatataa tctattataa cataaatcgc ttacaaatcg tgtgggcata aaaacacagt
    33421 ctgcaaaaaa agccctgata ggcatgccta ttagagcctc gaaaagaaaa atattgaatc
    33481 aatctggtcg tacctcttgg atcggtttga cacgaccgcc gattctttta gcaaaagctt
    33541 cggattcttt tttgtctttg aaaatgagca gattgtctaa gtttttttcc cgagtgcgac
    33601 agccagtttt atcgatgtaa ccgtgcatta ctttaacgac atacatgggg atcacctgct
    33661 tttttctttt cattatacga actactcgac acaatacaag aaaaatgctt agacggtctt
    33721 tattaagaat ttacatcatc caaagaagcg tacgcttgga ataactgatc taactccgga
    33781 ttgactttgc tcagcttcac taatcgttgc gaatcagaaa gtaaaaaaca gtgtttacta
    33841 ctgccagtag tttttaattt tttgtcttca gttcccacaa tttcgtagct aaaatccaac
    33901 cgtgtccccg tatacttttg gatagttgga tggatgctca ctttttctcc gtaatggatc
    33961 atttctttat agctgcaatt tacttccaga acggggatga tgatcccaga ttcttcgatt
    34021 tttttatagg gaaaccctag atgtaccaat aaggctaccc gagcctcttc aaaccagcgg
    34081 atataattgc tgtgatgaat aatccccatt tgatctgttt cataataaaa aggttctcgc
    34141 acataacctg taaatttctc catgactcat gcctcgtttt ccattgattt tttacaaaaa
    34201 taattcatcc atttcggaag tgcttcgctg atttgtgtcg gcaaaacaat atattgttct
    34261 tttgccagct ggtctccaat gaaactatga agataaatag ctgctgcaat cgtttggtca
    34321 ttttgaggaa actgggctaa aaatcctgtg atcatcccag ctaaagtgtc acccatgccg
    34381 cccgttgcca tagcagcaga acctaaagga ttggtgtaga agtgctggtg accataaatt
    34441 tttgtctcat gacttttcaa aacgagcaca gcaccgattt cctgttgctt ctttttgttt
    34501 tctttatccc gttgttcttg tatcggtaga gaactcaacc gttgccactc catttgatga
    34561 ggtgtaagta cagtttgttc gggatagggt agcaaaagat gattagcagc aagtaaactt
    34621 aaagcagagc catcaatcac cagccattgt tcttttgatt ggttttctag tacttgtttg
    34681 aaacgttcca aactcttaga agaagtaccc aatccgggac caatcaagag tacatctgct
    34741 tgctttatta cggaaagaaa ctggtcagta tccgtccaaa ctacaatcat tgcttctggc
    34801 agatgactgt gaagagcact ccgatttttt tcatcagtta ctacagtcgt gagacccgct
    34861 ccgctgtgta cggcagctaa agcactcata atgattgctc cgccatattg ttcattgccg
    34921 ccaatcaaaa cgatacgtcc aaatgttcct ttatgtgaaa tttttggacg aggtgtgatg
    34981 actcttttca ctagttgttc gtttatcagc atctaaaaac ctccttttac tcattatagc
    35041 gtatattttt tttagagaaa aacagataag aaaaggaatt gtcagatttt ttgaactatt
    35101 tttttctgta atttgtggta tcatgaacac gaagacttaa ataagtggag gattaacttt
    35161 atgacaattg attggcaaaa agaagtagaa gcacgtaaag aagacttgct tgaagacttg
    35221 aaaaatcttt tacgtgtcaa cagcgagcgc gatgactcaa aagtaacacc agatgcacca
    35281 tttggacctg gacctcgcga cgcattgaaa catatgttag cttatggcga acgtgatgga
    35341 tttaaagtaa aaaatgtcga taactatgca gggcatattg atcttggcga aggagacgaa
    35401 acgctaggga tatttggaca catggatgtc gttcctgcag gagatggctg ggacacagat
    35461 ccttatgaac cagtaatcaa ggatggaaag atctttgcac gcggatcaag cgatgacaaa
    35521 ggtccaagta tggcagctta ctatgcgatg aagatcatta aagaacttga cttgaaacta
    35581 tcaaaaaaag ttcgcttcgt agtcggcagc gatgaagaaa gcggctgggg cgatatggct
    35641 tattatttcg aacatgaaga agaaccagat tttggtttct caccagatgc tgaattccca
    35701 atcatcaatg gtgaaaaagg aaatgtttct ttagctttac gcttcaaagg cgacaatgca
    35761 ggagattatg tcttgaaatc ttttgtttcc ggacttcgcg aaaatatggt gccaggaaca
    35821 gcaactgcag cacttcaagt accaagtgca gatgcagcta tcgcgatgga agaagcattt
    35881 taccaattca tcgaagcaaa tccagtaagc ggaacgattg aagcggacaa tacgtatgta
    35941 aaaatcgaat tagttggtaa aggcgcacat ggtgcaagcc cacaatctgg tatcaatgca
    36001 ggttctttct tggcattgtt cttagataac tatgaattcc taggatctgc taaacaattt
    36061 atccatgtag cagctgctta tgtccatgaa gatttttatg gagaaaaact aggagtcgct
    36121 tatgaagatg aaaaaatggg caaattgaca atgaatgctg gactatttgc ttttgaagaa
    36181 aatggtacag aggaagcgaa cttcatcaat atgaatttcc gttttccaaa aggtgtaaca
    36241 gtagacggat tgcaaagtga catcgaacaa acagtggggc aagaaggcgc aacagttact
    36301 cgaggtgctc gcgtaatgga accacattac gtaccaatgg atgatccatt ggtggctaca
    36361 ttattgcaag tctatgaaga ccacactgga gaaaaaggct acgaacaaat catcggtggc
    36421 ggaacttacg gacgtttgtt gaaacgtggc gttgcttatg gagcgatgtt cccaggatat
    36481 actgatacaa tgcatcaagc aaacgaattt atgagtcttg atgacttgtt cagagcaaca
    36541 gcaatctatg cagatgccat ctatcgtttg gcgaaataat taattaaaaa gaggatcaag
    36601 aattgcaaat tatttttgca gtcttgatcc tttttttact ttctactgtg gtattcatga
    36661 taaacatgat atagattcaa ttcatattct ttcttagcgt tcgtgacgac tgtatccaaa
    36721 atcaaccctg ttactaatga aagaagtgct aaagtcatca atccagtact taaaatcgca
    36781 gaagggatgc gtgtaataaa gcttgtcagc atatattcgc gaatcacagg aatccccgtg
    36841 accaatccaa ataaaaagaa gaagatggtc caaatcccaa agaaaagcaa aggcttataa
    36901 tctttaaaca ttttaataat catcatgatc actttgaatc cgtcagagaa tgtattcaat
    36961 tttgattcgc tgccttcagg acgatcacga taatcgattg ggatttcaac gaacttgaat
    37021 tttttatcta aagcatggat cgtcaattcc gtttcgattt gaaaaccaga actcataata
    37081 ggaaaacttt tgacaaagat acgattgaat cctcgatagc cggtcatgac atcaagaatt
    37141 tttgttttat acattctagt aattgtattt ctaacaaggt tatttccaaa atcgtggaaa
    37201 ggtctcttat tctcatcaaa gtaggttcca tttgataggc gatcaccaat gaccatatct
    37261 gcttcaccag aacgtaattt ctcgagcaaa ccatgtacag cttctgcagg ataagtatca
    37321 tcgccatcta ccattaaata atagtcggca tcgatatcaa agaacatttg gcggatcaca
    37381 tttccttttc cttgacgagg ttcttttttt acgattgctc caccttgaac ggccaattcg
    37441 tatgttcgat cggtcgaatt attatcatag acataaatat ctgcttctgg taattctcgt
    37501 ttaaagtctg caatgactgt tgaaattgtt gcttcctcat tgtaacaagg aatcagaaca
    37561 gcaatttttt cttgttgttc catttttcac tctccttaac tatagaaaac aaaagctaaa
    37621 ttaaaaatac ctcataatca aaggaaatgc tagtttttcc cgctaaatat catttattta
    37681 aaaagaaaat actggaatat atgtttggaa agctctatct aaaaaaccac ccactacaaa
    37741 agaaggacat gatacctctc ttttgaatag tgggtgcgta ttcgttctct cacttttttg
    37801 acgtattaat ctgttgcacc tttttttctt gaaatttcta atacggttcc tgtatgagca
    37861 tctgctaaaa attcataata aacagtttcg ccatcttcta ttcgagaaag acctccccga
    37921 taagcactcg accgaacggc gaatttccta ataggttcct tttcaaaaga gatccaagaa
    37981 ccttcgattg caccttcttt tagaaaagat gacttgatat tttcaagtac ttggtctgct
    38041 gacaatactt ttttgcgatg ataccaaatc gttgaagcta ccccgctcaa taatcctaga
    38101 ccgattccaa ctgctaaccc gcctttaaaa tagcttagtt cgctttcttc atacatgtgg
    38161 acatacctcc ttcttctcac ttgttactcc ttgtgcttat tttaccacac tctcatattt
    38221 tctttgaaat aaataaccgc taccagaaaa aataagctat aataaatacg atcggtctgc
    38281 agaaaaaatc atattttatc cgcagatcaa tataagtctg agaggagttt agtctgaaat
    38341 ttagacgaat agcatatgga agaaaaaaca tttcaacgaa tcaaagagtt aacagaatta
    38401 caaggaacaa gcggttttga acaagatgtt cgcgcttata tggaaagtca tatggaaccg
    38461 ctggtagatg agttacagtt agatggtttg ggcggcattt ttggtctgcg ccatcatgaa
    38521 gaagcggatg caccccgtgt aatggtcgca gcacatatgg atgaagtcgg attcatgctg
    38581 acacaaatcc aagaaaacgg gctttttaaa gtcgtacctc taggcggctg gaatccttac
    38641 gttgtttctg ctcagcgttt tacacttaaa acctctactg gtaagaatta tccatgtatt
    38701 tcttcttccg ttcccccaca tttattgcga ggaacaagcg gacaaaaatc agtagaagta
    38761 accgatattt tgttcgatgc tgggtttgaa tcaagagaag aggcaatgag ttacggtgtc
    38821 cttccaggag acacaatcgt tccttacgct gaaacgatca aaacagccaa cggaaagaat
    38881 attatcagta aatcatggga caaccgctat ggctgtacaa tggtattaga agctttagaa
    38941 gcgttgcaaa atgaaacatt aggtcataca ttgattgcag gcgcgaatgt ccaggaagaa
    39001 gtaggcttgc gtggttctaa agcttctgtc aataaattca agccagatct gttctttgca
    39061 gtagactgct ctgcagcaga tgatacagtg acgaaaaatg gcacattcgg acatttagga
    39121 gaaggtacat tgatgcgtat ccaagatcca ggtttgatca tgcttcctcg tttgcgcgag
    39181 tacttgctgg atatcgctga aacaaacaat atcccttatc aatactttgt ttcaaaaggc
    39241 ggtacagatg caggtgctgc tcatacacaa aacgaaggaa tcccaagtac agtgattggc
    39301 gtagtgggac gttatatcca tacgcatcaa acgatgttca gtattcgcga ttttgaagct
    39361 gcacgagaaa tgttgattca aacactgaaa ggtttggata aatcaacagt aaatacgatc
    39421 gtatacggaa aataatgcga tagtgaaact ataaaatctg gcaaaagatt gctttttgcc
    39481 agattttttt tataaaaaga ggatcaatgt ttgttagttc gaaagaaagg tgtataattt
    39541 aattgaaaaa tacataaaca gaaagtagag gacaaagaat gattatccca aatagtttag
    39601 aagaattagc aggctacgta gaaactggaa agagtgtatt tttctttact gccggctggt
    39661 gcggagactg ccgttttatc aaaccgcaaa tgcctgaaat tgaaaatgac ttttctgatt
    39721 ggcaatttat tgaagtagac cgtgacaagt acatagatgt agccgctgaa tggaacattt
    39781 ttggtatccc aagttttgtc gtgatccaag acggaaaaga attaggtcgt ctagtgaata
    39841 aagatcgtaa aacaaaacag gaaatcgaat cgttcctaga aagtgtaaca aaaggctgac
    39901 atctgttagg aggggtcaag atgaatcaag aatataagaa tattttagta ggtatcgacg
    39961 gtagtgaaca agcacttgct gctttcaaaa aagcggtaga agtggctcgc cgtaataatg
    40021 ggaaagtata tgtagcaaac gtaatcgatc agcaatttta taactttatg ggctatgcac
    40081 ctattgatca aaatctagtc gatcagcaaa ctgaaacagc caaggaaatg atcgaagaat
    40141 gtaaagaata tgggaaatct gttgattaca caaatataga aggaattatt gcatatggtt
    40201 ctgttcgtga atccatggca gtcaaattgc caaaaaaata tgagatcgat ctaatcatgg
    40261 ttgggcagtc aggtctaaat gcagtcgaac gctttatgac aggaagtgta gccagctacg
    40321 tgatacgtca ttcaccttgt gatgtcttga tcgtttctga cgaagaagaa cagccaagta
    40381 attaagcaag tggaacttgt acgacctttt taatcaatta actaaaacag taaagggact
    40441 gtgacaaaag tcttgtcaca gtcccttgtt acgaataaat ggtgttccag aagaggcttc
    40501 ttcggaaata aagcatgaaa aaaatttcat gcagtgaatg caattttttg ttaagataaa
    40561 tctcagcaaa ggataatgga ggaaagaaca tgatttttgc ttataacaaa gcacacgtcg
    40621 gagacacctt gatggttatt atagccgacg ataaaggaac agaaaacact gttgaacgca
    40681 aatggaatgt tgctcggatc aaagatgaac aaggtcaagt tgttgcttgg aactttttcc
    40741 atatctccga tcatctaaca atcgaaggga acggccaagt aagcttaaca gatgaggaat
    40801 tagcgaaatt gaatcgattg atcaaagaag caggattcga agaacagctt gaagctgatc
    40861 atgaaccgaa gatcgttgtc gggtacgtaa aaacatgcgt cccacatcct gattctgacc
    40921 atttatcgat tactgagaca gaagtagaca atggtacagt attgcaaatc gtttgcggtg
    40981 cacccaatat cgaagctgga caaaaagttg tcgttgcaaa accaggagct atgatgccag
    41041 atggaatgat gatctggcca ggaactttgc gaggggtaga tagctatgga atgatctgtt
    41101 ccgctcgtga attgcatttg ccaaatgctc cagcaaaaaa aggaatctta gtattgcctg
    41161 aggatgccgt agtcggagaa gcatttccaa aataaagagg ttgtgacaag tctttgtcac
    41221 aacctctttt ttattaattt tcactatttt gattttgttt taatgccgat tgatcgatcg
    41281 tcaattcaac agtagttgtt ttttctttcg agccttcata gtaagtgatt ttgacagaat
    41341 ctcccacttt tttcttatac aaagcagatt gaagttctac accagaagaa acttctttgt
    41401 catcgatctt agtaatgacg tcgtattgtt tcaatccagc tttttcagcc ggtgtagctg
    41461 tttggacatt tgtcacgatg acaccgttag ttacagatga aggaatcttc aagatttgtt
    41521 cttgttgctg agtagaaacg gcagataagt cgaccattgt gatacctaga gctggacgtg
    41581 tcacttttcc gtctttttcc agttgattga tgatattcac tacatcgtta cttggaatag
    41641 caaatcccat tccttctacg cttacattag aagtagattc agaagtactt gcgatcttac
    41701 ttgagttgat accgataact tgtccttcga tattaactaa aggaccacca gaatttccag
    41761 ggttgattgc tgcatctgtt tggatcgcat tgatattgac tgtttcgttc gattcattcg
    41821 tacttgtcac ttgtcggttc aaggaagaaa tgattcctga agtgacagaa tttgcatatt
    41881 cagatcctag tggggaacca atcgcaatcg caggctcacc aaccttcaga gcacttgagt
    41941 caccaaaaga ggcaactgtt tcgactttgt ctgaattgat cttcaacacc gctaaatctg
    42001 aataagcatc tgttccaact aattcagctt ttaccttcgt accgtctttc atcaagactt
    42061 caagaccttg ctggccatct actacgtgat tattcgtgac tacataagca gtattcccgt
    42121 cttttttgta gatcacgccg cttccttcac tataagcttc caaattacta tcatctgagc
    42181 tgctactttc ttcttgctgc ccgaatagtc cgccaaaacc gcttgattgg ttttgacttt
    42241 gtaagttgat aacagaaacg acagcgtctt gaactttatc taccgctttg gtgatatcag
    42301 aatctacatt gactttgaca ttttccacaa ctgtttcacc ggcagaattc tgattgccgc
    42361 ttgtagcagt agaactattg ttgccagtgc ccatcgctgc ataaaaaatg ccagctgtca
    42421 gtagtcctcc aacgatccct ccaacaagcc ccagacctaa cttacgccag aggctattat
    42481 ttttactttt tttcatcttt ggtgtcacat tttttctatc catgtgttcc tcctccaatt
    42541 acattcattt atctactgtt agtatagtct tatttgttga ctttagtcta ctttgagact
    42601 gtgaactttt tatgaaaaac tttaggaaaa atcattgttt tcagatgaaa atatttgatt
    42661 ttctttttcc acagatactg tgtaaaacta cgcggatagg attggaaaaa taagggataa
    42721 aacatatcca cagcctgtgt ataaagtgga taaattctta taaaacgtat gtttgcctaa
    42781 tgaatgtgat gaaataaggt aaaataacac tgttttctgt ggataaacct gtggatgttg
    42841 tgcataactg ttggggaaat ctttttaaca aaaatagcca aaataaacat atagacgaaa
    42901 aaattgatta taataaacaa agtgagcatg aagaccaaag gagagaaaac atgaatatca
    42961 aaattatctc agtaggaaaa ttaaaagaaa aatacctgat ccaaggtatc aatgagtatg
    43021 tcaaacggct gaatgcctat gcaaaaatcg aattgatcga agttcctgac gaaaaagccc
    43081 cggagaattt aagcgaagcg caaatgagac aagtaaaaga aaaagaaggg gaacggatct
    43141 tagcgaaaat caaagaacaa gaatacgtct atgccttagc aattgaaggg aaaaatccta
    43201 caagtgaatc ttttgctaaa caaatcgacc aattaggtat ccaaggaaaa agccaccttg
    43261 tttttgttat cggcggctct ttaggactca gcgaagccgt catgaaaaga agcaatgctc
    43321 agatctcttt tggaaaaatg acgtatccac atcaattgat gcgtctgatc cttgtcgagc
    43381 aaatctaccg tgccttccgg atcaatgcgg gggctccata ccacaaataa tgatattttt
    43441 taa
//
LOCUS       DAZBSA010000012        40075 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000012 DAZBSA010000000
VERSION     DAZBSA010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.012"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            63..383
                     /locus_tag="ACHGMT_000599"
     CDS             63..383
                     /locus_tag="ACHGMT_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFN6834370.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(495..1352)
                     /locus_tag="ACHGMT_000600"
     CDS             complement(495..1352)
                     /locus_tag="ACHGMT_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFN6834371.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(1460..2764)
                     /locus_tag="ACHGMT_000601"
     CDS             complement(1460..2764)
                     /locus_tag="ACHGMT_000601"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN6834372.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(2761..3270)
                     /locus_tag="ACHGMT_000602"
     CDS             complement(2761..3270)
                     /locus_tag="ACHGMT_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834373.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(3445..5580)
                     /locus_tag="ACHGMT_000603"
     CDS             complement(3445..5580)
                     /locus_tag="ACHGMT_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFN6834374.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(5663..7318)
                     /locus_tag="ACHGMT_000604"
     CDS             complement(5663..7318)
                     /locus_tag="ACHGMT_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN6834375.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(7320..7634)
                     /locus_tag="ACHGMT_000605"
     CDS             complement(7320..7634)
                     /locus_tag="ACHGMT_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFN6834376.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            7804..8064
                     /locus_tag="ACHGMT_000606"
     CDS             7804..8064
                     /locus_tag="ACHGMT_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFN6834377.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            8078..8392
                     /locus_tag="ACHGMT_000607"
     CDS             8078..8392
                     /locus_tag="ACHGMT_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFN6834378.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(9122..9676)
                     /locus_tag="ACHGMT_000608"
     CDS             complement(9122..9676)
                     /locus_tag="ACHGMT_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFN6834379.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(9802..11731)
                     /locus_tag="ACHGMT_000609"
                     /pseudo
     CDS             complement(9802..11731)
                     /locus_tag="ACHGMT_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(12037..12753)
                     /locus_tag="ACHGMT_000610"
     CDS             complement(12037..12753)
                     /locus_tag="ACHGMT_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFN6834380.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(12754..14577)
                     /gene="glpO"
                     /locus_tag="ACHGMT_000611"
     CDS             complement(12754..14577)
                     /gene="glpO"
                     /locus_tag="ACHGMT_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFN6834381.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(14591..16087)
                     /gene="glpK"
                     /locus_tag="ACHGMT_000612"
     CDS             complement(14591..16087)
                     /gene="glpK"
                     /locus_tag="ACHGMT_000612"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFN6834382.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(16223..17683)
                     /locus_tag="ACHGMT_000613"
     CDS             complement(16223..17683)
                     /locus_tag="ACHGMT_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6834383.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(17673..18968)
                     /locus_tag="ACHGMT_000614"
     CDS             complement(17673..18968)
                     /locus_tag="ACHGMT_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN6834384.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(19300..19977)
                     /locus_tag="ACHGMT_000615"
     CDS             complement(19300..19977)
                     /locus_tag="ACHGMT_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFN6834385.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(20007..21098)
                     /locus_tag="ACHGMT_000616"
     CDS             complement(20007..21098)
                     /locus_tag="ACHGMT_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFN6834386.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(21199..21633)
                     /locus_tag="ACHGMT_000617"
     CDS             complement(21199..21633)
                     /locus_tag="ACHGMT_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFN6834387.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(22217..23674)
                     /locus_tag="ACHGMT_000618"
     CDS             complement(22217..23674)
                     /locus_tag="ACHGMT_000618"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN6834388.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(23671..25566)
                     /locus_tag="ACHGMT_000619"
     CDS             complement(23671..25566)
                     /locus_tag="ACHGMT_000619"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFN6834389.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(25624..27399)
                     /locus_tag="ACHGMT_000620"
     CDS             complement(25624..27399)
                     /locus_tag="ACHGMT_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFN6834390.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(27449..28945)
                     /locus_tag="ACHGMT_000621"
     CDS             complement(27449..28945)
                     /locus_tag="ACHGMT_000621"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN6834391.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(29139..30761)
                     /locus_tag="ACHGMT_000622"
     CDS             complement(29139..30761)
                     /locus_tag="ACHGMT_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFN6834392.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(31089..32402)
                     /locus_tag="ACHGMT_000623"
     CDS             complement(31089..32402)
                     /locus_tag="ACHGMT_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFN6834393.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(32647..32871)
                     /locus_tag="ACHGMT_000624"
     CDS             complement(32647..32871)
                     /locus_tag="ACHGMT_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834394.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(32891..33313)
                     /locus_tag="ACHGMT_000625"
     CDS             complement(32891..33313)
                     /locus_tag="ACHGMT_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN6834395.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(33455..34165)
                     /locus_tag="ACHGMT_000626"
     CDS             complement(33455..34165)
                     /locus_tag="ACHGMT_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFN6834396.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(34183..34680)
                     /locus_tag="ACHGMT_000627"
     CDS             complement(34183..34680)
                     /locus_tag="ACHGMT_000627"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6834397.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(34717..35169)
                     /locus_tag="ACHGMT_000628"
     CDS             complement(34717..35169)
                     /locus_tag="ACHGMT_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFN6834398.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(35304..35909)
                     /locus_tag="ACHGMT_000629"
     CDS             complement(35304..35909)
                     /locus_tag="ACHGMT_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFN6834399.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(36359..36979)
                     /locus_tag="ACHGMT_000630"
     CDS             complement(36359..36979)
                     /locus_tag="ACHGMT_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN6834400.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(36981..37850)
                     /locus_tag="ACHGMT_000631"
     CDS             complement(36981..37850)
                     /locus_tag="ACHGMT_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFN6834401.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(37873..38280)
                     /locus_tag="ACHGMT_000632"
     CDS             complement(37873..38280)
                     /locus_tag="ACHGMT_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFN6834402.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(38299..39588)
                     /locus_tag="ACHGMT_000633"
     CDS             complement(38299..39588)
                     /locus_tag="ACHGMT_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFN6834403.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            39646..>40075
                     /locus_tag="ACHGMT_000634"
     CDS             39646..>40075
                     /locus_tag="ACHGMT_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN6834404.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT        11423 a         8517 c         6763 g        13372 t
ORIGIN      
        1 gaaaatatgt gtagtgcttc ttgaagtttg ttatacttta attaaaagag aggagtgatc
       61 gaatgattac aacaacaacg aacagtgtag agggaaaagc cgtagtagct tataaaggga
      121 tcgtttttgg tgaggtcatt actgggatcc atgcatttaa agacttggag gcggggttgc
      181 gaaatatctt tggcggccgc tcaaaaagct atgaaaacga actaatgggt gcaagagaag
      241 aagcacttgc cgaaatggcc gaacgtgcaa aagcattagg agctgatgcg atcattggcg
      301 taaaaatgga ctatgaagta ttaggcagcg acaatggcat gctgatggtc acatgtagtg
      361 gaacagctgt ctccacgaga taagtagggg acatctgttt taataactaa agagagatgg
      421 gacatgtagt ggcactcaag aattagattt ttcagtctaa cttcgaggta ctctaccttc
      481 ccatcctctc ttttttattt ctttttgcgg atgattccgt aagctgctgc gactaacaca
      541 cccgcccctg ctaaggaata actgacagaa gctgtttttt catttgtttt tggcaaagtc
      601 tgacttgttg atttggatgg ctctgtcgtt tgctgaactg tttctgtcgt gcttccagag
      661 gtcactgatt tttctcctgc cgaatgcaaa atatctttta ttcgttggaa actagctgct
      721 aagtcagctt tttttgcttc aaaatcatta gcaactgttt gtgcttcgct gatctttccg
      781 tggattgctt gactggccgc tgtgatatcc gcttgagttt gacttagctt tccagaagct
      841 gtttgagcaa cctgatttaa ttgctgattg aattcttttg cttgttggat cttcccgtca
      901 agatctgttt ggacagcttc cgcttttgct ttcgcttcgg caagcttttt ctcaagttgt
      961 gcacggtcat ttcccacttt atttttaaga tcagctaaat cttgatcgat tgctgcttgg
     1021 tcttctttca atttgttttt tgcagcttct aatgcttctt cgagttcttt tcttttattt
     1081 tccaagtctt gcaaatgatc gatttggtca gaagaggtag aactatttat tgatgtatct
     1141 tctgaagatg aaatcgtttc tcctgaatta tctgtcggta cattggaagt catttctgtt
     1201 tccgaggcac cgttttcagc accagaagag cttatggttg ttgaaggttc tgtttgagca
     1261 ggttcagaag tttccgcaaa agctgtctgt cccaataaga aaatactgga aacagcaatt
     1321 ggccagatta acggcttcat tctttttttc atcacttttc ccatccttac tactttttaa
     1381 ctaatttctt attataagca aaaatgtttg gaagaaccat aaacacaaca ggctgtttgt
     1441 ctaaaaaacg gtgcatcttt tatttttttg tacgttccgg gcttttccat gtcccgcttc
     1501 cataacctaa aatcgttttg actgctggaa tgaaagtcgt cacgatcgtt atcgcgttca
     1561 ctatccagaa caaaagcata tatagtggtg cgaaaagaag gtatttcaat tttcttcgtc
     1621 gatcatctac aatcagcgaa gctagcaact ggaaaaatcc tgcgaccatt tcaaagcaga
     1681 tgaacacaaa actgaatgta aaagtaatat aaatctgttc gtagtttcct tgatatacat
     1741 aatgcccgat taatccagca aaaagaacta cggaaataca aaagaatatt gaccagacaa
     1801 tactcaatgt ttgatcaatg aagatgatcg ttcttccaat atgtttaaaa ggatggagca
     1861 tcactttttt gaaattcgtc agccagactt ctgttccacc ttttgcccaa cgttttctgt
     1921 gtcgatatag catcttcaac gtcacaggta cttccatgaa aaagataatc tcaggagcaa
     1981 aaacagataa ccagtcattt aactgatgat cccaagcaat actgatgtct tctgtcgctc
     2041 gatcttgtct gaatccgccc acatcgatca aggcttcttt tcgatacatc gtatttgctc
     2101 cactgtacgc ataaagacca ccgaaaacag ctgactgtgt tcgtttaatg atgcccacga
     2161 tacttgagaa ttcaaccgtt tgagattttg caatcaattt ggtacgattt tggacatcca
     2221 tatttgcagt gaccgcagca atatgtcttg caccagggcg aatgaaataa ttcacatacc
     2281 taatcagtgc atctggctcc ggtaccgtgt ctgcatcatt gcttaaaatc aatttccctt
     2341 ttgcaaaggc aagtccgata ttaaacgcgt gggcttttcc tttatttttt tcgatccgaa
     2401 ccacacgaag attagcgtat tttttcatca gtctagctaa tatttctggt gtttggtccg
     2461 ttgaaccatc gtctgtcact aatacctcat agttgtgata attcaatttc gtcatcaaat
     2521 actcgattgt atattcgatc acaatttctt cattatgggc tggcaccatg atcgtgatga
     2581 acggttcgac actcaaggga acatctaccc attctttttg cttatgtttg tataaaaaca
     2641 cataacacca gacaccaata aaccatacaa atccgcctaa aatcggataa gaaacgagaa
     2701 aaaaatacat aatagacaaa caagtatcta agaataaatt acctgtatgg gtgattgaga
     2761 tcatttcagt tccaccccat tttctttata caaatctgca atgaatgtcg tatctagatt
     2821 tttctcttcg gagacagaat aaaaacggac cgtttctctc tcttcttttg ttccaaaacg
     2881 ttccgtatac acttcttcaa gtaattcttg tctttttttc aatttttcat cttcatattc
     2941 aaaagattta tttacttttt tgtatagatt tcgattgttc cacctagtca atacaaatga
     3001 ggtaatcagg aaactgaaaa aaatcaatac aaaaaagccg attaagaaat acagcatttt
     3061 cttttctgat aaaaaagcgt acaaggaaaa atccttggct ctttctggtc tcgttaatgg
     3121 gaaaaagacc catataaaag gaagaacaac gatcatccat cctaaaagag ccactaacgt
     3181 ttgccaaatc tttaaccagt aattaccttt tgaaaaatac ttatctttca ccaaaattga
     3241 tgcaacttct tctcttgtta cttttttcat atcctcacct cacgtttttt tgttacgacg
     3301 taggagttta ccatacattt catttctttt cacataaagt gttcctaaat ttttcctaag
     3361 aaaaagaaca aaacatttct catcagcaaa tgaaaaatat tttgttcttt ttatcagttg
     3421 ttctttttat tttttatctc ttaattatgt tgctctttcc aatacgacaa tgcttttcat
     3481 tcctctagat atcgccgtgt acatttgtct gcgtcttctt tgctcatctc catcgttttc
     3541 aaatggatat aagattacat gatcaaattc cagtcctttt gctagactca aaggaatgat
     3601 ttggcagttt tctaatccat atgaattttt tttaatcgat tcttgtagcg tttgggtttc
     3661 ctcttctgtt tgggtgataa ctgccgcttt ttctcctttt agagaaacca aaatctcagc
     3721 tagtattttc aacaattctt cagaggatcg aaaagagcgg tattcgggtt cttttccttg
     3781 cggtcggatc gatacgatgt cgatcttctc ttgatccaca gcataggttt tgaaaagctc
     3841 tgtgattgct ccgctagaac ggtaactatt tctaagatca taccgatgga tcggcagatg
     3901 gtagtcgtcg aaacaacgca taatatcagc aaaagtagtg gaagaattaa agattgcctg
     3961 attctcgtca cctactaaag taaagtgagt ttttggaaat aattcgatca atagacctaa
     4021 ttgtgccagg gtataatctt gtacttcatc aatcaataga tagcgaaatg ctggtacatt
     4081 taccttttca acaagtaaat gacgaatagc taatataatg actgcttcat caacagtacg
     4141 tgtgggttgc caagcataag ctgagccata tcgtttttca taaattcttt caaacaactg
     4201 atgctcatcc acccatgcca tttcttcgat ttgtctggtt atttttcgat attttttttg
     4261 taaaagtttc cttgcataag ctacaatact ttgttccgaa tcatcagaaa tcaatttacc
     4321 gaataattcc aactgcctat cttctgtcaa cgagagtacc tgattctgca cagcaggact
     4381 ttttgcttgt ttcaaaagat gattcttcca ttcgctcatc agtgcctttt tcgttgcttg
     4441 aagcctatcg atcaatttag gatgagcagg aactttctca tatatttttt cgatcgtttt
     4501 tttagaaagg actgtttttc catttttagt aagtccacgg ataaaattcg gatgatctaa
     4561 aaacaaagga tctgattgtt ttagattatc gataaacttt ttgctacgca aacgttctgt
     4621 ctgctccgat acattttctc ccgtgatacg tttgaaataa gcctcttcac cttctatttc
     4681 ctctgctgaa agctgtgaaa ccagctgtat gatggttaga ttcagtggat ttctttcacc
     4741 taatgatggt aggacattcg aaatatagtc gataaaccga tgattaggtg atagaatcag
     4801 caagtcatcg gatgtcatcg tatctcgata taaatagagc agatacgcaa tacgctgcat
     4861 gatagcagag gttttaccac ttcctgctac gccatttacc aagatatttt gacttttcac
     4921 atcccgaatg attgtgtttt gttccgattg gatcgtagca gtgatagcct tcatttcatt
     4981 ggtctcatct tggcctaaga cttctaataa tacgtcatct tgaatagcta cagacgtatc
     5041 aaaaaacttg accaaacgat ctctttccaa aagaaattgt cgtctttctt taataaccgt
     5101 atcgatttta ttttgacgaa ccgtataaga agatggacca atcatgttat tgtaaaaaag
     5161 ttcggcaatt ggtgaacgcc aatcataaac aagattttca tctgcatcat ctgtgaaatt
     5221 tgctgtaccg atataaaagg attcttttgg ctcattatcc agaaagtcta caacgacttt
     5281 tccaaagtag ggacttttca ataaacgttc gatctttgct agctgatttt ctgcgttttt
     5341 ctgtttgata ttcatctgat cgatttctct atttttcatt tcaatcatcg aaaaagtatc
     5401 caggttatct aagtaactgt caaaattcaa tcgaagctcg ccagccattt ctttcaacga
     5461 ctggtttcct tctatttttg ccgtatccat cgattcccgc agttcgtttt ttttatcaat
     5521 tagcttttga tacacgttct tcagatatgc ttcttctaac tgtttttgtg tgttgaccat
     5581 acacattctc ctcttttttt aaagtggtgg aaccgattag tttacctctt ttaagagtcg
     5641 aatgcaagct ttttaacgag tatcatttgt taggatatat cagttcttca ggtcggacag
     5701 tttgatataa agaaaatgca ccatcaaggt tttttacgtg gtagccagac tgcttcaaga
     5761 ttctttccgc tagatagcta cgtaatccgc tatgacagct gacgatatat tcttgttggc
     5821 tgtctaattc atttaatcgg tcccgaagtt cattcaaagg gatatgtttg gcttccttga
     5881 atttgccctt ttccaattct tctgctgttc gcacatctaa taaaattttt ccttttgcta
     5941 gttctgtcgg caattcatgc cattgtacgg tttcactaag cccttctgca atgttcattg
     6001 ctgcatatcc tagcatgttt actggatctt tggctgagcc aaatggcggt gcatacgtca
     6061 attctaattc cggtaaatcg aagatcgtca aatgcccttt gattgctgtt gctaaaatat
     6121 cgatccgttt atccacacct tttgccccaa caccttgtgc accataaatc tcgccagtct
     6181 ctggatggaa gatcaacttc agtaaaatat ctgttgcttc tggataatag cttgcatgat
     6241 cttttcctga tacatgaatg acttcaaaag acagttcgtt ttctttggct gttctttcac
     6301 ttaaaccggt ggatgcggca gtcattccaa acgttcgtat aattgctgtt ccaataccgc
     6361 cttgatttct tcgagaaatt ccggaaatcg tgtccgcaac ttgtctacct tgacgattgg
     6421 ctggtgaagc aagagaaata agtgcatctt ttccagtgat ttgctgtttt acaacgatag
     6481 catcccccac cgcataaata tcaggaatat tcgtttgata acgttcgtct actaggatac
     6541 ccccgcgtat gcctaattcg attccagcgg tcactgctag ctggttatca ggacgaacac
     6601 caatggataa aataactaaa tcagcaggga tggtacgccc atcatccagt cgcaatcgtt
     6661 tcccgtggtc ttcaaatcca gcgacagcat gagaagtaat cacttctact ccttgatgag
     6721 acaattcttt ttctataaaa gctgccattt cttcatccag tgttggaaga atatgtggtg
     6781 cttgttcgat caccatgact tctaaacctc ttctttttag gttttctgcc atttccaatc
     6841 cgatgaatcc tgcaccaacg atcacggcac gctttggctg tttttctaat gcatgcatca
     6901 ctttatcgat atctggtaca tttctaatag aaaaaacatt gtcggcttct gttatccctg
     6961 gaatcgatgg aacaactggt tttgcaccag gagataaaac caatgcatcg tactgttcaa
     7021 tgtgtttttg accctcatgt acgacttcta ccgttttgtt ttcgggaaag atggctgtca
     7081 cttcatgatt tggacgcaca tctaaacaaa aacgagcgga taatgattct ggcgtttgga
     7141 caagaagatt ttcccgttca ctgatttctc ccgaaagata ataaggcaag ccgcaatttg
     7201 caaaagaaac atacggtcct ttttcaaaaa caacgatttc tgcatcttcc attaatcggc
     7261 gaagccgtgt agcagcagac attccacctg ctacacctcc gacaataacg attttcatct
     7321 tatttccctc ctactgttcg atcaaaccat tgattcatgc caccacgtac attcactaca
     7381 tcataaccag atttctttaa ttctttagct gcttgtttac tcctcatccc tgattgacaa
     7441 atcacataga cggtcttttc tttgtctcca tcatatctgt ttatactttg taatggaaca
     7501 tttttagctc cttttatatg cccgccccgg tattctgcag gtgttctaac atcaagtaac
     7561 gctggatttt ccaaaagctt ttcctttaat tctgggactg ttatcgattt tattgattga
     7621 aatagcaaat tcattttctg cacctcattt acatttttat aaatacctca tggggtatat
     7681 taaacaggag ctttattaat atgtcaacca gaatgactct tcattttttt cactactttt
     7741 tatatttgca taaaataccg ataaggggta ttatctaatt ataaaaatag aggagaatgt
     7801 gttatggaga ttgataataa aaatatcctg aaccgtttga aacgtacaga gggacaaatt
     7861 cgtggtatcc aaaaaatgat cgaagacgaa aaagaatgta tggatgtcat cactcaatta
     7921 agcgctgttc gttctagtat tgatcgcgta atgggcatga tcgttgcaga taatctgaaa
     7981 aattgctttg agaaacctga tagtaatcca gaagaacaag cggctaaact agaacaagcg
     8041 atcaagatga ttattaaaaa gtaaaggaat gatttcaatg aaagtcgttt tccatattga
     8101 tgaattagaa aaatggtcag aaacagggaa aaacgtaaaa aacctgataa aagcttctcc
     8161 agaaacgacg attgtcgtat ctgtaaatgg catcgcgatt accggttatc tagactctgc
     8221 caatgcagaa ttcttagatt tgcaaggagt cgtttttcac gcttgcgcca atgctatgcg
     8281 tgctaatcat atttcagaaa gttctttacc tgaacaagta atcgttgtac cagcaggtgt
     8341 ccttgattta gttgaactgc aatcccaagg ttatgcttat atcaagcctt agtcagtaga
     8401 ggttgcgaca gaagtgatca gcttcaagaa atttcggctt atttttcgaa gaagctactt
     8461 ctggaaaacc gtgaatcaaa ggttgtgaaa acgctgtcct gcatcaagta ataagaacag
     8521 ccaaacaaaa atagcttctc atatttttcg tttggttggg cttattaccg cagatgcaag
     8581 cttttgaaca ccgtttaagg aagaggttgt gaaatcagcg gtttgaccta agtattagag
     8641 aaaaaaacgg gaaaatagct cctcatattt tgccgttttt tgagctaata ccgaaggtca
     8701 gctgattgaa caccgtttaa ggaagagatt gtgaaaaagt cgcttagttt cgagtattaa
     8761 gaaaaatttt gtgaaaatgg atttctacat ttttgcataa atttggctta ataccgagaa
     8821 actggctttt gaataccgtt taaggaagag gttgtaaaat cagcggtttg acctgagtat
     8881 tagagaaaca aacggaaaaa tagctgctca tattttgccg ttttttgagc taataccgaa
     8941 ggtcagctga ttgaacaccg tttaccaaga ggttgtgagc ctaccattta actccgaatc
     9001 acaagaaata atttcaaaaa caacttctcc tgtttttggt tcggttggct tattatcgag
     9061 aagtagctac tgggagtcta tttatcaaaa aaaggaatgc gacataggtc acaccccttt
     9121 tttattttct ttgaaacatc tttcggtact cgacagcata caagacaaaa gattgtaaaa
     9181 gaccgtaagt gatgacggca aaaatgactc tcacccacag cgttggtaat tctgggaaaa
     9241 gcagcaaaag gatcatgcca acatagatga cagcagtcgc tactttgcca taccacttcg
     9301 ctccacttaa tttgatattt ttagatagcc cgattaatcc agctacaaac ataaagcctt
     9361 cttttatcaa gaatagacca aacaacagcc aaagccacgg tcgataccat gccatagaca
     9421 aaatcagaaa aagttgtgtc aatttatctg caaacggatc aagcaccttc cctaagtctg
     9481 ttacctgatt atatctccga gcaatgaccc cgtctagata atcagacaaa cctgagacaa
     9541 caatgaccgc tcctgctata taaaatgact gtctcagcac aaaataaaga taaatcggca
     9601 gcaaaaaaat acggaatatc gataaaagat tcggaatcgt ttgtatttct ttacgccagt
     9661 ctgtgcttaa tttcatttta tcgccctacc tcctcagcac tcttttcttt ttattttagc
     9721 ttacacgggg aataaaacca atgatttcac tgtttttatt tttctttaca gacactgata
     9781 ttatgagcta atttgagata atcacttgct gttttcatcg tttactcttc tttcttcctg
     9841 tcgtttgcgc atttctttta ctagtacttt atgagctttt tttgatcctc gctctaggtc
     9901 acgctgccgt ttacgtgttt tataatcgat aaacagactg tctaaatcgt aacctgtcgg
     9961 gtacagatct tctgctgaac gcaccagacg aacacgctga cgaggcacgt ggattatttt
    10021 tttgtctaaa tatacatcaa tcgtatcttc acctatatct tcataaacta acgcttcttt
    10081 ttggtattca ttgacaaata tccgatcacc ttttgcaaag agctgacgtt cttttttatt
    10141 ctccttccta tttgtaactg ctgtcttagg aagaaattct tttttctgtg tccgataggc
    10201 tttttccgcc aagtaacgct ctgcttgatg gatcaatttc atcgacatat ccattttatg
    10261 agcaatccac aaagcttggc tttctcctgt ttcttccaac ccgcaactga tatttaggac
    10321 gaagtgcttc ccgatcgaaa gccatagcag ccggcacaaa atcttcatga tctccggcga
    10381 atttcttgat ttctccataa tgggtcgtgg caatgatcaa tgccccttgc tcatacatag
    10441 attccatgat tgcgatcgct agtgcagccc cttcatttgg ctctgttcca ctgccaatct
    10501 catctaaaag taccaacgta ttccttttta tctttttcaa aatagcagcg atgttttgca
    10561 tatgaccaga aaatgtactc aacgcatttt ctaaattttg gtggtcaccg acatcgacga
    10621 aaatctcatc aaagacagca agctctgtcc cttcttttgc cggaacttgc ataccgaaca
    10681 tcgccatcaa agtcaataaa ccgactgtct tcaacacgac tgttttgcct ccggcatttg
    10741 ctccggtgat aatcaagcct cgatagtctt taccgatttc aacatccaat ggtaccgcat
    10801 gctcaggtaa aaatggatgt ctgccttgct tgataatgat atgttccgat ttattgattc
    10861 taggtgtgct tccattgatt tctcggctaa attttgcccg agcaaagatg atatctaaca
    10921 ccgtgatcgt ttcgatcaat aaatcgatca cttcttcgtt ttctgcgata gcaccagtca
    10981 atgccgccag gacttgatat tcttcactga tttcttcagc ttttaacaac tgatagtgtt
    11041 cattcaattt agaaatcaca gttggttcaa taaaaaccgt cgttctttta tttgattcat
    11101 cgataatcgt tccatctatt ttatttttat agctggcttt gataggaatc gtatagtatt
    11161 cgcctttttg aacaatcatt gcttcttgga tcatttcttt gtttttcgga tgacgcaaga
    11221 atttcagtaa ttttgactgg atttcttttt cgattagttg taattgtttg cgtactttac
    11281 gcaaattacg tgacgcgtcg tcactgactt tattattttt tattttctgg tcaatcaact
    11341 cttctacatt tatcaaatca ggaagatgtt tgctataagc aaacaacaag ggtgtctggt
    11401 attgattctt atcaaaaaac tttgtgatca tccgactgct tctaagaaaa tccgcatatt
    11461 cgatcaaatc ggttggctgc agaatcaacc cttttttgac ttgttctgtc agtgcatcga
    11521 tcctcggcaa ccccataaat ggaacatgct gactgctttc taagatcaag cgtgcttctc
    11581 ttgtttcttc ttgacgatcc aagacagttt gtaggtttgt cgagaccgtc atatcgctga
    11641 ttcgtttttt gctatagttt ccaattgctc ttgcttggat ttcctttttg atccgatcaa
    11701 attgtaattg actaatcatt tcgttgtcca tattctctcc ttttcttgaa gagatacctg
    11761 tcccaaaata aataagggcc tagagataaa taaaaggata gaacacagct taaaaaagtc
    11821 atgtcctatc cttattagaa tacaaagata ttgttttcct gacaggtacc gtttcataag
    11881 cacaccaaat aaactttgtt tattggctat tcgacttact gattagatac cagctacact
    11941 cacaaaacat accccattct tctaggtctt atttattttc cacttaacta tagcaagaca
    12001 cattttggtt gtcaaacttt agtatgtttt atagatttac accattaatg aatagacacc
    12061 taccgcgata attgctccga tcattggacc tgcgattggt acccatgaat atgcccaatc
    12121 tgaagttcct ttattagcta ttggcaatac cgcatgtgct aatcgaggac caaagtcccg
    12181 agcagggttg attgcatatc ctgttggccc tcctaatgaa agaccgatgg ctaagatcaa
    12241 tccacctaca gcgaatacgt tcgttccagg tgcaaattcg ttttgtccaa aagctagcaa
    12301 cccaagaact aatacaaatg taccaattaa ttctgtgatc acatttgctg gataattccg
    12361 tatagcagga cctgttgcaa acgttcctaa aatcgcgctt tcatctttcg ttgcattcca
    12421 atgtggcaaa taagaaagcc aaacaagaat ggcccctgca aaagctccta aaacttgcgc
    12481 aatgatgaaa ggaacgacaa gattccaact aattgcccct gtcatcgcca tagctagaga
    12541 gactgcagga ttcaaatgag ctgggcccat gaatccagaa atatagacag ccattgttac
    12601 agcgagtccc caaccaaatg cgatgacaat ccagccagaa gcctgtgctt tacttttgtt
    12661 taaattcaca gccgcacaca caccatctcc aagcaaaact aggatcagtg tcccaataaa
    12721 ctcccccatg atttgagtca tatcactgtt catttatttc tcccccttta gttcccgcag
    12781 atctgattct gcaatcaatg cttctaaacg tttagtctgt tgcgccttct cctcttctgt
    12841 ccattcaaaa tatccagcaa ttgcatcgat cacaggttgc ttgatctcat caagctgatc
    12901 tcgttcaaac aacagatgat ttgtacgacg aatgagataa tcgcctgggg ccaaaaccat
    12961 ttcttcttct aaaccataac gtaaccgagc tgactcagcg agactcaaac caggataagg
    13021 tgccatttct tttgctaatt caaagatacg tcgggcatta gtcccgtaaa aatcagcgat
    13081 ataagtagca tcttcttcct ctaaaccggc tgctactcct tcttttgcta attctgtcac
    13141 tgtttcttct aatttagttg gatcgaagtt tccacctgaa atctgatatt ttttagagtc
    13201 gatttctttc gtctctattc cgtattcttc ttttaataat tggcgtatca atcgcaaagc
    13261 tccttctgcc atcttacggt aatcggtgat tttcccacca gataaagtca gcaaaccatc
    13321 tggttctctt tctaaagaac tacctctgga aatcgtagaa ggtgcttttt catcacggct
    13381 gttttccaaa tggttcaaca cgtcttctac ttcagcacga gaaactttgt tttccttata
    13441 ttcacttact gtatcgacaa ctttattgaa gctcttgtct gaaatcgatc cattatctcc
    13501 accattgtaa tcagaaccag aattaccaat caacagtgga cgaagtcctg cccagcttgc
    13561 ttcgatatca ttcaatgtga tatttgcttc tggatagcga tgattgatca catccaatag
    13621 ataatctaca tcttcttgtg tgactttcgg atccgtaaaa tctccttggt aatccgtatc
    13681 tgtcgtacca aagtaagtct tgttttctct tggaatagca aaaaccatcc gcccatcttg
    13741 ctttcctgta tcgaagtatg tgggttgcgg tacaggcagt tttttcgcat ctacaactaa
    13801 atggatccct ttagttggac gcattttagg agagactgga cgcgtaaaat tcaagtttcg
    13861 tactttatct acccaaggac cactcgtatt gatcactaat tttgctttga tttcgatcac
    13921 ttcatctgtc agcaggtcac gagctttgac gccaacgatt tgatcgccat catataaaaa
    13981 gccaaccgct ttcattttac ttactagata agccccatct tcggcagcct ttttgatatt
    14041 atcaatcact aaacgggcat cattgttgcg gaaatctaga tagacacccg cgccttttag
    14101 cccttctttt ttcaaaaacg gttctctttc caatacttct tctggtgtca gcgtataatt
    14161 ctcatatttg gttcctgtca catttgccag tttgtcataa aggtccattg ctactttgac
    14221 cgagaacata ttgaaggttg ttgctccttc gccctcatag attggcaaaa gcattggatc
    14281 tggttttggg atgtgtgggg caatactttg tacgactgca cgttcaccaa ctgtgtcagc
    14341 tactacttcc acatcaaatg tcttcagata acgaataccg ccatgaacaa gtttggtcga
    14401 gcgagaggaa gtcccttctg caaaatcctg catttcgatc aatcctgttt tgattcctga
    14461 tgctgctgac tggactgcca caccggcacc agtgattccg ccaccgatga tcaacagatc
    14521 caattcttct tgctgcattt tttgaatgct atcttgtctt gtcttgtttg aaaacatatg
    14581 gatttcctcc ttattcaaat gcttgggtag cttttactgc ttttttccaa ccgttgtaaa
    14641 gtttttctcg gcgctcttcc tccatttgaa cttcaaatag cttgccagct tcataaaatt
    14701 cgcggatctc gtctgtatct ttccaataac cgacagctaa tcctgctaaa aatgctgccc
    14761 ctagcgccgt tgtttccaag ttttctgctc gttggatcgg tacattcaag atatctgttt
    14821 ggaattgcat cagatattca ttgtttgctg caccgccatc tactttcaat acaggaatct
    14881 tgattccagt gtcatcttgc atcgtatcga tgatatctct cacttgataa gcaatcgact
    14941 gtaaagtagc tttaatgata tcttcttttg tcgtaccacg tgtcaagcca aacatagccc
    15001 ctcgtgctgc ctgatcccaa taaggagcac ctagcccaac gaatgcagga acaacataaa
    15061 cctcatcttc gcttgtagag cgtttcgctg cgtcttctga atcgctagct ttttgcaaca
    15121 tttgaagtcc atctctcagc cattgaatcg aagaaccagc cacaaaaata cttccttcta
    15181 aagcatagta aacctttcca tttatgccgt aaccaattgt cgtcaataaa ttatttttgg
    15241 ataattgcgg tttttcaccg gtattcatga caataaatga acctgtacca taagtattct
    15301 tgaccatgcc tggttcgaat gccatttgtc cgaataatgc ggcttgctgg tctcctgcca
    15361 tccctgaaat tggtgcttcg cttccataaa aatgataacc ttgcgtataa ccataaactt
    15421 ctgagttaga agtgactttt ggtaacatca ctcgaggaat atttagtaaa tctaaaatct
    15481 cctgatccca atctagctga tggatattga acaacatcgt ccgactggca ttcgagtaat
    15541 cagtaacatg tgcttgtcca tcggtcagtt tccatactaa ccaagaatcg atcgttccaa
    15601 acatcaattc gcctttttca gctcgttctt gcgcgccttc tacatgatca agaatccagc
    15661 gtatcttcgt tgctgagaaa taggcatcga tcactaaccc tgttttttcg tggaaaaatt
    15721 cttgatagcc ttcatttttt aagttatctg caattccagc tgactgtctg gattgccaaa
    15781 caactgcatg atagattgga cgtcctgtat ctttttccca tattactgtt gtttcacgct
    15841 ggttcgttat gccaatccca gcaatctgat ttggtttgat ccctgattcg atgaacgctc
    15901 cggcaataac tgactgtaca gaattccaga tttcatttgc atcatgttcc acccaacttt
    15961 cttgcgggaa atattgcgtg aactcttttt gagaactgcc gatgtgtctt cctttcttgt
    16021 caaagataat tgctcgtgag ctagttgtac cttggtcaat cgccatgata tacgttgatt
    16081 ctgtcattgt gattccctcc attatatgca aacgctttct ttaactactc ttatcatact
    16141 actgttttaa aaaaagaaac tgtcaaaatt ttattctttt tttctttttt tgacagtttt
    16201 ttattatgaa gctatttttt aatcaattct tttctttttt cagcgtctct attcttcttt
    16261 tcaatcgaat aatgtcgtaa ggtgacgcaa attctgacag ataatattca cactgttttt
    16321 tgtttctctg gcaaaatgtg atcaccaaat catattcttt accttcttca taacttccaa
    16381 tctcaatacc gccgattccc ctcagttctc tgatcagata ttgttgaaaa gcaatacgat
    16441 aaatcggcag actttctaaa tcgattccaa tcactactgg ttttgcaata taaaaatcga
    16501 tcatcaaaag aacattggca tactggatca tcaggtaact aagctcacta tcttcagctc
    16561 ttttcctcgg caattgctgc accgctgttt cctgtaattt cgccagtaac tgtgttaagg
    16621 aatgcccgag cattttctgc tgctgttcca aaagccgatc acgctcatag acttcgatct
    16681 ttccaacaaa gaaaaaccat tcattgttga tctgggaaat ttggtagcct actgcctttt
    16741 cttctttgat tttcaaacga cgaggacgat aatgccataa catcgtttcc cgaatgtaag
    16801 tatctaaaac agcggttggt aactttttgc tccgtgtcaa atcatactga taaaatattt
    16861 cttcgtctac tatcgaaaaa gaaacaaaaa agctgtaaaa catcattgtt tcgtatacat
    16921 tcagttctgc tgctgttcga ctaagatgca tcacgatgat tggatcgatt gcttgataaa
    16981 gccggtccga ctgatagagt aatttctttt ctttcaaagt tgcgtacgta cttttttcat
    17041 tcaatagtct ttttttcgta atcccgagcc agcaagcaat ctgtgcttct gctgatgccg
    17101 taaacgttgt ttctaataca cggtccaatc ctagaatgaa cggtcttttt tctggtgtat
    17161 tttgcaaaaa aagcggtcgc tgatcttccg ggatatattg gaatagttca taataaaaat
    17221 atcgtatctg tagctcttct ccgtataatt gaccgttttt gatttgtaat ccaaattcct
    17281 ctaagagctg gttcaattct ttgatctttc gaaaaacaga agactcactg atattaaacg
    17341 ctgtcgctaa tcgaacgatg gatacttgct gatgttctaa taaatagacg agcatcttaa
    17401 acttcaagct ttcctgcaat aaaaacaata gaattttttc caaacttaca tcttcgtcca
    17461 acacaagcag cgcctgatac tcatttcgct gtacttctac ttttttttcc atcattcggc
    17521 caatcaattc aatatcttcc aaatattgat caaacgaggc ttttgacagt tggacatgct
    17581 ctctcaaacc attgatagag gctgttcccc cttgcagaac aaggtttcgc aaaatcagga
    17641 tctgtaactg ctcttttctt tctaataatg cttcaatctt cataggcagt attcgccacc
    17701 tttgaccaca ccatatctgg cacacggtat tctggctgga aaaatagctc atgttcttct
    17761 aactgttcaa acgtccatcc ttcttctact gcaattgtca gttggtcaag aaggtggaac
    17821 atctcttttt tgctttggag ctgtgctcca agaatctttt tttcagaatc aaatagaaat
    17881 ttgagtttaa ctttgctttg atttattgct gtaagcttgt agtctacgta tcctgtttca
    17941 attttttggg gataaaaaat gctctcttct tctgtcaaac ctacgcttcc taagaaatag
    18001 ccaaaccact gattgccgac tggacgatag gttccctgat cctttgtctg tgagccacta
    18061 attgtcttgc tgacatttcg tgctgtacgg acagcgttat ttactagaga aacatacatt
    18121 ttttcatttg ttggacgaaa agttacttgg atcgcatcgc caatcgcata aacgtctttt
    18181 tgagacgttt gcaagtagtc atctacccag atcgttccat catcattcaa tgttaattgt
    18241 tcttcccaca tctgattgtc gggacgagaa tgattagcca gcaaaacaag atcatagttc
    18301 tcctttcttt tttccgtctc tacaatagcg gtttcatttt cggtcaaaga cacaacttgt
    18361 tcttctaaaa aaagtgaaag cccttgattt ttcatttcct ttactaacgg ttctgtcatt
    18421 tcaggatcta gataacgaaa aagaattgac tttcggcttt cgtaaagatg gatttgtttt
    18481 tttcttgatg ccaacccttc agctatttcg attcctgctt gtcctgcacc aataattgca
    18541 attttctttg cattcatgat cttttgctga agagccgtta cgttttgtat ctttttaact
    18601 gggtgaataa gcgttgattc tgcagaaata tttcgaaact tttgatttga accagtcgct
    18661 agaatcaaac gatcaaaatc caattttttt cctcctgcta aaacgacttt tttttctttc
    18721 aatcctatta ctgtcttttc tgtgtaaaca tggattcgat agcggtcagt cagttcttct
    18781 ttcgtgatcc aagaaagttg tttttcactg gtaacctttc cttttaatat caaattgaac
    18841 ccgctgggaa tccatcctaa cttcgattgc ttttcaatca aatatatttc acttgttggc
    18901 atcaattggc gaagataaat agccgcatga atccctgcaa acgacccgcc gacaattaca
    18961 atgttcatct gcattcctct ttcttataag gtgttctact gctaacttct caatcagaaa
    19021 acacagtatt cataactcgc tttcaacctt atgatacata gcgatgcagc agaatcaacc
    19081 ctctccatca taccgtgttc taggtctgct tctcaataat gtactagtgg acctaattga
    19141 agagtatcat ttctgaagag atgtttcaaa aaatgtactc ttataaaaga aaaaaactcg
    19201 ccttttacgt acctctgtcc aaataggaaa cagaaaagaa cgaaaagacg agttttagcg
    19261 tgtgtaatgt aatagttaga atagacacat catttatttt taattcttta atgcttcaac
    19321 aactgcttcg cctaattgat gagcagattg aggattttgc cctgtaagaa gccgcccatc
    19381 aatttgaacc acttctgaaa aagctgctcc agactgatag tttgctcctt tttctttcaa
    19441 agcatcttct gctaagaatg gtacttcatt cgttgtacca tttgcttcct cttcttcatt
    19501 actgaaccca gtcactgtac gtccagctaa aatagatttt ccagactcat ctgtaactgg
    19561 caacaatcct acaacaccgt gacaaacagc tgagaccagc ccttcatttc gatagatttg
    19621 tgaagcgact ttcccaagat ctattgcttc tggaaaatcc cacattgttc cgtggccacc
    19681 agcataataa accgctgcat aatcatttgc atccacatca tcaggacgta aggtatttcc
    19741 caaggcccga tttctgaaat cttcatcttc ataataggcc caatcgattt catccatatt
    19801 caaaacactt tgcggttcta aaggtacata tccgccttta ggactgacaa aatctgcatc
    19861 gatcccattt tttactaata catcataaaa atgagtcagt tcacttaacc acaaacctgt
    19921 aggacgctcg atcttttcgt acttttctac atttgttaat acgatcaata ctttcatttt
    19981 cccatcctct ttctataaaa attgcgttat tgataacgtg ctttcaagaa attatataat
    20041 gggcttggca aataaagctc tttatatgct ttttcaaaag gtttcgttac aaattcatcc
    20101 tcacggtttt ccaataattt ctctgcccag tcaggatcga cgatcatttg ctgtccaatt
    20161 gctgcaagtt ctgcatattt caatacctgc tctacatctt cgcgattgcg gatcccaccg
    20221 acaccaacta aagggatctt tccttgaatc gtgttatgga ccacttcaag aatcgattta
    20281 tcgttgtatt tatcagaacg agctattcga tcatacatat ttaatgaaac atgcagataa
    20341 tctagttttg actcacgtaa tcgttcaaga agccagatcg tgtcttcaaa acggatacct
    20401 ggtgtttcat attcttctgg tgagaaacga taccctacga tgaatggttt tttagcatat
    20461 tgttcaaccg ctgagaaaac cgttgttaat aattcttcaa taaaacgata acgattttcc
    20521 aagctaccgc cccattgatc ctctcgtcta ttcgaatgcg gagagaaaaa ttgttggatc
    20581 aaatacgtat ttgctccatg caactcgatt ccatcaaatc ctgattcaat cgctctacgt
    20641 gtggctttgc caaatgcttg gattgtttca acgatttctt cttcactcat tgctcgtggt
    20701 gtttccgcat caggacgttt tgctgcaact gcacttgcag atacaggctg ttctccagat
    20761 aaagttgctc gacttgtcat tcgtccaccg tggaaaatct gaataatcgc ttttgcgcct
    20821 tctccttgga ttgctttcgc tagctcactt aatcgaggaa tcatttcatc atgagcgatg
    20881 ctgagttcac ctggccagcc ttttccgata tcttggacat ttgcagcacc agtaataaca
    20941 gcaccaagac ctttagaacg ttgtttataa taagtgatct catcttcggt gatcactcca
    21001 ttgaaaaagc tgagttgtgt cgtcataggc gacagcatca atcggttctt caattgaact
    21061 cctgaaggta aagtaattga ttgatcaaat tgtgacattg tcattcctcc cataattcgc
    21121 tctgattaac ctatcgagaa tcatctatca ttaaaccact cgattagttg tacatgcaac
    21181 taatttgctt aaaaaatttt attttttttg taacttatct gttgcatcat tcattgtctg
    21241 ggctaattct ttacttgctt catttcccaa aactgctttg tatgactgat gaagttcttc
    21301 ccagctacca tcgacttcct tagcaaattg gcgtccttgt tctgtaagat aaactaatga
    21361 caagcgacct tcgctaactg ttttgacgta tcctttatat tctaattttt caacaaaacg
    21421 agcaatcgtt gatggggcgg aatagagcat ttgtgcaata tctttttgtg gtattccatc
    21481 tttttctttt aatatcaata aaataaaacc ataagttggt gccagacctg tccttctaaa
    21541 cgcttcatca gctaatttat tcaacatgcg atctagcttt ttcacgctaa agaaaagaca
    21601 ttcatctaac gctttttcaa ttggcttttc catttcatcc cctccattgg cgtacatatc
    21661 atagtatgat ttttattttt tgtcaaaaaa cacggaataa tgtccgcctc ctctgtctag
    21721 atttgaaaaa aagaagtagc catcttcaaa gaatttcggc ttgttttcga caaaactcct
    21781 tctggaacac cgtgaatcag aggttgtgaa aaagtcgttt agttttgagt atgaagaaaa
    21841 attttgtgaa aatggctttc cacatttttg cataaatttg gcttaatacc gaaaaactgg
    21901 cttttgaaca ccgtttatat cagaggttgt gaaaaagccg tcctgcatca agtaataaga
    21961 acagccaaac aaaaatagtt cctcatattt tgtcgttttt tgagctaatg ccgatggtca
    22021 gctgattgta caccgtttat tgagaggtcg tgagcctgcc gttgaactcc gagtcacaag
    22081 gagcaatttc aaaaaacagc ttctcatgtt ttatgactat ggtgacttgt gatcgaggag
    22141 ttggcaggag aatactgttt attcgtaaag aaggggctgt gacaagactt ttgtcacagt
    22201 cccttatttc cttttattat aggatttcgc catttgattg gatgacttgt ctgtaccaat
    22261 caaatgattt tttctttaac cgtttctttg tgccattccc ttcgtcatct tgatcaacat
    22321 agatatatcc ataacgtttt gacatttgtg aagtagaagc gctgacgata tcaatgcagc
    22381 cccacgacgt ataaccaaat aaatctactc catctttgat agcttccttc atctgctcaa
    22441 tatgctgctt caaatagtca atgcggtaat cgtcttgaac ggttccatct tcttctactt
    22501 tgtctatagc tccgagtccg ttttcagcta tgaacagtgg tttttgatac ctatcatata
    22561 attgattcaa agcaattctc aaacccttag gatcaatctg ccagccccat tcacttgatg
    22621 gaagatacgg atttttaatt cctccagtta ttaagtttcc ttggacccct tcccaatttt
    22681 ctggatgagc actgactgca ctagacatgt agtagctaaa agaaacaaaa tcaacagggt
    22741 aggctgctaa gatttcttca tcacctactt ctatttttaa gttgacacct ttttcttcaa
    22801 acattcgttg cgtatacgct ggataatatc ctcttgcttg aacatcacta aagaacaaag
    22861 aagttctttg ctgctcaatt gttttttgaa tatcatccgg atcacaagaa tacggaacaa
    22921 gaagctgata gctgatcata caaccgactt gtgaaccttt gataatttca tgacaagctt
    22981 ttgtagctaa agcacttgct ataaactgat ggtgtgcacc ttggtatttc gcttgttcca
    23041 aatggggatg gttttcttca attactccta cgctaacaaa agggtgatgt tttacacagt
    23101 tgatctcatt gaaagtcatc caatatttta ctttattttt atagcgtttg aatactgttt
    23161 cagcgaaacg aacgtaaaac tgaataagct ttcgattagg ccatccacca tattctgtaa
    23221 ccaaatataa cggcatttca taatgggaaa gagaaacgac tggctcaatc ccatatttta
    23281 gacattcgtc aaacacttga tcataaaaaa ataatcccgc ttcattgggt gtttcttcat
    23341 caccatgagg aaaaattctt gtccatgcga tagacatccg atagcattta aaacccattt
    23401 cagcaaataa acggatgtct tctttaaagt gatgatagaa atcatttccg tgtcttttag
    23461 gataataata cttgctgttg ggatcctgcg ccgcttttac ttgctgatgc gtcatcatat
    23521 gccattgatc tgtccatttt tctttaggta aatcgctgtc gaatgtatac acatcagaca
    23581 cagataatcc tttttcatct acatcccatg caccttcaca ttgattggcc gctgtcgctc
    23641 cgccccataa aaatccttct ggaaattttt tcatatcgat ccccctaaac ttgctagttt
    23701 tcttgtttga ttatttttct tatgactagt aatgatattt ttgaatctac tactatttgt
    23761 aacaataacc attgtggtcg gatcatatcc ctctttgatt aattcttgca aatctacttc
    23821 catcaataaa tcgcctttac gtactttttt tccatcagat actgcacttt gaaagtattt
    23881 gccatttaaa ttcactgtat caataccgat atggatcagt atctctgctc cttcttcact
    23941 gactacccca attgcatgtt tcgttggaaa aactgttgaa acttccccgt ttacagaact
    24001 gtagattttt ccgtccgttg gaatgatcgc aaacccttct cctaaagatc cactagcaaa
    24061 tgttggatcg tttacttcag ataaatcaat tatttcacct gctgcaggag tacttaacac
    24121 tatatccttt gaggattctt cttcgatttc atccttgtag ccgaaaagaa aagttagcat
    24181 acaggaactg atcatcgcta agaaataact agcaactagc caaacaaatc cttcaccaat
    24241 ataaatcggg aaagacaaaa tacttcttgt agcaactgcc aaagcaccgg ctcctgcggc
    24301 accagctact ccaccaaata tagctccaca tatacaagca atgtaaaatg gtcgtttata
    24361 tttaagagtc acaccataaa cagcaggttc agtgattcca aataatgctg tgataaatgc
    24421 agataaagat aaacttttta ctttcttatt tttcgttttc aaaaataccc caagtactgc
    24481 tcctgcttgc gcagctacgg cagtcataca tgctggacct agcgtatcac gaccgtagaa
    24541 agaaagattg ttggtcatta ctggtacaag tccccaatgt aacccaaaaa tcactaacac
    24601 ttgccaaaat ccgccgataa cagcaccaga taaaacaggg ctaaagttat aaacagctaa
    24661 atatccagct gcaatcagct gacttgcata atctgcaatt ggacctacaa ctaataaaat
    24721 aattgggacc atcacgatga tacaaatgaa tggtgtcaat aaatttcgaa tactttcgtg
    24781 caagcttttg cgaatcttag gctcaatata agaaagaacc catacagcta aaattgctgg
    24841 aataaccgta cttgtatacc ttgctagaat cacgggcatc cctagaaatc gcaaggttat
    24901 gctatcacta tatgcactga caatcgtagg ataaacgaga gctgctgcaa taaccatagc
    24961 gatgaaacga tctgtattga acttttttgc agcagtatat gccaaaaata taggcaagaa
    25021 atagaaaaat gcatcagctg ctgcattcaa aatgatataa gttcctgatt cagtagttaa
    25081 taaattcaaa ctcgtacata aggcgagtac tcctttgatc aaaccacttc ctgccaagac
    25141 gcctaaagtc ggggcaaaaa cagcggcgat catgtctata aaagagttta aaatgccttt
    25201 tttctcagtt cctctctttc cactagatgc atcatcttct agtttcattt gtttggataa
    25261 tacgtcatac acttcattta ctgcatttcc tactacgact tgaaacattc ctccagcttc
    25321 gactacagtg atcacacctt ctagttgctc taccttctcc ttatctgcct tcttattatc
    25381 ttttagggta aagcgtaacc ttgttgcaca atggtaaaca ttgctgacat tttcttcgcc
    25441 tccgacaaat cggacaatat ctttcgctaa ttgtgaatag tccattttta cttccccctt
    25501 gttaattaat gccgtagcaa tccttcttac aagataagta tagttcttag aaaacggttt
    25561 ctccattcaa ttagtacccc ttctaatagc gaacgaaact gtacaatagt gtgtatggtt
    25621 tacttagccg agcaaatcac tcactgcttg aaagaaaaca agtatatcgt ttctcttata
    25681 gtcaaaggag acaaaaaaaa cacgcttgat cttcctttgc tgatgtataa gtggtatctc
    25741 aagctgatac tctgtgattt ttgtttctac ttggcttttg ataagacagc acaacaaagt
    25801 ctcggcattt aacggaagca taactaggtc agaactagga gagattagtt gctgaatgga
    25861 atctatcttt ttatggattt ttatttgatt ttcttctttg tagtaatgat caagcagttt
    25921 tgttttcttt ttttcttcaa ctatggtcag cttttcacgc aaaatctgat aaatctccca
    25981 agattcaaac tcatctagaa cactaggtag ataaatcaaa tcatcccact caaaaataag
    26041 ctcttgctca gtagtcaaaa ttgcatcata ttccgttatt ttgtttgatt gatttttgaa
    26101 aagatacact ggttctatcc ggcattcttt gatccacatg ttcatacttt gttgaatctg
    26161 tttttttagt gtattcaaaa tactaattgg ctggttacta acaatcagta tctccccttg
    26221 ttttcgaaaa ggattcaacg cttcggcaag atacaaaaca aaataagcgc tttcagctaa
    26281 attgggacgt aattgaaaat gtattgggaa gaaacgccga attaaataaa tttctaaagg
    26341 ataacgggag atgatctttt gtacaaaatg attcatggta ttatgtccgg catccatcct
    26401 cgttttcatt tgtttgatat gggttagcaa aaattctgtt tcatggaaca ataacgaaac
    26461 aggaagatct aaaaaacgaa taatcgtttg ctcaaatgct cttagattct tctctatttc
    26521 tttgtttttt tcatcattaa tcatcaaata atttaactct aatagtcgat tttcaattgc
    26581 ttgtttttct tgattggaaa aagaataggg tagttctgat gataactttt ttgtgagggt
    26641 ctctaccatc ggaataaata atgtatacct gtttgttttt tctaaaagtt cttctcttcg
    26701 aagaatggtc aaaacgagat accgagaaaa tcgtatgaag tcctctcctg aaacaatata
    26761 attatatgat ataagaacct cttttaatgt cttttggaca gtcggcatga tattggaaaa
    26821 aattgtactg atttgttgag gaaaccttgc gtgttggatc accgtttctg ttccaacaag
    26881 agataagaaa attctttttg aattttcttc agcaatgatt tttagtcctt ttgtcgacga
    26941 aacttccagt tctaatttag gattgcgctc gatccaacgc aaaatcgttt ttatttgtaa
    27001 tgatacggct gtttttgaaa gaaagaaaat gtctgctatc ttttgcatac tgacataccc
    27061 tttttgaaac aataagtacg tcacaatagt tgaagctcgg aaatccatgc tggaatagat
    27121 tttatgtttc gtcacctctt gtatgatttg ctgtttagtt tcagattcaa ttgattctaa
    27181 aaaataacct gaacgtttat ttgactggat aatgattttc ttctcgtaga tagcaagcat
    27241 atttttgatt tcagtttgga tcgttttctt gttggtacct aaagcttcag ctaattcttc
    27301 gcttgttatc caatgattct tatttcttaa tactacaaga atttggagtt gtcgtttatt
    27361 taagtccatt tgcttacacc tcctctttgt tataaacata caggaaaaac ctctcaaatg
    27421 acaatagtca cttgcagagg tttaaaaatc atttgaagaa agatctatat tcaaggtttt
    27481 taccattaga ctggataatt tgtttataat attcaaaact atcttttttc aatctcctaa
    27541 agcttccgct cccatcattt tgtctatcca cataaataaa gccatatcgc ttcctcattt
    27601 cccctgttcc tgccgatacg atatcgattg gtccccacca taagtaacca atgatttggc
    27661 aaccatcttt tattgcctca gcaatttcct gaagatgtat ttttagatac tcacaacgtt
    27721 ttggatcgtg gattcgattc aattcatctg gtgcttcatc tagtccaatg ccattttcaa
    27781 ctatgaataa tggtaattca taacggtctg tcaattcatt catgacataa cgaaacccta
    27841 gaggatcaat tggccaactc caaggttcgg gagatttttc cttcaaaaag ggattatcga
    27901 aaccatttgc tccgccagta tctacgcgca tttctgcttc ttttttatag acagcgctac
    27961 gataataact aaatgcaata taatctgccg tgttagcttg caagcactgt tcgtctccat
    28021 ctaacattac tggtgcacag tcttcttcct tccatgtcct gtaaatataa gctggataat
    28081 gccctcggca catgacatcg gagaaaaacc atgaattgcg tttaaattgc agtgaaccaa
    28141 atacattctc tggatcacag tcatatgcat aagtcgctaa tgcagaaaag ctacacataa
    28201 tacccatctt agcatttgga tcgattttct ttaataattg agtagcacgg gcgttcgcta
    28261 gaaacatatg atgacaggct tgataaatca ctttttttgt tagtgtagca atgggttcaa
    28321 gtttatctct agcagggaat gtaaccaatc cagcagctgc ataaggcata cgaaaagcat
    28381 tattgatctc attaaaggtc atccaatatt tcactttccc ttggtagcgt tcaaagacgg
    28441 tctgcgcata aaattcaaaa aagtcgatta attttcgatt ttgccacccc ccatactctc
    28501 ctaccaatgc taagggggtt tcataatgac taagagtaat tactggctcg atatcatatc
    28561 gatttaatgt attgattagt ctgtcataaa atacgagacc agcctcattt ggcttctgtt
    28621 cgtctcctct tggaaatatt cgtgcccatg agatgcttgt tcgaaaagct tttaaaccta
    28681 actccttcag taaacgaata tcctcttcaa atcgatgata aaaatcaatc gcttcatgag
    28741 aaagataatg gagtgattcg tctaaagcaa tctcccaaat ctttttttct tcattccact
    28801 gaatactagg atgctggttc atgatcccga taagcgtatc tgtcacatta ggtaatttgc
    28861 cgtcttccaa ccatgcacct tcacattgat tagcagctac tgctccgccc cataaaaaat
    28921 tttctggaaa tatttcctga tccataacta tctctcctcg ctatcaacaa tttcttactt
    28981 gaagtatagc tcgattcatc aaaacaagat attctaacta tttcggcaga agagaagaaa
    29041 aaaattgaat gctactcttc atttttaata tttatatata acaaatacat aagacgatcc
    29101 ccgaaagaaa acactcgtta accatctaag caaacgattt attttacttc caacacaaat
    29161 gcttcataag gtcgtaaatc aactttgccg tctggtaact gtttcagagg ataatttgac
    29221 acaataaccc ttaaagcttc acgtggaata gtgaacgtct gcttttcact ggacatatta
    29281 caaataacga gccattgctg gtcttcatat atgcgagaat aggcaaagac atgctcaggc
    29341 gtcttttcca ctaattcata gtctccccag acgatgatgt catgttttct acgcaaatcg
    29401 atcaatttct tgtacatata aaaaacagaa tcttgatctg ctaacgcttc ttctgcattg
    29461 atttctttat aatttggatt gactcgaagc catggaattc cagtagtaaa cccagcatgt
    29521 tcacgggcgt tccattgcat aggcgtccta gcattgtctc ttcctttcgc cttgattgat
    29581 tccaagattt cctcttttgt aaatccagaa gaaatccttt cgttatacat attgattgtc
    29641 tcaatatctt ttgcttccgc gatatcatcg attgggcaat tcgtcatgcc taactcttct
    29701 ccttgataga tataaggggt gcctttcatc atatggagca agatagcaaa catcttggca
    29761 ctttcttttc gatacacatc gtcatttccc caacgagaaa caattcgcgg aagatcgtga
    29821 ttattccaaa ataaactatt ccatccttca tcacctaaag atgtttgcca tttggataat
    29881 acttttttca gttcagagat actcaagggt ttcaaatccc atttgtcttt gccttcttgc
    29941 tgatctaatc ctatgtgttc gaattggaag accatggata attcatttct tttaggattg
    30001 gaataaagtt tagcgatttc ggtagttgct ccccatgttt cgccaacagt catcagattt
    30061 ttgtcaccaa aagttgcttg attcatttcc tgaagatact catgcagttt aggtccatta
    30121 cttgtgattt ctttatccgg ttctttgcca atcatatcga tcacgtccat acgaaagccc
    30181 ccaatacctt tatcgatcca gaaattcatc atttgataaa tctcttggcg aacctgttta
    30241 tttttccaat tcaaatcagg ttgacgcttg ctatacaagt gcagaaaata ttgccccgtt
    30301 ttttcatcca actgccaagc agaaccactg aatattgagc gcaatctatt aggctctttt
    30361 ccattgaccg gatcacgcca aatataatag tcccgataag gattttcttt atttttacgg
    30421 gcctcaatga accacggatg ttcatcagaa gtatgattca ccaccagatc catgatgata
    30481 cggatatttc ttttctttgc ttcttcaatc agttcttcca tttcttccat cgtaccgaat
    30541 tctgccatga ttgcttgata attactgata tcatacccat tgtcatcatt cggagactcg
    30601 tatactggag aaagccaaat tgcatcaata cctaaatacg ccagataatc caacctggaa
    30661 ataatccccc gcaaatctcc aataccatct tgatttgaat cttgaaaact tcttggatag
    30721 atttgataaa caactgaatg gtgccaccat ttttgttcca tgattttcct cctataaatg
    30781 gatgtttcta ttttttgctt aatcatttat tttagtctct ctcactaaaa attgtaacgc
    30841 tttcaaatag ctattgcacc ttgttatcgg taacagaata aaacaaaaaa acagctgagg
    30901 aaattccctc aactgttttt tgaaaggatt gatttatgca gggaagaatg ctgatttcac
    30961 aacttcttta taaacggtgt tcactcagct gtcccttggt attagctcaa aaaacggcaa
    31021 aatataagaa actatttttt cgtttttttc tctaatactc aggccagaac gctgatttca
    31081 caacctcttt atttcacatt caatatccac atgaatccca caaatacaaa gaacagacca
    31141 tacatgattg gagatacttc ttttcctctt ttagctgcta acatggtaat tggatagaag
    31201 ataaaaccta atgcaatccc atctgaaata ctataagtca aaggcatgcc gatcaagatc
    31261 aagaatgaag ggatcgcaat ctctaagttt ccccattcga tttgtcgcaa agattgtgcc
    31321 atcaatactc ctaccataat caatgctggt gcagttactt gtgaagtaac aactgatagt
    31381 aatggagaaa agaacaagcc taacaagaag aaaatccctg tagtaatagc tgtgatacct
    31441 gaacgtcctc caacggcgat accagctgat gattctacaa atgcaccaac aggagaagta
    31501 ccaaataagg aacccgcaag catagctgtt gaatcagaag ctaaagcttt ccctacacga
    31561 ggcatcttgt tgtctttcat gaaacctgct tggtttgcta atccaactaa agttcctgct
    31621 gtatcaaaga aagtcacaag caagaatgtc agcactacga cccacatttg aagagaattg
    31681 atgtccccta catgttttaa tgcaactaaa aatgttggtt tcaaacttgg tgctgcggca
    31741 acaatttcag aaggcattgg aatcaaacct gtgaccagac ctaaaatcgt tgtagctgcc
    31801 atcccgatga agatgccacc tggtacacgc cgaacaagca agatagcagt tacgactaat
    31861 ccaaaaatcg tcaaccaagt agaaccaacg ttgagtgatc ctaatcctac taatgtagat
    31921 tcattcgaaa cgatgatccc accttcgctc agaccaagga aagcgataaa tagaccgata
    31981 ccgccagaaa tcgcatattt caaatctgct gggatcgcat caatgatcat ttcccgcagc
    32041 ttgaagattg tgatcaaaat aaaaatcaaa gaagcaacga atacgccggc taatgctgtt
    32101 tcccatggaa tccccattcc taaacaaaca gaataagcaa aaaaagcatt gatcccaaga
    32161 gcaggtgccg ttccaattgg ataacgggct aataccccca ttaaaacaca gccgatcgca
    32221 ctggctaatg cagtcgcagt aaacactgct ccttcatcca ttccagaagc acctaataca
    32281 gttggattta caaataagat ataagccatt gagataaatg ttgtaaatcc tgccagtacc
    32341 tcacgtttta ccgtggtgtt caatttttct atttcgaagt aggaaagtat tttttccttc
    32401 atcttccatc cccctaaatc cgaacattat aaataaagtc cgttaaaatt tcgatttatt
    32461 aataaaacaa cgtgttttat catatactaa aacaaaacat aagtaaacaa aaaattcgga
    32521 ttttaagcaa aatccgaacg ttccatctga ttaaatccct ttatctcaaa gttcatgaac
    32581 attcactttg gattacaaga atttctttat tgtgttaata aagtttcttc attctagtct
    32641 caaaaatcaa tagtgtgatt tgattttacc atttcgtttc tgttcttctt tgatgaattc
    32701 ttttgtatcg acaggattca gattatttag tccgccaaaa cctgtatcga ccatttttcc
    32761 ccagcctttt gtcttctttt ttcctgattt gagattcttt gctcgaagct tctcactatg
    32821 ttttgctcgc cgatacttca aataaccttc taatcttccc attagatcca ctgtttttcc
    32881 tcctttttat tcaatcagac gactcagctt ttcctataac aaaataattg ccttcagggt
    32941 cagaaaaatt gaaagtcaag atgccattgt tatctgtgat ttctcctggt tcttccatgt
    33001 tctcgtagat agtttcaaat tcatccgtaa aaaatactaa agaaggcggt ggtcctaata
    33061 cctctggtga aaacttctgc acaaatactt ttgggaatac gcctaattct aattcttttg
    33121 agatgctgag tacaatactc ttaaattctt ctggcaattc gattgtttca gaaatttctg
    33181 caccaatttt ttctacaaag aatcggcaaa tcatctctac gtcattgaca taaagcatca
    33241 cacgtgtttt ttgtatcatc atttgaccct tctttctatt tgattttctt tcctgtctat
    33301 aaactgattc cattatatca cgaaagcgca aacaccttgt gtttgatact attcaagaaa
    33361 aataaagcaa tagtttctag gattgctttc ctccacaaac tttttttaca aaaaaaaaac
    33421 ggcaatgaat gccgctcttc ttatttcgat tcttttataa ttctgcttct gattcatctt
    33481 tggttagtgt gtgtctaaca ctagttacgc caaatgtttg atcgattttt tccttcaacg
    33541 cttcgattgc ctgtgcgcca cgtgccgctt gttcagcatt gatggattca atgaccagca
    33601 ccgctgcttg tgtttcttcc gtcagcatcg ttctttgggc ttcacgccag ttgagacagc
    33661 gacagatcgc tccttgttca tcatagtaga tgatttcttc cggaagtgcc ggagcatcgg
    33721 aatctgcacc taaaggcagg aatgattcgc caccttgggc ttttcctaaa tgcagatttc
    33781 ctgcgatctt ttcgatatct tctccgccgc aaggtacgcc aaaagacaag gaaatactat
    33841 tatagatatc tacaagcgga ttgattggga aaaattcccg ttcttgattt gcacgtttca
    33901 gtaaagcttc aatagaagaa cgtgcacctt ttttagtctt gaatgtggta aatgcctgac
    33961 gccactgagc aatgacttcg ttttggctga atgtttcttc tgtaaggtaa tttcttgctt
    34021 cttttgctgc ctcattcaat aattgatgat acgtttcttc actttctttt gttacgtggt
    34081 tatcgatacc atttacggat aagatttcga tggttgcttc aggaaatact tcccagaaat
    34141 tggtatccac tattactttt ttcatctgtt cacacctctt gattagaata attgcttgat
    34201 gaatagatag tctgtttggg gatcatctcc catataaaaa acatgttcgc tgtatttgat
    34261 aaatcctttt ttacgataaa aagcgagcgc aacatcgttg tgttcccaaa caccgagcca
    34321 aatggctgtt ttttgctttt cttttgcttt ttcaatagct gtatctagta aaaatgtacc
    34381 gataccttga tgtttaaatt tttttcgtac atagattcgc tcgatttcta aaaaatgatc
    34441 agccatttcc tcagattgac tcgttcctgt atttagtttc aggtaacccg caggctcttg
    34501 atcgatactc actaggaaaa aatcggtttc tttatctttt agctcttctg taagtttttc
    34561 tagtgcataa gcttgtgtca agtaatcagc taaatgctcc ggtgaattat cttttccaaa
    34621 tgtatctgta aatgtttcga tactaatttg ttgtagtaag tctacatctg aaggcgtcat
    34681 ttttcgaatt tcgtatttat tcacggcttc ttcctttcaa tagatccttt tattgccttt
    34741 tttgacaaaa ttccaatctt cgctaacatt cttgcatact ttatttaaaa gatattccaa
    34801 ttgttttgct tcttcgtcag aaagaccatt taatgcgact gcattggagt agttgttttc
    34861 tcgaatgatg aaaggataga tttctgctcc ttttttagtc ggatagagtt tcttgatttt
    34921 tttattttct ttatcttcca aacgttcgat catcccattg gattctaatt tcttgattgc
    34981 tcgggcagtc gttgtacgat ctactttgat catttctgct agtttttctt ggatgattcc
    35041 aggattttcg caaatacgta ctaaataaag atactgtcct cttgttaaat cgacttcttt
    35101 gaattcgata ttgctaatag aatctagcgc tcgggcaatc attccgatgt ctcgaaggat
    35161 ttcagccatt tcatcccacc tttattttgt ttgtaagact agttcacctc tttttgttgc
    35221 aaatgcaact attttcaata aaaaaatcaa gttctttttt aataactagt aaagtatatt
    35281 cttttcttct tttacgaaaa agctcaatca caatagatct ggtttttgtt ttctaaaata
    35341 agtaactgtt tcttcgttgt tcgcttttct gatagctttg gcaactcttc tatactaaaa
    35401 aatcccatgt tggaagtttc attattctca ataaacttgg cctcttcacc gtggatcgca
    35461 caagcaaaaa caagtttata atactgaaaa agctgtggaa tatctttttg tttattcgta
    35521 tcaaaaactg ctcgcaactg agtagtttct actgttaatc ctgtttcttc atagacttct
    35581 ttacgcacat tttcttcagg agacaatcct atttcagcaa atccaccagg aagtgaccat
    35641 tctttcgtcc gtttatcttc gattagtagg accttgccat cttttttgat ataggcacga
    35701 acatctactt ttggtgttgg atgtccttct ccaatcgttg tgagcgcttt tatttcttcg
    35761 aaagtttctt ttcctatttc actgatcagt tccaatgaaa tcgtccggat ctcctcaaaa
    35821 cgctctttat caaatacatc ttttccataa tatagaccag cttgagaaag tgcgagtagt
    35881 cttttgtatt gagccaactt cttgttcatt ctcttccctt cttttttagc aaatgtatga
    35941 ctatgtaatc aagtatagaa gactggttta attacggcaa ataaatataa tgaaatctgc
    36001 aataaataaa agaatgttaa aaaactcgag aacaaaagac attcgacttc tctggggctg
    36061 tgaataaatg gcgttcaaaa gcttgcatct gcggtaataa gccccaccaa acgaaaaata
    36121 tgacgagcta ttcttgtctg gctgttctta ttaattgagg caggacagct ttttcacaac
    36181 ctccccacta aacggtgttc tcctaccaac tcctcggtca caagtcacaa tattcatcaa
    36241 acatgagaag ctgttttttt gaaattgctc cttgtgactc ggagctgaac gacaggctca
    36301 cgacctctcg gtaaacagcg ttcaaaagcc aatttttcga tattaagcca tttctttgtt
    36361 acttattgtc attgatttct ttttctaatt tatccaatag tggagtgtaa tccacttgag
    36421 tatagtctgt tacatcaaat tcatatagtg ttcctaactg gaatttgtca tattttcggt
    36481 cggcgatgat ctttttgaat gcttctttcg taatatgatt ggtccctttc gttcgatctt
    36541 ccaatacgtc accatagtga taatgatcca tgatatagct caaatgacga tctgattcac
    36601 gatcacgttt gtaccgacgt tgccataaca cttcaaagtc tgctgttagg cgaatcgtga
    36661 tcacctgata tccgtatcta tctgctaatt cttggagtcg tgctttttgc ttatcactaa
    36721 aaggatactc ggacacaatc actttctttc ctgcttccat atacaaatca agtgctttgt
    36781 aatagaaagg ataaactttc gtcctttctt gttccgcccg ttcttccaaa ttgttaaacc
    36841 cgatggattc tgcatagtat tccttgattt catcgagcgt gatggcatac atatctggaa
    36901 atcgttcccg tagtttattc ataagatatg tttttcctgt accaggacta ccagccagaa
    36961 gaatgaaata ctttttcatc tcatttattt cctaagttaa ttggactttc caagttcata
    37021 aacagtccaa tcttatgctt gacaatttct tttactgctt catttgctgg aatcacatta
    37081 tggcgaagag acttgttgat ttctgtttca ctaaaggttt ctttagctac tttcgtccag
    37141 ttgactaaaa gctccgttcc aacattgaat ttgcggatcc cgttattgat caattcagga
    37201 taatcttctt ctttcacacc tgttcctcca tggataacta gggggacatc aactactgca
    37261 tcgatttctt tcagcaaggg aatgtttact tctgtttttg atttgaattg tccatgattc
    37321 gtaccgatag caatagctaa tgcatccacg cctgtagctt ctacaaattt cactgcgtct
    37381 tccggtcttg tatatacctt gtcattttca gcgacatgga ttccttcttc tgtaccgccg
    37441 attgttccaa gttctccttc tacggaaaca ccgtgcgcat gggcatattc tactactgct
    37501 tttgtcttca ggatattttc tttgaaaggc agatgggacc catcatacat gacagaagta
    37561 tatccgctgt cgattgcttc tttgatatca tcaaagtctc gggcgtggtc taagtgcaag
    37621 actgcatcaa ccatttcttc ttctgcaata gctgtcatta cattgactaa cacatgcatg
    37681 ccgatatatt tagcggtatc gacacttgtt tgaataatga tcggcgaacc catttctttt
    37741 gctgcgccta gcatagcagg aagcatttct aggttatgcg tattaaaagc tccaactgta
    37801 aaattcaact cttctgctgt ttttgttact tctttcaatg ttgtatacat cttttattgc
    37861 caccttatct ttttatttgt ttcctttgat cgttacttgt tttgcaattt gtcgcatctc
    37921 atccattttg cccatgtagt aatcgatccc ttgatcatca ccagcacgag cagcatctgc
    37981 acgcttctgg ttatagaggc tcatcagttt tttataccca tctccgatcg tctgtttatt
    38041 ttccaaactc ttttctaaat attggatggc ttgatcgttc acatacaatt cagaaaattt
    38101 aagtccaact tgttcataaa gtttcgctaa tttatgctta tcttgggtag tggaaatctc
    38161 ttgtttcaat tgtgtaatct ctgattgaat cttttcggtc gcttctggcg atagggcttt
    38221 ttcttctttt gccagaactt ttttcggttc tttttctttt ttcttaaaaa atccaaacat
    38281 cattttccct actttcaatt aaaatgcttt caaaactggg cttaagatcc aggcatataa
    38341 gagtgctgcg gctactgtat ctacatcggt tgctgtagca ttgttgaatc ccatcgaatt
    38401 aaagatcgtt actagcaaag cgggcaataa tgtaataaag aaaccatgag caataccgcc
    38461 aatgatcgct cctcggcgtc cgccaactgc gttgccaaat atccctgctg tccctccggc
    38521 aaagaaattg gttagcatac caggtaaaat catcgctaag ccgaacatgg gcaaagtaaa
    38581 catggcaata atcgtaccaa tcgttgtcgt aatgaaacct aaaatgacag catttgggct
    38641 ataaggaaag aaaactggac aatctaaggc agggatcgca tcaggaacta atttcatagc
    38701 tatccctcga aatgccggca cgatttcacc taacagtaga cgtacaccag ctagcaatac
    38761 atacacaccc actacgaatt ggatggcttg taaaaaggca aacatgatat agttttgatc
    38821 tcctgataat tcagaagcga atggttcgcc tgcaaataaa acagtgatga tatatagcgg
    38881 gaccatcacg accatgacag acaaataagt atcttgcaga aattcgaaat gtgtcggcaa
    38941 cttgatatct tcgatcgact ttttattttc gcctttctca ccaaaaagct tagcaacacc
    39001 ggcttcaaat aagtacccga tcgtacaaaa atgacctaaa gcaatatcat ttgatccagt
    39061 gattttacga atgatcggtt gcgctactgc cggcatagca atggcgaaac aagctccgat
    39121 aaaaccaccg accaagatca atacgatccc tcgcagaccg gcaaaatagc caaagactgt
    39181 cgtcatcgtt gccatccata aaattgcttg accagttaaa aaaatgtact tccattttgt
    39241 gaaacgagcc aaaataatat tgaatacaaa aatggctaag aacgtcagtg caatatctct
    39301 acctaaaccc aaatcattca ttgcttgacc attgatagat tcaatagaag ggatgatccc
    39361 ttgcatatga aatccttcag taaagatttt cccgaaataa gttaaacttc caacgatgat
    39421 gctcgaaccg gcactcaaca cttgaaatcc taggagtgtc tttaacgtgc cagagatgac
    39481 ttgtcctgct gatttcttct gcagtagtag acccaacata gcaatcaatg caatggtgat
    39541 cgatgcctga gtcaaaatat tttcgatgat aaaattgata acgcccatgt tttgatgtgc
    39601 accccaaaag ttagactaga aatctaatta aaaggggtgc tttttttgcg gaaatataca
    39661 tttgttttta agaaaaaagt agtttcagac tatttaaata acgaaggcgg ctacaaatat
    39721 cttgcacata aatatcaaat aaatcgtaca ctggttagac attgggtaag gatttataac
    39781 tatcatggtt gggaaggctt ggttggaggt ggcaaaagct acactacaaa atttaaactt
    39841 gatgttatag aatatatgga aacaaatggt ctttctatcc aagaaactgc taaaaaattt
    39901 aatatcggtt caaatagaac tctaagtaaa tggatagagc aatatgaaga aggcggtgct
    39961 tcttcacttg agagccaaaa aaggggcaga aaaattagta tgaattccaa gctaaacatt
    40021 cctaaaaaac ttaaagatga gtctcttgaa gaagaagtta ttcgtttaag agcag
//
LOCUS       DAZBSA010000013        39050 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000013 DAZBSA010000000
VERSION     DAZBSA010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.013"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(195..356)
                     /locus_tag="ACHGMT_000635"
     CDS             complement(195..356)
                     /locus_tag="ACHGMT_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834405.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(375..1397)
                     /locus_tag="ACHGMT_000636"
     CDS             complement(375..1397)
                     /locus_tag="ACHGMT_000636"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFN6834406.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAVNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            1525..1878
                     /locus_tag="ACHGMT_000637"
     CDS             1525..1878
                     /locus_tag="ACHGMT_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFN6834407.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            1890..3056
                     /locus_tag="ACHGMT_000638"
     CDS             1890..3056
                     /locus_tag="ACHGMT_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFN6834408.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVTLIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(3148..3768)
                     /locus_tag="ACHGMT_000639"
     CDS             complement(3148..3768)
                     /locus_tag="ACHGMT_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFN6834409.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(3960..4256)
                     /locus_tag="ACHGMT_000640"
     CDS             complement(3960..4256)
                     /locus_tag="ACHGMT_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFN6834410.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            4460..5110
                     /locus_tag="ACHGMT_000641"
     CDS             4460..5110
                     /locus_tag="ACHGMT_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFN6834411.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            5126..6853
                     /locus_tag="ACHGMT_000642"
     CDS             5126..6853
                     /locus_tag="ACHGMT_000642"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6834412.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            6850..8742
                     /locus_tag="ACHGMT_000643"
     CDS             6850..8742
                     /locus_tag="ACHGMT_000643"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6834413.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            9446..10315
                     /locus_tag="ACHGMT_000644"
     CDS             9446..10315
                     /locus_tag="ACHGMT_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN6834414.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            10524..11549
                     /locus_tag="ACHGMT_000645"
     CDS             10524..11549
                     /locus_tag="ACHGMT_000645"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFN6834415.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            11588..12538
                     /gene="manA"
                     /locus_tag="ACHGMT_000646"
     CDS             11588..12538
                     /gene="manA"
                     /locus_tag="ACHGMT_000646"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFN6834416.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            12632..14335
                     /locus_tag="ACHGMT_000647"
     CDS             12632..14335
                     /locus_tag="ACHGMT_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN6834417.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            14337..16370
                     /locus_tag="ACHGMT_000648"
     CDS             14337..16370
                     /locus_tag="ACHGMT_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFN6834418.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            16381..16821
                     /locus_tag="ACHGMT_000649"
     CDS             16381..16821
                     /locus_tag="ACHGMT_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN6834419.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(17021..18148)
                     /locus_tag="ACHGMT_000650"
     CDS             complement(17021..18148)
                     /locus_tag="ACHGMT_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6834420.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLLDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(18151..18828)
                     /locus_tag="ACHGMT_000651"
     CDS             complement(18151..18828)
                     /locus_tag="ACHGMT_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFN6834421.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            19092..20858
                     /locus_tag="ACHGMT_000652"
     CDS             19092..20858
                     /locus_tag="ACHGMT_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFN6834422.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            20873..21310
                     /locus_tag="ACHGMT_000653"
     CDS             20873..21310
                     /locus_tag="ACHGMT_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN6834423.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            21323..22474
                     /locus_tag="ACHGMT_000654"
     CDS             21323..22474
                     /locus_tag="ACHGMT_000654"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFN6834424.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(22536..23234)
                     /locus_tag="ACHGMT_000655"
     CDS             complement(22536..23234)
                     /locus_tag="ACHGMT_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFN6834425.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(23227..24579)
                     /locus_tag="ACHGMT_000656"
     CDS             complement(23227..24579)
                     /locus_tag="ACHGMT_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFN6834426.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(24802..26202)
                     /locus_tag="ACHGMT_000657"
     CDS             complement(24802..26202)
                     /locus_tag="ACHGMT_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFN6834427.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            26363..27100
                     /locus_tag="ACHGMT_000658"
     CDS             26363..27100
                     /locus_tag="ACHGMT_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFN6834428.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            27225..27704
                     /locus_tag="ACHGMT_000659"
     CDS             27225..27704
                     /locus_tag="ACHGMT_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFN6834429.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            27701..28468
                     /locus_tag="ACHGMT_000660"
     CDS             27701..28468
                     /locus_tag="ACHGMT_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFN6834430.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            28698..29267
                     /gene="lepB"
                     /locus_tag="ACHGMT_000661"
     CDS             28698..29267
                     /gene="lepB"
                     /locus_tag="ACHGMT_000661"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFN6834431.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(29371..29973)
                     /locus_tag="ACHGMT_000662"
     CDS             complement(29371..29973)
                     /locus_tag="ACHGMT_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFN6834432.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            30079..30309
                     /locus_tag="ACHGMT_000663"
     CDS             30079..30309
                     /locus_tag="ACHGMT_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834433.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            30300..30548
                     /locus_tag="ACHGMT_000664"
     CDS             30300..30548
                     /locus_tag="ACHGMT_000664"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6834434.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            30654..31238
                     /locus_tag="ACHGMT_000665"
     CDS             30654..31238
                     /locus_tag="ACHGMT_000665"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFN6834435.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            31241..32314
                     /gene="prfA"
                     /locus_tag="ACHGMT_000666"
     CDS             31241..32314
                     /gene="prfA"
                     /locus_tag="ACHGMT_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFN6834436.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            32307..33146
                     /gene="prmC"
                     /locus_tag="ACHGMT_000667"
     CDS             32307..33146
                     /gene="prmC"
                     /locus_tag="ACHGMT_000667"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFN6834437.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            33139..33318
                     /locus_tag="ACHGMT_000668"
     CDS             33139..33318
                     /locus_tag="ACHGMT_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834438.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            33340..34365
                     /locus_tag="ACHGMT_000669"
     CDS             33340..34365
                     /locus_tag="ACHGMT_000669"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFN6834439.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            34556..35800
                     /gene="glyA"
                     /locus_tag="ACHGMT_000670"
     CDS             34556..35800
                     /gene="glyA"
                     /locus_tag="ACHGMT_000670"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFN6834440.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            35953..36900
                     /locus_tag="ACHGMT_000671"
     CDS             35953..36900
                     /locus_tag="ACHGMT_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFN6834441.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            37034..37663
                     /gene="upp"
                     /locus_tag="ACHGMT_000672"
     CDS             37034..37663
                     /gene="upp"
                     /locus_tag="ACHGMT_000672"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFN6834442.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(37802..38044)
                     /locus_tag="ACHGMT_000673"
     CDS             complement(37802..38044)
                     /locus_tag="ACHGMT_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834443.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            38165..38237
                     /locus_tag="ACHGMT_000674"
     tRNA            38165..38237
                     /locus_tag="ACHGMT_000674"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38198..38200,aa:Thr,seq:cgt)
     gene            complement(38356..38994)
                     /locus_tag="ACHGMT_000675"
     CDS             complement(38356..38994)
                     /locus_tag="ACHGMT_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFN6834444.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT        13004 a         6959 c         7938 g        11149 t
ORIGIN      
        1 tagagtgatc aaaatccttt atgataatga catacttata accaacaaca accttgtact
       61 tactcaaaat cgagtaaata ctccttttcc gtcgagtcta accatctcgg cggtttttta
      121 tttgtagaaa agaacatcag gatagacgga aaagtcttgt cctgatgttc ttttcagata
      181 gtatggaaag ctgattagtt ttctacattc tgtacatatt gttcaaagta gttctgtaaa
      241 tcggctttga gtttctgata ttcctcttcg ctatactctt tatcttcgat tttcccatca
      301 aagaaaggaa tatttagttc ttcccgtagt ctgtctaata cgtcttgctg attcatttca
      361 ttctcctttt cttactaacg atcaaaataa acacagacac attctggtac atagacatct
      421 tttttcagta attccagtaa tcctgtttca ggatgacggc gatataatgt cagattatcg
      481 gtgttctgat tggcacacac gacaaaatta ttgcttggat ctagtgcgaa atcgcgggga
      541 aaatcccctt ctgttgagat gatttgcttg ttttgaatgt gggaaccatc ttcaaaaact
      601 gcgaatacag caatcgagtt atgtcctcgg ttagaagcat aaaggaactt gccatcattt
      661 gaaatgcgga tagctgcccc tccattttca ccgtcgaatt cttctggcaa ggtcgatatt
      721 ttctgtaatt cttcaaaagc acctgtttct ttattataag aaagcacgcg gactgtgctg
      781 tctaattcac caaataaata agcgaattgt ccatttggat gaaagacaag atggcgaggg
      841 cctgttcctg gctctgcagt gaaggtagtt acaagattca atttgccgtt ttcggacaca
      901 tcataagtat acacacgatc tgtaccaaga tcacatacga ctaatcgttg gtcaggagtc
      961 agatccgtat agtggacatg cgcatgatct tggtttttat gaggacctgt cgcttcttga
     1021 tggaatattg aatctgctgc tttcagttcg ccatctccta atatatggta gacatttact
     1081 tcacctttat gatagttcgc cccgtatagt aattgacgtg cttcatcaac cgctacataa
     1141 cacaaaggag cgccagactc tgttaccgca ttcaaaaaat gaaaatcctt atcgtacgca
     1201 ccggcaccac ctaatccatc cacactagta actgtgtaaa gattgacagc tttacttttt
     1261 gctacatagg tagggctatt ttcttcaaca gctaatgaaa ggttggctaa ttcttctttt
     1321 tctgtatcaa ggtcgatcgt atagattcct ttactttcgc gacgcgtata ggttcctaaa
     1381 attatctttt cgatcatatg agctgattcc tcctaattgt aatgaaatag ttttcgtttt
     1441 tattctatca cataattatt ccacacggct aaagaatgag aatgtgttat actgtttttg
     1501 aaagcaatta aaggagagag tgcaatgatt caggcaaaga ttacagaaat tgggacagaa
     1561 gcaataaaca aggaagaacc catgctgatt ttgttcgata aaacagcaac agctgcctta
     1621 cgaaaatatt cggtgattca agaagtatca aaagacgaac ctttttcttt gaaagcaggc
     1681 ggaacaattg cttttgatga tcaggtatat acgatcgatt atgtcggtcc gatggccaat
     1741 gggaatttaa agacagtagc tcacgtatca ttgatttttg atgcttgtcc agaagaagga
     1801 caaattgcaa atggaatcta tcttcatccg catagactgc ctgctattgg tttgggaagc
     1861 caaatttttt ataactaaca ggaggaatta tggaaggaaa aaataagaag aacagaccgg
     1921 tatcgtggtt ttggcgctgg tttttaaata atcaagtagt cacagcctta cttgttgtat
     1981 tgttaatatt gctgatcgtc tttttattta caaaagtttc atatttgttt gcaccaatct
     2041 ggcaattttt agcaatcgta ggtctgccta tcattttagc aggaatcttg tattacttga
     2101 tgaatccggt agttgattac tttgaaaagc gaaaagtacc aaggctttac agtatcattg
     2161 ggctgtttgt tttggttgtt acactaatcg catgggggag cgtagtgatc atccctaaga
     2221 ttcaggaaca aacagtgagc ttcatcagta acttccctca atatgtagat acgattgata
     2281 ataaattaac cgagattttg cgggacccgc tgttcaacca atttcgcgaa cagttggaaa
     2341 cagcaggtga taagttcatg aattcggcag gcgatatgat ccaagatata tctaagagta
     2401 cagttcagag tcttggaagt tttgtcggcg cggtagcgac tattttagtg gctctgctga
     2461 cgatgccgtt tatcttattt tatctattga aagacggtaa acaattagca ccttattttg
     2521 tcaaattttt accgacacgc atgcaaaaac cgacgttgaa tgtgctgagt gaaatgaata
     2581 gtcaagtttc ttcttatata agaggacaac taactgtggc ttttgctgtg gcaatcatgt
     2641 ttatgattgg ttttgcggtc attggtttag agtatgctgt cacgttgggg attattgcgg
     2701 gttttttaaa cctgatccct tacttaggat catttttagc gatgattccg gctatctttt
     2761 tagccattgt agcaggtcca ttcatgatca tcaaagtact gatcgttttt gcagtagagc
     2821 agacaataga aggacgcttg atctctccat tagtactagg gaatcagtta gcaatccatc
     2881 cagtaacgat tttattagtt ctgctgactt ctgggaaatt gttcggaatc atcggtgtga
     2941 tcttggggat tcctgtttat gcagcagcta aagtagtgat tactcatgtt tttgaatggt
     3001 acacaacaat ctcttctctt tatgaagaaa atccgttgaa aaagcagaaa gaataacaaa
     3061 aagagacaga tgggaaaata tccaaggcac aaatgctgct ttgaagattt tttcctctgt
     3121 ctcttttgta tgcatttatc tcaattttta aaatgattta cctaaagcag cagctttatc
     3181 taatgcattt tgcatcaact catctgcacg gtcagggtca taatcgattc cttcgatgaa
     3241 tagctgatct acttgatcga tgcctacaaa gtttaaaata ccttttacat attgagaggc
     3301 aaagtcttgt ccgttgtata caccgccatt agattggatg tgcagtgctt ttttacctgt
     3361 cactaatcct tctggcccgt tttctgtata gcggaacgtt tttccagcta cattgacggt
     3421 atcgaaccaa gcttttaatt ttgttgggat atttagattc cataaggcgt ttgcgataac
     3481 gattttatca gcagcaagga actgatcagt caattcattg aaacgagcaa ttttttgttg
     3541 ttgactttct gataaggaag tgaattctgc accagcacgt aatgcttccc aacctgataa
     3601 taattcttca tcgatttccg gaatatcatc gcgataaagt tccaaaacag tcacttcatc
     3661 atctggatga ttttctttat aacttgttaa aaaagacgtc aatgccttaa ctgtgcgcga
     3721 ttcttctgca gttaggggat gacctttgac tactaaaaca gatgccatat gcttaccttc
     3781 tccttttatt gctatgattg ttataactat gactaacaaa atcagtttac tcgaaaaaga
     3841 aaaggctatc aagtcatatt gataaggttg aagaataaat aactaaaaaa agacagggta
     3901 taaaagaggc tgtgacacga ttttttgtca cagcctcttt tgaatctaac aagcaatatt
     3961 caagcaacac tcgactgaag agcttccatc ccaccaatga tataaacagc acgaaaacca
     4021 ttagcagtaa gttgatcggc taatgcttct gatcgcctgc ctgtatagct tagaatataa
     4081 taaagtttct ttggatctag attttttaat tgtcttggca agatagcagc ggggatagaa
     4141 agcgagtggc ttaagtggtt ttttgtgtaa tctaaggtat ttcttgtatc taaaacgatg
     4201 ctgtcagcat cgagaggcaa tttattgaag tcttggacac taattgattg ggccatttat
     4261 ctcatccttt gtttagtatg tggctatttt aatgggcaac tctcattaat tcaaatggaa
     4321 ggattcctct tatcactttt ttaatgtatc ggatgataat gaaaaaaact atgcaaatgt
     4381 cttgcaaaac aggtaggagg tgttataatg acatagcgtc atatgacaat gtgtcatctt
     4441 gaaagaaagg agaatccata tgccgacaca gacatttttc catttgccta aagaaaaaca
     4501 aaaaagacta atcgaggctg cgagaatcga gttttctaga gtacccttaa aagaagcttc
     4561 gattgctaat atcgtcaaat tagcggatat ccctagaggc agtttctatc agtattttga
     4621 agataaagag gatctttatt tttattactt tgagacaatg agacgtgaca gctctaaaga
     4681 tatgttacaa ctaatgaaag aatccgatgg tgatctattc aaaggattcg aatcctattt
     4741 ttctaaaatg atttatgaga ttttggaagg cgagaatgct tctttttatc gtaacttgtt
     4801 tatgaacatg gattaccgtg cctcaagaaa agtcgctcct catacgagtc gccaaaaaac
     4861 gcctcacgat ccacataaag aacacaaaca agatgcaaaa gagttgcttg aattgatcga
     4921 tcggacgaag ctgaaagtaa aaaatgatca tgaattggaa ttactgatcc aattagtgat
     4981 gaatactgta tttacaacaa ttgcgcattc gtatcgctta ttttcaacat ctgaagatta
     5041 ctcagtagag caagcaatca gtgaattcaa tttgaaactt tcttggctga aaaacggagt
     5101 atataaatag aaagaaggga actagatgct aaaaatcgta aaacggatct ccttgacttc
     5161 agcaattgca gctgcagttt tcatggtgat ccaagtactg gcagatttat atttgccgac
     5221 acttacctcc aatattattg ataaaggggt agcgcaagga gatgtcgatt atatttggca
     5281 tacaggtttt gtgatgatcg gcttttcttt gatcagtatc ttagctgcta tcgcaaatac
     5341 attttttgct actcgtgaat cacagaaact gggtaagcaa ttgcgtacgg atgtctataa
     5401 aaaatcagag agtctgacaa aagatgcgtt tgataaatat ggaacggcct ctttgatcac
     5461 aagaacgacc aatgatgtga ctcaaatcca aatggttaca caaatgtttt tacgtatgat
     5521 gatcaatgca ccaatcacgt tgattggcgc gagcatactt gcttatcaaa aagatcatca
     5581 gttgacaaaa atcttcttag ttgtcatccc agtaatgatt attttgatcg gcggaatcat
     5641 gtactttgct gttccattat tcaaaagtat gcaaaagaaa actgaccgtt taaacctggt
     5701 atttcgagaa ggattgacag gggtcagagt gattcgagca ttcgataaga cacgctttga
     5761 agaaaatcgt tttgatttag cgaataaaga ttatacaaat acggcaatca aagttaatac
     5821 gatcgtcgca ttgatgatgc caatgatgac attgatcatg agcggtacaa acgtagcaat
     5881 cacttggttt ggtggacact acatagcaga tatgactttg gaagtcggta atctgattgc
     5941 atttatgact tatgcgatgc agatcttgat cagctttatg atgctttccg ctatttttat
     6001 catggtgcct cgtgcacaag cttcagctga ccgtattaat gaagtgttgg atgaaaaaat
     6061 cggtatccat gaccctgaaa atcctaaaac agtttctttt gctggaaaaa acgcaacatt
     6121 ggccttcaat catgtcaatt atcgatatca tggagcagaa aaacttgcat tggaagatat
     6181 tgatttccaa gccaagtcgg gcgagatcgt cgcaatcata ggggggactg gttcaggtaa
     6241 aacgacctta gtcaatttga tccctcgatt atatgacatc gaatctggtt cgatccaaat
     6301 aaatggaacg gacattagtg acatgactca atataatttg cgagaattaa tgggctttgt
     6361 tcctcaaaaa gcagttctgt tctctggaac gatccgagac aatatgcagt atggtaagcc
     6421 ggatgcaaca gatgaaatga tttggaaagc attagaaatc gcacaagcta aagattttgt
     6481 ttcagagatg gaagatggct tagatagcca tgtggaacaa ggaggaggaa acttctctgg
     6541 tggacaaaga cagcgtctgg caattgcacg ggcattagtc aaaacagcag atatctatgt
     6601 gtttgatgat tccttctcag cgttggattt caaaacggat gccaatcttc gccaagcatt
     6661 gaaaacgaat atgaaagaaa gtatcaccgt tttagttgct caacgagtaa gtacagtaat
     6721 ggatgcagat atgatccttg tgctagatga aggaaaactg gttggcaaag gaacacatga
     6781 agaactatta gcaacaaacg aaacttatca agaaatcgtt cattcacaac taagagagga
     6841 ggacttagca tgagtaaaac aaacacacgt ccctctcatg gaccagcagg cggtccagga
     6901 catggtggac ctggcagaaa tctcggtgta aaaggtgaaa agcctaaaaa tttctgggga
     6961 acggtacgcc gattgtttgg ctatatgtct aaacgtatgg ttgcgatcat cgctgtattt
     7021 gtactagcaa tagcagccgt gatcttccaa atccaaacac caaaggtctt ggggaaagca
     7081 accacagaaa tattcaaagg tgtaatggct ggcgctcaac agatgcaaca aggccaacaa
     7141 gtaacaaaat ttccgataga ctttgataaa atcgcgcaga tcattgcgac agtaatcgtg
     7201 atgtatttga tttctgctgt attcaacttt ttacagcaat ttattatgac acgtgtttcc
     7261 caacgaacag tttatgaatt acgtcatgat ttggaagcca aaatgaatcg tgtaccgatt
     7321 tcctattatg atacacatac aaatggggat atcatgtctc gagcaatcaa tgatatggat
     7381 aacatcgcaa gcacgctgca gcaaaatttg actcaatttg taactagtgc agtcacattg
     7441 attggggtca tcgtgatgat gctttcaatc agctggcaat tgacgttagt cgcgttattg
     7501 atgatccctt taagcttgat catcgtgatg atcgtagcgc cgaaatcaca agtgtttttc
     7561 gcagatcagc aaaaaagttt aggtttattg aataaccaag tagaagaaac atatggtggt
     7621 cataccattg ttaaaacatt caatcatact gaaaaagacc aggaagtatt tgaaaaagaa
     7681 aatcagaatc tgtatgccgc tggttggaaa gctcaattta tttcggcaat catcatgccg
     7741 ttgatgaatt tcgttaaaaa tctaggctat gtgttcgtcg ctgtactagg cggagtaaaa
     7801 gttgcaaatg ggaacatgac attaggggat gttcaagcgt tcttgcaata tacaacacag
     7861 ttctctcaac caatcactca acttgcaagt ttgatgaaca ctatccaatc aacagttgct
     7921 tcagccgaac gggtatttga agtgttggat gaagaagaga tgtccaatga aaaatctggc
     7981 cttccagaag aaaaagatac accatacaaa gttcgttttg aaaatgttca attcggctac
     8041 acaccagata acttgctaat gactgatttt aacttggatg tcaaacctgg tgagatggtc
     8101 gctattgtag gtccgactgg tgcagggaag acgacactga tcaaccttct tgaacggttc
     8161 tacgatgttt ctggaggtag tattcgttac gatggtgtag atacccgtga tctttcacgt
     8221 gatgaattgc gcgcacagtt ttcaatggta ctgcaagata cttggctgtt tacaggaagt
     8281 atctatgaca atatcaaata tgggaacgac gatgcaacag acgaacaaat cattgaggct
     8341 gctaaagctg ctcacgtaga cgattttgtt cgtaaattgc cagaaggata taacactgta
     8401 ttaaacgagg atgcaagcaa tatttcgcaa gggcagcgtc agctaatcac aattgcgcgg
     8461 gcattcttag caaatccaga tgtcctgatc cttgatgaag caacatctag tgtagataca
     8521 cgaacagaaa tactgattca aaaagcgatg aaccgcctat tggaaaaccg gacaagtttc
     8581 gttgttgctc accgcttgtc tacaatacgc gatgcagaca acattattgt gatgaatcat
     8641 ggttcgatcg tagaaacagg aaaccacgat acattgatgg aaaaagacgg attctatgca
     8701 gatctttata acagtcagtt ttctgaagaa gaagcttcat agaatagtac aaatttatta
     8761 ttttttttag gggctgtgac aaaagtcgtg tcacagtccc tttttccgaa taaacggtgt
     8821 tcaatcagct gtccttcggt attagctcaa aaaacggcaa aatatgagaa gctatttttc
     8881 cgttttgtat ctctaatact caggtcaaac cgctgatttt acaacctctt ccttaaacgg
     8941 tgttctcctg ccaactcctc ggtcacaagt cacaatagtc aacaaacctg agaagctgtt
     9001 ttttgaaatt gctccttgtg actcggagtt aaacggcagg ctcacaacct cttggtaaac
     9061 ggtgttcaaa agccagtttc tcggtattaa gccaaattta tgcaaaaatg tagaaagcca
     9121 ttttcacaaa atttttctta ctactcgaaa ctaagcgact ttttcacaac ctcttcctta
     9181 aacggtgttc aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aataggagga
     9241 gctatttttg tttggctgtt cttattactt gatgcagaac agctttttca caacctcttt
     9301 ttataagagt ttttactcat taatttacaa aaaacaagca aaatgcgaat tttctcacat
     9361 tttttttaac aaaatcaaag ttattgcttg aaagaagaag aatccatcgt taaaataaag
     9421 cttgacgtag aggggagcaa gaaatatgaa agattttcaa ttgggggctg ccacctattt
     9481 aaaaagtgta gccattcggg tgaaagaccg agataaaatg attgattttt acaagaacgt
     9541 tgttggcttt gatttgaaaa gagaagaaaa cgaattagcc attttaggaa cagaagaagc
     9601 tggaagtgaa atgttgctgc tggaagaaag tcctagagcg aatgatcaca tgggggtgat
     9661 caaaaagctg aaccgttttt cattgattat ccctagcaca gaagaaatgg cagatatcct
     9721 ttgtcgtatc cgtaaaatga attatccaat caaagaagct ttagaagatc atggacgtat
     9781 ggggattttg ctcgtagacc cagaagagaa tgaattggaa atttaccatg aagagaataa
     9841 gaacgaccaa agagaaattc cagaaacaat ggatcaagaa gcattattaa gaaaatctga
     9901 agaaaaattc agtaaactat caacaggttc ttattttgaa aaaatccatt taaatgttac
     9961 taacttaaca aaagaacgcc aatttcttaa tcatgtactt ggtttgaagg tacataatga
    10021 agaagagggc atccatgtat tgaatgaagg agatttccat gttggtttga cagaagctag
    10081 cggaggcgca attgatttgc ctacagatga agtgattgga cttgattttc tgaaattcat
    10141 gatcgatact gaaagcatgg aaggactgat tgctcacttg cagcaactat cagttgattt
    10201 cttcttagac aaaaagcaca cagtactcac aatctatgat gctataggaa tcgaatggtg
    10261 gttcgtcagt gagccaaaag aaaagaataa aactgacatt cgatgcgacg cttaactttt
    10321 tctttcttaa cgattgtcgc atgtctcgtc attttatgaa cggtcatact atcgcttatt
    10381 ctagagatag aaagagaaaa tagtataccg ggggaaactg cgcggttttt cgcgcagttt
    10441 ttttgtgtaa aaaaggttga ttcctcatag attaagcttt aagcttggac tgtaagaatc
    10501 tgatagaaaa gcaggagaaa aagatgtatc cacacacgaa acagcttatt ttgaaaaaga
    10561 tggaagaagg attttatcca ggtgtagcag tcgccttcat aaaagaaaat caagtagaaa
    10621 catttacagc cggctttgcc caagtcgttc ctgaaaagca gctgatgaca gaagatctgt
    10681 tgtttgatgc agcctcactg acgaaagtcg ttgcaaccac ttcattagtt ttacgtctat
    10741 tagaagatgg aaagatagaa ctcgatcaac cgcttcataa atatcttcct gaatttgaaa
    10801 atcagacaat cactttgcgt catttactga cacatacttc tgatatcaaa acatggatac
    10861 caaacagaga taagctatct caggaagaat tgatcgccgc ttatttgaag ctagaaccag
    10921 gtgagcaact aggaaaagaa gtaaaatata cagatgccgg tacgattctt cttgggttga
    10981 tgctagagga aatttaccaa gaaactgcag tggaaatctt tcaagaacaa gtattggaac
    11041 cattaggaat gatgaacagc ttatttcttc cttttccttc caaaaaaatc gttcccacag
    11101 aacaactgcc ttcgggtgag gttttgcgtg gacaaactca tgatccgaaa gcaagagttt
    11161 tggctgaaca tgcaggtaac gcgggtctct ttattgattt gaaagacagc attacttttg
    11221 tccaaatgta tttgaacaag ggaaaaatcc gtaatggtcg ttttttagaa gaagagacga
    11281 ttgattcttt actgcaagat caaacaccga aaaaaaatgg ctatcgttct ttaggatggg
    11341 acttaaaaaa ggatgtagaa gacggtcatg cgattctttt tcatactggt tatacaggta
    11401 cgtttttctt gattgatata aaaacacaag aagcgttgat atttttgtca aatcgcgtac
    11461 atcctgtcga tcatcgtgcg gagtatctca tatgtcgaga tgaaattgtt gaaaactttt
    11521 tgaaagagaa agcggtatta ccgaaataat gctataatag ctatgaaaaa tgagaggagt
    11581 ttgaattatg caggaaccta tgtttttaaa accagttttt caagaaaaaa tttggggagg
    11641 aagtcgttta cgttcagtct ttggttttga tatccctaac gataaaatcg gagaagattg
    11701 ggcgatcagt gcgcatcctc atggtgtcag tgtagtagaa aatggcgaat tcaaagggaa
    11761 aagattagat gaactatggt cagaacacaa ggaattgttt ggccatcctt cagaaccagt
    11821 gtttccatta ttgatcaaga tcttagacgc agaagatgat ctatctgtac aagtacatcc
    11881 agatgatgcc tatgggatga aacatgaagg tgaattagga aaaactgaat gctggtatat
    11941 catcgatgct gaacctggtg cagaaatcat ctatggtcat catgcaaaga caagagaaga
    12001 actagctgag atgatcaaag atgggcgctg ggatgacctt ttgaagaaag taccagttaa
    12061 aaaaggtgat ttcttttatg taccaagcgg aacgatccat gctattggta aaggaatcat
    12121 gattttagaa acacagcaaa gttctgacac gacttaccgc gtgtatgact acgacagaaa
    12181 agatgcgcaa ggacaaacaa gagagctgca tatccaacaa tcgatcgatg taacaactgt
    12241 ccctgcgaaa acaccagaat tacaaatcaa agaagtccga aaagggaatt catcgatcgt
    12301 cacttattta gaaactgagt ttttcaatgt atatgagtgg gatattaaag gaatcacatc
    12361 tttcaaaaaa caagcccctt atacattgat gacggtaatc gaaggagcag gagaccttgt
    12421 cgttgacgga aaaacatatc cattagaaaa aggaacaagc tgtattattc caaatggcgt
    12481 ttctgagtgg gcggtccaag gtgaactagc aatcattgcg tctgaaccag gcaaatagtt
    12541 cccagatagc tgaaagcagc agacggtttt tcgtttgctg ctttttctac ataaaatccg
    12601 tccatcaata aatagaagtc tggtgaaaaa gatggaatac aaatggaaag caaacaacag
    12661 cagtcgcgtg tctatgaaac aactgagaaa agctggacat cacttaaatg aaatgatcat
    12721 gccaaatcta agtctgttta tcgcttgggg aatcttgtca ttagcaacaa aaggtgtgtc
    12781 tggcgatttg cacaccacat tagaagaggt atgccattgg atgatccagc tgcttttacc
    12841 catcttgatc agttattcag gcgggaaatc ggtaggggga caaaaagggg cagtcgccgg
    12901 tgcaattgct tctttagggt tgatcgctcg ttccaatgct ccacaaatcg taggagcgat
    12961 gattatcggg ccgcttgccg gcatcgtata tgaacaattt gtttctagat tccatgtgaa
    13021 attcaaggct ggatatgaaa tgcttgtcag caacctacta gtaggactga ctggtagcgt
    13081 tatttgtata attggaatcg tttttattga accagtttta aatagtgttc acttgttgct
    13141 cgcctccgtt gtgtcttggc tagtcacaat gaatctgtta ccacttgtca gtctgatctt
    13201 agaacctcta aaagtactat ttttcaataa tgcaatcaat catggtgtat tgacaccttt
    13261 aggaatcgaa ttctcacaaa cagtcgggta ctcttcctta tttttgatgg aggcaaatcc
    13321 tggacctggt ttagggatct tactttctat cctttgtttt gcagaaaaac aggaaaaagt
    13381 aaatgcgagt ggagcattga tgatccatgc gattggcggg attcacgaaa tctattttcc
    13441 atttgtcttg ctgcgaccgt atctattttt agctgtaatg gctggtgggg caagcggcac
    13501 gttgatattt caatggttag atacgggatt agctgcacct gtatcgcctg ggtcactact
    13561 cgtgattttt gccaatgcgc cttatccaca actagctggg gtaagtatag ggatagcaat
    13621 cagtacattt gtgaccttca tttgtgcagc gatactattg aaattaccaa ataaagaaaa
    13681 agagaacaaa acggtcaaag aaaggggaga aatcttggaa ccatctatca ttcattccgt
    13741 catttttgct tgcgactcag gtatgggatc aagtgctatg ggagccgccc tgctgaaaaa
    13801 gaaattagca gcagaaaaaa tcgatctttc tgtcggatac agctctatct atgaattgaa
    13861 taaccaagta aatcagctgg ttatcgtgca acatgaacta gcgggtcttg caagagaaaa
    13921 agtacctcaa agtcaaatct atgtactgga tcactttttg gaaacagata tcttgctgtc
    13981 acgaataaaa gaagcgatcc aatttccaaa gcgggaagaa aaaaaagaga tagcagatat
    14041 cacagaacaa aaaacgtcgc ctgctaatat tgtatttttg tataaaaaca acagacgcgg
    14101 ctcacaaact atgggcatga cgattttaag tcaattagca aaaaaagcga aaaaacagtt
    14161 tgttatctca aaagaatcaa tagaaaaaat caaaatccaa gaagaaacga tttatatcgc
    14221 acaaagagaa ctagctgtaa ctgaacagct agaagaaaag atccctcggc tagttctagt
    14281 tgaacattgg gtagctacga atgaatacga aaaactcgtg aagggagaaa tataagatgt
    14341 atttatcgcc cagagaaaaa caattattag cagaatttct cagtagcccg tcacctgtat
    14401 ctatccaaaa aatgatgaat ttattgaagg tcagtaaacg gactatttac cgcgaattag
    14461 ataatcttga agtctcattg aaatcggtaa atgcttcttt gaaaaaagca gctagaggct
    14521 catttgtcat tgaggcatca gaagaagtat tagagcaatt aaaagaaaat acagatggga
    14581 aaagtaatga cttatccact gctaaacgac aacacgcgat tttagccgag ttgttactga
    14641 cgacagagcc gttgagtctt gctcatttca tggaatcata tcgcatcagt aataccactt
    14701 tttatgcaga tatcaaacag ttggaagaaa gtattcgcca actgcctttg gaaatcattc
    14761 ggaaccaggg atacgagatc gaaggaccgg aaaaataccg ccggcttctg acagcgaatg
    14821 tattagaatt agaaatcaat gaatatgaac tttttcatag tatttctttc gatgcatcac
    14881 tgaattattt ttttcgattc gttgatccgc aacatctatc tcttgcacgc aaagtaatcg
    14941 gggaagaatt gatccaaaaa aagaccaatc taagtgatag aaagcttgaa catttggttt
    15001 tgatgctgac attgacgatg gagcgtgtga cgaaaaatca tttactgaca aatgagacat
    15061 ataatagtct ggcaaacaaa gaattactac agacagctaa acgattgttt tcacggatcg
    15121 cttctgagac aaaacaactt tatccagtaa atgagatcgt cttttttgca ggattattaa
    15181 atgacttcac gaactcattt gatcagaatt tctttgaaga gacgttcgat acacagcttg
    15241 cttatttagt aaaacaactg attcaagaag taagtgaaga gacgaatgtc catttttatg
    15301 aagatgaaac tttgtacaaa atgctgttga ctcatttatc tggtgtgttt tcaagagccg
    15361 ttttacaaga agagacactg accaatccga ttttagaaaa aatcatggtt caatacgagg
    15421 aagttgcgaa tgcaatccgt aaagcagtag agcggatttt tcaagaaaaa aaattatccg
    15481 aagaagagat cgcttatatg gtccttcatt ttgcgaattc attggaacga agtccgaaag
    15541 aaagcaatat cagtattgca ggattttcgc caagtggatt agcttctact agcatgttgg
    15601 aactacgatt gaaaaggtat tttcccttca tccaacaaat tcatttctat cggattgctg
    15661 atttaaaaaa aatcgattta caagaagaat atgatgttgt tatttctaca tctattttac
    15721 caggatacgg tggaaaatat attttagtct ccccgctact gctagaagat gaagtcaagc
    15781 agctaaaaga agctttttcg catattgaga agaaaaaaag gcaaaaaatt acgtataaca
    15841 gcaataacga gtggatgata gaagatactt atgaggaaac aatgcatttc atggatcaaa
    15901 tcaatcaatt actcggtcgt ttttttatcc aagaactgga taatcctgag acgatttccg
    15961 aaacgattcg cagcttattt ccttattttc ctgaaggact agtaacggat gaagaggtgg
    16021 ttcatcgccg gctgcttaag cgatatgaac aagcacctat cggtattcct catacaaata
    16081 tggggttatt ccatacatcc agctcattga tttctaaacc gatcttttgt attttcaatt
    16141 tgaaacatcc gttgaccatt gaagggatgg atagacagcc agtcgatttg tcccgtatgt
    16201 tagttatgct ggcgccttcg ccaatccaag aaaaagacgg gattctttta ggaaagatca
    16261 gtggagcaat catcatgaat gacttgaaca ccgagatctt ccgctcagga aataaagaaa
    16321 tcgtttatca gttgttagcg aaaatcctga ttgaagaaat aaagaaatga aggtgaaaag
    16381 atgatacagc taccagcaga aaaaatattg ctcgatcaat cctatcaaaa ttggaaagaa
    16441 gcgttgaccg agatcggcca gtggatgacc caacaagggg gaatcaaacc ggcatatatc
    16501 caatcaatga tcgaaagaca agaaaaagcg agtgtctata tcgggaattt cgtagccttg
    16561 ccacatggga ctgacaaaga tgaagaagta gtagaagaag gaatctattt agtccaagtg
    16621 cctgatggtg tggactttgg aacaaaagag gaaccaaaaa tcgcgacact cttatttgta
    16681 gtggctttaa aaaaagaaca tcagttagcg gtgctccaag atttagcctt tttctgttca
    16741 gacatcgacc aagtaatggc gctcagtgat gcggaaacca ttgaagaagt ccaaatgatt
    16801 tttgaacatg gacatatata accaatgttc acagtaaaaa aagctgcctt gcatcaagta
    16861 ataacttcaa ccaagataaa aataagagga gctatttttg ttgggaggtt cttattactg
    16921 cggatgcaag cttttgaaaa ctatttattc tagaaaaccg tttatttgaa ataggggacg
    16981 gtgacaaggt ttttgtcacc gcccctatta tttttgtgtt ttaatagctc ttttttatat
    17041 agatagtgcc catacctatg aataataaaa cgatactcca tataccaagt gtcaagatac
    17101 cattcatgaa agtaacctgt tctgaagcta gtggcgagaa aatatctcct cctatgatca
    17161 cattttgaaa atcaacataa gaagttggca agtaatgact gattgattct atccatccaa
    17221 tcgaagaaaa caagcgatac tctgctaaga aaagacaaag aagtgtacct aaaagattga
    17281 ctagagaatg ttcaaataaa gcagatagaa gaaaactgat tgtacttaaa aagagcaccc
    17341 acaacaacag gaacagaaag ctctggaaaa gatactcata catcggcagg attcgtatct
    17401 cctgaccaat gatcgttcct acaggataac ggaagtctcc caaaccattt ttgattatta
    17461 ctagtataga gacgatcaaa aatggcatca cgagacttag catagctgca ccataataaa
    17521 tggtaaaacg gatgctatgt ttctttacca tactatccag taatagattg actagagaga
    17581 ttacctgctt tcttttttca taagagatga tggaagaaag aaacaaggcg gttatacata
    17641 aaatcaaaaa actagaaatc attcctgaaa aaatattttc gtagtaattt gctaaagaga
    17701 gatgcgctgg caagtcaaaa atagaatatc tttggatctg atgttctttc atataacgga
    17761 gcaactcgac acgtttttgt tgttcaatga ttggaattcc ttgtctttga ccagaaatca
    17821 gtcgatccag tatatctttt tcataatgat aagttgcttc gaccatttgc tggacattcc
    17881 ctgtgttttt tgcatgtaag attgcttcga tcagtttgtt gcttttctct aaactttcca
    17941 caaattcttt ctcattctca tcttctttca acgtttcaat caaacgttgc atctcttcat
    18001 agttatgatt cagttcatcg aattctgtat aagcaagttt atgatcaaaa aaaagtggga
    18061 atgcaaagag cacgaccaac aatagaggaa ataaaaagtt tactttgttt tttagtaaga
    18121 gattgcgttc aaatgtaagt aaagacatag ttacacctca tataatttac gataaatagc
    18181 taaaatctct tccatatttg aggattctgt gacgatttct ccatttttta gaaaacatgc
    18241 tttagaacaa atcgattgga tattcgcgat agcgtgggaa gaaagaagaa tcgtcttccc
    18301 ctctttctgc cattgtctga tgaggctatt caagatatca atacttcctg ggtctaatcc
    18361 gttcaacggt tcatcgaata acatgacaga tgaatcactg atcaagtaag cagcaagcaa
    18421 gacatgttgt ttcattccta gagacatttt tccaatcggc aaagtaaggt actcagtcat
    18481 cttcaatcgt tggaaaacag tttggcattc tactttgctc tgccaatagt atttgatcaa
    18541 agtcaaataa tcaacagccg atagatagtc ggataactga ttcgttgatt ctagaaaaaa
    18601 gaaagaccgg cgataatctg tataagcagt aggagagatg ttgtgaaggg acaattgatc
    18661 ataagttgtt ggcagcagtt gggcaatgcc attcagcaaa gttgttttgc caaatccgtt
    18721 cggggcaatc aaagcagcta cttctcctga ttctactaaa agattcaatt gtcgaatcac
    18781 acgtttttta ccacgtttca tgttcatgtt tttgatcagt aatgtcatag ataggcctcc
    18841 aatataaaat ttaataacat aatagaatgg aaaacatcta aattaaatgc taacaaaagt
    18901 tattgtttgc atattataaa aaaattaaaa taacagtttt atttaaaatt gaagttttct
    18961 gataccgaaa tctttttatt ttgctgacat actcaaacct ttggcacaaa gtttcgtgcc
    19021 aaagtacgac tatttctctt tttagtaagc ggatacaatt agagtatcaa aaggagagag
    19081 ggagagacaa aatggaacgg acgattccca aaaacaacat ttcgctaaaa gcgagggtac
    19141 aaaaattagg cagtacttta tcaagcatgg tcatgccaaa tattggagca ctgattgcct
    19201 ggggtgtatt aacggctcta tttatcccag atggttattt accaaatgag gctttcgcta
    19261 ccatggtaag cccaatgcta acttatctga ttcctttatt gatcggttat actggcggaa
    19321 aagtaattgc tggtgaccgc ggatcagttg ttggggcaat tgcaacgatg ggtgtgattg
    19381 tcggaacaga tattccaatg atgctaggcg cgatgatcat gggacctctt ggtggatatg
    19441 ccatcaagaa atttgatcag ctattccaaa aacggatcaa atccggtttt gaaatgctag
    19501 tcaataattt ttcagcaggt cttatcggtt ttggattagc gttattaggt ttttctgcga
    19561 ttggtccagt tgtcgatgca ttgactcaag caatggcaaa aggtgtagag atcattttat
    19621 ctgcccattt gattccattg acaagtattt ttattgaacc agcaaaaatc ttatttttga
    19681 ataatgcaat caatcatggg attttgacac cattgggaac tgaacaagta gcagaggctg
    19741 gaaaatctat tctattcttg cttgaagcaa atcctggtcc aggacttggc gtattacttg
    19801 cttttacttt gtttggcaaa ggagcagcaa aatcttcagc accaggagcc atgatcattc
    19861 actttttagg cgggatccat gaaatctatt ttccttacgt tatgatgaaa ccgttattat
    19921 tcttatcagt tataggcgga ggaatgtcag gaagtttcat tttccaattg atggatgctg
    19981 gactaagagc gccagcttct ccagggtcga tcattgcgat tctagcgatg acaccgttca
    20041 attcttacct tcctgttatt ttgggcgtga ttgttgccgc agctgtttcg tttggtattt
    20101 ctgcagtaat tttgaaagcg gatgcgaaag atacaaatga agacttcgaa aaatctgttc
    20161 aagagacaaa ggcagctaag caagcttcca aaggtgccca aggtattcct caaggagcta
    20221 cattctctgg cgtacaaaaa atcatttttg cttgtgatgc aggaatggga tcaagtgcga
    20281 tgggcgcttc gatcttgaga aaaaaagtac aggaagcaga cttgcctcaa acggtgacca
    20341 atcaagcaat cagccaactg acagatgact cgaatacatt gatcgttact caggtcgaac
    20401 tacaagaaag agcaaaacaa aaagcaccga gcgcgcgttt tgtctcagta gaaaactttt
    20461 tgaattcacc tcgatatgat gaaatcattc aagaattaag cagcaagcag aaaacatcag
    20521 tttctgaaat tgagaatgag ccagcagaag agtttctttc agcaaacatc aaacaaatca
    20581 aagagattct acttgtccac gatgatcgtt ccggatcagc aacaatgggg atgaccgtgc
    20641 tcaaagatat attagacaag aaccatttaa ctatcccgtt aagaaaagtc aatatccaag
    20701 atttaaaaga tgaacctgat tcactgatcg tagccaaaga aagtttagcg gaaaaagtac
    20761 agatggctgc acctcaatcc attcatttat cagtcaacag catggtcaat ccgcaaaaat
    20821 acgaatcgat tgcacaatat ttgaaacaga cagcttaagg gaggaaaaca gcatgcaaag
    20881 tttgccattc gacatgatcg agctcaatcg atcttttaaa accaaagaag aagcgatccg
    20941 ctattgcggt cagatgcttg tagaagcagg gtgcgttgat gaggaatata tcgatgccat
    21001 ggtagataga gacaaaatgt tatctgttta tatggggaat tttatcgcga ttcctcatgg
    21061 aacagatgaa gcaaaaaaat atgtgaaaaa atccggcatc tgtgtcgttc aagtaccaga
    21121 tggtgtagat tttggtaccg aagaagaaga aaagattgcg actgttttgt tcggtattgc
    21181 aggagtcgga gatgagcatt tgcaacttgt acagcaaatc gcactttatt gcagtgatat
    21241 ggacaatgtt gtccagctag cagacgcgct cagcaaagaa gaagtaacgg aaaatctagc
    21301 aattgcttaa ggagagagaa aaatgagagc agtacatttt ggtgcaggaa atatcggtcg
    21361 aggatttatt ggagaggttt tggcagctaa tggatttgag attactttcg tagatgtgaa
    21421 tcacacgatc attgatgctt taaatgaaag aaaagaatac gaaatcgaat tagcagatga
    21481 atcaaaagaa aaaatcactg taaaaaatgt caaaggaatc aacaatcaaa caaatccaga
    21541 aaaagtaatc caagaaatcg ctgaagcaga tttagttact accgccattg gcccgaatat
    21601 cttacctttt attgctgagt tgattgcaaa aggtatccaa gaaagagaaa aggaaggaag
    21661 cacaacatct ttagatatca ttgcctgtga aaatatgatt ggcggttcag catttctaga
    21721 aaaagaagtg tataaatatc ttccggaaac atcatttact gacaaatata tcggctttcc
    21781 aaatgcagca gtcgatcgaa tcgtcccatt acaacaccat gaagatcctt tattcgtcca
    21841 agtggaacct ttcaaagaat gggtcattga cgatagccaa agaaaaaata aaaaaatcca
    21901 attaaaaggg gttttatatg tagatgatct agaaccatat atcgaacgca aactatttag
    21961 tgtcaatact ggtcatgcga ctgtcgctta tacaggtgcc ttgttaggtt accagaccat
    22021 tgatgaagca atgcaagatg cgttagttgt ggcacaatta aaatcagttt tgcaggaaac
    22081 aggaagtttg ctgattgcca aatgggggtt tgatgcagag cagcatcatg cgtatattga
    22141 aaaaatcatt catcgcttcc aaaacaaaaa tatctctgat gcgatcacac gtgtcgctcg
    22201 gacaccatta cgtaagttag gttatcaaga aagatttaca cgtccagtga gagaactaca
    22261 agaacataac ttgacttgcc cacatttgac ggcaacaatg gggattattt ttaattacta
    22321 tgatcctgaa gatgaacaaa gccgtcaatt gcatgaaatg aagatccatg aaaacttgga
    22381 acaattgatt caagaagtca caggaatcaa tgatccgaaa acaatcggga atatcaagca
    22441 aaacgtgaac cgttatgcca agcaagtcgc ttaaaatagg atacgaaaaa ggggaatgtg
    22501 acgaaatttt tgtcacattc ccctttttcg aataactagg ctgtttgtcc ttcaagatag
    22561 ggcagatcca attctttacc gtaacgttgt tctaaagcta ggcggatcaa acgagctgcc
    22621 aggttctcat ttcttgcaag gataggtcca tggaaataag aaccgaagac atttcggtaa
    22681 atgacacctt cagattggtc ttcaccatta tttccttgac cttgtaccac tttccccaaa
    22741 ggtcgttcac cttcacctaa gaaagtccga ccattgtggt tttcaaatcc ataataagtt
    22801 tcattgaatt cttcgttatg gataacgata tcaccgataa accggttatt ttcctgactt
    22861 aacgtgtagt gatccagtgc actgattcct tggatctttt ctccattagc tcccatgtag
    22921 tagtgaccta gcaattggta gccaccacaa atggctaaca tgacgccatc attttcgata
    22981 tagttgatca aagaatcttt ctttttctgg atgtcttttg aaatgattaa ttgctcaaag
    23041 tcttgaccac cgccaaagaa aaccaagtca tatttatcag catcaaaagg ttcatgtata
    23101 ctaacgatct ccgtatggca agaaaccccc attttttctg ctatatattt caacatcaaa
    23161 aggtttccgt tatcaccata tgtgttcatc aaatttccat aaaggtgggc gatattgagt
    23221 tcataattag ccacggttca ttcctccttt gataaatcct tgagcagcta gttctttacg
    23281 aagctgcaat acagcagtgt aagtagctaa aatatagaca tgctcagttg gcaattcttt
    23341 gattttttcg attacttctg gtaattcttt tgtttctgtc aatttatttt ctggaatccc
    23401 ggcaactttt agacgtaaag tcatatctgt atgacggtct cctccagaaa taacagctgg
    23461 gatatccata tcggcaaagt tttcaaaatt accatcccaa atccagctga catcgattcc
    23521 gtctgcgtaa ttggcattta ataaagccac taaagaaaat ggataaggag ctagaccaat
    23581 catatcgatt acttgattga tacctacagg atttttgaca agtactaatg tacatttttt
    23641 cccattgata ttgatgactt cttgtcggcc gaatactttc tcgtcatatc ctaaaccttg
    23701 tttgatttta tcaggatcga cattgaagta ctctgccact gcagtagctg ctaaagcatt
    23761 gtagacatta tacataccgc caacttcgat tccgtattca tgaccatcga tcacgaaatc
    23821 tgctgacaca ttgtccatcc gtaccatatc agttaatcga taatccaatt caggacgttt
    23881 gaagtcacaa tttggacagt aatatttccc taagtttgca taagtgatca ttttataatg
    23941 caagatgtga tgacaatttg gacataagac accgtctgta ttgtaatgag ccatttgttc
    24001 acgatcttct tcatggtcaa agccataata ttttctagga ttgactgttt cgatagaatt
    24061 gaagattggt gaatcaccgt tactgataat cgttgcattt ggtgactttg cggcaccgtc
    24121 tacaatcatt ttataggtcg tatagatttc tccataccga tccatttgat cacggaaaat
    24181 atttgtaaat aagaacagtt ctggtttcat atattctgtg actttgctca aacttgcttc
    24241 atctatttct aggacagcaa actttttctg tccttttttt ggttttgcct gcaagaaagt
    24301 cgatacaatt ccctgtacca tattcgcacc agtagggttg gttagaactt catcgaattg
    24361 ctggcgcaaa atattgactg ttaaagcggt agtcaatgtt ttaccgtttg ttccggtgac
    24421 aacgacgact tggtagtctt ttgccaaact atctagtata tgtggatcga ctttcagcgc
    24481 tagtttacct ggtaaacttg atccgccttt aaaaaaagtt tttaatgccc attgcgacgt
    24541 ttttccaact gcaatcgcaa aatgactgcg tattcccatt atgtaacctc caaaaacgat
    24601 ttgctgacga ataaattcaa ctaatcatac catatgttga aaaaaaggtt aaattgcttt
    24661 tcttattctt aagaaaaggt tacggctgaa agtgcatgaa atcggacaaa atcgcatgat
    24721 ttaacaaaaa aacagcttga aacaaaatga ttgtttcaag ctgctgtgat tttattattc
    24781 cctcttacct atattcccat tttaaccgat aataattttc tcgcttggat attcatagcc
    24841 tagatctcgt gtttcttttg gcacgaataa catcaatgta tatagcattc cgatccgccc
    24901 aataaacatc agtgtagcaa tcgtgatttt gcccacaaca gacaagtctc cggtaatccc
    24961 taaagataaa ccagttgttc caaacgcaga agttacttcg atgatgatcg agatcagcgt
    25021 ttgttcttct gtagcagata aaaaaacgat acaaaagaaa cacataccaa gagaaagcat
    25081 aaagacaacg actgattttc gcacatcatc ttgatcgatt ctccgtccaa aaatattgat
    25141 gttatcttca cttttgagga aggaatatag atacaaaccg atgattgcta cagtagttgt
    25201 tcgaatcccg ccgcctacag aactaggact gcatccgata aacatcagta aagaaaaaat
    25261 gatcagtgtt gtgatttgaa aatcacccaa atcgtgaatc tgcagaccag cattacgagt
    25321 cgtaatcgaa taaaacatcg aattgatcca tttgacactc atattggaat cttggaataa
    25381 atggtccttt tccaatagat aaatcaagac agttcccgaa acaaacaaga taacaaaagc
    25441 taacacagct aatttggtaa acaaggaaaa acgaaacgga agcttcgcat tggaacgttt
    25501 gtataatagc cattcccggc actccatcaa tacaggaaaa ccaattcctc cgacaaaaat
    25561 caaaaacatg atcaggatca agaaaaaata gtcatgtgca aagggcttga tggaatcccc
    25621 agtgacgtca aacccagagt tcgtgacagc cgatatcgcc tgataaaaac cgtaaaaaac
    25681 ggcatcacgc caacgatcaa aatagcctct gtaataaaaa taaatagaaa agaatgtccc
    25741 aaaaagtaat tgaaaccaga ttaaaatagc aaaagtgatc cgaatcagtc ggacgatacc
    25801 agacaaacgc ggttgattca tatccgtcat gatcagctgt cgctgtttca gtgtgatccg
    25861 tcgtttagaa aaaatgacaa aagcagtaga aatcatcatg atccccaaac caccaacttg
    25921 aaataatatt tccagcagta taataccgtt atcattgaac acagaattga tatcaaaagt
    25981 cgatagtcca gtcacactca ctgtactgat tgccatgaac agcatgtcca caaaagaaac
    26041 atggctatct ggttcgcgaa aaatcggcat ataaaataga acgagtgata agaccgtcat
    26101 caatatataa taaaaaacga tgatttggat cgatgagaaa tgattggcag caaacttctg
    26161 tcctcgccgc tgcagccttc gaaagaataa atcaacatgc acagtactat cctcctgaca
    26221 atagtcgttt ctaccagtat acaaggggaa ttaggaatac tcaaagaaat tatcaaacag
    26281 ataacaatac agctttcgat taaaatagaa atggcttata ataaggaaaa tcctagaaaa
    26341 aagaaggagg aggaataaca aattgattaa tgctcaacaa atcgtagaaa aaatgaaaaa
    26401 tcaaaaaata aattatgata cagttctgaa aaaaatgatc atgcaatggg agaaagaaga
    26461 agtaaagcct aagatattaa tacatacatg ctgtgctcct tgcagtacgc acacattgga
    26521 attcatgtct caatatgcag agatcacgtt atttttttca aattcgaata ttcatcctaa
    26581 aagtgagtat ttgcgaagat tacatgaaca aaaacgattt gtagaccaat ttaatgagaa
    26641 aactgaccat catgttgcgc taatcgttga tgaataccgt ccaaatgaat tcaccaaaat
    26701 ggttttaaca aaccatcttg aaaacgaaaa agaaggtggt gctcgctgca gtgcatgctt
    26761 caatatgcga ttagatcttg ttgcgcaaaa agcgcaagaa ctaggatacg attactttgg
    26821 aagtgccctg accatctcgc caaagaaaaa tgcagctttg atcaatcaga tcggaatgga
    26881 tatccaaaag atttacggga cacaatactt gccaagtgat ttcaagaaaa acaaaggcta
    26941 tgaacgctct ttgcaaatgt gcaaagagta tgatatttat cgccaatgtt attgtggttg
    27001 cgtgttcgct gccaagcagc aaggatgcga tctgaaagaa atcaatcggg aagcaaaagc
    27061 ttacttaaaa acatcaaccg tagtttttga ggaaaattaa agaaatcttt cggaaaaacg
    27121 taagaaaaaa ataaagaatt tatggtagaa ataaggtaga ctgtataatt tgtccgtagt
    27181 aaagtaagga aacaagaata aatttcaatg aaggaagaga taagatgcga aaagtagcac
    27241 ctattttgaa gccaatcgct gtattaacaa tctcgacgat tgtcgcgact actacaggta
    27301 ttgtgaatag ttccagctac cgtgcaccaa gttcaagttc tagtactgta gaagaaacaa
    27361 caccaactac ttcagtctcg acatctgaac caacaacaac ggaaagtttg aatgaaacca
    27421 ctacgacaag tacatgggac gatacaacaa ctagttcgtc gtatgatgag acatatacaa
    27481 ctgacagctc aagcgaaaca agtaatacgg taccagatgg cactccatcg gattcaacca
    27541 atccaagtac aagtccggat acaagtgcta gctccgaaac gataccaagc agtacgactg
    27601 tagatgataa cacgcaaaat gatgatgcag cacgtacagc acagcaaaac caagattcag
    27661 gaaataccca aacgtataac cagcaagcag gtgattctca atgacatttt tactagacct
    27721 tatcaacggg gtgcataaat tccttggcta tttagacatc agtcctaaat atcttaaccg
    27781 tggatatacg attttaagtg tgattcccac gctttattta ttgagaattg tttatggact
    27841 ttggcaaaac caaaactatc ttcaattttt cttatatgga cttgcatttc tagtgcttgt
    27901 ttattttact gttttaaatg tattttatta ctttttagat aaaaacacga aagccgatgt
    27961 cactcagctt tttgtgaagt atttacctga tgaagcgttc aacatccaga cagaagaatc
    28021 gatagataat ggaaattcga tcgataaagt gaatacacaa gaggtcatgg tttcttatga
    28081 cgaagattat caattgaaat tagccgagaa tatgcggtat ttgattggaa atggtgagat
    28141 caaaacaaat gaactcggtc gcatggatgg atttctggtc gatagaaata cattgtatcc
    28201 ttattactat gtcaagaaaa caggagagaa agagtatgtt ctaaatattg gacgaagcta
    28261 ctcggacatg gaaaaagtag ggaccattcg acttgactca acagaagaca cattggaacc
    28321 tgtcggattg ttcatcgtcg gcggtgattt tgtcaaagac gggttacgct atcatgagcc
    28381 ttatcgtctg aaattgattg caaaaaaggc acagtcgcag gaagaaccag tggtttatag
    28441 tcgtagccaa agaagaagac attcataatg tgatgaaacg ccgtgtatca gtggttgcga
    28501 taaaaacgtt tattcggaat aagggggatg tgacacgtct tttgtcacgt tcctttttta
    28561 tataacgaga aagataagac gaacacatca cttattcttt cctttcattt ataaacattg
    28621 tagaataaag aaagtacaac gaagtattgt gaaaaaacgt aataaataac gaggaaaata
    28681 caagtaggag gtaagctgtg aaagaatttt tgaaaaattg ggggattttg atccttgtat
    28741 tagcggctat tttattagct agagtgtacg tgtttacacc agttactgta aacgggcatt
    28801 cgatggaccc tacattaagt gatggacaac gattgatttc atctaagatc tcaaactatg
    28861 aacgaatgga tattattact acaaaggaac cgggggatga agaacggatg attgttaaac
    28921 ggatcatcgg tatgcctgga gatacagtca agatggaaaa tgaccagttg accattaatg
    28981 gcaaaaagta tgatgaacct tatctggatg agttcaaaaa agaattttca gaagataagt
    29041 tacaaggaga atatgcttat agctcaggat tccaagcaca agcagaaagt tcatccacct
    29101 ttacgagtga ctttgaatat actgtgccaa aaggaaagta tcttgttcta ggcgataacc
    29161 gcttgatttc aaaagacagc cgaatgtttg ggttagtcga caaagatatg atccaaggaa
    29221 aagttgtttt ccgttattgg cctctttcag aaattaaaat catgtgatga aaaaataaaa
    29281 agaagctgtg acatgttttt tgtcacagct tctttttccg aaaattcctt cttatttctt
    29341 ggagttgctc actttttttg caatctcatt ttaagctttt tggctgatgg ataagaaaag
    29401 cgaagcgttt ttgttcataa atagcttttc cccacgatgg aaggtatgac tagtagcaga
    29461 gacaaagccc gttttttcca atcgattatt caattcttct tggctaaagc cattatggac
    29521 tttcggatga ctaatttgtt cattcttgtc aaagtctacg atgatcaatt gaccgccagg
    29581 agctaaaatt tcataaagtt ttgtaagaat attctccgta tcaggaatgt gtaaaagtac
    29641 aagtgataga aggatgagat tagcttggac agcaggaaat tcgacagaag catcagcgtg
    29701 gatcgttcgg acatttttca gatctgctgc ttggatcttt tcttctgcca ttttcagcat
    29761 agtttctgaa gcatcggcaa tgatcagttc tttgaatctt tccgcaagcg gtaaactaac
    29821 aagcccagtt ccgcctccgt aatccagcag gactttttgg tggctgtcgc gcggtagata
    29881 agtagttagt tcttcgcgaa taatcttggc taatgcttga cggtcagggg aatcataatg
    29941 gttagcgatg gaatcaaaaa tattttttgt catcaaatag ctccttacgt aaaactttga
    30001 aattttaact tattatagca cgtgaaaaga aagaaagcat gctatactca attcaaaaag
    30061 ggagaatcta gaaagaatat gccgatatcg tttgtaaaag atagagagga aaaaggaaag
    30121 tgtgtccgag aaatattact agacttgcct gagtggtttg ggcttcctga atcaacggaa
    30181 aaatatatcg aggaatcaag taaactacct ttatggtgtg aaaaaagaaa agaagaatat
    30241 ttaggcttta tcactctttc acagacaagt gaagatactg ccgaaatcta ttctattgta
    30301 tgggaataaa aaaagactat catcaccaag gattaggaac aaaacttttt aaagaagcag
    30361 aaggttacgc agcaaaacat tacaaatatt tgcaagtgaa gactgtagac gaggggcact
    30421 actctatata tgatcaaact atttgtttct acgaatcatt aggattttca aggttggaag
    30481 tttttccaaa tttatgggat gaatggaacc catgtttagt tcttgtcaaa aaattggaac
    30541 aaaagtgagc aatagattta atactaatct gtgcttgatg aaaattctca ctgtaaaaaa
    30601 tgctgaaaat caagtataat accataggaa caaactggaa ggtgaaaaag agcatggcac
    30661 aactattttt taagtatggc gcaatgaata gcggcaagac gatcgagatc ctaaaagtcg
    30721 cacataatta tgaagaacaa aataagccag ttgtcttaat gacaagcggt attgatacaa
    30781 gagatggttt aggcgttgtt tcaagccgaa tcggactgaa acgtaatgcg atgccgatat
    30841 ttgaagaaac agacgtattt gacgtaattc agaaaatgga aagcaaaccc tattgtgtgc
    30901 tgatcgatga agcgcaattc ttaaaaaaga aacatgtcat tgcttttacg agaattgtgg
    30961 acgaattgga tattccggtt atggcatttg gtttgaaaaa tgattttcga aacgagttat
    31021 ttgaaggatc aaaatattta ttgctttatg cagacaaaat cgaagaaatg aaaacaatct
    31081 gttggttctg ccacaaaaaa gcaatcatga atttacatta tattgatgga aaccctgttt
    31141 atgaaggcga tcaagtgcaa attggcggaa atgaagctta ttaccccgtt tgtcgaaaac
    31201 attactttca cccatcaatg attacagaag gagaataagc atgtacgatc aattacaagc
    31261 aattgaagac cgttatgaag agcttggaga acttttaagt gacccagaag tgatttctga
    31321 tacaaagcgt tttatgcagc tttcaaaaga agaagcaaac acgcgcgaaa cagtcgaagt
    31381 ttatcgtcgt tataaagaag tagtacaagg aatcaccgat acagaagaac tgcttggtga
    31441 aaaactggat gatgaaatgg ctgaactagc aaaagaagaa ttatctgaat taaaaaaaga
    31501 aaaagaagtc ttagaagaaa aaatcaagat cttgctttta ccaaaagatc caaatgacga
    31561 taaaaatatc atcatggaaa tccgtggagc agcaggcgga gacgaagcag cattattcgc
    31621 tggcgatctt ttcaatatgt accaaaagta tgctgaagca caaggctgga aaacagaagt
    31681 gatggaagct aacgttaccg gaatcggtgg atataaagaa gtgatcatga tgatcaccgg
    31741 agacaacgta tattcgaaat taaaatatga aagtggtgcg caccgcgtcc agcgtgttcc
    31801 ttcaactgaa tcgcaagggc gtatccatac ttccaccgca acagttgtcg tgatgccaga
    31861 agctgaagaa gtagagatcg atattgaaga caaagatatc cgtacagata tctatcatgc
    31921 ttcaggtgct ggcggacagc acgtcaataa aacagcatct gctgtacgtt tgactcactt
    31981 accaacaggg atcgttgttg ctatgcaaga tgaacgttct caaatcaaga accgtgaaaa
    32041 agcaatgaaa atcttgcgtg ctcgtgtgta cgataagatc cagcaagaag cacaaagcga
    32101 gtatgatgcc aatcgaaaat cagcagtcgg aactggggat cgttcagaac ggatccggac
    32161 gtataatttc ccgcaaaatc gtgtgactga tcatcgtatc ggattaacta tccaaaaatt
    32221 agatcagatt ttagcaggga aattagatga gatcgttgat gcattgatca tgtatgatca
    32281 aacatcaaaa ctggaagcta tgcaaaatgg gtaaaatgac ctatcgagaa gtcctgtcac
    32341 gggcttcttc ttttttagag gatcatggga aagaagcata tagtatccaa tttttatttt
    32401 tggaacgaaa acaatggaaa aaacttgatt ggctccttca tatgaacgaa gagatttcag
    32461 aagaagaaca gcggttgatc gaaaccgatc tgcgacttct gcttgcagat catccacctc
    32521 agtatttgct aggatatgca gatttttatg atcatcggtt aaaggtaact gaagcaacac
    32581 tgatccctcg accagaaaca gaggagttag tcgaatggtg tttagacgag acgcctggtg
    32641 taccgttaga agtgatagat attgggacag ggactggagc aattgctatt agtttgaaag
    32701 cagcaagaaa aaattggcat atatctgctg tggatctttc agaagaagca ttagaagtag
    32761 cgaaagaaaa tgctcagaaa gaaggaacga aaatcagttt ttatcatgga gatacattag
    32821 gaccagtaat ggatcagcaa tttgatgtga ttatttctaa tccgccgtac atcagtcgga
    32881 atgaatggga actgatggat gaaagtgtcc ggtcattcga acctaaaatg gccctttttg
    32941 ctgagaacga tggattagct atttatgaaa aaatagcagt tgaagcacca agcgtactaa
    33001 aatcaaatgg aaagattttt ttagaaatcg gatttcgcca aggtgaagca gtaaaaaaaa
    33061 tatttcagca agcatttcca gataaaaaag tggcgataaa aaaggatctt tttggcaatg
    33121 aacgaatgat ccaagtttat ggttgaagtt gtggatgaaa aattttttat ttgttttttt
    33181 attataaaaa aacacttatt tgacaaggat gtaaacgact ttttggttta tcaacaagtt
    33241 tatccactat tgacaaatag attatccaca aacagtggaa aacttgagga taaactcaga
    33301 caaaataaga atgtatgaaa aaggggttgt ggataaactg tggaaacgaa acgatatgat
    33361 gaaacagaat tgcaagaggc agccgaagca ctgaaagaag gagaacttgt tgcttttcct
    33421 accgaaacag tatacggatt gggcgccaac gctcttttac caaacgctgt caagaaagtg
    33481 ttttctgtaa aagggagacc gcaagataat ccattaatcg tccatgtcgc ctcttttgaa
    33541 caagtaaaag agtatgtgga caactttcat ccggcagcag agaagattgt gaagaacttc
    33601 tggcctggac cattaacttt gatatttaag atcaaaaaag acactttgcc ctcagttgtg
    33661 acgggaggat tatccacagc tgcatttcgt ataccggata acaaaaaaac actagaagta
    33721 atcgaacttt ccggtgttcc gcttgtcggt ccaagtgcaa atacgtcggg aaaaccaagt
    33781 ccaacgactg cagatcatgt atatcatgat ctacaaggaa aaattaccgg gattattgat
    33841 gatggagcta cgcgaattgg tgtagaatcc actgttcttg atttaagtga tccaacagcc
    33901 atgcctatga ttttacgacc aggtgcagta acgaaagaac aaatcgaagc agtcatcgaa
    33961 tctcctgttg ctattgatca gcatcttgta aaagaaaatg aaacaccaaa agcccctggg
    34021 atgaaataca aacactactc acccgataca cgtgttctga tggtcagaga tggcgattgg
    34081 tcaactgcag ttcaatgggc aaaaaataag aagatccgtg caggtgtcat agcaagcccc
    34141 gaaattgcgg atcaagtgcg gacagatact gcagctgttt atatgtataa cgacaactca
    34201 gtagaagcgg cggctaaagg attgtttgca ggtctaagag gattagatga accaacactt
    34261 ggattagatt tgatttttgt acaagtgtat ccagaaactg gattggggaa tgcttacatg
    34321 aatcgcttga aaaaagcagc aggacaaaat tattttgaaa aatgatcaat tggttattgt
    34381 cgcattcttt tagtatgata caatgctatc aagtaaatct tctatcagtg aacgaaaaac
    34441 taagcgtaac taatcgtaat agaagagata cgaatatttg gcaatttgtt tttgatggga
    34501 aaaataaaat tgtaggaaaa taaaattgta ggaaaataaa aaggagctgt tgtttgtggt
    34561 agattacaaa acgtttgacc ctgatctttg ggctgcgatc gccaaagaag aagaaagaca
    34621 agaacataat ttggagctga ttgcctctga aaactttgtc tccgaagcag tgatggctgc
    34681 acaaggaagt atcttgacaa ataaatacgc agaaggttat ccagggcatc gctattatgg
    34741 tgggtgtgaa tttgtcgata tcgttgaaaa tttagcgatt gaccgtgcaa aagaattatt
    34801 cggagcgaaa tttgcgaacg tccagcctca ctccggctct caagccaaca cggctgctta
    34861 tctcgctttg gtagaaccag gggacacgat cttaggaatg gatctttccg caggcggaca
    34921 tctaacacat ggatcaccag tcaacttcag tggcaaaacc tatcactttg ttgcttacgg
    34981 tgttgatccg acaacagaag ttattgatta taatgtggta cgtattttag ctagaaaaca
    35041 tcaaccaaaa ttgatcgtag caggtgccag tgcttacgga cgaacgatcg actttgcgaa
    35101 attcagagaa atcgcagatg aagtaggcgc aaaactgatg gtagatatgg cacatatcgc
    35161 aggattagta gcagcaggac ttcatcctaa tccagttcct tatgcagata tcacaacgac
    35221 taccactcat aaaacattgc gtggccctcg aggcggcatg attttgacaa acgatgaagc
    35281 attagcaaaa aaaataaata gtgcggtatt tcctggtatt caaggcggcc ctctagagca
    35341 tgtgattgcg ggtaaagcag tagcattcaa agaagcatta gatcctgcat ttaaagaata
    35401 tagtgaacaa atcatcgcca atgcaaaagc aatggtcaaa gttttcaatc aagcaattgg
    35461 aaccagagtc atttcagggg cgacagataa tcatctgatg ttgatcgatg ttcgcgagtt
    35521 aggtataaat ggaaaagaag cagagagcat tttggacagt gtgaatatca ctgtaaataa
    35581 aaactcgatt ccttttgaaa cactaagtcc attcaaaaca agcggtattc gaatcggcac
    35641 gccagcaatc actactcgcg ggttcaaaga agaagatgct gtaaaagtgg ctgaactagt
    35701 tgtcaaagca ttacaagcaa aagatgataa cgctcaacta gacgaagtca aaacaggtgt
    35761 acgcgaatta actgaaaaat ttccgttgca taaaaaataa aaatttaaat tccattagta
    35821 aacagaaaag ttcaatgttg ggcttttctg tttatttttt gggaaaacgg attctttgac
    35881 aatatcggtg atgctctcta gtatatgagg tgtaatttta gtcttcacac taaatctcag
    35941 ggaggaaaag tcatgactga tcgtatctcg ttagaaaagg aagcagtagc attcagtgat
    36001 gcaaatgcgc ctcatccgcg tatctatgaa ctagcaccgg aagaaggaag agttttacta
    36061 gaagaagtac aagaatcacc tgtgaacaaa tattccgtcg atattgaaga cttgatggtg
    36121 gatactggcg aatggggaaa actcaatatt cgtctcgtac gaccagaagg gaatatggat
    36181 aaattaccag ttatttttta tattcatgga gcaggctggg tttttggcag tgctcaaacc
    36241 catgataaat tgatcagaga acttgcggtt cgtacgaatt ccgtcgttgt ctttccagaa
    36301 tatagtcgat caccagaagc aaaatatcca acagcaattg aacaaaatta tgctgtgttg
    36361 caacagctga ataaattagc caagtcaaaa aatctggatc tcgagaatct aatcgttgca
    36421 ggagattcag taggtggtaa catggctaca gtcatgacga tcttaacaaa agaacgaaag
    36481 ggcctcccaa tatccaagca attgctgtat tatccagtca caaacgctga ttttgataca
    36541 gattcctatc atgaattcgg agaaaactat tttttgacaa gagaaggaat gatgtggttt
    36601 tgggaccaat atacgacgaa tgaagaagag cgtgctgaaa tcacagcttc gcctttgaga
    36661 gcaaatataa atgaactaaa tgatcttcct tcagcaatga ttttaacggg agaagcggat
    36721 gtattaagag atgaaggtga ggcttatgcg cgcaaacttc gtgatgcagg ggttcctgtt
    36781 acacaagtac gcttccaagg aatcatccat gattttgtca tggtaaatgc tatggatcag
    36841 acaaaggcaa cgcgtgcagc aatggcatta tctacttctt ggatcaagaa aaaagaataa
    36901 gctttgcttg tccgcaggaa aacttctttt caaaaacgga ttttcttagg aaaaatggcg
    36961 agtttgacta gtcaatcact tgattttgtt ttacaattaa aaagaaacga cagaacaaag
    37021 gagagcttaa aaaatgggca aatttcaagt gatcgatcat ccattgattc aacacaaact
    37081 aactattatt cgggacaaaa actgcggaac aaaggttttt cgtgaagtag tcgatgaaat
    37141 cgctatgttg atggcatatg aagtatcacg ggatatgcca ttggaagaca tcgtgatcga
    37201 aacaccgatc actgaaacaa cacaaaaaac gttgtctggt aaaaaagtag cgattattcc
    37261 aatcttgcgc gcaggtattg gaatggttga cggtatcttg gaattgattc cagctgctaa
    37321 agtgggacat atcggtcttt atcgagatga agaaacattg gaaccacatg aatatttcgt
    37381 caaacttcca gaagacattg atgcacgtca gttgtttgta gtagatccaa tgttggcaac
    37441 aggcggatca gcaatcatgg caatcgatgc attgaaagaa cgtggcgcgt cgaacattaa
    37501 atttgtttgt ttggtagcag cacctgaggg agtaaaagct ttgcaagaag cgcatcctga
    37561 tattgacatc tatacagctt ctctagatga aaaattgaat gaacatggtt atatcgttcc
    37621 tggactaggg gatgcgggtg accgtttgtt tggtacaaaa taagtaaata cgaaaaaggg
    37681 tgtgacacaa cttttgtcac atcctttttt ggtcattttt aacgtttgat gcagaacagc
    37741 tttttcacag ctttcgtttg catataaaaa aaccatccca aaaatgcaga gatggtcaaa
    37801 ttcaaatctt ttttagttct ttgtagttaa cgattactac acccttactt tgcgtcatca
    37861 agttcgcatt ttctgcagtt tcatcaatct caacaacagc ggaatttttc aattgctttg
    37921 aaatcgttcc agtttgcgac ttgccgtgaa ttgtgaacat tacgtgagta ttgatcttga
    37981 atttttcacc aatttccgat ggtacgatag ttgtatcaaa ttttccaaaa gttgccattt
    38041 tcataaaacc tgcctttcac atcaattata ccacgtttta tgaaaaatat catgaaatta
    38101 aactaatttt ccatcggact ctttacaatc agccaaatag agtatataat ttcttttgga
    38161 taaggccagc ttagctcagt tggtagagca acgcactcgt aacgcgtagg tcgtaggttc
    38221 gatccctata gctggcatta aaagagaaga cagatatatc aaggattttt aatccattga
    38281 tacatctgtc tttttgttta aaggaaataa agtatgcttt cttagcaata gagcatgtat
    38341 tatttctata aaaatttagg agaataaaac gactagcaat atagctgtga aatcaaatat
    38401 cacatgaact gcccaacttg taatgatcga gttgctttta atggcagctt ggttgaataa
    38461 caatcttgcc accccttgta tcaaaaggat gtgtgctaca gtgtgtaaaa tatttccatt
    38521 atcataagta ctgtaatgga taaatccaaa gatgattgct gacaagaaac tagcgacagg
    38581 aatgctgaac ttactagaaa aaatagcgag aaaatacact gagaacaatt cttctccaag
    38641 tatcatgata ggcaaaataa taaataataa cgggctgaaa gcctcattta ttggattgcc
    38701 tgttaaaccc catttcaaaa tttgttgcaa aaataccgag actgcggcac tgattactac
    38761 attgataaaa aaatatttgg ctatattttt gacaggagcg acaggtttcg agaacatttt
    38821 tttcgtatct tttttaccaa aaacaagtgt aaatgctata aatccagtga tagttaaaga
    38881 aaaaatgcta ataatggatg gcgcaaatag caaccctaac aaaccgagta caacgacaat
    38941 agtgcccaat atcccttttt taagttctat tttttttcct aaaatgttat tcatgataat
    39001 aactcctact attcagtttt tgaagataga gtcagtatac atgaagagtt
//
LOCUS       DAZBSA010000014        38596 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000014 DAZBSA010000000
VERSION     DAZBSA010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.014"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..646
                     /locus_tag="ACHGMT_000676"
     CDS             23..646
                     /locus_tag="ACHGMT_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834445.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /gene="trpX"
                     /locus_tag="ACHGMT_000677"
     CDS             1096..2145
                     /gene="trpX"
                     /locus_tag="ACHGMT_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN6834446.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="ACHGMT_000678"
     CDS             2142..3023
                     /locus_tag="ACHGMT_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6834447.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="ACHGMT_000679"
     CDS             3026..3796
                     /locus_tag="ACHGMT_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6834448.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="ACHGMT_000680"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="ACHGMT_000680"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFN6834449.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="ACHGMT_000681"
     CDS             5107..6270
                     /locus_tag="ACHGMT_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFN6834450.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="ACHGMT_000682"
     CDS             6387..6797
                     /locus_tag="ACHGMT_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN6834451.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="ACHGMT_000683"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="ACHGMT_000683"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFN6834452.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9370..9654
                     /locus_tag="ACHGMT_000684"
     CDS             9370..9654
                     /locus_tag="ACHGMT_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834453.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9814..10050
                     /locus_tag="ACHGMT_000685"
     CDS             9814..10050
                     /locus_tag="ACHGMT_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFN6834454.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="ACHGMT_000686"
     CDS             10047..10445
                     /locus_tag="ACHGMT_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFN6834455.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQTSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(11112..12056)
                     /locus_tag="ACHGMT_000687"
     CDS             complement(11112..12056)
                     /locus_tag="ACHGMT_000687"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFN6834456.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(12352..13296)
                     /locus_tag="ACHGMT_000688"
     CDS             complement(12352..13296)
                     /locus_tag="ACHGMT_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFN6834457.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLKIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            13517..14731
                     /locus_tag="ACHGMT_000689"
     CDS             13517..14731
                     /locus_tag="ACHGMT_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN6834458.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            14753..16768
                     /locus_tag="ACHGMT_000690"
     CDS             14753..16768
                     /locus_tag="ACHGMT_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFN6834459.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16851..18224)
                     /locus_tag="ACHGMT_000691"
     CDS             complement(16851..18224)
                     /locus_tag="ACHGMT_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFN6834460.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(18250..18534)
                     /locus_tag="ACHGMT_000692"
     CDS             complement(18250..18534)
                     /locus_tag="ACHGMT_000692"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN6834461.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(18536..20551)
                     /locus_tag="ACHGMT_000693"
     CDS             complement(18536..20551)
                     /locus_tag="ACHGMT_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN6834462.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(20730..21035)
                     /locus_tag="ACHGMT_000694"
     CDS             complement(20730..21035)
                     /locus_tag="ACHGMT_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFN6834463.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(21124..22155)
                     /locus_tag="ACHGMT_000695"
     CDS             complement(21124..22155)
                     /locus_tag="ACHGMT_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN6834464.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            22362..23237
                     /locus_tag="ACHGMT_000696"
     CDS             22362..23237
                     /locus_tag="ACHGMT_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN6834465.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(23389..24231)
                     /locus_tag="ACHGMT_000697"
     CDS             complement(23389..24231)
                     /locus_tag="ACHGMT_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFN6834466.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(24323..26200)
                     /locus_tag="ACHGMT_000698"
     CDS             complement(24323..26200)
                     /locus_tag="ACHGMT_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFN6834467.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(26197..27618)
                     /locus_tag="ACHGMT_000699"
     CDS             complement(26197..27618)
                     /locus_tag="ACHGMT_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFN6834468.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(27621..31010)
                     /locus_tag="ACHGMT_000700"
     CDS             complement(27621..31010)
                     /locus_tag="ACHGMT_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFN6834469.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            31267..32658
                     /locus_tag="ACHGMT_000701"
     CDS             31267..32658
                     /locus_tag="ACHGMT_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6834470.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(32731..33378)
                     /locus_tag="ACHGMT_000702"
     CDS             complement(32731..33378)
                     /locus_tag="ACHGMT_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN6834471.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(33386..34072)
                     /locus_tag="ACHGMT_000703"
     CDS             complement(33386..34072)
                     /locus_tag="ACHGMT_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6834472.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(34078..35151)
                     /locus_tag="ACHGMT_000704"
     CDS             complement(34078..35151)
                     /locus_tag="ACHGMT_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6834473.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            35373..36047
                     /locus_tag="ACHGMT_000705"
     CDS             35373..36047
                     /locus_tag="ACHGMT_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN6834474.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            36052..37392
                     /locus_tag="ACHGMT_000706"
     CDS             36052..37392
                     /locus_tag="ACHGMT_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFN6834475.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(37541..38527)
                     /locus_tag="ACHGMT_000707"
     CDS             complement(37541..38527)
                     /locus_tag="ACHGMT_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFN6834476.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT        11877 a         7044 c         7310 g        12365 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagactt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga aaatatggat
    10561 taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg aaatagtggt
    10621 ttagaactag tgggctgatt ttttatatat tttagtttac ataatatata ttatacaaag
    10681 tagatttaaa aaatgataaa cctctctcga acggtttttt atcaaaaatc gtcatttttc
    10741 catcaaaatc tgcttatgca aaattataaa aaaattttgc agtttttctt actttttttg
    10801 tcgtttaatt gaatgataaa aaccagtttc tttattcagt taatcaaata tgaacgaatt
    10861 agatatgaat cattgtattt gccgttgcgg aaggattttt taattaacca cagataaaca
    10921 ttgataacga ttcgcttatt gtaatcaaca gattaaattt catatgttca tatgtacgga
    10981 tacattttcg gaccgattaa ttttaaccat tctatttatt tgcttaccta ttagatatca
    11041 ataaaaaact gcttaacagc tatttcagct gttaagcagt tttaggattt ttagttcata
    11101 ttcattgttt cttataatac agtgttcaat acttctctta aagcacttgt tgatttgctg
    11161 aacattgctt tttcttcttc gttgattgat aattcaacaa cttcacgaac accattttcg
    11221 tcaacgattg atggcacacc tgtaaagata tctttttcgt cgtattcacc agttaaataa
    11281 gcagatacag gcaatacagc ttgttcgtta ttcaagatag cttttacgat acgtgcagta
    11341 ctcataccaa taccatagta agttgctttt ttacgatcaa tgatctcata agcagcatta
    11401 cggactttat cagcaatcac atctaattca tcttgggcaa tacgatggtc tttttctaca
    11461 atttcaaaca ctggtttacc accgacagtt gtatgtgacc atgcagcaac ttctgaatca
    11521 ccatgttcac ctaagatata gccgtggacg ctgcgtgggt caacttttaa tttcaatgcg
    11581 atttctttac ggaaacgagt tgtatccaat gttgttccag taccgataac acgtgatgtt
    11641 ggcagaccag attcgttcca agcgatatat gtcaaaatat ctactgggtt tgaagcaaca
    11701 acgataatac cgtcgaatcc tgatcccatg atttctttta cgatttggcg catgattgac
    11761 gcatttgttt taactaaatc taaacgagat tgaccaggtt tttggttgat accagctgtg
    11821 ataacaacga tatcagcatc tttacattct tcatagccac ctgaccatac agccacattt
    11881 tcatcgcccc aagccatacc atctaaaagg tccaatgctt ccccttctgc tttttcttga
    11941 ttcacatcga tcaatactaa ttcattagag attccttggt tgatcattgc ataagcgata
    12001 cttgttccaa caaaacctgt accgacgatt actactttac gacttgtttt tttcatgaat
    12061 aaacagctcc ttttcataac tatttgttac tttcatgaaa agtatagtct tgtttattca
    12121 ttttatcaac atgttaatta taatagttgt tattataatt tttgatggaa acgaattcct
    12181 gaatacgcat aacagataaa tgacaaatgt ttacacctat atcccctgat aatacaacca
    12241 aaagtttaga actgaaaata aatccaaaaa aattcgctgc aacgatataa taagacagca
    12301 atcgcaccta aaacaatttt ccttcatatg ctttagtgaa agattttctt cttaatgttt
    12361 atttctccat tcattgggag tacatgttga atacttcttg aataatttga ataaagaagt
    12421 cgtttgttta tatccgatac gattagcgat ttcttccaac gaaaaactcg tttcttgaat
    12481 cagttccaac gcaaagtcga attttttctt tagaaccagt tcttgaaagg aagcatcaaa
    12541 atacattttc aatttattac taagatagac cggattgtag ccaaaatgag ctgctgtttc
    12601 ttttaacgta caagaagtaa aatgcttatc gatataatct aatactctaa gcatctcatt
    12661 ttctgcgcat ccatctgctg atgaaaagct ttgttgttct aataaatgag tcaattcgat
    12721 cagtaatagt gatgtataga gatttaacga acggatctta tgactgtatg tatcaaaata
    12781 aatcaacagc atattttcaa tcaaatgcgt caaacgactt gtttttccgc cgcgaaaatg
    12841 aatgaattgt gtatgttccc ctttttcttt tgatgcattt atcaaaaaag aagaaagtag
    12901 attatttgtg cttgccaaat gattcaatat ctctgtagat aaggtttctt cttttaataa
    12961 gatgttgatt aaaatatcct cttgccctgt gtattcgatt ctttgagtag cttctttatc
    13021 aatgatcacc acgtcacttt cttccatctc tatcctttct ccattaataa tttttaaaca
    13081 tgtaccgcta tacatataat tgatttcaac aaaatcatgt gtatgctcgg gaacttccgc
    13141 atatcgacgg tgtttactga tatgaatatt tccctgttga aatagatccc ctcggtggat
    13201 ttttggcggt gtctgaagat ggtaagctaa gtttaaatca ttgatattca ttccttgttc
    13261 acgctgcaga gtttcgattg attcttcgtt acgcaaaaaa aatgtctaat tcagctagtt
    13321 ccaattgact ccctcctttt tgtaagcggt ttctttcgct tattagttca cgctgtactg
    13381 ctattatatg caaagctatt tttggtttca taaagacttt ctatggttgg gacatgaact
    13441 tcctgtgatt ccgctattga gagaataacc acttcatact atatttttga taagcgctta
    13501 caaaaatgaa aggaacatgc ttatgaataa aaagatgatg aaattttctc tgctgtcagt
    13561 ctctcttatt ttgacttctg ctggtgcagt cacgtctgca attcccagta tgtcagctac
    13621 ttatgccacg cgttctttat ctgctgttga attattaacg actgctccct cttttatggt
    13681 cactattttt gttttgatta gtagttttat tgctaggaaa atcggacaaa aacaaactgt
    13741 gatcatcgga ttagtgattg catcgatttg tggtatcctc cccctcttct cacaaagtta
    13801 cagtattgtc ttagcttcaa gagctggact aggtattggc tttggattga tcaattcttt
    13861 ggcagtcagc atgattgctg ccttttatca cggagatgaa cgagcctcta tgattggttt
    13921 tcaaagtgca tttcaaggat ttggtgcagc tttaatgaca tttgttgcag gacagctagt
    13981 acagttcggt tggcaatata cctttttagt ctacgccatc agcttaccta ttcttgtctt
    14041 gtttgttttg tttgttccta cacctgaaac aactgaactt caatcgcatg gcacagcccc
    14101 aacaagacaa gtaatcaaaa cgaatcctaa aatgattagt tatggtattt ttctcttgct
    14161 ggtcatcgtg gtatataatg ctgtatcgat taaattagcc acagtcctga cgacttattc
    14221 tctaggaaac gaaactaatg cagcgaatat cttaacaatc atgcaattgg caagtatggc
    14281 tgccggagtt tgttttggct ggcttcagac aaaagcacat cgctatatgc ttacactttc
    14341 tttactactg atgggaatcg gctttgtatt gattgccact gtgataaacc tgtatgtgat
    14401 tgggtttggt gcgatcctaa caggaatttc tttttcgtta ttcattcctt acttattcaa
    14461 ccaagtaagt atccaagcgc ccaagtacgc ggagagtttc aatacttcaa tcttattggt
    14521 tggcgctaat ttaggtactt tcattgcacc atatggtttg ctctttctct cttttgcttc
    14581 tcttgggagt aaaacacttg ctgttttcgt caatggcagt atcttgctgt tgattagcgc
    14641 gcttattagc cttctagtag tacacaggac gaatagaaag acagtgataa gagtagatga
    14701 agaaataaaa gaacaattga caacaaaata aatttgatgg aggtcgtgta aaatgacaaa
    14761 tgaaaaacat aaagatagat ggctttggta tcctggagac tttgagatcc gccatggact
    14821 gctacaaaat ttccaacggg aagaacgagg ttttgactgg ccagcgtact ggtacatgga
    14881 tgattgccat cggaacgtca agtttaagag atattacttt cttgatcaac cttccatgtt
    14941 taaagtgacg atccaaggag ttgggtatgt agaaattaat ggacaaaaac atccatgtgg
    15001 aaaatggctg acttgtccag ctggtaaagc aaaaattcgt atctttgtag gacatacatc
    15061 agggctacca gcgatgttta tcgagggaga cgaagtaaaa tcagatattg gctggacagc
    15121 aagtaatttt attgaagaat atccagctgg ctggagtccg ctttatgtcg atatcgcaaa
    15181 agatccaaat caaatctatt atcaaaaaga atgcgtccat ccagtcactg aaacggagat
    15241 tggcggagga atcttattcg attttgggcg agcagtgaat ggctgtgtag aactaatatc
    15301 tctggatgac catatcgaat ctatcactct atgttatgga gagtccgatg tagaagctat
    15361 ggatatcgat tattgctact ataagcaaat aggaatgaca agaaaaatga aagctcgtaa
    15421 acgcgctttt cgctatttgt ttataccaga ggtttctact ggaatggttc aacttcaagt
    15481 ttttcatgaa tttttatctc tttcgaaaca agctgctttt tcaagcgatt cttctttact
    15541 gaatcagatc tggtcagtat cagaagaagc cttcaagtta tgtagtgatt tgttttttat
    15601 cgacggtatc aaacgagatc gctggatttg gtctggagat gcgtatcaat cattcatgat
    15661 caatcaatat cttttttttg acgaggaaat caataaacga acgatattag cattaagagg
    15721 acaaaatgaa atcaagcagc atatgaatac tatcgttgac tattcgatct tgtggttgat
    15781 tggtatcgaa aaccactata tgatgacgaa agactatgaa tttttacgca tcgtctaccc
    15841 taagatgcaa tcattaatga gatacttgat ggagcaaacg aatgaattag ggtttattta
    15901 tgggagagca aaagattgga tatttattga ttggtctgaa atggataaag aaggaacgct
    15961 tgctgcagaa caagtcttat tactgaaagc ctatcattcc atgatcactt gcggagatat
    16021 cttagctgaa acatatggag agaacgaaaa ttatcatact catctctatc aagaaaaata
    16081 tcaactattg aaacaaaacc tatttgacta cttttgggat gaagaacttg gcgcttttat
    16141 cgattgctat gaatctggga gaagaaatgt gacgcgtcat gcgaatattt tcgctgtttt
    16201 attcgatttt gctaacaaag accaacagca atcgatctta aagaatgtct tattgaatga
    16261 cactatcaca cagattacta ctccatattt caaattctat gaacaagatg ctttatgcaa
    16321 actcggacag acgaatatcg tctaccaaac gattcttgat tattggggag ggatgttatc
    16381 tcatgatgcg gtaactttct gggaagaata cgaccctact caaacaggaa atgaaaagta
    16441 cagtatgtat ggagatcctt atggaaaaag cttatgccat gcatggggag caagcccaat
    16501 ctatctttta ggacgctatt ttatcggttt gtatccaaca aagccaggtt atgaagaatt
    16561 tatgatacaa cctaatctag attcatttga aacactgaat tgtacgctac ctatcaaaga
    16621 tggaaagatt tcgcttgaaa aaacaaaaga taagatcgtg attacttctg atagaacagg
    16681 cggaacagtg aaaatgaatg gtaaaagtta tcctcttcta gcaaaacagc cacttgtctt
    16741 aacaagctat tcaaaaataa atgcataaaa caaaaaactg cttgcgacat atcagaagac
    16801 tttaaatctt cttctgatat tgtccaagca atttttattt tgaagtttgc ttaattctgt
    16861 tctttagaaa caatgtttgt tctttttccg gcaaaaagaa agctagataa tacgacaagt
    16921 aaaaagaaag cgatgattcc aactgtcaag gctatttgac cgtattctcc tagtttccca
    16981 agtacaagtc ctggtactag ataatcagta tcggcaaatg tagaagaagc gattttaaga
    17041 tctcctaata ctggaaggat aaataatggc agccaactaa tcaataagct attgaaagca
    17101 gaacctaata ccgcccctct tctaccgcca gtcgagttgc cgaagacacc agaagcagct
    17161 ccgcagaaga aatgtcctac taccccagga atgataactg tagtttgcaa gcctatcata
    17221 gcaaacatgg atagtgttcc tacaaagaag ctaacaaaaa agccaattaa tacagcatta
    17281 ggtgcatatg gaaaaacgat aggaacatct aatgctggtt tagaatcggg tacgagtttt
    17341 ttagcaatcc cttggaatgc tggaacgatt tctgccaaca ccattcgaac cccttgaagg
    17401 acgataacaa acccagctgt gaatgttcca gcttggatca gtgcaaagat tacagcgttg
    17461 tctccattgc ttagttctct ttcaacaaat ccaggaccag ctaacaaagc aagtgcgaca
    17521 tatacgatcg acatcaagag catgatcgat acagtagaat ctcgcaaaaa gctgaaactt
    17581 ttaggaatct ttatgtcctc tgtagaacgg tctttgtttc caaaaaactt tccgatccat
    17641 ccactagaag cataaccgat atttccggta tgccctaacg caacttgatt gtttccagtg
    17701 atgttcttca taaacggttg acaaagagca ggcgtaatag ttaatagtgt tccttcgaag
    17761 accccgccta ataagatcat ccacaaattt gcagtaaacc ccgcgcttac taaaatgact
    17821 gctgttagac aagaaacata aagcattgct tgacctgtaa gaaaaatgaa tttatatctt
    17881 gtgaatctag caagtaaaat attcacgatc atccctacta gcatgatcaa agcagtaggt
    17941 gtaccgtatt gttgcaaagc aatcgccact actgcttcat tgctaggaac tactccttga
    18001 gtatgcagag ctgtttggaa catcgaagca aaaggggtta gtgaattact gattaaacca
    18061 gcaccgccga tcagtactag gaatccgaca aatgttttga tagttccttg gatcacttct
    18121 gaaaatttct tcttctgaag aacgagacca agcagtgcaa tcaaagaaac gaggatggag
    18181 ggctgactga cgattgacac aaacaaattc aacaatttat ccattgatag ttcctccctt
    18241 gaatcatttt tattcttttg acaagatctt cgttactttt tcttttactt cttcgctgtc
    18301 aatgatcgat tgcaaaggta cgagcttttc atcaggtata gaagttagcg tatctttcaa
    18361 agaatgctca atgaagaaat aatcagccat atcgctagat acacttccaa catccgtatg
    18421 acttacttca aactcgtcca catctgctcc aagatctgca agtgcttgtt tgatgttcat
    18481 ctctaccata aaacttgttc ctaatccact ttgacatact gcaaaaattt tcatatcatt
    18541 cgtctccttt tcttatgatc gctaatattt cttcttttgt tgatgcgtgt aataaatctt
    18601 gaagattttg tttattagaa agtagctttg ttaaatttgc caaagcccga agatgtgcct
    18661 cattatcgat agcagctaaa cagatgaaca aactaatggg atgggctgga ttatcaagca
    18721 gatccacagg ttgttgaagt ttcagcaaag acatccctac ttcgtttacg ccatcttctg
    18781 gacgagcatg aggtaatgcg atgtgatcgc caatatggat aaacgcacca tactgctcca
    18841 cgcgctcgat catagctttg atataacttt cttctatttt attttctgat aatagcggct
    18901 gtgcggcaaa tgtgattgct tcttgccagt tttttggttg atccgataat tggatcattt
    18961 cttctgttaa taattcagat agcataggac gttgatcctc cttcttttct aaaagtttgt
    19021 tcattttacg atttaatact tgatagagct tttcttcgtc tacgccttct tttaattcga
    19081 tataaggcag tagcgccgac aacaattctt gggcagaagg cattgaaaat cctggtatca
    19141 gccaatctct ttgtacttga ttgatcagct gatttttttc caagtcagtc atgattggat
    19201 gcagaacata gagagacttt ttcttatcag taagtgtgag aggtacagta gaaaaaataa
    19261 tatcgtaaga agtttcgtca acctcgtgta agcggtcagc tgaattagtc tctgtaaaaa
    19321 agatcatcgg gaataattgc tgaagttcac tttttaagat caatgaagag ctaatgccac
    19381 ttggacagac gatcacggct cttatttttc tatttctttc ttctgcgtcc gcttttctga
    19441 tctctccgcc aaatagtata gtgaaaaaac caatctcttc ttcagggatg ctcttcccta
    19501 ttcgtctttc taacggaagt aatgcttttc ttgtcatttc gtagatggaa gcatattgat
    19561 gcttaatttg atcgatcatc acattaggta aataaaaaga ataggaaata cgaaaatagg
    19621 cagggaccaa gtgataaaag acattcatca ataattcacg aaacgaagca aattctacgg
    19681 ccatcaggaa ttccatgcga tggatgactt ctgaagaaca ttccagcaaa aaatcaagtg
    19741 caggctcctg gacatctcct tcaacgatcg atgctaagca aagagagaaa aatataatct
    19801 ccgaattttg atgttcggaa tagccaaggc cagtcaatac ttgtcgacta atgtcagtga
    19861 ctgcaagaga agccaacagt tcagcatctt ttaccagttc catggatgtt tttccaattc
    19921 gaatggttgt caaactcaaa taataagcta attcatccat tcgatttggt gcgaatcgaa
    19981 gatctgatag tgaaatcgtt ttatctagca aatctctaac tttggcgtaa taatcaatgg
    20041 aaagggttgc ggttaaatac gtgagcccaa atcttccagc aagtgactgc atcaaacgag
    20101 cagtgcgatt ttttgcttct cttcttaata ttgcttctga ccctagcagc ctaaaaccag
    20161 ttttcctagt atacgacagc tgacaatctt ccacttctaa ttcatttctt atctttttga
    20221 tatctgacag gactgtccct ttagaaacct gaagaaaatc ttgaaaatga aaaacagaaa
    20281 cagacgttgc agtcgaataa accatcaaat aaagaagatc tcttcgctct tgttcggtat
    20341 acacagcttc aaatcgagta atatccaaaa atttttctgc ccagtcgatt gctcggaatt
    20401 tctttatacg gatacgggta tcatccacat attcggcaaa aggagttaat tgtttgatcg
    20461 cctcgcttgt atttacttcg atgatctctg ctgctttttc tagactgatt tctcttggtg
    20521 ttttcaggag ataactaatt ttgtagttca cagcgctgtt cacctcctac ttatattcta
    20581 gtatggtagc gcttttaaat cgaggggtgt ttggtacaac ttacagttgg atttttttta
    20641 cgactgtacc gaacgatttc tatcgaatgg atacagaaaa aaagcaggtg accaaatgtc
    20701 tcctgctttt atgaattgat ttcgtccgat caatgatgat catgtttatt ttttgaggtc
    20761 agttcttcaa atttttcttt gacatcttca accgcttttt cgatatcttt ttcaaagtga
    20821 ttgggctcat taggctttgt ttcctcttct acagaatttg aaccagataa agctgtttct
    20881 ttattttctg tttcaccaga gtttgtgcca gctaatgatt cttctttgtc ttctgtttca
    20941 ccagaattag ttcctgctaa agccacttct tcttctgttt taggggccat tgaacctctt
    21001 aatcgtttgt gtttttcacc gtcataattt gtcatgttgg ttcctcctag tagttctata
    21061 ctttttcatc gtacaataag acagtttatg cgtcaaaaaa caaacccgat tcttacaaat
    21121 atgtcatatt tttccttctg attcttgaat gcttttgatt ccttcttcca aaattttcat
    21181 ttgtaagatt gtttcttcat ctttgatgac tttttctgcg ccattaacta atgtctcgta
    21241 aatcccttcg tagacacggc tgtaatcacc aacctcggaa attacttttt cttcatgata
    21301 actcccttgt tcatctatat aggtcaatac accataatgt tcgggcaaat cgataccaaa
    21361 gtcttcatga tcaggcatgt aaaacatttt cagatgttct tcttgtcgat cttttgtttg
    21421 tttcacgaac atcccttttt ttccgtaaac gacgaaactt ggacgttctt tgatgcggaa
    21481 ataactggat tttatcgaaa ttttcatatt tccatagaaa aaatccagat cgaagtaatc
    21541 attcattcgt cctttcccta aaagttgacg tacatcatag ctgatatcat ctggttcgcc
    21601 gaaataagaa agaacttgat ctactgtatg acaagcatgt ccatataaat agctgttcgc
    21661 agtagaaaat tcatgcgtat tttctggtac ttccggtctg aaataatcat aatgcatttc
    21721 tacttctaaa atatccccta attttccgct ttcgattact ttttggactg tcaagaagtc
    21781 agaatcgaac cgtctgtttt ggtaacattg gataaataga cctttttctt ttgctaatgc
    21841 aaataactct tctgcttcct ttgacgtttc tgcaaaaggt ttctctacta gtacattctt
    21901 accatgttcg agtgccattt ttccatattc ataatgaaat tgactaggtg tagtgatgac
    21961 aactaattgg atctcaggat catcccaaag atcagacagc tgatcagtat attcgatgtc
    22021 ttcgaatgtt ttccacactg gttcagatgg ggaaaaaatt tttttgatat tgattttgtt
    22081 cgtaagtttt cttgaaaaag gcaaatgata gcgatttgta ctctttccat tgccaagata
    22141 agcgattgta agcatgagtg gtgcctcctt ttattgatgt tttcagtata tcctagaaaa
    22201 gcgcagaaag tatttataat agctattctg gtaaaaaagt tcagaaaaac acttgttatc
    22261 attcggtttg gtgaaattaa tgttcaaagt aaggataaaa atcaaaaaga tagtagcgga
    22321 accaagtgtt tatcagtaag atagaatttg gaggtcagat catgcttatt gaagaaaaac
    22381 taacaaaaca agagttgttt accacgacag agaaacggat tgtcgattac atccgtagga
    22441 atatcgaagc tgcagtctac atgacgattg aagaattagc aaaagctacg tatacttccc
    22501 attcagctat cattcgttta tgcaaaaaaa tgggattcag cggatttaaa gaattccgtt
    22561 tcgagctggg gcgggaagtt catcaattga ttgctcagtt caatcaaacg attgatccta
    22621 attttccttt ttcaagtgaa gatagcactc agcaaattgc aaagaaaatg gcggatttat
    22681 cgatccagtc aataaaaaaa gcacaattgc agattgaaca tgaagattta gatcaaatcg
    22741 gtaaagtatt gaacaaggca cagcgaatct ttctttttgc aaaaggtgat tctcaaatca
    22801 cggcaaggaa attccagaat aaaatggtca agttgaataa gtttttgatc atggctgaag
    22861 aatacagtga ttcttcttgg aatgcagcca atttgactag cgaggattgc gccattttta
    22921 ttagctacag cggtcgcatc catcattatg aacgaataat gacctatttg aagcatgtag
    22981 aggcccctac cttgctgatt accggaaatc agcattcaga aatggccaaa caggctgata
    23041 tgtgtcttgt tatatcgcag gaggaatatg attttgctaa agtagcgact ttctcctctc
    23101 aaattgcctt cgattacgta ttaaatacgt tattctcagt gatatattct caaaattttg
    23161 aagagaatat ccgcaacttg aaaaacaaac aagaattgat gcaaaaaggc cttcttaaag
    23221 aatatcggaa aacttaagaa aagatcctca tttagtaata agaaagatag tgtttgatag
    23281 gctctctttg atatcatcat aaaaacaggc tgaagttttc tttttcaaac ctcagcctgt
    23341 ttgtttacgg aacgattcga atagatatat tatagaaatg aggaatagct aacttctgtt
    23401 tcttattttg aacgattgac gaaaaagata tataagaagg aggaaaatca gtatacatgc
    23461 agctaacaat ccatacagga aaaatcgttt gttctcggat actttctgta cttctttttg
    23521 aacttctttt gcatgataag gaacccggtg ccctctgaca agtaagcgat gactgttgac
    23581 catataaggt gtacaagtta ggagtgtcac gaaatcctgc cctttttcga ttctcagatc
    23641 ttttgtatca gtcggttcta ccgtcttgat ctgatcgact tgataagcga gtgtccgtcc
    23701 attgatttcg atatagaaag aatcacctaa agtcagtttc ggtaagtctg tgaacaactt
    23761 tgcttgaggc agtcctcgat ggctggaaat aacggcatgt gtattttctc cccccgtagg
    23821 ataagacgtt ccttccaata atgaggcacc tttttctagc agcacagtat tcgtttcatc
    23881 aaaaattggc aaatggacat tgatctcagg gatcgtcaaa ataccgatcg tatgtttctc
    23941 aaaataggtt ctatcctttt tcgcaggttc ttttttctta gtaaagggat ctgcacctgg
    24001 atttccacct tcttttgcta attgctggtt tttctttgtc atgttttctt ggattttagc
    24061 catgacttct tcattttctt tgaccgcttg ttgctgataa tgagagatga tctgctgatc
    24121 cagataatta ttcagcgcgt cgctaacaaa tggataagct agtatcccta ttcctgagag
    24181 aagcaagaaa agcatcagca gatctagaag tttccttttc tttggtttca tttgtgttct
    24241 ccttgtgatg actgtttgat aataaaagaa aaaggagcat ttgcgtaatc cgctgctcct
    24301 ttttccaatt cttgcaaaaa gactaaatct gactgtgctt gcgtctagca aagtacagtc
    24361 cagcaatcag tagaaggact actcctgcac cgatatacac atagattccc ttaccgcctg
    24421 ttgaaggaag tgtacctttg tgtttatttg gtattttttc aggagaaatc aactgtcctg
    24481 ctgttacata agattgttca tcgatagtaa atgctacacg gtttgttaat ggaacatatt
    24541 tttctggcgc tttcgtttct tccagatagt acgtgccata ttttagacct gtcacatcga
    24601 ttaaaccgtt acttgtggtt gtaaaaaccg ttgctgattc tttcgccgat acccagctga
    24661 cggcttttgt ggatgggtcg atcgataaat atttcgctgt gtcactatct tgatcacgaa
    24721 cgacgaattc tgctccagca agtgtttggt ctgatgtaac gtcaccatct actttaacaa
    24781 atcgtttgcc cccagtaacg acatcgactg acggtggtgt ttgatcattt gtatgcccgt
    24841 tatcgacatt tgcttggtta gaaaatcctt tggttggatc tgctttttcg ttcaaatgca
    24901 tatagtaaac gaatttcaat gtaccgccag gagttaatga aggaatataa ttcggatcaa
    24961 ctgaaaccgt gaatccgtct gtttgctcag tgacagaata atttactggg tcgatttctt
    25021 tatttccatc atataaggca taagcatacg ttcctgaaga atcattatca aatgttaaag
    25081 cagcgtcatg agtatcgata agtttgaatg ttgtgtactt gttttgcgtt ccttctttat
    25141 cagcgatacc taatgggata ttgacagaaa tctcatattg aattttttca ccgattgcga
    25201 catcttttcc attcaattga ggtgttgttt tatcaacttt agacgtatca tttttgatgg
    25261 tcttttctac tggtgtttgg ctatttgcca ggatctcaaa aggcgtgatt gtttgctttt
    25321 caatcatttc cgcattatca ggagctttta cttcttctaa attatatttt ccaacttcta
    25381 aatgattaac gatcaactgg cctttttcaa tagatgcctc ggcaaagtca ttgttgccga
    25441 tgtcataaga atgaccagta atgaaatgtt tggctttggt ttgatcagtt gtccaagtgt
    25501 acaatccatc tgtgacactt tggatgtatt ttttgacgct tggtgttcct tcttctttag
    25561 aaataataaa ttctgctcca tttagtgctt cgttttcggc agtaccgatt tttgtaactt
    25621 tcaacgttcc atcattaccg actgtatttt tagggtagag atggatagta tctagttctt
    25681 ctgtcccgta tttataagag ccatctgctt gtttgatcat ctcatataca gggaaagcta
    25741 aaaccatgtt tgcggcagct gacactccgt cttttggttc ttctttgatc gtgtagactg
    25801 catctttccc attttgtttt ttaggtaaag ataaagtgac atttccatca gcatctgtcg
    25861 ttcctgaagc aactggtgta ccaggagtca aagactggac tgcttgtttt gctgcatcca
    25921 cggacgctcc tttatctcgt tgcgcataaa attcttgagt gacgttataa actgaaaatg
    25981 aaatatcggc taatccttgg tattgatcga attcgctcat ttctttcccg ctgttttgga
    26041 ttaaaggatc gggtaaatca gtcattttct ttttatgaag gatgacttga gctgctgtct
    26101 cctctgcaaa cacttttttt gcgccgaagc tgtttgtgag taatggtaaa ataaggaaaa
    26161 ggactcctaa cataacgttt atttttttat gatttttcat tttttcactc ctgttctttt
    26221 tttgataaac aaccagattg caaagcttgc gaataccata ccaacaacgc ctaaaagtgt
    26281 atttttcgtt tcgtttgttt ttggaagaaa tggacggcta ttttcttttt tattgactac
    26341 tttcaatata ctgtccgcct ctttatcctc agaaagaaaa gttgaaatgg taaacggaat
    26401 ttctgtttca cttaaaatat aaccagaagg tgcttcgatc tcttttaatc gataacttcc
    26461 tgttttcaac ccggaaattg aaaaagcgcc atttttatcg gaaatcaggt gaagcgcatc
    26521 tgattcgttc tttgtccact tgtatccgtt ggctgtttgc tggaggtaat ttcctgcttt
    26581 gtttttaata aggaatactg cgccagctaa tcgtttatct ggtttatttg tctcgatttt
    26641 gacaaattgc ttactccctg tatatacaga gactttcttt gtactgacac caaaaccaaa
    26701 atccagtgtt ccgttgttgt tgatttcctt gttcgcctgt gctgtactgc ttagctgcat
    26761 ctgataagaa atggtcagtt tttttccagc atatttttcc aacgaaggga tcgaaaataa
    26821 aacatcaaat ccgtgaggtt tcttgtgtat gacaaagcct tctgtcagct tttctccatt
    26881 cgatgaaatc gttaaactat taggtaaaaa cgttaatgct tcatccgcac tatctgacaa
    26941 ttcgaacttt tgatagtcaa ggatatttac aggaattgtc gtatgcaagg aataagtgat
    27001 tttttcacca atcgtaaaat catttctagg ctcttctaat accttctcaa aaggtggatc
    27061 agggtagtcg ttttcttcat ttttaggata caaatggata gttgaaagtt tttgattgtt
    27121 ttgtccatgt acaggaagaa caactaccat attctctgcc ttttctttga cgacttctgg
    27181 tactttggat tcaatgaaaa gatacgcttt atctcttttt tcttgatctt tagcggccag
    27241 agaaaaagaa gcgataccat cttcgttgtt aagagttgta gttgttgctt ctgcggtaaa
    27301 cataccggaa gacgcaccgt tttttgcgat ctctgcttgt gcttcttcta ccgttttgcc
    27361 cttttcccgt agatggtaaa aagaatctgt gacatcataa acttggaatg tgacaccatt
    27421 taatcctcga tacgtttgta ataaagcttt ttcttgtccg tcatttggat gatttttcgg
    27481 cagttgcccg tttgggaaaa gcaatttgtg taaagtaatc tgaaccattt ctgtttctgt
    27541 ttctgttgct acctgagtaa aagctggttt aaatagtaac aagaagagac acatactaag
    27601 ccaaccaagt tttttcatat ttagctgccc cctctttctt ttttcaaaaa aagataccaa
    27661 atagaaaacg cacaaccaat catccctact agatagattc ctaaacggcc aattcctccc
    27721 gtttcaggta atggtacttt tgcctgattc gtgatgtcta aagaaatctg gttgtttttg
    27781 gctcctggtg acagaacaga ttcatgatct tgtccatcca cttgaattga cccatcttcg
    27841 tgtataacta tagtaactgg ctcttttaga ccttggtatc cttctggtgt aaaagtttcg
    27901 gtcagcgtat aagttccagg ttttaaattt tcgaatagaa aggtatctgt ttcttgtcca
    27961 tcttttggcg attctaagtc catttctggt ccctgcagtc tgaattttgc tccttttaat
    28021 gtctgtccgt tgtcttcttt tttgtgtacc gttagatcaa acgcttttag tcgattttga
    28081 tgagtgagcg tccatacatt atttgtcatc tcggtatttt ttcccgtgta aatgattttt
    28141 ccatatttat cgatttggaa ttcataattt cccggcgaag tatcatatcc ggcaggacca
    28201 gctgtttcaa cgactcgata tttaccaggt gtgagtgaat caaaactggc aagacctttt
    28261 tcattcgttg tttggctgtc aattggctga taagtaccat ttgcttcttt tttctgtagg
    28321 gaaaatgctg ctcctttaag atttatctct ttgtccgtcc cttgcatcgc atacttacgt
    28381 actgctacag gaacttcaac aaaaggattt tcaatagtca actggatcgt atcatcggaa
    28441 gtagtgactg tttttccatc aatggttacc gtaccatcag aagcgatctt gatttcccaa
    28501 gtagtctttt tgcttaatcc atgcccttct ggagcttttg tttctgtcag cgtataggtc
    28561 atttcttttt gcaatttgac attttctgga agagaatagg ttccgtcgcc atgatctgtt
    28621 agtaaagtat caatagaatc tcctgttaat ttgaaaacag cgccaataag atccttttca
    28681 cctgtagagg aatttttcgt tattttcaag tttaacggtg tgaattgttt agtatttttc
    28741 catgtcattg catctatttg ttgagaatcg tatcctggta caggtaattc tttgattgtt
    28801 tggtaactga atgcttgacc ttgattattg tattgaggta gtgataatat ctcttgataa
    28861 ctttctccgc tatttgcaga taatttgtca atgtctgcac gttcccacgt atttgttgta
    28921 tctttagctg gtttaatgat tcgaatatat ccgtttttcc aagctgcagc atttgtcgta
    28981 tgttcccgtt gaatctcaaa agtaacttga tctggacgat cagctagatt gttgtcaaat
    29041 tcttcccata actttttgat gtgaagactg actccgggag ctttagcaga tggtataccg
    29101 aattcagcta attcattggt atcaatgttt ggctggaaag ttgtcctgcc gttcatttga
    29161 taccagaaat ttggatggaa gtcctcattt tctgtttgga ttctcacttg gtaatggatt
    29221 tggatttctt ggtcttttcc taaatagatc tgattgctct tgatggtatt tccttctatg
    29281 gaaatagatg gagtgaccgt tgtaggactt gtccccacac tcttgactga aagtgtacca
    29341 ggctgataaa tgaatggttc tgcgattgga tcatttattt gtccattgct tacagttgct
    29401 gagatctgta cagctttttt ggctaggtat tcagagatat ctgttgcatg atcagctgat
    29461 tcatagtaaa gatcgccttt ttcatctgat gaaaccattt gtcgcatacg agactctact
    29521 tctgcttttg agagaccagc tgccggatcg ctttgaagtt ggatgccaag accatgtatt
    29581 tcgattcctc gttctttgag tgccatcgct tctccgatgg ttgcgataaa cgtactgtcg
    29641 attcttctgg atagattgtt ttggtcaggt gcatcataga ttcttgagat tagagaagta
    29701 tttcccggcc gatcttgcgt attagaaaac tgagttccgt aataattgct gcttgattgt
    29761 gcgtgtaccc gctgtacttt ataggaaaat gttggtacac catccgtcag caaaacgatc
    29821 acttttttat gtccatttgg aacggatagc atgcttcctg catctcttag tgctttttgt
    29881 gtaaaagtac caccatttgt ccgtgaaggt gtaatggatt ttacttgatt tttcactgaa
    29941 tcaaatgaac ccatctgtac tgcaccgtta ctgtagctat atccttcgct tgagtagccg
    30001 acatatccca tattgatttt gtctgtgata ccgctatctg ctaaagtatc gacaaaacga
    30061 tcgacaccaa tctttacttc accgatccga ttattgtcgt tcatacttcc tgaccagtct
    30121 acgaccaata cgagatcaag aggcgtgata tctttttgta catttccacg agcattcaaa
    30181 taaacatcaa acaatccggg tgtacttgtt tcctttgcat actttcgtat cgcatagtct
    30241 gcttgattac cggttcctcc atattcgata tacgaatggg tccgatcatt aggatctcca
    30301 ttccaagaat ttataccatc ccattgattt gttccgtttt tattgccttg atgatcaaga
    30361 acatttgtat ttcctgtagg ttgccaactg gctttaggat aagtgccctg atcatctgtt
    30421 gtatattctg taggaatcgt ttgattagat ggcacactca cagctggcaa ggataatgta
    30481 ggcaatgtaa atgggctgac agaattttcg ttttcttttg atgattcttc ttctatttcg
    30541 tctggctttg gtactgacac atctgtatct ttcttatttt ctgtctcatc aataatgtca
    30601 tattctttat tatcgacgat cagcttgttt gcctggctgt ggtaaagtgc tggatcgatt
    30661 ggaaaactca gatcgatagt ttgtttagtg tttgatgaga tttccaacgt aattttcccg
    30721 ctatttacct gataagcata gccatctcct tcttttcttt ccacatcact taagtaattt
    30781 tcgactagac caatttcttt ttctatcttt tcagcactgg tgttattcgc atttagcttg
    30841 atgttcagtg ctttttttcc agactgatcc gaaatgactt cagctgaaat tgtttctaaa
    30901 actgttggat catctgtagt gtctgctatt gcagataatg gcacaaagtt tgataaactc
    30961 aatattatca gcatgaaaat aactttcagt ttcttccctg tggtcgtcat ttttcccctc
    31021 ctttttcttt ttttctgttt tgacgtgaat agtgaaaatt attaaaataa acaggcgtta
    31081 cataatttta tttattttaa tatgataatt attatacgaa gggcgctaaa gggaatcaca
    31141 ccaattttca taccaattgg tttcatttat gaagttgatt ccttcgatta atgagcgttt
    31201 cattgaaaga aaagggttga gggagaaaaa catatgatca ggttgttgga aggaaactat
    31261 agaaagatgg aaaaaatcat tcgaattctg tccatttctg acaagtggat gactaaaaaa
    31321 gaacttgcca aacgaattgg tagttcagaa tctacatttg taaggtatat cgaagagatc
    31381 aaaatccgct ggggaacgga tgtaacgatt caatcttcac aaaaattggg gtatcgtctt
    31441 gaacatttga atgtaccagc ttatttaaat gttctgatgg aaatgtcgca aagttctaca
    31501 aacagtcgtt tattaaatga gattattgtc aatccgggta aaacgattga gtattattgt
    31561 gaaaaaattt ccatcagccg gtcttctttt acacgtaagt tgaaacaatg caatcaaatt
    31621 ttagaaagct atttattaaa agtgatcgtt gaccaaggtt ttcagttaac tagccttaaa
    31681 agtgagctcc agttgcgtat ctttgttacc tttttctttt taagttacta cggaagacgc
    31741 acgttgccat ttccaatcga aaaaaagaag atcaaagaaa ttatggtcaa gaataattgt
    31801 cagatgaatt tgtttcagga agaaagccca tatgagcaat ctttctttat tatgtatttt
    31861 gttgtgcatc tcatgagaga atcccaagga actccatttg aatggaaagc tggagactct
    31921 tatgtacagt tgataaatat cgataaaaaa gacttcttaa tcttgaaaaa tctcttagga
    31981 aaattgaagt acagcagcta tataaaagta gtgaactatt ttgtcaatac gattttttca
    32041 gaattttcgg agaaagaaaa aaatgaaatc agtgagcaga tacgaaaaaa tatgtcctac
    32101 tgttcgtttt catccgctga ttgcaaagaa aaaaacgaag aatatgttgt tcaattatta
    32161 acgcaaattt attttttctc ccgtattttc ccatacgata cgactcattt gacgatccgt
    32221 gcaacttact ttgcacagca gatgcaacac cgccagatac gtctatatgc tggatttcgt
    32281 gcagaacttc aattttattc cactctactg aatcagaatc tgatttatta tttatctagc
    32341 tatatctttt ggatgcttat cggtaatcct gaaattcacc attatacttc tgataaaaaa
    32401 atactgatta tcagcgactt aagtcttcga catagtcaat atattgaaga atatatcagt
    32461 gatattcttg ctgtgcataa aattcactct gagacaactg ctattactga agatcagctg
    32521 tcgaaataca atcttctcga gtatgatctg atcgttacga atcaacccat tttgaatgcc
    32581 catgttcctc atatattgat tgatgatagt gtgtcattcg caaatgaaga agaactcatc
    32641 cgcttattcg agttatgaat aatcaaaaaa cagctatgta taaaccaccc tgataaaaat
    32701 ttcaggacaa atacacatag ctgtttcttt ttattgtatt ccttcagcaa tccaacgaga
    32761 tttatagcga aagtctttta acaactcttc ttttgtccaa ttatttgcaa tgagatccgt
    32821 caaagtgttc attacgaggg tcatggaaaa gtataggaaa ctaactgctt cttccgacga
    32881 ttgaatggag aaatgattga ttcttaataa ttccagccat ttttcgttca ttggagaatc
    32941 agccgatagt tctctacgtt tggttgtctg ctgatcattt cgctgggtta acaagtataa
    33001 cctttgccaa tagatacttt cgtgatctaa gtcgcagcac cactctaaat atttttcgat
    33061 ccccaaaaag aaatgttgtt tgttttcgct aatgaatttt aagatatctt gatggactat
    33121 gtgagaactg cttttgatcg tatacaaata agcatcttcc aaatcttgaa aatatttata
    33181 aaaagcaccg cgcgacatcc ccatcttgtc aacgatttct gagactttga cttggctgat
    33241 gtgctgtgag caaaatatct ccagtaatag gtcatcgatt ctctgtttct ttgcgttcga
    33301 taaattgaaa aaagtttctt ttggcatcca catctctcct ttatctctga ttatacgtgg
    33361 atagcccttc attgtcatga aggattcatt ccttcttttt ctcttcttct gtcaagtacc
    33421 catcttccat tcgataaacg cgatcactcc attgaatcat acggacatca tgagtaacca
    33481 taatagtcgc tttttgtttc tcatgagctt ctttaactag taatttgacg acttcatatg
    33541 catgatctgt atccaaacta gctgttggtt cgtctgctaa gatcaagctt ggctcattat
    33601 ataaagcgcg agcaattgcc actctctgtc tttctccacc agaaaggtct ttaggataac
    33661 tatttttaag tgactggata tctaaagatt gcagcaagga atcgattttt gttgaatctt
    33721 ctttttttga agtaacttgg tcaactagtt caaattgttc ctttaccgtt aaaaaaggaa
    33781 tcaaattaga gctttggaga ataaatccta tttctttaaa gcgtaaatct gctcgttttt
    33841 tttctggaag ctcagttagt gagtgaccgt tgatcactat ttctccggaa gtcggtgttt
    33901 gcaatcctcc tgaaatggta aggaaagtac ttttacctga accagaagga ccaatgatgc
    33961 tgacgaattc tccttccttt acataaaaat caattgcttt caaagctgag acctttgtat
    34021 gaccagaacc aaatgttttg gtaacttttt tcatcgttaa tatttttttc atttttatta
    34081 acctcctatt gctgtaattg ggtctacttt ggttatcgta cgtatcgaaa acaatccgcc
    34141 tagcattgcg acaatgatca aaatcccact atatagcaac cattgtgacc aaaaaacagc
    34201 aaaaggcatc gcgcttggta aaatcagact agtcaaataa gcaaacaaaa gagcaagcag
    34261 aacgcctacc accccaacaa taaaggactg tgctactaaa gatttagcga tgaagaaatt
    34321 tttgatccct tgtgctttca ttactccaaa aatagcagtc ttctgaagtg tcatcacgta
    34381 catgaaaata ccgaccacgg cagcaaccac taagaacaaa aagtatatca tcgcatttaa
    34441 ggtcatattt tgggcagaat agccaggaat actttcaatg aatgtcccaa tagttaataa
    34501 ctgtagaccc ttcgatgctt tttctttcga aattttagct gaatccttta ttacgatccc
    34561 attgatcggc tgttcttctt cagaagcaaa cggctgttgt ccatacttca aagcagtcca
    34621 cgtatctagg gacgcataga tgacgggaga aactgtgtaa taagtttcag gaaagatacc
    34681 gacaatcgtc aaaggatcgt cgtaactgcc aattttgatc gtatctccaa ttttatagcc
    34741 tgaatcagcc agattttgag agatgatgat ttcattcttt gttttaaatt catgcccttt
    34801 cgtgagtttt ggtaataaaa atgcttcttt cgttgtgccg aaaactgtaa cattttcttt
    34861 gttttttcct ttgattgctc cgctgtacaa tccgattggt gctttctctc ctccttcaat
    34921 gtatttcaga tctcctcttg ttaattgtga agctgcaaat acttgatttg catcctccga
    34981 caaaacaatc tcttgtgcgt cccaatcatc gattgctttt ttaaactctt ctgaaagtcc
    35041 attcgctagt cctgacaaca tgaatacgac atacgcaatc aagaacataa taccgacgat
    35101 caagccatat cgtaatttag aatagcgaat ttctttgata gctagaaaca tttgtttttc
    35161 ctccctatga tagattgata gtgacaaaat gtcacttgtt gaatagtgta actctaaagt
    35221 gacaatttgt cacttgtgtt gtttttttct tatcaataaa ttgtctaaga catctttcga
    35281 taaaacctta acatgagtca taaaaaattt atttaagcct tatttatgct ttccatgata
    35341 aactaaagtg accaaatcaa tgaggtggga aaatgattaa actattagta gtagaagacg
    35401 ataaagtatt atccaataat ataaaagaaa tactcgctgc aatcggcgag ataacacaag
    35461 tttatgatgg tagtgaagga ctgttcgagt tggaaagtgg agtttatgat cttgcaatct
    35521 tggatttgat gatgccggaa atgaatggat atgaagtgtt agcaaaaata cggaaagaaa
    35581 aaatacagac tccagtattg attttgactg caaaagatag tttggaagat aagattgaag
    35641 gatttgaacg aggtgcagat gattatctaa caaaaccttt ctatcgagaa gaactgatca
    35701 tgcgcgtgaa agcattattg aaacgctcgc taggtctctt tgatgaaaat gttctgaagt
    35761 ataaggatat cagttgtgat ttaaaaagca atgaagtctt gtataaagaa caagttttgc
    35821 ccatacaagg aaaagagttt gaattgctcg tttatttttt acaaaataag ggaatcattt
    35881 tgacaaaaga gcagatattt gaccggatct ggggatttga ttctgatacg acgattacag
    35941 ttattgaagt ttatatgagc aacttacgaa aacatctgcg aagtactgga ttggataaag
    36001 aaatcaaaac cttacgtaat gtcggttata tactgcaaag ggtgtgatgc aatgaatgga
    36061 gatattacac gaaagcaacg tagtcgcttt tttgcgacaa atgtagcagc atttgctttt
    36121 atcttttttc ttttgggatt cattacttta cagatattaa accaatctgc atacagagaa
    36181 acagatctgt cattagagaa aataaatttt gattctcgaa cagtacaatt agagatacaa
    36241 agatatcagc aaggcgatcc ctttttaaac ccgactcaaa cggaacaaaa cagaacaaac
    36301 atggaacagc cattaatgga accatcaaat gaccgtttta acactcaagt gatcttgtgg
    36361 tctaaaaatg gtgagatatt gaataaagca gctattggtg ggcgtttcaa ccaaatcaaa
    36421 gaattaaaaa tggatacgaa aaatctgaat accatccaaa atattgaatt aaatgaagac
    36481 agtggctcat ctgccctctc tttccgttcg attacgaaaa aagcggagac gaatcaagat
    36541 ggcgttgcct acatccaaat tttagaaaat acaaatcaaa tcaagaattc attagcgact
    36601 tttcgcacga ttatgatttt gtgtatgatt gttttttgga ttctctctat tggtatcagt
    36661 tattacttgt ctagtctaag catgcgccct attattcttt cttggagaag acaaaaagaa
    36721 tttgttgaga atgcttctca tgaattgcgc acaccgttaa cgattattca aaatagtttg
    36781 gaacatcttt ttaccaagcc taatcatact attattgaag aatccgaaag catcgctcaa
    36841 gcattgtcag agacgaggcg cctcactgga ttgacttcgg atttgctgac aatcgctcgg
    36901 aatgattctg atcagcagct gctttcaaag caaatgatca atacacaaga atatatcgaa
    36961 caacttgtta aaccatttca agagatggcc atcatagatg gaaaagagtt tattctccat
    37021 aacgatgcaa cattagaagt accaattgat gaaaaaaaaa tccatcaagt tttggttatt
    37081 ttgctggata atgcattaaa atatacgaat ccagaagata agatcatttt ggattcatct
    37141 gttaaccata aagaatggct gattcacgta aagaatactg gcccttctat cagtgaagaa
    37201 gatcaagcaa ggatttttga tcgattttat cgggaagatc aatctcgggc gaaagagact
    37261 ggcggatacg ggcttggctt ggcaattgca aaacaggtaa tcgaacagca tcatggacag
    37321 atttctgtat ccaattatct tccacaagga gtcgatttta aagtccaatt gccgttgaag
    37381 cctaaaaaat aatgcttatc cctaaaaaag ggctgtgaca aaaatcttgt cacagccctt
    37441 ttttgttgcg aataaacggt gttccagaag tagcttcttc ggaaataagc cgaaatttct
    37501 ttaagctgac cacttctgtc acaacctctt tagcttacgc ttacgatttt ttattgcggt
    37561 cttcaatgtg tacatcaccg cttgtgtcaa tatccaattc ttctcggcta acatcttctg
    37621 tcactttttc aacatcttca tgttcttctt tatgaatccc aacttcttca cgaataactg
    37681 tatgtttgct tacatctact tgctcttctt tgattgggat agtaatcgtg tcatcttcca
    37741 atttttcgtc tgttccctgt gaagattgat ctgttactgg tttgcgttcg atgacgattt
    37801 cttcacgttt gaccggtact tctaccgttt cagtatcgtt gaccacatgt ttgtggatgt
    37861 cgacttctcc agttgtcaca tcatgtgtat ttacatctaa tttttcttct tttagttgaa
    37921 ttgtatcatc atctagattt ggtttgcgat cgatgtcttc agtcttgctt gtacccattg
    37981 tatcagtctc gccaggtacg tccatcggat cagttcctgg tgtcatattc aatggatccg
    38041 ttgtattttg aaagcctgtc gtgccaccta cgtccattgg atctgttcca gtgccagttt
    38101 ctaatccagt ccccattgtg cttgctgggc taacagtatc ttgtgtatcc attgtatctt
    38161 ctgttgtttc tggctgacga tagcctttta ctgtgatgac aaattttcca tcagaaatat
    38221 ctcctctata ttgatataaa ggatcatttt ctggtgtagc cgtctcagaa tcataggtat
    38281 caaaagaaaa agtatctttt attttttccc ataaagaacg gtcttcttca ccatccatcc
    38341 gattttcatc tgtttcaaca tctataccag acaatccgtc aaatcctagt ctagatgctg
    38401 cttcatcagt agtataaata acgatatcat ctcgttcata cccttcgtta cgcaaccgtt
    38461 caattactga taatgcttct tccctcgtac tatagcttcc gatgaccgtt aaatttttct
    38521 cttccatgta aattcctcct agcaaataga tttatgattg gtatattgtt acttataaat
    38581 ttattctata ggagac
//
LOCUS       DAZBSA010000015        38093 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000015 DAZBSA010000000
VERSION     DAZBSA010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.015"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            457..1185
                     /locus_tag="ACHGMT_000708"
     CDS             457..1185
                     /locus_tag="ACHGMT_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN6834477.1"
                     /translation="MESSSKGKSLYYQLVDVLKERIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELETQGYIYRRHGKGTYVSDLSDKSSDLAGAYSFTEEMRELGRVPNTV
                     ILEFKQMEANKKVAKNLNINLGETVFKLKRLRLADDEPLMVERSYLPAKLFIGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDADPLRIADGAPVLYLSRTTYNVKN
                     EIIEYTDSVARADQFHYKIRHVRK"
     gene            1203..2396
                     /locus_tag="ACHGMT_000709"
     CDS             1203..2396
                     /locus_tag="ACHGMT_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFN6834478.1"
                     /translation="MFELSKDKLKELGAEITTREIKQQPQLWKETAEILAQNQEKLAL
                     FFDQIKKAANGKRVRVIFTGAGTSQYVGDTAVPYLNEYGDNDHFIFHSFGTTDIVSSP
                     KEFLFPDEPTLLVSFARSGNSPESVATVKIANEQIKTLFHLTITCAKEGQLAKNAATD
                     ERNYLLLMPEKSNDAGFAMTGSFTCMLLSALWVFDQTAVEKKESYLQQLIRTGEDLIS
                     RDQEIQKLLPKSFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVIGFVNNQPYTRNYDLDILEEIKDDQITTSVIAIAQPGNIDFTGTSLLLE
                     ASISLPDAYLALPMIMTAQIIALDTSIKVANKPDTPSPTGTVNRVVKGVIIHEYVNKG
                     ETKDE"
     gene            2389..3384
                     /gene="lacD"
                     /locus_tag="ACHGMT_000710"
     CDS             2389..3384
                     /gene="lacD"
                     /locus_tag="ACHGMT_000710"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFN6834479.1"
                     /translation="MNEEKIAAMKSLSTKEGIISALAIDQRGALKKMIKALGKEPTAQ
                     QITEFKELVSAELTPYASAILLDPEYGLPAAKARHQEAGLLLAYEKTGYDATTPGRLP
                     DLLADWSTLRLKEQTADAIKFLLYYDVDEDPEINHLKHVFIERLGSECAGEKRPFYLE
                     LVSYDAKTNDSTSLEYAKIKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVEGYSDG
                     EIAYSKKEAAAFFKEQSDATPLPFIFLSAGVSTALFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGREGAIEWLRTQGRQNIESLNKVINENATPWFDKVKEDSVTAK"
     gene            3381..4322
                     /locus_tag="ACHGMT_000711"
     CDS             3381..4322
                     /locus_tag="ACHGMT_000711"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HFN6834480.1"
                     /translation="MITTVTMNPSIDIAYTLDELRIDQVNRVKTVNKTAGGKGLNVAR
                     VLHLLADDVLATGLVGGQHGAYIKEQLDQSGITHQFLPIKGETRDSIAILHGVNQTEL
                     LESGPVVTEEEQHKFTEHFARLMETTDIFTISGSLPQGFSKSYYAELLALTNKKGTRV
                     LLDTSGQTLLEVLEGKEKPFLIKPNLEELGELVHQTISSATSLDEIQSLLDHPLFEGI
                     NWIVLSLGAKGAIAKVGRHFYQVTIPKIAVVNPVGSGDSTIAGLAHALSKSYTIEETL
                     AFAMACGMANAQQEKTGWIDPVKVDYFRGEINVRKIR"
     gene            4368..6134
                     /locus_tag="ACHGMT_000712"
     CDS             4368..6134
                     /locus_tag="ACHGMT_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase family protein"
                     /protein_id="HFN6834481.1"
                     /translation="MTRFEIKEEFLLDGQPFKILSGAIHYFRVHPTNWERSLHNLKAL
                     GFNTVETYVPWNLHEKEEGTYDFSKILDLSHFLELAQSLGLYAIVRPAPYICAEWEFG
                     GLPAWLLTKECRIRSADPRFMKYVKRYYQTLLPKIVPHLVTHGGNVLMVQVENEYGSY
                     GEEKAYLRQVKKYLEEGGIDVPLFTSDGPWQATLRAGILIDDDVFTTGNFGSKAKENF
                     ADMAQFFEAHGKKWPLMCMEFWDGWFNRWKEPIIKRDPEELADAVKEALQIGSINLYM
                     FHGGTNFGFMNGCSARGAIDLPQVTSYDYDAPLDEQGNPTEKYFALKRMMAENFPEMQ
                     QMEPWIKETIVKKGINLKEKVSFFATLDTISKPIHSTYPQTMEELGQNTGYLLYRTTI
                     KKDAVNEKLRIIDGRDRVHVFLDEEKQAIQYQTEIGETIPLTLEKEDHQIDLLFENMG
                     RVNYGHKLLADTQRKGIRTGVMSDLHFITEWTQYCLPLETTEHIDFSKKWEAGQPAFY
                     RFEAELSEIGDTYLDLSEFGKGIVWVNGTNIGRFWEVGPILSLFVPEGLFRKGQNEIV
                     IFETEGRFSEVIDFVKEPIEKS"
     gene            6151..6630
                     /locus_tag="ACHGMT_000713"
     CDS             6151..6630
                     /locus_tag="ACHGMT_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFN6834482.1"
                     /translation="MAIIGVRIDGRLIHGQVANLWTTKLNIGRIMVVDDEVATNAIEK
                     SGLKLATPAGVKLSILPIEKAAANILAGKYDSQRVLIVAKKPDRILALVEKGVPIKEL
                     NVGNMSQTDETRSITKSINVVDADVEVFKKLDQLGVHLIAQMVPNDKAEDFMSLLIK"
     gene            6643..7533
                     /locus_tag="ACHGMT_000714"
     CDS             6643..7533
                     /locus_tag="ACHGMT_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFN6834483.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLKAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDQHVETMNYKAIERNFLMGALPWALSRMVPVFLAL
                     AFGGGLVQNVVAVLNGDLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHISYLILGFT
                     ITALLATVFSNVQLLGTSVASVVKDFPGTFNSLPMLAVALIGLALATISYKNDQKNDQ
                     HPVVSQQSQSDEGEIEDDEI"
     gene            7520..8329
                     /locus_tag="ACHGMT_000715"
     CDS             7520..8329
                     /locus_tag="ACHGMT_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN6834484.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDDTPELKEMMKTHTQFFNTSNFFNTIITGIDLAIEEKEGVSSKETVSGMKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPVGILIWILAQVAVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKLLPAGIVGGVYWMLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            8345..8749
                     /locus_tag="ACHGMT_000716"
     CDS             8345..8749
                     /locus_tag="ACHGMT_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN6834485.1"
                     /translation="MSRQLVLISHGKFCEELKNSTEMIMGPQENIYTIPLLPEDGADT
                     YKERFLSVTAPLKDYVVLCDLLGGTPANTVSKLIMEGETIELYAGMNLPMVIEFINSQ
                     MIGAEPDFIGSGRESIVSVNQVIAGMGDDEEE"
     gene            complement(8930..9868)
                     /gene="yidC"
                     /locus_tag="ACHGMT_000717"
     CDS             complement(8930..9868)
                     /gene="yidC"
                     /locus_tag="ACHGMT_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFN6834486.1"
                     /translation="MNKWKNWLLGSGLVTIMLFLSGCVRMDSNGNPDTSGIIYRILVH
                     PMGQAITYLVENFNWSYGWAVILMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     ATQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESTFYGIDLGNPSLVLVAIAGIAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGIFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTPAEPAKQTKKQVQAPKNNAKNGRNAGKQQKRK"
     gene            complement(10202..11284)
                     /locus_tag="ACHGMT_000718"
     CDS             complement(10202..11284)
                     /locus_tag="ACHGMT_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFN6834487.1"
                     /translation="MTKNLIVRGAPQEYEIKENAWDSLPEHLYKRHIDNVLILHGKTS
                     WKAVKDVFPDLSITKIFEYYGGECTDPLADSFVSLCKKKDFNGIIAVGGGKVADLGKL
                     IAYKTNLPIIILPTLAATCAAYTPLSVVYNEEGVMLRYDMFPKANDLVLIDPKIILHS
                     PREMMIAGIGDTLAKWYESHAIISQLTIKPIEVQVAEFAALRCREILLEKSAEAMEAM
                     DQQKINQAFIDVVETNILLAGMVGGFGDDYGRTAGAHSIHDAMTVLPDSHQQLHGNKV
                     AYGILVQLMIENKLAEIEHLLPFYHHLNLPTSLADMGLHLTESDYQAVAERACLPDEM
                     IHFMKETITPALVVQKMKELEQVTQG"
     gene            11426..12877
                     /locus_tag="ACHGMT_000719"
     CDS             11426..12877
                     /locus_tag="ACHGMT_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFN6834488.1"
                     /translation="MKKGINILLVALMFLLVFLPVKVQGAEKDEIYERIKQSGELVVG
                     LSADYAPYEFHANVDGKDKIVGFDVSIAEKIAKDMGVKLKVEELGFDALLGALKTGKI
                     DLVISGMAVTEERLQEVDFSDPYFVVQQKVLVRKEDKNKFKTTADFSGLAVGAQKQTT
                     QEELVKNELTGAELTSLQKVPDLINNLLNEKVEGVVLEQPVAEAYAQQNKKVALTPVK
                     FAQGEKTTAIAMSKNTPELKNHVNASIKTINDDGLLKDYQKQANQLMFQDDQSFLQKY
                     GPYYLIGTKYTILLAFVGVLFGMVFGSLLALMKLGKNKFLKALAIIYIEYVRGTPLLV
                     QIFIVYFGTGVLGLDLSKLVAGCIAVSLNSAAYVAEIVRAGINAVNKGQMEAARSLGM
                     DQKEAMRFIIFPQAIKNILPALGNEFVTIIKESSVVSVIGVSELIFQTGVVQGASFKP
                     FLPYVLVSLIYFVLTFSISRILALVERRMGLNA"
     gene            12870..13592
                     /locus_tag="ACHGMT_000720"
     CDS             12870..13592
                     /locus_tag="ACHGMT_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFN6834489.1"
                     /translation="MLNVKKLSKSFGENEVLKSIDLSVKEGEVVVIIGPSGSGKSTIL
                     RCMNLLEEPTSGEIFFKGQNITAPDSDINQIRQKMGMVFQSFNLFPHMNVLENLTITP
                     VKIKKEDPKKAKEHALSLLNQVGLKEKVDSYPTSLSGGQQQRVAIARALAMNPDVMLF
                     DEPTSALDPEMVGEVLAVMQDLAKKGMTMVVVTHEMGFAKEVADRVIFMADGIIQEEG
                     TPKEIFENPQNSRTQDFLNKVL"
     gene            13612..14763
                     /locus_tag="ACHGMT_000721"
     CDS             13612..14763
                     /locus_tag="ACHGMT_000721"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFN6834490.1"
                     /translation="MDKSKIARRFQQPAENLLMNIAILAKEKEDIIDLSIGDPDLITD
                     PTIIEAAYTDVKNGATNYTAPDGSKEFLSTVVDFYQKKYGLSVECNQVRATVGAMHGM
                     YLVLQALLDPEDEVIIHEPYFSPYREQVILSGGKPVFVPTYEEKGFQLDVEDLKAAIT
                     DRTKVIVINSPNNPTGAVFSESTLKKIAEVAIENELYIISDEIYEDFAFKEKFVPMAR
                     FAPDNTITVSGVSKGFAMTGWRLGYMIAPAYINQIAGLINENITYSAPTASQRGAIYA
                     LKHYEQLVPPVVEVFKERLAYIEKRVAEIPYLSLSSVSGSMYAFINISKSGLDSVTFV
                     EEVLEKSGVLFVPGKAFGETIGDNYIRLAATQPLEILGEAFDRLAKLNF"
     gene            complement(15111..15386)
                     /locus_tag="ACHGMT_000722"
     CDS             complement(15111..15386)
                     /locus_tag="ACHGMT_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFN6834491.1"
                     /translation="MNKIKMNVQGRVQSVGFRYMTKIVADQLGITGTVKNEDDGSVTI
                     EAAGDDEAIKKFVEEVKRSPSPSGRVQYVDLQENPMMEDRKKFVVIG"
     gene            15494..16258
                     /locus_tag="ACHGMT_000723"
     CDS             15494..16258
                     /locus_tag="ACHGMT_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFN6834492.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKGEYLLEGFHLIEEAVAS
                     NAKVNELFITQRGLTEWASWLEENQALSVTLVSEEVMATVSDLPTPQGIMAVVEAEKR
                     EIPNFEGGWLLLDNVQDPGNVGTMIRTADAFGLSCVVLGKGTADVYSTKVLRSMQGSN
                     YHLPIIQEDLTKLLPILKEQGFITYGTELNEEAVSLNDIQTTENYAIVMGNEGQGVRP
                     EVLALTDKNVYIPIQGNAESLNVGVAAGIMLYHFSV"
     gene            16382..16885
                     /locus_tag="ACHGMT_000724"
     CDS             16382..16885
                     /locus_tag="ACHGMT_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFN6834493.1"
                     /translation="MTEKWREDEEYMSYINDLLETDAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYKLAKKWGGDARATARAGLLHDLFYYDWRTTKFDEGTHAYVHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMTASMKQKWQRY
                     FGKEQSI"
     gene            17150..17797
                     /locus_tag="ACHGMT_000725"
     CDS             17150..17797
                     /locus_tag="ACHGMT_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFN6834494.1"
                     /translation="MEKTIKPYQYYFIKREVEQLLSAYSSVNDPKTVQTVQALAAEKI
                     HEVLGHPNTAVNEFMEKVLDVTLKKEKAERLLEELKVIVLPFIQPTKPQIDKLFRKVK
                     KLKQPDWNQLDLREHTYIGWNDPGSQKKFMIYYEEEKLKGISGTLSPTINKGICSICQ
                     RTSNVSLFLSTTKTGSDGTYTKKGNYICHDSDQCNQQLVQLAPFYEFTERVKRER"
     regulatory      17840..17907
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            17958..19226
                     /locus_tag="ACHGMT_000726"
     CDS             17958..19226
                     /locus_tag="ACHGMT_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFN6834495.1"
                     /translation="MENRLPDPLKVIIYMIKWLLITAILGIFMGSLSAFFLTSLTDVT
                     AIRLEHPWLLFLLPISGGLFAYLYTTYGGNSSRGNNLVIDQGNGGNEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALAENFARWLKLNKIEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEALFPSFFAALFANFVTESFNVKHTHYQMGAIPAWDPS
                     LFFKLLLAGIAFGIIGWLFSRSIVWLKAWYAKRIPHPVLRNFIGGAVVVLVVVILQTQ
                     RYLGLSLPLLQDAFNGSAHPYDFLGKLGFTVLSLGAGYQGGEVTPLFEIGATLGATLA
                     PILHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGGQAASYFFMICLISFMCSGN
                     SGIYSAQKVEVKKGILFLPILGRISRQKEK"
     gene            complement(19403..20605)
                     /locus_tag="ACHGMT_000727"
     CDS             complement(19403..20605)
                     /locus_tag="ACHGMT_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFN6834496.1"
                     /translation="MNLTRDLIFLVIYILGMSGILYWRKRQSDPALFFYCGTWTFMMM
                     VTYFVVLEVFYHTFYFLIPLLFFSIFAFFYFKEKRRLINGVLFNTFLIVFGGYLGWLL
                     IETQNIFIGGLLALIGIPILILLAIGIYILIIFLFWNGVVVMKKESRSLANLLTLLLG
                     VALTIFLILDYTLVHWLPQWANALFAVIPFIFAYLLIVFFNFMTVSILYQFNRPRYNQ
                     DYIVVLGAGLIDGERVSPLLAKRINKAIAFYWAQSKATLNPPVLLMSGGKGTDEKVAE
                     AIAMKQYALEKGIPERDILVETNSTTTLENMLFSKEIMDQQMNGPYRAIFTSNNYHIF
                     RAGLYARQAKLNANGIGAKTALYYLPNAILREFIAILVLHKKRHLIICGLMIGFAVFS
                     ALILKFAQ"
     gene            21132..21857
                     /locus_tag="ACHGMT_000728"
     CDS             21132..21857
                     /locus_tag="ACHGMT_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTC domain-containing protein"
                     /protein_id="HFN6834497.1"
                     /translation="MNKKRFKRIERKFALTYDQAKYLKTLFPKQVVRDKYSDHFISSL
                     YFDTNDHHFLKEITTEKSYREKFRIRKYGDPQDISPIYLEIKRKFQGMTDKRRIKLAH
                     HELDDMLENIELAETTFSNLEEEYIFNEIQWLFRRLALEPKVYIYYLREAFVNRDSED
                     LRITFDQNIHYRLMNLTTWEHCTEDNLVAPEIDVLMEVKTTGEIPEWLQVGLDRMGKK
                     EIKFSKYKQVYKRYIQEDPLYVQ"
     gene            21847..22521
                     /locus_tag="ACHGMT_000729"
     CDS             21847..22521
                     /locus_tag="ACHGMT_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HFN6834498.1"
                     /translation="MFNSVLKNEPSQLTLDDFFLCMVVSILLGILIAYTHMYQNNCTK
                     NFMFALIGLPTLVHTVILLINGNLGTGIAVLGAFNLVRFRSAQGNSRDIFSIFWATSI
                     GLATGTGYIVYALFVSSIVAAIQVIIFTLPIKNKHTVFNTEKTIKLKVSKSLFLSSII
                     EEKIAEYTENCNLKKVKINKETCSLHYIVKVKTSMLEKELVRTIMDHPEVISITVGMD
                     KSNKLL"
     gene            complement(22809..23093)
                     /locus_tag="ACHGMT_000730"
     CDS             complement(22809..23093)
                     /locus_tag="ACHGMT_000730"
                     /inference="COORDINATES: protein motif:HMM:NF024076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HFN6834499.1"
                     /translation="MRQKCFLLLSHSQKVKPIFWLSIWDGFFKVSFHFPEKTREKLVT
                     LSLSEGTKEKIKTLQANGNKRKHLSVIFDVDHLEQLADIYQLAEFKKQNG"
     gene            complement(23387..24694)
                     /locus_tag="ACHGMT_000731"
     CDS             complement(23387..24694)
                     /locus_tag="ACHGMT_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFN6834500.1"
                     /translation="MFKKNTRFIVLLASLILCITSFLPSATVQADDAFKINAKAAFAV
                     DAESGKILYDQDGEKTMGIASITKIIGLYIVLEQIKEGKLSWEDKVAISDYAEKLSVV
                     PDLSNVPLHKENDYTVKELVDSAFIQSANASMVALAEKVAGSESKFLELMKKQLKDWG
                     IKDATIVNASGLNNSYLGENRPENTGETDENQLSAKDVAIVARHLILDYPEVLKITST
                     TNQTFGENTQSPVEMVNWNWMLPGFVNYHEGVDGLKTGTTDFAGACFVGTINKDGRRI
                     ITVVLNADNHEENPSARFNETSRLMDYCYDNWSEKELGKANASIPSLKDIKVKDGKET
                     SVNVALKSPVKVWVENGMDTGKLTITPTIDKKKVTDDQVTAPINKGTTVGSATIQLAD
                     DKLGYLEANQEPSTDIITTSTVEKANIFVIGWRHVTDFFSNLF"
     gene            25183..26289
                     /locus_tag="ACHGMT_000732"
     CDS             25183..26289
                     /locus_tag="ACHGMT_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834501.1"
                     /translation="MAKKEKELLSQLTSEGTKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKITSLLDDPSFWRSATTSVAVIVSPEQVFIHRLGI
                     PVDDQYYVSDLPYLLAIIKNGHFNYSYYLLGLNRDSMKLYEVTNKQVKEVALPEDAPR
                     DVVTALGSELTGGGLNYASNGSEGTTFHGVNPKDEEVEIDWVNYYQAVDDYLKDTFEN
                     PEKWPIFLFALPENQTMFKKVAKNPYYRSDVAISASPAQLSLKEIEAGAKKLSQELAV
                     KEASDYNKLLDKKFIDQLVDIIPAAKEGKISHLFIATSNLADGFGEDPETEYDRRQVL
                     NELADNVITNGGQVFILEQKDAPDEKSLVAILRY"
     gene            26880..27080
                     /locus_tag="ACHGMT_000733"
     CDS             26880..27080
                     /locus_tag="ACHGMT_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN6834502.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            27227..30016
                     /locus_tag="ACHGMT_000734"
     CDS             27227..30016
                     /locus_tag="ACHGMT_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFN6834503.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIEAGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSSHTGMDTSRFIYHVLEEMKENPEPLDLSLEIIDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIMEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPGQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHAFFADVAHRGTGFLARNQSFYKQDFVRFLQKKIKQSNFLIVNHAFLVQETQRKEP
                     LLPASPYLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICVAEGLMNLVSSKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAMDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLLDFQAAMLPSTVWYPRYDHILYIGGTLKIGP
                     NRQYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLEAALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAIDEI
                     YQDIHEFLKKNEEGS"
     gene            30063..30590
                     /locus_tag="ACHGMT_000735"
     CDS             30063..30590
                     /locus_tag="ACHGMT_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFN6834504.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            30611..31801
                     /locus_tag="ACHGMT_000736"
     CDS             30611..31801
                     /locus_tag="ACHGMT_000736"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFN6834505.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            31841..33139
                     /gene="asnS"
                     /locus_tag="ACHGMT_000737"
     CDS             31841..33139
                     /gene="asnS"
                     /locus_tag="ACHGMT_000737"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFN6834506.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            34073..34747
                     /locus_tag="ACHGMT_000738"
     CDS             34073..34747
                     /locus_tag="ACHGMT_000738"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFN6834507.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWREDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQKAGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            34744..35085
                     /locus_tag="ACHGMT_000739"
     CDS             34744..35085
                     /locus_tag="ACHGMT_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834508.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVGQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            35115..35474
                     /locus_tag="ACHGMT_000740"
     CDS             35115..35474
                     /locus_tag="ACHGMT_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFN6834509.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     AFLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            35734..35856
                     /locus_tag="ACHGMT_000741"
     CDS             35734..35856
                     /locus_tag="ACHGMT_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834510.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAVAMIEEKKKE"
     gene            35998..38079
                     /locus_tag="ACHGMT_000742"
     CDS             35998..38079
                     /locus_tag="ACHGMT_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFN6834511.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNELIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
BASE COUNT        12391 a         6635 c         8074 g        10993 t
ORIGIN      
        1 aaacttacgc cataaggaaa ctagaaaaag acagaattat cataaaaaat ttttgttatt
       61 tttaaaaatg tacaacaaaa tttcgactat cagaccggca tgagagaaaa gttatctaaa
      121 aaataaaaaa tcctacttta tttagagaaa gccattttta tttacatagt agtctattag
      181 gcccatttgc ttgtcgatag tagaaatagc cacagatgta taaagaatat tttttcttta
      241 cttctctaga ccatttagct gaatctttgc caagattcag cttttttttg ttttttattt
      301 gtcaactggt atttttgagg tatcttttga taccagttct aaattacctt gaaaccttgt
      361 tataatagct tttttacgcc aaaaaccctt tgacaaatga ggaatatgtg ggtattcttg
      421 tgttgtggtt ataaccagta aagaagaggt atttttatgg agagctcaag taaaggaaaa
      481 tcattatatt atcaactagt tgacgtattg aaagaacgta tcgaaacaca gatgacacct
      541 catgacaaat tgttatctga aagagaactt tgtcagcatt atggagtgag tcggacaacg
      601 gttcgccttg ctttgcaaga attagagacg caagggtata tttatcgccg acacggaaaa
      661 ggaacatatg tgtctgatct atcagataag tcgtctgatc tggcgggagc gtatagtttt
      721 acagaagaga tgagggaatt gggacgggtg cctaatacag tcatcttgga attcaagcaa
      781 atggaagcaa acaaaaaggt tgccaagaat ctaaacatca atttaggcga aacagttttc
      841 aaattgaaaa gacttcgttt agcggatgac gaaccattga tggtggaacg ttcttattta
      901 cctgcaaaac tatttattgg tcttgatctg ccattacttg aaagtaagcc attgtatgat
      961 gtgttttctc aggattatga acagcaagtc caattagcag atgaagaatt ttatgcaagt
     1021 atcgttcaaa agaaagatgc tgatccgctt cgtatcgctg atggtgcccc ggtattatat
     1081 ctaagccgaa cgacctataa tgtgaaaaat gaaattattg aatatacgga tagtgtagcg
     1141 agagcagacc agtttcatta taagatccgc cacgtccgaa aatgattgag agggaaaaca
     1201 acatgtttga actatcgaag gataaattaa aagagttggg tgcggaaata actacgcgag
     1261 aaatcaaaca gcaaccgcaa ttatggaaag aaacagctga gatattagca cagaatcaag
     1321 aaaaattagc attgtttttt gaccagataa aaaaagcagc aaatggcaaa agagttcgag
     1381 tgatttttac tggtgcaggc acatcacaat atgtcgggga tacagcagta ccttatttaa
     1441 atgaatacgg tgataacgat cactttattt ttcatagctt tggtacgaca gatatcgtgt
     1501 cttcaccaaa agaatttcta tttccggatg aaccgacatt actggtgtca tttgcacgta
     1561 gtggaaatag tccagaaagt gtggcaacag tcaagattgc caatgaacaa atcaagacat
     1621 tgtttcattt gacgattact tgtgctaaag aaggtcagtt agcaaagaat gcagcgacag
     1681 atgaacgaaa ttatctttta ctgatgccag aaaaatcaaa tgatgcagga tttgccatga
     1741 caggaagttt tacgtgcatg ttgttgagtg cgttatgggt attcgaccaa actgccgttg
     1801 agaaaaaaga aagttatctt caacaattga tcagaactgg agaagatcta atttcacgag
     1861 atcaagaaat ccaaaaatta ttgcctaagt catttgaacg tatcgcctat cttggttcag
     1921 gaagtttggc tggtgcaacg agagaagcac aattgaaaat attggaattg acagccggta
     1981 aagtggcaac gatctttgat tcttctatgg gcttccgcca tggaccaaaa tcgtttttaa
     2041 atagcgaaac acttgttatt ggttttgtta ataaccaacc gtatacaaga aattatgact
     2101 tagatattct agaagaaata aaagatgacc aaattaccac gagtgtcatc gcgatcgccc
     2161 aaccaggaaa catcgatttc acaggtacaa gtcttttgtt agaagcttcg atcagcctac
     2221 ctgatgcgta tcttgcctta cctatgatca tgacagccca aatcattgcg ctcgatacgt
     2281 cgataaaagt agcgaataaa ccagatacac catcgcctac aggtacagtc aatcgagtgg
     2341 tcaaaggtgt cattatccat gaatatgtga acaaagggga aacgaaggat gaatgaagaa
     2401 aaaattgcag ctatgaagag tttatcaaca aaagaaggaa tcatcagtgc attggcaatt
     2461 gatcaacgcg gtgctttgaa aaagatgatc aaagcgttag gaaaagaacc aacggcgcaa
     2521 cagatcactg aattcaaaga attagtatca gcagaattaa cgccttatgc ttctgctatt
     2581 ttactagatc cagagtatgg attgccagct gctaaagcca gacatcagga agcaggacta
     2641 cttttagcct atgaaaaaac gggttatgat gcgacaactc caggacgtct gcctgattta
     2701 ttagcggatt ggtctacgct tagattaaag gaacaaacag ccgatgcgat caagtttttg
     2761 ctttattatg atgtagatga agacccagag atcaatcatt tgaaacatgt atttatcgaa
     2821 cgtttaggca gtgaatgtgc aggtgaaaag cgtccgtttt atctagaatt ggtttcgtat
     2881 gatgccaaaa ccaatgacag cacttcatta gaatatgcga agatcaaacc gcataaagtg
     2941 atcgagatga tgaaagaatt ttctgatcct aaatataaag tggatgtatt gaaagtagaa
     3001 gtgccagtca atatgaacta tgtagaaggt tattctgatg gtgaaattgc gtatagtaaa
     3061 aaagaagcag cggcattctt caaggaacaa agtgatgcaa caccgttacc atttatcttc
     3121 ttgagtgcag gggtttcgac tgcattgttc caacaaacat tgatctttgc tcatgaatct
     3181 ggttcaacat tcaacggtgt gttatgcgga cgtgcgactt ggaaagatgg agtcaaagca
     3241 tatgttgaaa atggtagaga aggtgcgatt gagtggttga gaacacaagg gcgacaaaat
     3301 atcgaatcgt tgaataaagt gataaatgaa aacgccaccc cttggtttga caaagtaaaa
     3361 gaagattcgg tgacagcaaa atgatcacga cagttaccat gaatccttca attgacatcg
     3421 cttacacgtt ggatgaatta cggatcgacc aagtcaatcg ggtcaaaacg gtcaacaaaa
     3481 cggctggagg aaaaggcttg aatgttgctc gtgtccttca tttattagca gatgatgttt
     3541 tagctactgg attagtcggc gggcagcatg gagcatatat caaagaacaa ctcgatcaat
     3601 cagggataac gcatcaattt ttacctatca aaggggaaac gagagattcg attgctattt
     3661 tacatggagt gaatcaaaca gaactattgg aaagcggtcc tgttgtgacg gaagaagagc
     3721 aacataaatt taccgagcat tttgcacgac taatggaaac aactgatatc tttacgattt
     3781 cgggtagtct gcctcaagga ttttctaaaa gttattatgc agagttactt gccctaacga
     3841 ataaaaaagg aacacgagta ttgttagata cttctggtca aaccttgctt gaagtcttgg
     3901 aaggcaagga aaaaccattt ttgatcaaac ctaatttgga agaattagga gagttggtcc
     3961 accaaacaat ttcttcggct acttcgttag atgaaatcca atcattattg gatcatccgc
     4021 tttttgaagg aatcaattgg atcgttcttt cgctaggcgc aaaaggagca atcgctaaag
     4081 tagggagaca cttctatcag gttacgattc ctaaaatcgc ggtggtgaat cctgtggggt
     4141 caggagattc gactattgca ggcttagcac atgctctatc aaagagctat acgattgaag
     4201 aaacacttgc gtttgcgatg gcctgtggga tggcgaatgc acaacaagaa aaaacaggat
     4261 ggatcgatcc tgtgaaagtc gattatttta ggggggaaat caacgtcaga aagattcgtt
     4321 gattataaaa ttagaaagcg gtttctaaaa acgaggagga aatttgcttg actagatttg
     4381 aaataaaaga agagtttttg ttagacggtc aacctttcaa aattttatct ggtgccatcc
     4441 attatttccg agtgcatccg acgaattggg agcggtcatt gcacaatttg aaagcgcttg
     4501 gatttaatac tgtggaaacc tatgtgcctt ggaatctcca tgaaaaagaa gaaggaacct
     4561 atgatttttc gaaaatattg gatctttccc attttctaga gttagcgcaa tcccttggat
     4621 tatacgcaat cgtacgtcca gccccttata tttgtgcaga atgggaattt ggtggcttgc
     4681 ctgcttggct attgacaaag gagtgccgta ttcgttcagc ggatcctcgc ttcatgaagt
     4741 atgtcaaacg gtattaccaa acgctactac ctaaaatcgt tcctcatcta gtcactcatg
     4801 gtggcaacgt cttaatggtg caggtagaaa atgagtacgg ttcgtatggt gaagagaaag
     4861 catacctgcg ccaagtaaaa aaatacttgg aggaaggtgg aatcgatgta ccgctattca
     4921 cttctgatgg accttggcaa gccacattaa gagcaggcat tttgattgac gatgatgttt
     4981 ttacgacagg gaattttggc tcaaaagcaa aagaaaattt tgcagatatg gcccagtttt
     5041 ttgaagcaca tggtaaaaaa tggccgttga tgtgtatgga attttgggat ggctggttta
     5101 atcgttggaa agagccgatc attaaaagag atccggaaga attagcggat gcagtcaaag
     5161 aagcattaca gatcggcagt atcaatctct atatgttcca tggcggaacc aattttgggt
     5221 ttatgaatgg ctgttcagcc agaggcgcga ttgatttgcc gcaagtcact tcctatgatt
     5281 acgatgcacc attggatgag cagggaaatc caacggaaaa atattttgct ctgaaacgaa
     5341 tgatggcaga aaattttcca gaaatgcagc aaatggaacc atggataaaa gaaacaatcg
     5401 tcaaaaaagg aatcaacttg aaagaaaaag tcagcttttt cgcaacactg gataccatca
     5461 gcaagccgat ccactcgacc tatcctcaaa caatggaaga attgggtcag aatactgggt
     5521 acctattgta tcgaacaacg atcaaaaaag atgcggtcaa tgaaaaatta cggatcatcg
     5581 acggacgtga tcgggtccat gtttttctcg atgaagaaaa gcaagcgatt caataccaaa
     5641 cagagatcgg tgagaccatt ccattgacac ttgagaaaga agaccatcaa attgatctat
     5701 tatttgaaaa tatgggacgg gtcaactatg gacacaagct gctagctgat acgcaaagaa
     5761 aaggaatacg tacaggagtg atgagcgatc ttcattttat cacagaatgg acacagtatt
     5821 gtttaccttt agagactaca gaacacatcg atttttcaaa aaaatgggaa gcaggtcaac
     5881 cagcatttta ccgctttgaa gcggaactgt cagaaatcgg tgatacttat ttggacctta
     5941 gtgagtttgg gaaagggatc gtttgggtga atggcacgaa tattggtcgt ttttgggaag
     6001 taggaccaat tctttcactg tttgttcccg agggtttatt tagaaaagga caaaatgaaa
     6061 tcgttatctt tgaaacagaa ggtagattca gtgaagttat agattttgtc aaagaaccaa
     6121 tagaaaaaag ttagaaaagg agaatggatg atggcaatta ttggagtaag aattgatgga
     6181 cgtttgatac atggacaagt ggcaaatctt tggacgacaa agttgaatat tggtcggatc
     6241 atggtcgtag acgatgaggt agcaacaaat gcgatcgaaa aaagtgggtt aaagttggca
     6301 acgccagctg gagtcaaatt aagtatttta cctattgaaa aagcagccgc aaatatttta
     6361 gcaggtaaat atgattctca acgagtgctg atcgtagcga aaaaaccaga tcgtatctta
     6421 gcgctagtag aaaaaggtgt accgatcaaa gaattaaatg tcggcaatat gtcacaaaca
     6481 gatgaaacac gttccatcac aaaatcaatc aatgtcgtag atgcggatgt agaggtcttc
     6541 aagaaattag atcagcttgg cgttcatttg atcgctcaaa tggtgccaaa tgacaaagca
     6601 gaagacttta tgagtctttt aatcaaatag gaggaatgat tcatggatat tcaatggtgg
     6661 cagatattat tattaacatt atatgcaggg tatcaaattt tagatgaatt acagatttat
     6721 tcttcaatga gcgccccagt ttttgcagga ttatatgctg ggttagtaat gggtgattta
     6781 aaagcaggtt taatcattgg tggaagtatg cagttgactg ttttaggggt agggacattt
     6841 ggtggtgcat caaaaatcga tgccaactct ggaacgatct tagcaacagc tttctccatt
     6901 tctctaggga tgaacccaga acaagcgatt gcggccatcg cggttcctgt agcaagtttg
     6961 atgatccaat tagatatttt ggcacgtttt gcgaatacgt attttgcaca tcggatcgat
     7021 caacatgtgg aaacaatgaa ttacaaagca attgaacgga acttcttgat gggggcatta
     7081 ccttgggcac tttccagaat ggttcctgta ttcttggccc tagcattcgg tggtggctta
     7141 gtccaaaatg ttgtggctgt tttaaacggc gacctaaaat ggctaggaga tggcttatct
     7201 gttgcagggg cagtcttacc agcagttggg tttgcaattt tactacgcta cctaccagta
     7261 aagaaacaca tttcttatct gattttagga tttacgatca cagcgttatt agcaacagtg
     7321 ttctcgaatg tccaattgtt aggaacaagt gtggctagcg tggtcaaaga tttcccagga
     7381 acattcaatt cattaccgat gcttgctgtg gcattgattg gtctagcact ggcaacgatc
     7441 agttataaga atgatcaaaa gaatgaccaa catccagtag taagtcagca atcacaatct
     7501 gatgaggggg aaatcgaaga tgacgaaatc taattataaa ttgaccaaag aagattttaa
     7561 gcagatcaat cgtagaagtt tgtttacttt ccaattaggt tggaactatg aaagaatgca
     7621 aggttcaggt tatctgtata ctatcttgcc acaacttaga aaaatctatg gtgatgatac
     7681 accagaatta aaagaaatga tgaaaacaca tacgcaattt tttaatacgt ctaatttctt
     7741 taacacgatc attactggga tcgatcttgc tatcgaagaa aaagaaggag tctcttcaaa
     7801 ggaaactgtt tctgggatga aagcaggatt gatgggttcc ttcgctgcaa ttggtgattc
     7861 tgtatttgcg gcattgatcc cgaccatatt tggagcttta gccgcaaata tggcaacaga
     7921 tggtaatcca gtaggtatcc ttatctggat ccttgctcag gtcgctgtca tggtctttcg
     7981 ttggaaacaa ctagaatttg cctacaaaga agggatttct ctagttacca cgatgcaatc
     8041 acgcttgact gcattgacag atgcggctac tttactagga gttttcatgg ttggagcgtt
     8101 agtagcaacc atgatcaatg ttaaattctc atgggcacca acgattgggg atgtaacgtt
     8161 gaatatgcaa aactatatcg atatgatctt acctaaactt ttaccagctg gaatcgttgg
     8221 tggcgtttat tggatgttag gtaagaaaaa tatgacacct actcgagcaa tattcatcgt
     8281 attgatcgta tgtgtagcat tatctgcatt aggtgtgatt tcgaaataag ggagaattgg
     8341 aaagatgagt agacaactag tcttgattag tcatggtaaa ttttgtgaag aactaaaaaa
     8401 cagtacagaa atgatcatgg gaccacaaga aaatatctat acgatccctt tattgcctga
     8461 agatggcgcg gacacgtata aagaaagatt cctctccgtt acagcaccat tgaaagatta
     8521 cgtagtgctt tgcgatctgt tgggtgggac acctgcaaat accgtttcca agctaatcat
     8581 ggaaggtgaa acgatcgaac tatatgctgg catgaatttg ccgatggtca tcgaattcat
     8641 caattcgcaa atgattggag cagaaccaga ttttattggt tcgggtcgtg agagtatcgt
     8701 ttcagtgaac caagtgattg ctggcatggg agacgatgaa gaagaataat caagcacaac
     8761 ttcttgactg gtgatacaac tggatataaa tacggatgta taaagaggcc gtgtataaaa
     8821 aagccaacgt accgcgaaat atagttttct atatttcaca gtacgttggc ttttatcgtc
     8881 ataaaatcag aaatgatctg ggtcgatcga agaagctagt tacaaataac tatttacgct
     8941 tttgctgttt accagcattc cgaccattct tcgcattgtt ttttggtgct tgtacttgtt
     9001 tttttgtttg cttcgcaggt tcagcaggtg tgacatcttt acgaggcgtc accacttgtt
     9061 ttggcggatt ttgttttaat tcttccgcaa cttgcgcttt taatcttggt ttcatgatga
     9121 cattcgtaat gaatgtttgt agacaactaa agatcccacc gactacccag taaagagtga
     9181 caccagctgg ggcactgatc gacatgaaga cgatcatagc gggtgatacg atcaacatcg
     9241 tacgcatcgt tttcttttgt tcttcaggga taccgatagt tgagatatag ccttgaagta
     9301 aataagcaat accggcaatc gctactaaaa ctaagctagg gttacctagg tcgatcccgt
     9361 aaaaggttga ttcacggatg ccttctgtat agcgagcagt gaaatatagc gcagaaaaga
     9421 tcggcatttg gatcagaagc ggtaaacaac cgataccacc agtcatactt aagccatttt
     9481 cacgataaac ttgttgcatt tcagcttgtg cagccatttg ttcttctcga gttgtcgcag
     9541 ctttcaattt ttgttgcgta gcttcaactt gtggtttcaa tgcttgcatc ttttcagatt
     9601 ggatcattgt ttttttcgat tgactaatac ctaatggcaa aatgatgata cgaacgatga
     9661 ctgtcatcaa gataacagcc cagccgtagg accaattaaa gttttccact aaatatgtaa
     9721 tcgcttgtcc cattgggtga accaatatac ggtaaatgat tcctgaagtg tcagggttac
     9781 catttgaatc catgcggaca caaccggaca agaaaagcat gatcgtgaca agcccagagc
     9841 ccaataacca atttttccat ttattcataa aatacttcct cttttataat ttttacacaa
     9901 gcttaactat actaaaaaag cgctcataat tcaatcaact tcatagaaat ttcatagaaa
     9961 taaccgattg gaggatcaaa aaagacgctt aatgttaaaa agaagaatcc agctaaaaat
    10021 acttcagtat aaagcatact tagcacctcc gttctctaaa ataagatgag caacggatat
    10081 ttccttgaaa tcataatttt agcgcaaaaa aaagaagtgg aatgtcaatt ttttccactt
    10141 cttttttttt accattgagc cgatccataa aaaagctgac tgccatggta taaggggcta
    10201 actatccctg tgtcacttgt tctaactctt tcattttctg gacaaccagt gctggtgtga
    10261 tcgtttcttt catgaagtgg atcatttcat caggtaaaca tgcacgttca gctacggctt
    10321 gatagtcact ttctgttaaa tgtaaaccca tatcagctaa actcgttggt aagttcaagt
    10381 gatgataaaa cggcagtagg tgttcgattt ctgctaattt attttcgatc atcagctgga
    10441 caaggatacc gtatgcaact ttgtttccat gaagttgctg atggctatca gggagtacgg
    10501 tcatcgcatc gtgaatggaa tgagctccag ctgttcggcc gtagtcatcg ccaaacccac
    10561 caaccatacc agccaataaa atgtttgttt ccacgacatc gataaaagct tgattgattt
    10621 tttgctggtc catcgcttcc attgcctctg cacttttctc taaaaggatt tcgcggcatc
    10681 gtaacgctgc aaattccgct acttggactt cgataggctt gatagttaac tgagagatga
    10741 ttgcgtgaga ttcataccat tttgctaagg tgtcaccaat accggcaatc atcatctcgc
    10801 gaggggagtg caaaatgatc tttggatcga tcaacactaa atcatttgct ttagggaaca
    10861 tatcatagcg aagcatcact ccttcttcat tatagaccac actaagtgga gtataagctg
    10921 cgcacgtcgc agctaaggta ggtaagatga tgattggtaa gtttgtctta taggcaatca
    10981 attttcctag atctgctact ttcccgccgc cgactgcaat aataccattg aaatccttct
    11041 ttttgcataa agaaacaaaa ctgtcagcaa gtggatcggt acattctcca ccataatatt
    11101 caaaaatttt tgttattgat aaatctggaa aaacatcttt tactgctttc caagatgttt
    11161 ttccatggag aatcaaaacg ttgtcaatat gccttttgta aaggtgttca gggagtgaat
    11221 cccatgcgtt ttcttttatt tcatactctt gaggggcacc gcgaacaatt aaattttttg
    11281 tcatataaaa aacttcttcc attatttttt taaattaatc tttttttatt ataatagtta
    11341 tgctagtata ttagcaagtt atttatgaat atttattctt ttatagcttt tctgattaca
    11401 ttattattca aaggtggcca aatggatgaa aaaaggaata aacatattat tagtggcgct
    11461 gatgtttcta ctggtttttt taccagtaaa agtacaaggt gcagaaaaag atgaaattta
    11521 cgagcggata aaacaaagtg gagaattagt cgttggccta tctgcagact atgcgcccta
    11581 tgaattccat gcgaatgtgg atggtaagga taaaatcgtt ggttttgatg tatccattgc
    11641 cgaaaagatc gcaaaggata tgggtgtgaa actaaaagta gaagaacttg gttttgatgc
    11701 attattaggt gcgttgaaaa ctggcaagat cgatttagtt atatctggta tggcggtgac
    11761 tgaagaacgt ttacaagaag ttgacttttc tgacccatat ttcgtggtac agcaaaaagt
    11821 actcgttcgt aaagaggaca aaaataaatt taagacgaca gccgactttt ctggcttagc
    11881 cgttggggcg caaaagcaaa ccacacaaga ggagttagtg aaaaatgaac tgactggtgc
    11941 agagttgacc tccctgcaaa aagttcctga tctgatcaat aatttgctga atgaaaaagt
    12001 tgaaggtgtt gtgctggaac aaccagttgc agaagcatat gcccaacaga ataagaaagt
    12061 agctttgact ccagtaaaat ttgcgcaagg cgaaaaaacc acagctattg cgatgtcaaa
    12121 aaatacacca gaactgaaaa atcatgtcaa tgcgtcaatc aaaacgatca atgacgacgg
    12181 cttgctgaaa gattatcaaa aacaagcgaa tcaattgatg ttccaagatg atcagtcctt
    12241 tcttcaaaaa tacggtccgt attatttgat tggaacaaaa tacactattt tattagcttt
    12301 tgtgggtgta ttgtttggaa tggtctttgg tagtttgctt gcactgatga aattgggtaa
    12361 gaataaattt ttaaaggcat tagccattat ttatatcgaa tacgtacggg gaacacctct
    12421 cttagttcaa atctttatcg tttacttcgg aacaggagta ctgggattgg atctatcgaa
    12481 actggtagca ggttgtattg ctgtctcgtt gaatagtgct gcttatgtag ccgaaattgt
    12541 ccgtgccggg atcaatgcgg tcaacaaagg acagatggaa gctgcacgtt ctttaggaat
    12601 ggaccagaaa gaagccatgc gtttcatcat cttcccacaa gcaatcaaaa atattcttcc
    12661 agctctagga aatgagtttg tcacgatcat caaagagtcc tcagttgttt cggtcattgg
    12721 ggtatcggaa ttgatctttc aaacgggcgt ggtacaaggt gctagcttca aaccattcct
    12781 accatatgtg ttggtctctt tgatttattt cgtgttgacc ttcagtattt cccgtatatt
    12841 agctttagtt gaaaggagaa tgggtctgaa tgcttaatgt aaaaaaactg tcaaaaagtt
    12901 ttggcgagaa tgaagtctta aaatcaattg atctttctgt gaaagaagga gaagtagtgg
    12961 tgatcattgg cccttcagga agtgggaaaa gtacaattct acgttgtatg aatctgctgg
    13021 aagaaccaac ttctggtgaa atctttttta agggacaaaa cattacagcc cctgacagcg
    13081 atatcaatca aattcgtcaa aaaatgggaa tggtatttca aagttttaat ttgtttcccc
    13141 atatgaatgt actcgaaaat ttgaccatca cacctgtaaa aatcaaaaaa gaagacccta
    13201 aaaaagcaaa agaacatgct ctatcattat tgaaccaagt tggtctaaag gaaaaagtgg
    13261 acagttatcc taccagttta tctgggggac agcaacaacg ggtagcaatt gctcgagcat
    13321 tagcaatgaa tcctgatgtg atgcttttcg atgaacctac atctgcactt gatcctgaaa
    13381 tggtcggtga agtgttggca gtcatgcagg atttagctaa aaaagggatg acgatggtcg
    13441 tggtcactca tgaaatgggt tttgctaaag aagttgccga tcgtgtgatt tttatggctg
    13501 acggcatcat tcaagaagag ggaacaccga aagagatttt tgaaaatccg caaaatagtc
    13561 gaacacagga tttcttgaac aaagtgttgt aacaaagatg gaggaagtaa gatggacaaa
    13621 agtaagattg cacgccggtt tcagcaacca gcagaaaatc tgttgatgaa tattgctatt
    13681 ttggcaaagg aaaaagagga tattatcgat ttatcgattg gtgatcctga cttgatcact
    13741 gatccgacga ttattgaagc agcttataca gatgtaaaaa atggtgctac taattatact
    13801 gcgcctgatg gcagcaaaga attcttgtca acggtcgtag atttttatca aaagaaatat
    13861 ggattgagcg ttgaatgcaa ccaagttcga gctacagtcg gagcaatgca cgggatgtat
    13921 ctagttttac aggcattact agatccagag gatgaggtca tcatccatga accttatttt
    13981 tcaccatatc gggaacaagt cattttatct ggaggcaagc ctgtttttgt tcctacctat
    14041 gaagaaaagg gcttccagtt ggatgtggaa gatttaaaag cagcgattac ggatcgtaca
    14101 aaggtgatcg tcatcaattc gccgaataat cctacgggag ctgttttttc tgaaagcacg
    14161 ttgaaaaaaa tcgctgaagt cgcaatcgaa aatgaactct atatcatttc cgatgagatt
    14221 tatgaagact ttgcctttaa agagaagttc gtaccgatgg cgcgatttgc gcccgataat
    14281 acgatcactg tcagtggtgt atcgaagggg tttgcgatga ctggctggcg cttagggtac
    14341 atgatcgcac cagcatacat caatcaaatt gctgggttga tcaatgaaaa catcacttat
    14401 tccgcaccga ctgcttctca aagaggtgca atctacgcct taaagcacta tgaacagctt
    14461 gtaccgccag ttgttgaagt attcaaagaa cgattagctt atattgagaa aagagtggca
    14521 gaaatccctt acttatcttt atcttctgtt tctggaagca tgtatgcttt tattaatatc
    14581 agtaagagtg ggctcgattc agtaacgttt gtggaagagg ttttagaaaa aagcggcgta
    14641 ctttttgtcc ctggaaaagc gtttggggaa acgatcgggg acaactatat tcgactagca
    14701 gcgactcagc cattggaaat cttaggtgaa gcctttgatc gtttagcaaa actaaatttt
    14761 tagataaaac tgtatatttg aaaacagcta tgggtatcca ttagaacaaa gagggaaaat
    14821 gatcctttcg agagttggct tgttgactgg gcagcgtcag gatttgacaa tgaaaaagtg
    14881 gtagtggaaa atcaaagtcg attttccact accataaaat attatagact ccattttatt
    14941 gaaccaaaat ttttgaagtc ctcccgcttt tacgtatgcc tatcactggt tactaaactc
    15001 gctaataaga atgttttttt gtcgatctag gatggagaga ggggcaaact ttttcgatct
    15061 acatacagac agtccgattt ttctgacatt tcctattcat ttctagaagg tcagcctatt
    15121 acaacaaact tttttctgtc ctccatcatc ggattttcct gaaggtctac atattggaca
    15181 cgtccgctgg ggcttggtga tcgtttgact tcttccacga attttttgat tgcctcatcg
    15241 tcgcccgcag cttcgatcgt gacagagccg tcatcttcat ttttcactgt tccggtgatc
    15301 cctaattgat ccgctacgat ttttgtcata tagcgaaaac caacgctttg tacacgtcct
    15361 tgcacgttca tcttgatttt gttcatttta ttcaccgttc tttcttttat ttttaaatta
    15421 atgataccga cttttttgct actgaacaag tgcaatgatc taatccatgg tataatcagt
    15481 tgaggtgaaa aatgtgaaag agatccaatc agcaaaaaat ccaagaatca aagaattaaa
    15541 aaaattacat aagaaaaaat atcgtgaaga aaaaggggaa tatctgcttg aagggtttca
    15601 cctgatcgag gaggctgttg cctcaaatgc gaaggtcaac gaacttttta ttacgcaacg
    15661 cggtctgact gagtgggcta gttggcttga agaaaatcag gcgctttctg ttacactagt
    15721 atcagaagaa gtgatggcaa ctgtttcaga tctaccaact ccgcaaggga tcatggcagt
    15781 ggtggaagct gaaaaaagag agataccgaa ttttgaaggt ggttggttgt tgctcgacaa
    15841 cgtacaagat cccggcaatg tagggacaat gatccgcaca gccgatgctt ttggtctatc
    15901 ttgtgttgta ctaggtaagg gaacggcgga tgtctacagt accaaagtat taagaagcat
    15961 gcaggggagt aactatcatt taccgatcat ccaagaggat ctaacaaaac tattgcctat
    16021 ccttaaagag caaggattta taacctatgg aacagaactg aatgaagaag cagtgtccct
    16081 gaatgatatc cagacaacag aaaattatgc gatcgtgatg gggaatgaag gtcaaggcgt
    16141 acgtccagaa gtgctggcgc taacggataa aaatgtgtat ataccgattc aaggaaatgc
    16201 ggagtcttta aatgtaggag ttgcagcggg catcatgctt tatcacttct ccgtatagtt
    16261 aaggaactat taagctttct gcattttgcg aaaacctcac tagaatatta cgaacactag
    16321 tggtatacta agttcaattt caaagataaa tggaattcaa aaaggtggca gatcgataat
    16381 tatgacagaa aaatggcgtg aagacgaaga atatatgtct tatataaatg atctattaga
    16441 aactgatgca gtaaagaaat tagcaaatta cacgcaacat gtccactcga cacgattaga
    16501 gcattcaatc agtgtgtctt actatagcta caaattagcg aaaaaatggg gcggtgatgc
    16561 aagagcgact gctcgagcag gccttttaca tgatttattt tactacgatt ggcgtacgac
    16621 gaagttcgat gaaggtacac atgcgtatgt tcacccaagg atcgctgtga aaaatgctga
    16681 aaaaatcacg gagctttcag atctagagcg tgatattatt ttgaaacata tgtggggagc
    16741 aacgattgcc cctcctaaat ataaagaagg ttatatcgtt acttttgtgg ataaatattg
    16801 tgcagtcaaa gaagcagctc aaccaatgac agcttcaatg aaacaaaaat ggcaacgata
    16861 tttcggtaaa gaacaatcga tttaaggtag ttaactaagt atttgacccg agtattttga
    16921 aaaaaatcct cgggtttttt gtttagacat tgactaattg gtctttttag tgtttacttt
    16981 gttgagatga aaaagtacat aaccaacata aaaataaagg gggtgatgat aagatgcgat
    17041 gtgtatctta tttaaaagag tttgagggct ttatctaaag gcactttgat agaaagatca
    17101 cttttttctt caaagtagcc gattttgaaa gaagaaagga ctcaaagtca tggaaaaaac
    17161 aatcaaacca tatcaatatt attttatcaa gcgagaagtc gagcagctgt taagcgccta
    17221 tagttcagtg aatgatccaa agacagtcca aacagtccaa gcattagctg cggaaaagat
    17281 ccacgaggta cttggtcatc ctaatacagc tgtaaatgaa tttatggaaa aggtactaga
    17341 cgtcactcta aaaaaagaaa aagcagaacg actattagag gaattgaaag tgatcgttct
    17401 tccattcata caaccgacaa aaccgcagat cgataaatta ttccgaaaag tgaaaaagtt
    17461 aaaacaacca gattggaatc aactggattt aagggaacat acttatattg gttggaatga
    17521 tccaggtagc caaaaaaaat ttatgatcta ttatgaagaa gaaaaactaa aagggatcag
    17581 tggaacatta tctcctacaa tcaacaaagg aatttgttcg atttgtcaaa gaacttcaaa
    17641 tgtttcactc tttctttcta ccacaaaaac tggttcagat ggaacataca cgaaaaaagg
    17701 aaattatatt tgtcatgata gtgaccaatg taatcagcag ttggtacaat tagctccttt
    17761 ttacgaattc acagaacgag tgaaacgtga gcggtgattt cattgtattt ttctgaaaaa
    17821 tatgctatct tatttggtga agtgaatagg tgatggtgtt cgcctttaaa cgttgatcgt
    17881 aagataacta atgacgccta ctaaagaaac ggggctaccc ctttttttag taggcgtttt
    17941 tttgaggagg aaatgagatg gaaaatcgat tacctgatcc attgaaggtg atcatctata
    18001 tgatcaaatg gttattgatc acagcaattt taggtatttt tatgggaagt ttatccgcat
    18061 tctttctaac tagtttgact gatgtgacag ctattcggct ggagcatcct tggttacttt
    18121 ttcttttgcc gataagtggg ggactttttg cttatctata tacgacgtat gggggcaatt
    18181 cttcccgggg aaacaattta gtgatcgatc aagggaatgg aggaaatgaa aagattccat
    18241 tacggctgat cccactgacc ttatttggca ccattaccac gcatcttttt ggtggttctg
    18301 ttggtcggga gggtacagcg gtccaaatgg gcggggcact tgctgagaat tttgctcgtt
    18361 ggctaaaatt gaacaaaatc gagcgtgaga ttcttgtcat cagtgggatc agcgcaggtt
    18421 ttagctcagt atttgggaca cccttggctg ggacattgtt tggattagaa gtattggcaa
    18481 tcggaaaagt gagaaccgaa gcactgtttc caagcttttt tgcagcactt tttgcgaact
    18541 ttgtaacgga aagtttcaat gtcaagcata cccattatca aatgggggcg attcctgcat
    18601 gggacccttc acttttcttc aaattgttgc ttgcgggcat tgcatttggt atcattggct
    18661 ggctctttag ccgttcgatc gtctggttaa aagcttggta tgcaaagcgg atcccccatc
    18721 cagttttacg caactttatt ggaggagcag tcgtggtact cgtagtagtg attttacaga
    18781 cgcaaagata tctcggatta agcttacctt tattacaaga tgcatttaat ggaagtgctc
    18841 atccttatga ttttcttggg aaacttggct ttaccgttct atcacttggc gcgggatacc
    18901 aaggaggcga agtgacccct ttgtttgaga ttggggcaac tttaggtgcg acattagcac
    18961 ccattcttca tgtatcagta ccttttttag caggattagg gtttattggg gtgttctctg
    19021 gagcaacgaa tacaccgatt gcttgctttg ttatggggat cgaacttttt ggaggtcaag
    19081 cagccagcta cttctttatg atctgtttga ttagctttat gtgttcagga aatagtggaa
    19141 tctattctgc gcaaaaagtt gaagtgaaaa aaggaatctt gttcctaccg atccttggaa
    19201 gaatctcacg acaaaaagag aaataaaaag aggttgggac agaagtgttt aactccgaga
    19261 aataagaaga aattcacgaa aattgctttt taaatttttg tgaatttcgc ttatttccga
    19321 aggagttgct tctgctccca ccgtttattc ggagttagag tctgtggcaa aactgatttt
    19381 tagttttgtc ccacgctctt ttttattgag caaatttgag aataagagca gaaaatactg
    19441 caaagccaat cattaagcca cagatgataa gatggcgttt tttgtgtaag acaaggatgg
    19501 caataaattc tcttaagata gcattcggta agtagtatag tgcagttttt gcaccgattc
    19561 cgtttgcatt gagtttcgct tgtcgcgcat atagtccagc tctaaagata tggtaattat
    19621 ttgaagtaaa aatcgcgcga taaggcccat tcatttgctg atccattatt tcttttgaaa
    19681 acagcatatt ttctagcgtc gttgtggaat tcgtttctac taaaatatca cgctcgggaa
    19741 tccctttttc taaagcatat tgtttcattg caatggcttc agctactttt tcatcggttc
    19801 cttttcctcc tgacatgagc aagactggcg gattcaaagt tgctttactt tgtgcccaat
    19861 aaaatgcgat cgccttattg atccttttag caagtagggg agatacgcgt tcaccatcaa
    19921 tcaagccagc gccgagaaca acgatataat cttggttata ccttggacgg ttgaactgat
    19981 acaaaattga aacagtcata aaattaaaga aaacgatgag tagataagca aaaataaacg
    20041 gaatgacagc aaatagagca tttgcccatt gtgggagcca atggactaat gtatagtcca
    20101 atatcaaaaa gatagtcaaa gcgactccta acaagagagt caaaaggttg gccaaagaac
    20161 gggattcttt tttcatgact acaacaccat tccaaaataa aaaaatgatc aaaatgtaga
    20221 taccgattgc caaaagaatt aatattggta tcccgatcaa agccaataac ccaccgataa
    20281 agatattttg cgtctctatt agtagccacc ctagataacc accaaagaca atcaaaaacg
    20341 tattaaaaag tacgccattg attaacctac gtttttcctt aaaatagaaa aatgcaaaaa
    20401 tactgaaaaa tagcaatgga atcaaaaaat aaaatgtatg ataaaaaact tctaaaacga
    20461 cgaagtaagt gaccatcatc ataaaagtcc acgtcccaca ataaaagaat agagcgggat
    20521 cgctttggcg cttgcgccaa tagaggatgc ctgacattcc taaaatatag ataactaaaa
    20581 atatcaggtc tctagtaaga ttcattgaat gaactccttt cgatttgtaa attttttctt
    20641 aaaaaagata gcatcattta gtagtaaata tatcgaatat agaaaagtta gttactaaat
    20701 agtcaatcag ttttttcacg acgtttctat tataacgaat tatgttgaaa aaagctttta
    20761 tcgaaaacat aaatcttcaa attaacagat tttttagacc tcgatcatga tgaagacttt
    20821 tgtagataca aataactgta acattgaaaa tgaatgcgaa aacgatattt ttttttattg
    20881 gaaaatatcg gtcaagcaat aaaccagttg cataataaaa tgttaacggc aacaaaatct
    20941 gtggaaggaa tgaaccggat gatttgatgg aaaaaacaag atagacaaga agtatacata
    21001 gtaaaacgtg gaacaagtag gccagaatga aaaatcagtt tttatccact cgcttttcta
    21061 tacattcact gtcacaaata taatcgaaag taattctatt ttgagataaa gtattttata
    21121 ggagatagta tatgaacaaa aaaagattta aacgtattga aagaaaattt gcattaactt
    21181 atgaccaagc taagtatttg aaaacactat ttccaaaaca ggtagttcgg gataaatata
    21241 gtgatcattt tatttcttcc ttgtattttg atacaaacga tcatcacttt ttaaaagaaa
    21301 tcactacaga aaagtcctat cgagaaaaat ttagaatcag aaaatatgga gatcctcagg
    21361 acatatcgcc tatctactta gagataaaaa gaaagtttca aggaatgaca gataaacgta
    21421 gaataaaatt agcgcatcat gagctcgatg atatgttgga aaatatcgaa ttggcagaaa
    21481 ccacattcag taatttagaa gaagagtata tttttaatga aatacagtgg ttgttcagaa
    21541 ggttggcatt ggagcccaaa gtatatatct attatttaag agaagcattt gtaaatagag
    21601 attcagaaga tttgcgaatc acttttgatc aaaatatcca ttaccgtcta atgaacttaa
    21661 cgacctggga acactgtaca gaagacaatc tggtagcgcc agaaatcgat gtgctgatgg
    21721 aagtaaaaac gacaggagaa atacctgaat ggttacaggt aggattggat aggatgggta
    21781 aaaaagagat aaaattttct aaatataaac aagtctataa aagatatatc caagaggacc
    21841 cgctctatgt tcaatagtgt cttaaaaaat gagccttctc aactcacact agatgatttc
    21901 tttttatgta tggtggtgtc gattttgcta ggcattctga ttgcttacac ccatatgtat
    21961 caaaacaact gtacaaaaaa cttcatgttt gccttgattg ggttacctac tttagtacat
    22021 accgtcattt tgttgatcaa cggtaactta ggcacgggga tcgctgttct aggcgcattt
    22081 aacttagtgc gatttcggtc tgctcaaggg aattcaagag atattttcag tattttttgg
    22141 gcaacgtcga ttggtttagc cacagggacg ggatatattg tctatgcact attcgtgtct
    22201 agtatagttg ctgctatcca agtcatcatc ttcacgcttc caatcaaaaa taaacataca
    22261 gtttttaata ctgaaaaaac gatcaagcta aaagtttcta aatccttgtt tctttcgagt
    22321 attattgaag aaaaaattgc agaatataca gaaaactgca atttaaaaaa agtaaaaatc
    22381 aataaagaaa cttgttcctt acactatata gtaaaagtaa aaacgagtat gcttgaaaaa
    22441 gaattggttc gaacgatcat ggatcatcca gaagtcattt cgatcactgt aggtatggat
    22501 aaatcgaata aattattgtg ataaccaaga aagtacttaa caatgaagta agaccccagt
    22561 tccaaagttt agcggggaca cttcaataaa gtactttctt ttttgtcttc agcaactact
    22621 ggtaaaactg agagcaagct ttcgtgagag tacagcgata aagatcaacc tcccttttta
    22681 tctcgagcaa tttccttgca aggctgtttc gttgatctta aacgagttag caacagtcaa
    22741 agatgcgatg gccataaaaa aattgcatta agttggctgg tatctgatag ctgggaatct
    22801 aaggaagact atccattctg tttcttaaat tccgctagtt gatagatatc agccaattgt
    22861 tccagatgat caacatcaaa gataaccgaa agatgtttcc ttttattccc atttgcttga
    22921 agagttttta tcttctcttt ggttccttca ctcaatgaga gtgtgactaa tttctccctc
    22981 gttttttctg ggaaatgaaa ggatactttg aaaaatccgt cccaaataga aagccagaag
    23041 atcggtttta ctttctgaga gtgggacaaa agtaagaaac acttttgtct cattctttaa
    23101 aaacgaataa acggtgggag cagaagcaac tccttcggaa ataagccaaa attcacaaaa
    23161 atttgaaaag caattttcgt gaatttcttc ttatttctcg gagttaaaca cttctgtccc
    23221 aacctcttgt attcaccttt tgatagccaa gcttttccat cattataaaa ccgcaaattt
    23281 caaaaaaaaa cgaacaatac aaaaagcgta ttgctcgttt ttctcttcag tttctcacac
    23341 ctaaacgact tttgtctcga cctctttttg gtgactattc atgatgttaa aataaattac
    23401 tgaaaaaatc tgtgacatgg cgccaaccaa tgacaaaaat attagctttt tcaacagtag
    23461 aagtggtaat gatgtctgtc gaaggctctt ggtttgcttc caaatagcct aatttgtcat
    23521 cagctaattg gatcgttgct gagccaactg tagtgccttt gttgatagga gcggtcactt
    23581 ggtcatccgt tactttcttt ttatcaattg ttggagtaat cgttaatttt ccagtatcca
    23641 ttccattttc gacccagact tttacagggc tttttagcgc aacattgacg gaggtttctt
    23701 ttccatcttt cactttgatg tcttttaggc ttgggatgct tgcatttgct tttccaagtt
    23761 ctttttctga ccagttgtca taacaataat ccatcaatcg tgatgtttca ttgaatcggg
    23821 cacttggatt ttcttcatgg ttatctgcat ttaaaacgac tgtgatgatt cgtcggccat
    23881 ctttgttgat cgttccgaca aagcatgcac ccgcaaaatc agttgtgcca gtttttaatc
    23941 catcgacacc ttcgtgatag tttacaaaac caggtagcat ccagttccaa ttgaccattt
    24001 caaccggtga ctgtgtgttt tcgccgaatg tttgatttgt ggtactcgtg atttttaaga
    24061 cttcaggata atctaagatg aggtgacgag caacaatagc gacatctttc gcagacagtt
    24121 gattttcatc tgtttcacca gtattctcag gtcgattctc acctaggtaa gaattgttta
    24181 aacctgatgc attgacgatc gttgcatctt tgatacccca atcttttagt tgtttcttca
    24241 ttagctcaag aaatttactt tcactgcctg caactttttc agctaaagca accatggaag
    24301 cattggctga ttggatgaaa gctgagtcaa caagttcttt cactgtataa tcattttctt
    24361 tatgcaaagg aacattcgat agatccggca cgacacttaa tttttctgca taatccgaaa
    24421 tggctacctt atcttcccaa gataatttac cctctttgat ttgttcaagt acgatatata
    24481 gaccaatgat ctttgtgatg gaagcaattc ccatcgtttt ttcgccatcc tgatcataaa
    24541 ggatttttcc actttctgca tctacggcaa aggcagcttt ggcatttatt ttaaatgcgt
    24601 cgtctgcttg aacagttgcg ctaggcaaga aagaagtgat acataagata agacttgcta
    24661 atagtacaat aaaacgggta ttttttttga acatctttgt tctcctcatt tttaaattga
    24721 ttcttacaac ttagaatatt atcagagaga acaaaaggtt tcaatggttc tgaaaaaaac
    24781 cttataaaga acaagtctta ttaatgaaga atcatagaaa gttatctttt gggttcattg
    24841 acacgaactt tgactggctg aacttcgggt tcggttaggc gtgatgctgc aaaaagggcg
    24901 cctgctgaga gtgcgaccaa actaaaaatc atgaataatt gcataagatc acctcgttct
    24961 tttttgtttt tttatagtat agcaagagag tttgaagcta ctgttaagga aatgagcata
    25021 taattgatga aagtatgaaa aaaatatgaa gtaggacaac acaactaaaa atcgatcagg
    25081 tatttctctg gtagggattc gttttattgt attgataaaa tgggatgtgg tagactttat
    25141 ttacagatac aaagaaatga tttttttagg aggggtacgt ttatggcaaa aaaagaaaaa
    25201 gaattattat cacaattgac ttcagaaggt accaaaggac cgtttgtcac gattatgtta
    25261 aacacccatg ttgcgcatca agacgtagaa aaagatcaaa tcaaattcaa gaattttgca
    25321 aaggaagcga aaaagcgctt tgaaaagaaa tactctgagc atgattggtc tccattccaa
    25381 gaaaaaatta ctagtttgtt ggacgatccg tctttctggc gtagtgccac aacgagtgta
    25441 gctgtcatag tatcaccaga acaagtgttt atccaccgtt tgggtattcc tgtagatgac
    25501 cagtattatg tgagtgatct accttattta ttagcgatca tcaaaaatgg acatttcaac
    25561 tattcatact atttattagg attgaatcgt gattcgatga aattgtacga agtaacgaat
    25621 aaacaagtca aagaagtggc tttgcctgaa gatgcaccaa gagatgtcgt cactgcttta
    25681 ggatctgaac taacaggagg cgggttgaac tacgcatcaa atggtagtga aggtacgacg
    25741 ttccatggag tcaatcctaa agatgaagaa gtcgaaattg attgggtcaa ttattatcaa
    25801 gcagtcgatg actatttgaa agatacattt gaaaatccag agaaatggcc tatcttctta
    25861 tttgcattac cagaaaatca gacgatgttc aaaaaagtgg cgaagaaccc ttattatcgt
    25921 tccgatgttg cgatctcagc ttcacctgca caactttcat taaaagaaat tgaagcaggc
    25981 gcaaaaaaac tatcgcaaga attagctgtc aaagaagcaa gtgactacaa taagttgctt
    26041 gataaaaagt tcattgatca actcgttgat attataccgg cagcaaaaga agggaaaata
    26101 tcccatttat tcattgctac atctaattta gcagatggtt ttggcgaaga tccagaaaca
    26161 gaatatgacc gccgtcaggt gctgaatgaa ctagcagata atgtgatcac gaacggtggt
    26221 caggtcttta tccttgaaca aaaagatgca ccagacgaaa agagtttagt tgcgatctta
    26281 cgttattgag tgaaaactac caaagtagat gaatggtcag gcgttgatag aagtgtatag
    26341 ctccgagaga aggagtttcc tctgtttcag ccgttggatt catcttgagc gagagacaaa
    26401 agctagcttt tgtctctcgc tcattatttt tgtgctttag tcccattggc tttgcgaggg
    26461 gaagatactt ttctcaaaaa aacgaacctt gcagttgaga gggggataga tgcgcatact
    26521 aatttttatc agttaaaaaa tgatgtggat cattagagaa ttccaaagtt cgttcatcct
    26581 ttttcagata ataaattgaa attttttgca aaagtgaaaa tagcaaatag aaattttctg
    26641 aatcttatag gggtgatttc gagaaaacgt tcgtttattt gcaaatatat cttctaaata
    26701 agagaataaa tacaaaaaaa taaggaaaaa ctctggcaat tttgtaaacg gtatgatata
    26761 gtaagaatgt agtttttgtt cggtaatctg tggtagcttg tttcaaataa acatcttcca
    26821 gatatacacc attcaagtaa ttgcaaaata tgtgttgata cacaaggagg atttttatta
    26881 tggaaacagg tacagtaaaa tggtttaact cagaaaaagg atttggattc atcactgcag
    26941 aaaacggaaa cgatgtattc gttcatttct cagctatcca aggcgacgga ttcaaaactt
    27001 tagaagaagg ccaagcagta actttcgacg ttgaagaagg tcaacgcgga cctcaagcaa
    27061 caaacgttac taaagcttaa tttacctata ttaaaaataa aagtccagac atttatgtgt
    27121 ctgggctttt ttgtcttttt ttccttaaaa agcgatgaga gtatttttct gcactttgta
    27181 gtataatttc aaaagaacat tagaggcagg agaagaggaa gatattttga aaccaagaac
    27241 ttatgcagtc gttgatatcg agacgactgg gacgaatcca aaagaagatc ggatcatcca
    27301 atttggctgc gtcatgattg aagctggtcg gatcacttca cgtttttcga tcgatattca
    27361 tccagggcgg aagatttcaa aacaaatcca gcatttaaca gggatcacga atcaacgcgt
    27421 ccaaaaagcg ccttattttg aagatgtcgc ccaaacgatt tacaatcttt tggcagacac
    27481 catatttgtc gcgcataata tttattttga ttataatttt ttgaaccatg aactcatgcg
    27541 atgcggactt cctagtttga agataccggg catcgatacc gtagaacttg cacaaatttt
    27601 cttgccgacg gaaccaagtt ttcgcttagc tgatttgtca gaaagccttg gctttattca
    27661 tgagaatcct catcaggcgg atagtgatgc cgaagtgaca ggtcagcttc ttttactgat
    27721 cgaagaacga atgaggaaat tgccgatcat cacattagag cagattgcac gattaagtag
    27781 ccatacagga atggatacga gtcgtttcat ttaccacgtg cttgaagaaa tgaaagaaaa
    27841 cccggagccc cttgatctct cattagaaat catcgatggg ctagctttgc agaaaaaaga
    27901 agtggagcta ttcacatccg tccattatgg cgagcggaca tatccaagaa aaaaacaagc
    27961 aaaagaaaaa atgtttggta aaacactgat gtatcgaaaa gaacagaaca ggctgatgaa
    28021 tgctgtctac gaccatttca caaaagatga atcaaaagat ttgatgatcg aagcagctac
    28081 aggaatgggc aaaacgattg gttatctgtt gcctttgagt tatttggcta cacctgaaaa
    28141 accagctgtc atcagcaccg tttcgcttgt tttgcagcag cagatcatgg aaaaagacat
    28201 tccattactg aatcaattgc tagatcagcc gatacaacct gtgattatca aaagctatcg
    28261 ccactatatt gatttacagc gttttaaagg tacacttgtg gaaccgccgg gacaaaaaca
    28321 atatgctttg taccaaatgg ctgtcttagt ttggctaaca caaacgaaga ccggtgattt
    28381 ggatgaatta catttaacca atctgaacca tgcattcttt gcagatgtcg cgcacagagg
    28441 cactgggttt ttagcaagga atcagtcatt ttacaaacaa gactttgtgc gtttcttgca
    28501 aaagaaaatc aaacaaagca attttttgat agtcaaccat gcatttttag tacaagaaac
    28561 acagcgaaaa gaaccattgc ttccagctag tccttatttg ttgattgatg aagtgcatca
    28621 tttgccggaa attgctgaaa aagtaaatga caaacgacta agtattaact acttccataa
    28681 acaaattcag caactggaag agccgaatca gttatttgac cgcatacaaa gttatttgcc
    28741 gaaggaacat gaagccttgc gctggttgtc gatttatcga gaagaactct ctgcactatg
    28801 cgaagcgcag atttgcgtgg cagagggatt aatgaactta gtaagcagca agcaacctaa
    28861 cggacaatat ccagaagaag tcatgatgac aaaagaaatg ttggatcagc tgccactgga
    28921 atctggaaca gcaatggatc atctgcaact tttttatcaa gagatccaac aactacaatc
    28981 acagatgcgc ttgacttttg agattatcca agaaagttgg accaaccaga tgcggatcga
    29041 tttagcaagt ttatatagct tatttgatca attagacaag caatacttgc tgatggacca
    29101 atggctagaa gactggcgag atcatttgat ccattggttc gttcccggga atacagcaca
    29161 gcagatgatt tttcatctcc ttgattttca agcagctatg ttgccgtcaa ctgtgtggta
    29221 tcctcgatat gaccatattc tttatattgg aggaacactg aagatcggac ctaatcgaca
    29281 gtatctctct aaacgacttg gcattgaagc agcacctctt aaagtgattg cttctcctta
    29341 tgattatgag aaacaggcaa gaatctatgt gccgaaagaa gcacctgcag tccatcaagt
    29401 cagcaatcaa gcatatatcg attatttaga agcagcactc acaggattga ttgaacaaga
    29461 agatcggccg atccttgttt tattcacttc tcatgatgtt ttgcaaaagg tatatcagaa
    29521 aatgcatttg tctatgctag aaaaaggacg tgaaattctt gctcaaggat ttggcgggag
    29581 tcgggaaaaa cttttgaaac gcttcatttt gtcagaaaaa agcgtcttat ttggtgctga
    29641 cagcttttgg gaaggcgtag atttacctgg agatgctttg cagattgttg tagtcacacg
    29701 tttacccttc gacaacccgt tacgtccgat ggtcaaagct aggaatgctt atctggaatc
    29761 agaggggatc aatccttttt atcaggaatc cgtgcctaaa gcagctttga agttacgcca
    29821 agcattaggt cgtttgataa ggacggagac agatcgtggc gtattactta ttttggatcg
    29881 gagattagtc actgcaaaat atggtgcacg aatcatccat gcattaccga aaaaagtacc
    29941 gataaaagag ctagctatag atgaaatcta tcaggatatc catgaattct taaagaaaaa
    30001 tgaagaagga agttaaaggt gccgtctttt ttcatcatta aaatctgcta taatgaaaag
    30061 gaatgaagat aaaggagcga cgacccttga agaaaaaaca gacaaaggaa aaaacgatta
    30121 atcgcgtgtt gcttggcttg atcgtatttt tattagcggt catcgtgatc agctcgattt
    30181 tttacttgcg ttccagtcgt ccggcggtcc aggcgaaaaa agaagcagta gaaatcgcaa
    30241 agaaatatgc aaatctcgag gaagccgatc atttttactg gtttactcga aagaaaacgt
    30301 acttttcatt gacgggtacg aatgataaag gaaaagaaat cgccgttatc attcctaaat
    30361 ctggtgaaaa agtaaaagta ttggatcaaa caaagggact gactgaaaac gaagcaaaac
    30421 agacaattgc tgcggcccat cctgaaatcc aaatcgaaaa agctgcttta gggatgtatg
    30481 atgatcagcc tgtgtgggaa gtagtaggaa aagaaacaaa tgggaaactg aattattacc
    30541 tgattggctt tgaatctgga gatgaagtca agacaatcaa gggaatttaa aaggtaaagg
    30601 aatgaatggg atggagttat caaaaagaac aaaacgatta gaaccgtctg tcactttggc
    30661 agctgcggca aaggccaagg cattaaaagc aaaaggcaga gatgtgctga gtttgactgt
    30721 gggagaaccg gattttgcga caccagaaaa catccaagaa gcagcaatcg aagcaatccg
    30781 caatgggaaa gctagctatt atacaccaac agcaggaatc ccagagcttc gacaggcaat
    30841 cgttgattat ctgaagaaaa actatggact tgtctatgaa ccttcacaaa cagtcgtaac
    30901 agacggagcg aaatttgctt tgtataccct tttccaaacg atccttgacc cgcaagatga
    30961 agtcatcatt cctgtgcctt actgggtcag ttacggtgag caagtaaaac tggcagaagg
    31021 acttcctgtc tttgtgaaag gggaagaatc caatagctgg aaagttactg ttgagcagct
    31081 agaacaagca cgttcagaaa aaacaaaagc cgtaatcatc aattctccat cgaatccgac
    31141 tggaatgatt tatagcaaga atgaactgca ggcaatcggt gaatgggcag taaaaaatga
    31201 tattttgata gttgctgatg atatttatgg ccgtttagtt tataatggca atgagtttac
    31261 tccaattgca acgatttcag aagcaatcaa aaatcagacc atcatcatca atggcgtgtc
    31321 taaaacatat gcgatgacag gctggcgcat tggttatgcg gtggggaatt ctgagatcat
    31381 caatggaatg attgctgttg cttcccagtc aaccagtaat ccgacagcag tcagtcaata
    31441 tgcagctgtt gaagcattga aaggcgagca agatacagtg gaagaaatgc ggaacgcctt
    31501 tgaagaacgc ctgaatacgt tatatccgct tgtggcagaa ctgccgggtg tttctcttga
    31561 aaagccacaa ggagcttttt atctttttcc taatgtaaaa gaaacgctac gcatgtgtaa
    31621 atatgaaaat gtcacacaat gggtagaaga tcttttagaa gaaacagggg tagcgcttgt
    31681 gactggcgaa ggctttgggg caccagaaaa tgtccgcatg agttatgcca ctgatcgcgc
    31741 tacgttagaa gaagcggtac gcagaatcaa gcaatttatt gagagtaaaa gtcaaaatta
    31801 atcctgaaga aattcaagga atataaaagg agagtcatac gtggaacaaa ttcaaatcat
    31861 cgattcgaaa aaccatgtgg gagaaacagt aacaatcggc gcttgggtcg caaataagcg
    31921 atcaagcgga aaaatcgcat ttttgcaatt aagagacgga acagcctttt tccaaggtgt
    31981 agttgtaaaa agcgaagtgc cggaagaagt tttccaaaca gctaaaaatc tacaccaaga
    32041 aacatccgtg ttagtaactg gagaaatccg tgaagacagt cgttcaaaat ttggttatga
    32101 aatcggaatt acaaatgtgg aagtggtaag cgagagccac gattatccga tcacaccaaa
    32161 agaacacgga acagacttct tgatggatca tcgtcattta tggctgcgtt cttcaagaca
    32221 acatgcgatt atgcagatca gaaatgaaat cattcgtgca acgtatgaat tctttaatca
    32281 acatcatttt gtcaaaatcg atccgccgat cttgactggt tctgcaccag aaggcactac
    32341 ggatctattc gaaacaaatt actttgatca aaaagcttat ttatctcaaa gtggccaatt
    32401 atacatggaa gctgctgcta tggctttcgg aaaagtcttt tcattcggtc cgactttccg
    32461 tgctgaaaaa tcaaaaacac gacgtcattt gatcgaattc tggatgatcg aaccagaaat
    32521 ggctttcact catcaagaag aaagtttaga agtacaggaa caatatgtag cattcttagt
    32581 acaaaatgtt ttagatcatt gcgaatacgc attagatgta ctaggacgag acaaagaagt
    32641 cttaaaaact tatacaaaat tgccattccc tcgtattact tatgatgatg cgatcgaatt
    32701 actgaagaaa aatggatttg atgacatcga atggggcgaa gactttggtt cacctcatga
    32761 aacgttcatt gcgaattcat tcgaacagcc tgtctttatc ctaaactatc caaaagccat
    32821 caagccattc tacatgaaac cacatccaac aagagaagac gtggtgatct gtgcagatat
    32881 gatcgcacca gaaggttacg gtgaaattat tggtggaagc gaacgggcaa cagactatga
    32941 tttcttgcta gaacaaatcc gtaatcacgg tttggatgaa aaagaatatt catggtatct
    33001 tgatctacgt aaatacggtt ctgttccgca ctctggtttt ggtctaggcc ttgaacgaac
    33061 agtcacttgg attgctggta tcgaccatgt gcgtgaagct attccattcc cacgtttgtt
    33121 gaaccgaatt tatccataag aggttgtgaa atcagtgcct tgacctgagt attagagaaa
    33181 caaaacggaa aaatagcttc tcatatttta ctgttttttg agctaatatc gagggtcagc
    33241 tgattgaaca ccgtttatat caagaggttg tgcgtctgcc gttcagctcc gagtcacaag
    33301 gagtaatttc aaaaaaacat tttttcatgt ttgatgacta ttgtgacttg tgaccaagga
    33361 gttggccgga gagcaacgtt tatttaaaaa aaagaactgc gaactctcgc agttcttttt
    33421 ttttaaatac gggataaagg aagtttccag cagataagag aagaaagcaa aagaaattgc
    33481 tatttgattc acaaggttct ataacgaaat cgcaatcaat caaacatata caaagaaggt
    33541 tattcaaatg gaaatctgat ttgatgatgc ttctgctgtt gaaataaagc agcatctagc
    33601 acctgaaaac aaactaatag taacattcga agatggagta ggtccgtatt ttcagcatgc
    33661 aatgatccat atgtaagttc aattcaccct tgaaatcatc ggaccagata aagttatggc
    33721 caatctatcc caagcaatct agaagagata ttagtaaagg aacatttact ggaagtctta
    33781 gatgagccta taaatatatg ttctgatggc gtcatcaagt ggtctgattg acggaagctt
    33841 aggtgtgatc gatctctctg gaccaaacaa tgaaggaatc aaggggaatc cagcacgtta
    33901 agaaaaaaat agcataaaaa actagacact tgatgagcgt tcattcaatt gtttgaaggg
    33961 actaggctgg tgtcattttt tatacatata ttttctcttt cgtcttgata gggcttatgg
    34021 gataatataa ggaagaaaat catcgtttta ttcaaaaagg agtttttgac aaatgaaaaa
    34081 acgtatatta tttatcggtg acagcattac agacgcagga agagaccgag cagaagcaac
    34141 tgatctaggt aaaggttatc cacttttgat tgccggaaaa ctaatctccc aatatccaac
    34201 gatggaattt gaatgcatga accgtgggat aggaggagac aaaattgcag atctgcaagt
    34261 acgctggcga gaagattgtt tagctttaca acctgactat gtatcgattt tgatcggaat
    34321 caatgatacc tggcataata ctagcgatca ggctatattc ggaactgaga aatctgctca
    34381 gcaatttgaa tccgtttatc gaaaattact aaatgaactt caaaaagcgg gaatctgcaa
    34441 catcatcttg atggagccgt ttgtacttcc tataaccgac gaccgtcatg gctggcgtaa
    34501 agacttagac cctaaaatcc aaattgttcg taaacttgct aaggaatatg gcgcattgct
    34561 ggttccttta gatggcatat tcaatgcttt agggattgct tatggttttt cgaaatatac
    34621 aagagaagat ggggtccacc caacgattgg cggacatgag atcattgcac aaagctggat
    34681 agatgcaatt gccgcagtcg atttttttag tggagataaa aatgagcgaa agaaaagtga
    34741 gaaatgatgg aaatacggtg ggcagtaaaa gaagatctac ctcaaatcaa tcagctgatt
    34801 actgatcatc cagaatcatt cgtaaaagca aaaacggcag ctgacctaaa tgataagaat
    34861 aaactattgc tcgttgctgt aacaaaagaa aaacggatta tcggttttgc gcaagcggac
    34921 gtgcaacagt cagaagaacg ggtgggtcag cgaccatcag gaaaactaat ccaacacaca
    34981 ttagtaacaa aagaaaatac ggaagaaatc caaaaaaaat ttgcagaata tttgatgctg
    35041 gaagctaaaa aaagaagaat aaaatcaatt tttgcccagt tttaatcaaa aacagtaata
    35101 gaaggagaaa tacaatgagt tgtttaggaa atgtcatctg gtttatattc ggcggactaa
    35161 tcggtggtct tagctggtta gcagcgggga tactttggtg tatcacgatc ataggtatcc
    35221 caatcggtat gcaatgtttt aagatcgcct tcctttcttt tgcgcctttt ggcaaagaag
    35281 taatttatca ccagtctggc atgtcattga tcgttaatat catctggctg atcgtttcag
    35341 gcctaccaat tgctctcgca catgtcgtca gcggcatcct tctatgtatc acgatcatag
    35401 ggataccttt tgccaagcaa tctttcaaac ttgctcgatt ggcgttgatg ccattcggtg
    35461 cagaaattgt ataaaagaga ataataaagg ttagataaag catagagtca ggtgggatac
    35521 atgtatttca cctgactcta tgcgttttcc tgtataagtt cttccatgtg acttttacgt
    35581 cttttattta tatggggtta ctgtttatgg aaagttgaaa taattttcat taaagaactc
    35641 tttctatatt tttgaaagcg tagtggagct tcgtatatct gggaaggaca ttttgcaggg
    35701 attgaaaaaa gatattgtcc tatcgaaaaa ttaatgaatt tagacaaatt gccagcaaca
    35761 ggcttcaaac ttagttgcta tcctgtcaag atcaagaagg caagtgcggg ttggataaga
    35821 gcagtggcaa tgatcgaaga gaaaaagaaa gaataagcca aaacatctgt atggaagaaa
    35881 ttttccgatg cagatgtttt tcattttttc tttcggatac cttgacatat gaataaccat
    35941 tcatttataa ttagttatga agagatattc atatgttgag gcgaggaggt cattaaaatg
    36001 aaaagttaca gattagaagg tttagattgt gcgaactgtg caatgaaaat cgaaaaagga
    36061 gttcagaaaa tcaatggagt caaggaagca acggtaaact tcacttctgg aaaactaacg
    36121 attgacgcgg aggaagatca tttggctacg attgaacaag aaacaaaaaa agtagtgaag
    36181 gaattagaac cagatgtcaa agtaacagaa atagataagg aaaaagtatc tgaacatgga
    36241 aatgaaaaag agagaaatac gctgttccgg attttatttt cccttgcagg tatcgcttta
    36301 ttgctattat ttgattttaa tgaactaatt cgcttgatag ggtatttatt gatttatctg
    36361 ctgataggat atgatgtagt gaagaaggca gtgatgaata tcgtcaaagg aaaaatcttt
    36421 gatgagaatt ttcttatgtc tgttgctacg attggcgcta tgttgatcgg tgaatatcca
    36481 gaagccgtag cagtgatgct attttatcaa atcggcgagt atttccaagg attggcagtc
    36541 agtcattcaa gaaaatcgat tcgcgaattg atggcgatcc gtccagaaac agctcacgtg
    36601 cagacggcgg aaggattgat gaccgtgaac cctgaagacg tgctgatcgg tcagtttgta
    36661 cttgtgaagc ctggtgaacg tgtaccgtta gatggaacga tcatcgaagg agaatcatta
    36721 gtcgatacgt cagcacttac aggagaatca gtccctaaat ctgtatatgt tggtgaaaca
    36781 gtattaagcg gatttatcaa taaaaataaa ccatttttag ttcaagtaga aaaatcttat
    36841 gaaaattcta ctatcagcaa attattagaa ttagtagaaa atgccagcag taaaaaagca
    36901 ccagctgaaa atttcattac taaattcgcg cgctattaca caccagtcgt tgtaggattg
    36961 gccgttttat tagctgtttt acctccactt gttgtttctg gagcggcgtt ttctgaatgg
    37021 atctatcgag cattgacatt tttagtgatc tcatgtccat gtgcattagt gatttccgtg
    37081 ccacttagtt tctttggcgg tatcggagga gcaagtaaaa tcggtgtatt agtaaaaggt
    37141 tcaaattact tggagttgct tgcgcaaacc gagacagtgg tattcgataa aaccggcaca
    37201 ttaacgaaag gcgacttttc gatccagacg atcgatacaa aaatcgatcc aaagatattc
    37261 atgcaatata ttgcgagtgc agaacagttt tctacacatc cgattgctca atctgtttta
    37321 gaaggctacg caggtccgct tttaccaaca gctaatatcc aggaatttgc aggtgaaggg
    37381 atcttagctg aagttgatgg caaacaagta ttagtaggta atcacaaatt gatggaaaga
    37441 tttgaaatct cttttccttc aagtcaagaa atcggtacat tgctttatct agcaatcgat
    37501 caaagttaca gtggttatct tgttattgca gataccttga aagaagatgc cgtagacgca
    37561 ttggttcaat taaaacaggc tggtgtgaaa aacacagtca tgttgacggg cgactcgaaa
    37621 aaaattgctg atcatatcgg gaagcaagta ggcgtggaca agatatatag cgaattgctt
    37681 ccagaagata aagtgcagcg gttagaggaa atcttacaaa gtaacaataa aaagaccgca
    37741 tttgtcggag atggaatcaa cgatgcaccg gtgctagcaa gagcagatgt aggaatcgca
    37801 atgggtggat tgggaagcga tgctgcaatt gaagcagcag atgtagtcat catgaatgat
    37861 caaccatcga agattgcaga agcaatccat ttagcaaaga aaacattgaa aatcgtcaaa
    37921 caaaacattg tctttgctat tgggataaaa attttggtac tttccttagg tgcttttggt
    37981 tttgcatcaa tgggagatgc agtatttgca gatgttgggg tgacagtatt agctgttttg
    38041 aatgccatgc gaagcttgca tgtaaaaaat aaacgttgat caatgaagag gac
//
LOCUS       DAZBSA010000016        34663 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000016 DAZBSA010000000
VERSION     DAZBSA010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.016"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            591..1280
                     /locus_tag="ACHGMT_000743"
     CDS             591..1280
                     /locus_tag="ACHGMT_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN6834512.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="ACHGMT_000744"
     CDS             1267..2475
                     /locus_tag="ACHGMT_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFN6834513.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="ACHGMT_000745"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="ACHGMT_000745"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFN6834514.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACHGMT_000746"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACHGMT_000746"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFN6834515.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="ACHGMT_000747"
     CDS             complement(6578..7273)
                     /locus_tag="ACHGMT_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFN6834516.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACHGMT_000748"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACHGMT_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFN6834517.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="ACHGMT_000749"
     CDS             complement(8472..8663)
                     /locus_tag="ACHGMT_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFN6834518.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="ACHGMT_000750"
     CDS             complement(8684..9574)
                     /locus_tag="ACHGMT_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFN6834519.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="ACHGMT_000751"
     CDS             complement(9561..10328)
                     /locus_tag="ACHGMT_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFN6834520.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACHGMT_000752"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACHGMT_000752"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFN6834521.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACHGMT_000753"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACHGMT_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFN6834522.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACHGMT_000754"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACHGMT_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFN6834523.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="ACHGMT_000755"
     CDS             14899..15282
                     /locus_tag="ACHGMT_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834524.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACHGMT_000756"
     CDS             complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACHGMT_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFN6834525.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="ACHGMT_000757"
     CDS             complement(16226..17044)
                     /locus_tag="ACHGMT_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFN6834526.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACHGMT_000758"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACHGMT_000758"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFN6834527.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACHGMT_000759"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACHGMT_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFN6834528.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="ACHGMT_000760"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="ACHGMT_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFN6834529.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="ACHGMT_000761"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="ACHGMT_000761"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFN6834530.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="ACHGMT_000762"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="ACHGMT_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFN6834531.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="ACHGMT_000763"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="ACHGMT_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFN6834532.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="ACHGMT_000764"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="ACHGMT_000764"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFN6834533.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="ACHGMT_000765"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="ACHGMT_000765"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFN6834534.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /gene="rpsF"
                     /locus_tag="ACHGMT_000766"
     CDS             27086..27385
                     /gene="rpsF"
                     /locus_tag="ACHGMT_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFN6834535.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="ACHGMT_000767"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="ACHGMT_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFN6834536.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /gene="rpsR"
                     /locus_tag="ACHGMT_000768"
     CDS             27973..28209
                     /gene="rpsR"
                     /locus_tag="ACHGMT_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFN6834537.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="ACHGMT_000769"
     CDS             28352..30325
                     /locus_tag="ACHGMT_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFN6834538.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="ACHGMT_000770"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="ACHGMT_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFN6834539.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="ACHGMT_000771"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="ACHGMT_000771"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFN6834540.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="ACHGMT_000772"
     CDS             33204..34496
                     /locus_tag="ACHGMT_000772"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFN6834541.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10566 a         6735 c         6853 g        10509 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt actgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tgtattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagat caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgtaaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggatcaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggtggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccggc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagacatt gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atggggaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatcc
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaatcct tgaaaactat
    25441 atcgaacatc aacgcgaagt catcacacga cggacggtct ttgaaaaaga aaaagcagaa
    25501 gcaagagcac acatcttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaggcaaaag caacaatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgtacagc caaaggaatc ccaatcatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggtcagagg gatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aaaatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaataa ccatcaagac taagtgtgta agctagcaag
    26881 aataaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataattag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattagatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaat gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atattgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaagagg tagtgaaaga gacgtttagc cttattgccg ataggctgtc
    34621 tcttgaacac tgtttattca gaataaggga ctgtgacaaa aac
//
LOCUS       DAZBSA010000017        33266 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000017 DAZBSA010000000
VERSION     DAZBSA010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.017"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(42..230)
                     /gene="rpmB"
                     /locus_tag="ACHGMT_000773"
     CDS             complement(42..230)
                     /gene="rpmB"
                     /locus_tag="ACHGMT_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFN6834542.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            500..862
                     /locus_tag="ACHGMT_000774"
     CDS             500..862
                     /locus_tag="ACHGMT_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFN6834543.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            914..2596
                     /locus_tag="ACHGMT_000775"
     CDS             914..2596
                     /locus_tag="ACHGMT_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFN6834544.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2713..4749
                     /gene="recG"
                     /locus_tag="ACHGMT_000776"
     CDS             2713..4749
                     /gene="recG"
                     /locus_tag="ACHGMT_000776"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFN6834545.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEGSEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4798..5799
                     /gene="plsX"
                     /locus_tag="ACHGMT_000777"
     CDS             4798..5799
                     /gene="plsX"
                     /locus_tag="ACHGMT_000777"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFN6834546.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5921..6163
                     /gene="acpP"
                     /locus_tag="ACHGMT_000778"
     CDS             5921..6163
                     /gene="acpP"
                     /locus_tag="ACHGMT_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFN6834547.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            6431..7435
                     /locus_tag="ACHGMT_000779"
     CDS             6431..7435
                     /locus_tag="ACHGMT_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6834548.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            7436..8380
                     /locus_tag="ACHGMT_000780"
     CDS             7436..8380
                     /locus_tag="ACHGMT_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6834549.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            8380..9342
                     /gene="opp4B"
                     /locus_tag="ACHGMT_000781"
     CDS             8380..9342
                     /gene="opp4B"
                     /locus_tag="ACHGMT_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFN6834550.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            9369..10283
                     /locus_tag="ACHGMT_000782"
     CDS             9369..10283
                     /locus_tag="ACHGMT_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6834551.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            10309..12090
                     /locus_tag="ACHGMT_000783"
     CDS             10309..12090
                     /locus_tag="ACHGMT_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN6834552.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            12438..13124
                     /gene="rnc"
                     /locus_tag="ACHGMT_000784"
     CDS             12438..13124
                     /gene="rnc"
                     /locus_tag="ACHGMT_000784"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFN6834553.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            13144..16725
                     /gene="smc"
                     /locus_tag="ACHGMT_000785"
     CDS             13144..16725
                     /gene="smc"
                     /locus_tag="ACHGMT_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFN6834554.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            16734..17543
                     /locus_tag="ACHGMT_000786"
     CDS             16734..17543
                     /locus_tag="ACHGMT_000786"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN6834555.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            17554..18552
                     /gene="ftsY"
                     /locus_tag="ACHGMT_000787"
     CDS             17554..18552
                     /gene="ftsY"
                     /locus_tag="ACHGMT_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFN6834556.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            18739..21006
                     /gene="gshAB"
                     /locus_tag="ACHGMT_000788"
     CDS             18739..21006
                     /gene="gshAB"
                     /locus_tag="ACHGMT_000788"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFN6834557.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(21095..21547)
                     /locus_tag="ACHGMT_000789"
     CDS             complement(21095..21547)
                     /locus_tag="ACHGMT_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFN6834558.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            21697..22647
                     /locus_tag="ACHGMT_000790"
     CDS             21697..22647
                     /locus_tag="ACHGMT_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFN6834559.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            22640..23599
                     /locus_tag="ACHGMT_000791"
     CDS             22640..23599
                     /locus_tag="ACHGMT_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFN6834560.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            23596..24351
                     /locus_tag="ACHGMT_000792"
     CDS             23596..24351
                     /locus_tag="ACHGMT_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6834561.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            24374..25327
                     /locus_tag="ACHGMT_000793"
     CDS             24374..25327
                     /locus_tag="ACHGMT_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN6834562.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(25971..26267)
                     /locus_tag="ACHGMT_000794"
     CDS             complement(25971..26267)
                     /locus_tag="ACHGMT_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFN6834563.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            26622..26978
                     /locus_tag="ACHGMT_000795"
     CDS             26622..26978
                     /locus_tag="ACHGMT_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFN6834564.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            26988..27887
                     /locus_tag="ACHGMT_000796"
     CDS             26988..27887
                     /locus_tag="ACHGMT_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFN6834565.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            28407..29135
                     /locus_tag="ACHGMT_000797"
     CDS             28407..29135
                     /locus_tag="ACHGMT_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFN6834566.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            29280..31307
                     /locus_tag="ACHGMT_000798"
     CDS             29280..31307
                     /locus_tag="ACHGMT_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFN6834567.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            31415..32512
                     /locus_tag="ACHGMT_000799"
     CDS             31415..32512
                     /locus_tag="ACHGMT_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFN6834568.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            32819..>33266
                     /locus_tag="ACHGMT_000800"
     CDS             32819..>33266
                     /locus_tag="ACHGMT_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6834569.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT        11139 a         5762 c         7125 g         9240 t
ORIGIN      
        1 aaaaaaccgt tcactatctg aacggtccaa gtatcattca attaaacgcg ttcaactttt
       61 cctgatttca aagcacgagt tgacacccaa acttttttag gttttccgtc gaccataacg
      121 cgaacttttt gtaagttagg tttaacagta cgttttgttg agttcatagc gtgtgaacgg
      181 ttgtttccac tttttgtctt gcgacctgta aagtaacaaa cttttgccat ggtaatttcc
      241 tcctttgcct tgatttggag caatagaata attcagctca taccagacta acatatcata
      301 tagaatggaa atttgcaagt acattttcat agatacaatt gacaattgag aagaataatt
      361 gtaaattaaa caaggttata cctgaaattt tgcaaggttt ttgagtactt tgggtaactg
      421 ccttttattt caagttgcgc tatgataaaa tatgatagaa gaaaaatggg agttttttac
      481 ccgttaagga ggctcattca tggctgtaaa aattcaaacg tcagctggaa ccattgaaat
      541 taccaatgat gtaattgcaa cagttgttgg tggcgccgcg acagatgtgt tcggtgttgt
      601 aggaatggcc agcaaaaatc aaattaaaga taatattact gagattttgc gtaaagaaaa
      661 ttactcaaga ggagtagttg tccgccaaga agataatggt attgctgtag atgtttatac
      721 gattatcagc tatggtacga aaatctctga agtttcccgc aacgtccaag aaaaagtgaa
      781 atacaatcta gaggcattat taggcgtaac tgccaattca gttaatgtct ttgtacaagg
      841 tgttcgtgta ctgccagact aggcaggaga ctggctggta actcagcagt cgatctgaag
      901 gaggattatc taggtgagtg taacagaaat aagtgcaagc cagttccagg aaatggtcca
      961 ggcaggtgcg aatcgtctgc aagtaaatgc agaatacgtc aattcattaa atgtattccc
     1021 tgtgccagat ggcgatacgg gaacaaatat gaatctatct atgactagtg gagctaaggc
     1081 agtagtagat tcaacctcag aaaaagtagg agaattagca gcaatgcttt caaaaggatt
     1141 gctgatgggt gcgcgcggaa attccggcgt tattttgtct caattgtttc gtggattttc
     1201 taagcaaatt ccagatgttg tctcattaaa tgcttccgat cttgctgcag ccttcactca
     1261 tggagttgaa actgcgtata aagcagtaat gaaaccagta gaaggaacga ttttgacagt
     1321 agctagagaa gcagcgaaag ctggagaaaa gaaagcaaaa agtactgatg atgtcgttga
     1381 agtaatgaca gctgttgtaa aaggggcaaa gcgtgcttta gctaaaactc cagacttgct
     1441 tccagtccta aaagaagtcg gtgtcgtaga cagtggaggg caaggactgc tgtttgttta
     1501 tgagggcttt ttaagtgcat tgaatggaga attccaagcg gatgaaacat atgaaccttc
     1561 tccagcagaa atggatgata tggtcaatgc agaacatcat cgcagtgtcc aaggtcaaat
     1621 ggcgacagaa gatatccatt ttggttactg tacagaaatc atggtaaaaa tcggagaagg
     1681 acctacagtc gatagtacat tcgattacga agaattccgt aactatttag atggtatagg
     1741 agattcttta cttgtggtca atgatgacga gatcatcaaa gtccacgtgc atacagaaaa
     1801 cccaggagaa gtcttgaatt atggacaaaa attcggttca ttagtcaaaa tcaaagtcga
     1861 caacatgcgc ttgcagcatg agacgatttt ggaacacgat gaagaagtga acgagtttgc
     1921 gaaaaaagaa acaccacgta tcccgtatgg tatcgtagcg atcgctgctg gtaaaggcgt
     1981 ccaagagctg tttgaaagtt taggcgctaa ttatgttatc agtggtgggc aaacaatgaa
     2041 tccaagtacg gaagacattg ttaaagcgat tcaagaagta aacgcagata aagtgattgt
     2101 cttgccaaac aataagaaca tcttcatggc agctgatcaa gcagcagaag tagcagatat
     2161 cccagtagca gttgttcctt caagaacgat ttcccaagga ttgacagcta tgctttcatt
     2221 caatgatcaa gcttctttag aagaaaacaa agaaacgatg acagaaatgt tgtctagcgt
     2281 tgtgagcggt caagtcactc acgcgatcag agatacctca attgacggtg tagagatcaa
     2341 agaaggagac tttttgggca tgatcgatgg aaaaatcctg atctctaacc cagatatcac
     2401 agcaacagcg ttaagtacac tagaacaaat gatcaatgag gacacagaga tcgtaacgat
     2461 cttggcaggt gatgaaggaa cagttgaaca agcaaatgaa tttgctgaga aactaacaga
     2521 aaaacatcct gatttggaaa tcgagatcca ccaaggagat cagcctgtct atccttatct
     2581 attttctgct gaataatttc tgtgtcagaa tatctttcta acacagtatt cattcataaa
     2641 aggccttcgg ttagtgatca ggtatgatcg ctgaccaagt cctttttttg ttaagggagg
     2701 gaggaaaaat ttatgcgttc tttagatgat ccaatcacta tgctggcagg agtcggagaa
     2761 aaaagagcag ctagtcttgc cagcttgggc atccaaacga ttgaagactt actgacttat
     2821 tatcctttcc gctacgaaga tattcaggaa agaaatctaa acgagatcca agatcaagaa
     2881 aaggttactt tgaaaggaat cgtagtctcg cctcctgtga tgaatcgatt tggttataaa
     2941 aaaagccgat tgcaatttcg tatgatgcaa gaacatgatg tgttcaacgt ttcttttttt
     3001 aatcaacctt atctaaaaga aaaagtcatt ttatcggagg aaatcgccgt atacggtaaa
     3061 tgggatgcaa agcgtaaagc attgaacggg atgaagattt taggttctca aacaatggat
     3121 gacttttcgc cgatatatca cgtcaacaaa tcaattcgtc aaacgacatt agttgatcta
     3181 atcaaaaaag ggtttcaaga atacggcaca ttgattgaag aaaatctgcc aaacaacctt
     3241 gtggagaaat atcgactgct tgaccgagct tctgctgtaa gaagtatgca ttttccaaaa
     3301 gaccacgaag aaaaccatca agccaaacga cgtgtcgttt ttgaagagtt ctttttattt
     3361 caaatgcgtc ttcaaggatt gaaaaaagca gaaaaagcag aaacgaatgg tttggagatc
     3421 ctttatgatg tccaaaaatt gaaagaattt actcaaaagc taccttttga actgacaaat
     3481 gcacaaaaac gtgtaacgaa tgaaatctgt cgggatcttc gcagtccgca gcatatgcag
     3541 cgacttttgc agggagatgt aggtagcgga aaaacagtgg ttgccgcgat tgcgttatac
     3601 gctaccgtta cagcaggttt ccaaggtgcg ttgatggtgc ctacagaaat cttggcacaa
     3661 caacatatgg aaagcttgca acagctgttt gatcccaatg aagtaaccat cgcattgctt
     3721 acggggtcaa cgaaagcaaa agaacgtcgt gagctccttg aaaaactaga gcaaggggaa
     3781 attaacatcg tgatcggcac acatgcattg atccaagatg gagtcgaatt tcagaattta
     3841 ggtctagtta ttacggatga gcagcaccgt tttggcgtga accagcggaa agtacttcgt
     3901 gaaaaaggct ggcgaccaga tgtcttattt atgacagcga ctccgattcc tagaacatta
     3961 gctattacag catatggtga gatggatgta tctatcattg atgaacttcc agctggacgt
     4021 atcccgatcg aaacacgctg ggtaagaaca cctcaattgg acagtgtact ggattggacg
     4081 aaaaaagaac tggcaagagg acatcaaatg tatgtcatct gtccgctgat tgaaggatca
     4141 gaagcattag atgtaaaaaa tgcagtggaa atctatgaaa aacttcgtga actatttgca
     4201 ccaaaatatg aagttggctt gttacatggt aaaatgaaga atcaggaaaa agaagtaatc
     4261 atggaagcct tcaaagacaa ccagatgcag atattagttt cgactactgt aattgaagta
     4321 ggggtaaacg tgccgaacgc gaccgtcatg ctgatcatgg atgcggatcg atttggattg
     4381 gcacagctgc atcaacttcg tggacgtgtt ggacgcggaa gtgatgcttc ctactgtatc
     4441 ttagttgcta atcctaaaaa tgaactaggt gtggaacgga tgaaaatcat gacagagacc
     4501 accaatggtt ttgtactaag cgaaaaagat ctggaattac gtggtcctgg tgaagtattt
     4561 ggcttccgtc aatctggtct gcctcaattt gcgattgctg acttggtgac ggatggaaat
     4621 gttttggagg tagccagaga agaggctgca gccctttggc agataaagga ttggcagatg
     4681 cttcctgagt atcagccttt agcgcaaagc ctccaaatag aaaatcagga taatcgttat
     4741 ttcgattagc agactgtgct atactttata ctgatgacgg aaaaaggagt ggaaacaatg
     4801 aaaattgccg tagatgcaat gggcggagat cacgcacctc aagcaattgt agaaggcgtg
     4861 gctttagcgc agcaagattt tccagaagta gaattcttac tttacggaaa agaagcagaa
     4921 atcaagaaat acttaacaaa tgaaaaaaac atcacgatta tccatacaga cgaaaaaatc
     4981 aatagtgatg atgagccagt aaaagcaatt cgacgaaaaa agactgcatc aatggtcctt
     5041 gctgcacaag cagtgaaaaa cggtgaagca gatgctgtat tttcagcagg gaatacaggc
     5101 gcgctactag cagcagggtt gttcatcgta ggacgtatca aaaatatcga acgccctggc
     5161 ttgatgtcta ccttgccagt agtagggaaa gaaggtgccg gtttcgatat gctggatctt
     5221 ggcgctaacg cagaaaacaa accagaacat ttgttgcaat acggtatttt aggttctttt
     5281 tatgcacgta aagtccgagg tatcggacgt cctcgtgtcg cactcttaaa caacggaaca
     5341 gaagcaacaa aaggtagtga agtgacgaaa aaagcctatg aactccttca gaatgaaaca
     5401 agcttgaatt ttattggaaa cgtagaagct cgtgagctac tcaatggtgt ggctgatgta
     5461 gtggtcacag atggatttac aggcaacgct gttttgaaat ctattgaagg aacagcaatg
     5521 aatatgatga atctattaaa atctgctatt ctaaatgaag ggatcaaagg aaaaatgggc
     5581 gctatgcttt taaaagacgg cttacgttct ttgaaagcag aaatggatta ttccaaacat
     5641 ggcggtgccg ttttgtttgg cttgaaagca ccagtcatta aaactcatgg agcaacaggc
     5701 cccgatgctg ttcgctatac gatccgccaa atccacacaa tgcttgagac agatgtagtt
     5761 ggacaattag tcgaacaatt tgaaaaaagc gaagaatagt atttggatat ctgaagcaat
     5821 ccgttccatt gaaaaaaagg tggacattcc tgaaaaatgc cagtaaaatt agccatggtg
     5881 taggtaattg acaagtgtat gattcttgga ggtgaaaaag ttgactcgcg aagaagtatt
     5941 taacaaagta gcgaaaatta tttcaaatca ttttgagata gataccgata aagtgacgga
     6001 tgaattgaac atcaaagatg atttaaaagc tgattcaatt agtattatgg aattcgtttt
     6061 agagcttgaa gatgaattcg gtacggaaat ttcagatgaa gacgctgaac aaattgaaac
     6121 agtcggtggc gcagtagatt atatcagcag ccatcttaaa taatttcaaa taaaagcaaa
     6181 gaaggataaa aagctgcata cagcttttta tccttctttt tttaatcttt ttgacacgta
     6241 aaagtcattt ttctgataat tctggcgata aacagaaatt atgttgcaaa gtaaagcgat
     6301 tttctatata attattaata attcattaca caaagataag aataaccttt gttataaaac
     6361 gctttattct gtgtaatgtc cttttttatt aagaaaaact tccatttagg aagcagagag
     6421 gggtattcga ttgtccgata accaattatt agaagttcag aacctacata caggattccg
     6481 actcaaagat gactattacg atgcagtaga tgacgtttca tttactttag acaaaaacga
     6541 gattttagca atcgttgggg aatctgggtg cgggaaaagt accttggcta ctacgattat
     6601 gggattactt gatcctaata acacaaaaat tacaggtgag attttatata acgatctgaa
     6661 tttaatcgac ctgaacgaaa cgctctataa taaaatccgc ggaaatgata tcggaatgat
     6721 tttccaagat ccgttgtctg cattaaatcc tctcatgaga atcgaagacc agatcaaaga
     6781 aggactttcc tatcacacaa agatgaatgc agaacagcgt caagctcgtg cactagaatt
     6841 gctagaacaa gtagggattc ctaatcctgc gagagttgga cgccaatatc cccatgaact
     6901 atcaggcggt atgagacaac gtgtcatcat cgcaatcgca atcgcctgca aacctccaat
     6961 cgtcattgca gatgaaccga cgacagccct agatgtaacc atccaagcac agattcttga
     7021 tttactgaaa gaccttcagg aagaaacaaa aacagggatc atcttgatca ctcatgactt
     7081 aggagtcgta gcagaaatgg ctgatcgagt ggcagtgatg tatggcggac agttcgttga
     7141 agtagcaggt gtaaaagaac tgtttgatca tcctaagcat ccttatacac gctctttact
     7201 gaactctata ccacaagaag gaaatcatga tgcagaactc catgtgatcg aaggtgtcgt
     7261 tccttctctg aaaaacatgc ctcgtaaagg ttgtcggttt gcaccacgta ttccatggat
     7321 accagcaagc gatcatgaag aagagcctat ccttcatgaa gtcgcaccga accacttggt
     7381 acgttgctcc tgctataagc atttccactt tagagacgaa aaaggagagg tgtagatgac
     7441 agaattaatc acaatcaaag acctgaaagt tcattaccct attcgcagtg gattcttcaa
     7501 tcgagtaacg gatcatgtat atgctgtaga tggtgtggat tttattatcg aaaaaggaaa
     7561 gacatatggt ttagtgggag agtctggctc tggaaaatct actacgggaa aagccgttgt
     7621 gggattagaa aaggtaacag ccggacaaat catgtatgaa ggaaaagatg taacgaagcg
     7681 aagcaaccgc aaaaaaatgg gttataacaa ggatgtgcag atgatcttcc aagattcaat
     7741 gtccagtttg aatcctaaaa agcgagtgtt ggatatcatt gctgaaccca tccgaaattt
     7801 tgaacggtta agcgatcaag aagaaaagaa aaaggtaaaa ggactgctag atatcgttgg
     7861 gatgccggaa gatgcgctat ataaatatcc tcatgaattt tcaggcggac aacgtcaacg
     7921 tctaggagta gcccgtgctg ttgctaccaa tccaaaactg atcgtagccg atgaacctgt
     7981 atctgctctg gatctatctg ttcaagcgca agtattgaat ttcatgaaac gaatccagca
     8041 agaatacggc ctcagctatt tattcatttc acatgattta ggtgtagtaa aacacatgtg
     8101 cgacaatatc gcaattatgt acaaaggtcg atttgtagaa atcggtacaa gagaagacat
     8161 ttataatgac ccgcgacata tctatacgaa acgtctgctt tcagctattc cgcaaatcga
     8221 tgtagaaaat agggaaagcc acaaagaaaa tcgacgaaga gtagaacaag agtatatcca
     8281 aaaccaaaaa gaatactatg atgcgactgg tcgagtctac gatttgcgta cgatcacaac
     8341 tacacacaaa gtggcgttga aagatggagg tgcaagctaa tgtggaaaac gattctgcga
     8401 cgtgttttat tgatgatccc gcaagttatt attttaagtg ttctgatctt cttattagcc
     8461 caagcgatgc cgggggatcc atttaccggt ttgatcaatc caaatcagga tccggcagtg
     8521 atcgaacaga tgcgacaagc agctggcttg aatgacccat ggtatgtcca gtacttccgt
     8581 tggatcggaa atgctttgca aggcgatttt ggtcaaagtt tcttatacaa acgctctgtt
     8641 gctgacttga tcggcgaacg gatcatcaac actttatatc tatcgatctt gacagttgtc
     8701 cttacttatt tgattgctgt gcctctgggg atgcttgctg gccggtatca aaattcaatc
     8761 ttggataaga ttgtcgtaat ctacaacttc tttagttttg ctgttccttt atttatcttc
     8821 ggcttgatca tgctatttgt ttttggttat cgtttaggct ggttcccaac aagtggatca
     8881 caaacactag gtttgcaagg tggatttatc actcaatgga tagaccgttt gaaatatatt
     8941 ctgttaccat caattaccca agcatttttg gcgactgccg tcacaatcca atatctgcga
     9001 agtgaagtaa tcgattcgaa atcgctcgat tttgtcagaa cagcccgttc caaaggggta
     9061 ccgacgaata aagtattcag tcgacatatt ttccgaaatg cagcgttgcc gatggcttct
     9121 caaatggggt atgaaatcac ttcgttgatt gctggttctg tagttatcga aaagattttt
     9181 ggttatccgg gagtcggaaa actatttatt gattccatcg gccaacggga ctataccgtg
     9241 atcacatcct tagtattgat tttaggaatc gcgacgctag tggggacatt actttcagat
     9301 atcatcatga gtatcgttga cccgcgtatt cggattcaat aaaaccaatc agaaaggaga
     9361 atggatcgat gagtgaaaaa aacaatacag cagttcaaga aagtattcct cctatgggga
     9421 tacgaatgat tgctcgagaa tttaaaaaag ataaaattgc tattttttca ttaggtttgc
     9481 tagtggtttt attattagtg atctttatcg gtgcgatgct gaccgatcaa gacaaagtga
     9541 tgtatgttag tatcttcgac aaatacgctg caccaggaac agtttcgaca caaggacaaa
     9601 agttcattct gggagcagat gaaggcggac gtgatgtatt aggacaattg atcataggtg
     9661 ctagaaactc tgttacgatc ggatttgcga tcacgatcct tacttcgatc atcggggttg
     9721 gattagggat catctctggc tactacggag ggatcattga caacatcttg atgcggatcg
     9781 ttgattttat catgattcta ccgatcatgc tgatcattat cgtcttcgta tcgattattt
     9841 ccagatacaa tgtctggtcc tttatattca tcatgagtgc attttattgg gtagctaaag
     9901 cccgattatt ccgaagcaag acattatctg aagcaagaag agattatgtc agtgcctcaa
     9961 aaacaatggg aaccaatgat tttaaaatca tgttccgcga aatcatgccg aatctaagtt
    10021 cattaatcat tactaatttg acgatgaact ttgcggcaaa catcggtatc gagacaactt
    10081 tgactttctt aggtttcggc ttgccggcaa atgtacctag tttaggtaca ttgatcggtt
    10141 atgcaagtaa cggagaagtc ttagtgaatc ggcaatggat ctggttaccc gcatcaatcc
    10201 ttattttagt tttgatgttg agtatcaatt atgttggaca agcattcaag cgttctgcag
    10261 atgcgcgcca acggttaggc tagtatagaa gcgaaggggg aaataggaat gaagaaaacg
    10321 atttttggat ttgcagcgct gatgtctgct gtcgttcttg cagcatgcgg gggcggaggt
    10381 acagatacct ctggaaacaa ctctggaggc aacggcaata acaaagagga attaactttt
    10441 ccaattgcaa caaaaaataa tgatgatgca atcgaagacg gcgaattaga agctgcggtc
    10501 gcaacggaca gtcagtttaa aggattgttc cagtgggagt tttaccaaga ttcatatgat
    10561 gcagctttta tggctccttc tcatgaaccg ctatttgcaa acgatgacga tttcagaatc
    10621 acagacgacg gagctgcaac gcttgatcta gatgaagata cgaaaaaagc gacaatcaaa
    10681 gtaaaagaca acgtcaaatg gtcagatggt gaagatgtaa cggcggacga tgtgattttc
    10741 ccttacgaaa tcattgggaa taaagattac actggtatcc gttatgacga cactttccgt
    10801 aacatcgtag gtatggaaga atatcatgat ggaagtgccg acacaatctc cggaatcaaa
    10861 aaaatagacg acaaaacagt tgaaatcgaa tataaagaag taaacccaag catgcttcaa
    10921 tcgggtggtg gtatctggtc atatgcagca ccaaaacata cgttagaagg tgtagcaatc
    10981 aaagatatgg aatcaagcga tcaagtacgt aaaaatccag tgacttttgg cccttattac
    11041 atgagtaata tcgttgcagg agaatcagta gaatacttgc caaacgagca ctactggaat
    11101 ggtacgccta aattgaaaaa gatcacgatg aaatctgtac cgatggcaag tgcaactgaa
    11161 gcattgaact ctaaattata tgatatgatt ttccaaatgc caacagatac ttacgatacc
    11221 tataaagaaa ttgctggcta tacaaactta ggccgtgaac aaacttctta tacttatcta
    11281 ggattcaagt taggtaaatg gaatgcagat acaaagagtg tggaatacaa tccagattca
    11341 aaaatggctg ataaatcatt acgtcaagca atgggctatg cattagataa taatgcagta
    11401 ggtgaacgtt tctatgcagg tttgagaagc aatgcgactt cattgattcc tccagtattc
    11461 cagtcatacc atgatgatga gatgaaagga ttcacacaag atctagacaa agcgaaaaaa
    11521 ctattagatg atgctggcta caaagatgtg gatgacgatg gtttaagaga agacaaagat
    11581 ggaaataaac taacaatcaa ctttgcttcg atgtccggtg gggaaacagc gcaaccattg
    11641 gcagattact atatccaaca atggaaagaa attggtttgg atgtccaata cacaacaggt
    11701 cgtctgatcg aattcaattc tttctatgat aaattagaaa acgatgatcc tgaaattgat
    11761 gtttaccaag ctgcttggaa tacaggaact gatccaaatc caagtggact atggggacca
    11821 aatgctgcgt tcaactacac tcgttatgaa tcagaagaaa atacaaaatt gatcaacgat
    11881 atcagttcaa gcaaagcatt tgatgaagag taccaaaaag aagcatatca aaaatggcaa
    11941 caatatgcat ttgaagaagc atttgcgatt ccaacgttgt tccgtaatga agtattgcca
    12001 gtcaacgacc gtgtcaaaga ctgggattgg tcttatgatg taccaaaccc atgggcaaat
    12061 gtttctgtta cagcagattc acgttcataa tcactaaaaa tgaccgaaaa agggcgatgt
    12121 cctttttcgg ttttttgata tttttctgtt tatttctcta atattcaggt caaggcgctg
    12181 atttcatgac ttctccaata aacggtgttc aaagtccagt ttctcggtat taagtcaaat
    12241 ttatgcaaaa atatggaaag ccattttcac aaaatttttt cttcatactc gaaactaaac
    12301 gaatttttca caacttctcg tcaatcacta ggattattaa agctttactt accttttgac
    12361 cgaaccattt caaatttgac aaaaaaccag tataataagg aagtatcgaa aaaatatcca
    12421 taaaaggaat gtggaaaatg gacaaccagc tgacaaaaga actaaaagaa aactataaca
    12481 tcgtttttca cgatctaaat ttgcttgaac aagcttttac ccattcatcc tatgtgaatg
    12541 agcatcgcaa tctgcaatta tcggataatg agcgacttga atttttagga gatgccgttt
    12601 tagaactgat ggtttctcag tatttattcc gtttatatcc tcatttgcca gaaggaaaat
    12661 tgacaaaaac acgagctgcg atcgttcgcg aagacagctt gtcgaaattt gccaaagaat
    12721 gtcactttga tcaatatgtt ttgttgggca agggagaaga gaattcagga ggacgtacgc
    12781 gaccagcatt gctttgcgac ctattcgaag cttttttagg cgcgttatat cttgaccagg
    12841 gattcgacac gacccatgca ttcatcgaaa aagtgatttt tcctaaagtc aaagcaggtg
    12901 ctttttcaca tgagatggat cataaaacaa aactacaaga agtcttgcaa aaatcaggtg
    12961 atgtatcaat cgaataccga ttgatcaatg aagaaggtcc agctcatgaa cgagtatttt
    13021 ggatcgaagt ttatgtagat gatcaattga ttggcactgg acaagggaaa tcgaaaaaat
    13081 tagcagaaca agctgcggcg gaaaatgcat tggcagcact gtaaaagtaa gtgagggaaa
    13141 gctttgtatt tgaaacgaat tgaaatagcc ggttttaaat catttgcaga caagacagtc
    13201 attgattttg aaaatagcgt cacagcagtg gttggaccaa atggcagcgg aaaaagcaat
    13261 attacagagg ctatccgctg ggtattagga gaacaatctg ctaaaagtct gcgaggcgga
    13321 aaaatgccgg atatcatctt tgccggttcc gatacccgta agcagctgaa tattgcagaa
    13381 gtcacagtga ttttagacaa tagtgatcat tatctgccat tggagtacaa tgagatcagt
    13441 gtaacccgcc gttatcgacg gactggggaa agcgagtttt tcatcaataa acaaagctgc
    13501 agattaaaag atatccaaga attgttctta gattctggat taggaaaaga atcgttctca
    13561 atcatttctc aaggaaaagt agaagcgatt ttcagtagta aaccggaaga ccgtcgtggg
    13621 atctttgaag aagcagctgg tgtcttgaaa tacaagcagc ggaaaaagaa agcagaacag
    13681 aagctttttg aaacagaaga caacctcagc cgcgtccaag acatcatcca cgaattggaa
    13741 gaacagttaa ctcctttggc cgcccagagt gaggcagcaa aggagttttt acggctaaaa
    13801 gaaacactga ctcaaaccga tgtctctttg atggttgctg aaatcaagac agcaaaaaaa
    13861 gattgggaca acaaacaagc acagcttgcg aaattcaatc tagaattagg caaactatct
    13921 gagtcgatcc aagaacaaga atccattttg gcgaagcagc gtaaagaaaa tgcgcaagcc
    13981 gatcgtctga tcgaaaaaaa tcagcaagta ttattagatc tttcggaaaa actgaagcag
    14041 acagaaggac aaaaagatgt tttgcaggag cggacaaagc atacacaaaa aagctcgcaa
    14101 gaatatcaaa cttcattagc agaagcacaa aaaaaggtta aacattttga aaagcttcaa
    14161 gaaagcttga tgaaagcagc agcagaaaaa gaaacagaga tccaaaaagc tgaagcaaat
    14221 ctcataaaaa cacagcaaga attagaaaaa taccaaaaat caacgaaaga attattggca
    14281 gaattaagag atcaatatgt tgatttgatg caagaacaag cagctgtagg aaacgaactg
    14341 aagtatctag aacgtcaata tatacaagaa acagcaaaaa gcaagcagac gttagccaag
    14401 caatcggaag tagaagcatc agtagaccga ttgatcttac aaaaagaaga attgacacaa
    14461 aagcaggcgc agctaaaatc atctttaact gaaacaaaag agaagttaga aatgatccaa
    14521 caaaacggga aaaagttcca agaagcatta gcaaaagaac agcctaaaat gtatcaatta
    14581 atgaatcaag tccagcagct acgtgctcgt aagaaaagcc ttcaagaaat ccaagaaaac
    14641 tattttggtt tttaccaagg ggttcgattg gtcttgcagc ataaacagca gctttcgggt
    14701 attgtcggag cagtggctga actgatcgat gttcctgcag atttcacgtt agctatcgaa
    14761 gcagctttag gtggggcagc ccagcatgtc atcgttgaaa atgaaaaaga tgctcggcaa
    14821 gccatcacgt atttgaaaca gcaaagagga ggaagagcaa cattccttcc actgacgaca
    14881 atcaaaccaa gacaattacc ggctcatata ttgacacaag cagcagctgt agaagggttt
    14941 atagggattg ccagtgaaca agtcagttat cccgatcaaa tccaaactgt ggttcaaaac
    15001 ctgctaggaa cgatcctttt agccaaagac ttaactagtg ccaatgcaat cgcgcaaacg
    15061 atccgctatc aatatcgagt ggtttcctta gaaggtgatg tgatgaatgc cggaggatcc
    15121 atgacaggtg gagcgaataa acgaggaaac caaggcagtt tgtttgtaca aaatcaagaa
    15181 ttgaaacagt tgactagtga atttgaagaa gcagataaac aactccaagc acaagaaaaa
    15241 aaagttcagg agctgcaaca agaaacagct cgtttagccg aagaacaaga agttttgcgt
    15301 acacgaggcg aacaattacg ttttgaagaa caagaagcga caaatcagct acaaaacatc
    15361 ataaatgaac ttgaacgttt tgaaaaagaa aagcaaatct caacttttga gacacgtgaa
    15421 ctgcagcagt ttatcgaaga ttacgaaaag caaacgaacg aattgaaaga caaacaaaca
    15481 gatcttgaat cgcaacgaca aaaaatcgac gaagaaatca aatcgttaag tcaagaaagc
    15541 gaccagatgg aagccagaag agcacaagta caaagtcaaa aagcacaaga acaagcagat
    15601 cttgctgtct tgaaagagca gtttaatcat ctgcagatcc agctccgcgg tgcccgagtc
    15661 caaaaagcag aggcaaccga acgtcaagaa gcgatagaaa agcagttggc gacattgaca
    15721 gcagactttt ctgatcatga agtcacagaa gaaagtttgg aaaaacaaat taacgaatta
    15781 tctgctcagc gagaaacatt aaaagccgag ttggcaaaag ccaaagagca gcgagataga
    15841 aggcaaaagg agatcgataa gcttgaagca gtcttggcag agcgtaatcg tgagcaaaaa
    15901 gcacgactgt cggaacaatc caaattagaa gtccaaaaag accgagctga aatgatttta
    15961 gataatcatt tgacctatct gcagtcagaa tatcagctga catttgagaa agcttcccaa
    16021 gactatcaag aaacaaccga tatcgaagac agtagaacaa aggtttcatc cttaaaagaa
    16081 cagattgaaa aactgggacc agtaaactta aattcaatcg aacaatacga acaagtcagt
    16141 gaaagacata cattcttagc aacacagcga gatgatctgt tagcagcgaa aaaccaattg
    16201 tttgaaacga tggatgagat ggatgatgaa gtgcggacaa gattcaaaga agtatttgaa
    16261 gctatccgtc aagaattcaa aattgtcttt ccaaatatgt ttggcggcgg taaagctgaa
    16321 ttagtactga cggaaccaac tgatttgctg aaaacaggta ttgagattga agtacaacct
    16381 cctggcaaaa aactgcaaag tctaagtttg ctttctggag gagaacgagc acttacagcc
    16441 atcgcgttgc tcttttccat tatccgtgtc tgtcctgttc cgttttgtat cttagatgag
    16501 gtagaagcag cgttagatga agccaacgtg aaacgttttg gtcgttactt gagcgatttc
    16561 caagatgaca cacaatttat cgtagtcaca caccgcaaag gaacgatggt cgcagcagat
    16621 gtactgtacg gagtgacgat gcaggaatcc ggtgtatcaa aaatcgtttc tgtacggatg
    16681 gaagatatca atgaagaggc gaaaatcaac gaaacgaaag tgtaggaacg aaaatgatca
    16741 aattagtagc aatcgatttg gacggtacat tattaaatga acaaaagctg atttcagatg
    16801 agaataaaca agcattagca caagcaaaac agcaaggggt caagatcgtt ctatgtacag
    16861 gacgcccctt agctgcgatg gcgcattatc tgcaagaatt aggactggta gatgaaggtg
    16921 actacagcat cactttcaac ggcggtttgg tacaaaaaaa tgatactggt gaaatcatgg
    16981 aaaagaaagt gatggaagta tcagatatcc aacgcttata caaactggct cagcaattgg
    17041 atctgccact agatgttttg tctgacaatg tggttttaca gctgcctagt gccccaagga
    17101 agcaatcctt gtacaatgtg ttgaataagc tgctgcaatt ccaaccagcg gatataagcg
    17161 atattacaga tgaatttgtg cttaataagg cagtaatcgc ttatgaccaa gaagaattag
    17221 atcctaaaat caaagaaata ccagcagaat tccatgaaat gtatgagatc atcaaaacaa
    17281 gaagtatgct tttggaattt atgccaaaag gtgttacaaa agcttatggt atttctttat
    17341 tggcaaaaga tcttggtttg aaacaagaag aaatcatggc gatcggtgat gaggaaaatg
    17401 atttaccgat gatccaatat gctggactag gcgtagcgat ggagaatgct gtgcctttcg
    17461 tcaaagaagc agcagatttc gtgacagcca gcaatgaaga aaatggcgta gccaaagtga
    17521 tccaaaaatt tgtattagag tagggggagt caaatgggat tttttgataa aatcaagcgt
    17581 gcttttactg gcgaagagaa aacagaagaa gttgaagtac aggagaagta cgacaagggt
    17641 ttagaaaaga cgcgtaaaac atttggtgaa cggatgaatg agttgtttgc caattttcga
    17701 acagtcgatg aagacttttt tgaagaatta gaagaaacac ttatcggtgc agatgtaggt
    17761 tttgaaacag cgatacgaat caccgaagca ttacgtcaag aagtgaaact cagaaatgcg
    17821 aaaaaatcgg cagaagtcca aaatgcgatc atcgagaaac ttgtggatct ctatgaagcg
    17881 gaaggaatca atgaagtaaa tgaactaaat atccaagaag acggactaac agttattttg
    17941 tttgtcggtg ttaatggtgt agggaaaaca acaagtattg gtaaacttgc ccatcagttc
    18001 aaaaacgaag gtaaaaaagt attgatggca gcagcagata ccttccgcgc tggagcaatc
    18061 gaccagttag ttgtctgggg cgaacgcgct ggtgttgaag ttgttcgtgg caatgcagga
    18121 ggcgatccag cagctgtcgt attcgatgct ttggaaagag caaagacaga gcaggcagat
    18181 gttttactag ttgatactgc aggccgactg caaaataaag tgaacttgat gaacgaatta
    18241 gaaaaaataa aacgagtaat ccagcgggaa ttaccagaag caccccaaga aacacttctt
    18301 gtattagatg caaccactgg tcaaaacgcg atggttcaag caaaacaatt caaagaaaca
    18361 acagatgtga ccggtcttgt tttgacaaaa ttagatggta cagcaaaggg cggtatcgta
    18421 cttgctatcc gtaatgaatt gcatttacca gtgaaattgg tcggtcttgg tgaaggaatc
    18481 gatgacttgg agccgtttga tccaaatgat tttgtcgttg gattgttcaa aggattacta
    18541 aaagaagaat aacccatgtc taaaaagcag gagctgtgac aaaagttgca gctccttgtt
    18601 tgaatgattg tttttaacat agctgtgttt ttgatgcctt catttttata cttatttgta
    18661 agcggttctc aaacaatttc gttctttttc ttagactttt tgattataat tagattcata
    18721 tgaattggag gtcacttaat gatgaatttt aagcaattat tattgcatgt caatgcgcgc
    18781 ccttttatcg accaagctcg atttggtata gaacgggaag gacagcgggt tgatcttgca
    18841 ggaaatctag caaaaaccga tcatccagca atctttggcg atcgatccta tcacccctat
    18901 atccaaacag attttagcga aacacaaaca gagatgatca cccctgttac cgattctatt
    18961 cccgaattat ttcagtatct cgctgctgtt tatgatgtga ctgctcgttc tataccgaaa
    19021 gaagagatga tctggccatt aagtatgcca cctgccttac cggaaaaaga cgaagagatc
    19081 attattgcaa aattaaaaaa tttcgaagat gtcttgtatc gacgttattt agcaaaaaaa
    19141 tacgggaagc gaaaacagat ggtaagcggt attcatttca attttgaatt tggcgatgag
    19201 ttgctaagaa cattgttcag ccatcaggaa gaatttcaag atttttccga attcaaaaca
    19261 gaactttatt tgaaaacagc gagaaacttt atgcgctatc gctggatgat cacttattta
    19321 ttcggtgctt caccaatgag tgagaagaac tactttttag atgaatcaca tccgcaagaa
    19381 cctgttcgca gtattcgcaa cagtgcgctg ggctatacga atcatccaaa tgtgaaagtt
    19441 tcctatgctt ctatgaaaca gtatttagca gatatcgagc gaatgatcga agaagggaaa
    19501 ctttcggagg agaaagaatt ttatacaccg cttcgtttcc gaggcggaaa gaaagtcgca
    19561 gatttagcaa caacaggtgt tcgttatatt gaattgcgga atatcgattt gaatccttat
    19621 gcaagattgg gtatcaatcc agagcaagtt cggttcttac aattattcct gatgtacatg
    19681 ttatggacag aagaaaaaga agactgtgac caatgggtag cagaaggtac gactcgaaat
    19741 aacaaagtag cactagaaca accatcagat caaacagaat ttcatcaaga aggtagagaa
    19801 attcttgaag gaatgaagca aatgctggtt gaattggatt ggttggattc tctttattta
    19861 gtagaggaag cattgactca aatggatcat cctgaacaaa cgttagcagc gaaactctat
    19921 caagaagcac agctatcgag tcagcaagaa gttgctgtgg cattgggaca tcaatattat
    19981 aaagaaagcc atgaacgtcc ctatcagttg gccggttttc gcgaaatgga actttctact
    20041 cagatattta tgttcgatgc catccaaaaa ggtgtccaag tcaaagtatt agatgagtcg
    20101 gatcaatttt tgcgtctgca attccaagac catgtagaat atgtgaaaaa tgcaaacatg
    20161 acaagtaagg acagctatat cgtcccactt atcatggaaa acaaaacagt aacgaaaaaa
    20221 gtcttaaaag aagctgggtt ccgggtacca ggaggcgcag aattttcatc catggaagaa
    20281 gcggtaaaag cttatccgag atttgccgag caggcatttg tgatcaaacc taaatcaacc
    20341 aattacgggt taggtatcac gattttcaag gaaggagcta gtctggaaga ttatcaagca
    20401 ggtttagcca ttgcttttcg tgaagacagt tcggttttag tagaagaatt tatgccggga
    20461 acggaatatc gtttctttgt gatcgacgga gaagtccaag ccatcatgtt gcgagtccct
    20521 gctaatgtta ttggtgacag tatccgaaca gtgaaagaac ttgttgaaga aaaaaatagt
    20581 gaccctttgc gtggaacgaa tcatcgtgca cctttagaat tgatccaatt aggtgaatta
    20641 gaacaattga tgttgaaaga acaagggttg acgatcgaat cagttcccca agccaatcaa
    20701 attgtttatc taagggagaa ctcaaatatc agtacaggtg gtgattcgat cgatatgact
    20761 gatgaatttt ctgaagctta caaaaaaatc gccgtttctg cggtagaagc actaggagct
    20821 aagattagtg gtatcgattt gattatacca gataaagaaa tcgatcctac aactgataaa
    20881 aaagcatatg ggatcatcga agcaaatttc aatccagcca tgcatatgca tgtttatcct
    20941 tttgcaggaa aaggcagaag attgacgatg aatgtattga aattgttata tcctgaagta
    21001 ttttaaaaga aaaagctttt gacagattgt cctatttgat gtcaatctga tcaaaagctt
    21061 tttttgcatg taaaaattta tgtgtttttt cgttttaaaa taaacgatcc cagaagcttt
    21121 ttttcttttt tggtttttct ttttcagctg gcgaagagtt ttcttctggg tatttttttt
    21181 cgatatcaat cacaggttca tttactgctt cttttgctgt taagagcaat ccgaattcct
    21241 caggatttgc agtgacgtaa tcgttgacta cagtgaaacg gctattgttt ttcgttgcta
    21301 gctggatata ttggttttgg acagaaaggg gtagagcccc attcaagagt atggaagcat
    21361 ctggatgttt tttgagttct tttaaaaagt tttctttgtt ttttggttgg atcatttcag
    21421 caacagtcat actaagatag cagcgttccc tgaatgtccc taaatattta tgctgttctt
    21481 cagggttcac taaaggtgta ccataaagtc ccttatccaa atggcttttt acatcatctt
    21541 gtgccataaa aaataccctc cttcttctct ttgtctttct tacgattata gcatattccc
    21601 aataatacct aaaacatatg aaaaagcggt tgcttttgtt caacgattca cttaaaataa
    21661 gaatgaaaat cattctcaat tagaaaggtg gattggatgc gctacggacg actagtagca
    21721 ttattagtta ttttaagtag tatttcattg ttcatcggag tatcgaatgt acaattgtca
    21781 gaactgctcc agttagatgg gcaaagctta caaatcttgc tagtgactcg tttgccacga
    21841 acgatttgtt tgatccttgt gggagcgacc agtagtattt gtgggttgat catgcaacat
    21901 ttgacccaaa ataaatttgt ttcaccaacg actgcgggca cgatggatag tgcgcgttta
    21961 ggtattttag tggctatgat tttcctacct ggtgcatcct tgattgtccg atccttgaca
    22021 gcattttgct ttgcttttgc aggaacattg ctttttttat cccttacgcg attgttcctc
    22081 cagaaaaatc aattgatgct tccgttgatt ggagtcatgt tagggaatat cattggttca
    22141 gttgctactt tttttgctta tcaattccag ttggtacaaa atatgacttc atggcttcaa
    22201 gggaattttg cgactgtgat acgaggaaat tatgagttat tgtatgctgt tgttccattg
    22261 ttgctcttgg tttggctatt tgcttaccga tttacgatca ttggtctggg cgaatcgttt
    22321 gcagtaaatc tgggaataga ttaccagaag atgcagtttt taggcattgg cttagtatca
    22381 ttggctagtg cagtcatgtt gatcatggta ggaagcattc cttttatcgg agtgatcatt
    22441 ccgaatcttg tctctagaat atatggagat caagtgcaca aaacaattgg cgtaactgct
    22501 ttagcaggta gtttgttctt agtattttgc gatcttgtcg ctcgcgtgtt gatttttcct
    22561 tatgaaatac cagtcagtgt gatcgttgga atcatcggtg gggctatctt cctttatctt
    22621 ctgataaggg gacgacgata tgcttaaaaa aaagccatct atttttattg gtatgcttgt
    22681 tcttgtttta tttgttacag gaatgtactt gacgatccat acatatggaa actggcattt
    22741 tgcatgtgca ttgaggagta agaaaatttt tgcatttatc cttgtctcgc tagctgtcag
    22801 cagtgccaca atcagctttc agacaattac gcataaccag tttttgactc cttcagtatt
    22861 gggacttgag tcgttatatg tccttgtcca aacggtcctt ttcttcttta tcggaggagt
    22921 gacgatgctt cagcagacag gtacagtttt tttcttattg aacgttgggc tgatggtcgg
    22981 gctgagtcta ttgttatttt tctttctttt gaaaaaaatg agagcgaatc tttttttatt
    23041 actgatgtca gggatgattt taggtacgct ttttggtagt gtcagtacct ttttacaagt
    23101 catcatggat ccgaatgaat atgatttgct tcaaggcaga ttatttgcaa gctttggcaa
    23161 tatccagagt cagtatcttt tgcctgcttc tttgatcatt atcggctgct ggtacctgtt
    23221 atttcgatat cgacatgtgc tagacgtttt ccatctcgga aatgaacaag cagtaaacct
    23281 cgggatagac acagatcgtt ttgaattttt gattttatgt tgtgtcagtc ttttgacagg
    23341 gacatcaacg gctcttgtgg gtccagttac ttttttaggg tttattgtcg caaatatcag
    23401 ttatcgtgtc ttttctactt atcgtcatgg tcagctattt atcggaagct ttctggtagc
    23461 tatgctgttt ctgacaggag gacagctcgc agtagagcag attttcggct ggaatacaac
    23521 agtgagtgtc gtcgttgaat ttgtcggagg aatttatttt gtcacaaaat tgattcgcga
    23581 aaggaagagt atcctatgat tgatttacaa aaggtcaata aactgtacca tggaaaatca
    23641 gtggttaaag atgtacagtt aacgattcct gaaaagaagt tgactgcttt tatcggtcca
    23701 aatggtgctg gaaagtcaac ggtgttgtcg atgatcagtc gtttgatacc aaaagataca
    23761 ggtgaaatct atttagacca taacgaagta aaggcttgga aatccaatga attagccaaa
    23821 aaattatcga tcctacgaca atcgaatgaa ataaatgtcc aaatcaccgt gaaagaatta
    23881 gtagaatttg gacgatttcc ttatacgaaa ggaaaattga cgacaaaaga cgaggaaatc
    23941 gttcaggaat cactggaaca ccttgggtta gaagaaatag cggatcaatc gattgacact
    24001 ctttcaggcg gtcaactcca acgtgcgtat attgcaatgg tgctagcgca agacacggat
    24061 tatattttac tggatgaacc tttgaacaat ctagatatga attttgcggt tcaaatcatg
    24121 cagattttaa aaaatttagt acaagaaatc gggaaaacaa ttgttattgt tcttcatgat
    24181 atcaactttg ctgcaagtta tgcagacgaa attgttgcaa tgaaagatgg acagttattt
    24241 acgcaaggaa cgacccaaga aatgataaaa aaagatgtat tggatgaatt atacgaaatg
    24301 gacatacgga tttgtgagat tgaaggccgc agattctgtc tttatttcta aaactaacat
    24361 taggagagaa gagatgaaaa aattagtagt aggtatcttg attctagcat catttggttt
    24421 agcagcatgt ggaaatagca acaacaccag tcaggcagac acaaaaaaaa gcagcacaca
    24481 aactgaaact acattgacga taacagatag taatggagac caaatcgagg tgccaaataa
    24541 tccagaaaaa gtcgtcgtat tcgacaatgg ttctttagat acgatggatg ctcttggtgt
    24601 tggagataag gtagttggag cagctacaag cagccttcct gagtatcttt catcctataa
    24661 aaaagtagaa tcagcaggtg gaataaaaga accagactta gaaaaaatta atcagcttca
    24721 gccagatcta atcatcattt ctggccgaca aagagatttt caaaaagatc taagtcaaat
    24781 tgctccgacc attttccttt ctttagatgc gaaaaatcca tgggaatctt tccagcaaaa
    24841 tgtcactgct ttaggggaga tctttggtaa gcaagaagaa gctaaaactc aattagagga
    24901 attatctagt gcaatcgatc agacgaaaaa gaaagcagaa gctactgaca aaaaagcact
    24961 agtcacactt gtgaatgaag ggcaattatc agcctacgga tctggttcta gattcggttt
    25021 catccatgat ttatttggct ttgaacaagc agatgatcaa atcgaagcat ccactcacgg
    25081 tcaaagtgtt tcttatgaat atgtgctgga aaaaaatccg gatatcttat ttgtagtaga
    25141 cagaacaaaa gctattggtg gagacgacag caaagatgat atttctgcca atgaactggt
    25201 agcacaaacg aatgctggta aaaatcagca gatcatctcc cttgaaccag atgtctggta
    25261 tttaagcggg ggcggtttag aatcaatgaa actgatgatc gaagatgtga atcaagcatt
    25321 taaatagcat aaataacaga ggattaaact ctcatactat gtaacaataa tgtttcttac
    25381 attcttaaat agcaagagcc tatcctcaaa gaagttatca aaaaccatcc gtaaaatcgt
    25441 attgatgatt ttacggatgg tttttattta tttgagaaat cttttttcaa cgtgaattca
    25501 ttatctacat catacgggtt gattctagaa tggggagtgg caaaaaatag tgtgatccat
    25561 tttttcagca tcaagatcgc ctcctttgat ttgaatcaaa ggtacaaagc gaaacttaga
    25621 taaaacttaa atttagatat ttttattttg taccgatctc ttctttaaac gatgttccat
    25681 ctgctgcttc tcggcaataa gtccactaga ctagaaaata tgagaagcta ttttctgtcc
    25741 agcgttctta ttactcaaaa caaatcgcag ttgtcacaat ctcttctaaa cgatgttcca
    25801 tctgctgctt ctcggcaata agcccactag actagaaaat atgagaagct attttctgtc
    25861 cagcgttctt attactcaaa gcaaatcgca gttgtcacaa tctcttctaa acaatgttca
    25921 atcagctgac cttcggtatt agctcaaagc actgatttca caacctctat ttaggcatgt
    25981 gtctcagcaa gcggttgata gttgatgatc tgcatttttc gataaaaatc ggcgggaagc
    26041 ttactccacc gttttagtaa ttgatagaaa taactctctt ctgctttgat tttatactcg
    26101 cttaatatca atccaaaact attcgttttt tttgaagcta attttttata aatatccaat
    26161 gctttggttt tatcttcatt gacaaagagt gtttttactt caatataaga accctcgata
    26221 ttttgtgttt cttcgataaa atagatgttt tttatatatt ccttcatagt gattcctcca
    26281 aagaagtctt ttatttgata agtttactct accgaagagt tgttaataat ccgtgaacaa
    26341 aaagtaattc tttttccaat agttgttaaa ggttttttat tttttatagt attttttgtt
    26401 atttaagaaa ggaataaggt taaacaaata acaggttgct ttttgggtct ttttccgttc
    26461 aagaaactga acgaagaaaa gggaaagttg tctcttttgt gataagagaa atcataataa
    26521 acgaatgact tactttccaa taaattgaga acagtcagaa ccagacaaat tagttgcatc
    26581 tgatgaataa agtgattatt ctatgacatg aggtgataca gatgaaatta gaagtgacaa
    26641 aagaagcaca agaagtattg aagaataaat tagaagcaga cgatcaactt ttattagata
    26701 tcgaaaacgg tgacggtcca tttgcagaaa gccaagtcag ttgccaattg gatacggcat
    26761 ttcgattgat cattgtcaaa aaagatacac aaacagatct aagtctttat tctgtagtgg
    26821 ctgatacacc agttggacca atcaaaatca aagacagtgc aatactttac atggatgatc
    26881 catccacact tgctttagaa ccaacttata atagtttaca attaaaagga ccaagcggct
    26941 tgagaaaagg caacttgcaa gttgttcgaa aagactagag ggataaaatg ggaaaattgg
    27001 cgatcatcag cgacctacat gtggacatca ataaattcgg cgaatcagag ctgatgcagc
    27061 tttggcaagt tttgcaaaag cacaagatca ctcggcttca tctagccgga gatacggcta
    27121 ataaagtaga ccgttgccta gctgttgtcg aatttttcgt caataaagga ttatctacga
    27181 catttcattt cggcaatcac gagttggcag atgtgcccaa tgaacagatg atggaccatt
    27241 ttccagaccc acatttttta aatgaacggt atattgcttt aaatgagcag accgttcttt
    27301 tagggatgaa tggctggtac gactatcagt tttccgaatt gaaagattac gaggcaatcg
    27361 tacgaatgaa gcgtttgtat tggtatgatc ggttcatcca gcgtgggaaa acagatctcg
    27421 aggtcaatgc agaaatgctt gcagctgcaa aaaatttgct agaccagctg gaagcaaaaa
    27481 aacttgaggt gattttatcc actcatttcg ttcctaaacg ggcatttatc atgtatcaat
    27541 ctgctcctta tgagcgttgg aacaaattaa atgcattttt aggatcagaa tcattcggag
    27601 aattacttga ccgttattca aatatcaaac aagtagtttt tggccatacg catcgtcgct
    27661 ttgaagataa atccatcggt cacacgattt acagctgcag accttttggt tattattatg
    27721 aatggcagct gacccgagat tttgtcttga aagaacagtt gctagacagc tatgatccta
    27781 tgaaattacg tgtcctcttg cgtgcccatc acccagcttt tcaggcctat caaacacaac
    27841 atttgcaaga ggaatttgaa caagcgatga caatcgttcc gtattgagac agcattagta
    27901 atgtgtaaaa gaaaatacct tgtaaaacat aaggataagt aagagcagta gatccattta
    27961 tcgtggatat gctgcttttt ttgcggaaaa taatcagcga atgtttattt gatcggatga
    28021 tttgtaactc gcagagtact agatgcaagc gtgtattctt tttttgaacg ataatcacaa
    28081 aaaaaaatat aatatacatt ttttatttaa ttataattaa tattgattga agtctaggca
    28141 gtcaagtcac attttgatag ataggagggg gacttacgaa ttattttaaa ggctatgaca
    28201 gctgtatttc tagattgtgt ttgcatcaag ttgcaataaa aactagaaat caaatcgggc
    28261 tgataatcat ttataactaa tcgatacata ggaccaaata taggtctatg ttcttttatt
    28321 tcataagtat cgtaaaacag aattttgcta tggattaatc caatcgactg atatgtttag
    28381 taaataaaga aagaggaatg tcaatgatga aaaaagaaat cgtattaaca gcagcaatga
    28441 tgttattcag catggtagca agtacgactt ttgtttctgc aactgaagta tacccaaagg
    28501 aatataacac agaaggaacg attacatttg aagcaggaga tgagggtgta actcctcctg
    28561 ttgatccaga gaatcctgat cccaataaac ctgtcgatcc atcagatcct ccaagccctg
    28621 gaacaggagg ggcattatcg attgattatg gatcaaaatt caaatttggg actcaaaaaa
    28681 tttctactgc ggataaaacg tattatgcag ctgctgatgt gatgaacgat gggtctagaa
    28741 aaccgacata tgtccaagtg acagatagga gaagtacctt atctgggtgg aaattaagtg
    28801 tctctcaacc agaacagttt aaaactgcca gtggggatga actagttgga gcacagctga
    28861 aatttacgaa agggcaggct gtttctttgg ttgatccaac atatacgccg caaacggtga
    28921 attctgaatt aacgttgaca ccgggaggaa acaatacgtt agcaattaat gctaaatcag
    28981 gaacaggtgt ggggacttgg gtctatcgat ttggtgcaaa tgaaaatgaa aatcaagatg
    29041 cggtacagct ttcggtaccc ggtaaatctg tcaagttagc gcaacaatat tccactaaat
    29101 tagtttggac attagaagat acacctaaca actagtttac tgaaaagaac tgttaaaaat
    29161 aaatacgtag tcatattgtc ttgcgaggta atcgagcaaa aattgtcaaa ggctaggcta
    29221 aatttgtttg gatacgaata gttcatctag gaatttaacg atcaggagga aaagatatat
    29281 tgaaaaagag gaggatcgtg ctcattttaa gcagtctctt agtgactaat atctttccga
    29341 tcatagggta ctctttggaa ttgaatcagt tggagcatgg aaatagaaga gacgaagaaa
    29401 gcaaactcac ttcgacattc gacagtttaa tagaatccaa ccagacacaa gaaagcagtg
    29461 aggatttaag tatttcgagt agtggagaga ccactgacaa cgtagaagct gagggttcgg
    29521 acaaacaagg actagaagat gcaccaattt ctgcggttga gctgcctgac gatcccaata
    29581 ttatttctat cgataaaatt tttcaaacac ctatcggtac tagcacgtcc atattggaaa
    29641 atgggaagtt actgcagttg aacccagcag aaaggtctca aagaggagct atttggtcaa
    29701 ataagaagat tagtttactc tcggatttta catttaaatc ttatttatac ttaggaaatc
    29761 aacgagatag agcgggagat ggcatgacct ttacattgac tggcgatcct agaatggcta
    29821 gtaatcccag tcaagttatt ggatcgccag gtatgggaat tggctcatat tctactcgtt
    29881 ctggccaacc ttacattgcg aatgcgctct cgattgagtt tgatacgtat aaaaatagtg
    29941 gttcttctga tcgaatggat cgagagataa ctgctgataa tagagggcat ggacatgtcg
    30001 cttttgtcac gcctaaacca aataataata attattctgg agagcatact ggcgtgacgg
    30061 tagctcctac atttctttct aatggcacat ggcgcttgtt aataattaaa tgggacgcgg
    30121 agcaaagaag tcttacttat gatttggaag gtgtcggtcg ctcttctcgg attatacctg
    30181 atctcattag gcagtttggt tctacagaag taaattgggg gtttacttct tcaacaggta
    30241 ctttttttca agaaaatgct gtcgctgtca cgcgacttcc atcgaatgtc acatcaacag
    30301 ctgatgtaaa agtcaacgat agtgattatg gggccatcgc tgaagctgga ggaaacgata
    30361 agataactat cagaaataca ttgaaggtcg agggggattt tcttgaatat aagaaaatgc
    30421 agactagtat cgacctccca caagaattaa ctattcccga gcatatcttg atcaatggaa
    30481 atcaaattga tgccaaagat atcttgataa ccggaaatcg agtcacgcta aatctgacag
    30541 attattttat tgctggagat acgttaataa tcgaattgga gacaactttg caggaagtaa
    30601 ttcctgaaaa agtactcact atgcattttg aataccttga aaacgggaat ttgattgagc
    30661 aatcgaatga aataaaccta aagatcgcaa agctgaagga gatagatatc catgtttatt
    30721 ataaagataa agacagtcag attgatattg ctgaaatgaa aacaatcact gggaaaatag
    30781 gagagaagta taaagaaact cctattgata ttgaagggta tgtttttgac tcggattcag
    30841 cgaatacaga aggcatcttc tccgaaaata cagaggatat ctatttttat tacagaaaag
    30901 gcaaacttta tctctcaact gctcctcaat atgtcaattt tggaaaacat aaaatatcca
    30961 gtagaccatt aagtgaattc ggacagtttt caggtgggtt gaaaattata gatgaacggg
    31021 cttttggaag ttggaaatta caattgaaac aagagaaagt tcttacaaat ggagattttg
    31081 agcttccaga agctctttcc tttgttacct ctgaaaatac aacggttata gggacatccg
    31141 ctgttacgat tttcgaaagt gatcaaaagg gtgaaagcga tttgtcctct cttttagatt
    31201 ctacagctca tcgtggaatc agaataaata ttcctgtaga aaaccaaaga atcggaacct
    31261 ttgaggggaa actatcttgg atacttgcag atgtcccagg aaattaacag gaagagaaga
    31321 atataacaaa attattgagt ttcaagaagg gcacaggtac cataggctct tgtgtcccac
    31381 agagacaaga ttttgttata gtcttctctt acatatgatt aaaggagcga ttttcttgag
    31441 accaaaacaa caattattta taacactcct agttatttta ggggcacttt ttaacaatat
    31501 atcagtgtca gctagtgcat ttaatttttc agtgacgccg ataacttcag agaatcaaat
    31561 cgacaagaga aaaacttact tcgatcttca acttgatccg gatcaggaag tagaagtaaa
    31621 agcggaattg agaaatgata cagaaaaaga agtgaaaatc gatatttctg tgaatagcgc
    31681 aaccaccaat tcaaatgtga tggttgaata cggaaaaaac gagattgaaa aagacatgag
    31741 cctgatcttt gatctggtag attatgtgag ctatccacag atagtgacgc taaaacctaa
    31801 aagtgtccaa accgttgctt ttcatgtcag gatgccgaat gagcgatttg atggtgtatt
    31861 ggctggtggg atcacattcc aagaaatcca gacggaaaag gaccaaacag aaactaagga
    31921 tcaaagctta tcgattgaga atgaatacac ctatattatt gctttgttga tgcaacagac
    31981 actgaacgaa gttgctccga accttactct tcatgaagtt aaaccagatc aaatcaatgc
    32041 aagaaatgtc atcttagcaa acgttcaaaa tgatcaaaaa acctatatca atcaagtagt
    32101 tattgaaaca aaaattacaa aaaaaggaca ttctgaagtt ttatatcaag aggaaaaaga
    32161 aggattgcag attgcgccga atacaaattt ttcttttcca actgctttaa atggtcaacc
    32221 gctaacccca ggtgaatatc accttacaat gactgtttta ggaaatgaaa atgaaaatgg
    32281 aaaattttcg agaaaaaaag gaaatactac gatcaactat accaatcaat gggtatttga
    32341 aaaagatttt acaatagacg gaaaagtagc caaggaattg aacacgacgg atgttacgat
    32401 taaaaaagac aataattgga tttatatctt agtagcttca cttttcttat tgtttatttt
    32461 attatgcata cttttgtttg ttttgagaaa aaaagaagag aaagaaaagt aaggttaaga
    32521 taacaaatag taaattttta ttattgtagg atggttgggc tctttttata acaatataaa
    32581 atataccatt ttattaataa aaatgcatac tagtaaatga tataattcta ataacaggtt
    32641 tgatcgcgag aaaggccaaa taaaacgctc tccaaaatgt taaaaaaaag acaggacgat
    32701 gtatattata caaacaagaa tcgctgacaa cagtgactcg gatccaggaa atcagtttta
    32761 tccccagcta gttggtgaat gcatggaaag taatatacgt agaaagagct gatgactaat
    32821 gaattatgag ttttttttaa gcaaagaaga tcacagaagt tattcattac ttaaatattt
    32881 agaagcaagc tcaaatttat cagagtcaat atcgaatgtt caagaagagt tgtcgctatc
    32941 gacatttctc ttgaaaaaaa cgatcgataa gctgaaacat gatttacaaa agttcgatct
    33001 agagaaaaat ttgcgagtaa ccgtcagtga attggatata tgtctggaaa ttaatggaaa
    33061 atgttctagc aaagctctgt tatctcgtta tataacggat tctttatcta tgaaaatggt
    33121 attggctttc tttcaggaaa aatatatctc agtagaaaat tttgctaaac agcatcacgt
    33181 gagttattct gtggcttata aagtattaca aagactaaaa agaaatctaa aaaaatatca
    33241 aatttttttt gaaaagagaa agttga
//
LOCUS       DAZBSA010000018        32184 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000018 DAZBSA010000000
VERSION     DAZBSA010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.018"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(122..502)
                     /gene="yajC"
                     /locus_tag="ACHGMT_000801"
     CDS             complement(122..502)
                     /gene="yajC"
                     /locus_tag="ACHGMT_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFN6834570.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(599..1744)
                     /gene="tgt"
                     /locus_tag="ACHGMT_000802"
     CDS             complement(599..1744)
                     /gene="tgt"
                     /locus_tag="ACHGMT_000802"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFN6834571.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1962..2660
                     /locus_tag="ACHGMT_000803"
     CDS             1962..2660
                     /locus_tag="ACHGMT_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFN6834572.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2828..5467)
                     /locus_tag="ACHGMT_000804"
     CDS             complement(2828..5467)
                     /locus_tag="ACHGMT_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFN6834573.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6086..7117)
                     /gene="queA"
                     /locus_tag="ACHGMT_000805"
     CDS             complement(6086..7117)
                     /gene="queA"
                     /locus_tag="ACHGMT_000805"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFN6834574.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7310..8485
                     /locus_tag="ACHGMT_000806"
     CDS             7310..8485
                     /locus_tag="ACHGMT_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFN6834575.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8489..9130
                     /locus_tag="ACHGMT_000807"
     CDS             8489..9130
                     /locus_tag="ACHGMT_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6834576.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9127..10044
                     /locus_tag="ACHGMT_000808"
     CDS             9127..10044
                     /locus_tag="ACHGMT_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN6834577.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10041..10712
                     /locus_tag="ACHGMT_000809"
     CDS             10041..10712
                     /locus_tag="ACHGMT_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6834578.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11253..11741)
                     /locus_tag="ACHGMT_000810"
     CDS             complement(11253..11741)
                     /locus_tag="ACHGMT_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFN6834579.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11942..12208)
                     /locus_tag="ACHGMT_000811"
     CDS             complement(11942..12208)
                     /locus_tag="ACHGMT_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFN6834580.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12208..12471)
                     /locus_tag="ACHGMT_000812"
     CDS             complement(12208..12471)
                     /locus_tag="ACHGMT_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFN6834581.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            12992..13132
                     /locus_tag="ACHGMT_000813"
                     /pseudo
     CDS             12992..13132
                     /locus_tag="ACHGMT_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(13274..15097)
                     /locus_tag="ACHGMT_000814"
     CDS             complement(13274..15097)
                     /locus_tag="ACHGMT_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFN6834582.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15695..18400)
                     /locus_tag="ACHGMT_000815"
     CDS             complement(15695..18400)
                     /locus_tag="ACHGMT_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFN6834583.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18706..19653)
                     /locus_tag="ACHGMT_000816"
     CDS             complement(18706..19653)
                     /locus_tag="ACHGMT_000816"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFN6834584.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19843..20112
                     /gene="rpsN"
                     /locus_tag="ACHGMT_000817"
     CDS             19843..20112
                     /gene="rpsN"
                     /locus_tag="ACHGMT_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFN6834585.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20288..20650)
                     /locus_tag="ACHGMT_000818"
     CDS             complement(20288..20650)
                     /locus_tag="ACHGMT_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFN6834586.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20661..21782)
                     /gene="alr"
                     /locus_tag="ACHGMT_000819"
     CDS             complement(20661..21782)
                     /gene="alr"
                     /locus_tag="ACHGMT_000819"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFN6834587.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21798..22148)
                     /gene="acpS"
                     /locus_tag="ACHGMT_000820"
     CDS             complement(21798..22148)
                     /gene="acpS"
                     /locus_tag="ACHGMT_000820"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFN6834588.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(22278..23789)
                     /locus_tag="ACHGMT_000821"
     CDS             complement(22278..23789)
                     /locus_tag="ACHGMT_000821"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFN6834589.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(24111..25463)
                     /gene="murF"
                     /locus_tag="ACHGMT_000822"
     CDS             complement(24111..25463)
                     /gene="murF"
                     /locus_tag="ACHGMT_000822"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFN6834590.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(25616..26692)
                     /locus_tag="ACHGMT_000823"
     CDS             complement(25616..26692)
                     /locus_tag="ACHGMT_000823"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFN6834591.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            26888..28633
                     /locus_tag="ACHGMT_000824"
     CDS             26888..28633
                     /locus_tag="ACHGMT_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFN6834592.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(28913..29350)
                     /locus_tag="ACHGMT_000825"
     CDS             complement(28913..29350)
                     /locus_tag="ACHGMT_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFN6834593.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(29334..30707)
                     /locus_tag="ACHGMT_000826"
     CDS             complement(29334..30707)
                     /locus_tag="ACHGMT_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834594.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(31029..31517)
                     /locus_tag="ACHGMT_000827"
     CDS             complement(31029..31517)
                     /locus_tag="ACHGMT_000827"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6834595.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(31539..32141)
                     /locus_tag="ACHGMT_000828"
     CDS             complement(31539..32141)
                     /locus_tag="ACHGMT_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFN6834596.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         9200 a         6884 c         5942 g        10158 t
ORIGIN      
        1 gacacacccg attattcaat attgagcaat atatcccatg ctcttatctc tttctttttt
       61 gaataaaaaa tgaggtagga catctttctg ccctacctcg tctttaaata agctttgata
      121 cttatttttc ttccttctct tctgtctctt ccagcgtttc tgttgtttca gtttttactt
      181 caactgtctc agctggttct tctgttgttt caacagtagt cgttgcatca tttgttacga
      241 cagtccctgg tttgaccgtt ttgattgctg cacgatcgta ctcaaggaag atcccttcgc
      301 agtctaaaac aactgtccgt ttgtcattgt cgatttctga aacaactcca tgtaatccgc
      361 cgatcgttac gactgaatca cctggtttca tgctatctaa caagttttga cgttcttgct
      421 gttgtttctt ttgcgaacgt gacataaaaa accacattgc taatagcgcg ataaacatca
      481 caagcattgg taatgttccc atttttttca cctcactaat tctgtatcta caaaaaacac
      541 tttagcagaa aaatgctgat aacactagtt ttttacttaa tttttaaaag attttccttt
      601 agaagctttt tgcgttttct ttattgaacc cgtattcttc aaaaaaggct tctctaaatt
      661 caagcaaatt atcatccatg attgcttgac gtacttgttt cattagattc aacaagaagt
      721 ataaattgtg gtaagaagtc aaacgaatcc caaatgtttc atcacatttg atcaaatggc
      781 ggatataggc acgtgtatag tttttacatg tataacaatc acatttctca tcaattggac
      841 ggaaatcatg ggcatatttc gcatttttca ctactagacg gccttgagaa gtcatacatg
      901 tgccatttcg agcgattcga gtaggcaaga cacagtcaaa catgtctact ccacggataa
      961 caccatcgat caatgaatca gcagcaccga cacccatcaa gtaacgtggt ttgttttcgg
     1021 ggatcaacgg tgtagtgaat tctaatacac gattcatttc tgatttaggt tccccgacag
     1081 ataacccgcc gattgagtaa ccagggaaat ccatcccgac tagatctttc gcgctttgac
     1141 gacgaagatc ttcaaaccct gctccttgga tgattccaaa taatccttga cggtctggat
     1201 tagcatgagc tttcaatcct cgttctgccc atcgagacgt acgttctact gattttttca
     1261 cgtagtcgta actttcatcg aatggtggac attcgtcaaa actcatcatg atatcagagc
     1321 ccaatttatt ttggatgttg attgcttttt ctggtgataa aaacatacgc gaaccgttca
     1381 agtgattttt gaaatgcacg ccttcttctt cgatattccg catatcactt aatgaaaaaa
     1441 cttgaaagcc gcctgaatct gttaaaatcg gttgatccca gttcataaat ttatgcagtc
     1501 cgccagcttc ttctactaag tcttcaccag ggcgtagcca taaatgatat gtattactta
     1561 aaataacgcc agcacccatt tctttcaact cttctgggga cattgttttt actgtagcta
     1621 atgttcctac tggcatgaac attggtgtgg ggaaagttcc gtgtggcgtg atcaattccc
     1681 ctaaacgcgc gccagtatgt ttttctttct tgattaaacg atagcgaatg gcaggttgcg
     1741 tcattcattt ccctactttc tatataaaat atcttgacaa ctcccaccat cataatcgtt
     1801 tcaagtgctt tttgcaagtt ccctcggctt ccttctactt tatttaatat tctttttttt
     1861 atcctttttt tatgaataac aaaaaaaaga aaaacattat gacagaaata ttttttgata
     1921 catggtatat tggtgttatt aaagaaaagg agggaaaaat catgcaatct tctgcagaat
     1981 taaaacgcca agcaaaagag agtctaaaag gacgttgggg acaagctgtt ttactaaatc
     2041 taatccctac attgattggt attgctacta ttcttcttat tatgattcct attatcttgt
     2101 ttgccaccac catatatgat ggatcagcga gcgtctcaga agcgacaact tcaggaggcg
     2161 gtagttcagg aggcggtggc ggaattattt ccagcatcat tagcgctttg ttcatgagcg
     2221 gtatttcttg gacttatctt gatattttac gtggacaaaa agatcagatc gaaccattca
     2281 aagatgcatt tcgtggattc aaaggtttgt ttatcggcgg agtgatcagc ttagctgtgc
     2341 tgatctcgat ctttacaaca ttatggggtt tgctattagt tattcctgga atcattaaaa
     2401 gctattctta ttctcaaagc tattttattt actacgatat cgtccaagaa acaggacaaa
     2461 cacctaaagt actggatacg atcactgcaa gtcgaaaatt gatggatggt tataaaggac
     2521 aacttttctg gttagatctt tcatttatcg gctggcatat cttagcatta gcaactgctg
     2581 gtattggtta tctatggctc agcccataca tttctgcaac aaaagcagca ttttatgaac
     2641 aattgccaaa agatatttaa aagagagtgt gacttgtgac cgagggggtg gcaggagaac
     2701 accgtttatc gagaggcagt aaaagaaaca ttcagcccat tagcaaaaag gaggtgtgac
     2761 aacattcatg tcacacctcc tttttgtata gttccagtaa aaagaaaatc aacttagaaa
     2821 cagttaatca ttcctcttct tctgcttccg gtaatcgatg gaacttgatc agttcattca
     2881 ccgcgattgg tacgatagat aaaatcccta ccaagaacca gtgattcaaa ctgatgtgta
     2941 ctaggccgaa tagttcttgc gtaaatggca gcaaagcaac agccgttgta atagctaatg
     3001 ccagtaacgc catttctacc aaagctttat tggtcgctaa attgactttg aagatcgatt
     3061 ggctgctgcg tacattgaac acatgcagaa tcgatgaata agctaagatc aagaatgcaa
     3121 cagtttgacc gacatgctga cttgcgtcta cccatgggct gatagtatcg acataactac
     3181 ctaaataata accaaacaag gttacgatgg tgaagacccc tgcatttatg gcaatttttt
     3241 gcaataaacc tcgagcaaag atcccttcat ttttcggaat gggtggctga tccatgatgc
     3301 cgtgttccgc tggctctctt cctaaagcaa atcctggaag tccatcagca actacattga
     3361 caaacagtag ttgcacagct gtaaatggtg ctccccatcc taacagcatc gcaatcaaaa
     3421 caataaagat ctccgaaata ttacagctta gcaaaaagtt gatagcttta cgaatattct
     3481 gataaaccga acgtccttga ctgacagcgt ctacgatcgt cgcaaagttg tcatccgtca
     3541 aaatcatatc tgcggcactt tgcgccacat ctgttcctgt gatccccatg gcacacccaa
     3601 cgttacttgc tttcaacgct ggtgcatcat tcacaccatc cccagtcatc gctacgactg
     3661 cacccgtacg ttgccaagat ttgacgatcc ggatcttgtc ttctggcgtt acccgagcat
     3721 agacagagag agctttgaca cgcgcatcta attcttcgtc tgataattga tgcagttctg
     3781 atcctgaaag tgcttctgac ggatcattca agatacctaa ttctttcgct atggcactgg
     3841 cagtcaccac atgatctcca gtgatcatga ctgttttgat gccagccttt ttcgctcgct
     3901 tgattgctcc tttactttct ggacgtggtg gatcgatcat tccgatcaga ccaatcaaac
     3961 gtaagtcttt ttccagtgct tcagaagaaa tatcttgcgg ttcttcgtca taagtagcat
     4021 aaccaacagc aatcactctc agtgcccgtt taccaaagcg atcattaacg atcgccgctt
     4081 gttctacatc gcctgaacta aagcgcggag caagaacatc gaatgctcct ttggtgattg
     4141 agatgtattt ttcgcccatt tggtgaactg ttgtcatcat ttttcgttca gaatcaaaag
     4201 gaagctcgtt tactcgtgga tatttttctt ctaattctgc ctttgtatga taattctctt
     4261 caacagccct tacgatggct acttctgttg gattaccttt gataacaagg tcgtcatctt
     4321 cttgctcgac cgtcacatct gtacatagtg cagccatttt caatatttcc atcgcgctgt
     4381 tcgtcattga atcttcgata ttcgttactt catcttcttt gctccatacc cggcggacgc
     4441 gcatttcatt ttgtgtcagc gttcctgttt tgtctgagca gataacatta gcggttccta
     4501 gcgtttcaac tgctggcaac tgtcggatga tcgcatgttt cctcgccatc ttctgcacac
     4561 cgtatgcaag tgtcaacgta acaataacag tcaaggtttc cggtacagct gcaacagcca
     4621 aggaaatcga agtcatgaac atctcaagga gtggttctcc ttgcagttca ccaatcacaa
     4681 atactaaagc cgcagccgct aaagcgatca tactgattcg tttgcctagc tgattcaatc
     4741 ggacctgtaa aggtgttttt tgcatagtat tttcgttcaa aagatccgcg atcttaccca
     4801 tttctgtagc catgccaatg gcagtgacga tcgctttccc acgacctgca gcaaccgtac
     4861 atcctttaaa taccatatta agttggtcac ctaatgactc atcttcttca gcaataaatg
     4921 ctgcatcttt ttcaactgct tcactttctc cagtcaacgc cgattcttct acacgtaatt
     4981 gtgatgcttc gatcaaacgg gcatcggcag aaatagcatc accggactct aaaacaagaa
     5041 tatcacccac taccagttgc tctgactcaa cagtggtcac tttgccgtct cgtatgacag
     5101 ttgccgtttg tttattcata tcttgcaagg attccagtgc tttttctgcg tttccttctt
     5161 ggacaatcgc caataccgag ttgatcacaa cgatagcgat gatcagcagt ccttcaaata
     5221 aatccccatg ttctgtagca aatgcggtat aaaaggaaat caccgctgct acaagaagga
     5281 tgatcgttgt aaaatcggac aagcttcgaa agaatttttt gatcatcgat tctttcggcg
     5341 cctcatcgaa tttattcaat ccctgctttt ttcgccgttc atccacttct tgtgaagaca
     5401 acccagtatc tggatctgct tctgttacct tctttacttc ttctattgtc tgcttgtatt
     5461 cttccatcct attcctcctt taaaatcttc gtacaaaaaa aggtacttat tttactataa
     5521 catccttttt ttatttactt caaataaagg gcttatcgat tatttgttaa gaaagcgtaa
     5581 aatttaaagt gcttatattt gcaaacgcta ccaaaggatg ctattatata gatgtaagga
     5641 aggaggatac catcaaatag acagaagaat ttctcgatag aacagtgtaa gcaaacaaag
     5701 ttgttagaag tacaggtttc acccagacat gcgtgtagag tacaaaccta taaatagtga
     5761 atgcgtatgg atgtttggat cacgaagtat ccgaaacaag aagtaatgcg atacgtaaca
     5821 attgaataac gtaaacgtct gaaaattgaa taggaacaag agatgtttca aatgttgaag
     5881 atgtaacgaa aacaaaattt ttgtttaaca tgaagaaatt caacaccgtg ctttgcatgt
     5941 atgatggaaa gttacaatca tttctgttta tttgaatcct tccgaggtac aagaaagccg
     6001 agcttatcga gagctcggct ttttctttgt cataaaatct ttgtaaagct tttttgttat
     6061 tcaaaactag cggcttttca taaccttatt tcacgaacat cgcatctcca aaactaaaga
     6121 aacgatagcg ctcttcaatc gcatgctggt aagcagataa agtcaattct cttcctgcaa
     6181 aggcactaac taacatgact aaagtcgatt ttggcaaatg gaaattagtt gagaaagctt
     6241 ctaccacttt gaattcatag ccaggcgtaa tgaaaatatc agtccatccg ctgtctgctt
     6301 tgatttctcc attaaatttc gttccaatcg tttctaatgt tcgaattgaa gttgttccga
     6361 cagccacgat tttcccgcca tttttacgta cttggttcaa tcgctcagcc gcttcttccg
     6421 tcaaacggta gaattcacta tgcatatggt gttcttcaat tttatctacg ctgacaggtc
     6481 ggaatgtacc taaccctaca tgtagtgtca gatagactaa ttcaacaccc tttgcttcga
     6541 tctcatcaag taattctttc gtaaaatgca atccagctgt tggcgctgca gcagaaccat
     6601 tttctttcgc atagacagtt tgatagcgat ctgggtcttc cagacgttct ttgatatatg
     6661 gaggcaatgg catttctccc aatgattcaa gaatctctag aaaaattcct tcatactcaa
     6721 agtcaatgat ccgtccgccg tgttccaatt cttctttcac tactgcttgc aatctgccat
     6781 caccaaaaga gattcttgtc ccaactttgg cacgtttcgc aggtttgatc aatgtctccc
     6841 acgtatctcc ttgtgtattc gttagaagaa ggacttctaa atgcgcacca gtatctggtt
     6901 tttctccata taaacgagca ggcagaactc gtgtattatt cataactagc gcatcaccag
     6961 gctgtaactc ttcaatgatt tcatggaaat gtttatcttc catctctcca gttttccgat
     7021 ccaaaacaag taaacgagaa tgatcgcggt cttttaatgg tgtttgtgcg attaattctt
     7081 ccggcaagtc aaaatcaaaa tcttctgttg ttaacatatc gtcactcact ttctatcatg
     7141 tgcttcatca ttctatcatg tttttcgttc tgttggcaaa atcagcactt tttcacttct
     7201 attttttgtt ttcctaaata aaaaaactac cctatttgta ttttttcatt gttttaggca
     7261 tactagtaat agatagttct tcattgaaaa tagaaaaaga ggtggcacca tgattgaatt
     7321 tcaaaatgtg tcaaaaattt ataaaggtgg aaaagtcgct gtagaggacg tcaatctttc
     7381 atttgaaaag ggtgagttca tttgttttat cggtacgagc ggaagcggaa aaacgacgac
     7441 gatgcggatg atcaaccgaa tgactgatcc aacacaaggg aaaattttga ttgatggaaa
     7501 atccatccaa gaaattgatc cagtggaatt gcgacgtcag atcggctatg ttatccaaag
     7561 tattggattg atgcctcaca tgacgatccg tgaaaatatc actcttgtcc aaaaattgtt
     7621 gaaagtcagt caagaagaac ggaataaaac agctgaaaaa atgattgatc ttgtggaatt
     7681 gccccgtgag atgttagatc gctacccaca tgaattatct ggtggtcagc agcagcgtat
     7741 tggagtcgtt cgtgcccttg cagcgaatca agatatcatc ttgatggacg agccattcgg
     7801 tgcgcttgat ccaatcactc gtgattcctt gcaagacctt gtcaaagatt tacaagagcg
     7861 tttgggaaaa acgatcgtat ttgtaaccca tgacatggat gaagcgatta aactagcaag
     7921 ccgtatcgcc atcatgagtg aaggccgtgt gatccaattt gatacaccag aaaatatttt
     7981 acgtcatcct gcaaacgatt tcgtagaaga gttgattgga gaagatcgcc tactacaagc
     8041 caagccaaat gcgacaagag tcggcgaagt tatgctaaat acagcaatca cgattacacc
     8101 ggaaaaatca ttacaagaag caatcaaact gatgagggaa aaacgtgtag atacgttgtt
     8161 agttaccgat aattcgaatg tattaaaagg atttatcgat gtagaaacgt tgaataaaaa
     8221 acgaggaaaa gtatccagtg tcggcgacat tttgaataaa catgtttttt acgtaaaaga
     8281 atccgcctat cttcgtgata ccttgcaacg cattttaaaa cgcgggctga aatacgtgcc
     8341 ggtcgttgat gaacagaaca aagtcgtagg tatcctgact cgtgcatcat tagtggatat
     8401 cgtctacgat gtgatttggg gagaagatcc cgcaactact gatgttgcag agtcagctac
     8461 tcctgaagaa tctaaggagg tctgacctat gcagcagttt tttcatcagc acggttcaga
     8521 aattctctca aaaggttttg aacatcttta tatttcagga cttgctttga tcattggtat
     8581 catcattgct gtaccgcttg gtgtcttact gacacgtttt ccaaaagctg ccacgattgt
     8641 gattggctta accagtgcct tgcagacaat cccttctctt gctttattgg cattgatgat
     8701 cccgctattt ggcgtaggta aattacctgc gattatcgca ctctttattt attcattatt
     8761 gccgatttta cgtaatacgt acattgggat gaaaaatgtc gattggaatt atcgggatgt
     8821 ggcaaaaggc atggggatga ctgagttcca atccattttt tccgttgaat tgcccattgc
     8881 tatgccaacg atcatggctg gtattcgttt agctgccgtt tacgtgattg catgggctac
     8941 acttgcctct tatatcggcg ctggtggttt aggcgatttg atttttagtg gactgaataa
     9001 ttaccaacca gatctcattt tggcaggtac gatccctgta acaattttag cactattggc
     9061 tgattggctt ctaggtctct tggaacaccg gctgacaccg atcgcactta gagaggagaa
     9121 tgaagaatga aacgtatact aaaaatgacc atggttcttt tctctcttct tatacttgcg
     9181 agttgttcct tccctggtct tgccagcaat acagatgaag acaccatttc gatcacaggt
     9241 ggaatcactt cagaagcaca gatcttagca agtctagttg ccggaatggt tgaacattat
     9301 acagataaaa ataccgcaat catcaacaat ctggctacta caactatcaa ccatcaagcg
     9361 atgatgaacg gagatgcttc aatttcagcg gctcgctaca caggaacaga tttaacgact
     9421 acattgaacc ttcctcctga aaaagatccg aaaaaagcgt ttgctaccgt gaaagatgaa
     9481 tttgagaaaa gatacggtca aacgtggttc ccgtcctata gttttgaaaa tacgtatgtc
     9541 tttcttgttc gaaaagacac cgcacaaaaa taccatctat ctaaagtcag cgatctgaaa
     9601 aatgtagcgg atgaattagt agctggtgta gatacttctt ggatcaatcg taaaggagac
     9661 ggttatgacg gcttccaaga aacttatggc ttctccttca attcgatatt acctatgcag
     9721 attggacttg tttatgacgc agtcgaagca ggaaaaatgg atattgttct tggctactct
     9781 acggatggac ggatcgccag ttatgatcta gtcatgttgg aagatgatcg tcaattcttc
     9841 ccaccatatg atgctgctcc agttgtagac aacaagatat tagctgatac gcctcattta
     9901 aaagaagcct taaataagtt aggtcatacc atcagtacgg aaaaaatgca ggaattgaac
     9961 tacgaagcag ataataatct agtggaacca tcagtagtag cacaacgttt cttacaagaa
    10021 aaccactatt ttgaagggaa gtgaagcaat tggataaaat gaatgtcttt caacaatttt
    10081 tctattattt ccaggaaaat ggctcctata tctttgcaca atttatccgt cacttcctga
    10141 tttctatcta tggtgtcctc tttgcagcag ttgtcgggat tccggttggg atcatgattt
    10201 ctaggagacg taaattagca aactgggtga ttcgtttggc gaatatcatc cagacgatcc
    10261 cttctctagc catgatctcc attttaatca ttggattagg cctgggagtc aatgtcgtga
    10321 ttgttactgt atttttgtat tccctcttac cgatcatcaa gaatacgtat accgggatga
    10381 tccaagtaga caaaaacatc cttgatgtcg gcaaaggcat ggggatgact gctagacaac
    10441 gactattcat ggtagagtta ccactctctg tttctgttat catggcaggc ataagaaacg
    10501 cgttagttgt tgccatcggg ataaccgcga tcggtgcatt cgtcggtgct ggtggtcttg
    10561 gagatatcat tatccgagga accaatgcaa cagagggcac atcgattatt ttagcaggtg
    10621 cattgccaac tgcattaatg gcgatcatca cggactggct tttaggtatt ttggaaagac
    10681 ggctagatcc agccagccgt acatcacgct gatgttattg taaaaaacaa aagcttctgc
    10741 gaccatttgt tggtcgcaaa ggctttttta gttctgtcat ttttaaggca ataatccact
    10801 ctgattcttc cctccttatg ggcaaagagt tgcttttctt agctatttat ccattccgta
    10861 tactccaact tcatactgcc aacgggttca tatctgcatc caccgactaa gtttttttct
    10921 tatccgctaa ggcattttgc caatccattt tcccatcact cgtcatggaa ttcataccac
    10981 atgatctcta aatactcgtc tgcttgttct ttgaattggg catcaattct tacatccttt
    11041 ggcgtggctt tcatttaagc cttttccatc agtcctcgat tgttcgatgc cttcagctgc
    11101 atcatcaaaa ctggtgactg ccgtatcggt aacttcgatc atcttttata caatcgggaa
    11161 cttgccatcc tcatccttgg ctaaaaacat atctagccat tccagcttct cgccatggga
    11221 ggtttgcggt tcctaaaacc ggtacacgat tcttatggac tgcatccgtc gcaactagac
    11281 ttggcgatac gatgatcctt caccagaaga gcacccccgt aaaccaattg atcgatatat
    11341 tgccaataag aaaagacatt ggcgtaaaag atatcgatcc ctcgtgatgc attgccgttg
    11401 atactgctat tcatggccga aagcgccacg actttaactt cttccgtttg agtgacattc
    11461 atgttcggca atgtcgcttt ggatacccgc tctgctaaag gaacgacact ggtattgtat
    11521 ttcgtatctg tatcttttcc aaaagaccat gccaacgaat cctctgggaa ccaataagac
    11581 gactccggtt gattgtccat tgcttttttc aacatacttt tggctgtttc tcgataaacg
    11641 acatttccaa ttgtcttcgc ctcactttgg cgataatcga tggtcgttgc cactgccaac
    11701 actaacaacg atgctccaat tttactgatt ttccttgtca cacttcttcc tccttgtttt
    11761 acataaaccc gtcaacattc cattcctaaa ctcaaacaat gacccttcaa aggtggagat
    11821 ataggaacaa attaaagaaa tcatgcaata aatccaagaa ggttggtttt agaacaaaaa
    11881 aagagtctcc tttttgggga gactctttgt taactatcta aaaaggttgg ctcttctcac
    11941 ttcactcata atgtgtccag gcagaataaa tttctacaac tttattttcc tcatcaactt
    12001 tgtaaaccac tcgatgctgt ctattcagcc tgcgtgagta tctgccttca tgtgtaggtc
    12061 tcagcttttc aaaagactgc gtgggcatat atggatcttc cttcaatgtc tgaatcactt
    12121 cttcaaattg tttctttaaa ttcgtttgct tgatttttct taaatccact ttcgcagagt
    12181 ttttgattgc gactgtatag ttactcatca aagagcttcc caatcaatat catcgatatt
    12241 cgtaaagcta ctgtcgtctt tttcgcggtc tcgtacttta tctaaagtac ctgtttgctc
    12301 aagaaaaagg gtttcttgaa tcgctctcca gtcttccagc ccaatcaata cagcattgtt
    12361 tccactacga tcactaataa tctcgatttc agtatgattt tcatttacct ctttcaataa
    12421 ctgataaaaa tttttacgag cagtgctagg attcatcgtt gtattagcca ttgtctctcc
    12481 tcctttttct gatataatta tagcgtactt gattaagtac gtcaaaaaaa tacaataaag
    12541 cacttaaatt cagcaaactt ttcctctttt ttacatagag aaaaagaccc ctttttaggg
    12601 aggatctttg tccacgctca cttcttactc ctttacgact agcggtagcc aaacgtaaag
    12661 taattggtaa caacgtatca tctttagcaa aggtcgaatc caaagcctgt gagtctatgc
    12721 aacaacacca atacacgaag gttcctcgtt gcagtgtgac ttgattgata tcttgaataa
    12781 cctgaggata cgcacgaaag atttgtaacg gttattcaaa actgattttg gccgtcaagt
    12841 cttctactgc ctcaaaaatg attgcctctt tcgtctcctg tcattcatgt tcactatcga
    12901 tttgatactt gcttagaccg ttgggtttgt gaatcaccaa ttaaaaatgg cactgagtta
    12961 tggtaacaaa aaatggtcac ttgacgaggc tgagattcct acttatgaag acacatggca
    13021 cttaagagtc gaggtaaagg aagaagctga aaatatagga ggctgtacat tatttggcaa
    13081 aggattcgga aattatgatc aagacatcaa aatcaaaatg ttttacagct aaataggaag
    13141 aataataaca tttcatacgc tgttcccatc tttaatggct tatagagggc acctttagct
    13201 gagaataaga caaaacagac cctctagctt caattaagag ctagagggtc tgtttttgat
    13261 tttaaaactt ggtttatttc ttcaaatggt agctataggt gctgacaacg atattttcac
    13321 gccaagacaa tcgcaatctt aaccgcaaac ttgtttggtt atggagaata ttctgccagc
    13381 gtctgtttgg tacaaattga ggaatcaaca cagtagttgt atagttttgt ttcgctgcat
    13441 ttttacttac gatatctaca taacggataa ttggattttc aatcgagcga taagaagagt
    13501 ggacgattga aaaacggaca tctgggaaat gctttttaaa ttctgcttcg atttcttttt
    13561 ctttttcgac atcttcgtct agcgaaacat gcatcgcaac gacataatca ccgatcgaac
    13621 gtgcgtaatt caacgcaccg atatttaccc gtgtcacatt cccgactaat acaagaacgg
    13681 tattcccgtc ataatcatgt agttttgcat cattttctaa acgaagctgt tctgctactt
    13741 ttttataatg atcatggatc ttataaaaaa tgaacataac gattggcata atgatgaaga
    13801 aaggccaaat gtcccctaac cgatagacaa acaaaatcgc aatgattgca tatgagatga
    13861 acgcacccgt aatattggca atcgatttgc ttaaccatct ttttccttct tttttccatt
    13921 tgacgaccat ccctgtttga gataaagcaa atggaatgaa cacgccgata gagtataacg
    13981 gaatcaagcg ttctgttgag ccatggaaaa taaacagcaa tacgatcgaa cctagcgcta
    14041 gtgtgataat cccattggaa tagcctagtc gatccccacg atcttgatac atgtgaggca
    14101 tgaatttatc ttttgccaga ttgtatgcaa gaactggaaa agcagaaaaa ccggtgttag
    14161 cagcaactgc taaaatcagc gctgtagcaa attgaaggac gtaatacaaa ataccatgtc
    14221 caaagacagc tttaccaatt tgagacaaaa cagtgacttc tttttcagga acgatcccat
    14281 accaatagtt gataaatgtg atccccacga agaagaaacc taagatcaat gccatcatcg
    14341 tcaatgtagc agcggcattt tttgctcttg gcttcttaaa gaatggtaca gcattactaa
    14401 tcgcctccac cccagttaaa gaggaagaac cagaagaaaa tgctcgtaaa atcaaagcga
    14461 tagaaacacc cggcacagcc gctcctggaa gtgcagtagc ttgcaatggc acagcacctg
    14521 tcacgatatt atataaacca acaacgatca gtaacgagat cacaaggata aatgtgtaca
    14581 ccggaaataa taggaacgat gcagattccc tcagcccacg aagattcagc atcatcagta
    14641 gcaaaacgat tgtcacagaa atagcgactt gatgaccgta gagtgctggc accgctgatg
    14701 tgatcgcttc agcacctgct gagacagata ctgctactgt caacatgtag tcgatcaata
    14761 aagagccacc ggaaatcaag ccagcattct ttccaagatt ctcactgctg acgacatatg
    14821 ctcccccgcc atgaggataa gcatggatga tttgacgata tgatagcgtt aatgaaatca
    14881 ataaaataat aacaaaagcg gcaatgggta gcgaatacca gatggctgca gcagacaaag
    14941 cgactaaaac gacaacgatt tgttccgtac catatgcaat agatgataac gcatcagacg
    15001 acaataatgc caaagcagca aatcgggtta atttgtgctc atcattttca gcagatttta
    15061 aaggcttacc tactaacaac ctttttaagt aatccaattt ctaaactcct ttacacacaa
    15121 ttttacttca agttttatag tcgaaaattt atagtaaaca agatgttata gtacgctcac
    15181 tgaccttaaa agtcaataaa ctttaagaaa ataattggaa aaaaacagaa aaatatcaag
    15241 cgctttttca aacgctttaa tacagcaaaa atctaacatt tttttatggg ctttttcttt
    15301 gaattctata ataaaaggat agaacttgac ttagaaaatc aagttctatc ctttttgata
    15361 tcttaacttt tggaagagag atgtaaggct gctttctacc catcattctt actactcgaa
    15421 gcatagcgca gttgtcacaa cctcttgata aacggtgttt aaaagcttgc atctgcggta
    15481 ataagcccaa ccaaattaaa aacataagaa gctgttttta tttggtcgtt cttattactt
    15541 gatgcagagc agctttttta caacctcttg gtacacggtg ttcaatcagc tgaccctcgg
    15601 tattagctca aaaaaacggc aaaatatgag gaactatttt tccatttttt tctctaatac
    15661 tcaggtcaaa acactgattt cacaacctct ttttttaatg aaaaatatgg ctctcatgcg
    15721 ccgcttcttt gaatttcgtc attttttctt cgatgaacgg atagattgcc gttcctacga
    15781 ttccaaagac aacaaacaaa ccgatcatga tccagattgc tttcgtagca tttggccaat
    15841 aaattcctcc tgcagattcc cgtaacaagt ttaccgcatg agtaaatggc aagaacggat
    15901 tgatcatttg gaagaatttc cctgagactt ggatcggata gttcccgcct ccgccagaga
    15961 tcgacagtac taagatgata atagcgatcc ctttaccgac attatcgaat aatgcagcca
    16021 agacatagac gatcatcatg aacgctaagg cgatcaagac agcaaatgac acgctgtaga
    16081 ccggattttg gacatctact cctagtaaga aaatattccc taatgtaacg atcaagcttt
    16141 gcgcaatcgc cattgtcaaa aatgtcagca tacgagcaac gaattgttct cgtttcgtaa
    16201 aattgtttcg gtctttcttt cctaaatagt attctgtcgt agcaacactt gaaagaagaa
    16261 cagcgccgac ccacagacat agtgcagtat aaaacggggt gctagctgaa ccgttatttg
    16321 cgattggata aagcgcattg gtttgtagct caacaggttg tgtaaagaag tcactttctt
    16381 ttgttgcgtc tagtttcaat aatttgatga cttcgcctag atcgacttct tgttcgcctt
    16441 ttcggatcgc gtctgctgct ttttggatac cagctttgat atttggccag tcattgttga
    16501 tcaagtccgc agcagcgttc acagctgttt cgacttctgg cattttttca tttactgttg
    16561 tcaatgtacc ggtgatttca ttttttagtg ctggccaatc attttggata aaatcagcgg
    16621 ccaatcctaa tttttgctga atgatcggta attcattgtt gtataggtct gccccttcat
    16681 tgatgccatt gacgatggta tccatatttc cattcagcat tgtattggca tcatggattt
    16741 cttgtccaat tgcaggcatt tctttttgat atttttctaa aattgccact gcattcgtta
    16801 cagttgcttt cgttgagctg agaagcgaat caaagtcgat ctggtccgct ttgctcagtg
    16861 cgccttgtgc agtcccaatc gtatcgatga cttggtctag tagagaagtg acagtggaac
    16921 tgatttgacc tgtattgata ccagcaatga tgccattgat attattaaca acggcatcaa
    16981 tttgatctag caattcatca atagccttcg tgtcttcatt ttgaacagct gtgttcaaag
    17041 cagataggcg gttttgcaga ttgcttagca agggttttaa ttgatttagc tgatcaataa
    17101 ttggctgtaa tttcgtattt ccagcacttg tttgcaattt agtcaggaac tcgaccagtt
    17161 tatcgattgc attttgttgt tgagatattc cgtcaataaa gccgttcaat acattgctga
    17221 tgtttttctt ttcatcatca gtcagcttac catcttcaat agctgttctt acagtatcta
    17281 ttaacgaacc tgtcgttgtt gccacttgtc caattgactt caatgtaaca tctacactgt
    17341 ttgtgatgct tggtatcgca tctttcagct gagaggcacc tgattttgtt tggtttgcta
    17401 gatcacttgc ctgattttct aaatttttca catctggcaa gatatcttgt acttgttgga
    17461 tgatcgtcaa gccttgtttt gcttcatcga ttccttggtt cattgtctct tggatcgaag
    17521 caaaatcgcc gtcgatctct gctagttgct taccggcatt ttggatctct gggatttttt
    17581 cttgcaaatc aaggatgatt tttgcttgat ctttcaactc aggcattttg tcattcaatg
    17641 caacgacttt ttcacccatc tcatctactt tagggatgta atccacgaat tcatttgctt
    17701 tattcagttt ttccttgatt tcaggtagtt gactttgtaa ttcgaggact tgttttgtgt
    17761 agccgtcgat cgtgtccaaa ttttcatctg ttgataaaat catatcttta actttattga
    17821 tacttactag gttcgtatcg atgtcgtaac ctatctcatt gaatactttc aataaagtcg
    17881 aactggctgt tttgataaat tcatttgtga tttgcgattg gatcgaactt gctcctttat
    17941 cggtgatttt cggtgcaatc gcattgattt tttcatttac cgtatattcg atttttggtt
    18001 tcttgatatc accactagta aaactcagca gatcttctga gaaatccttt ggcaagtaaa
    18061 tacctgcgta atactttccg gaacggactc cgtcttctaa ttgttctttt gaatcaacga
    18121 attgccatcc taattgttta tttttatgaa gcgattcgat gacttgctcc ccaatggcca
    18181 cttctttacc ttgaaactct gctggcttgt ctgcactgta aacagcaatt ggcagctctc
    18241 cagtatttcc atatggatcc cataatgctt tgatgttaaa ccacgcatat agcgaaggga
    18301 tgatcatgat cgcgatgatc agaagtgttg caatcgggtt tttgaaaatc cgtttccagt
    18361 caagcaaata taatcgaagc gtatttttga ttgactgcat agttctcccc cttcttcttt
    18421 tttcagctga attactttac catgtatttc atactttttt caatcggctt ttttcaaaaa
    18481 agtcgaattt ttggacatat cacggcaatt gtctcattgt aatctcttac atattgagaa
    18541 ataattaagg attacagcct ttactttatc atgaaataaa agggaaagat aattttatgc
    18601 caaagttttt atctattata cgtatacgca aaaaagctaa aacatttttt gtctttgccc
    18661 ctacaaaaat gtttcagctt ttttcgtttt ttattcctgt atttcttatt tttcgaatga
    18721 caggatgatt ttcccagcag gaggatgtcc ttcaactagc tgatgtgctt ggatgacatg
    18781 cttaagtgaa gcagacagat tcattgaaat aaaaacatga aaggctcctt tttggtaggc
    18841 tttgattatg ccagcaaagg cttctgcatc tagatattcc tttcttggaa caaacgattc
    18901 ataaaagcct tccttttttt gccgcaaatc tagatctggg aggtcattta atgcaacata
    18961 cctgccgcct ggtttcatga tctggatgcc ggtttctcct ttgatacttc ctttcgtcgc
    19021 atcgattaca aggtctgcct gatcagcaaa ttgcagacct ggatttgttt tatcataagc
    19081 agcaaatgct gaagctccta atttttttac cttttcctca tttttactgg atgcactggt
    19141 taagattcgg attccctttt catggagaag ctggatcaga cttgacccga ccgcacctga
    19201 tgctcctagt atcattacag tatctgtcgg ctggatttcc gtcaaatggt tgattagatt
    19261 ataagccgta attcctggcg tgaccattcc ggctgcttct tcccaactca ttttatctgg
    19321 aatttttgct acttttgctg atggcaacac gacttcttct ccgtaagtcc cgccgactgc
    19381 atggacagct actcgatcac ctacatgaac agttgtgaca tctgaaccaa tctctgtcac
    19441 aataccagcg ccatcattac ctggtacata agggaatttc aatgttcgta tctctttcat
    19501 ttctcctaaa cgcaaggcaa catcataagg attcacactg aatgctttta tttctacacg
    19561 aacatgtcca gaaccgactt ctctgggatg agcatcgatt tcttcaaaaa catcttgtgc
    19621 ttctccgtaa ccgttaattg caaatcttcg catggtcacc atctcccttt ttctttaagt
    19681 ttacgtttat gcctttattt tgacaaaaaa aatgtctttt cgttttggcg catgattcaa
    19741 aacaaaatag ttgtcttttt tcgcgtaacg tgttaaatta tttatctaaa agcgtaaaga
    19801 ttacgattta cgctaataaa aaataattgg aggaatttgt tgatggctaa aaaatcaaag
    19861 attgccaaag caaaaaaaca gcaagaaacg attgaacgct atgctgcttt acgccaggaa
    19921 ttaaaagcgc aaaaagatta cgaaggcttg cgtaaacttc ctttagatgc tcgtccagaa
    19981 cgattgaaaa atcgtgattt aatcgatgga cgccctcgcg gatacatgcg taaattcggg
    20041 atgtcccgta taaaattccg tgaattagca cataaaggtt tgattccagg agtaaaaaaa
    20101 gccagctggt aatccaagtc tggctgtaat gtaaaggagg agggcgcatg gaccactcct
    20161 tggatcatca aaaaagatac gttcgcttct tctagcagaa atgctggtac tagaagcaga
    20221 cgtatctttt tttacgcaca gctggtcctt ttttatatgt aaaggctgca tggttacttc
    20281 gctatattta tttagtaaca ggagtcagtg cttcgatacc cacactaatt ttcaatgctc
    20341 ggtcaacttc tgccataatg ggtcgatcca aatggcagac cttttctttc aatcttgatt
    20401 tatcgatcgt tctgatctgc tccaacagga taacggagtc tctttctatt cctgttccag
    20461 tagctttgat cccaatgtga gtcggaagct taggtttagc catttttgcc gttatggccg
    20521 caacaatgat cgtcgggcta aaatggttac ctagattatt ttgaataacc aaaactgggc
    20581 gtgttccgcc ttgctccgat cccacgactg gagaaagatc agcaaaataa atatctcctc
    20641 tttttaccat aggatcctcc ttattcttga tattctcttg gtatccgctg tcctaatccg
    20701 caagccactt catagtggat cgtacctaat tgatctgcta tatcctgcat agtgatttct
    20761 ttgtctgcat ttttgccgat aagcgtcact tttgttccga caggaaattc ttgaggcaat
    20821 cggatcatca gttgatccat acatacacgc ccaatcgttt cacagtagtt cccttctacc
    20881 aatagagaaa aaccctgcat ctttcgtggc cagccatctg cataaccgat cggtatcgtt
    20941 ccgatccact ctgcttcagg agtgatatat gtttctccat agccgattcc ttcacctgct
    21001 gggagttttt tgacttgaat caattcagaa accagctcaa gtgctggctg caatggataa
    21061 acttccggta gtgcgtgccc agagggatta agtccgtaca ttgctactcc ataacgaatc
    21121 atattgccga ttgtttcatc atgccataaa gccgttgcac tattgctgac atgtacatat
    21181 cgaggaagtt cgggaagttt tttcagcact tctataaagc gttctttttg cagattccaa
    21241 tatgtatcat ctgcctgatc tgctgtagaa aaatgggtaa ataaaccttc ccattctaat
    21301 acacgacttt ctttgatgaa tgcagctgcc tcttttactt cttcaggtgt acaaaatccg
    21361 atacgaccca tcccagtgtc tgcttttaga tgaatcgaaa gaggcgtctc tagcttactt
    21421 ttctctaaaa cagcagctgc tgcttgcaac cattcccgtg tacctgcagt tactgataaa
    21481 tcatgagcaa cagctacagt cagtgcgtcg taaggaacca tattcaagat aaggatgggc
    21541 tgagtaaatc cagcttctct caactcgact ccttcatcta aatttgaaac acaaaagccc
    21601 gaagctccac ctgcaactgc tgcctcagct gtttcaatag ctccatgacc gtatccgttc
    21661 gcttttacta ctgcaaacaa ttcttttcct tgcggaagtc ttgcaacttc atttgctaca
    21721 ttttcagtta tagctttttt atgtatcact gctttcgttg ggcggtgcca agcgacaacc
    21781 atcgtttttc cttctttcta gtcttccaaa ataatttgtg caaaagctgt ttcatttgta
    21841 tgagtaatac tgacccatac attgccttca tgaggtgaac gcgtcacaac aggctgacca
    21901 ttttcatttt ttaaaatctc gacatcttga aaggtaactt ttccgatccc agttccccaa
    21961 gctttagaga aagcctcttt gcaagcgtaa cgcccgccta aaaattctac ttggcgatga
    22021 aaaggcaagg actggaatag tttcatttca tcagaggtca agattcgtgc aatgaatttt
    22081 ggtttttctt cgatcagccg agtgatccta ggaagttcga ctgcatcgat cccaattcct
    22141 tttatcatat gcttttctct tttcaataaa tttagaacac tctcattcta ccaaattata
    22201 accattttgc cacaaataag tccacaaata acaaaaatga aagtttctct taaaaaagag
    22261 aaactttcat tttatgttta atcgttattt gaacggatta caaatccgcg ttttttatca
    22321 ttgtgacgtt taccgccatc tttttggctg ttattatttt tgctataacc gccttttgat
    22381 ttgtttccac gataattacc gccgtcacga cgattacggt tgtttccgcc accacgtttg
    22441 ccatttttat tgtatccttt tttctgcatt ggcaatgggc gttctggtgt gatcttcact
    22501 ggcactgcat cagctggatc ttttgctgtc gtttttagca aaagtgcaac taaatcttgt
    22561 gcagaatatt cttctaataa tttatctgct gtttgcaggt atttatctag accattttct
    22621 gcaagttttg tttcgatttg ttcaactgca gcacctaatt gacctttgaa tgcttctttt
    22681 tcggttggtg gacgtaaagt cgtcatccgt tttttcgtca agttttcaat cacatgcaag
    22741 tagtccattt catttggtgt aacgaaagtc acagacatac cgccttttcc ggcacggcct
    22801 gtacgtccga tacggtgaac gtagctttct ggatcttgag ggatatcata gttgtacaca
    22861 tgagtgacac ctgaaatatc taatccacgt gcagcaacgt ctgtcgctac aaggatatct
    22921 aaatgaccgc tcttaaatga acgcaatacg ctcattcgtt tttgttgaga caaatcgcca
    22981 tggattcctt ctgcacggta accgcgtgct tctaatccgc gtgccaattc gtctacacgg
    23041 cgttttgtac gtccgaagac gattgttaat tcaggtgttt gaacatcaaa caagcgagtc
    23101 atgatatcga atttttcgta ttcttttgcg cggacatagt attgatcgat caagtcagct
    23161 gtcatttctt tcgctttgat cttcacatga tgtggatttt tcatgaattt aacgccaata
    23221 ttcttgatag ctggcggcat cgttgctgag aaaagtaatg tttgtctttg atcaggaact
    23281 tgtgaaatga ttttttcgat atcttccaag aagcccatat tcaacatttc atctgcttca
    23341 tccaaaacta atgtttgaac ggttccaagt ttcaatgtgt ggcggttaat gtggtcaagc
    23401 atacgtcctg gtgtacccac aacgatatgt ggacgatctt tcaagccacg gatctgacgg
    23461 ccaatatctg ctccgccgta aacggcttgt acacgtactt ttttgtcgcg ccctaaacga
    23521 tataattctt cttgtgtttg gatagctaat tcacgtgttg gtgcgataac taaaccttgt
    23581 aattcgtgac gatctggatc gattttttct aacattggta atccaaatgc agctgttttc
    23641 cctgtacctg tctgtgcttg tccaataacg tcttttccag ctaatgctag tggaatcgtt
    23701 tctgcttgga taggcgttgc ttcttcaaaa ccagcgcgtt cgactgattt caacaattct
    23761 ggtgataatt ctagttcttt aaatttcaaa tgaatcctcc tatatggttg ttaactgagg
    23821 atgtagcttt tttgctaccc cgtcgatttg ttcggtcaaa gtgtattgtt tctttttgac
    23881 agtggccacg cacgttttta tccattctta gcccctgtgg caccaccgaa aaatcacttt
    23941 gcgaagttta ggcattagaa aagccaaacg ggtcgcgttc agctttatca ttattatgac
    24001 atctggcttc aacaagttgt ttctatggca aaaccagtcc aatttctctt catctttcgg
    24061 caataggtta ttctaacata gtttttcgtt ttctgcaagt ttacagattt ttatttcatt
    24121 tgctgaagtt gttctaccac ttcaaataag cccatgctgt tgctgccttt tagcataata
    24181 ctatctgttg cttcaagatt ttgtttgata gcatcaatca atttattctt ttctttttca
    24241 aaatagaaca caggtagttg ggggtatttt tggttcaatt cttcttttag tgcatgcatt
    24301 tgttccccat aaaggaaaat catggcgaat ttctcaggat cgatatgttc actcatttgc
    24361 gcatgcatat ttaaagactc tttccctaat tccagcatat ctgctaacac agcgattctg
    24421 cgtccttttg ttggcagatt tttaaaagta tctaatacca gtcccatggc tgttggattt
    24481 gcattgtaga catcgctcaa aatagctgca ccatttcctg ccgttagcca ttcggttctg
    24541 ttttgagtga tagaaacatg ggcgagccct tggcgtatct caggtatgga cagtccaaag
    24601 aagcgcccga tcgtatacgc aatcaaagca ttcgttacat tataacttcc aggcagcggg
    24661 atcgtaaatg tttctccttc tactacaaaa tcggtttgtg cttttccttt tgctgtcact
    24721 tccgcatgaa catcgccatt tccaataccg aatgttgtga ctgtttgtac taatgttgcc
    24781 gttaaaggtg tcagcaacgg ttcatctgcg ggcacaacga gtaatccgtc tgatacaagt
    24841 ccgtcagtga tttccatttt ggctttagcg attccttctc gcgacccaag attttctatg
    24901 tgtgcttcac cgatcattgt gattgctgca gcttttggtt gagccataag tgaaagttcc
    24961 gtgatttcat tggcgtgatc catgcccatt tccaaaacga tcttttctgt atcttggggc
    25021 atgtgcaaaa tcgtgtaagg aagaccaata ttattgttgt aatttccttg cgtcttatat
    25081 gttttgtatt tttcagacaa gacagacgcg gttagatctt tcgtggtggt tttaccattg
    25141 cttcctgtaa ttgcaacgac ttctgcgcct actttttcca agtaataggt tgccaattgc
    25201 tgcatggctt tcaaaggatc tgctacagga ataactggaa aattttttgg agcttgttca
    25261 acaggcaagc tccaaaatga agcagatgct ccgtttgtga tcgcttgctg gataaattca
    25321 tgtccatctc gcgtaccagc aagaggaacg aataaacttc cttcggttat cttccggctg
    25381 tcaaattcga cattggtaat ctttgtcagt gcagagcatt cgctgcccac ggcttgtgca
    25441 atttcttgta ttgttagatt cattgagatc tctcctaaaa aaggttgtga aaaaagcgtt
    25501 tggcctaaac ggcgggttcg catgactctc acttccaagt aaatttctct attcgtattt
    25561 agaagctaga atgaacgcgg ttacgcaatc actccagcct cttctttatt tctctttatt
    25621 catttttttc aaaaaaagat tgacgctgat ggtatcgatt cattcctaac tggatcagtt
    25681 cttcaatcaa atcaccgtat ttcaagccca tattttccca taagagtggg tacatactga
    25741 actccgtaaa tcctggcata gagtttaatt cattcaggaa taattcattt ttatttgtca
    25801 aaaagaaatc gcaccggctc aatccgcttc cacctaacat cgtgtaagct aacttcgcgt
    25861 actcttgcgc tttttgataa acttcttctg gcacttcggc tggaatctgc atttcgattt
    25921 tattattgat atattttgct tcataatcat agaatgctac gtcttttacg acttcaccag
    25981 gcaaagtcgt ccgaacgtct tcatttccta atacagcaac ttcgatttcg cgcgcttcaa
    26041 ttccttgttc aacgattgct cgagaatcat actgataggc tgttgctaaa gcattttgca
    26101 gctcttctcg gttttctgct tttgtaatgc cgacactaga acccatattc gccggtttga
    26161 caaacatcgg ataaagcaaa gaaccttcac attgatcaaa tactttttta ggattttctt
    26221 tccattgatt cttaagtact ggtacataag gaacttgcgg cacaccagca gcttgtaaaa
    26281 tatacttggt catgatttta tccatggcac atgcactggt caatacgcct gcgccgacat
    26341 aaggcatatt caatgtctct aagaagcctt ggatcgttcc atcttccccg tttggcccat
    26401 gtaaaactgg aaaaacgatg gctccttctt ctttgatttc gcccggattg atcacttttc
    26461 ctgtaaagcc ttcctctgtc tgtccacttg ggtcccatga aagatgcaag acatctttgc
    26521 tggcaggttt ttctgttagt aatggacctt tgacccattg tccttctttt gtaataaata
    26581 cgagttgaac ttggtaataa ttataataaa tggcatttaa aactgaaaat gcggaaagaa
    26641 tggacacttc atgctctgcg ctgcgtccgc catatagtaa agtaatcttc aaaagattgt
    26701 cctcctgtat tctaaatcgt tcagtgattt actctcatat tttagcacaa aacaaaaata
    26761 aacgaataac ttatttataa aaaaacgagc cagcttctaa aagaacgagc gtcatttatt
    26821 ttttgtcagc tagattattt gtcccgtttt ttataaaaag ttatgataaa gaaaaggagg
    26881 gatgatgatg gcgcaaacaa atccacgcag ccgcttggca gaaatcattc gagtgttcat
    26941 caattacaat gtagtgccaa actttgtaca gcaaaaaaat cccgaacagg taaaaaaagc
    27001 ctttgaagaa ctaggcccca ccttcatcaa gataggacag atgctttctg ttcgagaaga
    27061 tttgctttct tctgccttca ctcaaacatt caaaacatta caagatagtg tacctagcga
    27121 cacattttca acggtcaaaa agacgatcga aacagaactt tctctttcat tatcagatat
    27181 attcgatgat ttttcaaaat cgccttttgc ctctgcttct atggggcagg ctcatcgtgc
    27241 caagctgaag aacggtgacg cggtagtcgt taagatccaa caccctaaca tcgccgaaga
    27301 gatccgactt gatcttcaac tatttgaacg agcgatccct ttgatcaaat acattcctga
    27361 aactagcgta gttgatctaa aaggggtact ccaagaagta aaacgttcct tgatcaatga
    27421 aatggacttc ttaaaagaaa gtcaaaatgg cgaacaattc tatcaaaaga acaatggctg
    27481 gaaagagatc cgcagcccca agatttatga cgctttttgt tcgaaaaaag tgatcgtgat
    27541 ggaagaaatg tctggtaaaa acttgaatca tttaatgaac gccgaaaaca aaactgaaac
    27601 ttttatcact ggaattcaaa acaagcagct gaaacaagaa gttgcaaagc ttttagttga
    27661 gaactttatg aaacaggtgt ttgatgatgg ttttttccat gctgatcctc atccagggaa
    27721 tctgctattc catgtactaa caaaagaaga acagacacaa gctagccgaa aaactgaaac
    27781 agtccatgaa aaagagttcg gttcctttgc ttttcgagcc tccacttctg cagaagaccc
    27841 tgttgcgcca tataccatca actacatcga ttttggtatg atggggcatc tttctgcagg
    27901 acttaggcaa aaactcacac aagccgtttt agcgctgtat acgaaagatg cttatcgaat
    27961 cgaaaaagcg gtccttcgac tttgtcagca agaaggctcg tttgatgaaa gtcggttcca
    28021 ccaagaactg acaagttttc ttgaacagta ttatgattct cctattgatg aaatcaactt
    28081 gcaagaagta ttcacacatg tcgtgacgat ttgtcatcaa aataatctgc agttcgatcg
    28141 tgatatcacg cttttgctga aagcatttgg gacattagaa ggggtgatcc gcgtcctcga
    28201 tcctgaagtt tccctcatgg aagtcgcttc gccttttgct caacattatt ttctcactca
    28261 tctggatgta gaagatacat tgaaacaaag cggattagac ttattggaag ggatgaaggc
    28321 tgcgcctaag atccctcaac agctccacca tctattagaa atgtggactt cgggacaagg
    28381 aaaagtcaat ttggaattga aaaagcaaga caaattattg agcaggatcg aaagtatgat
    28441 caatcggctt gttttcggta tgattttagc tgcattgatc gtagggtcct ccctgcttgt
    28501 ccaagcagca cctgtagaaa acgcagaggt cgtttcctta ttagggatat ttacgtatgc
    28561 tattgccgca ttcgtcatta tttttttggc aatcgatgca ttgatccaaa tgtataagaa
    28621 gaggaaaaag tagaaaaagg ttgtgacaga aagaatcagc ttcaagaaat aagaaggggt
    28681 tttacataaa ttgtttctca aatttttgtg aaaattcggc ttatttttcg aagaggctac
    28741 ttctgaaaca ccgagaataa aaaatgtgaa aaagctgttc tgcacccagt aataagaaca
    28801 atcaaaaagg gaacatggaa cacatttatc ctgtttccct gttccctttt tgatgtattc
    28861 gagcataaat aaaatacctt cgttacctac tatcttctgt acttggtaaa ccttagtcaa
    28921 ttgccttttt taacgcttga cgaaaagaca tatgatcttt ctccgtaaag ggttttggac
    28981 cctttgtcct tatgccagct tttcttaact ttccactatg ataacgttgt tctagaagcg
    29041 tgccgatatt cgcttcgtca aattcttttc cagagtgatg gaacactcgc acacccttcg
    29101 gcaatacaag tgaagctaaa ccataatctt gcgtgatcaa aaagtcattc ttcttaacca
    29161 taccgacgat tttataatcc gcactatcag ctccgcggtc tacgtagacg atttggacat
    29221 gattagcgta ttctttggtc gtataatggt ccacactggt cacgataacc acgtcaagac
    29281 cataggattc tcctaatttt atgacatctt cttttactgg agaaccatct ccatcaatga
    29341 atagcctcat tctttttcgc cttcttccat aaatgataaa cagttactaa tccgctcagt
    29401 aaaagaccaa cattgatcca ttttgccaag ataagcagtt gtacactttc tatcggcact
    29461 gattcatggt tcagcgtgat caataataga tggattggct ctcccatttc ttttgctaaa
    29521 acaaacaaaa gcgtcatcaa taaaaactgg tggatcacac tccctttatt ttgagtagta
    29581 tacttgacaa cgatccggcg atccattgga atagctgtat cagtttgata atattcctta
    29641 tttagccagc tgcctgttac tacgtgccag aaactaagca gaaagacacc tatcgcaaaa
    29701 ccatttggaa tcaagaggac caataaagaa gaactcagtc caagcaattg catagccaat
    29761 ggagaccatg ttaaacgttt caaaatccat ggtccgacta tttttgctgc aatcaagcct
    29821 gctgcataag gcaaatacat aaaaatcgaa agtttttcta atccgtacag tatgccaaca
    29881 taaaaactgc taaataaaaa aaggaaattc cctagcattc catttagaaa agtaaacaga
    29941 tttagccagc ttggcaactt ccattccttc ttctggtgtc ctaaaacaaa cacgaacaag
    30001 atgaacagca gcaagaaacc ataaatagca agatcaaaaa gcctttcatc gaataacaat
    30061 cgggcatttc tcaaaagaaa caataagcca aagaatatca aaaataaaac aagctctcgc
    30121 gtagctacca gatttctttt gatgtctttg aaatccagtt cgtaacgcgg ataatgacta
    30181 accgtatggt aggccatgac gtaaaaaagt gtataaatag taaagcttgc tgcaatctga
    30241 gtcggtgcag gcaactcaat cgcttgatac aggagaaata gccagatagc tgcaaataag
    30301 tactttttcc catccatatt gataaaacct tgcgtctttt cgtgaaaatt gaccgtggta
    30361 ttagcaggta caagccatgc tgcactcagc cctagcaaaa cacttgaaaa ataaaataaa
    30421 ggaaaataaa atacgcctaa tattccaagc aatgaaccag caccaccaat cagcagagcg
    30481 atcatcagca acgtgtaact atctagacca aatcgtagac tgttgactaa aaatagtcca
    30541 gtcatccgaa aggcataaaa cagcgtaaat ggcaaaatcg tcagccaatt atgcgagtct
    30601 gttagtccca aaaataaaag ataaggaata aaaataaccg tgttacttaa gataaagggc
    30661 gttgtttgtg taaaattacg cccaaatcgt ttgagttttt cgaacatgac aatggctcct
    30721 taaacagtct tataactaat ctgctcttcg attttagcat taaaggggaa ataaaccaat
    30781 tttacagaat atattcttgt aaaaaaaaat acagaaagaa gcataagctt ctttctgcat
    30841 ttttacggta cttgattact caagcagaca agcccttctc cacaaaagtc ttgcccgaca
    30901 cccctgcatc cggcaggtga taaagataga aatcaattat cctgtgcctc caaaatcaat
    30961 taggagctct gtaaaccaga gtccttctcg acccgtcgca taaatttact ttaacacagt
    31021 tcctatgttt atgcgagagc ttttttcatt ttataatcga ccatttgaaa tccttgaccg
    31081 atatctgaaa taagttcttc tgtaatctca aatccttcat gcttataaac ctcgattgct
    31141 tgatgattcc cctgattcac tcgcaaaaag atctctcttt cctttgtttg cgcagcaagt
    31201 tcttcatacc agcgaaaaac tgaagaagtc aatccttgac cacgtacttt atttagcaaa
    31261 tataatttac ttaaataaat ttttccattt atctcttcat aagcagtgta accgacagtc
    31321 ttttcttcgc tttttaataa aaagtaggac acaccttctg tcagttcttt ttgaatctgt
    31381 ttttttgact gataagtttt cagcatgtaa tccacttgtt tttgaccaat gatcggcaca
    31441 aaaacttccg gccatacttc ttgtatgact gccatcagct catccagttc attttctgtt
    31501 acttttattt tttccataca cccacgtcct tttttacatt ataaatgacc ttgcgcttga
    31561 tggaataaaa agagcatgat catgctatac acacccaccg accacggttt caagaaaatc
    31621 acaatctgca taaattccct aaaagataat ttcgtcaaac cagctactag acagatcaag
    31681 tcgtctggcg caaagggaag cagcaaggta acaactagta cttttttaat atctttcgtt
    31741 tttcttgtca gcaattcatc aaatttctga tacttttttg gcgataaaat aagctggaca
    31801 aaggctacgc catagtaacg gacaagcaaa aatccgattg cttccccaat cactaggcca
    31861 atacaactat ataaaagccc aaatccattg ccaaacatca gcatgcccgc tactgaggag
    31921 atccctcccg gcaaaatagg aatgatcact tggatcactt gcaacagaac aaatatcatc
    31981 actgcatact tgccaaattg ctggataaac gcttgcaaag acgtgctcga tcgaaaaatc
    32041 ccatgatgat aagcataaag aatcaacaca aagaaaaaaa acagaccaat aatgggcatg
    32101 atctgaagac atttttgtaa aactgatcct tttttagaca tctatattcc ccacatcttt
    32161 tttcatttat taaaactgcc tcct
//
LOCUS       DAZBSA010000019        31952 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000019 DAZBSA010000000
VERSION     DAZBSA010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.019"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            96..1061
                     /locus_tag="ACHGMT_000829"
     CDS             96..1061
                     /locus_tag="ACHGMT_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFN6834597.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(1118..1348)
                     /locus_tag="ACHGMT_000830"
     CDS             complement(1118..1348)
                     /locus_tag="ACHGMT_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFN6834598.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(1434..2321)
                     /locus_tag="ACHGMT_000831"
     CDS             complement(1434..2321)
                     /locus_tag="ACHGMT_000831"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFN6834599.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(2410..3633)
                     /locus_tag="ACHGMT_000832"
     CDS             complement(2410..3633)
                     /locus_tag="ACHGMT_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFN6834600.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(3650..4696)
                     /locus_tag="ACHGMT_000833"
     CDS             complement(3650..4696)
                     /locus_tag="ACHGMT_000833"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFN6834601.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(4860..6587)
                     /gene="ptsP"
                     /locus_tag="ACHGMT_000834"
     CDS             complement(4860..6587)
                     /gene="ptsP"
                     /locus_tag="ACHGMT_000834"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFN6834602.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(6587..6853)
                     /locus_tag="ACHGMT_000835"
     CDS             complement(6587..6853)
                     /locus_tag="ACHGMT_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFN6834603.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            7332..9566
                     /locus_tag="ACHGMT_000836"
     CDS             7332..9566
                     /locus_tag="ACHGMT_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN6834604.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            9714..10034
                     /locus_tag="ACHGMT_000837"
     CDS             9714..10034
                     /locus_tag="ACHGMT_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFN6834605.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(10239..10463)
                     /locus_tag="ACHGMT_000838"
     CDS             complement(10239..10463)
                     /locus_tag="ACHGMT_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFN6834606.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(10661..12241)
                     /locus_tag="ACHGMT_000839"
     CDS             complement(10661..12241)
                     /locus_tag="ACHGMT_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFN6834607.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(12642..14018)
                     /locus_tag="ACHGMT_000840"
     CDS             complement(12642..14018)
                     /locus_tag="ACHGMT_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFN6834608.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(14146..15234)
                     /locus_tag="ACHGMT_000841"
     CDS             complement(14146..15234)
                     /locus_tag="ACHGMT_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFN6834609.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            15406..15840
                     /locus_tag="ACHGMT_000842"
     CDS             15406..15840
                     /locus_tag="ACHGMT_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6834610.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(15918..16451)
                     /locus_tag="ACHGMT_000843"
     CDS             complement(15918..16451)
                     /locus_tag="ACHGMT_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFN6834611.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(16545..17723)
                     /gene="mutY"
                     /locus_tag="ACHGMT_000844"
     CDS             complement(16545..17723)
                     /gene="mutY"
                     /locus_tag="ACHGMT_000844"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFN6834612.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(17716..18513)
                     /gene="recX"
                     /locus_tag="ACHGMT_000845"
     CDS             complement(17716..18513)
                     /gene="recX"
                     /locus_tag="ACHGMT_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFN6834613.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            18620..20002
                     /gene="rlmD"
                     /locus_tag="ACHGMT_000846"
     CDS             18620..20002
                     /gene="rlmD"
                     /locus_tag="ACHGMT_000846"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFN6834614.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(20176..20754)
                     /locus_tag="ACHGMT_000847"
     CDS             complement(20176..20754)
                     /locus_tag="ACHGMT_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFN6834615.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            21076..21546
                     /locus_tag="ACHGMT_000848"
     CDS             21076..21546
                     /locus_tag="ACHGMT_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFN6834616.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(21654..22880)
                     /locus_tag="ACHGMT_000849"
     CDS             complement(21654..22880)
                     /locus_tag="ACHGMT_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFN6834617.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(23634..23834)
                     /locus_tag="ACHGMT_000850"
     CDS             complement(23634..23834)
                     /locus_tag="ACHGMT_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN6834618.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(23968..24576)
                     /locus_tag="ACHGMT_000851"
     CDS             complement(23968..24576)
                     /locus_tag="ACHGMT_000851"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFN6834619.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(24661..25131)
                     /locus_tag="ACHGMT_000852"
     CDS             complement(24661..25131)
                     /locus_tag="ACHGMT_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFN6834620.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(25179..26093)
                     /locus_tag="ACHGMT_000853"
     CDS             complement(25179..26093)
                     /locus_tag="ACHGMT_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFN6834621.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            26239..27042
                     /locus_tag="ACHGMT_000854"
     CDS             26239..27042
                     /locus_tag="ACHGMT_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFN6834622.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            27220..28167
                     /locus_tag="ACHGMT_000855"
     CDS             27220..28167
                     /locus_tag="ACHGMT_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFN6834623.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(28235..28528)
                     /locus_tag="ACHGMT_000856"
     CDS             complement(28235..28528)
                     /locus_tag="ACHGMT_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFN6834624.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYNPNLEEPTYHFKKQ"
     gene            complement(28556..29221)
                     /locus_tag="ACHGMT_000857"
     CDS             complement(28556..29221)
                     /locus_tag="ACHGMT_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFN6834625.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(29361..29993)
                     /locus_tag="ACHGMT_000858"
     CDS             complement(29361..29993)
                     /locus_tag="ACHGMT_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN6834626.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(30000..31067)
                     /gene="liaS"
                     /locus_tag="ACHGMT_000859"
     CDS             complement(30000..31067)
                     /gene="liaS"
                     /locus_tag="ACHGMT_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFN6834627.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(31064..31795)
                     /gene="liaF"
                     /locus_tag="ACHGMT_000860"
     CDS             complement(31064..31795)
                     /gene="liaF"
                     /locus_tag="ACHGMT_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFN6834628.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
BASE COUNT         9585 a         6477 c         5315 g        10575 t
ORIGIN      
        1 attttctgtg aacagaacta tagaattccc taaaacttgt ccattatttc aaaatttgtt
       61 atagtctttt tattcaaagc gtaaaaggag atcctatgtt aaaagaaaaa atgaaaactc
      121 tccccatgct gccgggtgtc tatcttatga aagaccgaga tggtaaggtc atctatgttg
      181 gtaaagcaaa acggctcaaa aatagagtaa gcagctattt tcatcagaat aaacagcatt
      241 caaaaaaagt attacgtatg attcatcata taacagattt tgatttcgtc gtagtagaca
      301 cggaattaga tgctttattg ctcgagtgtc aattgatcca acattaccgt cctttttata
      361 accgacaaat gaattatttc agtaattata attatgtaca tattaccaat aagggatttg
      421 tattaacaga tactcctacg gctcgaacat acgggccttt tcgtctctat aaaaaaatgc
      481 cgtctatttt gcgtataatg gaagaaactt atcaaatgcc ttggttgtct gaaattagtt
      541 tgttagcttt gcgagtccag cttcctgacc tacaggaaat gacttttgaa cagaaaaaaa
      601 aggaactcca agggctgttt caaggccgaa ataaaaagct actgacttac ttgaaaaaaa
      661 gacaacaaca tttcatctat cagttgaatt tcgaaaaagc cggtatgctt caaaaagata
      721 tcgaattagt tacttatttt attagacgga tccaggaaca aaagcagttt ttaagaacac
      781 catcgctcac cttcagcatg ccattagcag cagatgagag tcagaaaaag cactacctcg
      841 tttgttatgg tcaacttgca gaaacaatga ttacctctgg tgacgtttct cctgacttct
      901 attatgaaaa gaaagaagct catttatctt tgaaacgtca attgtcaaaa gaagaaatcg
      961 atcctgtaca aatcttgatc agttaccgca aaaaattaga aaaagaacag tttgaaattg
     1021 aactgttaaa caaaaaagaa gctgaaaagc aactaaatta gttgcttctc ggcttctttt
     1081 aggcatcttc aacttcaaat gtcaattatc ctaaaattta ttggattaag tcatagattt
     1141 ccatcgctac gatatcgata ttatcaaatt cgtattgctg atgagtatct gtttcagtta
     1201 attcaaatgt atcggtcgct ttatcataag ttactgtaca cttctctacg ccttcttttt
     1261 caaaacggcg aacttgcagt tcattatctg ctgactcgac catagcctcc agtcgtttta
     1321 tgattgcgac taattgtgaa tgtgtcatcg gaggtcctcc ttttcttatt tctgttatat
     1381 tgtatcaaaa gcatagccta cttgcattga ttttctctaa aaaaactcgt tttttatgat
     1441 tttttttctg gtatttttcc ttcaggccac caaagtttga tcaaagcttg ggttccgtca
     1501 ttctcaaaac ctttatctgc caactcttca tatagtttca atgcttgggt agttgctggc
     1561 aatacaagat ccattttttc tgcttcttct aatgcaatct ttaaatcttt gataaaatgt
     1621 ttgacaaaaa aaccaggtgt gtaatcttct tttaaaatac gaggagaata attgcttaaa
     1681 gaccagttag ctgcacttcc acctgaaagc gtctcaatca ctttctttaa atcaagtccg
     1741 tttttttgtg catagacaag catttccgtc attcctgtca tcgtacctgc aatcatgatc
     1801 tgattagcca tttttgtatg ctgacctttc ccagcagaac catgcaatgt aaatgtcgta
     1861 ccaaattctt tgaataaagg cagtacttga tcataaacgg cttcttctcc accgaccatg
     1921 atcgtcagtg taccattctt tgctcctaag tcaccaccgg ataccggtgc atctaacgca
     1981 tctgcgcctt tttcttttgc tgtttttgca attttttctg ccaggcttgg tgtacttgta
     2041 gtcaaatcaa ctaaaatatg tcctgagata tctgcagaaa aaatccccga ctgtccatag
     2101 tagatttctt ctacatcttt ggggtaacct accattgtaa agagaatatt actttgttct
     2161 gctaccgctt ttggtgtatc tgcccagata gctccttctg ttactaaagg atcagctttt
     2221 gcttttgtcc gattatagac agctacttcg tgtcctgctt ctagtagatg gcgtgcgaca
     2281 gcacttccca tgacgcctgt tccgataaaa ccaagtttca ttttttcgac ctcctaaaaa
     2341 tcaaaaatcc ttgcttctat gctagtatac gctagaaaca aggatttttt cataaaaaat
     2401 gtttgaattt tatcttttaa atgaatttaa ttttattttg attcgttcaa tcgagtcttc
     2461 ttcttctttt cttcgcatct cttcttcatg ataaacaacc atgtcttgat aaaattcgat
     2521 catagcttcg ccgaaatgtg tagatgaaat atcataaagt ttagcttcta aaacttttgg
     2581 atctttagga acttcactgg aaatataatc aataagtgct ggtgcaaagt cttcgtcggt
     2641 ttcaaaagtt ttccctaatg aaggatgatc gaacaatcgg ttcaaataag catttccttc
     2701 cacgacacaa ggtgttccgg ctgccatcgc ttctgtataa gtaagccctt gtgtttctga
     2761 agtagaagca ctgacaaaat aatctgccgc tttataataa agtgctacat cattgttgtc
     2821 cacttctcct gtgaactgca caaattcttc taactgcagt tctgagatca attcctctaa
     2881 ctcttctaca taaggtcctt ttcctacgat aactaatctg gcgttaggat aagcggagat
     2941 gacctgagga aacccattaa tcaaggcttg gatatttttc tcataagaaa tcctgcttaa
     3001 agaaagaatc atcaaatggt tttcctgaag acctagtgac tcccttaatt tgctgatatc
     3061 ctgctgagtg atatcagaac gtttgaattt ttcaatgtcg ataccagtag gaatcacgcg
     3121 aagcggtgct attacaccat agctgcgtaa tgtatcaata acacgttcac ttggacaaac
     3181 tacacctgtc gtatgatttg caaataatct agacaagtat tttacatggg attgcctgat
     3241 cacttttcct ttaccgatat aatgaaggta gtcttcatac atcgtatggt aagtatggat
     3301 cagaggtatt ttcaacttct tagctaccat cttcccaagt atccctgctc cgaattctgt
     3361 atgggtatga ataagatcta gttctagttc tttggcaacc aaataagcat accacatgcc
     3421 tctaacaaca actcttcgat ccttgaaaga aataaatggt acacttggca ttcgaatcac
     3481 atctttttcc aacttatccg cattaggatc ggtcgttgta aaaatataga cttcatgccc
     3541 atgtctttct aattcttgtt ttaatgtttt gatcgatgta gcaactccgc tgacttgcgg
     3601 aaagtaggta tcggtaaaaa agccgatttt catgtttttc gcctccaaac tagtccttga
     3661 cctgatttac tttttttaat ttgctagaga ctttttcatt cagtacactt tcgtatacat
     3721 ttttaagttc ttcaccgatt ttattgatac ttcgctcttt tgctgtttga taaccggctt
     3781 gtgtcgtact cggtagttct tgattggtaa tctgttggat cagtcggata aattcttcgt
     3841 ttgtcttccc catatagcag ttcttgctat cttctaacca gccattatac actggaatat
     3901 ctcgaacaag tacgttttgt ttgcttgcta aagcttctaa aacaacgatc ccttctgtct
     3961 cttcataaga agggaaaaag aatagatctg cacctgaata cgcaccttct atgatctctc
     4021 cacgaatgta accaggaaac aggacatttt caggatgatc ttcttttaca attttccgga
     4081 tgttccgagg aatcgagtac atgggaacat gaccaaacca aataaatgtg tattcgggca
     4141 tcttcttagc aatttctacg aaatcaatga ttcctttacg ttcgaaaaac aaaccgacac
     4201 aaatgattac tttttgatcc ggagacagtt tgaaatattc tctgaacttt tgttctttca
     4261 ttggatcagg gtgatatttc tctaagtcga tcccattgga aatagcttga atcggcacac
     4321 ggataccata gctttccaac aactgttttg aataaggtgt tggcgtgatc agatagtccg
     4381 ctttttggta caatcctacc agatattttt ttacgatagg agaaagctgg ttcgatctga
     4441 taaaggaatt tcggaaatct tcctctgtag aatgcgcatg atagacaact ttttttcctt
     4501 ctcttctggc tttattgacc atattatgac tattgattcc gtatgtattg atgtgcagga
     4561 tgtcataatc ttctttttca tctagtgtat aagagatacc gacttccgtc agagcacgtt
     4621 tttgatgatc aagcgcacgc ccgattccag attttgcaag catcttttca ctttcaaaat
     4681 ataataatac cttcacctta cgtccccctt cacttatata gtataacata gcctacaaac
     4741 tgtcactcaa acttaagccc tatttttgtc tattcttact gacaaaaacg ttttcttaaa
     4801 aaagaacaaa aaaactggcg tagctctttt acaagccaca ccagcttttg atacgttgtt
     4861 tattttacat attcatgaac aagttcaacg acttcttcca tcatatcgca ttctttcaat
     4921 gcacgatcag caagttcagc cattttcgct gtgtccaagc gtttcattaa actacgcgtt
     4981 ttaagaatag atgtcgcact cattgagaac tcatctagac ccatacctac tagcaatgga
     5041 acagctgttt gatctccagc catttctcca cacatacctg cccattttcc ttcagcgtgt
     5101 gctgcatcaa ttacattttt gatcaaacgt aaaattgatg ggttatatgg ttggtacaag
     5161 taagaaacgc gttcgttcat acggtctgct gccattgtgt attgaatcaa atcgttcgta
     5221 cctacagaga agaaatctac ttcttttgca aacttgtctg ctaagactgc tgctgctgga
     5281 atctcgatca tgatgccgac ttggatcgta tcagatactt ccttgccttc acttatcaat
     5341 ttttgttttt cttcttcaaa gatcgctttt gctgcgcgga attctttgag tgtagctacc
     5401 atagggaaca tgatacgtaa attaccatga acagatgcac gaagcaatgc acgcatttgt
     5461 gtacggaaca tttcgtcgcc ttgttcagat aaactgatac gcaatgcacg atatcctaag
     5521 aatggattca tttcatgagg taattgcaga tatggtagtt ctttatctcc accgatgtcc
     5581 attgtacgaa caactacagg ttttccttcc ataccttcta atacagcttt gtaagcttca
     5641 tattggtcat cttctgttgg gaagtctgga gaatccatat acaggaattc agtacggtac
     5701 agaccaactg cttctccacc attattatgg acaccaacta gatcttttgg tgttccgatg
     5761 tttgcagcta attcaaaatg cttgccgtca gctgtgacag tttctgcatt ttttaatttt
     5821 tcccattctg ctttttgtgc tgcgtaatct gcacctgctt tttcaaattc agctttttgt
     5881 tcatctgtag gatcgatgat tacatcgcct tcgatcccgt tgacagccaa aatatcgcct
     5941 tctttgactt tagcagtaat ttcttttgtc ccaacgattg ctggaatttc taaagaacgt
     6001 gccatgatag cagaatgtga agtacgtcca ccaatgtctg ttacaaacgc ttttacaaaa
     6061 ttgcgatcta attgtgctgt atcacttgga gtcaagtcat gtgcaacaac gaccacttct
     6121 tcgttgatca ttgatggatt tggtaaagta acgcctaata agtgcgctaa aatacgttta
     6181 gcaacgtcac gaatatctgc agcacgctct tgcatataag cattatcttc cattgcttcg
     6241 aacataccga tatacatatc cgttacttct tttagcgctg attcagcatt tacgctgttg
     6301 tctttaatat tttgcttgat ttgacctacc atttctggat ctgaaagaac catcaaatgg
     6361 gcatcaaata cttgcgcttc tgcttcacct aagctttgcg ctgctttttc acgaatttgc
     6421 tgcaattctt cagtagattt agctaatgca gcatccaaac gagcttcttc agccgcagta
     6481 tcttctactg ttgccttact gaatgttaaa tccggttgaa ctagcaggta agcttttgca
     6541 acggctacac cgtcactagc ggcaatccct tttagcattt caaccattat tcagataagc
     6601 cttctttctt cattgtttca acgattgctg ccattgcatc agcttcgtca gcaccttctg
     6661 cagtaatagt aacatcagaa ccttgaccta cacctaaaga cataacgccc atgattgatt
     6721 tcaagtttac tgatttacct ttatactcta agtttacatc agagttaaat ttgcttgctg
     6781 tttgtactaa tagtgtagct ggacgagcgt ggatcccagt ttctgctact acgtgaaatt
     6841 ctttcttttc catattaatc ggtctccttt aagtgagtgt tgtttttatg tgagggtttt
     6901 cttttttagt aattcagaga atcctaacaa atgccaccct ttcatctgta aagattaaca
     6961 ttttttttcg ttagatacaa cagttttcat tcaatttcag ataaaagtat tcaaattatt
     7021 ccggattctt tttcatcctt ttcttcacat tttataacaa acgtgtttat ttcctttttc
     7081 ttgtcgcttt ctcttctcct gacgtttatc attctttcct ttgaccctct ttgttttcat
     7141 atgaaacgaa ttacttgctt ccctttttat ttttagtata ataagagtca aaggtcagga
     7201 atggtcaata gagatttcac cttttctacc tcataaatct cttaaaaaac taggggttat
     7261 tcttttacct catgaaaaac gagatgtaaa atatggatgt acccattttt gtgaaaggac
     7321 gtgaatgatt catgctttgt caaaactgcg gcaagaatga ggcaacgatt cacttgtatg
     7381 ccactgtcaa tggtcagcgg acacaactcg attactgtca gagctgttat caaaaaatca
     7441 aaaatcaaca aaatggaggt ctgactaata tggcccaaaa tgatccattt ggatttggca
     7501 gtttggacga tcttttccgt tccatgtctc gccaaatgca acagcaagga aattatgagc
     7561 aaacaccacc aacacaattt ggcggaaaca acaacttcaa tggtggtcag ccacctcaag
     7621 gcaatacaga aggtttattg ggagaatacg gaatcaatat cacagaacaa gcacgacaag
     7681 gtgatatcga tcctgtgatc ggacgcgatg acgaaatcaa acgagtcatt gaaattttaa
     7741 atcgccgaac caagaacaat cctgtcttga ttggagaacc aggtgttggt aaaacagcag
     7801 ttgttgaagg tttagcacaa aaaatcgtag atggtgatgt accgcaaaaa ttaatggaca
     7861 aagaagtcat tcgtttagat gtcgtatctt tagtccaagg tactggtata cgaggacaat
     7921 ttgaagaacg tatgcaaaaa ttgattgaag agatccgtca agcagaaaat gtgatcctat
     7981 tcatagatga agttcacgaa atcgtcggtg ctggttctgc tggtgatgga aatatggatg
     8041 ctggtaatat tttaaaacca gctttagctc gaggagaatt gcaaatggtt ggtgctacta
     8101 ccttaaatga gtaccgcatc atcgaaaaag acgctgcttt agaacgtcgt atgcaacctg
     8161 tacgggtaga tgaaccaact gtcgaagaaa cgatcagtat tttgaaaggt ttgcaaaaac
     8221 gttatgaaga ctatcatcat gtaaaatata ctgatgaggc catcgaagca gccgcaactt
     8281 tatccaatcg ttacgtccaa gatcgcttct tgccggataa agccatcgat ttgctagatg
     8341 aaacaggttc taagaaaaac ttgacgatcc aaatcgttga tcctaaaaca atcgagaaaa
     8401 aactgcaaga agcagaagaa caaaaagttt tagcttctcg agaagaagat ttcgaaaaag
     8461 cagcttatta tcgcgatcag atcaataaac ttcaaaaaat gaaagaacgt cagctcactg
     8521 aagaggaaac acctgtgatt accgaaaaag atatggaaaa aatcgttgaa caacggacag
     8581 gtattccagt gggtgaatta aaagaaaaag aacagacaca actgaaaaac ttagcagatg
     8641 acttgaaagc tcatgtcatc ggacaagata acgctgtaga tcgagtagcg aaagcgattc
     8701 gccgaaaccg tgttggatta aacaaacaga atcgtccaat cggctccttc ttatttgttg
     8761 gaccaactgg tgtaggtaaa acagagcttg cgaaacaact tgcctacgaa ctattcggtt
     8821 cacaagattc aatgattcgt ttcgatatgt ctgaatatat ggaaaaacat agtgtctcta
     8881 aattgattgg ttctcctcca ggttatgtcg gatacgaaga agctggtcaa ttaacagaaa
     8941 aagtacggag aaatccttac agtcttgtct tgttagatga agtagaaaaa gctcatccag
     9001 atgttttgca tatgttcttg caaatcttag atgatggtcg attgacagat gcacaaggaa
     9061 gaacggtcag ctttaaagat acgatcatca ttatgacaag taatgccgga actggtaaag
     9121 tagaagcaaa cgttggtttt ggtgcggcac gcgaaggtgt tactcgttct gtattgaacc
     9181 aattaaacaa ctactttaca ccagaatttc tgaaccgttt tgacgggatc atcgaattca
     9241 gtgcattatc aaaagaaaac ttgatgacaa tcgtcacact aatgctagac gatgtcaacc
     9301 aaatgctggc agcacagcaa cttcacatcg aagttcctac gaatgtcaaa gaaaaattag
     9361 ttgacttagg ctatgatcca tctatgggtg ctcgtcctct tcgacgcacg atccaagaac
     9421 aaatcgaaga tggtatcgct gaattttatc ttgatcaccc ttctatccat gagttgaaag
     9481 caaaactgga taaagatgga aaaatcatcg ttacttctaa accagaacgt ttggctaaag
     9541 aatcagccga agaaacggct gaataaatcc agttttatag cgtgagacgg tatttctttg
     9601 agaaatgccg tctcattctt ttttcagaaa agtcaaaata ctgttcattt tataaaaaag
     9661 aaattataat aagagaaaca agcaattaaa aatacataaa agaggtgagc gccatgaaat
     9721 tggtaaatgt aacaaacagc cattcccgtc ttgtattaaa ccagcttgaa aacactgatg
     9781 cccatctagt caaggtctac acagccggaa ataccactat tgtctacact gaagcccctg
     9841 aacataatga aattctgctg atcaatgaca aacgtaaaat ccagccgaaa gaaatcgaag
     9901 atgctaaaaa ttatttctta gataaaatca aagacgccgt ttaccacgag gatgaaatca
     9961 aagtaatcga attggcaggc cttgttgaaa tctcgattcc aaaacatgaa ttaggcgcta
    10021 cattagccgt gtaaacaaat tttgaacata ataattaaag agcgtgggac aaaagtaaag
    10081 aacacttttg tctcacgctc taaacacgta taaacggcga aagcagaagc aactccttcg
    10141 gaaataagct gaaattcacg aaaattgctt ttcaaatttt tgtgaattcc agcttatttc
    10201 ttggagttaa acgcttctgt ctcagcctct ttttctttct agctttttga tttttctctt
    10261 ttcttttgta cttttctctt ttcattttca gttctacgcg tgtctttatt tttaactttt
    10321 tcctctttat ggatgattga tcgtactttt tgtgcaattt gctcgttttt gtcccagtct
    10381 tttacatcta ataatcgaca taatcgtaac atctcttctt ttttctgttt ttttctttca
    10441 cgtctttgcc attctgtttc catgtgtcac actctccttt ctttaatcat atgtacgcaa
    10501 aataattttt atattttttt gattagacat ttctatgatt ttaatatagt aaaaagagcc
    10561 gtcacggaaa atccgtcacg gctctaaaca aaatatgaga ggctgttttt gtttggctat
    10621 tcttattact tgatgcaggg cagcttgtgt caagacttct ttacatcaaa ctttttaatt
    10681 ttacattggg atatttatcc gcaaaccagc gttctgcaaa ttggttctcg aataagaaca
    10741 acggttggtc gaaacggtca cgagccaaga tattgcgact tgaactcatc ttctcatcca
    10801 aatcttctgg atcgatccag cgagcgattt tgtgtcccat tggagacatg attacttcgg
    10861 cgttatactc atttaacata cgatattgga atacttcgaa ttgtagctga ccaacagctc
    10921 caatgatata ttcttccgtt agatatgttt tataaagctg aatcgctcct tcttgaacta
    10981 attgatagat tcctttgtgg aaggattttt gtttcatgac attttttgca gtcactttca
    11041 taaacagctc tggagtaaat gaagggagtt cttcataagc aacttttagt tttccttcga
    11101 acaatgtatc cccaatctga taattgcctg tatcatatac accgatgata tcgcctgcta
    11161 ctgctttttc gacgttttct ctcgaatctg ccataaattg tgtgacattg cttaatttca
    11221 gttttttatt tgtacgttcc aaccagacat ccattcctcg ttcgaatgtt ccagaacaga
    11281 ttcggacaaa agcgattcgg tcacgatggg ctggattcat atttgcttgg atcttgaata
    11341 cgaaacctga aaattcttcc tcataaggac ttaccatctg atcatcttgt gttttgtgtc
    11401 cataaggagc tggtgccaat tcaacgaaag tctctaaaaa agtctgtaca ccgaagttag
    11461 tcaatgcaga accaaaaaat actggtgtct gatcgccacg agcaattttt tcttgattaa
    11521 atgcatctcc agcctctttg attagttcta cttcttctaa gacttgttca tataaggaat
    11581 tttcagttag aggatgattt tcgggaagca gtccatcttc atccaattct actaatcgtt
    11641 catcctgata attttccgga cgataaaact ctacacgatt atggtagata tcataaagac
    11701 cttctagccc tttacccata ccgattggcc aattcatcgg ataagattcg atatctaata
    11761 gctcttctaa ttcttctaac aattccaatg gttcacgacc atcacgatct agtttgttga
    11821 taaaagtaaa tatcgggatg ccacgttttt ttacaacttg gaataatttc tttgtctgtg
    11881 cttcgatccc ttttgcacta tcgattacca tgaccgcact atccacggcc atcaaagtac
    11941 gataagtatc ttcagaaaaa tcttcatgtc ctggtgtatc caagatattt actcgtttcc
    12001 cttgataatc aaactgcata acagaactag taacagaaat cccacgttgt ttctcgattt
    12061 ccatccagtc tgatttggca aaattacctg tttttttgcc ttttacggtc ccggcttgac
    12121 ggatagcccc accaaacaat agtaattgtt ctgtaatcgt tgttttccca gcatccggat
    12181 gggaaatgat cgcaaaagtt ctgcgattgt ccacttgttg ctttaatttt ggattattca
    12241 taacctaact ccatttcttc cattcaatta tttatatttt ctctaggcac agattgtccg
    12301 atctcttaaa gaaagacaat ttcatatagg gctgtgacaa aagccgtgta gcagtctctt
    12361 tttccgagta aacggcgttc tagagacagc ctatcagcaa taagcccacc caagccaaaa
    12421 atacgaaaag ctgtttttgc ttggctgctc ttattactta atgcaggata gctttttcac
    12481 aacctttgtc aactactaca actgaatcag tatagcatgg attcagcgaa tgaaaaagcc
    12541 accaaaccat aagagtcaaa tatttttttg tcagctgact aaaagaaaag acgtgttgct
    12601 tgtttattca agcaacacgt ctttcataac caagtttcca attatcttct tggctcgtca
    12661 tcttcgaatt cttttctgaa gtatcttcgt tcttcttcag gttccgcttc tgtttcttcc
    12721 ggatcatgaa aaatgacacg gattttaagg attcgagaac cttccatttc ttcagaaacc
    12781 agcgtaatat tatcgacatc aaacgataac ttttcgcctt catccggaat catgcctaat
    12841 gctgtaatca aataaccagc catcgtatct acatcactca tatgcagatc actgtcaaaa
    12901 gcttcattga actcatcaat cagcatacgt ccttggatga tatattcgtg ttcatcgatt
    12961 ttttcataaa gattctcaac ttcatctgat tcatcatcga tttctccgac gatttcttct
    13021 aaaaggtctt ctagtgttgc taacccaacg acgccgccat attcatctaa taaaatagcc
    13081 atctgattct gcgtcttttt catttcatat aacagatcat caataaagac cgtttcagga
    13141 acgaataaag gctcctgcat gattttcttg atatctaaat tatcaaagcc gaatttatgg
    13201 gcagctttta gtaaattctt tgtatgaagg attccgacta ctttatcttt gtcttcgtta
    13261 taaacaggga ttcttgaata gttttcggat aaaacttcat tcacattttc ttccacagtg
    13321 tcgttgatat caatcataaa tgcatccgtt cgtgggacca tgacttctcg ggcaactttc
    13381 gtatcaagag aaaaaacccc ttggagcatt tctaattctt cattattcaa tacgccttcg
    13441 ttttcaagca tataacgcat ttcatctctg gtcatttttg aatcttcatc atcaaaagtc
    13501 atcggcgtga tacgcgccaa taaacttgtg gaagcagaaa gcagccagac gaagggctta
    13561 gctactacac caatcaagcg tatcagccct gaagtgaact ttgcgacttc ttctgattta
    13621 tttaaagcaa tacgtttagg gtacaattct ccaaaaacaa tcgatacata ggtcaaaata
    13681 gccagcacaa tgatatttgc aatatttttt gccgctgtgc cgccacctaa taccggagct
    13741 aaccgttccg ataaagtatt agctagtgaa gcaccagaca aaatgttgac caaagtgatt
    13801 cctacttgga ttgtcgatag gaaattattt ggattctgca atatttttaa aagcttttga
    13861 gatttcacat ctccttcatc agctttttgc tcgattcgat tcttattgat cgaaacaaca
    13921 gcaatttcgg aagctgctaa aaaagcattg atcaatgtca aaacgatcaa taataaaatc
    13981 tgcgctaaca gcgactgact ctcagggtca gcattcataa tcgcattctc tccttataaa
    14041 attaattttt agtttacgat agaaaaaaac gtggcactat agccacgtta aaatggtatc
    14101 acaaagcaat tttctttgca ataaaaacaa gtgtactaaa actacctacg aggtcgtagc
    14161 aattttcttc ttgttttcat tttgcttgtc ttctttgacg attttgatta tataggaaat
    14221 gatcgtcttg cagattgcgt aaaaaggaac acctaagaag ataccaagca atccggcaat
    14281 atttccagct actagaagaa cgataatgat cgttaatgga tggattttca gtgatttccc
    14341 aattacatta ggataaatga tattcccatc caactgttgg acgattagca caaccacaca
    14401 acaaagtaat gcttttaccg gatcattgaa gactgttact agaaatgcag gtgctagtcc
    14461 tagataaggt cctaaatatg gaattaggtt tgtcaatccc gcaatgaccc caaataagaa
    14521 agcataatcc acaccaatta ccatatatcc taaaaatgtg aaggtaccaa cgaataaaca
    14581 ttcgattgct tgtccgctaa tgtaattagc caatgtttta ttcagttgcc ccaataactc
    14641 cacaatttgg tttcttcttt tttctgggaa aaactgttgg atgtttggca ctagtttttc
    14701 tccgtctttc aacatataga acaagatgaa tggtgctgta atgattacta cggttgccga
    14761 agcgatcgtt ccaacgatcg aacctaaact attggatact ccactaagga attgctgaat
    14821 gattgtcccg taagaaatgt ctaattgctc aaaatattta ttgatatcta cttccttgaa
    14881 caaaggcaat tcggctacct cttttaacca gctttctacc tgtttgatga aatcaggcat
    14941 attagatgcc aatgaggaaa tttgctgtac tagacttggg atgacactaa gtaaaatcca
    15001 aataattgct gcaactaaaa ggagcagcac aattgcgaca gcccatattc ttttgatttt
    15061 tgctttcatc aacagcttga caataggatt caataaatag taaagaaagc ctgcaatcag
    15121 tacaggtgca aataacgtgg aaaaaaatgt cccgatcggt tcgaaaataa agtttatttc
    15181 tgatgaaatc atgatcaatg cagctatgat caataattca agcgaccaaa acattagttt
    15241 agactgtcta agtttttcaa acaatagatt tcctcctttc tttcttacta gtatatatta
    15301 tattcgttaa aaattctttt attctttttt tatttttgaa aacttgccat actttttatt
    15361 tgttttattt tccagtatac tatacgaaag gaaggtggct agaccatgaa agttgtacaa
    15421 acaaaagata caatgagcaa tatttactta gatgctgtac ggatcaggca tcaagttttt
    15481 gtagtcgaac agggtgtgcc tttgtctcga gaaatagaca aagatgaagc ccattgtatc
    15541 cacttcgtcc tgtactcaga taaaaaagaa ccgcaaggaa ctgtacgtct tttgccttta
    15601 gaaaacggaa aaatgaaatt acagcggatg gcgattcttt ctgaatacag acaccaaggg
    15661 ttaggaaaaa tattgattga agaagcagaa aactttgcta agaatcaagg ctataatacg
    15721 attttattag gggcacagtc cacagctgaa actttttacg aaaaactagg atatacagct
    15781 tacggcgatc cttttgaaga tgctggcatg cctcatattt atatgaaaaa gactttataa
    15841 aaatttattt cgaaaaaagc aaaaaacagc aagcttttga gaaaaatctc tcactggctt
    15901 actgttcttt tttgtgttta cttacgcgac aattgcttgt aacggttata ccaaatatca
    15961 atatagcctt ccgagaacgg gccatctcca ttattgatcc agtcaaccag aatcttcaca
    16021 ttttctttta ggatgaagtc aatatcgttc gggtaattca tcattttgct gtgcatctca
    16081 tattcatcga catccaatag tcgtttctcg ccgtcaggaa ataccttgat atccaagtca
    16141 tagtcgatat atttcaaggc ctcatcgtcc agcaaaaaag gtgaagctag attacaataa
    16201 taggaaaccc ccttttctct gatcattgct attacattga accagtattt tttatgaaaa
    16261 taaacaatcg ctggttcgcg cgttacccat cggcgtccat cagattcagt gactaatgtg
    16321 tgatcattca cgccgatcat agagtgttcg cttgttttta ataccatggt atctcgccac
    16381 gttcgatgca atttgccgtc gtgcttgtaa ctttggatcg tcacaaactc tccttctttt
    16441 ggaactccca tagcaaccct tccttaagca gcattttgtg ctttgtgttt gggtgttcat
    16501 tacaccgaca tcataattat tatatcaata aaaatgagct ttgcctagaa aggattccta
    16561 ttgtttcgat ttttctctaa ctgatctacc agtttcattt ggaccttagg aaaaacgacc
    16621 gaatcaaatg catcaggctt caaccacttc gaagttttgt tctcggtaaa ttcttgttct
    16681 gctccctcgg ttgcccgtcc gtaaaaaagg agaacatgcc atttcagatg actaaaaata
    16741 tgagtaactt ctcctaaatg tcgtgtctgc caaacaaaga gttcctgctt ttcaaatggg
    16801 agattttgct cttcttctgc tactaggtca tcaaacagat cgatttctgg ctttgtttcc
    16861 cattcttttt tcaatcgctc atactcctct tgtgtcactt ccatcattgg aaatgtccac
    16921 atattcgcca gcaatttttg gctatctctt tcttcaaaat aatacgctcc agaatgattt
    16981 tgaagagctg cactgatata ataaacgtct tttggctttg ctttttttgt ttttacagga
    17041 aaactcgttt ggattcctcg tttgttagct aaacaaaagg cttggatcgg acaagtctca
    17101 cactttggag aagttggtgt acagatagct gaacctagat ccatcattgc ttggttgaat
    17161 tcacctggat gcttttcatc aatgattttc cgcatcgctt catcaaagat tttcctgcta
    17221 gaagcttttg cgatgtctgc ttcaatgcaa aacaatctgc tgactactcg catcacgttg
    17281 ccgtcaactg caggttctgg aagtccaaat gcgatacttg caatcgcgcc tgtcgtatat
    17341 ggtcctattc ccttcaatga gctaatttct tcaggcgttt gcggcatttc cccatcaaat
    17401 tctgacatga tctgtttagc agcagcttgg atgttccggg ctcttgagta ataaccaagg
    17461 ccctcccatg cttttaaaag cttttcttct ggagcatttg ctagctcttc aattgtagga
    17521 aaccattcca tgaaacgata aaaataatcg atcactgtgt ctactctggt ttgctgaagc
    17581 atgatctcag agatccagat acgataagga tcacgattat agcgccaagg caggtttctt
    17641 ttttcttgtt cataccattg aatgaactga tcttgaaatt cttttgtttc tttatctgtc
    17701 catttttccc attctttatt cactttcctc ttccttttcc gtaatgaatc gctggatcat
    17761 atccaaatca aatccttttt gatataagct ttgctttact tttagatttc tttttttagg
    17821 ttcaaacctt aaatttcttc gccatagttt atctccttgt ttgactaaat aatcatattc
    17881 tatttcttga tctgcttctt gcggaagatc agctatcgct tgttggatca cttcttgaga
    17941 aaagcctttc gtcatcaatg tttgttggat ttttctcaat aattcttttt ggctttttcc
    18001 gtgatttttt ccaaacgctt tttcggctgt ctgatgtgca ttttgaactt ggtcttcaaa
    18061 tgtatattgg ctgagcgctt gttcaatgat ttcaggtttc aaaccttttt gctgcatctg
    18121 ctgcgctaat ttctttggtc ctttgtcact taagcgcata ttcgtgcgca cataactttc
    18181 cccatagacc aaatcatcga caaggtccaa ttcttttaag cgggctacga ttttatttcg
    18241 atcttcttga aggatttcct tttcttttaa gtatgttcgg atttcttttt ccgatcgcaa
    18301 ctgataactt aagtaattca ttgctaggtg aaaaccgaaa tcagctccgc tactcttctt
    18361 gatctcttgg atagtttctt cgtctaattc ccgtcctttt aataatcgat agcgtactaa
    18421 tacatcttca gatacttgta aagcttctcc ttcagataaa tctacgcgat agaaagggcc
    18481 tttgccttta ctgattctcg taacggttaa cataaacatc ctcctcgaac atatattctc
    18541 tatcatacca cagaaaatcc ccgtaatcag gtattatatg ttagaataaa ccacagaaaa
    18601 aagaaaaagg gagatactta tggcagaaac aattgtcaaa actggacaaa cggttccttt
    18661 gaaaatcaag cgtttaggca taaatggtga aggaatcggt tatttcaaac ggttgatcat
    18721 atttgttcca tatgcactac caaaagaaga agtactagtg aaaatcacga aagcaactcc
    18781 tcgctatgca gaaggagaat taataaaagt caaaaaaaca agcaaagatc gcgtagtggc
    18841 accatgtcct gtttactatg aatgcggtgg ctgtcaatta caacacttag cttatcaagc
    18901 tcaattggat tttaaaaagg atctgctttt gcaagcattg gaaaaattca aaccagcagg
    18961 ctttcgatct tatcaacttt tgccgacgat cgggatggat gagccttggc attatagaaa
    19021 caaagcacaa tttcagttac gaaaaaataa gcagactcaa aaaattgaag ctggtttata
    19081 cgaagcaaac tctcatcagt tagtccctat aacagactgc ttggttcaag aacctcgaac
    19141 acaaaaagta atgaatactg ttgttcaact attgaataaa tttgatgcac ccatctatga
    19201 tgaacggaca aacagtggaa tcttccgcac aatcatggtt agaatcggtg ttaaaactgg
    19261 ggagttacaa gttgttttta ttactcgcag taaaaaattc ccgcaaaaaa acgcaatgat
    19321 ccgtgagatc aatgaacgac tgcccgaagt tgtttcaatc atgcaaaatg tccaggcaca
    19381 aaaaacgtct ctcgtgatgg gggatgaaac gatccactta tggggaaaag aagccattga
    19441 agaacaaatc aatgaagtga ctttcgatct ttctcctcgt gccttctttc aactcaatcc
    19501 tcagcagacc gaattgcttt atggcgaagg aatcaaagca ttagatgtcc agcctaatga
    19561 aacgatcgtg gatgcttatt gcggagttgg gacgatcggc ttaagtgtag caaaacaagc
    19621 caaagaagta cggggaatgg ataccgttcc tcaagcaatc gctgatgcaa aaaaaaatgc
    19681 ggaaagatta ggatatcaca acactcgata tgaagtcggg acagcagagc aagttctgcc
    19741 taaatggttg aatgaaggtt ttcagcctga tggtatcatc gtagatccac cacgtacggg
    19801 tttggataac gcattgatcc aatcattatt gaaataccct ccaaaaaaac ttgtttatat
    19861 ctcctgtaat gtttctacac ttgccagaga tctggttaat ttaagtaaaa agttcaagat
    19921 tgaatattta cagtccgtcg acatgtttcc tcagactgca cgttgtgaag ttgtcgtgaa
    19981 gatgacgaga ctcgctgact aaatgaaaga ggttgtgaaa aagtctgccc tgtatcaagt
    20041 aataagaaca gccaaacaaa aatagctctt catgttttat gaatactgta cttgtgaccg
    20101 aagagttaac aggaggacac tatttattta aaaaaagggt tgtgacatag tttttgtcac
    20161 agcccttttt gattattatt ggatattcac gtctatcact tgataaaatg catttgtcgt
    20221 atcgctgata ttccatactg ctaaaatcac attatatcct tttcgatcct gcggaagtgt
    20281 gatcgtttgg ttgactgttt ttggtggaac agttgctttg tcgtctattt ttgtgatcaa
    20341 ttcaaaatca ctaaatttca aaggttgatt aggattccaa gttggtttgg taatgtagta
    20401 atcccaagta cttgtccgat gttgtgctgt taaattccaa gtaatattta atgttcctgg
    20461 attaaccatg tttttatgcc atctgttaga tgtctgttca tctaaaggct caaagccgct
    20521 gactcctgca ctagcaattt ttccatcaat gaatgtattt ttggtagctt cgatactttg
    20581 cggttcccat tgtgctcttc cgacattttg atttaagttt cctgctgctg atgttcctag
    20641 gtatgcacgg cttcctggag aagtaatata accatgcgca aaacctggaa tgctaaaacc
    20701 gaacaaccct aaactaacta acccaataaa caataacgta ctaatctttt tcatgtagaa
    20761 acctccttca tttgataacc acattgtaaa cgctttattg tattaacaca aacattatgc
    20821 ttttataatt ttatgttgaa gtaaaaaagg tgaaaaaagt aagatgcaga ttataacaga
    20881 agtagacgag aaaagtacaa atagaaaaca tgagaatcaa atcatatgat ctttttcaaa
    20941 aacatcattc atataaacaa atgttcaact ttttaataac aacaattatc aatttttttc
    21001 ttacttcaca aactaaatac aggtcttgct gtgagccttc atggtaaact aatattatag
    21061 aaaggagtta gtaaaatgaa gatgataaaa gatattcgtt tttgggtttg tgttatagga
    21121 attgtcatat taggcttttt atcagggttg cttagcggaa atcccggaaa atattactat
    21181 tctttgcaac ttccaccatt tgcaccacct agctggatat ttgggccgat gtggacctta
    21241 ctgtatatat tgatgggtat ttctctttat ttgttactta atcacaacaa caaaaaacag
    21301 cgaaacaatc ttgtcggtct ttttgtgatt cagttcattt ttaattttat atggtctgct
    21361 ctatttttta atttaagaaa tattttcatt gcagcaatcg atattacact tttagttatt
    21421 ttcttgtcag tgcttatgta tcagttgtgg ctgcatcatc gcctggcaat gtggctcatg
    21481 attccttatt atttatgggt actattcgca actctattga attattcaat ttatttttta
    21541 aactaagcgc taaaaagaga cgtcctgtaa tttaacacat agaaaaagtg ccacctaagt
    21601 tagatttttt gtctaattta ggtggcactc tttattaacc aaactatttt tttctagtac
    21661 ccacttgttg ttccatactc attattctgg tcataaccat aaccattttg ttcctgtcca
    21721 gcgtaaggat cataactttg agtatcttga cttggtgtat agtactgact tgtatcttct
    21781 gtttgctgat aataggtatt cggatcttgg tactgatcat atgtgttttc tgaataggaa
    21841 gaataatcgg tgtacccttc actattcgat ccatcaccgt ttgtcgaatc gccatcttcc
    21901 gaatcatcaa agaacatcat tgaagcattc tcatttttca aattaggtaa tgtatcgctt
    21961 gtttttatat ttaattgttt cttcagttca ttttggatcg aaagaagatc attttttcct
    22021 aaaatttgat agtagatatc attgatcatt tgtccttcgc cttgcaactg gtcttgtttg
    22081 attgtttcaa acgccggggt gtagttcgtc gctacatcaa gcatgtcatc ccaatctaag
    22141 tccgtcgtca cattgttctc aactgctttt aaaatttttc tataattgct tacgccatct
    22201 aaacttaaaa ctttattgac gattttcgtc actacttccc gctgacgacg ttgtcgaccg
    22261 atatcgcctt ctggatcttc gtgtcgcatt cgagaatagg ctaaaccttt ttctccattc
    22321 aatgtttgtt cacctactgg tacatggaca ccatccaaag taaagtcaat tttattattc
    22381 actttgatgc caccgaccgc attgatcaaa tcttccatgc catccaaatt gatcgttaca
    22441 taatgatcaa taggaatatc gagcagttgc tcaattgaat ccatggccat ttcgacacca
    22501 ccaaatgcgt aggcatgatt taatttatct accgttccat aacctacgat attcgtataa
    22561 atatctcggt ccaaactgat gatcgttgat tttttttgtt tcggattaac tgttactacc
    22621 atcatcgtat cagaacgtcc ttgttccgtt cttcctaatc caccggtatc taaacctaat
    22681 agcaaaaccg aaaaaggttc tttgtcatca atgcttactc tctcagaacg tttggaggat
    22741 attcgattag agtttttaga aattttgttt actgtttggc tcgcttctcc cataaatctg
    22801 attccgtatg ctgataatcc ggcaatcagc aaaacgaata cccctaacaa ggttaataga
    22861 actttttgcc atcgcttcat aatacacctc tgtttcacat tttctaaaat attgtctcat
    22921 ataactgttc aatcagcaaa ttcacttttt aatcataaga aaactttaaa aaacattttt
    22981 gtgtaacgat tattattttt taaaatttct caaaattctt aactatcata ccataccgtg
    23041 aaaaagatcg aaagaccgaa tacacgcagt tcgcaatcgt tttatctttt tttatgtatt
    23101 ttttccagaa aaagcgaaca acaaggtatt ttccaaataa tcgtgcagta aaatgatcgc
    23161 acataccttt cccatctttt taacagtctt tgcctaccgt tcagattatc attcgatttc
    23221 acgacaattt cttcctatga ctggtccttt tgttaaaccc atcttctatt cgttttattt
    23281 ccagctcact gcatggctct taaaatgact cgaactagtg attgcattac catcatcttt
    23341 taatggatag atatgctcct cttcaaagta aaacgatttt cttttaaagt aatagaattg
    23401 acatggtaat gagtatgtat actagtggtt tcacaaatgg tcttttattc aacatccaaa
    23461 aactgaaatt tctttaaaat ccattactaa cgaagaaaat gttccttgct tgataattag
    23521 ttaggacatg tcaagtgaac aaatgcattt acttagccaa aaaatctaaa aacttcataa
    23581 atagacaaaa agacacgact ctctaagaag tcgtgtcttt attcaacaaa ttattataat
    23641 ttagtcactt cagcagcttg aggtccgcgt gcaccttcaa caatcgtgaa ttctacttct
    23701 tgaccttctt ctaggctctt gaagccgtct ccttgaattg ctgagaaatg aacaaatacg
    23761 tcatctccgc cgtctactgt aataaaccca aatccttttt cattgttgaa ccatttaacc
    23821 gttccgtgtt ccataaatgt atatcctcca aaaaagttct tgcaagtaac cttgcaagaa
    23881 cattataccc aatttagaaa acgctagcaa gctttcgctc ttttattttc caaattattt
    23941 ttaagactga tatgacggag gaaatggtta ttttgctgct tcgaaacgtt tgtttacttc
    24001 atcccaatta ataacattcc agaatgctgc aatataatct ggtcggacat ttttatattt
    24061 caaataataa gcatgttccc aaacgtccaa acctagaatt ggtgtctttc cttccatcaa
    24121 tggagaatct tgatttgcag tagaggtaat agctaatttt ccattttcca ttacaagcca
    24181 agcccatcca gaaccaaatc gtccagcggc tgcttttttg aattcttctt taaaagaaga
    24241 aaaatcacca aaagcttcat taatcgcttc ttttatttct cctgtaggtt cgccaccagc
    24301 atttggtgcc atgatttccc agaaaaatga atggttagca tgtccgccac cattgttacg
    24361 tacagctgtc ttgatatctg ttggaatagc gtccatatca gataataatt cttctattgt
    24421 tttttcgcct aattctgggt atttctcaat tgctgaattt aaattcgtca cataagtatt
    24481 gtgatgctta tcatgatgca gatgcatcgt ttcttcgtca atataaggtt ccaatgcgtc
    24541 atatgcataa ggtagatctg gtaatgtgta agtcataaaa aaattcctcc tcaatgtttt
    24601 cttaataatg tctacattac aaagagtaac aaagagaagg aaattcttca attgttatgc
    24661 ctagtcgtct ttttcacttt gacctacttc ttcttcaaaa acctcagttt ctttaaaaat
    24721 aaacaacttg ctgaaaatat agttcgcaag gataacaata atttgagtga tgattttcgc
    24781 cacatactca ttgctgttca taacattgat caacaatacc attgccccca tgtccaatcc
    24841 gtaagaaagg atacggtaga agaagaactt accgcattca ataagtgcat gtgtaaaatt
    24901 cggtgatttt gaatggaata cccaaagttt gttcgttaca aaagcaaaca atacagaaca
    24961 aaaccacgaa atagaattgg ataaggccat acttaaatca aaatattggc gtgcgagagc
    25021 aaaactaaca aaatttacaa tcgtagccaa accaccaaaa aagagataga ctagtatctc
    25081 ccaatatcct tttgcttcta aatatccttt taactgtcga tactgcttca tgagccaacc
    25141 taccttatta aaaattctta tttatcatac tctatatttc aataactttc atctttattt
    25201 aaaaaaatta attttttttg atcctttgcc tttgtctctg cttggatcgt cacatgtgta
    25261 ataccatatt ttaattttaa taaagaaatt atacgaggat atatcttttc aatctcgctt
    25321 agtagctgat cttcaaaatc aatatgtccg tcgaaaataa ttatcttttc gctatactgc
    25381 caaatatgga catgatgaac atcctttacc ccatcgattg cttcaatttc ttgttccatt
    25441 ttttcatagt cgatagcagg tgcactttgt atcaaaatag cggcagcttt tttgatcacc
    25501 attatcgctt ctatcaatat atatatcgaa atgatcagtg taataactgg atcgatccag
    25561 tagatatcga aaaaacggat caaaattgct ccgataatca cacttaccga agacagtgta
    25621 tcacttaaca aatgcaaata actagatttt atattcaaac tttcatctgc ttcttgtcgt
    25681 aataataata ctgagaacaa attcgcgagc aatccaataa ctgctactgt tagcatcaaa
    25741 tgactattta tcttttctgg tgaagtgaac cgcttgaatg cttcaacggt taaaaacaag
    25801 gataatatga tcaatatgaa tgagttgatg aaagcactca atatttctaa acgctggtag
    25861 ccatacgtac gttttttatt tgcttctttt cgggccattt tttgtgcccc gtaagaaaaa
    25921 ataattgcca atgtatcact aaggttatgg aaagcatctg aaacaagaga caaactccct
    25981 gacataatcc ctccaataaa ttctgataaa gtgatcaccg cattcaaaac cactactcca
    26041 aatagttttt ttgatgatct ctgtggctgt gtaggcacta ttccttcctc cattttattt
    26101 atattggata gtctttagac aagaataaca tttttttgat atttcaccaa tcattcagct
    26161 gtttccttaa agcacttgta tttcacttga tacttcgcta gaattgcaag tgatgacact
    26221 actagatgag gagtacttat gacaacaaaa caattaatta tcagttcctt tatacgattc
    26281 aaagacctaa aggaggcgag aaatgtccca ttttggaaag ccattgtcta tcttttagtt
    26341 ttgagtataa ttatggcttt gccaatctct tttcaagttt ttcaagtctt aaacaacatt
    26401 caattggatg ggcaaaagat tgctactcgc attcctgatt ttacaatcca aaacgggcag
    26461 cttcaaaccg aagaaaaaga aggattcatt taccaaacaa actccattat cttcacattt
    26521 gatccagagg gaaaacgaac agaacaggac atctctactg atttgatggg gaattttgtt
    26581 agtgtcggga tgttaaaaaa caaacttatc atagctcttc ctaatacagg cacgacttct
    26641 gctttgttga acaataacca attagaactt ccctacacca atgaatcttt aaaaaactta
    26701 acaggtaagc agctccgttc ttttttaagt gaagcatcta taccactttg gataaaagtc
    26761 ctgacattta tattttctat ttatcctagt tttctgaatc tgctgatcac tttgctattt
    26821 accaacattg ctgcttttct ctatgcacgc tttcgactaa caaaagctac ttttcttgat
    26881 tgtttaaaaa cattgatcta ttcaatttca ttgcctacta taattgcaac aatcttgatg
    26941 gtctttcttc cttcttttga cactagcgcc ttcattgctt ttgcaggaat ctttattttt
    27001 gctcaagctg tcaaaggatg ggcaaaaata tcgatcaatt aagaaataaa gacaactcgt
    27061 tatttctact tcaatagacc cttttagagg ttgtgacatt tttttgtcac agcccctttt
    27121 ttatttgaaa aatatttctt tctcaaaaaa caagcaaggc gcttcaaagt tcttcttgta
    27181 tgcgctacac ttagagtaaa gaaaggcggg aaattaacta tgtcagaaag aatcatttat
    27241 gcagatcgtc catttcgtga tgaatttatc gaagaaatcc atactatagc gcctggttat
    27301 atctttaaaa cagaattaca accagaggat ctacaaaatg tagagatcag tttgggctgg
    27361 aactcagact accaagaaaa actattagcc tcttctacct tgaaatgggt ccaatctatc
    27421 tcagctggtg ttgatcagct accccttgag actttttcaa agaaacatat tttactttca
    27481 aatggtagtg gcatccacag tgaatcgatt acggagcatg tcttaggaat catccttggg
    27541 tattctcgtg gtctttttca agcacaaaag gcacagagtg acatgaaatg gttaggttca
    27601 tcggtccatt accaatctat ccaagggaaa aaaatactaa tcattggaac tggccatatt
    27661 ggaaaaatga ttgcaaaaaa atgcaaagca ttagacatga actgctatgg aatcaataca
    27721 tccggtcatc cagcagaagg aatggaaaag actttttctt taaatcaact aaaggaagtt
    27781 cttcctgaag tagatttcgt agtcaatatc ttacctctaa cggaggaaac aactgggctc
    27841 tttgatcagc atatgtttga acactttgat ccaaaatctg tattcatcaa tgttggccgt
    27901 ggtgcttctg ttaagacaca agatctggtg caggctttaa ataaccagca actttccttt
    27961 gcagcattag acgtttttga ggaagagcct cttccacaag atcatcctct ttggaaaatg
    28021 gacaatgtac tgattacttc ccacattgca ggtttgacac ctgattttca aaagaaatta
    28081 atggctattt ttttaaccaa tctgaaaagc tactttgcca cccatgagct tcaaaccaat
    28141 caagtaaaat tgtctgcagg atattaatag ctgaaaacaa tattttcaaa agaaaaaaga
    28201 gaaggtgaca aaaattgtgt caccctctct ttttttattg ttttttaaag tgataggttg
    28261 gctcctccag gttgggatta taatcaacaa gtaaatcata tcctttaaag aaccattcat
    28321 ctgcttcctc gataaaaaaa agtgtcccat caatttccac tttctttaca ggatgttctg
    28381 gctgttcaac tgacattcca atcgaaaaac catcatgaac ctctgttttg ccgtagactt
    28441 ttccaaaaaa acgtattccg taagtttctg gcagatcaat ttctgagtca aaccactttt
    28501 gtgcttttgg cgttaccgtt aatttcatcc caacaaccta ctttctcttt tctttctatt
    28561 gattcatctt atcgtctaga atatccacat cgcctgtctc tccaataacc agtagattat
    28621 cattttcacg aacatattca tctgctgcag gagaagcgat aaccgtttga tctactcttc
    28681 gtatggcgac tactgttaag tcaaaacgtt gcctgaaatt taattcagct aatgttttgt
    28741 tgaaaaattt aggattggag actcggattt ctgctaaaga atatttatct gacaactcta
    28801 ggtaatctaa aatattcttt gacactaaat tatgcgcaat ccttattccc atgtctcgct
    28861 cgggatggac gacgcgatca gctccgatct tttcaagtac tctggcatga tactcatttt
    28921 gggcttttgc taaaatattt ggtacgccca tttcctttac catcagagta actaagatac
    28981 tggcttgaat atcttctcca atagcgacaa ttacatgatc gaaatttctt atgcctaatg
    29041 atcgaagtgt catttcatcc tgtgcatttg ccacaacagc atgagtggct atattcatat
    29101 attcattgac ccgatcttca ttactatcaa tagccagtac ttcttgacca gcttcgatca
    29161 gtgttcggca gatacttccg ccaaatcgtc caagacctac aattgcaaaa ttttgtttca
    29221 ccgttttcct cctttagtat cttcttttct cagtataaca aagcctttac aaaaacaaaa
    29281 gcaagcatca ttattgtaaa aaagccaaga aaaataatag ttattttctc agcttttgat
    29341 tatcctcttt atcatcgttt ttatttcacc aaaccatgtt tgaatgcata gatagcggct
    29401 tgtgtacggt cttccacttc tagcttagcc aatatattgg aaacatgtgt ttttactgtt
    29461 ttcagtgtaa taaacaattc gtccgctatt tcctgattac tttttccttc agaaatcagc
    29521 attaaaattt cattttcccg attcgttagt tcttcatgta atacaggctc atttcgccga
    29581 ctcatttttt ccatcatttt tgttgtcact tctggttcta atactcgctc gccacgttgc
    29641 gttgccctta tagcatcagc aatttcatgt gcggtagagg tcttcaacaa ataccctgct
    29701 gcacctgctt caatggcagg ataaactttt tcgtcatcaa taaaactggt tacgataatg
    29761 attttagctt ctggccaatc ttttaagatt gcttttgttg attcgatacc atccatttct
    29821 tccatgacta agtccattaa aatgacgtcc ggtcgaagtg tcatcgcctt ctcatagcct
    29881 tgtcgtccat tttcagcttc tccaattact tcaatgtctt cttgaataga taaataaaaa
    29941 gagacgccta agcgcaccat ttcatggtca tctactaata aaacttttat catccttatc
    30001 tatgcctcct tcatcaaagg gaccttgatt tctacacttg taccttgacc tttaaaacta
    30061 atgattttta ctgttccacc aataccagct actcgttctt tgatattgtt caatccataa
    30121 cttcccgttt tcgtttcatt catattaaac cctgttccgt cgtcaataat acgtaacaaa
    30181 agattattgt ctattttgtg caaatatacc tctaattcat tggctttagc atgtcttaat
    30241 gtatttgata acaattcttg aacgattcgg aacaagtgat cctcaatgga actagatagt
    30301 tttacatctt ctacatccca tttcagcgaa atctggattt tattttgcaa ttccttcaat
    30361 aactgctcga taccttgctt taaacttttc tcttctaaat tgactggacg tagatgaaga
    30421 agcagcgcac gcatctcaga ctgggaagcg ttgatgatct ctgctaccat tttcaactgc
    30481 ttgcgaaaca tttctggtgt ctcgcttttt tctgcttgct ccgtcaatgc cgacatcatc
    30541 atcattgctg caaacaattg ttgcgaaact gaatcatgca gctcacgggc taatcgatgt
    30601 ctttctaatt ctaaaatttc ttctttggtt tgtccatcta catattgggg acggctcgtt
    30661 acaatttgta attcactaga tacttcaatc attttttctt ttatcttagt gatttcctta
    30721 tcaatatctt tgatgtataa atcgtccgat gcacttggga tcggaagaag caacaatttg
    30781 gattcataat tgccagaaga aagtgcacgc agcttttctt cgattttccc gtattttgtc
    30841 ttttgaataa gtgataataa tagaaagaca attaatccta cacctaagga aatggctaac
    30901 aaatggaaga taagcggaac atataataaa cgtgcacgca gtaattctcc ccaccaatga
    30961 ctttgattgc tggcataaaa ataagtgaaa agtgaaaata gaataataag gaaagccgca
    31021 atccccgaat aaacagctag catcgctctg gatatttttc ccatcatatt cgtatcacct
    31081 cgacatctcc aatcaaggta ttagtaataa tcttcaatct acgagggttt tcatcataat
    31141 cactgctgta gattttgatt tgttcatttt tcaaagaaaa ttgctcttct tcaaacaaga
    31201 catttcctac taatgtcgca tgctctaatc ggatggctac gcccaaaggt actaagatcc
    31261 gtgttctgcc tatccctttt cttacaatca caatattatc atcttttggc aaaagcgtat
    31321 tgcccaaatc tatgatagta tcaccagaaa tgacggcgat gttgatatcg tcccattcat
    31381 agacgtcgtt tccaatacgt tggttaccga ataattgctg tcttttccgc tcattattat
    31441 gtgtttttaa ttgctctgtt tggatcatca tgatctgttt ctttcgccaa aaagcatttt
    31501 tggttaaatc tatccctgaa atctctacac ctttcagacc aataaataaa acggcaaata
    31561 cagccatcat ccataaagca ggactgttga ctaagctaaa aaaaatgacc aaacttccta
    31621 atatcaattg aaaattttga aatttagtac gaggatgttt gcgcatagct agatatatat
    31681 tgaaaatacc gaatatcact aaaagcaaaa gtccagtatt gtttacgatt tgccaaacag
    31741 caaataacag cagtagtgct tcaactacca caaaaaaacg ccaagaacta ttcatcgctc
    31801 tttctccctc ctgacacatt ctcatttcat catacctatt tttttaactg ttggctatag
    31861 gactttagat gtaagatcgt ttgttgaaat ctaaaataca caaagagaaa aagatctaga
    31921 gtgtatcatc agtaagatga actctctaga tc
//
LOCUS       DAZBSA010000020        31030 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000020 DAZBSA010000000
VERSION     DAZBSA010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.020"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            58..948
                     /locus_tag="ACHGMT_000861"
     CDS             58..948
                     /locus_tag="ACHGMT_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFN6834629.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(1021..1845)
                     /gene="nadE"
                     /locus_tag="ACHGMT_000862"
     CDS             complement(1021..1845)
                     /gene="nadE"
                     /locus_tag="ACHGMT_000862"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFN6834630.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1857..3329)
                     /locus_tag="ACHGMT_000863"
     CDS             complement(1857..3329)
                     /locus_tag="ACHGMT_000863"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFN6834631.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3966..4328)
                     /locus_tag="ACHGMT_000864"
     CDS             complement(3966..4328)
                     /locus_tag="ACHGMT_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFN6834632.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            4551..5303
                     /locus_tag="ACHGMT_000865"
     CDS             4551..5303
                     /locus_tag="ACHGMT_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFN6834633.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(5443..6366)
                     /locus_tag="ACHGMT_000866"
     CDS             complement(5443..6366)
                     /locus_tag="ACHGMT_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN6834634.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(6381..6653)
                     /locus_tag="ACHGMT_000867"
     CDS             complement(6381..6653)
                     /locus_tag="ACHGMT_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834635.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            complement(6997..8184)
                     /locus_tag="ACHGMT_000868"
     CDS             complement(6997..8184)
                     /locus_tag="ACHGMT_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFN6834636.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            complement(8291..8503)
                     /locus_tag="ACHGMT_000869"
     CDS             complement(8291..8503)
                     /locus_tag="ACHGMT_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein"
                     /protein_id="HFN6834637.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITMLKNDIN"
     gene            complement(8594..9046)
                     /locus_tag="ACHGMT_000870"
     CDS             complement(8594..9046)
                     /locus_tag="ACHGMT_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFN6834638.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(9059..9469)
                     /locus_tag="ACHGMT_000871"
     CDS             complement(9059..9469)
                     /locus_tag="ACHGMT_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN6834639.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            9744..9956
                     /locus_tag="ACHGMT_000872"
     CDS             9744..9956
                     /locus_tag="ACHGMT_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFN6834640.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            9959..10171
                     /locus_tag="ACHGMT_000873"
     CDS             9959..10171
                     /locus_tag="ACHGMT_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834641.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            10311..10466
                     /locus_tag="ACHGMT_000874"
     CDS             10311..10466
                     /locus_tag="ACHGMT_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834642.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(10463..10687)
                     /locus_tag="ACHGMT_000875"
     CDS             complement(10463..10687)
                     /locus_tag="ACHGMT_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFN6834643.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            10765..10953
                     /locus_tag="ACHGMT_000876"
     CDS             10765..10953
                     /locus_tag="ACHGMT_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834644.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            11092..11220
                     /locus_tag="ACHGMT_000877"
     CDS             11092..11220
                     /locus_tag="ACHGMT_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834645.1"
                     /translation="MAREEALKIGKVIADNRWGNSRPIILSKQHIEKQKAWQQIKK"
     gene            11279..11614
                     /locus_tag="ACHGMT_000878"
     CDS             11279..11614
                     /locus_tag="ACHGMT_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834646.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            11847..12788
                     /locus_tag="ACHGMT_000879"
     CDS             11847..12788
                     /locus_tag="ACHGMT_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFN6834647.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            12790..13680
                     /locus_tag="ACHGMT_000880"
     CDS             12790..13680
                     /locus_tag="ACHGMT_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFN6834648.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            13723..14523
                     /locus_tag="ACHGMT_000881"
     CDS             13723..14523
                     /locus_tag="ACHGMT_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFN6834649.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            14538..15389
                     /locus_tag="ACHGMT_000882"
     CDS             14538..15389
                     /locus_tag="ACHGMT_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN6834650.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            15590..16051
                     /locus_tag="ACHGMT_000883"
     CDS             15590..16051
                     /locus_tag="ACHGMT_000883"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN6834651.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            16077..16529
                     /locus_tag="ACHGMT_000884"
     CDS             16077..16529
                     /locus_tag="ACHGMT_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834652.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            16635..16829
                     /locus_tag="ACHGMT_000885"
     CDS             16635..16829
                     /locus_tag="ACHGMT_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834653.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNGFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            16860..17246
                     /locus_tag="ACHGMT_000886"
     CDS             16860..17246
                     /locus_tag="ACHGMT_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834654.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQAKMTQISHLRLEL
                     EDLRQIEKSIQLNDNQKIVLEWLKSETILTREAPILSVNAFSDKNLLGKLPDKVRKAY
                     KLLACKQEYEVLSAFAQWGLEQEEAE"
     gene            17243..17446
                     /locus_tag="ACHGMT_000887"
     CDS             17243..17446
                     /locus_tag="ACHGMT_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFN6834655.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASNAIRKFGISILNDDRKWEEMR"
     gene            17443..17739
                     /locus_tag="ACHGMT_000888"
     CDS             17443..17739
                     /locus_tag="ACHGMT_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834656.1"
                     /translation="MKIKDGFYASSHGIGGLMLDMPIKNPKTRQKSKFKIGDMVRCKA
                     EEFIYPFRGYVEHVYNHSAIIHIENTMECDKWLAKSKDNLAVARLVDMELINDK"
     gene            17815..18231
                     /locus_tag="ACHGMT_000889"
     CDS             17815..18231
                     /locus_tag="ACHGMT_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFN6834657.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            18492..18629
                     /locus_tag="ACHGMT_000890"
     CDS             18492..18629
                     /locus_tag="ACHGMT_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834658.1"
                     /translation="MDKEIKVTVKLDLTELKELLNKASDQVEQLQETLDEIANFKIQV
                     S"
     gene            19039..19269
                     /locus_tag="ACHGMT_000891"
     CDS             19039..19269
                     /locus_tag="ACHGMT_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834659.1"
                     /translation="MRNYWYISLTNEYPRTIDDCSVRVVRSVQIKKKYSIVEMTREAT
                     PKEIDKCKLLYCGHGFYDEPNIQNNINKNLRD"
     gene            19428..20261
                     /gene="terS"
                     /locus_tag="ACHGMT_000892"
     CDS             19428..20261
                     /gene="terS"
                     /locus_tag="ACHGMT_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFN6834660.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPKRNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            20254..21669
                     /locus_tag="ACHGMT_000893"
     CDS             20254..21669
                     /locus_tag="ACHGMT_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HFN6834661.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            21681..23210
                     /locus_tag="ACHGMT_000894"
     CDS             21681..23210
                     /locus_tag="ACHGMT_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFN6834662.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEYMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKKLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            23128..24180
                     /locus_tag="ACHGMT_000895"
     CDS             23128..24180
                     /locus_tag="ACHGMT_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFN6834663.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            24182..24499
                     /locus_tag="ACHGMT_000896"
     CDS             24182..24499
                     /locus_tag="ACHGMT_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834664.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            24626..25249
                     /locus_tag="ACHGMT_000897"
     CDS             24626..25249
                     /locus_tag="ACHGMT_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFN6834665.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            25264..26154
                     /locus_tag="ACHGMT_000898"
     CDS             25264..26154
                     /locus_tag="ACHGMT_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HFN6834666.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            26177..26392
                     /locus_tag="ACHGMT_000899"
     CDS             26177..26392
                     /locus_tag="ACHGMT_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834667.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            26404..26745
                     /locus_tag="ACHGMT_000900"
     CDS             26404..26745
                     /locus_tag="ACHGMT_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834668.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            26745..27116
                     /locus_tag="ACHGMT_000901"
     CDS             26745..27116
                     /locus_tag="ACHGMT_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834669.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            27109..27504
                     /locus_tag="ACHGMT_000902"
     CDS             27109..27504
                     /locus_tag="ACHGMT_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFN6834670.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            27506..27883
                     /locus_tag="ACHGMT_000903"
     CDS             27506..27883
                     /locus_tag="ACHGMT_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFN6834671.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            27884..28492
                     /locus_tag="ACHGMT_000904"
     CDS             27884..28492
                     /locus_tag="ACHGMT_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFN6834672.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            28492..28833
                     /locus_tag="ACHGMT_000905"
     CDS             28492..28833
                     /locus_tag="ACHGMT_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834673.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGPVVEEAAEVMEG
                     MQNAVDGKSVSEALSRAYSFIADILFEGQFTGEDYRQGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            29075..31030
                     /locus_tag="ACHGMT_000906"
                     /pseudo
     CDS             29075..31030
                     /locus_tag="ACHGMT_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
BASE COUNT        10736 a         4980 c         6096 g         9218 t
ORIGIN      
        1 aacttttatt aacaaatatg ttaatatata agaaaaacct tttaagaggt gatatatatg
       61 caactacaaa taaacgaaga atccttaccg gtatatgaag cattagctag taaaacaagg
      121 atcaagatca tccagctact ttccaaaaaa aagatgaatg tcaaagattt ggcaaaagaa
      181 cttggagtga gcagtgctat cacgacgatg cacgtgaaaa agctagagga agcaaacatc
      241 atcaaaacag aaaaagtagg acaacaaaaa atttcgagcc ttcgtgtcga taaaatcgat
      301 atcagttttc cagaaaaaat attcaatgct tttgatacga aagaaacatc gatacctatt
      361 ggtcactata caaattatgc aattgaacca acatgcggtt tagctacgat ccatgatttt
      421 atcgggaaag tggatgaacc acgctatttt atggatcctc gaaggatgga tgcccgcatt
      481 ctctggttta ccagcggatt tgtcgagtat caggcaccta attttttaaa tacagaggat
      541 acattggaga tgttggaagt ttctgtggaa atttcttctg aatttccttt ttcaaatgac
      601 aattggcctt cagatatcac tttcagccta aatggtgtgg agctaggaac atggacgagc
      661 cctggagatt ttgctgatat ccgcggtaaa tatacgcctg attggtatcc agataatctt
      721 aatcaatacg gcttgctgaa gacgatccga attaccaaac acttgacgaa tatgaatgga
      781 gaacccttgt caaacatcac gatcaacgac atccctaaag agcaagacac ttggcatttg
      841 cgaatcgaag tgaaagacga tgccaaacat gtggggggct gtactttatt tggtaaagga
      901 ttcggtaatt acgatcagga tatcaaatta aaagtttact acagctgata tttttctttt
      961 ctattttata aaacaaaaac agccaatccc tatgaaggga cggctgtttt tgctttattg
     1021 ttatttccag aaatcatcca aaacggaaat tggcaaatgt cgtttatgtt gtgtcttgtt
     1081 ccaccatgct tcgatttttt cttgtgcttg agcgggaact tcttttcctt ctagataatc
     1141 atcaatttca ttatatgtga cacctagtgc gacttcatct gcaatcattg gtttcccatc
     1201 ttcaagatct gcagtcggaa tttttgtgta taatttttcc ggtgcattca atgcttgaag
     1261 caattgtttc ccttgtcgtt tgtccaaacg gaataatggc aaaatgtcgg ctccaccatc
     1321 accaaatttc gtgaagaatc cagtaatgtt ttctgcagca tgatctgtcc ctaatacggc
     1381 tcctgctctt tctccagcta cggcatattg cgtaatcata cgttgacgtg ctttgatatt
     1441 tcctttgtta aaatcgctga ttcccacacc tgcttcgctt aatacttgta cttgtgcatc
     1501 aacagctggt ttgatattta ctcgcagact aacatcgggc tggataaagt caagagcagc
     1561 ttttgcatct tcttcatctg cttgttcacc ataaggaaga cgaacagcga taaattgata
     1621 gttttcatct tttgtttctt cacgcatttc ttccatcgtt agttgcgcca aacggcctgc
     1681 taaagtagaa tcttggccgc cgctgatacc tagtacaaaa gttttaagaa acggatattt
     1741 tttcaaatat gctttcataa agtcaatact tttacgtatt tcttcttttg gatcaattgt
     1801 tggtttgaca cctaattcat tgataatttc tgtctgcagg ctcatttatt ctcctcctat
     1861 gcttctgtat gaagttctgc tacgcttttt ttcattcgtt ccatgatagc cattttatgg
     1921 ttccagcatt cagtagacag atcgactggg tatttttgcg gattcaagtc tcgtttatac
     1981 tcttcccata gtgaatctag attttctttt gtatattctt tgatttcgtt tagtgcaggt
     2041 aattcgtaaa ctctttttcc ttcgataaag atatcttgta aaactgggcg agctgtaaag
     2101 tctcgaacag ttttattgat aaacgtatgc actggatgga acatgaagat agcttcttct
     2161 tctcgagggt cttcatccca taatgtcacg tagtctcctt cagatttccc atcaaaattt
     2221 cgagtgatgc gccacacttg ttttttccct ggagtagtta ctttttctgc attgcttgat
     2281 aacttgatcg tatccatcat ttttccttca tcatcttcga tcgatactag cttgaatact
     2341 gcaccaaggg ctggctgatc ataggctgtg atcaattttg tcccaacacc ccatacatca
     2401 attttagctt tttgcatctt taaattcaaa atcgttgctt catccaagtc attcgaagca
     2461 tagatttttg cttccgtaaa tccggctgca tctaattgtt cccgaacacg cttagatata
     2521 taggccatat ctccactgtc gatgcgtacg ccttggaagt tgattttgtc acctaattct
     2581 ttcgccacgc gaatcgcact tggcacacct gatttcaacg tatcataagt gtcgactaag
     2641 aacacgcagt ctttatgggt tttagcgtag gccataaatg cttcataatc atttccataa
     2701 gattggacta acgaatgagc atgggtcccg ctagctggta tcccaaatat cttcccagca
     2761 cgtacattgc ttgttgcatc tgctccgccg atatatgccg ctctagttcc ccagactgct
     2821 gcatccagtt cttgtgcccg tctagttcca aattccaata gtggatcttc tccgatgaca
     2881 gatttgatac gagctgcttt tgtcgcgatc aacgtttgga aattgaccat attcaacagt
     2941 gctgtttcta ctaattggca gtgcgctaaa ggcccttcca cttggattaa tggttcattt
     3001 gcaaaaacta gttctccttc acgagcagaa cgaactgttg ctttaaattc aaaattttgt
     3061 aaatactcca aaaaaccttc aggatagact tctaatccac gcaagtaagc aatatcggaa
     3121 tcattgaacg tcagattctc taaataattg actaaccgtt ccaatcctgc aaaaaccgcg
     3181 tatccgtggt taaaaggcat ttctcgaaag taacattcaa aaacagcatg taaatcagct
     3241 cgacctaatt cccagtatgt ctgcatcata ttgatttgat atagatccgt atgcagcgtc
     3301 aaactgtcgt ctgggtaagt attattcatc ttgtattact cctcttggca agttttcatt
     3361 tcctacccat tataacaaaa aaaattaata ggaaaagaaa tcttccattt tttctgaaaa
     3421 agcacatgtt ttacggatat ttcttgctaa aaatgaactc catctgctaa aactatcctt
     3481 tcttattggt atttaaccta aacgagaaag aatacttatt ttattcaatg tatccgagac
     3541 acgaagaaat tagcgttttc tatttttctt acttgataaa ataagtagat tttcctacta
     3601 caatcgtagc cgttaagata ccgatgattg aacaaatata accaaatgtc tctacaacat
     3661 aatcaatggc aaaagttttt gaagcaataa tacgtctttc cttttttgct tcttatcccc
     3721 aaagaaacgg atataactta ttatgtattg aatttttttg gaaaggcgaa ttatgacaag
     3781 atataccttg cgttgaaaaa actcgcggaa aaaatatcac acgtttttaa aaagatacaa
     3841 agtaagaaag cagctgtcaa tagaatggaa acaaaaatgg cgagacatca atcagtctcg
     3901 ccttgttttt tattgtatac attagatatg gagcaggtta ccataaagat gattattagt
     3961 cgttattaaa tagaatcctt cgtatttttt tgttctatca ctacaatatc tttatttgga
     4021 cgtttgaatt gtattccggc acgttctagc tcctgtttca ctcctacatg gactttgaca
     4081 tcttctcgtg tcatttttaa aacgtgatga atcgcatagt cgatttcttg ccttgtgatt
     4141 tttttcttcg tattgatcat cacgacttgc tttttatcat atgtgtctac caatagcagt
     4201 tgtgttcgat ctaacgaaaa taatttgtaa taattaatag ccgtattccc aaaaaagaat
     4261 tccactactt taggataaaa tgtcttaata tttaaattac tattcacagg tgtttcgatt
     4321 agcttcatct cattcctctt ttctatatag tcgatttaga tccaatctta agttatgtta
     4381 ccatctttcc tagcattaag gaatcggtta caattgaacc tttaaagaca aaaggattta
     4441 ccttttatca cttaatagta taacataatt tcgtgagaaa aaaatacaat tttttcttga
     4501 ccatctcgtt acgtaaagga ttatgcttac atttgtcagg aggaaaaata atggagtaca
     4561 caataaagaa aatgtcagag atatcaggag tcagtccgag aaccttgcgc ttttatgatg
     4621 agattggact gctaaaacca gctaggatca attcttcagg ttatcggatt tatggcaaaa
     4681 aagaagtcga tcgtctgcaa cacatcttat tttatcgaac aatggcattc aagttggatg
     4741 atatccaaga ggtcttagac aatccatctt ttgaccacca gaaagcttta atcaagcatc
     4801 agcaaatgtt gctggaaaaa cgggcacaaa tcgataccct cttgacaact gtccaacaga
     4861 cacttgatat gtacgaagga ggaagaaaaa tgagtgacaa agaaaaattt gaagggttta
     4921 aacaacaaaa gttgaaagaa aatgaagaat cctatggaca agagattcga caaaaatacg
     4981 gaaaaaaaaa ggttacagca tccaatgaaa aatggatgaa tatggataaa gaaacgtatg
     5041 aagaaatgca aactgttgaa aagcagatgt tatttgatct cactctttac ttaaaagaac
     5101 cagaagatga aagcttagca gaccgtatct tccaagctca taagaagtgg ttctcttaca
     5161 gctggcctga atatcagcca aaagcgcata aaggactagg aatgatgtac gaagccgatg
     5221 aacggttcac tgcatattat gacgagcgaa gtggtcatgg tgcagcaaaa gctttaaatg
     5281 aaattatcca aaaaaaggcg taatctttta catgatccaa caaaaaagta cacgtaaaat
     5341 gacctaataa caaaacagca gacacccaag ttagattttt atgctaactt ttggtgtctg
     5401 ctccattctt tcacaagttt tttcatccta ctttaaatat attcatcgtt gaataaacgt
     5461 ataaggtatt ttcactttat cctttttttc tgtaagaaac aattgaaatg ccatttctcc
     5521 gcaagcttcc agatggtggt cgatggttgg gaaaccaaga agctcgctga tcagcaaatt
     5581 atcgcgtcca atcaccactg gtgtcttatc ttgcagatag ctttgcaaga tgcctgctgc
     5641 aaccatatcc gaatttgtaa agacaccatc aactccaagc tctttaaaaa agaagcctgc
     5701 ttgttttcct ttctctgcat ctatacaatc ccaaaaaaca agtgattcat caaaagatgg
     5761 aaaaagttga tgacatagct gcaaagtgat ttttgagtta taacttagcc tgccacttcg
     5821 ccccaacgtg atgcccaatt gctttatacc ttgctgcttc aaataagtaa gaccttctgt
     5881 aattgattgt tctcgatcaa taaagacaca gcttgcctgt tttcccttga tttcttcaca
     5941 aaaaactaga ggtccatatt tttgatatgc caataaatca tctagtgtat tggcacgaga
     6001 cgtaatgatc aatccatcga aagctttagc tgcaaattct tcaagatacc ttcgctcttt
     6061 ttctttatga tagcccgtcg gtaaaagtgt cactttgtaa tcataatcaa acgctgcttc
     6121 aatgatccca ctgatcaact gatcaaaata cggatgattg gtataaggca gaatcactcc
     6181 aatgcttttc gtttttccat aacttaactg tctggcattt gcattaggga tataatcgac
     6241 ttctttcatg accgcaagta tttctttcct tttttcctca tctacatatg gatgtccatt
     6301 gattacacgt gaaatagtag aaactgaata acctgtgagc tttgcgatat ctcgaatgtt
     6361 tgccactgct gcttcgccac ctatacaaaa agattttctt gtaaatagtc taccacagct
     6421 ggaccgcatt caaaaaactg atacattttt ccttcatgcg aaaaactgat ccgctgcccg
     6481 ccccaatata tatcttttgt caaattttcg atttcattca ttggtatttc ttttactata
     6541 ttcctattca acccttgaaa actgtacaac gttaatacgg atcccttgaa ctcgacaaac
     6601 gcataaaaat aattgttcgt tgtgccatct gttatctcta cataagtatt cattgcaagc
     6661 cctccttttg atactttgag tatagtgtat gaaacgcgtc tcatagcaag gagtttgttt
     6721 tgccaaagaa aagttttatt cctctttcat tattaccaaa gtctcttatt ttaaattgaa
     6781 tacattataa tgatactgta aatatatctt tattattagt gaatggagtc aagtccacaa
     6841 tcttgtgtaa aagttcaacc tcatcaaaag cacaatgttt tgcgttgtga acctaactgc
     6901 catgcctttc tttctgagga gagaggtcag gagcagtagc gcagcgactc ttgatgtctc
     6961 tcctcagaaa gaagagactc gcacaggttc atggtgctaa atgttattcg aattaaattc
     7021 taaatatttt cgtggagaag ccagccactc agtgtctttt tccatcaaaa gtgttccaat
     7081 tatccgagtg atggaattga tatttggaaa aatccgaatc actcgttctc tccggcgaat
     7141 ctcttcgttc aaccgttcca acacattggt tgtacgaatc cttcggcgga tattttcagg
     7201 aaaagacaaa atttgaatag catcttcaaa gccgttttct aaaatgtcgc aagcagcact
     7261 taattttgaa tcacaaccat atttttccaa gaagtgctct tttctttcac gtgtcaattc
     7321 aagttcagag gctttaaaga tactcttcaa ctcgtctttt acatcagaag aaactttctt
     7381 tggcagctta tcaaaaatat ttctcaaaaa gtgggcttgg catctttgcc agctcgcacc
     7441 taaaaagttc ttgcgaacag ccttaactaa ccctttgtgt gcatcagaaa tgaccagttt
     7501 tacaccgaac aaacctcgtt gaacgaacga ttgaaaaacg gtatcccaat tatcttccga
     7561 ttcgccgtct tgaacatcaa aaccaagaat cgtacgtcgt ccatcagaat caatgccgat
     7621 accaatatat acaccttttg agacaatacg atgattttcc cttactttca tatacagggc
     7681 atcacacatg aaaaatggat attccgagcc ttctaggctc cggtttctcc actcaaaaac
     7741 aaatggatcc aactgtttca tcagatttga aacgaatgat ttagaaacag tcgcaccatc
     7801 cgttagcagt tcaactgttt tagttacttt gcgagtggag acaccagaaa tgaccatttc
     7861 aatcatggca gcgatcaaag ctttttcgct tcgctgatat ctttcaaaga tagtaggact
     7921 aaacttaccg tctctggttc gtggaacatg aagttctaac gtaccaattc gagttgtaaa
     7981 cgaacgatcg tagtaaccat ttctttgtga ttgccgttct tctgaacgtt cataggcatt
     8041 tgcctgaata tattgatttc gttgttcttc catcagctga ttaaacatga tagtcataag
     8101 tgtttttgcg gtatcattcg caccgctttc gtttataatt gcttgaattt cttcgctttc
     8161 aaaagtaaaa tgtacttgag tcattgatat cagtcctatt cgagtatgtt ttctgcaaaa
     8221 aacattgtac tcctaactga gatcaatggc ttttttgatg cttcactttt acacaattat
     8281 atggacgtaa tcaattaata tcatttttta acatagtaat ttgtttattt aaatctgata
     8341 gtgtttcttc cttacttaat ataaatcttt ctctatcatg taatttattt agtttttcat
     8401 catttttttg ttctaattca taagcttctt tttttattgt gttagctttc gacagtttag
     8461 tgattcctat aattaataaa ataagtccaa tcaataataa cattcaaaac tcctcatttc
     8521 tttgatataa ttattgtaga gtttactcag aaatgaggaa agagtccatg tgaaagcatg
     8581 ggctttttta tttttaatgt tgtaatacta aatgttcaag attatgagga agccctaaat
     8641 attcaataat ttggaactga ctcatatcag ataaatcttg ttcatctatt gttctagtta
     8701 acatttcaat tgcaaacata ttagcttcag cttctatacc ataaatcata caactaaggt
     8761 tgtttgctct aaaagctgct gtgcaaatac ctttatggag tctaacgtgg gctatttcat
     8821 gccataatac aaatcttttc atagaaggtg ataggttttg attaattaat atcacataac
     8881 atcttttatt gtgtatcgtt tgtcctaatg ttttattccc taaatcagtt tcaattaaat
     8941 ctatatcaga atatttgatt attttatatg gattaatcgc atcagatcca aactctacaa
     9001 ataatttttc tatttcttct aatatccagt atttgtttaa atgcatacca aagcccacct
     9061 attgacgata ttttttagga gtgaattttt tctttgcttt aactttggtt aaggcaagtg
     9121 caagttgaat ggcatcgtat aatttttcct tatcttcatc gtctaaaggt tcaccataat
     9181 aatttgtttc agcatctgaa ttcaagcctt caagtaattt ttcagcttgt aaagcaatat
     9241 cttgtttatc tttttctgtt aaatcataat aatggcgttt atcagtacgt cctaaaagat
     9301 aatcagttga tacattaaag taatctgcaa ctttttgtat tttatctcca gatggagtac
     9361 ttttattcca tctagacatg cttccatttc cgaaatttaa ctttctttca agttctgcca
     9421 acgtcattga acgttctttt aataagattt ttatacggtc taatagcata ggatatgcat
     9481 tcctttcaaa ataccgacaa aaaaagtatt tttctgcaaa ttatctattt acaaatagag
     9541 aatttgcagt tatactagtt ttgtaagcaa gatatttacg gcatacaaag actaaaagca
     9601 ataaactaat agtctcccca gacatcagta agctcgcttt ttgtgcgctg tattttgtat
     9661 gcttacataa tagcatattt gcagtttttg tcaaacttat tagacaaaaa tattagaaaa
     9721 tattcgataa gggggtatga agcatgactt taaaacagca agtattaatt gctttagtaa
     9781 agaaaggttg gagtcaaaga gagttagctc gacgaatggg tatatcaatt acttatttac
     9841 gagatatctt catcgaaaaa agaaaaccta aggaacgttt aaaacaaatc gaagaacttt
     9901 tggatatcaa gttagaagta gattctaatg atcagccatc agaacaggag gcgtaaaaat
     9961 ggtacaacgt ttgagcaaag cggagtttaa agagaaatat aagtattctg aaagtactta
    10021 tcaaagaaga atgaaggaat ttaaaaaatc acgttttagt gaggggtata acgcagttac
    10081 ttccaaagaa ataacaattg atgtcgattt atatgaacag tttagaacat ggcgttctaa
    10141 aaatcgattt agagttagag ctgtttgtta atagctaaaa aatctaaatt caccacttct
    10201 gaaaagagga gttgctgctc tcctttcaag tttgccaaag tccacgtttt ttcattttgg
    10261 caactcctct tcttggaggt ggtgacggcg agagacgaag gagggatgac atgaaggaag
    10321 aatcaaaaat agggattttg aatgagaaca attacacaag attaattatt gaaacagaag
    10381 aagggaaaaa agtagcagaa gttacattga ccgatgcaac tcctgctgac ggttatcgta
    10441 ttcgattaac tcctaaatat gattaacctt ttggtgggtg tggatcgttt ccgtgactat
    10501 cacggctttg aatttttcca tctctgccat gaataaatag ctcagatcct tgatttttag
    10561 aaatctctct agcgattttt actgcttctg attttgtatt agtatgtgca gtcgctttag
    10621 aatttccagc acctttgaca ttccaaccac cattggaatt aggtacaaca tgttgatttt
    10681 ttcccatagt tttcacctcc atttctatag aaatatttta tcaaatagaa atggagaaat
    10741 tcaataaacg aaaggagaag tgatatggca tataccttgc aacaagaaca tcaaattctc
    10801 ggtttgatta aacaacgcag gaaacaatta caagatgacc gtgcagcgct tagaaaagcc
    10861 gatgagctat cagataggca agctgaacta attgcttctg aacttgagga tttgagaatg
    10921 ctagaaataa aaaataggga gattagatta tgaagaagac agacacactt tttataggat
    10981 tcattttggg gttattagtg attgtagcgc accaaagtat tatcggggga agcttgttcg
    11041 cagcattgat ggttttaatc aatctgcttg attcaaaaga aaggagcaac tatggcacga
    11101 gaagaagcgc taaaaatcgg taaagtgatt gctgataatc ggtggggaaa tagccgccct
    11161 attattttaa gcaagcaaca tatcgaaaag caaaaagcat ggcaacaaat aaaaaagtga
    11221 ctctgccagc cagcaaagag tcacatacaa atttataaaa ctaggagaat tatatcacat
    11281 gaataagaaa attgaaaatc ttatcgaaga attgaaacgt gaatgccaaa agcaaggtgt
    11341 ttctattatt tgtacagccc aaaaagaagg ggaacttaaa agtctcgttt atggtgagac
    11401 aacagaaatt ttactttgtc ttgctatgca agaagaacat ttagatgaaa atctcccatt
    11461 gtcagcacat attatgcgta gaattgctgt ggatgcttac gaacaagcga aaaatgaaga
    11521 agaaaatcaa ccttcaaatc atacttttgt tattaataac aaggaagatt tagctgatgt
    11581 gatgactcgt attctcaagg gggaatttca atgatgaatg aaaaaacaat tgatctactc
    11641 attattttat ttttcacgtt agtgattgta gcgaaaagtt atgtaatctt tggcttgtta
    11701 ttcgcaatta ccatgcttgt gctttgcgtg ttagcaagca gaaaagaggt taaacatgaa
    11761 cgagttagca aatcttgaca actatttaac tgatcctgat tatgctaaac caccatatga
    11821 agcaccaatt gatgaggagg atgaagatga gtaaatctac cttagaaatg agccatcaag
    11881 aatggcttga agaccgtaag aaaggcatcg gaggttcgga tgttggaaca attttaggat
    11941 tgaataaatg gaaatctcct tatcaactat ggctagaaaa aacaggacaa gtagtacttg
    12001 aagaatcagg aagtgagccc gcttattggg gtaatgtttt agaagaagtg gttgctaaag
    12061 agtttcagga acgtacaggc aaaaaagtac gcagaagaaa ccaagtattt gaacatccat
    12121 tacatccatt tttaagagca aatattgatc gggaagtagt gggagaaaat gccattctgg
    12181 aatgcaaaac agctaatcaa tttctcggta aagaatggga aggcgaagag gtaccgctta
    12241 gctatctctg tcaagttcaa cattacatga acgttctaaa caaagactat tgttacatcg
    12301 ctgtcttaat cggtggtcaa aaatttatct ggaagcggat tgaacgagat caagagctga
    12361 tcgatacaat cactgaacaa ttagtagagt tttgggaaac aaacgttctt ggaggtatcg
    12421 agccaattat tgatggtagt caagcgacag ctgatttctt gaaagagaag tatgcagatg
    12481 ttgaagacgt tcaaacgacg ttaccaattc atttcgatga actagttgaa cagaaaaacg
    12541 aactcaaacg gactaagaaa gaaattgaat cagctatccg tcaagtagac aacgagatta
    12601 tcagcgagct aggtaaacgt gaagccagta tcggtatcac tcaaaaaaac atcatcagtt
    12661 ggaaatttgt ccgtacgaga cgtatgaact cgaagaaact agcagagaaa tatccagatg
    12721 tcgcaaatga tgaagagatt tataacgtta ctgaatcaag aaggctaacc gaaagggaga
    12781 tcaaataata tggcaacaaa tgaatcgtta aaaaatcaat tggcagcaaa gccacagaaa
    12841 caagttgcac caggtcaatt aggacttaaa gctttgatga atacaccaac aatgagaaag
    12901 aagttcgaag aggtgcttca tgacaatgct aatgctttta tgtcgaatgt tatgactctt
    12961 gtatctaatg acagttatct tgcagatagt gaaccgatgt ctatcatgag tggtgcgtta
    13021 actgctgcaa cattaaatct tgggctagat aagaatttag gttatgcata tttagttcca
    13081 ttcaatagta aaaacaagca aacaggaaaa tgggaaaaga aagctcaatt catgcttggc
    13141 tataaaggat atatccaatt agcccaacga tcaggtaaat acaaagcatt aaatgtgatc
    13201 gaagtttacg aaggagaact aaaaagctgg aaccgactga cagaagagtt tgagtttgat
    13261 ccaaatggta gaacgtctga tgaagtcatt ggatatgttg gctatttcga gttactgaat
    13321 ggattcaaaa aaactgtcta ttggaccaaa caagaaatcg aagctcatcg gattgctaac
    13381 aataaagatc gcgataagac aaagttaagt ggtgtgtggg catctgatta caatgcaatg
    13441 gcacgaaaaa ctgttttgag aaatcttctt tctaaatggg gaatcttgtc catcgaaatg
    13501 caagaagcca ccacatcgga tgagagagtc caaagggttc aagaagacgg cagcattatt
    13561 gctgaaacag aagttgaaga agatattcct gaaagaaaag aagcagaggt tatatctgaa
    13621 gaaaatgaag atgtacaaac tggattattt gatgcatcta atccgccgtt aaacaaataa
    13681 tgagggagct ttctcccttg cttttctaga ggagaaatac gaatgaacac aggatatgtc
    13741 aaattacatc gaaaagtgat gaattcattc gtttggacca atccttatat gtataaatta
    13801 tggaacttgt gtttgatgaa agcgagtcat gagaatcgca agatactttt taatggaaaa
    13861 gagatacaag tgaacagcgg agaattcgtc acagggcgcg atgctatcac atctgagatg
    13921 aacgaaggcg tgaaacgtga acatcaagtg aacagcggtt ctgtatggag atggctaaaa
    13981 caatttgaaa aaaacggaat gttgaacatc aaatcaacta cgaaatacag cgttatatca
    14041 ataaaaaact ggtctttgta ccaaagcagt gaacaacaaa tgaacatcaa acgcacaaca
    14101 agtgaacaac aagtgcacac aaacaagaat gaaaagaatt ataagaatga aaagaataat
    14161 aaaacatcat cgtcgcaacc acgcaaaaag cgtgtttacg acaccgactc agtttactac
    14221 attctcgcgg aggagttatt caaacagatt tgtcagaatc aggaaatcaa aaagccgaat
    14281 ctgcaaagtt gggcagataa cattcggaaa atgatcgaga tcgacaagcg gaccgaaaac
    14341 caggtacggg gaatgattga atggagtcag cacaatgtgt tttgggcatc gaatatttta
    14401 tctgctaaaa aattgcgaga aaaatacgac acaatggcag cgcaagccaa tcgtgattat
    14461 aaaacaaaac aaactaaaac gcttgaatac gagaaattta gcacagatga gttgcctatt
    14521 tgagaggagg cgtaggcatg gaaactgttg gggaaatcat ggaaaagctg atacagaaag
    14581 tgcttgtcca acgtggcgaa tgtcctgaat gcggacagcc tttgtatgga tggcgtacga
    14641 agaaccctga tggctcagaa cgttgtaagc caacctgtat gcaatgtggg tataaagcat
    14701 tgcgagttca ggaagactta caaacagaac gaatttacaa cgagagcctg aaagcaagag
    14761 caatcaattt tttcaaaggt ggttctgttg tgcctaatca agcgttgttt gattgcacat
    14821 tgcagaatta tcaaattgtc gatcaagaaa caagacaagc ggttgaagta accaaacgct
    14881 ttgttaattc agtcttgtta ggaaatccaa gtcaccttgt tttaactgga aaacaaggaa
    14941 caggtaaaag tcacctagcg atggcagcgg cttgggaagt cttgaagcga tcaaactacg
    15001 ataaaaaaat cttatttatc gggttacagg aaatgctgga tcaaatcaaa ttttcgtaca
    15061 acaatcctga actcagaaaa acgattgagg gatcgttgat tgcagatatc aaaacagcgg
    15121 atttagtcat cattgatgat atcggttcag aactaggaaa agatgcatca gatagtcgag
    15181 cgtttggcat aaacacgcta aattcgttct tggatgcacg acagaaccta gcaacgatta
    15241 tcacaacgaa cttgcttggt gaagaactga aaaaagctta cggtacgaga acaatatcaa
    15301 gaatgtttgt caactctgat ggatttacga tggtattttc tcaaacagca gacaagcgca
    15361 taaaaccagt caaaggagcg atcgcatgaa tttgactaaa caagaaaaaa gaatctgtcg
    15421 tcagataatg ttgcaaatta taggtgatcg tgacatggac gaattatctc aacaagagag
    15481 tcgaatggtt cggaagataa gtaagctaat tatttatcgg gatacagaaa gatttctcac
    15541 tgttttaggt aacgtctacg agaatccaga gttattggag gtagagtaat tgccaaaaat
    15601 attagatgca tgttgcggta gtagattgtt ctggtttgat aagaacaatt ctgacgtaac
    15661 atttatggat tgtcgtcagc agtatgaaga attatcaaca ggtcatgtga tcaatgttga
    15721 tccagatgtg gtcgcagact ttcgcgatat gccttttgaa gatgatgtgt tcgatatggt
    15781 tgtatttgac ccgccacact taagacatgt cggcgaaaac tcatggcttg ccaaaaaata
    15841 tggaaaatta gacgaacttt ggccagaaga catcaggcaa ggttttgcag agtgtatgcg
    15901 agttttacga ccttcaggaa cattaatttt taagtggaac gaggaacaga taccactttc
    15961 tgatgtgtta gaggctattg gagaacagcc attgtttggt aataaacgaa gcaaaacaca
    16021 ttggcttgtg tttatgaaaa aattcagcta acggaggaaa caaaggagga aaagtaatga
    16081 agattgtatt aaataaatgt tttggtggct ttgggttgag ccatgaagca aaaatggaaa
    16141 tttttaaaag aaaaaatatt gaagtattcc catacatcaa taactttagc tatgactcag
    16201 atgatgaata cacgagatac acaggccaaa agctaggttc gatggatttt atctactatt
    16261 tcaaaaaaga cccgaaaatc gacaaagtta caggaattta tagcgaaatt gaccgactgt
    16321 acggaattgc tgatgattcc agtttttcaa gtgattcaaa cagaggtgat aaggacctcg
    16381 ttgctgttgt cgaaaaacta ggaagtgagg ccagtggacc atatgcaagt cttaaagtcg
    16441 ttgatatacc cgatggggct gagtgggaaa taagtgatta tgacggcgtt gaaactgcac
    16501 attatggttt tcaaacaggt agtatttagt ccactaatga caactaaaga ggaggcagaa
    16561 ggatgagtga agatgaatac tttgaacagt agtctattaa cgacagagat ttttaactgg
    16621 aaaggggtgt agtcatggac gcatcggaag tagtaaataa aattaatagt ctcaaagagg
    16681 ttttcggaga cgtcgaagta gtgataaacg cacaaggcta tgacaacgga ttttttaagc
    16741 agattaccga cattagcatt cagcaaggaa tggaagacga agatggaaac tttatcgatg
    16801 aagtagccat tttagtgact tgcgagtaag taattgacag ctaaagagga ggcagaagga
    16861 tgaaaaaatt gcaccaactc atatctgaga aagaaagcga actccaaaat ttagaagata
    16921 gtttgggatt ggggtttcca attgttgagc aagctaaaat gactcaaatc agtcatctac
    16981 gacttgaatt ggaagattta agacaaatag aaaaatcaat tcaactcaac gacaaccaga
    17041 aaatcgtgtt ggagtggttg aaaagcgaga caattttaac tagagaagca ccaatattat
    17101 ctgttaatgc tttttctgat aaaaatttac taggaaaatt acctgataaa gtacgcaaag
    17161 cttataaact attggcttgt aaacaagaat atgaagtcct atcagcattc gctcaatggg
    17221 gattagaaca ggaggaagcg gaatgaacaa cagacatcgc agaataacaa aactaagaaa
    17281 acaggaactg aatgtactaa agacaaagtt tgaaaaagaa tatggaattt cagcagaaga
    17341 aacatataaa gtggcaagtc agtttgttgc tgatgcgagt aatgctattc gtaagtttgg
    17401 gatttcgata ttaaatgatg atcgtaaatg ggaggaaatg agatgaaaat aaaagacgga
    17461 ttttacgcta gtagtcatgg tatcggcggt ttaatgctag acatgccgat aaagaaccct
    17521 aaaacacgtc agaaatcaaa attcaaaatc ggtgacatgg tccgatgtaa agcagaagag
    17581 ttcatctatc cgtttcgtgg atatgtagag catgtctata atcattcagc aatcattcac
    17641 attgaaaaca cgatggaatg cgataagtgg ttagcgaaaa gcaaagataa tttagcagtg
    17701 gctcgtttgg tggatatgga actaatcaat gacaaataaa aaagccggat cgctccgact
    17761 gattcaataa atccaacaca tttattatat cacataaagg agcggtttga cttgatgcaa
    17821 ttgttacgag aggtagattt caaacagaca agatgtaatg cgagagatgt gctgaagaac
    17881 tttcggcgtt tggagcggat ggcaggtcgc tctttgatag atattaagtc gccgattatt
    17941 acggatatgc cgaaggcacc gaagcacggc aataaggcag aagacgcgat cattcagatg
    18001 atggatatag aagcggagag agacgcaata ctagcggctt tgatggctct tagtctgatt
    18061 agccgtcaga tactctacta cagcttctgt gacgtaaaca agcactctaa ttatgaaata
    18121 gggcaattga tacgaggata cggagagaag aatgtagaga agttgaaatc catcgcatta
    18181 atcgaatttg cagaagcata caaaaaaggc gtgttagttc agtatcgttg attttgtagg
    18241 gtttttgtag ggatagtgta gggtttttga gcggtttaac gtgatattat ggtagtgtcg
    18301 aaagattagt gataggtctg agacaaaata aaatgtaagg gaggaaatct ccctcatcgt
    18361 tttaacttaa gcttcgatag acagcaacgg aaatattaag aataaggatg tgaatttcaa
    18421 ctccttctaa attgttctta ttatctatca tccgttgttg tctattaatt tatgcatcgg
    18481 aggaaaaacg aatggataaa gaaatcaaag taactgtcaa attagatttg actgaactaa
    18541 aagaactgct caacaaggct agtgaccaag tcgaacagtt acaagaaact ttagatgaaa
    18601 ttgctaattt taaaatccaa gtttcttagc agtatatttt ttagcagctg cactcataaa
    18661 atcataccaa gtatcagaaa aatataactt aactaaataa ttaaaaaagg tgggtaaata
    18721 gaaatgacgg cgttaataat ttcaattttt gcgctctgtc ttaatgtcta tatgattgga
    18781 tttaaaaatg gacaaaataa aaaatagtag cagccgagaa taattttata gtgtcactgt
    18841 ggcggaaata gaagacgcaa aggtggaggt aggaagcgta tggcgcacta tcgggacctt
    18901 gtacaaaact agaaaggact agtacgtgtg tggtgcgagc cctagggagg aacaggataa
    18961 ccgcctgtgt gtaggttgct attacatatt agatcactct ttgagtggtc tttttattta
    19021 cataaaggag gctacataat gagaaactac tggtatatat cgctaactaa tgaatatcct
    19081 cgaaccattg atgattgttc agtgcgtgtt gtgcgttctg tccaaatcaa aaagaagtac
    19141 tccattgttg aaatgaccag agaagctacg ccaaaagaga ttgataaatg caaacttctt
    19201 tattgcggtc atggtttcta tgatgaacca aacattcaaa ataatattaa caagaatttg
    19261 agggattaga atgcaagaaa tggcttaaaa atctccaatc aggacaaatc aagttagaca
    19321 aagtttccga tttaaagata ttgattgaag ctgatttaat gctaaaagat attgaaaatt
    19381 agaaaacaaa actcaaccta agaagattgc gaggtggtgt gtattgaatg gcaagacaac
    19441 gtgatccaag acgtgatgaa gccaaaagaa tttggttaga atccaacgga gaaaagcagt
    19501 taaaggaaat tgcatctgaa ttaaatgttt cagattctca ggttagaaaa tggaaatcgc
    19561 aagacaaatg gagcgctgaa ttgaaaagta acgttaccaa tggcaaaagt aacgttacta
    19621 atcaaggtgg cgctcctatt ggtaatcaaa atgctaaagg taacaaagga aacagcagag
    19681 catcaccgcc aaagagaaat aagaatgctc ttaaaactgg tgagtatgaa acaatatttt
    19741 ttgacacgtt aaccgataat gagaaggaca tctattctag tttgaatgat gatccttctt
    19801 ttgctttgtc cgaagaaata cgacttctta aaataagaca acttcgaatg atgaaaagaa
    19861 tccaacaagc tgaagctgga ctaaatgatg aagaagtcga acgattgcag cagctaagaa
    19921 agattaaaac tccgattgaa aaaaatggta aaaagctaga aatcaagcgt gaggttatgc
    19981 aagatgtgca gattagcaga aaaaaacatc gcaaaattga tgatattctt tcaattgaag
    20041 attcattgac tcggattagc aaccagttag ctaaagccat caagcaaatg aatgaacttt
    20101 atatgaatga atacagaact gatttaatta aagctcagac tgataagatc caagctgaga
    20161 caaatgaaat tggcggaaat aattcaggtg aagaaataga agaatggaaa caggcagttt
    20221 taaatgccgc aaacaaacgg gcggtgaaag aaaatgaata atgaatttat tccttttgct
    20281 gatattggtt ctgccattga ttattactat gataaaccag tagctttttg ccaagatatt
    20341 ttgcatttga atcctgatga atggcaagaa aatgttttaa atgatttagc tgaattttca
    20401 aaggtttctg ttcgttctgg tcaaggagtt ggaaaaacag cattagaagc aggagcaata
    20461 ctttggttct taacgtgtcg accctacgct aaagtaatag caacagctcc gacaatgaag
    20521 caactttacg atgtactttg ggcagaggta gctaaatggt taaatgatag cttgatcaaa
    20581 aacttactga agtggacaaa gaccaaaatt tatatggttg gtgattcaga gcgttggttt
    20641 gctacggcta gaacagcgac taaaccagaa aatatgcaag gttttcacga ggaccatatg
    20701 ttgattgtgg tagatgaagc ttctggtgtg tctgatccaa ttatggaagc tattcttggt
    20761 acgctatcag gttttgataa taagctgttg atgtgtggaa accctaataa tattgaaggt
    20821 gtcttttacg attcccacaa ttcagaccgt gataaataca gagttcataa agtatcaagc
    20881 tatgatagta aacgtacaaa caaagacaat atagaaatga ttcttaaaaa atatggaaaa
    20941 gaaagtgatg ttgctcgtgt ccgtattttt ggagaatttc ccaaaggtgc gttggattca
    21001 tttatcagtc ttgaaacggt tgaattggct acagaaaaac aaattagtga ttctttagtc
    21061 aataaaacaa cggttgctca tattggtgtt gacgtagctc gatatggtga tgattctacg
    21121 attctctttc ctagaattgc taccagggca ttggagtatg agaagtattc aaaacgtagc
    21181 actatggaaa caacaggata tgtcatcaac atggccaaga atctaatgag tcaatatccg
    21241 agtattgata aagtgatgat taaagtcgat gacactggtg tcggaggtgg tgtaaccgac
    21301 cgcctagaag aacttataga agacaagcat tatccttttg aggtgtttgg agtgaataac
    21361 ggttcaacat cagaagacga tttttacgat aatttaggta ctcaactatg gggaaacatc
    21421 aaggaaatgt tagaagaaaa tatgacagca aatcttaacg gagaacagcc tgttattgaa
    21481 ttgccttctg atagttcgtt aatcaaagaa ttaagtactc gcaaattcaa aatgacaagt
    21541 agaagtcgta tacgtttaga aagtaaagat gatatgaaaa agcgaaatat tggtagtccc
    21601 gatattgctg acgcactggc tttagcgttt tatgagccac caagtcacta tcaatttatt
    21661 caattttagg aggtgagttt ttgattagta cagtattatc tttagagaga tataagaagc
    21721 tacgtgttaa atatgcaaca caaattgaag atggaatgtt tgatccgaat ggttttatag
    21781 aatatatgaa accatttttt gctgatcgcg aaagaaaata tctagcttat actagcgaaa
    21841 agaatgaaat agacaataga ccaaagccta acactgatat tgtaaaagtt aataataaac
    21901 ttcatgctgg tatgtattca attgtcgtag accaagcagt caatcatttt actggtatac
    21961 ctatcaaatg ggattacgat gtatcggaac aaaaaagaac actcattcaa agattaaaag
    22021 ataaattctt aaaaaatgat atagaacttc caacagttcc agaagctttt aacaaattaa
    22081 caagcaatct tgattccatg agattcgcaa tgcttgattc tgaaactgca acttttcaag
    22141 gagcttgtgg agtagctttc cgattgttag agcctgtgga agaagatgat ggttggaagt
    22201 taagagcaag taatattgaa ccttggagag cagaaagata tggaaatgcc ggaatctata
    22261 tcaaagaaaa gtatgactct taccagaaaa aattttttca agaaatgaaa gttatgacaa
    22321 gaaataaaat tctgacatat gcttgctatg gtgatttgaa ttttgttaca agcggaacgt
    22381 tcaaaaaaat tgatgagacc gataaccctt tggggacgat taccttggca gaatttaaaa
    22441 ataatacgaa tcgttattgt gattttgaag tagctgagga aattggagat gcaattgatc
    22501 gtgctttgtc tgatcaacaa aacgaaatcg aacaatttaa gcttgcgtac atgcttgtta
    22561 ctggcacaac aatgagcaaa ggcactgcta aagaaatgat gaatcagcta gggattatca
    22621 acttaaaaga ccctactgca aaagctgaat atgtcacaaa aaaattagca aaagatttca
    22681 atgaatatca tatggatcta ttgaaaaaac agttttacac catttgtaaa gcaatcgatt
    22741 ttaacgatga ggtgtttaaa tcaaacagtt ctggagaagc tcgcaagtgg caaatcatta
    22801 gcctagaagc gaaaacaaat actaaagagc aatattttag agaaggattg aaagaatgcg
    22861 cagaaacaat tgctgctttt cttaagtttc atgacaaagt agaaattgag cctgaaaaaa
    22921 ttatttttac tttctctaga tctttgccaa ctgatttaag ttatttagct gaagctttac
    22981 ctaaacttgc accatacgta tccaaacgaa ctattcagag ccaaattcca tttgtaacag
    23041 atgttgatta tgaaaatgaa atgatggaat tggaaagtgg aagtgcttat ccagatagtg
    23101 aatacaattt tggcggaggt ggcaatagtg acgatagaaa cgaagtattg gaccaaacgt
    23161 cgagaactgg aggatcaagc aaggctcaaa caagaaaatc agacacttaa aaaattaact
    23221 agtgtatttc ctgaagcact gaaagagata caagcaaaac tattatcaca agctgactta
    23281 cacaatatca cttatccaga aatgatggag ttttacagta caagtaatca gaaaaaatat
    23341 cgtgaatatg tggaaaaaaa ttataagtca ttaaaaatgt atgatgcaaa atacaaagag
    23401 tttatcgatg aattttttcc accatttgac tatgcaaaag tcaatcgctt attacaaata
    23461 cgatcagatg tatttaaaat tcttgcagaa tatgcgatgg atgcagatgt gaatcaatat
    23521 ttttctgatc gcttagagga aattcttcaa agaacatatt cttctaatgc taatgttttt
    23581 gttcaacttt taaacgttga tataccaaat tatttaccag aaaatgaaat taaacattat
    23641 ttgaactatc catggtcagg aaagacattt tcaagaagac tttggggaaa tatttcatcg
    23701 cttgaacaga aactctctaa tgctattgta aaaagtgttg ctagtggaga aggcgttata
    23761 cacgcattaa acaccatgag attagactca gaaatttgtg acatgtttaa gttagaagaa
    23821 tcaaagtata acaaagcgat agaaaatctc gttcgaacgg agtatgcaaa atttgcacaa
    23881 gatggtattg aaaaatcata tttagaaaca ggtattgagg aatacaacgt attgactgca
    23941 aaagatgaga gagtttgccg gatttgtgga ggaaaggcaa gtaagaatcc ctataaactg
    24001 aaagatgctg tcataggtga aaatcgagca cctttccata gtcgttgtag atgtacggat
    24061 gttcctaatt taccaaaatt aggaaaggat attgatgaag aatatgaccg tttatttggc
    24121 gatttattag atgagtttgc acatgattct tttggaatta atttgaaacg gaggaagtag
    24181 aatgaaagat ttttttgaag cagtactaac aattaatgta aatgctgata ttgcagaagc
    24241 ctacaaaaca gctattgagt ctgagaacca tcctaatggc ttgagagacc attggaatgg
    24301 caattatgcc tacgtggtta ttggcgatca aactgttaat tatcaagata atactccagt
    24361 tgataagaat accgttaatt taacgattca attattatct cattcattac caaatttaaa
    24421 agaaacagtt gattggtatg aaaagatggg atgtattgtt gttagaactg actacaaaga
    24481 aggaaagtct agtaattagg cttttttatt ttgtccgaaa tgacgttaaa ctagcgcaat
    24541 actgggcttg gttgaatggt ggggcgcaac tattaaaact caaagcaatg cggggcgtga
    24601 aaacgaatcg tggggcgaaa ggagaatgag catgaaacac aaatcattaa tgccaatgaa
    24661 tttgcaatac tttgccgaag gtgacgatca aaagttttct tttgatgact tcaaatcttt
    24721 tgtggaatcc aatgaagaag ctcaaaaatt tattcagtca caatctcaaa gtgttgccga
    24781 caaacaattg gaagcttgga aacaaaataa cttagataaa attaaacagg ataccatcaa
    24841 ggaatatgag gaatctaaga aaaacaagtc acctgagcaa attcaactgg aaaaactaca
    24901 agctgaattt gaagcagaaa aagcgttgcg tgtgacaagt gataataaag catttgttgc
    24961 agaacaaatt gctggattag aactagatgg agagttaaaa gagtctattt ctcaatttat
    25021 gctaaataat cttgttagtt cggatacaga tttcactaag aatgctgttg aaggttttac
    25081 aagtgttttg aatgcaatta atgagaaaca tgcagatgca ttaaaagaac tacaaatgaa
    25141 gtctgcattt ggtggaactc aacaatcgaa taaccaggtt cagcagaaca atgaaacatt
    25201 aacaaatcca gaagaacaat taggacaaat tcttcaacaa tttaactagg agagtgaaaa
    25261 attatgaaaa aaacatcttt aaataattta gagtatttgg atatttcacc agcgattaat
    25321 gctatgcaag taccaaatac acctttttta agctatttac ttggtgctgg aaaaacagag
    25381 ccagcaaact cgacagaaat taaatggcgt gaatatgata tcaacaacga tgattcttct
    25441 gaaaaacttg agggcggaga atatccagat gctgaatcag gtcgaacttg gtttaacaac
    25501 tatactgaaa tttttagaaa atcaacctct gtatctggta cattagatgc tattaatgtg
    25561 aatggagtcg gaaatgaatt aactaatcaa gtagcactac gtggtatgga aatgaaaatt
    25621 gatttgaacc gaaaattgat tactggtgta aaagctgatg aaaatggttc taaaggtcgt
    25681 cgaatgaatg ggattttgaa cttgatcaat tcagcaaata aggcagaaac agccactgcg
    25741 ggtgcagtaa caagaaaaga tatcgatact ttatttaaat tgatgtatga aaaaggttat
    25801 atgggagaaa aactatgctt gatttctcca gatatgcagg agttaatgac tgatgagtta
    25861 gatggaaaat caacaaaaat tgttcagttc ggggaaagag taacttttgg attgcaaatt
    25921 ggaaatatcg tgtctaatta cggtacaggt attgctctac tagaaccatc attgccaaaa
    25981 ggaacaattg ccgcaatcga tactaattat gtgaaactac gtccattacg tgaatggaga
    26041 gcagaagaac ttgcaaaaac aacggattct agacgtatcg gtcttgtagg tgaatactct
    26101 cttgaataca atgcttcaaa ctctggggca attttaaatt taaagtctga ataaaaggga
    26161 gttagtactc ccttttttga taggaggaat tacagtggta aaaaaagatg aaactaaaaa
    26221 agatgaagtc gtgaaatata gagtaggtaa aactaaaaat tttgttgggt ttgttcatcc
    26281 taaaactcgt agatttatca cagcagattc aaataacgaa tttatcattt ctatagatga
    26341 taaagaagca attgcaattt tggaagatgc aattgatgtt aatgaaattt aggaagtgat
    26401 ctgatggatg aatcgctaaa aacggaaatc attgagtcta caaaagaaga ttttccagat
    26461 ttgagtgaag aacgcataac taatttatta gaaataattt tgctagaaat tgaatcatac
    26521 aacacttgta aaaatgatat ttcatgggaa aagttaaaaa gcgtgattag tgaagtgttg
    26581 tataaaataa taaaaaatga atcagaaaaa acagtatctt cagttagacg tggcgataca
    26641 acgattagtt atgcttcaac aacaaatgat gttagtgagt tacttctagg ctatggtgat
    26701 ttgatacgaa gagttattgg ttgtggagga ttggagtttt tttaatgaat gaagcagata
    26761 ttttagaaac tacttatgaa gatagttgta ttattgaaag actgacggac attgaagatt
    26821 ctaatacaaa tattactatt caagattaca aaaaagtata tgataatcct atttcttgcg
    26881 ctctttcaca aggtcaaatc gatggactag cagtcataga agatggagag atggtaaatg
    26941 tttcaactga cacatataaa ttatttgttc atcctaagat taaactaaaa aaaggagatc
    27001 gaataacaat aactcaaaag gccagtggct taattttttc tctatttgct actaagcctt
    27061 tttactatcc tagtcattgt gaagtgaatt tgataggaag tgagaaaaat gggtaatctc
    27121 aaatatgaat caaatgcaga aaagatcatt gagaatttta aaaatatgac tgtaattgct
    27181 caaaaagaag gaatatcctt cgttaacgat tcaatgaata aagtcgttag tttagctaaa
    27241 ccattaactc ctgtaaaatc aggtaattta aggcgaggat atcgtgtagt aaaagctaga
    27301 aaactatcaa gcggtcgaat tgttggagca gttataaaca atgaacatta ttttaaatat
    27361 gtagaagaag gtcataggac taaaaatggt ggctttgtca aagggaaatt catgttaact
    27421 cgtgcaacga atcttgcaaa tatgtcttat attcctcgaa gatttaaaca aatggcaata
    27481 aaaatcgtta agaaaggaaa gtaacatgaa agatgaaatc attgctgcaa tcagtagcaa
    27541 attaaaagaa atctatcctg atgggacaat atatcttgat tcagttatgc agtcaactaa
    27601 agatttttat ttcgttttat cagtaatgga atctggaact gaaaatgtag gaattgatat
    27661 tcaaaatgtt tctttcttaa ttgatattgc attgattgat aataaaccta atagaaattt
    27721 aattaatgaa ttagtctcac gttgtgggac tttttttaat acgattacaa tcgatgagca
    27781 aatattattt ccaaaagcct atttacctga tgaagcagat ggtgttcaac atattcgttt
    27841 tacattagaa tttccacaat atattgaatg gagtgaaaga taaatgggag aaaaaagaag
    27901 taaaaccgga attatttctg tcgaaaaacc tacttggttt cctttggaag atgagacagg
    27961 gaattttccg gtatatggta ctgcgttgcc aatgggaaca gcagtaagta tcaaaccaac
    28021 agctaattat gaaacaacac aagattatgg ggattcagtg gttcaagatc aatttacagc
    28081 ttttggtggt gcagaagttg aactggaagc aaatggatat actcataaag ttttatctac
    28141 aattactgga gggaaaattg ttaaaggtgg tgccttgcgt tctggagaag atattgcgca
    28201 agatggtgcg tttgcatata gacgtaaaaa atcgaatggg aaataccgtt atacggtctt
    28261 ttataaagga cagtttgctc tagattcaga tgaaacatca acgattgaag gaagtaaagt
    28321 aagttttact catccaactt ggaagggttc atttgtagat gtaccaggac ttggatatat
    28381 gtattcagtt gatgaagatg atgaaggcgt ggataaagca atgatcggaa attggtttac
    28441 aaaagtagca attccaattg aagaagcaga actttcagga ggtacagaat aatgtcaaaa
    28501 tatcaaacaa cgattaaatt aacgaaaaaa gatgaagaag gtaaatatga gcaagtacaa
    28561 tttaaatctg ctgaatttct accaggccca gtcgtagaag aagcagcaga agtaatggag
    28621 ggaatgcaaa atgctgtaga tggaaaatca gtatcagaag cattaagtcg agcttattca
    28681 tttattgcag atattttgtt tgaaggacag tttactggtg aagattatcg tcaagggatc
    28741 gatgctcgag aaattgcttc gttgacaggt aagttattaa aatctgttac tgcaggcttt
    28801 gatgaaactt atacagaaac gaaaaaaaag taagcgaagc tctcaaatat ccttttttta
    28861 aacattcgat tacttatcga gaattagatt taaagacgca attactcgaa gcaggttgga
    28921 cgttaccaga aattgaacgt actgactttg acgagttaat gcgtcttttt gcttttcgag
    28981 atgcagtaaa agagcatgag gatgttgagt actacgataa cttcacccaa ttttaggagg
    29041 tgatactttg aataacgatg acctaattct gaagatgata ctagatgaat ctggttttac
    29101 tgccggtatg aataatgctg tcaaaaagtt gaactctttt gatgaaacaa tcgaaagaaa
    29161 aagtagaaat agtggcagtt cattaggtag tatttggaaa atatttgctg gaagttttct
    29221 agctagcgga gtaactagaa tcgtaggagc tggctttgat ctaatcaagg gttccataag
    29281 tggagcggtt gatcgagtag atacgatgaa taacgctcta cgaaatttcc aaaatatggg
    29341 atttagtaat tcagaaatta tgaagaatat cggaaagaat gggcttttat ctcaaggtat
    29401 tcaaggactt cctactgctt tgaatgatgc gataagtcat gttcaacttc ttgcttcttc
    29461 tacaggtgat ttaactcgtt caactcaaat atttaaagct ttaaatgatg gaattcttgg
    29521 tttcggtggt tcaactgatc aagttaatga agctgttatt caattatctc aaagtttctc
    29581 aaacggaaaa gtagacgcac aaacttggaa ctcaatgatc aacgctcaac ttggacctac
    29641 tctttctgct attgctaaaa agatgggaat tacaatggga gatctgaaag aaggtttatc
    29701 tcaaggtaag atttctgttg aagaattcca aaatcaatta atagaaatgg ataccaaagg
    29761 tggcggagga cttaaatcat taagtcaaat cgctaaagat tctactaagg gaattaaaac
    29821 ctctatacaa aatgcaaaaa cagctgttac acgtggtgtt ggtgaggtaa tagaaggatt
    29881 aaataaagca ttagtagatt cagatttagg tggatttaag ggaattattg ataaagtggg
    29941 cagttcaatg gaatcgttct taaaagtaat tgctgcaaat attcctacag cagtatcatt
    30001 tttaagtaat ttatttaacg aaattcaaaa atttggttct gctttgaaat tcatgatgcc
    30061 ctttcttgtt cctgcagcta ccgcttttgg agcgttcatg tttcaactta aaggtatacc
    30121 agcaataata aaaagcttca ataattttaa gaatgccata attggcgttg gaaattcatt
    30181 aaagattatg ggggcaatag ctgctgctaa ttcatttgtt ttaattattg gtgcagtcgt
    30241 tggtgcaata gctgttttcg gttactttat ggcaaccaat gaagagctta gaaacaaggt
    30301 tatatctatt tggaatgatg tgaaagattc cgtaattggt gtattaaaaa atataaagga
    30361 ctggggaatt gatacttgga attctgctaa ataaatggca tcaaatgcag ttgagggtgt
    30421 caaagatgct tggtcaggaa caaaggaatg gttttctaac acctggaaag gtattaagga
    30481 tggggccaca ggtttatttg ataaaacagt agagacttca agaaatgcag tcgatagtgt
    30541 aaaaaatgca tggtctaata tgaagaaatg gttttctgat acttggcaaa gtataaaaga
    30601 ttcagccaga gaaaaatgga atggaatcaa aggttctatt atgtcagttg ctggtccatt
    30661 gattacaggt ataaagaatg catttttaca tgttactttt tatctagaga ccttatggaa
    30721 taatttagtt gagattggta aaaacgtctt tgagatttta aaaaatgtta tacttgcgcc
    30781 ggttttgttt attacctcca tgatttctgg tggatgggaa gaaacaaaaa ataatatgat
    30841 tgctgtttgg aataatatta aagaaagtgc cataaatatt tgggaatcta taaagaatat
    30901 atttgtaagt tattttacaa atatttattt tgctgcactc aatatttgga cagggttcaa
    30961 gcttacattg ataaatattt ggaatgaagt ggtaaatcaa gctaaatcga tttggttaaa
    31021 cataaagtat
//
LOCUS       DAZBSA010000021        30923 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000021 DAZBSA010000000
VERSION     DAZBSA010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.021"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(296..1024)
                     /locus_tag="ACHGMT_000907"
     CDS             complement(296..1024)
                     /locus_tag="ACHGMT_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6834674.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="ACHGMT_000908"
     CDS             complement(1002..2576)
                     /locus_tag="ACHGMT_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFN6834675.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="ACHGMT_000909"
     CDS             2805..3743
                     /locus_tag="ACHGMT_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6834676.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="ACHGMT_000910"
     CDS             3755..4666
                     /locus_tag="ACHGMT_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6834677.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="ACHGMT_000911"
     CDS             4690..6162
                     /locus_tag="ACHGMT_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFN6834678.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="ACHGMT_000912"
     CDS             6213..6824
                     /locus_tag="ACHGMT_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFN6834679.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="ACHGMT_000913"
     CDS             6854..8767
                     /locus_tag="ACHGMT_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFN6834680.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="ACHGMT_000914"
     CDS             8895..11876
                     /locus_tag="ACHGMT_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFN6834681.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="ACHGMT_000915"
     CDS             11873..13534
                     /locus_tag="ACHGMT_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFN6834682.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="ACHGMT_000916"
     CDS             13574..14506
                     /locus_tag="ACHGMT_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFN6834683.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
     gene            complement(14898..16445)
                     /locus_tag="ACHGMT_000917"
     CDS             complement(14898..16445)
                     /locus_tag="ACHGMT_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFN6834684.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(16547..16900)
                     /gene="tnpB"
                     /locus_tag="ACHGMT_000918"
     CDS             complement(16547..16900)
                     /gene="tnpB"
                     /locus_tag="ACHGMT_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFN6834685.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(16890..17084)
                     /locus_tag="ACHGMT_000919"
     CDS             complement(16890..17084)
                     /locus_tag="ACHGMT_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834686.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            complement(17310..18512)
                     /locus_tag="ACHGMT_000920"
     CDS             complement(17310..18512)
                     /locus_tag="ACHGMT_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6834687.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(18515..18709)
                     /locus_tag="ACHGMT_000921"
     CDS             complement(18515..18709)
                     /locus_tag="ACHGMT_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFN6834688.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(18756..19604)
                     /locus_tag="ACHGMT_000922"
     CDS             complement(18756..19604)
                     /locus_tag="ACHGMT_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFN6834689.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(19809..20159)
                     /locus_tag="ACHGMT_000923"
     CDS             complement(19809..20159)
                     /locus_tag="ACHGMT_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834690.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            20337..21200
                     /locus_tag="ACHGMT_000924"
     CDS             20337..21200
                     /locus_tag="ACHGMT_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN6834691.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
     gene            21824..22816
                     /locus_tag="ACHGMT_000925"
     CDS             21824..22816
                     /locus_tag="ACHGMT_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6834692.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            22832..23716
                     /locus_tag="ACHGMT_000926"
     CDS             22832..23716
                     /locus_tag="ACHGMT_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6834693.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            23765..25372
                     /locus_tag="ACHGMT_000927"
     CDS             23765..25372
                     /locus_tag="ACHGMT_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN6834694.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            25373..26857
                     /locus_tag="ACHGMT_000928"
     CDS             25373..26857
                     /locus_tag="ACHGMT_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFN6834695.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            27034..28026
                     /locus_tag="ACHGMT_000929"
     CDS             27034..28026
                     /locus_tag="ACHGMT_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN6834696.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            28078..28947
                     /locus_tag="ACHGMT_000930"
     CDS             28078..28947
                     /locus_tag="ACHGMT_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFN6834697.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(29367..30896)
                     /gene="guaA"
                     /locus_tag="ACHGMT_000931"
     CDS             complement(29367..30896)
                     /gene="guaA"
                     /locus_tag="ACHGMT_000931"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFN6834698.1"
                     /translation="MIVLDYGSQYNQLITRRIREFGVFSELMSHRITAEEVKAMNPKG
                     IILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGKA
                     ELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKMY
                     GIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLGL
                     SGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAKE
                     RFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGTE
                     TAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPGL
                     GIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRTY
                     DYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVEW
                     E"
BASE COUNT         9693 a         5151 c         6241 g         9838 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagttgaaa
    14821 agtaagcgcc ccatagaaca gtacctgtac gtcaaataac ccgacctata gaatagaatc
    14881 gggaatgttt ggcgttttta gttacgagaa aaatgttgtt gtacatcctt gttccaaggc
    14941 aacagttgat ctaaagtttc tgctgaaagt acgggaagat ttggtatctt atcaaataga
    15001 aactctagat actttacagg attcaatccg ttaagttctg ctgttttata tacactcaga
    15061 atgattcctg aagttcgtgc tccttcaaga cttgtggaat gcaaccaatt tttccgtcct
    15121 atcaccagct ctttgatatt tcgctccgca cgattatttg tgagttctaa acgaccatct
    15181 tcaaaaatag gttcaaaacc acttcgtagt ttacacgcat attgaacggc tgcatccaac
    15241 ttggattttg caacaggatc tatcgttgcc atccactcat aaaaagcaat aaataatgga
    15301 cgcagttctt gttgccgaca gcttaatcgc ttctcaggag aaaagtcttt ccatttcttt
    15361 tctaggacaa accatttatc caattgtttg actgcttgag ctgcgggaat atccgtgttt
    15421 ttctttcctt ttggaattgc ttcgaaaaat ttccgccgaa catgcgcgaa acaatagaca
    15481 agtcgtacac tttccacggc attatacccg ctatagccgt cacagtgtaa ataccctgta
    15541 aactccttga gaaaagtttt cggaacatct ccagcacgac tttcgtcgtg gtgataataa
    15601 acgatgggat gctgactgtc ttttcctgta caaaactgcc agtaataggt atctgttttg
    15661 gcactttcaa tcacacgata acttgtttca tccccgtgta gaatctcttc ttgattaaga
    15721 tagactctta atcgttctcc taaagggtct aacgcgtttt gcactgcttt aatatgccaa
    15781 ttggtgatat tatctctcga aatatccaac ccatgtgttt gtttccaata gttctcttgc
    15841 cgataggcag gaactttctg accaaatttc atccggatcg tttccgccac taaactattg
    15901 gaacctaagc tattagaaat caaaggttta ggaacgatcg cttttttaaa agaagaaagt
    15961 ccttcttttt cgcagctttt acaacaatag gtatgttgat aatgggtgag acaatccaac
    16021 atagcctgat ggaaacgaat ttcttctcgt acttttgtcc gtccgatatc tttcatctgt
    16081 gaaccgcaat gttcacaaaa ttgttcttcc aaaggaagga gatgttcttt ctctgttata
    16141 ggtaagtgtt tgattttgtc tgctttcgtt cctacaactc gttgatgtcc ttttactgga
    16201 ataatggtgg cagctgcttc tggaacactt acagaaattt cttcatcact aaaaagattc
    16261 aattgcccat tcgtttcctc aaagagagtc tctttagagc gtccaaataa tttgttggtt
    16321 aaataattga ccttttcatt caatagttgg atttcagttg taagattctg aatggtttgt
    16381 tgttgtgctt gattggtttt atgggcttct tgaagcaact gaagcaacgt atctgtagtt
    16441 tccaaaattt ttcaccacct tgaatcgaac tgattttgtt acagaatacc acaagaaacg
    16501 cacaagaaat aggggttcaa cacatttttt gtgttaaccc cccagcttaa aaaactcctt
    16561 tttttcctgg caaaatagct cgaggttgat cgatacttaa tccttctaac aaccatcgaa
    16621 cctgttggtt cgttaaggtt cgaacttcgt ctttcgtccg aggccatttt aacttgccgt
    16681 tttctatccg cttataacag agaagaaaac catcaccgtc ccaatagaga agtttgaaac
    16741 ggtcttgacg gttcccgcaa aataaaaaaa cggcatcttc gtaaagctcg agttgatact
    16801 cttgttgaat aagactggca agaccatcaa ttccttttcg caaatctgtt tttccgcaca
    16861 caatataaat atgtttggtt tgaagtaaat cataaagcat tggacaacag ttctttcaaa
    16921 atagcttgaa gaatataatt gttaataccg ttatagatca aaacttcaga accattctct
    16981 cttttgatgc gcatcgctaa cttggcagcc gcgggtttac ctttttctgg tgcactcttt
    17041 tcgtttccta attggacagg aacaatctca tttaatttat acatagaaaa cctccagact
    17101 tcttgatagc tcaagtatat cttgaggtgc atttgttagt aaggtactgt tctatggggc
    17161 gcttacgttg aaaaacgttg atttatcggc attccccatc catattatat ttacgtctta
    17221 ttcccactca atatgagtag cctcgtaaag aagctattat atcagtgctt tttcagccta
    17281 ttcctcattt tggtagggaa atggtagggt tagattttta ctgcttgatt tccattaaag
    17341 tttgcaactt tagattttgt tgcacaggta aatttaatgt ttcctgtcat ttgttctgca
    17401 attccttcat cattacggct ccatagatga gcgtagtgct ttaaagtgat ttcagggcta
    17461 gaatggccta accgttgcga cacgaccaga atgtctgcgt taaactcatt gattaggtag
    17521 ctaacatgag aatgacgtaa tccttttcct tgtattaggg gtaccttagc tagtttggca
    17581 taacgctgaa taattcgttg aacagttgaa cgatagagtg ggaggtcagt gtagctaaga
    17641 ataaattgtt ctacaccatg atttttttga ataactttcc acttttttag cacttcaatt
    17701 gtatcatcgt ccagagaaat gatccgcaag ccgctttcag tttttgtgta aggtttccga
    17761 ataaaatctt tttgatttgt catttccaag gtgtgatgaa ccctcaactt tttacgtttg
    17821 aagtccacgt cattccaatt aagtgctaat ccttcgctta cacgaacacc agtcatgtaa
    17881 tatagccaga tcattaaaaa acacatatgt tcgtagaaat catctttata aatttttgcc
    17941 aaaacttctt caaattcttc ttttgtccag taaggaacga ttgattttcc ttttggaatt
    18001 gcttttgttc ttttggaaat attctcattc agaaattgca gggtgaccgc atagtctaat
    18061 gatttacgaa acataccgta cataagtgaa ctgtatgctt gtgagtatcc gctatcgttc
    18121 aataaccaaa ttcgataatt ttcacaatct cgcacattga tttcacgaag cttcttatca
    18181 ccgaaaaatt cagtaatctg aatcaatcca cactcacgag aactccaagt acttttttgt
    18241 acgctagctt tgtagtgtgg aacgtaagac tgctccataa attctttgta agttaaatca
    18301 taattcgcaa atccatttac aaccatgtat tcatttttta tacgcatgac ttctttgtgt
    18361 gcttcacgag ctgatgagaa tcgttttcct agagaatctt ttgttccttt cttctggatc
    18421 ctctttccag tcacttgatc tgttcctaac tcaactagat aagaaaagcg accattttta
    18481 tcttgataca cacaagggta ttttgttttc atagttattc ctcctgtgaa agaggaacac
    18541 ctataatttc ttcaattact tgtcggggta ctaccccaac tcttttatta ttataatatg
    18601 gatatccctg atttaccatt cttgtttttg cttgtcggat tattgtggta gcttgatgtg
    18661 ttttaaatcc tagagcctct aaatcttttc gacaaattgt atttttcatt gtataaactc
    18721 actttcaaag aaaacactat cattgggtaa tagtgttatt tctttatttt tgtataatga
    18781 acggtacttt cttgaaaatc catcacttgt tcaggacttc ccaactcatt taaagctaaa
    18841 gcattgtaaa aaaatggacg tctaactata tattcgcttg tatatagtat atcttgattc
    18901 gtggaatcat agggtttctt taccatcttt ttggtgacag ttggtagttt gagattccgg
    18961 gacttgaaaa aagctttttt cttatgctct ttcaattcca gctctttatc gaaatatttg
    19021 ctgatatagc gcccgcgatt ttcgatactg tctacatcga tcttgttgat cttaacgaaa
    19081 ccctgacccc aaatcgtagt taaccggtta aatgagataa aggggaaatc aaacaagata
    19141 atatggtagt ggattgcacc gcgttgctgc ttttcccacg tggcgagata ctttaaattt
    19201 gcttttttag ttttatagag gtcataattc agtcgtttga taaatttttt aaattcatta
    19261 tttgcatact caatgtcttg aatgttttct ttgaaagtga gagttaaaaa tttcgtttga
    19321 ttgtcaaaat tcgtgtctac aaggcgggca atctcaaatc gcatactttg ataatgtttc
    19381 tgtttgcgtt ttaaactgtc atattgtcct tgagcggata attcatcata gttttttctt
    19441 ttttcaagag taatatcatt ttcataaatc caagctaatt tttgttttcg ttccagattt
    19501 acttcgtttt cgatcgtttt acgtttggga gtaatgattg cttcataatc ataaatttca
    19561 atatacgtgg gagtttcaat gattttctga ttataagctt gcaaaaatca tcactccttg
    19621 ttgcgtattc gattgtcaaa gaaccagtca ttttctcctg atgttgatct ataaatcaag
    19681 ttaaggggga aggcctgcgt agcagccctt ctagcctatg gggtgttggg cggtcttaac
    19741 ggacagaaac ggctctgtgg gcgctgcccc gccgtttcgt cctaaccacc caacactcca
    19801 tagtatagtt agttttgtgt agtggtacct tgttttctgg gaatcaaagt tgccgtatca
    19861 gcggttgccg ataacccata actgacaaat tgtccattcg agcgtcccca aggtacaatc
    19921 accaaattgg taaatgtaat tatggctaaa ccttgttggg taagttcttc cggcgtgatc
    19981 acaggatgtt cagatttgac tttgacactg attttttcgt agcgaagttt tggtaggata
    20041 cattcgtagc tccaacctag aatttcgccc tccttttgcc agcgagtgac attgacgact
    20101 tggtattctt tttcgagtgt ttcttttggt aaactgagat cggtaatttt tattggcatc
    20161 acaattctcc ttttttatac gaaagtttat acgatttgat taaaaaatat agatggccat
    20221 ctacgacttg tattatatac gagaacttat acgtagtcaa gagattttct aaaaaaatat
    20281 atacgatttt ttatacgagt gttataatga aaaaaatgat aaggaggaat ttgaatatgt
    20341 ttggtttagt acaacctaat aaagtgaagg tgggccaacg aatcaaagaa ataaaagacg
    20401 gaatgtctct ctcttttaca gaattaggga atcgattagg tttaaaaaag ccgacgatca
    20461 gttcctacgt tcaaggctat gcgttggctc ctaagagtgt gatcaaccaa ttatctagta
    20521 tcagcggaaa acctgtcggc tggttttatt ttgggcatgt ggaagaatat atccgagagt
    20581 atttgtttct cattggtcag aaaaaaatag tggacgatta tcctgaaatc attgagaaaa
    20641 taaagcaaga attttatacg ggcgagttca aaaatcctgc ttgggagaat gagctaggct
    20701 atcctttgga ggaatttatc caagaatgtt ttgtagaata ttcgagtgaa atcatgaagg
    20761 actatgtcgt acagattacg aaagaggtac ttgaagaatc caaaacgcta aacaaactat
    20821 catcaaaagc aaaagaagaa gctgtcacga ttctttcatc tgggatcatt gactatatcg
    20881 agatgagtgg tgagatcaag tatggagaga aagatcgcat tgcgaaaatg gtgaaagaat
    20941 caataaaaac ctttgatgtg aaacaagacc tgacatttaa tgattcctat ttgattggaa
    21001 agttgatcaa tattcttggt acagatgaag atacggagct gtttctttct tctttatcaa
    21061 tgatatttac tgataagcat ttttccactc gatttggtgg ggaagaatta gtcgaaatat
    21121 ttcaatcgat gcgaccagcg ttaatcaaac tctatcggga gaaaacacca gatgaatttt
    21181 atgattggtt tgagaagtaa aataagaaaa gtggcttaga ttacaagttg gtgaaaaaat
    21241 ttttttcagc ataacatcag taatctataa aggggttgtt gattatactg tagaagttaa
    21301 gtactcaaga gattttttgt acgtaaacaa agcaaaaaag ggtgcagttt cactagaaaa
    21361 tgtactcttt tttgtgattt ttcttagtta tagagttttt ttgaaacttt aaaaatgaga
    21421 aaacatattg attttaaaga gtgtagacat agcttctttt gctaaataga ttaaacttac
    21481 tggtgagtgg tcttgaacat acttttaaat cacattttac tgagttttat tggattttat
    21541 aattcattgg aaacctcgta cttaatctgt ttttatctat cgactagaat ctgtatatat
    21601 tccagctatc aatgaggccg cagaaaaggg tgcttaaaaa taatctgttg tttaaaatat
    21661 tattatcaga atacttagat acaaaattct ggcacaaagt aaaaaaaata ttgacttaaa
    21721 aaaatggata tggtaatcta taaacgaaaa tagtggtaac gtttccaaaa tgcatcttcg
    21781 ttaaattttt atttttttaa acgaaaatgg aaacgtttct aaaatgtatt gggaggagat
    21841 tgaattggaa aagacattgg gaccaggtgg tgagataagg gcggtacaac gggataaaaa
    21901 aaatatttcg aagtgggaaa aattaaagaa acagttgcgg cgtgataagt ggatgtactt
    21961 gttgctctta ccaggtatca tcatgacgtt tatctttaag tatgtgccaa tgtacggtgc
    22021 tgtgattgca tttaaagact ataatcctat gctaggaatt ttggaaagtc cttgggtagg
    22081 actagatcac tttagggaat ttatcagttc accaaatttt acgcttttgc tgagcaacac
    22141 attaaaaatt agtatttatg gtttattgtt aggttttttt ccaccaatta ttttagcact
    22201 gagcttgaat caaattatca ataaaaaaat aaaacagaaa atccagttga ttctatatgc
    22261 gccgaatttt atctcagtag ttattattgt ggggatgctg tttttgttcc tatccgcaaa
    22321 tggacctatt aatgggttag tagaaaaggt tacggggcaa ccaattatgt tcatgtctaa
    22381 tccagcttat ttcagaacga tttatatttt atcgggaatt tggcaaggaa tcggctggtc
    22441 atccattctt tacaccgcaa cgctggcggg agtcagtacc gatttatatg aggcagcaaa
    22501 tatcgatggg gcaaatattt tacaaaagat ttggcatatc gatttgcctg cattgaggcc
    22561 tgtgatggtg atcagcttca tcttggccgc cggcggtatt atgaatgtag gatatgaaaa
    22621 agcctacttg atgcaaactt caatgaacat ccctgcctca gaaatcattt ccacttatgt
    22681 atacaaagtc ggcttgcaat caggtgatta tgcctactct gctgctgttg gtctgttcaa
    22741 tatgattatt aatgtcgttt tattactgat tgtcaacaat ataaccaagc ggctaaacga
    22801 tggtcaaggc ttgtaatgaa aggagaaaat tatgaatagt gctttatcaa aaatcgaccg
    22861 tcaaatctta tggttgaatc gagtgattgt aactttttta gtcgtcatca ccattgttcc
    22921 gctgatttat gtactggtag cttcttttat ggacccagct actttgatca ataaaggtat
    22981 aagcttcaat ccgaaagatt ggacaataca gggatatcaa cgggtatttg cagatgattc
    23041 cattttacgc ggttttgtaa attcgttgtt ttactctttt agcttcagct tattgactgt
    23101 atttattacg atgatcactg cgtatccttt gtctcgacct aacttggcgg ggaaaaagat
    23161 tattatgact ttttttgtca ttacaatgtt cgtgggtggt ggcttgatac ctacctattt
    23221 attggtaaag aacttgggca tgttagatag tgtatgggcg attatcgtac caggtgctat
    23281 aaatgtgtgg aatattatct tagcccgtac ctattttagt gctttgccaa aagaactttc
    23341 tgaagctgct actatcgatg gcgccaatga cttgcaaata ctgttcaaag tcatgcttcc
    23401 attagcgaaa ccaattatgt tcgtattgtt tttatatgcc tttgttgggc aatggaactc
    23461 atactttgat gccatgattt acatcaaaga tcccgactta cagccgttgc aactcgtatt
    23521 aagaaatatt ttgatccaaa accaaccttc acaagacatg gtgggagcca atactgccat
    23581 ggcagaaatg aaacaaattg ctgagttgat caaatacgca acgatcgtca tttctagtct
    23641 gccattaatc ataatgtatc ctttcttcca aaaatatttt gataaaggtg ttttagttgg
    23701 ttctgttaaa gggtaagtat tgttcactat ttgtatgaat aaaaattata aaggagattt
    23761 taagatgaaa gcaaaatgga tgctgccaat cgtttttact tccgtactaa ttttgggggc
    23821 ttgtggaggc tcaaaagaaa agagtaaatc aagccctgat tatgaactag aaaatgtctc
    23881 ttttccattg aaagaaaaag tatctttaaa aatgatgtct caaagtgccc ctttggctcc
    23941 caatgatcct aatgacaaat tgattttcca acgattagaa gaagagacca atctccatat
    24001 tgattggaca aactacagta gtgattttgc cgaaaaaaga aatttggata tcgctagtgg
    24061 tgatttacca gatgccatct tcaacgccgc tgcaggtgac tatgatttat taaactgggc
    24121 ggaaagtggc gttattgtac cgattgaaga tttgatcgaa gattatatgc caaatctgaa
    24181 gaaaattttt gatgaaaacc ccgaataccg acaaatgtct actgcacctg atggtcacat
    24241 ttactctatg ccttggattg aagaattagg cgaaggaaaa gagtcgattc atacagtaaa
    24301 tgggatggct tggatcaaca aggagtggtt ggataatttg ggtcttgaaa tgccgcaaac
    24361 cactgatcag ttgatgactg tgttggaggc tttcaagacg caagatccaa atggtaatgg
    24421 tgaagcagat gaaattcctt ttagctttat taacaatgga ggaaacgaag acttaaaatt
    24481 tttaatgggg gcattcggtc taggtgataa tgatgaccac ttagtcgttg ataacgatgg
    24541 tcaggtacaa tttactgcta acaaagaagg gtataaagaa gccatcaaat atttcaatga
    24601 aatgaataat aaaggtctta ttgataaaga ggctttcgaa caagattgga atacgtatgt
    24661 tgccaaaggg aaagaacata agtatggcgt gtactttact tgggataaag caaatatcac
    24721 gggtgcagat gatagttatg acgtcttgcc agtcttagcg ggaccagatg gaacgaaaca
    24781 tgttactcgt acgaatggca tgggtttctc aagagatcgt ttcgtaatta ctagtgcgaa
    24841 taaaaacttg gaacttacag ctaggtggat agataaaatg tatgaaccgt tacagtctgt
    24901 ccaaaataac tggggaacct acggtgatga agcacaggcg aatatttttg aattaaagga
    24961 tggtatgtta aaacatttgc ctttagaggg gacagcaccg ggggaactaa gacaaaaaac
    25021 cgaagctgga ggaccattag cagttcttga ttcttattac ggcacagtca ctactatgcc
    25081 agatgatgcg aagtggcgtt tagatttgat gcatgacacg tatttgccat atattacaga
    25141 agacaatatc tatccacgtg tctttttaga aaaagaagaa acagatcgat tagcaaaaat
    25201 cgaaacggat atgtgggatt acatctatcg caaaagagca gagtggatca ccggcggtaa
    25261 aatcgatgaa gaatgggaag aataccttgc tgaattggaa cgtgtaggtt tggaagaatg
    25321 gattcaaata aaacaaactg gctatgataa ttttttgaag ggagcacaat aagtgttagt
    25381 agaagaaacg gcgattttgg ataaagcaaa tcgatttatt gaaatgatgc gaggaaaagt
    25441 taaccctcag tatcgacaaa actatcattt ttccgcaccc atcgggtgga taaatgaccc
    25501 caatgggttt atatattaca aagggcatta ccatctcttc tatcaatact atccttacga
    25561 tagtcgatgg gggcccatgc attgggggca tgcgcgcagt agtgatttgg tccattggga
    25621 agatctacca gtagcgttag ccccagatca accttatgac agcggcgggt gtttctcagg
    25681 aagtgcaatt gaaagagacg gaaaactata tttattttat acaggctgtc aagaaattga
    25741 tggtattagc catcagcatc aatgtttggc ggtctctgaa gatggtattc attttagcaa
    25801 atacagtggt aatcctgtgg tagcagaaaa acagctaggt gaaaatgggc taatacaaga
    25861 atttcgtgat cctaaagtaa ttgttcacaa cgatggttac tatatggtag tagctactaa
    25921 atctacacag tctactggta gaatagtgat gtttcaatcg tcagatttgt atacttggca
    25981 cttttactct gtgctgctag aagggacttc agagcaagga ataatgtggg aatgtcctga
    26041 tttattccat ttagacggac gagatgtctt aatcatgtct ccgatacaaa tcaagaagaa
    26101 cggactggaa tatcataata tcagttctac catggcttgt attggtgagg taaactggga
    26161 aacaggtcag atgatagttg ataatttcca tgaaatggat tttggaatgg atttttatgc
    26221 accacaaaca acaagagatc ctaatggtgg acgggtgatg attgcttgga tgcagatgtg
    26281 ggatcgtaca ttgcctacac atgagttagg ccatctttgg gcaggctcta tgagtttgcc
    26341 ccgagagtta agagtgaaaa aaaatcgatt ggtacaaaaa cccatctcaa ctgtatatag
    26401 tcatttagtt tatcaacaag gcagtgaaaa tattgcagtg actgacacat cagtcgtatt
    26461 tcgaaacgcg ctgtctgatt cttcatattt acatctagtc gcagatttgg agaaagctga
    26521 agtttttaca attgaattac tgcggtctaa gacggattgt ctcaaactag cctatgacac
    26581 tgctacacag gtttttgaat tggatcgtca gaaactagga agagagctta caggggcgga
    26641 agaagaccct ctgaatgtac gaaaagttga agtacctttg attgataatc agctcgttct
    26701 tgagattttt agagatacct catcaattga agtctttatt aatgggttag aagttatgac
    26761 ggctactttc tatgagctgg aaaaagctcg agatatcttg tttcattcga tcggagaaag
    26821 tcgtattcaa gcttttgaaa cagcgagtgt gaaataaatg tttgattgct taggcgatga
    26881 atcaattatt taataggttt tgaaggataa tgtttttaga aaagaacaga tagctagatt
    26941 tcccatggat ttgctattag tattttgaaa aaattagtaa tttatgacaa aattcattat
    27001 actaaacttg tgcagaaatg tgagggatca actatgaaag caacaatgaa agatgtcgca
    27061 aaattagcag gtgttggagt aggaactgtt tcccgggtat tgaataacgg ttctgttaaa
    27121 gcttctacta gagaaaaagt tgaagcagca atgacagagt taaactatca accagatttt
    27181 tatgcccggg ggttaaagat gaagcatacg aacacggttg ctttgattct acccacggta
    27241 tggcatccat tttttggcga atttgcctac catgtagaaa cagcattaga aagacaaaac
    27301 tacaagcttt atttgtgtaa ctccagtggt aatagtgaaa aagaacaaga atatatccag
    27361 atggtcagcc aaaataaagt agacggcatc attggaatta cttattcaga tatcgatgaa
    27421 tacatttcga gtaatctgcc ttttgttagt attgaccgtt atttcactga agaggtgatt
    27481 tatgtgaccg ctgacaatgc ggccggtgga cgtttggctg caaatgaact ttatcgccga
    27541 ggatgtcaga atgtagcata tatcggtggg taccaagaaa caccgaatga aacgaagagt
    27601 cgtcgaaaat attttgaaat cgagtgccgt caactgggga tttcctataa agcgttagat
    27661 atgttagaac ctttagacca tctctcagaa cgtgtaacaa attttttgtt agagaatccc
    27721 gatatcgatg ggatttttac gatcaacgat ccaatggcat tagctgtcat tgatatgctt
    27781 gaaaaaatag ggaaaaacgt accagaggat gtccaagtta tcggatttga tggtcaaaag
    27841 atgacagaag gagatcctta tctagtctca acgattgccc aacctacaaa gcaaatggcg
    27901 gaagtggcag tggcaaaatt aattgctgtc attaatggag agacagtaga aaaacgtacc
    27961 gtattgccga ttgaatttgg cgaaggtgac acaacgaaac ctttagtgag aaacgatttt
    28021 gcttagtgca aatacgtttc tttttttgaa aatgagtgaa tgaaaggtgt agataaaatg
    28081 ttatatggtg gaatagaagc cggtggaaca aagtttgtct gtgggattgg taatgaacaa
    28141 ttacaagtga tagaacggaa aagttttcct accacaacac cagaagaaac gatggagcaa
    28201 gtgtttgatt tctttgataa gtacaaagga aatatcgcat caattggtgt aggagcattt
    28261 gggcctattg atattcaaat cgaatcaaga acttacggat atatcaccaa cacaccgaag
    28321 ttagcttggc aaaattatca ttttgtagga gctttggaaa acgccttgtc ggttcccatc
    28381 tattggacta ccgatgtaaa tgctgcttgt tatggagaat atatggcagg gtatggaaaa
    28441 ggtaaaaata gtgttctgta ttacaccgtt ggtacaggtg ttggtggtgg tgctgtagtg
    28501 gatgggaaaa tagtcgcagg ttttagtcat cctgaaatgg gacatatgct cttacaacgt
    28561 cataatgaag atcaatttga aggaatctgt ccttttcatc atgattgttt ggaagggtta
    28621 gcatcaggac ctgcgattga aaaacgcttt ggaagaaaag cccagactat ctcatttgat
    28681 catccttatt ggacgattga ggcagattat cttgctcagt gtgtgtataa cacaacgtta
    28741 ctatatgcac cggaagtaat aattttaggc gggggtgtaa tgaaacagcc gcaactactc
    28801 gagttgattc gccaatcgtt taaagaaaaa atgggttcct acgtggatac gcctactctc
    28861 gaagaatatc tactaactcc aaggcttgct gacaatgcgg gcttgattgg ctgcctagct
    28921 ttagcaataa agaaactcaa tcattagaat agtatttatt agaactattc ataacatacc
    28981 ggaagtttaa aagggtattc tgaaacttaa atagtctcaa ttcattcttc tttcttgaga
    29041 atctgagtag agacttttat tctgtttcaa attaatataa ttacgaaatg atattgagta
    29101 caagaaagtt ctttttacca gatcttttaa atcgaaaaat ctgatatata tatctatagt
    29161 actaaaaatc tgattagata aaaagctaat tcaataggaa aataaaggta gtgaataaaa
    29221 gcaaagccct tgttagtagt taatctaaca agggctttgg tagggaaaat ggtaggaaac
    29281 catcttattt tttacgaaaa tcaggaactt tagtaattga ataatgttga tttataggta
    29341 ttcctcatcc atattatgtt ggctctttat tcccactcca ctgttgcagg tggtttgctg
    29401 gtaatatcat acacgatacg gttaacgtgt gcgacttcat ttacgatacg gacactgatt
    29461 ttttgcaaga cttcccaagg gatacgggca aagtcagcgg tcatgccgtc aatagaagtg
    29521 acagcacgga tacctacagt gtagtcatac gtacggccat ctcccataac cccaacagag
    29581 cgaatgcctg gtaggacggt gaagtattgc cagatgtcgc gatctaaacc ggcattggcg
    29641 atttcttcgc gcaagatagc gtctgattca cggacgattt ctaatttttc ttctgtcaat
    29701 tcaccaagga cacgaatacc taagcctggt ccagggaatg gttggcgcca aacgatgctg
    29761 tcaggcatgc ctaattgggt acctaattca cgtacttcgt ctttgaacaa tgtgttcaat
    29821 ggctcgatca gcttgaattg catgtcttca ggtaatccac ctacattgtg atgtgatttg
    29881 atggtttggg cagtttcagt tcctgattcg atcacatcgg tataaagggt tccttgtgca
    29941 aggaaagaaa cgccttcttc accggcaagt ttggttgctt catcatcgaa gacatataca
    30001 aattcatttc caatgatttt acgtttttct tctggatcag acacgccagc aagttttgat
    30061 aagaaacgtt ctttggcatc gacttttata atgttcaagc caaattttcc gccaagactt
    30121 tccataactt gttctgcttc acctttacgc aacaatacgt gatcaacgaa gatacatgtt
    30181 aattgatcgc cgatcgcttt ttgtaaaaga acaccaacaa cagaggaatc tactccgcct
    30241 gaaaggccta ataaaacttt tttgtcaccg acttgttcac gaatttttgc gatctccatg
    30301 tcgatgaagt tatccattgt ccagtcacca gtacattggc agacatctag tgtaaaatgg
    30361 cgcaacaagt catttccata ttcggaatgg cgaacttctg catggaattg gattccgtac
    30421 attttgcgtt ctgcattttg gatcgatgcg atggggcaat ctttgctggt agcaacgaca
    30481 tcgaaatcag tcggtgcttg ggtaactagg tcaccatgac tcatccaaac ggtttgtttt
    30541 tcaggagtgt tttggaaaag ggccgcttct gcagaagtga tttccaattc tgcttttccg
    30601 tattcacggt ttttggctgg tttaactttt ccgcctaatt tataagtaat caactgcata
    30661 ccgtaacaga ttcctaaaac tgggatcccc aaatcaaaaa tcttttcatc aataccaaag
    30721 gccttctcat cataaacact gtttggtccg ccagaaagga tgattccttt agggttcatt
    30781 gcctttactt cttctgcggt aatgcggtgg ctcatcaact ctgagaaaac accgaactca
    30841 cgaatacggc gagtgatcag ttggttatac tgactaccgt aatcaagtac gataattttt
    30901 ggctcttcgt caaatcgtgt tgg
//
LOCUS       DAZBSA010000022        30607 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000022 DAZBSA010000000
VERSION     DAZBSA010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.022"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(11..1594)
                     /gene="abc-f"
                     /locus_tag="ACHGMT_000932"
     CDS             complement(11..1594)
                     /gene="abc-f"
                     /locus_tag="ACHGMT_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFN6834699.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIVLVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(1699..1827)
                     /locus_tag="ACHGMT_000933"
     CDS             complement(1699..1827)
                     /locus_tag="ACHGMT_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834700.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(2172..2315)
                     /locus_tag="ACHGMT_000934"
     CDS             complement(2172..2315)
                     /locus_tag="ACHGMT_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFN6834701.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(2538..3017)
                     /locus_tag="ACHGMT_000935"
     CDS             complement(2538..3017)
                     /locus_tag="ACHGMT_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFN6834702.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(3090..3374)
                     /locus_tag="ACHGMT_000936"
     CDS             complement(3090..3374)
                     /locus_tag="ACHGMT_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834703.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(3452..3646)
                     /gene="ssrS"
                     /locus_tag="ACHGMT_000937"
     ncRNA           complement(3452..3646)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHGMT_000937"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(3718..5004)
                     /locus_tag="ACHGMT_000938"
     CDS             complement(3718..5004)
                     /locus_tag="ACHGMT_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFN6834704.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            5142..5813
                     /locus_tag="ACHGMT_000939"
     CDS             5142..5813
                     /locus_tag="ACHGMT_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFN6834705.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            5779..6267
                     /locus_tag="ACHGMT_000940"
     CDS             5779..6267
                     /locus_tag="ACHGMT_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFN6834706.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            6280..7227
                     /gene="prmA"
                     /locus_tag="ACHGMT_000941"
     CDS             6280..7227
                     /gene="prmA"
                     /locus_tag="ACHGMT_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFN6834707.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            7228..7992
                     /locus_tag="ACHGMT_000942"
     CDS             7228..7992
                     /locus_tag="ACHGMT_000942"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFN6834708.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            8225..10438
                     /locus_tag="ACHGMT_000943"
     CDS             8225..10438
                     /locus_tag="ACHGMT_000943"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFN6834709.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            10449..10895
                     /gene="dtd"
                     /locus_tag="ACHGMT_000944"
     CDS             10449..10895
                     /gene="dtd"
                     /locus_tag="ACHGMT_000944"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFN6834710.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            11066..12325
                     /locus_tag="ACHGMT_000945"
     CDS             11066..12325
                     /locus_tag="ACHGMT_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFN6834711.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(12414..13361)
                     /locus_tag="ACHGMT_000946"
     CDS             complement(12414..13361)
                     /locus_tag="ACHGMT_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFN6834712.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            13755..15047
                     /gene="hisS"
                     /locus_tag="ACHGMT_000947"
     CDS             13755..15047
                     /gene="hisS"
                     /locus_tag="ACHGMT_000947"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFN6834713.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            15062..16831
                     /gene="aspS"
                     /locus_tag="ACHGMT_000948"
     CDS             15062..16831
                     /gene="aspS"
                     /locus_tag="ACHGMT_000948"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFN6834714.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            16940..17815
                     /locus_tag="ACHGMT_000949"
     CDS             16940..17815
                     /locus_tag="ACHGMT_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN6834715.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            18081..19964
                     /locus_tag="ACHGMT_000950"
     CDS             18081..19964
                     /locus_tag="ACHGMT_000950"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFN6834716.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(20039..20323)
                     /locus_tag="ACHGMT_000951"
     CDS             complement(20039..20323)
                     /locus_tag="ACHGMT_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFN6834717.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(20313..21047)
                     /locus_tag="ACHGMT_000952"
     CDS             complement(20313..21047)
                     /locus_tag="ACHGMT_000952"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFN6834718.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            21176..21799
                     /locus_tag="ACHGMT_000953"
     CDS             21176..21799
                     /locus_tag="ACHGMT_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFN6834719.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            21991..23112
                     /locus_tag="ACHGMT_000954"
     CDS             21991..23112
                     /locus_tag="ACHGMT_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFN6834720.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            23119..26247
                     /locus_tag="ACHGMT_000955"
     CDS             23119..26247
                     /locus_tag="ACHGMT_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN6834721.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            26266..29460
                     /locus_tag="ACHGMT_000956"
     CDS             26266..29460
                     /locus_tag="ACHGMT_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFN6834722.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(29606..30313)
                     /locus_tag="ACHGMT_000957"
     CDS             complement(29606..30313)
                     /locus_tag="ACHGMT_000957"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN6834723.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         9987 a         5565 c         6496 g         8559 t
ORIGIN      
        1 aaaaaacgtc ctagtcaatc agttggccga gcggctggcg gctgatctgc tcctgatcaa
       61 taacgagtgc ttcttggaag tgttgctgca aaaagtagcg gtcatggctg actgctagga
      121 gcgttccttg atagtcttgc agcatttctt caatttcttc tcgagcatag ctgtcgaggt
      181 gattggttgg ttcatccaaa atcaggacgt tgattttttc taaaaagagt ttcagcaacg
      241 ccaaacggat tttttcacca ccggataaat ctttgatccg tttacccaca tcgctttgat
      301 aaaagccact tttggctaat tgccgtcgag cgacttcttc gtttccagtc aatgtgatgg
      361 cgtaagccag caaacggcta tcgggatcgg caaactgcag ctgctgcggc aaataaccga
      421 tttgcaagct actacctaat ttgatggtgc caccgtaagg cttttcctca ccgagtaata
      481 atttgagtaa ggttgatttt cctgcaccat tgtcgccgat gatggctaaa tgatctccac
      541 gatagactgc aaaactgctg tctttgaata gtacttgatc agcgtactgc tggtgcaagt
      601 tttgaatgat gaaaacttct ttgccagatt ttccaccatt agacaagctt tgcagccgat
      661 ttttcggggg aggtggcggt ttgacgagtg tcagtttcgc caggcggcgt tccagttctt
      721 tggctttttt gaagaaggac tcattgtctc cttcatgtgc ccactgacga aaacgacgaa
      781 tcatgacttt caaccgctgg acttcttttt gctgcagttc gtaatttttt gtcagctcag
      841 ccaagcgagc ttctttcaag actcgaaaac ggctgtagtt tccgggatac tcaatcaatg
      901 caccttcttc cagttcccat gtttttgtgg tgacttgatc gagaaattgc cgatcatgag
      961 aaacgaccag aaccgatttt tttgtattct gtaaatagtt ttccaaccag cgaatactgt
     1021 taagatctaa atgattggtc ggttcatcga gtaaaagtaa atcggcatcg gctgctaaaa
     1081 ttttggctaa agcgacttta actcgttcgc ctccgctaag ttctgttaac cgtgcttcct
     1141 gacggtgtgg aatccctaag ccttgcaaga tcgctgcaat ccggtcttct aatacgtaac
     1201 cgcctagttc ttcaaagcga tgctgcagct caccgtaacg ggccagacac ttctctaagt
     1261 ttgtatctgg ttctgtcatc aatgcttcgt agcgactcat ttgctgtttg aggtcctgta
     1321 tctcagcggc agaatggagc aagtagtggt tgacggtcgc ttcggaaact gtaagttctt
     1381 gttcgaccgt cccgatcttt aagccttttt tgcgactgat aacaccgctg tcgacacctt
     1441 ctctgccggt caagatttgt aaaaaggttg atttgccagt tccgttgata ccgactaaga
     1501 caattttttc accagcttca atttgacagg ataaatcctc aaataatgga ttcccgccga
     1561 ttgttttttt aatagaaatt gcttgtatga tcatgatctc tctccttgag accactcaaa
     1621 aaggccatgg ttatccatgg cctgtgttat ttaaactcag attatcatga gaaaaatacc
     1681 ttgagatggc cattgatttt acgtgaattc aactgttttg gacaaacttg tcccgtcaac
     1741 agcaactgta aaaccaattg catggctaaa tgcgatatgg tatcgttggt aattttcatt
     1801 acttgttatt ttagtcatgc gcatcatagt attttccctc cttcgtgtag ttgtcttttc
     1861 cagtgtacct gaaagtgtca gaaagttcaa ctagcgctga catttactta atatttatcg
     1921 atgtgtaatt ctgtcagaga gcttttttta tctgactgag ggtaatgtct cgtggaccac
     1981 atcattgtat tctgagctaa accagcgttc aggcgaaatc atatggtgtt tgataaaaaa
     2041 ctttgtctac atgacttcct tttgccattt gcttaatacc gttgcattaa acagctcttc
     2101 tctttggaaa tctcaccaac atcgcgttca ggagtatcgt tgtggttgca atggcaagcg
     2161 gtagcctttc atcaattctt gaatgattta ccgaatgtag cacttgcctt ctcgcggtac
     2221 ccgaacccga aataaaattt tagtttggcg ataaaatcgg tatccgccat cgatacttca
     2281 tattttttcg tatcagattc atcggaaact tccacattga cgaaccaata agctttggca
     2341 tgcttaggtc gttttaaacc acaccagggc gactaatcgc cctggtgttc actttttttt
     2401 attttacttg aactcattga ctgttttccc tttattttaa acattataaa gaccgatgag
     2461 tgcttgccgc gtcttttgtt tttatcacgg ttttttgtgc aaaaaaacct gccgatagtt
     2521 ggcaggtcgt ctacgtgtta agctttggtg tggtctttag ctggcaattg gttttctaaa
     2581 tcagttcgga caacaagcac atcgcaagct gcgtttctaa taacatattc agaaacagaa
     2641 ccaataaata aacgttctac agcattcaat cctgtggctc ctaacataat caaatctact
     2701 tgattatctt ctgggatttc tctggcaata atttgttttg gtgagccata ttctacgaca
     2761 gaggttacat tattcaagcc agctttcttc gcattgcttt cataatctgc caatgtttgc
     2821 tttgccattt cagtagcttg ttctgcaagc attccatcaa aggatgaaac ggtttggaaa
     2881 gaacgtgtgt caatgacatg tgctaataga agttctccgt tatttcggat agctacattg
     2941 accgcctttt taaaagctaa ttcagcttca tcagaaccat ctacggcaac catgattttt
     3001 ttgtattgtt gtaacataag cattcactcc tcaagtcaat cgtattgtac tcttttattg
     3061 tagaacactt tagggaaaaa gagaattatt tactgctttg gttcagcaat tttttttgca
     3121 aatcggatac tgaaggtggc agaaacaata attacgatca gtaaaaagac caaggcaata
     3181 aagcgttgat tgaaaaaagc tgttaaaata cccattacgc ctaaaacagc atatgcagct
     3241 ccgtaaaatt gtaggaatcc ttgattttta tcatttttgt tgatcaatac aaaaaagata
     3301 tcctgtttat taaataaata atagctaacg aataataata ctaaagtggc gataatcatt
     3361 aaaatcctaa tcatgaacaa atcctccaaa ataagcaaca aatagaaaaa gcaatttgcc
     3421 aagagcaaat tgctttattc tttaaaagat ggtaatctcc gttaatgccg ttgtttgtcc
     3481 tgtagtattg atcccgcgaa ttcaagcagg tgggtcccat ggtcgtagct tcgaactacc
     3541 aaatgataag gcatgttacc agcagtgaca caagacgggt cccaagatat caataaaaat
     3601 gttcggtcaa cacacaaaga ggacaattcg aacatcacag atttcccacc tctatagtag
     3661 catatgaatt taaaaagagc aatgagagag ctcgcagaaa cagaaccgtt ttcttgtcta
     3721 gttttttgga ggatgctttt tccactcttt tatacggtaa tactgttgac ccagagcctg
     3781 ttcatatttc cctgtatctt ttggctgata atactgagca ttcttcagtt tatctggtaa
     3841 atattgctga ttgacccaag cctgatcaaa atgatgagga tactgataac ccacgccacg
     3901 gttgagtgat ttcgcacctt tgtaatggct atctctcaaa tgatcaggaa catctccagc
     3961 tttgccctct cgaatatcag caagtgctgc atctaatgcc atgtaggcag aattggattt
     4021 tggcgacaag caaagatcga cgacgacgtc tgctagtggg atacgtgcct caggcaatcc
     4081 caatttttca gcagctaata cagcgttcac tgtacgagcc gcagcagcag gattacctaa
     4141 acctatatct tcataaccga taaccatcag acgccggcaa atacttgcca gatcacctgc
     4201 ctcaactaaa cgggcgagat aatgaagtgc agcatccaca tcacttccac gaatcgattt
     4261 ttgaaaagcc gaaataacgt cataatgagc atctccattt ttgtcatggg taagagcttt
     4321 tctttggaca cattcttcaa tgatcgacag tgtaagatga atccttcctt ctttatctga
     4381 aggagtcgat agtgtagcca gttccagccc attcaatgca ctccgcaaat cgccgttcgt
     4441 tgcacgcgaa aggtgaagaa gagcatcttc gtctaactgg attgcttgtt gtccaaggcc
     4501 gcgttcttta tcccttaaag catgttcgac tgccaactgg atgtcttgtt ctgttaatgg
     4561 tttgacttca aagatctgtg tacggctgcg gatcgctgga ttgatggtaa tataaggatt
     4621 ctcagtagtc gctccgatca agatgattcg gccactttct aaatgaggaa gcagaaaatc
     4681 ctgttttgtt ttatctaatc ggtgtacttc atccaataag aggatcactg taccactcat
     4741 ttttgcttct tccgctacaa cttgtagatc cttttttgaa tctgtagcgg cgtttagcat
     4801 gcgaaatgca taattagtag agccggcgat cgcacttgcg atactcgttt ttccagtacc
     4861 aggaggtcca tatagaatca tggaagagag catgcgagca tcgaccatgc ggcgtatgat
     4921 ttttccttcc ccgactagat gctgctgacc aacgacttca tcgatcgttc gaggtctcat
     4981 gcgataagcc aatggctgtt gcataggatt ttcctccttc ttgtttttac gtcccttaat
     5041 tatatcatat ttagcaagat gcctagaaag aacagccttc tgttgttttg ttttcatagc
     5101 ttatccaact aatattgtgt atgataaagt aggtgagaaa aatgacggat aaacaaaaga
     5161 tagatgaatt tttttggaat aaaacgacgc cagaagtggc acgggattta ttggggatgt
     5221 atttggagca tgacacacca gaaggaagat tagctggata tattgtagac gcggaagcat
     5281 acctaggtcc agaagacgag gccgcccata gctatggttt aagaagaaca ccgcgtgtgc
     5341 gggcaatgta tgaaaaacca ggtacgatct acctttacac gatgcatact catcggattt
     5401 tgaacatcat tactcagcca gaagggattc ctcaaggagt aatgatacgt gcaatcgaac
     5461 ccgccgcaat gattgatcaa atgagtaaaa atcgtggcgg aaaaactggt ccagatatca
     5521 gcaacggtcc gggaaaatta gtggaagcat tagcaattcc tcaagaactt tatggtcagt
     5581 ctatagcaga tagttcgtta cgcttagttt ttgagaaaaa aaagacacca aaaaagatca
     5641 atgctttgcc tagaatcggt atcccaaaca aaggtgtatg gacagaaaag ccgttgcgtt
     5701 ttgttgtgtc gggaaatcct tatatctctt tacaaagaaa aaaccaaatc gagaaagatt
     5761 ggggttggag gaaagaaaat gaaaaagaag gatctattaa catatttaga tgaaataatt
     5821 gaaaaacaag tgacagacta tgatgtagct ttagattggg atacaaaaaa tcatacgatc
     5881 gagatcgttg ttcgcttgtt tgcagaaaat aaagcacaat ttgagattga tgatgcagaa
     5941 gggatcgttt cgcaagaacc aattatcgaa tttgaagacg gcgtacttct ttttaatcct
     6001 cagaaatctg tttttgatga acgcaattat ctagcagtaa tcccttatga aggaaaaaaa
     6061 gggttggcaa aatcagtagc tgattcttta gtggaatatc taaatgaagt attagctcaa
     6121 gggcaaagcg atttgcttga ttttttggat gaagacgatg acgaagctgt attcgagctt
     6181 cactgggata atcagcaact agcagaatta gtagaagtaa aacagcaaaa tgaagcagat
     6241 acttatttac cgtacccaag ttattaagga gtgacagata tgaaatggaa tgaagtaaaa
     6301 gtagaaactg aaagtgaagc agtcgaagca gttgctaata ttttgatgga ggcaggggcg
     6361 agtggtgtcg cgattgaaga ttctttagat gttgaacatt ttcagtcgga tcaatatggt
     6421 gagattttag ataaaaatga ttttacatcg ctgaaagaag gcgcactagt catggcttat
     6481 tttccggaaa cgactttctt accggaaatc ttgcctttta tcaaagatcg tatcacacaa
     6541 ttacctgagt ttggtctagc tattggaaaa aataaagtaa cagttagtga agtagaggaa
     6601 agcaactggg caacagcctg gaagaaatac tatcatccgg tgcgtattac gcgaatgctg
     6661 acggttgcgc caagctggga aaactaccag acagaagatc ctctagaaaa aattatcaca
     6721 ctagatcctg gtatggcatt tggcacaggc actcatccca cgacttcctt gacattgaca
     6781 gcactagaaa cagtcttgcg tggtggtgaa acattgctag atgtgggaac tgggtcgggc
     6841 gtgctaagta ttgcagcaaa atatcttggt gcaaaagatg tctatgcctt tgatttagac
     6901 gaagtagccg ttcgttcggc gaaagaaaat atggatatga atgaaatagc caaagatgtc
     6961 catgtatcag ctaacgatct tttgacagga atcgagattg aaagtgatgt aatcgttgcg
     7021 aacattttag cagatatcat cttattgatg atcccagatg cttggcgttt actgaagcaa
     7081 acaggcacgc tgatcgtttc aggaatcatt gaagagaaga agcaaatggt tctggatgcc
     7141 atgttagaac aagggtttat cgtggatcaa attttccagc aaaaagactg gtatgcaatt
     7201 attttgaaaa aaccagaggt ggaatagatg cagcggtatt ttttaaaaga atcatatgaa
     7261 tcaaaagact attttgtttt agaaggggaa aactatcacc acattgtacg ggttatgagg
     7321 atggaacccg aggatgaagt ttttttggca ttcaatgatc aaatagccat tgttgcagaa
     7381 atcacggaga ttactgaaga ggctgtttat ctgaaagaaa ttaaaaaaga agaaaatcaa
     7441 aaggaactgc caagttcgat taccatagcg agtggctatc ctaaagggga aaagctggat
     7501 tggatcgttc agaaatccac tgaattagga gcgcaagctt ttcttggttt cccggcatca
     7561 gcctctgtcg ttaaatggga tgcaaaaaaa cgtatgaaac gacaacaacg gcttgaaaag
     7621 attgctcaag aggcagcgga acaatcccat cggcagatcg tgccatcagt agagttattg
     7681 gaaacgaaac aagatttatt tgagcggcta gatgattatg acgtggtctt ggttgcttat
     7741 gaggaatcag caaagcaagg cgaaaaatca aatgcagcca aaattcttag tgaaacaaaa
     7801 ccagatagtc gaatcttagc tgtctttgga ccagaaggtg gattatcccg cgatgaagtc
     7861 gattattttg tagaacacaa agggatactt tgtggattag gaccaagaat ccttcgtaca
     7921 gaaacagcac ctctttattt attaagtgct gctagctatc aatgggaatt aatgaatcct
     7981 tctttcagct aacgttcttc attaataaaa gagtatcgac agaaagaatt gaaaaaatag
     8041 tcaacaattt gtataataga aacaattatt tatacaatgg atacgaaaaa tcgtttgaaa
     8101 aagcttttaa aaagcgaaca tttccgtaaa aaaacgtata actgcacgga ttttgggaca
     8161 attatcaaac agatatttgt cccgattccc gttttaccga aaaaagaaag ggggatcgat
     8221 tcctgtgaca aaagaagaaa ttttaacggg acccggtgtc ataaagctcg ttagtcaata
     8281 catgggtccc gaacatgtag catttgttga gaaagcatgc gaatacgcga ctgctgcaca
     8341 tgacggacag tttagaaaat caggcgaacc ctacattatt catcctatcc aagtcgcagg
     8401 tatattagca gatttaaaaa tggatcccca tacagtggct acaggcttct tacatgatgt
     8461 tgttgaagat acagaaatca ctttagaaga tctgagagaa gaatttggcg atgacgttgc
     8521 tatgttagtg gacggcgtaa ccaaattagg gaaaataaaa tataaatccc acgaagagca
     8581 gctagcagaa aaccaccgaa agatgctgct agcaatggct caagatttac gagttatcat
     8641 ggtcaaatta gctgatagac tacataacat gcgtacgttg aagcacctgc gagaagataa
     8701 gcaaagaagg atcgctcagg aaactttaga aatctatgcg cctcttgcac atcgtctagg
     8761 gatcagccgg atcaaatggg aattagaaga tacagcactt cgttatctaa atccaaaaca
     8821 gtattaccgt atcgtccact tgatgcagac gaagagagaa gaacgtgaaa aatacgtaag
     8881 tggtactgtt gaagatattc gaatagcgac agaagagctg gggatttttg cagaaatcta
     8941 tggacggcca aaacacattt attcaatcta tcgtaaaatg aaagatcaga aaaagcagtt
     9001 caacgaaatt tatgacttgc tagctattcg agtgatcgta gattcaatca aggattgtta
     9061 tgctgtatta ggagcaatcc acacaaaatg gaaaccaatg cccggcagat ttaaggatta
     9121 tattgccatg ccaaaagcta atatgtacca atctttgcat actaccgtga tcggtccggc
     9181 aggaaatccg gtagaaattc aaatccgaac acaggaaatg catgaaatcg ctgaattcgg
     9241 ggttgctgca cactgggcct ataaagaagg aaaaaatgaa aaagtagaac cagatggtat
     9301 gacgaaacaa ttaagctggt tccatgagat actcgaactt caagacgaaa gctatgatgc
     9361 ttctgaattt atggaaggcg taaaaggaga tatctttagt gataaagtct acgtcttcac
     9421 accaaaagga gacgttactg agttaccaaa aggatccgga ccattggact ttgcatacag
     9481 tatccataca gatatcggta acaaaaccac tggtgcaaaa gtaaatggca aaatggtgca
     9541 gcttgattac aaattgaaaa acggagatat cattgagatc atgacttctc caaattcatt
     9601 tggcccaagt cgcgactggt tgaaattagt tgctactagc aaagcaagaa ataagatcaa
     9661 acgtttcttc aaagcccaag atcgagaaga aaatgtgatc aaaggccacg aatccgtggt
     9721 caaatgtatt acagatctag gatttacgcc taaagatatt ttgacgaaga acaaactgca
     9781 agaagcactc gatcgtttta attatcaaac agaagatgat ctctatgcag ctgtagggta
     9841 tggagaagtt agccccttga cgatggccaa tcgtctgact gaaaaagaac gtaaagaaca
     9901 aaaaatcgag cagcaaaagc aagaagcaga agaaatcatg aatcagccga aaaaagaacc
     9961 tgacaaaatg aaagtacgtc atgaaggtgg cgttgtcatt caaggggtag aaaacttact
    10021 aattcgtatc agtcgctgct gtaatccgat tcctggtgat gatatcgttg gttatatcac
    10081 taaaggcaga gggatatcca ttcatcgtcg agattgtccg aatgttcagc ctgacaaacc
    10141 aaatgtagca gaacgtttga ttgaagtcga atgggaagat acatcgaata cacgaaaaga
    10201 gtatgatgca gatttggaaa tttacggcta taatcgttca ggcttattga atgatgtact
    10261 tcaaacagtc aatgcgctaa cgaaaaatct caacagcgtt gaagcacgga cgaataaaga
    10321 taaaatggcg acgatccatt tgacggttgg tatccagaat ttatcccacc taaagagtat
    10381 cgtggataaa atcaaagcag tacctgatgt ctacagtgta cgccggacga atggatagga
    10441 ggacagatat gagagcagtt gttcaacggg taagtaaagc tgctgtgtca gtcgatcagc
    10501 aagaaattgg aaaaattggc tacggcctac ttgttttatt aggcattcat gaaaccgata
    10561 cgcaaaaaga tgtggactat ctggtaaaaa aaattgcaca aatgcgtatt tttgaagatg
    10621 aacaaggaaa aatgaatatt agtgtagaag atgtgggagg acaaatttta agtatctctc
    10681 aattcacact gtttgcggat acgaaaaaag gaaatcgacc aagctttgtg gaagccgcac
    10741 gaccagaaac agctattcct ttatacgaat cattcaatga aggaatcaga agtcgcggca
    10801 tcacagtcgc taccggacaa ttcggagcag atatgtcggt ttctctcgtc aatgatggac
    10861 ctgtaacgat cattattgat tctaaaaaca aataaagtaa tctggttttc actagtaaaa
    10921 actatgaaac acgtcgctta agttagactg ataaactcta acttaagtgg cttttttgtc
    10981 tgcaaaaagc gcttcttttg tcagcagtcg gaaaaaaagt taagcttaaa ttatacaagt
    11041 tattttttat aggaggttgc tgaagatgaa agaagtacca gtagtagata tcaaaatcac
    11101 agatttaaaa aaattgtatc aaaaaggata caacatgttg gaggagttac gccatgaagc
    11161 ggacgctccc gtcgtcaaag caaagatttt caataaagaa gctatcacga tctacggatc
    11221 atcagcagca aaagtatttt atgatccaag gaatttcaaa cgaaaaggtg ctatgccaaa
    11281 attagtattg aagacacttt ttggtcaagg tggagtacag actttggatg gagctgctca
    11341 tcaccatcgg aaaaatattt ttatggatct aatgacaccg gaaagaatgg aagattatca
    11401 tcgtatctta gataagaatc tgacacaagc acttgaagca cagcatggtc aatttgaact
    11461 atttgattta agtaagatgg ttttctttac atctatttgc gagtgggcag gtatcaattt
    11521 atcagcgatt tctaaagatg aggtcgaaaa attagcagaa tatcaaatct ctatgatcag
    11581 cggaacgttt acttctccga ttgatcacat aaaaggtgtc gaaaatcgca aaaaatcaga
    11641 aaaatgggcg caaggcttga ttgaagaagc tcgacaaaat cctgtggctg gaaaagaaaa
    11701 tgtggcatta tatgcttttg ccaatgccac agatttagat ggtcagcttt tacctctgga
    11761 agtggcagct gttgaattat tgaacatcat ccgtccaacg gttgctctaa ctgtgtgggc
    11821 ggcattgatg ggacatgcac ttttcagtag accagatttg tatcagcaat taaaaaatga
    11881 cttttcgacg ttacaagatc cgtttattca agaaatgaga cgctattatc ctttcttccc
    11941 aatgctacct gcgatttcgc tgaaagaagt agaagtagat ggttatcgta taccagaagg
    12001 cagttgggtt attttagatt tatatggaac agaccatgat gaacgaacag ttgaagcgcc
    12061 agattccttt atgatcaaac ggtatgtagg gaaagcaaaa gatatttcct ataaagaaga
    12121 atatgagatg attgcacaag gcgggggaaa ttttcgacaa atgcatcgct gtgcaggaga
    12181 atggatcact cttcatagtc tacgtgtgtt ttccgatcag ctagtaaata agtttgagtt
    12241 ctctgtacct gaacaagatt ggacgattcc attcaatcag tttccgactt atccgaatag
    12301 ccgagcgcta ctttacaaga attagattag cataaactta tcgaaaaaaa ggaagatcaa
    12361 acagtgctga tcttcctttt ttcatattac tataaattga taaggtcaat tgatcaaaat
    12421 ccacgaaatg cttcatcaat ggtttgataa tcttcattgg ataaatgcaa atctaaagct
    12481 tggacattgt cagatacttg ctctggacgc ttagctccag gtataactac agacactcgt
    12541 ggattcttca tgtaccaagc taaaacaagt tgcgcaggag tggcttggta tcttttagca
    12601 atggggcgaa gtacatcgac agcggttaaa atttctctga aacgttctcc ttggaaatct
    12661 ggatttcttt ttcgaggatc tccttcacca aattgttttt cctcaccaag ttcatatttt
    12721 cctgtcagca aaccggaagc aagaggaaaa tagggaacaa aactgatttt gtttttttca
    12781 agataaggga ataattcttt ttcagcctgc cgatggatca aactgtattt atcttctact
    12841 acatcgacat atccatctgc attcgcttct ttcaattgtt ctaacgtgaa gtttgacaca
    12901 ccgactgctc ggatttttcc tgcttctttt aattcgtgca atgtagcaac tgattcattt
    12961 tttggtgtgc tttcatccgg aaagtggata taaaaaatgt cgatgtaatc agtttgcaga
    13021 cgttttagtg catcttccac cgattgtttg agaaactcag gagaattatc aaatgttcgc
    13081 cctttatttg gtacatgagc agcttttgta gcgatgacga cacgtgagcg atcatattct
    13141 ttttccttca gtacctcgcc aatcagttct tcggaacggc cgtttccgta agcataagcg
    13201 gtatcgatca aattgatccc gctattcaaa gctgtacgaa ctacttgttt tcctgtctca
    13261 tcttccaaac cgtcaaaaag attatgtcct ccaacggcat ttgctcctaa tccaagctgt
    13321 tctgcgtaga cttgtgaatg tccgattctt atttctgcca tagctgattc ctcctttttg
    13381 tctagtctaa aacaaaatac caaaaagctg aactaaaacg tttttgtgag gggaaatagt
    13441 cagatatctc ttgactttga gactggtttt tagtaggtta taagtaatat cgaatgtcaa
    13501 tgaaagagcg agtaagtttt tgaaacttta caagagagga tcatcgaggc tgaaaatgat
    13561 ctaaagtttc attagcctaa agacactctg gagatggacg agtgaaaagc cgtcccgcgt
    13621 acgagcgtta ttcgtaagag gaaagagaaa tctttcaaaa ttaggtggta ccgtgtatga
    13681 atttcattca cccttgttca tgatgaacaa gggtgatttt tttatttaca tagctaagaa
    13741 agggagagaa aactatgagt ttccaaagac caaaaggaac aaatgatatt ttgccaggcg
    13801 aatcagaaaa atggcagttt gtcgaagaaa cagcacgttt attattcaga gattatcagt
    13861 ataatgaaat gcgtacaccg atttttgaac actatgaagt gatatcccga agtgtagggg
    13921 atacaacgga tatcgtttca aaagagatgt atgattttta tgacaaaggg gaacgccatg
    13981 tgacgcttcg accagaaggt acagcaccaa tcgttcgttc ttttgttgag cataaattgt
    14041 tcggcccaga gtatgcgaaa ccgttcaaaa ctttttatat gggaccaatg tttcgctacg
    14101 aacgccctca aaaaggacgg ttacgtcaat tccatcaaat cggagtagaa gcatttggat
    14161 cagaaaaccc tgcaactgat gtggagagca tggtgatggc acttgatttc ttcaaacagt
    14221 tggggatcac gcaaatccgg ctagtcatca attcattagg tgataaagaa acgcgtcaag
    14281 cctatcgtca agcattgatc gactatcttt taccttttga atcagagctg agtgaagact
    14341 ctaaacgccg ccttcatgaa aatccattac gcgtattgga cagtaaagac aaacgagatc
    14401 aaaaatttgt ggcagaagct ccatctatct tagattatct aagcgagcct gctcaaaaac
    14461 attttgatac cgttgtttcc atgctggatg cattggctgt tccttatgaa atcgacagta
    14521 atatggttcg cggactagat tattacacac atacgatttt tgaaatcatg agtgacgcac
    14581 caaaaatggg ggcacaagca acgatttgcg caggcggacg ttatgacaat ctagtggaag
    14641 aactaggtgg tccttcaaca cctggcttcg gatttgcaat gggtatcgag cgattactct
    14701 tgactatgga ggcagaggaa gtagttatcc ctgcatttaa tgagttggat gcttacgtgg
    14761 tcgcactcgg agacgaaaca aatatcgaag cattgaaagt ggtccaagcg attcgaaact
    14821 ttggtttttc agctgaccgc gacttcatga accgtaaagc aaaagcgcaa tttaagacag
    14881 cagataaagc gaatgccaaa ttagtattgg tgatcggtga agatgagctt gctagtcaaa
    14941 cgattaatgt caaagcaatg gcaagccgaa aagaaaaaag ttttccatta gaagagatat
    15001 atgaacattt tgatgaagtc tatgatgaaa tgactttatt cgagtaggag gaaaaaaata
    15061 aatggcacaa agaacgatct attgcggatt agtttccgaa caacaattag aacaaacagt
    15121 tacattaaaa ggatgggtac aaaaaagacg agaccttggg ggagtaatct ttatcgacct
    15181 acgtgatcgt gaaggaatcg ttcaaatcgt ctttaatcct gaaatcaaca aagaagcatg
    15241 ggaaatcgcg gataaatgtc gcagtgaata cgtgatcgaa gtgactgggg aagttagaag
    15301 acgcggagaa aatgcagtaa atcctaaaat ggccactggt gaattcgaag tgattgcaag
    15361 tgaaatcgtg attttgaatc aagcaaaaac cccgccattt caaattgaag atgatcaagc
    15421 aatcagtgac gaaatacgtt tgaaatatcg ttatttggat ctgcgtcgtc caaaaatgac
    15481 taaaaacatc aaattaagaa atgcgacaac aaaagcgatt cgtcattatt tagatgataa
    15541 tgactttctc gatatcgaaa caccttacct agggaaatct acaccagaag gcgcaagaga
    15601 ctacctagtt ccttcacgtg tccatgctgg acatttctat gcgttgccgc aatctcctca
    15661 attgtttaaa caattgctga tgaatgctgg attcgaccgt tattatcaaa tcgttcgttg
    15721 tttccgcgat gaagatttac gtggagaccg tcagcctgaa tttacgcaag tagatattga
    15781 aacaacgttc ttaacagcag aagaaatcca aacttacaca gagggattga ttgcgaaggt
    15841 aatgaaagaa gttcgcggta tcgaagtcac attgccattt cctcgtatga cctatgatga
    15901 agcaatggct cgctacggca gcgacaaacc ggatacacgt tttgatatgg aattgatcga
    15961 tctaagcgat acagtcaaag aagtagaatt caaagtcttc caaatggctc ttgaaaacgg
    16021 cggtgtcgtg aaagcattga atgctaaagg cgctgcagat cgctactcac gaaaagacat
    16081 ggatcagtta ggtcaatatg taggacaatt cggagctaag gggcttgcat ggctgaaagt
    16141 agaagaagac ggattgaaag gtccaattgc taaattcatg ggagaagcaa cagaagcaat
    16201 cattaaagca acagatgcaa aaccaggtga cttgctgatg tttggcgcag ataaaagtga
    16261 aatcgtcgct gctgcattag gtgctatccg gacacgttta ggaaaagaat taggactaat
    16321 tgatgaatcg aaattcaatt tcttatgggt aactgattgg ccacaatttg aattttcaga
    16381 agaagaaggt cgatatgtct ctgctcacca tccattcacg atgccaaaag aagaagatat
    16441 cccgttattg gctgaagacc ctgcaaaagt ctatgcagaa gcttatgata tcgtcttgaa
    16501 cggttatgaa ctaggcggag gttctatccg tattcatact cgtgagcttc aagaaaaagt
    16561 actagaaact ctaggcttta caaaagaaca aatggaagaa caatttggtt tcttgttaac
    16621 agctttagat tacggattcc ctccacatgg cggtattgca ttaggattgg accgttttgc
    16681 aatgttatta gccggagaag ataatattcg tgaagtcatt gcctttccta aaaacggaaa
    16741 agcagcagat gtgatgagtg atgcaccaag tatggtttcg cctttacaat tgttcgaatt
    16801 gaatattgat gtaacgaata tagaagaata gccgataaaa aggtaaaaag gatttgtcga
    16861 atacgcttct tttgacaacc ttgatttaat tgaaaaacac tttataatga agccataaca
    16921 tataaagcgg ggcttttcca tgaaaaaaac attcgaaaat tgggcttatc atgattacac
    16981 gaccaagctt tcggtatcta tcgtttatgc gttgatcgca tcagtcgcat tgaatttctt
    17041 ttatcagcca ggacatattt attcaagtgg tattactggt ctagctcaga ttttgacgac
    17101 tttgtcaaaa tcactgatag gattcgaagt accggtatca acaactttgt atttactgaa
    17161 tataccttta tttgtattgg cgtggttaaa aatcgggaag aagtttacaa ttttcacttt
    17221 tatcacagtc actttgacct ccctttgtat ccatatttta ccggaaacag tcctatctac
    17281 cgatccaatc atctgtgcgt tgtttggggg ggctgtgatg ggactaggta ttgggctagc
    17341 gttgagaaac ggcctatcat cgggcggtct agatatcgtg agtatcacga ttcgtaaaaa
    17401 aacaggaaag tcgattggtt ctatttcgat ctacttcaat gcattgatcg tttttgttgc
    17461 cggaattcta tttggctggc aatatatgtt ttacagtacg ttgtctattt tcgtcagtgg
    17521 aaaagtaact gatgtcgttt atactaaaca aaagaagatg caggtaatga tcgtaacgaa
    17581 acatccagaa aaagtagtaa atgaaatcca aaactgtatg cgtagaggga tcacgatcat
    17641 caatgaagca gaaggtgctt ataagcatga taagcagaca gtattgatga cgatcgttac
    17701 acgatacgaa ctgcctctac ttagagaagc aatgaaagct tctgatccac aagcttttgt
    17761 aagtattgca gaaaacgtcc agatacttgg tcgtttctat gaggatggca tttagatcga
    17821 gtttgacaaa aattcatgaa atactgctgt agacagacgt tcgtttgtct gcagtttttt
    17881 tctaccatag tagaatcagc aaagaaaaac tgctttttta tttgaaattt tcttaaagtt
    17941 gaaaaaaagc atgtttattt ttttaaattt atgggatgat agaaaaaaat agtagagaaa
    18001 ggacacgatt ctacttgcta ttttcactta atacccgtat attacgaatg aaaaaagttt
    18061 atgaaaagtt ggaatattga atggaaaaac aaaaaaggtt aaaaaacagc cgctacatca
    18121 ctgggtttga tgggctccgt tcattagctg tggtgggtgt tatcttctat caccttctgc
    18181 caactagtat gaaaggcggc tatttgggcg ttccgatttt tttcgtcgta tctggttatt
    18241 tgatcacgga tctgctgcgg caggaatggg aacaaaatgg aaaaatcaat atttggcaat
    18301 tttatgtcag aagaatgaag agactctatc cggcacttgt ttttatgctt gtgacagctt
    18361 cggcttatat cactttgttt cagcgagggc tgttaaacaa tcttcgagga gttgtggtca
    18421 gcagcttact gtatgtcaac aactggtggc aaatcaataa cggtttatct tatttcgatc
    18481 gatttgctaa tgagtcaccg tttacgcata tctggtcttt agcagtagaa ggtcaaaact
    18541 atttggtttg gccgatcttg tttgtattgt tgatggtatt tgttcgtaaa agaaaatgga
    18601 tcttttatac tgtgttaggt acatccttcc tctccgccat cttgatgatg atcttgtata
    18661 caccaggagg cgatccaacc cgtgtttatt acggtaccga cactcgtctg ttttctatat
    18721 ggttgggaag tgcgcttgcg tttgtttggc caagtacgcg tttaaagaaa aacatcccga
    18781 aacaagcgaa aaatattctg aatatcgctg gaggaatctc gcttttggca ctgattcttt
    18841 gcttcttctt tttagatgat cattacaatt tcatctatta cggaggaatg ctcctagtga
    18901 gtctgttatg cgtagtgcta gttgcagtga cagcacaccc cggagcaagt ttgaatcgat
    18961 ggctgacaaa tccagtattt acttggattg gcaaacgaag ctatggcatt tatctgtatc
    19021 agtttccagt tatgattttt tatgaagcaa aggtgactga tattgccgat catgttttgt
    19081 tgcatacttt agtggaaatc acattgattc taatcatcag tgaactttct tatcgatatg
    19141 tggagcgtcc gctagcacgt tttgattatg gtcgtacatt ccaagtgatg aaaggatggt
    19201 tcagcaaacc gatcatcagt aagagcaaac catgggttgt tccaggcaca ttacttgtag
    19261 caattgcact cgttggtttc gttacggcac cgaaaaacgc tgtaacagcc gatcaaaaac
    19321 aactgcaggc aaaaatcgct gaaagtaaaa aattagcaga agaaaccaga aacaattcag
    19381 aaaacaccac tagttcggtt gaccaagcga tccttgataa gtacgagttg tccaaggcac
    19441 aagggaataa ggcacaaaaa ttagaactaa cggcatttgg tgattcagtt atgttggatg
    19501 ctgcaacgga tctacaagaa ctttatccaa aagtagtggt agatggagat gtcggacgac
    19561 aattatatgc cagtgtaccg tacattgaaa aattaaagga acaagatttg ttgaaagaca
    19621 ctgttctgat cggactagga acgaatggct cattctcgga agctcagttt gatagtgtga
    19681 tggatgcaat cggtgatcga aaagtatact gggtcaatgt ccgagtaccg acgaagcgct
    19741 ggcaaaatga cgtgaatgcg atgcttgaac agatgacgaa aaaatacgac aatatgacgc
    19801 tgattgattg gtacagctac agtaatgacc atagtgactg gttttatgat gatcaagtcc
    19861 atccaaatcc tgaagggatg acacattaca ttcacttagt cagcgaagcc ttgctaggag
    19921 gaagttcaag tacgaacagc agtgagacaa acgtgacaga atgatagcta caaaaaactt
    19981 caggcaaaaa catttgtttt tgcctgaagt ttttttatat ttaccgtgtt tttaacattc
    20041 atatagattc aggaagcaaa acggttgagt gtgtctgtaa ataacgttct ttttgttttc
    20101 gggtgagttt tttgaatgca gcttctgctt ttgttgcttc gcttcttgaa gaaaatactt
    20161 ctgcatggat catcttaaca ggtcttcttt tttgaagacg agtatattta gcaccagtac
    20221 cattgttatg ttcctctaat cgtctagaaa gatcggttgt atatccacca taaaatgtct
    20281 gatctttaca gaataatacg taaaaataat gttcatttgc catagagcat ctcacgaatc
    20341 tctgcagtgt attctccatc catattgtaa gtgatcaatg gtggagcgac tttgaatccg
    20401 tctaatttgc cttctttgat tccttcaact aacaaaatat ttgcctcttt tccaggtttt
    20461 gggtaaacaa attggatcct tttaggtgca attcgatagt cttccatgac ctgaatgatt
    20521 tctaagaacc gatcaggacg atggaccata gtaaagcgtc catttgtttt tagtagctta
    20581 cttgaaatat cgatcacttc ttctaacgtc gtatgaatct catgacgggc gattgccagg
    20641 tgtttattag ggttcgtttt atttgttggc agccctttga aataaggtgg attacagacg
    20701 actaagtcac aagaattatg tcgaacgaca gataagctgt ctttcagatc gatggtgtgg
    20761 acagtcattt gttcatttaa atggttaagc gcgatactac gtcttgccat gtctgctaga
    20821 cgaggttgaa gttcgatagc gtcgatagcc gcctgggttc gtttacttaa aaaaagtcca
    20881 actgcgccat ttcctgcaca aaggtcaaca atcttccctt ttttaggtac acgggaaaag
    20941 tgagcaagca gcacagcatc taaagaaaaa gaaaacactt tggaactttg gataatcttg
    21001 atatcttctg cgtataattg gtcgattcgt tcgtcttcgt gaagcatgga ttccctccaa
    21061 aatgaaaaaa atagtcgtct gtattatact atttttatta aaaagaacca aggtggcttg
    21121 tcaaacctct attttttatg catactatga ccataagaag ggaaaggaag agactatgtt
    21181 ttatcgattt atgcgcgggg tagttcgagt actattagcc ttagttaatg gaaatgcccg
    21241 ctatgagaat aaagaagttt taccgcaaga tgagaattac atactagttg ctccccatcg
    21301 tacttggtgg gacccattat atctggcggt agctgcagca cccaaggaat tcagctttat
    21361 ggcaaaagaa gaactgttca aaaatccagt tctgcgattt atattaactc gatccaatgc
    21421 ttttccagtt aaaagagaaa atccaggacc aagcgtcatt aaaacacctg taaagatttt
    21481 gaaatcgaca aatttgagtt tgatcatgtt tcccagtggg acaagacatg ctacagaact
    21541 taaaggcgga atggcattga tttccagaat gtctaaagcg aaaatcgtgc cagctgtata
    21601 tcaaggtccg ttgacacttg gggatctgtt caagcgtcag agagtgacag tgcgtttcgg
    21661 cgaaccaatc gacttatcag atatcaagaa aacggataaa gaaggtatgg cggagatcga
    21721 acgacgaaca caagcagctt ttgacaaatt ggacaaagaa gtgaaccctg atttcaaata
    21781 tgaagtcaaa aaaaaataaa atgataagtt tatgcgtggg cggtcttttg aatctactgt
    21841 cctgttcagt tcagactttt tcaatgaaaa cgagggagag acgtcaagtc tgccctcttt
    21901 ttgcgttcca atatgtaaaa tagaagacag gtgtcatgta atggatcaaa gtgttataat
    21961 aattagaagg acattttttg aggtgattga atgcgttttt tacatacagc cgattggcat
    22021 atcggaaaaa aactacaagg ttatgacttg attgaagacc agcgtcatgt actatcttct
    22081 atactagata tcgcgaaaga agaaaaagta gaggcaatcg tgattgccgg agacttatat
    22141 gataggagtg tcccagctgt tgatgccgta gaattataca atgaattgat ggtcgattgg
    22201 aatttaaagg aaaagatccc gatttttgct atatcaggaa atcacgatag cagtacgaga
    22261 ctttcagcgg gtacgccatg gttcagttat tcgaactatt ttttgcgaac acagctagca
    22321 gaagcatttg aaccagtcga gtttaaaaac gtacaatttt tctttcttcc ttactttgaa
    22381 ccaattgcag cgagattata ttttgaagat gacaatatcc gaacgatcaa gcaagcggta
    22441 gccaaggtga tagataaaat gcaggagcta tttgattcag aaaaaaaaca agtattgatt
    22501 agccattttt ttgtaatggg aagtgtaaaa tctgattcgg aaacgaaaat cgaagtaggc
    22561 gggttggatt cgatccctgg tgaactactt acaccgtttg attatgtagc actaggacat
    22621 ttgcataatc aagctgcttt gagacaggat aatgctcgct atagcggttc cccattgaaa
    22681 ttctctttat ctgagatcaa ccaaaaaaaa ggcgtatgga tcgttgacct gacaacaggc
    22741 ctggagttga attttaggga gatcgaaccc ctgcatgata ttgaagaaat aacagcgagt
    22801 ttcaaagaat tgctggatcc tgccttttat cagtccgtca atcgggagaa ttacatctat
    22861 atctatctca ctgatagaac gatcatcccg aatatgatga accagcttcg gcaaatatat
    22921 cctcgtattt tggggattga acgggcaaac ggtcgagcag aacaaacaaa gcgcaaacga
    22981 aaagtactag aaatgaaaga tcctcaaaaa ttagcagaag cattttttag tgaaatgacg
    23041 gaagagcagc taactgcaaa ccaagaaaaa tggctcggag aaacgttgca agaaatcatg
    23101 gaaatggaat gatcaagaat gaaaccgaaa aaattacaac ttaaaaattt tggtcctttt
    23161 atcaatgaga ccgtagattt ttcacgtttg acagaagcac cgcttttttt gatcagcggg
    23221 aagacaggag ctggcaaaac gacgattttt gatgggatca ccttcgcact ttttggtgaa
    23281 acttccggaa gacttcgctc tggaaaagag atgcgttctt tgtttgctac accagacgaa
    23341 gaaacaagcg tgactttttc ttttgaacat cagcagatga gctatgaaat cgaacgaaaa
    23401 ccagaacaag ttttagcaaa acgaaaagga aacggaacaa aaaaacaggc agccaaagta
    23461 acgttgacca tttttgatgg gaaaggaaaa gaaataaaac aaatcacaaa acgtacagaa
    23521 gcagatcagt tgatcagaga actgatgaat ctcgatgcga aacagttttc tcaaatcgtt
    23581 ttactacccc aaggagaatt ccgtaatttt ttggtttcat caagcagtga taaagagact
    23641 gttttacgtc atttattcgg tactcaattt ttccaacagt tcaatgagcg attaaaagaa
    23701 aaagcaaaac aacagcaaca aaaattggat catttagagc aagagctgca tcttctgcaa
    23761 aaaaggtttg ttttagtcaa agaagaggaa ccagcagcag ggtttgaagc cgttttagcc
    23821 caatggacaa accatcaggc aatactcgaa aaacaaatca cggaagaaaa agaagctcta
    23881 acggaacttc aggaaaaaca aaagcaacta gaaaattcct actatcgttt tgaaagcttg
    23941 aaaaaaagtt atgaagaaaa aacgatatta ataaacaagc aaaaggaatt agctgctcaa
    24001 aaagacgaaa tagaggaaaa gaaacgatgg attgggtatt atgaatttac gcaacgatta
    24061 gtcgaaccaa tccagcgcct acaagaaata aaagttgaaa aaatagaact gtcacaaaaa
    24121 agtagtgagc aaacaaagaa actagcgatg actacgcaga attatgaaga gtggcaaaaa
    24181 aatgaagcaa accgcgtacg ccgacaaaca aaaatcaaag aagctgctca aaaactgcaa
    24241 aacaaacaga tgatggtgcc aattgtcgaa tccttacgta gtaaaaaaca agacgcagaa
    24301 caattaacaa tttcattgga taaagaagaa ttgaatgccg agaagctgta tcaaaatctc
    24361 gctatctatc aggaaagaac tcgaattctt caagaaaatt tactggatca ggaaaaactg
    24421 caagaagatc gtcttgcttt tggacagtta aaatatctag atgaaaagca gcaagaaata
    24481 gaaaaacaaa aagcaaaaga cactgggtcg ctcgcacaga taagagaaga actcaaaaaa
    24541 gcaactgaag aaataacaaa aaatagtcag ctattgaaag aacagacaga acaagcagaa
    24601 cagctgaaaa gccaatgggc aaagcagcaa attgctcgtc ttcagctttt tttgcgtcca
    24661 ggggagccat gtcttgtttg tggatcaacg gaacacccca tacaacaatt aaaacacgaa
    24721 cagccaaata aggaagatat cttaaaagta gaacaacaac tggcagaagc tgagtctgct
    24781 gtagaaagaa ctcagcaaat cattgccaaa agtgaagcac atgaaaataa tcttcaacaa
    24841 caattatcag atttagaaca aaaaatcatg gcaacgaata tacaattggc tgaacaagaa
    24901 gaacacttgc aacaggcttt gaaaaaggca tttcctgaga taacagaaca aaaaacaaaa
    24961 acaactcttg tagtaatcga acaatcttta gatagacgaa aaagcaagat ggccaatgca
    25021 agaatagagc tggaagaaat aagacaacaa gcagaaaagc tcgaacaaca attagctgtc
    25081 tacaaacagc agcagacaga aaaaaataat cagctccagc aactgcaagg agaaatcaaa
    25141 agtttagaac aacaattgca agatacggat agtaactgtt tagaagagga gatacgacga
    25201 cttgaaaaac agttagaaga agactcgaat caactagctg ttgagcagaa agcaggagtg
    25261 caattgttgc aagatcttca tacgcttcgg acacagcttg atcttcaaca tgaacaaata
    25321 gaaaaaatag atgaaaaaat agaaaaggaa caagaaagaa tagctaaaaa gttagaaagc
    25381 cagtcgtatt tttcaacaat tgaagaaatc tgtgcatttg cacaacagca agcacaatat
    25441 aaagaaaacc aacgtacagt tacttcttat catgaagagc aattagtgac agctgaccgg
    25501 ctgcgtcaat tagaaaatat ccagctaact gagacattcc cagatacaga agagatcagc
    25561 caacagttag aagaaataag acgtatgatc gaaataaagc aagaaaagat atacggttta
    25621 caagaacaaa aaattagtaa tcaaaaaatc tacgatgaat gtctttcgat ttatcacaca
    25681 agccagacga aattagatga gcttacgcaa ttacagcagc tttcgcaaac attcaacgga
    25741 gaaaatccta agaagactag tttggaacgg tatgtgcttc aggtttatct tcaagaagtg
    25801 ttacaagtag caaatgatca tctccaacgt ttaacgaaaa atcgttatca atttgagcta
    25861 gcggatacca ttggcagtta tcgagggaaa accggattag aaatcaatat ctatgatgat
    25921 caggcaggta tgtctcgaag tgcgcatacc ctgtctggcg gtgagagttt tattgcagca
    25981 ctttctttag cattagctct tgccgatgtg atccagacac aagctggtgg tgtagcaatc
    26041 gatgcattgt ttattgatga aggatttggg tcattagatg aagaagcttt ggaaatggca
    26101 atggaagctt tagaaacgat cgaaagtgaa ggaagaatga tcggcatcat tagtcatgta
    26161 agagaattga aagaacgagt attacaacaa ataagaattg atacagaagg tagcggacaa
    26221 agcaaaatac gctatcaact tggataaaaa gggagattgg taactatgaa atggagtatt
    26281 ccagaaaaag tcattgcacg tggacgtcaa tacttgcaag aaggccgagt gttatcggtt
    26341 gtccctgacc aagaagaaaa cgtgtggcat gcagaagtct tgggcagtga attgtatatg
    26401 gttgatttgg atggtacagc aaaagagata gattactgcc aatgtcccta ttgggaggaa
    26461 cataaatatt gtaagcatac agttgctgtg gaactttatt tacggtcgaa aaatctatct
    26521 cgtatcatga aaaaagacca gccaatgcta gaaccggtaa aagcaacaag tgagggagaa
    26581 atcattgcaa aaggtttttc tcgtctaaaa acacaaaaca atcctcaagc ggtgcaacct
    26641 ttgattattg aataccaagt agaaacgatt gagactaatc agtatcaccc agagctttct
    26701 attttggcac tttacttgcg tatcggttca ttggcatcac cgaaaaaaac atatatcgta
    26761 aaaaacatct atgatttttt acaagcttac acagaaaaag aaacttacac tgttaataaa
    26821 cagtaccagt ttcgtctaga taaacgggcg tttcaaaaag aagatcaaaa aatactcacg
    26881 catcttgcag gaagtgctca gacgcatcaa ttattaggta cgaacggctt acaagtcaaa
    26941 ggaaaactag ataaaagata tctgcttctc ccagtagaac aagcccgctt cctgttagag
    27001 cagatgaatc agactacacg tttgcatctg cagataaatg aacaaaacga tcaaggaata
    27061 caattttccg atcaaaacaa acctttatca ttcaaagtgg aaaaatttgg agaagattat
    27121 ttgctgcaag cattgaatga ttttgatttt ttctttatgc attatcagtg gggagttatc
    27181 caagggacat tttattctct gacgaaatat caacaaacgg tttatcaaac ttggaaacaa
    27241 ttgattcgcc gattggagaa accagaagta gtgttcaaga aaaaagaatt accgtctttg
    27301 tttaaagaga ttatccctct gctgtcagag atcggcgacg tagctattgc ccctgcagta
    27361 gaagaggaag tagcagatat ccccgtcgaa tttattttct ttttccgaaa agcaaaagga
    27421 aaaatccaag ctcgagtcga tttcgtctat ggcgaagtca tctattcgac tgacccgaag
    27481 catgaggtct cgacagaaca ggcttttcag gtgttacgtg acacagtaga agagaaacga
    27541 gtacttgatc tattcagaat gtatcgctac cagaaaaatg aaactggtta tgaacgtcca
    27601 ttgccaagcg gggaagaact atatgccttt ttccgcacag aactatctgt cttccgtcag
    27661 tttggtgaag tcagacttgg aagaaaattg cgagagttgt atttagatgc gcataaacat
    27721 caaccgaaaa tcgaagtaac cgagaccggt tcatggctcg atattcgatt cgatgtgaca
    27781 gggatagagg agcaagaaat cgatcatgtc ctgcaaagct tgctgagaaa tgatgctttt
    27841 tatacattag aaaatggtca ggttctttct tttgattcag aagaatttca acaaacaagc
    27901 caagtgttgc aacaattacg ggaatcgata cgtacagaag aaggaacgat ccatgtacct
    27961 aaaaatcaag gattgatcat tcaaaatcaa ttggaaaaaa gcaatgctac ttttagtgaa
    28021 tcgttccaaa cgatggtaca agatctcatt catccagaaa gataccaggc acagcttccc
    28081 aaaggattga atgcaacgat gagagattat caaaagcaag gttttcgatg gttgaagatg
    28141 ttaggtcatt atcagtttgg cggtatttta gccgatgaaa tgggacttgg gaaaacacta
    28201 cagacaattg cctttttact ttcggaaaaa gaagaacgga aaagcttctc tgctttgatc
    28261 gtagcgcctg cgagcttaat ttacaactgg caggcagagg tgagaaaatt cgccccttct
    28321 ttaagtatcc aagtaatcaa tgggaataaa aaagaacgtg aagaattact ggctaaagac
    28381 acagatatcc gagtgacttc ttatgccagc ttaagacagg atctagctaa ttatcagtcg
    28441 caaaaaattg attatttgat tcttgatgag gcacaaatgg tcaaaaatag cagtacgaaa
    28501 actgcacaag cattgagaga attagctgta cctcagcggt ttgcactgag tgggacacca
    28561 atcgagaata atttagaaga attgtggtca ttgtttgcaa cgattatgcc aggatttttc
    28621 ccgactaaaa caaaattcag ggaacttgcc gcagaagaaa ttgcgcaaat gattcgaccg
    28681 tttattttac ggcgagataa acaaactgtg ttgaaagact tgccggaaaa aacagagatg
    28741 aatctatatt ctgcacttac agaagaacaa aaaactgttt atttagctta cttgcgtcaa
    28801 atgcgtgaag aaatcacatc aatggattct gaagcattca agaaaaaccg gatcgggata
    28861 cttgctggtc tgacaagact gcgacaaatt tgctgcgatc ctcgtctgtt tattgaagat
    28921 taccaaggcg gttccggcaa gctcgaacaa gtaaaagatc tactggtagc tgccaaagag
    28981 aacaaacgaa gagttttgct gttttctcaa tttacaggga tgctgactat cttgcaagaa
    29041 gagttagccg agctagggat ctcgacattt tatttaagag gcagtacgaa accacaagat
    29101 cggttaagta tggtagacgc atttaatgct ggcgaaaaag atgtttttct gatttcctta
    29161 aaggctgggg gaactggatt gaatctcacg ggagcagata cggtcatctt gtatgacctt
    29221 tggtggaatc ctgccattga agaacaagct gctggacgtg cacatcggat cggacaaaaa
    29281 aatgtggtag aagtgtggcg tatgatctct gaaggaacga ttgaggaacg catggattca
    29341 ctccagcaag aaaaacgcga actgttccaa aaagtgatcc aaggtaatga agaacagttg
    29401 acgaaactga ctgaagaaga catccgcatg attttaagtg taggagaaat cgaggagtaa
    29461 gagactcgaa aaagcgttca gtaatgggta agaaagcctt tctaaagaag cgattgttta
    29521 tttgatttaa aaaatagcaa ggaggctggg attccatttt atcccagctt ccttgctatc
    29581 atttttttat ttctattttt aaaggttata caaagattgt cttcattgtc tctaacagct
    29641 gatcaaaaga agaaatctcg atatcgcaaa ttggagtctg ttcaattttg cgttctcgat
    29701 ggttgaacca aagcgcttgc cagttagcac tttttgcacc tagaacatca ttgtcgtaat
    29761 tatcgccaac gtataaggtc tcttctggaa gcatatgaaa ttctttctca gctagctgaa
    29821 aaatctcttt ttctggtttt tggaaacctg ttgcttgaga aatgagcata ttgcgggaag
    29881 gaatccaatt gttcaattga agttgcttca gtttttttgt ctgatggtca gtcggcccat
    29941 ttgtgataat tcctaatggg acattctttt ccttcaagaa atcgagggtt tttttgacct
    30001 cctcatgcaa gcagatatta tcaagttctt cctcgtaaat tttttgaaat aagaggccat
    30061 cttcttccgt aatatgaggg tagtctaaat cttttagtga ttggctgatc cgatgtgctc
    30121 gcatatattc tagtgtccaa tctcctgcca ttacttttgg gaagttttca tcactatgat
    30181 ggcggaaccg gatatatagt ggatgcatat ctgcatctga aacaagcgga acgactcttt
    30241 tgaccgcgtt tcgaaatggc tgctgctgat catacatcgt atcatcaaca tcaaacacaa
    30301 ctgatttaat caaaatttta cacgcctttc ggttttcatt gattttttca caaatccttg
    30361 actattgtaa cagaaaaaat tgtctaaaaa agctgataaa taaaggtttt tcttactata
    30421 tttctatttc cttgacagaa taaggttttt atatttcagt ttttttcact aaaaatcgtg
    30481 aaacacgtca ataagtttga tgaacaaatt aatcattatg gtagaagtcg attgatttat
    30541 tttaatgcaa tctctgtgga attttcgaaa gatttatttt tttaatacag tctcaatata
    30601 actattc
//
LOCUS       DAZBSA010000023        30410 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000023 DAZBSA010000000
VERSION     DAZBSA010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.023"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..292)
                     /locus_tag="ACHGMT_000958"
     CDS             complement(<1..292)
                     /locus_tag="ACHGMT_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834724.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVA"
     gene            complement(280..909)
                     /gene="pyrE"
                     /locus_tag="ACHGMT_000959"
     CDS             complement(280..909)
                     /gene="pyrE"
                     /locus_tag="ACHGMT_000959"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFN6834725.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(916..1632)
                     /gene="pyrF"
                     /locus_tag="ACHGMT_000960"
     CDS             complement(916..1632)
                     /gene="pyrF"
                     /locus_tag="ACHGMT_000960"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFN6834726.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(1629..2561)
                     /locus_tag="ACHGMT_000961"
     CDS             complement(1629..2561)
                     /locus_tag="ACHGMT_000961"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFN6834727.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(2558..3334)
                     /locus_tag="ACHGMT_000962"
     CDS             complement(2558..3334)
                     /locus_tag="ACHGMT_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFN6834728.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(3388..6573)
                     /gene="carB"
                     /locus_tag="ACHGMT_000963"
     CDS             complement(3388..6573)
                     /gene="carB"
                     /locus_tag="ACHGMT_000963"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFN6834729.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(6578..7654)
                     /locus_tag="ACHGMT_000964"
     CDS             complement(6578..7654)
                     /locus_tag="ACHGMT_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFN6834730.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(7659..8939)
                     /locus_tag="ACHGMT_000965"
     CDS             complement(7659..8939)
                     /locus_tag="ACHGMT_000965"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFN6834731.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(8958..9884)
                     /locus_tag="ACHGMT_000966"
     CDS             complement(8958..9884)
                     /locus_tag="ACHGMT_000966"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFN6834732.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(9897..11180)
                     /locus_tag="ACHGMT_000967"
     CDS             complement(9897..11180)
                     /locus_tag="ACHGMT_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFN6834733.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(11194..11733)
                     /gene="pyrR"
                     /locus_tag="ACHGMT_000968"
     CDS             complement(11194..11733)
                     /gene="pyrR"
                     /locus_tag="ACHGMT_000968"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFN6834734.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(11968..12879)
                     /locus_tag="ACHGMT_000969"
     CDS             complement(11968..12879)
                     /locus_tag="ACHGMT_000969"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFN6834735.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(12876..13340)
                     /gene="lspA"
                     /locus_tag="ACHGMT_000970"
     CDS             complement(12876..13340)
                     /gene="lspA"
                     /locus_tag="ACHGMT_000970"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFN6834736.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(13345..14043)
                     /locus_tag="ACHGMT_000971"
     CDS             complement(13345..14043)
                     /locus_tag="ACHGMT_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFN6834737.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(14030..14416)
                     /locus_tag="ACHGMT_000972"
     CDS             complement(14030..14416)
                     /locus_tag="ACHGMT_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834738.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(14563..16233)
                     /locus_tag="ACHGMT_000973"
     CDS             complement(14563..16233)
                     /locus_tag="ACHGMT_000973"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFN6834739.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            16416..16649
                     /locus_tag="ACHGMT_000974"
     CDS             16416..16649
                     /locus_tag="ACHGMT_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN6834740.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(16748..17179)
                     /locus_tag="ACHGMT_000975"
     CDS             complement(16748..17179)
                     /locus_tag="ACHGMT_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFN6834741.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            17258..17671
                     /locus_tag="ACHGMT_000976"
     CDS             17258..17671
                     /locus_tag="ACHGMT_000976"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFN6834742.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(17709..18857)
                     /locus_tag="ACHGMT_000977"
     CDS             complement(17709..18857)
                     /locus_tag="ACHGMT_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFN6834743.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(18979..19575)
                     /locus_tag="ACHGMT_000978"
     CDS             complement(18979..19575)
                     /locus_tag="ACHGMT_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFN6834744.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(19775..22417)
                     /gene="alaS"
                     /locus_tag="ACHGMT_000979"
     CDS             complement(19775..22417)
                     /gene="alaS"
                     /locus_tag="ACHGMT_000979"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFN6834745.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            22805..23236
                     /locus_tag="ACHGMT_000980"
     CDS             22805..23236
                     /locus_tag="ACHGMT_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6834746.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(23281..24630)
                     /locus_tag="ACHGMT_000981"
     CDS             complement(23281..24630)
                     /locus_tag="ACHGMT_000981"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFN6834747.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(24700..25863)
                     /locus_tag="ACHGMT_000982"
     CDS             complement(24700..25863)
                     /locus_tag="ACHGMT_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFN6834748.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            25992..26315
                     /locus_tag="ACHGMT_000983"
     CDS             25992..26315
                     /locus_tag="ACHGMT_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834749.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(26498..27847)
                     /locus_tag="ACHGMT_000984"
     CDS             complement(26498..27847)
                     /locus_tag="ACHGMT_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFN6834750.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(27916..28869)
                     /locus_tag="ACHGMT_000985"
     CDS             complement(27916..28869)
                     /locus_tag="ACHGMT_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFN6834751.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(28971..30161)
                     /locus_tag="ACHGMT_000986"
     CDS             complement(28971..30161)
                     /locus_tag="ACHGMT_000986"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFN6834752.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         8548 a         6678 c         5343 g         9841 t
ORIGIN      
        1 tcgcaacatt tccgctcttt accatttttg aaacagctcc cttaccaaaa aatagagaaa
       61 atctatatat aatagaaaaa agagaagaga ttattctctt ctttctgcta accagtctta
      121 gaaaaatgaa ttttaaaaaa atcaaaaact gatcacgata gaagtttctt ttcatacctg
      181 atgaaaagat gcttctcgca atcagttttt ggatttttgc tattccaatt gatgattctg
      241 gagacacaaa tcggatttct ctatcttcat caagcgcgct taaatctgcc aattttcagg
      301 atcttttttc cattctttta gcagagttaa ttcttgctca tcgatataac gatcttctaa
      361 agctgcttca atcaatacag aataattggt caacgtcata agagggattt cggctttttc
      421 gaaatttgct tttccttttg gcaactcata cgtaaagata gccgcaacac caaggatatc
      481 cgcaccttct ctttttgctg cttcagctgc ttccaagaca cttccgcctg tagaaatcaa
      541 atcctcgatc acaaccatct tctgaccttc aaatatacgt ccttcgattt ggttaccttt
      601 cccatgatct ttcgccttac ttcgaatata gaccattggc aaatccagta tttccgctac
      661 ccatgcggca tgagggattc ctgctgttgc agttccagca atcacttgta cttcagggta
      721 agcttctttg attttgcttg ctaatccttt tgcgatctct tttcggactt ttggatagct
      781 catcgtgatt cggttatcac agtaaattgg gcttttgatt ccacttgccc atgtaaaagg
      841 attacttgga tttaaaaata ctgcttcaat ttctaataag tcttttgcga tactttgttc
      901 aacattcatt ttaggttagc tccattctgt ttgaatcgtt tgatatgctt tataaggatt
      961 agttgactga gtgattggtc gacctataac gataaaagtt gagccgatct tttttgcctg
     1021 agaaggtgtg actactcttg tctgatctcc tgcgactgtt ccttctggtc ttatacccgg
     1081 agttaagcaa acaaattcgt ctcctgccat ttctttgatc gcttctactt cccaagcaga
     1141 agaaaccaca ccatctaatc ctgctttttt ggcacagcta gcataatgga tcacactttt
     1201 ttctaaagaa acttcgatta gttgatcggc atgcatttct tcttcacttg tagaagtaag
     1261 ctgagtgata gctagaagta gcggacgttt tccaccttct ggagtcccct cttctaatcc
     1321 tcgcattgcc gcctccatca tacgaatacc gccagcggca tgaacacagg tgagatcaac
     1381 accaagcttt gccaatcccc gcattgcttt ttcgaccgta ttcggaatat cgtgaagttt
     1441 cagatctaaa aatacattat gaccagcctc ttttagataa cgtacgattt caggtccttc
     1501 ttggtagaac aattccattc cgattttcac aaacaatttt tcttctttcg ggaaaaatcg
     1561 aagaaagtct tctacctctt gcctagttga aaaatctaat gcaataattg gtcgttggtc
     1621 tagttgattc atcgttcacg ttcctctctc acttctttga ttaatctttc cagcgattcg
     1681 atgcctagtt cgctcattcg cacagggagc tctttaatta gtttaggaca gatatacgga
     1741 tcggtgaaat tcgcggtccc tatagctaca gcacttgctc cagccataaa catctcaaga
     1801 acatcatcca ctgtctgcac gccgcccatc ccaatgattg gaaggctgga tatacttgct
     1861 acttggtgaa tcaaacgaat agcgacaggc ttgattgctg gtccggagag tccacctgtt
     1921 tgattggcta aaatcggttt tcttgtctta aggtcaatcc gcataccaag aagagtattg
     1981 atcatcgtaa acccatctgc ccctccagct tcgattgcct gagcgattgg aacgatgtct
     2041 gttacatttg gggacaattt gacatagaca ggaacagaag aaactttctt tactgcttgt
     2101 gttaattgaa aagcaacatc agaatctgtt ccaaaagcaa ttccgccatg tttgacattt
     2161 gggcaagaaa tgttcagctc gattgctttc acatttggtg cttcgccaat tttgctacaa
     2221 acttctacat agtcttcttc acaagcacca gcaacatttg cgatgatggg taactcattg
     2281 tactcctcta attctggaag aagtttatgc ataacaacgt ctagaccagg attttgcaaa
     2341 ccaattgcat tcaacatgcc agatggtgtt tccgccactc ttggcgtggg attgccaaag
     2401 cgggcatttg gagtcgttgc cttgaccatg atcgatccaa gctgattcaa atcatagtat
     2461 tttccgtatt ctttgccaaa accgaaacag ccgcttgccg gcatgatagg attttttaat
     2521 tctaggccag gaagtttgac cgctaaagga ttctcattca tataattacc tctcctattt
     2581 taaaaatcgg tccttcgtca caaactttca cactttttgt tcctgtttca tcttccggcg
     2641 tatgacaaac gcaagcatag caagccccta tcccacatgc catgcgggat tctaatgata
     2701 actggacatt ttgatgcgaa gaaaacagct gttccactgt cttcaataac ccgtttgaac
     2761 cacatgcaaa aacagctgta ggttccgtct tcgttcctaa caataaatta ccgacatttc
     2821 cgtgcatacc gtatgttcca tcgtcagtag cgattcttgt ttctcctaga cgattaaatt
     2881 ctttttcata ataaacgact tcatgtgtgg caaaacctaa aaaatgcgtt ggcttaattc
     2941 ctttttcgac taactgtttt gataactcat atagaggagg gacaccgatt cctccaccga
     3001 ttataaatac ttcgtctcct gtatgaagat tggacaaatc aaaaccattg cctaaaggac
     3061 ccatgacatc taaatggtct ccggcagaaa gagtagagaa gactctagtc ccctcacctt
     3121 ccacccgata aatgatacgg caagtatttg tttcgtggtc gatatgattc aagctaatcg
     3181 gtcggcgtaa tagcaagtct gcacgcggca cgagaatatg caagaactgt cctggttgct
     3241 gcatttcttt tactagatca cctttcaatg tcatttcaaa aatctttggt gccagctgtt
     3301 tttgctgaac gatttccatc atttcttgtt tcatctgtcc cctcctgcaa cggtttttcg
     3361 tctttttgtc ttaacatgtg ctcgccgtta aatagggtta gttgaaaaag cacgggattc
     3421 catcactcga acgatcgcat ctgctgtgtc taaagaagta aacaatggaa taccatgttc
     3481 aactgcttct cttcggatga tgaaaccgtc tttagaagca tttccccgat ctttatcaat
     3541 cgtattgaca accacttgtg ttttaccatc tcttattaag tcgactacat ttttttctgc
     3601 tgcttcctca gaaattttcg ctactgtttc aacgaccaga ccattttttt cgaaataagc
     3661 agctgtatgt ttcgttccta tcagactata gccaatttct gtaaaacgtt tagctaattc
     3721 tagtgcttct tcttttgttt cgtccgcaat cgtaaataat acggctccat attcaggcaa
     3781 gtgtaatcca gaagcttcga atgccttgta caaagctttg tctaattgtc tgtcagatcc
     3841 cattacttct ccagtcgatt tcatttcagg ccctaagtaa gtatcaactt tctgcaattt
     3901 cgtaaaagaa aagacaggtg ctttaacatg aacttctttt gtttctggat aaagtccgtt
     3961 ttcataacca agtgtcttta gtttctctcc caaaatagct ttcgtcgcaa tttgtgccat
     4021 tggaattcct gtcaccttac ttaagaatgg aacagtccga cttgctcgag gattgacttc
     4081 gatcacataa acctcatttt catggatgac aaactggata ttcatcatcc cgacacaatt
     4141 caaacctaat gctaagcgga tcgtgtaatc ttcgatcgtt ttcttgatcg attcagataa
     4201 tgtttgaggt ggatacacag ccattgaatc ccctgaatgg acaccagcac gctcaatatg
     4261 ctccatgatc cctgggatga gtactgtttc accgtcacaa atcgcatcca cttcgcattc
     4321 accaccaagc aagtaacggt ccactagtac tggatgttct ggagatgctt tcacagcatg
     4381 ttccatgtaa tcttctaaat ctcgctggtt ttcaacgatt tccatcgcac gtccgcctaa
     4441 tacatagctt ggacgaacaa gaactggata accaattctc gaagcgataa ccacggcttc
     4501 ttctttactt gtagcagtat cccctaaagg ttgaggaata tccaactctt tcagcgcttg
     4561 ctcgaataaa tcacgatttt ccgcacgatc caagtcttcg atcgttgtcc ctaagatttt
     4621 tacaccagca gcagtcaaag gttcggctaa gttgattgct gtctgaccac caaattgaac
     4681 gattactcct atcggctgtt ctagctcgat cacattcatc acatcttcga aggtcaatgg
     4741 ctcaaaatat aatttatccg aaatagaaaa gtcagtcgaa actgtttcag gattgctgtt
     4801 catgatgatt gcttcgtatc ctgcctgctg gattgctttg acagaatgca cagtcgcata
     4861 gtcaaattca accccttgtc cgatacggat aggaccagat cccagtacta gaattgaggg
     4921 cttttctgat cgtgaacttt cattttcata ttcataggaa ctataaaaat aaggtgtttt
     4981 tgattcgaat tccgctgcac aagtgtcaac catcttatat acaggaatca aatcattttc
     5041 ttttctcatc tggcgaacgg cttcttcgtt tatttcccac agctccgcca cttttttgtc
     5101 tgtaaaaccg ttttgtttcg ctgttttcaa tatcttgtca tccaacggct gctgtttcaa
     5161 ctgctgttcg atttctacga tatgcaacag tttatctaaa aagaataaat cgatttttgt
     5221 caaatccgct aattcttcta tggtgtaccc tcttctaata gcttcagcta cataaaacag
     5281 gcgatcatct tgtgctcgaa ccactttttg gattaggtca tcgtccgtga tgtctgccaa
     5341 ctcaggaagt tcgatatggt gtgtaccgat ttctaaagaa cgcacagctt tcaataaaga
     5401 ctcttcgata ttccgaccga tagccatcac ttcaccagtt gctttcatct gcgtgcctaa
     5461 aagccgttct ccgttttcaa acttatcaaa cggccagcga ggaattttag ctacaacata
     5521 atctaatgca ggttcaaatt ctgcaaaagt cgttcctgta acaggatttt tcatttcgtc
     5581 taatgttaat ccaagcgcta ttttagcagc tagcttagcg atcggatagc cagtcgcctt
     5641 acttgctaac gcagatgaac gagaaacacg cggattgact tcaatcacat aataattaaa
     5701 actatgtgga tcaagagcga gttggacatt acagcctccc tcgatcttaa gtgctcgaat
     5761 aatcgaaaga gaagcatcac gaagcatttg atattcatgg tcagacaatg tttgactagg
     5821 ggcaaatacg atagaatcac ctgtatggat acccaccggg tcaaaatttt ccatattgca
     5881 gacaacgatt gcattatcag ccgaatctct catcacttca tattcaattt ctttaaaacc
     5941 tgcaatactt cgttcaatca aacattgggt agcgggggaa agacttaatc cattttctga
     6001 tacgatacgt aattcttctt cattttcaca catacctcca ccagttccgc ctaaagtaaa
     6061 ggctggacga acgatgacag gatagccgat tttatttgca aaatctacag cttcttctac
     6121 tgtattgacg atttcagact cggggattgg ctgattcaat tctttcatca actgtttgaa
     6181 tagatcacga tcttctgctt gatcgatcgc agaaagcttt gttcctagaa gttctatgtt
     6241 caattcttct aaaatacctg atgctgccag ctccattgcc atattgaggc ctgtctgtcc
     6301 gcctaaagtt ggaaggagag catctggacg ctcttttcga agaatccttg atacaaattc
     6361 aagtgtgatc ggttcgatat atacttgatc tgccacttct ttatccgtca tgatcgtcgc
     6421 aggattagaa tttactaaaa ccacttcata tccttcttct tttaaggcaa ggcaggcttg
     6481 tgttcctgcg tagtcaaatt ctgcggcttg accaatgatg atcggaccag atccaatcac
     6541 cataattttc ttgatatctg tacgtttcgg catctgttta ctgctccttt cctgcgtcca
     6601 tcatttccat aaattcatca aataaatgta gtgcatcatg cggaccaggg gcagcatctg
     6661 gatgaaactg cacactgaaa gcgggataat ctctgtgttt gactccttct actgttccgt
     6721 catttacttc tacatgagtt acgatgagtt tttctggatc aatcgtggct tcatcgactg
     6781 catagccatg attttgagaa gtaaaatcaa ttcttccggt cgctacttct cgtaccgggt
     6841 gatttaatcc tcgatgtccg aatttcattt tataggtatc tgcaccgttg gctaaagcaa
     6901 ataactggtg accgagacaa ataccaaaaa tcggtacttt tccttggatt tttttgatca
     6961 tttcgattgc ttctggcaca tcttttggat caccaggtcc attcgtcagc atgactccgt
     7021 ctggtgatag cgctaaaatc gtctcggcat ctgtgttata gggaagtact gtgaggttac
     7081 agtttctttt actcaattct cgtaaaatac tatgtttcaa gccgaagtca atgacaatga
     7141 ctttccgacc tgttcctgga cttggataag gtttgttagt tgagacttgc tgcacttgat
     7201 tggttgctag aacggttgct ttcagctgat caaatgcatg ttcgaaggaa tctccagcat
     7261 caactatact agctttcatc gttccgactt cacggatttt gcgagtaagg gcgcgcgtat
     7321 cgatacctga tattccagga attccttttt gtttcaaaaa tgaatctagc gtcatttggt
     7381 ttcgccagtt tgaagctaca cgtgcatgtt cttttactac tactcctttg catgttggag
     7441 aaatcgattc ataatcatct ctatttaccc cataattacc caccatagga taggtaaatg
     7501 taatgatttg accgttaaaa ctttgatcag tgatagtttc ttgataacca gtcatacttg
     7561 ttgtaaaaac gatttctccg aagacactag catctgcacc aaaagccttt ccttcgaaaa
     7621 ctgttccatc ttctaatata agcaaacgtt ccatactttt attctccctt ctgccacaca
     7681 agttttccgt tgacaaaagt catttgtgtt tcgcctttta ctttccagcc gataaaaggt
     7741 gtattctccc cttttgataa aaagtcttct ttatcgatcg tgcaagtttg tgtaatatca
     7801 aaaactgcta catcagcggg ggcaccaacc gttagcgttc ctgcattcaa accaaaaatc
     7861 tcagccggtt taactgccaa ccaattgatc acttgttcca acgtgaatcg accagtttct
     7921 acaaaatgag tatagatcag ttgaaacgct gtttcgcttc ctacgatacc aaatggtgat
     7981 ttcatgaaac tttggctttt ttcttctaat ccatgggggg catggtctgt agcgatgcaa
     8041 tcgatcgtac cgtcaagcaa gccttcgatc aaagcttctc tgtcctcccg tcctcttagc
     8101 ggcgggttca ttttccaaaa gccgaaatct tcgggaatat cttcgtcgat caaaataaga
     8161 tgatggggtg aaacttctgc tgttacgtgg attcctgctt tttttgcgtc tctgattaca
     8221 cggacacttt ctttagtaga aacatggcaa acatggtaat gaacaccagt tgcttcagct
     8281 agcaacagat ctctagcaat ttgagaagac tcggttacgc ttaagatccc gggaagacca
     8341 agttcttctg ccttcttgcc agcgtgcata acgccgccaa ataataaaga ttcatcttct
     8401 gtatgggcta ctagggcttt attgtttttt gcagcttctt tcatagccag atacatcgtc
     8461 ccagctgttt ggacacctac accgtcattt gtgaaagcaa aagcaccttc ttcaatcagt
     8521 gcttcttgat caactaattt ctcacttcgc agattttctg tgattggcgc atactgcaat
     8581 accttcacaa ctgcatcttt tctaatgatg tcatatactt gtctcagctt ttctgccgta
     8641 tcaggtacag gatttaaatt cggcatcgcg catacagtag tgaaaccgcc acgagcagct
     8701 gagcgagtcc cagcttcgat cgtctccttg tatgtaaatc ctggctctct caaatgaaca
     8761 tggacgtcca ctaaaccagg tgtgatcaat tgtccctttg catcaaacac ttcatcaaat
     8821 tcggcttctg agaatcctgt accgattgct ttgattcgcc catcttctat ccaaatttct
     8881 gctggtgtcg tcatattttt tcgtgtattg atctgaccgt ttttaatgag tgttttcatt
     8941 ttttcatcct cctataatta cgcttttcca tttagcactg cttctaagat tgccattctg
     9001 acgaatactc cattgctcat ttgctgaacg atccgcgact gtaatccttc cactaatgaa
     9061 tcagctaatt ctacatcacg gttaactggt gctgggtgca taatgattgc atgtttttgc
     9121 aacttcttcg cacgctcaac tgttaaccca tgttcctgat ggtagccttc ttttgagaaa
     9181 ctttcttttc catcatggcg ttcgtgttgc acccgaagca tcatcatgac atcaacttga
     9241 tctagtagtt catccaatgg catataatgt ccgtacactt cgaattcatc gtcgtaccat
     9301 tctctaggac ctgagaagaa tacttgtgcg cctagacgtt ttaacatctg catattggat
     9361 ttagctacgc gagagtgagt gatatcacct acgatggcaa ctttcagacc ttcaaaatga
     9421 ccaaattctt cataaatggt catcaaatca agcaagcatt gggttggatg ctgtccgctt
     9481 ccgtctcctc cattgataat cgaacactgg attgttttac tttgtatcag ttcgtcataa
     9541 tagttctcat caccatgacg gataaccgct acgtctacgc ctaatgctga catcgtcagt
     9601 actgtatcgt atagtgtttc ccctttttgg acagagcttg tgcttgcttc aaattcaatc
     9661 acttctagcc ctagtttttt ctccgctaca tcgaagcttt tatgtgtgcg agtactattt
     9721 tcgaaaaaga ggtttgtagc aaagtactgg cgcttttgcg gagtccaatt agcaccttgc
     9781 ttgaattcct gccctctgcg gatcaagccc ataacttctt gatcagtaag tgcctcaacg
     9841 gtcaataaat gctttaagct gatacgttct gatttgatga tcataatgtt tcctccttag
     9901 ttttcttcgc ttcgtgaagt ttctggtaag ataagattta aaatgatgcc aagtacagtt
     9961 gctaatgcca ttgccgacaa tgtaaatgtg cctacttcaa agacgagtcc gccaattcct
    10021 actactaaga taactgaagc aatcaacaga tttttctttt tatcaaaatc gattttgttc
    10081 tcgattaaaa tcttcattcc acttgcggca attactccaa ataagataaa actgattcct
    10141 gagatgactg gtccagggat acttaaaatc aaagcgctta attttcctac gaatcctaaa
    10201 atgactgcaa atactgcagc tccaccgatc acaaacacgc tatggacacg tgtgatagcc
    10261 agaacaccaa tgttttcacc gtaacttgtt acaggaggac ctccgacaaa ggctgccacg
    10321 atttgtgcta atccatcacc tgtcaacgtc ttatgaagac ccggttcttc aaagaaatta
    10381 cgtttagtta gtttattcaa taccataaga tggccgatat gctctgtcat cgtcacaaaa
    10441 gcgatgggtg ccattgttgt gattgctcca agatatagct ttggttcata ttgtacaaag
    10501 ggaatctcaa agttaggaac agcaaaccag gcagcgtcta cgattggctt agtatccacg
    10561 attccaaaca gcaaggctac gatgtatccg ctaataatac ctagtaaaat cggaatcaat
    10621 ccgagaaaac ctcttagcca catgttgaag aaaattgtta atcctaatgt gatcagcgct
    10681 acagcaatat acttgaaatc atacgtgccc tgattataca tagcatttgt tgctgcattt
    10741 gccgcaagtc ccaacccgat aaccatgatg actggaccga cgacgattgg aggcaagatt
    10801 ttatccagcc atgctgaacc gatctttttg atgatcagcg caacaatcaa atagactaat
    10861 ccagttgtga ctgctccttg tgcaatcgct ggataaccat ctgttttcat caaaagctgc
    10921 atcgctgcaa tgaaagcgaa gctgcttcct aagtacgcag gtatctttcc tttagtagta
    10981 aataagtaca ccaatgtacc tagtcctgaa ctgactagcg caatacctgg atcgatacct
    11041 actaagatgg ggactaacac ggttgctccg aacatcgtga acaagtgttg taaacttaat
    11101 cctacccaat gtcctggggt tgggcgatcg tggatatcta acaccacgtc atcattatga
    11161 aatccttttt tctccgacat gtttctctct cctctaatct tcctctttca aaatcatgat
    11221 gcgatcttgt ccatcaagct cttgcatctc tactaaaatc tcttccgccc gagatgttgg
    11281 aatgttcttc ccaacataat ctgcacggat tggcaattct cgatgcccac gatcgaccag
    11341 gactgctaat gaaatttttc ttggtctgcc aaaatccatt actgcgtcca ttgctgctcg
    11401 aatcgttcgt cctgtataca aaacatcatc aatcaaaata acttcttttc cttctaagga
    11461 aactgggatg tcagaagaat gaagttccgg ttcttctgga ttttcttttt tgtcatctcg
    11521 gtataaagtg atatccagtt ctcctacagg aatatcgata tcttctagct gcttcaggcg
    11581 ttcggcaatt cttgatgcaa tgtagattcc tcgtgttttg atacctacca atacgatatc
    11641 ttggatacta tggtttctct cgataatctc ataagtaatt cgagtcaatg ctcgcttcat
    11701 ggttacctga tctactactt cttttgcttg cattttcatt ccccctactt ttttgcataa
    11761 aaaaactcct gccctaagag gacaggagga atgtttagac tgagcgtata ctgatacact
    11821 gatagctata ctatgtcaat gagcaatcta cttatggcgc accatacgta tccttgctcc
    11881 cacccttctt agcctcacgg gactaaactt aaaggaattc tattaagttt tatgctttcg
    11941 ttgttaatta ctataccgtt atctaaatta aaatgcaatg ttttttctta atttttctaa
    12001 agttttttca aaaacttctg gaagaggtgc ttcaaagatc atcttttcgt ttgtagtcgg
    12061 gtgcgtaaaa cctaacattt gcgcatggag aaactgacca ttgcctttca aagttttctt
    12121 cggtccgtat actggatctc cagctaacgg ataaccgata tacttcatat gcacacggat
    12181 ctggtgtgtt cttccggttt ctagttttaa ttcaaccaga gtaaatcctt cgaaacgttc
    12241 caatacagta aaatgtgtga cagccgactt cccatcttct cttatcgcct gcattttccg
    12301 gttttccttc gaacggccga ttggcgcatt gatggttcct ttttcgtgag gaatctctcc
    12361 atggactaaa gcaacatatt tgcgcaaaga ggtcttatct tttaattgct gagctaatga
    12421 ttcgtgtgcc tggtcatttt tggcaaccat tagcagtccg gaagtatctt tgtcgattcg
    12481 gtgaacaata cctggacgaa taacgccgtt gatcgtagaa agatccttga tatggtacat
    12541 cagcgcattg accattgtgc catgcgtatg acctgctgaa ggatgaacga ccattccttg
    12601 aggtttgttt atcaccgcta catcttcatc ttcgtaaacg atcgtcaacg ggatattttc
    12661 tgggaccaca tctaatgttt ctggttcggg gatcaagaca gtaatctcgt ctccttgttt
    12721 tactttgtaa tttgctttga ctggctgtcc attgacagaa accgctccat ttttcagcca
    12781 cagctgaatt tgcgaacgac tgtaatcggt gaataaatta gtcaatactt tatcgatcct
    12841 accagattca tggtgaatcg tcgttttaag ttctgtcatt tttttctttg atccttttca
    12901 tctaaaataa tataaattaa catacaaatc actccacata caagtgtcac atctgctacg
    12961 ttaaaaatcg gaaagctcat aaaatctgtc tggaacatat ctactacata gccaagacga
    13021 atacggtcga taaaattgcc aatcgcacca gctagaatca aactcaatcc aatcatgaac
    13081 cagcgattac ctttttcacg atttttaatc atcaatgtga gaatcacaac gactgcaatg
    13141 agtgtcacta tagtaaagaa ccacatcttc ccttctagca tactccatgc ggctccagtg
    13201 tttcgaatat gattcagtga caaaatacct ggtataagat ttttcgtctc tccaagcgct
    13261 agattatcag tgatccacca tttgaccaac tgatccactc ctactagcaa agcgctgaat
    13321 atccaataaa taattaacaa aaaatcaatc cttttctatt tgtttataaa tatcacgagg
    13381 tcgtacgatc ccgagcagat taccatcggg attcccatct ttagtgataa gtacggcttc
    13441 caaagtccct tgctcacgaa acatcgcttc aacctcgtaa atatgtgttt tagctggaac
    13501 aaaatgataa tttgtgtact ttccgttttg gatgatcaaa tccaacgctc gtttatttgt
    13561 taaatcaacc ggaccttttt ggctttcttt tgctagccaa aacccgatat tacgtaaagt
    13621 aatcaatgct acaaattttc ctttattgta taacggaaat tgtgaatacc ttttttctgc
    13681 tactatcttt aacagttcaa gtaacggaag atcttgttca aaacctgtta cacgttttgc
    13741 aaatcgaggc agcacggttt caggctgaat cagttcttgt tcgatcagct ggattcgctt
    13801 gactgtccaa tcatttggct ctgcaatgac aaaatcgact gcgatccgat catggacgat
    13861 cgcattcctt aactgagcaa gctgtaaaag atcatcttcg tattttctga tcattaattg
    13921 ttttctttga gctaggcgtc tgacaagttc ggtaaatccc atatttcgtg catttcccat
    13981 ctcgtctctc atccattttt cgattcgatt gaaactgatt aaaaaaagat cagagtttgc
    14041 cataattttc ctccttgatc aaatatgccc ggttttttat atagaaaaag aacgcaatca
    14101 acacaataac tgcaaaaaaa ctgaaaaata aattttgtag gaaaacggcc cccaacccag
    14161 ttgctagaaa taaaaacgga tagatttgaa gaatcaagta attcattgtg atcacataag
    14221 caggctttgc ttctttcact gtataaacgg ggctttttag ccaataaaag gtgacctgca
    14281 tcgtaaaggg cacttccaga tcaagcgtta tcttttgttc atgactgaga acactgcgtt
    14341 tttcattgtt gattcttagc tgaatcgagc tacccatacc gtaaaaaccg gtttttctta
    14401 taaatgtcgt tttcatcata ccctcctatt atgctttagt atagctcaaa atacaataaa
    14461 gaaaaagaat gatcctagtg taaacaaaat taattcccta tcgtctaaaa aaagaagctg
    14521 tagtcaaaat acagctcctt cgatggtaga ttataatcaa gcttaaaaca atcccaacac
    14581 ttctccgtca tttgtcacgt ccatattcaa tgcagcaggt tttttaggca aaccaggcat
    14641 cgtcataaca tctccagtca aggctactaa aaatcctgcc cctaattttg ggatgatttc
    14701 gcggatcgtg atggtgaaat cttccggtgc acctagcagt gaaggatcat cagaaaatga
    14761 atactgtgtt tttgccatac aaatcggcag tttgtcccat ccattcttcg taaactcttc
    14821 tatttgtttt tgcgcttttt tgctgaaaac aacaccattt cctccgtaaa ttttcttgac
    14881 gattgtttct gtcttttctt gtatcgtttc ttctaggcgg tataatggct tgtaattagc
    14941 ttcttttcta tcaatcaatc gtactacagc ttttgctaaa tcaacgccgc cttctgctcc
    15001 atctgcccat acactcgctc tcttggcaag aatcccctga ttttcacaca agtgctccaa
    15061 cagtgtgagt tcattgtctg tatctgttac gaattcattg attgcgacga tcacaggtag
    15121 ctgatattgt tccatattac gtatatggcg ctttaaattt gcaaaaccta ctttcaaagc
    15181 atcaagattc tcatttttaa gttcatcttt tttcattccg ccatgcattt tcaatgcacg
    15241 gacagtggcg acaatcacca cagcatccgg cgcttttttc aagttaggta ctttgatatc
    15301 taaaaatttc tctccgccaa gatctgcacc aaatcctgcc tctgtgacaa cgtaatctgc
    15361 taatctcaat gctgtcttgg tagctaaaat actattgcag ccatgagcga tattggcaaa
    15421 cggtcccccg tggacaaaag ctggtgttcc gtaaattgtt tgtacaagat tcggcttgat
    15481 tgcatctttc agcagtaatg ccagagcgcc ttctgctttc aagtctccag cagtcacagg
    15541 ctgacggtca aatgtatagc cgacaacgat ccttgcaaga cgagccttta aatcttcaag
    15601 gtctgccgca agacacaaga tagccatgat ttcgctggca actgtgatat caaacccatc
    15661 ttctcttgga acaccttgga ttggcccacc caagccaaca attactttac gcagttcacg
    15721 atcattgaga tcgactaccc gtttccaaat cactcggcgg gcatcgatgt tcaattcatt
    15781 tccttgatga atatgattat ctaaaagagc agataatgca ttattagctg ttgtgatagc
    15841 atgcatgtca ccagtaaaat gcaagttgat atcttccata ggcaatacct gtgcatatcc
    15901 tccgcctgca gcaccacctt tgattcccat cacaggacca agtgagggtt ctcgcaatgc
    15961 aataacagat tttttattga ttttattgag cgcgtctcct aaccctacag tgatcgttga
    16021 ctttccttcg ccagctggtg ttggattgat ggaagtaact aagatcaaat gcccctcagc
    16081 gttatccttt gatcgattga ttccagaaaa attgattttc gccttatatt tcccatacag
    16141 ttccaaatcg tcttccttaa gatctaattt actagccact tcaacaatcg gttctagctc
    16201 tgtttcttgt gaaatttgca gatcattctt catttgatta ccccttttca cgaactttat
    16261 tcaacaaaat atcttctttg ttaagatatg ccaattatac ctttttttat tcagaaaaaa
    16321 aacataaaat tacaggtttt taatttattt gacgtactta taaatactct ttataatgaa
    16381 ggctatgatc agactttaga aaggtcgtga gtgctttgac cacaggaatt gtaaaatggt
    16441 ttgacaacaa aaaaggatac ggctttatca gttatgatga cacagaagaa atttttgtac
    16501 attttactgc gattgaagaa gagggattca aaactttgga agaaaatcaa gtggtcgaat
    16561 ttgagatcat cgaagggaat cgaggaacac aagctgccca tgtcaagaaa ggcaacgttc
    16621 agactaccga tactagtatg catgaataga taaacaaata gagtgagacg ctaaacggga
    16681 tgaaccgtac gtctcactct tttcttttcc tgtaaaaatt cgttttttta ggttctgatt
    16741 tttaaaatta gctttttctt tttttcgtat aaggataagg aattgattct gatttgggtc
    16801 cgtttttttc aaaatgatca aaaaggacta cttgatcttt ttttcgcatc atgaaaacag
    16861 atggggcttt ctcatcttgc aacatcattt gcaccccagt attagttggc gtcaaagatc
    16921 gaatctgtcc gacagatatt tctcggtctt tccaaaaagc agcataacgt ataatcacag
    16981 agtttgattt taaaataaaa gaacgccgca aacctagaaa tagaaaaaca agaaaaatac
    17041 ctaaaacaaa attgcttttc caataaggac ctgtgttttc taaagaaaga atcagactaa
    17101 aaaacagaat gatcaaggtc aatgaccaat agatgatcgt atgtgccagt tcaggctgcc
    17161 atcgaatatt ttttttcatt ctctaacctc tcaccgattc agtaatgata taatcttacc
    17221 ataacattag tttgaacaaa aaggagaatc tctttttatg ttaaaagtat acattgatgc
    17281 ctctacaaaa ggcaaccctg gtcctagtgg aggtggaatc ttgattgtct atcaaggtaa
    17341 acaagaacag ctatctatcc cgctttctat agggacaaac catcaagcag agttcgaagt
    17401 ctttttaaaa actttagaat ttctaaagaa aaacgactta cagaatgaaa cgattttctg
    17461 tttttcagac agcaaaacac tagtttctac ggtcgacaaa aacgcaacga agaacgagct
    17521 cttccttccc taccttcaaa gaatccaaga acttctatcc gaattttctt tattgatcct
    17581 gcaatggttg cccgaaaaca aaaataaagg agcagataat cttgcacgtc aggctctcca
    17641 taaacaatta aaagaaaaaa agactcttta aattaaaaac tttaaggagt ctttttatag
    17701 tttctcattt aatataaaag ataattgtga tcaaacgtaa tttccaattg gtaatctccg
    17761 attttttctt ctatttgact acgtaagatt ctttctagtt ctgtatcaac aaactggaat
    17821 ttctcaggga ccactacatc aaattgcagc gtttttcctt gatgaattac tctaaaatca
    17881 tgaatattaa gccctgtacc tgtttcaaga agaatttgct ttaactgccg caagatatta
    17941 tagtattttt cgttatcaac aggcattgga tccaagtgac agacaagttc cacatccaaa
    18001 gactttttaa agtctttttc tatcagatca ataatcttat gagccacatt caaattcatt
    18061 gattcatcta cttcgatgtg gactgatgca aagcgtttat tgggtccata gttatgcacc
    18121 aataaatcat gatagccaag tatatcatcg caagcattta aatgctcttc catttcttca
    18181 atctctgctg gcgttgggcg acttcctaat agttcgtata caaaatttcg aagcatcgtc
    18241 aatccactat aaaggatata taatgctaaa gcaaaaccta tatatccatc aatacgccat
    18301 ccagttaccc attcgattgc tgctgaaagc aatacagcca gtgtagtaaa gacatcatta
    18361 taactatctg ttgctgtagc tctcaatgtt tcagaatcaa tggattgcgc aataacctga
    18421 tacattttgc cttgtgcaaa tttcaaaaag atggaaatga ttaggacaaa aaagacgatg
    18481 ggtgttaaag agacattttc tggatggatg attttttcag cagaagactc taaaaattga
    18541 aatcctacat aagtaatgat gatcgaaaca aataacccac tgatatattc gaagcgttcg
    18601 tgtccataag gatgttcctg atctgctggc tttgcagcaa ttttaaatcc gatcaaagtc
    18661 aatatggatg aagcagtatc tgatagactg ttcactgcat cagccataat ggagacactt
    18721 cctgaaaaaa gcccgatcat tagcttagag acaaacaaga tcagattcgt aaccaatcct
    18781 aagatacccg ctagtactcc tttttttgtc cgctgttgcg gtatttgttt ctttttttct
    18841 tcttgtttct tagccaagtt ttttacccct tctagtttgc ttgttgctca ggtaaaaaga
    18901 tggagaggac gattgttttc ctctcctctc ttcctttttc tatctatcgt gcgtcctatt
    18961 actattaaaa gatggtcatt attctggttt caacggtgtt gctgcaaata acgcttttgc
    19021 tagtaatggt gttatgattc ccgcacctat tgcttctggt atcccattga ttgctgcaac
    19081 ccctgctaag gttttaaata aaagagcaga gtctacacca taggcatctg ccgttgcacc
    19141 tgtataaaat aatcccatga atcctaacac taagactgta ttgaccaatg aacccagtac
    19201 ccctgcaaag gctgagctga tcgttattga tttatttttt tgataaatcc agtggaaaac
    19261 caagcctgct actaagccaa caagtactct gggaactacc gagataatcg gattcgtgaa
    19321 cactaatgta tcaaatggcg ttgttggcat ggcataggca cgaacaattg tagcgattcc
    19381 ccaaaacaag ccaatcacca tgccatcttt tgggcctaat actactgcag caataattac
    19441 tgtgatatgg ataattgtta aactaatgaa tcctaatgga ataaacccca gccaaggaac
    19501 catcgcttgt acgatgatga ttgctaacaa gatcgcacgt aagactagtc gaaaggtctt
    19561 atttctacta ttcatttttt cacccctttt tatttatact aatcgtaaaa atttctgtca
    19621 ctttcgtcaa ctaaagacca tccgaatccg accatttgat tagatgcgcc atccaattga
    19681 aaaaagtatg agggcaagac ttgtttgtct tcgcccccat aatcatgttt cgttcaatgt
    19741 ttgaactttt gtttttctgt caaacgtaaa cgccctattg ttgcgcaacc cattctttcg
    19801 cagctttaag tgcatctgga ataccagcag gatttttccc gccagcttgt gccatgtctg
    19861 gacgtccacc accgccaccg cctactttcg gtgcgattgc tttaatcaag tcgccagctt
    19921 ttagtccagc ttcatttgat tctttcgtca ttgccgctag cagactgact tttccttctt
    19981 gttcgtttgc taaaacaaga atatctgaaa tatttttttg tttccattgg tcagctaatt
    20041 gacgcagctg attcatatct ttcactttta cttgtgctgc aatgattgtt tttccattga
    20101 tcgtttctgg atctttgaat acatcaccag cttgttgatt cgctaacttg ctagctaatt
    20161 gttcattttc cttttgtagc tctcttactt gctgttgcaa ttgttccgct tttgaaacaa
    20221 cttctttcaa ttgaggtgat ttaacaattg ttgctacttg ttttagttgt ttttcttctt
    20281 cttcaagtag ttcgtacgct tctttacttg ttacagcctc gattcgtcgc acgcctgcac
    20341 caattcctga ttcagaaacg attttgaaga ttccgatgtt ttctgtattt gctacatgtg
    20401 tgcctccgca cagttcgatt gaccagccgc caatatttac tactcgaacg tcatgtccgt
    20461 atttttcacc aaacaaagcc attgcaccca tgtttttggc tgtatcgata tctgtttcaa
    20521 ttgtttcaac tgggatagct tcccagattt tttcgttgac gattctttcc atttcagcta
    20581 attcatctga agtgatttga ccaaagtggg taaagtcgaa tcgtaaatgt ccaggagcga
    20641 ctaaagaacc agcttgattt gcatgctcgc caagtacttc tttcaatgcc ttgtgcagta
    20701 gatgggttgc tgtatggttt ttgatgatcc gattgcgcat cgattgatcg atcaatagat
    20761 gataggtttg tccttcactc attggtgctt ttacttctac ggtgtgcata aattgaccat
    20821 taggcgcttt ttgaacatct gtaacaactg ctacgatttc gcctacttca ttcatgatcc
    20881 atccttgatc cgctacttgc ccacccattt ctgcatagaa aggtgtttga tcaaagatca
    20941 tttgagcttc accttctgcg acttcattca aaagagactc acctttaacg atgaccaata
    21001 attgactatc tgattcttgt tctgtgtatc caatgaattt actttctact ttgatatctg
    21061 tcaataaagc agattgtacc cccattgatt tttcagtact gcgggcagag cgagcacgtt
    21121 cgcgttgtgc atccatttct ttttcaaaac cagcatgatc aactttcagt ccttcatctt
    21181 ctgccacctc ttccgttaat tctaccggga agccatatgt gtcgtatagt ttgaagatgt
    21241 cttttcctgc tagagtcgtc ccgttttcgt tttttacttc ttggatcact tgatttaaga
    21301 tttccaaacc ttcattgatt gtttcattga aacgttcttc ttctgtacgg ataacttttt
    21361 caataaattc tttttgttga agtacttcag gataataact taccatgatt tccccaacaa
    21421 cagggactaa acgatataag aatgcttgtt caatacctaa ttttttccca tgcatgacag
    21481 cacgacgaag caaacggcgt aagacatacc ctctcccttc attcgatggc aatgcaccgt
    21541 cgccaatagc aaatgaaagg gcgcgaatat gatctgcgat tactttgtaa gaaatatctg
    21601 ttactgggtt ttcaccatat tttacttgtt tgcttagctt ttctacggcg tggataatag
    21661 gcataaacaa gtctgtctca aagttcgttg acgcatcttg aacgattgag accatgcgtt
    21721 ccaatcccat ccccgtatca atgtttttat gaggaagtgg ttcaaatgtg ttatcaggct
    21781 tatggttgaa ttctgaaaat actaagttcc agatttctaa ataacgttca ttttctcctc
    21841 ctggatagtt ttcaggatcg tcttctgcta aatcgttata agcttctcca cggtcataga
    21901 aaatttccgt atccggacca catgggcctg caccgatatc ccagaagttg tcttctacat
    21961 caataatgtg atctagtggc aggccaactt cttcatgcca gatgcgtttt gcttctgtat
    22021 cttttggata aactgttaca taaagttttt ctggatcgaa tgccatccat tttggatcag
    22081 tcaaaaattc ccatgcccag tggatggctt catttttgaa atagtcacca atcgaaaaat
    22141 tacccagcat ctcaaacatc gtatggtgcc gtgctgtttt accgacattt tcgatatcat
    22201 ttgttcgaat agatttttgg gcatttgtga ttcttggatt ttctggcaca actgaaccat
    22261 caaaatattt tttcaatgtt gcaacaccag agttgatcca caataaggtt ggatcgttca
    22321 cggggatcaa agacgcacta ggttcaatcg tatgtccttt agattggaag aaatcaagaa
    22381 acatttgtcg aacttgtgca ctacttaatt gtttcatgtc attacctgct ttctataaat
    22441 taaatacaaa aaaacaccat cacagccaag gacgaaaact cgcggtacca ccttgattgc
    22501 aatgatgtgt aatcattacc tcttaaactc gataacgctg agctgcgtcg gtatttctac
    22561 caaaatagaa aaagggagca atttgattag tgactattct tttttcagcc aagaaagaat
    22621 tttctgtgag tcttaaaaaa caaatcatat ccttttcgta cttaattagt atacgcattc
    22681 tattgatcac tgtcaatatc tcaattttta ttttcatttc cttttggtca tactccacga
    22741 tttttggtac gataagaata aaggaatcaa tacatctctg ctattcatga caaggaggaa
    22801 tatgatgaaa aaaatagttg gagcggatga atggatacgc gcagctagct gctttttgag
    22861 atatcaagtt tttgtattag aacaagggat tcctgcagaa ctagaattcg acggaaaaga
    22921 tatcccgcaa caaacctatt atctatttct tgatcaaaaa agaccagtag ctactttgcg
    22981 ttatgaaaag aaaacaccta cttgcttcaa gccggaacga ttttgcgtag caaaagaata
    23041 ccgaggaaaa gggttaggcg gtcagttgct tgaagctgct gaaaaacggg cggtttcaga
    23101 aggttgtcag actagttatg tagtagctga aatgacggct gtagattttt atgaaaaaca
    23161 agggtatcat acttgcacag aaccttttat tgaagatgaa gtactttgta tcggaatgaa
    23221 aaaaagtcta gcttaaaaac aagagattgt gacacgcctt ttgtcacaac ctcttgtttt
    23281 ttatttatgt gaatttttct tatttttacg agcagtacgg ctttgctgtc tgcgttcgat
    23341 tttgcgttct tttttatttt tttctgcaat tgcccaatcg atttttttct tataaccagg
    23401 cttgatcttt ttctttttct tcttcacaag accaatcagc gtgggatcta atacatcttt
    23461 tgatttttct cgtttggcac gtctattacg atcgtatgtt ttgacgattt ccccgttttt
    23521 gatttctttt ggttcaaatg agacgcccaa tttttctatc gcatcaatgg cttgctcatc
    23581 atttggcgaa tataacgtaa tggccgttcc ttttaagcca ttacgtccag tacgtccgac
    23641 acgatggata aagaagtcta gatcttctgg aatttctgca ttaataacat gagagacacc
    23701 ttcgatatcg atccctctag cagctaaatc agtcgctaca acatattgat actccagatt
    23761 ctgtacttgt cgcatcactc gttttctctc tcttggggtg atgtctccat gaatctttgc
    23821 tactttcaag ccttggtttt tcaagtaatc ggcaatttca tccacatgct gttttgtatt
    23881 ggcaaatacg attgctaaat aaggatgacc aattgtcagc agttgataaa tcatttgatt
    23941 ctcatcttta ccttttgttg aaatcagcca attttcaatg tcttcagaaa taactgcttt
    24001 tggtttgatt tcttcaatga ttggattttc catgtatttt cgtaagaatg gacgaagttt
    24061 ttctggaata gtggcagaaa aaactaaaaa ctgcagcttt tctggtaatc gacttgcaat
    24121 ttggtctact tcatttaaaa aacccatatc caatgtcata tccgcttcat cgacaacaaa
    24181 tgcaaatgcg gtgtgaatct tcagcgcttg ttcattcatc atgtctaata ttctaccagg
    24241 cgtgcctact acaacctgtg gctgttggtg atgcagacga tttaattgac gctgtttgtc
    24301 tgtaccgccg acaaaattcg agacacggat ctccggtgta gaaaatttag cgatttgtgt
    24361 agctgcttca tagatctgtg cagctagttc cctacttgga gctgtaataa caacttgtac
    24421 ttcctctttt tctgcattga ttttttcaaa caaaggcaac aaaaacgtat gtgtttttcc
    24481 tgtaccagtt tgagattgcc caatgatatt ttttcctttt gcaattaaag gaatcaaacg
    24541 ttcctggacc tctgtgggct gtttgaagcc ctttgcggtt aacgcttcat tgataaacgg
    24601 tttgaagcgg aatatatcaa aattattcat tcgtgcacct cttctcttta aattatcacg
    24661 actttggcat tataacacag acaaagcgtt tttgctcagc taatttcgag aatcatcatc
    24721 ctttgtttgg ccaatcgtgc taacagtata tgtgtgacta gctttttcat aaaacagaat
    24781 ccgttttttt ctcaaaaatg atagcaagat atttaaaata aaaatacctt gtagtaacaa
    24841 gacaccgaga gaaatgaata ctgcactcat ctgcaacacc gtattcgttg tcgaattcat
    24901 gatttgctga agcaactgcc ccaaccagct taaaccataa aaaagaaagt acagataccc
    24961 gtagcggatc atcaatgctt ttaaagaaat tcgttcgcgg tcttcttcca ccaaacggat
    25021 acgcacaatt tttttgccaa gtgtccatcc attggttata aacggtacaa tcataaagta
    25081 tacccctact tctaataaaa gccaccagat atgagaagta actgcccctg aatcgaacgc
    25141 aattttaaag aaaatcgccg ctaccaaatt aataagagat aaaaatgtcc agtcgatcaa
    25201 ataagcgacc aagcgtcgag tcaatgagac agtctttcct ttttcataag aaagttcatc
    25261 cattcgtctt cttgtaggaa gcataaatgt aaataacggc gtaacgacaa aaccaatgat
    25321 tccacccaat gtatttgtca ctaaatcatt cacatcaaat aagcggtatg gtcttgggta
    25381 gataaaataa agtccactta gctgcgtcaa ttcaaaaaat agagaaaggc agaaactagc
    25441 aatcatcgtt ttcataaaag aaagccggaa ataatatctt aaataaatgc caaacggtat
    25501 gagtaataaa acattaaaag ctggttccaa aaatacactc tgcttcattg ccggcaggta
    25561 ggtactaggt tcagacaaga ccaataccgt gttgtctctg aaatgttgcc atgaagcaaa
    25621 taaatgcagc tccatctttg gccctgtcat ttgggcaacc gtctcctttg ccggaagtgg
    25681 taagatcacc aaaaaatacg cacacaataa ataaaaaacg aaagaataca aaataactgc
    25741 ccttgttact agaaatgttc catactttcg atattcataa ataaaaaaaa cagatgagat
    25801 cgctaaggct aaaaatggaa aaacaatcag cgcaaaacga atcggttcga tataacttgc
    25861 catatgactc ctccttgatt ctttgtatct ctcttatctt agcagtcctt tgctgtttca
    25921 agcaagtaac gaggctatta tttctcattt ctttcaaagt aaagtaagat aaacacaaag
    25981 ggggtgtcga gatgctgaat atgtactttg tttttggcgt acctattttt ttactgttcc
    26041 tttatgctac gatcgcttat gtaagaaaaa gaactacaat tcattattta ggatttattc
    26101 tcctgattat ttcgggtttc atgctagtct tcaatcttca gacttggcag caagcacttc
    26161 ttgaaatgga taaaatgact ccacatgctt tgtctaaagt gcttggctac cctgtctatc
    26221 tgatttggct tcccattttc atttctggtt gcttggtttt attaaatatt tatcgcggcg
    26281 tacgccgaat agttcagtta cgcaaatcaa agtaaaagac agaaatcggt tgagcccgat
    26341 ttctgtcttt tactttgcta tttcatcgta gacaaaacga catttacgtg atcaaaatta
    26401 acgagtactt ttgacatttc ttccacgtac tcctcattta aataaaatat tttgtttggc
    26461 agagaagtgc agttctaata aaattcgaat gttttagcta tctacgtttt aaatagagat
    26521 aaatcgggat accagcaagt gtcaatacta tccccatcga agctaaaaag gtctgagtga
    26581 taagcgtgct tgatacgata aaagtccctc caatgattgc tactagtgga ataaacggat
    26641 acattggaac tttgtatggt cgaaacaaat caggttcttt tttacgaaga agaatgacac
    26701 caacaaagac catcgtataa aaaatccaaa tgacaaagat cagcatatcc gttagtgtat
    26761 caaaaccacc gatcatcatc atgccaatag cgatgactag ctctaagata cctgcggcta
    26821 caggaacttt agttttatca tgaagtttag caaagagctt gctaaaaggc aggttgtttt
    26881 cttttgccat aacataaggg agacgcattc cagtaagggt atagccattg atggttccat
    26941 aaacagaaat caagatacca actgtgacca gtcttccacc gaagccgcca aaaatcattt
    27001 ttgcaacgtc acttgccgca ttgctgtttc ctgcgactcc gtcgatagat gcggttctta
    27061 agaacaccgc atttactaat aaataaacga tcatgattcc gatgattcct aaagaaattg
    27121 cttttggcaa atctttcgcc ggctttttca attctcccga aatatttcct acgtgtatcc
    27181 aaccgtcgta tgcgaacatc gttgctagca agcccgctcc caaggctgag aaaaatgaaa
    27241 gattttctcc tgcttggatc ggaaacagtt ggaagtctac tccttcttgt cggaacaaac
    27301 cgaaaatgac gatcacaaac aaaggaataa gcttgcagac aagcgtaatc gactgaaaag
    27361 cccctccagc tttagaacct aaaaaattga tcaacaggat cgatacagca gctgtaacag
    27421 ctacgggaac aatcattgac tgggaaagac caaatagatt gacgaactgt gtaccgaaaa
    27481 tgatcgacaa ggctgctaca tttgctggaa aataaatcac gacttgcgcc cagcccaata
    27541 aaaaagctgc tgtatttccg taaatccgtt cgatatattt aatcatccct cctgtttctg
    27601 gaatagcagc tgccagttca gctcctgtta gtcctgcaca gacagaaatc ataccaccga
    27661 gaaaccaaga aaacatatgc aaactggcgg atcctgtaac ttctgctacg cttgcggctt
    27721 taaaaaatac ccctgcaccg atcactgtgc ccataacagt cgataaagca gtaaaaaatc
    27781 ccatcgtacg ttttaattca ttttctattt ttacttcttt tactggcgtg gcttgatatt
    27841 cttccatccc ttgtcccctt tcataccata gccaatccca aaagtataaa aaactttttg
    27901 gatgtggttc ttgttttata ccgtctctac caatttttct ttcagttttt ccaacatatc
    27961 ctttgtcatc acatctaagt cgtatttcgg cgaaaagccc cattcctctt gcgcacaaga
    28021 aacatctagt gaatttggcc aagattctgc tattgactga cgtacaggat ccacatcata
    28081 atccatttca aaatctggta tgattttttg aatagatgct ttgatctctt ccgggtcaaa
    28141 agacatcgcc gatatattga aggcatttcg atgaaccaat cgatcaggat ctgcattcat
    28201 gagttttata atggcatcga tcgcatctgg catatacatc atatccattg atgtaccttg
    28261 atcaatgaaa ctagtgtact ttccttcttt gattgctgaa taataaatat cgactgcgta
    28321 gtctgtcgta ccgcctcctg gcaaggttgc ataagaaatc aatccaggga aacggacacc
    28381 acgagtatct acgccatatt tcacataata ataatcacat agtagttctc cagctacctt
    28441 cgttacccca tacatggttg taggacgctg aagagtatct tgtggggtat gatcttttgg
    28501 agtatttgga ccaaacgcgc cgattgaact tggcgtaaaa aatttcagct ggaattctct
    28561 agcgacttcc aatgcattga ccagcccagt cacgttcaaa ttccaggcta attgtggttt
    28621 tgcttctgca acagctgaca aaagagcagc caaatgaatc aatgtgtcca cttgatattg
    28681 ttctgctatg ctgcgcattt tttcgtaatc cattacatct aggatctcaa agatatcgtt
    28741 tttctcaatg acttcaggca tgcgtatatc tgtcgcaatg acattttcac tgcctagttc
    28801 ttgacgtagc cgtgtgatca actctgaacc aatttgtcct aaacatcctg tcaccaatac
    28861 ttttttcatg atgatcctcc ctgatctttt ttactactat cttcagatga ttaacaatat
    28921 atgcgtgaac atgttctcta attttaaata gacgaaaaaa ctaaatttag ttaaataatt
    28981 tccatttctt ttcctatttt ttgataaact gctactgctt catcaagcat ttccctcgta
    29041 tgttccgcag tcggcatatt tctgatcctg cctgtcccaa gaggcacagt tggataaacg
    29101 attggtttag cataaatccc atgttcgatc agcttcttag agaaagtctg agtcactttt
    29161 tcgtctccca aaatgactgg agtaataggt gtttcagaca taccgatgtt atagcctact
    29221 tttttcaatt cttctttaaa ataattagca ttttcccaaa ctttttcgac aagttccgga
    29281 tgttcttgca tcaaagtaat cgaagccaat gcagctgcag ctgcacctgg agttagcgaa
    29341 gtagaaaata ggaatggccg acttctagct ttcaaccagt cgattagtgt tttcgatcca
    29401 gctacataac ccccaacaac acctatagct ttcgataacg tgcccatttg catatcgatt
    29461 ttgtcgctta atccaaaatg cttgacggtt cctgcacctt ttccagttac gcctgagccg
    29521 tgcgcatcat cgacgtaggt gatcagtcca tatttttcaa caatcggaat aatttccggc
    29581 aatcgtgcaa tgtctccatc catggagaaa acaccatctg tgatatacat gattttttta
    29641 tatttcttac tttctatcgc ttcctttgct tttttctcaa ggtctttcat atcttgatgt
    29701 ttaattcgga tgatttttgc tccggataag cggcaaccgt caataataga tgcatggttt
    29761 aattcatccg ataagatagc atcttctttt gtcattactg cagagatagc gcccatattg
    29821 cagttgaatc cagattgaaa ggcgattgct gcttctgtcc ctttgaattc tgcgattttt
    29881 tcttctaatt gctgatggat tttcagcgaa ccattgatcg ttcggactgc tcctgctcct
    29941 acaccataag tttcggttgc ttcaatacat gcttttttca attcttcgcg attggcaaat
    30001 ccaagataat tgttggatgc tagattgatc atcttttttc cgtctacgat gatacaagca
    30061 ccattttcac tttctagtat gtcgatcgta ttgtataacc ccttttcttt cagttgatcc
    30121 aattctttct ctaaatacat tgttagttct ttggaagaca ttgtctccac ctccatattc
    30181 atctgtcttg cagaacatac caaacatata tcgtgtcatt catctacaaa gcaagtatgc
    30241 tatatttttg ataacgctta cactgattat gagaattatt taaccacatt ttttatttac
    30301 agtatctttt tactttcaaa tgacttatat agtatgaaca attacctttt agataatgat
    30361 ctattttact ctacctgggc agataaatat aacacaacca ttgatagaag
//
LOCUS       DAZBSA010000024        29445 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000024 DAZBSA010000000
VERSION     DAZBSA010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.024"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(147..1478)
                     /locus_tag="ACHGMT_000987"
     CDS             complement(147..1478)
                     /locus_tag="ACHGMT_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFN6834753.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(1627..2340)
                     /locus_tag="ACHGMT_000988"
     CDS             complement(1627..2340)
                     /locus_tag="ACHGMT_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN6834754.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            2515..3939
                     /locus_tag="ACHGMT_000989"
     CDS             2515..3939
                     /locus_tag="ACHGMT_000989"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN6834755.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            4238..4945
                     /locus_tag="ACHGMT_000990"
     CDS             4238..4945
                     /locus_tag="ACHGMT_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFN6834756.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            4932..5255
                     /locus_tag="ACHGMT_000991"
     CDS             4932..5255
                     /locus_tag="ACHGMT_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFN6834757.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(5344..6381)
                     /locus_tag="ACHGMT_000992"
     CDS             complement(5344..6381)
                     /locus_tag="ACHGMT_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFN6834758.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(6702..7685)
                     /locus_tag="ACHGMT_000993"
     CDS             complement(6702..7685)
                     /locus_tag="ACHGMT_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFN6834759.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(8100..8870)
                     /locus_tag="ACHGMT_000994"
     CDS             complement(8100..8870)
                     /locus_tag="ACHGMT_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6834760.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(8863..9798)
                     /locus_tag="ACHGMT_000995"
     CDS             complement(8863..9798)
                     /locus_tag="ACHGMT_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6834761.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(10114..11763)
                     /locus_tag="ACHGMT_000996"
     CDS             complement(10114..11763)
                     /locus_tag="ACHGMT_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFN6834762.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(11880..14483)
                     /locus_tag="ACHGMT_000997"
     CDS             complement(11880..14483)
                     /locus_tag="ACHGMT_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFN6834763.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(14495..15544)
                     /locus_tag="ACHGMT_000998"
     CDS             complement(14495..15544)
                     /locus_tag="ACHGMT_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFN6834764.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(15570..15884)
                     /locus_tag="ACHGMT_000999"
     CDS             complement(15570..15884)
                     /locus_tag="ACHGMT_000999"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFN6834765.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(15900..16346)
                     /locus_tag="ACHGMT_001000"
     CDS             complement(15900..16346)
                     /locus_tag="ACHGMT_001000"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFN6834766.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(16347..18269)
                     /locus_tag="ACHGMT_001001"
     CDS             complement(16347..18269)
                     /locus_tag="ACHGMT_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN6834767.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(18481..19377)
                     /locus_tag="ACHGMT_001002"
     CDS             complement(18481..19377)
                     /locus_tag="ACHGMT_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN6834768.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(19370..20050)
                     /locus_tag="ACHGMT_001003"
     CDS             complement(19370..20050)
                     /locus_tag="ACHGMT_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN6834769.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(20051..20833)
                     /locus_tag="ACHGMT_001004"
     CDS             complement(20051..20833)
                     /locus_tag="ACHGMT_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFN6834770.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(20826..21743)
                     /locus_tag="ACHGMT_001005"
     CDS             complement(20826..21743)
                     /locus_tag="ACHGMT_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFN6834771.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(21907..22161)
                     /locus_tag="ACHGMT_001006"
     CDS             complement(21907..22161)
                     /locus_tag="ACHGMT_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834772.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
     gene            23018..26245
                     /gene="fss3"
                     /locus_tag="ACHGMT_001007"
     CDS             23018..26245
                     /gene="fss3"
                     /locus_tag="ACHGMT_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFN6834773.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            26358..26672
                     /locus_tag="ACHGMT_001008"
     CDS             26358..26672
                     /locus_tag="ACHGMT_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFN6834774.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            26685..27059
                     /locus_tag="ACHGMT_001009"
     CDS             26685..27059
                     /locus_tag="ACHGMT_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFN6834775.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            27069..27404
                     /locus_tag="ACHGMT_001010"
     CDS             27069..27404
                     /locus_tag="ACHGMT_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFN6834776.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            27487..28827
                     /locus_tag="ACHGMT_001011"
     CDS             27487..28827
                     /locus_tag="ACHGMT_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFN6834777.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            28905..>29445
                     /locus_tag="ACHGMT_001012"
     CDS             28905..>29445
                     /locus_tag="ACHGMT_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFN6834778.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHP"
BASE COUNT         9065 a         5763 c         5218 g         9399 t
ORIGIN      
        1 cttgtgaaaa ataatgatta tccttaccaa aaaatgtttg tttttctaca tgttcgtact
       61 tatcaaaaaa tatgatcctt attgaaatta ctggctattt atattccaac gacttcttgt
      121 gttaattttt gttatctcat agtaaattat gtttcatttt cacttaattt agttgtcacg
      181 tgttcgtcaa ttcgtaaaaa cggtaaatat ataaacacac ttgcggctat aattaatatt
      241 tgtaaaacag ctgctcgcca gtctccggct gttgctaaaa atcccgatat aactggtggc
      301 gtagtccaag gaatttgcac tactacatga ttaattaatc ccacagcagt tgcaaaatat
      361 gctactgtca aagaaaaaat cggtgcaagc acaaagggaa taatcagact gatattaaat
      421 acaataggca atccaaaaat aataggttcg cttatattga atatagcggg agttaccgat
      481 aatttagcaa agtccttata atcttttcta cgactgaaga taaatattgc aatcaataaa
      541 gcaatcgtat ttcccgatcc acccattacc gcaaaggtat ctttaaaggc catattgata
      601 atatgtggaa tttgctcttt gtttgcataa gctgccatat tttcttgcat attctgaatc
      661 aaaaacggat ctaacaatga acctgatatt actgcttgat ggataccaaa tccaaaaaat
      721 agattagcaa tagatgtaat taacaaaaat cctggtaaac cagttgtaat tttacttaat
      781 ggctctgtaa ctatcgtggt aattagctta tttatatcca tattaaacaa ttgaatgatt
      841 ataaatgaca ataaagcaaa cgaggtaatc gtaataatga ttgggattaa tacattaaaa
      901 gactttaaaa cagcgggagg aatatttccg gatagctgaa tctgtagttt cttattatta
      961 gaaagtttaa taaataattc tgtagccgct aatccaacaa atatacctac aaacattcct
     1021 gaagctcctg tataggatat aggtatcaca cccgtcactt caacagcttt atccgcattt
     1081 tcaggagtaa acatcatctg cataggtgtt aatacaacaa atgtcgaaat agcaactaat
     1141 accgctgcaa tatgatttgt ataatttctg cttgcacaca tatgataaga tactgctgct
     1201 acaactaata atgtagttat gcttaatgta ccattggcaa ttgaagtacc aatttgttgt
     1261 aacgtggtca atgtactagt atcgacaatt tcccccatga atcctgttgg ttctaagatc
     1321 actgaattaa taagtgtcac aaatcctgct aacatcataa atggcataaa tgtagcaaac
     1381 gcatctcgca aagttcttaa atggatctga ttccccaact tagtcgcaac tataccaaat
     1441 ttttcaaata caatgtcctt cctgctactt ttgcccatat tttttctcct ttatgcttat
     1501 tgtaatcgat tactttttcc acccaaataa tacatcactt atcaaaaaaa gtaaatactt
     1561 ttaattaaat atatatatct tttttagtat aaaaaaataa gctctgaaaa aataattttc
     1621 ccgagcttat ttttcaacaa aaacagcttc aaaaacaaag tcttgatata aatgtctcga
     1681 taccgaatac tcgaaaggtt taccagacga tagagcaaca actttgtcta tctcaatcat
     1741 aaattcacct ggatctattt ttaatatttt tgcgtcaaat tcattcgcag tatctccttt
     1801 gatacgtatg tgcgaagtct ttatttccag acccagttca tttgtgatat aagcatatac
     1861 agatttcttt aaatgttttg gttccaagcc tggtatgact gctaatggca tgaatgtttg
     1921 ctcaatcgag tatggttgac aatcaataaa acggcttcgt acaatattat atacatagtc
     1981 ttttacactg atacttaatt tatcagctac ttttttcgat ggaagttcaa ttgagaaagt
     2041 atggatttct gaatctacgt gttcttcacc aaccacgcta aacaaacctt cgtttggacc
     2101 aagaactcgt gagttatcaa ttggtgttct gacgacttca gtcccatacc cacttctact
     2161 tcgcaaaact ccttcggaaa caagtaagtc tacgcctttc tttactgtta acttgcttgt
     2221 atgataaata actgccaatt catctcctgt aggaagtttc tcaccaattt tatactctcc
     2281 atttacaatt tttttcttta tatctaagta aatttccata tacttcgcaa tttttttcaa
     2341 tggtgaccct ccaatcaaaa acatatcttc atctatactt taacataaat aaaccgataa
     2401 aatccacatc aaaattatac acacaataaa aaagatatat atatttaaac aaaactattt
     2461 actttaagtt gtaagcggtg ttatcattaa gtggaaacag gaggtaaaaa aattatgaca
     2521 actatgaaaa agctaacttt tccagatgat ttttggtggg gttccgcttg gtcagctgag
     2581 caagcagaag gaaaaggaga aacaggaaaa gcagagacta tctgggatcg ctggtttaaa
     2641 gaacaacctt accggtttta tcaacgaatc gattcctcta ttacaaccga tcacatccat
     2701 caatataaag aagatataga actaatgaaa aaaactgggc ataactcctt tcgcgtttct
     2761 atttcttggg cacgaatgtt tccaaatggc ggtaccggaa aagtaaatcc caaagccatt
     2821 tctttctatc gaaaattatt ttccgaaatg aataataacg gaatcaaagt atttgccaac
     2881 ttatatcatt ttgacatgcc tgctactctt caagataaag gaggatggga atcacgtgaa
     2941 gtagttgatg cttatgttca ttacgctgaa acttgtttta aagaatttgg tgacttagtt
     3001 tgtcattggt ttaccttcaa tgaaccactt gggcctattt taggtactta tcttgaagat
     3061 tttcattatc caaacctcat tgactttaaa cgcggagcgc aagcagcatt cttcactata
     3121 cttgcacacg caaaagcaat acaagtattt aaagaactag ctcttcctag taaaataggc
     3181 gttattctaa atttgagtcc tacttttcct cgtagtcaaa atcctgctga tgtgaaagca
     3241 gcagaaatcg ctgatttatt ttatacgcgg agctttttag atcctatggt taaagggaat
     3301 ttttctaaaa agcttatcct gttattacaa gaatataatc agcttcctga agattatacg
     3361 aaagaagatc ttgtatgtat ctacgaaaat accgctcaaa ttcttggact aaactattac
     3421 gagccacgtc gcgtcaaatc tcgttcttct atggtggata catctagtcc atttttacca
     3481 gagtggtttt tcgaaccaca tatcatgcct ggacgacgaa tgaacgaata ccggggatgg
     3541 gaaatttacg aaaaaggaat ttatgatttg tgcatggata tcaagaacca ttatggaaac
     3601 atcgaagcat ttatctcgga gaacggaatg ggcgttgcgg atgaagaacg tttcatggat
     3661 gatacagggc aaataattga tgattatcgg attgagtata tcaaagatca tcttgcttac
     3721 ttatggaagg caataaatga aggatgtaac attaaggggt atcatctttg gacatttatt
     3781 gattgctggt catggatcaa cgcctataaa aaccgatatg ggctggtttc actcgacctt
     3841 actactcaaa aaagaacaat taaaaaaagt ggcgaatttt acaagcaatt aagtgataca
     3901 aatggattta gatacgatgt agacttgtta gttgaataat attctaaaat tccacttgaa
     3961 gtgaacacat tagaaaacaa gaataatgag aaataccaat aacaaatact ttttcttcat
     4021 acttcattta tacttgtcag tataagagac tgtgacaaat gtagtgatcc tcagaagtta
     4081 ggtctggaaa tctaacttga gaagattttt ttaaactacg tccagtcttt tttatttgtt
     4141 taaaattgaa gttgacaagt cttcattaaa aatttagaat attcacaagt ttgacgacaa
     4201 ttatgaaaat ttatgtcgtg ctaaaggagt tttgttattg gatgaaaagt tagatataaa
     4261 aactgcgatc aaagacacgt tacctaccgt ttttggttat atcggtattg gacttgcatt
     4321 tggtatcgtt gggaaagctg ccgggtttca tccgctggtc gtcacgttga tgtccctgct
     4381 gatctatgct ggttctgccc aatttatcac ggtcagcatg cttgctagcc atagtccact
     4441 gctctccatc gttctctcga cctttctagt caattcccga atgattctga tgagtatgac
     4501 gattgctcct tatttcaaga aaaatcggct gcttcaaaat ctgctgattg gcacattgct
     4561 gacagatgaa agtttcgcac tgggaatgaa taaattgaac tatactggac aaaagttaaa
     4621 ctttcgttgg atgaatacag ctaattggat ttcttatttg acgtgggtgg gttcttctct
     4681 tgttggcgca cttttaggga attttattac tgatccgaaa aagtttggtt tggatttcgc
     4741 catcgtcgct atgtttatcg ggttacttta tcttcaagtc atttctgata gaaacacctc
     4801 taaacgatta cagttgatcc tgattggtct cactttgatt ctggtatatg tcggactgat
     4861 ttttatccca agtaacttag tgattttagt tgtaacactg attgggtgcg gattgggggt
     4921 gtggatcaaa catgccttct tttgagttta cattgctgac tattttaggt tgtactttag
     4981 caacttggct ttctaggatc ctaccatttg tcttactgaa aaaattcgat ttgccgcaac
     5041 cgttgcttga gtatttaagt tttgtcccga tcgtcattat gtctgcttta tggttcagca
     5101 gtctatttac ccaaaatatc ggacatctgc cgcagatcaa tctcgaaaat gctttggctt
     5161 cagttcctac acttttagct gctattcttt caaaaagctt attggtgatc gtcttggtag
     5221 gaattctttc cctttcgttg attcgcttat tataaataaa aaaacaagag cttatggcaa
     5281 attactgtca tgcgctcttg tttttatcaa agtcaaataa gaaaaaaatc ggcgtattct
     5341 tgcttatccg ttgaacggac cactaacaac gacttttcct aatatatgtc ctgtttctac
     5401 gtctttggtt gcttgtttca agttcttcgc attcatccca tctgaataga ccttgctcaa
     5461 tgtactgcgg ataatccctt catttgctaa agcagcaatg tgcgctaaag cttctccttg
     5521 cgtttcgatt tcatagttat agtctgtttt agcgaacata tactcccagt cgaagctgat
     5581 agattggttt ttccaaacac ctatatctaa cggctctttg atccctacga tcgttcccac
     5641 atgtccaaat ggtcggacaa ggtgtttgat ttgatcaaag tagcgggtaa tatcgaataa
     5701 tacagcgaca tactctactt gatcgattcc taattcacga aaacttttac ttaagtcgtg
     5761 atgataatcg attggatagt ctacacctgt ttttttcagc cattcaaaat tcttcggact
     5821 tgcagtcgcg tagacttcta ggccagccca atgcgctagt tgattcaaaa tcgaaccgac
     5881 gcccccagct ccgttgatca ctaagatttt tttcccgcgg ttggcatttt cttctggagt
     5941 cagaccaaat ttttcaaata acaattcata agcggtcaat gaggtcaacg gcaaggctgc
     6001 ggcttcttca tctgaaagat tttttggtgc aagagcgaca atacgttcgt caaccagttg
     6061 gtattcttga ttgcttcctg ctcttgtcgt tgtacctgcg taaaaaacgc gatcgccgac
     6121 agaaaatttt tgcacttcat cacctacagc aacaactttc ccgactccat caaaccctaa
     6181 gatacgtaaa gcatttctta tcccgttatc ttggcgaagt ttcgtatcga caggattaac
     6241 agatactgcg ttgactttta ccagaagatc ccgtgaactg ggtacaggta tctgttcttt
     6301 ttcatcaata aagctttttg gatcatcgat cgacaaccct tcataaaaac cgactacctt
     6361 cattaattcc ttttctgaca tacgaattcc tccttagctt catcaagttg gagcatccat
     6421 acagttttca tttcaatata tatagtatgc tatttgttcc acttttattc atcataatta
     6481 ttctcttact taaagtaaac tatttgcact tttttggtta aaaaagaggt tgcgacaaga
     6541 cttttgtcac aacctctttt aaacggtgtt caatcaactg accctcggta ttcgctcaaa
     6601 aaacgacaaa atatgaggag ctatttttgt ttggctgttc ttattacttg atgcaggaca
     6661 gctttttcac gacctctaat tcacggtgtt caagcaagaa atcattgttc actgaaaagc
     6721 gttggatgtt ctttttcaaa ggtgtctaag atctctcgta cttcatcagt agatttcgta
     6781 ttcatcaact gaactcgcaa gtcatttgct cctggaaatc ctttgatata gattttaaag
     6841 aaacgatgta gtcctgtgat ggaacgcggg atgatttctg aataatgatc ttgcagatct
     6901 agttgcagac gaagtagtcc gatcagttct ttttctgtat gttccctcgg ttctttttca
     6961 aatgcataag gattcttgaa aatcccccgg ccaatcatga tgccatctac gccatatttt
     7021 tcggccaatt cttctcctgt ctggcgatct gggatatctc cgttgatcgt gatcagtgtt
     7081 tgcggagcga tttggtcacg taaagcaatg attttaggga tgacttccca atgtgcatct
     7141 actttactca tctctttacg cgtacgcaaa tggacagaaa gattggctat atcttgatga
     7201 agtaggtgac tgatccattc ttccatctca gacatttccg tatagccgat acgcgtcttg
     7261 acgctgacag gcaaaccgcc agctttagcc gcttcaatca attgtgccgc tacttctggg
     7321 cgaaggatca agccactgcc ttttccacgt tctgctacat tagggactgg gcagcccatg
     7381 ttgatatcga ttccttggaa gcccatttct gccatagcaa tgctcatttc tctaaaaaat
     7441 tctggcttgt ctccccaaat atgggcaacc atcggctgtt catcttctgt aaaggtcaat
     7501 cttcctcgta cactgtcttt tccatctgga tgacagaaac tgtctgagtt tgtaaactca
     7561 gtaaagaaga catccggtgc tgctgcatgc tttacgacat ggcgaaatac aacatctgtt
     7621 acatcttcca ttggtgctaa aacaaaaaaa ggctttggca gctctttcca aaaattagta
     7681 gtcatttatt tgttactctc cattctatca agcagtgaag tcgcaatctt tctcaaaaac
     7741 attgtcatta tactaggaaa caaaaaaaga agcaaagtga acagcttaaa agatgtttct
     7801 gattgagcaa aaaaagactg tgacaaaaat cttatcacaa ttccttttcc ggaataaatg
     7861 gtgttcaaaa gcttgcatct gcggtaataa gtccaaccaa acgaaaaata ggaggagcta
     7921 tttttgtttg gctgttctta ttacttgatg cagaacagct ttttcacaac ctctccacta
     7981 aacggtgttc aatcagctga ccctcggtat tagctcaaca aacgacaaaa tatgaggaac
     8041 tattttcccg tttgtttctc taatactcag gtcaaaacgc tgatttcaca acctcttttc
     8101 taacttctca atcctcttcc tttattgatc aaataccaag taacggcgta caaaacaacg
     8161 atgaatagca caagtacgat catcgatgtt gctactggca catcgattgt tccaaggaat
     8221 ccgtaacgga aaccggagat catataaacg atcggattga ctttcgaaac agcttgccaa
     8281 aatggcggca acatagagat ggcatagaaa acgccaccta gataagttaa cggctgcaag
     8341 acaaatgtag gcacgatcga tacatcgtca taagactgag caaaaatccc gttgagcagt
     8401 cctgctaacg aaaacaagat tgccgtcatc aataatgtaa tgatgaccat gaaccatgaa
     8461 tacacatgaa gtggtacaaa gaacaaggaa atgatcgtta cgagagagcc gacgagaata
     8521 ctgcgaccca ctccgccaat cacaaagccc caaatgatca catgcgtagg aacaggcgct
     8581 accaagattt cttcgatgtt tttctggaat ttttgagaga agaaggacga agaaacgttg
     8641 gcatacgaac tagtgatagc agacatcata atcaaaccag gcacgatgaa ttccatatat
     8701 gaaaaaccac ccatgtcacc gatccgacca ccgatcattt ttccgaaaat aacaaagtac
     8761 aaggatgtcg tgatcacagg cggaaccagt gtttggaccc agatacgcaa atagcggttc
     8821 gtttccttga tagctaaact ttttaatgca gtaaaataaa gattaaacat ttttctcctc
     8881 cgtatgatta ttttcttctg tgattttcaa aaacagttct tctaaacgat tcgacttatt
     8941 cctcatagaa agaactttga ttccttgtct tgtcagctga tcgaagattc cgttcacccc
     9001 ttgatttcgt tcaacttcga cagcgattgt ctgttcatct tcaaatgtac aagcgtatcc
     9061 ttcaatcaca ggtttggtct catatggcgc taaatcaaag ataaatgttt caaattgcag
     9121 tttggataga agctctttca tactagtatt ctcgatcagt tcacctgatt ggatgatccc
     9181 gatgttgcga caaagcattt cagcttcttc taaataatgc gtcgtcaaaa tgatcgttgt
     9241 accattttcg ttcaattctc ggagaaactc ccacatctcg cgtctcaatt caatatcgac
     9301 tcctgctgtt ggttcatcca agatcagcaa acgtggttca tgcatcaatg ccctagcaat
     9361 catcagtctt cgcttcattc ccccggaaag catacgtgca cggacatttc tcttttccca
     9421 aagattggac tgcttcaaat atttttcgct gcgtttcatc gcttctttgc gagagacacc
     9481 gtagtagcct gcttgattca cgacgatttg ctggaccgtt tcaaagggat tgaaattgaa
     9541 ctcttgcggg actaatccaa tctgttgctt tgctcggact agatccgtat ccagatcata
     9601 accgaagaca ttcacttttc cagatgtttt attgactaaa gaagtaatga ttccgatcgt
     9661 tgtagacttc ccagctccat ttggtcctaa tagcgcatag aaatctcctt ctttcacgga
     9721 aagatcgatt cctcgcaatg cttcgacccc acttccataa acctttttca aattatttat
     9781 ttcaagcgca tttgccattt atttcctatc tccttataga catgattctt tattgtatca
     9841 tggaatacag cagatgaaaa aacaacttgg ttccagcgaa aaatcaggtt gctgggctca
     9901 ggaaaatctc ctaatgaaag acatcaatcg gacgaagtca gacaaccttt tcagaaaaaa
     9961 caaatcgaag ataaaggatt actattcata tgtgcaacaa aaggagcgtg agacaaaagt
    10021 aaagaacact tttgtctcac gctctaaaca cgtataaacg gcggaagcaa aagccaagat
    10081 ttttgatcca ataaatactt tcgatcactt cgcctatccc aatacatgcc actgccgctt
    10141 ttcatcaaat aacgcggctt ccgtttttgc ggtttctact aaagtctctg ggtaatccaa
    10201 aactttcagc aaggcaatcg catttcgggt ccttgaaggt ccttctttta atttgaaatc
    10261 aaaactgatt ccttttcctt ctgttacttg ttcttcaaag tggacattct cacaataatt
    10321 ctttaaaatc tctgtcaatt cgatatcatg tgtggcgaca aatgctaaag atggatactc
    10381 cgcaagccac cgaacgactg aagcggaaga agcaatccgt tcgatcgtat ttgtcccttt
    10441 taaaatctca tctacgaagc agtagcagcg ttctttggaa gccacttggt ccaacaaacg
    10501 tttgatcgat ttgatttctg aaacaaaata actgtcgcct tcaaataaat catcttcgat
    10561 tgccatggaa gtcaacacat gtccaggttg cagggtaaac tgttctgcaa tcgctgtttg
    10621 gatcgtctgt gctagaatac agctgatggc tatacttttg acatacgtag attttcctga
    10681 tgcattggaa cccgtgacta atgtattctt ctgccagttg accggattca ctaccgcttc
    10741 ttttaacaat ggatgataga tatctttcgc tgtcacaccg ccctcagtaa actctggtcg
    10801 gcaagtgatg ggcatataag tacggtaatt caatattgca gcggctactt ctaattctcc
    10861 cagcaattcc cacagcttga tggcttcagt tgaatgcttt tctaatttag tgagaacgaa
    10921 atgataggag ataaatggca gcatgaacat gatgttgaca tattcaaaaa tcaactctgc
    10981 ttcactccca ctcttcatac gaaatgaaaa acctaattta gggatgtctc ttaatggttt
    11041 agtttgctgt ttcagctctt cttgaagtgg tgtcggcagt tttgctagct gattggcggc
    11101 tgaaatcgtt gaaaccaaat aactcatgct attcagctct gtttctagtg tttgtttctt
    11161 cactagataa tagaccgtat tgaatactaa cccaccgatt gccaaataga ttcctagtac
    11221 ttgtcctaaa agaagcaaac ccaaacccac cagcggaagc aagccaagaa aaataaataa
    11281 acctacgcgt ccaatctttt tactttttcc atccgctaga tattgttttg caaaattatg
    11341 atcttttttt cctaaacgag cgaattgata ttggattttt tctctcagtt gaggattttc
    11401 ctgataaaaa gcaatcagtt tttctaactg ctgatcttca ccaaaatcaa agctgcgtaa
    11461 acgttgatac aacgcttctg aacctacact agaataggta ctgttgattc cttcgaatac
    11521 ttcgaacata tccaggtcat accaagtaag atcatcgatt tcgctatccc atgaacggaa
    11581 cttcttctcc gtgagccatg cttccttcaa gctttcttct ttatcaaatc tcggttgata
    11641 aggaattttt tcccatttgc tacgaactgt ttcctttaat ttcaaacgtg aaaaaatgtc
    11701 taaagcaact aataaaataa cagctgcaat aataattaaa acgataaatt ctctttgacc
    11761 catatccctc tccgttcctt tatttgttag ctttagtata gcactgttgc gctatttgaa
    11821 aagagggtaa aaaaacacct ttcaaaataa aaggtgtttt tttcttgtat attacatatt
    11881 tatctctcat ttatcaaaat tgacaaataa tcatagggag gaattgttgt taattcttgt
    11941 ttattctcca gtgcattgac aactgtccca agggaaagga tagcctcagg tactattttt
    12001 tcttgttctg ttggattagc taagcgaaga acaaatcctt gttcatgaaa ataagaagga
    12061 taaatcgatg atacaattaa gtcttcaggt aattcaatca atgaaaattt acgaggaaca
    12121 tcaagttcat tttgtaatgg ccaaatcttg ttatctaacc tgtgaataaa tacattcagt
    12181 ttctgctttt gataagaaat agattgttct aaccgttttt ctgcctgttt tgccactttg
    12241 aactcattaa atcttccttc ttctacatta aatccaaaag tgactttata ctcacctatc
    12301 tcttgagcta gcggtgtttc catcatgaca tgcccttcat ttgtcgtatc tcctgaagct
    12361 cgcccaggtc gccaagcaag attaggtttt ccaagttccc ctgttgttga aaataaggtt
    12421 atgattaatt gattttctat acgttcatac tcttttaaac catctgcata aaacgtaaaa
    12481 taatgatcat tttctgccac agagacactc ttatcgaata tttctaaatt aacaggtttc
    12541 tctaccatag tttctgacca attcggagga atgacatcag gggtattttc aataaatccg
    12601 ttttgaattt gtgcaattga tttttcagat ttatctaaaa cattaattgc taagcgcata
    12661 cgatgcgagt aaacagtatt atgaattttg agtgtgcctt caacgatttg actttctccc
    12721 agactgaacg atatttcata agatatttct tccatttcgg gtttttcaga taaacgatct
    12781 tttaaatctt taggaagttg agcttttccg taaacaacta atgtttcttg taaagaatct
    12841 ttatatactt tagctgtttc taattttaga gatagttctg tatctccttc taatggtgaa
    12901 taatcatacg tatcgccatc atttgctgaa tcgatcaaat gaacgaaatc aagatattgt
    12961 ctatctccaa ctatcaaatt caattgccca tctttaaaag aaagtttata tttattgtta
    13021 cttatctcag ttttgtctat ctcgattgct gtttctaatt tttcattatt tttctcaaat
    13081 tcaacaacag tgtatcctag tgctggcaca tttacattta tttttatgtc aagttcataa
    13141 taagctggtt catcaaaata ttcgaaacct gtagcaatca tccgataagc atttttccgt
    13201 tctggaaaat aaacttcttt ttcaatgata ttatagggac tacctttgaa gtgtattttc
    13261 tttgaatcag ctactacatg cacgattttc gatccactat aattctcagt cgttgtgttg
    13321 aaaacaagga cttcattcga ttttagcttt aatgcatcag ccatccgttt tacaataagg
    13381 ttttcgattc cgtcagctat ctcattagct tctttgattc gatggaaaat atcttccgca
    13441 acattatctg aaacacagcc accaatacta tcatgagcct ggttttgcaa aatctttttc
    13501 cataaccgac gaagtaggcc tttactgata tggataccat ttctttctgc aatcagcatg
    13561 agcggctcaa ctcgttttag aattttttgt tccaatttaa aattttctat tttcattttt
    13621 gttcttactg agccaataga acgatgaaca cgagagtaaa ccggctgacg aagttcacct
    13681 ttatattcag gtaattcttt tttgtttctg ataacttcta cataagaagg ataatctgaa
    13741 ataatattgt ggaatttact gtatttattc aacttctcta cgacttgatc aaaatttaaa
    13801 atcattgact tttgatctac acccgaaggt actaaaatat ctttgttttt tccttgcata
    13861 tgaagcattt caactgcttc atccaacttt tcttttgcaa attcaggaat atctttaatt
    13921 gcgtctaagg tcatcattcc agtcatataa ccaaatggaa aattcatggc ataaacaaat
    13981 ttatctccca atcctttcca gataaaataa ggagatggaa ccatcgtttc aaagtttaat
    14041 cctcgccaac acatgaaatt atcgatttct acttgatgca aaagtgtagg caaattcgca
    14101 ttgaacccaa acgtgtcagg caaataccct accatcattg gatctccata tttaaggatc
    14161 gtatcactga ttccaatcat taagtttctt aaaattgatt cagcatccac taataaggca
    14221 tctgtttgtg cataccacgg tcccacgaat agttgtcctg ttttcatcag tcgttggata
    14281 accttaagct tttgcggatt gatctccaaa tactcatcga cgattgaagt ttgtccatct
    14341 aaacaaaagt ttactttagg gttcctttcc aattcatcta gaacttcagt aaagacctga
    14401 tcagacaata cgatggcatc ttcatttgta aaataccatt ctctgtccca gtgcgtgtgt
    14461 tgaattatat gtgcatttac catatcagat tctcctactt tccttcttct actgaattta
    14521 cttgttgttc ttttttatac ttgatggatt ttagtgcatt gaccattaac gctgtaacca
    14581 ctcccccaat aacaattgat actaaatata aaggtatatt cgtaacaaaa ggaattgcca
    14641 taaaggttga aagcattgtt tcttgagtaa caccaaaaaa tccatttaga ccacatgcta
    14701 ctgcagtgcc caccacgatg cttggtatta ctcgtaaagg atctttcacg gcaaacggga
    14761 tcgtcccttc agtaatcccc acaaggccca taatcatagc tgtctttgca ttatctcgtt
    14821 ccgattcatc ataaagtttt ttgtttaaaa ttgttgctaa aaacattcca aatggcggaa
    14881 tagcaattcc taataacatg gctgtactag gtccaaagct tccgcttgaa aaagaagcaa
    14941 ccccaaaagc atatgcgatt ttattgatag gtcctcccat atcaaacgcc atcattcctc
    15001 ctaaaatagc cgacatcaaa gcagcatttg ctcctgacat gccttctagc caattagtca
    15061 gtcctgttgt cattgcttga ataggcgtag ctagtacata ccataatatg attccaacaa
    15121 ctaatgtagt aacaaaagga atcagcataa ttgattttag actaataact tgcatcggta
    15181 cttttacttt tttcaatgca ttagctaaat agccacaagc taatccagca accattcctc
    15241 caataaatcc cataccaaga ttttgacaca tcatcccagt gataaaagct ggtgctagac
    15301 cgggacggtc agcaattgaa tatgcgatat aggctgaaat catcggtacg ataaactgta
    15361 atcccgtctg accaatcatt cgcaggactc cccagaatgt atccgtctgg tcccaaacac
    15421 ctgtgccacc aaaaatgact gcaatagaag ttagtaaccc tgctgctacg acaagcggga
    15481 tcatatagga aaccccactt tggaaatgat ttagaatttc tcttccttgt ttcttgaatt
    15541 ccattagtaa ctcccccacg atatattttt tatttgttaa ttaatgcaat tgcttcatca
    15601 atcgtctctt caccatcttt tactacctct gaggtcccta ctgttaaaat cggtttaccg
    15661 tcaaagcgac ctcgttctga aacatttgta tctactgcaa atatgacaac atctgcctcg
    15721 gaaacatccg aaattgaaag tttgttttct atccccatag ccccctgtgt ttctacttta
    15781 atatctaacc ctttcttctt cgcaaacttt tctaaattag cttttgccat ataagtatgg
    15841 gcaatccctg cgatacatgc tgttactgct actatttttg tcatatgaaa ttcctccttt
    15901 taaaatgata gagcttttac caattctttt ttatttctcg aatcttttat tcttttaaca
    15961 aactcgtcat ccatcaatct tccagataat tctgctaaaa tacgtaaatg attagtacct
    16021 ttatcagtgt cttttattgc taacaaaaag acgagatcaa ctggttgatc atctaatgcg
    16081 ccccattcaa cagaccgctt taacttggct acagccactg ttgattcaat tactgctagg
    16141 cttttgccat gaggaatggc gatatttttc ccaattcctg tggtcatatg gttttctctg
    16201 tcaaatacgt ctttttgaaa ttgtttgtgg tcagacacaa acccattagt ctctaacaat
    16261 ttcgataatt tttcgactac acttttttta tcagatgcat ttaaatctat cactatatta
    16321 tttatattga ttttttcact gatcacttat aagccttctt tcaatatata aactaatttt
    16381 tcaaacgacg aactcgtttt taaatttgct aataactcat gattcgaagc gatttcattg
    16441 aatgtttcat aaattttatt caattcaggt tttacttttt cattaagtgc aaaaaagaaa
    16501 acagaatgaa caagtgtccc atcccaatca attccttctg gattaacgta gacacagatt
    16561 actggctttt ttataaaatc tacttcgata tgcggcatag caaaatcatt catcgctgtt
    16621 gaagatagtt tttctctttt gattgctccc tctagtatcc cttctttcgc gtaattttct
    16681 tttaccaatt ttttggtaat tatatttaaa acgttcactt gattatcctt tatttggtgg
    16741 gtaaaaaagc aaaatcgttt atctaataaa tttagaaaag catcttctgt ttgttcattt
    16801 aactgtagat actgttttac ttttctgaca tctgccccta acatcaaagg actaacaaca
    16861 ataacaggaa aatttgtatt ttcaatcgga atcgtgctta taacaagttc atcagtgact
    16921 tttctatctt gtagttcatt gatgcttagt acatcttcta tctcaaccat atctgcaaaa
    16981 ccgttattta ttctttgctc taataatttt gatgttccat atccgctact acaaactaat
    17041 atcactttcg ttttgtcagg tttgtaacga tccaaaagtg attgaatatg gagtgttacg
    17101 taagcgattt catcttcatt aaaatgtata cagaatctct cttcgatttc ctctaataaa
    17161 tctacactta tcataaatga ccttttaaaa gaatatttga ttttgtccgt ataagggtta
    17221 tagatattta ctcccaattt gagtctttta actgctgcat tgagatgtaa tactagatta
    17281 ttgatcaatt cttcatcagg ataaaattca tttaatttaa ttcttactaa ctcttgtagc
    17341 tcaacaccca tatctaaata ggaaagcttc gtttgatcta agtcgtgatt catagcattt
    17401 ttttcaatcg aagtaatgtg aatgtcaaca taagcttgtt catctattgg cacaataatt
    17461 gaaaatgctt tttcaatcat tttgattaaa ataagtgtat tctttgagat ttcttcttca
    17521 gaattattac tacgtgataa gaaattgctt gtttcaatcc tttctatcgc aatagcaagg
    17581 tggatagcta aagtttgaaa ttcataatct gtaaatttca attgtgtctt attcatgtat
    17641 cgattaagca attcggctac ttttttcaat atgtccttat ttaagaaatt attcgcttca
    17701 ccaatgaaat tgatattcaa gtctaagaga tctgttttac tgttagatgc tgtaaagcca
    17761 ccccaataat agttcacaac ttctgacatc aaccttcttt tacttttttc atcagctttg
    17821 atgcgcacgc catttcgatc tgaaactaag gacatcccat gcttgtttag tctttttcta
    17881 acatctttca ttgtgctttc taccgtcgac ctgctcacgt ataattcgtc agcaagattt
    17941 tgaatcttta gagaatcatt agtattcaat aatttagcaa acacataagt tagacggtcc
    18001 tccggagagt ctgcgatatt gattttatga ctggataata aatttcgaag tgtttctttt
    18061 tttccaataa gcgaaacgcc ttttttaggt tgaaccaaca tttgtacatc tggcattttt
    18121 tggattattt ctttcaaaat taaaatatct ttgctaattg ttttcgtaga tacatataat
    18181 tgttctgata attctttgta agttgtgtgc tcattttcaa tcaaaaagta tagtattttc
    18241 atttgccgtt cgctaaattc gatgcccatt atcttcacct cctgaataca gcttaagtgt
    18301 acaattatta tctctatttt catacagaag aaagttcttg attaatgtag aagtttctaa
    18361 tattcttgat tatttcaaat caataaaaaa gcctgcaata tttatatcac aggctttttt
    18421 ctctattaaa tatatataag ataatacatt tttctactag ttctcactaa tcttgtataa
    18481 ttaaaccctc cgcaacttga cggagatgga gaagatgccc ccgtctaatc tagcggtcat
    18541 ctctcctccc attccttcca ccaatttttt tgacaggtaa agcccgattc cgctagattc
    18601 cgtatttgtg cggctggtat tttcggtata aaaacgctgg aagacttttt caacagaggt
    18661 ttgcgttcct ggtttgatag gattggccac tgttaatcga atgaatggat cctcttttga
    18721 aaatgtcacc tttgcttgtc catctgcata acgcaataaa ttctgtatga tattttgcag
    18781 cactcgcagc aacatgagcg gatctgctaa tacagttgta tttatcgagt gttcagcgaa
    18841 cgctacctcg atatttttct cagaaaattg ttcataaaaa gagaaaaata cttcttccac
    18901 ctctttagaa aaagaaagcg tatggagatc catttctttt tgcttcgtct ctattcgagc
    18961 aagatcataa aactgatccg tcatactgag taatcttgca acagattgtt tgatgatcgt
    19021 taaatacttc tcccgtttct ccgtatcctc tacctgttcc agtaattgta cataaccgtt
    19081 aatagcagtc aaaggagttc tgagatcatg agaaaggccg acgatcgata gtctgatgtt
    19141 ttcttccatt tcttgaaatg cttgtccttt ttgttcattt tcatccaacc atgtattcag
    19201 tgcatctact aggtccatta gattcttttc tcgaaattcg actgaaagac gaccaccata
    19261 ttcttgtcgt tcagaaagag tggcgatttt cttagttaat ctcttgattt ccctttgcag
    19321 gaagaaaata aaacttccca taaagataac aacgagaagc aacagccatt taatcaaatc
    19381 taaaccccac tccccacacc gtatgaatat acacagcatg tgcctttgct agcttccctc
    19441 gaagattgga aatatgcata ttcactgttt tctcactatc aaaatatggt tcttgccaaa
    19501 ctgtttcata aagatttgct cgcgaaaata cttttttcgg atgttctaaa aaaagcttca
    19561 gcagatcgaa ttctcttcct gtcaaatgga cagccacacc attcacattt acatccctcg
    19621 actctttgtc cagtaagatt tccttgtaat agatgcgttg cgacagatct tttacttgga
    19681 cagaagtctg atgacgaagt tggatgccga ttcgtaacaa aagttcttcg atatcaaatg
    19741 gtttcgtcac atagtcatcc gctcctgctg ccaacaagga aactttatct ctttgatcgc
    19801 tttttgctga aagaacgatg atcggaacat tcgatgtctg tcggatttct gctaacaatg
    19861 cttcacctga aagccctgga agcatgagat ctagaaggat caaatcgaca ggatatgtgc
    19921 ttaacaataa ttttccttcc gtacctgaat aagcatcaag aagtttgtat ctctgttcaa
    19981 gaacctcctc cagtaaggaa tgaacagctt catcgtcttc gataatcaaa atagtttcca
    20041 gcaggaccac ctacttaatg tcagattttt ggaaaataaa cattcctaga gctgatgata
    20101 ccacgattgt tactagactg accagaatca ttttcggtgc ttgcgaataa gttgcttgtt
    20161 gcgcaaagtc cgtatacatg agccacgttt ccgtaaacca ctccgttaga aattccaagc
    20221 ctttgatttt tgcgaaaaca ccgggaatga aggccgttcc catgatataa agaaaagaaa
    20281 gaagcaaact gacactggta cttctagtga tgaatgtcaa agccactgga acagataatg
    20341 tccctaatgt cagcagaagc aatacaggaa atacttttag aacataattt ccatagtttt
    20401 tgattccctc aacttcacca aatccgaaag caagtgtgta agcaagcatg gctagtcccg
    20461 tgatcaaaac cacgccaagt gttgtgatca aaagcgtaac gagaagtttc gacacataat
    20521 aagtcattcg attccgtcct aaagataacg tattgcgaat cgtaccgttt tgaaattctt
    20581 ccccaatata aaagcacgca aatgctgcga aagccagaag aatccatgca gccatttttt
    20641 ctaccattaa aatccctgtt gcaccgttga tgactgtatc cgttgcttgg tttaattcat
    20701 tgccgtattt tgtatgcata taccattcgt tccaagaata aaaaagcgga agtacgacca
    20761 tcagtcctag tgagacccac attttacggc tcttccatac ttttattttt tcagcagtta
    20821 atagattaaa catgtggatt gcctccagtt aattgtaaga aataatcttc cagtttttgc
    20881 ccaactaggt gaataccatc cacaggaact tttgcttcta ccaatgcttg attgatggct
    20941 gctacttggt caattccttc aaaaagctgg ataacacggc tagattgtac aaaataatcc
    21001 ctataaccaa gagagtcgag aaccgttact gcttgttccg tcgccgaagt gtcaattttt
    21061 ataaattgac gagattcttg tgccaattct tctttcgata attctttgat caagtgtcct
    21121 tcatgcaaaa taccatagtg agtggcgatc tgtgacaatt catctagcaa gtggctagaa
    21181 agcaagacgg tgatgccccg ttcttttacc aaacgttgga tgatttctcg catttctgcc
    21241 atccctgctg gatccatccc attcgtcggt tcatccaaaa tcaaaaattc tggattcgtg
    21301 atcagtgcat ttgcaatagc taaacgctgg cgcataccta aagagaaatt ctttgctttc
    21361 tttttccctg tattttccaa tcgcatcatt ttcagcaggt cttcgatttc tctatccgac
    21421 acaccgccgt tcgctgcttg gatacgtagg ttatctcttg cagttaattc tggataaaga
    21481 gcaggggttt caatcgattg acccatacgt cgtcttgctg cctgcaaatc cgtcgttcca
    21541 aataattcga tcgaaccttc atcaatcgag atcaaaccca taatagttct cataaaggta
    21601 gacttacccg ctccattttc cccaatcaat ccatagatca ttccacgttt gatttcgatt
    21661 gatactttat ctaatgcttt ggcagcaccg tattttttgg ttataccagt tgctttcaag
    21721 accgtttcat ttccaaaatc catacttatc cttctttctt attgatgagt gaagtatagt
    21781 tgacgggtat aaagaaagca gccagaaatg tataaagaaa gtgtaaattt tccaattgaa
    21841 tgtcaaaaaa aacaatcgga caatacgtgt ctttcctatt atcaggcagt ttgttatacg
    21901 cttgtatcag tttcctgatt ctttcttttc gaccaaagtc gtttctctgt attcctcttc
    21961 actgaaaatt tttgcttctt tcagatcaag cttgagtaat tttaccagac cggttataag
    22021 gataactata gcaagactcc ctcccagaaa aattagactg tctcgcagac gtggttgcgt
    22081 tgtcttccaa tcaaagagcg caccaaacag tgctaatcca agtggactgg ccaccatggt
    22141 aatcgttgtt atcgttgaca taagtcgtcc ttgtatatgc gttggaactt ctcgctgaag
    22201 ccagaccata taaaaagcaa aagaaaacat taatccttaa aaacggactt atgaaaaata
    22261 atacatgccg tttgggattt acaattagct accaaataag aggttatgac aaaagacttt
    22321 tcacaatccc ttcttccgaa taaacggtgt tctcctgcca actcctcggt cacaagtcac
    22381 aatatttatc aaacatgaga agcaattttt gtaaattcct tcttatttct tgaagctgac
    22441 cacttctgtc acaacctcag aagcggataa ataatattgt ttgatgatac tttatctttt
    22501 tcccgtttgt ttatagatcg aatgatatta tagaccaaga atcctaccta ttaacatagc
    22561 taaaaacaca aaaaaggcac tttccaaatt gtggaaagtg ccagaaaagt taggattaag
    22621 gtaactgcgc tcagcattgc ttcgcccagt acgattctcg taactcgtga actcgaacga
    22681 taatcgcaat taacgttttg agaactgtga agctttacgg gctttcttaa gacctggttt
    22741 tttcttcgta tcattgaata agtttgaaaa accttgattt ataaaggttt tcccgtaaat
    22801 atattttatt atatttgatt aaatttgaaa tgtttgtgtt ctaaaatgtg ttctaattta
    22861 aaggcagtat atttcgtatt ttagattaaa tttttaggct cattcattct caaatccatt
    22921 ctattcagtc aataaaaatt ttaaaaagac tttataaaca aagatgttca agcgagcatc
    22981 ttattttttt gtgcttaatt tgaaaggaga acatttcatg tataaaaaac aaaaaaagaa
    23041 attatgtgta ttaactattt tgagtttggc tgttcaagca attgttggag caattgctcc
    23101 tacgattact tttgctgatg aaattaccca tccacaaacg gtaaccgtag aactagattt
    23161 agcacatcaa tatgctgttg aaggaacttt tagtgatggt cgtccaatgt cagaagtcac
    23221 tgttccgcat tatgcagtgt acaacggctt taaacaagat attttctgta ttgaaccagg
    23281 agtcccgatt tacaatgagt tcactcctgg ctatgagaaa aacccgttac cagatatgtc
    23341 cgataaagcc aaattagttt ccgttttatg gaaaaaagta ggtacagatg cagatactca
    23401 tattgtggca cagaagatga tctggcaaga agttaatggg tatacccttc actcaataaa
    23461 acgatcagat ggtagtgccg taaatatcgc agccattgag gcaaaaataa atcaagccat
    23521 tgacgattat cagaagaaac caaccttcca taatagtacg gctaaaacag tattagggca
    23581 atcgactact gtgacggata cgaataatct gaatttgtca gagttcgatg aggttgtgga
    23641 aaatgccgca aacattgatt atcgtgtgaa tggtaatcag ttgataatca ccccaaatgc
    23701 caattcaaaa gaaagtggtg tgctgacgct taagaaatct gctggtaccg gaacacctgt
    23761 cgcctataaa aaggctggcc aacagacttt aatggccggg gcgattgata aacctaatac
    23821 ctacacaatc aaaattgatg tggaaacgga gggttcttta aagatcaaaa aagtcgataa
    23881 agaatcgggt gatgttgtac caggaacggt tttccattta gactttggaa aaactttacc
    23941 tgcaaaagac gtgactactg acaaagaagg cattgctaca ttggatgaga ttcctcatgg
    24001 tacaaaagta accattaccg aaaaatcagt accggcgcct tatacgattg ataccacccc
    24061 catgactacc accattaaag caggcgaaac catttacgtg acttcaaaaa acgcacggga
    24121 aaaagggcaa atcattctgg ataaaagcgg agttgaaaca gggagtgatc tttggaatga
    24181 caattattcc ttagctggta atacgtttgc cattcgcaaa gacagtccaa ccggtgaaat
    24241 cgttcaagaa atgaccacgg atgaaaacgg tcatgcggaa acaccaaaag agattgctaa
    24301 tgcgttggaa ttgggaacct attatgtgac cgaaactaaa gctagtcatg ggttcgtgaa
    24361 tacgttcaaa cctgtaaaag tcgaattgaa gtatgccaat caaaccgtag cccctgttac
    24421 cagtaacgta aaagggcaaa atcaagaagt cactggcgaa accactttga cgaaagaaga
    24481 caaagatact ggggataaag cacaaggtaa ggcagtcttt gagggtgctg aatacacctt
    24541 gtttactgct aaggatggaa aagctgttaa atggagtgag gcttttaaac cagagttagc
    24601 gaagggaacg aaaggttctg atgaaacggt cactttgact ttagacgaaa agaaccaagt
    24661 tgccgttaaa catttagcta tcaacgagta ttattggcaa gaaacaaaag cgcccgaagg
    24721 atatagcctt gatgaaacaa agtatcctgt atccatcaaa aaggtggatg atagcgaaaa
    24781 aaatgccgtg attacccgaa atgttacggc aaaagaacaa gtgattcgct ttggctttga
    24841 tttctttaaa tttgctggat cagctgcggg cactaccgaa actggcttca atgatctgac
    24901 ctttaaagtg tcgccattgg aagggaccaa tgaaatcaca ggtgccgaag atgaagcgac
    24961 cacagcttat aacgagcaat tagggtttga tggttatggc aagtttgaaa atcttcctta
    25021 tggggattat ttacttgaag aagtagaagc cccagaaggt tttcaaaaga ttacgccatt
    25081 agaaatccgt tctactttta aggaaaacaa agaagacttt gtgaagagtg agtatgtctt
    25141 taccattacc gaacaagacc aaaaacaacc aattaaaacg gtgaccgttc cttacgagaa
    25201 gctgacgaac aaagcatttt ctgttagttt gaaccgttta atgctctatg atttgcctga
    25261 ggaagaagat agtttaactt ctcttgcgac ttggaaagac ggaaacaaag aattaacttc
    25321 tctggattct accgagctag ttgataaatt gagttacaat ctacatgaaa tcaaagaaga
    25381 ctggtatgtg gtagcgcaag ccattgatgt ggatgcgaca aaagctgccc aagaaaaaga
    25441 tgaaaaagcc aaaccagtgg tgattgcaga aacaagcgca acgttggcga acaaagagaa
    25501 aacaggaact tggaaaattc agcataaatt aaccgccgaa caagttttaa ataaaactat
    25561 tgtcttattc aactatgtgt atgaaaataa ggaagctttt gaagcaggcg ataagccagt
    25621 agcgaaggat gttagcttga ataaccaagc ccaaactgtc agttgtacag tggaacacca
    25681 tgtttctatc caaacaaagg cccacctaga aaacggttct cagaccttta ctcatggtga
    25741 tgtggtggat atgtttgatg atgtgtcgat tactcatgat gtactggatg gctcaaaaga
    25801 agctttcgaa acaattctgt atgctttatt accggatggt acaaacaaag aaatttggaa
    25861 atctggcaag attgattatg aagtaaatga taaagaattc accaaaactg tgcttgcgaa
    25921 aaaagtagat accggaaaat atccagaagg aaccaagttt acttttgcgg aaatcaatta
    25981 cgataaagat gggaatatca atggaaaaca caatgaagat ttgaaagaaa aatctcaaac
    26041 cttaacacca aaagaagttc caaccatact aagtacgccg aaacaaccgg aaacaccaac
    26101 tgttccaagt ggctctcaag aatctagtcc cacagtgaag accttccctc aaaccgggga
    26161 gaaaaattcc aatgttttac tgttcattgg ttttactttg atttttgcga cggcgggcta
    26221 ttatttctgg aatcgccgga actaaggtga tgagatgata caagtaaatc ctaagaacca
    26281 acgtgcgcga ccgcctccta aaaaagtagt cgcgctattt ttgtatccaa aactaacaat
    26341 gaaaagagga aaagaagatg gaattgaaat ttgtcgtgcc agatatggct gaaacctttg
    26401 gaaagttaag ttatgccggt gaaggagaaa ttataacaga aggatatgga cggaatacta
    26461 cggtgattgg tcgcagttat catttgtatt ccagtaagca acgggccgat gatattgagg
    26521 tggtagtagc tgcggaagcc ggtgaaaagg attttgatca agaccaaccg ctaaaagccg
    26581 tgaatcccca tttggttgcc aaaggctatg agattgagaa tcgtgggttt actgattacg
    26641 tgttgtatgt tgatgattta gtgaaagcat aggaggaaaa gaagatgaga ttaacagaag
    26701 gtattgtcgt agattcaggt ttaacatttg ggaagttgcg tttttccgca ttacgccgag
    26761 aagtacggaa gcaaaatgag gatggaacaa ttggtaatga ggtaaaagaa cgaacctata
    26821 atttaaaatc ctctgcacaa gggcgaatga ttcaagttag tatcccagct aatgtaccct
    26881 tacgcgagtt tgcttatgat gcagaggtag agttggtcaa tccaatcgtg gataccgttg
    26941 ccaattatgt ttttcgagaa ggaactaccg ttaattggtt cattaaggcg gatgatttag
    27001 tcttgaagcg gcagccgaat caaggaaatc ctacgaacca aaatgaaaga aagaaatagg
    27061 tgaagattat ggaaattaat gttgaacata ttttagattg tctcgatcaa tatggaaaag
    27121 gcgagctgac tgaggaacaa ctcgttaaag cattaacctc tgatgaaaaa atgtttctga
    27181 ttatacatca aggtttactt ggagaaaata gcgacagtga agaagactct gatgtactag
    27241 actggttagg agaagaagag tctttcttta tagttgtcga agtaaatgaa aacttatgtc
    27301 ggcaggcaga atccattttg gaagaaattg gtgtggagat gccggatgct attgaaggct
    27361 ttttaaatca attggttgaa acgaaacaac tcccctcctc gtagcagtaa atgactagca
    27421 taactttttg aaattgccta atgaaatcct attgggcttt ttcgattgga ggtggaaacc
    27481 atcaaaatgt acaaaggaca tcgcatacgc gcaggagatc agcacttggt ttatcacttt
    27541 gttcttggct ggctactcgc tctgttcatc ggttggatga gcgtctttta ttttcaagag
    27601 tttagacaat ttgatatttc taagctatca ctttctacta ttgaaatcgt ttggtctata
    27661 aaagatttag tatgtctgct tggaagtctc gctttttcag gagctatgat attgctttat
    27721 attcactttt ttcttgatca ttggcgaagc ttgtggcatc gacaaaagct agctcggatg
    27781 attctagaga accattggta tgaagtgaaa caaactcaaa gtgaaggctt tttcaaagat
    27841 ttgaatagta gccgaaccag agagactatc agttacttcc ccaaaatctg ttatcgaatg
    27901 aaagatggat tactgtctat tcgcgttcaa atttcacttg ggaaatacca agatcagctt
    27961 ttaaaattgg aaaagaagtt agaaagcgga ctgtattgtg agttagtaga aaaggaactc
    28021 aaagactcct atgtggagta tactttactg tatgatatga tcgccaatcg aattgggata
    28081 gacgaagtag tggcagaaaa tgggactctg cgattgatga aaaatcaagt atgggcttat
    28141 gattctttac cccatatgtt aatcgctggt ggcacgggtg gagggaagac ctatttcctc
    28201 ctcaccatta tcgaagcact attgaagtca gatgcagaat tgtttatcct cgatcccaaa
    28261 aatgcagatt tagctgattt aggtacggtg atgcctcatg tttattcaca aaaggaagaa
    28321 atttctgctt gtgtggaaga tttctatgaa cgtatgatgg ctcgtagcaa ggcaatgaaa
    28381 gaaatgtcta actacaaaac aggagagaat tatgcctatc ttggacttcc acccaacttt
    28441 ttaatctttg atgaatacgt ggcttatatg gagatgttaa cgacgaaaga aagtgcggtg
    28501 attttgaata agctcaaaca aatcgtgatg ttgggtcgtc aatctggttt ctttctgatt
    28561 ttggcttgcc aaaggccgga tgcgaaatat ttaggagacg gaatccgcga tcaattcaac
    28621 tttcgcgtgg ctttgggtcg tatgagtgaa ctcggatatt ccatgatgtt tggggaggtc
    28681 gaaaaaaact tctttatgaa gcacatcaaa ggtcgaggtt atgtggatac aggtggcagt
    28741 gtgattagtg agttttatac gccacttgta ccaaaagggt atgatttctt agattcaatt
    28801 agtaaattag aaaagatgaa tgattgagtg atcttcaata ctgactttat acttcaagta
    28861 acgtataata cttttatata gtaggaagaa aggggataat taatatggaa agtaatgatt
    28921 cttatttaga gtatgcaaaa gcaaataagg ataagtttat ttcttatatt atcaaaggga
    28981 aatcccccga agttggacga gacgcagttt ttatggcagg gagtcctggg gctggaaaat
    29041 cagaggttgc cattggatta gctgagaatt atgataacca tgtgattatt gatgcggatt
    29101 actttcgcac ccagtttcct gattataatg gaaaaaactc tagtctcttt caaaaagcat
    29161 cttcttggtt ggtagagcag gcatttagat atgtcttaga tcatggctat tcttttattc
    29221 tggatgcaac ttttgcaata ttaagtgctg aaaaaaatat ctcgcgggcg ttgaaaaaac
    29281 attttcgagt gacgattttc tatgtttatc aagaccctaa aatcgcttgg aaatttacac
    29341 aagaacgtga aattgcagag ggacgtcatg tgccgaaaaa ggcatttatt aacgcttttt
    29401 tcaaatcaag agagaacatt gcaaaagtaa agagtaaaca tccag
//
LOCUS       DAZBSA010000025        29100 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000025 DAZBSA010000000
VERSION     DAZBSA010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.025"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1031)
                     /locus_tag="ACHGMT_001013"
     CDS             complement(<1..1031)
                     /locus_tag="ACHGMT_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN6834779.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSVVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="ACHGMT_001014"
     CDS             complement(1113..1802)
                     /locus_tag="ACHGMT_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFN6834780.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACHGMT_001015"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACHGMT_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFN6834781.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACHGMT_001016"
     CDS             complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACHGMT_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFN6834782.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3036..3163)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACHGMT_001017"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACHGMT_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFN6834783.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACHGMT_001018"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACHGMT_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFN6834784.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="ACHGMT_001019"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="ACHGMT_001019"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFN6834785.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="ACHGMT_001020"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="ACHGMT_001020"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFN6834786.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7544)
                     /locus_tag="ACHGMT_001021"
                     /pseudo
     CDS             complement(6444..7544)
                     /locus_tag="ACHGMT_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(7636..8523)
                     /locus_tag="ACHGMT_001022"
     CDS             complement(7636..8523)
                     /locus_tag="ACHGMT_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFN6834787.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="ACHGMT_001023"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="ACHGMT_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFN6834788.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="ACHGMT_001024"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="ACHGMT_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFN6834789.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="ACHGMT_001025"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="ACHGMT_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFN6834790.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /gene="cbpA"
                     /locus_tag="ACHGMT_001026"
     CDS             9754..10398
                     /gene="cbpA"
                     /locus_tag="ACHGMT_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFN6834791.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="ACHGMT_001027"
     CDS             complement(10454..10957)
                     /locus_tag="ACHGMT_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFN6834792.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="ACHGMT_001028"
     CDS             11554..12519
                     /locus_tag="ACHGMT_001028"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFN6834793.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="ACHGMT_001029"
     CDS             complement(12618..13790)
                     /locus_tag="ACHGMT_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6834794.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="ACHGMT_001030"
     CDS             complement(13777..14922)
                     /locus_tag="ACHGMT_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6834795.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="ACHGMT_001031"
     CDS             complement(14936..15868)
                     /locus_tag="ACHGMT_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6834796.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="ACHGMT_001032"
     CDS             15985..17064
                     /locus_tag="ACHGMT_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFN6834797.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17679
                     /locus_tag="ACHGMT_001033"
                     /pseudo
     CDS             17061..17679
                     /locus_tag="ACHGMT_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(17789..18016)
                     /locus_tag="ACHGMT_001034"
     CDS             complement(17789..18016)
                     /locus_tag="ACHGMT_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN6834798.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18119..20707)
                     /locus_tag="ACHGMT_001035"
     CDS             complement(18119..20707)
                     /locus_tag="ACHGMT_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFN6834799.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20727..21386)
                     /locus_tag="ACHGMT_001036"
     CDS             complement(20727..21386)
                     /locus_tag="ACHGMT_001036"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFN6834800.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLLNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="ACHGMT_001037"
     CDS             complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="ACHGMT_001037"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFN6834801.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22159..23025)
                     /locus_tag="ACHGMT_001038"
     CDS             complement(22159..23025)
                     /locus_tag="ACHGMT_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFN6834802.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23134..23775
                     /locus_tag="ACHGMT_001039"
     CDS             23134..23775
                     /locus_tag="ACHGMT_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFN6834803.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="ACHGMT_001040"
     CDS             complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="ACHGMT_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFN6834804.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25216..26115)
                     /locus_tag="ACHGMT_001041"
     CDS             complement(25216..26115)
                     /locus_tag="ACHGMT_001041"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFN6834805.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26117..26914)
                     /locus_tag="ACHGMT_001042"
     CDS             complement(26117..26914)
                     /locus_tag="ACHGMT_001042"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFN6834806.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26892..27596)
                     /locus_tag="ACHGMT_001043"
     CDS             complement(26892..27596)
                     /locus_tag="ACHGMT_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFN6834807.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27727..28302
                     /locus_tag="ACHGMT_001044"
     CDS             27727..28302
                     /locus_tag="ACHGMT_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFN6834808.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28420..29097
                     /locus_tag="ACHGMT_001045"
     CDS             28420..29097
                     /locus_tag="ACHGMT_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFN6834809.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8564 a         6167 c         5230 g         9139 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactac acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctag ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcga aacccatcgt cccgatgttt tgctgacgga catccgcatg
    17221 cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc caaagccatc
    17281 cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt ttccatcggt
    17341 gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt caaatccgct
    17401 tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca aatgatgaag
    17461 aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga gcttgcaatc
    17521 atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt gtacttgagt
    17581 gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt acgggatcgc
    17641 acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa cacaaaaaaa
    17701 cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt caattgtccc
    17761 gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca ggattcgttg
    17821 gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt tctagttttg
    17881 attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat tctttttcca
    17941 tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct aatttatctt
    18001 cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca tatcacactg
    18061 aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat ctttaaaatc
    18121 aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca atcatcggat
    18181 ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa acagcaggtt
    18241 ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt tccactgttt
    18301 ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt agcttttctt
    18361 ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt ccgaccaaag
    18421 aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca ttatccatga
    18481 tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa gtgacatagg
    18541 cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc gtgtagagca
    18601 aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc atcttgaatt
    18661 cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta ttccattcgt
    18721 ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc actttatctt
    18781 cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg aatacattca
    18841 attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg gtgtcgttcc
    18901 aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc attttattta
    18961 acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata tcttgtgcac
    19021 ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca atttgataag
    19081 catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt ggcagacgtc
    19141 cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc cgataaatct
    19201 gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt acttgaccag
    19261 gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata ttcgtcggaa
    19321 tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca tcaacaatca
    19381 aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt ccattcagcc
    19441 ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt cgtttagctg
    19501 ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat tttgaaggat
    19561 ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc acagtagttt
    19621 tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct tcagcgatag
    19681 cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc cgaatattct
    19741 tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg cgctggatcg
    19801 aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta gtctcttctt
    19861 gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga tcaggctgga
    19921 tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat gtctccgccg
    19981 gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag atagctgctc
    20041 gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta tctgcacgtt
    20101 tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg atgatatcca
    20161 aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg aacccgtaac
    20221 gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc tcagcaagca
    20281 tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga tcagcaatga
    20341 ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt ttcttcccga
    20401 ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt gttggctttt
    20461 cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc actaattctc
    20521 cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg actacaatct
    20581 ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact ttgtaaaaat
    20641 tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc atttcttctg
    20701 gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt ttcagttcct
    20761 aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca aggctcttca
    20821 gtctctaaaa tcgtcaaact actatttaac aatccaccca tttcacgcaa ctcacttagc
    20881 tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc tacgaataaa
    20941 agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat acgtgtgatt
    21001 gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa atgattacgc
    21061 aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg accttctaaa
    21121 gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg acgagcaagc
    21181 tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga ataaattgct
    21241 ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta actttgtggc
    21301 aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca ttgtgtcttc
    21361 ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt ttattttact
    21421 ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa ttctaaatta
    21481 gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt cgataaattc
    21541 aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc gttttcacgc
    21601 ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg ctcttctcca
    21661 tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa atgtagttta
    21721 cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat atttacatca
    21781 ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa acctagtggc
    21841 tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt tccagtgttt
    21901 gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc tctcatattt
    21961 atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt atcaactcgg
    22021 tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt tatcagaaca
    22081 ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc ctcaactttt
    22141 ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag ttccttttaa
    22201 taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg ctttgattcg
    22261 ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat tttcctctgg
    22321 tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt cagcaaactt
    22381 ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat aaaaagcctg
    22441 acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc catcttctga
    22501 gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa ccgcgttgaa
    22561 tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg cttcggcaga
    22621 ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag cattttgaat
    22681 ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa catctaatag
    22741 cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat acatgccaga
    22801 ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt taaccgcatg
    22861 ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat ttttgcatat
    22921 caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga acagctgatg
    22981 attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc cccctcattt
    23041 ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg atacaatgcc
    23101 tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg cgctgaaaac
    23161 tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt gtgtgacaac
    23221 ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt aagttatgct
    23281 ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga cttcgtctat
    23341 aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa attagggacg
    23401 gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga ggccttagaa
    23461 ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt tgacagtatt
    23521 cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt tgaccgtatc
    23581 ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg gcttaaagaa
    23641 ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt cactgcagag
    23701 aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac caaaatcgtg
    23761 gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa tgcttattgc
    23821 aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata ttgaaataaa
    23881 tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct actgctggat
    23941 caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc gccactgtga
    24001 ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt ttccaaaaac
    24061 ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg gtcaagactt
    24121 cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca agtcgacgaa
    24181 cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc agcgaaatga
    24241 agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt aaagaagcgg
    24301 tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac gctgccttta
    24361 ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa tcgctagcag
    24421 cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac aaatgatcat
    24481 cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt gccacatctt
    24541 cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca gaaatcattg
    24601 cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc tgaccttctt
    24661 gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc ttcagcacgt
    24721 acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt gtcatgatcg
    24781 cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca tcgtctgccg
    24841 acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc aagtcaacag
    24901 cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc atttcatcta
    24961 ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc gctaattctt
    25021 taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat tggtagaatt
    25081 gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga atattatttt
    25141 cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt tcatccaaac
    25201 caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt ctgcggctaa
    25261 aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca gatcataaca
    25321 atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat catcaccgag
    25381 cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc ctgtatgcag
    25441 ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact cagtctgtgc
    25501 cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc gggcgatcgg
    25561 caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta aggcctgata
    25621 ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg caaatccatg
    25681 ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca catggattac
    25741 ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca ttgtcccatt
    25801 tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta ccagaacgtg
    25861 ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt catgttctcc
    25921 ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca agacattttc
    25981 tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta acagaccctt
    26041 tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt tctcataatc
    26101 ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga tgccaaaaat
    26161 gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa taaattgagc
    26221 ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct tctgtatttt
    26281 ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc aaagtacgaa
    26341 acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata ctaggatgga
    26401 tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta gaaatcgtca
    26461 atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg gctaccatcg
    26521 tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg tccggtgttt
    26581 catccaaata actgatccga acatccagca agggataact aacactttgt tcccggttcg
    26641 ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg taaaacccta
    26701 aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg tagtgatgga
    26761 acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc tcatgatcat
    26821 ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact tctatagtct
    26881 ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct ttcgctttct
    26941 ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg ggagaataac
    27001 cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa ctgataggca
    27061 gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc ttgatattta
    27121 taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg aatctggatt
    27181 tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat caccagatga
    27241 taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg gagaactttc
    27301 atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac aaactgacac
    27361 atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat tggaatatgc
    27421 cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt gacaaattca
    27481 atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag tttcactttc
    27541 aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg ttccatagct
    27601 gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa gttttttcgt
    27661 actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca aaaaggagtc
    27721 gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa gcaagagtac
    27781 gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca gactaatatt
    27841 tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg gatccgtttc
    27901 gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg tctcgaaaca
    27961 acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg tctgcctcgt
    28021 tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc attaatccaa
    28081 accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact tttagctatt
    28141 gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt aggggatgca
    28201 tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc ttatcgtcct
    28261 gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt cattttcaca
    28321 aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg aaagaatcta
    28381 gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat ctatttattt
    28441 gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca atttatcgaa
    28501 gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc tttggtcaat
    28561 ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa tcttgaggaa
    28621 cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc ggcgcagttt
    28681 caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt cggctgtaat
    28741 gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg cggcgatttg
    28801 aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt ttctgatcaa
    28861 aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta caattatgcc
    28921 tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct ttggagaatc
    28981 acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga acatttacat
    29041 acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc taattaaaaa
//
LOCUS       DAZBSA010000026        29054 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000026 DAZBSA010000000
VERSION     DAZBSA010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.026"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(140..913)
                     /locus_tag="ACHGMT_001046"
     CDS             complement(140..913)
                     /locus_tag="ACHGMT_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834810.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            1018..1263
                     /locus_tag="ACHGMT_001047"
     CDS             1018..1263
                     /locus_tag="ACHGMT_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN6834811.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            1419..2270
                     /locus_tag="ACHGMT_001048"
     CDS             1419..2270
                     /locus_tag="ACHGMT_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HFN6834812.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            2303..2980
                     /locus_tag="ACHGMT_001049"
     CDS             2303..2980
                     /locus_tag="ACHGMT_001049"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFN6834813.1"
                     /translation="MDKKVLIKRFSEAIEKGNAAIFAGAGLSMSQGYVSWPELLNDPA
                     TEIGLDSKKETDLVTLAQYYKNENGGSRGILNQILMDNFGEELEISENHRILASLPIE
                     TYWTTNYDHLIEKSIREAYKNPQVKKNYTQLATTNPNVDTIVYKMHGDIDDVSSTVIT
                     RDDYEKYDDSYALFKETLKGDLLTKTFLFLGFSFTDPNLERILSDIRWVLRENQRPHY
                     CIMRKIL"
     gene            3468..4433
                     /locus_tag="ACHGMT_001050"
     CDS             3468..4433
                     /locus_tag="ACHGMT_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6017 domain-containing protein"
                     /protein_id="HFN6834814.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            4455..4667
                     /locus_tag="ACHGMT_001051"
     CDS             4455..4667
                     /locus_tag="ACHGMT_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFN6834815.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            4742..5218
                     /locus_tag="ACHGMT_001052"
     CDS             4742..5218
                     /locus_tag="ACHGMT_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HFN6834816.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            5215..7023
                     /locus_tag="ACHGMT_001053"
     CDS             5215..7023
                     /locus_tag="ACHGMT_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFN6834817.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            7100..7315
                     /locus_tag="ACHGMT_001054"
     CDS             7100..7315
                     /locus_tag="ACHGMT_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family mobile element protein"
                     /protein_id="HFN6834818.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            7407..8270
                     /locus_tag="ACHGMT_001055"
     CDS             7407..8270
                     /locus_tag="ACHGMT_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB6/TrbL-like conjugal transfer protein,
                     CD1112 family"
                     /protein_id="HFN6834819.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            8307..8843
                     /locus_tag="ACHGMT_001056"
     CDS             8307..8843
                     /locus_tag="ACHGMT_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFN6834820.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            8857..9279
                     /locus_tag="ACHGMT_001057"
     CDS             8857..9279
                     /locus_tag="ACHGMT_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HFN6834821.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            9209..11608
                     /locus_tag="ACHGMT_001058"
     CDS             9209..11608
                     /locus_tag="ACHGMT_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HFN6834822.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            11640..13631
                     /locus_tag="ACHGMT_001059"
     CDS             11640..13631
                     /locus_tag="ACHGMT_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFN6834823.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            13654..13905
                     /locus_tag="ACHGMT_001060"
     CDS             13654..13905
                     /locus_tag="ACHGMT_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HFN6834824.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            13895..15124
                     /locus_tag="ACHGMT_001061"
     CDS             13895..15124
                     /locus_tag="ACHGMT_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1107 family mobile element protein"
                     /protein_id="HFN6834825.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            15121..17202
                     /locus_tag="ACHGMT_001062"
     CDS             15121..17202
                     /locus_tag="ACHGMT_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFN6834826.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            17351..21271
                     /locus_tag="ACHGMT_001063"
     CDS             17351..21271
                     /locus_tag="ACHGMT_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HFN6834827.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKTD
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLIERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            21272..22216
                     /locus_tag="ACHGMT_001064"
     CDS             21272..22216
                     /locus_tag="ACHGMT_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834828.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            22220..22423
                     /locus_tag="ACHGMT_001065"
     CDS             22220..22423
                     /locus_tag="ACHGMT_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HFN6834829.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(22723..22959)
                     /locus_tag="ACHGMT_001066"
     CDS             complement(22723..22959)
                     /locus_tag="ACHGMT_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6834830.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(23142..23513)
                     /locus_tag="ACHGMT_001067"
     CDS             complement(23142..23513)
                     /locus_tag="ACHGMT_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFN6834831.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(23510..24838)
                     /locus_tag="ACHGMT_001068"
     CDS             complement(23510..24838)
                     /locus_tag="ACHGMT_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFN6834832.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(24799..25128)
                     /gene="mobC"
                     /locus_tag="ACHGMT_001069"
     CDS             complement(24799..25128)
                     /gene="mobC"
                     /locus_tag="ACHGMT_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFN6834833.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(25386..25756)
                     /locus_tag="ACHGMT_001070"
                     /pseudo
     CDS             complement(25386..25756)
                     /locus_tag="ACHGMT_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            25901..26086
                     /locus_tag="ACHGMT_001071"
     CDS             25901..26086
                     /locus_tag="ACHGMT_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFN6834834.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            26244..26411
                     /locus_tag="ACHGMT_001072"
     CDS             26244..26411
                     /locus_tag="ACHGMT_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834835.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            27029..27688
                     /gene="vanR-B"
                     /locus_tag="ACHGMT_001073"
     CDS             27029..27688
                     /gene="vanR-B"
                     /locus_tag="ACHGMT_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFN6834836.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            27691..29034
                     /gene="vanS-B"
                     /locus_tag="ACHGMT_001074"
     CDS             27691..29034
                     /gene="vanS-B"
                     /locus_tag="ACHGMT_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFN6834837.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
BASE COUNT         7513 a         7367 c         7651 g         6523 t
ORIGIN      
        1 gaaatagata cagattatta tcaaccacaa gaagttttcc aatcatttag aaatcagatt
       61 attcgctcag gtaagaccgt aaactgaaaa acaataaaag aaacgttgaa agaataatta
      121 ttccttcaac gtttcttttt tagaatgttt taatgaccaa agcagtttca ttgtcaggct
      181 taggtaattc atgaaatccc gtatttatag tgctcaattg atcaactaac catgttttac
      241 tttttgaatt gtagataaat ctaaaattaa tatcgttatt atcgtgttga aggggcgtct
      301 ccaaataatc tgctgtatat tcatagtcaa cgtaacctgc taatacaaaa gagagtatcc
      361 ctttatttaa tgttagattt aatggataat catacgactt tatttttttc aacttcatat
      421 tgtacatatc ttcagccata aaagttgagt tcgtgatttc atcttgggtc tcattaaaaa
      481 agttatccga ataatcgctt atttttgaaa agtctttatt ggcaccagcc tcaaaaaatt
      541 gttcaacttt ttcagtataa agttgtgcta acgctccttt ttcaatatta actttaatat
      601 tttcatgtct atcaatttgt gtgctgttca gtaagggaag atcagctttt agttttatat
      661 cgtcttgatt tctttcaatt tcaattggca aaatcttttc agtagattca gtttctgata
      721 aaggaacatc tagtttttta tcatgataat aagcttcaaa tccatctgga accggaaaaa
      781 aatgtattgg gtaattcttt tctgaggttg ttgtactagt ttccgtactt gtagaacttg
      841 tgtcagaagt ggtacacccg cttatagttc cgatgagtaa agcgcaagtt agaatgattg
      901 caaatttctt caaaaaaatt cctccttaaa attgaatatg gactaatact agtcccattg
      961 tagcataccg aatctttaag attaataaga aaaaggacta attttagtcc aaaaggtgtg
     1021 ctattcttga aagaggaaaa aattattgat aaagaaaatg taatcggtag caatattaga
     1081 cgagtccgat tagaaaaagg aattggtcaa accgagctga ttagagacct acagcttcgt
     1141 aaaattgcta taactaggga aacgttggtt aagattgaag gtggccgcca acacattaag
     1201 ttggatcaat tgaaagccat caaagatatt ctacaggtgt cttatgaaga tttgcttcaa
     1261 tgactacttg tagtcatttc gtttgaaatt attatgttac acccttatgt gtcacgaaga
     1321 aatactggta taataatgaa tgggatatgt aacgataggg tatactgtta tgttgcctaa
     1381 taaattgtgg atcaaaatac aagaatggag ggatatcctt gtataaggca tatgaattgg
     1441 attttacaaa tgctgactta aatgcattct cctcaagctc gcaatcatat acacatgtag
     1501 taaatcaaat agatcacaac caaaaatcta ttaacaagag aatagaaaat ctatttgaaa
     1561 gtgaaaatga tttagttgat tattattcta aggacagcaa aattctagat gcagacgaaa
     1621 ttattaaaga ttggtttcca actataaaag cagatatttt tatatctcac tctcatgctg
     1681 atgaaaaatt agctatacgt tttgcatcat ggctatttga aaattttggt cttactgcat
     1741 ttattgattc ttcggtttgg ggatacagtt ctgatttatt aaaaaaaata gaccaaaaat
     1801 attgctataa ggaacaaaca aaaacatatg attatgataa aagaaatgta acaactagtc
     1861 atgttcatat gatgctttct acagctttaa ataatatgat tgatagtaca gaatgtctgt
     1921 tttttcttaa tacacctaat tcaattagtt tgagcaatga aatcactaat gaacagaaat
     1981 ttacgtattc gccatggcta tatagcgaac tgacgacagc ttcaatagtg gaaaaaaaga
     2041 atccacgatt ggaaagtaat cctcaaatga gcaccgagga tgttagaagc attataaagc
     2101 actattcttc gcaagaaaat ctgcaaataa aatattcttt aaatttaagt gaattaactt
     2161 atttacgaaa agctgatttg aaaaaatggg ctttagatgc tgtggaatgc actaaaggaa
     2221 gtaatacatt agatatttta tataaaaaca ccattttcac taatctctaa tcaatttact
     2281 gaatcgaaag gaaccgggta gaatggataa gaaagtttta ataaagaggt tctctgaagc
     2341 tattgaaaaa ggaaatgcag caatatttgc aggtgcagga ttatcgatgt cacaaggata
     2401 tgtaagttgg ccggaactac ttaatgatcc tgcaacagaa atcgggttgg attctaaaaa
     2461 agaaacagac ttagttactc tggctcaata ttataaaaat gaaaatggtg gatcaagagg
     2521 aattttgaat cagatactta tggataattt tggtgaagaa ttagaaatat ctgaaaatca
     2581 tcgaatacta gcaagtttac ctattgaaac ttattggaca actaattatg atcatctaat
     2641 agaaaaatca atccgagaag cttataaaaa ccctcaggtt aagaaaaatt atactcagtt
     2701 agcaacaact aatccaaatg tggatacaat tgtttataaa atgcatggtg atatagatga
     2761 tgtcagttcg acagtaataa caagagatga ttatgaaaaa tatgatgatt cgtacgcgtt
     2821 atttaaagaa actttaaaag gagatttgtt aacaaaaact tttctctttt taggatttag
     2881 ttttacagac cctaatttag aaagaatttt atcagatata agatgggttt tgagagaaaa
     2941 ccaaagacct cattattgta taatgagaaa aatattatag aaaaaatttt aggttatcgc
     3001 tgaaagcccc ggaaatacgg gcatttcgca ggatatgaga actcaattta ctaccaattt
     3061 actactttcg gattgagccg catatatgct gaaccatact gctaataccc gccaccccaa
     3121 atacggcaca tcataaattg aatacgacac cgtaaacgcg gcgttcctct ttcccgtcat
     3181 gggagaacag gacgccgctt ttttatgccc aaatagagaa aggaggcgac gccatgctat
     3241 ttacttccgg ccctgaccgg gcgtttgaac ggctgatgca ggaaaagccc ggtttcgatc
     3301 actacgacgg cggcggggcg gacgcgccgg aggattgcgg cacctgccga ttttaccgcc
     3361 cggattggaa atatcaattc tgccagtttg cagagtgtcc ctatcagccg ggcaagctga
     3421 ccgactatgc agcggtcaaa ttcacagtga aaggagcgaa caacgctatg gcagttttcc
     3481 gtgtggaaaa gaacaaaggc tatacggtga tgtcaaatca ccacctgcgg aataagaacc
     3541 tgaccttaaa ggccaagggc ctgctgtcgc agatgttgtc cttgccggag gattgggact
     3601 ataccctaaa aggactgtcc tatatcaacc gggaaagcat cgacgcgatc aggacagcgg
     3661 tatgggagct tgaaaaagct ggatatatcc ggcgtgagca gggccgtgac gcaaaaggca
     3721 aaatggccga tatggtctat accatctacg aacagccggt attggaaaac ccggtattgg
     3781 aaaatccaac atcggataac ccggtgttgg aaaatccgac atcggataat ccggcgtcgg
     3841 aaaatccaac gcaattaaat aaagaagtac aaagaactga cttatcaaaa aaagaaaaaa
     3901 taattacaga tggacaaagt acccattcca ttcctatccc atccccgacc cctgccccta
     3961 tggagggcga ggcagcggct acgccgccgg aacggaaaag gacaggaaag gatgaggctg
     4021 tacggattta tcgggaaatc attttggaga acatcgagta tgcctatctc attcaggaca
     4081 ggagcattga ccgtgaacag cttgacgaaa tcgtagacct gatgctggaa accgtctgca
     4141 ccgcccgaaa gacaatccgt gtcgccgggg acgactaccc tgccgagctg gtgaaatcca
     4201 agtacatgaa gctgaccggc gaacatatcc gctttgtcct tgactgtctg cgggagaaca
     4261 caacgaaagt ccgcaatatc aaacaatacc tgcgggctat gctgttcaac gcccccagca
     4321 ccatttccaa ctactacacg gcccttgtcg cgcacgacat ggcacagccc gattggggaa
     4381 agcctaaatc cggcctgccg gactattcct gcggcgaggg cgaaagcctg taacgagaaa
     4441 aggaggacga ccctatgaag cagggcgcat tgatttttga cgagcgaaca gaccgttacg
     4501 acatccgctt tgatctggcc gactattacg gcggcctgca ttgcggggag tgtttcgacg
     4561 tgatgatcgg cggcaaatgg aggcccaccc gcattgaaat ggccgaaaac tggtatcttg
     4621 tcggcatccg ggccgacgac ctttccggcc tgcgggtgcg gatttgacct atatctacct
     4681 gcggcgtctg gtggttccgc cttagacgcc gcttttgact ttctgacaag gagggatgcg
     4741 attgcaggaa gaagttactc aaaagaccat tgccctgtcc atgaaaacgg gcaagctcac
     4801 ggctcaagtg ctgcaagcgg cgctgaaaaa gtacctgcaa caccgggcca agggcaaaaa
     4861 cacgctgcac cacggccagc agagcctaaa gcagcttaaa aagcatgggg ctgatctctc
     4921 caacattgag atcaccgagg ccaacattgg agcgttcaag ccatgcgcca agaaatacgg
     4981 cgtggatttt accttacgaa aagataagac cacccagccg ccccactacg ttgtgatttt
     5041 caaggccaag gatgcagata accttgatct ggcgtttcgg gagtttaccg ccaagacgct
     5101 ttccaaggag cagcggccct ctatccgcaa ggttttgtcc gctgtgaagc aaaaggcggc
     5161 ggcccagacc aagcagcggg ccaaggagaa aatcaaggaa agggggctgg aactgtgaag
     5221 cctgacgtaa agaaattgct gatcctgaac ctcccgtatc tgatttttgt gtacctgttt
     5281 gcgaaatgcg gggaggcgta ccggctggcc gctggtgctg acctgtcggg gaagctcctg
     5341 cattttgcgg atggttttgc ggcggctttt gccaaccctc tgccaagcct gcacctgttt
     5401 gacctgtgta tcggtatcgc cggtgccgtg atcgtgcggc tggtggtgta ctgcaagagc
     5461 aagaacgtga agaaataccg caaaggcatg gagtatggca gcgcccgctg ggggacggcc
     5521 aaagacatag cgccttacat cgaccccaag tttgaaaata atatcctgct cacccaaacg
     5581 gaacggctta ccatgaccgg caggcccaaa gaccccaaga cagcgcggaa taaaaacgtg
     5641 ctggtgatcg gtggcagcgg ttcagggaaa acgagatttt tcgtgaaacc caacctgatg
     5701 caatgctttc cgaccacgga ctatccgact tcgtttgtgg tgacagatcc gaaaggcact
     5761 ttggttctgg aaaccggcag gatgttccag caggcgggct accgcattaa aatcctgaat
     5821 accattaact tttccaagtc catgaagtac aacccctttg tctacatcca ttcggaaaag
     5881 gacgtgctaa agctggtaaa taccctgatt tccaacacca agggcgaggg ggaaaagtca
     5941 gcggaggatt tttgggtgaa gtcggaacgg ttgttttatt cggccctgat cggctatatc
     6001 tggtacgagg ccccagcgga ggaaatgaac ttctcaacgc tgctggaaat gataaacgcc
     6061 agtgaagccc gcgaggacga cccggagttt caaagccccg tcgatctcat gtttgagcgt
     6121 ttggagcaga aagaccccga ccattttgcg gtgcgccagt acaagaaatt cctgctgtcc
     6181 gcaggcaaga cccgttcttc catcctgatt tcatgcggtg ccagacttgc gccctttgac
     6241 atccgggagg tacgcgagct gatggaggac gacgaaatgg agctggacac catcggggac
     6301 gaaaagacgg tgctgtatct cattatgagc gatacggaca cgacctttaa tttcattctg
     6361 gctatgctcc aaagccagct aatcaacctg ctttgtgacc gggctgatga tgtgtacggc
     6421 gggcggctgc ctgtccatgt acgcctgatt ttggatgaat ttgccaacat tggccaaata
     6481 cccaatttcg ataaactcat tgccaccatt cgaagccgtg aaatatcggc ctctattatt
     6541 ttgcagagcc agtcgcagct aaaagcgatt tacaaggacg ccgccgagat catttcggat
     6601 aactgcgata gtgtgctttt ccttagtggg cgcggcaaaa atgccaagga gatttccgat
     6661 gcgctgggga aagaaacgat tgacagtttc aacaccagcg agaaccgggg aagccaaacg
     6721 tcccacggat tgaactatca aaaattagga aaggagttga tgtcccaaga cgaaattgcc
     6781 gtgatggacg gcggcaagtg tatcttgcag ctacggggtg tgcgcccgtt tttatcggag
     6841 aaattcgata ttaccaagca tccccggtac aaataccttg ccgacgccga caagaagaat
     6901 acctttgacg tggacagatt tttgactatc acgcgccgga aacggcagca agtggtaacg
     6961 caggatgaag tattcgacct gtatgaaatc gacctgtcgg acgaggacgc cgccgctgaa
     7021 taaacagcgg cttttttcat tccaacaaca aaatcaaaaa atcattatga gccggaaacc
     7081 ggcagaaaga ggtaaattta tggcattttt taattccgca gttactactt tgcagactat
     7141 cgtgatcgga ctgggcggcg ctctgtgcgt atggggcggc atcaatctgt tggagggata
     7201 cgggcaggac aatccggggg caaaatcgca gggtgtcaag cagcttgtcg ctggcggcgg
     7261 cgtggccctg atcggtgtca ctctggttcc cctgctgtcc gggctgctgg gctgatcctg
     7321 gcctttgtcc ctgtccaaac ccttaactaa tcgcctccgc gtcctcccgg ccagaccggg
     7381 agggcgcggg aaaggaggtg ctacccgtga ttagtgaaat cattgaagaa tggataaaag
     7441 gcatcctcat ggacggtatc acgggcaacc tgtccgggct gttcgatacg gtaaatgcca
     7501 aagtcggaga aatcgccgcc gacgtgggac gaaccccgca ggcatggaac agcggtattt
     7561 tcaatatgct gcacagcctg tcggaaaccg tgatcgtccc catagccggg gccatactcg
     7621 cccttgtcat gtgctatgag cttatccaga tgataacgga aaagaacaat atgcacgact
     7681 ttgacagctc catgttcttc cgctggatat tcaaatccgc gtttgcaatc ctgatcgtaa
     7741 cgaacacatg gaacatcgtc atgggcgtat tcgacgccac ccaaagcgtc gtcaatcaaa
     7801 gcgccggtgt gattatcggg cagacatcca ttgattttaa caccctgata ccagaccttg
     7861 aaagccaatt ggaggctatg agcgtgggcg ctcttttggg gctgtggttc caaacccttg
     7921 ttgtgggcct caccatgaat atcctttcca tctgcatttt ccttgtgacc tatggccgca
     7981 tggtggaaat ctatgttgtc acgtcgctgg ggcctatccc ccttgccaca atgggcaacg
     8041 cggaatggcg cggcatgggc caagggtatc tgaaatccct gctggcgctg ggcttccaag
     8101 cctttttaat catggttgtg gtgggtatct acgcggtgct gatacagaat atcggttccg
     8161 cttccgacat atccggcgca atctggggct gtatgggcta tacggtgctg ctgtgcttct
     8221 gcctgttcaa gaccggcagt atatcaaagg ccgtattcgg ggcgcattga tcccaaaatc
     8281 caagcataca caggaggaac ttgtttattt caacgtacaa catcatttac gctgacccgc
     8341 cgtggcggta tgactgcaaa aacgtacagg gcgcagcgga aaatcactat tccaccatga
     8401 gcattgacga gctgtgcgcc ctgccggtgg aacggctggc gtccaaagac tgcctgctgt
     8461 ttttgtgggc cacattcccc atgctgccgg aagctctgcg gttaatcaaa gcgtggggct
     8521 tttcattcaa gaccgtggct tttgtatggc tgaaacagaa ccgtaaaagc cttacatggt
     8581 tttacgggct gggccgctgg acaaggggaa acgcggaaat ctgcctgctg gcaacacacg
     8641 gcaagccgaa gcgtcgttct gcctctgtcc atcagtttat tatcagcccg attgaacaac
     8701 acagcaaaaa gccggatgtg acccgtgaaa aaatcgtaga gctggcgggc gatttgcccc
     8761 gcgtggagct gtttgccaga cagaaaaccc ccggctggga cgtgtggggg aacgaggtgg
     8821 aatgtgacct tatcctgacg tgaaaggagg tgccaaatgg cttatgtaac cgtccccaag
     8881 gacttaaccc atgtgaaaag caaggttgtg tttgggctta ccaaacggca actgatctgt
     8941 ttttccgggg cgctggtgat cggcgtcccc ttatattttc tcacccgtga ctatcttacc
     9001 aacagcgcgg cggccctgct catggtattt gccatgctgc cgggcttttt gcttgccatg
     9061 tacgagcgtc acggccagcc ccttgaagtg gtgattatgc agatcatcca gtgctgcttc
     9121 atccagccaa aggagcgtcc ctatcagacc gacaacacct acaccgccct tgtgcggcaa
     9181 taccagatgg aacaggaggt aaaggccatt gtccaaaaag gaaatcccaa gagaaaccga
     9241 aaaaacaaag ctgacccgcg cccagcgaaa agaaattgac gcggtaatcc gtaaatataa
     9301 gggcgacggc aggccccaca cggcgcagca gagcatcccc tacgaggtca tgtacccgga
     9361 cggcgtgtgc cgggtatcgc ccggcgtctt ttccaagtgc attgagtttg cggacatcag
     9421 ctatcagctt gcccagccgg acacgcagac ggctatcttt gaaaaactgt gcgacctgta
     9481 taactatgtg gacgcctcca ttcatatcca gttttctttc ctcaatcgga aagttgaccc
     9541 ggtgcagtac gccaagagct ttgagatcgc gccgcagggg gacgacttcg acgacatccg
     9601 ggccgagtac accggcattc tgcaaaagca gcttgcaaac ggcaacaacg gcatggtaaa
     9661 gaccaaatac ctgacattca ccatcgaggc cgaaagcgtg aaagcggcgc gagccagatt
     9721 gaagcgcatc ggctttgacc tgttaggcta tttcaagagc atgggggccg tagcccatgt
     9781 gatggacggc tgggaacggc tgaacctgct gcatggcgtg taccacccgg acggcgagat
     9841 attcaatttt gactggaagt ggctggcccc gtccggcctc tccaccaagg attttattgc
     9901 cccgtcctcc ctgtgcttcg gcaatgcaaa gacattcggc atgggcggca aatacggcgc
     9961 ggtaagtttc ctgcaaatcc tgtccccgga gctgtcggac gatatgctgg ccgacttcct
    10021 caatacggaa agcggtgttt tagtcaatct ccatgtgcag gccattgaac agaccaaggc
    10081 gatcaagacc atcaagcgca aaattaccga ccttgacgct atgaagattg cggagcagaa
    10141 aaaggccgtc cgcagcggtt acgatatgga cattcttccc agcgaccttg ccacctacgg
    10201 cgaggacgcc aagaagctgc tgacaaagtt gcagacccgc aatgaacggc tgttccagtt
    10261 gacgttcctt gtcctcaacg tggccgacac caagcaaaag ctgaataacg acgtgttcca
    10321 agccgcaggc gtggcccaaa agcataactg tccccttgtc cggctggact accagcagga
    10381 acagggcctt gcaagcagcc tgccgctggg cgtcaatcag atcaagatac agcgcagcct
    10441 tacgacttcc agcgtggccg tgttcgtccc ctttgtgacg caggagcttt ttcagggcgg
    10501 cgcggccatg tattacggca tcaacgccaa gtcccgcaac atgattatgc tggacaggaa
    10561 gcaggcccgt tgccccaatg ccttgaagct cggcaccccc ggcagcggca aatccatgag
    10621 ctgtaaatcc gagatcgtca gtgtgttctt aacgacgcct gacgatattt ttatttcaga
    10681 cccggaggcc gaatattacc cccttgtcaa acggctccac ggacaggtaa tccggctgtc
    10741 gcctaccagc aaggactttg tgaacccgct ggacatcaac ctgaattatt cggaggacga
    10801 taacccgctg gccctgaaat ccgactttgt gctgtcgttc tgtgagcttg tcatgggcgg
    10861 caagaacggg ctggaagcca ttgaaaagac cgtgatagac cgggctgtgc gcgtgatcta
    10921 ccggccctat ctggcagacc cccgcccgga gaatatgccg atcctgtccg acctccacaa
    10981 agcgttgtta gatcagcatg tcccggaggc tgaccgggtg gcgcaggccc ttgacctgta
    11041 tgtgtccggc tccctgaacg tatttaacca ccgtaccaac gtggacatcg gcaaccggct
    11101 tgtgtccttt gacattaagg agctgggcaa gcagctcaaa aagctgggga tgctcattgt
    11161 ccaagaccag atatgggggc gcgtcacggc caaccgaagc caaggaaagg ccacatggta
    11221 ttttgccgac gagttccatt tgcttttgaa agaggaacag acggcggcct attctgcgga
    11281 gatttggaag cggtttagga aatggggcgg cattccaaca ggtgccaccc agaacgtcaa
    11341 agacctgctt tcctccccgg aaattgaaaa tatccttgaa aacagcgact tcattacgct
    11401 gctcaatcag gcgtccgggg atagaaaaat ccttgcggaa cgcttgaatt tgtccaccga
    11461 acagcagaag tacatcgaca attccgaacc cggcgagggc cttttgatct ttgaaaacgt
    11521 ggtgctgccc tttaccaacc ccatcccgca caacacccag ctttacaaga ttatgacgac
    11581 ccgcttaaac gaggtggcgg gagtatgacc cggcgacgcc acaaaggagg tgaaccacca
    11641 tgaaagagct gaaagccaag gcgaaagtga cgcaggccat gacccgcgac gggctgacgc
    11701 tggaaaatca ggccaccggc gaggtggaga acatttccag ccgggaggcc gaacaggatt
    11761 tgtccgcgcc ggggccgggc ggcaccgccg ataagctgct ggaacgggcc gacgccatcc
    11821 atgacggcca taaggccaag aaagccgcga agcaggccgg ggctgtggcc gccgaggggg
    11881 acgctgccct gcaccggcca tccgcccggc tccaactgtc ggaggaagaa cgggccgacc
    11941 cggatttaca gccctatatc cgaaaatcgg acaaacgggc cgataagctg gataaggccc
    12001 gcgccgccat ccccaagaag cgcgtcccgg tcaaggaaac cgtctttgac gaggcaagcg
    12061 gcaaagcaaa gaccgtcctg cactttgaca aacaggacaa ggggcctccc tccctgaaac
    12121 ccaatccggc aggccgtcct gtttccgagg tttttctatt cggccacggg aaaatccatg
    12181 aagtcgagca tgaaaacgtg ggtgtggagg gcggccataa gggggaggaa cttgccgagc
    12241 ggcaggccgg aaaagccatc cgcagcacca tccggcacca caggctgaaa ccctactggg
    12301 acgcggaaaa ggccgagcgc aggctattgt ctgccaacgc ggagtatttc taccagaaat
    12361 ctttgcggga taacccggag atcgcacagg cggccagcaa ccccatttcc cggttctggc
    12421 agaaacagca gatcaagcgc cagtacgcca aggccgcccg gacagcgggc cagaccgcag
    12481 gcgcggcggc acagggcgcg gccaagacag ccggggcctc cacgaaagcg gccaaggccg
    12541 cagccaaaga aagtgaacgg gcggcctcct ttgtggcccg ccactggaaa ggggcgctgg
    12601 ccgtccttgt gttggggctt ttgatgctgc ttatcatcgg cggcttacag tcctgcaccg
    12661 ccatgttcgg cagcgccgga agcggcatca cggcgtcctc ttacttttcc gaggatagcg
    12721 atatgttggc cgccgaggac gcctacgcgg agctggaagc cgagctgcaa gactatctgg
    12781 acaactacga ggccacccat gactatgacg aataccattt tgaccttgac gagatcagcc
    12841 atgaccccta tgtgctgatt tccattctgt cggcgctcca tgacgggata tttaccatcg
    12901 gggaggtaca gggtgatttg tccatgctct ttgacaagca atatatcctg acagaaaacg
    12961 tcgtggtaga aacccgttac cgcaccgaaa ccgatacatg gacggacgcg gagggcaaca
    13021 cccatacaga cacctaccaa gtgccgtatg actattacat ctgctacgtc acgctggaaa
    13081 acttcgacct gtcccatgtg ccagtctata tcatggacga ggaaacgcta agtttctatt
    13141 ccctgtatat gtccacgcta ggaaaccggc ctgatctgtt ttccggcagc cagtacccca
    13201 acgcctccac cctgacgccg ccgacctact acgacgtacc cccggaggct ttggaggatg
    13261 ccacctttgc cgccatgctc aaagaggcgg aaaaatatat cggttatccc tatgtgtggg
    13321 gcggcagctc tcccagcacc agctttgatt gcagcggctt catttcgtgg gtaatcaatc
    13381 atagtggatg gaacgtggga cggctggggg cgcagggcct atataacatc tgcacccctg
    13441 tttccagttc gcaggccaag cccggcgacc ttgtattttt caagggaacc tacgacaccc
    13501 ccggcgtgtc ccacgtcggc ctgtatgtgg gcaacaatgt gatgctgcat tgcgggaacc
    13561 cgatttccta cacgaacctt acttcaaact actggcaatc ccatttttac agttacgggc
    13621 gtttacctta acgccagaaa ggagctttca attatggcaa ccaaacttga ccgcatcgaa
    13681 aaggacatcc agaaaaccaa gtctaagatc gcggagtttc agaaacagct tcgggagctg
    13741 gaaacgcaga aaaccgaaca ggagaatttg cagatcatcc agcttgtgcg cggcatgaac
    13801 atgaagccgg aggaatttgc cgcgttctta cgcagcggcg caatgcaggc cgcccctgcc
    13861 gcaacccctt accatgaaca ggaggacaat gcccatgaag aataagacca gacggatttc
    13921 ccgtattctc cccgcacttc tggccgtgat gctctgcgtc acggcctttc ccgttaccgc
    13981 ccttgcaggc ggcaatgacc cggcaccggc cccgctgccg gaggctacaa ccgaacccgc
    14041 tacgggcggt ttggagcctg aaacagacga aaccggcacc gccacggacg ggacagaaac
    14101 cgaggccgaa aaagataccg gccctctgac cggcaaggac atcaccgacc tgttttccgc
    14161 gctgttcggt tccaaggtga gcattgccgc cacggacgac ggcatccaga tcaccaccgg
    14221 caccgacaaa aaagaacctg aacagaccgg caccgtcacc accaacggcg gcaggctgaa
    14281 tgtccgcacc ggcgcggggc ttgacaagac cgcgttcacc cagcttccca atgggacgac
    14341 ggttgaggta gtcggcacag acggcgactg gattaaaatt ctcttgccgg agcgcatcgg
    14401 ctatgtccat tccgactata tgaccgtcag cgaaaaggaa gtagcggcta ccggcgaggg
    14461 cggtttttcc ctctccattg acccggagga aattgcttct ctgctggaac tgttcaatgg
    14521 cggcggggcc agcgccgccc tgtccccgga cgggaacctg tcactgattg acgacatcgg
    14581 cagcgccgcg aaaaatggca agcagtttat caccgtggaa accaaaaacg gcaatgtgtt
    14641 ttatctgatt attgaccggg acgacgaggg aaaagaaact gtccatttct taaatcaggt
    14701 ggatgaagcc gatctgctga cgctgatgga ggacggggaa accgtcaccc ccgctgctgt
    14761 ctgctcctgt accacgaaat gcaaggccgg ggctgtgaac acgaattgcc cggtatgtaa
    14821 aaccaacctg accgagtgca gcggcccgga gccgcaggaa ccccagccgg aggaacccga
    14881 agccccggag gaagaaccca agggcggcgc gggcggcctg atcgtgttcc tgctggtggc
    14941 gctggcgggc ggcggtgcgg ccctttacta tttcaagttc aaaaagccca aggctgaaac
    15001 caccggcagc gacgatttgg gcgagtatga ttttggggag gacgaggatt tggaggacga
    15061 ggaacccgaa accgagtttg acccggaagc tgattttgtg agtgaacagg aggacgagga
    15121 atgacatata aacttgtaat cgcggaaaaa cccagcgtgg cccgcagcat tgcgggcgtg
    15181 atcggggcca cggagaaaca cgacggctat ttggagggga acggctatct ggtgagctgg
    15241 tgcatcggac atctggtgtc ctttgccgac gccggacgct atgacgagcg tttcaaaaaa
    15301 tggcgctatg aggatttgcc cattctcccg gaaacatggc agtacatcgt ccccgacgac
    15361 aagaaacagc agtttgacgt tctctgttcg ctgatgcagc gccccgacgt gaccggcctt
    15421 gtgtgcgcca ccgacgccgg gcgcgagggg gaattgattt tccgtttcgt ctaccagatg
    15481 gcgggatgcc aaaagccctt tgagcgcctt tggatttcct ctatggagga cagtgctatt
    15541 aaggacggct ttgcccacct gaaaccgggc ggcgactatg atccgctgta tcaatcggcc
    15601 ctgtgccggg ccaaggccga ctggctgatc ggcattaacg ccacccggct tttttccgtc
    15661 ctgtaccaca agaacctgac tgtgggccgc gtccagacgc cgaccttaaa aatgctggtg
    15721 gacagggacg ccaagattac cgatttcaag aaagagaaat atcacatcgt ccatatcacg
    15781 gcgggcggcg cggacgcggt aagcagccgc ttttccgacg ctgccgaggc aaacaccgtc
    15841 aaggcggctt gtgcaaaagc gcaggccgtt tgcgtttccg tgacgcggga gaaaaagacg
    15901 gaacagccgc ccaagctcta tgaccttacc accttgcagc gggaggctaa ccggcttttc
    15961 ggctataccg ccaagcagac ccttgactat gcccaggcac tgtatctaaa gaagctgctg
    16021 acctatccga gaacggatag tcaatatctt accgacgata tgctgcccgc cgtggaaagc
    16081 cttgtgtccg gcctgtggga gctggtgccg tttgccaagg gcctgaacct ttccccgcag
    16141 tttgaccgca tcctgaacag caaaaaggtg tccgaccacc acgccattat ccccaccgct
    16201 gaatttgtga agcagggctt tggcactctg gccgagagtg aaaaaaagct gctgtccctg
    16261 atctgctgca agctcttatg tgccgtggcc gggccgcata tctatgaggc cgtcaccgcc
    16321 acatttacct gtgccgggca ggagtttacc gccaagggca agaccgtcct ttcccccggc
    16381 tggaaagaga ttgaacgccg gttccgttct tccttaaaga ccgacgccga cgaggacgcc
    16441 gaggcggtgc gggagctgcc ggagctttgc gagggccaga cctttgcaga cgtggccgcc
    16501 tctgtcaccg agcatttcac cacgccgccc aagccctaca ccgaagatac gcttttgtcc
    16561 gctatggaac gggccggggc cgaggatatg ccggaggatg ccgagcgcaa gggattaggc
    16621 acaccggcca cccgcgccgc cattctggaa aagctggtgc agatgggctt tgtccagaga
    16681 aaaggcaagc agctcattcc caccaaggac ggcatcaatc tggctgtggt gctgccggag
    16741 gcgcttactt ctccccagct tactgccgaa tgggaaaacc ggctgaccga gatcgccaag
    16801 gggcaggccg atcctgacga atttatggac ggcatcgaga cgcaggcgcg ggagctggtg
    16861 cagacctatt cctgtatcag tgaggaaaag cagaaattgt ttcaggccga gcgtgttcct
    16921 atcgggacgt gtccccgctg tggtgaggct gtctatgagg gcaagaaaaa ctactactgc
    16981 ggaaaccggg cctgccagtt cgtcatgtgg aagaatgacc gattttttga ggaacgcaag
    17041 aaagcgttca ccccgaagat cgccgctgcg ctgctcaaaa ccggcaaagt caaggtaaag
    17101 ggcctctatt ccgtaaagac cggcaaaacc tacgacggga cggtgctttt ggctgatacc
    17161 gggggcaagt acgtcaacta ccgcattgaa cacaggagct aaaggacagt aacacagcaa
    17221 gcatagggag tgtggctaca ctcccggtaa aatcccccgg ccgcgtccct gccgggccgg
    17281 ggatttttgc ttgttgggga agtccccaac cccctatttt ttgaaacaga acagagaaag
    17341 gagtgaccgc gtgaacgaag tattttctat ccaagtccat aaccggcagt ttttggaaca
    17401 gggcaaacaa ggcgtctggc tggatttgcc caccacaacc gaaacgctgc aagccgccct
    17461 gcgggatgtt ggcattactg ccgacaatcc gcaggatttt tttatcaacg ggtattcctg
    17521 cccggaggac agacaccttg ccattcccta tgacatggtg ctggccgccg acgtggacga
    17581 gctgaatttc cttgccgccc ggctgggcca gcttgacgcc gccgagatcg cagagctgaa
    17641 cgccgccctg caaaatcccc gtggcggctt tgagaatatc ggccagatca tcgactacac
    17701 cgaaaatgtg gattactatg tgcatcttcc cgacgttagg ggggatggac agttgggcga
    17761 ctactacctg aaccgttccg gcatggtgga tatgccggag gaatggaaag ccgggattga
    17821 tacggcgcag tttggacgcc acattgccca gcaggaacaa ggcgttttta cccagtacgg
    17881 ctatctggtg agaagcggcg acgaatggca gcgcgtccat gagggccagc cggtgccgga
    17941 ggaataccgg gtgatgcact acccccagcc ggaagctgac cgggaggaac cgcaggccca
    18001 gcagccggag gcatccgaaa gccccgacaa agcgccgccc ccggttatcc cgatcatgct
    18061 ggacggcatg aacagcaaag aacgcatgaa agtgattact gaccgtctgg aaaccggcgt
    18121 acaggagctt tttgaaagta gccgctatat ggcttactta tccgctatgt ccaagatgca
    18181 cgattacagt ctgaacaata cgctgctgat cgccatgcag ggcggttcga ttgtggcggg
    18241 ctataacagg tggcaggatg aatttgaccg ccatgtgaag cggggcgaga aaggcattaa
    18301 gatactggcc ccggccccgt tcaaggttaa gcagcagatg gagaaaaccg acccggccac
    18361 ccagcagccc gtgatcggcg cggacggcaa gcccgtgacc gaagaacagg aagtgacggt
    18421 tccggccttt aaggtggtga cagtctttga catcagccag accgagggca aggattttcc
    18481 catgccctat atcagtgaac ttaccggcga cgtgcagcag tatcaggatt tctttgccgc
    18541 gctggaacgg acatcaccct atcccataag cattgagccg atttcagacg gggccaaggg
    18601 ccgctgcttc tacgcaaaac agcgcattgc catcaacgag ggcatgagcg aattgcagac
    18661 cctaaagacc gccatccatg agattgccca tgccaagctc cacgccattg accgggaggc
    18721 ccccgccgag gaacaggcgg cccggccaga ccgcagcacc cgcgaggtac aggccgaaag
    18781 cgtggcctat accgtctgcc agtattacgg gctggacacg tcggactatt cttttggcta
    18841 tgtggccgat tggagcagcg gcagggagtt atccgagctg aaagcctccc ttgaaaccat
    18901 ccgggccacg gccagcgaat tgataaccga gatcgacggc cattttgccc agcttcagca
    18961 ggagcgcgag gcccggcagg aacaggaggc cgtcacggtt gacgttaccc cggaggcagc
    19021 ggccccggcg caggccgagg aagaacaggc cccggccctt gatccggcag ccgagccgct
    19081 tgtgactgtg ctatggagcg aaagccccca gctaaaggac ggccagcaaa tgccgttgca
    19141 tgaggccgac gcgctgtttg cccgtctgga tgcagcatat ccggcagagg caggctatga
    19201 caaaaccgcg ttccgcattg actgtatctt tcagggcgag ccggacagct acgaggggcg
    19261 gcaggatttt ggagataggg acggttccct gatcgaacat atccagagct accaccaatt
    19321 ttacgcgcag gacgaaaact ggaaaaagca tgtaatcgct acaaccggca tagagggctg
    19381 ggagcaggac aaggcccatc gggatatgct gttaggcgag tttgtcccct atatgaagct
    19441 gcattgcagc cttgcccaaa tggaacagac ggcgcagggc cgcttacagt ccggggaaac
    19501 cctgacgccg gaggaaacgg cctatttggg ggcgcttctc tcttatgtgc aggagtgccg
    19561 ccccatgctc aatcagggcc agtatcagtt gccggagccg ccgaaactgg ctgacttcga
    19621 ccagagcttg caggactaca aagcacaggt acaggccgag atcgcgcagg aggccgccgc
    19681 agccggtatg accgtggagg aatacgcggc ggctggctat gaagcacccc aacaggacac
    19741 cttttccatc taccagcttc gaggcgggga ggaaacccgc gactatcggt ttgagccata
    19801 cgagcgttta caggccgcag ggctggccgt ggacagtgcc aactatgacc ttgtttatac
    19861 ggcaccgctg gataaaagca cgtctttgga ggatatttac cgcacattca acatcgacca
    19921 cccggcagat tttaggggac acagcctttc cgtttccgac gtggtggtgc tgcaccagaa
    19981 tggacaggac accgcccact atgtagacag cttcggctat atggaagtgc cggagttttt
    20041 gcgggaaaat cccctgcgta ccgccgaact gtctacggag cagaacgaaa acatgattga
    20101 cggcattatc aacaatacgc cgcccgaacc gcagccggag gaacccgaac agctatcttt
    20161 tgaggatgtg gcagttgctt ccggccagac acaggaggaa cccccggagg ctgcgccccc
    20221 gcaaacggct gtccgctatt atcccatcaa tgagggagcc gcccgccgcg ccaaggaagc
    20281 catgagcttt tctgactacc ggcccggcag cgccacggcg gaataccggc actatgtcga
    20341 tcaggccgtg gaaattgcgg agcgccagaa aaagcgtgtg gagccagagt atcacgaaaa
    20401 gatagacagc ctccttgaca cctacgcccg caagctggcc gagaacatga ataagggcta
    20461 tgagattacc gcccgcgtcc cctctgtcct gattgccggg ccgtccaatt tcccggtacg
    20521 ggcgaaagaa aagcagaacg ccgccagtga ccgcaacatg gaggaatttc agtacattca
    20581 gggcttgctt gacaaaatcc gcagcaccgg catgggtggt atcagcgccg acgatccgca
    20641 ggccgtttcc aagctggaaa agaaactgga aaagctggaa gcgtcgcagg agttgatgaa
    20701 ggccgtcaat gcctactacc gcaagcatgg gacgctggac ggatgccccc atctgatcga
    20761 aagaggcatt gaaaacctga aagcggatat ggcgtcgggc tggcactatg agaaaaaacc
    20821 gttccagtcc tggcagctct ccaacaataa cgccgagata cgccggttaa agggccgcat
    20881 tgaggaattg acccgccaaa aggaggcggc ctatgtggga tgggagtttg acggcggcac
    20941 tgtggagatc aaccgggtgg ccaaccgcct gcaaatcttc tttgagggga agccggatgc
    21001 tgctgtacgc gacgagctga aaagcaacgg tttccgctgg tcgcccaagg ccggggcatg
    21061 gcagcggcag ttaaacgaca ccacaatccg cgtcgctgac cgtatcaagt gcatccagcc
    21121 cctttccggc gaaaagccca gcgccttaca gcttgcggcc cgcagggaga aagaaaagcc
    21181 ctctatccgg gcgcagctca acgcggccaa gaccgaacag gccaagcagc caccggcgcg
    21241 ggaaaaatca aagggattgg aggtagagta aatgcagaag tttgaaaatg tggacgttct
    21301 ggccgcgctg gaacagatca tgcggcagaa caccgcgttt tatcaaagtg actttggcat
    21361 tgacaaagac attatcaggc gggatgccgc cagcgatcag gccgcagaca agaccctgct
    21421 ctggatgtcc cggccctccg ggacgtactg tttccgggaa cgtgatgttt tcctgaaaga
    21481 cacccgacag tacaacacat ggaaatttta cggggagcag acccgtgaca agatactggc
    21541 ctacgcggtg gagctgaccg gcacacaggg cggcactatc cggggcaatc tctatgagct
    21601 ggactatccg cagcatttcc ggcacgtcat tgaaaccgcc cagccggtga gtgtgaaccg
    21661 gctcttttat gagcatggca cccgtgacat cccggagagc cagcgttttg acggaagccc
    21721 tgaccgggta ttgggaaact ttttgtacta tgaggcccag ccccatgacc cggctgtgct
    21781 gcaggaggcg ctacggacgg agcagcacag ccgggggcgg gccgtccccg gcgatttcaa
    21841 agtccacgtc accgctttgc atgacagccg gattgaaacc gaggcccggc gtattgtgga
    21901 cagcctgaaa gatctgtccg cgcctaacag ccccaataaa acgcatttca tggtggagat
    21961 ctccccgcac tttgcggcga ttgcttccac caaggataat gaccggctct ttgccatgct
    22021 gccctataaa tccctgtgtt ttaccgggat gaaaggcagg catggcgtgt atgccgtgat
    22081 taaccgggac gagcgccggg atgtgagtat tcgcaggccc cggccctcta tccgcaagca
    22141 gctatccgac agcaaacagg ccgccagccc aaaaaaggcg gcggccagaa ccaaaaagaa
    22201 tgaattggag gtataaggaa tgatgcactt taccgtggag gaagaaaacc tgatctgtat
    22261 gtaccacaat gcagaccggc gcagaacgat aaacaggctc accgccgtca tgccggttat
    22321 ggatggggat atgcgggcgc aggcccagca gaccctttcc aagctggaac gcatgacgga
    22381 tgccgactat gacggccaga agttccactt cacagacgaa taacgaacag cagacgccgg
    22441 gcgcggcggc catgtggtcg cttgcgtccg gcgtcttttc tttttgccta ttcctacgca
    22501 tttagaaccc ggtattgtcg gggataggat gaagtattcg cttgcccctg caaacatgcg
    22561 ggagaagcct tgcagcgggc ggttttcaat cccttaaatg cgtaggttgc agctataatt
    22621 agacccctga aacagatata gccgcgcagc gggtgtatct gtttcagggg tgtgcaggtg
    22681 atagccgcaa agcggcgtaa gagggtgcag ccctcttgcc gtctatctct gttccagcgg
    22741 gaagctcggc aatgcttcca gcagtttcag ggaaatgcgc tgccgtatat cttcgtcaat
    22801 gactttcttc tttttgccgt ttgcctgtct gatctccacc gtggaaagct cgttgatgta
    22861 gtcagcataa tgggccaaca ctttttccgt ggcccacctc tcaccggcaa cggcggcttt
    22921 gatcgtttca tagtccagca gcttttcttg tccgtccatc ctgatacccc cgtcagactg
    22981 attttttcta tgataccata ttcggaagca tggaaccaga gaaaacggcg cttttctgct
    23041 ggtaaattct acgcatttag aacagcactt cggcggggat aggataaaac acccacatac
    23101 cccgctgacc atgccggaaa agccttgaaa tataagggct tttacagctc taagtgcgta
    23161 ggtttccagt atcttttccg ctggcgctcg gcctctttgc ggcggcgcat ccgtttggag
    23221 caggccgcgc agtatttggc ccggttagac ttcggcgtga acgtggcccc gcactcacag
    23281 cagcgtttca cttcgtcccg gtctttggtg atctcggcat acagggccgc atccagcggg
    23341 aggacagcgg ccttaaacca gcggcacaga agcgaatagg aaatgctctg tacgcaaata
    23401 cattcttcgc cgtcgtccag caacaggcag ttgccgtcac aatagttgca gcactcatgc
    23461 gtcagcttcc gggccttgcg atattgaggg taattcattc gtggaatact catagctctt
    23521 gcgtccattc ctttcccggc gtcatacgca aaatgccgtc cacattctgc ttgatgatgc
    23581 cgtattcctg catctgcttt ttcgcttcgc cgtacttctc atacagccgt tccttttcct
    23641 cggccaatcg cttgtactcg gctttcaggg cgtacagatc ggggagcttt ttcacaccct
    23701 gtccctttag ggcctttgcc gcagcttcat acaaaatcaa cgtgctttca tgctcacgcc
    23761 ggaattttgc cttatccgct gcattgcggt attccatagc cacgggccag agctgcttgt
    23821 aggtagaaat attctttatc agcaccgcca tatccgcaag ccggtgttcc atagctttga
    23881 gggtagcggc ggcctcgtcg ctggcggcgg tgatctcggc cactttgctt tccaaatccg
    23941 ggtaggtgtc tatcccatgt tcagtcagga aattcaaagt ctttgcggcc tgcttcaaat
    24001 tgtggagctt cgcccacctc tcatagcctg ccgactgctg ggccttaatg ttgttttcca
    24061 gatcaatccg caaagaaatc cccttgcgct ctttgggcgc tctggtgata acgcggcctt
    24121 tgatacgctc tttgatggct tcctcggtgt aggcttcccc caatgtcttg cagcgggtaa
    24181 agcgttcctg tccgggagcg cggaaagaaa taaactttcc cggcttgatc tcatagccct
    24241 gcgcctgcat gagccgcagg aaatcatcaa aatcttttgc ctgcgggata gcggcgtcaa
    24301 tggctgcttt gagcttcgct ttccagctcg tccctttccg ctgggcgtcc cactcggcat
    24361 agctcttgcc cttactctgg ccgggcttca ccacggacaa tccgttttcc ttgcacagcc
    24421 tgtcgctggt gcgccggata taggcatagc tctgtttgtt ggaaatgtac ttgcgctgat
    24481 ttaccatatc cacggcacag aaaataatgt gattgtggac gtggccctta tcaatatggg
    24541 tagtcagcac atagggatat ttcccttgca gcacttcctc ggcaagctgc ttcccgatct
    24601 catgggcctg ttccggcgtg gcttcgccgg gggcaaagga ttgtatcagg tggtggccca
    24661 gattattgcc cttttggtat gcctgcgcca gcagcatttc aaattcaatg tccgctgttt
    24721 cataagagca ggcaaaggag gccaccagca gcttgccgtc agttttgtcc gggttctcga
    24781 tgtagtcaag ggcttttttc agcgttgact tgataggatg tatctttgta actgccatat
    24841 ctgcgccagc gcccccttta tatcctcgat gtcctgtgcg tacaccggcc ccataccgtt
    24901 cagcctcttt gcaatctgat tgacgttgac gccgatcttc tgtatctcgg cggtctgcgc
    24961 ccggatgtcg ctggtgtcca tgttgacgat atagccgtca atgagcatct tccgggcgta
    25021 ggctgaaaag ttcttcgtat gcagcagcgc cattttctgt tcaatcatag cccgttcctc
    25081 cggcgtcaca gggacgcgca gcacgatttt tcgctttctg tttgccatgc ttcaccgtcc
    25141 tttccattca ggggtttggg gatttcccca acaagcaaaa cggacgcggc ccggcaggga
    25201 cggggcaggc cgtttttccg ggagtggcta cactccctat gcttgctacg ttactgtttc
    25261 tccactaaca agacgtttcc aaagccccaa actacccgca aaatagccct ctggaaagaa
    25321 aaaagtgcaa aaaaatagaa gctacgctgt gcagctcccg ctaaaagtaa aacagaggat
    25381 gccagttaca catcctccgt ttcattcttc ttcggctttt caattagtat ggcgctgtca
    25441 atcgcgccct cgatgatcgg caggtattct tcggggcaga gtttcagctt gtggctgacc
    25501 ctttgccgca gttcgctttc ctcccgcatg atctccgggt taaagtagcg ttccacagga
    25561 agcccgcagg ctttaataag ctgtatgaca accggcagac tgggaatggc ccgtcgtttt
    25621 cgatgttggc aagatagcgc cagtcaatat ttattttctc tgccattgct ttgcgcgaaa
    25681 cgcgctttgc ttgccgggcc gctttcacat ccgcgccgaa agtttcaaag ccgggacaat
    25741 cttcaatatt cgccatatcg cacctcccac ttacattgta tatttcatac tctgcccgtg
    25801 gaatgttggt atatgcacct ttcttaaagt ttatatttca cattttgttt cttttattct
    25861 tcgagcaaaa gaattataat gtaccctgtg gaggtgtact atggactaca tgacgttgaa
    25921 agaggcaggc gagaaatggg gtgtgccccc acgatggata aactactatt gtgctgcggg
    25981 acgcatcccc ggcgctgtaa agatggcgac gatctggctc attcccaaaa ctgcggaaaa
    26041 gccgattgac aggcgtacaa agaaaggaaa ggcggttact gaatagtata atgaaaccgg
    26101 cgcattttgg atatggtttt gaagagagag aaagcatggt attttgtcac ggaaaaacta
    26161 taagatgata gatggcaggc tgctccagac cgataaaaag tattctcatc tgaaacagaa
    26221 agaaaagatt tacacatgga tgtatgaaga agcgaaactt tatcatgaca gaaatggaaa
    26281 atgcccgaaa caaagacgag gaagctgctg ctgtatcagc cgtatatggt agaattgaaa
    26341 aggccggcat ctggattctt tatggtgaag tctccagaca ttggataact gctggtctaa
    26401 tgcgcctttg agtatggggt gattgatttt aataccgggg aggtacggct ttttgaggaa
    26461 aatattctgg gatttacatt tggcgattat tacatttcct gtaactgcaa ggacacggat
    26521 gatgaagata tgaggaatat agacttgctg attttctact gtcttgaacg gtattaggac
    26581 tgaatctgct gcgcggagtg cggcaagagc gaccggaggg agtacacaca ggccctgcct
    26641 gtgtgaaaag ccataccgca aggtgcattc gcaaaaaaga ggccgcgcat tttgctgagg
    26701 cttggggtgt ccccaacaag caaaacgaga acgccgcagg cgggcaagtg gtggatcaga
    26761 atatccgtaa aggatattct gatccattgc ttgccagtat tatcgggatg ttttgacaac
    26821 cgctgtaatc gaaacatccc aaacattaag aaggtgttaa ttattattat cttgatttaa
    26881 ttcgtttaaa ccaataatat agatacatac accatgaaaa gaagcgagta tcccaatgac
    26941 ggatgattaa aatccgaaaa gaaatcgctt tgaaactaca gggaaactac agactgttat
    27001 gttatcttct taaaatggag ggatttttat gtcgatacga attctacttg tcgaggatga
    27061 tgatcatatc tgtaatacag taagggcgtt tctggctgag gcaggatatc aggtggatgc
    27121 ctgcacagat ggaaatgagg catacaccaa gttttacgaa aacacttatc aactggttat
    27181 tcttgatatt atgctgcccg gtatgaacgg gcatgaactt ttgcgtgaat ttcgtgcgaa
    27241 aaatgatact cccattctga tgatgacagc cctgtcggat gacgaaaacc aaatccgggc
    27301 gtttgatgca gaggcagacg actatgtaac aaagccattc aagatgcaga ttttactaaa
    27361 gcgggtggaa gccctgctgc ggcgcagcgg tgcgctggca aaggaaatcc gtgtcggcag
    27421 gctgacactt ctgccggagg atttcacggt actttgtgac ggtacagagc tgcccctgac
    27481 acgaaaagaa tttgaaatcc ttttgctgct ggtgcagaac aaaggcagaa ccttaacgca
    27541 tgaaatcatt ttgtcacgta tatggggata tgactttgag ggtgatggca gcacagtcca
    27601 cactcatatc aaaaatttgc gggccaagct gccggaaaat atcatcaaaa ccattcgcgg
    27661 tgtaggatac cgattggagg aatcataata atggaaagaa aagggatttt cattaaggtt
    27721 ttttcctata cgatcattgt cctgctactg cttgtcggtg taacagcaac actgtttgca
    27781 cagcaatttg tgtcttattt cagagtgatg gaattacagc aaacagtaaa atcctatcag
    27841 ccattggtgg aactgattca gaatagcgat aggcttgata ttcaagaggt ggcagggctg
    27901 tttcactaca ataaccaatc ctttgagttt tatattgaag ataaagaggg aagcgtactc
    27961 tatgccacac caaatgccaa tacatcaaat agttttagac ccaactttct ttatgtggta
    28021 catagagatg ataatatttc gattgttgct caaagcaagg caggtgtggg attgctttat
    28081 caagggctga caattcgggg aattgttatg attgcgataa tggttgtatt cagcctttta
    28141 tgcgcgtata tctttgcgcg gcaaatgaca acgccgatca aagccttagc ggacagtgcg
    28201 aataaaatgg caaacctgaa agatgtaccg ccgccgctgg agcgaaagga tgagcttggc
    28261 gcactggctc atgatatgca ttccatgtat gtcaggctga aagaaaccat cgcaaggctg
    28321 gaggatgaaa tcgcaaggga acatgagttg gaggaaacac agcgatattt ctttgcggca
    28381 gcttctcatg agctaaaaac gcccatcgcg gctacaagcg ttctgttgga gggaatgctt
    28441 gaaaatatcg gtgactacaa agatcattct aagtatctgc gcgaatgcat caaaatgatg
    28501 gataggcagg gcaaaatcat ttccgaaata ctggagcttg tcagcctgaa tgatgggaga
    28561 atcgtaccca tagctgaacc gttggacata gggcgcacgg ttgccgagtt gctgcccgat
    28621 tttcaaacct tggcagaggc aaacaaccag cggttcgtca cagatattcc agccgggcaa
    28681 attgtcctgt ccgatccgag gctgctccaa aaggcactat ccaatgtcat attgaatgca
    28741 gttcagaaca cgccgcaggg aggcgaggta cggatatgga gtgagcctgg tgctgaaaaa
    28801 tgccgccttt ttgttttgaa catgggcgtt cacattgatg atactgcgct tccaaggctg
    28861 ttcaccccat tctatcgcat tgatcaggcg cgaagcagaa aaagtgggcg aagcggttta
    28921 ggacttgcca tcgtacaaaa aacgctggat gccatgagcc tccagtatgc gctggaaaac
    28981 acctcggatg gcgttttgtt ctggctggat ttaccgctca catcaacatt gtaaatattt
    29041 aaaacttaaa tgat
//
LOCUS       DAZBSA010000027        28836 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000027 DAZBSA010000000
VERSION     DAZBSA010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.027"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            21..542
                     /locus_tag="ACHGMT_001075"
     CDS             21..542
                     /locus_tag="ACHGMT_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN6834838.1"
                     /translation="MHIGDKIHKLRRLNNETMNDLVKQTGLSKSWVSDIESGKKQRAD
                     FNKICAIAKHYKVSLEYFQDDYLDSDEHEDIIPLTDETRVKSVKSLEQSLKYLNNKQE
                     PNLLEIKLKDTDSVPEVYYKGQKLEGLIDVSYHYHTTTDVVAGSTNDINMEYLGEVTK
                     YPDIKTIGHKRDV"
     gene            639..860
                     /locus_tag="ACHGMT_001076"
     CDS             639..860
                     /locus_tag="ACHGMT_001076"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFN6834839.1"
                     /translation="MCQECKRRGRNTRGTIIHHIVEAREDLSLFWSVDNLECICVACH
                     NREHPERSGGKKKPKPKSHIVKMYSTPER"
     gene            complement(1017..1181)
                     /locus_tag="ACHGMT_001077"
     CDS             complement(1017..1181)
                     /locus_tag="ACHGMT_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HFN6834840.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGREVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            1284..1805
                     /locus_tag="ACHGMT_001078"
     CDS             1284..1805
                     /locus_tag="ACHGMT_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFN6834841.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            1792..2232
                     /locus_tag="ACHGMT_001079"
                     /pseudo
     CDS             1792..2232
                     /locus_tag="ACHGMT_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(2272..2430)
                     /locus_tag="ACHGMT_001080"
     CDS             complement(2272..2430)
                     /locus_tag="ACHGMT_001080"
                     /inference="COORDINATES: protein motif:HMM:NF024064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HFN6834842.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            2523..3284
                     /locus_tag="ACHGMT_001081"
     CDS             2523..3284
                     /locus_tag="ACHGMT_001081"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFN6834843.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            3471..4823
                     /locus_tag="ACHGMT_001082"
                     /pseudo
     CDS             3471..4823
                     /locus_tag="ACHGMT_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            4839..6059
                     /locus_tag="ACHGMT_001083"
     CDS             4839..6059
                     /locus_tag="ACHGMT_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFN6834844.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDAEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALIKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGGNEGLKVKFASNVDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGSNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTNSSKSVAENG"
     gene            6094..6771
                     /locus_tag="ACHGMT_001084"
     CDS             6094..6771
                     /locus_tag="ACHGMT_001084"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFN6834845.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGIDCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAETPQEETLLQKKLKA
                     LNGGKNE"
     gene            6768..7964
                     /locus_tag="ACHGMT_001085"
     CDS             6768..7964
                     /locus_tag="ACHGMT_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010905933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFN6834846.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRQFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     INPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSNWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIINVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTSIANE
                     VPVTNG"
     gene            7979..8302
                     /locus_tag="ACHGMT_001086"
     CDS             7979..8302
                     /locus_tag="ACHGMT_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFN6834847.1"
                     /translation="MILDPKMDLDEIKNALKIDTDDDDVEVRRAVQAAIAYIRGAIGN
                     DKPSFYKQENDTVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKARYL
                     LFVEE"
     gene            8306..8641
                     /locus_tag="ACHGMT_001087"
     CDS             8306..8641
                     /locus_tag="ACHGMT_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFN6834848.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVRTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            8638..9033
                     /locus_tag="ACHGMT_001088"
     CDS             8638..9033
                     /locus_tag="ACHGMT_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFN6834849.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEKLQQNT
                     PYWDGTKSNGKRGSYMQEHAKDHVVTSSVKNGLIEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            9030..9401
                     /locus_tag="ACHGMT_001089"
     CDS             9030..9401
                     /locus_tag="ACHGMT_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834850.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EIFGNYEDFASNNPLTIQFNVQVDVWVSTLKEVDAFYFALDEIMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            9417..9977
                     /locus_tag="ACHGMT_001090"
     CDS             9417..9977
                     /locus_tag="ACHGMT_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFN6834851.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGVNVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLAKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEEMTLQKFKDLVNNKAGE"
     gene            10104..10415
                     /locus_tag="ACHGMT_001091"
     CDS             10104..10415
                     /locus_tag="ACHGMT_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834852.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     R"
     gene            10439..10630
                     /locus_tag="ACHGMT_001092"
     CDS             10439..10630
                     /locus_tag="ACHGMT_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834853.1"
                     /translation="MDQIYKDLIESGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            10630..14475
                     /locus_tag="ACHGMT_001093"
     CDS             10630..14475
                     /locus_tag="ACHGMT_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFN6834854.1"
                     /translation="MAGKGQPAGNIKLGISLDSTNFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKKIEDFGDKSTRSARKLDGLKDKLGSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIDKVGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NKVSDAIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGADKLEEWKVPQYAKGTPNGGHPGGPMMVNDGRGAE
                     AVITPNGQAFIPRGRNVVLNAPKGTHVLTAEETAYMTGNKAPRYRYAKGTGFFGNLWN
                     NVKGFAGDVGNKLKDVVGDVWDFVTDPGALARKVLNGLGVLEGLVKYPLDVGKGILSK
                     ATEALTNKITELFSSGSLDTSMGMQGVYKYLADVAVAVMKKFPGFVATSGYRPGDPYS
                     HGKRNAIDIALPGVTGGSPRYTEAANYAFEKFANKIGYVITNGKVRDRSGQSGTGIHN
                     DWRPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTAN
                     LNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFDP
                     LSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAKR
                     SRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKGN
                     SRTDRDLALDIQKILVRRM"
     gene            14476..15348
                     /locus_tag="ACHGMT_001094"
     CDS             14476..15348
                     /locus_tag="ACHGMT_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein domain-containing
                     protein"
                     /protein_id="HFN6834855.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSENVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            15345..16394
                     /locus_tag="ACHGMT_001095"
     CDS             15345..16394
                     /locus_tag="ACHGMT_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834856.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGADEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            16398..17222
                     /locus_tag="ACHGMT_001096"
     CDS             16398..17222
                     /locus_tag="ACHGMT_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834857.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVTSTGSTVSISKISDSYELTLS
                     KGEILWSGTALMHDTQTVQPSKKMWQTVSGFLLVWLPYENGQAIGDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            17240..19372
                     /locus_tag="ACHGMT_001097"
     CDS             17240..19372
                     /locus_tag="ACHGMT_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFN6834858.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKTNDRIDQTNQQIGDLGKLKKMYSNSIDFGDYD
                     YSGNPNLSAKLNASSFSLGTGATVADDNDEIVFTLDGTNQLSKYASKAQVPVAEGKQY
                     TISCEIMLEDGFTGDPSGIRLQHAYLPGGVVVLQTETVPKNELNTWQTLIGTRTVKYT
                     SDVPNEWYPLFRDIRTLKPTGKVRLRKIKIEEGSTATPYQPNLLDDPYWLGKAPLGEN
                     IANKSVTFPIKSSAYSLYQANMEEEFVLGQTYTITMKATKPPIQTFIVYNEDSGDYRY
                     GNLEPVEGLVDTWGLTFTPQKVGVNYPKRLTIIQYPQSTTGACQIDWLKIEKGNTRTP
                     NISEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEI
                     NSQLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSA
                     KLYREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQL
                     SIGADGAIKWRRNSSYGSDYTFAITYTI"
     gene            19389..19538
                     /locus_tag="ACHGMT_001098"
     CDS             19389..19538
                     /locus_tag="ACHGMT_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834859.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPILNWKPKFNY
                     WEGNK"
     gene            19535..19981
                     /locus_tag="ACHGMT_001099"
     CDS             19535..19981
                     /locus_tag="ACHGMT_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834860.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            19983..20120
                     /locus_tag="ACHGMT_001100"
     CDS             19983..20120
                     /locus_tag="ACHGMT_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFN6834861.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIISKEESA
                     S"
     gene            20157..20450
                     /locus_tag="ACHGMT_001101"
     CDS             20157..20450
                     /locus_tag="ACHGMT_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834862.1"
                     /translation="MINLGEWGTIAGSITAIVSLILLVIRPVTASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERSR"
     gene            20473..20670
                     /locus_tag="ACHGMT_001102"
     CDS             20473..20670
                     /locus_tag="ACHGMT_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFN6834863.1"
                     /translation="MILPDKYYQVIKWTVLTVLPALSVLVATLGKAYGWNGTDMAVLT
                     INAAATFLGVITGVSAYNLKK"
     gene            20681..21700
                     /locus_tag="ACHGMT_001103"
     CDS             20681..21700
                     /locus_tag="ACHGMT_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFN6834864.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGVNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPQR"
     gene            21957..22604
                     /locus_tag="ACHGMT_001104"
     CDS             21957..22604
                     /locus_tag="ACHGMT_001104"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFN6834865.1"
                     /translation="MPNKNYRVYKRGTIVYVDFGVNVGYELSGNHFAIVLNNKDNSKN
                     GVVCVVPISSKKKSNYVSVGKILEVASIKQFISQADKLKEKLRIIILLSLNRGIIDES
                     DLNSFIKDIEAKGEKLSVEEVEKRAVKHNLVCETREQVIKEIKSLSEDMVKYQKVFNC
                     YKKYTQESYVMPLNIQTISKNRIRRINKFDPSGNMKAPGNVLNEVDESLKKYFTK"
     gene            complement(22788..24089)
                     /locus_tag="ACHGMT_001105"
     CDS             complement(22788..24089)
                     /locus_tag="ACHGMT_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFN6834866.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            24368..25174
                     /locus_tag="ACHGMT_001106"
     CDS             24368..25174
                     /locus_tag="ACHGMT_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFN6834867.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            25192..25899
                     /locus_tag="ACHGMT_001107"
     CDS             25192..25899
                     /locus_tag="ACHGMT_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFN6834868.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            25896..26867
                     /locus_tag="ACHGMT_001108"
     CDS             25896..26867
                     /locus_tag="ACHGMT_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFN6834869.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            26860..27889
                     /gene="vanB"
                     /locus_tag="ACHGMT_001109"
                     /pseudo
     CDS             26860..27889
                     /gene="vanB"
                     /locus_tag="ACHGMT_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
     gene            27895..28503
                     /locus_tag="ACHGMT_001110"
     CDS             27895..28503
                     /locus_tag="ACHGMT_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFN6834870.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(28569..28742)
                     /locus_tag="ACHGMT_001111"
     CDS             complement(28569..28742)
                     /locus_tag="ACHGMT_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834871.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         9667 a         5083 c         6549 g         7537 t
ORIGIN      
        1 tggattaagg aggaagagta atgcatatag gagataagat acataaattg agacggctta
       61 ataacgaaac aatgaacgac ttagttaaac aaaccggact gtcaaaatca tgggtatctg
      121 atattgagtc tggaaaaaag caacgcgctg actttaataa gatatgtgca atagccaaac
      181 attataaagt tagcttagaa tactttcagg atgattattt ggatagtgat gaacacgagg
      241 atattattcc tttgacagac gaaacaagag ttaaatcagt gaaatcgctt gaacaaagcc
      301 taaagtatct gaataacaaa caagaaccta accttctaga gatcaaacta aaagatactg
      361 actcagtacc agaggtttat tacaaaggtc agaagttaga gggattgata gatgtaagct
      421 atcactacca tacaacaact gatgtggtag ctggttcaac aaacgacatc aatatggagt
      481 acctaggtga agtaactaag tatccagata taaaaacaat cggacacaag agagatgtgt
      541 aaatgaaaga agctagacct agagacgaga tagacaaact atacaagacc aaacgatggc
      601 gagacctaag gcaagtagta atagctaggg acttcggcat gtgccaagag tgcaagcgtc
      661 gagggcggaa cacaaggggc acgatcatcc atcacatagt cgaggcgagg gaagacctgt
      721 cactgttctg gtccgtagat aaccttgaat gtatctgtgt agcttgtcac aacagagagc
      781 atccagagag atcaggcggg aagaagaaac caaaacctaa atcacatatc gttaaaatgt
      841 attcaactcc tgaaagataa gtttgcagcg aaatgaaggt agccccccta ctctaaaaga
      901 ttaaagagta aggcttgaga agaacggtgc tgtccttcct tcgtaaaaag accgcttttc
      961 aagttttttg gagaaaaagg aaaaagccga ccaatttaag ccggctttgg acgaagctat
     1021 ttcttagtcc atttgtttcc tttttgagaa gtaggaggta atcggtcgcc tggatcaatt
     1081 tttacttctc gtccgccttg gacatttcca ccacgaggtc ccacttcttt ataggttcct
     1141 tttggtttat tgtcttcgcc gggtttatag agttctccca taggaatccc tccctaaaaa
     1201 aatttcggca cagcactgcc gataacttaa ttataaggat tgtgataacg atttcaatat
     1261 atcttttgaa agaaggtgat attatgccgc aaccagcgaa gagtgcaaaa ttacaattat
     1321 taaacggaaa cccaaataag aagaataccg aagaactccg caagcgagcg gccgcagaag
     1381 acaaattaaa aatggctact gacaaaatca aaccgccgtt atggctagat tcgctaggaa
     1441 aggatacctt tgagtttatc gctgatgaat tgctgtctgt ggatttaatc agtaatccgg
     1501 acgtccatac aatggctctc tactccaatt ggtattcgca atacgtttct ttagaaaaac
     1561 agcttcgaaa actacaacga gagtacaagt tgaactatgc gcttgcgaaa aaggaggcag
     1621 aggcgagagg tgagccgttt aatgaaccta atgaattaat tggtaatccg ctctctcggc
     1681 agatggatac agcgtcgcgg aatctccgtt cttttggcgc tgatttagga ctatcaccag
     1741 cagccagagc taagttagct attaagatgg ctgatgatgg tggtgatgac gatgacgact
     1801 tctaatattt tggatatgtc ctacacagaa cgtgtggact attggcaaag ctatcttgag
     1861 gagcaagctt cttggggtgg ctttttaaaa tgtccatatc cggaattgtt aactacttgg
     1921 tatgcggaac gattaatcga tggaagcata ccagccagca aagaaaatat tcaagctgct
     1981 aaacggcata tgcgtgattt gcagcgccaa ggaacagatg attttccttg gatctttgac
     2041 gaagaaaaag gtcaccggcc tattagatat atcgaaaaaa aatgtaaacc aactgaaggc
     2101 gactttggtt cgtttgtttt gcaaccttgg cagcatttca taattggatc catgtacgga
     2161 tgggtacatc gtgatacagg agagcgtcgc ttccgcgagg ctcttatttt tgttggacgt
     2221 aaaaacggta agttttgccg tttaaaaatc gggcaaaatc ggtgaaaacc tttatttttg
     2281 gctttctttt agtaataatt cgatagcttt atcaagcaat ttagacatag gaaccatagt
     2341 ttcttccgac atttttttta atccttcgta taaatcttta tcaattgcgt tagaaattct
     2401 aattctgttt tttagtccgt atttgtccat gtgtgacact cctttttatt ttactataac
     2461 atccgattcc accgcttgca actaccatca attgatggta gtatataaaa gaggtgagta
     2521 gattgaaaaa atacattatt tacaaacact taaataaaat aaatggcaaa atttatatcg
     2581 gtgtcacaaa tgatattggc agaaggtgga ggagcggtgg gattgaatat aaaccaccaa
     2641 agaacgaaac ccaacaccac agaagttttt ggaatgcaat acaaaaatac gggtgggaaa
     2701 attttgatca tttgattatt gaagaagact taacgatgaa agaagctttt gagaaagaaa
     2761 agttttatat tgaactttat gactcaacta ataagaaaaa aggttataac atcgcaaaag
     2821 gcggaaatgg cggaataata tataaagttc accccaaagg aatgctaggg aaaaaacaat
     2881 ctaaagaatt ttcttcaaat cacagtaaat gggcaaagaa ccataagaat aattgtatga
     2941 caaacggtga tgttgtatgg ggtgttactc acgaacaccc aaaaggcatg ttgggaaagc
     3001 accacagcaa agaatcaatt atgaaaaaga aagcatatag tggtgaaaat gctgtaacct
     3061 caaaaccaat tgtcgcaatt gaacgggatg gaactaaaag agaatttcac agcgctaagc
     3121 tttgcatggc ttactattcg attagtacat gtgtttttta ccgactttta aaagatggcg
     3181 ccccttatgt gataaatcct aaagcgaact acagaaataa agaaaaaata ttagctatcg
     3241 aaggaattat gttcaaacat aaagaagata ccgaggtaag ttagtagatt gcgaaaggtt
     3301 actaactacc gtaacgcgta ggagttgaat aaatataatg ctcccaagag tgtccgacag
     3361 cagataattt gctgaaaatg tacgctaaac tgggccagaa ctgactgacc gatgaaaatg
     3421 agggtgacct ccagagtgcg agataaaaag ctcgcagata ataacaatcg aaaacatcac
     3481 taatttcagg cctttccaca tacatggtcg cttatgatga tgaacaaggc gccaacgttt
     3541 acgtattggc aaatgctcgt gatcaagcaa gcttgttgtt tgataaggcc gcagaaatgg
     3601 tcaaacaatc gccggcgctc tttaagaaat ttggtaagcc taaacgatca agtattaatt
     3661 atgctcccgc cttttctaaa atggaaccac gcgcctcaga tagccggaaa ttggatgggc
     3721 taaacactca ctttggtatt tttgacgaga tccacgagtt tacgaattac aatctgatca
     3781 acgttatcaa gaaatcaaga ggaaccagaa aacagcctct gatagtttat atcacaactg
     3841 ctggatatgt attagatggt ccgttgatgt cttattttga gcaaggtgtg gactgtttgg
     3901 aacatttgga agatgacatc gatgaacgga ctttctatta tctggcaaaa cttgacagtg
     3961 cggaagaggc tgatgaccca agattatgga tcaaagccaa tccgaatatt tgtctaatga
     4021 attttgttgg catgctagat gactatgtta aggataaaaa agatccaaaa gaatatgctg
     4081 actggattac caagcaattt aacttgtttt ccgatatcga tgagctgtca tttgtcgata
     4141 tgcctaccat taaacgaaac aataaaacca tcgatattga aacgctcaaa ggtaagaagt
     4201 gtgtcggtgg ttttgacttg tccgaaacgg aagactttac cgcagccgtt ttagaatttc
     4261 cgcttgaaac aggcgaggta ttcattttgc aacacacatg gatcccacaa gctagatttg
     4321 atcgagataa caatcaagag cgtatcaaag cgtgggagaa ggtgggagat ctaacgatta
     4381 ttcctggtga ttacgtcaat tatgaatacg tcttaaattg gtttgtagaa aattcgaaaa
     4441 tctatgacat tgtaaaaatc aattatgaca aggccaaggc gctacgatta aataaagaac
     4501 tagaaaatgc aggatttgaa accaacgaga ttcggcaagg gtttctatca ttaggtgggc
     4561 caatgcaaaa cttcaaggaa atgctattgg acggtaaggt gattttcaac aattccaagc
     4621 tttaccgatg gtatctatcc aacgtcaagc tggtgatgga tcgcaactca aactggatgc
     4681 cgtctaagca gtccaagagt agaaaaatag atggttttgc agcaagtttg aacagccacg
     4741 ccgaagtgtt gaatatgttg gttaatcctg tcggaaccgg gaaagtaacc tattactcga
     4801 tttccgattt aatgaatatg taagaaaggt gtggaggaat gagtatttta gatcgtttgc
     4861 gttcttttgg ccgagcgaag ccgaaagcga gcaaacaaga gtattttttg aatgacccgg
     4921 gattgatacc gtatttagtc ggaaaagatg aaatatcaga agggattttt tccgtaatta
     4981 gccgtgtatc gaacgttttt gcgtctctcc ctctcaaaat gatagatgcg gagtttggcc
     5041 aaccggacga ctgtcctgca tacaacttgt tgagcgaagg ccctcgatat tttacaaagt
     5101 ttgatttttt ccgggacgtg gaagttttga gaaactacca agggaatgcg tatgtgcaga
     5161 ttttccgaaa tatcaatgga gaagtagcag atatggcgtt aataaaacct ggtgcttgcc
     5221 atccagtgat tgatatggat agcggggagc tttactacca agtaactgcg actgacaaag
     5281 gcagttataa gcaagttatt tatgtacatt acatggaaat gctccacttt aaacaaccga
     5341 ggtttggtgg cttggaaggt acagacccca caaaagtatt aacgaatacc ctcggatatg
     5401 atcgagaagt ccgaaaaatc tctttaagtc agcttaaagg aggtaatgaa gggctaaaag
     5461 ttaagtttgc tagcaatgtg gatgaagaag ctaaaaaagc tacagttaaa aacattgctg
     5521 atttttatcg acaaaacggt ggactacttg tggaagaaaa cggtgtagaa atcgaacgtt
     5581 tacaacgaga gctggtagac agcaagcttt tagatactga taaagtatct cgctccagaa
     5641 tcgcgatggt ctacaacgtg ccggaacatt tcattgggag taaccagtcg agttactctt
     5701 cacaggaaca gctcaatatg gagtttttga catacaatct agtaccgacc gttaatcaat
     5761 atgaagcgga actaaataag aaaacactat cgagaaccga aaaagcaaaa ggttatcgat
     5821 ataagtttaa tgtcgcaagt ttgctaagag ctgacactca ggcaaggggg caattctatc
     5881 aaatcatgcg acgaggcgga gcatactcag cgaatgatgt ccgaagattt gaggacttgc
     5941 aaccgataaa taaaaccggt atggatgatt atcatatttc cggagaccta tatccaatcg
     6001 atatggatcc aacattaaga aaaacaaact cgtctaaaag cgtagccgaa aacggttagg
     6061 cttttttagt ttgcaccgaa gggaggtgaa aggatgaaaa aagtgacgtt aagcggcgat
     6121 gtcgtggata acgataccgc gtggttatat gactggtttg ggattgattg tatctcacca
     6181 gggaaaattt ctgccgctct tacagaagca gcgggggatg aagtagaact tgatatctca
     6241 tctaacggtg gggatgtcct agcggcaagc gaaatatata ccgctatccg tgcctatccg
     6301 ggaaaggtat ccggaaatgt tgtgagtatt gcagcaagcg ctgcaagtgt aatcgcttgt
     6361 gcttgcgaac cgcttagaat ctcgccaaca gcacacatca tgattcacaa cgcatgggtg
     6421 accactagcg gcaacgccga ggaattaaaa gccaatgcgg aaatgttaag cagtgtggat
     6481 gagtctatcg ttaatgctta cgagatcaag acaggactcg accggaaaaa gcttgctgat
     6541 ttaatggcaa aagatacttg gttaaacgca caaacagcag tggcggaagg ttttgcggat
     6601 gaaattatgt ttgcagaagc accagtaacg gtactcaatg cctctcaacc ggttattcca
     6661 aaaaacgcgg taactaagtt gaaaaatcta atactcaaag cggaaacacc gcaagaagaa
     6721 acgctcttgc agaaaaaact aaaagcctta aatggaggga aaaacgaatg aatttagaac
     6781 aattaaaaaa tgcgtgggtc gaggcgggaa gtaaagtctc tgatttaaac gcgcaactca
     6841 atgcagcatt ggttgacgat gaaaaaacag aagaagatgt agtaagtttg caagcacaag
     6901 taaaagcagc acgggctaaa cgggacggat tgaaagagca agtggcaaat atggaagccg
     6961 aacaagtctt aaacgtcaaa aaagaaccat tagataaaaa agatgaaaac ttgaaaaaca
     7021 agtttatcaa agactttaaa gcgatggtca atggtgatcc tgctattatg gctactttga
     7081 catctaatac ggatgaatct ggtaatgcta tcggattgac tattcctgta gatgtgcaaa
     7141 cgactattca tactttggtt cgtcagtttg actctttgca agaatacgta aacgttgaaa
     7201 aagtgaccac tactagcggt tctcgggttt atgaaaaatg gtctgatatt aacccactga
     7261 ccgctttgga tactgaagac ggtgaaatcc cagcaaatga tgatcctgca ctttacttga
     7321 tcaaatactt gatcaaacgc tacgcaggta tttctacagt aactaacagc ttgctaaaag
     7381 atactgccga aaacattttg gcatggttgt ctaactggat cgcgaaaaaa gtagttgtta
     7441 ctcgcaatac aaaaatcttg gcagctattg atggaatcaa agcagcgcaa aagaaagatg
     7501 ttacagatgt tgatgggatt aaagatatca taaacgtcca acttgatcca gctatcgaag
     7561 ctacatctat gtttattaca aaccaagatg gcttcaatgt tttagataaa gtgaaacgtg
     7621 ctgatggatc ttacttgtta caaaaagacg tagcttctgc aactggatat accttcttag
     7681 gtaaaccaat caagaaaatt gcttctcgtt tcttgccaaa caaaggtacg caagctactc
     7741 ctaaatatcc actgtatatt ggtgatctga aagaagctgt tacattgtat gatcgcgaaa
     7801 acatgagctt gctgacaacg aatattggcg ggggggcttt tgaaacagac accaccaaag
     7861 tacgggttat cgatcgattt gatgtgcagt tagtcgatga tgaagcagtt gttttagcta
     7921 catttacatc tatcgcaaac gaagtgccag taacaaacgg ttaaggagct gatttcttat
     7981 gattcttgat cctaaaatgg atttagacga aatcaaaaac gcactaaaga ttgataccga
     8041 tgatgacgat gtggaagtaa gacgtgcggt gcaagctgca attgcataca ttagaggggc
     8101 tatcgggaat gataagccct ctttttataa gcaagaaaat gacacggttg atctgattaa
     8161 tttagctatt ctgcaattag cagatcatta ttacaacgct cgatcagcaa ccgtgagtgg
     8221 gaacttgcga gagtacgatt taggttttac aagcctaatc ttgcaactca aagcaagata
     8281 tttgcttttt gtggaggagt agcgtatgcc ccttattcaa acaggaaatt taaatcaacg
     8341 catcaagttt gtccgagata cgacggtcaa ggacgaggac gggcaagttg tccggacttc
     8401 tacaaccatt cttacttgct gggcaagtgt gcagacacaa cgcctgaacg atattaagac
     8461 gtccattggt acggctttgg aaggaacact gacgttcatt atccgctatc aacaaaaatc
     8521 agagctaacc aatgatatga aagtgcgttg gaatggaaaa acgtttgaaa tcattacgat
     8581 tacgaaaggc gagtttgcga aggacttcac gacaatcatt gcaaaagagg tttcaaaatg
     8641 agtgtagaag tcgatgcaac cgaagtgtac aaagcgctta gggaagtaaa agcaaacgtt
     8701 caacgagtgg aaagcccagc acttagaaaa gctggggagt acgctcaaga aaagttacaa
     8761 caaaacacac cttactggga tggaacgaag tcaaacggta aacgtggttc gtatatgcaa
     8821 gaacatgcta aggaccatgt ggttacaagc tcggtaaaaa acggattgat agaagtcggc
     8881 tatgataaag atgtttcttg gcggatgcac tttatcgagt tcggaacaat caaacaacgt
     8941 ccaaaaggtt tcgtacaaaa aacacaaaag caaatcgaaa aacaagtaac acaaatcatt
     9001 gctgacgaag taaaaaggag gctaggactt tgaaaacggc agtatcacaa gtctattcaa
     9061 ttctgaatag caatgaaaaa acaaagaaca ttgattttta caccaatagt gttccggaat
     9121 cggctcaaac agtacctagc cttccagttg gcagaattac agagatattc ggcaactatg
     9181 aagatttcgc aagcaacaat cctttgacca ttcaatttaa cgtacaggta gatgtatggg
     9241 tatcgacctt aaaagaggtt gatgcctttt attttgccct tgatgagatt atgaggggga
     9301 atggttggca atgcgcatac acggaacaaa cagatgatga ggatttagaa ggtgcaaagc
     9361 ggattatcaa acgatatgta gcaaatattt cactaaatta aaaggagaga aaatagatgg
     9421 caacagtagg atttgagagc gtcatttttg gcgtaaaaac aggtgcaggc ggcactctaa
     9481 aagaattagt agcagataag tcgaaaggcg gagcgatcga agctaaaatt actggattag
     9541 gcgcaacttc taacacaaca tacgcttcaa acgtaccatt cttcattgca agtaaagggg
     9601 tttcgtcgcc aaaagttacg cttgacgtgg cagacttaat ggataacggc atttacagcg
     9661 aaatcattgg cgctaaaacc gtggatggtg taaatgtaat tggttcagaa actgaagcgc
     9721 cttacgtgtc ggtagtcatg gttacagcga acaaagaagg aaaacgctta ttcatgggat
     9781 tggcaaaagg aaaattcagt catccagata tcgacatgaa aacagctgaa gacaaaggag
     9841 ttgaattgca aaccgattct atcgaagggg aattcatttc tgatgaacgt ggctatgtat
     9901 acttaacagc cgtagaatca gaagaaatga ccttacaaaa attcaaggac ttggtaaata
     9961 acaaagcggg ggagtagtta accctgcatc tacaccaacg acagatacag ggacaccaaa
    10021 agaaccagaa ccaaaaattg atacacaagg ttagccattt tttggctagc cttatttttt
    10081 tgtaaaaaca aggaggaaaa caaatgattg aattgcaatt gaaacttgac ggaaagaaaa
    10141 aaacattcaa acaacaagat atttccgcac gtgcaatgcg tgagtgtatc aaattttacg
    10201 agaaagcgga aaaagcagac ctaactgatt tagaagcaat tgattcaatg attgcaatta
    10261 cagcagatat tttccaagat ccagcagtta catttgatgc tattttagac ggtttgactg
    10321 cgagcgagtt agtaccggca ttagaaagtg tttttgaaca aatcaatgaa ctgggaaaca
    10381 atgaaaaaaa gcagacggcg agcaaaaaga gataagtttt tctgaagcta ggaaagcaat
    10441 ggatcaaatc tacaaagatt taatcgaatc aggttggacg atgagagatg tggacgaagc
    10501 cgactatcat tatttgttac acctttttgg agaagtggag agtggcgaag aatatgtaga
    10561 tggtgctgat ttcatcaaac aatttttatc ggctgaagac ttggtaaaac ttgaggaagg
    10621 aggtaaataa tggcaggaaa aggacaaccg gcaggaaata tcaagctagg gattagttta
    10681 gatagcacta attttggtaa cacgctggac gaaatcaatg cgaaagtcaa acaagctgag
    10741 tcgaatatgc gtgccaatct aaaggcttat gattcagcag gacgttcata cgaagcactt
    10801 agtcaaaaga cgaaagactt gtctacggtt atggaagggc aaaacgccaa agtaagagaa
    10861 ttaacaaagc gccgtgatga agcgattagc aagtatggcg aggaatcgaa acaagttgct
    10921 aaccttaaca cacagataaa caatgctacc gcaaaatata atgcttacag tcgccagttg
    10981 aatgacacaa aaaaagaatt ggtgtattcc aaaacagccg tcaatgattt atctaacgaa
    11041 atcaaagaaa atgaacgaca aatgaacgcc gaagtaaaag cgttgaaagc cgctggtgat
    11101 gaatctggtg cgtttgaagc aaaacaaaaa gggctagcca aacaaacgga attatccgag
    11161 aaagctatcg aagaacagcg caaagttgtg aaactgatgg ctgatgagtt tggcgattca
    11221 gcaaatgaaa ccgaagatgc aaaaagggca ttagaaaagt tagaacgaca aagccaaata
    11281 tctagcaggc aattagaagc actcaaaagc tccagcgatc aatcaggaaa aaaaatagaa
    11341 gattttggcg acaagtccac aaggtcagct aggaaactgg atgggctaaa agacaaatta
    11401 ggctcgctaa aaagcgcatt ttcgtttggt gcagttgctg gattagcgca taacgctatt
    11461 agcagtgtag taagtggcgt gcaaggcttg gttggcgaag cagtaaacgc atcggattca
    11521 ttgatgaagt tttccaaaac catggagttt gctaactttg ggaagtcaca gatagaaagc
    11581 tcgaaaaaag aaatgaaaga ctacgccgat aagacggttt atggtttaga agaaattctg
    11641 aacacaaccg cacaattggc atctaacggg attcctaact atacagaact aaccaaggcg
    11701 gcaggtaact tgaatgccgt tgcaggcggt tctagtgata cattcaaatc cgttgccatg
    11761 atgctaacgc agacggcagg agctgggaaa ctaacaactg aaaactggaa tcaattagca
    11821 gatgcgatac cgggcgcttc agggctgtta caagacgcta tgttgaaaaa cggagcttat
    11881 acaggaaact tccgtgatgc aatggcgcaa ggtcaaatca cttctgatga gtttaaccaa
    11941 gcgattgtac agttaggtat gaatgacgga gcagttaaag cagctacttc cacagataca
    12001 ttgagtggtt cttgggaaca gatgaaatct actgtaataa atgggttaca aagtatcata
    12061 gacaaagtag gcgttgaaaa tatcactggt ttcatcaaca gagtaacaaa agggattgaa
    12121 aattctattc ctaaaattac tcaatttata ggttggttga gagatattgg aacgtggatc
    12181 gttgaaaata gagagccact aacatggatt gtcggaatca taggcggaat tacattagca
    12241 gtaaaagcat tgaacgtagc aagtatgttg ctggcaatta ctggcggaac attggcagcc
    12301 ccttttgtgg cgattggtgt agcattaggc gcactagcag gtgctttggt gatagcttat
    12361 acaaaatctg aaacatttag aaatatagtc aatgcggctt ttacagctgt gaaaaacgta
    12421 gttatgagcg ttatcaataa tttggtggaa tactacaaaa tgttgtggag cgtgttgcag
    12481 tggctttggg aaaaaataaa agaatgggct tcatggattg gcaataaatt cattgaaatg
    12541 aagaacagcg ttgtgaatac ggttcagaac ctatggaatg gtgtgaaaaa cttcttcagt
    12601 aatggcgttg gagacacttg gaataaggta gttggctggg taaaaaacat tttcaacaaa
    12661 gcaactgaat tgaagaacaa agtttctgat gcaattggta acctgtggaa cggtatcaaa
    12721 gacacattcc gtagaggcat tgatacggta ttcaattggt tttcagaact accgacgaag
    12781 atgaagaatg ccattattgg cggtaaaaat gccattgttg atgcgttcaa aagtattttc
    12841 aacgcagcac ttaaagcgat aggtaaacca gttaacgcaa tcatccatgg agcttcatgg
    12901 gtactagaaa aactgggtgc tgacaaactc gaagaatgga aagtgccaca atacgcaaaa
    12961 ggaacaccaa acggaggtca tccgggcggg cctatgatgg taaatgacgg tagaggtgct
    13021 gaagcggtaa tcacacctaa cggacaagca tttatcccac gagggcgaaa tgtagtgttg
    13081 aatgcaccaa aaggcacaca cgttctaaca gctgaagaaa cagcttatat gactggaaac
    13141 aaagcaccaa gatatagata cgccaaaggt acaggctttt tcggaaatct atggaacaac
    13201 gtcaaaggat ttgctggaga tgttggaaac aagctgaaag atgtagtcgg cgatgtatgg
    13261 gattttgtaa cagacccggg agcgttggct aggaaagtgt taaatggtct tggcgtactg
    13321 gaagggcttg tcaaatatcc tttagatgtt ggtaaaggta ttctaagcaa ggctaccgaa
    13381 gcattgacga acaaaatcac agaactattc agcagtggca gtttagacac ttcaatgggc
    13441 atgcaaggcg tttacaaata cttggcggac gttgcagttg cagtaatgaa gaagtttcca
    13501 ggctttgtgg caactagtgg gtatagacca ggtgacccct attcgcatgg taaacgtaat
    13561 gccattgata ttgcactacc tggtgtcaca ggaggctcgc cacgctacac agaagcagcc
    13621 aattacgcat ttgagaagtt tgcaaacaaa atcggctatg ttatcacaaa tggcaaggtt
    13681 cgtgaccgtt caggacaatc aggtacaggt attcacaatg attggagacc atggcccgat
    13741 ggagatcact atgatcatgt gcatttaaac ggtgtgaaag acccacaaaa cactcaaatt
    13801 tcaggagata gcgtgggagg cagtggggta gaaagatggc gcaatgtagc aattagagcg
    13861 ttgaaaatga ccggtcaata cagtactgca aacttaaatg cattactaaa tcaaatgcgt
    13921 acagagtcaa atggtaatcc taatgcagtt aacaattggg atattaacgc caaaaatgga
    13981 acaccatcaa aagggttgct ccaagtgatt gacccaacat tcagacagta tgcaatgcca
    14041 ggattcaaca gcaatatttt tgacccacta tctaacatct tagcttcaat cagatacgca
    14101 ctatcaagat atggctcact aacaaatgcc tatcgtggag ttggttacgc aaacggtgga
    14161 attgtaaacc aacatcaaat tgcggaaatc gcagaaggaa acaagccaga aattattatt
    14221 cctttagata aggctaaacg atcaagagcg atgcaattat tgtctatcgc aatggacaaa
    14281 ctaggagttt ctcctaaaac ttatggaaat gcgacaactg tttccagtga ttacgaaatc
    14341 ctagaagcca ttgaaaaaca ggcggcaaca acgaatcagc tgttatcgtt attgctggca
    14401 tttttcaaag gaaatagccg aactgataga gatttagctt tagatattca gaagatcttg
    14461 gttaggagga tgtagatgcg aactgtatta ctgagaaata gaataaacga agaaattgat
    14521 ttgtctacgg aggattattt tgctactggg ttgagtaata tggggtttga agtaaaaaaa
    14581 gaacatgtgg gacagtgggg gaatttcaga gaaagcagtg agaacgttga aatatctgaa
    14641 tttcagtcat ctgtaatcat ttctgtgcat gggtttcgag aaaaagaact gtacaattca
    14701 cttgtgcagt tcctatcgga aggtccgttt gaactggaat ttgcttttga cggcgaaaca
    14761 atggtaagaa gatgcagtct aaaatcttta agtaagactg aaatcgatcc gaaaacatcg
    14821 ttactaacag acactttaga actatatttc acatcaaact ggtattccgt aaaacgcgaa
    14881 aaattaattc agcgaccgaa tgtagtgaaa acacgtggta aggtttttcc gtataaaaga
    14941 tcttatatct acactcaaaa cttgtgggag aaaaaaggtg tattcaaatt caataataat
    15001 tctgtatacc taacgaatag caaggaacgg atgtccccgc taaaaattcg tgtgattggg
    15061 aagtgttcaa atccgtattg ggaagtaatc caaaactcac aaatcatcgc aacggatgga
    15121 tacttcatag atatgactga aacacagact ctagaagtat caagcctttt tgaagataca
    15181 acggcgattt taaaagatat tgcaggggta gaatcttctg tctaccaaca acaggattat
    15241 acaaaaacta attttgttca ggccccgact ggagaattca gcattgtgtt tcatgttggg
    15301 ggggcagacg tagagattga gttatacgag gagcgtgatc tgttttgatt ttagcagtca
    15361 cgctttttca tcgtgattta atgctttata acgaacattt attctctaac agttttgaat
    15421 ttggtgcgga cgagatcaac gaagaggcta gtagctttac gatggataag tacgttcctg
    15481 taaaaacagg cgattttcta ctagcaaaat atattcctag tggaaaattt gcatattttg
    15541 gtgtaattac gtcgcaggaa gacgaaaaaa tcagttgtaa aagcttactt agtttagctg
    15601 atagtgagat accgactgca cgtgtgtcag gagataacta cgaagagcat atccggcggt
    15661 tgattgaata ctatttgctg aatgatccaa cgaaacaact aaaagatatt ttagacgtca
    15721 aagcagaaag tgcgacctct cattcgtatc aagctaccga tacgaataaa cacaagttaa
    15781 gtgcgtatat tctcaatggt ttcaaaaaat acaacgtaaa atggtatttc aagggaattc
    15841 aaaacagaaa aatctataca ggcatacgtg ctgtaaatga atcaatttac attaaagaca
    15901 attcttctga atttagcgat tgggatgtgt ttgttcaggc gccgggcgct ggaaacgaga
    15961 acaagctatt aatcgttgat aaagcaatga aagatataga gaaaccgata atactgtcaa
    16021 catggtattt ggacgaagaa aacaatttga cacaagatgg atcgaaagaa aatatcacga
    16081 aaccaactgt caatttggtt aatatctacg atcaaaccgc agaagataaa gcatcctacg
    16141 aagatgtggc aaaatcagag ttgaaaggca atacgtattc gcatgaaatc aaagtgaacg
    16201 ttgtaagaaa tgcaaaaaat ttgaatgtcg aaacgattga aacagggatg tttgccacga
    16261 tttcttataa aggaaagata tacaagtcgg ttttgacagc ttggcgaata tcaagtgata
    16321 aggagtttgt ggaattgact ttcggaaaca taagaagtcg ttttatggat tattttgaag
    16381 ataatggggg ataaaaaatg gttagcaatg tggatggata tcaatttgaa aacgtgaaag
    16441 taagcgcaga aaatgatgct agactttatc acgttttata taatcggaaa aatcaggtta
    16501 ttgatggtta cgatcagtct atgaatttat cttcaagcgg attaacagta aaagttgctg
    16561 caggagcagc gattattcaa ggtcgtatgg tcgttgttcg acaagaagaa agtataacag
    16621 ttccagcaaa ctcaagtggt tatatagcat taacagtgga tttgacacaa gaagttatac
    16681 ctggatctat tcttccagaa tcggaagaat atgaatggac taataatcaa gtcaagctag
    16741 aatttataac aaaagttata aaaggtaatt taaacaatgg tgataaggtc tataatttac
    16801 cactatgctc agttacttct actggatcaa ctgtttcaat ctcaaagata tcggatagtt
    16861 acgagctgac tctctctaaa ggagaaattt tgtggagtgg gactgcgtta atgcatgata
    16921 ctcaaactgt ccaaccttca aaaaaaatgt ggcagacagt ttcaggtttt ttattagtat
    16981 ggctcccata cgaaaacggg caagcaattg gggatagata tgtaactacg cctttttata
    17041 aagagcgtgt aacttttact aatgttttag gagaaattgt ttcaggattt gatgactatc
    17101 acaaaaagtg gtttagtaaa cgaataaact ataattcgaa tactaatata tttacaggtg
    17161 cagcaagcaa tgcaagtgga gataacgcaa atatggtgct taggtatata gtttcttttt
    17221 agttaggagg tatggaagag tggcaaattt agaaattaaa ttatctgcaa ataagagaca
    17281 gccttatcta cgtcaccgtg ttgttggtag agttggtgat ggggggctca caacaatcaa
    17341 tgtacaactt cttgaggaag atgaaattac accttttgta attaatctaa acggtacttt
    17401 gaaatttgtg ggcgaagttt caaacggtaa ctataccgag ggagaaccag aaataatcga
    17461 ttcgactaat gggttaatta gttacacgtt cactaagtca aatttcagca cgagtcatca
    17521 attcaaacaa gcatattttg aatatgtaga tcctaacggc aaaaaagtaa cttttcagaa
    17581 cttcatcata gacgtgttag aacgagtaga tattaattcg gagcaagcga aatactatat
    17641 ttcttcattg gaaaaattac agagtgaaat gcaaaccact ttcaatcagt tcattagtga
    17701 taaacaggtc caatacgatc aaatctactc gaaatataac gaattagtaa gattgataaa
    17761 tgaatcagat aagcaagtaa atgatcgtat tgataagaca aatgatcgta ttgatcaaac
    17821 caatcagcaa atcggcgatc tcggcaagct gaaaaagatg tacagtaaca gcatcgactt
    17881 cggggactat gattatagtg ggaatccgaa ccttagcgct aaacttaatg cttcaagttt
    17941 ttctttaggt actggcgcaa cagtcgcgga tgataatgat gagattgtgt ttaccttaga
    18001 tggcacaaat caactatcga agtacgctag caaagcgcaa gtgccggtag cagaagggaa
    18061 acaatatacg attagttgtg aaattatgtt agaagatggg ttcactggcg acccttcagg
    18121 aatacgtctg caacacgcat atttgccagg cggggttgtt gttttacaga cagagacagt
    18181 acctaaaaat gaattgaata cgtggcagac gcttatcgga acgcgaacgg taaaatacac
    18241 ctctgacgta ccaaatgaat ggtacccttt gtttagagac atccgaacac ttaaaccgac
    18301 cggtaaagtt agactaagaa aaattaagat tgaagaaggc tcaacagcca ccccatatca
    18361 gccaaactta cttgatgacc cttactggct aggtaaagcg cctttgggtg agaatattgc
    18421 taataagtct gttacgtttc caatcaaatc tagcgcctac agcctatacc aagctaacat
    18481 ggaagaagaa tttgttttag gtcaaaccta tacaattacc atgaaggcaa ccaaaccacc
    18541 tatccaaacg ttcattgttt acaacgagga tagtggagat tatagatatg gtaacttaga
    18601 gccagtagaa gggttagttg atacatgggg tctgactttt acaccgcaga aggttggagt
    18661 taactacccc aaacggttaa ctattatcca gtatccccaa tcaacaacag gtgcatgtca
    18721 aattgactgg ctcaagatcg aaaaaggcaa tactcgaacc ccgaatatta gtgagtataa
    18781 atatcgtggt actggtatgc gtgattcaaa caatccaaaa gattatgttt gggatctagc
    18841 accagaatat gtcgaagata atttggccac agatattaaa atttctgaaa ttactggtaa
    18901 agcaaacaat tataccgatg ggaaagtatc ggagattaat tcgcagttga ctgcttcaat
    18961 taatgaagta gacaccacag ctaaggatgc tcaaacaaaa gcgaatgcta atgcgactgc
    19021 tatagatgaa ttagacaata agatcgatga acgcattaat gatacagcta ctaccacatt
    19081 aacagttaca aacgggaata ccggatcagc aaagctttat cgtgaaggaa aaacagtttc
    19141 tatatatttt gtggctttaa acggaaaaag cagtggtgga aatgattcaa cgatactaac
    19201 aattccagaa ggctatcggc caccaattag ttttgagcaa ctggttggct cgatagaccg
    19261 ttctactttg aacagtgctc agttatctat tggtgcagat ggagccatta aatggcgaag
    19321 aaactcaagt tatggatcgg attatacctt tgcaattact tacacgattt agaaaagcgt
    19381 gaatcgatat gaaggcagca tatagaccaa ttgaacctta cggattcgag caaatcattg
    19441 tgaatgatga agaacattta ccggaagaat gcacagaagt cgaaccaccg attctaaatt
    19501 ggaaaccgaa attcaattac tgggagggaa ataaatgaaa aacatttgga aatatggacg
    19561 tactggcgga gagtacgcag gaaaagtatt ggacgacatg cttgtatccg ttccttacac
    19621 agatcagcca ccgcttgaag gaattcgtgc tgatggcgaa ccgttaacaa tcgctgatca
    19681 aatgtttgat cctaaattga atcaatggat tattttagcg aacgcactag atcacaacga
    19741 tttaaacaat ctcaaagcaa tgtatgagtc gttagaaaat gagaacggcg atttaaaaca
    19801 gatcaatgcc aaactcatgc taagcgatgt agcaattaaa caggaaaata ctgcattgaa
    19861 agaaaaagcg gatagtttag cacaaatcaa ttcaaaaatg atgcttgctt cgttacaaaa
    19921 tagcaaagac atttcagaaa ttaaagagca actaaatcca gcttcaaagg gaggtgagta
    19981 gtatgtttag ttttagcgat gtgaaaatga tgtatgattg gggctgtttt actgacgatc
    20041 aagttcgact attcgttcca ctatgcatta cagacgaaga agcagataaa atcattagca
    20101 aagaagaaag cgcatcttaa gtgatgcgtt tttatttaag gtaaaggagt tgtcacatga
    20161 tcaatttagg ggaatgggga acaatcgcag gatcaattac tgcgattgtt tctttgattt
    20221 tattagtaat aagaccagtc actgcatctt tctcgaagat tactgaaact ctttcaaaag
    20281 taagtcacaa tttagatttg ctgactaaag atttagaatc gagcaaatca gatcgattga
    20341 tgattcatga agaactaaag aaacacgatg aaagattaga tacacatgca gaaaaattgg
    20401 tagaacacac acaacaaatt aaaactttat ttagagaaag gtctcggtaa aaaagaaagg
    20461 agttaagaag aaatgatttt acccgataag tattatcaag tcattaaatg gacagtttta
    20521 acagtattgc cagctttatc tgtattagta gccacattag gcaaagcata tggatggaat
    20581 ggaacagata tggcagttct gactatcaat gcagcagcaa catttttagg tgttatcact
    20641 ggtgtgtcgg cttataattt gaaaaaatag gaggaaacaa atgaaaaaga aaattactat
    20701 tactgcgatg agcctattaa cggctctttt tttattgccg attaatgggt ttgcctatac
    20761 gattaacaat gaatttaatt taggtgtaaa cgaaggtagt tctcaagtag caaataatca
    20821 gtacatttta ctgcatgaaa cggctaatga aacagcaaca ggacgcaatg aagcgcagta
    20881 tatgcaacgt tcatggacta gcgcttatac tgcttatatt gtgggagacg gcggaattgt
    20941 ttatcaagtc ggtcaacctg gttatgtaca gtacggtgct ggttcgtatg ctaatgcaaa
    21001 tagtcctgtg cagattgagt tacaacacac acatgataaa gcaacgtttg agaaaaacta
    21061 caaggcatac gttgaattag ctagagattc agcaatgaaa tatggtattc cacttacact
    21121 agacacaccg tataatcaac cagggatcaa atcgcattta tgggtaacac aaaatatttg
    21181 gggcgatcat acagatcctt acggttatct ttctgaaatg ggcgtaagta aagaaaaatt
    21241 agcctatgat ttggctcatg gatttaccga tgaaaatccg acaacttcag atgataaacc
    21301 agtcattgat ccaactcgag caggtgctgc aaatcctacc ctgacagatg gaacaaatta
    21361 cgcccacatt gatcagttcg gagaaatcga aaacgcaaac ttacatgtgg ctggttggca
    21421 catcgctaac tataaatacg agtatatctt cattatggat tacaatactg ggaaagaatt
    21481 agctcgagta aaagctgatg gaatatatag accagatgta aatcaagctt ataatacttt
    21541 aggaaatgtt ggctatcatg tatcttttaa catgcgcaac ttccctaata agaaagtcta
    21601 tgtaatgatg cgggcaacga atgatccaga agggaacact aaaggcggag cacaagattt
    21661 tcatgataaa cgctggtatt taaatattcc gcaacgataa aaatagcccc tcgtttttga
    21721 ggggaagtac ataatgcaaa tttgtccata acttattgat tatcttgtaa aaatgcggta
    21781 atttaatagt tagtgagaag aggtgcttaa ttatagttaa aaaagaggaa atagaagcag
    21841 catgtaacca agcaaatgtt aggtttattg gagaagcttt atctggatat cctaagtata
    21901 aatacttaca caactggtta caaagtaaaa gtttttatct taataaagaa agatgcatgc
    21961 ctaataaaaa ttatagggtt tataaaagag gaactattgt atatgttgat tttggggtga
    22021 acgtgggtta tgagttatct ggtaatcatt ttgctattgt tttaaataat aaagataatt
    22081 cgaaaaatgg tgttgtatgt gtagtaccaa tctcttcaaa aaagaaaagt aattatgtat
    22141 ctgtaggaaa aatattagaa gttgctagta ttaaacaatt tataagccaa gctgataaat
    22201 taaaagagaa acttaggata attattttac tttcattaaa tagaggcatc attgatgaaa
    22261 gcgatttgaa tagttttatt aaagatattg aagctaaagg tgaaaagctt tcagtggaag
    22321 aagtagaaaa acgagctgta aaacataatt tggtttgcga gacaagggaa caagtaatta
    22381 aagaaataaa atcattatct gaagatatgg taaaatatca gaaggtgttt aattgctaca
    22441 agaaatatac acaagaatcc tatgtaatgc cacttaatat ccaaacgatt agtaaaaata
    22501 gaattagaag aattaacaag tttgatcctt ctggaaatat gaaggcacct ggaaatgtcc
    22561 tcaatgaagt agatgaaagt ttaaaaaaat attttacaaa gtaaacgata tttttgttga
    22621 catttttctg ttcgttagtt accatgtagt tatagaagga cgtaagcgcc tatttttaat
    22681 gtacacatcg ggacgtatgc gcccacatag aaacatagtt gggctctttg tcaaatagga
    22741 ctgatagagt gagataagaa catctaaggt agaattcctt ttctacttta gatgtttttt
    22801 gattcatagg taatcagatt atttagtaag aaaattctgg tcttcatttt ccgaaagctt
    22861 cgaaagccat aagcgactcg tttcaatgct ttgatgtggg tatgcagatt ttctattttg
    22921 gcatttgaat aaggcaattc gagggctaaa cgaatgccgg attcatagtt tataaaagct
    22981 ttttttatag aatgcagttc ttttggaacg gaattgggca tcgttcttac aagtttaaag
    23041 aaagtgtccg cctctttgtc gtgaaacgct tgaagaattt cttgatagta aaaataggcg
    23101 attcgtaatt cgtcagagat cgaaagcaga cgatccacca cttcactttc ggtgagatac
    23161 gcccaaccaa aattgcgcca tttggtccat ttgtcataaa caagcttgtc ctgtcgtttg
    23221 atcagaagcc gagacaacgc ctttaattgt cgataatatt tggcttgtgt tggatcgttt
    23281 cgatcaaagc ttttcatgat tcgtacgcga atatcctcaa atttacgaat gagatgtttg
    23341 acaatgtgaa aacgatccac aacgagtttt gcctttggaa aacattcctt tatcagagag
    23401 gcataagaaa cattcatgtc cgtcacgatg acctttacct tcaatcgtgc gcgtcgttca
    23461 aattgcataa aataagaaac gagagccttt ttcttccgtg aaggaagaat atccagtaat
    23521 tttccagtat cgccatccat acaagaaaag gagtagggac cgacttgatt tttcagtgaa
    23581 cgaaattcat ccattaataa acaacgcggt aaccaatttc tttttacttt tgtcatgggg
    23641 atcagagaag cgagtgtccg agaaacggtc gaccaagaaa cgccgcaatg cttagcaatt
    23701 gtcgtcattg cttgtttttc agttagctct tctaaaattt tatagcgcac ttgtttagcg
    23761 ataaagcagt ttctatcgac aatgtaggta ttcgccgtaa aataggtgtg gcagccacga
    23821 cagtggtagc gttgtttcgc taaagcaaga taagtcagtt ggttcgcaca tttattaagt
    23881 tgaatgagcg attttttctt tccgttctta acgatcatgc cttcatgatt catctgttta
    23941 cacaaaggac agcgacgaat ttctttactt aacgtcgcag aaacaacaag actcttgata
    24001 ttcttaatgc tcttcgattc cacatttgtg atgaaaatgt ttttatcttt gattccaagt
    24061 agtagtttga tatgatgaag atcaggcata cagaacgctc ctttgattgt gttgtggtga
    24121 ttcaattcta acggagcttc tgtatgtttt gttttttcat gctcaaaagg gaccgataga
    24181 atgattttct atcagtccta gaaattatag agccgatttt gaccgacagg tataaccctg
    24241 ccggtctttt tttgctgcta caggaaaact acagactggc tacagggaaa gtacagatgc
    24301 gcttgccata ataacaatcg taccagccac aaatcgtagt tttattgcaa aggaggcatt
    24361 cgatcaaatg gaaaaaagca actatcattc caatgcggat catcacaaac ggcatatgaa
    24421 acaatccgtg gaaaagcggg cttttctatg tgcgttcatt atttcgttca cagtctgcac
    24481 gctgtttttg gggtggagac tggcttccgt attggaggca acacagatac cgcccatccc
    24541 tgcaactcat acaggcagca gcactgacgt agtggagaat ttggaggaaa acgctcttgc
    24601 caccgccaaa gaacagggag atgaacagga atggagcctg attttagtga acaggcagaa
    24661 ccccatcccc gcacagtacg atgtggagct tgagcaacta tcaaatggtg agcggataga
    24721 tattcggatt tctccctatc ttcaagattt gtttgatgcc gcaagaactg atggagttta
    24781 cccgattgtc gcatccggat accgaacaac agaaaaacag caagaaatta tggatgaaaa
    24841 aattgccgaa tataaggcga aaggctacac ctctgcacag gctaaagcgg aagcagaaac
    24901 ttgggtggcc gtgccgggaa cgagcgagca tcagcttggt cttgctgtgg atatcaatgc
    24961 ggacggaatt cattcaacag gcaacgaggt ttatagatgg ctggatgaaa acagctatcg
    25021 ttttggtttt attcgtcgct acccaccaga caagacagag ataaccggtg tgagcaacga
    25081 gccgtggcat taccgatatg ttggcatcga agctgccaca gagatgtaca accaaggggt
    25141 ttgccttgag gaatatttaa aaccagaaaa atgagaaaag gatgttatgt tatggacaga
    25201 aaaagattga cacagcgttt cccgttcctg cttccaatga gacgggcgca gagaaaaatg
    25261 tgcttttatg cgggaatgag atttgacggc tgtcgctatg cgcagacgat aggggaaaaa
    25321 tcgctttccc atttgctctt tgaaacggat tgcgcattat acaaccacaa taccggattt
    25381 gacatgatat accaggaaaa caaggtgttc aacttaaagc tggcagcaaa gaccttaaac
    25441 ggcttattga taagaccggg ggaaaccttt tctttctggt tgttcctgca tacgccattg
    25501 acaattatcc agcgcagagg tcacgaagta aaggagtttc cagagccaaa cagtgacgag
    25561 attaaagggg tggatgcaac catttcagag ggctggattg atttaaaagt gcgaaacgat
    25621 accgactgca cctaccaaat atgggtgacc ttagatgatg agaaaatcat cggtcaggtg
    25681 ttcgccgaca aagagcccca agcattatac aaaattacaa atggcagtat ccagtatgtc
    25741 cgtgaaagtg gcgggattta tgaatatgcc caggttaaac ggatgcaagt tgccttaggt
    25801 accggggaaa taatagattg caagctgctt tatacaaaca aatgcaaaat ctgttatccc
    25861 ctcccggaaa gtgtggatat tcaggaggag aaccaatgag aaaaagtgtg ggcattactg
    25921 tttttggatg cgaacaggat gaggcaaatg ctttccgcgc tttatcgccg gattttcata
    25981 ttatccctac gctgattagc gatgcgatat cggcagacaa cgcaaaattg gccgctggca
    26041 atcaatgcgt tagcgtaagc cataagtccg aggtttccga ggcgacaatt cttgcgctga
    26101 gaaaggtcgg ggtaaaatac atttctaccc gcagcatcgg ctgcgatcac attgatacga
    26161 ctgccgccga gagaatggga atctcggttg gcacggttgc gtattcgccg gacagcgttg
    26221 cggattatgc tttgatgctg atgctgatgg ccatacgggg tgcaaaaccc accatgcacg
    26281 ccgtggcgca acaagatttc agattggatc gtatccgggg gaaagaactg ggggatatga
    26341 ctgtgggagt tattggaacc ggccatatcg ggcaagcggt cgtcaaaagg ctgcggggat
    26401 ttggatgcca tgtgctggcc tatgataaca gccgaaaaat ggatgcagat tatgtccagc
    26461 ttgatgagct tctaaaaaac agcgatattg ttacgctcca tgtgccgctt tgtgcggata
    26521 cccgccatct gatcggtcag aagcaaattg gagagatgaa gcaaggcgca tttttaatca
    26581 acaccgggcg cggagcgctt gtcgataccg gggcgctggt ggaggcgttg gaaagcggaa
    26641 agctgggtgg tgcggcactg gatgtgttgg agggcgagga tcagtttgtt tataccgatt
    26701 gctcgcagaa agtgcttgac catccctttt tgtcgcagct tttaaggatg ccgaatgtga
    26761 tcatcacacc ccatacggcg tactacactg agcgtgtgct gcaggatact acagaaaaaa
    26821 caatcaggaa ttgtctcaat tttgaaagga gtttacagca tgaatagaat aaaagtcgca
    26881 atcatcttcg gcggttgctc ggaggaacat gatgtgtcgg taaaagccgc aatagaaatt
    26941 gctgcgaaca ttgatacgga aaaattcgat ccgcactaca tcggaattac aaaaaacggt
    27001 gtatggaagc tatgcaagaa gccatgtacg gaatgggaag ccgacagtct ccccgccata
    27061 ctctccccgg ataggaaaac gcatgggctg cttgtcatga aagaaagcga atacgaaaca
    27121 cggcgtattg atgtggcttt cccggttttg catggcaaat gcggggagga tggtgcgata
    27181 caggggctgt ttgtattgtc tggtatcccc ttatgtgggc tgtgatattc aaagctccgc
    27241 agcttgcatg gacaaatcac tggcctacat tcttacaaaa aatgcgggca tcgccgttcc
    27301 cgaatttcaa atgattgata aaggtgacaa gccggaggcg ggtgcgctta cctaccctgt
    27361 ctttgtgaag ccggcacggt caggttcgtc ctttggcgta accaaagtaa acggtacgga
    27421 agaacttaac gctgcgatag aagcggcagg acaatatgat ggaaaaatct taattgagca
    27481 agcgatttcg ggctgtgagg tcgggtgtgc ggtcatgggg aacgaggatg atttgattgt
    27541 cggcgaagtg gatcaaatcc ggctgagcca cggtatcttc cgcatccatc aggaaaacga
    27601 gccggaaaaa ggctcagaaa atgcgatgat tacagttccc gcagacattc cggtcgagga
    27661 acgaaatcgg gtgcaggaaa cggcaaagaa agtatatcgg gtgcttggat gcagagggct
    27721 tgcccgtgtt gatctttttt tgcaggagga tggcggcatc gttctaaatg aggtcaatac
    27781 cctgcccggt tttacatcgt acagccgcta cccacgtatg atggccgccg caggaatcac
    27841 gcttcctgca ctgattgaca gcctgattac attggcgtta aagaggtgat aagtatggaa
    27901 aatggttttt tgtttttaga tgaaatgttg catggcgtcc gttgggatgc caagtacgcc
    27961 acatgggata acttcacagg aaaaccggtg gatgggtatg aggtgaatcg catcatcggc
    28021 acaaaggccg tggcgtttgc tctgcgcgag gcacaaatcc atgcggctgc gcttggctat
    28081 ggcttgcttt tatgggatgg atatcggcca agaactgcgg tggactgctt cctgcgttgg
    28141 gcagcgcaac cggaggacaa tctcacaaaa gaaaaatttt accccaatat agagcgagcc
    28201 gagttgatta caaagggtta tgtggcctca caatccagcc atagccgtgg aagcgcaatt
    28261 gatcttacgc tctaccacct ggatacaggg gaacttgttt caatgggaag taacttcgat
    28321 tttatggacg aacggtcgca tcatacagca aaagggatag gggatgcaga ggcacaaaat
    28381 cgaagatgct tgcgtaaaat catggaaagc agcggatttc agtcttatcg ctttgaatgg
    28441 tggcactata agttgattga tgagccatac cccgatacct attttaattt tgccgtttca
    28501 taatgaaagt attggttttg tatcagattt tggatttgat aaaatctatg ctggtcatgg
    28561 acaatctttt actggaaagt ggttaaagca tcttttgtaa tacattgcaa attatagtat
    28621 tcaatctgcc cagcggcaga aaagaaaaaa accgctgttg ggcagacctt tcccacgccc
    28681 attttgtatt ttgctggcgg cggttttgag aaatcaaggc cgccgttctt ttctggcagc
    28741 aaaaggtgtg gcggcccata ggaggatgcc gctatgctgt tttgctattg ccatgctcta
    28801 atccacttcc accactcaaa aaaagccttg cccctc
//
LOCUS       DAZBSA010000028        28833 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000028 DAZBSA010000000
VERSION     DAZBSA010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.028"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(7..978)
                     /locus_tag="ACHGMT_001112"
     CDS             complement(7..978)
                     /locus_tag="ACHGMT_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFN6834872.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            1684..3063
                     /gene="brnQ"
                     /locus_tag="ACHGMT_001113"
     CDS             1684..3063
                     /gene="brnQ"
                     /locus_tag="ACHGMT_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFN6834873.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
     gene            complement(3217..3663)
                     /locus_tag="ACHGMT_001114"
     CDS             complement(3217..3663)
                     /locus_tag="ACHGMT_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFN6834874.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(3690..3866)
                     /gene="rpsU"
                     /locus_tag="ACHGMT_001115"
     CDS             complement(3690..3866)
                     /gene="rpsU"
                     /locus_tag="ACHGMT_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFN6834875.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(4028..4456)
                     /locus_tag="ACHGMT_001116"
     CDS             complement(4028..4456)
                     /locus_tag="ACHGMT_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFN6834876.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            4548..5408
                     /locus_tag="ACHGMT_001117"
     CDS             4548..5408
                     /locus_tag="ACHGMT_001117"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFN6834877.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            complement(5500..5850)
                     /locus_tag="ACHGMT_001118"
     CDS             complement(5500..5850)
                     /locus_tag="ACHGMT_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFN6834878.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(5865..7592)
                     /locus_tag="ACHGMT_001119"
     CDS             complement(5865..7592)
                     /locus_tag="ACHGMT_001119"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFN6834879.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            complement(7806..8732)
                     /gene="trxB"
                     /locus_tag="ACHGMT_001120"
     CDS             complement(7806..8732)
                     /gene="trxB"
                     /locus_tag="ACHGMT_001120"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFN6834880.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(8877..10310)
                     /locus_tag="ACHGMT_001121"
     CDS             complement(8877..10310)
                     /locus_tag="ACHGMT_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFN6834881.1"
                     /translation="MGFRKDFLWGGATAANQCEGAYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSNN"
     gene            complement(10310..11713)
                     /locus_tag="ACHGMT_001122"
     CDS             complement(10310..11713)
                     /locus_tag="ACHGMT_001122"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFN6834882.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLSADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            11944..12846
                     /locus_tag="ACHGMT_001123"
     CDS             11944..12846
                     /locus_tag="ACHGMT_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN6834883.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKYLLLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNDVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            complement(12886..14766)
                     /locus_tag="ACHGMT_001124"
     CDS             complement(12886..14766)
                     /locus_tag="ACHGMT_001124"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFN6834884.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEAEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVSFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTEPAIYGITLPKK
                     KPFIYSMIGGAVSGIYLMASGTISYIMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDAIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVRQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(14862..15713)
                     /gene="licT"
                     /locus_tag="ACHGMT_001125"
     CDS             complement(14862..15713)
                     /gene="licT"
                     /locus_tag="ACHGMT_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFN6834885.1"
                     /translation="MLIEKVLNNNVVITRDERDREMIVMGKGLAFKKKVGDPILDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVHLNDSVYISLTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GAKSEDGEVSEELFQVIQKKYKDTFGCVEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            complement(16039..17823)
                     /locus_tag="ACHGMT_001126"
     CDS             complement(16039..17823)
                     /locus_tag="ACHGMT_001126"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6834886.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRTTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVLVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            complement(17823..19574)
                     /locus_tag="ACHGMT_001127"
     CDS             complement(17823..19574)
                     /locus_tag="ACHGMT_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFN6834887.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSVKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTK
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIVMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPVKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            complement(19715..19939)
                     /locus_tag="ACHGMT_001128"
     CDS             complement(19715..19939)
                     /locus_tag="ACHGMT_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFN6834888.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            20190..21776
                     /locus_tag="ACHGMT_001129"
     CDS             20190..21776
                     /locus_tag="ACHGMT_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFN6834889.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETVEEIE"
     gene            complement(21842..23617)
                     /locus_tag="ACHGMT_001130"
     CDS             complement(21842..23617)
                     /locus_tag="ACHGMT_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFN6834890.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(23830..24732)
                     /locus_tag="ACHGMT_001131"
     CDS             complement(23830..24732)
                     /locus_tag="ACHGMT_001131"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFN6834891.1"
                     /translation="MLLGSHVSMSGKKMLLGAAAEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            25011..27356
                     /locus_tag="ACHGMT_001132"
     CDS             25011..27356
                     /locus_tag="ACHGMT_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFN6834892.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            complement(27693..28694)
                     /gene="ccpA"
                     /locus_tag="ACHGMT_001133"
     CDS             complement(27693..28694)
                     /gene="ccpA"
                     /locus_tag="ACHGMT_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFN6834893.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
BASE COUNT         8349 a         6099 c         5133 g         9252 t
ORIGIN      
        1 caaagcttat tcttgatgga gatctgcctt ttcataagct ttgatgattt cggcaactac
       61 agggtgccga acaacgtcac ttgcttcaaa attaacaaaa tcgattttct tgatttgctt
      121 taatgttcgt tctgcatgaa caagaccact tgtcgtccct cgaggcaagt ctatctgact
      181 tgtatcccca ttgacaatca tttttgattg ataaccgaga cgggtcaaga acattttcat
      241 ctgagcgact gtcgtgtttt gtgcttcgtc caagataacg aatgcatcat ccaatgttcg
      301 cccccgcatg taagctaaag gtgcgatttc gattacgcct cgttccatca atcggttcgt
      361 atgatccaat ccaaaaattt ggtataaagc atcataaaca ggccgcagat aaggatccac
      421 tttttctttt agatccccgg gaagaaagcc tagattttca cctgcttcca cagcaggccg
      481 cgtcaaaatg attttttgca cttcaccttt tttcaaggca gcgattgcta atactactgc
      541 aagaaaagtt tttcctgtac cggctggtcc tacgccaaat acgatatcat gtttggctac
      601 cgattcgaca tatttctttt ggccgctgtt ttttacccgt ataggttttc cattacggtc
      661 tttgacgatc tcttcctcat acatctcaac aaaataatct aatgtacctt tttgccccat
      721 tttcaatgcc gtcacaacat cgggcgtact tacgtggatt cctcgtttga tcaattcctg
      781 taatgtccga atgactgagg cagccaagga aatctgggat tgctcaccaa tgatctgaat
      841 gatttctcca cgtgtatgga tggttgtttc cgttgtttcc tcaatcagtt ttatatgctt
      901 gtcgtgtgca cctaaaagca tgctgacatc atcagcagcc gtcaatctaa tctctaaaga
      961 actagactct tccgtcaaaa atagacatcc cctttttttc gattcattct ttcctatttt
     1021 accatactca gaagaaacta tatagcaaat aagaaaaggt tctaaaagag ttgcttaacc
     1081 ataagtacca agaaacgtta aaaaactagc cgcttccatt ctctaaaggt ctcgtttgaa
     1141 taaaaaaagc tgatcatgta agctatgcat aagatagagg acaagtattc cgtctaactt
     1201 agcaagtatt ctctgggtat ttttcttgtt tcaaaaaaag aaaacaaaat atttgaaata
     1261 ttcttgcgtt tcaaaaaaaa caggtgtatt ataacgtcaa caaataaata tacagcaaag
     1321 cgtcgaggaa aacagtaggc tattcagtga tttattccca gtgagtcatg taagtgagaa
     1381 atgacaataa atcgaataga cgaacatcat ttccgagtca atgtcgaaaa cgtagatcat
     1441 tgtggctgcc tccattatca ggctagcgga taactttatc gaaaagcgtc cgtgaatgaa
     1501 gaagcttttt taagcgaaaa aaggtggtac cgttctagaa tctagagccc ttttcccaag
     1561 tggaaaaggg ctttttttat attttaaggt ggtggttttc atggaatctg atattagtgg
     1621 tatttctagt ttctgcaacg tttattttgt tactcaaaat gataattatt ggaggaaaaa
     1681 tttatggaag aaacaaaaac ttattcatgg aagcaattat tgatggtcag ttcacttatc
     1741 ttcgggatgt tcttcgggtc aggtaatctg atctttcctg tacacttagg tcaaatggct
     1801 ggatcaaact ggatcagcgc tggtgtagga tttgccatct caggcgctct tttcccatta
     1861 ttagctatct tagctgtagt agtgacaaaa agtgatggtc tgtatgactt agctaaacct
     1921 gtcggaaaat ggtatgctgc cttgttcttg atccttgttc atttaacgat cggaccattc
     1981 tttggtacac cgcggacagc agcaactgct tttgaaatgg ctgctaaacc ctttttgcct
     2041 gaaaaatatg attcattagg tatgttttta ttctctgctc ttttcttttt acttgcttat
     2101 ttccttactg tacatccaac acgtttggtc aaatatgtcg gaaagtattt aaactccttg
     2161 tttcttgcat tattagctat catttttgtc gtttctttat tccaacccat ggggaatcta
     2221 aaccaatctg ctgcagattc ttatcagacg aacgctttga caaatggcgt tttagaagga
     2281 tacaacacgg ttgatgcagt agctttactt gctttaagtg tcacttttgt ccatgcagtt
     2341 cgtgatcttg gttttaaaga taaaaaagtc acagaattaa cagcaaaagc tggtagcctg
     2401 agcgttgcgt tagaagtcat gatttatttt ggtctggtcc ttctcggtgc gatgagtttg
     2461 aataagatgt ctttatcaga aaatggcgga actgcacttt ctcagatcac taatttttat
     2521 ttaggtaact tcggtgctag cttcttaggg gtaatggtca cattaggtgt cttcactact
     2581 gctatgggac tcgttgtttc ttttgcacaa gatttccata aactgttccc taaagtcagt
     2641 tatatgacat ggctgcggtt aacgactttt gtctcttttg tcgttgcgaa tgccggattg
     2701 gataatatca tccaatggtc tttaccagta ttgatgcttt tgtatccatt gtcacttgcg
     2761 ttaatcttac tttccttaac agcaaaattc ttccaaaaga gtccatttgt ctatcaagta
     2821 acgatgttct tttctgccgt tccagccgta ttggatatgt tggcaaattc gcctgcatta
     2881 gtgagtcagc aaactgtcgt agcaagctta ctaggatttt atcatcatta tgttcctttt
     2941 gctgcattag gtcttggctg ggtcgtccca acgttgcttg gttacgctgg cagcttgctc
     3001 ttttattatg tatatcgcgt atcaggatat aaacaagaag caagtgagtt accagaagaa
     3061 taaccaaatc aaacacaatt taacataaga gatattaaac aggaaaaaaa cagctagaag
     3121 aaagaataag attctttctt ctagctgttt tttagttctt tttttacgaa cacaaaaaac
     3181 tgcggataat cactgtccgc agtttaaatg ataaaattaa gattgtaata gttctttcac
     3241 aatcgcattc acttggtttc catctgcttt tcctttgact ttaggcataa ttgcacccat
     3301 aactttgcca aattctttca ttgatgaagc accagtttga gtgattgctt cttgaacaag
     3361 ttgtcgaatt tcttcgtcgg aaagttgttc cggcatatat ttttcgacta ttacaatttc
     3421 actttttact ttttcagcta aatcgtcacg tcctgcttct tcaaactcat gcaaagaatc
     3481 acgtcgctgt ttcatttcac gagacaacac cgttagttct tcttcttctg tcaagtcgtg
     3541 accttcttta atctgctcgt tttgtataga tgacttgatc attcggatga cttgtaaaga
     3601 ttccttatct ttcgctttca tcgctgcttt catgtcatcg tttaatctac ttagtagtga
     3661 cacactacca actccaattc atcacagaac tagaattttt tgcgttttct agctgcttca
     3721 gattttttct tgcgtcttac acttggtttt tcgtagaatt cacgtttacg agattcttgt
     3781 aaagtacccg cttttgacac ggaacgtttg aagcgacgaa gagcatcgtc aagtgattcg
     3841 tttttgcgaa cgactgtttt tgacatgtta attccctccc tccgagctta aaaagtaacc
     3901 gtaattgtta ttgaaatata tctcaaaaaa caagactgtc ctgtaaatat tatatctaat
     3961 gctgttttga ccgtcaagga aatcttgctt ttttttaaaa ttatcaaatg ttattttatt
     4021 taaatcctta ttgacaattt tcacatcttc cgaaaatctc gaatctatgc ccttcgatcg
     4081 tgcatccacc tagctgttct tcgaaataat tcatcggaca catatggatt tctttcgttg
     4141 ttccgcacac tgtacagata aaatgatggt gatggccgat tttcccctga cagcaatgaa
     4201 aacgaaactt catttctcca tttaaatcgg tttcttccag taaatcgatc tctgcaaaat
     4261 catggagatt ccggtaaatc gtatcataac ttaccccttt gaattcctga ttcatatagt
     4321 caaaaacttc tttagcagat acgtagcggt tacgcttcac taaataagtt aacaatgctt
     4381 ctcttttttt agtatattta aaaccgttat ttttcaatgt ttctaatgat tgttcgatca
     4441 atgtctgttt tttcatgact tattcctcca aatcaaaatg attacgaagt acgcattcta
     4501 gagtatggta taataaacat aaaaaaaatg caagtgaggt tttatccatg tcttccgatt
     4561 ctacagaaat cgttactttt tttgtcatct ccgactctgc tggagaaact gcgacaaaac
     4621 tagcgcaagc tactatggct cagtatccta ccgttgagtt caacctattc cgcagaacgt
     4681 tcgtgacaga tgaaaaaaca ttaaggcagg cattgcaaga tgcattagaa gaaaaagcac
     4741 ttgtcctgca tacattaatc aatcaagaac tgatcgagat aacacgttct ttttgtgaag
     4801 aacatcaatt atttagtttt gatgtgatga cgccaccgat atcagaaatt gaacgtttaa
     4861 ctggtatcaa gccaattcgg caacctggtg ccctccattt attgaatgag aactatttca
     4921 aacggatcaa agcaatggaa tttgctgtaa aatacgatga tgggaaagat cctcgtggtt
     4981 ttttagaagc agatgtcgtt ttgctaggcg tatcccgaac ttccaaaacg cctttgagct
     5041 tgtttttagc taacaaaaat ttaaaagttg ccaatttacc tttgatccca caagcacata
     5101 ttcccaaaca gctttgggag atcgatccta aaaaaatcgt gggactaacg aataacccag
     5161 atattttaaa taatatccgt aaagaacgga tgcgctctta cggattaaac cctgatactg
     5221 cttattccga tatcgaaaaa attagagctg aacttgattt tgcgaatgat ctttatgaaa
     5281 aactaggatg tgtagtgatc aatgtggcat ctttatctat tgaagaaacg gcttcgctta
     5341 ttttgaatgc tttagaccta gaagatcata gttattatgg aactgaaaca acagaaaaaa
     5401 gcgattaatc atttttgtct ttttcttaaa aaacactgga gtgtgacaac gaagaaattt
     5461 cgttgtcaca ctccagtgtt tatctatttg cacctatcgt tattgatgtt ccacatgttt
     5521 gagtgtctgg ttcaaaatat ccagtacatg agaatcatca agactataca aaatcgcttt
     5581 tccctcgcga tgagacttga cgacatgatt ttctcttaat aaacgtagtt gatgcgatac
     5641 agcagactgc tccatttcca ctgcttgggt gattgctgtc acattttgtt ccccgtcttt
     5701 cagatatagc agaattttaa gacgagtggg gtcactcaac attttgaata attgactgac
     5761 tttctctact tttgattttt gcgtgtctgg caatccattc acctccaact tgaaaaaaga
     5821 ctgacttcta aagtttacca ctttttccat cagtctcaag ttttttattc ttctgtcatc
     5881 tttttgataa tcgcttcata agaggctact ttttcggctg cgtcttctgt agagcttcct
     5941 tttacaccaa tataaaactt gattttcggc tcagttcctg aagggcggat agcgatccag
     6001 ccattttctt ccaatgtata cttcaatacg tcggatggcg gtgttgttag tttttctacg
     6061 tttccttcac ggtctgttct cgtcaattct ttaaagtctt ctgtttgaat aacttttgct
     6121 ccggcaaatt ctgtcggtgc ttctttacgg aatttttcca tcaaagcaga gattttcgca
     6181 gaaccttctt gtccgctcat tgtcacagaa atcgtttttt ctgcgaaata gccataggtt
     6241 tcaaaaatcg tttgcaagcc gtcatacaac gttttgcctt gttttttata gtaagctgct
     6301 acttctgcta ataaaagcaa tgcttggatc gcgtctttat cgcgaacaaa aggtttaact
     6361 aaataaccat aactttcttc gaatccaaac ataaaagtat gtgaatggtc ttcttcaaat
     6421 tgttggattt tttcagcaat gaatttaaac cctgtcaaca cattgaacat agtagcaccg
     6481 tagtgttccg caattgctgt aggcaattcg ctggaaacga ttgatttcaa taccgctgcg
     6541 ttttctggta aagtgcctct tgattgatgt gcttccaaaa tatattgagc aaacaatgct
     6601 ccgatttggt tcccagtcaa tacttcataa gaaccatctg gcaaacgaac cgctgcgcct
     6661 aaacggtctg catcaggatc tgttgcaatc agcaagtcag ctccctcttt ttctccaagt
     6721 cgtatagcat attcaaaagc tgaatgttct tctggatttg gcgatttgac cgtagtgaaa
     6781 tctggatctg ccacagcttg ttcgggaact agtacgaatt gttcgaatcc agcttgtttc
     6841 aacgcacgtt cgcccaacat tttccctgtt ccatgaagtg gcgtgtacac aagtttcaaa
     6901 tctttcccca ttgtttcaac caattcacga tcaatcgtca ctgctttgat ttcttctaga
     6961 taagcagcgt ccacttcttc tccgatgatc gtgatcaacc cgctatgttt agcttcttcg
     7021 tcagataata cttcgacttt tagcggattg ctgacttcac ggacatattt cgtcaatgca
     7081 tctgcgtctg caggaggcat ttgtccgcca tcttctccgt agactttata tccattgtaa
     7141 gccgctgggt tgtgagatgc ggttaccatg attcccgcaa acgtcttcaa atgacgaact
     7201 gcaaaagaaa gctctggtgt tggacgcaga ctttcgaata cataagacgg gatattgtga
     7261 tgtgccagtg ttttggctgc ttccattgca aattctggtg actgatggcg tgagtcataa
     7321 gcgatagcga cacctcggcg ttttgtttct tcaccttgtg tatccatgaa acgtgctagt
     7381 ccttctgttg cttgtcttac cgtatagatg ttcattcgat tgatcccagg tcctaagatt
     7441 ccccgcatgc ctgcagtacc gaattccaac ggtgcataaa aagcatcttc aagtttttca
     7501 ggatcttcac ccagatcatt taattgtttt tttaagtttt cttctaaatt ttcttcatgt
     7561 acccattgtt catacacttg ttcccaagac atgaaaataa cacctctttc tgaaatatgg
     7621 ttcttaataa gtatagcatt cacttattag atatgaaagt atgaggacca aaaaacgctt
     7681 aaatcaagaa aggattcttt tatcaagcat gattgcaagt attcacataa atcggattca
     7741 gtggatttca cgcgaaaaaa aagagtaaaa gaacaattga aaccgttctt tcactctttt
     7801 taaacttatg ctgttgtttc ttctaaagac tccaagtatt tatacacttg ttgtccagca
     7861 ataccgccgt caccaacagc tgtagtgatt tgtcgcaaat ctttttcacg aacatctccg
     7921 atggcaaaaa cgcctggcat attcgttttc atttcgttgt ctgttggaat ccagccagct
     7981 tcgttcgtca aacctgcttc tctaaaaggt gcagttaatg gatccagtcc aacatagatg
     8041 aatactccgt ctgctgctaa atcaaattca ttgccggttt tgacattacg tgctttcaca
     8101 cctgtcacta ccatttcgtt tccaacgatt tcgtcaacga ctgtatccca cataaaggaa
     8161 attttttcat ttgcaaatgc acggtcttgg atgatctttt gtgcacgtaa ttcatctcta
     8221 cggtggacga tcactacttc agaagcaaat tgtgtcaaat agatggcttc ttctactgct
     8281 gagtccccac ccccgatgac tactagacgt ttgttacgga aaaacgcacc atcacagact
     8341 gcacagtaag aaacgcctcg tccagcaaat tcttcctcgc caggtacgcc taatttacgg
     8401 tggatacagc ctgttgcgat aatgactgtt tttccttcgt aacttttatc ttcacagatg
     8461 acttctttat ggctgccgtg atccttgata tcttgaacga ttccataagc attttctgtc
     8521 ccaaatcttg agacactttc atacatctta taagctagat cggggcccat gatcgaatcg
     8581 aatccaggat aattttcgat ttctgccgta ttgttcattt ggccgccagg ggcaccttgt
     8641 tcgatcatca atacggataa gtttgaacgt gaagcataca aagcagcagt catcccagca
     8701 ggacctgcgc caataacgat tacatcatac atatgtagat tctcctttac ttaatcaagt
     8761 aagcatactt tacagccaat gcgaaaaaaa gtctattgtt ctgcttgcgt ttctcgaata
     8821 gctttattat actcgaaaaa tccgctgaaa aacaatagac ttttataaat cattgatcaa
     8881 ttgttcgaaa gattttctcc atttgaatca attacttttt tataccagtt gaatgatttt
     8941 ttctttgaac gggctaatgt gccatttcct tggttatcac gatcgacata aatgaatccg
     9001 taccttttct tcatctcacc ggtaccagct gatactaaat cgatacatcc ccaagtcgta
     9061 tagcctaata gatcgactcc gtctagttct actgcatcct tcatcgcctg gatatgcgct
     9121 gccaggtaat cgattcggta atcatcttcc acataaccat tttcatctgg cgtatcgact
     9181 gcacctaagc cgttttctac aataaataat ggtttttgat aacgatcata aatatcattc
     9241 atcgttattc tcaagcctaa tggatcaatt tgccaccccc attcacttgc ttgcagatat
     9301 ggatttttga cagaagcaaa gatatttcct gcagtctgtt ccaagagttc gttgtcggta
     9361 gtcgaaactc gtgaagaata ataagaaaaa gagataaaat caacagtatg ttctttcagc
     9421 aatgcggcat cccctggttc catttcgatc tgaatgccat ttcgctccat ttctttcaat
     9481 gcataagcgg gatattctcc tcgtgactgg acatcaatga agaaataatt ttctcgatct
     9541 gattttctag aagcccaaac atcttcagga ttgcaagtgt atggataata tgctcctgct
     9601 gccagcatac agcctacttt gttctccgga tctacttcat gagcaatctt cgttgcgatg
     9661 gcacttgcaa ccaactcatg atgtgccgct tgatatttga tttgttcttc attttctcct
     9721 tcttcaaaat acagccctgc gcccataaat ggtgcatgaa ggatcatatt gatctcattg
     9781 aatgtcagcc aatatttgac aagtccttta tagcgattga agatgactcg acacagattt
     9841 tcataaaaac caacgagttc tctggaacgc cacgcaccat attctttgat caaatgcatt
     9901 ggacaatcaa aatgcgtaat cgtcacaagt ggctcaattc cgtgcttttg gcattcttta
     9961 aagacatcct cataaaattt caagccagct tcattcggct ctttctcatc ccccattgga
    10021 aaaatacgac tccaggcgat agacattcga taagtcttga accccatttc tgcaaacaaa
    10081 gcaatatctt ctttataacg atgatacata tcgattgcaa tttttgctgg ataaaaatga
    10141 tcctcatcaa attcaaacat ttttttcttt ccggtgataa caggaaaacg gtcttttccg
    10201 attggcacaa catctacgtt tgctagtccg cgtccacctt catcataagc gccctcacat
    10261 tgattggctg ctgtagcacc gccccataaa aaatcttttc tgaaccccat tatacttcct
    10321 ccattatatg tgtattacct gataattctc ttgccagttc atcttgaact tcgattcctt
    10381 cttcttttaa tcggctgatt gtttccttga attgcggtaa atactgctgg tgtgcaataa
    10441 acaactcatc catgatccgt tttgctgtct ttcctgatcg tataagtgga ttgatcgtaa
    10501 acgcctgtaa agctgtccca taatcaccag tcacagctgc ttcgatcgtc agcaattcca
    10561 tattcttcat tacttgcaac cagcctcttt cagcagcagg aagagaacca aacgctacat
    10621 ttctcgctcc ttgtgcgcca acataggcag tgacttctac aacacaatct gccgacaggt
    10681 caggaacagc tccatcattc tttgtagata caacgatctg tgtttcttta tttccataga
    10741 ttgaagcaat cgtttcacag gcagcgtctg aataataagc gccacctcgt ttttgcaatt
    10801 gttcaggttt atggtctaat tctggatctt tgtataattc aaatagctct gcttctgttt
    10861 ctttcacttg ctgcgctctt gtaccaatcg tttgatattc ttctaaagca tgttccagca
    10921 tttcttcttc acgatagtaa tagcggtggt agccacaagg gatcatgttc atttgtttta
    10981 attgttcttt gaaaaacggg atatcaaaga tatttttcgg taatccatta tcttcatcgt
    11041 ataaacgatc aatcaattct gccgtgcggt catttccttt attatctgct actttgtgcc
    11101 aatggaaatg atttagaccg gcaaacttgt agatcagctc ttcttttttc ttgccaagca
    11161 ttgctggttc tgtcatcatc gccatcactg gcacattgca taatccaatc actttatccc
    11221 atttgccata tctgataact gcttctgtca ccatcccact tgggttcgcg aaattgatca
    11281 gccaagcatt tggacataaa cgcttcatgt cctcaacgat tgccaaaacg actggaacgg
    11341 tacgaaaagc tttgaatatc ccgccagcac catttgtttc ttgcccaatc atcccataat
    11401 aactagggat ccgctcgtct ttgattcgtg catctaattg accgacacga aactgagtcg
    11461 taacaaaatc cgcatctttc aatgcttctt cccgatccag agttgcatag atcttgactt
    11521 catatggaga agcatcccac atacgctgag acatctgact aacgatctca agcttttctt
    11581 ttccttcttc tacatctacg agccagattt ctcgtatcgg taattcctca tatcttttga
    11641 taaatccttc catcaactca ggggtgtaac tgctgcctcc accaatcgta gcgattttga
    11701 ttccttttgc catgttctca ctcctttact tttttacact ttcattatct tttttgtaaa
    11761 caaaacaaac aatagaaatc cgtttctttc cagtaacatt tacaaaagtt actgttttct
    11821 agtggatttt tgtaaatatc ttgtcaaaaa aagcctattt taagttatat agtataaatc
    11881 cgtttacaaa acattttcaa aaaagttata atttacgtaa aaaaataaga agaaggtatc
    11941 tacatgttat tacgtgaaaa aatcaaagaa atctcctttt ctccttcaga aaatgaagtg
    12001 atccagtttc tcttaagcga ttctccagca cttggcgaaa tgaccatcca agcgatagct
    12061 gagcgatgtt atgtccaccc gtctacactc atccgtgtag caaaaaaaat gggttttgat
    12121 ggctgggtca gttttcgtaa tgctttttta gctgaaacag cctatcttga agggaatttt
    12181 caagacgtag atgcgaatct tcctttttcc accacagacg gtattatgac aatcgcaaat
    12241 aaaatggctt ctttagaaca gatgacgatt catgatacac tttctctcat tcatcatgac
    12301 caactgcaaa aagctaaata tttactgctc caggcaaaaa aaatcgtgat cttcgccagt
    12361 aactcgaata ctttgatttc tcaagatttc atgctgaaaa tgaagcgcat caaaatggat
    12421 gtcacaattg tcaccacttt tggtgaatca ttctatgaag cgtacaattg tacgagcgat
    12481 atgtgcggga ttctgatttc ttatacgggt gaaaacaatg tgatccttcg taccgcagaa
    12541 atattgaaac aaaatgatgt tccaatttta gctttgacca gtatcggtga ttcaacgcta
    12601 tctgcaatga gtacagccgc tttgcgactt acgacacgcg agcgactgta ttcaaaaatt
    12661 ggtaatttta cgatcaatac atcaatttgt tatcttctag acgtgttata tagttgtgtt
    12721 tttgcagaaa attatcaaca gaatttagtc catctaattt ctgtcggtca aaaattcgat
    12781 aaacgcccta tcagctcagc catcatgaaa gagcctttca attttgaaaa taaagaagtg
    12841 gaataaaaag aatagttaag cctagtccat tcttcaccta accgttcagg tcagtgcggt
    12901 gataagtgca tcgtctggcc cgaccgtctc cccttggttt tccaaaatat ccaaataatc
    12961 cgtgtaattg gtgacgatca caggtgtttc taaactgtat ccttcctttt cgattttctt
    13021 tttgtcgaag cgtagcaaca gatcgccttg tctcactctt tctccttgct gcactaagct
    13081 ttcaaaatat ttcccttcca actgcacagt atccaatccg acatgaatca gcaattccag
    13141 ccctgtttca gaaaccattc cgattgcatg tttcgtcggg aacagggtca taactgttcc
    13201 gtcaaaaggg gcacgtactt ctcctttttc agggtagatt aagattcctc gacctagtgt
    13261 tccttgtgca aaagctgcat ccttcgctgc atttagtgga gcgatttgtc cttgtatcgg
    13321 tgtttttaca atttctttac ctgatttttt ctgtatagca tctgtttcac ctgcttcatc
    13381 cttccaaaag aaaaaagtca gcgaaaaccc gattaccgaa gcaatagcaa tgccgataaa
    13441 cgcttgaaac ataccactgg cgtctgtttc cgtgatgtag tttaccaccc caaaaatccc
    13501 taatccgccc attatgtagc tgattgttcc agatgccatc aaatagattc ctgaaacagc
    13561 accaccaatc atcgagtaaa taaatggctt cttcttcggt aaggtgattc cataaatagc
    13621 cggttctgtt acgccgaaga ttcctgaaat cacagctggc atgcaaagcg ccttcatttt
    13681 ttgatccttg aacttgaaat acatcgccaa taccactgct gtctgtgcaa aactcgcgcc
    13741 ataaacgcct actaacatct gactccaccc attttgcgct agctgcataa tcgcaagagg
    13801 gataatactc caatgaagtc cgaaaatcac cagtacttgc caaagaaacc ctagaactgc
    13861 accatacaca gctggagaaa agtccatcaa tgcttggaat cccgtgctta atagatctgt
    13921 cagtatactg acaatcggac cgataatcaa aaagccagca ggaagtgcga ttaataacac
    13981 agaaaaagaa accaaaaagg tctggacgac tgcaggaatc catttcttgg caattttttg
    14041 gacttgtccg gcaaatgcga taatgaagat aacaggtacg acgctactag tataactgct
    14101 gcccacccat gggataccca aaaatgtttc ataagccgga agacccagca agctataagg
    14161 cgcaggactg ccatcaggga aaccactttg tagtgctgct ccttgtacag tcggataaca
    14221 aagtgctgca ccaatcacga gccccaccat cggattcaga tgaaattttt tcgctgctgt
    14281 gtaccctaag atcactggca tgaaataaaa aattgcatcc cctatcccat tcagcatgat
    14341 gtacgtaccc gatccttcag tataaaattt gagaaacaag aacaaagcat ttaatccttt
    14401 gaccattccc gctgcagcca aagcacctaa aaaaggctgg aaacagccac ttaatatgtc
    14461 gattaatcga ttgaacagat tactacttga agaaacctct tcttctgctt catcggaaat
    14521 cccggctact gtcagtactt ctgcatagac atctggcaca tgattcccga ttacaacttg
    14581 atattgcccg ccgcttttca tgatggtgac tatcccttcc atctttttca aaacatcatc
    14641 ctttgcttta ttttcctcct ttagtttgaa acgaagtcgc gtcacacagt gtgtcaagct
    14701 acggatattt tcttttcctc cgatattctt tacgatgttt tctgctaatt cctggtattt
    14761 tcccatttct attccctcca attaattagt ggaaggttta gccaactgaa tgtcactacc
    14821 cttatttcat acgttcttgt taatttgtcc tcctttttta tttatttaca acgattctca
    14881 tgcactaaac gcgcagtatg gatcgttaaa tacatttttt catccgtgga catctggtag
    14941 tcgaaagccc cggttaaaaa gtctgctatt ttctcaacgc aaccaaacgt atccttatat
    15001 tttttttgaa taacttggaa caattcctca gaaacttctc catcttctga tttcgctcca
    15061 tctaggacac gtgtcagaaa aaaacgcagg tgtgtgacga atcgaaaaaa gtggactgaa
    15121 tcttcatcaa atgtaatctt atagtaatag cggacgatat ttaaaatatc ctgtattgtt
    15181 gtggtaaaca ggtaggtatt gtcttgctgg ctttgaatgc cttgtgcatt gactaaatgc
    15241 aatgcgataa atccggcttc gtcttctgat aacttgatgt ggtatttatc cgagattttc
    15301 ttcactgttt ttttcccaat ggcatactct ttaggaaaca gttgtttgat atcatacagc
    15361 aatacatttg gcacaaagat ccccattttt tgacgttcga tagctgtata caagtgatcc
    15421 gtcaatgaaa tatacacaga atcatttaaa tggacttgca gagcctcttt tgtatattca
    15481 atgatttcac aagaaatttc taagtactca agaggaatcg cttgtgccaa ttcttggaac
    15541 tgttgtgaaa gtccgtcatt tgtcaactga aattttttgt cgatgcatga gtcaagaatc
    15601 gggtcaccca ccttcttttt gaatgccaat ccttttccca tcacaatcat ttcccgatct
    15661 ctttcatccc ttgttattac gacgttgtta ttcagtactt tctcaatcag catgttttgc
    15721 tcctttccat gatatatagg gctgtatcca aaacaaaaag aaaaacccaa aaagccgtcc
    15781 atgaaaccat gggagactca tcaggtttag cttgcatcag gcaatcacta tccaagttgt
    15841 atcgttttct ttatattatc gaaagcaaac gattttgtca acgctttctt aggggctatt
    15901 aggcacttct gactagctga cgtcgtcttt tttaaatctg gtaactacga cactaactct
    15961 cacagatgaa aaatatggga agctatgttt tattttcact ctagtgctta ggtcaaaatg
    16021 ctactgtcac aacatctttt atataccact actttgcagt ttgtacatat ctgcatacaa
    16081 accattttgc gctagaagtg tttcatgatt cccgcgttct actatatgac ctttgtctaa
    16141 aactaaaatc aattctgcat ctcgaatcgt tgacagccga tgggcaatcg caatcgttgt
    16201 ccgtccttgt cgcattttcg ccaatccttc ttggatcaat ccttctgttt cagtatcgat
    16261 atttgctgtt gcttcatcca atactagaat tttaggatca gtcacgattg ttcgcgcaaa
    16321 agtgatcagc tgtcgttgtc cactagaata gctggcacca tgttcgatca cttttgagtg
    16381 atatcctttt ggtaattcat cgatgaattt gtttgcttga acaaactcag ccgcatggcg
    16441 tatctgttcg tctgtgatag ctggattcat catccgaata tttcctgcga tatccccata
    16501 aaacaagaaa gcatcttgca agaccagccc cattttctta cgtaactctt ccattggata
    16561 ttctcgaatg tcatgatcat caatcaatat ctgaccttca taaaattcgt aaaaccgcat
    16621 caacacattg atgatcgagc ttttcccact tccggtgtgt cccactaatg caacggtttc
    16681 tcccggatta gcaacaaaag agatattgtt taatacattg tgctctccat cataagaaaa
    16741 gcttacattg cgaaactcaa tcttacctga agtaatcatt tgatctgctc cagggttttg
    16801 ttgtggtgca tattcttccg tatccataat cttcaagata cgactaccag caaccatccc
    16861 atctgtgaag atgctcaaaa agtccatcat ttgagacatt ggattaaaaa atccctggac
    16921 ataagtcgta aatgcataga tcagtccaac ttctacggga gaatgcaacg catcgatccc
    16981 aaataaagtc aacgccaacg caatcgccaa tgcatataga aaattgatga tcggacttaa
    17041 cagaatcgaa ttcgttttga tcatcgcata gcgcgtgtct aaatactcat tgttcacttg
    17101 ttcaaattct ttagctaaac gagcttcttg gcgaaactgc tggataatac gcatgccgga
    17161 aatcgattca ttcaattttg tattcagctg acttaatttt tcccgcatat ttcggtaaat
    17221 acgagaacta aatttctggt aataccagat gacaattcct aaaatcggca agaacaccaa
    17281 agtccacaag gcaatttctc cattgatttg aaacatggca atgaatgaag aaacgactgc
    17341 aaagatccca gtcaatacca tcaaaaagac gtaccaaaat tcaaatagcg tttctgtgtc
    17401 attcgtcact cgagaaacgg ttgatcctgc cggtgtctga tcaaaataac gcatacctag
    17461 tgtatgcagc ttttcaaata attttaccct aatatactga taggtcttca atgaagccat
    17521 ggaatacaaa taccattgga aaaaccaaat gatacttttt actaggactc caaataagta
    17581 cataccagca aaaaacaaaa taacttgtgt cgtagccgac tgtttagcca gatgatcatc
    17641 cataaaagtt tggatgatcc ttggcaatag gacattgatc acagacagag caaacgcaaa
    17701 taagatagca acgatgaacg ttgttctaaa aggtttggcg aacttcatca gtcgtttgat
    17761 gatctttttc tgttctttaa gtggaatcga ttttgtccat tccgattgat aattttcatc
    17821 catcaggcct cgcctccttc tatcttcgct tctaattgct gtttctccca catttgttca
    17881 taccagccgc ctagtgcaag cagctcatga tgggttcctc gttcaatgat tttcccttga
    17941 tcgacaacaa tgatttcttt tgcatgcatc acgctactta aacggtgagc tgcaatgata
    18001 gttgttttct cttttcttat ttcttttaaa ttatgcaaga tagcttcttc tgttttggca
    18061 tctactgcag aaagtgcatc atctaatatt aataattcag gatttttgat caatgctcga
    18121 gcaatcgaaa tcctttgttt ctgtccgcca gataaagaga ctccacgttc tccaactaag
    18181 gtatcataac cttttggtaa ttccttgatt tcattgttga taaatgctaa agaagccgca
    18241 tgctctactt cttcttctga aagttttgga ttagaaaaac ggatattatc tcgaatggtc
    18301 atcgaaaaca agaagtgatc ctgaggcaca tagccgattg atcctaataa agcattcaat
    18361 gaatattctt tgatcgaacg tccgccaaat tggattttcc cttgataatc atcgtattca
    18421 cgcatcagta atttgatgat cgttgttttt cccgcaccag ttttcccaac aatacctaat
    18481 gtttctcctt cttggattgc aaacttgatt tgttctaatg ctggtgattc ttctttcgga
    18541 taattaaaag aggaaatagc tacattcagc tcgcctttta ccggggtctg cactgcaccc
    18601 agttgttcca cgatatgcgt tttttcatgc aaaagttcat tgacgcgatc ataagaagca
    18661 tttcctcttt ccaatacgtt gaacaatcga ccaatcgcaa acatcggcca aacaagcata
    18721 ccgatataac taataaatga gaccatctgc ccgatcgaga tcgtgccttc caagacatac
    18781 tttccaccta aaataattgt tgcaacataa gacagaccga taatcaaggt aatgaatgga
    18841 tcaaataagg agtctagaaa attgactcgt ttgtttttaa taattgcatc ttcaatcttt
    18901 tcttcaaaat ccgctagatc ttctttttct tgtccaaatg ttttaatgac tttgatccca
    18961 gtgatacttt cttgtgtctt atcattgata ctagaaaaag cttcttgcga atcacgaaaa
    19021 gcatcatgga gtttcgaccc cagcactcgt gaagtgacgg ccagcaaagg cagaggaatc
    19081 agcgcaatca gtgttaagcg ccaatcaata aacaaaatca tcacaatgat cgttgttccg
    19141 cccgtaatca ctgaatctgc aaaggtcaag ataccagctc ctgctacatt ctggatcgca
    19201 ttcaaatcat tcgttgcatg agccatcaaa tccccagtcc gatatttttg gtaaaagaga
    19261 ttgtccattt ttgtaaaatg tttgaataac tgctgccgaa gttctttctc caaccgagct
    19321 gcacttcccc aaatattcgt ccgccagata taacgaaaaa gatattgtaa gaaagcagcg
    19381 ataatcaata ggaagatcca cccgaaaatg atctttactg ataaattttt atcggcaatc
    19441 tcatcaatga tgatcccaat cacttttgga ggaactaact gtaccaaggc cactgcgaat
    19501 agcgagaaaa ccccaattaa ataatgtttc ttttcctgac gaaaaaacca gcctaacttt
    19561 ctaaaaatcg acattttact cctcctttgt ctttatcaag agcctaggat tgtctgtgct
    19621 tgtctttttg ctgaataatc tgaatagtct aacaaattac aaaataaaaa acggactgaa
    19681 aaaatcagcc cgtaaaaagt cgttgggtca gaacctattt tttcccttgg ttcttcattt
    19741 gctgcatcat ctggcgaatt ttcttctcag atggtttttg tcccattgac atcatcattg
    19801 atcgcaacat gtcctcatta acgggaggat ttttttctaa atagtctttc atatatttac
    19861 gcgctaggaa aaagccacct actgcgccga ttagcaaagc aataatagca ataagtacga
    19921 caatacctgt tgataacata taaatctctc ctttctattt ccgaattgct ctcaatgact
    19981 tattttacta aagtttattg aaaaaagaaa ggcgtattct ttagtttcat gcaaaaaata
    20041 gtggtttttt tagaggagaa caaaatttct ttcgtcatct aacaagacgc gccacgaaaa
    20101 taaattaggc ataccgaata tttttcttta attttttctt tttgaggtgt agaatgtaat
    20161 cgtagataaa taggaaaggt ggttttttct tgaataatca atccaacaaa caatttcata
    20221 agcatcgact tattgaacat acaaatggtt tatctttagc tgaagtaaat ggaacagttg
    20281 atgtaccaaa ggggaaaagt ttttttaaaa cacttcttgc ttactctgga ccaggtgcat
    20341 tagtagcagt tggttatatg gatccaggta actggtccac ttcgatcact ggtggacaaa
    20401 atttccaata tttattgatg tcagttattt tgatgtctag tctgattgct atgcttttac
    20461 agtatatggc agcaaaatta ggaatcgtga cccaaatgga tttggcccaa gcaatccgag
    20521 cacgcacaag taagacattg ggtgtcattt tatggatctt gactgaatta gccattatgg
    20581 caacagacat cgcagaagtc attggtgcag caatcgccct ctacttactt tttgatattc
    20641 cattggtgat tgccgtcttt attactgtat tagatgtttt cgttctctta ctactgacaa
    20701 aagtcgggtt tcgtaagatc gaagcactgg ttgtctgctt aattttcgtc attttattcg
    20761 tttttgtcta tcaagttggc ttgtctaacc cagattggca cagtttattt gctggcttta
    20821 tccctaacag tcatacattc tcagactcac cggtcatcgg cggcgaaact ccgctgactg
    20881 gagctttagg gattattggt gcaacagtca tgccgcataa cttatatctt cattcagcag
    20941 tttctcaaac tcggaagatt gatcaccaag acgaagaaga tgtagcacaa gctgtacgtt
    21001 tttctacttg ggactctaat atccaattgt cgatggcttt tctagtcaat gcactgctat
    21061 tgatcatggg tgtcgctgtc ttcaaaaccg gttctgtcca agatccttcc ttttttggtc
    21121 tgtatgaagc cttatctgat ccatcagcta tgagcaatgg tatcttgatt gctgtagcaa
    21181 aatctggtat cttatccact ttatttgctg tagcacttct tgcttctggc cagaactcta
    21241 ctattactgg tacgttgacc ggtcaagtca tcatggaagg cttcatccat atgcgtatcc
    21301 cgatttggct aagaagactg gtcacacgat tgctttctgt gattcctgta ttgatttgtg
    21361 ttttgtttac aagtacaaaa ggaacgatcg aagaacatac cgctttgaat aacttgatga
    21421 atgaatccca ggtgttcttg gcttttgcgt tgccgttttc gatgatccct ttactgatga
    21481 tgaccaacag ccaagcggag atgggtagcc gatttaaaaa ttcattcatc gtaaaacttc
    21541 ttggctggtt ctctgtgatt agtctaactt acttgaactt acgaggatta ccaggacaga
    21601 tcgaaagttt ctttggtgaa caagccactt ctgctcaact agcattggct gatcacatcg
    21661 cttatgtaat cattgttctt gtccttctcc tgttggcatg gacgattggt gatttatata
    21721 aaggaaacaa aaaatatgaa cagcgtttgc aggaaacggt agaagaaatc gaatagaaac
    21781 tgatttttcc acacaaagac ccttgatagt taggttaaaa atctagctac caagggtctt
    21841 tttatttaga agataaagcc tttttcagat ttaatttact gaaaactagc atgcttgtta
    21901 ctacgatgaa ttgcgttaat ggcacgcctg cgccagtgta gacacttaaa aaataatcct
    21961 ttttttctgg cgcataagaa caaaaccacg agacaccgaa aagaaaaacc aattgtgtca
    22021 gtattaatcc ggtgaccaca atcaaagagg aactataata ggcacgatca gcttttgcaa
    22081 atacggttcc tagtatcata agtaatatcc ctgctattga agcaatcagc acgggaatag
    22141 ccagaacttg gtaaattttt atgatgacat tcgcgacttt tgctgatttt gttaattgga
    22201 aattaggtgt tctcggttca ttactatcga tccaattctc cattagcgga aggtttaata
    22261 attcttgagc ctgtttgact tgttcttgcg ttccgaaact ataatttcct ccttgatgga
    22321 aatctttata tcgccatgca taagcgtatc ctgattgaag gaaatcgaat acgattggca
    22381 gatcactcat cgtctttcca tctcctgatc ccatcagata gaactctttt ttctttttga
    22441 tggttccctt gtgataggct tcttctaatt cttggttgac ttttttccaa aaatcattgg
    22501 tttcttttgc attgttttgg taataaccag attcgcttac cacatctcgc aatgcccaaa
    22561 agatgatatc tccgggtaat tcttttactt ttccaccttg agtccacgcg tgagtcgtat
    22621 ataattcgtc aatacgggta gaaacagtat ggaatgtagg agatgcggct aatgctttat
    22681 ttagtgcagc atgtgagacc catatctcac tgttttcttt attcaagttc gtttcatcat
    22741 ctattcggat cagttgtttc atcaaacgtc caaattctcc actggaacgg tcattgatgg
    22801 ttgcaatacc gtatgttgtc tcatttttat attcgatcca ctgatagacg atggaaaagc
    22861 caactatagg aagtaaaaac aatgaaagtt tcagccattc ccgtttcgag agaagaaaga
    22921 gtcgtttttt taacttcttt acttctttga aagaaaagtc ttgccctagc aagatctgga
    22981 cgaaactgat acccaaagct actacagtaa aaggaagaag ccagatagaa tcttcgcgaa
    23041 tgaaccaaaa gaaaggaaaa gcgacagaaa gccctaaaaa ccatggcagc aattttctgc
    23101 tagaaccgta tttgcggtaa taaatagcta gtaaacaagc gataatgatc tcaacagccg
    23161 gaatcacgag ggcattacga tatgttcgag tggaatattc atgtgtaaaa ctaatcggag
    23221 aaaacaaaaa ataaaaatag atacttaaaa gaagatactt gttcttcacg atcggtttaa
    23281 acgcgagtgc aaccaatata ctggcaaaaa tactgaacat gccgaacaaa acagcataag
    23341 aaagatgcag tttatgtgca agtactaaaa ataagctgta ggagattcct ttggaaagtg
    23401 ttgtagtcgt atactcccct agccatttac cgtccaaaat attttgagca tagcgaatca
    23461 gcagctggtc atcataggtt gccgacatgt ctgttccata gattgcttta ctatataaat
    23521 acaaacgaat aattgtaaat aatagggcta tgccaagaat aaagtatgta tgtttctttt
    23581 ttagttggtt gatcaagggt tttaaagtgt tcagcatctt ttcgtccttc caatcacgtt
    23641 ttgccacttg cttgtgaaac ttttcgtttt tttacttttc taacagtata ccttgtttgt
    23701 ctaaatttaa aaagaggggg gagaagatgc atgatgaaaa agactgcaaa aagtactcct
    23761 ttgtttctta aacaaaaaag gacaaggcaa tgtgcctggt ccttttttgc tgctgtcaga
    23821 tagaaaatct tagagtatca cttcttctgc taataactca ggaaacactt ctggatcgaa
    23881 tatttgtgct ttgaacatgg cgatttcttt tccatatggt gcgtaagctt tctttttatc
    23941 tttgccgaca tagggagttt ccaagatttt cgaaatattg tcaaacattg gatcatggac
    24001 gatcttattc agcgtgtcga atccgattgt tccaaaacca atattggcgt gccggtcttt
    24061 atgcgaacct tgcggatttt tagaatcatt gatatggagt actttaagcc gatctaaccc
    24121 gatgatctta tcaaactctt ccattacacc tgaaaagtct tctttaatat tataaccggc
    24181 atcattcgta tgacaggtgt caaaagtaac ggataatttt tcatttaatt ttactccatc
    24241 gatgatttgc gctaattcct caaatgttcg accgatttcc gtacctttac ctgccattgt
    24301 ctccaaagcg atttgtgccg tctgttcttt tgtcaaaact tcattcaagc cttcaataat
    24361 tctagcaatt ccggcttctg ttcctgcccc tacatgagca cctgggtgaa gtgtaatttg
    24421 agtagcgcct aaagcttccg cacgaacaat ctcttctctc agaaattgga cggcaaaagg
    24481 aaacatttct ggtttgatcg tattccccaa attaatgata taaggggcat gaacgacgat
    24541 atttgttaga ccgttttctg ccatgtacgc ttttccggca tcaatattca tctcttcgat
    24601 ggcttttctt ctagtgtttt gaggggcgcc tgtatagatc ataaaagtcg aagatccata
    24661 actggctgct tctgctgctg ctcctaacag cattttcttt ccgctcatgc tgacgtgtga
    24721 gcctaataac atggatggat tcctccttga aaggcaattg ttactaacaa ttcctatata
    24781 ttggtaagat gatatacaaa gaaaatgatt gatttttctt tgccctgttc tccatagtaa
    24841 ctgaaatttt ctctaaagaa aagcttaaga actcttttat aataaagatc attttctcac
    24901 gttttaactc ttttaaaaaa gaaattcctg ccaaaaacag ccttgtctgt ttttttgttc
    24961 tattatttgc tataattagc gaaataaaac cattagttga ggtgaccctt ttgccacgca
    25021 aaaatactcg aaaaaaacag aatagaagaa aaaaagaaaa atggtttgtt cctaagatta
    25081 tttttagagt ttttcaatca ttgactgttt ttattactgt tcttcttttg atgttcgcag
    25141 ctctaggtat tggaataggc gctggctatt ttgcctatct cgtcgaagac acacaattgc
    25201 cgactaaaaa agcactgcag acagaactag gaaacattac agaaacgtca aagattgttt
    25261 atgcagataa tacagagata tcaaaaatcc agacagatct gatgcgcacg actatttcat
    25321 ctgataagat ttcaccttta ttgaaaactg caattatcag cacagaggat gaatacttcg
    25381 ataaacacca aggttacgtt cctaaagctg ttttacgagc attagtatct gaagctactg
    25441 gtattggctc ttctggtgga tcaactttga cacagcaatt agtcaaacag cagatcctta
    25501 cagatgaaac aacattcaaa cgtaaagcaa acgagatcat tctttctgca caagtagaaa
    25561 aatacttttc aaaagatgag atcattgcta cgtatttgaa tgtctcacca tttggtcgta
    25621 ataataaggg acaaaacatt gctggagtcc aggaagctgc aaaaggaatc tttggtgtag
    25681 atgcaaaaga tgtgacgctg ccgcaagctg cttatatcgc tggattaccc caaagcccga
    25741 tcacgtatag tccatacacg aacacaggcg cgctaaaaga agatctttct gccggccttg
    25801 ccagaaaaga ttttgtttta ttcagtatgt atcgtgaagg ccaaattacg aaagaacaat
    25861 acgaagaagc aaaagcctat gaccttacaa aagactttct accacaacaa atcgcagaac
    25921 agactgacag agaattcttg tattatactg tgatgaatga agcaacaaga atcattgcac
    25981 aacaattggc tgaaaaagac aacgctgata tgtctgattc atcggtcagt gacgcttatt
    26041 atcaaaaggc acagcaaacg atccaaaata aaggatatac aatccattcg acaatcgata
    26101 aagatgtcta tgctgcattg caaaatggcg ttgaaaatta tggatacatg ttagatgatg
    26161 gtgctggcag tcaagtagaa accggaaatg tattgatgga taacagaact ggccgcatct
    26221 acggattcgt tggcggacgg aattacagtc agaatcaaaa taaccatgcc ttcgatacag
    26281 aacgtcaagc tggctcatca atcaaaccag ttcttgttta tggacctgca atcgacatgg
    26341 ggcttatcgg atcagaatcc cgtgtatcag attatgcaac aacttggcaa gaaggagaaa
    26401 atgctggcga gaaaatcgtc aatgctacta acgaaggatc gaacaccttc caaaccgtcc
    26461 gtgagtcact agaatggtcc aacaatatcc cggcttatca cttatatcaa gatgtgttga
    26521 attccggtgg atcgaaacaa tatgcttacg agcactactt ggcaaagatg aactacccgg
    26581 caaatgacaa ttggggcgtt gaatctgctc ctttaggtac agtagatgtc acgacgcttc
    26641 aacagaccaa cggatttcag gctttagcaa acggcggcgt ctatgaagaa ggctatatca
    26701 tcgattccat cacagataac gcaggaaatg taatttataa acacgaaagc aactctgtcc
    26761 ggatctattc ggaagcaacg gcatcgatca tgaatgacat gatgcgtagt gtgatcaatg
    26821 caaaaatcac tacaccattc aaagatgcca tctcaagctt aaacggaaat ctaggaaaag
    26881 cagattgggt cggaaaaact ggttctacca atgaataccg agattcatgg ctaattgtct
    26941 ctactccgtc tattaccatc agtagctggg cagggcacga tgacaatact ggaatggaca
    27001 gcaaagcaag gatacgttcg gctaattatc ttgccaatct gatcaatcaa gcttaccaag
    27061 caaaacctga catttttggc actgatgaga aattcgaatt atcatctgat gtgatgaaga
    27121 aaaaagtatc tggatttact ggtcaaacac ctggaaaagt tactgtcaat aaaaaatcca
    27181 tccaaacacc aggaaaaaca gttgaatcgc tatgggcgaa aaatggaccg aagaaaagcg
    27241 cctttaaatt tggcgttggc ggtaccgatg aaaactatac agattactgg aacaccgctt
    27301 cagcttatgc aagagcgaat caagcaacta aagaatcaga tgagaaagat gattgattta
    27361 atagaataca cagtaataag ggattgtgac aatagtattg tcacagtccc ttattttttt
    27421 gatggtggtg aacaaaatgc agcatctata cacggattgt tttatatacg ataaatgtaa
    27481 tttcaaccac tttctctctt ttttaagaca caaaaaaagg attataacaa aattttgtca
    27541 taaccccttt tcccaaataa acggtgttct cctgccaact ccgaatcacg agtcacaata
    27601 ttcatcaaac atgagatgct gttttttgaa attgctcctt gtaacgcaga gttaaacggc
    27661 aggcacacaa cctctgaatg gttgtccttt tattattttg tagatccttt ttcttcgatt
    27721 ccataaggca agataatcgt tttttcttcg acttcttctt tattcatcat tttcgtcaat
    27781 aggcgcatag ataccgcccc taaatcgtat aatggttgtg tgatacttgt taaacgagga
    27841 cgagctactt ctgtcagtag tgaattgtta cttgtgatga tttcgaattc ttccggtact
    27901 ttcacgccac ggtctaataa accatccaaa ataccgatgg ctaattcatc atctgaaaca
    27961 tagacagctg ttgcaccgct attatggatc cgatcgacta gagatagacc atctttgaag
    28021 ttgtattgtg cttcaaagat caatccttcg ctatatggta ggttattttc gcttaatgct
    28081 tctttatagc ctttcagacg attttgtcca ttgatgggat cgatcaacgc accactgaca
    28141 aaagcgattt ttttattgcc gctttttgcc aatttgttta ctgcatcttt tgttgcggat
    28201 acataatcaa tgtttacgct acctacttgt tcatctggat caatagagcc agctaataca
    28261 acaggagttt ttgaacgtga aaattctcca cggatttcgt ctgtgatatg gtgacccatg
    28321 aagatcacgc cgtccacttg cttagccagt aagttattca atacattgac ttctttttgg
    28381 tcgttgccat ctgaatttgc taagataata ttgtatttgt acattgttgc cacatcatcg
    28441 ataccacgtg ctaaagaagc aaagaacata ttgctgacat cagggatgat tacaccaact
    28501 gttgttgttt tcttacttgc caagccacgc gcaactgcgt ttggacgata atccaagcga
    28561 tcgataactt ccaagacttt ttttcttgtt gctggtttaa cgtttggatt gccatttacg
    28621 acacgtgaaa cagtagccat cgaaacattt gcctctctcg ctacatcata gatagtgatt
    28681 gtttgctttt ccatttgtgt tctcctattc atattttata tgttttctga aaatacgtct
    28741 tacatttcta aaatagaata tcaaataatc tcaagaattg caaccgtttt atctctgttt
    28801 tcatgaaaat ttttactttt ttcagttttt aga
//
LOCUS       DAZBSA010000029        27543 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000029 DAZBSA010000000
VERSION     DAZBSA010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.029"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            194..1933
                     /gene="yfcC"
                     /locus_tag="ACHGMT_001134"
     CDS             194..1933
                     /gene="yfcC"
                     /locus_tag="ACHGMT_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFN6834894.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2090..3274
                     /gene="iadA"
                     /locus_tag="ACHGMT_001135"
     CDS             2090..3274
                     /gene="iadA"
                     /locus_tag="ACHGMT_001135"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFN6834895.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3385..3588
                     /locus_tag="ACHGMT_001136"
     CDS             3385..3588
                     /locus_tag="ACHGMT_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFN6834896.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3689..4351)
                     /locus_tag="ACHGMT_001137"
     CDS             complement(3689..4351)
                     /locus_tag="ACHGMT_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6834897.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4364..5866)
                     /locus_tag="ACHGMT_001138"
     CDS             complement(4364..5866)
                     /locus_tag="ACHGMT_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6834898.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6414..8093
                     /locus_tag="ACHGMT_001139"
     CDS             6414..8093
                     /locus_tag="ACHGMT_001139"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFN6834899.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8220..8699
                     /locus_tag="ACHGMT_001140"
     CDS             8220..8699
                     /locus_tag="ACHGMT_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFN6834900.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8918..9598
                     /locus_tag="ACHGMT_001141"
     CDS             8918..9598
                     /locus_tag="ACHGMT_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFN6834901.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9606..10937
                     /locus_tag="ACHGMT_001142"
     CDS             9606..10937
                     /locus_tag="ACHGMT_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFN6834902.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10952..11914
                     /locus_tag="ACHGMT_001143"
     CDS             10952..11914
                     /locus_tag="ACHGMT_001143"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFN6834903.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            12025..12363
                     /locus_tag="ACHGMT_001144"
     CDS             12025..12363
                     /locus_tag="ACHGMT_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFN6834904.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12442..13149
                     /locus_tag="ACHGMT_001145"
     CDS             12442..13149
                     /locus_tag="ACHGMT_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFN6834905.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13252..14733)
                     /locus_tag="ACHGMT_001146"
     CDS             complement(13252..14733)
                     /locus_tag="ACHGMT_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6834906.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            15042..15578
                     /locus_tag="ACHGMT_001147"
     CDS             15042..15578
                     /locus_tag="ACHGMT_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN6834907.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            15656..17026
                     /locus_tag="ACHGMT_001148"
     CDS             15656..17026
                     /locus_tag="ACHGMT_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFN6834908.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            17093..17428
                     /locus_tag="ACHGMT_001149"
     CDS             17093..17428
                     /locus_tag="ACHGMT_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834909.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(17509..18366)
                     /locus_tag="ACHGMT_001150"
     CDS             complement(17509..18366)
                     /locus_tag="ACHGMT_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6834910.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            18472..18987
                     /gene="tadA"
                     /locus_tag="ACHGMT_001151"
     CDS             18472..18987
                     /gene="tadA"
                     /locus_tag="ACHGMT_001151"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFN6834911.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            19010..19094
                     /gene="ffs"
                     /locus_tag="ACHGMT_001152"
     ncRNA           19010..19094
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHGMT_001152"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            19304..20689
                     /locus_tag="ACHGMT_001153"
     CDS             19304..20689
                     /locus_tag="ACHGMT_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFN6834912.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSCLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            20726..21403
                     /locus_tag="ACHGMT_001154"
     CDS             20726..21403
                     /locus_tag="ACHGMT_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFN6834913.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            21411..22175
                     /locus_tag="ACHGMT_001155"
     CDS             21411..22175
                     /locus_tag="ACHGMT_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFN6834914.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            22177..22836
                     /locus_tag="ACHGMT_001156"
     CDS             22177..22836
                     /locus_tag="ACHGMT_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFN6834915.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(22866..23375)
                     /locus_tag="ACHGMT_001157"
     CDS             complement(22866..23375)
                     /locus_tag="ACHGMT_001157"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFN6834916.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(23487..24485)
                     /locus_tag="ACHGMT_001158"
     CDS             complement(23487..24485)
                     /locus_tag="ACHGMT_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFN6834917.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            24686..25270
                     /locus_tag="ACHGMT_001159"
     CDS             24686..25270
                     /locus_tag="ACHGMT_001159"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFN6834918.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(25347..26522)
                     /locus_tag="ACHGMT_001160"
     CDS             complement(25347..26522)
                     /locus_tag="ACHGMT_001160"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFN6834919.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            26604..26984
                     /locus_tag="ACHGMT_001161"
     CDS             26604..26984
                     /locus_tag="ACHGMT_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFN6834920.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            27058..27420
                     /locus_tag="ACHGMT_001162"
     CDS             27058..27420
                     /locus_tag="ACHGMT_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834921.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         8939 a         4662 c         5753 g         8189 t
ORIGIN      
        1 acagagaaat aaaatactta atataaatgg aaagagaaaa aaataatcat acataggaga
       61 aaaaaaacaa aatagaaaaa agtgattttc taataaatta ttcatgtgga aatgtcattt
      121 ttactgtata attcatttga ttatccattt tttattcatc aaaattactg aaaaaagaaa
      181 ggaagaaata aatttgaaga aaacaaattc ttcaggaaaa ccgaaacgcg acttcactaa
      241 attatcgaca ccccatacgt atgtcattat ttttggtgta gtcatctttg catggatact
      301 gacttttgtc gttccagcag gaaagttcag cacgcaagat attgaataca aagatgcaaa
      361 tggcgaaaca agtactcgta ctgttttacg tcaagattcc ttccgttatg cttacgagct
      421 ggataagtcg tatgtgtttg atcaattaga agaactgcaa gatcatccag cagaacgtga
      481 aaaactggat gttccggaaa aaggattgga aaaagtcatt gctgacggag aaaaaaatct
      541 tacgcaggaa aaactagatg aaatctcctt gacagatgat gtgctttacg atcagtacgg
      601 cgaaaatatc tatgatactt ctaaaaaatt gcataaaaca gccaaaatat ggggaacaga
      661 tgattttggc ggctttggtt tcttaaattt tgtttttgaa ggactagtat ctggagataa
      721 gtatggatct gctgtaggga tcgctgcatt gatccttgtt gttggaggtg cttttggtat
      781 tattatgcgt accggagcga tcgacgcagg tatctatgcg tttatcagta aaactaaggg
      841 actggagcgt ttagctttac cgctattatt ctttgctttt tcattcggtg gcgctacatt
      901 cgggatggca gaagaggtaa ttccattctc aatggtgatg gttccatttg tcattgcgtt
      961 aggatatgac tcgatcgtag cagtaaccgt gacttatgtc gcatctcaag tcggaaatgc
     1021 gacctcttgg atgagcccgt ttagcgtagc ggtggctcaa gggattgctg gaatccccgt
     1081 gttgtctggt gcgacattcc gattgattat gtgggtagtt gttactgcat tagccgctgg
     1141 ttatatgatg atatatgcag aaaaaatccg taaaaaacca gaaagttcgt tgacttacaa
     1201 atcagatgat tatttccgtt ctcatatcaa aaagacatct gatgaaaata aaaaattcat
     1261 gcttgggcat aaattgatct tattagagat gttagttgtc cttgtctgga tcgtttgggg
     1321 cgtgactcaa aaaggctatt acattccaga aatcgcttct caattctttg tgatgggatt
     1381 agttgctgga atcattgctg ttcttttcaa attagatgga atgaaagtca atgacatcgc
     1441 ttcttctttc caatctggtg cagctgattt agctggtaca gcaatcgtag taggtatggc
     1501 caaagggatt cttcttgtct tgggaggatc tgatgcatct gttccttcag ctttgaatac
     1561 gattttgcat gggatcggga cggcgttgac cggtgttcca gctgtcattg gtgcttgggc
     1621 gatgtacatc ttccaaagtt tgttcaactt ggttgttaca tcaaattcag gacaagcagc
     1681 tttgactatg ccaatcatgg ctccactggc agatttagta ggtgtatcca gacaagtagc
     1741 agttcttgct tatcagcttg gttcaggttt tatggacgct tttacacccg tatcagccag
     1801 cttgattggt gtgctaggtg ttgcacggat cgaatgggca aaatgggcaa gattccaaat
     1861 caagatgcaa ggattcctgt ttgttttagg aactattttc atcatgattg ccattgcaat
     1921 aggtctgcag tagtactgga gacctgtaaa taaaaaagta atccaaacag tagataagga
     1981 ctccaagaac cgtcatttta cggtaaaatc aaaggagtaa agatcctatc gtttggatta
     2041 ttttttaaga tcctatcatc agttatactt aaagaaagag gaaaaatgta tgaaaatcat
     2101 tcgacaaatt gaagtgtttg caccagatta cttaggaaaa atggatgtac tgattgccgg
     2161 tgggaagatt ttggctgtag aagaacagct agaaggcggc tacgaaggag tggaagtaga
     2221 agaactttca ggtgagggaa aagtactaac cccgggtttc attgactgtc atttccatat
     2281 tttaggtgga ggaggagaag gcggttatca aaatcgaacg ccagaagtca cattgagcca
     2341 attgactaca gctggtgtaa cgacagttgt cggttgtttg ggaacagacg gagaaggtag
     2401 agatatgact gcgttgatca gcaaagcgaa aggtctggaa gcagaaggaa tctccactta
     2461 tgtatacgaa ggttcgtacc gattgccggt aaaaacagta acagattcta tcatcaaaga
     2521 ctttttgaca attgataaaa ttatcggaat cggagaaatc gcagtatccg atcaccgttc
     2581 ttctcagcca agttttgaag agttcgctcg tgcagtagca gatgctcgtg taggaggcat
     2641 gttatccgga aaagctggga tcatcaatgt ccacttaggc ggaggaaaaa gaaagctaga
     2701 gctattgaca cgaatcgtag aagaaacaga aattccgatc acacaatttt tgcctactca
     2761 tgcaaatcgc acaccagaat tatttgaagc ttgcgtggca tttgccaaac gtggtggcac
     2821 gattgacttc actgctagcg aagatcctga tttttgggag aagacagatg gtgaagttcg
     2881 cttcagcaaa gcactgaaac gattgatcga ggaagatgtt tctttagata atttcacgat
     2941 gacctcagat ggacaaggaa gcttgcctta ttttgatgaa aacaatcatt tcctagggtt
     3001 aggagtagga agtgccaagg cattgctagt agggatcaaa gaagctgtgc aaaaagaaag
     3061 tatccctttg gaaatcgcct tacgagctat aaccagtaat ccagcacgta tattgaaatt
     3121 ggataaaaaa ggaaaaatcg aaattggtgc tgatgcagat ctgtgtatct tggataaaga
     3181 aacgcttgat attgatacag ttatcgctaa aggagaaata atggtccaag aaaaagaagt
     3241 caaagtatgg gggactttcg aaaaaagttt ttaatcaaca gctctaacta tcttttttag
     3301 aaaacgagcg ttctgtttgt ctgtatctgt tgttgatgtt accctgaagg tagatcaata
     3361 aacaaactaa gaggaggaag cacaatggca gatttaaaag gacgttctaa agatatgaaa
     3421 gataaagtag tcggtgaagg aaaagaagca tacggcaaag caacagacga caagagcaaa
     3481 gaaacagaag ggaaagcaca atcactaggt gctgacatca aagaaaaagc tcgaaattta
     3541 ggcgacgaag tcaaagacaa gttttctcga aaagacgaaa aaaaataaaa accacttatt
     3601 actaattgaa tcaagcaaat aaaaaatccg aaccatatct gcgagacaga tatggttcgg
     3661 attttttatt tgatcattat cctgtttttt aaacttcgat tttttgaaat tttttatttt
     3721 ttagttcaaa gacttcatca cagctttttg ctacttcaat ctcatgagtc acgatgataa
     3781 tacagcgatt ttgttcatgg gcgatttctt ggaaaagttt gacgatatct tgtgaagttg
     3841 tttcatctag atttccagta ggctcatccg ctacgattag ttgatgttca cagcagatag
     3901 cacgaacgat agcgactcgc tgctgctgac ctcccgaaag tttggtcacc tgtttttttg
     3961 ctaatatttc atcaattcct acacgagcca gtcgatctaa agcaacttgt cgataattct
     4021 cttgcttggt cttagtgatt tccattgctg ctatgacgtt gtctagagcc gataaataag
     4081 gaagcaagtt gtaagcttgg aaaataatcg agacatcatt tttacgataa cttcttaacc
     4141 cgattttgtt cagtgaacga tcttgatagt atacctttcc ttcttttggt gcatctagtc
     4201 cagaaataag cgaaagaaaa gtcgtttttc cagaaccact tgctcctaaa atagcgtaca
     4261 tttttccagc ttcgaaattc agttctacat ctttataaag cgcatcttgc ggatcttgat
     4321 accaaaatcc caattgttct gtccgtaaca tcaaattcct cctttagttt aataggattt
     4381 ttcttgggtt taatcttaat atccctatag aagctaaaaa gacagaaaac agactgattc
     4441 caaatgctac tccggctaat agtcccaatt gtgcaggttt gacattgatt tccaattcgc
     4501 tgacttgatc agaaacagca aatggattat tcgtggttcc ttgttgacca tcgcgttgac
     4561 cgccttgttc ttgtctgtta cctggcattt gtccaccgcc aggagcttgc tggttttgtg
     4621 tcgtggtttc tgtctgttgg tcaagtagct gctgtcccac ggcattcgcg acgacgtttc
     4681 cgctaagcga agcaactcct aatgcaagaa tcatacaaat agccacttcg gtaaacaatt
     4741 ggagaatgat tttacttctg gattctccta aagatagcaa gacgcctaac tcatgccgac
     4801 gctcccgaat ggtaatcata atgatcagag taagtatgat aataccggct acagccacta
     4861 gtaaaacaac atttttagaa aaacttgcca cgttgtttaa aggttccaac attgactgat
     4921 acatggaatc atttgattgt aagctaaatg tatctgtgtc gatcagcttc tcagcttctt
     4981 tgagaaactc atccatttta tctggatcag atagagtgta aatagcagaa tctatcgtgt
     5041 tatcgtcaga tgtcccattt agttcatttg caaaagtgta agaagtgtaa agtgtattcg
     5101 acggattcat gaagttgaat ttcataccca tactgccgct tgtttcgctg gattcataaa
     5161 ttcctttgat cgtcatttca taagtggtat cttcatcttc agtactcgtg attgtgaaag
     5221 tatctccgac tgccaggtca tttgtttctg ctagagtaga atcaatgact acattgtttg
     5281 tgcctttgtc gctgtcatct attccttcac catccgtgat ttttgctgtt ccatcagaaa
     5341 aattactatt ttgtgcagtt gctgacacac cactgatttg aaagtctgct tgagtcattt
     5401 gcggattccc gcccattcct ccagcaggtt caccgcctgc atcagtagtt tctgaactgt
     5461 cttcacttga atcagaacta gtgatggcag ttattccatc ttttgccata gctgaggcgg
     5521 aactttcaaa ggaataactc tttacattat ctaattcagc tattttttcc gcgtccgtta
     5581 aggaaactgg tgtaagagaa aaactgcctg gatctggacg ttcttcagtg gtagaaccgc
     5641 ttgaagaagt ttgttcctgt tttttaaatg aagcttctcg attggcactt aaagtcactg
     5701 ttgctccaac ttctttttgt gcttgattcg ctgcttcttc tgcagcgctt cgaatcgtga
     5761 ggccagctag cacaaaaata agaatggctg tgaacacggc aaacaataac aatgaacgtc
     5821 cccattttac ttttgtactt ttccatgcac gtttcataaa attcatattt tgcatccctc
     5881 ctagtagatg catttgataa tagacaacct atcttaaagc tagcttaaat cttatgctgt
     5941 ttgcacacca tcaacatgag gttctgtata tataaaaagt cactatagtt attgcaaaaa
     6001 atgtggtaaa atgaaaaaag tatgtttcaa accgacgaag aagaaatgaa agaggaatag
     6061 caaaagaaat cctagatgct acagagggat ttttacgtgc atagttgttc taacaattgc
     6121 tgatactagc gatttagttt atttgttgta taataatggg gaacagctga atacttatat
     6181 aagtattctg gctatagcgt ttcgcaaaag atccattttg ttaatgaagt atatccaaaa
     6241 taaaatgccc atccaactgt cagaacgcgg atgtgctggt aattttcctg ctgattctcc
     6301 ataagttgag aaatagtgat tttgcatatg cttcattgga atggctcata tatttagata
     6361 aagaagaaaa tcgacatctt taaacttgga tgtcataaga agaggtgaag tttgtgagta
     6421 ccattaaaat tatcccatta ggcggcgttc gtgaaaatgg aaaaaacatg tatgtcgcag
     6481 aagtgggcga ggatattttt attttagatt gtgggctaaa atatcctgaa aatgagttgt
     6541 tggggatcga cgtcgtaatc cctgatttta cgtatttaga agaaaacatt gatcgtgtag
     6601 ctggtgtctt tttaacacat ggacatgcag atgcgatcgg ggcacttcct tacttgttgt
     6661 caaaaatttc tgtaccagta ttcgggacag agttgacgat cgagttagca aaattgaatg
     6721 tcagtcgtaa tgaagcagcc aaaggattta aggatttcca tgtggtagat gagcatacag
     6781 aaatagactt tggtgaaaca gtagtgagct ttttccgtac cactcatacg ataccagatt
     6841 cagtggggat caatctgaaa acaaaagaag gtagtatcgt ttatacggga gattttaaat
     6901 ttgaccaaac ggctatccca atgtatcaaa cagattatgc acgattggcg gaaatcggca
     6961 aagaaggagt cttagcctta ctaagtgaat cggcaaatgc agaaaatcct cagcctgtag
     7021 cttctgaagc ccaaatagca gatgaagtat ttgatacgat caaatattgg gaagggcgta
     7081 tcatcgtcgc ttgtgtagca agtaatttgc aacgagtgca acaagtattg gatgcagcag
     7141 aaaaagcaca acgaaaagtt gtcttgactg gtcaagattt cgaacgaatt attcgcacag
     7201 cgatgcgttt agaaaaactt caattaccaa gtgaagatct gcttgttacg ttgaaagaca
     7261 tgaaaaacta tgatccagaa gaactgatca ttttagaaac tggccgaatg ggtgaaccca
     7321 tcaagtctct gcaaaaaatg gcaaacggta cacaccgtaa tgtcaagatt gaagaaggag
     7381 atttggttta tatcactact acgccaagca tcgcgatgga aaccatcgtg gcgaaaacgg
     7441 aagatatcat ttatcgagct ggtggaacag tgaagttgat ctctgagaat atgcgcgttt
     7501 ctggccatgc caatcctaat gacttgcaat taatgatcaa tttgatgaaa ccaacatatt
     7561 ttgtgccagt tcaaggagag tatcgtgaat tagctgctca tgcagatttg gctcatgcag
     7621 tagggatgcc atacaaaaat atctatatta ctggacgcgg agacgtgctg gaatataaga
     7681 acaagcgtat gagtgttgca ggaactacaa gtgcagaaaa tgtaatgatt gatggattag
     7741 gcataggtga tatcggaaac attgttttac gtgatcgaaa aatcttgtca gaagacggga
     7801 ttttcgtagc ggttgtcact atcagtcggc gagaaaagaa aatcatttca aaacctcaaa
     7861 ttacttcacg gggattcgtt tacgtaaaag ctagccgtga tctgatgaaa gaaagcagca
     7921 atatgattga aacaatcgtt gagaaacatt tagaaagcaa tgattttgag tggagcaaat
     7981 taaagcaaga catccgagat caattaggcc gttacttatt tgaacaaaca aaacgccgcc
     8041 cagtgatctt gccagtcatt atggaagcta ctcaacgtcg aaataaaaaa taaacaaaaa
     8101 gagtaagatg aacctctcta tagagtgctc gtcttgctct ttttttgcga aaaacaaata
     8161 atttttgaat acagcatccc tcgctttata ataaacgata gaataaaggg agtgaatcaa
     8221 tgatgagtca aacaaaagtc gaacgttatt tagaagaaca aaagatcagc tatcaaccac
     8281 ttgattttag tcatgtggta gaagaatctt tcacagaaga actacaaaaa agagggattg
     8341 atccagcttt gatatgtaaa actctcgtag tgaaaggaaa caaaactggg gtgatcatcg
     8401 cattgattcc tttgaacgat cacttagatt acaaaaaaac gagacaagtg acgaaagatc
     8461 gtaaagttgg ttttcctgga atggattttg tattaatgca tacaggatat ccccacggag
     8521 caaacacccc tattggaata aaactcgcac atcctgatta ttgtttttta gcagacagtt
     8581 ctttaaaaga aaaagaagaa gtaattgtgt caagcggtga aatcgggaga agccttcgga
     8641 taaaagtagc cgatttagaa aagatcgtcc atcctattta cgctgattta attagttaag
     8701 ggacgagaag agaagctaag cagagcagag acctcaaact tgagattata tctgcttggt
     8761 acttggtgca tcctgtatta tctgttacaa ttgctttgta catactgtac tatcaatcca
     8821 atgaaaatat atttagcagc tatatttttt cattgatgtg caagtgatga aatcgttttt
     8881 aataaaaaag atacagagaa aacgcgaggt gtaaaatatg aaaattacgt atcatggaca
     8941 ttcctgttta acaattgaaa tggaaaatgg ccaacgcgtg atgattgacc cgtttattac
     9001 aggaaatcca ttgacagact taactgcaga agaagtaaag acagattggt tgctgattac
     9061 tcatggtcat agtgaccaca taggagatat gattcctatt gcaaaagaaa atcaagccac
     9121 aattatcagt attgttgaga ttgctgatta tgcgaacagc agaggagtag attcattcgg
     9181 tatgaatatc ggtggaaaac atgcgtttcc attcggcgtg gtaaaattcg tacatgcaca
     9241 gcatagttca agctatgaag tagacggtat cgttcagtat atgggcgagc ctagcggaat
     9301 tattattcaa gcagaaggaa aaacgattta tcatgcaggg gatactgctt atttcagtga
     9361 tctaggattg cttgcagaag aattcgatat tgatgtcgct ttcttgccga ttggcgataa
     9421 ctatacgatg gggccagaag atgcagcaaa ggctgcgcaa acaattcgtg ccaagaaagt
     9481 agttccaatc cattacaata ctttccctgt cattgaacaa gatcccgaat cttttgtaga
     9541 gttactggaa gatagtgaag gaaaaataat gagcgtctcg gaaacgctgg aagtgtaggc
     9601 agaaaatggc gacaaaacat gatttgattt tagaatatat tgaaagttta cctgttggca
     9661 atcgaatttc tgtaagaagc atcgcaaaag aattgaatgt cagtgaagga acagcgtacc
     9721 gtgcaatcaa agatgcagaa aatgtcggac tagtttcaac aattcaacga gtagggacca
     9781 tccgtatcga acgcaaatta aaaaaacata ttgaacgttt gacatttggc gaggtcgtca
     9841 ggatcattga aggggacgtt ttaggcgggg cggcaggatt agacaaggtc ttgaataaat
     9901 tcgtcatcgg cgcgatgact aaacatgcca tggaacgcta tattacgcca ggatcactaa
     9961 tgatcgtcgg caaccgggcg gaagtccaaa aattagcatt ggaagatggc gccgcagtat
    10021 tgatcacagg aggattcaat acgactgctg agattgcaaa gctagctgat gatcttgaaa
    10081 tgccgatctt acgcacctct tatgatacat ttacagtcgc aaccatgatc aaccgagcgt
    10141 taagcgacca attgatcaaa aaagatatca tgcttgtcag tgatatctat atgccagctg
    10201 aaaagactca ttatttgcag cagacagata cgatttctga ttatcagcgt ttgtcagaag
    10261 aaacgcagca ttcgcgtttt ccagtcgtca accgccatca tcgtttgatg gggattgtta
    10321 cggcaaaaga tgttttaggg aaatcgccaa accagatcat cgatcgggtg atgacgaaag
    10381 aaccaatcag tgtaaagaaa acaatgagta ttgcctctgt tagccatcaa atgatctggg
    10441 atgggctaga agtcatgccg gtcgtttccg atgacttgac attggaaggc ttgattaccc
    10501 ggcaagatgt aatgaaagcg atgcagcttg tccagcgaca accgcagatt gcagatacga
    10561 tttcggatca gatatctgga aatatccata cgattgatac tgaccgtgaa ggaaatcgtt
    10621 tggaaaaccc taagttcaaa ttcgtagtag caccacaaat ggtcaatggc gttggaacca
    10681 tttcttttgg cgtacttagc gaaatcattt ctgatgtcgc acaaaaaaca atggtgatga
    10741 accaaaaacg aaatatcttg attgaacaag tgaacctaca ttatttgcgg ctgatccaat
    10801 tggaaagcga attagacatt cgtccacgtg tattggaaat cggcagacgt tccgctaaac
    10861 tggatatcga agtgtttatt gaaaacacca tcgtttccaa agcaatcgtt gtttgtcaag
    10921 taatggaacg ttcttagaaa gcaggagtga aatgacagta cagcaagaaa tagtagatac
    10981 aataaaaaaa tacgataaaa tcattatcca caggcatttg cgccctgatc ctgatgcatt
    11041 aggatcacaa gtaggattag ccgaactatt acgggaaagt ttcccagaaa aagaaatacg
    11101 tcaagtaggt gaaacgattg aagggcttcg ctttttagca gaaatgcaag aagttgaaga
    11161 tgtcttctac caaggagcgc tagtcatcgt gactgataca gctaattcac ccagaatcag
    11221 tgatcaaaga tatcagttgg cagaaaaact gatcaaaatc gaccatcatc ccaatgatga
    11281 cccttatgga gatcttgtgt gggtaaatac aaaagcaagt agctgtagtg agatgattgc
    11341 agaatttgct ttgatgtttc ctgaatcttt aaagatgaac gttaaggctg cacggttact
    11401 ttatgcagga atcgtcggag atacagggcg ctttttgtat ccctccacga cgtcccgtac
    11461 attagagatt gcttcgatat tgagaaatta tccatttgat gcaagtgctt tgaacagaga
    11521 gattgaacaa atgccgatga aagtcgctaa actttccggt tatctttatc aaaatatcca
    11581 ggtggatgaa aatggtgcag gaagaataat ccttccgcaa gaaattcttg ataaatatgg
    11641 aatcgatgat tcagagacag cttcgatcgt ttcacttcct ggagtgattg acgaagtgct
    11701 ggcttggggg atttttgttc agcagccttc agggtattac cgcgtacgtt tacgttctaa
    11761 ggggccgatc atcaatgaat tggctaaacg ccatcatgga ggaggccatc cattagcaag
    11821 tggtgcgaat gcaaaagatt tagcagaagt agaggaaatc tataaagaga tccaagatat
    11881 atgccaatcg tataagagca gaaaaacaga atgatacttc gttcgaaaac gtgccttttg
    11941 tagtaaaaat cacaaatgta tcttttcaag atcttctttt tccgttacaa tagtgctcac
    12001 taaagatttt aaaggagaaa gaagatgaca ttaccaaatt gcccagaatg tggctcagag
    12061 tacacatatg aagatcgagg cttgttgatt tgcccagaat gcgggaacga atggaacaaa
    12121 gaagaacaag aaactgtcat agaagacgga ttagtggtat tagatgcaaa tggaaaccgt
    12181 ctgttagagg gcgatagcgt gacagtgatt aaagacttga aagtaaaagg tgcgtcaggt
    12241 gccattaaac aaggaacaaa agtcaagaac atccgtttag tagaaggcga tcataatatc
    12301 gattgcaagg tagaaggatt tggaccgatg aaactgaaat cggaatttgt gaaaaagaat
    12361 taaagaatat tcaaggtctt ttcacattct tttcctacta ctaatgataa aataaagtaa
    12421 acgaaaagga gtgaaacgaa aatgtatggt tatggaatgt atggtttttt agatccgaca
    12481 tttcttttgg tcattcttgg attagttatt tccggaattg cgtcagcgta cgtcaatagt
    12541 actttccgaa aatacgatca agtgagaagc aaaaacaatg tgactggtac acaagcagca
    12601 caatatatat tacaaagtca acagataaat aatgtcggtg ttcaacaaat tgccggagat
    12661 ctcacagata actacaattc aggtaataag atgttgagtt tgtctcaagc aactgcacag
    12721 tctacttcag ttgctgcgat aggtgtagca gctcatgaat gcggacatgc ggtacaagat
    12781 gcttcgggtt atgtcccatt gaagattcgt gcagcacttg ttccagcagc taactttggt
    12841 tcgatgattt cgttcccctt gattatggta ggtgtattgc ttagctggaa ctctacgtta
    12901 atcaatatcg ggattttcgc tttttcttta gcattacttt tccaattggt gacattacca
    12961 gtcgaattta acgcgtctcg gcgagcaatc caaattttga gtgaaggcgg actactgacg
    13021 gaagaagaag taccgatggc aagacacgta ttatttgcag cagcactgac ttatgttgca
    13081 gcagcgcttt cgacattcct gcagctgttg cgcttgatct tattgttcgg cggcaaccga
    13141 agagattaaa ataaagaagt tgtgaaatca gcgctttgat ctgagctaat accgagagtt
    13201 agctgattga ataccattta tttggaataa gaggatgtga caagaatttt gtcacatcct
    13261 ctttgttttg ttctcataac cttttttcat atacagttca tgaaccgctt ttattaactt
    13321 gccaaataac actgtattgt gcgttgtatc cagatagaaa gtctccgttt catctgaaat
    13381 tttctcatgc tgatctgtaa tgatcaaatc agcatgttct gaatatgcgg taattttaac
    13441 agcatctttg cgaaataact gctgcaaatg gctttgaaga tgataatttc ctgtaaactg
    13501 tttggttatc tgcaaatgga tgagtaactg ctctttcgtt tctgtttgta gtaagtgttg
    13561 gatcaattga ctaaggtatt gactgatttg gtttctttcc ccattttttg aaaaaattgt
    13621 atcgattttt tctcttaatt tcttttcctg tataaaagaa ctagaatgat tggacgtggc
    13681 ttctggaaag caaagtgtag aaaaactact gctggattca cccaacagtt cgaaaaaaac
    13741 aactgccaac acaagaaaat aaaaataaat tggtatttta ttttctaaat ggttgagagg
    13801 aaactgttga gttaagtaag tcatcaattg accgacttgt cgacaaataa ggtttttgct
    13861 tgtggaaaat aaaaaaccaa tctcctgttt ttgtcgaaaa gagatttgat ttggaaaaaa
    13921 acaatagaaa aaataattaa agtaggctct ctctgtttct gtctcttcac aagggcgact
    13981 cactgaaaaa gaagtaaagt attctgaata atcatgtgta tgctgcaata agtccatcaa
    14041 ttcagtgatt tcagaagaag tttttggaaa atatgcctgt tgtttgtttc gtaatatgaa
    14101 taaagttaac agcaagtaga tatttttttc ttgttttgaa attttttcta tttgctcatc
    14161 tgtactgttc atttttagtg catttaattc aagtgaggaa aatggccaat cgttacctcc
    14221 aaatgtatct gaaagaaaaa agtaaagata gagacggata gataactcat ttccttcaag
    14281 agtacagcaa ttttttacag ataggatccg taaatggaat tttattaagg aatgattgag
    14341 ctgttttgtc agtttttcga gataggaggg cgatatataa agttgttcaa gtaaatattt
    14401 ttttggtaca gcagtttgtg gataattgat tagtaacatc aaatagttaa aacgattgga
    14461 ttcttttagg tagcataatt ttaacttaga aaaaacagct aactttttag tttgatcggt
    14521 ttctttggtt tggatctctc ctttatgatt agaataaata aattcatcaa tcaataaaaa
    14581 ttgcttgagt gatacattta tttttcggat cagttctttg attgtccgtt ttggaatatg
    14641 taagtcattg gataattctt gtaaagaaca attatttttt tgaatgacca tttgtagaac
    14701 aagtaactct ttttttcttg cttcaattag cataaatacc tcctttttat tctgtttttg
    14761 tatatagtta gacaaaaact gtgagtggaa agtcatttca gtctgtgtta ttctcacaga
    14821 gtgtcgcctt cttttttata taacgttatt taattttttt gttaccttgt ttattctatt
    14881 gtttatttcc tagggtattt tgtcaagata ttcttgtttt taaggcggaa aaattgattt
    14941 ttaatggata taactaaata tgttttgata attatcaata atatttttag aattatttat
    15001 gttatttatt tatgggtaga tgaaaagaga cggaggtaag aatgatgggg agagtaacaa
    15061 attatagtaa agaatatttg ctgttcaaaa gtatggtata cgtggaagaa tatggaatga
    15121 agtcaattac tgcaagagag ttagcagagt tctgcggttg ttctacatac ccgatttata
    15181 cacatttcaa atcaatcagc gggttaaaag agaagatatt agaagaaatc actgtttatt
    15241 ttgaaaacta cttagctgaa tgcagttccg atgatgtttt aagcattgtc tacttattga
    15301 aagatttttt ttctatccat gaaagtattc gtgaaatcat tgcaaaatct gatttagaca
    15361 tggattcgct atttaagcgg ccatttttct catatgtcaa aggtcacacg aagatcaaag
    15421 aagaaaagtc gcttgaattg gtatggctta atgcattggg aagtttgcgc tcagatgatt
    15481 cttctcaaga ttttcttgaa atgatgaacg ctgtttggga taaaaacgag gtgacggatg
    15541 aatacttgga aaatatctta aaggaacagt caccttaggc agcaacatga aaaagtagat
    15601 cagccacctt tatttttcgt aaaaaagatt gtaaaatgtc taacgaaaca ttggaatgaa
    15661 ggaggaagcg acaacgttac cacagcaatt tatcaaaaaa tatcgtcttc ttttaggcga
    15721 agaagcatcc gatttttttt ctgcgcttga acaaggaagc gtgaaaaaag gatttcggtg
    15781 gaatccacta aaaccggcag gattggacat ggtccaaact taccatagcg aagaattaca
    15841 acccgctccc tacagcaatg agggatttct tggcactgtc aatggaaagt ctttcctcca
    15901 tcaagctggg tatgaatata gccaagaacc aagtgcaatg atcgttggaa cagctgctgc
    15961 agcaaaaccc ggtgaaaaag ttttggattt gtgtgcggct cccggtggta aatcaaccca
    16021 attagctgct caaatgaagg ggaaagggtt attagtgacg aatgagatct ttccgaaacg
    16081 tgcgaagatt ttatcagaaa atatagaacg atggggagtt tcgaacgcaa tagtgacaaa
    16141 tcatgcaccc gcagaacttg tccctcattt ttcgggcttt tttgatcgga tcgtggttga
    16201 tgctccttgt tcaggagaag gaatgttccg aaaagatcca aatgcaataa aagagtggac
    16261 agaagaatct ccgctatact gtcaaaaacg tcagcaagag attttgtctt cagcaataaa
    16321 aatgctaaaa aataaaggac aactgattta ttcgacttgt acgtttgcac cagaagaaaa
    16381 tgaagagatc attagctggc ttgtagaaaa ttatccagta acgattgagg agattccttt
    16441 gactcaatcc gttagttcag gacgttctga atggggaagc gtagctggtt tggaaaaaac
    16501 aatacgtata tggcctcata aagatcaggg tgaaggacat tttgttgcaa agctgacttt
    16561 tcatggacaa aatcagatgc ataaagaaaa gaagacacga aaaaaaagta aagtgcaaat
    16621 gacaaaagag caggaaaaac tctggacgga gttttcaaat gattttcatt atgaagctac
    16681 tggaagactt ttagtgttca acgaccatct ttgggaagtt cctgagttag ccccttctct
    16741 cgatggttta aaagtcgtac gtacaggttt gcatttagga gatttcaaaa agaatcgatt
    16801 tgaacctagc tatgcattag cgcttgctac aaaaaagata gagaatatac cttgcttgcc
    16861 gatcactcaa aaggaatggc aatcttacac agcaggagaa acatttcaac gtgatgggaa
    16921 tcaaggatgg gttttgctag tattagataa gatacctgtc ggatttggga aacaagtaaa
    16981 aggaacagta aagaactttt tccctaaagg attgagattt cactgagtgg attagttcgt
    17041 ttattttttt gtctatgcta tgatggagat agataaaaag gagctgatac ttatgaaaac
    17101 atcaacaaaa gtaacaatcg gattaagcgt agcagccgct gccagtgtag cgacagcagt
    17161 cgttgtatcg ggaaaagtaa tggaaaagat ccatcatatg actactcgga cgaaagtaaa
    17221 aaaattcgtc tatgacaaat ttgatggaaa tgaaaaatta ctagaaatcg tagatggtct
    17281 ttcagatagt gacttggatt ctttgatggg aatgttagca aaaatcaaat caggaaaaaa
    17341 gaaaatctct gtttatggtg attcaatcaa agattcaacc gaagatgtaa aagaccggct
    17401 aatgcatttt gttgaaaaaa tgatgtaaga gaaactagct tttgaacact attcattcga
    17461 aataaggggt tgtgacaaaa ttattgtcac aaccccttta atctatcttt aattatgttc
    17521 atgaagcaat aaaagatagc aaaacagtaa atcgttggct ttttttagat tgaaaccaga
    17581 ctgttcttga aatttttcga ttcgatattt tagagtatta cgatgaagaa acagtgtttt
    17641 tgcagcagag ctgacattgc ctaattcatt ccataattct tgaatgatca cttccatttc
    17701 attttgtttg atcagttcta tgtaactttt gatcaactta ctatctttta cagtatcttt
    17761 tgtatagtaa tgtaaagcca catcttgcat agaaaaagta cggctgtgag tataaagatt
    17821 gttttgctct gttaaaaaaa tttttctttc ttcagcaaac aaagggacga ggtcggtgcc
    17881 aggtgaatgg aaagaaccaa cgaatacaga cgttgtcgta tcaaaatcag catctaaaga
    17941 tagaaaaata ccgttgattt cagtagcatc aagataatca cggtttattt gttcgactaa
    18001 gagcgcatct gtgtctgtat aaaaaaagaa atcagcagaa tagcgaaata attccaaaat
    18061 agtatcttgc cattcttttt ttaagaattc tccttttgat tcgacgtgaa gctggatcaa
    18121 acgaaagctt ttttcttgaa cacaagaagc atctttaaaa agatagtgat accatgggtg
    18181 ttttttttga tcgttgatta cagggaataa cgctttcaaa aggctggttt cttgttgaga
    18241 taatgaatct tgtttgatcc agataaaagc atcatcaatt gcaaaagaaa ggatatccgc
    18301 ttctgatgct ggagagtttt ttttctgtgc ttgtgggtaa agagcaagta atttatccat
    18361 tttcatcttg ttcacctctt gtatttattt taccatagaa aaaaagttag ttacagcctt
    18421 tttatcatat gctaaagtaa aacaagaaaa aacaaaggca gggaagggta attggtaaat
    18481 acaaatcttg atgcagtaga aaaagaaaaa tggatgcgcc ttgcattggc agaagcaaag
    18541 aaagcagaaa tgcttcatga agttccgatt ggagcagtgg ttgtattgga tggaaaagtg
    18601 attggaagag gatataacct tagagaaacc acgcaagatg ctactacaca tgcagaaatg
    18661 cttgcaatca aggaagcctg tgaaaaagtg ggaagctggc gcttggaaga tgctgcactt
    18721 tttgttacct tagagccttg tcctatgtgc agcggcgcga tgattttgtc tcgtgtagca
    18781 gaagtttatt tcggcgcgta tgatccaaaa ggcggaacag ccggtacctt gatgaatctt
    18841 ttggaggacg agcggtttaa ccaccaagca tatgtagaag gtggcatttt agaagacgaa
    18901 tgtgggatga ttttgactga ctttttccga aagctccgcc agcgaaaaaa gaaattaaaa
    18961 aaagactggt caacgaatga aaaataaggt atactattct ttgccgaaag gctaggacaa
    19021 tggtgggctt gtgaagcgtg tcaggtctgg aaggaagcag cactaagcag gttccgccat
    19081 gtgtctgatt ataatgaaac gacaacagac tttcgggtct gtttttttta tttttatagt
    19141 actgtaaaaa tgaatgttcc tgatttttta gccagactga tttatcgaat attgtactta
    19201 tacaatttgc ttcaaggcag gaaaaactct gaaatagagc ttgtttcaat ggaagaaaaa
    19261 gctgcctatg gtatgataaa gataaagaga ggcgagaggg tacatggaaa aaatagtgat
    19321 tttacatacg aatgatctgc attctcattt agaaaattgg ccaaggatca gaaggttttt
    19381 ggatcaacgt aaacgagaaa acgaaaagaa agaaaaaacg acaacgattg cagtagatct
    19441 aggtgatttt gtggatcgtt ggcatccatt gtcagaagca acgaatggac aggaaaatat
    19501 cttgctgatg aatgaagttg gttatgatgc agcgacgata ggaaacaatg aaggcgtagg
    19561 aaattccaaa gaagagttga atcatttata cgatcatgct gagtttgata tcatcctgga
    19621 taacttattc gataaaaata cgctccagcc accggcatgg acgaaaccat ataaaatcat
    19681 tacaacgaaa caccagacga aaatcggact aattgcattt actgctccat ttccgcttac
    19741 ttataatccg aatggctggg acatacggaa tccttatgat attttgccag aattagtcga
    19801 agatttacgt tcacaagtgg atgttttagt tttgatgagt catctcggga ttcaggatga
    19861 tttccaaatt gcaaaagaaa taccagcaat tgatgttatt ttaggatcgc acacccatca
    19921 tttattcaga gaaggtcacg taatcaatga tgtccaaata gcagccgctg gaaagtacgg
    19981 gcagtatgtc ggtgaagtcc atttaaccat cgatgaagag aaaaaaatcc ttaagcattc
    20041 agccaaagcc atccctaccg aaacaatgac agcttttaca gaagatgaac aagaaataaa
    20101 tagttgtcta gaacgtgggc atgaattatt aaaagaaaaa gaagtagcag atttgccatt
    20161 ttctctgagt ctagatatct tcaatgagca ttcgtttatc caagtagcct ttgaggcggt
    20221 gaaagaacga ggaaagacgg aagcagcaat attaaacagt ggtctgtttt taactgaaat
    20281 tccaaaaggg cgggtcaatc aggatcagct tcacactgcg ttgccgcatc cgatgcactt
    20341 gttaaatgtc acattgggtg gaaatgatct gatccgctta gttttagaaa tagaaaaaaa
    20401 tcgtaacttt ttacgtaact atcctatgaa gggaatggga tttcgcggta agatttttgg
    20461 gcagatggtc tataatggaa tcacatatga ttctattaac catcaagtct tttggaagaa
    20521 caagccaatc gatctgaata aaaattatac ttttactacc gtagatcatt tgatgtttgt
    20581 tccatttttt ccaacgattg agattgctgg aaaaaatgaa tttttgttcc ctgaatttat
    20641 ccgaagtgtg gtaggagatt acttgaaagc tcactaccca atcaaataaa aacaaggtat
    20701 aataaaaaag ataggtggtg aacgcatgtc tgcaaaacaa aatgaacagc agaacgttga
    20761 gcataaaaca gaaattgcta agaaaaaatc atcagctgaa caggaaacaa cagtcaccga
    20821 agaactgaca gctgctttgg aagaaatcgt tgaggagcct aaagcagaaa aagtcaaagg
    20881 cgagctagtg acactgttag atgacagtaa ttttttgata ggtgaaagac attatcggct
    20941 tgtgtccgat tatcgtgaag gatttaatgc agaaaagctt ggagaacgtt acagtgatgt
    21001 actagcccga tatgattata tcgtaggaga ttggggttat gaacaactac gcctaaaagg
    21061 atttttccaa gcggataatc gacgggcgca tccagatcag cgaatcgatt ccttggaaga
    21121 ctatctttac gaatattgca attttggttg tgcgtatttt gtcatcgaga gaatcggcgg
    21181 taaaaaagaa aagacaactc agcgccgaaa aaagaaaaaa aaccataatc gtgcgtatat
    21241 cgatgagaaa aaaggaccag tcgcatctaa tcgcaaaaaa ccggttatta aaaatcgaaa
    21301 aacagagtca gcaaaaacct ctgaaaaagt aaaaaaacaa gaacaaccta aacaagtaga
    21361 aaaaacaaaa acagcattta cgatcagaaa aagggaagag tagtggatga atggactata
    21421 aaggatattt aattgattta gacggaacga tttatcgtgg gacacaaccc ataccggccg
    21481 gtaaacgatt tgtggaagaa ttgcaaaaaa gaaaattacc tttcttgttt gttaccaata
    21541 acacgactaa gtcaccagaa acagtagcta atcgtctagc agatgagttt gacattcatg
    21601 tagctccaga gacagtctac acagctactt tggcaacaat cgattttatg aaagcagatg
    21661 gaaaagggaa aaaagtctat gttatcggag aagcaggatt gattgacttg attttagctg
    21721 ccggatttac atgggaagaa gaggctcctg actatgtagt ggtaggctta gataattatc
    21781 tgacctatga aaaagtagtt aaagcaacac ttgctattca aaaaggtgct actttcattg
    21841 gaacgaaccc agataagaat atacctactg aacgcggcct tcttcctgga gcagggtcag
    21901 tcatctcttt tgtagagaca gcaacccaaa cgccgccgat ctacatcgga aaaccagaag
    21961 ccatcattat ggataaagcc gttgaagtat tagggctgca aaaagaagaa gtaatcatgg
    22021 ttggagataa ctatgaaaca gatatccaag ctgggatacg caataatata gatactttgc
    22081 tcgttttatc aggttttaca aaagaagaag atgtacctgg gttgcctatc ccagctacct
    22141 atgtgaaaca atcactggat gaatggagct tctaatatga aaaattcgag atggcagtgg
    22201 atggaatatg ctggattgtt cagcctattt ctgacactga tcagtctggc agttggtgtg
    22261 acgatcaact ttcgaccact atacgtattt gatatcggac atttgcaaat tttggattat
    22321 acttctttag atcaagagac gttactgaaa aattttgatc acctaatgaa ttatctcaat
    22381 aatcccttca aaaccatcct cagcttgcca gatttcccag tttctgctag cggagcccat
    22441 cacttttatg aagtgaaaat tttgttttta gtggattatg ctgtgttttt catcactttg
    22501 atcccgagta ttttgtttat caaatatttg caaaagaatg atcggctttg gcggttgatt
    22561 cgtccgtttc aaatcggcat gttactgcca gttgttttcg gttttttcat gatgatagga
    22621 tttgatcgct tttttatcct ttttcatgaa acgtttttca acaacgatga ctggttattc
    22681 gatcccgtta ctgatccaat catcaatgta cttccagaag aatttttcat gcatagcttt
    22741 atcttatttt ttgtattatt agaattattt tttgctgttt ttcttttctt aggaaaaaat
    22801 agcttgaaac aaacaaaaaa gaaagaactc gtctaaatag acgttttctt tctttttttg
    22861 ttgaattatt gagtaaggcg acttcttttt tctggttctt tgtgtgctaa tcgactggca
    22921 gcagcagcgg cgatcgctcc gacgatatca tctaagaagg tatggacatg aacgccatcg
    22981 tggctgttta gttcttttaa aataccaggt ttcaccttgt cgatataacc ataattcgta
    23041 aagccgatcg taccgtaaac attcacgatt gacaaggcta ggatctcatc gataccgtaa
    23101 aggccttcgt catcttcgat aatatgttgc aacggactca tcaatttttt cttttctgct
    23161 aatatatcta attggattcc agtgataatg gcgttatgta cttcacgctt tgttagcacg
    23221 ctgttgacac tttcagtaca atccgccaaa gtcaaatcag aaatatagtc tttttgtaaa
    23281 aacatgacaa gttgtgcgat atcttctaca gttacgccac gttccttcaa tagttctctt
    23341 gccttttctt ctaaagtctc agttttgacg accatattat tgctccccct ttattttttc
    23401 tttctctatt tcatgttaac ggaacatgaa aaaaatgcaa gaaactgctt tcgggtttaa
    23461 cttgaaatgc aaagagatat aaaaaatcaa ctaggacgaa gatttttatc gtataagctt
    23521 gtgctgtgac caggctgagt tcgttccttt ggattaatat agtgtaaagc gttgttcact
    23581 gcagtaggtg cttcaccgag cccagtagca atcagtttca cttttccttc ataggtacag
    23641 atgtcaccag ctgcataaac gcctggaata gaagtagaca tatctgattt tacagtaatg
    23701 gcatttctgg tactatcaag tccccaagaa gaaagatgtt ctaaattaga agagaatcca
    23761 taattgacga tcagagaatc aatcatcaaa tcaatcgtct cgtcactttt cacttttttt
    23821 aaccggatat ctgtcagttc cccattattg cctgataatc catcgatgag atagggagta
    23881 agcaagttga cagatgaaga tttcaagcgt gatacactat gttcgtgcgc acgaaattcc
    23941 gggcgacgat gaatcaagta aacttcgctt gcaataggct ctaacatcaa cgtccaatcg
    24001 atagcagaat caccacctcc agcaatagcg actttttttc cagcatactt catcaaatca
    24061 ttcacgaaat aatccaatcc gtggttttca aatgattcag cattatctaa attcaatttt
    24121 cttggctgga atgaaccatt tcctaaagct aaaatgactg ctttggaata atggatgcct
    24181 ttatttgtag taatctcgat aacttcgtct tcacgagtaa gcgagagaac ttcttctttt
    24241 aaatggaatg tatggttaaa agtagttaat tgtttttcta attgatcgat taattcactt
    24301 gcttttattg ctggataccc aggaatatcg taaatatatt tttcgggata caaagttgct
    24361 aattgaccac caagttgagg tagactgtca atgatttttg ttttagcttg gcgcattcct
    24421 gcgtaaaaag cggcaaacat ccctacagga cctgcaccga tgattgtaat gtcatagatt
    24481 tccataaaaa ttcctccctt ttcacactat atcagaattt aattgagaat gcttctcaaa
    24541 aatgtttgaa ttcgaaatcg aaacggtaag ttatagaatg tatctagtga tgaaaaataa
    24601 aagaaaacaa atctagtatt tttacgagag gttaggtatt atgttattat tattatgaag
    24661 tatgcaaata ttaggaggaa acaatatggc attcccacaa ctagatttaa aaagtgaaaa
    24721 aggaccaaaa gctgttatca aaacaaaccg tggtgacatt actgttcaat tattccctga
    24781 actagcaccg aagacggttc aaaatttcat tgaactttcc aaaaaaggtt attatgatgg
    24841 cgtgattttt caccgcgtta ttcctgattt catgatccaa ggcggcgatc caactggaac
    24901 aggaatgggt ggcgaaagta tttatggcga aagttttgaa gatgaattca gtcgtgaact
    24961 gttcaacctt cgcggagctt tatctatggc aaattcaggt ccaaacacaa acggaagcca
    25021 attctttatt gtaaataaca caaatgtgcc agctaatatg cttggacaac tagaaggtgc
    25081 tggattccca tcagaaatca ttgaagcgta taaaggtggc ggaactcctt ggctagattt
    25141 ccgacataca gtctttggac atgtactaga agggatggac acagtagatg aaatcgcaaa
    25201 tgtacaacgt ggtccacaag accgacctgt acatgatgta gtgattgaaa caattgaaat
    25261 ttcagaatag tttggtatgg tacggagtaa ttatgaaaaa ggacgaaacc tagtaaggtc
    25321 tcgtcctttt attttgaata aatgatttaa tcgattgtct ctaaagcata cttgattcga
    25381 ttaagtcctt ccagtaaagt ttcttctgga caagcaatat tcaagcgcat atgcccatga
    25441 ccggaaggac caaacgtgat gccaggattt aatacgactt ttcctttccg aacaagagtg
    25501 ttctccaatt cttggtcgtc tgaaatatag gcggtaaaat ctaaccacat taagtaagtt
    25561 ccttcagggt tcattattct cactttcgga agatactgtt caaaaaactg attaactgtt
    25621 tgaacatttt tttctagata aggaatcagt tggcttaacc attctccacc cgtctcataa
    25681 gccgcttggg ttccaatcag tccaaaagtg ttgatttcgt gctgttggtt catcactaaa
    25741 tgtttttcaa aatcagcttt catctctgga tttttgataa ataccattga atttttgatt
    25801 gcagctagat tgaaagtctt ggttgcggaa gtaaaagcaa ctagtagatc atctaaatta
    25861 ggatcaatcg ttagcatgga agtaaatgta tgatcgaata aagttaaatc ttgatggatc
    25921 tcgtcactaa acagaatcac ttgatgtttt aaacacaatt cgcttaactg tttcaattct
    25981 tctttgctcc aaacacgccc tccaggattg tgaggattgc ataaaatcat tgcttttaca
    26041 ttgttttctt cgattttttt ctccatgtca gacaagtcca tgatgaaatg attatttttt
    26101 tctagcaaag agcttcgaac gatgtgacgt tgatttgttt ctataataga ggaaaatgga
    26161 ggataaactg ggtcatgaat caaaatagaa tcgcctggtt ttgtaaaagt ttgtactgct
    26221 gcagctaaac tggttaacac accacttgta aaaacgatat tttctttcgt caactccaca
    26281 gcatggtgtt ggcgttccca atgaatgata gcctgatata gtttatcaga cagagtggca
    26341 taaccaaaga ttccatgttt gatgtattca ctaaacgctt tagctacccc accgggagac
    26401 aagaagtcca tatctgctac ccacaaagga agtaagtcat ccatttgata tgtctcagca
    26461 attgcgtccc attttacact ttctgtatct tttcttgaaa tctgtttatc aaattcgatc
    26521 atcttaagac ctccagtcat tttcgtcgct tctttttttg agctatttac agtataatga
    26581 taaatgcgaa gagagggaag tagatgactt ataaaattgg acaaataatt gaaggaaaag
    26641 tgacagggat acagccttat ggcgcattcg tttctcttga taacgagacg caaggactta
    26701 ttcatgtttc agaagtacaa tccggttata cgaaaaatat ccattcattg ttaaaagtcg
    26761 gacagccagt aacagttcag atcatcgaca ttgatgaata ttccaaaaaa atcagtttat
    26821 ctttaagaac gttagaaaaa acaaatccag ctgttccata tcgcagaaaa aggtatttca
    26881 cgaataaaaa tcgtaagatc ggatttacca ctattgcgga gcagttacct atttggatag
    26941 aagaagcatt atctgaacta gaacggaaag aaaataagaa ataaaaagtg tattttttct
    27001 gaaaatgtgc tagaattttt atgtaagcca tagaacaagt tgatcgaggt ggaaattttg
    27061 gcaagcaaaa gagttgctgg tacaattatg ttgcatttgg aggatggtac taaaaaattt
    27121 ttggtccatt caattgatga caagttggaa tttgcgttag cggaactttc tgaaggaaag
    27181 actggtttag cgaatatact taatttctta aaagaaatag ttcatatcga tgttagcaag
    27241 attagtttga tggaattgac aaatacacat attaaagagg aaaatgttcc gttgtttgtc
    27301 tttgaaactg aacaaaagaa tcaaagagaa gaattaggcg aaggatatat ttgggaagaa
    27361 ccaggtcaaa tgcgctctgt tcttggaact tacgaagtag agggagtgcc tttcttctaa
    27421 aatacagatt aagcgaacaa agaagtttga atttttttca aacttctttt tttattgttt
    27481 ggtccaaaaa atacaatgaa atgttctgct aaagatagtt ttttagtgca ttttattttc
    27541 aaa
//
LOCUS       DAZBSA010000030        27514 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000030 DAZBSA010000000
VERSION     DAZBSA010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.030"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..645
                     /locus_tag="ACHGMT_001163"
     CDS             <1..645
                     /locus_tag="ACHGMT_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFN6834922.1"
                     /translation="KKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSDS
                     STKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYFY
                     KIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAAK
                     IAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            729..1673
                     /locus_tag="ACHGMT_001164"
     CDS             729..1673
                     /locus_tag="ACHGMT_001164"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFN6834923.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1808..3259
                     /locus_tag="ACHGMT_001165"
     CDS             1808..3259
                     /locus_tag="ACHGMT_001165"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFN6834924.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3280..4116
                     /locus_tag="ACHGMT_001166"
     CDS             3280..4116
                     /locus_tag="ACHGMT_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFN6834925.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4116..4775
                     /locus_tag="ACHGMT_001167"
     CDS             4116..4775
                     /locus_tag="ACHGMT_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFN6834926.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            4901..5590
                     /locus_tag="ACHGMT_001168"
     CDS             4901..5590
                     /locus_tag="ACHGMT_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834927.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5596..6552
                     /locus_tag="ACHGMT_001169"
     CDS             5596..6552
                     /locus_tag="ACHGMT_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFN6834928.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6596..7108
                     /locus_tag="ACHGMT_001170"
     CDS             6596..7108
                     /locus_tag="ACHGMT_001170"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6834929.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7364..7912)
                     /locus_tag="ACHGMT_001171"
     CDS             complement(7364..7912)
                     /locus_tag="ACHGMT_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFN6834930.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8001..9182)
                     /locus_tag="ACHGMT_001172"
     CDS             complement(8001..9182)
                     /locus_tag="ACHGMT_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFN6834931.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(9418..10926)
                     /locus_tag="ACHGMT_001173"
     CDS             complement(9418..10926)
                     /locus_tag="ACHGMT_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFN6834932.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            11085..11276
                     /locus_tag="ACHGMT_001174"
     CDS             11085..11276
                     /locus_tag="ACHGMT_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834933.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            11479..12396
                     /locus_tag="ACHGMT_001175"
     CDS             11479..12396
                     /locus_tag="ACHGMT_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFN6834934.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            12389..13462
                     /locus_tag="ACHGMT_001176"
     CDS             12389..13462
                     /locus_tag="ACHGMT_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834935.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            13610..14128
                     /locus_tag="ACHGMT_001177"
     CDS             13610..14128
                     /locus_tag="ACHGMT_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834936.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            14109..14501
                     /locus_tag="ACHGMT_001178"
     CDS             14109..14501
                     /locus_tag="ACHGMT_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFN6834937.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            14577..15512
                     /locus_tag="ACHGMT_001179"
     CDS             14577..15512
                     /locus_tag="ACHGMT_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFN6834938.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            15645..16424
                     /locus_tag="ACHGMT_001180"
     CDS             15645..16424
                     /locus_tag="ACHGMT_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFN6834939.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            16417..16734
                     /locus_tag="ACHGMT_001181"
     CDS             16417..16734
                     /locus_tag="ACHGMT_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFN6834940.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            16978..18588
                     /locus_tag="ACHGMT_001182"
     CDS             16978..18588
                     /locus_tag="ACHGMT_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFN6834941.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            18745..19593
                     /locus_tag="ACHGMT_001183"
     CDS             18745..19593
                     /locus_tag="ACHGMT_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFN6834942.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            19824..20303
                     /locus_tag="ACHGMT_001184"
     CDS             19824..20303
                     /locus_tag="ACHGMT_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFN6834943.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            20336..22564
                     /locus_tag="ACHGMT_001185"
     CDS             20336..22564
                     /locus_tag="ACHGMT_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN6834944.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            22693..24336
                     /locus_tag="ACHGMT_001186"
     CDS             22693..24336
                     /locus_tag="ACHGMT_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFN6834945.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(24409..24954)
                     /locus_tag="ACHGMT_001187"
     CDS             complement(24409..24954)
                     /locus_tag="ACHGMT_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFN6834946.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(24977..25462)
                     /locus_tag="ACHGMT_001188"
     CDS             complement(24977..25462)
                     /locus_tag="ACHGMT_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFN6834947.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(25452..26264)
                     /locus_tag="ACHGMT_001189"
     CDS             complement(25452..26264)
                     /locus_tag="ACHGMT_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFN6834948.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(26403..>27514)
                     /locus_tag="ACHGMT_001190"
     CDS             complement(26403..>27514)
                     /locus_tag="ACHGMT_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFN6834949.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         9009 a         5016 c         5512 g         7977 t
ORIGIN      
        1 aaaaaaactg ttatttatac tagtttattt gcagctttgt ttttagctgc ttgtcaatca
       61 aataccaatc aaggcacatc tgggaatacg tcttccagta gcgaacagcg ctcttcacag
      121 gtatcggact cctcaacaaa agcaagcaca tcttcgtcta gccagacaaa aagttctagt
      181 gaagcaagta ctcagtcaga tatcaaagtg tctgcagctc gtgcgatcga attgttccaa
      241 gataaatacc cagatactgc aattactagt ttggaattag atagtgattg gggatcttac
      301 ttctacaaga tagaaggtgt cgatgatcaa aaagaatacg aagcaagaat tgatgctcaa
      361 aatgaaaaaa tggagacaga gaatccagaa actctagatc gagatgaaca aaatggtcaa
      421 aagaaaaaag aagatggtct ggatgttgat aaattgatca cgattgagga agcagcaaaa
      481 atcgccgtcg acaaagtagg aagcggtacg gcaacagatt gggatttgga taaagattta
      541 ggtactactt attgggaagt caaagtaaaa gacggcaatc aaagtacatc agtcaagatc
      601 aatagccaaa caggcgaagt actgtcaact gaacaagatg attaagaatt tcttcggaaa
      661 acaagagttg atttttcttt tagtcatgat acactagaca aaataacttg tttgaaggag
      721 cgatttactt gagtttagaa acgacctttg ccaatcatat ctttgccaat cctttgatga
      781 atgcatctgg tgtccactgc atgactaccc aggaattaga tgaactggct cattcagaag
      841 caggagcatt tatcactaag agctgtacga tcaatgaacg aaaaggaaat cccgaaccac
      901 gctattttga tgttccttta gggagtatca attctatggg acttccaaac ttaggttttt
      961 cttattatct agagtatgct cttgcgtatg aaaaagtgca ggaaaaccaa aatcaaccgt
     1021 tatttttttc cattgccggg atgagtgtcc aggaaaactt ggaaatgctt gagaaaatag
     1081 aaaaaagtgg gtttaatggg atcactgaat taaatttatc ttgtcccaat gtgcctggaa
     1141 aaccacaact agcttacgat tttgaggcca cttatgaaac actgaaagaa gtattcagca
     1201 tcttttctaa gccgctagga atcaaattgc ctccttattt tgattttgct cattttgatc
     1261 agatggctga tatcttgaat cagtttccat tgacctatgt caatgctatc aacagtgtgg
     1321 gcaatggttt gtatatcgat acagaacaag aagcagtagt catcaaacca aaagaagggt
     1381 tcggcggcat tggcggagag tatatcaagc cgactgcttt ggcgaatgtt cgcgcttttt
     1441 atacacgttt gaaaccagaa atccaaatca ttggtacagg tgggattcgg acaggacaag
     1501 atgcatttga acatctgcta tgtggcgcaa gcatgttaca gattggtacc gagcttcata
     1561 aagaagggcc ggaaattttt tctagaatca taaaagaatt aacgcagatt atgtcagaaa
     1621 aaggatatac gtcaattgac gaatttaaag gcaaattacg caccatttca tagcaaagag
     1681 acgaaggtca tgattgatct tcgtcttttt ttataaaaaa cactcgtctg ttcattcctg
     1741 tgcaagcgta ctcattgtat gttagacgaa cttgtgggaa aataacagag gcaaaaagga
     1801 ggataatatg aatgatgcaa cagaattagc aaaagagctt aaagaaaaag aatttagttt
     1861 tcaagaacta gttacggata tacagaaaaa aataaagaag caaaatcatg aattgaatgc
     1921 gtttgtcgcg cttgaaccat tcgagagtaa ttcccacgag ctattgattc cccagtcaga
     1981 aaaagggcca ttggctggta tcccatttcc tttaaaaatg ctcggtcaag aaaaaaaggg
     2041 atggctagct acttcaggat ctcgtttatt tgaaacacac cgagcttctc atacatctaa
     2101 ttatgtgcgg caagcagaag cgataggtct agttcctttt ggacaaacca atgcgccaga
     2161 gtttggtttc aaaaatatta cagacccagt catctatggc ccagcaagaa acccttggaa
     2221 ccttgaatgt acaccgggcg gatcaagtgg aggagctgct gctgcagttt catcaggaat
     2281 cgttccttta gctggtgcta gtgatggcgg tggttctatt agaatacccg cttcgttttg
     2341 cggattgatt gggctgaaac cttcgcgagg gacgatgcca gttggtccat atgcttggag
     2401 aggatggcaa ggagctgcta ttgatttcgg cttgacagtt tctatgagag acacggaggc
     2461 tttattttat gggatgagaa gtatccattc cggtgcgcca tatcaagtat cgccagttga
     2521 gtggcaaaca catccaagaa aaaaacggtt gaagatagct ttttgtgttg attcaccgat
     2581 ccactcacag attagtgaag aggcagtact tgctgtaaaa aaagctgtag atttccttgg
     2641 aaaagaaggg catgatttga cagagattcc ttacccacta gatggtcgta aactgatcac
     2701 gtcttattat cagatgaatg gcgcagaaac agcagcaatg atccgctcga ttgaaacagg
     2761 catccagcga gctgtcagaa aagaagaact tgaacccatt tcttggactt tgttccaata
     2821 tggtcagaag ctctctgccg ctacttacat cgattctctg catgtatggg atgaagcagc
     2881 aattgtcatg gaaaacctac atcaagaata cgatctattc ctttcaccaa cgactgcatt
     2941 taccgcccct aaaatcagcg aagatttgca aagtgatgcg ataagagcac agatggaaca
     3001 catcagtgat tgttctgaag tagaaggttt agacgttgtt tcttccatgt ttgaggagag
     3061 cttgaagatc acaccttata cacagttggc taatctgact ggacaaccag caatcagctt
     3121 gcctactcat ttgacaaaag aaaacatgcc tctaggaatc caatttatgg ctgccagagg
     3181 aagagaagat ttgctatttc aaatcggcta tctatttgaa gcaaatcatc agttccattt
     3241 accaaatcca tggaaatagc agaaacaagg agaagaagaa tgaagaaaaa aagattgttt
     3301 gctctaataa ctagtgcctt ttttatttta agcgcttgcg gtacagctgc cgctaatcag
     3361 gaagataaca gctggagtaa tgtagaaaaa aagaaagaat tagtagtagc gacttctggc
     3421 acactgtttc ccgcatctta ttataatgat caaaatgaat tagttggtta tgatgtagat
     3481 ttggcaaaag aggtggctaa acggttaaat gtcaaaatct cctttaaaga atacaacgtg
     3541 gatggacaaa tcacttctgt ttcaaaaaga gaatcagatt ttgctgcgaa cgattttggt
     3601 atcacaaaag aaagagaaaa aaaattctcc ctttcagaga ctttcaaata ttcatttgac
     3661 agtatgatcg tacgaaaaaa agatgattca ggaatccact cacttgaaga tcttaaaggg
     3721 aaaaaagcag ctggcgaacc caatactagc tatatgcgtt tagcggaaaa acacggagct
     3781 aaattagtca catatgacaa tgccacgaat gatcagtatt taagcgatgt agcaaatggt
     3841 cgaacagatg ttattctaaa tgattattat ctgcagaaaa tggccgtatc tgctttacca
     3901 gatatccctg taaaaatact ggagaacgtt tatttcaatg aaaacgaaag tggtttttta
     3961 ttgaaagaag gaaatgatgc tctgaaaaag aaaatcgatg gcgtattaaa agagatgaaa
     4021 gaagatggga cgacaaagaa aatatctgaa aaatattttc aaactgatgt ttcagtcaaa
     4081 ccgaaagaaa cgatggctga gtcggtgagt gagtgatgta ctttcctcta attgattttt
     4141 ccctcatgtg gacttcactt cctttcgtac tgcaaggctt ggtatacaca ttagggattg
     4201 gcttcgtgtc gtttctttta ggaaatctgg tagggttgct tttgacagtt ttaggtcttt
     4261 tggattggct gcctctgaat gtatttattc gtttttattt atcgttcttt agaggcattc
     4321 ccgcactagt cctgctgttt ctgttatatt ttgggttacc ttatcaatta agtgccttaa
     4381 cggcttcagt catctgcttt acgattacca gcagtgcatt tattggtgaa atctatcgag
     4441 gatcactagc aggcgtttct tcaggtcagt gggacgctgg tttagcttct ggtttttctt
     4501 tctttggggt attgaaatat atcgttttgc cgcaagcttt tcgaatctca gtaccggcac
     4561 tcagtaacgt agctatggat ctagtgaaag gtacttcgtt agcagcaaca atcacagtgc
     4621 cagaaatatt tcaaaaagca aagatcatag gcggtcggac attcgattat ttttctctct
     4681 atattttagt tgcactgatt tattgggtca tttgtatcgg gatcgaaaaa gtgcaacagt
     4741 atttagaaaa acgttctatg catcagtcaa tctgaaaaat agaaatggtg ctagcgtggc
     4801 tggcaccatt tctatttttt gtttcttgta tattctgcct agtattttaa aaccatttca
     4861 tgtaaaatgt aaatcgaaaa tatgttcgga ggtagaaaac atgaagaccc cgctaaccat
     4921 cgaaatagaa tcctcgttat accaatattg catcgagcaa ggcggcttgg ttgtagaaga
     4981 agtcacgatg cctgatgacc aaggaattgt agatacgctt agctgcttga caaaatttga
     5041 tggaagccga gaatggcgat gctatgagct gaaagtgaca aaagcagatt ttcgctctac
     5101 agccaaactg tcatttgttg gacattataa ttattttgtt ttaccaagta aactatatga
     5161 agaagtaaaa actgaaatcc ctacaggaat aggtgtttta gtgtatcgtc cgtacacggt
     5221 tgaagaggag atgcctgcct ctggtacatt tctgattgcg aaaaaagcag caaagaaaga
     5281 gcttgctgta ccggaagaag ctttgacaaa ccgcttcatg gcttctttat tcagggaagt
     5341 aagaaaagca aagcaaatgg agcgaggcgt aaaatatttt ccaacagatc aattgtacaa
     5401 agaattgaaa agacgttcag aaaatcaaga tccattttct aacgaacgat attatgaacg
     5461 gtttttagag gaaatagcta gtcaggcaat caatgagttg caagaagaag ttatcgcgct
     5521 tcagcatgat tatgaatatt tgaggcaaca acagcagatc cgtcggttgc ctacagagcc
     5581 attggagtga aaaatatgta ctatccttat ttaagaggaa aacaatttga cctattagca
     5641 ttaaaagaag cactgtcgcg aggattgctc tccaataaaa tccaaccagt gatcgaacca
     5701 gtacgtgatt ccgctacgct taaaaatgtg atcgaattat ttcaaaaaaa gcaccatcga
     5761 atcgctgtga tccaaaatcc ccaagtagga caattcaaat tattcgatca gcatgtgcac
     5821 agttggacag tcaaagaaaa tagctctgtc gtccctgcac agatcttaac gccagaaaat
     5881 ctcagtgaag tactggattc acctccagcg tttcttgttt tcgatggaca gcactaccca
     5941 aaagatacag aggtgtggaa acagttggca ggagttgatt cgaaattttt gataccagat
     6001 actagtcgct ttcgcatctg gctgcccgaa aacaaaattg tgatccgtga ctcttttcaa
     6061 acgagaaagc atgttgaaag ttatgcagat aaaacagatg acttttttag tgatgactat
     6121 ctttttttcc acgcagacgg atatattgga ttttctgatt tcacgatcga aggtagccgt
     6181 tatttcgata aagggttccc tagccgagcg cttgctatcc atctaacgta tattgatgct
     6241 tacggcaata tacgcgtgaa acattttgtc tctgacagta acgactctgc taaagaccaa
     6301 gcattgaagt ttcttgaagc aggagataaa atgaaaaaat ggatcatgcg tcaccatcat
     6361 caactcttga ttacttcagg aatgattgaa ttattggctc tttaccagca acaaaaattc
     6421 cctggactgg gagtgttgaa aaagtggtca cttatgcatc atttggaact tattagtcac
     6481 ctgctagatc atccaaaaga ttggcttaaa tattattcaa aagttcagga actatatgaa
     6541 gtgatccaat gattagataa tgtaaatgaa aaaggaatag aaaagaggaa actggatgta
     6601 tattcggcaa acaaggaaag aagaagtaga agaagtggta gaaatcatta atgccgcagc
     6661 ggatttcatt gcttcgcaag gctctcctca atggcaaaac gggcagaaac caacaaaaga
     6721 aacgattttg aacgatattg aaagaaaaga aagctatgta ttaatagtgg aagatcaaat
     6781 tgctggaaca gctgcattag tctcaagcgt ggatcccgtt tacacaaaaa tagatggtag
     6841 ttggaaagaa tcaactggtc cttatttatc gattcatcgt gtcgctttag cagaaagtac
     6901 tcgaggaaaa aaactagcaa aaccgttctt agaacatttg ttgacgattg cagccagcaa
     6961 aaatatccaa gatatccgta tcgatactta tccagctaat gaaccgatgg aaaagaccat
     7021 ctattctgct ggattcactt atcaagggat gattgaattt ccttttgaac acggcgaaag
     7081 aaaagcatat caaataatca tcaattgact ataaaatcat ctgagcaaag ataaagacat
     7141 taacactact gaaaaaaaca atcatatgat agccaagagg ttgtaacaga agtggccagc
     7201 ttcaaaaaac aagaaagaat ttccgaaaat gcttctcaaa tttttgtgga attttagcgt
     7261 attttacgaa atagctgctt tctgaaactc ccttcatcga gaggtcgtga catagctttt
     7321 gtcacgacct ctcggcttat cctacgagta ttattcgata catttatatc aataatcctt
     7381 tttttatttt atctgcagct tcttggaacg ctccatcgtt tgacacgact aaaagtgagg
     7441 ggatagcttt caaacgtttc aactgtgcta aatcttctac ataaaccgcc agtctggctg
     7501 aaatctgttc agatgaatct ccacgttttt tcaggcgcga ttgcaatgtt tctttagaga
     7561 tcgtaataaa gataggacgg actaccgtcc cgaaatactc atataaatgc caaaaaccat
     7621 ttgctgtcaa aacggcgtaa caatttccta ctgtcagttt ctttttaatt tctgctttac
     7681 tggaaccata tttatgttga gcatattggt cccactctgc aaactcattt cccttgatca
     7741 tctgattgaa tgtattgtca gaaacaaaat aataatcctc ttgatctttt tccccaggtc
     7801 ttggtgatcg attcgtgtaa gtaatgatct tttgttccgg ctgaaaaata gcttctgcga
     7861 gtgaagtttt tccagaacca gaaggaccga taaatacata gcaataattc attgtcattt
     7921 ctcctttgat gattgaacaa gtgaaaaagc tttggaaaat atttccaaag cttttaaaag
     7981 taactttgct tttttgtata ttataccagc aagccagtca caatagcact taaaaagctg
     8041 actaaagtcg ctgttagcaa aattttcaat ccgtgttttg cgacaagatt tcctttttct
     8101 tcatttaatc ccttcatcgc accagaaatg atcccgatag aagagaagtt ggcaaatgaa
     8161 acgaggaaga cagatataat tgctgttgta cgctcagtaa aatggaaatt accatttgct
     8221 aattctgtca tagcaacaaa ttcatttgta accagtttag tcgccatgat actaccggca
     8281 tccactgctt ctttccatgg gattccgctg atgaaggcaa gaggggcaaa gatgtaacct
     8341 aggatctctt ggaaagtgat gccgaatatc ccgtggaaga tagcattgat catggcaatc
     8401 aaagcaacaa aaccgataag catagcagca accgtgatcg ccacatggaa accatcaaga
     8461 atgtattctc ccaacatttg gaaaaatgtc tgttctttgt tttcttcaat gatcagctca
     8521 tcttcttttt cttctaatac atagggattt acaattgaag ccagaatata accgccaaat
     8581 aagttcaaaa caagggcagt gatcacatat ttagaatcga tcagtgccat ataagatccg
     8641 acgatcgaca tagaaactgt agacatagcc gatacactca tggtgaacaa acgtttctca
     8701 gataggaagg gtaattcctt ttttatggaa ataaaaactt ccgattgtcc taaaatagca
     8761 gaagctactc cattataaga ttccaatttg cccatcccgt tgattttact taatcctaga
     8821 ccaacagctt tcataaataa tggaagaatc ttgatatagc gtagaatacc gataatagca
     8881 gaaataaata caattggcat caatacggaa agaaagaaag gtgcacttcc tttatttgct
     8941 acaccggcaa aaacaaagtc aacaccttgc ccagcaaaag caagaagatg atcaaacatt
     9001 tttgccaata cagaaacaac cgctgtacca aaagttgtac gtaataatac gaaaccaaga
     9061 acaagctgta aaacgaacat cacaattagt ggtttcaatt tgattgcttt tttgttactg
     9121 ctgatcaaaa aagcaagccc taatgtgaca aataaaccaa taatacctaa taaataactc
     9181 aaataatcac tcctattcca aattccgttt tattttacct gttttgcaaa taaaatacta
     9241 atctttagtg aaattttcat tactttttta gagtgaaacg cattcatttt ccattagaaa
     9301 ataaagcgaa caaaataaaa ggtttaaaaa gtgtaacgtc ttagtaaagt ctataaaaaa
     9361 acgaaactat cgattaagct aatagtttcg ttttcaacat ttataagtaa actttttcta
     9421 ttggtaatat agattttcgg aagggtagac aggtgcgcag gtaatatcga ttcctagttt
     9481 tttcaatgtt tgttcatcat ttttattgac catcacagta gagtgggctt gtgctccctc
     9541 aagttcagct agtttatcat aagcaacttg agcgattgga ttcgttactg cactgattga
     9601 taaagcgatc aagacttcat tgatgcttag tgcatccaaa gaggaatgaa gtgtctttga
     9661 tttcaatcct tgtattgttt caagaactaa tggtgacaat aaaggaatgt catctgcgat
     9721 atgggctaaa tgttttactg catttaagat aactgccgct gacgcatcca tcaatgaact
     9781 tgtacgcccg gtaatgatga cttcatctgg taattcgatt gcaatcacag cagaaggatc
     9841 ttttgtagga ctttgcgctt gaatcttatc tgcatagtca tgggcaggga ctaccacgtt
     9901 tcggtcttca ggacgtaaac cgacttcttc cataatcagt ttgattcgat taactgcttc
     9961 ttcgtcgatc aatccttttt tgaagtcgca ttctgtttgg aaataacgac ggatgatttc
    10021 ttgtttagag gcttcttgga caacggtatc atccacgatc ccaaatccta cacgattcac
    10081 acccatgtca gtaggagatt gataaacaga ttctttacca gtaatttttt caataatacg
    10141 tttgattaca ggaaatgtct caacatcccg attatagttc acagctactt tattgtaggc
    10201 atcaaagtgg aaggaatcga tcatattgac atcttttaga tcgactgtgg ctgcttcata
    10261 agcaatgttc agcggatgtt tcaacggaac attccatact gggaaagttt cgaacttgga
    10321 gtaaccagct gcatgtcctt tttggctttc atgatacagt tggttcaagg aagtagcgag
    10381 ctttccactt cctgggccag gagctgtcac tacaacgatt ggtttagttg tttcaatata
    10441 ctcatttttt ccaaaaccat cttctgagac gattttttct acgttgactg gatagtcttc
    10501 gatttctgag tgtttataga ccttgatgtt tcgtctttct agtttattga taaagacttt
    10561 cgtggcaggc tgtccactat agcgagtgat aacgacactg ttgattaata agccataacc
    10621 acgtaattca tcgattaacc gcaagatatc catatcataa gtaatcccat aatcaccacg
    10681 gatcttatta cgttcaatat ctccagcata gacgcaaatc agtatttcag cttgatcttt
    10741 caatttttgc agcaatttga ttttcgcatc ttcttcaaat cctggaagta cacgtttcgc
    10801 atgcttgtcg tcaactagct tcccaccaaa ttctaaatat aatttgtcat agtggttcac
    10861 tcgttccaaa atatacgctg actgctcttc aatatatttt tctgaatcaa aaccaatttt
    10921 tttcactagg ttccctcctt aagtactaaa gcattttttc atttttttga ggattcgtca
    10981 actggaagag cgaaattcct caaaaaaaca aagaaaatgg tttaaaccaa cttgtctaga
    11041 tagttattca ttggtatgat agtttttgat aaggaaggtg ctacatgaaa aaaagacaga
    11101 aaaagaaaaa cgcatataaa cactatatcc gttcgatttt taccggttat gaaagaatgc
    11161 ttgaagatcc cgaattggaa caattgacgt ttacttatct aaacgaagaa acgcagctaa
    11221 ctcgtgatga gcatcagcgc attcatttca caacaagaga tctgcctagt aaataaaaaa
    11281 taagataaag gtcatcggat tcgattctgt cctatcagat ggaaagacta gttgatcgaa
    11341 aaaaaggctg taccaaattt tggtgcagcc tgttttatgg agcattgccc gctctttttt
    11401 ataacaattt tttacagaaa atggtactat tgaagtatta ataagtgaaa aacagatgag
    11461 taagaggagg caagtagtat gaaagagcca gatacaaatg caaattatga gccagacgaa
    11521 ctagaggaga cgaatgcgga tcaggataca ggacaagaat cgtcttcttt ttacgatatc
    11581 aaagaaaaag caagcatgaa tctttcccaa ttattagaag acttacaggc ttttgaaaaa
    11641 gctgtccgcg aagaagatat ccctgagatc taccgtttat acaatggccg attgaatgaa
    11701 gaattaaaaa gaagctcaaa tgaaaatcac gagatcgatc aattgctgat gcgtaagatc
    11761 catgatcgtt ttttacagga atttcctttt atggaacaag tagaaaacat ctcgccaacg
    11821 atgagctatt acaaaatcgg cacttattac catgatcggc ccacggtcgg tattgatgca
    11881 agtctgccag aaatttttgt tttgcctaag atcgatgaag agtgggagaa atattcccaa
    11941 ccggagtcaa cggactatga caaaaaaatc aatgagctag acgcaaaggt gatcactgct
    12001 cagacagaaa ttgaacgttt agatgaacaa ataaaagaaa tcaatcgaca aatgactgat
    12061 ttgaacgaca ataaaggatt cttgaatcgt aaaaaaatcg aggaagaaat tcaagaatta
    12121 gagaagaaga agcaagtact atctaatgaa aaattaggct ggcttcctta tattgagacg
    12181 cctgaaacaa tccagcagca aaaagaagca ttgaagcaag aagcaagagc agaccagctt
    12241 cgtgcagcca tcgtagaaaa agaacaaagg caaatcagac gctatttcgg tagcaaagag
    12301 ggattcggtc aagcaatcca tgaattcttg atgaattatc ttggcaatga gaatcctaat
    12361 aatcaatcta atgaaggagg ttccgagtat gaatgaacac gatgaagcag aatggacaaa
    12421 aaatgaagaa caaacagaag atcatgaaga aacgaacact tcattggaag aacaaacaag
    12481 taatcctctg gatgacacag aagaaatccc tatgctagag gaaacaaaaa tacacgaaaa
    12541 agaaaaagta actccgacga atacgaatac tgcagaaaac acccatgctt acgaaataac
    12601 tgaaaatccc gggtatttag aaggaaaaga gcaagatcat caagatatcg tcagtttgat
    12661 tgaaacagcg gaagcagaat tagcggattt acgtatgaga aaagatttga tcgaatcaga
    12721 ttttccacaa ttattagaat catatgatcg ttttatattg aatgaagaac caatctccac
    12781 gatcggaaag acaagcctga tcgaatactt cacgtatgaa ttactgaaac cgttgaatga
    12841 tattggtctg gaacggtcaa attcagctta tgacacatgg aaaacaagac atttttcgat
    12901 caattatacg ttgaatggac aaaacgaatt agtcttttcc tttgtattac cgaccatcga
    12961 tcaacgatac caagtggaat cgatgagtct cctaaacgta tcaccagaga caatggaaat
    13021 caatgtccaa gatgaccggg tgctttcttt gatccgacac tggtcagtgg atcgagtatt
    13081 ctctgcaggg cagatcacga tattcaatca caaattgaat caattattag cccatgcaag
    13141 aacacttggt tttaccgtta atcagacatt actagacaat acgaagccac ttcgtttgaa
    13201 tcttcagagc gaatttgaat tgacgaatga agtattggat gatattttta tcgtggcaat
    13261 gaaaaatcca tcctacgata tggaaaagat caacgaaact acttatcaag tacgattaga
    13321 taaaggacag tctttaacga taacaacaaa cgaaaaagat caaacagtct tgtcgatctc
    13381 atctggagac tacccacgat ctgtcattga tttctttatc aattacgaat tcttggtccc
    13441 attaatggtg agaaagtcct gattggataa gttggattac cagatgtcat tcagataatt
    13501 ctgttccaaa ggacttgcgg cagttatcca gaataattgt cacaagtctt ttctgtatgt
    13561 actaaatgga cgtgaataac ttgctgatga aggagggatt tcattaggaa tgtccacaaa
    13621 taaaatagca cagcttagct tattgtctgc tgcttgtgta gcaggtagga tcgcttttca
    13681 atttattccg aatttccagc caatgactgc tattttttta tttatagtac tttacttaag
    13741 cttaaaagat gcgttaattg tcatgagtct ttctattgcg atcagcagtt tttattttgg
    13801 tgttggacca tgggtcatcg gccaatggat cagctatact gttgttctga gtttgttttc
    13861 aatcgtctgc ttgagaaaaa cagtgcaaag gaatctatgg ttaaagggag tctgtttttt
    13921 tctggcgggc atggtttacg gaataggtat gaccgttttt gatacgctgt tatatcgttt
    13981 gcctcaacct tggatctatt accttcaagg ggtgtcgttt gatttgatgc acagtatagg
    14041 caatgttgtg ttctttcttg tttttctgcc agtcgtcaaa cgtttttaca atcattcagg
    14101 aggaaaaaat gaaaaaaata atttatagtt tgcttgcaat gagtgtcagt atactcattt
    14161 tgacaagttg cagtacaacc tatacacaat cggcttccaa ccaaaaggag gaagtaaaaa
    14221 catcgcaaaa agcaacaatc acgttacaag aaaatggtca aaacttttcg gaaaaagaag
    14281 tgttcttcaa aaaaggcgat gatttactga gtattttgaa acgaaatttt gaagtcaaag
    14341 aagataacgg cttcatcact tcgcttgaag ggcactcaca ggatgagaaa aatcaactct
    14401 attggatgtt tactgtggac gggaaaagtg ctactactgg tgccaaagag atcaaattga
    14461 atgatggaca atcggtcatt ttcaatttgt ctaaactata agtttcataa aaaactgtta
    14521 aagggcgaat attgccataa ttcagtgagt tcagttgatg aaagaaggaa aataacatgg
    14581 aaaattttaa aaaaagcgga atgttttccg taattgctgc tctaggggct aatattctag
    14641 ttgctatcag caaatttgtc ggctatgcaa tcagtggcag tgccgccatg ctaaacgaaa
    14701 gcatccacag tatcgttgat tgcagcaatc agattttgct gttattcggt gataaacaag
    14761 ccaataaagg acagagcgag ttgcatcaat ttggtgaagg acgagcaaaa tactttttca
    14821 gtacaatcgt cgcaatgatg cttttcttcg gcggtggggc acttggggtg atggaagctt
    14881 ttgaaaagtt gacgcatcca tcccatgaag tgggtaatcc gatcattgtt atagcaatac
    14941 tgttatttgg tttggtaatc gaaggaagtt cgttacgcgt ggcaatgaaa gaaatcaaag
    15001 aattgaatac tgaaaaactt tccacgatgc gctttttgcg cgaaagtcga cacagtgaaa
    15061 tactgattat ttttacggaa gacttgtgtg ctgtaatcgg tttggtcttg gctttagcag
    15121 gaacaatatt gacttatgca accgggattg ctgcatttga tgcgatcagc ggattgctga
    15181 tcggtttctt gctgatggga gccgccattt tcctagcaaa agaattttat agtctgatca
    15241 tcggtgaaag cgtgacagca agcgatttat caaaaatcaa gttagctttt gaacgtcctg
    15301 agatcagtcg gctgatcgat ataaaaacag ttcatttaag tccaacggag attttagttg
    15361 cagcaaaagc agatatcgtt ggtaacaaag agaatgaagc ttactcggtc atcaatgata
    15421 ttgaaaaaaa tatgcgtcag tctcttcctg acaagaagat gtatatatac attgaaacag
    15481 ataaatatga cccaaattat tctcgtaaat aaaacgttta ttcatgaatt gaagcaaaaa
    15541 tgagaaaaat ataatcttgt ttttaaataa aacggctaca atttagcgaa tttatgttac
    15601 aatgagtccg ctgtttattt gttgtaatta aaggagatat tttaatgaac aaaaagaaaa
    15661 gtgcgccatg gcttcaagct ttgcatgcag tgatgccact ttgtataagc tatataccag
    15721 taggattagc ttgcggagta ttgttgcaaa aagttggatt tgatcctctc ttatcaggac
    15781 tgttatcgat tctggtcttt tccggtggcg cgcagttttt agttgcatct atgcttacga
    15841 cgcaagcatc atttgcaacg acattattga tggttttctt tttagaatta cgctatacat
    15901 tattaggagc aagtcttgcc ggttttatga aaaaagaaaa gcggacgttt ttggctgtct
    15961 tttctcaatc actgaatgat gaaaactatg cggtgaacta cttaaagttt tcaactgacc
    16021 cttcctggaa taaaagaaag gctttatatg tcaattggtt ctcaatgact gcatgggccg
    16081 gtagcaatat gatcggtaat tttttcggct cggtgattcg agtagatgct gaccttgtcc
    16141 attttgcttt gacggccatg ttcattttca tgtttatcat gcagatgaaa agcgcactac
    16201 tgatctttac aggtttattc tcaggcgtat taggggtcgt attcatggta ttattccaaa
    16261 atactttcgg attgattgca gcaacagtca ttgcttcatt tgccggattt atgctggaga
    16321 aggcattcaa aaaagaaaaa gctaagaaac taagagaccg cggtaaagat gtcaatgagc
    16381 cgctcttcaa cttcccggaa caggaggcca atcaccatga ctaaactata tttgctactt
    16441 ttagttgctt gtctgttcat cgtggcatat atcccgcgct tgttcccgat gctttatttt
    16501 actcatcgaa aagtgccatc ctggttcagt gactggatga aatatgtacc ggtggcatta
    16561 tttgcggcgt tagcttttaa agatgttttc attactcatg aacatctgga tattgcttgg
    16621 aatatcaaaa tagctgcaat gattctagta gcaggcgtgg cttacaaaac acgttcgatg
    16681 gcgttgtcag ttttgacagg acttgcgtct gtctttttgc tgtctatgct ttaaactgtt
    16741 ttattttatt aaaaacagag attgcgacat aagttgttca aactgatcta tccgaactgc
    16801 atttgcaaca gtcaattctg gatgcaagtt tattcgtagg gataaaaaag attagtgttg
    16861 gcttatgatg caagctcttt tttattttag gaattagaga gggatttgtc cttctatgat
    16921 tttttgctat aatagggaag atgtttattt tatgaaagaa tagttgaggg aatttaaatg
    16981 aataaaaaat tgatgcaagg aacattttgg ctaacctttg ctaatcttct atgtaaagtt
    17041 ttaggggtag tctatttgat tccctggcta agtatgatgg ggaacaatca agatggtatg
    17101 cttgctacga cattgtataa tgttggctat cttccttatg gattgttcct gatgcttggt
    17161 acagtcggat ttccaaatgc gattgcaaaa aaagtagctg tagccacaaa agaaggagat
    17221 aacgctgctt gtcggctgat ttttagaagt acgatcaata tcatgtttgt catcggtatc
    17281 gcctctgctg cactgatgta tttatttgct cctttactag ccggaatcag tccgattgct
    17341 aacgttaata atgggatact agctatcaga agtttatgcc catctttgat tgctatccca
    17401 attcttagtg caatgagagg atatttccaa ggaaaaaatg atctgcggcc ttatggaact
    17461 tctttgatta tcgaacaggc tgtgcgagtg atcgttatct tggctggtac ttattacttg
    17521 cgcgtattga ctgacggaac catattagaa gctgtattga tcagtacagt cgcttctttc
    17581 ttcggtgggt tagctgcgat tatccatatg tatatcgtcg gacgcagaaa agacttcttc
    17641 gaacttaaag attttttgat ttccagccgt tatttcgaaa gagaaaaccg atctgcctct
    17701 gtctctatca ttcgggaaac actaccgttt atttttgtcg gctcagtcat tactatcgtt
    17761 caaatgatcg accaagtaac gatgaaacca ctgcttcact ttttccgtcc agagatcgca
    17821 gatcagcaac tggaattctt attcagccgt gcttctgtca atccaaataa attgacattg
    17881 atcctgatct ccatggtagg aacagtagct ataagcagct tgccaatatt aagcacgatg
    17941 aagaaaaaag atcggataca aatcgaaaaa acagtaggag atagtttttc gattgcgtta
    18001 ttgattcttc ttccttcatt gactggaatg tctttattag ctggaccact atacacattg
    18061 ttttttggct atgatcctga aagtgtcgga tatttccaaa tggctttgct agcttctttg
    18121 tttttctctt tattcacgat cctatctacg atgcttcaat ctttgaatca ccatcgtttt
    18181 gcgatccgat taactgtaga agcaattatc ctgaaagtca tcttccaagt aatcggatta
    18241 ggattagttg gcggatacgg gatgagctta tcaaatggct tagcattcgg tattgtcttt
    18301 gttcgcggct atcactttat gtgcaaagaa taccgaatag cacctttagc aaagatcagc
    18361 aattttttct taaaaacttt ccgctccacc ctgattatgc ttgcagtatg ttttgccgtt
    18421 ttctttttgc tgaatcagcg attgtcaatg gaatcaaaaa gctatgcaat gatttattgt
    18481 gtggctgtag gaagtattgg cggcttagta tttttatttt ctcaattcgg aaaaaatggg
    18541 tttcgaatgt tgaaaaatgt taaacatcat agcagaagca aagaataggt taactaatcg
    18601 ttttttgctg tcacttgaaa aacggcaact cgtatgatta cgcttgtcgt ttttctattt
    18661 tccaatttta gtttcatatc taatcttact tatgatatag ttataaggtc tggaaaaatt
    18721 ctttgaaacg aggaaattaa acacatgaaa cttgcagtag ttacagatag ttcggcatat
    18781 ctgccggaac gtatcaagaa ccatcccgac ttattcgtta ttccaatccc tttgattatg
    18841 gatgggaaaa tttataacga aggaatcgat atcgaagcgg atgaatatta tggactttta
    18901 aaaaacagta aagattttcc tactacttca caacctgtcg taggggaagt tttgggttta
    18961 tatgaggaat tgaaagagaa agggtatgac acgattttaa gtatccatct ttcttcaggg
    19021 atttctggct ttgtcaacac attatttgcg atgaaagatg acatcagcgg agtaacggtc
    19081 attccttatg actctaagat cactagtatg ccgatgggac acatggtaga agctgcctta
    19141 gacttaaatg atgaaggtca gtcccttgat gaaatacttg cacatatcga tcgaatccga
    19201 gataatacct acgcttatct gattgtagat gatttgaaca atttagtacg aggaggacgt
    19261 ttgacaaatg gtgcagcgct gattggcagc ttattaaaaa tcaaaccgat cctgacattt
    19321 tctgaaggga aaattgtttt attcgaaaaa atccgttcaa gtaaaaaagc atttgctcga
    19381 gctgaagaaa ttattggtag aagaaataac gaaatacaac cgccagtcaa actttacgtg
    19441 atacatgcaa ataatttaga agtagcgatt gcggagaaag agaaattgca gaaaaaatat
    19501 ccaaatgcag aaatcgaaat tggtcatttc ggaccagtca ttggtacaca tttaggtgaa
    19561 aaagccatcg gcattgccat ttcagcgcaa taattccttt tattcaaaaa acataccaaa
    19621 aatattccaa catcatcttg gactttctac atgttactaa tgagcgtgta aaagaacgag
    19681 atggtgtttt tatttctatc aatgaagctc tttttctttg caatcctcac ttcaatcctc
    19741 tataatgtcg catatagttt agtaaatgag taaactacta atttacttat ttactaaaaa
    19801 taaaaggatg aggagttaaa ataatggaaa taccaacaaa tttaaaacat aaaccagtaa
    19861 tcgttgtaga agactatgat aaagttgatg gaagaaatgc attaaatacg gatgcaaaag
    19921 ggctttcact gggccttgct cagtggaatg atcgtggaaa agtcgatatc tcaggcaagg
    19981 tatggcgaca tactggagaa aaatggtcac gacagtcgga agaattacca atcacacgta
    20041 ttttagattt agcgatctta gctgcacaag gaagtttata cttccaagat gcttatcgtt
    20101 atgaaaaatt ttatgatcca gaaaatccag tcgtagacat cattggatta caagggaacc
    20161 gaatgacggt agaagtagat accaaaaatc caaagatcga tgaagacatc attttgtatt
    20221 acgatacact acaaaaagac ggagaactga ttggggaacg attccgtatt ttaaaacgtc
    20281 ttttagatga gctaggatat taatagacag agatgatcaa aaggggaaga gaagcatgga
    20341 gaatttctca ctaacagaaa accgaacctt ttcacggcta gaaaagcaaa tgatctggaa
    20401 gaaaccgaaa agccatcgga caagtgcaga agaatggcgg atcgcctccg aaatcaaagg
    20461 gaattggcaa gatcctgaaa tgaaaatcag taacgtactt ttggaaggag atgccgggtc
    20521 ggggaagaca caattagcca aagcactatc tgctgatctg caacttccct atacaaaagt
    20581 gacctgtttt gcagatatgg acaaatcaga tgttttcgga gccttattac ctgtagtaga
    20641 ttctaataag gaacaagacc aagaattact agaagctatt taccaaacag atacacttga
    20701 agctgtactg acactgatcc agaaccatta cggcattgat cctttttctg ctaaagaaaa
    20761 attagcagag ctagttcaaa ggatcgaagc tgattatcca gatgcaatcc attacagcta
    20821 ttacccttct gaaatcgttc gagcgattga aaaaggatat ttattagaga ttcaagagcc
    20881 gactgtcatc cgagatgcct ctgttcttgt ggcacttaac tcagcgctcg aaccaaatgg
    20941 gatgttgaat ttaccgacag gaatcattcg tcgccatcca gattgcgtca ttgtgatcac
    21001 tacaaatcga aattaccaag gaaatcgccc attaaatgaa tctttaagag atcggatgca
    21061 acatgcggaa aaaatggatc ttcctgcctt agaagtcatg gcagaacgtg cgatggcgaa
    21121 aactatgatc caaaaacaag aacttctctt aaagatggca gagattatcc gtttgttgga
    21181 tgaaactgca aaagctaacg ccatcaaagg tgtagcagga atgcgttctt atttttattg
    21241 ggcaaatacc tataagcaag gacaagattt actccagtcg atttatccaa aagttttgta
    21301 taaattatcc actgattcag atgaattggc aattcttgaa caagctttgt ctgacagtgg
    21361 attgctactg gaacttcagg acatattgag aaaagaaaaa tggggtcatc ctcaatctga
    21421 gcatacaaaa ggaagaacca ttagcgccga agaagcaaat gaacgaaaca tcactgaaaa
    21481 tgctgaagaa attctgcgct tagatgaaaa gaaatcgttg tctgctgaag aaccaaatga
    21541 gggagaagag aaacaagaag aaacaataga aaaaaacgag attcctcctc aaatgcaaaa
    21601 agaacgcagt agcgatatcg aaggaaaaaa tcaaaaagaa caacaaacag gagatgattc
    21661 tcaagaaatg tctgcttctg acatggaagc acatgataag gaaatcaaga agcaactgaa
    21721 taaagaagcg cgacaaatca tgaaggaaac gattcatgaa aaagaaggtc tcatcgttca
    21781 ccgaccagct ttccaaggaa aaacgccgga agcacgtgag ctacacatgc aagtaatgcc
    21841 aatagtagaa tcacttagcc ggcaaatttt agagttactg gataatgaac aaacagagac
    21901 gtaccaaaaa ggaaaatacg aaggtcagcg gttcaatgca agcaaagtag catatggtga
    21961 tttgagaaat tttgacaaaa agaacccgcc acatgagcaa ccttctttag cggtagctgt
    22021 acggatcgat gaatctgggt caatgatacg ggatgatcgg atcgaagcgg caaaaaaagc
    22081 agcgattgcc attgccgaat tttctaagaa agtcgatatc ccattgttga tttacggaga
    22141 tacagcagac cgttcgtcaa gagaaaagac ttctttgttt tcttataaag aatttgaaga
    22201 cggatttcaa tggttaaatg aaaaattagt cactatgaaa ccaaggcaaa acaatcgtga
    22261 tggtgctgca cttcgtttag cagcagaaaa attaaacaga caatccgcaa caaccaaact
    22321 gttgcttaat attagtgacg gtcagccaaa agcgttgccg gattatacag gcaaaaaagc
    22381 aaaagaagat atccagtctg ttttgaaaga atatgaacgt caaggaatcc tatttgtctc
    22441 cgcagctatt ggccaagaca aagaagaaat caagtcgatc tacggtgaaa gtcgttttgt
    22501 agatattact gacttgaatg aatttcccaa gcaaatgatt caactgattg cccgctatct
    22561 atgataaaat aaagctcaac gaacgcactt agcagattcg ttgagctttt cgtttctagt
    22621 taccggtaac aaggatactt ctaaaataga atcggttata atcaagaggg accgttaaag
    22681 gagtgaaacg atatgataca agaacaaaaa tggtggaaag aagcagtagg ctatcaaatc
    22741 tatcctgcta gtttcaaaga tagcaatggc gatggtcaag gagacatcaa tgggatccgt
    22801 gaaaaactta attatttgaa agatcttggt atcggcttta tctggatcaa tccgatctat
    22861 caatctccat ttgtcgataa tggttatgac gtaagtgact atcaagatat agaagagaga
    22921 ttcggaacga tggaagattt tgatttattg ctaaaagaag cacatgaatt agggatcaaa
    22981 gtgattatgg atcttgtcat caaccatagt tcggatcagc atcaatggtt tgaagaatcg
    23041 aaaaaatcta aagaaagccc gtatcgcgat tattacattt gggtcgacgg ggtagatggc
    23101 aaagaaccga ataattggac atcgattttc ggcggttctg cttgggagta ttctcatgaa
    23161 actggacagt attatctgca tgtttttgca aaagaacaac cagacttaaa ttgggaaagc
    23221 gagaaattga aagaagaact attcaatatg atccgatggt ggttggataa agggatagat
    23281 ggatttcgtt tagatgcgat ttctcatgta aaaaaagatg aatattcagt aaaagccaca
    23341 gagaaccctt tctcaccctt tcaaaatgtc tcaggaatcg aagaacattt gaccgatttg
    23401 aaacatgttt ttgaagagta cgatatcatg actgtcggag aagcaagtgg tgtcactgca
    23461 gaagaaggac cacaatgggt agggaaagac ggttatttcg atatgatatt cgaatttgat
    23521 catatccata tctggcagca agaaaaagaa gggcaattag atgtccttaa attgaaactt
    23581 gcattaagcg cttggcaaac ttcattggat ggtatcggat ggaacgcttt atatatggaa
    23641 aaccatgatg ttcctcgtgc cgtttcagtg tttggagata ctcgtcccga tttttgggct
    23701 atgtctgcaa aagcaatcgc tatgatgtat ttctttttgc aaggtacgcc atttatttat
    23761 caaggacagg aaatcggaat gacgaatatg ccctttgaat cgatcgatca agttgatgca
    23821 gtagattcga aaagacttta taaaaggctg cttgcggaag gtaagacaag agaagaagca
    23881 ttggatatca ttcgtgaaac aacgagagac aacagcagga cacccatgca atggacttcg
    23941 gaacaatatg ctggtttttc tacacatgaa ccttggctga tcactaatcc aaataccaaa
    24001 acgatcaatg tagaacaaca agaatatgaa ccagagtcgg tcctgcaatt ctataagaac
    24061 atgatccgca ttagacaaac caataaaggt ctgatatacg gttcttataa agaatatctt
    24121 catgaacatc cacaacttta tgtctatgaa agatatttag aagatgaaca atatcttatt
    24181 atggtaaact taacagaatc acttgctgac tatgaacttc caaaagaagc agatcagtcc
    24241 tggacattac tcttgtcaaa ttcctctagc ggagaatttg aagctaaagg catactagca
    24301 ccttatgaag ctcggttata taaaacaaac aagtaaaact tcctcgttaa agttcattag
    24361 ttggactttg cgaggaagtt tttcagattg ctctgtcaat tactcatttc aataattcgc
    24421 tctaggacca ccgatatatt taggacgaga gcgaagggct gtcacgtgag cttgtccaag
    24481 ggttttcaaa cgaggtctga caggtacttg gacatgtttg atatgcatac cgattgatgt
    24541 gtcaccgata tcgataccgg cttgtccaac tacgtgttcc acttcgacag gatcattgaa
    24601 ctggtcaaaa gcagcaacag aacaagcacc tccagcatgt agtgcaggaa caacagagac
    24661 aatttcgaaa ttttttgctt ctgccactgt tcgttctact actaatgctc ggttcaaatg
    24721 ttcacaacct tggacagcta gcgcaatttc tttaggatcc aacaactctt ttaacgtacg
    24781 gatgatccat tgaccaactt ccgcactgga attttttcca atatgtcctc caacgacttc
    24841 gcttgtgctg catcctaaaa caaaaagatc accttttttc aagtgtgcct cagccaatat
    24901 gtcattaact atttcagtca attggtcttt taattctttt tctgacaact tcattttcta
    24961 aaaccttctt tttcaattaa tattgcttcc gtgcttgaat acgttgaaga cttgcagtta
    25021 taggaaaggc aagtcccatt ccaatcagat tttggaagat gtttcctggt atagaagcca
    25081 ctccagccgg ccaatcatat aaaatccaac cggcgatacc atagccgatg atcataacta
    25141 agcttcctaa taaaagccct aatccccaca tacgagtaga agatttgctg aaagaaccag
    25201 caacatatcc ttgtaacccg tggatcacta atgaaaaaac tgcccactgc ggatatcctg
    25261 aaatcaaatc gatcaataga ccactggaag caccaacaac taatccaccc gttggaccat
    25321 aaagaaaagc agcggtatag atcccggcct cgcataatgt cacaaatcca ttcgtagccg
    25381 gtacagggat caaaattgtt aatgaaaggg caactgtcaa cgcaatcagc atagcataga
    25441 tcgttaattg cttagttttc atttctcatt cctccttttt gaataccgcc agaccagaca
    25501 cttccggtat gatcgttcat cagcacgcca ttagaaatac aatgggcaac aaatttttta
    25561 ctttctgcta ctgctttggt aaaatctgcc cctaacacaa gattcgctgt tattgcagaa
    25621 gaaaaggcac aaccggcccc gtttactgtt gtagtgtcga tttttttatg agaaaatacc
    25681 tcataatgat caattgaata gagaagatcc acagcttcat ctccagccat ccttgtgccg
    25741 cctttgatca caatgttttt tgttcctgaa gcagtgagtt ctttagcgag atggatcatt
    25801 tctgttaacg aagagggaag agcttgacca gaaagtaaag cagcttctgc caaattaggc
    25861 gtaatcatcg taacaagtgg aaaaagttct tcgatgagct gcgaaatata caattgattg
    25921 atcacagctt cagtttcttt gaatgccaaa acagggtcta aaatgattgg gatattttgt
    25981 tttgttttta tgaagtctcg aacaaccatg attgcttcac ttgaattcaa taacccaatt
    26041 ttgataccat ctaatgaagt cattttatca atcgaaacta actgtcgttt aagcaattcc
    26101 aaagatatag attccagcat aaatcgctca ttaatatcta gtgcccctat agaagtcaag
    26161 gcgcttagtc cgaatagacc aaaattctca aaagttttta aatctgtttg gattcctcca
    26221 ccagcaaatg gatcagaacc gccaattgtc aagattcttt tcataacatt ttcctccatg
    26281 aataagagta taacgaaatt ttcaaaaaag aaaaacagcc aattggcata tttttatctc
    26341 atccaattta aaaaaacaaa aagaaaagaa agaccaaaat ggatctttct ttccttaaat
    26401 agttaggact tttgtgattt taattctttc tcgatttgaa gccgttgcac tttcattgta
    26461 gctgttcgcg ggattgcatc aaaatcacgg atgataggga cgttcaagcg aggaagttca
    26521 tggacttgct cccaccatcg attccaatcc atttctttat caggagctaa agcaataatc
    26581 ggttgtggag aatgattttt atcccttaca atcactactt ctgccaaaaa atctaacgaa
    26641 tctaataaaa agtcttcaat agctaatgta ctattgatcg tttcgatcaa atccacttgc
    26701 cgatctttca aaaataggtg gccatgctta tccataaaac cgtaatctcc gctatcccac
    26761 caatcgccat aaacattttc ctggaaccgt gcatcttcct tgtaataagt caaagcccgt
    26821 cctttagaca gtaattgtat atgaccatct gtgttttcag gtaggacatt cccaactgaa
    26881 tctgtaatcc gagcgcttgt catgtcttcc agtccaacgc ccatatctct tgcatcactt
    26941 gttttcaaac tctctagtgt atgagcctta agaatcattg gtccgcattc actttggcca
    27001 tatacttgaa gaaataccgc atcctgtgcc atcgacgttc gcaaaaatgt cttcattgtc
    27061 tggttgttta tagcatcaaa agtggagtga tagtatcgga tacccgaaaa agcttctgga
    27121 tgctccttag cagtaaaacg ccactgaaca aagttattcg gatgggtctc tagtgcaatt
    27181 ggccggtgag cttccaacat atgaacaact tttgaacttt gtgcgtttgc taaaggcatc
    27241 ataggaaatc ccatggacat caaagaagag ataccaatat tgaatctgga atgcactgga
    27301 gagatgtgaa aagcgattaa tttcttttcc gaaatttttg tgaaaactgt tttttgccat
    27361 ttcgtccgcc atcccataga atgagcagaa tgacaaataa gtttagggat accagttgta
    27421 ccagaagtat gggtcatata agaaatttca tttttgtcta aaaatgattg agaaacagac
    27481 aaagcaggtt gttccattaa ataaagaata ggaa
//
LOCUS       DAZBSA010000031        26940 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000031 DAZBSA010000000
VERSION     DAZBSA010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.031"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            71..1141
                     /locus_tag="ACHGMT_001191"
     CDS             71..1141
                     /locus_tag="ACHGMT_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6834950.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            1156..1824
                     /locus_tag="ACHGMT_001192"
     CDS             1156..1824
                     /locus_tag="ACHGMT_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6834951.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            1844..2422
                     /locus_tag="ACHGMT_001193"
     CDS             1844..2422
                     /locus_tag="ACHGMT_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN6834952.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            2823..3734
                     /locus_tag="ACHGMT_001194"
     CDS             2823..3734
                     /locus_tag="ACHGMT_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFN6834953.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(4112..5029)
                     /gene="rnhC"
                     /locus_tag="ACHGMT_001195"
     CDS             complement(4112..5029)
                     /gene="rnhC"
                     /locus_tag="ACHGMT_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFN6834954.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            5231..5668
                     /gene="zapA"
                     /locus_tag="ACHGMT_001196"
     CDS             5231..5668
                     /gene="zapA"
                     /locus_tag="ACHGMT_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFN6834955.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            5726..6274
                     /locus_tag="ACHGMT_001197"
     CDS             5726..6274
                     /locus_tag="ACHGMT_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFN6834956.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            6389..8749
                     /locus_tag="ACHGMT_001198"
     CDS             6389..8749
                     /locus_tag="ACHGMT_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFN6834957.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            8836..9150
                     /gene="trxA"
                     /locus_tag="ACHGMT_001199"
     CDS             8836..9150
                     /gene="trxA"
                     /locus_tag="ACHGMT_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFN6834958.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            9332..11110
                     /gene="uvrC"
                     /locus_tag="ACHGMT_001200"
     CDS             9332..11110
                     /gene="uvrC"
                     /locus_tag="ACHGMT_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFN6834959.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            11255..12154
                     /locus_tag="ACHGMT_001201"
     CDS             11255..12154
                     /locus_tag="ACHGMT_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6834960.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            12147..13409
                     /locus_tag="ACHGMT_001202"
     CDS             12147..13409
                     /locus_tag="ACHGMT_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6834961.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            13570..15468
                     /locus_tag="ACHGMT_001203"
     CDS             13570..15468
                     /locus_tag="ACHGMT_001203"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFN6834962.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(15798..16517)
                     /locus_tag="ACHGMT_001204"
     CDS             complement(15798..16517)
                     /locus_tag="ACHGMT_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFN6834963.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    16707..16754
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            16854..17186
                     /locus_tag="ACHGMT_001205"
     CDS             16854..17186
                     /locus_tag="ACHGMT_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834964.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            17173..19176
                     /locus_tag="ACHGMT_001206"
     CDS             17173..19176
                     /locus_tag="ACHGMT_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFN6834965.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            19195..19665
                     /locus_tag="ACHGMT_001207"
     CDS             19195..19665
                     /locus_tag="ACHGMT_001207"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFN6834966.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            19686..20270
                     /locus_tag="ACHGMT_001208"
     CDS             19686..20270
                     /locus_tag="ACHGMT_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFN6834967.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            20285..21271
                     /locus_tag="ACHGMT_001209"
     CDS             20285..21271
                     /locus_tag="ACHGMT_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFN6834968.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            21261..21572
                     /locus_tag="ACHGMT_001210"
     CDS             21261..21572
                     /locus_tag="ACHGMT_001210"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFN6834969.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            21594..23375
                     /locus_tag="ACHGMT_001211"
     CDS             21594..23375
                     /locus_tag="ACHGMT_001211"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFN6834970.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            23368..24744
                     /locus_tag="ACHGMT_001212"
     CDS             23368..24744
                     /locus_tag="ACHGMT_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFN6834971.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            24751..25386
                     /locus_tag="ACHGMT_001213"
     CDS             24751..25386
                     /locus_tag="ACHGMT_001213"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFN6834972.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            25416..26771
                     /locus_tag="ACHGMT_001214"
     CDS             25416..26771
                     /locus_tag="ACHGMT_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFN6834973.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
BASE COUNT         8893 a         4563 c         5890 g         7594 t
ORIGIN      
        1 aatattcgcc aaaaatttat caagtgataa aattgttgta taaaatgatt atttcaggag
       61 gaggagttaa atgtttttag cattaaatga aattatgcat tcaaagttac gttatgcgtt
      121 agttgcaggg gtgatgtttt tgatcgccta cctggtattt tttttgactg ggttagctta
      181 tggcttagct caagataatc gaacagcagt cgacaagtgg gaggcagatt cgattgtgct
      241 ttcgaaagat gcaaattcaa atctagggat gtccatgatt actaagaaga tagcagaaga
      301 agtcgaaggt gggaaagtgg cgtatcttgc ccaaacacct ggagtcgtga ctagtaaaga
      361 cagtacagaa gaaggaaaaa tcaatgttag cttcttcggt atcgataaaa atcaattcat
      421 catgcccaat cttgttgaag gaaaagcttt tgataacgat gatgaggcag taggcgatat
      481 tagtttgaaa gaagaatatg gtctagccat aggagataca gttaagttat ctggtagtga
      541 caagacgttt aaactaactg gctttacgga tcatgccaaa ttcaatgtct caccagttct
      601 ctatacaacc atcaatgctt accaagaaat ccgcttcgaa aaagaagata caagtgaaaa
      661 tgcacggatt aacgcaatag ttgtcagagg taaaatcagt aatttacctg aagatttaga
      721 acagatcaaa atttctaaat tcatcaatga attacctgga tataatgcgc aagtattgac
      781 ttttggattt atgatcggat ttttgatcgt tattgcggct attgtgatcg ggattttcat
      841 ttatgtactg acgatgcaaa agattaatat ctttggtgta atgaaagcac aagggatcac
      901 aggaggattt attgcgcgtt ctgtagtggc acaaacattc attttatcat ttgttggtat
      961 tcttcttggt ctattaggaa cagtgggaac atcccttgtt ctacctgatg cggttccatt
     1021 ccaaagcaat tggctgtttt ttggcgtcat tagcttgttg atgttagtag tagctgtgtt
     1081 aggcgcacta ttttctgttc ggacaatcgt aaaaatcgat ccgttgaagg caattggata
     1141 agaaggagga agaacatgaa agcaatagaa tttaaagaag tagaaaaaag tttccaagat
     1201 ggcgaccaag tagttaaagc attgaaagaa acaaccatga gtatcgataa aggggaattt
     1261 gttgcaatca tcggtccttc tggttcaggt aaaagtactt ttttgacttt ggcaggcgga
     1321 ttgcagacgc ctagcagtgg ctctgtattt atcaatgatc aagaattcag taatcaaaaa
     1381 gaaagcgaac gcgttaaact acgttttaaa gaaattggct ttattttgca agcttccaat
     1441 ctggttcctt ttctgacagt aaaaaagcag ttattattag tagataagat taaacatgaa
     1501 catcgtcaaa aagaagcaaa cgaattattt gagcagcttg gcgtggataa gttgatcaac
     1561 aagtatccag aagaactatc tggtggagaa cggcaaaggg tcgcaatcgc tcgtgcattg
     1621 tacaatgatc cgtccattat tctagcagat gaaccaactg ctagtttgga ttcagaaaag
     1681 gcacgagaag tagtcgatat cttggcaaaa gaatcaaaag acaaaaataa agcaacgatc
     1741 atggttactc acgatacacg gctgattgac aaatgcgata gaatatttat gatggaagat
     1801 ggtgtattga aagaaaaaga ataaccaaaa agcaggtgga aaaatggtcc atcgatacaa
     1861 caaagataca cagcaaaaaa ttttgaaaga agcgactgac ttgtttatgt ccaagggata
     1921 tctcggcact tcaacaagag aaatcgcaca aaaagttggg atcacccagc ccaatctcta
     1981 tcattatttc ggtgataaag aaaaactata tacggcagtt ttagaaaacc acttaaaaga
     2041 tgttggaaaa gctttgagag aaatcgttca aacaagcgaa acgggatttc aagtaacctt
     2101 gacaaaaatg gcgcagtttt tgattgagac acatctggtc gatttgtttc tgatgcttca
     2161 cgatcttgaa agcaatcttt caaaggaaac aagagatcat cttttctttc tatggaagaa
     2221 aaattatcgt gaaccttttg aagaaatctt ttcagcaaac caatctgtgc taagaaatgg
     2281 gatttcaaaa gaaatcgcag caaggcattt tttcttagtg ttagcacctt acatcactcg
     2341 gtcgaaaaga cctgtggaag aaccgctaaa tgtggcgcaa ttgatcgatt tatatttgaa
     2401 tggtgtatta ggagaagcgt aaagctgtta tatagggaga aaatagttag gtgaagaagc
     2461 aagatatgac gaagatcgtt atatcttgct tctttatata ggtagttttt cataaataca
     2521 aatttgttat tctctcatga ttatcagggt atatttttat ttattatgtc ggtgttccag
     2581 gaagtgtttt aatttaataa cgaagaaatt aattgtttgt agaatgaagg aaactattta
     2641 ttgggtagat gaatcaccac gaatagtcaa aaggagattt caggatatgc caaaaatgaa
     2701 aaaaatcatt ttagcagtaa tgttattttc tacggttata tcttttacag cccctttttt
     2761 gcttacttaa gctgatttta agttttggcc cttagtctgg atctacgagg tgagagtcaa
     2821 gaatgaaaaa aataattgga gcttttgtat ttgcaatcat tatcattatt atctgttttc
     2881 aaatagatgt ttttggtcaa aaaaagatag ttggacaaac ggtcagcagt acgaacagta
     2941 cggttacaaa gacaaaggaa tcatttgatg atacagaaca agagacatta atcaaaatcg
     3001 gaaaaaatga ccgtacacta tatacagatg actcgctaac taccccactt gcaactataa
     3061 acggcggaga gctggcggag tttctttctg aaacaaaaaa tgcttatcag atcaagacaa
     3121 atgatggcta tactggatat ttagccctcg aagatggaac caaagtcaca aagaatatac
     3181 aaacaaaacc ggaagagcta tctgatgcag tgattgtatt agatcctggg catggcggaa
     3241 atgatactgg ggcattaagc aatgatgaac aaacagaaga aaaagatata acattaagta
     3301 cagccataaa agtaaaaaag gcgctagagg atgctggagc aacggtttat ttgacccata
     3361 ctacagatga attagtacag cttggagata tatgcgacta cagtgaagaa aaaaaggcag
     3421 atgtctttat cagtttacac gcagactcta cggaatatgc gaatgaagca actggaatca
     3481 cgacatatta ttactatgga caagaggaaa cattagcaca gacaattgct gactccttca
     3541 ctgatctgcc tctggattca agaggaatct ccactgggaa ctatcaagtt cttcgagaaa
     3601 atcttcaacc ttctatttta atcgagatgg gttatatgaa taatgatagt gatttaaagg
     3661 aattagtaac agacagttat cagcagcaga ttgctgcaag tttgacacaa gcattgacca
     3721 cttattttca gtaataaata agcccagcca aacaaaaata gctcctcata tttttcgttt
     3781 ggccgggctt attatcgcag atgcaagctt ttaaacaccg tttaaggaag aggtcgtgag
     3841 cctgccgttc aactctgagt cacaaggagc aatttcaaaa aacagcttct catgtttgat
     3901 gactattgtg acttgtgacc gaggagttgg catgagaaca ccgtttaaga aaaaggttgt
     3961 gaaatcaacg ttttgttctg agtattagag aaacaaacgg aaaaatagtt cctcatattt
     4021 tgccgtttgt tgagctaata ccgaggatca gatgattgaa caccgtttat tcggaacaag
     4081 ggactgtgac atgacttttg tcacagttcc attatttttt tagtagcttc aaagcttttt
     4141 ctgtattggc aaaatgaagt ttggcatatt tttcaagcat aggcatgccg cctttctcca
     4201 atagttttgc cgctacttgg tcagatttcg ttccagctcc tgacggaagg gtgattccag
     4261 cttcagctga tgctttgtct aactcttcca aaaagctggc acgactgatg atagaagcag
     4321 cagcaacagc taaatgatat tgttctcctt tagtaacgaa gtacagtttt tcttgtaatt
     4381 gatttttttc taaacgcgca tattttttaa aatttgcttc cggcgtaaac tgatcgatca
     4441 aaatcgcttc tggtttttct ggctcgattt tttttagcag caaatggatc gcttgattgt
     4501 gcaaagcgac tttcatccga acggcattgt attttggttg gatctcattg tatttttctg
     4561 gagataagac aagcagttta taggggatgg ttttttttaa gatgtcagca agctggatga
     4621 tttggctatc ttttaactct tttgaatctc taacaccaag tttacgtaat tctgcaagct
     4681 gatcacgttt cacataagca gcacatactg ttagcggacc gaaatagctg ccatttccga
     4741 cctcatcact acctaaaacg gataattgac tgatattttt tggcaaagaa gaggctgttt
     4801 ttgctggctt ggcttgttta tcctgtgcct ccccccattt atcagcttct attgctgcat
     4861 tcgtaccttg aaacatcact tttccggatg tataagcggt aatggtcgtc ccgtcttttt
     4921 ttgcagcaaa gctagtataa ggaatctgtt tatttaacaa ataagagcga tagtatgctt
     4981 tcatttgact aagttggtcg tttgttgctt tgataatgac gttgctcata aaattctcct
     5041 cgtttttata cataaaacta taccgcaact cacgactttt cgttttctaa gagattgcga
     5101 gacatagcat ttttttaaag tttatcatat aatagttaag tatgtgaaaa atatttgtgg
     5161 agaacggtga aagaaccaac ttcatggtac actaaataaa actttcatca aataggagga
     5221 aatgattcgt atggcgcacg aaaaaacacg ctacaaagcg gtgattgcca accaaacata
     5281 tactattatc ggacgggaaa ccaagcatca tatggatatc gtaacgaagc tgatcaatga
     5341 acagttagca gaattgaaac aattatcacc acaaatggat aatgaacaag cagcaatctt
     5401 gatggctgtc aatgcgcttt cggatcaatt gaagaaacaa gaaagaatat tggagctgga
     5461 agaagaaaca gcagaattaa agaaaaaaat gatcaaattt acagagcttg aaaatcgtgt
     5521 gaaacgcatt gaagcaatcg agaacgaagc tcgggaagta ttgaaagaaa atggtcaagc
     5581 agatcatacc attaaaaatc atgtggaagc acaacagatt ttaaacgaga aacgaaaagg
     5641 acaaattaaa caaaaatctt ctaattaaga tccttttatc aaaataagaa aaatttaaaa
     5701 aagttagaag agaaaggaac gtagcatgct gagtttgctg attttatttt tattgctaat
     5761 tgcgtttttt tctggagcac gtcgcgggtt tgctttgcaa atcatattcg cgattggata
     5821 tgtattgtca tttattgctg cccagcattt ttacaaacca ttagcaagtc atttggaatt
     5881 atacattcca tacccagctg tcaccccaac atcaaagtta gcattttttg atcaggtatt
     5941 tgcctttcac ctagatgaag ctttttatgc aggaactgct tttctgatta ttttgctaat
     6001 tggctggctg cttacccgat ttgtgggggt ttttgtccat ggattgacgt atgtgccgat
     6061 cttgagacag gtagactgga ttgcaggagg catcctgtca ctaatcatgg cttacgttac
     6121 tatatttttg attttgcagt tacttgcctt tgttccatta gatgtggtcc aaaatcaatt
     6181 cggcgagcat agtttcgctc gttttatcgt tgagagaacg ccatttctta ccaacaagat
     6241 gtatgagctt tgggtaacga atattttgaa ttaaggaaaa tagtgctgga aaatatttcg
     6301 ttttttgtac agagtagttt atttaagaag gctgcgtagc gtatgtccgg cacagctctt
     6361 ttttcatagt gaaagataag gtgataaaat gaataaccga atccttgaaa cattggaatt
     6421 tgaaaaagtc aaacagatgg tcagacaatt tgtagtgact gcacaaggaa aagaagaact
     6481 ggcagaattg gtaccagtga gtgagaaaca aacgatcaca aactggcttc aagaaactga
     6541 ggatggattg aaagtgcaac gtctgcgagg cgggatccct atacctaaaa ttgaaaatat
     6601 ccgtccccat atgaaacgga ttgagatcgg agcagacttg aatggagtgg agcttgcaca
     6661 agtttccaga gtattgtcta cgacaagcga attgaatcgt ttcattgatg atctttctga
     6721 cagtgaaatc gaatttgcac gtttgtatat gtgggcagat cagttagtca cgatccctgt
     6781 tttgagccgc cgtttaaagg aagcaatcga tgaagacgga cgcgtgactg atgacgcttc
     6841 tcctgagtta aagagcataa gacagaacat tcggcgaagt gaacaagctg ttcgtgaaca
     6901 gttagatggg atcgtccgtg ggaaaaatgc aaagtattta agtgatgcga tcatcaccat
     6961 gcgaaatgac cgctacgtta tcccagtcaa acaagaatac cgcggtgtat ttggcggtgt
     7021 agtccacgat caaagtgctt caggtcagac tttatttatt gaaccaaaac aagtggtgga
     7081 tttgaataat cgtttgcgtc aataccaaat cgctgaacga aatgagattc aacgtatttt
     7141 aagtgaactt tctgctgaac ttgttcccca tcgtcaagaa atcatccaca atacttatgt
     7201 gattggtaaa atggatctga tgaatgcaaa agcacgattt ggtaaagaag taaaagccat
     7261 cgtgccggga atcagtgaag acaatcatgt cgtcttgaaa caagcaagac atccactgat
     7321 cgatcaggaa aaagtagttt ctaatgacat cacaatcgga aaagactatc aagccatcgt
     7381 cattacagga cctaacacag gtgggaaaac gatcacgttg aagactttag gtctgttaca
     7441 actgatgggg caagcaggat taccgattcc tgcaggtgaa gaaagtcaaa tcggtatttt
     7501 tgaggaagta tttgcggata taggagacga acagtcgatt gagcaaagtc tatcgacctt
     7561 ttcttctcat atgacgaaca ctgtagatat tttatcaaaa gtcaatgaaa aaagtctggt
     7621 attatttgac gagctcggtg caggaacaga tccgcaagaa ggtgctgctc tagcaatcgc
     7681 aatcttagat gatcttggta aaaagtctgc ttatgtgatg gcaacaacgc attatcctga
     7741 attgaaagtt tatggatata atcgggcaaa tacgattaat gccagcatgg agtttgatgt
     7801 ggatacatta agtccaacgt atcgtttgct gattggcgtt cctggacgaa gcaatgcgtt
     7861 tgaaatctct agccgattag gtcttgatac agaagttatt gatgaagcga agcagttgat
     7921 gaatgatgag agccaagatt taaacgaaat gatcacagat ttagagaatc gccggaaaat
     7981 ggcagagacc gaatatctgg aaatgcgtca ttttgtttct gaggcacaag aacttcacga
     8041 tgatttgaaa gaagcttaca gctatttctt tgaagaacgt gaaaaagaaa tggaaaaagc
     8101 aaagaaaaaa gcaaatgaag tcgtgtcaga ggcggaagaa aaagcagaaa aaatcattgc
     8161 tgatattcgt aaaatgcaac agcagattgg gcaaggaaat gtgaaagagc atcagttgat
     8221 cgatgcaaag acccaattag caaatctaca tcaagaagaa actttgaaga aaaataaagt
     8281 attgaagaag gcgaaagaac aaaaaacgtt gaaaccggga gacgaagtgt tggttacgac
     8341 ttacggccaa agagggacgc ttttacgtaa aaacgggaat cagtggcaag tagaaatcgg
     8401 tatattaaaa atgaatgtgt ccgaagacga gctgacgcct gttgctcctc aaaaagagcc
     8461 gacacaacga gttatccatg ctgtacgatc tgaatctagt agtcacgtgc cgaatcaatt
     8521 ggaccttcga ggaaaacgat acgaagaagc gctaagtgag gtagatcaat atttggattc
     8581 tgccattcta gctggttatc ctcaagtgac tattgtccac ggaaaaggaa caggcgcact
     8641 tcgcaaggga attactgatt atcttaaaaa ccatcggagc gtcaaaagct ttgagtttgc
     8701 accggcaaac caaggcggaa atggtgcgac aattgttaaa tttaagtaaa gcttactaaa
     8761 ggtaagcaga tggttcgcta tccaagtata gaaatgctat actagtcata gattttaaaa
     8821 atggaggtct ttgatatgtc acaagtaatt acagataaga catttaatga agaaacagat
     8881 aaaggacttg tattgattga tttttgggca acttggtgtg gtccatgtcg tatgcaagca
     8941 ccgattttgg atcagttgga acaagaatat gatgaagaag aattcagaat tgctaaaatg
     9001 gatgtggatg aaaatccaga aactcctcaa caattcggta tcatgagcat tccaacatta
     9061 atgttgaaaa aagatggtca agtagtagaa aaagctgttg gtgttcattc aaaagaacaa
     9121 ctacgtcaaa tgatcgatca atatttataa aaacttacag caaaaataag ctgagggtgg
     9181 gactgaatcc agttccgtcc tcggtttttt tgcggttgag ttaaggttat tcaaattaat
     9241 attttttgag tataattacg ttgtcaggct ggattgtttt tttgacagac atgctataat
     9301 aactttaatt gaatatgaag gggagtacca gatgaacgaa cgaatcaaaa acaaattagc
     9361 tttacttcct gatcagcccg gctgttactt gatgaaagat aaaaatggaa cgatcattta
     9421 tgtcggcaaa gccaaaatat taaaaaatcg tgtccgctca tattttactg gtagccataa
     9481 tacgaaaacg gaacggcttg ttagtgaaat cgaagacttt gaatacatcg ttacagaatc
     9541 gaatatcgaa gcattgctct tagagatcaa tctgataaag aaaaatgatc ctaaatacaa
     9601 cattatgtta aaggatgata aaacctatcc tttccttaag ataacgaatg aaaaatatcc
     9661 ccgtttggtg attactcgta aggtactaaa agacaaagca ctgtattttg gaccatatcc
     9721 cgatgtcggt gcggcgaatg agacaaagaa gatcctcgat cgattatttc ctctgagaaa
     9781 atgcaaacct agtcagacga aggagccttg tctttactat catttgggac aatgcctgtg
     9841 tccttattat ttcgacgtag atccgaaagt ctatacgaaa atcgttgatg aagtgaaaaa
     9901 ctttttaaac ggaggacacg aaaaaatcga gtcagagatc catcagaaaa tggaaaaagc
     9961 tgctgaaaac atggagtttg aaaaagctgc agaataccga gaccagatcc gtgccattga
    10021 gacaatcatg actcgccaaa aaatgacaaa tactgattta ctggatcgag atgtatttgg
    10081 ttatgcgatt gataaaggat ggatgtgtgt ccaagtgttt ttcgtaagac aaggaaagct
    10141 aattgaacga gatgtttcaa tgtttccttt ctataacgaa gcagaagaag atttcttgac
    10201 ttacctcggt cagttttatc aggaaaatga gcatttcatt cctaaagaag tcttgattcc
    10261 tgatacgatc gacaagccca gtgtagaagc tttgttagct acgaaagtgc tgcaaccaaa
    10321 acgaggagaa aagaaaaaat tagttgaatt ggcgagtaag aatgcctcag ttgctttgaa
    10381 tgaacggttt gatctgattg ccagaaaaca ggaacggacg atcggagcag ttgaacgttt
    10441 aggaaacgcg atgaatatcc cgaccccaat acgtatcgag tcttttgata actctaatat
    10501 catggggacg aatcctgtgg ctgcgatggt cgtattcatt gatggaaaac cggcaagaaa
    10561 agaatatcgg aaatataaaa taaaaacagt caacggaccg gatgattatg catcaatgag
    10621 agaagtcatc tatcgcagat attcgcgagt gattcgcgaa gggttgcctt tacctgattt
    10681 gatcttgatc gatggaggaa aaggacaagt agatgtggca aaagatgtct tagaaaatca
    10741 attaggaatc gatattccta ttgctggcat ggtaaaaaac gataaacata aaacaaatga
    10801 gttgctattc ggtacagatt tggctgtagt accgcttgag cgaaattcac cagagttttt
    10861 cttactccag cgaatccaag atgaagtcca tcgattcgca atcactttcc atcggcagct
    10921 acgcagtaaa aacagttttg cttcccgatt agatgaaatt gaagggttag gaccaaaacg
    10981 aaaaaaaatg ctgcttaaag agttcaaatc gttgaaaaat atcacagcag ccacgacaga
    11041 agaattacaa gaaatcggac tgccgaaaaa tgtcgcacaa aacgtatttg ataaattaca
    11101 tcaggaatga ggaaagggca ctttacaaag aaagtaaagt gtcctatcat tttatgtaca
    11161 acaaaaagag cgtttacgtt ttgttggtaa ataataaatc gcttacgtct ggtgtctaag
    11221 gatatatgtg atttcttgaa gaaggagtga agaaatgtta gaagtcaagg atcttgttaa
    11281 aacttttggg aactacacag ctgtcgatca tgtctctttc caaatccccg atggaaaaat
    11341 catggggcta attggccaaa acggggctgg gaagacaacg acttttcgtt tgatcttgga
    11401 ttttttgaca caagaccaag gagaagtgtt atggaatggc catcatcttg gggagaaaga
    11461 ttatgatgtc atcggttatc ttccggagga acggggactt tatccgaaag taacgattca
    11521 agaccaactg ctttattttg cagaacttag agggaaatca agaaaagaga ttgaaccaaa
    11581 aatcgacttt tggatggaaa aatttcaggt aaaagggaaa aagacggata aagtaaaatc
    11641 tttgtctaaa ggaaatcagc aaaaagtcca gctgatcgcc acgttaatcc atgaacctaa
    11701 attgatcatt ttggatgagc cattcagcgg gttagatccg gtaaatgctg aattgctaaa
    11761 agatggaata ttggaattga aaaaacatgg ttcatgtgtc attttttcta gccacaatat
    11821 ggataatgta gaaaaaatct gcgatcattt gatcatgttg cgaaacggaa aaatggtttt
    11881 gaatggaaaa gtacatgaga ttcgtgaatc atttggccgt acgaagttat tcttggaatc
    11941 tggtttaagc caacaggaag tagaagaaat cgaaggcgtg aaaaaagtga tccttcatga
    12001 agacaatacg ttagagatta ccttagaaga tcctgaagct gggaaagaaa tctttactcg
    12061 tgcgactcag ttcggctaca ttcctatgtt taaccagcaa ccgccaactt tggaagagat
    12121 tttcaaattg aaagcaggtg aaatcgatgc gtaagttttg gattattacc aaagatgtct
    12181 atttaaaaaa tgttaagtcg atttctttct tgatcatgat attagtccct tttatcatgc
    12241 tgggtatcat ttatgtagca ggaaactttg ctcaacaaaa tagtgatgtc aataaaatcg
    12301 gcgtattggc tgataatgag caaatcagtc agcaaatgag ccaagtcaaa acagatgatt
    12361 ttgagttcaa agcagttgat tctgaagaaa aagcacaaaa agagctttcg gaagaaaaaa
    12421 tcgatgcgta tttagtagtc tcaactgaga atgataagat aaatgggatg ctttatagcg
    12481 aaaattcatt aggacaaagt acacaactac tgatacaaca acaactgact ggcttgcaat
    12541 cgatgcttcg agcgagtcaa ttaggcattt caccagaaga agtagctagc ttgagccaac
    12601 aagcaagttt gactcgacaa aaagtcaatt ttgattcaaa aggaaaaatg acagtcggag
    12661 aagataactc gacagtccaa tacgtcgtaa gttatgtagc aacgattgtc ttgttcatta
    12721 ttattttgac gtatgcgcag atcattgcac aagagatcgc ttctgaaaaa ggcacgcgga
    12781 tcatggaagt gatcctgtcc agtacgactg cacaaaagca tttttacgga aaattaacag
    12841 gtgttttact tgttgcatta acgcaaatgg cattatacgg tattattttt ggtataggat
    12901 acagtcaatt caaagatatg gaaatggtca aaaatatatt agacggtatc tcattggata
    12961 acttgattgg accattcttg tggtattctc ttctattcat gatctttggc attttgattt
    13021 tttctgtttt agcagcttta tgtggctctt tggtcaacaa agcagaagat acagcaaaag
    13081 caattttgcc agtgacttat ctatctctag gcggatacat gcttggattg atactaggag
    13141 cttcagatcc aaacaatatc gttattcgga tcacttcgta tattccattc ttatcttcat
    13201 atattatgcc gattcgctta gcaaatgaga cagtcggtat gggtggagca ttgatttcct
    13261 tgttggtact tgtagccgta acattcttat tgatgatctt gtctgcaaac atgtacaaat
    13321 cgaatgtact cgtttatagc gaaggcggat tatggagttc attgaaacaa tcgatttcaa
    13381 ttatgaggaa tgaacgaaaa aaagcataaa acaatcctaa aatacagaag gaagctggac
    13441 atctattgtt cccagcttct ttttgtatag acctatttta tttattttac tatattggat
    13501 atatattcat tgaaaatagt gtataacaac tttataatat tgagtatacc aatttttgga
    13561 ggcgttttaa tggaccgtta tttagattta ctttcagaaa aattcccctc aacggaggat
    13621 gtcatcacgg aaatcatcaa tctcgaagca attttgcagc ttccaaaagg aacagagtta
    13681 tttttgagtg atatacatgg cgaatttcct gcttttgatc atattctgcg tatcggttca
    13741 ggaaatctta aagaaaagat caaagacctt ttttctgaac aaatgacgga aacggatatc
    13801 agccaattca ctatttttac ggcttatcca gaatatgcat taacgactga ttggtacaaa
    13861 gaacaagata aatcgcagct gatccatcat ttgatcgatc ttttacggtt tacgacagtc
    13921 aaatataccc ggtcaaaagt tcgaaaagga ttgccgaaag aatatagcta tatcattgag
    13981 gaactcttgt atatcgatga ccgtatcaat ggaaaaaaag actatgtaaa aaaaatcatt
    14041 gaacaacttg ttttgatgaa ggaagaagaa aaatttttaa cgaaacttgc acagacgatc
    14101 caaaaatctg tcatcgatca tttgcatatc gttggagata tttttgaccg aggaactcaa
    14161 gcagccaaag tgatggatcg aatcatggat ttttcttctg tagatatcca atggggaaat
    14221 catgatattt tgtggattgg tgcgtattgc ggctctgagg catgtttact gacactttta
    14281 cggattgcag cacgctataa ttatttattt gaattagaga aagaatatgg gttgaattta
    14341 cgtccattgt tttcttttgc tcatcaaact tatcagaaga atccagtatt tgcgcctaaa
    14401 aacagtaaaa atctatccga aagagagcaa gaagaattag aaaaaatcca tcaggcactt
    14461 tctatccttc aattcaaatc agaacatcag ttattggatc gtcgacctga atttaaaatg
    14521 gacgatcgta aattgctgga aaaaatcgac tatgaagcat caacgattga tttagaaggt
    14581 cagctctatg agttgaaagg aacttgtttt caaacgatcc agccagatga tccaaaaaaa
    14641 ttcttggaag aggaacaaaa agtgattgat agtttgatgt attcattcca gcactctttg
    14701 agaatgcaaa aacatatgac gtttttgatc gaaaaagggg gaatgtatct cacgaacaat
    14761 cagcatgtgc tgtttcatgg ctgtatacct gtagacgaga agggacagct cttagcattt
    14821 gaattagacc aatcttatcg cggaaaagaa ctgctgggct tttttgaaaa acagataact
    14881 gaaagcgcaa aagatctgac tgcaaaagaa gatttagcaa ccgatttgat ttggtatgcg
    14941 tggagtgggc cattttcgcc tttattcggc aagagtaaaa tggcgacttt tgaacgttat
    15001 tttctcacag agcctcatac gcacgtcgag aaaagcaatc cttattatca cttgcgagat
    15061 gaagagtggt tcagtgaaaa aattttagca gaattcgaca caaaagaaga agggtcagca
    15121 atcattaatg ggcacacgcc agtaaaagta aaaaaagggg agtcaccgat caaagcaaat
    15181 ggaaaagtat ttgtcattga cggaggttta tccaaagctt atcaaaaaac gacaggaatt
    15241 gccggttatt cactattatg caattcttat ggtttccaaa tcgtgacaca ttatccgttt
    15301 ttgcctattg aacaacaatt tgcaaaaaaa tccgaccaaa caaatgtgaa aaaagtaatc
    15361 gaacagcaac taccacgcaa actcaatcgt gatacaacaa aaggtatgga actgcagcag
    15421 cagattaccc agctcaaaag gctgttggac tatcgaaagg acgattgaga agaattccag
    15481 tgaacattgt tccatagact ggagactgag acaaaagtcg tgtcgcagtc tctttttccg
    15541 gataaacggt gttcttctgc caactcctcg gtcacaagtc acactattca acaaacatga
    15601 aaagctgttt tttgaaattg ctccttgtga ctcagagttg aacggcaggc tcacgacctc
    15661 ttggtataaa cggtgttcaa aagccagttt ctcggtatta agccaaattt atgcaaaaat
    15721 gtgaaaagcc attttcacaa aatttttctt aatactcgaa actaaacagc tttttcacaa
    15781 ccccctcaga aagtctatta aaattcattc acaaagaaat cgaataagcg ctgttcagat
    15841 ggatgtacac ggtgggtaag ttccggatgc cattgaacac cgaggatttt atgcatatca
    15901 tctttgcttt gaaccgcttc aacgagtcca tcgttagaaa aagcgatggc ttttaataaa
    15961 ggtgaaagat ctttgactgc ctgatgatgg taagaattca caaaatatgg atcggggaga
    16021 aggttgctta acaaactgtc tgaaacaatc ttgacttcgt gagtcgtaaa ttgcggggca
    16081 gtgggctgtt gttcatgttt tactgaccac tttggatata atgaaagatc ttgataaagt
    16141 gtaccttcca aaacgacatt caatagttgg agtccccggc agactgcaaa aattggtttt
    16201 ctttgtttca atgcttccaa gatcagtgcc tgttcaaatt ggtctcgttg aatatttgtt
    16261 tccgttagct ttggatgagg atcttccatg taaagttgtg gactgacatc ttgaccacct
    16321 gcaagaataa gtttgtcgat ttgtccaatc agttgaggag ctgaatctgg agacatgact
    16381 ggcagaataa gaggcaatcc ttgagcatct aaaactgcat ccacgaatcc ttgcggtgta
    16441 tagctcactt gatttccttg gaaagtatct gttgctcgaa tcagttgatt tccggcaatg
    16501 ccgatgattg gtttcatttg tattccccct aaagtagact tattgtaaca aggatttaaa
    16561 aaaaattaaa ctatgtaaga cttgccccta tacttatctg ttcttgctgt gttactatct
    16621 gtaaagagaa atttcgtgtg aaattcatga cgatagttga aaatattgct taatttgtta
    16681 taataaaaag taagcagaaa taaaaaggtt gggcgtaagc ttcaaggatc tagttcttca
    16741 aggggtgaga aggacatttt attgaagggg cctgcctata ctaagttatc tttagaggta
    16801 catactttca atgaaaaaag aaagcatgta cctctttttt aaatcgaaag gaagtgaagc
    16861 aagtgttaga agctttggaa caaatccgaa aagcagaaga agaaaatgaa aagcgtgctc
    16921 aaacactgcg gcagcagctg aagatctatg caacagaaaa agagcaggaa ttggaaacga
    16981 tccaaacaaa ctatcgacaa caactaaaac agttggtgga agaaaaagaa caatttgaaa
    17041 tacaagaatt agaaaaagaa aaaatgaaat tgaaagcaga agcagacaag gtgcaagcaa
    17101 ctttgaaaga tcaatatcaa aagcatcaga gtcaagcgat cggtgcgatc attgaaaggg
    17161 tgaaagaaac ttatggccgt cactaagatg gaaaaagtga ccctcatttc agataaaaga
    17221 aatcaggaga ggattttaca agcagtacaa ggaatccaaa acgttgaaat ccgggatctg
    17281 tatcaaaata gaacaaacaa ccagtgggtg gagaagtatt ttccagatac cacgattatt
    17341 gataaagaag caaaattaag cgctttaggt aatcggttga aagacatcca agaagctatc
    17401 caattcatac aacaccacgg cgatagccgt caaaagaaac tccatttgaa acgtcaagaa
    17461 ctttccttac aagatttgga agccaattat tccgaagaaa atttcataaa taaattgaaa
    17521 gaagtccttg atttaaaaga aaagtgggaa caactaactg aagaacgtga tcgtttagaa
    17581 gaagaggaag aatggcttct tcattggcaa cagctggata ttgctccaag tatgtatgca
    17641 agtaagacga ccatttttga gatggggact gtgagtgcag caaattttga aagtttcaag
    17701 gaagaactga gtaagcttga tgacgtttat atggaagaaa tagattatac gccaaaacaa
    17761 gtttatcttt cgtatgttgc tttaaaaaaa tcagttgctg ttatagaaga aattgctggg
    17821 aaatacgggt tcaataaaga aacgtatcct tatgaacaaa atcctaaaga aaaactgcaa
    17881 gagaccaagc agcaattatc cattgttcac gagcagcaaa agaagataac gacagcgtta
    17941 ggtcagtgca gtggctatat ccaagaattt gaatgggtag aagaagtcac acttgccgtt
    18001 gcagaacgag aaaaaataaa agaacggttt atccatgcga cttatctgat cgttttgcaa
    18061 ggttgggtag atgcagaaga aaaacagaat ttgctgcatg tgttaaacga aaccgtatca
    18121 gaagaagata tatacgtcag tttcgaaaca ccaaactcag aagagattca gcaggaagtc
    18181 cctactaaat tgaaaaacca tccgcttgtc gaaccttttg aattgctgac tgaaatgtac
    18241 agtctgccaa aatatgaaga agtagatcca acaccttgga tgacaccatt ttatcttgtc
    18301 ttcttcggga tgatggtcgc agatgttggt tatggtttgc tgatgctcat tgggacaata
    18361 ctagctcaaa aactacttgt tctaccacgc gggatgaaac gtttcgtaaa attctttgag
    18421 attctctcga taccatcgat catctgggga ttgatctaca gttcattttt cggacagtca
    18481 ttaccaaaag aactgtttgg catccgcttg cctttcccaa tcctatctac gacggatgac
    18541 gtcaatacga ttttgatttt atccgtgatt ttcggtctga tacagatttt ggttggatta
    18601 tttgtggcag caaaggaaaa tatcaggaga aaagattact tgagtgctgt cagtgatgga
    18661 tttgcctggc aagggatcct aattgggatc gttattgcgt tggttggtgc catgttgata
    18721 aaaaatacag catttgttta ccttggcgct gggctatcga tcattgctgc cttatgtatc
    18781 ttgatcgttc cgattatcca aacgccgtct aaagtcaaag gtgctgccaa aggtgcgtat
    18841 aatctttacg gactgaccgg ctatatcggt gatttagtca gttatacacg tttgatggca
    18901 ttgggtattt caggcggaag tattggagcg gcattcaata tgctagttgc tttcatgccg
    18961 ccagctgctc gattcagtgt agggatcctt ttgatcatcg ctttacacgc actgaatatg
    19021 tttttgacac tgctaagtgc ctatgtccat ggcgcacgat tgcagtatgt cgaatttttt
    19081 gggaagtttt atacaggagg cggacgtgcc tttgatccgc tgaaaacggc ggaaaaatac
    19141 gtcaacatca atcacaagaa aaaaaacaat aaataattgg aggattttta tataatgatg
    19201 gattacttaa ttagtcaaaa tggtggaatg gtatttgcgg tattagcgat ggcaacagcg
    19261 acgatttttt cagggatcgg ttcagctaaa ggtgttggaa tgacaggtga ggcagcagca
    19321 gcattgacaa caagtcaacc cgaaaaattc gggcaggctc tgattctgca attgcttcca
    19381 ggcacgcaag gtttgtatgg attcgttatt gcctttttga tttttatcaa cttaggaaat
    19441 gacatgtcag ttgtacaagg actgaacttt ctaggtgctt ctctgccgat tgcttttaca
    19501 ggcttgtttt caggtattgc ccaaggaaaa gtcgcagcgg caggtattca aattttagcg
    19561 aaaaaacctg aacatgctac aaaaggaatc atttttgctg cgatggttga aacatacgcg
    19621 attttaggtt tcgttatttc cttcctatta gtgttgaatg cctaagcata aaggaggaaa
    19681 agcagatgaa cgccattgaa aaaatcatct ctcagatgaa cgaagcagca gagcaggaaa
    19741 gagcggcgtt agaacaagaa gagcgaatga agatcgatca aaactttgaa caaaaacgta
    19801 cgcaagttga aacagaacat caaaaacaaa aagaaaaaca aatcgaatta ctcgaaaaaa
    19861 aataccgtca gctaaggaat cgccagcaag tagaagtacg acaagaaaat ttgaatgcga
    19921 agcaggaatt tttaagacga ttgtttgcag atgcggtaac tgaaatggaa aattgggatg
    19981 aatctgaaca aatccagttt atcaaaaatg cattgtattc attacctttg acaggaaaag
    20041 tagcattcat tgctggagaa aaatcagcag cctatctttc tcagacgttg ttggatgaat
    20101 ggaacaatga acttcccttt atgatggttt taagtgatga aactgttgct gatcaagcgg
    20161 gttttctgat aaacgatcaa ggagttcagt ataattttct tttcagcagc cttgtacaag
    20221 atatccaagg aacgatgagt tttgagattg ccaatcaatt attcgaataa ggagggatca
    20281 gtgtatggaa taccacgaac taaatccctt gattcgcgga agagagcttg aacttttgtc
    20341 caatgagaca tttgaacgga tgattcaaac aaattctgtc gaagcacttg gtgaaatatt
    20401 gaaatccacg atttacagtc catatattta tgacggatat gaatatgact tcgaacagaa
    20461 tcttttaaag gagcagagcc gtctatttcg ctggttaaag gaatcagcac cggagcctga
    20521 aatcgtttgg atctatacga tgcgttatac tttccataat ctgaaggttt tgacaaaagc
    20581 tgagatgaca aatcaaaatc tggaccattt gtatatcgac gatggatttt attcattaga
    20641 aaccttgaag gatgccattc atacgcaagt ttcaaatgaa cttcctgaaa gcattatgga
    20701 cagtatacga gaagtacgtg attattttga agagtctacc attcttcagg gaattgacgt
    20761 gatttatgat cgacagtttt tgacagagca gcgacggctt gccgataagc taggatatca
    20821 agaattgctg acagaagtca ttgcttttat tgacttgacc aatatcacaa cgatggctcg
    20881 aggaatattg cagaaaagaa catatgggtt catgacgacg gtactttcca gctcgggaag
    20941 tatcgaaaaa gaagtatttt tgaactttgt agataaagat atggaagatt acacgaattt
    21001 tctattagag acagattatg ctgattttct gcgccctgtc atccgtgagg gaaatattga
    21061 tctcgtaaag cttgaacagt tgaaagatga ttatctaagt tcattgtatc aagaagcaca
    21121 aacccaggct ttcggaccac tgccactact agcctttttg aacgcaaaag aggtagaaag
    21181 caaaaacctt cgtttactgg ttatcggcaa aagaagccat ttcgaatcag aaacaatcag
    21241 aaagcgggtg agacaagtct atgacgcata agatcggtgt agttggcgac aaagattctg
    21301 ttttgccatt caaattgttt ggctttgatg ttcgttatgg tacaaccaag caaaccgttc
    21361 gtcaggcaat tgaagaaatg gcaaagaatg agtatggcgt gatttatgtc acagagcaat
    21421 gtgcagcatt gagcccagaa acgatcgaac gttacaagga acaaatgata cctgctatcg
    21481 tattgattcc caatcatcaa ggatcattag ggatagggct ggaagaaatt caaaaaaacg
    21541 tagaaaaagc agtaggtcaa aatatcttat agtatgaaaa ggagatagaa attttgcaaa
    21601 ttggaaaaat cataaaagtc tccggacctc ttgtaatggc tgagaacatg tctgatgcca
    21661 gtatccaaga catgtgtcta gtgggagatc taggagttat cggagaaatt atcgagatgc
    21721 gtggcgatgt ggcgtctatt caagtatatg aagagacttc tggaattggt ccaggcgaac
    21781 cggttcgctc tactggtgaa gctttatctg ttgaattagg accagggatc atttcacaaa
    21841 tgtttgacgg aatccaacgg ccattagata cctttatgga aatcacacaa agtaatttct
    21901 tagggcgcgg agttcaattg cccgcattgg accatgagaa aaaatggtgg ttcgaaccaa
    21961 cagtagaagc cggagaaacg gtgagtgctg gagatgttat cgggatcgta gaagaaacaa
    22021 aagttataaa acataaaatc atggtgccaa atgggataaa aggaacgatc aaaacaatcg
    22081 aaaaaggttc attcactatt gatgaaacag tatgtgtcat tgaaaccgaa aatggtgaca
    22141 aaaatttgac gatgcttcaa aaatggccag taagacgtag tcggccaatc aaagaaaaat
    22201 taaacccgga tgcacccatg atcactggac agcgagtgat tgatacattc ttcccagtga
    22261 caaaaggagg agcagcagct gtacctggtc cttttggtgc agggaaaaca gtcgttcagc
    22321 atcaaattgc aaaatgggcc gatgtggaca tggttgtcta tgtgggatgt ggcgagcgtg
    22381 gaaatgagat gacggatgtt ttaaatgaat tccctgaatt gattgaccct aatacaggag
    22441 aatcgttgat ggaacgtacg gtattgattg ctaatacatc aaacatgcca gtagcagctc
    22501 gagaagcttc tatctatacg ggaatcacaa ttgcggaata tttccgtgac atgggttatg
    22561 acgttgctat catggcagac tccacttcac gatgggcaga agcacttcgt gagatgagcg
    22621 gacgtttgga agaaatgcct ggtgatgaag gttatccagc ttatcttggt tcacggcttg
    22681 cggaatacta tgaacgatct ggaagagtca ttgcattagg atcagaaggt cgcgaaggaa
    22741 gtataaccgc aatcagtgct gtatccccgt ctggagggga tatttcggaa ccagtcacac
    22801 aaaatacatt gcgggtcgta aaagtattct ggggtcttga ttccagttta gcccaaaaaa
    22861 gacattttcc ttcgatcgat tggatacaaa gctattctct ttattcaaca gaagtaggga
    22921 agtatatgga tcatatcctt caagctgact gggcatccat ggtcaccgag ggcatgcgta
    22981 ttttgcagga agaacagcag ctgaatgaaa tcgtgcgttt ggtagggatt gattcccttt
    23041 cagataatga ccgtctgaca ttagaagtgg caaaatccat tcgtgaagat tatttgcagc
    23101 aaaatgcttt tgatgatgtc gatacattca cttcaagaga gaaacaattc aaaatgttga
    23161 agatgatctt gacctttggt gaagaaggac gccgtgcgtt gtcgttaggt gcttatttaa
    23221 gtgagatcat ggacggtacc gtatcgattc gtgaaaggat cggacgaagc aaatatatcc
    23281 cggaaacaga attagaaaaa ctagatcaaa ttattggtga aatcaagcaa acgatgcaaa
    23341 cgatcgtttc agaaggaggc atgagcaatg attaaagaat atcgtacgat cggtgaagtc
    23401 gtcggtccat tgatggcggt tgaaaaagtt gctggtgtca aatatgaaga attaatcgaa
    23461 gtccgtatgc aaaatggcga gattcgtcgt ggacaagtat tggaagtcca agaagataaa
    23521 gcaatggtcc aaatattcga aggaacaagt ggtatcaatt tacgtgactc ttctgtacga
    23581 tttttaggcc atcctttaga actaggtgta tcggaagaca tgatcggtcg agtattcgat
    23641 ggtttgggac gaccgaaaga taacggacca gagatcctgc ctgaaaagca tttggatatt
    23701 aacggagaag taatcaatcc agtagctcgg gattatccag atgagtttat ccaaacaggt
    23761 atctctgcaa tcgatcattt gaatacatta gttcgtgggc aaaaattacc agtattttct
    23821 ggctctggtc ttcctcataa agaattagct gctcagatcg cgcgacaagc aacggtactc
    23881 gattcatcag atgatttcgc cgtagtcttt gctgctattg gaatcacttt tgaagaagct
    23941 gaatttttca tggaagattt tcgtcaaacg ggcgcaatcg accgttctgt catgtttatg
    24001 aatttagcaa acgaccctgc aatcgaacgt atcgctactc ctcgtatggc tttgactgct
    24061 gcagaatatc ttgcttatga aaagggaatg catgttttgg ttatcatgac agacatgacc
    24121 aactatgcag aggcattgcg agagatttct gcagcacggc gagaagttcc aggaagaaga
    24181 ggatatcctg ggtatcttta cacgaactta gctactttat tcgaacgagc aggaagaatc
    24241 cgcggactaa aaggatcggt tactcaaatt ccgattctaa caatgccaga agacgataaa
    24301 actcatccaa tccctgactt gactggttat attacagaag gacagatcat cttgactaga
    24361 gagctttaca agagtgggat tcagccacca attgatgtac tcccgtctct ttcgcggtta
    24421 aaagataagg gaactggacc cggaaagaca agagaagacc atgcagctac gatgaatcag
    24481 ctttttgcag cctatgcaca aggaaaacag gcaaaagaat tagccgtagt tttaggtgaa
    24541 tctgctttat ctgatgtaga caagatctat gcgaagtttg ctgagcggtt tgaaaaagaa
    24601 tatgtcaatc aaggattcta cacgaaccgt tcgattactg aaacacttga tctagggtgg
    24661 gaactgctat cgatgttgcc aagaaccgaa ttaaaacgaa tcaaagacga catgctggac
    24721 aaatatctgc cagaagggaa gtgagttctg atggcacgat tgaatgtgaa tccgacccgc
    24781 atggaattga ctcgattgaa aaaacagttg acaactgcca caagagggca taaattatta
    24841 aaagataagc aagatgaact gatgcgtcaa ttcatcctgt tgattcgaaa gaacaaccag
    24901 ctgcgtcaag aaatggaaga gtctatgaca gaagcaatgt ctgattttgt attggcaaat
    24961 gcttcgatca cggaaccttt catcgaagaa ttatttgttt tgccggcgga aaacgttgat
    25021 ttgtctatcg tagacaaaaa tatcatgagt gtaaaagtac ctgtgatgaa ttttgaatat
    25081 gatgaacagc tgacagacac acctttggaa tacgggtatt tgaattccag tgccgaactg
    25141 gatcggtcaa tcgatcggtt tactaccctg cttccaaaac ttttggaact gacagaaatc
    25201 gaaaaaactt gtcaactgat ggcgagtgaa atcgaaaaga cacgtcggcg agtcaatgca
    25261 ttagaatata tgacgatccc tcaattagaa gaaacgatct attatattcg tatgaagctg
    25321 gaagaaaacg agcgcgcaga agtaacaagg ttgatcaaag taaaaaatat gggtaccact
    25381 gactaagtgg tatctttttt taaaagagga aaacgatgat taaacaaagc aaacaaataa
    25441 agaaacgttt gccggctgtt cagcttctcg cattaggatt ttttctcctg attttagttg
    25501 gcggcggcct acttacccta ccatttttta gtaatagtgg gcatgggaca aattttatcg
    25561 atgcgctttt cactgcaact tctgctgtct gtgtaacggg attgacaaca ttgaatacag
    25621 cagagcattg gaacagtgct ggtcaatttt tgatcatgct tttgattgag atcggcggtc
    25681 ttggttttat gatggttccg attctatgct ttgcattgat aaagaagaaa gtcagtctca
    25741 gcacacgtat tatattaaaa gaagcgctga atctagaaga agtatcaggt gtgatgaagc
    25801 tcatgcttta catcctaaaa ttggcctttg tgatccaatt gattggtacg gttgctttat
    25861 cttttgtatt tgtgcctgaa tttggctggt tgaaaggtat ctggtacagt ttgttccatg
    25921 caatctctag tttttgtaat gcagggtttg atttgttagg agacagttta gccaatcacc
    25981 agcaaaatgt ttatttgatc atggtggtat ctgctttgat catttctggt gggttaggct
    26041 tcatcgtatg gcgtgattta ctggaatatc atcagaaaaa gaagatgacg attcattcaa
    26101 aaatcgcatt aaccgttaca ggtacccttt tattaggcgg attcgttgtg ttctttttca
    26161 cagagcgcaa tggggcaacg ctagtggaag ggaatatgtt tcaacgattg gcaaacacct
    26221 tttttatgag tgtgacacct agaacggctg gatattattc agttgattac ctccatatga
    26281 gccatgcagg tctgatttta acgatgtttt tgatgtacat cggcggaaca tctgggtcga
    26341 cggccggcgg attgaaaaca actacgttag gtgtgttgct gatccagatg cgttcgatgt
    26401 tgaaaggacg tacgagagcg gaagcatttg gccgtaccat ccgacaaggc gcagtgttgc
    26461 gggcacttac tttatttttt gttacgttga cgttgtgtgt agtcgccatc atgattttat
    26521 cggtgactga aacgatacct aaaacttctg gcatcgaata tattgctttt gaggtcttct
    26581 ctgcgtttgg tacagtaggt cttacgatgg gattgacgcc agacttgact gcaataggga
    26641 aattgatcat tatagcgctt atgtacattg gacgagttgg tgtattgact gtggtcttct
    26701 ctctgatgac aaaagcacaa agaacacaat caaaatacaa atatccagaa gaaagtgtca
    26761 tgattggata atttcatctt ctgacagaaa cctagtgatt tagtaaaata agaaaataaa
    26821 ataatttttc ttgcttcttg gatactttaa gtgcaaaatg ttcgtttatt ttttgaggca
    26881 ctacccgttg ttgaacctcg ggtagtgcct cttttttatt gaaaggaatc aataagattt
//
LOCUS       DAZBSA010000032        26142 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000032 DAZBSA010000000
VERSION     DAZBSA010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.032"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..318
                     /locus_tag="ACHGMT_001215"
     CDS             <1..318
                     /locus_tag="ACHGMT_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN6834974.1"
                     /translation="ANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYS
                     TNLIEGFNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLE
                     SMF"
     gene            791..1345
                     /locus_tag="ACHGMT_001216"
     CDS             791..1345
                     /locus_tag="ACHGMT_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6834975.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(1518..1955)
                     /locus_tag="ACHGMT_001217"
     CDS             complement(1518..1955)
                     /locus_tag="ACHGMT_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6834976.1"
                     /translation="MFSSEENKPLYLDYVNHNLKIIIKENNLKHITLHGFRHTHCSLL
                     FESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFMSSTEEKQGSLQK
                     IKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            2003..2950
                     /locus_tag="ACHGMT_001218"
                     /pseudo
     CDS             2003..2950
                     /locus_tag="ACHGMT_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            3221..3656
                     /locus_tag="ACHGMT_001219"
                     /pseudo
     CDS             3221..3656
                     /locus_tag="ACHGMT_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            3672..4505
                     /locus_tag="ACHGMT_001220"
     CDS             3672..4505
                     /locus_tag="ACHGMT_001220"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN6834977.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            4750..5775
                     /locus_tag="ACHGMT_001221"
     CDS             4750..5775
                     /locus_tag="ACHGMT_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN6834978.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5913..6038
                     /locus_tag="ACHGMT_001222"
     CDS             5913..6038
                     /locus_tag="ACHGMT_001222"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834979.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            6063..7178
                     /locus_tag="ACHGMT_001223"
                     /pseudo
     CDS             6063..7178
                     /locus_tag="ACHGMT_001223"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            7261..8157
                     /locus_tag="ACHGMT_001224"
                     /pseudo
     CDS             7261..8157
                     /locus_tag="ACHGMT_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            8173..8997
                     /locus_tag="ACHGMT_001225"
     CDS             8173..8997
                     /locus_tag="ACHGMT_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6834980.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            9029..10339
                     /locus_tag="ACHGMT_001226"
     CDS             9029..10339
                     /locus_tag="ACHGMT_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFN6834981.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            10487..11746
                     /locus_tag="ACHGMT_001227"
     CDS             10487..11746
                     /locus_tag="ACHGMT_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFN6834982.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11756..12628
                     /locus_tag="ACHGMT_001228"
     CDS             11756..12628
                     /locus_tag="ACHGMT_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6834983.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            12640..13470
                     /locus_tag="ACHGMT_001229"
     CDS             12640..13470
                     /locus_tag="ACHGMT_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6834984.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            13510..15693
                     /locus_tag="ACHGMT_001230"
     CDS             13510..15693
                     /locus_tag="ACHGMT_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFN6834985.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15754..16974
                     /locus_tag="ACHGMT_001231"
     CDS             15754..16974
                     /locus_tag="ACHGMT_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFN6834986.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16971..18431
                     /gene="gtfA"
                     /locus_tag="ACHGMT_001232"
     CDS             16971..18431
                     /gene="gtfA"
                     /locus_tag="ACHGMT_001232"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFN6834987.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            18508..19614
                     /locus_tag="ACHGMT_001233"
                     /pseudo
     CDS             18508..19614
                     /locus_tag="ACHGMT_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19936..20145
                     /locus_tag="ACHGMT_001234"
     CDS             19936..20145
                     /locus_tag="ACHGMT_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFN6834988.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMASKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            20138..20287
                     /locus_tag="ACHGMT_001235"
     CDS             20138..20287
                     /locus_tag="ACHGMT_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFN6834989.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            20441..21106
                     /locus_tag="ACHGMT_001236"
     CDS             20441..21106
                     /locus_tag="ACHGMT_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6834990.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(21390..22475)
                     /gene="xerS"
                     /locus_tag="ACHGMT_001237"
     CDS             complement(21390..22475)
                     /gene="xerS"
                     /locus_tag="ACHGMT_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFN6834991.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22809..23042
                     /locus_tag="ACHGMT_001238"
     CDS             22809..23042
                     /locus_tag="ACHGMT_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFN6834992.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            23039..23446
                     /locus_tag="ACHGMT_001239"
     CDS             23039..23446
                     /locus_tag="ACHGMT_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFN6834993.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(24583..25101)
                     /locus_tag="ACHGMT_001240"
     CDS             complement(24583..25101)
                     /locus_tag="ACHGMT_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFN6834994.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            25468..25716
                     /locus_tag="ACHGMT_001241"
     CDS             25468..25716
                     /locus_tag="ACHGMT_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834995.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         8926 a         3985 c         4844 g         8387 t
ORIGIN      
        1 gcaaataatc aaataaggtt tatgatagat aaatggaaaa agcagtatcc acgagtggtt
       61 aaattgctca tgaatcctgc tatattaacc ttttataatt tccctccgtc aatcagaaga
      121 actatttatt caactaattt gattgaaggt tttaacaaac aattaaagaa atataccaaa
      181 agaaaagagc aatttcctaa tgaagaatct ctggagagat ttctagtatc tcaattcaac
      241 aactataatc aaaagtttct atgccgagtt cataaaggtt ttaaagaaat acatgataca
      301 ttagaatcaa tgttttaact tgatcaacag aatgggcttt tccatttaca caaaattatt
      361 gacgctcccg acaaaattat gatcttgcgt aaagttataa aatattaata aaaatatttt
      421 attaatgaaa agcttggtag cacggttatt catatgctaa tttatttcac tttatagtaa
      481 ggggagttct tagtatattg ttcaaactta aatttcatat aatttaatta tgtaaattag
      541 ctattctaag aacttctacg gataactgcc cgtctaaaat ttaaataaga gcttagaatc
      601 ttcatataag taaggttaat ttttttgtac aaaaaagtcg gaatattctg agttactgaa
      661 atgatataaa atcataaact aaatcccttg attttattga ctttcaaaag tttatatata
      721 taagttaatg attatggtaa aatatataca taatcattaa ctcagaatat ttttaagaag
      781 ggaaacaata gtgagctata atgtacaacc gttaaggaca aatgaagaga tacaagattt
      841 tcagttttgg ctaagaagaa caagtaatcc ggaaagagat agttttttat ttttgtttgg
      901 aatcaataat gggttacgta tgtcagatat tataggatta aaagttggag atatacgagg
      961 caaaagtaag cctattattg ttgaacataa aacaggtaag cgaaaaccga tttttataga
     1021 taatttgaga gaagaaattt tattatatac tgaaggtaaa gaagaaaatg attggttatt
     1081 tccgagtcgg caacaaggtc gacatataac aagggatcgt gtctatcaga tatatgcaga
     1141 tatagcagaa aaattaggac gagatgatat aggaacacat acattaagaa aaacttttgg
     1201 gtatcactac tacaagaaaa ctagagacat tgctacctta atgtttattt ttaatcatag
     1261 ctcgcaagca attacaaaaa ggtacattgg tataactgaa gatgaaatag gagcatcact
     1321 tcgtggattt aaactaggtg tataatttaa aaatttttat aaacagtaaa gacaaagatt
     1381 aaaagctatt tttatctaga acgattttat ttcataaagt tcgcggaata aaagctgggg
     1441 ttactggtac ttggtatagg ttcgtgttat ataaactaag taattaaata acatttttag
     1501 gttggaaaag gatgaaatta aaaaaagaga atcaatacgg tctcaaaaac gcaaacgctg
     1561 cggaataaga aaaatactga taactcaacg tttagcagac tatctaatag ggatagtctg
     1621 cttttttcgt ttttgaccat acttttgatc ttctgcaaac ttccttgctt ttcctctgta
     1681 gaactcatga aatctgcaaa acgaattgcc gtttcctctg tctgcttttt cgcgatatgg
     1741 gtgtatatat catccatagt ggttttaata tctgtatgtc caagtcgaac atacacttct
     1801 ttgacagacg caccagattc aaagagcaga ctacaatggg tatgacgaaa accatgaagt
     1861 gtgatatgtt ttaagttatt ctctttaatg atgattttca aattgtgatt tacatagtct
     1921 aaataaaggg gcttattttc ttctgatgaa aacaaaagtt gttcaccatc ttttataggt
     1981 ttgattctca tttttagata ttatgcccgc acgtattcat cacatttcta ttgtgaatcg
     2041 ctttattcga ccgacatttg acttttatca taatatactc ggtttaaaat tactaatgaa
     2101 aacaattaat caagacgatc ataccatgta tcacctgttt ttttcagata atcaccaccg
     2161 tgtgggaact gaactgactt tttttgaagt acaagaaggg aacaaccagt tttttggaac
     2221 caatacgatt gaacgaacga tactaaaagt tccttccgaa gcatcattgc atttttggga
     2281 aatatatttt gaaacacaag gcgtttgcca ttatggaatc gaatcatttt ctggacgacc
     2341 tatcttacgt tttgaagggc cagatgcaac acgattagct ttagttccct tacgcgaatt
     2401 tgaagatatt gatgattatt tcccgtatgt cacagaccag atccctacag agcatgccat
     2461 tttaggaatt gatgccattc aactacgcgt acaatattcc gatgcgactg aacgagagtt
     2521 attgtcggta ggctggtagc acaaagaata cgtcagtttc ttttcgggtc atcaacgagt
     2581 gacaatctta gaaaatcaag acactcagtt ctatcaagaa gttcagatta ttcaagatcg
     2641 tgagaatcca gttgccattg aagggattgg tagcgtccat catgttgcgt ttggcgtaga
     2701 aaataaatct gatttacaac gcatcgacaa acaattacaa gagcgcaatt ttataaattc
     2761 aggaataaaa gatcgtgaat tttttgtatc actttattat cgtgacccaa atcaattatt
     2821 gattgaaatt gctacagggg aaggaaacct agatgcaaaa gcgtatgaaa atcaaagtcc
     2881 acgctttgaa gaaattcctt tatttttacc gcagtactta aatttcattc gtgaacaagt
     2941 ggagcaataa ttagctaatc aaatccatga cgaagagtaa gaggttgact cataagtcaa
     3001 aaaataaaga aaaacgcatc aaatccattc taaagaatgt tgatttgatg cggttcctat
     3061 ttttataaac tatcattttc gactaataaa actacttatg agtcagcctc tctttttttc
     3121 ttgattttta tacctctttc taggtaaact aaacatctgc caaaaatacc gtgtctcatt
     3181 gaaacattct caactatttc aaaataaagg ggagtaccaa atgaacgagt atattgaaat
     3241 tatccacgca agagaaacaa cttaaaaatg tcagtctcaa aattcccaaa aataaaatta
     3301 gtatttttac cagcgtatct ggttcaggaa aatcatcgat tgtttttgat actattaccc
     3361 aagaagctgg tcgtcaactg aatgacacct atgatagttt tacccgttta tttttaccta
     3421 aatacagtcg tccagaggtt gatgagattg ataatctttc tacggcgatt gtggtcaatc
     3481 aaaagaagat tggtggcagt gcgcgctcaa cgttagggac gattactaac atcaatgcgt
     3541 tatttcgtgt gttgttttca tgttttgcaa cacctagttt aggatatgca aatgcttatt
     3601 catttaatga tcctagtgga atgtgttctg tctgtcaggg aatcggtaag acgattgcat
     3661 ttttaatagg attgctttat ttttcggcta ttgataatgc tgattttgta ttttctgatc
     3721 catttaccgc agaagttttg tctggaattg aggaacaggc aaagaatcat ggttattttg
     3781 tattggtgca taatgttaat tcagcagaag atgttaaaac tattcagaga aattggcgtt
     3841 ttgaggggtt cattgttgtt ggtgtacgtg taaaagaatt tttggaatta aataacgttt
     3901 ttgatggtcc tgttgtttat attgatacgc atctttcaaa agagacatta agagaacttg
     3961 aacaacaaat gaatcgttta tttgtaaata caaatgatta ccaagctagt acattggcag
     4021 tggaacattt gattcattat ggtcatgaga aaatagcgtt tctttctttt gactacattg
     4081 taggtgaacc aagtgttatt caagaacgac atcaagctta tatcgataca ttaaagaaat
     4141 atcatttaac ctttaaatcg cattggatat ataacaactc tgaatttgag cgaatttatc
     4201 aagaattagc tgaatttact gcaattgttg ttactggaga ttatttagcg gctaaattag
     4261 taaaattttt aaaaaataag aataaacgag tgatggaaca attatcgatt attggatttg
     4321 atgatattac ttttgcagaa ttaatggatc ctgcgttgac aacaatacga ctaaatccag
     4381 gtaacaaggg ggcgattgct tttcaccaac tgattggact ggccaaacaa gaagaaataa
     4441 aaaatcaagt actactatta gagggagaat tgatttctcg agactctgtt cacaaaatca
     4501 aataaaaaag ctctgtgaaa taagtatagt taacaataaa atatccgaac gatagagaga
     4561 atgaactgtg atctagcagt tcctcgtcgc aaattcacta gaattagcat tatttactct
     4621 aaattgttcg gatatttttt cttgttttta attatgatta cagtgcctat ttttaacgaa
     4681 atttgcttat tttcaaaatg tatgattata tgttagtaaa cactttcaaa tatgaaaaga
     4741 ggagctatta tggtaggaat tagggatgtt gcaaaacggg cgggtgtgtc cattgcaaca
     4801 gtatcgcgcg tgctaaatga ggatccgaca ctttcagtaa ccgatgaaac caaacgaaag
     4861 attgcagaat cggttaattt ttatcattat gtcaaaaaaa gtcccgttgc aaaaaattta
     4921 acaaatattg cattaataac gacagtttct gaagtagatg agttggaaga tccctatttt
     4981 cgaacgattc gcagagggat tcaagtcgaa gcagaacaaa gcaaagtcaa tttgaagaaa
     5041 attattcgtt tgtcagaaac accgttggag caagacgatt tgctaaaatg tcaaggtgtg
     5101 ttgattattg ggcaggtgct atcttctgtg attgaagaag tatcagcaat taattcgaat
     5161 gtcgtagtga ttgatgaccc tagtattgaa gctgaagtag atgcagttta tactgattta
     5221 acaaaagcaa cgaatcagca tttagatcga ttgtataaaa aaggacaccg aaacattgcg
     5281 tttatcggag gaggacgtgt tcgtttagat agccaaggaa ataaacacgt gtcagatgat
     5341 gattatcgaa aaatagccta tgagacgtgg atgaaacaaa aaggattaga aaaatatagt
     5401 caaatttatt tagaaggctg gaccacactt gacggaatgc aggcagcaga tttacttttg
     5461 aaaaaacatg ctaaagataa gttgccgact gctatcatgg taggaaatga tcctattgct
     5521 gtgggggttt atcgttcact tcaaaaaaat ggtctacata ttccagaaga tatttcgatt
     5581 gtgagttttg ataatattga agttgctgaa tttttaacac ctgcattaaa tacggtgaat
     5641 gtgaatacag aagaaattgg aaaactagca gttcgcatgg ttaatgaacg aataaaagaa
     5701 gttagaaatg tttcgatcaa agtgattgtt tctaataata ttattgttag agaaagtgaa
     5761 aaaaatatcg attaaaaaag taaacatcca aaaaagggtg tttacttttt tgtttactaa
     5821 tatttttagt aaaaaatctc gacaaaacat taagtaaaca gttatggtgt tgttaacaaa
     5881 aagaaaacgc attcaaaaca ggaggaatta agatgaaaca tttcaaaaaa atagctttaa
     5941 cagggatagc tgttttaact gggacattac tcgcagcatg tggtggggga gatagtaatt
     6001 ctaaagacgg gcaagtaact ttaagctatg cgagttgaga tactgaccaa gctccaggac
     6061 taagaaaaat gttagatgaa tttgaaaaac aaaatcctga tatcaaagta gaaatggaga
     6121 caacgccttg ggatcaatat tgggttaaat tagaagctgc aacaacggga ggtaatatgc
     6181 cagatgtagt aaccatgcac tctagcgaat cctataaata tatgtcacag aatgctttga
     6241 tgggacttga tgatgttatc aaagaaaatg atattgacat gggaaacttt actgaaggaa
     6301 ttgctgattt ctatacgctt gaagacgaac tatacgcaat ccctaaagat gcttctgtag
     6361 tggggctatg gtacaacaaa gaattatttg ataatgcagg tgtcgattat ccagatgaaa
     6421 catggacatg ggaaacactc agacaagctg caattgattt aacagatgaa tcaaaaggca
     6481 tctatggttt tgcatcacat attggaacag agactggcta ctggccattt atttatcaaa
     6541 atgggggaga agtatttgcc gatgacaata caaaatcagg tttgaatacc ccagaagcac
     6601 aagaagcttt agctttttat cgagatattg cacttgttga tcatgcttca ccaacaatta
     6661 cagaattaca agatcctgat aagttaagtc gttttcaatc aggtagatta gcaatgatga
     6721 ttgaaggaaa ctggtttgca ccgggatttt tgaaaaatga ctatctagtt gaaaatgttg
     6781 cttctgccgt gttacctaag ggaaaagtta atgcaacaat tacaaatgga cttggttggg
     6841 ctgccagtgc gagtacaaaa tacccagaag aagtgaaaaa actaatggca tttttagcta
     6901 gtgaggacgc caataaaatc caagcggaaa cgggggcatc aatcccagca ctaaaaggct
     6961 ttgaagacga gtgggctgct tcttcagata caattgattt gagtgctttt ggaaaaatgt
     7021 tggagtatgg agtccctcgt ccatacaata aagatggatt acgagctgaa caagcagaag
     7081 ttcaaattat taaccgtatt ctgtctggtg aagtaactgt tgaagatgga accgctcaaa
     7141 ttgaaaaaga agtcaatgcg atattgagcg gaaattaaat tagttaatta tacaatatac
     7201 aggcgaaagg attctatttc gtctgtatat taatggatga ataccaaaaa gggggatatt
     7261 atgaaaaatc gaatttttgg aaaatatcgt aaagatcaag tgatatgggg ctatattttt
     7321 atttttccaa cggcattagg attaatttta ttaaatatat ggccagcaat tcaaacagca
     7381 attttaagtt ttcaaaaaac ggtagggttc ggtgatactg aatgggtcgg attaagaaat
     7441 taccaaaaaa tgtttgcaga cggtgaagtt tttcagtctt tattaaacac attaatttat
     7501 gcagtggttt cgatacctag tattgttgta ttatccttgt tggtggcagt tttaatgaat
     7561 agaaaagtta aaggattgtc gatttatcgg acaatttatt ttctaccagt cgttgctgca
     7621 ccatccgcag tcgcaatgat ttggcgctgg atgttcaata atgattatgg actgattaat
     7681 aacatgttgg ctaaaatagg tctcgatgga ccagcatggt taacggatcc atcgattgcg
     7741 atttattcta ttatcattgt aggagtttgg agtgctattg gttataacat ggtgttatta
     7801 ctagccggct tataagaaat tcctaaagat tattatgaag cagcggaaat tgatggtgct
     7861 agtccaatcc agcagttttt caacattacg ttaccattag ttacaccaac attatttttt
     7921 gtagttgtaa caacagttat caatgctttc caagtatttg atgttatttt tatgatgatt
     7981 actcctaata gtaccgccat gtttaaatca caatcattag cctatttatt ctataaacat
     8041 tcatttattt taaatgacaa gggatatggt tcggcaatcg ttatgttcct tttagtgatt
     8101 atattaatta ttactgcaat tcaaatgaag ttgcagaaaa agtgggtaaa ctattaagga
     8161 ggctatcaaa ttatgaaaaa taaatttttt tcaaatgcgc caatccatat catgcttatt
     8221 ttaggtggta ttgcaatggc attacctttt atttggatga ttttaacttc agggaaaacg
     8281 ttggttgaat ctacccaaat tccacctaca attttccctg agaaatttca gctaagtaac
     8341 tatcaacaag tacttgattt attaccattc atggcttttt atatcaatac atttttaatg
     8401 gttgttggac gagtgattgg ctcggtttta ttttcagcaa tggctgcata tgcttgtgcg
     8461 cgtctaagtt ttccagggaa aaatatcctt ttcagcttag tacttattca aatgatgatt
     8521 cccggttcca tttttattac tccacagtat ttattagcac aaaaattggg tattttaaat
     8581 acaattccag cattaatatt accaggggta gttagtgcgt ttggtacgtt tttattaaga
     8641 caatttttct taggtttacc tgatgaattg gaggaagcgg cctatttaga aggtgccaat
     8701 atttggacga tctttttcaa aattatgttg ccgcttgcta aatcaggatt ggttgcactt
     8761 ggaatattca cagcgttgtt tgcatttaaa gatttaatgt ggccattgat tgtcaatact
     8821 tcaattgata agatgccttt agctccggga ttagcaaact tacaaggaca atatgccacg
     8881 aacttcccac aattaatggc aggttcgtta ttagcaattt ggccaatagt tgtgatattc
     8941 atcgttttcc aaagaaaatt cattgaagga attgctacaa caggtagtaa attataaaac
     9001 tattaattag tgattggaga gtaagtacat gacaaaaatt acatttattg gtgcaggaag
     9061 tactattttt gcaaaaaacg tattaggcga tgcgatgtta actccaagtc tacaagatgc
     9121 acatattgca ttgtacgata ttgatgaaaa acgactaaaa gaatcaaatt taatgcttaa
     9181 aaatattaat aaaaattcga atgaaaatcg tgctgatatt gtttcttatt atgatcgcaa
     9241 agaagcgtta agagaggctg attttgttat caatgctatt caagtgggtg gttataaacc
     9301 ttcaacagtg attgattttg atattcctaa aaaatatggg ttgcgacaaa ctattggtga
     9361 caccatgggg attggtggga tttttagaac cctacggaca ctaccagtaa tgttagactt
     9421 tgctaaagaa atggaagaag tttgtccaaa tgcttggtta ttgaactata caaatccgat
     9481 ggcgatgtta accatgggtg ttttaaaagc gacgagtatt aagactgttg gtttatgtca
     9541 tagcgttcaa gtatgcgtac cagaattgtt tgagcattta ggaatagccg atcaatatga
     9601 cttaaatgac tttcaatgga aaattgcagg cattaatcat atggcattct tgttggaaat
     9661 tacaaaaaat ggggaagatt tctatccaac aattcgtcag ttagctgcag aaaaaccaaa
     9721 tccacatcca gattctgtac gttttgaatt gttgaaacgt tttggttact atattacaga
     9781 atcaagtgaa cacaatgcag aataccatcc atactttatt aaatcaaaat acccagaatt
     9841 gattgacgaa ttagggattc cgttggatga atacttacgt cgttgtgaac aacaaattaa
     9901 tgattgggaa agcatgcgag aggacattgt aaataatggg gagttaacac atacgagaag
     9961 ccgtgaatat gcttcatata tcatggattc tattacgaca ggaactccgt cagtgattgc
    10021 aggaaatgtc ttaaataaag gcttaatcac aaacttaccg acagatgttt gtgtggaagt
    10081 accttgttta gtcgataaaa atggcgtgca accaacctat gtcggtgaat tgccaacaca
    10141 attagctgct ttaaatcgga cgaatatcaa tgttcaagaa ctgacagtag aagccgcttt
    10201 aacactagat aaatcaaaaa tttatcaagc agcgtattta gatccacatc tatcatctga
    10261 attgtcttta acagatatca aaaatttagt ggatgattta atcgaagcac atggggacta
    10321 tttacctgaa tataattaaa aagaagctga aagagtaagg aattttcaat tccttactct
    10381 tttcttagtt aaatttagta aaaatattga caaaacactt agtaaacagt tacattagtg
    10441 ttaacaaaaa gaaaacgcat tcaaattgta taaggaggag cataaaatga agtggtctaa
    10501 aaaaattatt ttagcttgta tagcactagg agcaacgatc tctttgactg cttgtggaaa
    10561 aaatcaggaa gatgcaagcg gtaaggtaga aatagagttt tttaatcaaa aaaaagaaat
    10621 gactcaaacg attcaggaga ttgctaaaga ttttgaagca aaaaaccctg atattcatgt
    10681 aaaagttgtg gatgtaccga atgctggtga agtaattaaa acacgtatgc ttgcaggcga
    10741 tgtaccagat gtcattaatc tttatcctca aagtatagaa ttgaaagaat gggcgaaagc
    10801 tggttattta gaggacttaa ccgaagaacc gtatttagag aatatcaaaa atggatatgc
    10861 acaacgtttt gctattgagg atcgtgtata tagcatacca ttaaccgcta atgtctatgg
    10921 tttttactat aacaaaactg cttttgagga aatggggatt caagcaccag aaacttgggc
    10981 agaatttgaa aaaatagtag ctgagattaa agatcaaaac aaagtaccgt ttgcgattgc
    11041 aggagcagaa ggctggactt taaatggtta tcaccaacta gctatagcga ctgctgctgg
    11101 tggtgaagag gaagcgaaca atgtttggcg tttctcagaa gttaatggaa ttaatgctga
    11161 aagcaaagaa atgcaaaaag attttaatcg attagatttg ttaagagaac caaaagcttt
    11221 gcaaaacaac tggcaaggtg ccggttataa cgatacagta gttactttta caaaaggtga
    11281 tgcgttgatt atgccgaatg gttcatgggc aatgccgatg attcattcac aaaatccaga
    11341 ttttgaagta ggaacttttc cttttccagc agatgaagca ggtcaaagtc taacgattgg
    11401 cgcaggagac ctagccttat cagtttctgc aacttctcaa cataaagaag aagccaaacg
    11461 ttttgtagaa tatatgacaa cacctgaagc gatgcagaaa tattatgatg tagatggtgc
    11521 accgtgtgct gttgagggcg ttatcgagaa tactcaggat tcaccattaa gtggtttaac
    11581 tgaacttgct tttactgatc gtcatttagt ttggttagcc aaagattgga atagtgaaaa
    11641 tgatttttat accttaacaa caaattattt acacaatggc aatcaaaaag ccatgattaa
    11701 cgcattaaat gctttcttta atccaatgaa agctgacgga aggtaggaaa ataaaatgaa
    11761 aataaaagaa attttaaatc gttactgggc aatcattttt gtagcaattc caattatttt
    11821 gcagatgatt tttttctact ttccaatggt tcaaggtgct ttttacagtt taactaattg
    11881 gactggtctg acttataatt ttgattttgt gggtttgaac aattataaga ttcttttttc
    11941 tgatccgaag tttatcaaat cgattggctt cactttaatt gtgacgcttt gcatgattgc
    12001 tggacaaatt ggttttggta ttttgattgc gcgggcgttg aacgcaaata ttaaagggaa
    12061 aacattcttt agagcatggt tcttttttcc agcggtctta tcgggcgtgg ttgtttcttt
    12121 agtatttaaa caaattttta actatggttt accaaaaatt ggtagtatgt taggcattga
    12181 atttttacaa accagtcttt taggaacaac atggggagcg atttttgcag taatttttgt
    12241 tttactttgg caaggaattg ccatgccagt catcattttc ttagcgggtt ttcaaagtat
    12301 tccacaagaa attattgaag ctgctagaat ggatggggcg aatgataagc aaattttttg
    12361 gaatattgaa atcacctact tattaccaag tatttcaatg gtatttatta tggcattaaa
    12421 aggtggctta acagcatttg atcaaatttt tgctttaact ggtggtggac caagcgatgc
    12481 cacaacatct ttggggttac ttgtttataa ttatgctttt aaaaacaatc agtttggtta
    12541 tgcgaatgct attgctttag tcttatttat cttaattggg attgtttcac tggcacaatt
    12601 aaaattatcg aaaaaatttg aaatctagga ggtttcccaa tgaaaacatc aaaagaaaaa
    12661 aatatttcta aatacttgct tttattatta gggggcttca tgattttagt tcccttagtt
    12721 tcaacgctat tcggttcatt taaaacaaca cccgatatta tgaataattt ctttcgtttt
    12781 cctaatccag cgactttgga aaatttccga agattgttag cggatggaat tggtcattac
    12841 ttttggaatt cggcaatcat cacaatcatt gcagtagttg ctgtgacagc aattattcct
    12901 atggctgctt attcactagc aagaaaaatg actcgtggga aagcatacgg attgatgtat
    12961 ttatttttaa ttttaggaat ttttgttcct ttccaagtaa ttatgattcc gattaccgtt
    13021 atgatgagta aaattggttt agcaaatatg tggggattaa tcttactcta tctcacttat
    13081 gccgtaccac agacactatt tttatatgtt ggttttatta aactaaatgt gcctatcagt
    13141 ctggatgagg ctgctgaaat tgatggggct agtcacttta caacttattt caaaattgtt
    13201 ttcccaatgt taaaaccaat gcatgcgacg actattattc ttaatgctct atggttttgg
    13261 aatgatttca tgctaccatt attaatttta aatcgtgatt ctaatatgtg gacactacca
    13321 ttgtttcaat ataattatac gggacaatat tttaacgact atggtccaag ttttgcttcc
    13381 tatacagtag gaattgtcac gattacacta gtttatcttg ctttccaaag aagtattata
    13441 gctggaatga gcaatggagc agtgaaataa ccaataaatt attctcatta taaaggagtt
    13501 cataaactta tgattatatt tgacaatcac acacaaacat ttcatttaca aaataaaaaa
    13561 attagctatc tattaggaat cgaagaaaat aattatttaa cgcatcttta ttttggaaaa
    13621 aagattacgc actattctgg tgggtatcgc tacccccgaa cagatcgttc cttctcaccc
    13681 aacaatgcaa atgcgcaaga tcgtttatta tctttagaca cattaccatt agaatttcca
    13741 ggatatggac atggtgattt tagagaaccg gcgcataata ttaaattagg aaacggtagt
    13801 cgtgtaaatg attttcgtta tgatagctac gaaattcaaa aaggaaagcc cagcttagcg
    13861 ggattgccag ccttatatac ggaagatgat gatgaagctg aaacgctgat tattcaattg
    13921 aaagacgcga ttagtggatt gactttacag ttgttctaca caatttatca aaatcacagt
    13981 gtaattattc gttctacgca attagtaaat aatagtgatg aaacagtgga gattaaccgc
    14041 ttagctagtc aatcaattga ttttccaaat cgtccactcg agttaattca tttaaatggt
    14101 acctgggcaa gagaacgtca aatgactaga gaaaaaattc atacagggat aaaagtatta
    14161 gatagtaaac gcggatcaag cagccatcaa caaaatccgt ttgttgcttt agtagatgcc
    14221 aaaacagatg aatttcaagg agaggcatat ggatttaatt tagtttatag cggtaatcac
    14281 gaaacggtga ttcaaaaaga tccatttgac caaacacggg tcattatggg aattaattca
    14341 tttaattttg gttggcaact aaaaactggc gactcatttc aaacgccaga agtcgtgatg
    14401 gtttatagta atgaaggtct gaatacgtta tcacagacgt atcatgaatt gtataatcac
    14461 cgtttaattc gtggaaacta tcgtttaaaa gaacgtccca cgttaatcaa caactgggaa
    14521 gcaacgtatt ttgattttaa cgaagaaaaa atcatgaata ttgtcgatga tgcgaagaac
    14581 ttgggcattg aaatgtttgt tttagatgat ggttggtttg gcggtcgtga aaatgacttc
    14641 acttcattag gtgattgggt tgaaacagaa ggcaaacttg cttgtggttt ggaaaattta
    14701 gccaagcaag tgcatgataa cgggatgaaa tttgggattt ggtttgaacc ggaaatgatt
    14761 tctgaagata gtgaactatt tcgagcacat ccagattggg cattgggtat tccaggtcgt
    14821 gaccgctcaa ttagtcgaaa tcaatatgtt cttgattttt caagacaaga tgttcgtgat
    14881 aatatctatc aacaaatgac ggcgattttt gatcgtgtac cgattgatta tgtgaagtgg
    14941 gatatgaatc gaaatatgac agaagtctat tctgtcttgt tggactcaac gatgcaaggt
    15001 gaagtggctc atcgttatat gttagggtta tatgaatttt tagaaaagtt aacaacgaat
    15061 tatccaacta ttttatttga aagttgttcg ggtggaggcg gtcgttttga tgcaggtttc
    15121 ttgtattaca tgccacaaac ttggacgagc gacaataccg atgccgtggc tcgtctgaaa
    15181 attcaatatg gaaccagctt agtgtatcca atttcaagta tgggtgcgca tgtttcagca
    15241 gtcccaaatc atcaaacagg aagagaaacg agtttaacta ttcgtggcaa tgctgcgatg
    15301 gctggtgttt ttggttatga gctagatcta acgcaattaa cttctgaaga aaaagcagaa
    15361 attaaagaac aagttgcctt ttataaaaag catcgccaac ttttgcaata tggaactttt
    15421 tatcgcttgg aaagtccgtt tgaaggcaat gatactgcat ggatgtttgt ctcaaaagac
    15481 caaacagaag ccatggtctt ttatttccga gtattagcag aagctgcgca tccattagtc
    15541 acattgaaat tagcaggatt agcagaagaa gcaacttatc aaatggaaga tgtaacattt
    15601 gctggcgatg aattgatgaa tctcggtttt tatattgact cagaactaca tggtgattat
    15661 gcgacacaac gcttttattt gaaacagata tagtagcttg aatttacaat ataagtgcaa
    15721 caccaaaaga gactcattcc aaacgaagaa aatatgtttt atttacacgc atttactaaa
    15781 aagcagcctg atttgaattg ggaaaatgaa gaagtacgtg aagaaatcta tgcaatgatt
    15841 aactattggt tagataaagg actaggcgga tttagaatcg atgcgatatt aaacatcaaa
    15901 aaaaaactag agtatggcat ctttgaatct gatggagaag atgggttagc cttcattggg
    15961 ccctggattc ttaatcagtc gggcattgaa gtgtggttaa aagaaatgag agagcggaca
    16021 tttaaaccac ataatagtat gacggtagca gaagccgatg ttcctaatga acgtttagat
    16081 gaatatattg gtgaagatgg ctattattcg atggtttttg attttagtta tacggatatt
    16141 gatgtacctg aaactggtga atggtttaaa gatagccaat ggacatggac ggatatgcgt
    16201 accaatattt ttacgaatca attagttacg caagataaag gttggggcgc cttatattta
    16261 gaaaaccacg atcaaccacg ttcaattaat aaatatattc cagaagaatg gatcaatgat
    16321 tatagtaaaa aaatgttggc gacgcttttt atgttactaa gaggtacccc ttttatttat
    16381 caaggacaag aactaggaat gaccaacata gaaatggcat cattagaaga ttttgatgat
    16441 gtagctacgc attctcagta taaacgggca ttagaatatg gattggcacc agagcaagca
    16501 ttgaaagctg tcaataaacg aagtcgggat aattctcgca cacctatgca atggaatacg
    16561 caaaaaaatg ccagattctc aacttctgat aatgtatggt taaaagtaaa tcctaattat
    16621 ccagaactca atgcagaagc tcagttaacc aatcaacagt cggtattcaa ttattatcat
    16681 caattaatta aattaagaaa aaatagtgta tacaaagacg tgctaatcta cggacaattt
    16741 ctaccagtga aagataaaaa tgaacatatt ttgctttatg aacgccaatt agaagataaa
    16801 atcgtattag ttatcttaaa cgttacagca acagaacaag aatattatgt agatgaacag
    16861 taccaacaag tattaatcaa taattatgaa gatgttttat tagatcaaag tcgaaaattg
    16921 cgtttacgac cgtttgaaag tattgtatta gctaattatg gaggacaatt atgaaaatta
    16981 aaaacgaagc aatgctcatc acctatgcag atagtttagg aaaaaacatg ggcgaattaa
    17041 aacaagtctt agacaaacat ttacaaggtg ttattggtgg tgtacatctg ttgccttttt
    17101 ttccatcgac aggtgatcga ggctttgcgc cgagtgatta tacgacagtt gatccagctc
    17161 ttggaacatg ggaagatgtg gaagcattag gtgaagatta ttatttgatg tttgatttta
    17221 tgattaatca catttctcgt gaatcaaaat ttttccaaga tttcaaaaaa aaccatgagc
    17281 agtcaccata caaagaaatg tttatccgta ttcatgaatt tttcccagaa aatcgaccaa
    17341 cacaagaaga tattgattta atctacaagc gtaaagacaa agcgccgttt caaacggtag
    17401 aatttgcgga tggtacgaca gaagaggttt ggaatacctt tggggaagaa caaattgatt
    17461 tagatgtgac caaagaagtt gtgaaagaat ttattcgaga aaccatcaaa gatatggcga
    17521 accatggctg ttctttaatt cgattagatg cgtttgctta tgcgattaaa aaattagata
    17581 ccaatgattt cttcgtggaa ccagatattt gggaactttt agacgaagtg cgtcaagaag
    17641 cagcgaaata tgacgtcgaa ttattaccag aaatccatga gcattattcc attcaaatga
    17701 aaattgccaa tcatgattat tatatctatg acttcgcctt gccaatggtc actctgtatt
    17761 cactttatag tggaaaatca gaacgtttag ccaattggtt gaaaatgagt ccgatgaagc
    17821 agtttacaac attggatacg catgatggaa ttggtgttgt ggacgctcgt gatttattaa
    17881 cagatgaaga attggattac acctctgagg aattgtataa agtcggggcg aatgtgaaga
    17941 aggtctattc aagtgccaat tacaataatt tagatattta tcaaattaac agcacctatt
    18001 atagtgcatt aggcgataat gaccgtagct atctgttagc acgtgcgatt caagtatttg
    18061 cgccagggat tccacaaatt tattacgttg ggttattagc aggaaaaaat gatattgaac
    18121 tgttggaagc aacgaaagaa gggcgtaata ttaatcgtca ttactatgat ttagccgaaa
    18181 ttgaagaaga agtgcaacgg ccagtgattc aacaattatt tgcactttta aaatttagaa
    18241 atgaatctgc ggcgtttgat ttggatggaa cgattgaagt agcaacgcca tcagaatcag
    18301 aaatagtgat tacacgtaaa aataaaaacg gtagtgatag tgcaacatta acggctaact
    18361 tggatacgaa agaatttatc attgagtcta aagatataaa aattttcact agtgttgcat
    18421 taaaatttta acctattcca tagaacaaaa aatcctttat actagcactt ggatgactta
    18481 tccaagacca ataaaaagga ctcatacatg gataagtata aaacaaatac agcatttaat
    18541 aaatggtttt ctgccataaa attaggaaaa ctttctgtca actcccaaaa atgtattttt
    18601 gattacaatt cgtatcgtaa aaaactgtct tttgaagctg tgctcaagct ctttctctac
    18661 gcagttaatg aagaaaaaga aagtttgcgt gatttgagta cagctcttat cgaccgatcg
    18721 ctccaactag aaaccgatgt gtcatcgatt agtcataccc aattgtctcg agcatttaat
    18781 gcacttgaac ctaaagtgtt agaagaaatt tttcaacaac tgttagaaaa agttcaacat
    18841 caaatcaaac caaccaagcg aaatcatctg tatctgattg attccagtac gttttcgctt
    18901 agtttaaaac gtcataagtg ggcgactttc cgtcaaacaa aatcaggtgt caagttacac
    18961 ttaaactatt gttatatgga cgatactacg atgtatccaa ctgattttac cttaacaaat
    19021 gcccatgaac atgatctcaa tcaactacca gtattggtga atcaaccaga ggcaacttac
    19081 gtgtttgatc gtgggtatta ggactttgaa aagatggatc agatgcattg ggatggctat
    19141 ttcttcgtca ctcggattaa aaaaaacacc aaagttcacg tgatagatac tctggaaaca
    19201 tcgccagaaa cagaaattct acgagacgag ctggttcgtt taggatctaa aacctatcta
    19261 accgccaatt tccgactagt cactgttcaa gataagaacg gacgtgtgtt tcaatttatc
    19321 accaatcgaa tggacgtctc ttcaaaagaa atctctgata tgtatcacgc tcgttggcaa
    19381 atcgaattgt ttttcaagca catcaaacaa cacatgacca ttaaaacctt cttttctcaa
    19441 agtgaaaaag gcgtgcagaa tcaattgatt ttaacgatga tttccgctct cctcacgttt
    19501 ctcatcaaat tggagacgaa aacggaaaaa tcagtgttcc aaatcaaacg attctttcgt
    19561 tatttactgt ttcagccctt tgaatgttgt ctagaaaaat taattcccac atgagaaaaa
    19621 agaaatttgg ctcgttactt ttggttttca gttgaataac agataaaaaa ttttctggaa
    19681 aaagtcatcc tctgcgtcag cacagttttt tttgacttat tttcagagga aatagttttc
    19741 agaatattct aaaatgtttt atgcaacact agtgatattt tttagaatta gatttctaat
    19801 gtgcattttt tgagtattaa ggagcaagta tacaaagatt tataaagctg attaaatttg
    19861 aataaaaata tgcaggaacg ctactatttt tagtagtgtt cttttttata tttgaataaa
    19921 aaatgtggtg gttaaatggt ggttataaca aaaaatgaat taatgaaatt aggttatggg
    19981 aagtatcagt ctgagagaat catacgacag gcaaaagcaa ttatggcttc aaaaagctat
    20041 acctattacg aaaatagaag attaggacta gtacctgttg aatctgtaga agaagtttta
    20101 ggtatgaaga tagaggtaga ggaggatttt cagaatcatg gttaatttta aaaaagataa
    20161 aaatgggaaa tatcatttta ctgctagttt gggattcgac aaacgaacag ggaaaagagt
    20221 acaaaaaaac gtagaggggt taaaacagtt aaagaagaaa aaaagcgtat accgaacttg
    20281 tttctaatta tggaaaagaa gcagaaactt ctcaaagtac tatgttgttt gaggagtttt
    20341 tttataaatt attcctacct tattatcaag ggaaagtaga agaaagaaca tataaaaatc
    20401 ggttatctac aatcaaaaaa acttttctta tttctttaaa atggagttaa gagaaatacg
    20461 tccaattgac attcaaaagt ggcaaaataa tttattgagc aaatataaaa attcttatgt
    20521 aagaaacatg tatgggattt ttcagatgtc attagatcga gcagttgttt tgggactact
    20581 tgaaaaaaat cctgcaaaag ttattggaaa tgtgaagaaa aggaaaacta cagttgattt
    20641 ttggacaaaa gaagaattta caaaagttat ttcaactttt tatattgatg atttgtacca
    20701 acattttggt tttatttgta tgtggttact ttttatgacc gttatgcgtt tgggagaagc
    20761 cactgcatta acttggaaag atattgattt tgaaaataaa aacttgcgtg tgaataaaac
    20821 actctattat aagagagcca aaacctatga gtttgtagaa cccgaaacgg cttctagtat
    20881 tcgtacaatc gaactagacg acaatacaat taccttttta aaagcttgga aggagcgtca
    20941 agtttcaata ggaggcatgt cgtttgtact ttcttataat ggaacaccta ctcagaaata
    21001 tacaatccgt catattatga gaagacatgc aacgatcgtt ggtgttcata taatccgcat
    21061 acacggatta aggcatagtc atgcttcttt gcttatatca caataaaaag atgcacttcg
    21121 aattaaagaa aggttaaaac atgctgatgt acaaacgact ttaggaactt atggtcattt
    21181 gtatctaaat tcattgagag agataacaaa tgatcccaca gattatatcc aaactaattt
    21241 ttctaaaatc aaaccgacac ctatttacga taataaaaaa taaatgataa acaaaagtag
    21301 gatcacacca aaagaaacac actagaaaaa ctataataca ggcttttcta gcgtgttgtt
    21361 tcttatttcc gcctatttat acattatact cataaagcat ttaatgcatc gatcgtagcc
    21421 gcattgtcta tatgggtata taaagttgtt gtattggtac ttgaatgtcc caattgttgg
    21481 gcagttaata ccaatgaatt tgtctgttgg tagagtctag tagctaatgt atgacgcagt
    21541 ttgtgagggc tgacttgaat tctaaagcct tttgaatatt ttgctaccat ccgttcgatt
    21601 gcaataccgg atatacgctt ggcttctcct tggtaaattg ctagaaataa agctttttcg
    21661 ttttctgtta ctttatatcg ttctttccga atagctagat aattagctaa atattccatt
    21721 gcgaatgctg ctatattgac agcatctctt ttatttcctt ttctagttac taccacatta
    21781 ttcttatcta aagataggtc atctaaatta atatttataa cctcggagag tctcaatcca
    21841 gatcctaaaa tcagtgcatt gatcgccagg tctctttcta catctcgttg ataatagatc
    21901 aaagctcgtt tactcaaagt atttttatat tcattggcaa tataatctag ataattttgt
    21961 gtatccttat ctagaaataa ttttttctca atggcattgg cacgtgcagc taacgtagtt
    22021 ttatctgtga tggttgctac ttgatccata ggattattgt ctaagtatgg ttttcctaga
    22081 ggattattgg aacttttcga aagatattta aatagaacct ttaatgccgt gatatttcta
    22141 ttgatagtag aagtagatag acctaagtta ttattatctt tgagcttagt ttttgttgtc
    22201 tcttttttta cattttcttt tgtgcgacgt ttcaaatgag atttgtacaa gtttaggtca
    22261 taggcagtta actgtcttat cgaggtgata gaagtatcag gaacgataga ctcactcaga
    22321 agccattcat aaaagagctt ataatttttt agatattcta aagtggttcg aggtgattga
    22381 tctttatcct cacgatcttg aacaaaatct tttatataat cgggcatatg aagtatgatt
    22441 gtatctattt tttgatatat ttctgtattt ttcatgtcat tcatcctttc aataagtagg
    22501 ggagaagagt atctctataa ttggactata ccaattatag aatgttttgt caattggtat
    22561 gctctaaaat ttattaattt gtttcaaaaa aagcatataa acaggtatac gtttaataat
    22621 ttaaaataaa tggtatacat aaatagggaa ggaaatagaa gggagcaatc taaatttttt
    22681 gaattttctt aattaattat atagaaagaa caatctttct atagagttag acatgagagg
    22741 gactatgctt gatttaaaat caacaagcgt atatactgta tatacaaaga aaaaggaggg
    22801 agaagactat ggcaattaaa gaaagaaaat ttagggaagt ggggaactca gtagtattaa
    22861 ctatttcaaa ggagtttctt aaagaaaatg ggttaaatcc cggagatacg gtttttttag
    22921 atgaagaaaa acttcgagcg gcaattacaa aaaaacaagc cttatctgat gaaattgatt
    22981 tagcaatatc tcaagcatta actgaatatg atactacttt tagagagctt gtcgataaat
    23041 gagtattcat tacctagaag ccaaagatat tataaaaatg aatgtaatcc aaataaaaaa
    23101 atactctcct aatgaacccc ttggtgtaaa ggatgcgaat gctttagaaa tgtgtgtagg
    23161 gcaacttcga gcctcagctt ttggtgaaga ggtttatcct tcgatttatg aaaaagcagc
    23221 tatcctactt atacaattga taaagaaaca tccttttcac aatgcaaata aacgtactgc
    23281 tttcttagct acttttgtct ttttgaaaat caatggacag cttttagcta ttgatcaaaa
    23341 agatgctgta aatttagcgg tatatattgc tacgtatgat aaggatttcg atcaactaaa
    23401 atatgaagtg atcaacacta taaagacaca tacgcagccc atttaaggag ttactgtatg
    23461 tgtctttata gggttattag ctgctatagc gaaataattg aagtaaatta caaatagcta
    23521 taattcaaat ccaactatat agaaaacgta aattttctat atagttggat taagataacg
    23581 actatttatt ttatcatatt tttactatac cctatacttt tacttggtat atttatgaat
    23641 aatcgcaaat ttacccctat actactagtt tgtaagttag attaataaaa atactaaagt
    23701 tatttgttct tttattaaat acacctacaa cttataaatt gatggtattt agataacacg
    23761 aaagaataaa aataacagct gatagtaaag tttgttgacg attgctactc aatcgaaagt
    23821 gagttagtgc gtatgaactt gcttctgaaa acttatgaaa tgaaagacct tttacatctt
    23881 gcagaaatcg ttactgggat taaactaagc aataaaaact aatgttcccc tcattctttg
    23941 aacttctact atttgattta tttattctga taaatgacga tttcaccttc aggatcttca
    24001 aatcccgtat agaatttatt taagatctct tcagtatcag taataggata ggcgataggc
    24061 cacataccct ttgatggtta gaataaactg ctcaaaatct gtatcgtatg ttggctttag
    24121 catattaaca aaggctatct ccaaattata aaccataagc gttgaatcgt tcttaacaat
    24181 ttcaattctt cctaacccaa agtcagaaga tataattacc ggacttgcct tacccttcat
    24241 gtgggttgtt gagattccat atttaaaggg aattgtcggt actatcggcg gtctgaaaaa
    24301 catcccaaaa ataaaaaaat ctttcccctg ctagtctcct aagtcccttt gtagcggata
    24361 tggcaccata atctcatttt atcgaagtga gtcatggttt atctgttggc caaccaaaat
    24421 tgatacaatc taacgatgca ccctctacgg cattctaggg gagtgcactt gcattcaccc
    24481 aataaaaacc ggcagttagc aaagaactta tgtagtcgta tgttcttact gttaacatac
    24541 aagaggtctc ctttccacaa ttgcttagca gtaaacggga atttacaaag ctaatctctt
    24601 ccattttctt atctttgttc taaaagtccc aaatggagca actgtattta catgaataaa
    24661 tttatatact tcccatacag ccgttttggt tgcctcgtct gcccattttc ttttgtgtgc
    24721 agtgaataga tcttcatcac tcattgaatc aatcatcatg ttaatatcat caatattacg
    24781 attgagaatt tcctctaatt ctttcaatgg tagatgtgca taggtatttg taaaccactt
    24841 atataactct cctaactgat tccatttaaa ttgatcagaa ggcgttttaa cagataatcc
    24901 atttctttca tcattttccc attttagaat taaattcgtc caaccaactt gatatgctaa
    24961 attttcagca ggtgttctat ccacttcctc aactctttta tccttcaatt cttcaggaat
    25021 agtttcaaat tctaaaatat atttagtata agtcctattt atttcttttt ttaattcttc
    25081 tttattttta tatgttctca ttatattacc tccataaatt cctaattgtc tcctttaatt
    25141 catctataat ttcatccacg atattttaat ccgttctaca atgcatatct gatatgcatt
    25201 gactgtctaa ctcatatgtg aatgatttat aaaatatccc aacgtctcat ctaaagaaat
    25261 gttgatcgtc agcaaggagc taaaaaatac gaatatattt cttttgtagt aaaatcggac
    25321 tggtttgcat atcattttct tcgtaaatat caaaatcatg ttaactacta tcaattacta
    25381 ataaaaacta ccactttaag cgttaagaac tgttaagttt agttaagtga ggatagttca
    25441 caaaatccat tgttaaactt cacgaaagtg gtaaaactca aaatgcttta tataggatta
    25501 cagcatcgct ctttcttcga ttgcgcgttg ggtaaaaaat attatgaagt aaaaattgac
    25561 gatgacacgt tccttacggc tcatcagatg aaacagctac aaaaacgaaa tgatcaactc
    25621 gaagaaaatt taattctaaa aaagcgattg ccatattcac gcctcgctcg acgaactatt
    25681 aaaggtcaag atttacctga agatcatcct ttttaggaaa ttgaagcttt ttatgttgca
    25741 caatgcattt ataatactac aatgatgttt tcaccagata ttcttctagg gtgcggtgta
    25801 atgaaacaga tacacttgca aaagaaaatt caccgcgaat ttactaaaat tatgaataat
    25861 tatgttaatc ttccgaaatt tttttccaaa attaagggat caagctggtg ttatagattg
    25921 tttaaggttg gcaaaaaaat aacttctttt taaacaatta tagaaaaccg atactctgtt
    25981 aagttggttg acacggtgtc gatttttatg actttcttat tctgaaactt cactaacgga
    26041 cagtacctaa aaagtctata gtatggacgt ttacgagtac tattttatca aaaatgctgc
    26101 tcatttcaac ttatatgttt tagtgaacta cccccactta gc
//
LOCUS       DAZBSA010000033        25998 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000033 DAZBSA010000000
VERSION     DAZBSA010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.033"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            289..606
                     /locus_tag="ACHGMT_001242"
     CDS             289..606
                     /locus_tag="ACHGMT_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFN6834996.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(697..1062)
                     /locus_tag="ACHGMT_001243"
     CDS             complement(697..1062)
                     /locus_tag="ACHGMT_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834997.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1270..1752
                     /locus_tag="ACHGMT_001244"
     CDS             1270..1752
                     /locus_tag="ACHGMT_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6834998.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     LVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1949..2839
                     /locus_tag="ACHGMT_001245"
     CDS             1949..2839
                     /locus_tag="ACHGMT_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFN6834999.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            3165..5639
                     /locus_tag="ACHGMT_001246"
     CDS             3165..5639
                     /locus_tag="ACHGMT_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFN6835000.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(5742..7478)
                     /locus_tag="ACHGMT_001247"
     CDS             complement(5742..7478)
                     /locus_tag="ACHGMT_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN6835001.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7475..8179)
                     /locus_tag="ACHGMT_001248"
     CDS             complement(7475..8179)
                     /locus_tag="ACHGMT_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN6835002.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            8310..8813
                     /locus_tag="ACHGMT_001249"
     CDS             8310..8813
                     /locus_tag="ACHGMT_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFN6835003.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8773..9111
                     /locus_tag="ACHGMT_001250"
     CDS             8773..9111
                     /locus_tag="ACHGMT_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFN6835004.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            9132..10550
                     /gene="ffh"
                     /locus_tag="ACHGMT_001251"
     CDS             9132..10550
                     /gene="ffh"
                     /locus_tag="ACHGMT_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFN6835005.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10662..11240
                     /locus_tag="ACHGMT_001252"
     CDS             10662..11240
                     /locus_tag="ACHGMT_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFN6835006.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            11244..11765
                     /locus_tag="ACHGMT_001253"
     CDS             11244..11765
                     /locus_tag="ACHGMT_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFN6835007.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11844..12398)
                     /locus_tag="ACHGMT_001254"
     CDS             complement(11844..12398)
                     /locus_tag="ACHGMT_001254"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFN6835008.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12651..12926
                     /gene="rpsP"
                     /locus_tag="ACHGMT_001255"
     CDS             12651..12926
                     /gene="rpsP"
                     /locus_tag="ACHGMT_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFN6835009.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12938..13189
                     /locus_tag="ACHGMT_001256"
     CDS             12938..13189
                     /locus_tag="ACHGMT_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFN6835010.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            13313..14104
                     /locus_tag="ACHGMT_001257"
     CDS             13313..14104
                     /locus_tag="ACHGMT_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835011.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTATTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            14274..14795
                     /gene="rimM"
                     /locus_tag="ACHGMT_001258"
     CDS             14274..14795
                     /gene="rimM"
                     /locus_tag="ACHGMT_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFN6835012.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14785..15546
                     /gene="trmD"
                     /locus_tag="ACHGMT_001259"
     CDS             14785..15546
                     /gene="trmD"
                     /locus_tag="ACHGMT_001259"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFN6835013.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15682..16029
                     /gene="rplS"
                     /locus_tag="ACHGMT_001260"
     CDS             15682..16029
                     /gene="rplS"
                     /locus_tag="ACHGMT_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFN6835014.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            16339..16581
                     /locus_tag="ACHGMT_001261"
     CDS             16339..16581
                     /locus_tag="ACHGMT_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFN6835015.1"
                     /translation="MNDGKVKQVPSSTKKKNILLKEVLKRFDHGVTYTETEVNSILLN
                     VFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(16669..16815)
                     /locus_tag="ACHGMT_001262"
                     /pseudo
     CDS             complement(16669..16815)
                     /locus_tag="ACHGMT_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            16872..17186
                     /locus_tag="ACHGMT_001263"
                     /pseudo
     CDS             16872..17186
                     /locus_tag="ACHGMT_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345994.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
     gene            17215..18474
                     /locus_tag="ACHGMT_001264"
     CDS             17215..18474
                     /locus_tag="ACHGMT_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN6835016.1"
                     /translation="MKRWKKMMIGSIVLGMSLVFGACGNQEDSSEESANEITVSTWNY
                     ETTPEFKALFEAFEKKTGIKVKAVDIASDDYDTKLTTMLSSGDTTDVLTMKNLLSYSN
                     YALRDQLVDQTERIRELDTDAAEETYEMYDIDGKTYALPYRTDFWVLYYNKKMFDDAG
                     IEYPKNLTWDEYEDLAKKLSKNDGQVYGAYQHIWRSTIQAIAAAQNEANLVEPDYQYM
                     TDYYDRALRMQKEGAQMDFGTAKSTKVTYQSQFEEQKAAMMYMGTWYMAGILANKEAS
                     KTEVEWGITAIPQKKKGESVTTFGSPTAFAVNKNSKKQKAAQEFIEFAASEEGAKVLA
                     GVGVVPSYRTDEIDQLYFNLAGMPTDEISKKAFSPDEIKLEFPIDTYGPAIDKILQEE
                     YDLVLVGDETPEKGTVNMEKRVAAEKE"
     gene            18527..19423
                     /locus_tag="ACHGMT_001265"
     CDS             18527..19423
                     /locus_tag="ACHGMT_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6835017.1"
                     /translation="MENSINKKATNHLRRKRTWIAYSFIAPNFIGFFVFTLIPILFSL
                     VLAFMKWDSFSTPEFAGLDNFRKMANDETFWISLKNTFLYTAAVVPGTLVCSLGLALL
                     LNQKIRGMKLFRTIFFFPYVTSLVAIAVVWNMLFHPTVGPINIFLRRFIEHPPGWTSS
                     SNWALWAIVIVSIWRGMGYYMILYLAGLQSIQKELYEASSLDGANRWKQFLNVTLPSL
                     RPTTFFVTIMLVINCFKVFDLVQVMTDGGPGRATNVLVYQIYNEAFKKFDFGYASAIS
                     LVLFVIVLGVTVVQFSWNKRRT"
     gene            19434..20303
                     /locus_tag="ACHGMT_001266"
     CDS             19434..20303
                     /locus_tag="ACHGMT_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6835018.1"
                     /translation="MEESVRNERKKAKIRSKNMPFIQWLGMILLIVLALLTIIPFLWM
                     VSASFKANNEVLTIPIQWIPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFT
                     SSFAVYGFSELNFKGRDILFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQ
                     AFNAFGVFLLKQYYSSIPDSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVN
                     TWNDYMGPFIYLSSTENKTIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQK
                     YFVEGIASSGMKE"
     gene            20313..20988
                     /locus_tag="ACHGMT_001267"
                     /pseudo
     CDS             20313..20988
                     /locus_tag="ACHGMT_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
     gene            21194..22858
                     /gene="alsS"
                     /locus_tag="ACHGMT_001268"
     CDS             21194..22858
                     /gene="alsS"
                     /locus_tag="ACHGMT_001268"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFN6835019.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            22871..23581
                     /gene="budA"
                     /locus_tag="ACHGMT_001269"
     CDS             22871..23581
                     /gene="budA"
                     /locus_tag="ACHGMT_001269"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFN6835020.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            23711..24208
                     /locus_tag="ACHGMT_001270"
     CDS             23711..24208
                     /locus_tag="ACHGMT_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFN6835021.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            24393..24653
                     /locus_tag="ACHGMT_001271"
     CDS             24393..24653
                     /locus_tag="ACHGMT_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835022.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(25015..25254)
                     /locus_tag="ACHGMT_001272"
     CDS             complement(25015..25254)
                     /locus_tag="ACHGMT_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835023.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            25540..25854
                     /locus_tag="ACHGMT_001273"
     CDS             25540..25854
                     /locus_tag="ACHGMT_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835024.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT         8635 a         4373 c         5306 g         7684 t
ORIGIN      
        1 agattacaaa tagtatcgta tttcgcaatc ttaaaattat cttcttactg tgaataagat
       61 aataaaattt gtatttgtcc tttttaggta acttatttta atctatatac ctttctggat
      121 caacgaaagt atactttata tagtcataac gccgatggta actatgcact gtaacgataa
      181 tgctgctaaa atatcttttt acgaaaaaca tcacaaggaa aaacttaact atggtattat
      241 ctaaacataa ttttttcggt atttaatatt taattaggag gtaatatttt gcttttttat
      301 agtaatttta tcttaatagt agcgatatta ttacttctga atatatggat ttttgatagg
      361 agtagaaatg caagtatagg ttttagaact aaacgaagtc tgtcttctaa aaaaaattgg
      421 gtatactcac aaactatttt ttatggagga attgttttaa ttagtttact ttcttcaaca
      481 ctttattctt tgaatattat agatgtttcc acctcaaatt caatatctat tattggaatc
      541 attattgctg caattattac acaactgttt ttagtgtttg gagagaagaa aagaagtaaa
      601 aaatagaaaa actagtgata gttttggtga tataagaaaa acatatatgt ataaaaagaa
      661 gaaagtagct tctctttgta catatatggt ttgtttttat ggatgccgag cttttttata
      721 agataaggct accgcccata acgacccaag gatttgtgga actaagtaaa tacctgcaag
      781 aagcaagatc agctgggtcg gttttaaagg atgatagaag taacgccatt cagtaaaatt
      841 ggcgcaaaat aaataggaag tgacagtaga tataatattg gcaatgaata aagatataat
      901 acgcctgttc ctttgaaaat aatatccagc aaaaagagat aaaaatatca atgtgatgaa
      961 cccgcttacg gaatgataac gaaaatcaag cagtaaggcg aaatgcacat aaggaacaaa
     1021 agataaaagc aacggaaggt ttttccagca agtttttatc atctttcatc accacctaac
     1081 aagtgttatt attttgttag ttttattgta aattgaatcc tgcaataatc aattactttt
     1141 tcattatttc tagattaaat gaataaactc tcatgccatt atctctaaaa aaaaagtttt
     1201 tccccctaat ttttttagat agaaaattgt atagtatgat gagagaaaaa gtagtttggg
     1261 ggaacgttaa tgagaagaaa agcagtaatc gatcggcttt atttattttt ttggtttttt
     1321 cctatcgttt tgctaccttt tacatgggta aatgctgttg tacaaggtgc ggagatatta
     1381 ggcctgatcc aaggaagcgg attagtggtt tctaggaatt ttccagcttt gactgttttc
     1441 tatttagtga taagtgtagg tctcttatta ccaaaatctc tgatcaatca ctacaattgg
     1501 cagctgcttt acttttcttg cttattagta tttacatcta tgttttcttt gctgcctcgc
     1561 ttgattatcg gccctaatgg tttgagcgag agtggattga aggattcagc ttattcctcc
     1621 atgtatttct tgacgatcaa accatggttc tatttagtaa ttgcctcatt tattcttgca
     1681 acattttttt acttctggac agaaatcagg ataaaaagtg aacgatctgt atctataaaa
     1741 aataactatt gatggtggat catcagtaaa aaagcatatg agctgcgtaa aatgcatgtt
     1801 tctcatatgc tttttttatt attatgtaaa gaatgcgaaa aacgtttaca tgatttttac
     1861 ataacttaac tatattttta cagcaaccat acgagggatt tacagtcgac tgataaactt
     1921 tagttgtaaa taagaaagga gcaataaaat gaaaaaagta ttgtttactc taggtatgat
     1981 gggaattctt ttagccggat gcggctcagg atcaggaaat acagatggct ctgccacaca
     2041 aaataattca agtgactcaa atgaacaagt aaaaatcgtt gcggtaggtt cgacagcatt
     2101 acagccgtta gtcgatgcag cacaagaaag ctttgtacaa gaaaatccaa attatcaaat
     2161 ctctgtacaa ggaggaggaa gcggaactgg tttgagccaa gtcgaagctg gagcagttac
     2221 tatcggaaac tctgatgtat ttgcagaaga aagagacgga gttgatgctt caaaattagt
     2281 ggatcataaa gtagcagtgg tcgggatggc accaatcgtc aataaagata cagatgtcaa
     2341 agatatcacc aaacaggaat tgattgatat ctttacagga aaaatcacta actggaagga
     2401 agttggcggg aaagatcaaa aaatcaacgt cgtgaaccgt gcaaacggca gcggtacccg
     2461 cgcgacattc gaaaaatggg gactggatgg tgctacccct gtgcagtccc aagaacaaga
     2521 ttcatcagga acagttcggc agctggtaag tcaaacacca ggagcaatca gctatttagc
     2581 tttctcatat ctcgatgatt ccacacaagc actaagtatc gacggtgtag aaccaaaaga
     2641 agagaatgta gcagataaca gctggggaat ctggtcatat gaacatatgt acacgaacgg
     2701 aaaaccttcc cctgaagtcc aaaaattctt agactacatg atgacagaag aaatccaaga
     2761 aggacctgtc aaagaattgg gctatttacc aatcacaatg atggaagtag aacgtgatca
     2821 cgaaggaaat atcaaataac tagcagataa acatgccacg gtagtcgtta ctattaagga
     2881 aaaatcaact atgtttcccc acatatacat ttttccttta attgtgaaaa atagttcctc
     2941 atattttgcc gttttttgag ctaataccga gggtcagctg attgaacacc gtttattcga
     3001 aaaaaagagc tacgacatag aactgtcgta gctttttttt tgctatatga caggaatgaa
     3061 aggtgtttgc aagcttaatg tgaggaacac tttccatatc tgttctatac tggataggag
     3121 aagtttggaa atatccagaa aacgaaatca atcgaggtag ccacatgaag aataaatgga
     3181 taaatcgaag taaaaaaaag aaagtaacac aaagatcatc agataaagtc atccgtcttt
     3241 ttccatcgta taaagaagga ttaacggcag atcaagtcaa ggaacgaata gaaaaaggtg
     3301 cagcaaataa ttcagtcgat cccacattca aaacaaatca gcaaatcgta ttggaaaaca
     3361 tatttacgta tttcaatttg atctttctta tcttagccat tttgctttgt ttagtagaat
     3421 cctataagaa cttgactttt ttaccagtta tcatagccaa tacaggaatc gccatttttc
     3481 aggaaattcg atccaaaaag atcttggacg aactaaatgt tttaaataca gcaaaagtca
     3541 atgtcatacg agacgggata gaacaaacaa tagatataga agaccttgta atagatgaca
     3601 ttgttatttt agagactgga catcaaattc cagcagatgc agaggtcgtg gaagggaaac
     3661 tgcaagttaa tgaggcttta ttgacaggcg aagcagatga aataaccaaa gaaataggcg
     3721 atccattaat gtccggcagt ttcattgttt ccggaaaagc ttacgcccga ttagacaaag
     3781 ttggagcaga ttcgtatatt tcccagctga cagtcaaagc caagacaatg ggagaaggag
     3841 aacagtcgga gatgattgct tctcttaatc aattgatcaa atggattggg atcatcatta
     3901 ttcctatcgg tatcctgctt ttttctcaaa gttacttttt taatggaaac acgatcaaag
     3961 aaagtgtcgt tgcaatggaa gcagcattga tcggtatgat acctgaagga ctgtacttgc
     4021 tgacgacgat tgcattagca ttaagtgcga ctaaactagc aaagcaaaga gttcttttac
     4081 acaatatgaa aagcattgag acattggcaa gggtaaatgt cctttgtgta gataaaacag
     4141 gaacgatcac agaaaataaa atgtctgttc aaaaagtgat cgtctctaaa aaacaagaaa
     4201 taatcagtca gtcagatcaa ttggaagcat gcattgctga ctatgcaaaa gcgatggcag
     4261 gagacaatgc aacaatggaa gctgttaaag catattttac aaaaaccact gatcaatcct
     4321 atcaccatgt tattcctttt tcttctgtcc aaaaattcag cagtgtagcg ttggcagaca
     4381 aggtgtatgt tttaggagcg cccgaaatgg ttttgcgcga tcaattgccg gaatatgcgg
     4441 aagagtttgt ttctttcgct gaaaaaggct atcgcgtgtt ggtctttggt gtgtacgatg
     4501 ggattcttga agaaccagta ttgacagaag cagtcacacc attaggctat attctgattg
     4561 ctaatcctat cagaaaagaa gcaagagcca catttgatta cttcaaaaaa cagcaagtag
     4621 ccatcaaagt gatttctgga gataatccgc tcaccgtatc caatgtagct gtccaagcag
     4681 ggatttcaca tgctgatcag tacgttgatg catctaaatt atcagaagaa gaatatgaag
     4741 cagctgtcga aaaatataca gtttttggac gcgtcaagcc agaacaaaag aaaatcttcg
     4801 ttcaattgct aaagcgtcgt aacaatacag tagctatgac aggtgatggc gtgaatgata
     4861 ttctagccat gaaagaagca gactgcagta tagcgatggc ttccggaaat gaagccgcca
     4921 tgcaggcctc acaagtggtc ttacttgatt ctgacttttc acgcatgcca gaagttgttg
     4981 cagaaggacg gcgcgttgtg aacaatattg agcgatcagc cagcttgttt ttagtgaaga
     5041 atattttctc ctttttgctc tcactgtttt cagtgatctt tgctctgacc tatcctttag
     5101 aaccatcaca aatcacattg attagtttgt ttactatcgg acttccatct ttcttgcttg
     5161 ctctagaaga aaacaaaaag cgaatcagag ggaaattcat catgaatgtg atggaaaaag
     5221 ctgtgccagg tggactaaca gatatgatcg tagtcggtgc gttggtgatc tgcggggtga
     5281 ctttgaatct aaataaaaca gatgtttcta cagcatctac catgcttttg attgcagtag
     5341 ggttcctcgt tctctataaa atctgttcac cgctgaataa attcagaagt cagatcattc
     5401 ttttctgtgc tagcggtaac tttttttcgg tcatcttttt gcataagctg ttttcaatca
     5461 ccggtatctc agctgtatca cttttgctgt tgagcattct atttttctcg gcagattcga
     5521 tttttaggca tttgaccaca ttggtagaat atttgtttga acgtaaaaat gaaaaagagc
     5581 aaaagccgtt tagtcgcgta gttaaaaaat ttctatcgat atttaaacga gaaaattgac
     5641 gaaaagtatc tcaaaaaaga gtaaaaaaga acaaccactc gtgtatcttt cgaagcagga
     5701 tgagtcatcc aactttaaaa cgcacgagca gttgctcttt tttacatttt cggaatggta
     5761 acggtgaaag tcgaacctgt tccaagatga ctgtctacag taatcgtacc atgaaggagt
     5821 tgcacgtagt cttttacaat cgctaaccct aatccggtcc cgcctgaatg ccggcttcgc
     5881 gccttgtcta cgcggtaaaa acgctcaaag atccgttcct gatcctcaat gtcgatacct
     5941 ataccaaagt cttggacaga aaacgataga tcatctgttg cctgatagcg gataataatc
     6001 tcgctgtcgc tttttgaata ttggacagca ttttcaatca gatttttgat aattggataa
     6061 aggatatctg ttttagtagt aaatggttcg ttctttccaa tcaatcgaat cgtcaaccgt
     6121 ttcttttcga tgattggctg ataattttga acgatttgct ggaaataagg ttctgtataa
     6181 attgtctgga tctcgtaaga attctcgcca tcttttgaca gctggatgat ttcccgaatc
     6241 agtttatcta accggatcgc atctttttgc atgatctcaa gaaaagaagt cagagtttgc
     6301 gggtcatctt ttgcaccatc cagtaaagtc tcggtaaaac caatcagtga agtcacagga
     6361 gtttttaatt cgtgagagac gttgccaacg aaatcctttt gtagcttttc taaacgtcgc
     6421 actttcgtta agtcatacgc cacccctaga atctgtccgg taccttctga attagtaaag
     6481 tagcgcaaac tcaagtctaa tgtctgccga ccttctgtaa gggtgatctc ctgatggatc
     6541 agcggatgct caggagtcac ttgatgaatc agctgaatga gttttggctc ttgaatgact
     6601 tctgtgtacc gctgttcagg ttgatagctt accacgttta aatgtagctg catctttgga
     6661 ttaagcagta gtaaacgaga atcagcatca tcaatcagga atacgccaat catcagttcg
     6721 ttcagtaacg tatataattg ctcttctgtg gtagtatacg cccgatacat cttactcatt
     6781 tgttcgctca acgcattgat cgtctgatac aattcttccc attgatcggg tgtttgcatg
     6841 attacttccg cctgattagg attttgcacc atttttttta gtacgggcag cacggttttc
     6901 aatggctggt ttttttgata gatcagataa tagatcattc ctgcaagaac aaggaagaaa
     6961 gacagaaaga agaaaaagac ccaacggcga aaactctcag ttctcggtaa aaaaccgctt
     7021 gtcggttctg cgatacgtaa aatggcttct aactggccac tttttttgac tggtaatgcg
     7081 acatataaaa gatccccatt caaagtagta cttttgcgca aagcgctacc taggttccca
     7141 ccattcaata cggctttgat ttcgggacgg gagtttcgct gttcattcaa tgtttcattg
     7201 tttgtgtcaa ataagatctt tccagtagca tccattaaag taatccgttc gttcgactga
     7261 tggacgaatt cttccaaagc agttttgttt tgcgctgctt ggaggttttc gacatctaat
     7321 tgccggatca gcagttgtgc tttttttgtc aggtagcttt cttgctgttc cacgatctgc
     7381 tgttgaaaat aatgggaaat caattgccag ccaccaaaaa acaaactaac taaaagaata
     7441 agaaagaaga gacgttcata aatttttttc catttcatcg tttgggttcc tgaaacttat
     7501 agccaaatcc tctaatagtc accaagtact ttgggtgttt tggatctcgc tcgatttttt
     7561 ctcgcaaatg actaacgtgt acatctacga ttcggctttg tcctgcgaag tcaaaattcc
     7621 aaatgcggtc tagcaatgtg tctcggtcga tcacacggtc ttttcgtttc atgaaataaa
     7681 caagtaattc aaattctttc ggtgttagtt caatcagatg gtcgtctacg gtcacttgat
     7741 aattggtcaa atcagcagtg atttgtccaa tcgatagata ttcaggctca gcttcatctt
     7801 ttgattgctt acgaggttca attcgtctga atattgcttt catacgggct aatacttcgc
     7861 gaggactaaa tggtttggtc aaatagtcgt cagcacctat ttctaggcca atgatccgat
     7921 ccacttggtc atctttagcc gtcagcatca aaatcggtgt ttcatttttt tcttgacgca
     7981 gccgctgagt gatctccatg ccatccatcg ttggaagcat cacatctaaa atgatgaaat
     8041 caaatgattg ttctaatgct aattctaatc cttcgcctcc atctgtggca ctggttacct
     8101 tgtagccttc tttttccaaa ttgaacgtca agagcgttac aatagaaggt tcatcatcaa
     8161 caactagtat ttttttcatt caaatctcct ttaaataggt ttcattctgt ttatatttta
     8221 gcatagaatc agtacaaagg gctattttac aaaagaaata aatggtataa taggaaggca
     8281 ctttgctgaa tagaaaggaa gagcagtcaa tgattggaat cagtgcatgt ttaggcggtc
     8341 tggcctgtcg ctatgatgga cagaaaaaag aagtaccaga attgatggaa ctagttgaaa
     8401 ctggagaagc tgtcatggtc tgtccagaag tgatcggcgg attaccaatt cctagagatc
     8461 ccgctgaaat cattggtgga aatggattcg atgtgtggaa agatcaagca aaagtatgga
     8521 cgacaagcgg cgaagatgta acggaagcgt acaaaaatgg cgcgatcaaa gcctatcaaa
     8581 aattgcagga aaaacacatt gatctagtca tcatgaagga aaaaagccct tcatgcggaa
     8641 cccattcgat ttatgacggg agtttttccg gaataaaaaa aaatggtgcc ggagtggcta
     8701 ctgcttattt tattcagcaa aaaatgactg tgctctctga tgaagaatgg ctgaaaacta
     8761 gaggtgagca gaatggaaat cgaaaagacc aatcgaatga acgcgctctt tgaattttat
     8821 tcgaccttat tgacagaaaa acaaatgaac tacatggaat tatattatgc agatgatttt
     8881 tcattaggtg agatcgcgga agaatacgaa gtaagccgac aagcagtata tgacaatatc
     8941 aagcggacaa gcaagatctt ggaggattat gaaaaaaaac ttcatctttt ttctaattat
     9001 attgttcgag aacaggtatt ggaaaatatg actgcatata ttacagataa gtacccggaa
     9061 gataaaaaac tacttgaata tgtacagcaa attcaagcaa tcgaagaata aataaaagga
     9121 atgaagaaaa tatggcattt gaaagcttaa ctgaacgtct ccagcaggcg atgggaaaaa
     9181 taagaaaaaa aggaaaagta tcagaagctg acgtaaaaga aatgatgcgg gaaatccgcc
     9241 ttgccttact agaagccgat gttaacttgc aagtagtcaa agattttact aagcgcgtac
     9301 gcgaacgtgc ggtaggggca gaagtgctgg aaagtttatc acctgcacaa caaatcgtaa
     9361 aaatcgttga tgaagaattg acgatcacac ttggttctga aacagcagag ttaaataaat
     9421 caccaaaaat accaactgtt atcatgatgg ctggtcttca aggggcaggt aaaacgacct
     9481 ttactggtaa attagccaat tatttaaaga aaaatgaaaa tgctcgtccg ctcctaatcg
     9541 caggggacgt atatcgacca gctgcgatcg accaattgaa agtattaggt caacagctag
     9601 acgttcctgt atttgatatg ggaactgaag tcagcccggt agagatcgtc cgtcaaggga
     9661 tggagttagc aaaagaaaag aaaaacgact atgtattgat cgatacggca ggacgtctgc
     9721 atatcgatga aacattgatg gatgagttga aacaaatcaa agaattgacg caaccaaatg
     9781 agattttgct tgttgtcgat gccatgactg gtcaagatgc cgtcaacgta gctgatagct
     9841 tcaaccagca attaggtatt acaggggtcg tcatcactaa acttgatggg gatactcgag
     9901 gcggggcagc actatcgatc cgctcagtga caggcgcacc aatcaaattc atcggttctg
     9961 gtgaaaaatt gactgactta gaagtattcc acccagatcg tatggctagc cgtatccttg
    10021 gtatggggga tatgctgaca ttgattgaaa aagcgcagca agactatgac gagaaaaaag
    10081 cggaagaact tgcacagaaa atgcgggaaa attcattcga tttcaatgac tttattgaac
    10141 agcttgatca agtgatgggt atgggcccac tggaagatct aatcaaaatg atcccaggaa
    10201 tgaatcaagt ccctggaatc gaaaacgtca aggtagatcc aaaagatgtt gaacgcaaaa
    10261 aagcgatggt ttattctatg acgccagcag aaagagcaaa tcctgatctg ttgaacccaa
    10321 gccgtcgtcg ccgaattgct gccggttctg gaaacagtgt tgttgaagtg aatcgaatga
    10381 tcaaacaatt caaggaatca agaaaaatga tgcagcaaat gtccaaaggg gatatgaaca
    10441 tcccaggtat ggaccaaatg tttggttcag gcgtcaaagg aaaactaggc aaaatggcga
    10501 tgaatcgaat gatcaagaaa aataagaaga aaaagaaaaa acggaaataa gatgaaaaaa
    10561 acagcttcgg tacagactga aaatgtctac tgccggagct tttctatcag caatttttat
    10621 tctttactaa tacaattact ctaaacgaag aggtggcaca gatgagatgt ccgtggagtg
    10681 aacaaacaga aagtatgcgg caatatcatg atactgtctg gggtgttcct gaatatgatg
    10741 accaaaagct gttccgaaaa ttgatgcttg atattaatca agccggattg agctggcaga
    10801 cgattttaaa taaaagtgaa gcattcgatg aagcatatga tcgattcgat attaaaaaag
    10861 tcgcaaatta tggagaaaca aaaatcagtg aactaatgga aaacaaagga attattcgta
    10921 accgaagaaa aatcgaagca gccgtccaaa acgccaaagc agtcttgaaa attcaagaag
    10981 aaatcggcag tttttcagag tatctttggt cgttttctaa tggaaaaatc gttgattctt
    11041 cttatcattc acaagaagaa gtcccaacaa caagtcctct ttcagatgaa ttagcaaaag
    11101 atctaaagaa aagaggattt aaatttatcg gaagcacaac aatctatgct tttttagaag
    11161 ctgtcgggat catcaatgat caccttgaca gttgtttccg caaagaagag gtcgtacgta
    11221 tcggcagaaa gggcgaataa agcatgcgaa aaatcatatt ttatggtgct ataagtttag
    11281 atggctattt ggcaacgaaa acggatgatc ttcaatggct ttttgacact ccaacaggta
    11341 agcaaaccac ttatgaagct ttttatgaaa caatcgatac gaccatcatg ggcagaaaaa
    11401 cttatcagga agcaaaaaaa tatttggaga ccgaagcaat ttatccagag aaaaagaatt
    11461 tcgttttttc cacccacgca caattacaat tgccagatgc tgaggtcgta aacgaagatc
    11521 ctgttgcttt tctaaaaaat ctacaaatga cagaaggaaa agatatctgg gttgtgggtg
    11581 gcggtcgatt gatcaaaccg ctattggaac atcaaatgat tgatgaatgg tatatccaga
    11641 ttacccctgt attattaggg gacgggattc ctttattcca aaaaggaaca tatgaagcgc
    11701 gttttgaaat gctagacacg acacgattcg gtgaatttat cgaattgcat tatgtacgaa
    11761 agtagaagta aataaaataa aaaaggcaac tatatgcaga aatgtcctgt acatagttgc
    11821 cttttttatt accgttatgc tttttactct gttaatttgt tagaaaaatc tagatacgca
    11881 tctaaaatcg tttggaagaa acttaatgtt ccttcaacga tattaccatt ttcatcaatt
    11941 agattcgcaa cgtttccgat atatgcttcc ggttgctgca aggttggcac attcaaaaag
    12001 acaagtgatt gtcgtagatg gtggtttgcg ccaaagccgc cgatcccacc tggtgatgca
    12061 gtaacgacta aacctggttt atgatcccaa acactgtgtc cataaggtct tgagcctaca
    12121 tctaaagcat ttttcaatac tgctggcaca gaacggttgt attcaggagt aacgaaaacg
    12181 acaccgtcta gtcccctcac ttcctcacga aaacgagtcc attcagcagg agcctgttct
    12241 ggagtttcga gatcctcatt gtaaaatggc agatcatcga tttttacaaa tacactttcg
    12301 tacccttcag gaagcaattt cgcaaaagct tctgctactt ttttgttgta agaatctttt
    12361 cttaaactac caataaaaaa tccaattttt tttgtcatac gatttttcct ccttcaaact
    12421 atctataaca atcttacaaa aaataagtaa ctggagcaaa taaaaacact tactgacaaa
    12481 agctacatag aagatagatt acttattttt aacaaaaaag aagtctaaaa tcttctattt
    12541 cctaaaaaaa atcgaattta atcaagctgt aaagaaaaaa agctttacag ggtaaaaaaa
    12601 aactgttaca ctacatcttg tgaataaaac aatggaggtg gaaataaata atggcagtta
    12661 aaatccgttt aaaacgtatg ggttctaaaa agagtccgtt ttaccgtatc gtcgtagctg
    12721 attcacgttc tcctcgtgat ggacgtttca tcgaaactgt aggtacttac aatcctttga
    12781 aagaccctgc agaagtagtt ttaaaagaag acttagttct tgactggtta tcaaaaggtg
    12841 cacaaccttc tgacactgta cgtaacatcc tttcaaaaga aggcgttatg caaaaacacc
    12901 acgaagctaa attctcaaag aaataaggtg accggatatg aaagatttaa gcgacttagt
    12961 cttaacaatc gttcgtccgc tagtcactta tcctgatcaa gtcaagttag acgtcgtaga
    13021 gtcgaaagac ttttacgagt acaatctaac agtggctcct gaagatattg gccgtatcat
    13081 tggaaaacaa gggcgtgttg ctaaagcaat ccgcacaatc gtttacggtg ttcggatcaa
    13141 tgcaccgaaa aaagtgcgtt tgaatatcat cgatgataag gaaaagtaaa taaacgtgct
    13201 gtactcgaat tttcgagtgc agcttttttg tttttttatt aattgaagaa atgattattt
    13261 ggcaaaacaa gcaagaaagc gtatccttat aggaggaggg agtgaacagc caatgagaaa
    13321 gaaaatgtta tttattccat ttgctctatt gttaagcgct tgtagttcgg ttactcaaga
    13381 atcagacaat acacatctat caagcagtat atctgaaact gttaccagtc cagaaaaaag
    13441 tagtaccaaa acggctacca ccactgaaca aacaatggct acctcaaata atgagaaaaa
    13501 gactgcttta gaccagttga aagaacaaca gccaaatgtt cccatgcctc tagatgtacc
    13561 agtctcttca ggctatctca atatcgcagc gacacataca aagcaaggtt attctatttt
    13621 atattacaga acagatcgtc ccctcgggtt gaatgctgac gaattgaatc aagaaacgcc
    13681 tattgcaacc tatctatatc aatatgggtt tgcttctagt caagaaacaa tccaagtttt
    13741 gcagcccttt gagatcgaca caaatggtca gcaagtggac ttaggttctc gaatcacagg
    13801 gtatcagcaa ggtgcagcag gttcaagttt tcttgaatgg caagaaggaa actggcgtat
    13861 tcgaatccgt gggaataata tagaaggcca agatccctta ctattagcta aagaaatcgt
    13921 agcttatttg gaagaaaaca gtctaccagc acctgaacaa tttggtaaaa ttactgttga
    13981 tatgggtgat acgacgaatc gagccgtaga agtaagctgg caagaaccga aaaatgccta
    14041 tacgatcact catcaagatc ctatgtctgc gttgaagatg gcagtttcta tgaagaggtt
    14101 gtgaaatcag cgcgctgatc tgagcccatc atttaactcc aagtcacagg aagtaatcaa
    14161 aaatagcacg ccgcattttt gcataatttt ggcttaatac cttaaaagaa aactagaatt
    14221 gcctcttcac acatgctaaa ataaaacaga caaaaaagaa atggggatat aagttgactg
    14281 aatacttgaa tgtaggaaaa atcgtaaaca cacaaggtat caaaggcgaa gtccgtgtta
    14341 tctcaacaac ggatttccca gaagaacgtt acaaaaaagg aacggtgtta acactattcc
    14401 aagacggaaa agcacctgtg gaattaacag tcaaaagtca tcgcaaacat aaaaattttg
    14461 acttattgag ttttgaagga catccttcta tcaatgatgt ggaaaaatat cgggatggca
    14521 tattaaaagt gtcaaaagac aactctgtag atctagctga aaatgaattc tattaccatg
    14581 agatcatcgg tctgcatgta ttagaagatg gaactgagtt aggtaaagta aaagaaatct
    14641 tgtcaccagg tgccaatgat gtttgggtcg ttcaacgtcc gaaaaaagct gatatattgc
    14701 ttccatatat cgcttctgtc gtcaaagaag tcgatttaga agctggagtg gtccgtgtag
    14761 aaattccaga aggactgatt gacgatgaag attgatgtat tgactttatt tccaagaatg
    14821 tttgaaggtc ctatggggga atccatcatc ggtaaggctg tagataaagg attattagat
    14881 atcaatatct ctaacttccg cgattattca gataacaaac atcagacagt agatgattat
    14941 ccatacggcg gtggagcagg tatgctgctc aaagtccagc ccatctatga taacatccaa
    15001 gccattgaaa aagctgcacc ggatgtcaaa aaaagagtaa ttcttcttga cccagctggt
    15061 aaacgattcg atcagaaaat ggcagaagaa ttttcacaag aagaacactt agtattcatt
    15121 tgtggtcatt atgagggata tgatgaacgt atccgcacat tagtgacaga tgaagtttcc
    15181 ttaggagatt atgtccttac tgggggagaa ttaggtgcga tggtcatgat cgatgcaaca
    15241 gttcggcttt tgccagatgt gttagggaat cagacttcag ctcaaacgga ttcttattca
    15301 accggtttat tggaacatcc ccaatatact cgcccggcag aatataaagg gatgaaagta
    15361 ccagaagtat tgacaaatgg aaatcataaa ttgattgaag aatggcaatt aaaagaatca
    15421 ctaagaagga cctatcaacg tcggcctgat cttttggaaa cacttgaatg gacacctcaa
    15481 atgacgaaat ttttagaaga aatcaagaaa gaagaacaag aaaaaacagc agatgaagcc
    15541 aagtgacttt ttctaaaata ttctttacag taggttgata atatgttagt attttatggt
    15601 gagtgcaaaa gcacttaact attacgatat tccgctatga gaaaagctca taagaatatt
    15661 tggaataagg agaagaaaac catgaatcca ttaatcgaag aattaacaaa agaacaatta
    15721 cgttctgaca tcccagcgtt ccgccctggt gacactgtgc gtgttcacgc aaaagttgtt
    15781 gaaggtactc gtgaacgtat ccagttattt gaaggtgttg ttatcaaacg ccgcggtgct
    15841 ggaatcagcg aaacttatac agtacgtaaa gtttctaacg gagtgggcgt tgaacgtaca
    15901 ttcccattgc atacaccacg tgttgcaaaa atcgaagtag ttcgctacgg taaagtacgt
    15961 cgtgcaaaat tgtactacct acgtgcatta cacggaaaag ctgctcgtat caaagaaatc
    16021 cgtcgttaat cattgttatc agactgagaa agaacccttg gtattcaagg gttctttttt
    16081 cttagcatgt aatttgaaca ccctgatcca gtttttttct ttaattattg gtaggggaga
    16141 ataatggatt agaataaatc tacctataag tagtagtaaa aaaaagagca ctatgataat
    16201 atctttctta agagtaaccg ctttaataaa aaatccagaa tattctaaca taaatggagt
    16261 attcttagaa tcaaaaggag aaagaagatg gtttatactt atacggagca agaaaaaatg
    16321 aaagtttgag acacattcat gaatgatgga aaagtaaaac aagtaccaag ttcaacaaaa
    16381 aagaaaaaca tcctattgaa agaagtcttg aaacgctttg accatggagt gacttataca
    16441 gaaacagagg ttaattcgat actgctgaac gtattttcta gtggcgatta tgtcgaacaa
    16501 agacgttatc taatcacatt tggctttttc aaaagaagtt cagacggtcg ggcatatcag
    16561 atgatgggaa ttgaaaatta gaagttttaa tttatactca ttgaaaaaag ttcaagtatg
    16621 cgatattggt tctacaccgc atacttgaac tttttttgtc atctgtatct ataatacaaa
    16681 gccaagaacg ttattcaaaa tatggatagc tatgataaaa gctaatgagc gattcgcagt
    16741 taaataagca atcgtcagta aaatcgttcc aggagcgtac atgaaaaaat caattggatg
    16801 cgttacatga ataaagataa ttctctttta tttgtacgaa taaataccaa aacgtacctc
    16861 ctaaaattag attcaaagaa gatagcaaag aggaaaaaga tagttctgca gcagctatag
    16921 cggcatgtgg attgctagaa ctttccaaac aactgcctgt ttcagatcct cgtcatttcg
    16981 attacgaaga gatagcaatc aaaatcttgg aagaattaca gaaaaactat acaacgaaac
    17041 gagttaacgg atcaaacggt ctgcttctcc atgccgttta tgataaaaac agcttaaaag
    17101 gtgtagatga atgtgtgatc tggggcgatt acttttatgt agaaggaatc actcgtctag
    17161 ctaaaacatg gtactgttac tggtgatgta taagagaaga ggaggattca tataatgaag
    17221 cgatggaaaa aaatgatgat tggatcaata gttttaggaa tgtcactcgt atttggtgct
    17281 tgtggtaatc aagaagacag tagtgaagaa tcagcaaatg aaattaccgt ttcgacatgg
    17341 aattatgaaa caacacctga attcaaagcg ctctttgaag cattcgaaaa gaaaacggga
    17401 atcaaagtga aagcagtaga tattgcttca gatgattatg atacgaaact caccacgatg
    17461 ctttcttctg gagacacgac agatgtattg acgatgaaaa atcttttgtc gtattcaaat
    17521 tatgctttac gagatcagtt agtcgatcaa acagaacgaa ttagagaatt agacactgat
    17581 gctgctgaag aaacatatga aatgtatgat atcgatggaa aaacatatgc attgccatat
    17641 cgaacggact tttgggtact atattacaat aagaaaatgt ttgatgatgc aggaatcgaa
    17701 tatccaaaaa atctaacttg ggacgaatat gaagatttag caaaaaaact tagtaaaaat
    17761 gacggacaag tttacggagc atatcaacat atctggcgtt cgacaatcca agctattgca
    17821 gctgctcaaa acgaggcgaa tcttgttgaa ccagattatc aatatatgac tgattattat
    17881 gatcgagcat tgaggatgca aaaagaaggc gcgcagatgg attttggcac ggctaaatca
    17941 acgaaagtaa cctatcagtc tcaatttgaa gaacaaaaag cagcaatgat gtacatggga
    18001 acttggtata tggctggcat actcgcaaac aaagaagcaa gtaagacaga agtagaatgg
    18061 ggaattacag ctattcctca gaagaaaaaa ggagaatcgg tcacgacgtt tggctcacca
    18121 acagcatttg ctgtcaataa aaactcaaag aaacaaaaag cggcgcaaga atttattgaa
    18181 tttgcagcca gtgaagaagg agcaaaagta ttagcaggag taggagtagt tccttcatat
    18241 cgtacggatg agattgatca gctttatttt aatctcgctg gaatgccaac ggatgaaatt
    18301 tctaaaaaag ctttttctcc tgatgaaatc aaacttgaat ttccgattga tacgtatggg
    18361 cctgcaatcg ataagatttt acaagaagaa tatgacttag ttttggttgg agatgaaacg
    18421 ccggaaaaag gaacggtaaa tatggagaaa cgtgtagctg cggaaaaaga ataatcatga
    18481 aaggagagaa gccaagagat aaaacactct tggtttcgtc atcgaaatgg agaatagtat
    18541 aaataagaaa gctacaaatc atttgcgtag aaaacgaacg tggatcgctt attcgtttat
    18601 cgccccgaat ttcattggtt ttttcgtttt tacattaata ccaatcctgt tttcgcttgt
    18661 actagcattt atgaaatggg attctttttc aacgccagaa tttgctggac tagataactt
    18721 tcgtaaaatg gccaacgatg agacattttg gatttctttg aaaaatactt ttttatatac
    18781 cgctgctgta gtgcctggca cacttgtttg ttcgttaggg ctagctttac tactaaatca
    18841 gaaaattaga gggatgaagc tttttcgtac gatatttttc tttccgtatg ttacttcctt
    18901 agtagcaatt gctgttgttt ggaatatgtt gtttcatcca acagtgggac ctattaatat
    18961 ctttttaaga cgatttattg aacatcctcc aggttggact tcaagttcaa attgggcgct
    19021 ttgggcaatt gtaattgtca gtatttggcg agggatgggt tattacatga ttctatacct
    19081 tgcaggacta caaagtatcc aaaaagaact atatgaagcg tcttcgctag atggagcaaa
    19141 ccgttggaaa caatttttga atgtgacatt accttcgttg agaccaacaa ccttctttgt
    19201 aacgatcatg cttgtcatca attgtttcaa ggtgtttgac cttgtgcaag tgatgacgga
    19261 tggcggccct ggtcgagcga caaatgtgct ggtttaccaa atctacaatg aagcattcaa
    19321 aaaatttgat tttggttatg cttcagctat ttcacttgtt ttgttcgtaa ttgttttggg
    19381 cgttacggtt gttcaatttt cttggaataa gcgtcgtact taggaggtgt gatatggaag
    19441 agagtgttcg aaatgaaaga aaaaaagcaa aaattcgttc aaaaaacatg ccctttattc
    19501 aatggttggg tatgattctg ctgattgtat tagctttatt gacgatcatt ccttttttat
    19561 ggatggtttc tgcttcattc aaagcaaata atgaagtgtt aacgattccg attcaatgga
    19621 tccctaaaac ttggcattta gagaattatc tggttatttg gtctcgtatt cctttagtga
    19681 cattttttaa gaatacagct tttttaagcg ttgtgatcac gttgattcag ttgtttactt
    19741 ctagttttgc tgtttacggg ttttctgaac taaattttaa agggcgcgat attttatttt
    19801 tagcatatat cggcacgatt gctgtccctt ggcagtctta tatgatcccg cagttcatta
    19861 tgatgagaaa agtgggttta acagatacgt tgtggtcgtt ggtgctatta caagcgttca
    19921 atgcttttgg tgtgttcttg ttgaagcaat attacagtag tatcccagat tctttatgtg
    19981 agagtgcgcg gatcgatggc ttatcagagt gggaaatcta tcgcaagatc attttacctc
    20041 ttacaaaacc ggcgattgcc agtttgacga tcatcacatt cgttaataca tggaacgatt
    20101 atatggggcc attcatttat ttgtcttcta cggaaaataa aacaatccag ttagggttga
    20161 aaatgtttat tgggctattt gatgcagagt acgcattaat catggctgcc tcagttgttt
    20221 ctgttcttcc tgttgctttt atatttttag cgatgcaaaa atattttgta gaagggatcg
    20281 cttcatcagg aatgaaggaa taggaggtga ttatgaaaga gcagcgtttt gacaaaaatg
    20341 tttatatgga attcttttat tggttgtatc aatcattggc aagtagcctt tgtttttggt
    20401 gtgtcaatat cttgtatata gcagctgcgt tccttttgtc gatagatgga aggaatgtct
    20461 tttcctttat tttttcttta ctttttattg gtccaggttg catcgtgatg ataggtatga
    20521 cagaagaatt agcaaaagaa aaaagttttt caccagtacg tttgttcata aagacgtttc
    20581 gccaattttg gctaaaggga tttttttatt ggctatttgc ttggatagtt agtgtgataa
    20641 tgatttttga ttgtttcttt tttatacgtt tttcttatgg aaaatggttg attccgttgt
    20701 ttgttttact tgcttgtttt acttgcttgt ttatcagttt cctttagcat caactgctgg
    20761 tattttcagg tgagaaatcc agcgtcgaaa ccaaatcaag ttctacggat agcgttctac
    20821 tatacgttga aaaaatggta tgtgagttta ctagattttt tactattgac aagtttgttt
    20881 ctcttctttt tcgtaaagcc ccaatggtgt attctactag gtccttctat cgtatttggt
    20941 ttgatttact tcaacaatcg taaattgatg agaactatgg atctgtagac tttataaatt
    21001 gaaactttca attaagaatg acagtttttt atgtaaaaac tgtcattctt tttcattttt
    21061 tgaaacatat ggctaaacaa atgaaataaa acatgctata cttttgaatg aaacaggttt
    21121 agaaactgta tcacttattt gtgattattt ctaactaata attaaaaata aagcataaaa
    21181 aggacgatgt tcaatggctg aaaaaatgaa acaaggttcg aaatctgtag tagaaagttt
    21241 gattaatcat catgttgatt atgtattcgg tataccaggt gcaaaaatcg atggtgtatt
    21301 taatgaatta gaggatcaag gtcctgaatt aatcgtgact cgtcacgaac aaaatgctgc
    21361 atttatggca caagcaatcg gacgtattac aggagagcca ggagtagtta tcgcaacaag
    21421 tggtcctggt gctagtaact tagctacagg tttggtaaca gcgacagcag aaggtgaccc
    21481 agttttagca attgccggac aagtaaaacg aagcgatcta ttgaagttga cccatcaaag
    21541 catggataat gcagcactgt tccagccaat cacaaaatac agtgcagaaa tccaagatcc
    21601 agaaacaatt tcagaagtga ttgccaatgc ttatcgtatg gctaagagtt caaaaaaagg
    21661 cgcgagcttt atcagtatcc cgcaagatgt agtggatgca cctgtccaag gtaatgtcat
    21721 caagccattg agcgatccta aattaggttc tgcttctgca gatgatatcc gttacctagc
    21781 tgaacgcatt agagaagcaa aactaccggt actattagta ggtatgcgag gatcaagtga
    21841 aaaagaaacg ttagccatca ggcaattagt tgaaaagaca gcattgcctg ttgtagagac
    21901 attccaagca gctggagtga tctcacgtga attagaagcg catttctttg gtcgtgtggg
    21961 tttatttaga aaccaaccag gagatatgct tttgaaacga agtgatctag ttattgcaat
    22021 tggctatgat ccaatcgaat atgaagcaag aaactggaat gcggaaaaag atgcgcggat
    22081 cattgtgatc gatgaagcgc cagctgaaat cgatcctttt atgcagcctg aaagagaatt
    22141 gatcggtgat atctcagcca ctcttgattt attgacaggt tcattagagc ctcaacaagt
    22201 gtcagaagat gcaaaagaat atttagcatc tcttcaagca aaattaacgg agcgggacat
    22261 tgttcaatca aaaggagaag caggtatctt gcatccttta gaagttatta acacgcttca
    22321 gtcaaaagta acagatgata tgactgttac cgtagatgta ggtagtcatt atatttggat
    22381 ggcacgccac tttagaagtt atgaacctcg tcatttgtta ttcagtaatg gtatgcaaac
    22441 gcttggtgtt gcattgccat gggcgatttc tgctgcattg gtgcgtccaa atacacagat
    22501 cgtatcagtt tccggcgatg ggggattttt attctctgcc caggatttag aaacggcggt
    22561 acgtaaaaaa ttgaatatcg ttcatttgat ttggaatgat ggacactaca atatggtaga
    22621 gttccaagaa aaaatgaaat atcagcgggc gtcaggtgta gactttggtc cagttgattt
    22681 tgtgaaatat gcagaagctt ttggcgctaa aggaatccga gctacaagtg tagaggaatt
    22741 agaaaaagct ttagaagaag gatttgcgac agaaggaccc gtaattattg atattccgat
    22801 tgattaccgt gataatgaga aattgggaga aacaatctta ccagatcaat tctattaagg
    22861 aagtgtcgac atgactgaaa aaatcttata ccagcacggg acattaggag ctttgatggc
    22921 cggcttgatg gatggaacag aaacgattgc tcatatatta gaaaaaggaa ctctgggatt
    22981 gggaacattg catgggcttg atggtgaagt catcttttta gatggcgtag cttatcaagg
    23041 aagatcagat ggctcagttg cccaattaga tggaagtgaa cttaccccct atgcagcaat
    23101 tacagatttt acgccagata ctagtttttc tgtctcaaaa actgcagatg gagaacaatt
    23161 gaaaaaagac atattagtaa gagaagccgg ggaaaaccta tttttggcag tgaagataac
    23221 tggattattc aaaaatatgc acatccgtat catgccgaag caagaaaaac cttacagaag
    23281 actagcaaaa atttcagagt ctcaaccaga attccaacaa agcaatgtcc aaggaacatt
    23341 agtcgggttc tttacgcctg aattattcca aggagttgct gcagcgggat ttcacctgca
    23401 ttttatcgat gatacacata catttggcgg acatgtaatg gattttgaag tggctgaagg
    23461 aaaagttgag attagccgca tctcgtccct tgtacagcat tttccagttg atgatccgac
    23521 ttttgttcac gaaacgattg attatgctga tttagcagca gaaatccaac aagcagaata
    23581 gaaaaagaca gcgagatgcg ttcaaggtga ttaacctgac gtatctcgtt tttttgagga
    23641 gaaaacctag cgtttacaaa aaaactgagc tataatcttc tccatagatc aaaaaggagc
    23701 ggtttttatc atgtggacag aaaaacagaa aaaagatgca tacgaaattt tattattaca
    23761 gcaacaagga ttattagaag cagaagataa ctggttagca aatttagcaa attcatctgc
    23821 attgttgaat gagactttgc ctgagactgt ctttgcagga tattatttat tcgatggtaa
    23881 tgaattgatt ttaggaccgt tccaagggcg agtttcttgc acacgtatca agatgggaaa
    23941 aggagtttgt ggagaatcag ctgaaaaaca agaaacattg atcgttgatg atgtcaaaac
    24001 ccatgcaaat tacatttcct gcgattcagc cgcacaatca gaaatcgtag tgccaatggt
    24061 aaaagaaggt aatttaatcg gtgttttaga tatcgattgc ggagtaacga aagggtatga
    24121 tgaaatcgat caagcatttc tagaaaaata tgtagatctt ttattagcaa attttaatat
    24181 aaatgctttt tcatttatta ataactaaaa tcgtctattg aaacaaaaaa gaagagaaat
    24241 caagaataaa aagagccgga aagacagtgg gcatttctgt ttttcggttt ttttattttt
    24301 ttataaaatc acaagtgtta ttgattttca taatcatatt ttaatgttat tatcatttta
    24361 aataatgaaa aaacattaag gatggatgaa atatgaataa cgaaactatc attttaggaa
    24421 aaaaatatat ttgccaacct attggcttga aacaaaaagt gattggagaa gtaatcagta
    24481 aattagaaaa ttgtgttgtg atctgcgtag acgaatatca tgacgtcgat cgcaatgaaa
    24541 tattagaaaa atgcggaaaa gtagtggcaa aatatactca cgtttatgag ttggaaacaa
    24601 cagaatatct atataactat agcaacaaat atgaacatac tttagtttca taaagattta
    24661 aataaagtgc cttcaaaagc gagaagacca agcttttgaa ggcactttat ttagttggca
    24721 gtgattgttc atgatttttg atttattcaa aaggttcgcc tagagaatct gtctctgtaa
    24781 cgaggcctgg tggagaaact cgataaaaac ctatactgcc agaactgtcc tattttcgga
    24841 tagacaaact agtagtttta aaaagatcat ccgatccaat catttcaaaa aatgcaaaac
    24901 tgatatcagg atcatttagc tcaggcttgt tatcgtaaag catttgtaaa ggttgcattt
    24961 agttaggagt aaacgcgaaa ctatcatcat tatttgctga agaagatgaa gaagctaatt
    25021 cagagtttgt ttgaaataaa ggagacgtgc tgacagtttc tttaccctgt tttttgcaga
    25081 ggtagaaact tttatatcgg aagtttctac cggagaattt ttctcatttg tagaaattgt
    25141 tgaatcgata ctattcgaaa tttgcgtatc tgaaggtatg ttctttgatg cagaagtccc
    25201 acaagcagta agtaacaata aatttgataa actgataaac agaattcttt tcattatgta
    25261 cacactcctt atagtatcgt ttagcataca cctatgctca gtattaggca aaatcaatgc
    25321 aaaactatca agtcttttat agtatctatt agtcttagta atacatatat ggagagggtg
    25381 atatcccagt tcaaaccaga tacattggtt accatctgta ttaacgatta tgcctctaaa
    25441 cgcttttttt atgaaaaaat ttataagtga aaacgtaggt atgatattat ctagaggtaa
    25501 tttttttggg tatttaaata gttgctagga ggatagcttt tggattttta cagtaatttt
    25561 atcttaataa tagcaatatt attacttcta aatatttggt tttttgataa gagtagaaat
    25621 gcagggatag gttttagaac caaacgaagt acgtcttctg aaaaaaagtg ggtatattca
    25681 cagactattt tttacggagg ggttatttca atcagtttac tttcttcaac actttattct
    25741 tttaatgtta tagacgtttc catgtcaaat tttatatcta ttattggaat ccttatttct
    25801 gcaattatta cacaactact tcttgtgttt gaagagaagt caaaaaataa ttaaagcaat
    25861 tatgcctttt agtacaggaa ttattacttc ttacaaatgt atagcgaatg tgaagcctgc
    25921 tgttttaatt gttctagaaa aacttttgag tgaacaatta ttttatgtta ggatgaatta
    25981 atttctctag agtttata
//
LOCUS       DAZBSA010000034        25642 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000034 DAZBSA010000000
VERSION     DAZBSA010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.034"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            70..2130
                     /locus_tag="ACHGMT_001274"
     CDS             70..2130
                     /locus_tag="ACHGMT_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFN6835025.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2213..2782
                     /locus_tag="ACHGMT_001275"
     CDS             2213..2782
                     /locus_tag="ACHGMT_001275"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6835026.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKSDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(2957..3763)
                     /locus_tag="ACHGMT_001276"
     CDS             complement(2957..3763)
                     /locus_tag="ACHGMT_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835027.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3901..5415
                     /locus_tag="ACHGMT_001277"
     CDS             3901..5415
                     /locus_tag="ACHGMT_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFN6835028.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5818..6801)
                     /locus_tag="ACHGMT_001278"
     CDS             complement(5818..6801)
                     /locus_tag="ACHGMT_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFN6835029.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6806..7453)
                     /locus_tag="ACHGMT_001279"
     CDS             complement(6806..7453)
                     /locus_tag="ACHGMT_001279"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFN6835030.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7538..8170)
                     /locus_tag="ACHGMT_001280"
     CDS             complement(7538..8170)
                     /locus_tag="ACHGMT_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFN6835031.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8186..8737)
                     /gene="coaC"
                     /locus_tag="ACHGMT_001281"
     CDS             complement(8186..8737)
                     /gene="coaC"
                     /locus_tag="ACHGMT_001281"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFN6835032.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8734..9471)
                     /locus_tag="ACHGMT_001282"
     CDS             complement(8734..9471)
                     /locus_tag="ACHGMT_001282"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFN6835033.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9468..10457)
                     /locus_tag="ACHGMT_001283"
     CDS             complement(9468..10457)
                     /locus_tag="ACHGMT_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFN6835034.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10715..11257
                     /locus_tag="ACHGMT_001284"
     CDS             10715..11257
                     /locus_tag="ACHGMT_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFN6835035.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11274..12359
                     /locus_tag="ACHGMT_001285"
     CDS             11274..12359
                     /locus_tag="ACHGMT_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6835036.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12371..13177
                     /locus_tag="ACHGMT_001286"
     CDS             12371..13177
                     /locus_tag="ACHGMT_001286"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6835037.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13178..14005
                     /locus_tag="ACHGMT_001287"
     CDS             13178..14005
                     /locus_tag="ACHGMT_001287"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6835038.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14002..15075
                     /locus_tag="ACHGMT_001288"
     CDS             14002..15075
                     /locus_tag="ACHGMT_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN6835039.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15503..18199
                     /locus_tag="ACHGMT_001289"
                     /pseudo
     CDS             15503..18199
                     /locus_tag="ACHGMT_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
     gene            18264..18764
                     /locus_tag="ACHGMT_001290"
     CDS             18264..18764
                     /locus_tag="ACHGMT_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN6835040.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            18776..19270
                     /locus_tag="ACHGMT_001291"
     CDS             18776..19270
                     /locus_tag="ACHGMT_001291"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN6835041.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19291..20100
                     /locus_tag="ACHGMT_001292"
     CDS             19291..20100
                     /locus_tag="ACHGMT_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFN6835042.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20097..20918
                     /locus_tag="ACHGMT_001293"
     CDS             20097..20918
                     /locus_tag="ACHGMT_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN6835043.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            20969..23068
                     /locus_tag="ACHGMT_001294"
     CDS             20969..23068
                     /locus_tag="ACHGMT_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFN6835044.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23065..24183
                     /locus_tag="ACHGMT_001295"
     CDS             23065..24183
                     /locus_tag="ACHGMT_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFN6835045.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24222..25100
                     /locus_tag="ACHGMT_001296"
     CDS             24222..25100
                     /locus_tag="ACHGMT_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFN6835046.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25246..25443
                     /locus_tag="ACHGMT_001297"
     CDS             25246..25443
                     /locus_tag="ACHGMT_001297"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFN6835047.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            25427..>25642
                     /locus_tag="ACHGMT_001298"
     CDS             25427..>25642
                     /locus_tag="ACHGMT_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835048.1"
                     /translation="MNFFGDRFFQPYKINRNSIIESCDYKKIARKKKKVSIHFKYFIP
                     ISIAKKVEILYLPLTLASLANVVSILID"
BASE COUNT         8105 a         4458 c         5425 g         7654 t
ORIGIN      
        1 atataatgaa atcatcttat gaaaattact acttcatgtt tagacaagag tagggaggac
       61 taagaataaa tgtgttcaaa aagacgttca aaacaactaa ataaacacgc ttgtcgttta
      121 gataaggaac ggtcgtgttc tgaaaaacac tacgttaagt ttatgatgcg cacgatgctg
      181 atcagtttat ctgtaggtgt ctatatgcat gtacccgttc aggcaacgac acaaaccatc
      241 aaggtcgtac gtacctctgt ttctgagacc actgattccc ctatcactat tactcatact
      301 ttcactgatg caaatggaaa acagctactt cctgcattga cttcctataa taaagtcagt
      361 tatcctccaa cgataaacca ctactggtta attcctaaca aaacattatg gacatcggaa
      421 accggaagca gtgtgccgct ttctatcttt tccgatatga ctgatcctac aaaaggtgat
      481 cagttaaaac agatcactga tattttgaat catgctttga cagatataaa ggacggtgga
      541 agcgcacaag tttcttatgt atatgaaaaa gattattcac agtttgaaag aaagaaaatc
      601 gatgttctgg cagggacaaa agtttcgata tctgatctgg tagaaaggct gaagaacgca
      661 gatggaacac aaggagatat gtccaaagta atttcaaaca atgggaatat cgtggatacg
      721 actaatacag gaacatctac tatttccttg tcctattttg atccaattgc tttaaaaaca
      781 atgactgcat ctgctgtgat ccatgttcgt gctgatcaaa cagctatcaa aggaaaagat
      841 cttcacttaa gaacaggaga gagttacgcc cctaaagatc ttgttgaata tgtaacaaaa
      901 agcgatggaa cttcaggaaa taaagaagaa atcaaaatga acgggacagt agatactaca
      961 actccgggag aatatcccgt tgtattaact tatatagact cgaaaacatc aaatgtggca
     1021 gaaacaacag ctgtagttta tgtagaagca tctgctgctt catcgactgt catcactcat
     1081 tttatagata gtcaaacaaa taaagaactt caagcagaaa cacatatgag tagtgtaaat
     1141 cgtattcctg atttgcgcct acctgctgaa ttttctgatc gagtgccaaa tatagattta
     1201 tcgacttatg aacaaaatgg gaaagtcact tctatttctg attggatgca agcaaacgga
     1261 ttagacagaa aaagtcactg gtcggagatc cttgaagtac ttcactcaga actgatgaat
     1321 aataggaagc aatcaggagg ggaaattacc tttacttatg tttatatacc agacaatcgt
     1381 tcattagaag gtcatgatct tattgttcat ccaaacgaaa aaataaacaa agaagagttg
     1441 atccaatcag caacaaattc cttaggtaaa cccgtaccgt taaaggatgt atcggttaca
     1501 atcaatgaag taccattgga ggatggttat ttgaccaaac aaacaggtga ttttacgatt
     1561 atgtatagtt atcatgaccc gtattcactc caagaactcc atgctcagtc actattacat
     1621 gttttaccat taaacaaaca gatagatcaa aaagaacaaa aaagacaaga agaaagcttg
     1681 tcagaaaaaa aacaacaaat ggctcatgca gtttctccaa cagggaaagt aaaacaaaca
     1741 agaagcctag agttagcaag aatatctaga aaaatctcag aatctaataa aacgataagg
     1801 caagaagctc atttggcccc acaagtttct ggattggaaa atgaaatttt agagcctaaa
     1861 ataaaacaac aatcagaaaa agaatttgtt gctaaacagt cggaagatca gaaggaatca
     1921 gatgaccaga aggaaacgaa gaaaaaacgc ttgtcaacta ttgattccac cgatgagcat
     1981 ctgcagccgc aagtaccact tcctagtccg ggaacacccg cacctggtgg aggtattcct
     2041 tcttctgctt taggagattt catgcgaggt atggcaggaa catgggtata cgctaatcga
     2101 aatgaatttg acgcagatga ctggttctga tttatatctc tatctgttat agtcaaataa
     2161 gtagctgagt catatgttat ttggcataat ggagacgagg agggatagaa aaatgttaga
     2221 taaaacgtta ccttacgcag aaatctggat gacaagacca cttcaacaaa atgttccaat
     2281 cacgccttta gcggaaggtt ttgaatttcg attttatcaa aaagaagatg aatctgcttg
     2341 ggcggagatt gagacttccg ttggggaatt tcaaacgaca gatgaagcac ttgagtattt
     2401 tcaacaaaac tttgctcctt atcctgaaga acttgaaaga aggatgtttt ttgtagagac
     2461 aaaaaaagga gaaaaagtag caacctgtac cgcttggcga aaaaaatcaa gagagcaagt
     2521 gtatccggtt tttcattggt tagcagtgaa atcagattac caaggaaaag ggctcgcaaa
     2581 agcattgact gccagagtct tgcaagaatt tcctaccctc catacggacg gaccaatcta
     2641 tcttcacaca cagacatgga gccatcaggc gattgctctt tataaaaaaa tgggttttac
     2701 ttttattgct gaaaatctag acggcacacc taatccagat tatgaaaaag ttatgcaggt
     2761 tttaagtaaa ttagataaat aacgaaatgg tgaaataagt cctcagtatt gggataaaaa
     2821 acgaaatagt cattcctcat atcttacttt tttgagttaa tactatcgga taactaatgg
     2881 catgccatac attcaaaaaa gaaggggcag tgacaaaagt attgtcactg ccccttctct
     2941 tgttttttag catgttttat cgtttgattt ttttaggaat aaaattcttg atgaacgtaa
     3001 atttttggta atcgtttcga ctatagaggt aaccaaatac actgaaaaca actgctccaa
     3061 taaaattaac aaataaatcc ttcatagtat ccatgattcc cacgtctaag taaccatttt
     3121 ggatgattgt tttatctgtt ttaccatctg cattttgact ttcaatgatt gtccgagtga
     3181 tatctggaac atgatagaca gtgtttccgt ctttacttaa ctccacacta ttgatttgct
     3241 ggatgatcgt atctttttgc atatcgaatt gcagtaattt atctgctgaa aattccacaa
     3301 actcccacat aacaccaact gtcatcgaaa aacagaagga aacaactgct agtaataaag
     3361 gagtcaagtt catggttttt gtatttttat tgagcaaata aacaagggaa aagccgacac
     3421 ccccagctaa aaatccgctg aatgcatgta gaaaagtatc ccacattgaa aaatagccat
     3481 aaaaatcact taactctcct aaaactgttg aactaaaaat aaaagataac aaaatgattt
     3541 ctagcatatt tggaatctgt atctttaata tcttttccag aaaataaggg atttcaaata
     3601 aaattaatgt cagtaataaa agcattgtat ttccccagtg ttggttccag acctgatcca
     3661 ctagcataat aacgacgatg atacgtaata aaatatagat tccgtttgtg attctacctg
     3721 cagtttttgt actgttcgta aaaaaggttt tcaaatgatt cataaaataa ctccttcatg
     3781 tatatcctta attatactaa gaagcgtgtg tttaccaaag ctatatgact taaagaaaaa
     3841 cagagttgat attgtgaaaa aaatcactta atatagtata aggagaaaag ggggatacaa
     3901 atgaaaaaat tagttgctgg tttattaaca gccgttgcac ttcttgctat gagcgcatgc
     3961 ggcgcagatc agaaaaatac agcgcagaca agttctagta cgaaggaatc tgccgaagta
     4021 tcatcaggcg cttctgaaca agtatataca gatccaaaag aaatgaaaga atcttatgat
     4081 gtcgtgatta ttggagctgg tggtgcaggg atggcagcag ctatcgaagc aaaagatgct
     4141 gggttgaatc cagtaatttt ggaaaagatg cctgttgccg gaggaaacac atctaaatca
     4201 tcaagcggta tgaatgcttc agaaacaaaa tttcaaaaag aagaaggaat caaagactcg
     4261 aatgatgcct tctatgaaga aacgctaaaa ggtggtggag gaacgaatga taaagaaatg
     4321 cttcgatact ttgtcgatca ctctgctgat gcggtcgatt ggctagatca aaacggtatc
     4381 aaactggaca acttaacgat cactggaggg atgagtgaaa agaggacaca tcgtccgtct
     4441 gatggctcgg ctattggtgg atacttagtg gaggggttat tgaataacgt ccatgagcgg
     4501 gaaatcccta tttttgtcaa cgcagatgtg acagacatta agaaaaatgg ggaaacggtc
     4561 aatgaagtga ctgtacatgt tcaaggagaa gaaccaaaag aggtcaccgg aaaagcaatc
     4621 gtagttacca caggcggttt tggtgccagc aaagaattta ttgaaaaata tcgtcccgat
     4681 ttagctgatt atattacgac aaatcaagaa ggatcaactg gagatggtat caccatgatt
     4741 gaaaaattag gtggacaaac cgttgatatg gataaaatac aaattcatcc aactgtgcag
     4801 caggatgagg gggtcttgat tggtgaggtt gttcgcggag aaggagcaat cttggtcgat
     4861 caagagggga aaagatttgt caatgaaatg gatacaaggg ataaagtatc tgcagcaatt
     4921 acagcattgc ctgaaaaatc agcttatttg atttttgacc aaggcgtgcg tgatcgtgcc
     4981 acagctattg aattttatga ccaaaaagga tatgtcacaa ctggaaagac aatggaagaa
     5041 ttagcagaaa aaatcgattt accaaaagaa acactagcaa aaacagttga tggatggaat
     5101 acggatgttg cggagaaaaa agatgctcaa ttcaaccgtg ccacagccat ggaacatgac
     5161 ttatcgtctc cgaattatta tgcgatcaaa atcgcaccag gaatccatta cacgatgggc
     5221 ggtgtgaaaa tcaatacgaa cactgaagta ttagataaag aaaaccagcc aattaggggg
     5281 ttatatgctg ctggtgaagt cacaggtggc ttacatggaa acaaccggat cggtggaaat
     5341 tcagttgcgg agatcattat ttttgggcga caagcgggca tacaagcagc tcgatttgct
     5401 tcggaaaatg aatgaaatag aacgtaaata aaaagaggtt gtgaaatcag cgttttgagc
     5461 taataccgag ggtcagctga ttgaacactg tttagtgaag aggttgtgaa aaagctgccc
     5521 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggttgg
     5581 acttattacc gcagatgcaa gcttttgaac accgtttatt cggaaaaaga gcgtgggaca
     5641 aaagtaaaga acacttttgt ctcacgctct aaacacgtat aaacggcgga agcagaagaa
     5701 attccttcgg aaataagctg aaattcacaa aaatttgaaa agcaattttc gtgaatttct
     5761 tcttatttct tggagttaaa cgcttctgtc ccagcctctt ttttgatcag catgatttta
     5821 ttcagttatc ttcaaaatga tttttccgat gtttttgttt gcttccatat attcatgcgc
     5881 ttgtttgact gcgctgaatt ggaatgtacg gtcaatgatt ggccgaatct tgccattttc
     5941 tagaaaaggc ccagcttttt gcataaaagt ttgtgcgagc tctgctttat actgatcact
     6001 tctaggtgtc aataatgtac caagcagttg gacacgtttc ccaatcaata caccaaggtc
     6061 tatctcaggc acgatggtcc cacctagcat accaatcaag acccaacgtc catcaacggc
     6121 tatagaagct aaattctttt cccagtaaga agcgccgatg aaatccaaaa taacatcgac
     6181 tccccgacct tcagtgattt ttcgtacctc ttcttgaaag tcttgttttt tatagttgat
     6241 caattcatct gcaccaagtt ctttacaaaa ggcgagtttt tcttctgatc ctgcagtaac
     6301 gatgattttc gcgtgagaaa gctgtctagc taattggatc gctgctgttc ccacaccact
     6361 tgctcctgca tggatcaaga cagtttcgcc ttcttgtaat tttccaagcc agtaaagtgt
     6421 ttgataagca gtcaaaaaaa cttctgagat tccagcagca gaaacatagt ctagcgtatc
     6481 agggagaagg attgcacgat cggcaggcat cacagcatat tcggcataac cacctagatt
     6541 gactaatccg tatacttttg tcccaggagt aaaagtggag atatcacttt tattctcaac
     6601 tacctctcca gctacttcga ctcccaaaat agggtaggga ggtttcaaag agactgattc
     6661 tcttttcata atatctgttc gattgacagc tgctgtatgg actttgatca gtaattcgcc
     6721 ttgttttggt ttaggaacag gcacttcaat ttgttctaat acatctacag aaccaggatg
     6781 cttgattgaa atggcacgca tggatctatt cctcctttgc ccattcttgg aatgctgtat
     6841 tcaactcttt gctaccttca gaaagtacta gagtcgtacg agaaatatcc gtttcttcca
     6901 ctagagcggc aagtacagca gcaactgtag agcgagtgat cgtttttgat gccttctcat
     6961 taggcttagt taattgaatg gaagtaactt cttcatcgtt agtcaatgtg actggttgaa
     7021 gaatgacaaa atctaagtta gttcgtttca gccattcatc tgcataatgt ttagtaatat
     7081 aataatctgt catggactca ggccattttg ctggttcatc tgcataaaca gcactgacca
     7141 tcagatagcg agagacatgc gcattttcag ctgcgatcac tgttttgatt gcgccatcca
     7201 agtctacttg cagaagattc tttccttgag agccggcagc aaaaatcaat acatcgatgc
     7261 ctttgaatgc ttcggtcatt ttttccacgg accaagttaa atcaaaagag acatacgaaa
     7321 cattctcttt ttttactaat gtttgggaag caacatctct cacccctgca aaaatttcat
     7381 gggaacttgg agctaaatct tttatcaggt gtcggccaat ttgtccgttt gcacctacaa
     7441 caaaaatttt catttggatc atcctttcga cgtcaaatct ttccattcaa tagtaacacc
     7501 aaaagagaaa aaaatcagag atcggctacg atgactatca gttagtaaga gaagggtgct
     7561 tgaagtatct taacaagaca ttgcatactg ctgcagtcgc aagacctgcg acgattcctt
     7621 caggtatacc acttgtaaaa ataaccgcta gaatagctga ataaacagca tttaaatttg
     7681 ttccgatgac tttggcataa tcttgctgga ataaaaagta gatcatattc atcactaaaa
     7741 tcgtattggt caatgaaccg gaaagtccag ctaagaataa agaaacggaa tctgcttttt
     7801 gtttcattag tttgcgcaga cctttataaa cgaaataagg aaccacccca atcaagatgc
     7861 gaggaataaa cgcgatcagc aaggctttcc agcttccatg ttctgttccg atgactggga
     7921 taaacggaga aaagacaaaa gacaaaggtg tttggataac tgtgctacgg atcatgctga
     7981 tcagtccaaa tgtaccgcca agaaaagcac ctagtcttgg tcctaaaacg atcgaagcaa
     8041 tgattacagg aatatgcatg gttgttgcat tgattggacc gatagggata aaacctaaag
     8101 gcgtaacagc taataaaatc aaaatagcta aaaacatcgc agttaatgta aagttttttg
     8161 tatttttcat aagtactcct tttttctaag catttctttc attaagagtc agcatgacct
     8221 gttcgatgat cgtctgaata tcagctaagg caccttttcc aaaatctcca caagctaaaa
     8281 gagattctct tggttcaatt tcctgatagc cgacagatct caatgtttcc agattacgct
     8341 gagtgatcgg gttttgatac atattcgtgt tcatggcagg ggcaatcaat tttggtgttc
     8401 ccactggaag cgccattgcg actgttgaaa gaagatcgtc tgcaatgccg tttgctagct
     8461 tgccgataat gtttgctgta gcaggcgcga tgagaaacaa gtcagcttgt tttgctagtt
     8521 cgatatgatt gattacctgc ggatttgatt cttgcatgac gtctgtatgg actgcgcgct
     8581 tcgacagaga ttgcaaggtt aatggcgtga taaaatgtgt gctgctttta gacataatca
     8641 catctacctg ggcacctaat ttgacgaatt gacttgtaag atcagcgctt ttgtaagcag
     8701 aaatacttcc agtaacgcct agtaagatct ttttcatttt gattcctcca gtctaatttt
     8761 ctcagtaatc aatgcagcaa tctcagattt actttgagct tcttccactg ttccatcttt
     8821 agataagaga tagccgtggt gtgtctcgcc atggatttcc gtcaaatcat ttgcgaaaat
     8881 aaaatcagtt ttgttattta tcagactatt ttttgcgact gccagaagag attcctttgg
     8941 tacatctact aatagtttga aaccaatgat ttttgctttt ggctgccatt cacgtagata
     9001 agaaatgatt tttggtgttt tcttcaaaac gagcacaaga tgctcggtgt tagaagagat
     9061 ctttgaaaca gtggtatctg tctgttcatt ttccgcaaac caattgtcca gttcttcttg
     9121 cgttgaagaa gtgggtaggt tttttgccag ctgttcttct gaaaaactaa aagcaggagt
     9181 aaaatcactg actgccatac tgtggataat cgcatcgtag tgttcttttt tcgttagttc
     9241 ttctaattgt aaaaataagt ccagtgcaga ttcgatcgta taaatcgtta tattggagtg
     9301 tcttttcggt ttcagtgctt gtttcgtagt cacgtaatcg attgtggtat ccggattggc
     9361 agctaaataa tcagctaatg cttgccctaa tcgtcctgtc gaatgatttg taatagcacg
     9421 tacttgatcg attttttcag tcgttcctcc agctgtgatt agaatcttca tttatggtca
     9481 cctacattta ctagtgttct tccttggtgt gttccagcaa gtagtttaga tgctgtttca
     9541 ggcaattgat ccagagtaat ctcttggacg accagttcat ctgcaatctg caaatccgcc
     9601 atctgttgcc ataaagaaag acgttgatct attggtacat tgacagaatc aattccaatc
     9661 aactggatgt ttcgcagaat aaaaggcaag acagtcgcgt ttagtttgat tcccccagca
     9721 ttgccacata aagctactgc accgttataa gagatcaatg gaagaagact gcttagctgc
     9781 tcgccaccta ctgtgtcgat gacatagtcg atttgctgtt ttcctaaagc ttttgttgtc
     9841 tctggcagaa attcatcagg tcgtacgact tgcaaagcac ctagactttt tagccatgtg
     9901 acagactctt cttttctgga aaaggcaatg attgactgga atcctagttt atgtaacagg
     9961 gctatcgctg tactgcctac accgccagaa gcaccagtaa cgacaacttt tttatcggag
    10021 gtcatacctt gacggattaa agcgttcaca cacaacatag cagtaaaccc tgctgtacca
    10081 aggatcatcg cttgacgcag cgtcatattt tttggtagag gaactaacca gtctcctggt
    10141 actttttgat attggctata accgcctgga tgggtgacgc caagcccaaa accagtagca
    10201 agtacaagat cttcttttga aaaacgtgga ttgttacttt ggacgacagt tccggataaa
    10261 tcaatccctg gagtcatcgg atatgttcgt atcacctgac cagactcgct cattgccaat
    10321 gcatctttat aattgacatc agaataggca atttttacca agacttcatt ttcagccaac
    10381 tcagtaaatg gcagatcact gattgctgta tggaaaacag gttcctttat tacctgaaaa
    10441 gatcgaaaag cgttcattcg tgagacactc cttttaaaat tttcttttat cataccataa
    10501 agcctactct tttattcttg ttcttccgta aatttagaca aaaattaggt gaaaaataaa
    10561 gaatcaaaca actagaattt attccaaaag ataaagaaat attcagcaaa aaaaatttta
    10621 caaagggttg cctttttcaa taaatttcgt atactaatac gagtggtagt aaacaggaag
    10681 tttactatta gaaaacttcg attcggagga aattatggaa attggtgaga agttaagaaa
    10741 tttgcgcatc caaaagaatt tgacacaaga agaactaggc gaacgcacag atttgtcaaa
    10801 agggtatatt tcccagttgg aacgagattt aagttcacca tcaatggaaa cattcttttc
    10861 tattcttgaa gtcttaggtg tgactccaga agaatttttt catcaagaag cagcgaatct
    10921 gcaagtggtt tattcaaaag aagatcatac ggtttatttc gatgaagaaa acgggtatga
    10981 attggaatgg ctcgtggctg attcgaatga aaaagaaatg gagcctgtct tgctgacttt
    11041 tgaaggtgca ggagaatata agacgtttga accttcatta tctgaaacgt ttatttttgt
    11101 attagaaggg gaattagagt taagcttagg agagacggtc tatgtggcaa aagcaggaca
    11161 gtcgatctat tatcaagcga caaaacaaca tcaattaagg aatcatctca aaaaacgaac
    11221 aaaagtattg attgttgcaa cgcaatcata tttgtaatat agggggtaaa aacgtgggga
    11281 aaccaatcat ttcatttgat caagtagtga aacgctatga tgatgaaaca gtattaaaaa
    11341 aagtaagttt tgagatcgaa caaggaaaat tttatacact gcttggtcct tccggctgtg
    11401 gtaaaacaac gatcctacgt attatagctg gattcaccga agctacagaa ggggacatct
    11461 attttgaagg gaaacggttg aatgatttgc cagctaataa gcgacaagta aatacagtct
    11521 tccaagatta tgcacttttt cctcatatga atgtttttga taacgttgct tttggattga
    11581 agatcaagaa aatgtccaaa tcagatatcg aaaagaaggt aaaagatgct ttgcgtatgg
    11641 tccagcttcc tggatacgaa aaacgggaga ttagtgaaat gtctggtggt caaagacagc
    11701 gtgtagcgat tgcacgagca attgtaaatg aaccgaaagt attgctatta gatgaaccgt
    11761 tatcggcgct tgatttgaaa ctacgtacag atatgcagta tgaactgcgg gatctgcaac
    11821 aacgattagg aatcacgttt atttttgtta ctcatgacca agaagaagcg ttggcaatga
    11881 gtgatgagat ttttgtcatg aataaaggga aaatcgtcca aagtggtaca cccgtagata
    11941 tttatgatga gccaatcaat catttcgttg ctgattttgt tggggagagc aatatcgtgg
    12001 acggtgtaat gatcgaagat aatctagttg agtttgttgg taagcaattt gaatgtgtcg
    12061 atggcgggat gcgtctaaat gaaccagttg aagttgtttt acgcccagag gatttgacga
    12121 tcacgacacc agataaagga aaactagtcg taacggtcga tactcagctg tttcgtggtg
    12181 tccattatga aatcatctgt tatgatgaac agcaaaatga atggatggtc cattcaacga
    12241 aaaaagctaa agaaggaagc aaagtaggat tggcctttga gccagaagat atccatgtca
    12301 tgcgtttcaa cgagtctgaa gaagaattcg atgcacgtct tgacagctac gaagaatagg
    12361 ggggacaaga atgaaaacaa tgcgcaggat ctattccgtc ccttatattt tgtggctcgg
    12421 tttattcgtg atcgcgcctg tattaatgat catttatcaa tctttttttg atatgaatgg
    12481 gcaattcacg ctgaataatt atctatctta ttttacgtcg gggacatcgc tttctatgac
    12541 gatcaattct gtatggtatg catttttgat cacgtttttc acgctgatca tcagttaccc
    12601 gactgcttat tttttaacaa aactgaagca caagcaatta tggctgatgc tgattatttt
    12661 gcctacatgg gtgaatctgc tgcttaaagc ttacgcattt attgggattt tcagtgtgca
    12721 cggcagcgtg aatcatttct ttgattttat cgggatcggg ccgaaacaga tattattcac
    12781 agacttcagt tttctgttcg tggcaacgta tatcgagatt ccatttatga ttatgccaat
    12841 tttcaatgca ttggaagaga tgaatccttc tttgatcagt gctagtagag atttaggcgc
    12901 gaatagcttt gagaccttcc gacgagtgat ttttccgtta tcattaaatg gtgtgaaaag
    12961 tggtgtccaa gcagtattta ttccttctct ttctttattc atgctgactc gtctgattgg
    13021 cggaaatcgg gtgatcactt taggaacggc tatcgaacag catttcttgg ttacacaaaa
    13081 ctgggggatg ggttctacaa tcggtgtgat tttaatcatt gctatgttca tcgtgatgct
    13141 cctgacaggc gagaagaaaa aaggaggtcg ctcataaatg aaaaaattta aatggtcgta
    13201 tctttattta gggatcgtct ttttattcct atatgcaccg atcttctatc tgatttttta
    13261 ttccttcaat gaaggaggaa cgatgaacag ctttacagga tttacttggg aaaattatga
    13321 agctgttttt gaagatacac gtttgatcat tatcgtattg aatacgttca tgctggcttt
    13381 cttatctgca ttgatcgcca cgatcatcgg gacatttggg gctatgggaa tctattacac
    13441 gaaaaaacga cgtgcacgga cagcgttgtt gagcatgaat aacatcttat tagtctctcc
    13501 tgatgttatt atcggtgcta gtttcttgat tttctttaca ctactaggat tcggattagg
    13561 ttttcagagt gtattgcttt cccatattgc tttcagcatt ccaatcgtcg tcttgatggt
    13621 cttgccaaaa ttgcaggaaa tgaacgattc aatggtcgat gcggctagag accttggtgc
    13681 taataatttc caagtagtca aaaatatcat tttgccttat ctttctccag gaatcatagc
    13741 tggatatttt atggcattta cctattctct tgatgatttt gcggttacat ttttcgtcac
    13801 aggaaatggc tttacgacgc tctcagtaga gatctattct cgtgcaagac aaggaatcag
    13861 tttagaaatc aatgcgctaa gtgcgttagt tttcttgttc tccatgatat tggtcattgg
    13921 atactacttt attagtaaag aaaaccgtcc aaaacggctg aagaaaaaca gaagagtacg
    13981 cgagggggtg gcacggctgc gatgaaaaaa ttacaatctc tgtttttagg tattctcatc
    14041 attattgcgg tgctgctgct tggtgttcgt caacttgaaa aaacaagtgg catgagtgga
    14101 gcaaaggtac ttaccatcta taactgggga gattatatcg atcctagttt gatcacgaaa
    14161 tttgaaaaag aatatggata caaagtgaat tatgaaacat ttgattctaa tgaagcgatg
    14221 ttcacaaaaa tccagcaagg tgggacaaac tacgacatcg ctataccaag tgaatatatg
    14281 atccaaaaaa tgatcaaaga aaaactagta cttccattag atcattcgaa gatcaaagga
    14341 ttaaataata ttgatccacg atttttagac ttggattttg atccagataa tcagtattcg
    14401 attccatatt tttggggaac tttagggatc gtatataacg ataaattctt tgatgcaaat
    14461 caaattaagc attgggatga tttgtggaag ccggaattaa aagacagttt gatgctgatt
    14521 gacggtgcac gagaagttat ggggctttct ttgaatagtt taggctattc tttgaatagt
    14581 aagaatatgg cacagctgac agaagcagca aataaattga atcatttaac accaaatgtc
    14641 aaagcaatcg tggcagatga gatcaaaatg tatatgatca atgaagaagc ctccgtggca
    14701 gtgacatttt ctggcgaagc tgccgatatg atgtgggaaa atgaacattt acattatgtg
    14761 atcccaccag aaggatccaa tctttggttt gataatatcg ttatgccaaa aaccgtgaaa
    14821 aataaagaag gtgcctatga tttcatcaac tttatgctag aaccggaaaa tgcggcacaa
    14881 aatgcagagt atattggtta ttctacacca aatgaagaag caaaagcgtt attgccaaaa
    14941 gacatttcgt cggacgaaca attctatcca tctgatgaca cgatctccca tcttgaagtc
    15001 tacaaagatc taggatcgaa atacctaggc atctataatg acctattctt agaattcaag
    15061 atgtatagga gataatggca aaactcagta agaatgggga cttatcaacg ttctaaagag
    15121 ctaaaaatcg tcctttttct atctgagttt tccaatcttc taccaaaagc ggatgaaacg
    15181 ccgctttttt tgtgttattt tattttttct accacgtttt tctaccagag aaaagtggac
    15241 aaaaccattg taaaataggg cccgagacgg gctagtaaat ctaagattga aaaagttaca
    15301 gaaggataaa gtaattggaa actatgcaaa tatacttaac ccttctgtaa tctattaata
    15361 aaactaatat atgaatccag ttcaatattt gtagctatga aaaaacataa aaatcatatt
    15421 agataaaaac taaattggta gataaattat tttgtcagat atctgaggca aatattcgac
    15481 tatttgtgtt tcaaagtaaa aattggaata caaatataga atagacatat tgcagccgat
    15541 agtttattat gcttttagac acattaaact ggaggaaaaa atgaaaaaga gcgctaaaga
    15601 tcgagttttt gatgagctga tacgactaac agggaatatg tcttctgtca ctgccgagaa
    15661 aatttcgaag aatctaggta tctctcgtca gaatgccagc cattatttga cgagattagt
    15721 ggaagataaa aaggtcgaga aattaccggg aaagcctgtt ttgtggaagc ctttagatga
    15781 atatgcggtt gtagataata cggagcaaat aaacgaagcc tttcattctg tagttggtca
    15841 tgacggaagt ctgagagagg tcattcaaaa atgtatagcc gctgtcaaat atccgcctaa
    15901 cggactcagt gtattaataa atggagccac gggtgttgga aaaagttttt tagccacaaa
    15961 aatttttgaa tacgctatcc atgagcaaat tattgaaaaa gatgcaccat ttgctatctt
    16021 aaactgtgcc gattatgcgg acaatcccga actgctttcg gcaacactgt ttggatacaa
    16081 gaagggggcc tttacggggg ctgaaaagga taccgatggt ttattggcaa cggccaacaa
    16141 cggctatctt tttctcgatg aggtccatcg cctatcgaaa gaaaatcaag aaaagctctt
    16201 tctatttatt gatactggaa actatcgtcc agtcggtgaa aatgtaaatt ggcattctgc
    16261 taaggttcgt tttattttcg ccacgacgga aaaaggtgaa aattatttat tagacacctt
    16321 tgatcggcgt attcaaatta gtgttatgtt gccaactttt gaagatcgcc ccattcgaga
    16381 acgattagag ctgattcaat tattttttca aaatgaagcg gatgttttac aaaaagatat
    16441 tattgtttca agagaagcct tgtctttaat gttatctcat ccctttagtg ggaatattgg
    16501 taagctgaag aatatcatta aaatcagttg tgcggatgtg tatagtcgaa cacaagaaga
    16561 accattaacg attgggaaaa atgagatctt gattcagtta aatatgctgg aatccgaagt
    16621 agaaagcttg ccgattgcta gtttattgat aaagaaaaat caaaagataa aaacggatcg
    16681 aatcaaaccg tttgattatg attcgatttt ggaaaaaatt actcagctga taaatgaagg
    16741 taatttcaat gaaaattttt cttcgataaa gaatgaagta cagcatttgg tgcatcacat
    16801 tgataaggat tcttttttat caatgagtag aaaattgatg attcagatgt ttagaaaggt
    16861 ctgggtatcc gttttaggaa aaacgtacgg attgacgacg agtggacctg ttgcagaaat
    16921 tattagcaga tcctatgtca tttccccaag ctataagaca gacttaaccg ggataaatcg
    16981 tacattagct gcaaatctac ctcggacgac ttacttatgt aatcagttct tggagcattt
    17041 accaccgctc tcagaaaagg acgagaactt tctaagactg cttattgtcg tgactttatc
    17101 tgattatgta gatgaatcca tcgagctgaa gggcctgttg cttgctcacg gagatagtac
    17161 ggctagcagc attcaagcag tagtgaatca attatgcggc aattatgttt ttgaagcgtt
    17221 ggatatgcca gtagagagta atgtgtcaga gattgtcgaa tcgacaagga atttcgtgaa
    17281 acggcaagtg aatacagata gtctagtgct gatcgtagat atgggctctt taaatcaaat
    17341 ttacacacag atcaagaacg atttaaaagg ggagttactt cttctgaata atttgacgac
    17401 ttccattgca cttgatattg gcttgaagat gacgatgaat gcgccattta aatagattgc
    17461 ggaggatgca gcagacaagt attcaatcaa tgttcaatac tttgaaggct tttctcaaag
    17521 tagcaacatt atcatttcct gtatgtcggg tttggggatt tcggacaaat tgaaagatgt
    17581 ttttcgtcat catttatcgg atgaatccat cgaagttttc acgaaggact acagagaact
    17641 acgtgaatta atcgaccaaa atgatgaaac ttattttgac aagacaaaat tggtcatcac
    17701 gacttcggat cttccacgtt ccttttcaat tcctaatatt aatgtttatg acatcttaga
    17761 cggtgatgga gcagccaatt tggagcatat tttgagtcct gaaatgaagc cggcagaatt
    17821 taaccgcttg attcaggagc tagtaaaatt cttttcgttg gaaggaattg cggaccgttt
    17881 gaactttctg aacccaacga tcgtgatgag tgaagtggaa acagtcattt ctaaatatga
    17941 aaattactat catttcactt taaatggccg tgtaaaacta aatctctata tgcatacggc
    18001 attgatgatg gaacgtttat tcttggctcg gacagaaaaa tctgaagaac cgaattatga
    18061 tttgaaacca gatgaagatg aattttatta tgtttctaga agtatttttc aatcaatgga
    18121 gatgaaatat aattttaaag tcaatgctta tgagctttcg ctgctctatg aattattagg
    18181 accgtatatt caaaaataaa gtgtctaaaa aagtgtctaa tctgtttttt ttaggttaga
    18241 cacttttttt aatgtgaatt aacgtgatta aaccaatgtt tttaaaagtt ggcacaggaa
    18301 ttgcttatat aaaagtgaac agaaagaaag gaattaacca tatgaagaaa attctaatag
    18361 cgactcacgg tcacttagct tcaggctttc ttagcagtat tcaattactg acggggaagg
    18421 ttcaagagat tacggtgatt aatgcttatg tggatgagtg tgattttgaa caggagttga
    18481 atgtatttgt aaaccaggtg aacgagcagg atcagatttt tgtttttacc gatttatttg
    18541 gcggaagtgt caatcagaag attaccaaaa cctttttgga gaaagagatt gctgtaacta
    18601 ttttgtctgg ctttaatctg ccaattctgc tcgaaatagt attggcaacc aaggaattaa
    18661 caaaggagga tattcaggaa ctggtaaata aatgccaaca ggaattaaag atgaccattc
    18721 ttgatgaggt acatgtagta aatgaagagg aagatttttt ttaggaggaa gaaaaatgat
    18781 taaagcagtg cgaattgatg aacgattaat tcatggacaa gtagcgatga cttggacaaa
    18841 gagtctaaat ttaaccgggt tagtagtagc aagcgatgag gcggctacca acgatattca
    18901 aaagatgaca ttgaagatgg ctgcaccatc gaatgtcaaa gtcattatta aaacagttga
    18961 tgacgcgatt catttgttaa aagatccgcg ggcgaaggac atgcgcttga tggttttagt
    19021 ccctaatgtc aaagatgcgg taaaagttgc gaaagctttc ccaacggaaa tcgaattaat
    19081 gaacgtgggg aatgctggga aaatgacacc gccatcggaa gataagaaaa ccttatcaaa
    19141 ggaagtgatg ttaacgacgt cagaaattca agacttaaaa gaattagttg aactttatcc
    19201 agataccgtt tttcagccta caccggcgat ggaaaaacgt tcagctatct ctattttgag
    19261 tgatttttag aaaaaggaag gagaaaaatt atgtatgatg catttgtagt cgctttggcc
    19321 gtttttattg gagttgctgg acatgaaatg ttcgggatgt ccatgctgag tcgtccgatt
    19381 gttgttgctc cgttagcagg agcgttgatg ggggatttgc agacggggtt gattgtcggg
    19441 gcgtcactag aagccatctt tatgggtgtc gtcaatatcg gaatctctag tacggcggag
    19501 ccttctttgg ctgctgggct ggctacagcg ttcgcaatca aaatgggagg aaacgtggat
    19561 gcgattattc ccattgtctt tccactagca gtattaggat tacagttaat gaatatgatt
    19621 ttttcttttg tatgtggacc gatggctgct aaatttttca aatttgcaaa atctggtgat
    19681 caaaaaggaa tcatcaatct gcacttcttt atgtggtttg tccactatgc gctttatgct
    19741 cttattccat tctttgccgt tttatttgga gcagatgttg tcaaaagcgt cttggataat
    19801 attccagagg tcatcatgaa cggattaaca gtcgctggaa atctgctgcc ggcagttggg
    19861 atggcgatgc tgttacggat gctttggagt aaaaatattg ctgtgtggtt tttctttggg
    19921 gccgttgtta tggcgtatct cgagttacca ttgattgcaa ttgccgcagt tggaacgatt
    19981 attgcaattg ttatagccca aagagatcta caactaaaaa atatatctgt tcatgcagtt
    20041 caaagcgatg ctgctgtaaa tgcaacgctt gaccaagaag aggaggattt ctttcaatga
    20101 aattagctga tggaatttct aaagatgaga aaaaattggt tcggaagatg ttttggcgct
    20161 cgatgactat gtatgcttcg gtgaatccgg tgacgatggg cggcggtggt ttcgcctatt
    20221 ctatgatgcc cttcattaat catttctaca aaagtgaaga agatcgagct gcggcgctgg
    20281 aacgtcatac acagtatttt tcgacgacca ttccttgcgc gtcctttgtc atggggattg
    20341 cggcatcgat ggagaaggaa aattctgaaa aagctggttt tgacgtggaa agtatcaacg
    20401 cgatcaaaac gagtttgatg ggaccattgg ctggaattgg cgattcattg ttctggggag
    20461 tttggcgtgt cgttaccgcg ggactagcga tcaacttagc caatgccgga aatattttgg
    20521 caccaattat ctttttagtt atgttcaata tacctaatta cttgatgcga tactatggcg
    20581 gcgttttagg ctattcatta ggctctaaat atattgaaaa gctgtatgcg aacgggttaa
    20641 ttgaaattct gacaaaagct gctagtatct tgggtctgat catggtaggt gcaatgacga
    20701 tcagtatggt caaattcaat accgttttga gcttttcaat gaagggcgaa gaactaatga
    20761 atgtccaaga aatcctcgat agtattttta aaggaattgt accgttattg gtaacattga
    20821 gctgtttcaa attactagat aaaaaagtcg gcgtcatcaa aattattttt ggaatggtcg
    20881 tcttgggaat tttattgtca ttacttggaa tcgcatagta ggaagctgca gctactaggc
    20941 tgcagttttt ttagcaagga gggaaaagat gactattaat gaagtaattt attcacccaa
    21001 acacccattt tttaatgctg agttaaacga cggaaatgtg actacttcaa acgaatacag
    21061 ctactatcgg aatattcctg atgtaaaggg attgaccttc gatgtaagta atcaaaagac
    21121 attagcaaac cttgatgttt gcgggacgat tcaaacgttg accttttatc gagaaaattt
    21181 actaactgag gaaaaaccag gtgtttgggt caataaacag ctctctcaaa ccaattcgtt
    21241 atccttaaag gttgaagtaa acgggattgt ctaccatttg tcagaatcgg atcatcgtat
    21301 tgaagtcgat gtgattcaag actgtttgcc tagatatatt catcattatt cgacgtttaa
    21361 ggtcattgta gtatcctttg ctcccatact ggctaataag cgtttaagta tgcttgttca
    21421 acaggtttac attgtgaatg aaacacacga aagccttcaa gtttcagtga aagatgtgcc
    21481 ttcgtatcaa gagaagtatt cggatcagca aaatgtgttg atttctcaaa aaggaaataa
    21541 ggacacgatc gcacaacagg aagtggcaag cttcgccatt gcttttgtgg acccaaatgc
    21601 ttttgaagaa gtaatgactt ttcaggaatc tgatatcgaa aaatggctga aggaaacatt
    21661 gaactatttt gctcagctat ttgggcagct gtcaatgcca aacgatagag ttgtccatct
    21721 ctatcgtcgt gcactttatc aaagcttttc tagtttcggt atgaatcgac agggacaggt
    21781 cgtcggcagc aattggggca gctatcctgc tactaatcga atttggaata aggatatgta
    21841 ttacgcatca ttgccattca ccatgtttga accggagtta tgtcaaaaaa cggttctatg
    21901 gtttgatcaa tatggcgtta agtttcctgg ttcaaaattt acaggaggta tcaatcattc
    21961 tctgagtaat tcgttatcca gtgcgttact ggctagtctt tattttgaac atactgggga
    22021 tacaaaattc tttgagcggt gtccgcaggt tctactgaat gcttcaagaa ttattgagga
    22081 catattagct caacgtcggt caggggaacc aatgcttttt ccatctgttt ggttatcaga
    22141 cgcctttgca cttggaaagt atcataccgg cagcaatctt tgtctttgga aggcctgtat
    22201 tggacttgca gaaatttttg aagccctaga acaactgagt cttagcaagc gatacaaaaa
    22261 cattgcctgt aaactaaagg aatcaatttg taaacaaatg actatcgatg gtccttttgg
    22321 tgagcaattt ttggagggaa tcggtgatga ggagaaaaca acctattcag tgaagcacta
    22381 tcaaaaaccg attcttgagc aagggttaat ctttttaagt gatgtgattg tggatggaaa
    22441 aattaattta ttgatgcatg atggagaaga atcagacact acgttgatgc ctttttatca
    22501 atttttagat aataaagatc atttatatca gaacaccatg gccttttctg ctagttcttt
    22561 caatccaacc tatagcgagg aaatcaaagg catcacttgg ggattagaat ccggcgccac
    22621 ttttcctggc tttatcactg ttttaatgag cgagctgcac aaccctcaag ctttcgtaac
    22681 ccgattagaa gaactaattt gtttggcaga tttagacggt tcgtggtggt ggtggccgta
    22741 tcgcttagag gctcaacaag gagaagtcgt gcgagacttt ggctgcggca aatgcggctg
    22801 ggcatcgggt ctttttgtca gcctaatgat cagtcaatat ttcggattga aattcaaaaa
    22861 aggagtattg caaattgatc ctgttgataa tttaacgttt tcttggaaga atttgaagtt
    22921 cggtcaagcc ttcatttcga ttgaatgtac gcaatccgag ttgagtattt caaatttgtc
    22981 agaaaagcca ttgaaaataa gtgatacgaa aaaaattctt cacgtagaaa agggaaaaac
    23041 gattcacatt caaaggagaa aaagatgagt atactaaaaa atattaccca atttaaaaaa
    23101 atccagagtc gagcaatcac tcccgaaaat ccaacaggcg agaaaggtaa agctgcgatg
    23161 ttgccaagcg agctaggacc aagccgcaaa ggtcgtggcg caattccgct gacttctggt
    23221 gaagaaactg tgattgcgga tattactgga acgggaataa ttcgtcatat gtggatgacg
    23281 attcgtgatc gaacagaatt tggcagtttt gttttacgtg atttaatctt aagaatttat
    23341 tgggatgggt cggatagccc tgctgttgaa agtcctttag gggatttctt ttgtaatggg
    23401 tttggagagc gttgcgaggt gaactcgttg ccgatagtgg ttaatcctac agggggcttt
    23461 aattcctatt tcgaaatgcc tttccaaaca tccgctaaaa ttacgatcga gaatttacat
    23521 ccgaaggata tgtcctcatt tttttatacg ataaattacg ctttggtgga cgaattggaa
    23581 gaaaatagcc tatattttca cgcaaattgg catcgggaac gcattaccaa attacaggag
    23641 gattataagt taattgatcg attaaagggt caaggctatt acgtgggtac ttaccttgcg
    23701 cttacctgcc tagaacgtta ttggtggggc gaaggcgagt ttaaattcta cattgatggg
    23761 gatagtgagt atccgaccgt aacctcaaca ggttcagaag attattttgg cggggcgtgg
    23821 gcctttcatg agtacgatga gttcggacgt ccacatgcga aaaactattc aacagccttt
    23881 ttagggtatc cttaccaatc aaatagagat gccacaagag atcgttttga aacgggaaaa
    23941 ttgaatgccg ttcatgcctt tggtgacgac gcaatgccac gacatggtct ttatcgttgg
    24001 catcttcaag accccatctg ttttaccgaa gatttatcgg tcactttaca acaaatcggc
    24061 aacgatgatg ttcgcttatt tgagcggcaa gacgatgttg ctagtgtcgc ttactggtat
    24121 tcaacagaag cttcaggaaa tactaaacgt catttcactg atcgagaacg cttgccgcgt
    24181 taatgaagtg tttttcacat attcgaaggg aggaacctat gatgatatat ggggcaattg
    24241 aagctggagg gactaaattt gtctgtgcag tcggtactga ggaattaacg attttggaaa
    24301 gagtcagttt tcccacaaca actccagttg aaacaatgaa gcaggtcatt gattttttta
    24361 aaccttttaa ggaacaattg gcaggcatcg gggtcggctg ctttggtcca attgatattc
    24421 aatgcaaatc aacgagtttc ggtcatatca catcaacacc aaaacttgct tggcaaaatt
    24481 tcaattttgt aggaacgcta aagcaatatt tcaatattcc gattgaatgg acaacagatg
    24541 tcaatgcggc ttgctatggt gaatatgttg ctggttctgg caaagggtta gcaggtgttg
    24601 catacttcac tgtgggaaca ggtatcggag gtggtgcctt gttcaatggg actttcgtgg
    24661 aagggttcag tcatccggaa atggggcata tgttgatcaa gcgacatcca aaggacaatt
    24721 tttcaggaaa ctgtcccttc caccaagatt gtttagaggg gatggcagca ggaccagcga
    24781 ttgaaaaacg gttgggtgta aaaggccaga atttattggc tgacgattcg ttttggcaga
    24841 ttgaagcctt ttaccttgcc caatgtgcat acaatacaac actgatgttt tcgccagata
    24901 gaattatttt tggcggtggt gtaatgaaac aagaacacat gaagaaaaaa gttcaggata
    24961 aatttgttga gcttataaat ggatatgttg aaataccacc aattgatagc tacattatta
    25021 cgcccgaatt aggtgataat gccggaataa tcggcggatt agcattggcc agaaaagcgg
    25081 taagaaataa gcaaccataa ttaatgaatg tttcattgat actcatgtca aaagtattta
    25141 accgagtaat caaatgatca aatgaagttg gataaagcga aaatgaattc aaataaaaag
    25201 gctctaagtc aaatcgtgtt gttttcatta aatcactgaa aggaaatgct tctgcgtttc
    25261 cttttttctt tttgttgtgc gctgtatacc ttgaggatat tcactggtgg cgtacactta
    25321 aatagtaata tattgtgtta ctttatgtct gtggtaatag ttatagttgc ttaccaatgt
    25381 atgttcattt ttcgaaatgt tgacttcata atccttggct taatgtttga atttttttgg
    25441 tgatagattt tttcagccgt ataaaataaa tcgtaattct attatagaga gttgtgatta
    25501 taaaaagata gcaagaaaaa aaaagaaagt ttctatacat tttaaatatt ttattcctat
    25561 ttctattgct aaaaaagtag aaattctata tttaccttta actctcgctt ctcttgcaaa
    25621 tgtagttagc attttaatcg at
//
LOCUS       DAZBSA010000035        25411 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000035 DAZBSA010000000
VERSION     DAZBSA010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.035"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1835)
                     /locus_tag="ACHGMT_001299"
     CDS             complement(<1..1835)
                     /locus_tag="ACHGMT_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFN6835049.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="ACHGMT_001300"
     CDS             complement(1832..2536)
                     /locus_tag="ACHGMT_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFN6835050.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="ACHGMT_001301"
     CDS             complement(2548..4857)
                     /locus_tag="ACHGMT_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFN6835051.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="ACHGMT_001302"
     CDS             complement(5052..5516)
                     /locus_tag="ACHGMT_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835052.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="ACHGMT_001303"
     CDS             complement(5516..6142)
                     /locus_tag="ACHGMT_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFN6835053.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="ACHGMT_001304"
     CDS             complement(6149..6514)
                     /locus_tag="ACHGMT_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFN6835054.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="ACHGMT_001305"
     CDS             complement(6504..6842)
                     /locus_tag="ACHGMT_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835055.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="ACHGMT_001306"
     CDS             complement(6832..7158)
                     /locus_tag="ACHGMT_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFN6835056.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7139..7417)
                     /locus_tag="ACHGMT_001307"
     CDS             complement(7139..7417)
                     /locus_tag="ACHGMT_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFN6835057.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            complement(7419..8783)
                     /locus_tag="ACHGMT_001308"
     CDS             complement(7419..8783)
                     /locus_tag="ACHGMT_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFN6835058.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="ACHGMT_001309"
     CDS             complement(8796..9365)
                     /locus_tag="ACHGMT_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFN6835059.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="ACHGMT_001310"
     CDS             complement(9337..10524)
                     /locus_tag="ACHGMT_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFN6835060.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="ACHGMT_001311"
     CDS             complement(10588..12315)
                     /locus_tag="ACHGMT_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFN6835061.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="ACHGMT_001312"
     CDS             complement(12312..12764)
                     /locus_tag="ACHGMT_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFN6835062.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="ACHGMT_001313"
     CDS             complement(12876..13256)
                     /locus_tag="ACHGMT_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFN6835063.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="ACHGMT_001314"
     CDS             complement(13253..13639)
                     /locus_tag="ACHGMT_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFN6835064.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="ACHGMT_001315"
     CDS             complement(13641..13919)
                     /locus_tag="ACHGMT_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835065.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13967..14695
                     /locus_tag="ACHGMT_001316"
     CDS             13967..14695
                     /locus_tag="ACHGMT_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFN6835066.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(14826..15302)
                     /locus_tag="ACHGMT_001317"
     CDS             complement(14826..15302)
                     /locus_tag="ACHGMT_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFN6835067.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(15262..15618)
                     /locus_tag="ACHGMT_001318"
     CDS             complement(15262..15618)
                     /locus_tag="ACHGMT_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835068.1"
                     /translation="MKKVNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVL
                     KIEQNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIR
                     GGVCEISLVKRLPRTR"
     gene            complement(15596..15781)
                     /locus_tag="ACHGMT_001319"
     CDS             complement(15596..15781)
                     /locus_tag="ACHGMT_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835069.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(15782..16054)
                     /locus_tag="ACHGMT_001320"
     CDS             complement(15782..16054)
                     /locus_tag="ACHGMT_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFN6835070.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(16051..16251)
                     /locus_tag="ACHGMT_001321"
     CDS             complement(16051..16251)
                     /locus_tag="ACHGMT_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835071.1"
                     /translation="MKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
     gene            16379..16591
                     /locus_tag="ACHGMT_001322"
     CDS             16379..16591
                     /locus_tag="ACHGMT_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835072.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(16624..16818)
                     /locus_tag="ACHGMT_001323"
     CDS             complement(16624..16818)
                     /locus_tag="ACHGMT_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835073.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(16831..17013)
                     /locus_tag="ACHGMT_001324"
     CDS             complement(16831..17013)
                     /locus_tag="ACHGMT_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835074.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            complement(17010..17150)
                     /locus_tag="ACHGMT_001325"
     CDS             complement(17010..17150)
                     /locus_tag="ACHGMT_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835075.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            complement(17640..18005)
                     /locus_tag="ACHGMT_001326"
     CDS             complement(17640..18005)
                     /locus_tag="ACHGMT_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835076.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(18045..18347)
                     /locus_tag="ACHGMT_001327"
     CDS             complement(18045..18347)
                     /locus_tag="ACHGMT_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFN6835077.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(18347..18646)
                     /locus_tag="ACHGMT_001328"
     CDS             complement(18347..18646)
                     /locus_tag="ACHGMT_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835078.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(18639..18803)
                     /locus_tag="ACHGMT_001329"
     CDS             complement(18639..18803)
                     /locus_tag="ACHGMT_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835079.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(18809..19078)
                     /locus_tag="ACHGMT_001330"
     CDS             complement(18809..19078)
                     /locus_tag="ACHGMT_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835080.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(19090..19839)
                     /locus_tag="ACHGMT_001331"
     CDS             complement(19090..19839)
                     /locus_tag="ACHGMT_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6835081.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(19836..20528)
                     /locus_tag="ACHGMT_001332"
     CDS             complement(19836..20528)
                     /locus_tag="ACHGMT_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFN6835082.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            complement(20534..21205)
                     /locus_tag="ACHGMT_001333"
     CDS             complement(20534..21205)
                     /locus_tag="ACHGMT_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFN6835083.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(21198..21539)
                     /locus_tag="ACHGMT_001334"
     CDS             complement(21198..21539)
                     /locus_tag="ACHGMT_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835084.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(21717..22415)
                     /locus_tag="ACHGMT_001335"
     CDS             complement(21717..22415)
                     /locus_tag="ACHGMT_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFN6835085.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            22502..22972
                     /locus_tag="ACHGMT_001336"
     CDS             22502..22972
                     /locus_tag="ACHGMT_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835086.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(23015..23194)
                     /locus_tag="ACHGMT_001337"
     CDS             complement(23015..23194)
                     /locus_tag="ACHGMT_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835087.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(23181..23519)
                     /locus_tag="ACHGMT_001338"
     CDS             complement(23181..23519)
                     /locus_tag="ACHGMT_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFN6835088.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(23535..23738)
                     /locus_tag="ACHGMT_001339"
     CDS             complement(23535..23738)
                     /locus_tag="ACHGMT_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6835089.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(23753..24529)
                     /locus_tag="ACHGMT_001340"
     CDS             complement(23753..24529)
                     /locus_tag="ACHGMT_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFN6835090.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            24828..25148
                     /locus_tag="ACHGMT_001341"
     CDS             24828..25148
                     /locus_tag="ACHGMT_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN6835091.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            25166..>25411
                     /locus_tag="ACHGMT_001342"
     CDS             25166..>25411
                     /locus_tag="ACHGMT_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFN6835092.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEA"
BASE COUNT         7100 a         5266 c         3943 g         9102 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 agtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggata
    14221 ttctgaaaat attttaatta aagattatta ccgtccgccg attaaaatag atttaccaga
    14281 aaatattgaa aaagtttctc ctacatttgt tgatatttat actcaagcca cacaagctaa
    14341 atctgagcaa ttggaacaaa ttgctggagt tggttataga aaagctctag aatttctaat
    14401 taaagattat gcaatatcta aaaattcaga ggatgaagat aaaataaaaa aaatgttatt
    14461 aggtaaaaca attgaaaatt atttgaatga ttttcctaaa ctccaaagat tagccaaagc
    14521 tgctacttgg attggtaatg atgaaacgca ctatgttcgt agacatacta ataaagatgt
    14581 tcatgatatg gatgcattta ttaaatcagc agcgcaattt atcgcggctg attatgatgc
    14641 tgatttagct gatcaattta taaatggaaa taactttatg ccaactacta attaaaagaa
    14701 taaaagagcc acagatacca attaataaaa cggttgggat atcttggctc tttcatattt
    14761 ttttgacact aatagaatat catgctgcta acaatgttta cgaacattta gtgaacagtg
    14821 taattttaga ataaagagag ttgttctttg tacttctcgc tattgtaaaa atcaatttcg
    14881 ttttcttttt tcaatcggtc agcttgtctg tgttcatact catctaaaaa gttcaatgtc
    14941 tttctaattt ctgcatgtcg ctgtctgaca tacgatgagc tatacccaac agattcagca
    15001 atatcttcta agctcatttg atcaacatat ttcatcttaa caatttgatt atcgatacca
    15061 gaaaagctat ctatcagcaa taacatttct tctttttgct cgatcaacaa ttccaattca
    15121 ttttctatct tttggatatt ctcttctaaa gaagaagttc tcgaattctt ttcgatgcgg
    15181 acgtttgcta aatcaccaca gacccatcga ttcaattcaa gcttactttt attaagattc
    15241 cactttaagt aaagaatctg ctcatctagt tcttggtaat cttttaacca atgaaatctc
    15301 acaaacgcca ccccttatgg tagaatagta ttgcgctgct atctcgaaag gggtagcttt
    15361 tttatttttt cttagccgtt ccattctgga ttgccttttg ttccaaacgt cgttttttct
    15421 tcttaatctt tgattttgtt tttcccatac aattctcctt tccgaaaaca cattttgttc
    15481 gatttttaat actaaataaa agttggccag aatttttcgt tcgtctttac tcatcttttt
    15541 ttgaataaga ttactagcat aaaaatagtt agtaccacga aaatgaacag gtaaatcaat
    15601 tacgttcacc ttcttcatgc cagttatcag ccactagaat ctttgcgtta gtgccgccac
    15661 cgttttcttt tttgtatttt tcaaatgctt tataagcagc tgtttttgat ttatagattt
    15721 ttccggaacc gactgggaac atccatgtgc cctctgtgat agctaagatg tatactttca
    15781 tttaattcac ccctttaatc cccaacacta catatccgga cttctgcatg taatcagtaa
    15841 tataagtgat ctctgctttg tatgagtcac cagtgtaact aaatgttggt ctcgttagtc
    15901 caggtaattc atgtttaatg tattttttat attcacgtag tattaactga tcaccaactt
    15961 tgaagtcacg atcattcttg cggatctcga attgtttgcg tccgctcgtc actgcttcaa
    16021 aatattctgg tagtattttt agttcgtgga tcattctgtt tcctccttca aaattcgctc
    16081 atcatcaaaa agtgaatctc cacaaaatcc ttctcccaaa tatttatcgt tcatgtataa
    16141 gtcaaccaaa tatttgtgat ctgcttctcg atattcatta atattaacga tctttatttt
    16201 gtaaacatta ccttcgtcgc tcactatctt gaattgttct ctgactttca ccaccgttcc
    16261 tcctcttttt gaaaaaattt tagtgttttt gtaagacccc ctaaatcagc ttttttattt
    16321 gatataatac ttgtatcgat taatcaaatc gaaattaaat ttaaaaaggt gatttatcat
    16381 gaatatcaac aatttagtaa aactcgttga acaacgtttt aaagaatcag gattagaaca
    16441 attaaaaaaa gataattctc cagaattatt ttctaatttt accgaagacg acattcctac
    16501 tcttaaacaa ttaaaatatt attctgatta ttggacgcaa actcgtgaga atgaattaaa
    16561 tttagaaaaa ctcattatta aaaagaaata gcaactccag cccacacggc tggtttttta
    16621 gtttcaactt ccctccttac gggtcaaaat catcggttcc ggcataatct gatcatcctt
    16681 tagcaacaaa tcaggatatt ttttccttag cttcttattg ttcgtgtaag ttttcaatag
    16741 ccattggttg gcttcgcttt tgctttctcc agaaaaataa attttccctg tttggatgcc
    16801 tgcaagttga atcatcattt tcacctcaaa tcaaattttt tctgctagat ggagcaaaag
    16861 caatccgaca tcaagaatct tttgacccat tatttcagct actttgctcg gcttaactcc
    16921 ttgagaaaag agcaagcgtg cttgctctat ctcttcgtga gtccaaataa atttggcttc
    16981 ctcaaggata attactggat cagttgctct cattgttcct ctggaaaaat gatgtcattc
    17041 ttatgcttgg accaggtatc tgcatatgga acaaagtatt gtctagctaa ttcgatttga
    17101 tcaattaaag cagtttctga taattcatgg tctgcagcaa tttcattcat actatcgcct
    17161 tcatctaaac agataagacc gttgacgtta tcgattttta catttgtgac tgcttgactt
    17221 tctccacgct cataatattc tacagttgat actgatacat cagcttcgga gtttaattgc
    17281 agaatctgtt actgccggga taatttaata ttcttttcat aattcacctt ttttcctact
    17341 gacatcgaat atttttcatg ttatgattag ccgtgaaagg agagctatat tatgtgttta
    17401 cacatgatgt ccttagctca ttcggttctt tggaagcttt tttggaaata actctcaggt
    17461 tcttgacgca aatctcattt attctttgaa acaaaaaact tccattttcc gagagctttg
    17521 gatcacttca aaagagattt gcatttatat ttctgtgacc ttgagtcgcg taaagaacct
    17581 tacagcaatg gcggcaattc cactttcagg aaaccgcctt ttttcattga tattggtggt
    17641 tagcggacta cccattttct ttcggcagtg ccaaaccaca aatgatctgt gttgatgttt
    17701 aggtagttgt ataccagata agctaaatcc aaagtgcctg catcttcaag acgacaatat
    17761 tccaaaaatt ctcccatagc tggacataaa aatcctggag ctatcatatc ccaacagtta
    17821 atagtgcaaa gaaattcgtt tagcttctga acgttttctt tggtttcacg aattacaaca
    17881 tactccaatt cttctccgct ggcagagcat tccaaaactt caaattgatt aagaaatggt
    17941 agaacatatg cttcaaacat caacattcca tctttctgtt tttgcacttg ttttgcatca
    18001 atcatgtcta tcctcctatt cagttatttc ttccattaat ggaattattt caggtcactg
    18061 gacttaacga aaacaccatt gaccatcttt ccagtccgac ctttgatttc gtcataagca
    18121 aaatccaagc actcgtataa atccatatca ttttgcattg ccaagatgat caaggttact
    18181 actacatcgc caattccgtc tcttaaacca tgttcatctt ttctagctag agaagcggca
    18241 acttccccga tttcttcgac cgtttttaac atttgcttgc tgaaatccgc ttgatccaat
    18301 cccttatctt ttgcccactg ctctactttc gcgattagtt cgtccattat ttctcctcca
    18361 catacctaaa ctgtcttcct tttgaatcaa tccataagct cctagctcta tcccaaataa
    18421 tatttttgct cagaccagta atttcagata actgtttagc ggtacccgtt actagaattc
    18481 gatcaccatg ccagattgca atctttctcg gcgttctccg tttgggcttt tcagtccaca
    18541 ttgatttacc gagcttttgg acttctgcaa ctattttttt atcttcctgc caatcttctg
    18601 aataagtcaa ttcgataatt cgctgcattg ccgctttctt atccatcccg acattctcct
    18661 ttcagtaatt tgagtacttg atcaagtgcg ctctcacgtc cgccatgaaa tgtgttgagc
    18721 cacttgtctt cgtacgacac actttgtctt aaagcttctt gatgcattaa ttcgatctgt
    18781 gctgtaaatg tctttaggtc catcttgttt acacctgctc aagttcatta agatgttttt
    18841 gcaagccttt aacacaatca atgaatagta atttggtata tgctaaattt cttaattgcg
    18901 ttccatcgat atagagtgcg aaatagtatc tgagtttact ccaactcgaa cgatcattct
    18961 taattccttc aattccagct tcttcgagtt gatcatatac gtctctcagg acgtctaatt
    19021 cctcgccctt tttataattc gctatttcat taattagttc tagataatcg atcttcattt
    19081 ttccacctct tagaatggtg cttttgattg tctattagct cgttctagcg cttttttctt
    19141 gagataggct tcttggtcga ttgcccattc aggaagcttc tctcgtcttc ctgtgcgctt
    19201 gtatccactg cttgcgttct taggttcact tttttctttc cttgcccaac ttcgaatagt
    19261 tgccaaatag tttttataag tcttaccaga tgattcacaa tactctgaca gtcgttcgat
    19321 tcgctcttgg taatcattag ggaattctgt tttgagtttc tccatctgct catctgacaa
    19381 aagaacattt ttatactctc cgtatttatg acggacgggc ttagccttcg attttttcga
    19441 aggcggtaac tctcttatat attcttttgt attattaaat gtattattaa tagatgtatt
    19501 attatctttg actttttcgt caataggggt attgcgtttt tcgtcaatag ggtattgatt
    19561 aattcgtagg tacctattga ttatttgatt ggtaccctct ttgtaaatga tttcccgatt
    19621 caagtatcca aacttaatca aatcacttac ccatcgcgat atggtctctt tattcacacc
    19681 atataaatct gcaaagtact cattgcctgc ccaacaaaag cctctttcat tacacaaggc
    19741 cgttatctct ccgtataaca acttagtatt tggtttaagt cttttgtcgt accttacgtt
    19801 ggctggtata atcgcataat aacttcgatg ttctgtcatt tttcccctcc aatatttaac
    19861 tttttgattg ttttctggtt taatttgatc cctttgattt gatatttatt tttgaaatta
    19921 atcacaccta ttttgtgctt ctctgtgtga tggattctgc agagtgctgc aaatgtgtac
    19981 tctgcatgat caacttcttt gcgctttcgt cttcctagcg ctttgtcaaa gtgatcgatg
    20041 tcagctcctg ttttgccaca gatgcagcag actctttttg tgatgcattt gtagaagtaa
    20101 tattcttgat tcgcaggtaa aatctcatag ccttctttga aaggaatatg atgttcaaag
    20161 atgaaatcta agatgatatt tgctaagaca ttggcatcac tcacagttgt attcgattcg
    20221 tctttcaggc ttattttgcg ccctgtgaca ccttcaaaac gaaagtagaa gaattccttc
    20281 cagaagtccg ttggcatgcc tgtatcgata aaaatatcgc ctatcagcgc atagatgaag
    20341 tttcgttgct gtacggtgaa acgtctagga tcaataaaac gaatttcaat gactcgatca
    20401 ccatcgtagc cgtcatacat cgtctttagt cgatcaatgt tcacttcctc attgatggtt
    20461 gcgcttatgt cttttccttt gaactttttc agaaccgctg aatatgaatc gattaatggt
    20521 ttaaacactc atatcacttc ttatctaatt cttttctctt agctgctatt gctcgctcca
    20581 tcaaggcaca ttgctcatag cttaactgtt caatagtttc aacgttatca gctaagagcc
    20641 ctaatttatc tgtctgctca ttaacatatt cgattaaggt tttggtcata tctttaccca
    20701 tctgctcatt gaaagcttct agaatcgtct ctagcatgtt taatttcttt gtatcgattc
    20761 taggtggtgt tggaatatct tccccttgaa atacatataa tcccagtccg tgtagagcca
    20821 atgctttcac aaagcatcgc ttcaatgagt tattgatttg cattgcattt ggtttaacaa
    20881 ctggttggtt tcgataatct aaaacaggaa ataattcggt ttccgtgtgt cctttaaccg
    20941 ttactgagac agatacataa gtcccagttt catccataag aaaaggttta tattcctcaa
    21001 caagaaagtc ttgatgagtt ccagaaacaa ccctgtagtg tttgtactca ttaatagtta
    21061 ccgttgcctg tggatcattc tttttcataa tctcccacgc gtgagcccaa gataaataat
    21121 caaaatttcc ttttttcttg agaattttat ttaacttacg actaaaaagt ttttcaaagt
    21181 tcgttgtccc tttgatttca ctcatcaaat tctgcctcca tttcagcaat gtatttctta
    21241 cctggtccgt aataagagat atcaatcaag ttatctctgt cgtactcttc tagcgcatca
    21301 atcaagccat cttcgatgac gtaaatatat tcaggtttat tcgaatgctt cgatagatgg
    21361 ataagataaa catgatccca aatagttaca tggttgccta aatcatcttg atcccaagct
    21421 agttcttcat tcgtcaaaag atttcgtctg atttttcgac cacttgtttc ctcaattttc
    21481 ggcttgcccc aatcaggatc aatcaaatat tgatctagag tggaaagttc tttttccata
    21541 tgctaaaatc tccttatgat gtgttttctt tgtgactctt tgcttgccgg cggagtcact
    21601 tttttatttg ttgccatgct ttttgcttgt caatatgttg ttggcttagg atgattggtt
    21661 tatagtattt ccaccagcaa ttagcaattg ccgtccctat tcttagcgct tcagctctat
    21721 tcattgtcat caccaaaaag tctctgttgt ctgttcagtt gatcaatttc catacggatc
    21781 gcagtttctg gcaaccacat ttcaataaat gaaacagcat catcgaatct cttacgaggt
    21841 aactcgccat atcttgggat tgaaaaggta cgtttaaatt cagaccaaaa ttttgagaat
    21901 acttttttgc tgatttcttc ataagctcgg ctttcttttc cccctagaac tcccataact
    21961 ttcatatttc ctttttgctt aatttcaaac tcttgttgtc cgctaattcg catagtatct
    22021 ttaagcatgg aaacatcttt tttaacatct ttcatttctt ctaattgata gatcatcata
    22081 tcttcaattg tttgaggaac agtattcttg cgaataacat cttccatttc gttgaatgct
    22141 tcaatgtatt tttgtttgaa gtagatagct ttctttcctg taaaccccat agccagcaag
    22201 aaaaatccat ctctactaat gaagaaaact cgtcgatttc tgccatatga atctggttca
    22261 ttaccttcta caaacatctg tccaaaattg gacacatctt tttttagtgc atcaatatct
    22321 cttaaaacat gttgatgttt tttctcgaag ctttctgcca cttgtaagct cgtagtcaca
    22381 gcttctttat ttttcaaaat tactaattct tgcattattt cttctctcct ttttggtata
    22441 atttagttaa aaactggtgg tgtttatttt gaattttttt cacttaacaa tttttcaatg
    22501 gatgtcaata ataagtttta tattgagtac gtttttaaca gggataaaaa taaaaggaat
    22561 gcgccctcaa ctagaagttg aacttaatgc ttcttacttc gctgcagata tgatttatac
    22621 aaaagtaatc atttctaact attcaacaga accagctatg ttagttaatt tagaactttc
    22681 atccgctaac ttaaaccgca gatggtcagc tactccattt aagaaattaa ttgctaaagg
    22741 aggatcagtc gatgataata gaatatactc tgaagctgta ccgctaaata ttcctcctaa
    22801 aagtgctata tcttgttacc ttgcatttga agtagggaaa attaatttta ataaactcct
    22861 taatcaccaa acaaaaatga tatttactct aaatagaacc caaatccaca aaattgttga
    22921 tatcaaaaat acaaacttcc caatagagaa attagtgaaa gaattaaact gagtaatagt
    22981 aatatacatt ctagtttctc ttttctattc atttttagtc agccccctcg gttggctttt
    23041 tcgctctgta ctcagcttca gcttcatcaa gccccataaa aatccaaacc atgtaaacaa
    23101 tcgttcctat caacgcttgc ctgcttcccc aaagtcctaa agcgtagatg attagcggtg
    23161 cgctgaacac tagcgctcta ttaaattttc ccatccgctt gcctccttaa gatttccgaa
    23221 aaatttgttt ctaaaaattc aagtgtttta cttcttaaaa atagatatgt gtctcttcct
    23281 tcaactggat aatagacaaa tccatttttg tttttctcga tatcgataat atttctatat
    23341 cttgggttct ttaaaactct agaagtaaac caatcatatt ttctgttaat ccgttctagc
    23401 acttctggca acgtcatcca tctaccagta tcatcagctt tttttaactc ctcataatcc
    23461 acttgggaga taattacata gccttctgga attgggattt ttgcttctag atattgcatt
    23521 agctgttcct ccctttacaa ttcgtacata gtgataatcg aatctataat tctgtttgct
    23581 tctgcagaag tctttttacc gtttaaaatt aaagataagt agcttttgct aattccaaat
    23641 ctttcagcaa gcatggtgta agttaagaac tttgaacttt cgacatattc tttgattttt
    23701 tctctatctc gttgagtgat ttctgcaatg tcagtcatac taaaactcct ttctaaccaa
    23761 tttcctctaa atccatttga gggtaatatc cttctttttt tagtaattcg tagataaata
    23821 gacgcccttt ctgtgtccat ttggtattca ttacaatttt agtgccacca tcggctttcg
    23881 ggatctcagt tgtatgagat tttgtgtatc cttggttcat gtgttttttg cacaataacc
    23941 attggttacc gacttttttc tgaataccta gtttatgaag taatttattc atctgttgtg
    24001 gagacatccc ataatctgct gcaatctgac taattgttac tgaatctgta gaagataata
    24061 tgctatctaa ataggagatt ttaggttcgt actctgcaat cttttgttct gcgattaatc
    24121 ttccagttct ttcttctttt agttgagttg ctaattgaat gattgtatct ggattaagca
    24181 aagcttcttc tactttttct ggagttagat aacctccatg ttttctaatt gctggcaaca
    24241 cttcacttgt tacccaacgt ttaaattttt tggcagaggg aagttttgat tttaagatta
    24301 aactgtacaa accagattcg ttgatgattg tcatctctct tgattgacct gaggtcgtga
    24361 ttcgcgaccc catcttatct tccaaatcta catgccgcga caaagcatct ttagtgtttg
    24421 aataacccaa aacactcgca acgtctttgc ctacaaaata tggttcatca tttactaaaa
    24481 ttgtccgaac ttcgttttgt tcgaaattaa aaatttgcgg tgtgttcata tttctcattc
    24541 ctttctttta tattcgtaaa caaatttaac aacttttttc taaattcaat tgacagtatc
    24601 tgagtaatac tctatactaa gagcataatt aaataagaca taaaaacatt gattttaaag
    24661 ctttcttggc ggtctgcatt tacatatcaa tagtgttttt tgttgtcttt ttagttgtta
    24721 aatctgttta caagatatag tatagctcat tactcagaat tagtcaaatt atttctgcgt
    24781 atttttctaa actttttttg taaacattta gaaaggttgt tttatcgatg aatacctatg
    24841 aaataataaa agacttggcg aagaggaaaa agatatctat acggcagtta gaaattaatt
    24901 ttggatattc taacggttat ctagcaaagt ggaaaaataa tactcctaat tcagatgaat
    24961 taagtcggat tgctgactat ttcgatgtct ctgtagacta ccttctaggt agagaagaaa
    25021 gagaaacccc taaacatgtg gatttatcag aagacgatac tgttttttct tttgatggaa
    25081 aagaaatatc taaggagaca atgcgtaaag cgattgcaat tgctaaagct ttagaggaaa
    25141 atgaatagtt ggagtgatgg tttgtatgta tttgaaattg aaagaaatgt tgagcgaata
    25201 caatttgaaa gtagtttata tggagatgaa agagcctggt ttctactatc caaaacccag
    25261 aatcattttt ttgaatgaaa atctatacgg tgaaactgca gaagcctttc atttatctca
    25321 tgaacttgca catttcagcg cctctcattt tgaattttca gttttgtacg atacttctac
    25381 aacttttcac tcaaaatttg aaacagaagc a
//
LOCUS       DAZBSA010000036        25081 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000036 DAZBSA010000000
VERSION     DAZBSA010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.036"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            214..498
                     /locus_tag="ACHGMT_001343"
     CDS             214..498
                     /locus_tag="ACHGMT_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFN6835093.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(815..1198)
                     /locus_tag="ACHGMT_001344"
     CDS             complement(815..1198)
                     /locus_tag="ACHGMT_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFN6835094.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(1200..1784)
                     /locus_tag="ACHGMT_001345"
     CDS             complement(1200..1784)
                     /locus_tag="ACHGMT_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFN6835095.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="ACHGMT_001346"
     CDS             complement(1831..3306)
                     /locus_tag="ACHGMT_001346"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFN6835096.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="ACHGMT_001347"
     CDS             3487..3798
                     /locus_tag="ACHGMT_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835097.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="ACHGMT_001348"
     CDS             complement(3913..6249)
                     /locus_tag="ACHGMT_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFN6835098.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="ACHGMT_001349"
     CDS             6391..7059
                     /locus_tag="ACHGMT_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN6835099.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7062..8105
                     /locus_tag="ACHGMT_001350"
     CDS             7062..8105
                     /locus_tag="ACHGMT_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN6835100.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8342..9223)
                     /locus_tag="ACHGMT_001351"
     CDS             complement(8342..9223)
                     /locus_tag="ACHGMT_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN6835101.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9382..10110
                     /locus_tag="ACHGMT_001352"
     CDS             9382..10110
                     /locus_tag="ACHGMT_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFN6835102.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10266..10583
                     /locus_tag="ACHGMT_001353"
     CDS             10266..10583
                     /locus_tag="ACHGMT_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFN6835103.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10576..11184
                     /locus_tag="ACHGMT_001354"
     CDS             10576..11184
                     /locus_tag="ACHGMT_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFN6835104.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11239..12030
                     /locus_tag="ACHGMT_001355"
     CDS             11239..12030
                     /locus_tag="ACHGMT_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFN6835105.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            12168..12656
                     /locus_tag="ACHGMT_001356"
     CDS             12168..12656
                     /locus_tag="ACHGMT_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFN6835106.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12707..13579
                     /locus_tag="ACHGMT_001357"
     CDS             12707..13579
                     /locus_tag="ACHGMT_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFN6835107.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13755..14117
                     /locus_tag="ACHGMT_001358"
     CDS             13755..14117
                     /locus_tag="ACHGMT_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFN6835108.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14126..14878
                     /locus_tag="ACHGMT_001359"
     CDS             14126..14878
                     /locus_tag="ACHGMT_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6835109.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14892..16694
                     /locus_tag="ACHGMT_001360"
     CDS             14892..16694
                     /locus_tag="ACHGMT_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6835110.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16971..18011
                     /locus_tag="ACHGMT_001361"
     CDS             16971..18011
                     /locus_tag="ACHGMT_001361"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFN6835111.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(18063..20207)
                     /locus_tag="ACHGMT_001362"
     CDS             complement(18063..20207)
                     /locus_tag="ACHGMT_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFN6835112.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLRLDKSFWLDLPLKK"
     gene            complement(20564..21937)
                     /locus_tag="ACHGMT_001363"
     CDS             complement(20564..21937)
                     /locus_tag="ACHGMT_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN6835113.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(21937..22332)
                     /gene="rbsD"
                     /locus_tag="ACHGMT_001364"
     CDS             complement(21937..22332)
                     /gene="rbsD"
                     /locus_tag="ACHGMT_001364"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFN6835114.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(22360..23277)
                     /gene="rbsK"
                     /locus_tag="ACHGMT_001365"
     CDS             complement(22360..23277)
                     /gene="rbsK"
                     /locus_tag="ACHGMT_001365"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFN6835115.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(23385..24398)
                     /locus_tag="ACHGMT_001366"
     CDS             complement(23385..24398)
                     /locus_tag="ACHGMT_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFN6835116.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(24479..25078)
                     /locus_tag="ACHGMT_001367"
     CDS             complement(24479..25078)
                     /locus_tag="ACHGMT_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFN6835117.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
BASE COUNT         7615 a         4536 c         5123 g         7807 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccgtat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatggttt ttcaccatct ttataataaa ctggttattt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcattg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaagaaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg attcgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atttgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt
     7081 cacaattatg gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt
     7141 gttttctcgg ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg
     7201 gaaaaccaac gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag
     7261 gaatatcgat ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata
     7321 tgagtctggt agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag
     7381 aagtacagca actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga
     7441 cactgaggaa tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa
     7501 tccaaggata tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat
     7561 ttttatatcg gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa
     7621 aattgaacaa actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg
     7681 aacaaatcag agaagtaatc gttttttttc agcctaaatg ggagaaagaa cagctagaat
     7741 tggatatcga acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt
     7801 ggctgaatat catggataac gccataaagt acaatcaaat caatggacag attcatatca
     7861 aactatttga gacagccaca gagattgtac tggaagtgac cgattcaggt gtaggaatga
     7921 atgaagagac cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa
     7981 tttctgggaa tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac
     8041 gtattgatta tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac
     8101 aataggaaaa agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc
     8161 gtttttttgt gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc
     8221 ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga
     8281 gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg
     8341 cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac
     8401 cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag
     8461 tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat
     8521 ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa
     8581 atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga
     8641 gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat
     8701 gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac
     8761 ttggtggggc ctggaaactt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat
     8821 tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt
     8881 tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga
     8941 tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt
     9001 attgttctac gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga
     9061 tttttctatg ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct
     9121 gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg
     9181 tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta
     9241 ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac
     9301 aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta
     9361 gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg
     9421 tttggcggac taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag
     9481 ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg
     9541 aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg tttgattgtt
     9601 catttaggta atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc
     9661 ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc
     9721 attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca
     9781 ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt
     9841 gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat
     9901 attttgttct tattggcaaa atttatttat ccattgacta caccagacat gatcttagat
     9961 tttaaagctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa
    10021 atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg
    10081 tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg
    10141 actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac
    10201 tatattatat gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg
    10261 acgtaatgca catccagatt ccaactgttc ttcttgatgg tactgtgcta gcagcattaa
    10321 gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca
    10381 gtgaatctac actatatccg gttttgagaa gattgaaaaa agaaggatat cttgagacct
    10441 atgatgaacc ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc
    10501 gtcatttaga agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat
    10561 tggaggagag cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt
    10621 cctgaagacg atcgttttga tttgatccag tattatgagg aatatttttt agattctgga
    10681 aaaacattag atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta
    10741 aaaatcagtt acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa
    10801 agacaaattc gcctgatttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt
    10861 ccactagcac tggggtttat attggttatt gggggactat tggttgcttt actagcgctc
    10921 atttttagta tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc
    10981 tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt
    11041 ctgttcgcca caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg
    11101 cttttttatg tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga
    11161 gatctgcatt cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg
    11221 attaccggta ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta
    11281 ggcgctcaaa agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag
    11341 acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg
    11401 gtcatacgcg gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa
    11461 ataaaggaga aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc
    11521 attagtttag aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta
    11581 tctgaattga caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa
    11641 aaagtgaata ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca
    11701 ataactgcga aagatgaatt aggctcactt cgattagatg aggccaatgt ggataacttg
    11761 atcgtcacag gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga
    11821 aaagtcatcc taaacggtgg caatttatct ttgcatgaga tggaaaacag tggctatact
    11881 atccaaacgt ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg
    11941 ttagaacaag gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg
    12001 acggatcaag aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa
    12061 aacagcaaag atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt
    12121 aaaataagaa agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag
    12181 aaggtcaaag agaaaagtta aaaacagaaa tggaaagatt acatacttat atcactcaac
    12241 taagccaaac cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt
    12301 cagaaggtcg ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga
    12361 agaaagagct ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg
    12421 ggaagaagga acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga
    12481 tggatctaga ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa
    12541 aattggatta tttaccgcag gcagccggag ggacacaggc aagcggttgg tacatcactg
    12601 aagacaaaga gttagaacta gaaggtgtaa tggcgcgtat tcgaaaaaaa aattaaaaaa
    12661 gtaacattag atatttattg aaggataaag gagcgcagga agtagcatga gagaaaaaag
    12721 agcattttat atcaatggtt atgcagggat tgtcggattg gtcgtattag ctttagttgg
    12781 attgtttttc ttctatttag gtatgtggca agcgaaagtt tgggcacttt ttttaagtat
    12841 ttttctttgg ttgatcacgt tattgttgtt aagttcggca actgtagtca gtcctaatca
    12901 agcaaaagta attttgtttt ttggacaata tttaggtacg attcgagaaa atggcttttt
    12961 ccttacgata cctttggcac aaaaaatgac tgtttcgttg aaagttcgga atttcaatag
    13021 ttctgtttta aaagtcaatg atttggatgg aaaccccatc gaaatctcag cagtagtagt
    13081 ctttaaagtc gttgatacag caaaggcatt attcgatgta gcgtattatc aagattttgt
    13141 agaaatccaa agtgagacag ccattcggca tatcgctagc caatacccgt atgatacatt
    13201 caatgagaat gatttgacgc ttcgaggaaa cacgacggca gtgtctgacg agttgcaaaa
    13261 agagctgcaa gagcgtttag cagtagcagg tgtcgaagta atcgaaacac gcttgaacca
    13321 tttagcctat gcaacagaaa tcgctagtgc catgctccaa cgtcaacaag caaaagccat
    13381 tttgtctgct cgacaaacca ttgttgaagg agctgtcaca atcactcaaa tggccttaga
    13441 gcaaatcgaa gatggacaag aaatcaattt caccgatgat cggaaagtcc aattgatcaa
    13501 taatttactt gtctcaatca ttacagacaa aggaacgcaa cctgttatca atacaggcga
    13561 catgaaagac cgtaagtgac atactttttg taagatagga agaaggagtt taggacggat
    13621 tattgtcttg aactcttttt ttagttgttt taaagaaaat gacaaaattg aaagaagaat
    13681 gatagttgat tcgaaggcag aattgttcgc ttttttctat aatcagtata taaagtaaga
    13741 gagaagggga aaaagtgaaa aaacttctga tcggtctact tgttttcatc gttgtggtac
    13801 ttgcaggggt gaaaatcgca gattacgtag ttatgggtgg agaagaatac tatgtacaaa
    13861 ttacgacaga tggtacaaaa acagtttcaa aatcagactc tggacaagaa acagtcaact
    13921 atagatatat cctgcctggc tatacgaaag agggtaagga aaaagaactg gacttcaagg
    13981 gacaattgac tcgaccgctt cgtaaaggag catatttaaa aatcacctgg aacaaaaata
    14041 aaggtgtcac ttcgtatgaa gaagtcgaaa aaaaagacgt gccgaaaaca gcactggaaa
    14101 aactaattaa agggtaggga aagaaatgaa caaaatagtc gaagtacaga atgtaacaaa
    14161 agtatacgga aaaaacaatg agaaaaagac gcaagcatta agcggcatca gcttcgatgt
    14221 agaaaaaggc gaatttattg ggatcatggg cgcttctggt tctggtaagt cgacactttt
    14281 gaatatttta tccacattag ataaaccaac agatgggcat atccgaatca atcaaaaaga
    14341 tgtgaccaca ttaaaaggaa atcaactagc agattttcgt gcgaatgaga ttggctttat
    14401 tttccaagat ttcaatttat tagaaagctt gacagcacaa gaaaatatcg ctgttcctct
    14461 ttctttacaa ggcgtcagac caaaagaaat caagcaacgt gtccaaaaaa ttgcagaacg
    14521 tttatccatc tctcatattt tggaaagcta tccttctgag atttccggtg gacaaaaaca
    14581 acgggtcgcc gccgcacgtg cattgatcac acaaccaacg attctattag gggatgagcc
    14641 tacaggtgcg ctggattcca aaagtgcccg ggatctttta gatacgatgg acgaattgaa
    14701 tacaaaagat catgtatcga ttcttcttgt gacgcatgat ccgttttctg caagttattg
    14761 ccaacgtatt ttattcatca aggatggcgg tatccatcaa gaagtgaaac gtggagatca
    14821 aagccgtgat tccttctatc gagaaatact atctattctt ggaaacttgg aacagtaggg
    14881 ggagattgac tatgctttgg aaattatcac ttactgggat caagagccgt ttaagagact
    14941 acttggtgtt attctcagga ttagttatgg catctgctat tttttacatg tttgaatcaa
    15001 tggcaagcaa cgaagctttt ttgaaaagta atacgatcgt tgcggctaca gttatcattt
    15061 tccgattcgg ttctgtttta ctagggatca tcacattcgt ttatatttta tatgcaaatt
    15121 cctttttgat gacgatgcgg caaaaagact atgcaatgtt tatgatgctg ggagcaaaag
    15181 gcagaaaaat cgcacagatg attttttctg aaacattctt agtagggatc gcagctactt
    15241 tagtgggttc agctgtcggt gtaggattga caagtatcgt ccaccgatta ttagtggatc
    15301 agttagatct caatatttcg cattttacac cttttagtat tcacgggttg ttgatcactg
    15361 ttttgttttt tgctgttttg tttttattag ctgctgttgt caatgctttt tcgattgtca
    15421 aaaaaccgat tttagcattg attcgtgcgg atcagacacc aacaagaatc aagcagcata
    15481 aattcttgtt tttccttgaa gtagtactgg gaattgcttt tctaggctgc ggttactata
    15541 tgatgaaaca cgtggggacc tatcaagtct ttggtatagg ggtagcctta gttacgatcg
    15601 tattaggaac ttatttcatt ttccattctg tgatcatttt ctttttatca ttactaaaga
    15661 aatcagaaca gatttcattg aaaaaattga acaatttcac attgtcacag ttaaactttc
    15721 gtattcgtga atatacacaa atgctctcaa tggtcgctat gctgtttgcc ttggcactgg
    15781 gcgctttgac ggtaggatta ggttttaaaa atgaaattgc agacttgaca gaaaacaatt
    15841 ctggatatga tctagtgatg aataatcctc gggcagaaga tcagcaaaaa atcaaagaac
    15901 tttcaccaac actttctgct tcttatactc aaaaagaaga tgcgggaacg atttactata
    15961 atgcaagtga atttgcggct caaccattga tcatgataaa acatgaagat ggaacaccgc
    16021 cgaagatcac gaaagaaaaa gttcctgcta aaaaaatgaa tgaactgact gtacaagagg
    16081 aacttcgaag ctatgaattg ccggaacagc aagaaaaaga aatcaaggta gtcagtcaat
    16141 cagaatttga tcaactgaac ttgccacaat ccactttaca gctagttcag gtaaaagact
    16201 tccaagcaaa cctgaaacca atcgaaacac ttgttgctca aaataaaatg aacaatccat
    16261 ctttgcaaaa agtagagtac agcaaccaga aatatgaaat gtatgaagtg tataatggtc
    16321 tattctctgg ttttgaattt atgggattct tcctaggttt agctttcttg accatgcttg
    16381 ctagctgttt gatgttcaag atcttgtcag gaagcaagag tgacattgct cgttatgaaa
    16441 tgcttgaaaa gataggaaca cgacgcgggt tgcttaaaca atcgatccgt agagaagtag
    16501 gcgtgttatt cttagcacca ggaatcttag gtgtagtcca tgtcttattc ggattacaaa
    16561 tgtttggtct attcatgcaa aatccataca aagatatttg ggttccgttt acgatattct
    16621 tcgtattgta tttcatctac tatgtactga cgacatggtt atatactgga atcgtattga
    16681 aaaagagatt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa gcaaaaatag
    16741 ctcctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    16801 ttgttcggaa aaagaggttg tgacaatatt tttgtcacaa cctttttttc acggtctttt
    16861 attagaatat agtaaatttt ttctcgttac tcacggaata agctgttaag aaaacggttt
    16921 ccatgtatac tggaatcagt gcaaatgaaa gaaaagaggg tgcaaggaac atggaaatca
    16981 atgaacaaaa ttttggcgaa aactatcact tgattacgat cacgaatcaa aatgggacat
    17041 ctttgtctgt cagtgatctt ggggcacggg ttgttcattt tcaaacaata atcgataatg
    17101 aaaaacgaga gttgatctta ggttttgatg aagcaaaaga atatctagaa aaagaccctt
    17161 atatcggtgc ttcaatcgga cgtatggctg gacggattac gaatggcaca ttcaaattag
    17221 atgatgaaac gttcgaagta aaaacagacc cggacactgg tcattgttta catggtggtg
    17281 cacctagctt tgaagccaag cagtggcatt atacagtcat caatggtgaa aacgaagcat
    17341 cggtcatttt ctatcagact agcccagata tggaaaacgg atttcctgga actttagatg
    17401 tagaagtacg gtatacgctg acgaacgatg atacatggcg agtgacggtc caagggatca
    17461 gtgaccgcaa aacagtattc aatcctacaa atcatgtcta tttcaattta accggtgatc
    17521 cagcacagtc aatcgacgaa catatacttt ggttgaatag ccgattattt gctacgctca
    17581 ataaagatac aacgcctaca ggagaagtag tttcagtaag tggcacagca tttgacttcc
    17641 aacgtcctaa aaaattaggt gaggtctttg ccagtgattt tcgccaatca atgatagtgg
    17701 gcggcatcga tcatccattt ttgttgaaaa aacaatctgg tttactaaat gctgcggaat
    17761 tgacaagccc agatgaaaaa gtatcgatcc aagtaaaaac agatgcttca ggaatcgtga
    17821 tttttacagc aaattttgga gaagatggac cggaaatgcg cgggaataaa ttagtaaatc
    17881 atggcggtat cacatttgag actcaagaac tgccaggagc ggagcgactt gaaagttttg
    17941 gagatgtcac tctggaacct aaccaagtac gcgagtttgt aacggaatac aaaataaata
    18001 taaagaaata gaataaatta aaaagggagt gtgacagtgt cacatcccct ttttaattta
    18061 ttctatttct ttaaaggcag atcaagccag aatgatttgt ctaaccttaa caaggttagt
    18121 acaggaacag ttgcagcttt aactgtacga gtagttgttt ctggtatctg tcttgccgta
    18181 aaattgttcc acaaatccca gattgaagcg acacggaaga tggaagaagc gttgttgacg
    18241 cgttttatcg ttgaaaattc taatcctgtg attggcacgc catctgttgt ccatttgatt
    18301 ggcacatcga atcgcttaga catattgagg atttcagcgt ttgcttcttc agttgttgct
    18361 gcttcttttt tcggttcatc atattttgtc aggtcgtacg cttcgatgat cgcgttcaat
    18421 ttttgtgaat attggcgatc tgtagcataa cgtccttcca aatatttggt tgcttctttg
    18481 tagtcttttg tttccgactt ccaagttggt ttgtagaaat caggattgcc agtcagtcca
    18541 tttttgatca agtcggcata gtcttccaaa ctttccttgt aagaaggata tttacggaat
    18601 tgtgcgcgga tttggtagct ttgtccggaa ctatcttgtt caagtgtatt gaaggtcacg
    18661 ctgcttcctt tatatgatcc tttgatacca aaaaggttat gatttggttc gcttgataat
    18721 aagctgtttc cagatccact ttcaaggata gcttgagcca tcatgactga tgcgtagatt
    18781 ccttcttttt gcccgatttc ctgtgcatca tctgcaatct ttttcaagaa ctgatcagtt
    18841 gtttggtttt gactgaaatg gaacgagcca tctaatccat ctaaaataga tgagccgcct
    18901 gtcagcgaac ctgaagcagc aggtaaacct gctaagttta ctcggacagc aaaatcaggc
    18961 gtgaaatctg aaatagaagt tactcgaact ttatctcctg gttgaggcgc atggataaat
    19021 tgtccgttcc caatatagat acctacatgg tgagtaacac cggaagaagg tccccagaag
    19081 acgaggtcgc caggttgggc ctgacttact ggaatctgcg tacctgcata ttgctgttcg
    19141 cctgtccagc caccgatata atgaccggtt gctttcatat atacgtaata agtcaaacca
    19201 gaacagtcaa aagtatttgg accttttgct ccccatacat atggttttcc aagatgcttg
    19261 aatgcttctc gaacgattgc ttgttggaca ttcgttgtat ttgtaggcaa tacagcatca
    19321 attggtgatt ttacaacaaa atcagaagaa gcagaagatg gattctctat accgctttga
    19381 tttgctgtat ttggacgaga tgaagcattg ttcgttccgc cattcgatga accattgttt
    19441 ggatggtttg gttctctctg agtatttcca ttgttattcg atccagatgg gtttgtttga
    19501 ttcgaagaat tcccgctgtt cgaattattg ttattattgt ttgaattatt tgaaccatta
    19561 ttattgttgt tattacccac atctggtttg tttggcgttt ccggatctgg tgtactaggg
    19621 acttcttcac taggatcagt tggtttctct gttgaagtat ccggtccagc tttaaattct
    19681 gcagtaatag ataaattgtt cgtacctact gtaaaggtct ttgcatcata atttacttct
    19741 ttggcaccat taacagcgac tgaagcaatt tgttcacctt cagctggcgt aatagtgtaa
    19801 gttaccttcg tttccgctaa taaaccttct accgttccac tttccgtaat atccaaaggt
    19861 tgctgttcag gggaatccgc tgtcgaagca gtcagttgga tcttaccaat caaagaagga
    19921 gcaattgtaa gggaatatgt ctcggctggc ttttcatttt ctgatggatc agccggatca
    19981 atttcagttg gctcggtcga tcctgttgtt gagttagttg ttccagttgg gtcttctgta
    20041 aaagaatccg ttgtttcact agtagctgag ctggatgtag tcgtatctgt tgtgactgtt
    20101 tgatcagcta tttgattatc tacggcaaat acggtattgg tcgacaaaac agacggagca
    20161 atcagccaag tagcagctag caaacgtatc gtttttttat ttttcataat aaactagtcc
    20221 ttttcctttc attgattttc tgtcttattc agcattcatt agacattttt tggaacagat
    20281 aatcgtgtcg aaaagctttt tcttttcata aatctgtctg tgtattctaa ataaaacaat
    20341 ttgtaaataa gcaacgagca ttcattcgtt tttctttgac gttcatttta ttgcatagac
    20401 cacctttgtt tggggataaa caagtgggga taaaaatggt tatgcatatg taaattatac
    20461 caaagtttgg aaagtatcac aaaaatttat aaaaataatg atcctaaaaa gaaaaagact
    20521 gaaatatgtg ataaacatct catacttcag tctttttcat taactattca taagacatta
    20581 ggtcttcatc aaacccgcta acgagagtag tgatcattgc tacgactaga ctaacagcca
    20641 gtcctgctaa gtaccagaaa aaagtaccgg aaatgaatgc aggcaatgtg gtcagactac
    20701 caaatacaaa agcgatcgct ttcacttggg caaatcccat tacagctcca ccagcagctg
    20761 caccaatgca agcagcaaca aatggttttt tcaatgggat cgcaattccg taaacagttg
    20821 gttcggtcac tcccgcaagt aaaatcgaag caaaagcaga accggagatc gaacgggttt
    20881 ttgaacgttt acttcgcaga aatacaccta aagctgcacc agccattcca aaattggctg
    20941 ctcctgtcaa cggaacgatt cggtcgaacc cataagcaga gatattactg atcatgacag
    21001 ggtttacagc ccattggata ccaaatatta ccaaaatatt ccaaccacca gcgatgattg
    21061 cgccactgat cggtccgtta atatccatca tccaagtaat tgcggcagct aaaccttcgc
    21121 caaggtaaac acccgcaggc ccgaatacca ttaacgtcaa aggaaccgtg ataactaatg
    21181 aaagtaaagg tacaaatact aattgcatat tttcgcgaat atattttttg aggaattttt
    21241 caacaaaaga ataaaagaaa atacctaaaa tcgccggcat gacagtagac gtatactgca
    21301 tcagtactac aggaatcccc agaaaatcag tagctgcttt gccactgtct gaaagtaaag
    21361 aggtcagatt cggttcaatc agtgctgcag caatgacagc cgcaatatat ccattcactt
    21421 taaacgtttt ggcagcagaa aaagcaagaa agattggcag aaaataaaag acggcattcg
    21481 acgcagcaga caaaatatga taagttccgc ttgtttcact aagccagcca atcgtagtcg
    21541 ctaaaacaac cagacctttt aatataccag aaccggcaaa cagcggaatg atcggcgtaa
    21601 agatttccga tatcaaacca aatagttttt cgatcggatt tagcttttct ttcggagctg
    21661 aatgatcgat ttgacgcgtc aattccttgt aagcaggagc caatccctga tccatgatta
    21721 tttctgcata tctatcgagt aaaatgaaat acttaatgga agggatttgc tgttttaatt
    21781 tttctgcatt ggctactggc tgttttttca atttgatacg aagccgagtc gcacatggaa
    21841 ggacagaatc aatattgtct tttcctccca actgttcaat cagcatatga atctgctcgg
    21901 acagtaccat ctgttcagga ttcattgtcg tttccattag aagaccactc cactcactaa
    21961 tataatattg gcgtaaggtg tcatttcgcc tgtacgaata aatgcatgac agttattcaa
    22021 ctcttttttc atttcttcat gtgggataaa gctaatcggt gtatctggta acaataattt
    22081 gattttttcc aatatttccg ggttcattgt ctttatttct tccgccaatt caatcgattg
    22141 tacctcaagt tcttctaaaa cgttttctaa aacagataaa aaacttggaa gaccgtttga
    22201 gacagccaaa tctatttttt cagtaccaaa ggggacaggc atgcctgcat ccccgatact
    22261 taatttatca aagtgcccca tttgtgcgat tactcgtgaa atatctgaat tgattacttt
    22321 tccttttttc atgtttgttt cttcctctca ttgttaattt tagactgctg attgttctaa
    22381 agcttcgcgg taagggatag aaggttgcgc accataagac tgcacagcaa cagaagaggc
    22441 tagatttcca taaaggatcg cttctttcaa gttactaagg tctggctgaa gtgtgctgct
    22501 taatgcgcca ataaatgtat cacctgcggc tgttgtgtca acggcatcca ctttaaaagc
    22561 agggacgata ccgtgttctt tttctgtatg atagaaagcg ccggcactgc ctagtgtaat
    22621 gattactgtt ccaataccta attcatggag tttttcggct gctgcgacca aactgttatg
    22681 atctgtcact ctgattcctg taatgatttc tgtttctgtt tcatttggta taatgatatc
    22741 tgtcttttca agtagttcag taggaatcgt ttcacgagct ggtgcaggat tcaggatcgt
    22801 tgtttttcct gcgtcttttg caatagaaaa agctaggatc gtcgcatcta agctactttc
    22861 aaactgagca ataataaagt cactgtttgt aatcagttgc ttgtttttta ggacttcttg
    22921 ttcatggaaa gcattatttg cccctgcatg gatcaagata ctattttctc ctgctgcgtc
    22981 taccataatc attgcttgtc cggttgtggc gccttggatt tcagctatcc cagatgtatc
    23041 gatgttttct tttgtcagta gatctaataa ctgttgtcct tcgctgtcag ccccaacgcc
    23101 tccaataaaa gatgtccgtg cgcctgaacg tttggctgcc actgcttggt tggcaccttt
    23161 acctccgcca gcgtgaaaaa tctcatgcgc atgcatcgtt tcgcctggct tgggcatgtt
    23221 ggttaaacga agggtagtgt ccatattaat acttccaatt actgtaaccg tgttcatttt
    23281 acaaacctcc aactatctca tttattttga tgggaaaatt attttttaaa acgttttagt
    23341 aaaacgtttt aatagttcga taaaaatata gcacaccttg aaagctattt caagggtgca
    23401 gttgaaaatc ttttttctaa tcgtacaggt aatctttttt cttcccattc tttttctggg
    23461 aattggatcc gatctaacaa taacttggca gatgtttgtc ccaattcgaa gattggttgt
    23521 gcgatcgttg tcagtttcgg cttgatatac tcgcacatat ctatattatc atagccaata
    23581 atgctgtagt cttcagggat gctttttcca gcttcctcta atccgcgata taatccaaaa
    23641 gctaattcat catttaaagc aaacacacca gttgcagcag aatcaagaag ttctgctgtg
    23701 atctggtagc caccttgttt tgagaactgt gtaggtaaga ggatcagttg gtcgtgaggg
    23761 atttggtaaa catcaagagc agatttaaac ccctcaatcc gagcgtggac attttccggt
    23821 ggattttcag gatagacaag ggcgatcgtt tgatgtccta gactaagcaa gtgcatgccc
    23881 gctaggtaac caccgcgaaa atcgtctgtt cgtacggcat cactaaatcc ttcagatttt
    23941 ttctgatcca aaacaatgaa gggccggcct tgttttttca aattttcgtt gattgcatct
    24001 gtagagacag cagaagtcgc gataatgaat ccatccacac ctcgacgtgt gagctcggct
    24061 agatattcga tttctttttg atgatcagaa tcagcattac ataatatcgt gacgaaattc
    24121 tgcttataca agatatcctc aattcctcgc atcagtgtac tgaagaatgg attcgtgata
    24181 tcaggaacga gtacaccaat tgttttcgtt tccttggtaa tcatttgtct agcaaaatag
    24241 tcaggctgat agcctaattc ttctttggca gctaaaactt tctgaacagt tttcggactg
    24301 aatttctccg tatttccatt taatatcaag gagacagttg tgattgaaac gccggaaagt
    24361 ttagcgactt ctttgattgt gatctttttt ttcaccatgt tcatactcct atctaaatcc
    24421 tgtggttatt ttcccatgaa atcaaaaaga agtaaatgaa aatcacgtgt gccagtactt
    24481 atttgctttg ttggatagct tcgattattt gtttcgttga gcgtactcgt gcagttttcg
    24541 gaaatatctt ttttatgaac aattgatggg attctttgtc acgatcactg caagcatctt
    24601 cagcaagcac gatatggtac ccgtgttgaa aggcatctaa tgcagtagca tatacaccgt
    24661 ttgttgtcgc tacaccgctt aaaatgatag tttcgattcc acgccggcga agttgaagat
    24721 caagagaagt accaaaaaaa gcacttggat tgtattttgt gatagtcaac atattatctg
    24781 tatctttcaa agaatccttc aaaagtaaat cagtgaattc tggaggtaca gaagcagtca
    24841 ttccacccat atcagtttgt tgactcaaat aagggaaagt ggagatgtct acgttcacta
    24901 aaactttcaa ggcagcggta cgtttcaatg ttcttactaa caaatcattg tttgtcagaa
    24961 tagatgctgc agtgtgtgga gagagtgtac cgttatttac gatctctttt tgcatgtcga
    25021 tcgcgacaaa agcagttttt gataaatcaa aaatcatatt ttcctttaaa tcattcatga
    25081 t
//
LOCUS       DAZBSA010000037        24260 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000037 DAZBSA010000000
VERSION     DAZBSA010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.037"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..1339)
                     /gene="purB"
                     /locus_tag="ACHGMT_001368"
     CDS             complement(44..1339)
                     /gene="purB"
                     /locus_tag="ACHGMT_001368"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFN6835118.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1352..2476)
                     /gene="purK"
                     /locus_tag="ACHGMT_001369"
     CDS             complement(1352..2476)
                     /gene="purK"
                     /locus_tag="ACHGMT_001369"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFN6835119.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2469..2957)
                     /gene="purE"
                     /locus_tag="ACHGMT_001370"
     CDS             complement(2469..2957)
                     /gene="purE"
                     /locus_tag="ACHGMT_001370"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFN6835120.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3373..4680)
                     /locus_tag="ACHGMT_001371"
     CDS             complement(3373..4680)
                     /locus_tag="ACHGMT_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFN6835121.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4683..5264)
                     /locus_tag="ACHGMT_001372"
     CDS             complement(4683..5264)
                     /locus_tag="ACHGMT_001372"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFN6835122.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5380..5477)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5836..7128)
                     /locus_tag="ACHGMT_001373"
     CDS             complement(5836..7128)
                     /locus_tag="ACHGMT_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN6835123.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7125..7748)
                     /locus_tag="ACHGMT_001374"
     CDS             complement(7125..7748)
                     /locus_tag="ACHGMT_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835124.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7751..8587)
                     /locus_tag="ACHGMT_001375"
     CDS             complement(7751..8587)
                     /locus_tag="ACHGMT_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6835125.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8604..9491)
                     /locus_tag="ACHGMT_001376"
     CDS             complement(8604..9491)
                     /locus_tag="ACHGMT_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6835126.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9507..10790)
                     /locus_tag="ACHGMT_001377"
     CDS             complement(9507..10790)
                     /locus_tag="ACHGMT_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN6835127.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11473..12156)
                     /locus_tag="ACHGMT_001378"
     CDS             complement(11473..12156)
                     /locus_tag="ACHGMT_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFN6835128.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12174..12791)
                     /locus_tag="ACHGMT_001379"
     CDS             complement(12174..12791)
                     /locus_tag="ACHGMT_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFN6835129.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12801..13625)
                     /locus_tag="ACHGMT_001380"
     CDS             complement(12801..13625)
                     /locus_tag="ACHGMT_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFN6835130.1"
                     /translation="MNKFLGFRAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13618..14178)
                     /locus_tag="ACHGMT_001381"
     CDS             complement(13618..14178)
                     /locus_tag="ACHGMT_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835131.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14352..15308
                     /locus_tag="ACHGMT_001382"
     CDS             14352..15308
                     /locus_tag="ACHGMT_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN6835132.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15613..16569)
                     /locus_tag="ACHGMT_001383"
     CDS             complement(15613..16569)
                     /locus_tag="ACHGMT_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFN6835133.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16673..17275
                     /locus_tag="ACHGMT_001384"
     CDS             16673..17275
                     /locus_tag="ACHGMT_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFN6835134.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17363..18862)
                     /locus_tag="ACHGMT_001385"
     CDS             complement(17363..18862)
                     /locus_tag="ACHGMT_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFN6835135.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18878..19402)
                     /locus_tag="ACHGMT_001386"
     CDS             complement(18878..19402)
                     /locus_tag="ACHGMT_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFN6835136.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19700..20449
                     /locus_tag="ACHGMT_001387"
     CDS             19700..20449
                     /locus_tag="ACHGMT_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFN6835137.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20440..21225
                     /locus_tag="ACHGMT_001388"
     CDS             20440..21225
                     /locus_tag="ACHGMT_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN6835138.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21194..21910
                     /locus_tag="ACHGMT_001389"
     CDS             21194..21910
                     /locus_tag="ACHGMT_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN6835139.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22012..23031)
                     /locus_tag="ACHGMT_001390"
     CDS             complement(22012..23031)
                     /locus_tag="ACHGMT_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFN6835140.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23050..24051)
                     /locus_tag="ACHGMT_001391"
     CDS             complement(23050..24051)
                     /locus_tag="ACHGMT_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFN6835141.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         7188 a         5221 c         4225 g         7626 t
ORIGIN      
        1 ttttgctatt ctattttaat tgaattttgc aactcactct tctttattcc aaacctactc
       61 gtttgaatat cacgtcaacg tttttcaaat gatgatgata atcaaaggca tcgtctattt
      121 cttctttaga taataccgat gtgatggttg gatcttcttc taacagcggg cggaactgca
      181 cttggttgtc ccaagcgtac gcagtttttg gctgaacaag atcataagct tcttctcttg
      241 tcattccctt atcgatcaat ttcagcataa cgcgctggct ataaatcaaa ccaaatgttg
      301 cagacatatt ccgcttcatg ttttcaggaa agaccgttag atttttaacg atattgccaa
      361 aacgatttaa catgtaattc aataaaatcg tggtatcagg caagatgatt cgttctgctg
      421 aagaatgaga aatatctctt tcatgccata atgtgacatt ttcataggca gtgaccatgt
      481 gtccgcgcac cactctggct agaccagcca tattttctga accgatcgga ttccttttgt
      541 gcggcatagc agaagaacct ttttgacctt tcgcaaaaaa ttcttctact tcacgtgttt
      601 cggatttttg caatccgcgt atctctgttg caaaacgttc aatacttgta gcaataagcg
      661 ccatcgctga gaaatattca gcatgtagat cccgagggag aacttgagta gagatttctt
      721 gtggacggat ccccaattgt ttacatacat actcttcaat aaatggcggg atatttgcga
      781 acgttcccac tgctcctgag atttttcctg cttctactcc ttttgccgca tgttcaaatc
      841 gttcgatgtt ccgtttcatt tctgaatacc atgtggcaag tttcaatcca aaagtagttg
      901 gttcagcatg gacaccgtgt gtccgaccca tcatcactgt atatttgtgt tcttttgctt
      961 tttcgccaat gatttccgta aaacgtttca agtcttcccg taaaatatca tttgcttgct
     1021 tgatgagata gccataagca gtatctacga catccgtact tgttagacca taatggaccc
     1081 attttctttc ttctcccaat gtttcagaaa ccgctcgtgt aaacgcaacg acatcatggc
     1141 gtgttaattt ttcgatttcc aagatccgat cgatatcaaa tgatgcatgc tcacgaattt
     1201 ttttcacatc ttcttgcggg atttcaccaa gttctgccca agcttcatct gctaaaatct
     1261 cgacttcgag ccacgcacgg tatttgttct cttccgtcca gatattcccc atttcagggc
     1321 gtgtataacg ttctaacatg ttttttctcc tctaatccca aatattcgtt tgtttgattt
     1381 cttccagcgt ttgctggatg tcttctgtca agatagtgat atgtcccatc ttgcgacctt
     1441 tttttgcttg tttttttcca tagaaatgga attgccagtc tggcttattc tcaatcaagc
     1501 gatagctttc cagcagttgt tctcccaaaa cattgaccat tactgcattg cttatcaaac
     1561 gtactttttc tcctaatggc caaccacaaa tacctcgaat atgggcatcg aactgactca
     1621 tagaacaagc ttcaatagaa taatgtccag aattgtgagg acgaggagct aattcattca
     1681 catatagccc gcctgttttt gttaaaaaca tttctacccc taaaacacct gaaaggctca
     1741 ctgcttctgc aatcacacgg gcaatacgct cagcctcctc gatcacatcc tgatcaatcg
     1801 cggctggagc aatcgtttca tgaagaatat tgttgtggtg gatgttttcc acgaccggaa
     1861 aagtcgtgaa atctccttga ccatttcctg ccaccatgat cgaaatctct ttttcaaacg
     1921 ggatccatgc ttctaaaaca caggttcctt ctcttaaaag gtccatggca ggagctagat
     1981 ccgcccgact tttcaaaaca tactgcccct tgccatcata gccgcctcga gtggttttca
     2041 aaacacaagg atagccgatc ccgtcaatcg catcttggat atccgtcgga ctgacgatcg
     2101 ttgcataagg agcaatcacg atgttattcg tttccaaaaa agatttttct aacaggcggt
     2161 cttgcgtaat cgcaagcaag tctgtccctt gaggaataaa tgacatgggt aaaatcgcat
     2221 ttaacgcctc tacactgacg ttttcgaatt cataggtaat gacatctgtt cttctggcaa
     2281 gctctttcag cgcaaaggtg tcatcataga cactttcaat gtgccaatct gcaacttgtg
     2341 cggctggaca atcgctcaca ggatccagca cacctacttt aaagcccatt tcttttgcac
     2401 taatcgccat catacgacct aattgaccgc caccgacgat cccgatcgtt gaaccaggca
     2461 acaaaggttt aaccaagttg atcactactt tctataacag ttttagccaa cattgaacgt
     2521 ctttcagcca atttagcctc caattctaaa tcatacatgg aaagcatctg tatcgcaagt
     2581 agtccagcat taattgctcc cgcttttccg attgctgtag tagctacagg tactcctcca
     2641 ggcatttgga caatcgaaag aagcgaatcc aaaccattta atgttcgtga ttggaccggt
     2701 acaccgataa ccggcaaagt tgtttttgat gcgaccatac ccggcagatg cgcggctccc
     2761 cctgctcctg caataatgat tttgattcct ttttttcgag cgttttgtgc atattcaaac
     2821 atcaaatctg gggttcgatg agcagaaacg accttttttt cataagacac accgaattca
     2881 tccaatattt gacatgcttc tttcattgtt tcccaatctg aagtactccc cataatcaca
     2941 gagatttttg ctgtcattgt ttttcctccc agtttttcta cattcatatt ttaacaatca
     3001 ctttttttga tgtcaaagaa atttcgaatg ttaacaaaga aaaacatttt attgttcgtg
     3061 tttattaaag ataaaaaaaa ggaatgtgac aaaagttagg tcacattcct ttttatggat
     3121 aaacggtgtt ctcctgccaa ctcctcggtc acaaggaaca atattcaaca aacatgagaa
     3181 gctttttttt gaaattgttc cttgtgactc ggagatgaac ggcaggctca cgacctcttc
     3241 cttcaacggt gttcaatcag ctgaccctcg gtattagctc aaacaaacgg caaaatacga
     3301 ggaactattt ttccgtttgt ttctctaaca atcaggtcaa aacgctgatt tcacaacctt
     3361 tcttttgctc tattacttct tgaaggcttc ttccaatatt tcttcatctt ttttcaatcc
     3421 attaaagatc aaattcaata tgatagccgt aatgctactc attacgatgc catttccagt
     3481 gaacatttgc aatgtttgcg gcatttgact gaataatgtc ggcgtaacat tgaaacccag
     3541 cccaaatcca acagaaacag caacaatcaa tagattttta tcattgctga aatcaacttc
     3601 caacagcata cggattcctt ggactgccac catcccgaac atgactaaca ttcctccacc
     3661 aagtaccggc tcagggatga tttgtgctaa cgcaccgatt ttagggaata atcctaaaac
     3721 aatcaagaag aatgcagaga aatagatggg ttttcttgtt ttgattcccg aaagttgaac
     3781 caatccgaca ttttgagaaa agccagtata agggaatgta ttgaaaatac cgcctaagat
     3841 aacggcaagt ccttctgcac gatatccctt tttcaattct tcttctccaa catttttccc
     3901 agtaatgtca ccaagtgcaa aatatacacc tgttgattct accatgctga ccatggaaat
     3961 gatgatcatt agtaaaatcg accatacgtc gaaagttggt ttaccaaaat aaaagggttg
     4021 tggaacatgg aacaacggcg catctccaat cgcgctcaga tcaatcattc ccatgaaagc
     4081 agctaagatg cttcctccga ctaaaccaat caataccgcg attgaacgaa tgaacccttt
     4141 tgccagcatt tgcacgccta caattaggag aatcgtcaca aatgcaagta gcagatttgt
     4201 tttgtcacca aaatcagctg ctgttgcatt acctccaccc attttctcga ttgctacagg
     4261 aatcaaagtc aaaccaataa cggtgataac cgttcctgtt actataggag ggaatagttt
     4321 tttaatttta gaaaagacac cagaaactaa cacaacaaag atcccagaag cgataataga
     4381 tccgtagata gctcctactc cttcattggt tccgatcaag atcagtggag ctactgcttg
     4441 gatcgcacat cctaaaacga caggcaagcc aattccgaaa aatcggttaa ctgtcaattg
     4501 caataatgtc gctaatccac acataaagat atcaattgaa atcaaataag tcatctgttc
     4561 ttgattaaag cctagtcctg taccgatcaa aagaggcacc gctactgctc ctgcatacat
     4621 cgctagcaga tgctgcagcc ctaaaaccgc agcttttcca ttttgtgttt ctttttccac
     4681 gattatgcgt cctcctcaag aaactcaact tgtccattcg tcaaggaagc aatacgcgca
     4741 agagaaacga ctctcaatcc catttcttca agtaactgac gtccatcttg gaaagacttt
     4801 tcgatgacga tcccgattcc ttctacttgc gctccagctt gctggcataa ttcgatcaat
     4861 ccttttgccg cttgtccgtt tgccaagaaa tcgtcgataa tcaatacttt gtcttctgaa
     4921 gataaaaatt tacgagaaat agaaatcgta cttgtcactt gtttcgtaaa agaataaaca
     4981 gaagctgtca ataattcttc atccatcgtc aaactttttg cttttctagc gaagatcatt
     5041 ggaacatcta attctcttgc agcaaataaa gctggtgcga tcccagaagc ttcaatggtc
     5101 acaactttcg tgattccttg ttctttaaat acttctgcaa accgttttcc catttgttcc
     5161 atcaatacag ggtctacttg gtgagtgacg aagctgtcga cttttaatac gccctcgcct
     5221 aatatacgtc cgtctttttg aatacgttca actaattctt tcactttatt tgctccttta
     5281 tcatttatta ataccgaaat aaccgctttt gtgcaaaaac ctactatgtt tctgttaaag
     5341 aaaaacaccc tccactttat cataagaaaa gaagggtgaa atagccaatc ttttacttat
     5401 agtccagcat ttacggtact ggctagaaac tgtcatccat attatgacga tatataagta
     5461 ggtatgtaaa actaataata gtgtaaattc attttcttgc aactgttttg cacgtgaagg
     5521 cagtttgcca tatttgaata aaaaatgttg tttcaaatat tgaaaacagt ctaacactac
     5581 tttctgaaac tgacaacgtg gtttacttat ttttttgtaa aatctaatga tttttaatgc
     5641 attattcctt tttgttcggg tttaaatagt tatttttatt ttctcctatc taaaaaagca
     5701 gcccacataa aaagaaggca tgacaaaaag catgtcatag cccctttttc cgaataaata
     5761 gtgttcaaaa gacagcctat cggcaataag gcaagatttt ataaactacg ggaagtaatt
     5821 tccgtaaatc tattcttatt cttttaagct gacaagctcg tcaatcacta ctgcttttcc
     5881 tgaagcaata gaatgatttg ctgcgattcc tgtcaaaatc gacaaaatac catctacgtg
     5941 agttgctgcc cgattccacg gatcttcttc tttttcttca ccaaatagat catctaataa
     6001 acgcggatct cctccgccat gtccgcccgc ttcttgttcg acgattacct catatggctc
     6061 accgaacatc ggatagacat ggatggactg aagaacggct gctccttcat cctttttatc
     6121 tccacctgca ttgatgtaag acttttcgat cactttcatt tcgatccgtc ctttgctgcc
     6181 attgaaagaa acattgaatc cttcccacgg catatacgcg tttagcgaat acgtcaaaat
     6241 cgcaccagtt tcatatttga ccgttacgcc tagtgtatct tcaatagaga tatcatcgga
     6301 aaatacactg cggtcacgaa tatatccact gtctttttct gcttctaaat acatttctcg
     6361 caattcctca ttggctgaca ggtctatagc aaatggatca gctttagcta gttcgcttcc
     6421 atagcatcga ctgtaaaata tttctactcc gcgtttctcc gcattatgca tcccataaaa
     6481 gtttaatcca ccgaatgcaa ataccttttg gggacggctg ttcaaccaaa aattcactag
     6541 atcaaaatga tgggtagatt tatgaacaag aagcccgccg ctattgtgct tatttcgatg
     6601 ccatcttctg aaataatctg ccccgtgttg cgtatttaac agccattcga agtggataga
     6661 aaacacatca ccaatcacat cactttctat cagttccttt atcttcgtgt gatacggtgc
     6721 ataacgataa ttaaaagtga ctcgcaggct tcttcccgtc tgtttttgga ccgttattat
     6781 ttcttgagct ttttcctcat cgatagtcag aggtttttcg caaataacat catatccttt
     6841 tttcattgca cgaatgatat aatcatgatg cgttcgatct actgacgtaa taatgacttc
     6901 agccggccgt acgtcatcca gtagttgctc aaattgtgat ggcatacatg tctttacttt
     6961 tttctgtcca tacttgttca caagtatccg atttgcataa tccatccttg tttgagatac
     7021 atcacaaaaa ccgactagtt cacaagtttg atgatacgta gaggcaattg cctcataaaa
     7081 aaaacgcgct ctcccaccaa gtccgacttg tacatatttt ttcttcattt aaagtcattc
     7141 tccttttctt ttaagcaaaa tactgtgaca aatggcagcc ctggatacag taatgcagtg
     7201 agtaaaaaat catgctgtat acaccagaac agtccataca aaaggcataa ataccacaga
     7261 aaccccttga cgttttttga caacagccag atattccttt tgatcaaata aacaagacta
     7321 tctgctttat cttctttgca gatatcaatt agcgtactaa aaaaactatg ggatacaaaa
     7381 agcaacagac acaatagaaa aggaatcaga taccaagctc caatctttcc cactaataaa
     7441 taccaatcaa ctgcaaaaat aatgacaaac agctgaaaca acgtaatgat gaaacacatc
     7501 cgtttgattt ttcctttcct ccaacatacc tttttttctg ccaagtaatg gacagataga
     7561 taataagaag gatacatcat caaaaaataa atcagataga caacacgagc agacagcact
     7621 aatcctaaag tgaacaaaca tcctagaact aaagcgaaaa gagcattact gataagactg
     7681 gttaaaaaaa tccgacagac atagtagttg agcttgtaaa gaaacatctc ttctattggc
     7741 tttttcatat tcagcccttc agaccgcttg tactgatccc ctggacaaga tatttattga
     7801 agaataagaa aatcagaaaa accggtagca gcgacaacgt agacatcgca aacatactcg
     7861 cgtaagtcgt actagatcca gcgtctgcaa ataatttgat ggcaatcgat accgtgtaag
     7921 atctcggctt gtttaaataa atcaatggtc ccatgaagtc gtcccatttc caataaaact
     7981 ggatgatgat cgtcgtgatg atcgaaggtt tcagcaaagg aaaaataatt ttggtaaaga
     8041 cactatactt gctgcatccg tcaataatcg ctgattcatc taactctttg gggatgctga
     8101 ccatgaattg catgatctgg taaatgaaaa atgcttgtcc aaaaaaatgt ggtaaaatca
     8161 gcggcacata cgtcccaacc aaattcagcc gattgtaaat aataaactgc ggaatcaaaa
     8221 tcacttgtgc aggaatcatc atcgttacta acatgacgac gaaccagaat ctttttcctt
     8281 tgaaatcgat tctcgataat gcgtaagcaa tacaagtcga gctcagaact gtccctatcg
     8341 tcgaaactgc agagatgaca aacgaattcc taaagaaagt aaaaaatgaa attccagcaa
     8401 accccgccca tccttttgcg taattttccc attgaaaagt ctctggaatc aaattcagcg
     8461 aaccactcat aatctcttga tccgttttaa aagaacctgc gatcatccat aagaccggat
     8521 aaatcatgac tagacctaat agcaaaataa aaccgtattg aaaccatttc gttactgtat
     8581 tattcatttc ttcctcctct cattcacaca acgacccatt tttttgatga tttgaaaatc
     8641 agaaaggtga cagctcccat aatcaacagc atgatccacg aaatagcact agcatacccc
     8701 atagaattaa aagaaaaagc ttggttatac acataaagag cataaaagtt cgtggcatca
     8761 ttcggaccac cttgggtaat gataaatgcc tgcgtaaaag tcataaacgc cgcgattgtc
     8821 tgcataacca agttatacaa aatgatcggc gaaagaagag gtaacgtgat cttgtaaaac
     8881 gtctgccatt tgttcgctcc atcgatctct gccgcttcat aatacgagcc tgggatctct
     8941 ttgatacctg cagcaaagat gatcattgcc gaaccgaatt gccaagcaga catcaaaaca
     9001 agagggatca tgaccagttt ttgatcgcta aaccaggaaa gcgcaggtgc gcctaatcgt
     9061 tccaggaaaa tattcactaa cccgttttta gaaaacaact cccgccaaac tagagcgata
     9121 gaaatactgc caccgatcaa actaggcaca taatacaaag aacgaaagaa agtcgttcct
     9181 ttgatattcc tcgttaaaag ataagcaatc aatagaccaa agatcagttt cacaggtaca
     9241 gatgcaaata catagagaaa agtgactcgg atggagttca aaaaacgggc atcactcagc
     9301 atctttatat aattatccaa gccgatccat gagactggat ttcccaaacg ataatcggtg
     9361 aacgaataat aaagtgacaa gatcatcggt atcactgtta tcaatagaaa tcctaggatg
     9421 aatggagaag caaatacgta accgaccaca ttgtcttgat ccaagaaccg ttttagtttt
     9481 tcttttttca tgccttcctc ctgctgctat cctagaatgt ttgacgcaaa ctcataaaga
     9541 ctctctgctg cttcttctgc tgttacttct tcataaatga ctttttcaac taaaagattg
     9601 tattggtctt gaatttccgg attcaaagca ctatcaaaga cattcacttc gccgttgtcc
     9661 atatcggaaa tcatttctaa ataatcaaat gtcgcttgca aagttgcatc atctgcacga
     9721 gcgagctgtt ctcgaatatt cgacatgatt ggtacaccac gttctccttt taaaatttca
     9781 ttggcttctt cactgttttg aaagaaatca ataaacttag cagcttcttc tttatgggct
     9841 gagctttttg ccatactcag catttgcgaa gaacgtaaag ggataccaga ttctccatct
     9901 gcttttctgc gaggtggatt aatcaactga atctccatat cttcaggagc ccctgacaat
     9961 atttctgtca actggttact tgatagaaac accattgccg cttctctatc cctaaccgcc
    10021 tgaaaagaca gatccgtcgt acttgcagct ccaccacctg gttctggatg ggcccctttg
    10081 tctaccagat ttttacgcag ctggaaaaaa tctgttagat agtctggatt ttcaaatccc
    10141 aagctgttag atagatcttc ccgattataa aaattcaatc ctttctcaac ttgtggtatt
    10201 tgaataatac agccagctat aaaatcttcg aatcttgaaa tagcatagat tcctaattct
    10261 tcagtgattt gatagctgat ttgttcaaat tcatcccatg tccaatttga agctggttca
    10321 gggatattta attgttcaaa tatattttta ttgtaagcaa tcgcaaacga attcaccccg
    10381 ttagacagtc caactaattg cccgtcgtaa gtcgtcgttg ctaaatagga ttcatctgca
    10441 ttagaaatat caattatccc ttgctccaca tattcatcta atggttcaat ctgattgatg
    10501 tattgaggaa attgattgtt cagttggaaa atgtcccaga tatcatctga tgctgctagt
    10561 gtacctaatt ttgtgtaata tccttcataa tcataaaatt cataatcgat cgtcacatgg
    10621 ggattttttt cttcataaag ctcgattact tttactgttg catcgtgtcg gacttgcgaa
    10681 ccccaccaag ccatgttcag cgtgattttt ttgtcatctt cttgcccaca agccgtcaag
    10741 aagaataaca taacacttaa agctaagatg attctcattc ttttcttcat tctctgcacc
    10801 tctacttcca ttgttatttt tcaatatact gtatttttat acagaacata tttattaaac
    10861 gatctttttc aaggggaata aaaaccaaga cttgccttat cgttctgcct aggatgaaca
    10921 actcaatact ttcaagaaaa attagatagt gctgattctt ctttcatcgc taaaaacact
    10981 tcttgcttca atgatgggag gataattacg caaagaaaaa aggatatcag acaactgccc
    11041 aatatccttg acatagctaa ataagcatgc ttcttcgtgt gcgctcttcc ctaaaaaaca
    11101 gaaaaataac tagtaattct gtatgaaatt aggtacaata tacacgtcga tgcacaagca
    11161 ttgactatgg caacttgatt ggtcagttcc cgctgacttt tcaagccccg acaatttagc
    11221 tgctactaaa ttgtcgggtg ccttttttag tttttattcg tttttcattc tatagaaagc
    11281 gcttcaataa atcaagacat ttttttgtta gaatgctaat aataacaaaa ggttttaact
    11341 cttcttatgt acattacagg taaaaaaaca aaaaaatttc tctttttttt gaattctgaa
    11401 caaatagtac tggagtacaa aagacgagga agagccacta tctgctcttc ctcgtctttt
    11461 gattctcacc tgttattcat gattcttttt ctcagtttcc cgatattttt ccattttacg
    11521 actaaaaagc tctccgctac ttggtggcgt atatgtgatg gcatttgctc ctgcttgaat
    11581 ggtctcagca atactttctt ccgttggacc acctgtcgca atgataggaa gttcaggata
    11641 ttttttacga aaatggcgta ctgcttctgc agtttcttta cctgcactga tattgataat
    11701 cgaaatacca gctgctaact tttcatctat ttctgtatat ttcgacgtta ccgtactgat
    11761 aactggaata tcaactactc gacaaacttc ttttactgtc tctaaaggcg taggaccgtt
    11821 caaaacgacc cctaacgaac cttgcgcttc tgcgaacatg ctcatgtagg aagaacgagc
    11881 accttgtgtc aaaccaccac ctacaccgga gaaaactgga atgtcagctg cctcaataat
    11941 gcttttagta atcgctggat gaggtgtaaa aggatacaca gcaattactg cattcgcatt
    12001 tgtattacgt atgatcgcaa tatccgtagt aaagatgatc gaacggatca attttccaaa
    12061 tatcttgatc ccgcttgctt ctaaaatgat ttccggtact cttacgatat cttttctcaa
    12121 ttccgtcatg atttctggcg taaattgttc tgccatcagc cacttctcct ttgctatctc
    12181 cggtcatact gattcaattg atacgtctcg atgacctgga tgattttttg cgcataaccg
    12241 gggtctgtcg cataacctgc atcttgcaat gcttgtgcgg cttcctgata gttggtggcc
    12301 gtaatcactt tttcatacag cgcaggattc caatctactc cttggacgaa cagttgtgta
    12361 tggtcatcca tggactgttc ccatgaatca tacactttga agtctccttg gatagttatc
    12421 cattcttcgt taacaaattc ctttgtttcc aggcttactt tcttctgatc cccataagcc
    12481 ttgatcccga aaagattatt gtatttgcta gccaaggtac tttttcccca atttgattct
    12541 aatatagctt gtcctaaaat gatactagga agcactccat aaccatcttg tagttcttgg
    12601 gcatgcggaa caagccgttc aataaattgc tgatgggatt gcgcttcatt ttcttgctga
    12661 ttatcagtaa aaggagaaga cagattcttc aaggaaaaaa caaaggctaa gagtatcact
    12721 gctacaccgg caaccactgc tggccaacgg atattcttgc gcttttgctt tttccacttt
    12781 tttttcgcca tgatcaattc ctacttttca tttaattcat tcagatattc ttctagcgtc
    12841 aattggtgtt tggtgatata ctcagcgttt tctttaccga cataacgtaa gtgccaaggt
    12901 tcatatgtga tcttagttat atcttcgtgt ccttttggat aacgaacgat aaatccatac
    12961 tgcgcagcat tatctgcgat ccatttagct ccaggctgat ctccgtaact tgcatccaat
    13021 acagtagaag gataactgtt gtaccagttt tgatcgacga catcgactgc cagacctgtg
    13081 tggtgttcgc tgtatcctgg ctccgtaatc gtttctttag taatctttgt cgcctcctct
    13141 tctgatacac cttgacgact catcacatct tggacatttt gttcgaaaac ttgttgttgg
    13201 gatgatacag accggtaagc tgagaccatc actaatggaa atccagctgc ttcagcagcc
    13261 ttggcaaatg cttcatattc tggagcaatg cgtttgtcga tcaagtatcc gttacttaat
    13321 gatgccaatt gagtagactc atcaatttct ttttctaatt tgtgatctgg accaaccaaa
    13381 actagcttcc aatcgtctgt tttaacatct ggcaattgtt tggacgtacg atcttttgtt
    13441 tctttgtcct ttaccttgct gctggttgtt tgactgttct tttctttact agcagttgtt
    13501 ttggtgcttg ttcgttcgac tgcttgattg ccggcttgaa catgcgaatc atcatcgcta
    13561 aaaataacat agcccattgc tgcgatcata atcacaactg ctctaaatcc taaaaattta
    13621 ttcacttgaa aaatcctttc gttgttcttc acgttcccca ttcgcctcat gaacgtaagt
    13681 ttcttccaga ttttcattca cccaatcgag aagttcgatt ttttctcctt cgatctgttt
    13741 ttgaacatct gctggcagac gaaaattcaa aatatcatca tagccccatt catcatattg
    13801 aatacttact ttcaagtcaa ggtatctacc agcctgctga ttatttttca aagggatcag
    13861 ctcgactttt gctgctttgc tgctaagcca tttttgcgcg atgcgtgttt tgatttgctt
    13921 gtactctgca acggcaacgt ttcgtttttc gggaaaaata atcgtttgac gcaaaatctt
    13981 ttgcgttagc atccattcat aatcagtgaa aagatttttt actttctgca tcgcttcaga
    14041 tggcagattt ttctcgccat ctttccattt ttgccattgt tctggactga tcagcaagta
    14101 ttcatctaaa aaattttttt cagtttcaaa ctgttctaat aatacactca taataagatc
    14161 aatatatact tgatgcacgt ttatcccctc cttttaacta ttctacttaa gttttgatca
    14221 aaaagcacct cttaaatcgc tgctttcatt ttttttaatg tttcaagtcc tctcctcttt
    14281 attcttcgtg atttttgtta taataataga cagacaagaa actcaatcca actatgaagg
    14341 agcgttttca gatgaaaaaa tatcaatggg gaatcgttgg tcttggtacc atcgcacatg
    14401 agtttgccga aagcttcaat caagagacga gcgaactaac agcagttgct tccagaactt
    14461 cagaaaaagc ggaaaacttc gctcaccgtt acaatattcc aaaagcttac ggaagctatc
    14521 aagaaatgtt agacgatgct gaaatcgaca tcgtgtacat tgctgtacca aatagacagc
    14581 atatcgacca tatcttggca gcattggaag caggcaaaca cgttctatgt gaaaaagcca
    14641 tcacgatgaa taaaaaagaa ctggctgacg ctatgcgctt agcagaagaa aagaatttga
    14701 tcttagcaga agcgatgaca atcttcaaca tgccacttta tcagcagtta cgctcaataa
    14761 tggatacagg aaaactgggg gctttaaaaa tgatccaagc acctttcggc agttacaaag
    14821 aaccagaccc taaaaaccgg ttcttcaatc cagaattagc tggaggagct ctgttggata
    14881 tcggtacgta tgctgtatct ttcgctcgtt tctttttgag ctctcaacct gaagtagttg
    14941 cctctacaat ggttcctttt gaaacaggcg tggacgaaca atccgtcact atcttgcgta
    15001 acaaagaaaa tgaacttgct gctgtcagct tgacttttca ggcaaaaatg ccaaaagtcg
    15061 gtatcgttgc ctttgagaac ggttatgtga cgattgctga ttacccgcgt gctgatcgtg
    15121 cggagatctt atttacagat ggtacaaagg aattcatcga aagcgggaat acatctgaag
    15181 caatgaatta cgaaataaag aatatggtac acatgattga aggtgacttg cccaatcgct
    15241 ctctcttctt gacaaaagat gtcatcgaaa tcttggatca aatgcaagtt ctatggaaaa
    15301 atatgtaatt ttaaaaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    15361 acaaaaatag ctcctcctat ttttcgtttg gctggactta ttaccgcaga ggaaagcttt
    15421 tgaacaccgt ttaagaaaga ggttgtgagc ctaccgttca gctccgagtc acaaggagca
    15481 atttcaaaaa acagcttctc atgtttgttg aatattgtga cttgtgaccg aggagttgac
    15541 aggagaacac cgtttaaaga agaggttgtg aaatcagctt tttgacctga atattagctg
    15601 aattcacaac ctctattttt ggattttttg ttgggagaat tctgccttga tttcaataaa
    15661 acttttaaaa gactgcaaaa actggaacaa ttctgctcga tcctcgaatt cagggcgact
    15721 agtcggcaga ggcatttgcc gatacaatac ataaacttct tcaatacgtg ttaaaatttg
    15781 tctgccatca tttgactctg aaaatgtttc tgcagtataa atcaataaac catagatatg
    15841 ttttccataa taagctggtt gctggattct ctctagattt tccagcatgt ctttaatcac
    15901 attggcctgt gctcttctca tagagaagta agtttcgtag tatagcggct gtctgagcca
    15961 gtagttttct tgatgttctc tggcatcttc ttggctttcg cgaatgaatg tcagtaaatg
    16021 ttcgcatttg atctgcacat cttctttatt ttctgataaa agaaattctg ccatttgtct
    16081 caaaatattt cgaaattctt tttcgatctg tatttggttt tccttcagct gcttcgtacg
    16141 atctggcatg taaacatttg caagcaaagc aaatactaat ccaatcgcca ttaatcccgc
    16201 ttcattgccg atcagctgcc aagacatgtt ttcctccact aaataatgag tgaccaaaac
    16261 cgaatttact actatccctt cggttaattt gaagcgagag gcaagcggga tgaacaggaa
    16321 taaaaaaaga ctgaagccaa aaatagtata cccaacagca aaaaaactga acagtgctgt
    16381 tgccgtagct aatataaagg ctgcgattcg attcatgccc gtacgcaatg tagagcgctt
    16441 ggtatttcct acacttaata atgcaatgat ccctgcagct ggccaataca acagcgataa
    16501 gctacccgct gtgaccatcg aaaagaagac actgatcact gttttgactg ttcgcattcc
    16561 tatacgcatt tacttccccc ttttttattt taattatact tgaataatga aaaactagct
    16621 atcagacaaa agtttaggta tgatagtaaa aagaccaaaa aggagaatct ctatgcaatt
    16681 tggccgtttt cgactaggta tgcgtacgat caaaagtgcc ttagctgttt ttttatgtat
    16741 cttgtttttt catttgactc aacgaggaat cccgatgatt gctgcattat ctgctgtttt
    16801 ttctttgcgt caggacttga ctacaaccgt ttcttttgga aagtctagga tcatcggcaa
    16861 tacattaggt ggtacattag ctatcattta tttttttgtc aaaagctatt ttcacaatga
    16921 ttttttggta gaattgatct tgcttcctat tctagttatt attgtgatcg tcgtgtcaga
    16981 tggtattaac aataattccg gtatcatttc cgcaatagct acgttgttgc tgattgcgtt
    17041 gagcatccct caaggcgaat ccgctttgta tgctatccaa cgtgtactag atacttttat
    17101 agggacattc attgcaatcg gtatcaattt ctttcttcgt cctcctgaaa cagaaaagaa
    17161 ggaagaacta tcagaagatt tggtcgaact ccaaaagaaa gaacgattgc taagaacaaa
    17221 tttacaagag atagaagcta agatccaaaa acaaaagaaa aatagcgagg actaattttc
    17281 ttttgcaata aaataaaaaa atggagagaa gaactttaaa aagtcttcct ccatttttga
    17341 ttgaacggtg aagtgtacta ctttattctt ccttcatcga gttttgttct gcctcttttg
    17401 gcgcttcata catatttact tggctagtag tcccacgtgt cgacaaattc tctacgattt
    17461 ccttcaagtc aagtcctgtt gtcgctttta gtgtttcttg agtagaagaa agcaaatttg
    17521 tggcataatt tgtcactcgg ttcgctccac tggtttctcc tgaactgctt cctgtatcga
    17581 caacagagat tttttcgata tttcctagcg gctgtgctgc ttcgcgcatc aattgcggta
    17641 gcatatcgat aaccatactc aagacagctg cttcgccata ttccttgaag gcattggcga
    17701 tcttttgttt tgcttcggct tctgcttttc cttttgctag tgctgcttcg gcttctgctt
    17761 gtccagctaa acgtgtctta ttggcttccg cttctgccag tgcttccaca cggaatcgtt
    17821 ctgcttcggc ttcagtcact tctttgactt tttgagcttg tgcttcttgt tctttagcgt
    17881 accgatcggc atctgctttt ttcttcactt ccgaatcata ttgcttttca cgacgtgtga
    17941 tttctttttc ttccagttcg atttgttttt gacgttctac gattttaatc tgcatttcct
    18001 gttcgattac ttgttgttgt gctcgggcac tttctaaatt gtaggcttgg tctgcttttg
    18061 ctttagcaac atcttgttct tgtttgtagg ttgctaattt cagttctttt tcttttaatg
    18121 attctgcgat ctctgtttgt cgctgtaatt cagctgcttg tgattctttt tctgcttgag
    18181 cttttttgat acgcgtttct tttagagctt ccgcttctgc tatatctgca tctcttttga
    18241 cttgggcgat tcttggtttc cctaaagagt caagataacc gtttttgtct ctgacctctt
    18301 tgatagtgaa agaaacgatg atcaacccca tttttgcaag gtctacagaa gcgacttctt
    18361 gtacgctttg actgaactta tcgcgatttt gatagatttc ttctacagtc atagaaccaa
    18421 gaatcgagcg caagtgtcct tctaatactt ctcttgcttc attttccagt tcttctcttg
    18481 tcttgcctaa aaactgttct gctgctgtag caatctcttc tacagatgaa ccgattttga
    18541 tgatcgaagt accatcacac atcaccggta cgccttgttc cgtatacact tcaggggtgg
    18601 agacgtccaa tttactcgat aatagactga tccgattcga acgttgaaaa actggcaaga
    18661 cgaatgcgcc acctccacgc acaattttga ttttgttatt gctttcatca atgtgcacat
    18721 ttttgcttcc taaataactt ccactgatga tcaacgcttc atccggtttt gctgtctggt
    18781 acttcgctac aaagacgatc agcaacatca gtaaaatcaa agctgcgatt gcgatcgcga
    18841 taaaaacaac tggtatttcc atttaacaca cgctccttta agcttttatc atgctttcat
    18901 aaggcatgac ataacagatt ctttccttga tctcaatgat caatactttc gttccccgct
    18961 cagctgtctg ttcttggggg ctgtaaaaac aagctggacg actaatggaa ccggtaacac
    19021 ttttcaactg aatttctccc atacctcgta cagggatcgg tgtgatcaca acagctactt
    19081 gtccttccag agttttttca gaagacgaca tcgttgattc ggcatgtttc atcggcatca
    19141 atatatagaa attaagcaaa aataccaaca ctatcgatat gcctccacta gcaataaata
    19201 aaaataaaga tgattgatct gttaaacgct cacctagaaa accaaataag cttgtgaaag
    19261 ttaaccaagg gataataagc atcggatcaa gcgggccatc aaaatcgaat acatctccaa
    19321 aaataatcaa taggactgcc aatcctgcac aaatcagcaa tgtataccaa taaatcgtct
    19381 caagtgcgat tccccctatc atatacacgc ctccttccat ttctacgtct atcataccaa
    19441 tgtttgaaaa ttctgtatag cttcttttac cgactcagtg gtgtttttta taggaaggtt
    19501 gtgtttatcg ttgacaattt gttaaatttt tgttttcatg ttaaaatttt cacaataaat
    19561 tctttcgagg tagcctatgt ttcctttttt taagaaaaaa ccattaaagc tccagaacac
    19621 gatcatcctt gtcgtttgtt tctccacatt cgtttctttg attgtttctg ccatattgat
    19681 caggaatttt attctttcga tgaaccgatc tagtgtgtca gataaaatct ctagcattgc
    19741 taaaatcgtt gccaatgata aactcatcca aaaaaagctg cagactggtg acccgacaca
    19801 tgcgattcaa ggaataacaa atgaaatcat gattgcatcg aatttagatt ttatcgttgt
    19861 catggaccct gaaggcattc gatattctca cgcagaccca agttacatcg gtcttccttt
    19921 ctcaagtcat gaagatgtga aaaaagtgat tggaggacaa gaatttttct ctgaaaaaga
    19981 aggaatgtta gggttcggct tccgctattt cgtccctatt tattatgatc aagagattat
    20041 tggtgcagtt tgtgttggct taacaaacaa cacgattgac aatcaagtca aagatggaca
    20101 aaaaatgatt gttttttcat tgattattgg acttagcgta ggcatcatag gcgccgtttt
    20161 tcttgcccaa aaaatgaaaa aagtactgct tggacttgaa ccacaagaaa tagcagaaca
    20221 gttaagtgaa aaaacgatta tcgaaaatga agtagatgaa ggaatcttag ccatctcttc
    20281 tgataaaact atcctcttga tcaatacacc tgcaaaaaac attttctacc aaacagagca
    20341 gctattggat ttcgaagtag gaaaaaaatc agtgataatc tatatgacgt tttgtttaaa
    20401 gagacgattg agcatcaaac gaccgttcgg actcaatcca tgtatttaaa tgggatagaa
    20461 actatttcta gcgttacacc tctttttagc aatgaccgtt ttttaggagc agtcgcaact
    20521 ttacgaaatc gatcagaagt aatgaaatta atggaggagc ttggggggac gaaacaatat
    20581 ataaactcca tgagagctca aacccatgaa ttcatgaata aacttcatgt catttctggt
    20641 ttgattgatt tgaagaagta tgaagaagtc acccatttta tccagcagct aaatcaaaat
    20701 tataaaaaag agattggcca agtctcaagt ctgatcaaag tacctgcaat tgccggtttt
    20761 ttattaggca aaataaacga agccaaagaa caatctgttg aaatgctgtt agaaagcgat
    20821 tcttttatcc ctaaaatgga gatggatgac tccatccatt cacttttgca agtgttaggg
    20881 aacttacttg ataatgcaaa agaagccgtg ctacaaaaac atgaaagaaa cggacaggtc
    20941 cggattcatt taaactatga cacagaagct gaagtattca tcgtgaccgt ctgcgatgac
    21001 ggtgtcggga tatcagaaga aatctcagaa aaaatattta cgctaggttt ttcgacgaag
    21061 ggaatcaatc gtgggtttgg attgaatctc atccattcaa tcgtcaaacg ccacggtgga
    21121 tttttagaat tttccaaaca aacaaatggc ggtacaatcg tctatctaga attaccaaaa
    21181 aaggaagatc aaaatgatga caaatgtcct gataatcgaa gatgatccaa tggttgcaga
    21241 attgaatcgt acttttgtgg aaagcatccc taatatgaac gtaatcgaaa gtttcagaaa
    21301 tgtagaagaa ggaaaaaaat atttcagaca aaaaaacgca gatctgctgc tgcttgatgt
    21361 atacatgcct ggcgaaagtg gtatccattt tttatcttgg cttagacagc aaggaaatga
    21421 tatcccagtg attttgatca ctgctgcaga tgatatcgaa accataaaga atgtgatccg
    21481 ctacggtgcc ttagattatt tgatcaaacc gttcactttg caacgttttc agctggcgat
    21541 cgaaaagtat cttgctttct ctcagacaat ccaaacgact gaaaaagccg atcagaaatt
    21601 gatcgatcaa gtattcaacc acaccactcc cattcacgaa ataccatcat tcatcgattt
    21661 gcctaaaggt ctgtcaaaac tgactttaaa aaaaataagt gcatttattc taaaagaaga
    21721 tactattttt tcaacaaaag aactggctca aaaaagctca ctgtcacgta tatccgtcaa
    21781 gaaatatttg aattttttag tagaacaaga tatattgaac gaaagaatca aatatttgga
    21841 aatcggccgg ccgctaacct gttatgaatt gacaaagaat gggcggaaaa agctcacaga
    21901 ttactgttga gtcatcgaat tatcccatac aaaatagtcc aagtatacag aagtaacaac
    21961 tgtttacttg gactattttt aaacatttta ttgacgaata tccatgtttt cttatcttaa
    22021 ttgacgggct ttaggaagag atacctcatt tttcgttttt ccagtttcta ttatttcacg
    22081 aaagttctcg aaacttgtcg aaatcatatt tgataatgcc tcatctgtat ttgaacctac
    22141 atgcggggtt acaagaactc tagggtacat ttctactaat tgctggatag cagggtctgg
    22201 aatcgtctcc catggcttga aggatcggaa aaaaacctct gtttcatttg caaaaacatc
    22261 ggttgcaaaa ccagctaaat gattgctttt caaagctctt aaaatagctt cattatcttg
    22321 aagctctcca cgggctgtat taatcaagat gctacctttt ttcatttgag ctaaaaatcc
    22381 ttcattgatc atttgatcgt tttcacctgg gatatgaggt acatgaatac tgacaatatc
    22441 gcttttggct aataactctt caagcgagtg gaacgtcaat acttttttag cagcttctgt
    22501 ctgatgaata tcgtagccga tcactttggc tcctagtccc ttaaacaaaa cagcttccgt
    22561 caaccctatc ctgcctgttc ctatgatccc tactgtgcaa tttctgattt cacgactaaa
    22621 catttgctca tccactcgaa aatctttgaa agatgttcgg atcgtcgtat acgcagtatt
    22681 tctcaaaagc atcatggcaa aagtcaaaga taactctgct atcgcatttg gcgaatacga
    22741 tggtacacgg gctactgtca taccaaaatc agctgccgct tgtagatcga tatgattgac
    22801 tcccacagtc cgtgtaaaaa cataacgaat cccatattcc tgcatttttg caagattggt
    22861 ctcatctgct atacaatttc ccctcaacaa tacagcatca tggtcctttg ctgacacgat
    22921 attgtcatgt gtcaacagtt cttcataaag actgagatca taatggtaaa tattcaattt
    22981 ttcaaaaaaa tctatctcgt tcggacgaac accgtaacat gcgattctca tactttcttc
    23041 ctccatttct tatgaaaata aacctatgcc atgaatcatt tgtaatacaa cgatccatac
    23101 aggtgtcagt accacagcaa aaatagttga cagtaaggat gcattggagg ctaaaagtgc
    23161 ctccctatca aaactgatag cataggctgc tgccactgtt gcagctggtg tagccatcat
    23221 gataacgatt gttgccagtg cttcaaaatt gactggtaga atattcgttg ccgtcaaaac
    23281 tgctagaatc acgatattca atgcaggtac aaataccact ttgactagac tgtaatacca
    23341 tgaagttttt tcagatgcag cttttttgaa gcttacttca cctaatgttg cgccgattgc
    23401 tagccacgcc aatggtgaac ttaaaccagc taaataatcc atcggctgaa atagccacgg
    23461 tgcagtctgg tcaattcgca aaaacgctac ttggcttatc tctccttctg cacctgtaac
    23521 tgacacttgt ggcagatacc cttggaacag ccaaatgaaa aggccggcaa aagtagcgat
    23581 aatgattgga ttgacgaaca tcgctttgat atttttagct tccatcttca agccactcat
    23641 ttttatgtat ccataagaat acaaaaagat tcggtagcca atattgaaaa ttgaggaata
    23701 catcacgccg acaggaccat aaatcgcact gacgataggc gttccgaaaa aggtcgtaga
    23761 accaaagatc gtcaggacac gcaacgttgt ttgcttatcc cctttatagc ttaagaaaaa
    23821 aggttttgaa actagaataa gaataatata aataactaat ccccaaatca agacattgat
    23881 tccttgcatc agtaattctg tattgatatc ttgcatgaaa gctcgaaatg ccagtgctgg
    23941 caaagccact gttaagacga ctttcgatag aatttttcca acagattcag aaaaaatccc
    24001 cttttttcta caaacaaacc ctaataatat aataaaaact gttgaagtga ttgcactgac
    24061 aatcgacata tctgtcaaag tcgatcgaaa aatgtcaaat aagttcatct ttctcctcct
    24121 aattatgttc atttattcac aataaaaaaa gcttttattt tgctaatacg tattaacatt
    24181 tttaatttta tctttacatt gttacttatt gaacaatagg aaattaaaga aacttttgaa
    24241 acttatgtaa agtgattttt
//
LOCUS       DAZBSA010000038        23827 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000038 DAZBSA010000000
VERSION     DAZBSA010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.038"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(53..787)
                     /locus_tag="ACHGMT_001392"
     CDS             complement(53..787)
                     /locus_tag="ACHGMT_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFN6835142.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="ACHGMT_001393"
     CDS             1114..1524
                     /locus_tag="ACHGMT_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFN6835143.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="ACHGMT_001394"
     CDS             1517..2218
                     /locus_tag="ACHGMT_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFN6835144.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="ACHGMT_001395"
     CDS             2218..3804
                     /locus_tag="ACHGMT_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6835145.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="ACHGMT_001396"
     CDS             3823..4044
                     /locus_tag="ACHGMT_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFN6835146.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="ACHGMT_001397"
     CDS             4041..4802
                     /locus_tag="ACHGMT_001397"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFN6835147.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="ACHGMT_001398"
     CDS             5182..5691
                     /locus_tag="ACHGMT_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFN6835148.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="ACHGMT_001399"
     CDS             complement(5766..6305)
                     /locus_tag="ACHGMT_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFN6835149.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="ACHGMT_001400"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="ACHGMT_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFN6835150.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7318..8169
                     /locus_tag="ACHGMT_001401"
     CDS             7318..8169
                     /locus_tag="ACHGMT_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFN6835151.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8197..8832
                     /locus_tag="ACHGMT_001402"
     CDS             8197..8832
                     /locus_tag="ACHGMT_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFN6835152.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="ACHGMT_001403"
     CDS             8852..9625
                     /locus_tag="ACHGMT_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN6835153.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="ACHGMT_001404"
     CDS             10033..10830
                     /locus_tag="ACHGMT_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFN6835154.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="ACHGMT_001405"
     CDS             10808..11221
                     /locus_tag="ACHGMT_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFN6835155.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="ACHGMT_001406"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="ACHGMT_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFN6835156.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="ACHGMT_001407"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="ACHGMT_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFN6835157.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /locus_tag="ACHGMT_001408"
     CDS             15998..16558
                     /locus_tag="ACHGMT_001408"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFN6835158.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="ACHGMT_001409"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="ACHGMT_001409"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFN6835159.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="ACHGMT_001410"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="ACHGMT_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFN6835160.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="ACHGMT_001411"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="ACHGMT_001411"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFN6835161.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGVFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="ACHGMT_001412"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="ACHGMT_001412"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFN6835162.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="ACHGMT_001413"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="ACHGMT_001413"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFN6835163.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="ACHGMT_001414"
     CDS             21905..22483
                     /locus_tag="ACHGMT_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN6835164.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="ACHGMT_001415"
     CDS             22535..23017
                     /locus_tag="ACHGMT_001415"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFN6835165.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="ACHGMT_001416"
     CDS             23134..23508
                     /locus_tag="ACHGMT_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835166.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(23621..>23827)
                     /locus_tag="ACHGMT_001417"
     CDS             complement(23621..>23827)
                     /locus_tag="ACHGMT_001417"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835167.1"
                     /translation="NLTTFENFRHKIFTTNDYYISIPTNNPNNTFSFLTPYPASSPAS
                     RTFFYQKNLYHPIDSTNQPNAVML"
BASE COUNT         7780 a         4286 c         5115 g         6646 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaca
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcaccgggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggtat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaacaa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagtt
//
LOCUS       DAZBSA010000039        23484 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000039 DAZBSA010000000
VERSION     DAZBSA010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.039"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..572)
                     /locus_tag="ACHGMT_001418"
     CDS             complement(27..572)
                     /locus_tag="ACHGMT_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFN6835168.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(584..1276)
                     /locus_tag="ACHGMT_001419"
     CDS             complement(584..1276)
                     /locus_tag="ACHGMT_001419"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFN6835169.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1295..1579)
                     /gene="macP"
                     /locus_tag="ACHGMT_001420"
     CDS             complement(1295..1579)
                     /gene="macP"
                     /locus_tag="ACHGMT_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFN6835170.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1592..2155)
                     /locus_tag="ACHGMT_001421"
     CDS             complement(1592..2155)
                     /locus_tag="ACHGMT_001421"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFN6835171.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2383..3045
                     /locus_tag="ACHGMT_001422"
     CDS             2383..3045
                     /locus_tag="ACHGMT_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFN6835172.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3169..4365
                     /locus_tag="ACHGMT_001423"
     CDS             3169..4365
                     /locus_tag="ACHGMT_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFN6835173.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4466..5842)
                     /locus_tag="ACHGMT_001424"
     CDS             complement(4466..5842)
                     /locus_tag="ACHGMT_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN6835174.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5879..6746)
                     /locus_tag="ACHGMT_001425"
                     /pseudo
     CDS             complement(5879..6746)
                     /locus_tag="ACHGMT_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
     gene            complement(6835..7860)
                     /locus_tag="ACHGMT_001426"
     CDS             complement(6835..7860)
                     /locus_tag="ACHGMT_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN6835175.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7878..8552)
                     /locus_tag="ACHGMT_001427"
     CDS             complement(7878..8552)
                     /locus_tag="ACHGMT_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN6835176.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8736..9629)
                     /locus_tag="ACHGMT_001428"
     CDS             complement(8736..9629)
                     /locus_tag="ACHGMT_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFN6835177.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9703..11400)
                     /locus_tag="ACHGMT_001429"
     CDS             complement(9703..11400)
                     /locus_tag="ACHGMT_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFN6835178.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11393..12706)
                     /locus_tag="ACHGMT_001430"
     CDS             complement(11393..12706)
                     /locus_tag="ACHGMT_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFN6835179.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12706..16647)
                     /locus_tag="ACHGMT_001431"
     CDS             complement(12706..16647)
                     /locus_tag="ACHGMT_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFN6835180.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16756..17214)
                     /locus_tag="ACHGMT_001432"
     CDS             complement(16756..17214)
                     /locus_tag="ACHGMT_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFN6835181.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17512..18030)
                     /locus_tag="ACHGMT_001433"
     CDS             complement(17512..18030)
                     /locus_tag="ACHGMT_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFN6835182.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(18032..18649)
                     /locus_tag="ACHGMT_001434"
     CDS             complement(18032..18649)
                     /locus_tag="ACHGMT_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFN6835183.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18646..19500)
                     /locus_tag="ACHGMT_001435"
     CDS             complement(18646..19500)
                     /locus_tag="ACHGMT_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6835184.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(19497..20408)
                     /locus_tag="ACHGMT_001436"
     CDS             complement(19497..20408)
                     /locus_tag="ACHGMT_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6835185.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20449..21783)
                     /locus_tag="ACHGMT_001437"
     CDS             complement(20449..21783)
                     /locus_tag="ACHGMT_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN6835186.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            21991..22821
                     /locus_tag="ACHGMT_001438"
     CDS             21991..22821
                     /locus_tag="ACHGMT_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFN6835187.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVDGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(23065..23314)
                     /locus_tag="ACHGMT_001439"
                     /pseudo
     CDS             complement(23065..23314)
                     /locus_tag="ACHGMT_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         6859 a         5127 c         4104 g         7394 t
ORIGIN      
        1 ttattttatc aaatcacttt gtttttttat aaaatttctg cgcctagtgt ggactcaaaa
       61 tgtctcaatg cccattcatg tccaacggga tcgaaacttg ccacggaatc tttgaaaata
      121 tggagtttgt atcctagatt atacgcatcg actgctgtat gcaacacaca gatatctgta
      181 catacgccgg tcaaataaat atctgtgatc tggcgttctc gcaaacggat gtctagatct
      241 gttccactaa aagcggaata atgccgtttg tctatccaat aaacattttc ctcttgctca
      301 tagtcttggt ataaactttt taaagagccg tacaattccc gtccacccgt tcctatcaca
      361 ttatgcggag gaaataagcg gttctccgga tgaaaggaat cttttggatc atgcgcatca
      421 atggcaaaaa cgacaaaatc cttttgatca ataaacttct ttgtatgagc agtcagcttt
      481 ttttcaatta cttgtcctgc tgtcccagta gtcagctttc catcctctgc aacaaaatca
      541 tatgtgtagt caatcgagat caatgcgttc atcttagtcc tccttatacg agatgtttga
      601 caaattcgat caccatttct gctgatcgtt tgcctgcgtc ttcgataaat tcgtcaaagc
      661 tttgtgtcgc cgagtgatct gctgtgtcgc tcattgcacg tacgatcaaa aatgggatat
      721 tgaactgacg agctgtttgt ccaacagcgg ctccttccat ttcgcaagct aatgcttcag
      781 ggaaattcgt taagatttct ttgattttat ctggcgagtc gacaaatgta tcacctgtaa
      841 cgatcaatcc ttcttttgcg ttcaaattcg ttgcttttgc tgctttaacc atttcactgc
      901 gcaaatatgt gcttgcttca taatatagag gcattcctgg taattgtcct gggttgtagc
      961 caaatcctgt tgcatctgca tcaaaataag ctactttgtc tgatataacg atgtcaccta
     1021 cttgtaagcc ttcaccgatt ccgccagctg aacctgtatt gatcaccatg ttcacgccgt
     1081 attgttggat caacagactt gtggtaatag atgctaagac ttttcctata ccggaacgta
     1141 cgacgatcac ttcgtggtta cctaatgaac ctgaaataaa taaagcgcct gcgcgttccc
     1201 atgataatgg ttcgcttagg ttctctctca aaatttttac ttcttcttcc attgcgccga
     1261 taataccgat tttcatcttt ttaattcctt ctttctatat aaatgcgatt gctaaaaaaa
     1321 ccacacataa cattaggata acaataataa gagatttcat caaaaaagag ttccatttgg
     1381 tcgtcttttc ccgttcgctg atccttgttt tcgtgattgg tttattcttt ttgctttctt
     1441 tacgataaaa gctggcaatc tttttttctt cttgctgata agctgtttct gcttttcgct
     1501 gtttttttaa tgactcgctt gcttgtgctt gttgtcgctt gcgaagttcg cttcgcgtaa
     1561 taagcggtcc tttggccata agccgatccc tctactttcc tttggtcaat agttcccagt
     1621 attgaatcgc ataaatcgtc tttgcatcac aaatcaactg atctttcatc gcttgttgcg
     1681 cctcttcaag agttagatga tacaactcca gtacttcgtc ttcatcttgt gctaaaggat
     1741 tctcgacctt ctctacccct tgtgcatgat aaatatgaag gacttcattt gcaaaacctg
     1801 gtgaaagata catcgaagta agatggctca gcgacttcgc acgatagcca gtttcttctt
     1861 ctaattctct agctgctgtc atttcgggat tttgtccttc tcctggatcg atttttccag
     1921 ctggaatctc caaaatcact ttttctaaag gttttcgaaa ttgtttgaca agaagcagac
     1981 gatcttgttc gtcaaaagca atgatcccga caccgccagg atgaaatacc agttctctct
     2041 tcgaagttcc tccatctggc aaacgaactt cgtctacagc aacatcaata atttttccgc
     2101 tatagatttc tttgcgactg attgtttttt cttcaaattg ctcattgttc agcatatctt
     2161 ctcctgcttt ctttgtaatc ataactgctt ttttttcatt tttcaatttt tctcaaaaac
     2221 tccttattag tttaccatag ataaacctta gattttctct aaatatagaa aatccttctt
     2281 tagtccgata ctctttctag tagtccgtat gctaaaataa gtatgtaatc gaataacgaa
     2341 aagaattcaa tactcaacga taaatgacat ggagggttta ttatgataaa acgaaattgg
     2401 ccgtggcttc tagcattggt cttgctattt ctctttgtcc gaaacaatag tttttctttt
     2461 gttctaggac ttttattgat aggtgtagtt ttagttttgt ggggactctt tggttcgcgc
     2521 aggaaaaagg gaaaacaaga agaaattcct gcactgactg acgaacttga aactcactat
     2581 gaaaaaagcg gtatgacagc gaatgaaatt tcattttttc gacaaacaat gaatcaaaca
     2641 aaaaatgaga tcaatcaatt gcaaatgaat atgcagcaga cagccaaact taaagcaatc
     2701 gatctccgcc atgatccagt caaagcagcg aaagctttat tcaaagaatt ggtgaaagaa
     2761 ccaaaccgac tgcatgaagc aagtcagttt ctttatacgc atctgccgaa tctagttgat
     2821 ttgacaaata agtataatga gatcaatgac catgagatca aaaacaaaca gacctatgaa
     2881 aaactagaag aaagcgcgca gatcatcgac cagctgtcag ctttaatcgc acaagattat
     2941 caaaaattcg ttgctgagga tctagatgat atcgacgtag agatatctgt tgcgaaacag
     3001 agcttaaagc gtgataataa atatgaacca gaacaaaagg attaactctt ttgttcataa
     3061 aagaagactc ggaacattca gatgatgtct ggctgtttcg agtacttttt ctaaagtaga
     3121 gacattcatg taagaatcga gaacagattc aacaaggagg aattttttat ggatcataaa
     3181 ccgactgaaa caaaagatgt tacaccagtt aacgatacac ttgaagattt attgaacaac
     3241 ccgttttcaa ctccaacaga tacgctgacc acttcacaac aagcggagat tgatcagctc
     3301 caagagcaac agacagctgc tcgtttaatt gacaaactac cggcggaacg ccaagaacaa
     3361 gctaaacagc tagcagcaaa aatcgatgct aatgatgcac agtctgtcat cagctacgga
     3421 tcggctgccc aagcaaaatt aagtgaattc tcacaatcga tgttgaatca tgtgcaagca
     3481 caagatattg gtcctgtagg agattcctta actgaactga tgtatcgtct ccaagaagcc
     3541 aatcctgacg aactgcgtgc cggagaaggg aacatcttcc agcgtgtctt cgggaaggtc
     3601 aaacaatcga tctatgaagt gacagcaaaa tatcaaaaga ttggtgcaca aattgacaag
     3661 atttcagtga aattagataa agaaaaagac ggtctgctaa aagataatct gatgttagaa
     3721 cagctttacc agaaaaacaa agactacttt gatgcactga atatttatat cgctgctgga
     3781 gaattgaaaa tggaagaact tcaaacaacg atcattccag aagcgatgaa aagagcagaa
     3841 gaaactggcg atcagatgga tgttcagatt gccaatgatt acacgcaatt cctagatcgt
     3901 ctggataaac gtacgcatga tcttcgctta gctagacaga tcacgatcca gcaagcacca
     3961 caaatccgtt tgatccaaaa cacaaatcag gccttggctg aaaagatcca agcatctatc
     4021 gctacagcca ttccgctgtg gaaaaaccaa gtcgtgatcg cattgacctt gcttcgtcaa
     4081 aaagatgcag taactgctca acgccaagtc tctgaaacaa caaacgactt attgaagaaa
     4141 aattcagaaa tgctgaaaat ctcagccatc gaaacagcga aagaaaatga acgtggaatc
     4201 gtggatatcg agacccttca aaccactcaa aatgatttga tcgaaacaat ccaagagaca
     4261 ttgcgtatcc aaaaagaagg aaaagaaaaa cgtcgtcatg cagaagtaga gcttggacac
     4321 atggaagaag atctgaaaca aaaactgttg gatttaaccc aatagatcac ctttcgcttg
     4381 ttactaaaac aaacgcccaa caacagttta ttacaactgt tgatgggcgt ttttcttatc
     4441 tttcttctaa cttctccatt ggagtctatt tctcattcat cttgctgtgt ccatagccat
     4501 agccaaaata aatgatcagt ccaataacca ttacgatacc gaacacgatc catgtcacag
     4561 tttgcagacg aagcatcaat acaatacaag tcaaaatgga gacgattggc aatagcggaa
     4621 cgaatggcac tttgaattct ccagcttttg gtttgccaaa catttttctc aacttcagta
     4681 agcctagact aacaagcatc aaataggcga gcgccatgat attcgttagt tccgctaata
     4741 cattcaatgg gaaaaatccg gcaaagaata cagctgccac accagcggca taagtcgctt
     4801 ttttcggtgt acgatgcttt tgatcgattt ctgtcataaa cttaggaagt aagccatcct
     4861 tgctgacagc aaacagtaaa cgagcaagtg aatacatcat cgagatcgtt accgtcagta
     4921 gcgttaaaat tgcccctaca gcgatcaccg cgcccactgt tccatgtcct acatacctca
     4981 ttgcaaaagc cactgggtca ttgactccta gttcagtata cggaacgatt cctgtcaaga
     5041 tcagcgtcac gatgatgtaa aggacagtag caatcagtat cgaaccaatg attcctcttg
     5101 gcacatcttt ttgcgggttt ttgacttcct cggctgccat tgcaacggca tcaaatccca
     5161 agaaagcaaa aaacacaaga gctgcaccat ctagcacacc ggaaaaacca aaaggcatga
     5221 atggctgcca gttatcaggt ttaacgaaaa atattcctac aatgataaac aacgcgatga
     5281 taccaaattt gacatatacc atggcattat tcaaacgtaa tgctttcttg gcttcaagcg
     5341 acacgatgta agtgacgaat atcactacga gcaccgcaat caagtcaatg tatgtaccat
     5401 tttccggatt gtacccgcca gttaatgctt gtggaagttc ggcccctaaa cttttcaaaa
     5461 aaccttgcat atagccagac catccggaag caacagagga aaccgctagt aaaaattccc
     5521 cgatcaacaa ccaccctgtc agccaggcag caaattcacc aaatacaaca tacagatagg
     5581 catatggccc accaatcact ggcactcgcg aagcaaattc tgcgaaactt agtccagaca
     5641 aaatgacaac gattgcagct aagacaaatg aaagtgacag tgctggacca gcattttgat
     5701 tggccgcaac ccctgtcacc acaaatattc ccgtaccgat gatcgcacca attccaagca
     5761 tgatcaaatc agacgttttt agttcttttt tcatctcact cgtatgattg agattgatct
     5821 ctttcttacg aaacaaatcc attgaagttc ctccccattt taggttggaa aacagcgatt
     5881 aaactgtttt catataccct aaaagggata ccacctaaaa tgactaaaag gcaaccaaca
     5941 ataacataaa tgagctcttt tttcgttttt ctttcgccaa ggatgaaaat cccacctaaa
     6001 gtagagataa tgatccccat ttgtgaaagt gagaagccaa cagctaaacc aagtgatttg
     6061 actgttagaa gcatgcagat gtttcctaag ccccataata atccacagat catgtttttc
     6121 cacacaaact gatcgacttt aactttacgg aaagcaaaga agcttgcgcc taagatcatt
     6181 ccgatacttt gaggtaagat aattgacatc gcatccaaat tcgcccagtt gacgatgatg
     6241 gtatatacac cataaccaat cgtagagaag atcaatgctc taaaaccctt accaaaatca
     6301 agcattttat tatctgtatc tatttttcct tcgtcatctt gtcgggcagt caaatacgca
     6361 ccgcttacaa gaagagctaa agcaataata ccgtatacat aatctttcgt ttgtgtccat
     6421 tcatggaaga aaacggctcc cgcaatcgta ttcaaaatta attgaaaacc agtagacaat
     6481 ggtagcccta cagaaacacc catgtacttc attccatgga actgtccatt ttgtccaaca
     6541 ctccaagcta agccggataa aaatccgatc aagaaaatcc aaggggtgat tacaggacta
     6601 acaacgaaaa agacaactaa agaaaacaga aatgcaccga tcgtcattcc tagtgtttgt
     6661 tgattggcat cgccaccaat tttaccactt actaaaccaa tactacccca tgccaacata
     6721 gggactaatg ctattaaaat ttccataacc aatctccttt tatttcacat gataagaaac
     6781 ttatcagcga aaacgttaac aacacaagca ctttttttcg tttctaacga ttttctaacg
     6841 tttttcatta taaaaattta agattgggaa aaataaagtc attgttgtcc cttttccttc
     6901 agttgattca gccgtcaaat ggatacctaa tttttcggcc agattttttg ctaaataaag
     6961 tcctaatcct gttgaatgtg ttttgctaag tcgaccattt ctcccagtaa atcccttatc
     7021 aaagatccgt ttcaagtctt ccgctgggat accgatccct gaatctttaa ctgaaaggct
     7081 gactccttgc gcttcctttt ctatgctcac aatgatgttt ccgtgatccg gcgtatattt
     7141 gatggcatta ctaagcaatt ggttgaagat aaaagcgatc catttagcat cggtcaaaac
     7201 agtatgatcc tcaccaacga tctcgtagcg aatatttttt tggataaaat aatttgcctg
     7261 actgcgtatc acaggctgaa tgattccttt tagtccatat tcttggatga ggtagtcccg
     7321 cgagaaactg tcgagtcttg cataatacaa aacttgttct acgtattcat cgatttttga
     7381 taattcattt tctagtaatg tatacttttg atcgtcaata tcgaactcga tcgaacgaac
     7441 tagcaattta gaagcggaaa gtggcacttt gatttcatgg acccatgagt caatataatc
     7501 tttttggtct tgttgattac tgattgctga ttgcattact tcttggtgtt cacggatcag
     7561 ctgattgata tattcttcta ccagtttttc ttcttctgtc ctcccaccac tcaaataatt
     7621 ttgcaaaggg gaagtttttt gtatgtcggc taattttccc caccatcttc tcttgctcaa
     7681 gtaaccaccc agcagataaa aaatcagaaa gacgccctca atcaaagcta aataagcaat
     7741 tgtgctcaaa tcaactttga tgtttggcgc cagccacatc acaaagatcg tcaatgcaat
     7801 aaaaaacagc catcccaaga tcaatgtcca ctgatctttc aaatatttaa aaaaattcat
     7861 cttaaccgct catcctttca gggaacgata tatccctgtc ctacttttgt ttcgatatat
     7921 ccttggatac ctgcctgttc gatttttcgg cgtaaccgat tgatgttgac tgtcaatgta
     7981 ttgtcatcta caaatcgttc gtcatcccac agtgctcgca gcaatttttc ccgacttaag
     8041 atctttccat tgtgcttcat caaaataaat aatagacgat attcattttt gctcaaatca
     8101 atgatctctc cgttgatttc catacttccg ttgtcaacat tcaacgtaat gccattgtgg
     8161 ctcatcattt cactagataa ttctgtgtag ttgtaagaac ggcgaagtaa tgcattgatt
     8221 tttgcaatca gtacatcgat ttggaaatgt tttgtcacaa aatcatctgc ccccatgttc
     8281 atagccatga tcatatccat attcgtattc ctgctggaga taaaaatgat cggtactttc
     8341 gatacttcac gtattttttg acaccaataa tagccatcca aaacgggaag attaatatct
     8401 agtaatacaa gctgtggttc ttctctttca aagtctgaga acacttgatt gaaatcggtc
     8461 gttccaaagg tttcaaattg ccatttctca agttcctcca tgatcaattg tcgaatgatc
     8521 tcttcgtctt cgactaccat gatttttgcc ataaaaaatc cctccatctt tcttcttgct
     8581 actacaatat caaagaagcc cttgtgaaac aagttggctt acctggaaaa tgtcatttta
     8641 gcaaacgtta aaaaaaactg gaaatatgcg atttaatcgc gtttgtctct tccaagcagc
     8701 ttaaatttat tatgttattc ttaataattg gtctattatc tgttattttt ctttcgttgt
     8761 ttttgtcttt taaattttcg ataacaccaa taaccgaaaa gcgttagcac tagcaatata
     8821 cctagaagga gtataagtaa tcgataaaga tggtattttt ttgtctgttc tatctttgaa
     8881 gagacttctt ctgtaaaggg aactcgtctt cctgtcacta gtaatcggtg agtgttcacg
     8941 gtgtaaggtg tacaagtgat aagtgtgact agatcctgct tttctctaat tgctaatgta
     9001 tttgtttttg ttggaaggac aacaattttc tcaacgactt cataagcgag catccgacca
     9061 tacacttcaa gatagaatcg gtcatcttgc tccatttctt caagatccgt aaagagtttt
     9121 ttctcagcaa taccgctatg ggcggtaatc acggaatgtg tactctctcc tccagttgga
     9181 aaagaggtgc cttgcaggac tgtcgctcct ctatctaaca aaagatcatt cgtttcatca
     9241 aataaaggca agctaacatt gattttcgga atatagatgg ctccgatcat atgttgttcg
     9301 taatattctt ttcctggatt ttggacattt ctaaccgctt gctcaaaagg atctttgact
     9361 tgtcccattc ctgggattac tgatgtatga gcaagcgtct gatttttctt ttcatgcact
     9421 gctaattgtt gctcatgttt ttcttcttta tcgcgttcaa gctgcttttg ataattctcg
     9481 atccttcttt gatccaaata attgctgaga gcatctgcta aaaatggata tgtaaaaact
     9541 gcagctccgg caagaaacat cactgccata aataattgta ataaaagact gatttgggtt
     9601 tgaggtctat tacctatttt ctttttcatt ttcttcacct tcttatttaa aaaaactgca
     9661 gcaaccttta taaaaccgat tgctgcagct tatttttgtt gcttatactt cagacttcat
     9721 tttagattta cggtaccagc tgtacgctcc aatcatcaaa cttattccga ttagtaagaa
     9781 tgccaagagc ccgttcccac cagtagcagg aagtgacccg cgtttcatat tcggtactct
     9841 ttctggccct ggattttcta ctcctacgat ctgaatttct tcattatagc ttccataaga
     9901 gatgataaat tcagtaaagg ctgcctcacc tgttggaagt acataacctt ctggcgccat
     9961 ggtctctttc atttgatagg ttccataatc taacccttga acctcaaagt atccatttcc
    10021 atctgcgaca aattctgttg cttgtccgct tgttgcccac tcaattctat ctgctcctga
    10081 ttcataaaca gctaaaggcg actccccttt ataaaatatt gcgtattcag aacggtcttc
    10141 attccacaat tcgaatcttg catctttaag caattcactg cttgatgcat catatttttc
    10201 aaactttctt ccacctgttg tgactttctc tggcggctct actccttctt cataatcaaa
    10261 ttcatcattt cgtccgattt caataaccgc tgtgttgtta tggaaatcat ccgcttctag
    10321 ttctgtggaa accttcaaat tatacgtgat cgttaaatct cctccacgtg catctgctaa
    10381 tctacctaaa gtatctactt tggctactcc attttcttct gtcaaaatcg tcacgacaaa
    10441 cccgttgctt actacttcta ctatataatc ttgtcctttt gttaaaatcg tctcgccggc
    10501 tcgcacttct atttcctgat cataataagt tagaccaact gtaggcgtat ctgtgatttt
    10561 gtaatttttg ataacagcgc ttccgttttc aaggacagaa tcgatgaaat aaggaatagg
    10621 tgctgtgatc gtgtaagaag ccgtttttcc tctttctaga tcaatataag aaatcgtttc
    10681 ttgttctcca gcttcattaa taatcgttac ttgtctaagg tcgctttcct ctacattcat
    10741 ctgtttatct gcttctctga tttcgttttt cggatacaaa tgcacatctg tattgtatgt
    10801 gtctttccat gtagaaccat cccacacgcc atccaccata tcaggcatca tcactggcat
    10861 cataacaacc attggagacg cgattctgga aatatgagct ggcgaataag tttctaagaa
    10921 aagatagact ttatctctag aagaaggtgc ttcttccgtc acttggacgg tagagaatac
    10981 cgcttctcca gcttcattcg ttgtttgttt gtcaatactt gtcagtcgat attttaaaaa
    11041 ccaactttct gtgtgccgtt tttgaatcca ttcaatcgct tcggcagaat taagaccgtc
    11101 ttctcgttcc ggtgttaaag gattatctcg aatcaaatcg taatatactt ccgttacgtc
    11161 aaacaaatca aaatcgactc ctggaatatt ttcttcacca aaatccgtag aaaccaatcc
    11221 gctattcggt cgttcttccg gaacagatga gaaacctttt ttgtgtaatg taatcgtcac
    11281 ttcacttggt ttttgtgcga cttcttcggc tttgactagt tttccgttcc cgaaaatccc
    11341 tattcccgca gtaacgagta acagtacact cattcccgtg acaatcattt ttttattcat
    11401 ttttttctcc ccgttctttt tttattaata aaacagcacc taacaataaa agaattccta
    11461 gtaaagaaat caacgttctt gcttcattcg tttgcgggaa tacggaccat tttcctgatt
    11521 gacctggatt gcttggacca ctcggctctt tcggtttgtc cgttgtttct tttacggtct
    11581 cgttataaac acgtggtgtt gcggattcat aggcagagac tggaactttc ccggattcta
    11641 attcagccat ctcctgtccg ttaactacta catattgtgg ttggcctttt gcatccaccc
    11701 aaaattcagg gatgatgacc tcgattctct tacttgcttc atccatctcg taaccttcca
    11761 caccttttaa ttcttcaaaa taataggtgc cagaaggcaa gaaacgttgt cctgtcgtaa
    11821 caaggccctc tttatccgaa atgaatttgc ttacattttt gtcattcaaa ggatcattgt
    11881 ctgctggttt gatccattga tttttcaagt cattagcagg agccatatct aaataatatt
    11941 tccccccttc tatcatctga tatagagcaa aaacagcacc ttctaatggt tttccttttt
    12001 cagttgttcc tctttgacgt ccatacttga agaagtatgg atctcttaga taaccgacat
    12061 ttttaggata gatatgaatc tcttgcagtt cctcggttga ttctgtcggg tgataaattg
    12121 gcaaaatagc agcaattggg atagctgtct gatcgatatc aacatccaat ccaacttctg
    12181 gatcaatcga ttcttcgaaa atcaaataaa ctgcatcccg atcttggttt ttcttaggtg
    12241 ctttgatacg tgcaatcccg tctcctaatc cgccaaccgt atctttccca gttgttaccg
    12301 aagtccctat ttggacaagc ctttcttctg tgattagctg acggatattg tttctgttcg
    12361 tttcagcgat ttgctggacg aattctttag gagtcatgtt ttcagcagct tttttttcat
    12421 gataataatc ggtcatgtcg taaatggcaa atgtcgctcc atttagtggc accgtctgtg
    12481 aaatgatatc tgctgtttcc cctgcttcct cagagatagc caagccatca ttttcatggt
    12541 attcaaattg atcgttataa tcgatatctc gaatcatccg tttatgcaac acgaattcca
    12601 cgacatcttc ccctgattct tctgccgaca tcgcatgtgg aagaaggact gctgcaagcg
    12661 gtagtaaaac aagtaattga agcatcaaac acaacagctt tttcattacc ttgctccctc
    12721 ctttttagat tgtctgacaa acaaaccata ggctgctcct gcaagcgcca tgaagaatgc
    12781 agcaagcagg atatagaata atcgtccgga tcctcctgta gcaggcaacg gattttcagg
    12841 atcgttcccg acactaagct gtaatctgat tcgatttgtc tccaaaagtt catattctaa
    12901 ctcttgctgt agatcaggcg tctctgcttc ttcgtttgga taatacgaaa tctcaacttt
    12961 tagtgtaccc gcttcttttc ctggaactac ccgatcattt tctatgtcag gttcttctcg
    13021 ataactaatt tctaaaataa agtaacctgg aagttttcta tagctgtcag gtgacttcac
    13081 ttccttgatc cgatagaccc cttccaacaa gtctgaagca ttgaataggg aagaatcgtt
    13141 tggatctggt gccaagcctc cctcgatgat ttcatactct cctgcactat tttgtttctc
    13201 aattgaaaat tctgcgcctt caagacgtcg gctattcata ctgtcaattt tcaaaagttc
    13261 caaatcgatt ggcgctagtt catcttctaa ggttacttgt agtacaccat cgatgatttc
    13321 atagccttcc ggcaagggag cagtttcgtc ttcgatgacc gtatcttcaa tgataaactt
    13381 accttcttca ttcagtgcaa actggaacac ttgatcagat agccgatacc catttggtgc
    13441 gacagattcc gagacctcat attcaccaat agacagatgc agaggttggt cgtaatttaa
    13501 atcgacatac ttttctactc ctacggcacg agaatagaag tatccgatcc caggcaaacg
    13561 tttcgattct tctggttcta acggattacc agtgtatgct cgatacagcg ggaaggaaga
    13621 atcccccact ttcctgatat cgaaacctgc accatctaat gtatggcctt catggtctaa
    13681 ttttctaact cggatctccc gataatcaaa cgtattgtaa gcagtgaatg ctacccataa
    13741 acgatcctct tcatctgttg tatacgtgag atattgccaa ttttcttcac ctgtttgctt
    13801 gagttggata gtccctatag cattatcgta aagtcgcaag atccatgtgt cattttcatc
    13861 aggttctttg aaaccttctg ggttctggct ctcaatgatt ttgtattctg catagttggt
    13921 agtttcttca ctatccttat aaacaaatga atctggctct ccgattggat gaataacttt
    13981 ctcaccatcc acgattacat atttcccgtc ttcatctact gaatagaacg tgagtctccc
    14041 tttctcttcc tctacttcgt tcacaccaga aagccctttc gctactggat atccgatttc
    14101 cagtccgtta ccatctgttt cataaagttc aaactctgcg ccattgattg gcatattact
    14161 gccttcaatt agtttattga tattcatgtg gattggcttg aattcatttc gcatttccag
    14221 tttgacatac aactcatcat actctgtgcc ttctatgtta ggctcttgta caacagaaag
    14281 cttgtgatat tctccttcat aataggaatt cggatcttta ccttccggtc cgccgactct
    14341 cgaaccagat gtatattcat ctacaagctc gtagtgacct aacatctcat cttcgttggc
    14401 tgcatatttt ttgatttgga attgatacgt ctcatctact aactgataac gtggtggtcc
    14461 agcgatctct ctgatccaaa atatgtcgct gactgctcca gtttcatctc ctaaaatcgg
    14521 catcttcgtt tcaaagatcg ctattccgtc cgtggtagtt ttacttgttt cgattggttc
    14581 ggcttcccca ctctctggat tttggtcata taatgcaaat tcgactccag ccaacggctc
    14641 gccattttga cccttcttgc tgacttggaa attacggcct ttatgttcgt aacgaatgga
    14701 agaagatggg aaatacatcc gtccagtagt gatatcattc tcataatcct ccaggttctg
    14761 aatataggcc atattgttca ctgtgcaaaa atttccgtct tgagctcccg catcaagttt
    14821 tacttggtac ttgatcctga ccatttcccc ttcacctaaa gaaatcggag aaaatttata
    14881 agcttggttt tcccgtccga catacgttgt cggagtcgcc actacttctg gtggggcttg
    14941 tccatgacgt tcttcatccc atatttctgg ctcttggtac ttcatgccgt tttctcctgg
    15001 atctagatgg taaaactgaa tatctgccga tctgccagct ccaaatggtg agacattaaa
    15061 accagtttca tcataaagtg tggcttgctg gatcgttctc ttgaaggtag aagagatatc
    15121 ctccaaggct gtaacaatgt cattcttgtt gttcgcattg taatatcgcg gtgttacgcc
    15181 atcttcttct aaatcagcaa tatttttcaa tacatttcgt ccaatagcat ctagtcgttg
    15241 tctcccgcta acgtttccat cgattcctaa accaatggag aagatacttg gcgggttctc
    15301 ttgatacttg tttttcagcc aatgatgata agcaactgtg ttgatcgcag atgacttttc
    15361 ttgcgaagta ggactgccat tcgtattgtt attagaagaa ccatcaccaa cccagtgagt
    15421 cgctttatta tctccttctt ttacctctcc ataacgatag tgtacattta cttcatcttc
    15481 tggacggtta aagtcagatg cataggtaac tggatgagta aaccctgcat tagaagtatt
    15541 cccagaaaac tcctcaaaat ttctgaacaa aacgtcatta tcttcagcaa tcttatcttc
    15601 catcacagtc cagttgctcc aagctccatt agtgctcgta cgataacttg actgaatcgg
    15661 tgcataactg aaggtcggtg tcccatctcc cacaacgatt aggatttttt ctggatttct
    15721 attttctccg ccattgtctt tatacaatgt ttcatagcca tttttaagcc ctagtgtaag
    15781 cggagttcct ccgattggtg tacgatttaa tgctgtattc tcacttcccg tcaaacgatt
    15841 gatatcattt gtcaatggaa agatgtccga actgatttgc tcatggttat tgatagctgt
    15901 actattatgg taaaaattca ccataccgat tcgaatatcc agactcgggt cagagagcag
    15961 atctttactc atttcgttta ctgcctcaat caacgcagca tttttagttt gactattggt
    16021 cccagcggtt aattcactca tactcgctga cttatctaga acaaaaacga tatcgattgg
    16081 actgatttcc tttttctcgc ccccgatgac atctagatag acattgtaaa gggttgttgc
    16141 atcttcagga tgtagatcag atgctgtgct gcttggcaga acaggttcta cccatttttt
    16201 gatgtacgcc ccgttataat ccaagtaact gttttcaaaa ttccgttcat ttccccaaag
    16261 ctgaacggtt tgagctggaa cgtttctatc ttcttcgcct gcagttgatc cattattagt
    16321 agcatagtcg taatttcgcc agttcgctcc cccgtttgta taatcactat catcgatcaa
    16381 gttctctccg tcatatggcg agcctttgtc tccttgatag cgttcattgc tttgtgtcca
    16441 atttcctaac acttttgtat agtcattcgt tgtaaacttc gggtagcgat gctgttcgtt
    16501 ttcaacttct ccgctaggat agatatattc ataggggtct tgcgtagctg ttggcccaag
    16561 acgctccaaa ataccgttga ataactggcg ttgaatgcgc tggagtgctt gaccgctggg
    16621 tacgattggt tcgtccagct tttccatctc attttcttcg ctacgttgaa tttcagcgga
    16681 atcttctgta tcagtttcat tttcttgtaa ttcttggtta tctattcttt catcaaccac
    16741 cacttgaaga ggtagttacc cttcgttcac atcggggatc ttttctgttt caaaaatcaa
    16801 tgtgccggtt tctgcttctt ttgaataaac ttttccgaca taccattctt gaccatcgat
    16861 cgtctcgact tttaggtctt ctccatcttc atcataggag gatagacctg ttccacgaac
    16921 gttttttacg gtaccaatcc cggcggctgc cgcatcgatt gctaaacgta ttctaccttc
    16981 attggcactt tccttttttt caaaagccag atgccattca agggtttctt ctttttcaat
    17041 atacttccct tcaatttttg catagtcctc atctaagaat acctcatcct cctttgtttc
    17101 tgttgactca gcagctacat ccagggtcgt catatgatag actgactgga aatatggtcc
    17161 aaatagcaaa ctgacagtta ctaactgcaa aagcttcaat tttaaccctt tcacatcctt
    17221 atactccttt caaaaatatt tgtcttcttt cacaacaatc aacaaaacaa tgcccatcta
    17281 attataattt aatttaattt taattttaaa tcaaatgata tcctttttag ttaaaaaaca
    17341 ccaattatac aacatttatt attaaaatta aattataaaa ttttataacg ttctcattta
    17401 acaaaactta agttattaag acaaatgaat aaacaactac gctttcctcg aaacaaaaaa
    17461 ataaagcaca aatgcgcatt tacgcactca tgctttattc gtacaattaa ttcagataac
    17521 ggcagcgcat aggatgccaa taacacttcc taaaatagct cctcctaaaa tgtcagtagg
    17581 ataatgtaaa agtaaaaaaa tcctcgaata agcgatcaaa caagcaaata aaaaggctaa
    17641 tattcctgca actggactta attcgaacaa cgcagctact gataaaaagg ctagcgctgt
    17701 atgtcctgaa ggaaaagaag aagtcttcgg tttacttgtg taaaggacaa gccgacgatt
    17761 tttttcataa ggtcttttcc gtcttgtcgt ttttttgata aaaagcaaac caacaaaaaa
    17821 atagatcaaa ctgccgataa gaatggctag accatattct ctagtatcag gtatcaagac
    17881 taaaacagca gcaaggagca accaaataaa accaccatta gagatattac tgataaattt
    17941 caaaaaattg atttttcgac tatccgtttt ccgttgcttg gcgaacttgt tgataatact
    18001 atcatcgatt ttagcaatca aacgcaacat gttattcctc ctttttaaga accttttctt
    18061 gcgtacgatg gataaaaagc aggacttgaa aataaaacaa gaaactgaaa cctaataaag
    18121 caaaatagat tggtcctgca aaccagaatg cgacaagaag gatcaacgtg cctgccaaat
    18181 agaaaagcgc gcttttgaaa tcattaaaaa acaagaaaaa tgcagtaatc caagaagctt
    18241 ctttttgttc cagttgaagc aagattttgt aactaaagag aagcgcgctt aatagaagca
    18301 aaaacaacgt agtatacatc aatgtatagc caattccccc accccaatag cggaaaatct
    18361 gataatcgat caatagaaga agaaaataaa cacttgctaa actatcttgc tttccataaa
    18421 gagagaagtt ctcacgaaac tttttccaga atagtgaacc gagctttttc tcttcatttt
    18481 ttttgtaatg ataaatcata tacagaactg ttcgacttgc agttcctaat gtgaaaacag
    18541 gcaagacgca caggaaccat agtagctgta aatggataag acgtggaatc cattcaatga
    18601 tttctaagaa ataatcattg gcttttttac caatcgatcg attcatcatc cttttgaacc
    18661 tcctgtcaaa tccatccctt caatgaaata acgttgtgca aaaaagaaca gcaaaatcgt
    18721 cgggatcaag acaagtgttg ctcctgccat cagataattc cattgtgtcg ttgcttggtt
    18781 ctgaaaaact tgcaagccaa tctgtaacgt gtatttttct tgatcttgga tatataacag
    18841 aggacctaag aaatcattcc aagctgcgtt gaaggaattg atcgcaatgg taatcaaggc
    18901 aggtttcgtc aatggaagca tcaaatgact ccaaatatac aaatgattcg ctccgtcgat
    18961 ttcagcagct tcgatcaatt cattattgat cgataagtaa aattgacgca tcagaaatat
    19021 attgaacgca ttgccgaaga acgatggaac gatcaggggc aaataagtac ctacccagcc
    19081 gatcttggag aataatacat attgagggat catcgttaca aaccccggga tcatcatcgt
    19141 agaaagaacc aaagcaaaca tcagcttttt cccaggaaaa tcaagcttcg caaatccata
    19201 agcaataaaa gaatttgaaa ggacattacc gatcactact agcacagtga tgaataatgt
    19261 atttcttgca tagcgccaaa aaggaaaggc ggcaatcgta tccagatagt tttcccatac
    19321 gattttttct gggaaaaagg tcggaggata acgaacgatt tcttccattg gtttcaatga
    19381 agtaaacacc atccaaagca atggaatgat caaaatgatt cctattactg tcatgaggac
    19441 ataacttagt attttttttg tttgccgatt cacactggtc tttttgaaat aatccatcat
    19501 ttgccgtcac ctccagaata atagacccac ttaggtgcaa gcttcaaatt gacaaacgtc
    19561 agaaccaaca cgatcagaaa caggatcatt gacatagcca tcgcataacc catatcaaaa
    19621 gctacgaacg ccttgttcca catatgcaga ttataaaaaa gcaaagaatt tgcaggtccg
    19681 cctgttccat ttttcgtcat gacataagct tcttgaaaaa tttggaaaga accgatcgta
    19741 gaggtgatca catcgtaaaa aataatagga gtgattaatg gcaaagtaat acttttgaac
    19801 tttccccaaa cgcctgctcc atcgatttct gctgattcat ataaagatct aggaacattt
    19861 tgtaatgtag ctaagtaaag tagcattgct cctccagaac tccatatttt catcaaaatc
    19921 aacgcaggtt tcgtccattg tggatcaaac agccaagctg gtccagggat atggaaccag
    19981 cccagaaatg tgttgatcaa tcctgcagat ggagaaagca gctgcatcca taagatgtag
    20041 actgctactc ctgataagac tgccggtaaa tagaaagctg tacggaatac actgattcct
    20101 ttgatttttt gattgagtac tgtggctaaa aacacactga aaatcgtcgt cagcggtaca
    20161 ttgaagatga cgaaataaac ggtgttgtat aacgatttcc aaaataaatc atcgtgaacg
    20221 aacattcttt tgaaatttgc tagaccaatg aaattcatct tggatgtcaa attataatcg
    20281 gtgaaactac caataactga aaaaagcata ggccctagca taaataccaa gaacccgata
    20341 ataaaaggcg agataaataa ataggcccat cttgcttctt ttttctttcg gatagataaa
    20401 cgcttcaaat tttcttgcct cctctgtacc tagacagtca aaaatggatt attgattttg
    20461 cttgactaag ttttctactg ctttttgtgc atctgccaat ccttcttttg gtgattttgt
    20521 tcctaaaaga atctggtcaa tctgttcatt cactaatgtc gtgtaatccg gagcatctaa
    20581 aggaactggt gtgatcaccg tatttttaaa gttttcgtcc gccatttgat agatcatttt
    20641 tcctttatcc gttaggttgt cattattcac taaattctca ttggctgtct tgctcgccat
    20701 gatatcaaaa cttttttcgc cgaatttttc ttgtacttct ggagaagaaa gatatttgat
    20761 gaattcataa gaggcctcag gatcttttgc tccgtatggt acttccaaga cgaatccgcc
    20821 accccacgac caatggcctg aaccttcttc tttttctggg agctgcatca cgccaaaatc
    20881 aaaatcatcc ggtgcatttt ccatcaaact agagtaataa ttgatatttt gggcacgcat
    20941 ggctactaat ccggaaataa atggatcaga tacaccagac ccaaattcgg cttctaattt
    21001 attgatcgta tttttcccat aataatcttg ccagtcttgg atccactcca aggcttccac
    21061 tttattttta gtatcaatct tgactttttc atctttgtca aaccaactag tcccgttatc
    21121 tgcgtttagc gcccatacat cagcccctaa attccataat ggataaaaac cgatgcgtgt
    21181 aaattctttt ccttcttttt tatctagttt acgtgctgtt tcttctaatt catcccaagt
    21241 tgtcggtaga tcttgttcac tgattccagc ttctttaaat aactttttat tataaaaaat
    21301 gacttgtgta tctgtattga atgggatcgc ataaggatca ccttcatatt ctactgtatc
    21361 ccaaagctgc ggataaaaat ccttagaagt gtctttttct acatatttgg agagattagt
    21421 tgcttgctgt gcttctgctc tttgggcaac ggaatttatg tcctgtacaa taacatcggg
    21481 tgcatttcca gctgttattg cagataaaga tttagtccag atgtcacccc atggctgata
    21541 cttataattt acatggatct cgttttgtga ttgattgaaa tcctcaatga tttcttcgat
    21601 agcttctttt cgtgcaccgg atccccaaaa ggaccagaaa tcaatatttt ttcttccatc
    21661 acttgttttt tcagaagaat ttgctgcact tgaactggca tcgcttgcat tacttgatcc
    21721 gcatcctcct aataaaaaca atgctgaact tgccagcaac cctgtaaaca ataatttttt
    21781 catattttac ctccaaattt taatgtaacc gatgccggtt ttaatataac gatatcattt
    21841 tgttctgaaa ataacaattt ataaggtatg attctaaaaa aattattaat gataccaata
    21901 ctttatataa ttcgcgagcg ctttctttga attttttaat ctttcgtagc agaatgagaa
    21961 tgtattaaaa atagattagg aggaattttg atgtttagac accagaaaga attgcagttt
    22021 gaagcaaaac cagatcgtcc gaatccatta atcgcgaaat atctgcagga gctgatcggc
    22081 ggccaatatg gcgagatgag tgtagctatg cagtacctgt tccaaggctg gagctgccga
    22141 ggagaagaaa aatataaaga tatgctgatg gacattgcaa cagaagaaat cggccacgtg
    22201 gaaatgttgg cgacaatggt tgctcgttta ttagagggtg cgcctgttga agatcaagaa
    22261 gcagcgatgc aaaaagatcc aacaatcggt gccattatct ctggaatgaa tccacagcac
    22321 gcgatcgtag atggcttagg acctcgtcct gctgacagcg tgggcaatcc atggagcggt
    22381 gcctatatca ttgcgagtgg caacttgtta gcagacttta gagctaattt aaatgccgaa
    22441 tcacaaggaa gacttcaagt agcacgtatt tacgaaatga tcgatgatcg tggcgtaaaa
    22501 gatatgctgt ctgttctcct tgcaagagat agctatcatc aaaatctatg ggaatgtgca
    22561 gtgaaagaat tagaagaaaa agaaggttca atcttagtcc caagtacttt cccgcgtgac
    22621 aaagaacgaa aagacattgc ttatgacttc tataacttct cagagggaga ccaaagtagt
    22681 gaaggttctt gggctagtgg caaagcactg gatggcgaag gcgaatatca atggttgaaa
    22741 gaacctaaga tcgaaggcgc tgcaccaaaa ttagaaccag taacaccaaa aatgtatggg
    22801 acacctccat tcaagaaata aatatctacc cgtagaaaca cccgtttttt gttccacacg
    22861 ctttgtgagc aaaactgact aaagtcatta atcataacca cccttcaaaa gggtggtttt
    22921 ctctagagct ataagctcaa atcaccggcc agcgcctaaa gacgctggct ttcatatttt
    22981 atgttcaagc catattgcct ttgccagttt tgcccacctc ttaaaaggtt tatgtactac
    23041 tccttagccc ttaaaaggat cgtcttactc tcgtacactc agcttatcta acgcactatc
    23101 atgtttttct tgttcttgaa tgtacttttt gatggtcgct tcattttgtc ctacttttgt
    23161 cacatagtat ccttccaccc aaaagtgtcg atacccaaat ttatatttta gatttgcaca
    23221 atctcttgat gatctcctga atattggctc gatattgatt ataaattatt tttcttctat
    23281 actttggtgt gaatacaatg tggtatttac acatctactt tgtacgggct aaactatttc
    23341 cctttattgc tccacccgca tagcaagtgg ttttgtattt caatcgctcg gcgcgctcaa
    23401 ttaggtctaa agacaataaa tctaaaaacc cgctgcttca ctgacagaaa caacgggttt
    23461 tcagaaaaaa atgaaatgaa aaag
//
LOCUS       DAZBSA010000040        22678 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000040 DAZBSA010000000
VERSION     DAZBSA010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.040"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            59..3337
                     /locus_tag="ACHGMT_001440"
     CDS             59..3337
                     /locus_tag="ACHGMT_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFN6835188.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3878..5785)
                     /locus_tag="ACHGMT_001441"
     CDS             complement(3878..5785)
                     /locus_tag="ACHGMT_001441"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFN6835189.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5915..6790
                     /locus_tag="ACHGMT_001442"
     CDS             5915..6790
                     /locus_tag="ACHGMT_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFN6835190.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6870..7844
                     /locus_tag="ACHGMT_001443"
     CDS             6870..7844
                     /locus_tag="ACHGMT_001443"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFN6835191.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8024..9169
                     /locus_tag="ACHGMT_001444"
     CDS             8024..9169
                     /locus_tag="ACHGMT_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFN6835192.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9306..9650
                     /locus_tag="ACHGMT_001445"
     CDS             9306..9650
                     /locus_tag="ACHGMT_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFN6835193.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9921..11207
                     /gene="efmA"
                     /locus_tag="ACHGMT_001446"
     CDS             9921..11207
                     /gene="efmA"
                     /locus_tag="ACHGMT_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFN6835194.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11345..11944)
                     /locus_tag="ACHGMT_001447"
     CDS             complement(11345..11944)
                     /locus_tag="ACHGMT_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFN6835195.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12348..13469
                     /gene="mnmA"
                     /locus_tag="ACHGMT_001448"
     CDS             12348..13469
                     /gene="mnmA"
                     /locus_tag="ACHGMT_001448"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFN6835196.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13715..15136
                     /gene="arcD"
                     /locus_tag="ACHGMT_001449"
     CDS             13715..15136
                     /gene="arcD"
                     /locus_tag="ACHGMT_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFN6835197.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15150..16268
                     /locus_tag="ACHGMT_001450"
     CDS             15150..16268
                     /locus_tag="ACHGMT_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFN6835198.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16328..17620)
                     /locus_tag="ACHGMT_001451"
     CDS             complement(16328..17620)
                     /locus_tag="ACHGMT_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFN6835199.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17770..18330
                     /locus_tag="ACHGMT_001452"
     CDS             17770..18330
                     /locus_tag="ACHGMT_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN6835200.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18459..18836
                     /locus_tag="ACHGMT_001453"
     CDS             18459..18836
                     /locus_tag="ACHGMT_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFN6835201.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18769..20841
                     /locus_tag="ACHGMT_001454"
     CDS             18769..20841
                     /locus_tag="ACHGMT_001454"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFN6835202.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21052..21999)
                     /locus_tag="ACHGMT_001455"
     CDS             complement(21052..21999)
                     /locus_tag="ACHGMT_001455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN6835203.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22245..>22678
                     /locus_tag="ACHGMT_001456"
     CDS             22245..>22678
                     /locus_tag="ACHGMT_001456"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFN6835204.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQ"
BASE COUNT         7343 a         4142 c         4812 g         6381 t
ORIGIN      
        1 aataaattag gtaatacttt aagtaaacgc tttctaattt gttacgggag gaatacactt
       61 gttaaaaaaa cgtataaaat acagtgcgct aatgatgaat ctccttatgc tcagtacccc
      121 ggttttaatt tctgtcgttg aagttgctgc taacgaacaa cagtctgtga acgaagaaaa
      181 tgcacacgtg aacattgaag ggatgtcaac aatggaggaa ataccaagaa ctagtgaaga
      241 tatggatacg gaagaaggaa atgatcctat gatgaaagaa tcagaaaatg aaaagacaaa
      301 aaagcaatct gaagaagcaa atgaagagtg gaaaggggtt gttttcggag aaagcacaag
      361 tgctagtagg tatgccattg aaccattaga agacggcgtg cggctctctt ccacgaataa
      421 cggcggaaaa ttccagtcaa gcggatcaga tggaatgacc tattattatc aagcaatccc
      481 caaagacatc aactttagga tgcgggcgac gatcgaaata gaatcttgga cctatacgaa
      541 tgcccaagaa ggatttgctt taatggtaag agacgcagta ccaaaaaatc atttgtttgg
      601 gcaggcattt tattcaaatt catttgccat actaggcagt cggatcgaat atgtctggga
      661 ctcggataga caagaagtgg tcaacacgag cggcagcaaa tatacgatgc gacttggatt
      721 ggggacacgg gcaatcacag gaatatcatc tgaagatccg caagctgcgc cagctcctgg
      781 gagtgtctca gtggaaacaa cacctttaga gacttcagca gggttcttgg aaaaagaaac
      841 aggttcttat aatatattcg gaaacggtca tgggaatctg tttccggcca cgaactcgat
      901 tactaaactg gacgtagaga tgaaaaaaac gaatactggt ttagaggcat attattacga
      961 tcctgaaaca ggagaagaaa tggcttctga catcatgtat gactgggaaa aattatacgc
     1021 ttcagatgaa tcggttattt atgcaggatt tgctgcagca aggaatatga cgatcaaggt
     1081 agaaggaatc gaagttgctt actcagatcc tgcgacagat cctcctgggc aagaaagacc
     1141 tactcgcttg attgatccga tttacactgt gacctctagt aatcattcag gaaaccaaga
     1201 tcatactatg tcctttagag caaatgctga cggattattg acaattaaaa ataaagcaac
     1261 aggagaggtg cttaagaacg caaaaaactt ggctatcact gcgaatcggg aatttgatta
     1321 tcagacaaaa ctgatggaag gaacgaacga atttactttc agttttacgc cagataaaaa
     1381 ctatccgcca gaagaatacg tagagatgac tagttatgaa acaaaagaga ttcttcatac
     1441 agtggactac cgtaaaccta aatattcgac agtttatgta acaccagaag ggtcagatgc
     1501 aggaaacgga agcagacaaa atccattatc tctgacacaa gcattgagat acgcatatgc
     1561 tgggcagaca atcgtcatgg cgcctggaac ttattccttc gaaagaggat tgacgattcc
     1621 aaaaggcgtt aacgggacaa gagaaaaccc gattcagttg attgcagaga aaaatccaga
     1681 aaacaagcgt cctgtacttg attttaaagg aactggaaat gggatgacac tgttcagtca
     1741 ctactggggt ttacgagggt ttgacattac aaatagtgca gctatgcaaa aaggattaca
     1801 gatttctgga aatcataatt tgatcgaaga gattcatgct tatcggaatg gaaatacagg
     1861 aatccaaatc agcggtagct caaatgatcc tttcgaagca tggcccgcgc ataacttagt
     1921 caagaactgt acatcttttg aaaatgccga tccaggattt gaagatgccg atggatttgc
     1981 ggccaaactg acaataggag aaggaaatgt atttgacggt tgtatcgcgt atcataatgc
     2041 agatgacggt tgggaccttt ttgctaaaaa tgaaacaggt tcgatcggaa aagtaatcat
     2101 caaaaactca gttgcttatc gaaacggctg ggtaccagga attgagggag aaggtaacgg
     2161 taatggattc aaaatgggcg gttcttcttt aacgggccct catgaattga tcaatagttt
     2221 gtctttcgaa aatcttgcga aaggaatcga ttccaatagc ggaacagata ttatcgtcaa
     2281 tagcagtaca agttttaata acggttcata taatgtggct ctttatacct cttcagcagg
     2341 aaacacagac taccatgctt ctggtatctt atctttccga acagagaatc ttgagatgag
     2401 agagcagatc cgcccattga atcaaaacga agatcaaatc taccactcgt tgaactacta
     2461 ttggaatgaa cgagagaaac aatcagaaaa tactcttgga cataccatat caaaagattg
     2521 gatcgagtct ctaaatacat gttcgaaaga aggtcagcag cctgttacgc gctatgaaaa
     2581 tggaagtatc catgttcatg gactgatgca gattaaaccg gggaatcgtc aaacagaaaa
     2641 ggaaagagtg gatggtcttc cattatctgt aggtgctatt tttgacggag aaacctcacc
     2701 gtcttcggaa tatccagagt atgcaatcgt acaagaacca aatcagacaa aaccaaatga
     2761 acaaaataat ccgattatta tacaagaagc aaatggtgtg gcggagaatg cagaaggaaa
     2821 tatcattgaa ctgaagcctt ttacagtttt tgtgtcaact ttcacaccaa atcaaggaac
     2881 gcctgtcttt gaagatatgc gttatttccc agaaaagatc caaatcgttg aaacacagtc
     2941 aagtgctcaa ttttctaatc ataccgctat tttttcagca ccttcaaaag gagaatatac
     3001 gctagaagtc acttatcgtt cggaaatatt tgatggggaa aaatggtctg ctcatacaga
     3061 atctgttacc cagcaaaaag cgatcactgt gcgagaaact gccgatgtgc ccccgggaaa
     3121 tgacaaacca gaaagagaaa atccaggaaa tgaatcacca aatggaaaac caaatggaaa
     3181 accttctcat ccggatagga cagcaaaaga aagtgaaaaa aatggcactc aaggactccc
     3241 aaaaacggga gaatttaata acccgttgct agcattagct ggaggaattt tgttaatcgg
     3301 tgtagttgtt tatgtcatga aacagcgaaa aaaataagtg gaatttctcc ttttatgatg
     3361 taaaacaatt gactgaggaa agtaaaaata gaaggaaatg agaccaatag atgtctcact
     3421 tccttctatc tttttataga tgaggttctc cttgctgctt ttgattggct gttcttatta
     3481 cttgatgcag gacagctttt tcacaatctc tccactaaac ggtgttcaat cagctgaccc
     3541 tcggtattag ctcaacaaac ggcaaaatat gaggaactat ttttccgttt ttttctctaa
     3601 tactcaggtc aggacgctga tttcacaacc tctcgataaa cggtgttcaa aagccagttt
     3661 ctcggtagta agtcaaattt atgcaaaaat gtggaaagcc attttcacaa aatttttctt
     3721 actacttgaa actaagcagc tttttcacaa cctctaatac atggtattcc agaagtaact
     3781 tcttcgaaaa ataagccgaa atgtcacaaa aatttgagaa gcgattttcc caaattcctt
     3841 cttatttctt gaagctgacc acttctggca caacctctta ccagattcgc actctttttt
     3901 ctggcgctag atacatagcg tcttgttccg tgataccaaa tgtttcatag aattcgtcaa
     3961 ggttttttgg ttgcacgttt gctcggagtt tagcaggggc atggacatcg atttgcagta
     4021 aaagttgttg gtattctttt tttgcttttg tacgccaaat cgttgcccaa ttgacaaaga
     4081 attcctcgat agaaagatct gattccgttt ttgctgcttc caatgcacag cttagaccgc
     4141 ctgcatcagc aatattttcc gaaacggtta attttccgtt tacttttccg tcagcaaaag
     4201 gcagaccatc gaattcttcg atcattgctt gtgctttgtt ttgaaattgt gtaagatctt
     4261 cttctgtcca ccagttgttc aagttgccat attcgtcaaa taaagcgccg ttattatcaa
     4321 atgcatggga aatctcatga gcgataacag ctccgattcc accataatta gcagagcttg
     4381 attgttctag actgtaaaat ggtgcttgaa ggatcgctgc cggaaagacg atcacattcc
     4441 gaaatggatg ataataagca ttgaccgtgt cagcactcat ttcccattct gaacgatcca
     4501 ctggtttgtt ccatttgctg aaacgatctt ttaatgtgag tttgctgaaa acaagagtat
     4561 ttgaaagcaa agtaccaccc tgatctgcag gtactgtttt gaactggtcg aataatgctg
     4621 ggatcttatc ggggtagcct acttggatac ctaagttatt taattttatg acggcttttt
     4681 cttttgttgc atccccaagc cagtcatttt cttctagacg ttttttataa acggcgatca
     4741 tttttttcac catttgttct acgtcttttt tagcagcttc gccaaaatat ttttttccgt
     4801 agtaatcgcc aactacttga tcgaattgtc cagaggcaag gtaatacgct gcttttttct
     4861 gaggcattgc ttcgtctgtt ccagacaata cgcgagaata gattccgctg acttggcgga
     4921 attcttctga tagataacca ctaagagaac gtactgtttt aatgatcatc cagcttttga
     4981 ataacggaaa atgagcttct gtcaggatgt cattcaaatg atcgaagtac actggatcag
     5041 ttacgatcac tttatctgga gttgtcccaa tcaaacccgt gacaagtttt ttcaagtcca
     5101 gtttatctgt gtatttaata aatgtatcaa aagattgcgg attatacatt ttgctgtaat
     5161 ctgcgttttc ttctgcactt tttacatgag gagcaatcaa tttatcaaat tgcactgctt
     5221 gttccgtgat tgcttctgcc tgactttttt ctttgccggc aagctctaat aactgaacag
     5281 ccatatcaaa gaaaacgcct attagttgtg caccgttggg atggtctgat gcataatacg
     5341 tcttatctgg aaggaataaa gaaggcggat aagcaaagaa ggcgttcgtt tgcgcattct
     5401 tcatatctgc atctacatcc agattaaatg gcaatggtaa tgtatccaac agccattctg
     5461 gaaattgctt attcaattcg tcgaaagact gcaatgattc aactttttcg attataggtt
     5521 gaagcggttg agcatgcaac tcatttcttc ggtcataatc gtttgccagt tgataatatg
     5581 caaggaaatg tttcattttt tctgaagaca ctttttctgg atgggcaagc agatcgtctg
     5641 tttctcccat caaaagttgg tcgatcccgt caactaaatc ttggaaaccg cctgtagctg
     5701 gtttatctgc agggattttt gctgtttttt gccattcttc attgactgct tcatagaaat
     5761 cttgtttcaa tcgttctttg ttcattcatc gatcgctcct tttttgtttc gtgtgcccat
     5821 tatcttacaa aatgaaggaa ttaacaaata gaacataaaa aattgaaaat tcgaacaatc
     5881 aaactgattt gtttttttca atccttcact tataatgaaa aaaaggaggc gatcccaact
     5941 gaatcaagta gtggataaac ctttatattt caaagtagat aaaaaaatta aaaaattagc
     6001 cagcactcag cagctgcagt caagaaaaag taaattgctt tttttagctc tggtttttga
     6061 agatcaaagt tatgtgatta tcgatcaatc aggacatccg gtagaatatt ctccagcaaa
     6121 atatacttat caagagggtc tttcttgtaa aaaatggaag ctgatcaatg aagagccaat
     6181 cgagttatct agatggatca acagaaaaga agatattccc gttttgatcg aagaaaaaag
     6241 gtcaggaaag gaactcgcaa attgctgggt gggattgcca gaagaacgtt ttttgaggta
     6301 taagaaatgg gctaccccct caggctatct ttgtggtact tacgcggcag cagttctcct
     6361 tgcctattat caagactatc gtaaggggtg gatgcttcca aatgagattc gcaagaaaaa
     6421 tacggctgat tcgttggttt tgacgaaggc attgcgtagc cagatccaac cactaggact
     6481 tcctacgatt ccttttcagg ttagtacagg gatttctagt tttttaaaga agaatggcaa
     6541 tcatgagcgt gcacgagcaa ctcttctagg ttcatggcag cgagctacaa aacgtatacg
     6601 tgaaggcaaa cctgtgatga ttggtatttt gaaagtactt ggcagtacat atggcaatca
     6661 ttgggtgaca gcgtacgctt attttgaaac tgaaacagga gaacgttatt acaaagtaca
     6721 cgacaactgg ggcgattatc ataaagtcat tcctgccagt tggagtaacg gaacagtttc
     6781 acttccttaa gaatttgctg aaattaacag aaaatcttgg aaaaatatgt taaactatcg
     6841 aagaatagag aagttttggg gaggactaca tgagtaataa gtatcaggat gacacgttga
     6901 agatttttag tttgaatggc aaccgtccat tagctgaaaa aatcgcgaaa gtgttcggaa
     6961 cagaattagg taaaagcgtc gtgaagcaat ttagtgatgg cgagatttcc atcaatatcg
     7021 aagaaagtat tcgaggggat catgtgtata tcgttcaatc aacgaatgca cctgtcaatg
     7081 attactatat ggaactactg atcatgatcg atgcaatgaa gcgagcaagt gcgaaaacga
     7141 tcaatgtcgt attgccttac tatgggtatg ctaggcaaga ccgcacggcg aaacctcatg
     7201 aaccgatcac tgcgaaattg atcgctaact tgatcgaaga agcaggggca actcgagtac
     7261 tgactttaga tctgcacacg gttcaagtac aaggattctt tgatattcct gtagataatt
     7321 tatttacgat gccgttgttt gctcattatt accgtcaatt aggtctgacg ggagatgaca
     7381 tcgtagttgt ttcgccaaaa aacagcggtg ttcagcgtgc acgtagtctt tcagaatatt
     7441 tgaacagcac attagccatc gtagatcatg cagatgatgc ttcaactgag ggtggttatg
     7501 tgatcggtga tgttcaagga aagacatgta tcatggtaga tgatatcttg aacacaggag
     7561 caacgcttgc acgagcagca aatgtattaa aagaaaacgg cgcaaaagaa gtatatgcct
     7621 gtgcgtcaca tggattattg tccgatcatg cgaaaagtat tttagacaat gcaccaatca
     7681 aagatatttg tatcactgat tctgttttca cagaagaaaa tcgtcatcca gaaaacttaa
     7741 caatcgttac atgttcagaa ttaatgggtg aagcggtcaa acgcatccat gaaaatacac
     7801 cgatgagtcc gttattccgt ctggaagaaa aagaattcga ataaacaaaa aaaagaggga
     7861 aagaagttgt gacagaagtg agcagcttca aggagctgcg acaagacttt tgtcacagct
     7921 ccttttttct atgcccatga aacgtcctct agacaaaaat gagctcttct ttggtttgtt
     7981 cgctatttta tgctagaatg tgcctagcaa taatttggaa gggatgaatg aagtgaaaac
     8041 tatttatctg gatcatgcgg caactacccc catgcatcca caagtaattg aagcgatgac
     8101 ggcaatcatg caagacacgt ttggaaatcc ttcaagcata catggatttg gtcgccacgc
     8161 gcatgaaaaa ttggaaacag caagacaaat cattgcagac agtctgcaag caaaacctca
     8221 cgagatcatc tttaacagtg ggggaacaga aggggataat acagctatct tagaaacagc
     8281 gttctcgcgt caaaaagaag gcagacatct aatcactaca gctatcgaac atcctgctgt
     8341 tcttaatacc atgaaatatt tagagacaaa ggggtttgac gttacatatc tgcctgtaga
     8401 tgaaaaaggg aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag atacgatctt
     8461 ggtctctgtg atgtacggga acaatgaaat cggaaatttg atgccaatca aagaaatcgg
     8521 tgaactttta gcggatcatc cagcttattt ccatacagat gcggtacaag cgtacggaaa
     8581 ccaaacgatc catccagaag aattaggaat cgatttatta agtatctctg ctcataaaat
     8641 caatgggcca aaaggcgtcg gtttcttata taaacgagac ggagtaaata tccctgcatt
     8701 gcttttaggc ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat
     8761 ctggggtatg gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa
     8821 caaatataaa ggattccagc aattgattct tgatacatta gaagcaaacg aaatccctta
     8881 tcaagtaaat ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc gcttaattgg
     8941 cgaagcaaat gatttgctac tgatgaagct tgatcttaat ggggtcgcta tttctactgg
     9001 ttcagcttgt acagcaggaa atatcgagcc ttcacacgta ttagaagcaa tgtatggaga
     9061 agatacaccg atactaaaag aatccatccg tatcagcttt ggtttgggaa ataccgaaga
     9121 cgacatccag tatttccttg atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc
     9181 attcttatat gaacagatgt ctgagagaac gccttcattt tttagggaac ttaactgaaa
     9241 aacctagtaa aaatcaggat aaacttttat aataaagaga aactaatttt atcgaggtga
     9301 aaatcatggc atttgaaaca acagcaacgg tactgggcgc accaattagc tatcgtttgc
     9361 atcctgatgc aaaaagatat acattgcgcg ataatggatt tacagaaaca aacggaggca
     9421 attatcaatt gatccgacca ttagatgcca cgccgcaaag caaagaagga ttcaaattaa
     9481 aaatcactgt atcaaaagat attcaaacat tgaaaatgtc gatcactaca gcaaatggat
     9541 tgaaagcagt caatattttc aaagatccaa aacagaaaat gagccaagat aaattttatt
     9601 ttctgatgga tgggatgatt agccgtgggt tattcgaaaa agtcgaatga tctgtggata
     9661 taaaaagaca actagttttg aaacggccta ttgttagatg actaactttg taaggacgac
     9721 gcaaattagt tgtctttttt atttttaaat ttaacacatg ttgttatttt taatattttg
     9781 ctgatttatt gtttctaagt aaagaaattt aaattgaaac gattgttatt tagggaaaaa
     9841 ggtatttttt gaaaaaagaa tcccgtataa tgattgttgt gcaaacgcta tacaaaatga
     9901 aaaagaaaag agggatgttt atggaaaatg aacagtcggt cgttttaacg aattggaaga
     9961 gaaattattt gttcttttta tcgggacagt ttttgtcagg aatcactagt atggtcgtcc
    10021 aatatgcgat catctggtat ttgacaagag aaaccggttc ggcgacaatt ttgagttttg
    10081 ctactttact aggaatgatt cccatggttt tactaagccc atttgtagga ccgctagtgg
    10141 atcgctggga taaaaaagct cttttgattg tcacggatat tattgtagcg attttcgcgc
    10201 ttattttagc agtagtcgga acgatttcag aatccttccc gatttggcta gtttttgtgt
    10261 ctttgtttat gcgttcggtc gcacaaacat tccaaatgcc aacgatccag tcgattatgc
    10321 cgacaatcgt tccatcttct cacataacaa ggacgaatgg acagttagga atggtccaat
    10381 cagcgaattt catcattgcg ccagctctag gtgcggcttt gttttcagtc gttcctgtca
    10441 attaccttat tttattagat gtattggggg cagtttttgg tgtcggctta ttgatttttg
    10501 tgaagatccc aaaagtttct cctgaaatat tggaagttcc gcttactatt tttaaagatg
    10561 caaaatttgg gctgcagcaa ttgatggata ataaagggtt atggtacata acgatcaatg
    10621 gtgcatttgt gatgctgttg ttcatgccag caatcagtct gtatccattg atgacgctag
    10681 attactttgg tggttccgtt ggacaggcgg gagcagtgga agtagtctac gcagtcggaa
    10741 tgctcttagg cggtgcactg atcagtttta taggaacatg gaaagaccga atgaaaccta
    10801 ttattatagc ctacatcatt atgggcctga cgatcggtgc aagcggattg gttccaaacg
    10861 atagtcaagg gttcttgtac tttcttatct tgaatgcagg agcaggttgt gcaacgccat
    10921 atttcaatac actgctgatg gcaatgatcc agcagagtta tgaatcgaat gttttaggtc
    10981 gtgtcttagg gaacttcaac tctttgatga acctggcagg tccaattgga ttattatttg
    11041 caggaccatt agctgatcgt ttaggggtag aaaaaatgtt cttgttttcc ggaatcggga
    11101 ttttattatg cggaatcgtt ttatttttaa cgtcagctgc ccgaaattat gataaagaat
    11161 tacagaaaaa actggtaaaa gaacaccatg agcaaaaaga cgaatagtat ggaatggagt
    11221 tgtgacagaa gtcttgttac aactccattt ctgatcctaa tgttcctgtg gcaataagcc
    11281 tactcaaatg aaaaatatga gtaattatct ttatttgttt aatacttgat atagggcagt
    11341 tttttcacga cctatcgtac ttcctctttg cttcatagat ttctttgatt tgttgctcag
    11401 ataacggagt gccaaatggt agtttttcct ttaagagttc tctttccggt gcgtctacgc
    11461 ctacattgac atcgttctcg ataggtacgc tgtaatgatg gatatgtcca tgaagattgc
    11521 ggatagtgtc gttaggcccg agcaacatcg gataatgagt caagtaatat tgttgatggt
    11581 cgaatttaac gatggcacct acatcataaa aataaaattt gggacttccg tcagtaagcc
    11641 taggatcatt tttttctaaa taacggaaaa aagcgcgact gtcatggttc cctttgatca
    11701 agtggattgt tccttgcagt tgagtgacca gttccaaaat ttctgcattt gctttttcat
    11761 attgcggatg catagcaagg tcgcctagat gataaacatg gtcggtttct tttattactg
    11821 cattccagct agtgatcaac tgttggttca tttcatccac atttgcaaag agccgcggag
    11881 caaagtcgtt ttttcctaat aagcgttcat ggaaaaagtg catatcactg ataaagtaat
    11941 tcatgtatct tttgcctccc ttttttctta gagtaaacaa aatcacttat ctgagcaact
    12001 cataacatct aaaaagaaaa ggttgtaaat gagacgtgca gctttaagcg ataagaagca
    12061 attttgaaaa atagtttttc acaatttata aaattattcc ttattgccga aaggctggcc
    12121 ctttttatga gtatgccaat tttttatatc gaatgctcaa aagatgagtg attttttggt
    12181 tgactatcat gtttgtgtac tgaataggtc cagtagaaca gtatttttac gacttgataa
    12241 ttaagggatg aaattttcag aaatatgacg tataatgtaa aatactgaaa agttgcgacc
    12301 ttcaactcgt agatgtttca gaatctaatt gaaatgatgg tgatatcatg acagacaaca
    12361 gcaaaacacg cgttgtcgta gggatgagcg gcggtgtcga ctcatctgta acagcacttt
    12421 tgttaaagga acaaggatac gacgtgatcg gaatattcat gaaaaattgg gatgacacgg
    12481 acgaaaacgg tgtatgcacg gccacggaag attataagga tgtagcgaaa gtagctgctc
    12541 aaatcgggat tccttattac tctgtgaatt ttgaaaaaga atattgggat cgtgtatttg
    12601 aatatttcct agccgaatac cgcgcaggac gtacacctaa cccagacgtg atgtgcaaca
    12661 aagaaatcaa attcaaagca ttcttggatt atgcaatgga tcttggcgcc gattatgtag
    12721 ctactggaca ttatgcgcaa gtcactcgcg atgaaaacgg cgtggtccac atgcttcgtg
    12781 gagtagacaa taataaagat caaacttact tccttagcca attatcacaa gaacagcttt
    12841 caaaaacgat gttccctcta ggcggcatgg aaaaatcaga agtacgggca attgctgaac
    12901 gcgcaggcct tgcgactgct aagaaaaaag attcaacggg aatctgcttt attggtgaaa
    12961 agaacttcaa acaatttttg agcaactatc tgccagcaaa aaaaggaaat atggtcactc
    13021 ttgatggtga agtcaaagga caacatgctg gcttgatgta ttatacgatt ggtcaacgtc
    13081 aaggactagg gatcggtggc ggaggagatt cgcaagaacc ctggtttgtt gtaggaaaag
    13141 atctagcgac aaacacatta tatgtggggc aagggttcca tcatcctgca ttgtatgcca
    13201 caagcttgga tgcaagtgaa atccacttta cgacgaatga accaatgcca aaagaattca
    13261 agtgtacagc gaaattccgt tatcgtcagc aagacgttcc tgtgacagtt cgcttattgg
    13321 atgataatcg tgcagaagtc gtattcgacg aacctgtacg tgcaatcact cctggacaag
    13381 cagttgtatt ttatgatgga atggaatgtc tgggtggtgg tttgatcgac cacgcatatc
    13441 aagaaacaaa agttttacaa tacgtttaag cgatattaaa tgaatcaaaa cagctatggc
    13501 taacaaaaga aacacctttt cttttggata gcatagctgt ttttttgcaa taaaattaat
    13561 ttttattcat tttatacttg aaatacttgt gtaaaaagtt cataatatcc aataatatga
    13621 ataaagttgc tttttgttaa gcaagagcag ttgcaaaata gaagggaatc ttttgcaagt
    13681 gatttgttca attttataag tgagggtgat ggaaatggaa aagaaatcga cgggtattag
    13741 caaatgggga ctggttgctc tggttgtcag ttcgtcaatc ggaagcggtg tgtttgggat
    13801 cacaagtgat ctggctagtt ctgcagctcc agggccagcg atcctttcat ggataatcgt
    13861 tgggattggt attttagctt tggtattatc gctgaatcat ttaggagaaa agcgtccaga
    13921 tctagatggt ggtattttcg gttacgcgga agcttcattt ggtaaattag gtggcttcat
    13981 cagtggttgg ggttactggt tgtctgcgtg gctgggaaat gttgcttttg caacgatgct
    14041 gatgagcgcc atgggagaat ttttcccgat attcaaaggt ggacaaaatg ttccttctat
    14101 tttatttgca agtattttta tttggggtct gactttatta gtaaacaatg gagttgaagg
    14161 agcgagcgtc atcaatacaa tcgtgacgat ctgtaaatta gtgccattat tcctattttt
    14221 aatatttgta tttatcgcat ttaaagtgaa tatattcact gctgattttt ggggtaacgt
    14281 atctgataat ctagtgaatg gaacagggca gcaaggaacg atctggttgc agattaaagg
    14341 ttgtttgatg gttatgatgt gggtctttgt agggatcgaa ggcgcaagtg ttttagccaa
    14401 ccgcgcgaag aaacgttcag aagcccagca agcatcgatc attggtctgc tttgtttatt
    14461 gtttatctat atccttgctt cgatcttgcc atacggcgtc ttatctcaag aagagttagc
    14521 aactatttca caacctgcta tggcaaatat tttaaaagga atcgtgggaa attggggagc
    14581 agcgctgatc aatatcggct tgattatttc gatcattggt tcttggttgt cttggacgat
    14641 gctcccagca gaaacaacga tgttgatggc tcgagacggt ctgttgccga aaggttgggg
    14701 aaaaacgaat aaaaaaaatg ccccaactta ttcgttagtt gttactgcaa tcttgactaa
    14761 tctattctta ttgactttct tagttactga ttatgcctat caatttgcct attctttatg
    14821 tacagcggca attttgattt gttatctact tgtcgggatc tatcaagtcc agttttcttt
    14881 aaaagaagga atcaaaaaac aagtagtgat cggtgggatt gctgtagttt ttgaattgat
    14941 gggcatcatt ttagcaggat tttcttatgt ccttttatgt agtattgcct atcttcctgg
    15001 tttttgcttc tactggatcg cttgtcgtga aaccaaacat caaattacgg gaaaagaaaa
    15061 agtaatgatt gggctgattt gtgtggccgc actgctttca atcgggttgc ttgctgtagg
    15121 ctggatcaag atataagggg gaaagaaaca tgaaaatcgc aaatttcggt gtagaagaat
    15181 ggctaaataa atgggaaaag aaagcggtat atgatattgc acaaagctcg atcgaagcat
    15241 tgacattaga cgaactcgtt ggcttagatg ggactagcgt aagtaaattt ttcgaaaaac
    15301 gcaaaaacga accattggac tatggctgga tcgaaggatc agatgttttc aaacaagaag
    15361 tcgcttctct ttatcaaacc gtcgatccgg aaaacatctt acaaacgaat ggagcgactg
    15421 gtgctaatct tttagctcta tacgcgttag tggaagcagg agaccatgtt gtttcgatgc
    15481 ttcctactta tcagcaatta tatgacatcc ccaaatcttt aggtgccgct gttgactttg
    15541 ttcatttgaa ggaagaagaa gattggcagt ttgatttaga acagctagaa gccttagtca
    15601 aacctgaaac aaaaatcatc tgtttgaata gtgcaaacaa tcctacaggc acattgctag
    15661 atcggaaaac tttagaaaaa atcgcagaaa ttgctcgtac agtagatgca tatgtattga
    15721 tagatgaagt atatgcgcct ttaacggata aaggtgaatt cttgtcgatc gtagatgtct
    15781 atgaaaaagg tatagcaacc aattccctat ccaaaaccta ctccattcca ggtattcgta
    15841 ttggctggac agctactgga cctgagttgg cagaagtttt tcgaaagtat cgagactaca
    15901 cgatgatttg cggcggagtc ctgtcagatg atcttgctgt acatgcattg aagaataaag
    15961 aaaaaatatt ggcgcgaaac aaaaagatca ttacagagaa ccttgtgatt ttaaaacaat
    16021 gggtagccaa tgaaccgaaa gttgaacttg tcgctcccaa ttacgtctct acttctttca
    16081 ttaaattagc catcgaagaa gatgaccaga cattttgtat caatttatta gaagaaacag
    16141 gagtactatt agtaccagga agtgcctttg atttaccgaa gcatgcccga ctaggttact
    16201 gttgtaaaaa agaaaccttg gaaaagggat tattcctctt atctgagttc ttgaaaaaac
    16261 aggcctaaac aaaaacactg caaggacagt ctgtgacaac tgttcaagca gtgtttttca
    16321 tttacgctta ttgtctatga gaaggagagt gccctgaagc tatggagact cctgaagctt
    16381 tttcttctgc agtttcttgc tgatggatct cttgtgtatt gtgtcgtaca agcaagaagt
    16441 tgaccaaaac aagaagagaa gtcgaaagaa agacgccgcg ataaccaaat gcactggaaa
    16501 cactggagcc gatcataggc cctactacgt taccagttgc ttggaaagat tggttataac
    16561 tgaagatccg tccagcagct tgatgaggcg aatattttgt gatcaatgct tggacagcag
    16621 ggagcagaca agcatcagag ataccaatca aaaaacgtaa ggcagctaat tgccaaacat
    16681 tcgtgacgaa tgccattggg atataaacca gaatcgcaaa acccaaacca atcgcaagta
    16741 ttctttcact cccaatcttg tcacctaagc gaccaaatct tggagcggct atcaaagtgg
    16801 caatcccagg tatcgaagca atgattccgc tgatcaacgt aacattccca tgtccatgca
    16861 gcaattggcg aatgtacaga ctgatgattg gactaatgga attgttggat gcttggatga
    16921 tcatcgttgt gacgaacata ccgatgacga cgtgtggata tttcaagtct ttgaaaatct
    16981 gtttggcaga aaccatgttt tcttttttca ctgggacaaa tttttcatgg acgaaaaaca
    17041 gactcaacaa gaagacgaaa aataagatga tccctgtaat gaaaaaggta gggcgatagc
    17101 caaacgctga agcagagatc ccgccaagaa gcggtccaag taatgttcct gtaacagaac
    17161 cagttgctaa ggttccaaga acttgaccac ttttttcttt tggcgtccca gttgcgacaa
    17221 gggcagtggc attactgata taaccagaaa aaatcccttg aagcagacgc aatgcgacta
    17281 attgatacac actagtcacg caacccatca aagaaatgac gattgccatt cctaatgaag
    17341 cacgcaacag catcaatttt cttccttttt ggtcggctaa ttttccccac caaggagaga
    17401 taagcgtagt caccaagaaa gtagatgaaa aagtcaatcc gctccaaaag ttcaattgtg
    17461 acgtactata attacctaac gtatctatgt aaagtgacat aaaaggcatg actaaactaa
    17521 atccgatacc agccataaat gttccaaacc ataagacaaa caagttttgt tcccaagttt
    17581 ctcttttcct aaataatttt tcctttattc ttgcagccaa ttagactcct tctttctgaa
    17641 aattttcatc tacagctctt tattataaac tacaatatgt agtttatcaa tgaaagtctc
    17701 ttttttctct ttctttttcg tggtaaactg aaagagtgaa ggatttattt tatgagagga
    17761 aacatggaaa tgaaaaaaag aaatctgtct caagagaaaa tcatcgactg ctttcgagaa
    17821 ctagcagaag agatggatgt tcagcaaata acctttcagc atttagccaa acgcttggat
    17881 atcaaggctc cctcgctgta taaccatttc aaaaatattc gtgatgtcaa aacagcgcta
    17941 acagcgaaac ttctgcagga acttaatgac cagctgcgac gggctcttgt gggaaaaagt
    18001 ggtcaggaag cactgcagat ctatgcagaa acttatcgat cctttgcttt ttcaaatcaa
    18061 gcagtgtatg agttactgat cagtgtaccg cataccaatg aagctgtttt attagatgga
    18121 atccatgaaa cgaatcagat cattttgcag atatttgagg cttttcaatt aacgaaagaa
    18181 gaaaaagttc atcgaagcag ggaacttcgt agtatgatcc acggttatct ttccttacgt
    18241 tttttaggat actttacaaa agaaccaaca atcgatccag aagacagcta tttttggatg
    18301 atcaataact ttatcgctac tttgccttaa acaatcagat tcatttgtaa caattttaag
    18361 atgttcatta aatgaccctt ttataagaga aaattcttgc aactttcttg aaaagtgact
    18421 aagatgagtc tagaaaagct gaaagaagga gcacgagtat gtatcaagta attaccatgt
    18481 tcggggacaa tgagccttgg tggttttttg aagactggga agaagatatt gaagaagaag
    18541 aaacattcga gtctttcgaa gaagcacgac aagcgtacga aaaacaatgg aacgaaatcc
    18601 atcaaaatta tgaatatatc aatgcaaaaa gtaactttct cagcgccttt tggaatgacg
    18661 gagatgagcg ctggtgtgaa gagtgtgacg atgatttaca gcaatacaag ggacttgcgt
    18721 tgttgaaaaa tcatcagccc atcacagtag aaagcagaaa ggaattttat gaaacaacta
    18781 attctagcgg aaaaaccaag cgttgcgaaa gacctaagca aggtgcttgg tgctaatcaa
    18841 aagcacaaaa attattatga aggacccaag gtgatcgtta cttgggccct tggtcattta
    18901 ttaggattga aaatgccgga agatctgaat aaggaatggc agagctggca aatggacacg
    18961 ttgccaatga tccctaaaaa actaggaatc aaaccacttc ctaaaacagg acatcaatta
    19021 aaagcaatca agcagctggc tcagcgaaaa gatgtctcag aagcagtcat tgcgacagat
    19081 gccggaagag aaggggaact tgttgctcgc tggatcttag agtatgtgca tttcaataaa
    19141 ccagtgaagc ggttatggat ctcttctcaa acagataaag caatcaaaac tggattcaag
    19201 caactaagac ctgcgaaaga atatgatgca ttgtatgaat ctgctcttgc ccgggcaaaa
    19261 gcggactggc tggtaggatt gaatgtgacc agagccttaa ctgtgaagta cgcggataat
    19321 ttatcagcag gccgtgtgca gacaccgaca ctagcattag tgagagacca agaaaagaaa
    19381 atcgagcagt tccgtccaca aacgtatttc acgatcacgt tgcaagctgg aaaagaacaa
    19441 gccaaaatgc ttcaaaaaaa tcaatttgct ttaaaaacac aagaagaagc acaaaaactt
    19501 gttcaattga tcagtacaag caaaggaact gtcacagaag tccaagaaaa attaaaaacg
    19561 gaatctgcgc cattgcctta tgatttaaca gagatccaaa gagaagccaa tcaacgctat
    19621 ggctattctg caaagaaaac attaagtctt gtacaaagtt tgtatgagac ccataagatc
    19681 gttacttatc cgcgtacaga cagcaaatat ctgacaaatg acatgaaaag tacgatgaaa
    19741 gaacgattgc aagcagtcag cgactttgct cctgaggtca agcagtattt gaaaaacgga
    19801 gccatcgtcc aacagacaaa agtattccaa gacagtaaag tgaccgatca ccatgcactt
    19861 ttgccaactg aaaacagagc acgttatgaa aaattaagta atgaagagcg aaaaatctat
    19921 caaatgattg tctctcgttt tttaggactg tttgctcaac cccataaagt cagccaaaca
    19981 aaagttacgg ttgaatttgg taaagaacga ttcatttttc ggcaaaatcg tgtcgtccaa
    20041 gcaggatgga aaggcgaaac tgaaactgag acggaaacag tcaaatggga aaaaggaatg
    20101 agaatcaatc ccgatttcgc catcaaaaaa gaactatctg caccgcctaa accattgtca
    20161 gaagcaagct tgcttggtca aatggaaaaa tacagtctgg gaaccccagc tacgcgtgca
    20221 gagattatcg agaaactaat caaatctgaa ctgatggaac gtacccctca aggactgcaa
    20281 gtatctccta aaggtaagca attattagta ttggtaaatc cctctttagt aacaccagat
    20341 ttgacagaaa gctgggaaaa agaccttgaa gctattgctg caggaaagaa acaagcagga
    20401 accttcttga atggaatcga aaaagaaaca aaacgcttgg tgaatgagat caaatcaagc
    20461 aaacaagaat atcaagattt ctcgattacc caaaagaaat gcccagaatg tggggccaat
    20521 ctgcgcgaga aaaacacacg tgacggcaaa atctatgtct gcaccaattc agattgcaac
    20581 taccgcagaa gaaaagatcc aaaagtctct aatcatcgat gcccgcaatg tcaccggaaa
    20641 atggaaatca tcgaaggtaa aaacggtgct tatttccgtt gtaaatacga tggaacgaca
    20701 gaaaaattgc ttgataaaaa agagcagaag aagaaaatga ccaaacatga agaacgacgc
    20761 ttgctgaaaa aatatgcaca agcagaagaa ccagaagaaa gcccattagc agcagcacta
    20821 aaagcagcaa tgaagaagta ataagatatg aaagaggttg tgatagaagt ggtcagcttc
    20881 aagaaataag aaggaattta cgaaaattgc ttctcaaatt tttgtgaaat ttcggcttat
    20941 ttccgaagaa gctacttctg gaacaccatt tattcggaaa aaaggactgt gacaagactt
    21001 ttgtcacagt cctttttcat ttaaaaaaat gtttacttca tttgacgtat attattggtg
    21061 aggcttcaat tcatttggct tcactttttc tgtttctctt atgatataaa caggacgctg
    21121 tttcgtttct aagaagattt tcccaatata tttcccgatg attccaagac ataacaattg
    21181 gattccacca atgaacagga agatcgagac catggaaggc catcctgatg taggatcacc
    21241 aaacaacaat gcccgcaaaa taatgaacaa aattgcaata ccagaaccga cacaagacaa
    21301 agcgccgata aatgaagcaa tcgttaacgg tgcatccgag aagttcacga taccatcaat
    21361 cgagtagttg aataatttcc agaaggacca agatgtttcg ccagccacac gttcgcggtt
    21421 ttcataagaa atatattctg ttttaaatcc gacccagcta aataatcctt ttgaaaatcg
    21481 gttgtattct gttaattcta ggacggcatc caccatttga cgagtcatca agcggaaatc
    21541 acgagcgcca tcgaccattt ctgtatcgct gattttattg atcaactggt agaatttttt
    21601 cgcaaagaaa ctgcgtatcg caggctcgcc ttcacgagtc gaacgccggg tgccgacaca
    21661 atcgatatca gaagtagtga tccggttgat catctcagga agcagttcgg gcggatcttg
    21721 cagatcaaca tccatcacgg tcactaaatc tcctgtagaa gcttgcaatc cagcgtacag
    21781 agccgcttct ttcccaaaat ttctggaaaa tgagacaaag cggacataag gataagctga
    21841 ggctagcttc ttgattgctg ctaacgtacc gtcttttgat ccgtcgtcaa taaacaaata
    21901 ttcgaactca tgggcacctt tgaatttttc aacctcatca aaaaaaagag gaacagtttt
    21961 ttcttcatta aaacatggga caacaattga tactttcatc gtttctatcc tttcaatcgt
    22021 atgtttatat atggtttaca ttcaaaattt aataaacctt aaataacacc tttaaaattt
    22081 gattttacgt aaaagaacag gtattctttt tgtaaactaa cgtatttagt ttaccatgtt
    22141 tcctcggttt atcaaacttt taagaacaat ttttattagt tggcaagtag ttttggatag
    22201 agaaaaagga ggaagttcca gtctattgac taggagcaaa caaaatgaaa atacacacac
    22261 ctatcggctt tttatggtta gatgcagatg aatccggatt gactgctgta tcttttcagc
    22321 caatttcaga aagcgaatca gcgaatcagc gaattttaac gcgcacacaa gaacagtttg
    22381 aagaatattt tgcgggaaaa agaaaagaat ttgatgttcc tttatctata aagagaggta
    22441 cgccttttca gcaaaatgtc tggaaagctt tgtcgaccat tccttatgga gaaatccgaa
    22501 cgtataaaga agtagccgtt gctgttgata gtccgaaagc tgtgcgagcg attgggcagg
    22561 caaatcgaac caatccattg ccaatcgtta ttccttgtca tcgagtgatc gggcaaaatg
    22621 gaaaactgac aggatatatg ggaaacgcaa tagatggggt tgaaatcaaa cgccaatt
//
LOCUS       DAZBSA010000041        22423 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000041 DAZBSA010000000
VERSION     DAZBSA010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.041"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACHGMT_001457"
     CDS             6..305
                     /locus_tag="ACHGMT_001457"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN6835205.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACHGMT_001458"
     CDS             378..1739
                     /locus_tag="ACHGMT_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFN6835206.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACHGMT_001459"
     CDS             1761..2021
                     /locus_tag="ACHGMT_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835207.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACHGMT_001460"
     CDS             2035..2787
                     /locus_tag="ACHGMT_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFN6835208.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGKKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACHGMT_001461"
     CDS             complement(2881..3486)
                     /locus_tag="ACHGMT_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN6835209.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACHGMT_001462"
     CDS             complement(3539..4513)
                     /locus_tag="ACHGMT_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFN6835210.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACHGMT_001463"
     CDS             complement(4891..5610)
                     /locus_tag="ACHGMT_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN6835211.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHGMT_001464"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHGMT_001464"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFN6835212.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACHGMT_001465"
     CDS             7161..8558
                     /locus_tag="ACHGMT_001465"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN6835213.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACHGMT_001466"
     CDS             8639..9079
                     /locus_tag="ACHGMT_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFN6835214.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACHGMT_001467"
     CDS             9227..9553
                     /locus_tag="ACHGMT_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFN6835215.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACHGMT_001468"
     CDS             9554..10303
                     /locus_tag="ACHGMT_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFN6835216.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACHGMT_001469"
     CDS             complement(10383..10838)
                     /locus_tag="ACHGMT_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFN6835217.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="ACHGMT_001470"
     CDS             complement(11265..11540)
                     /locus_tag="ACHGMT_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835218.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="ACHGMT_001471"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="ACHGMT_001471"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFN6835219.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ACHGMT_001472"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ACHGMT_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFN6835220.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="ACHGMT_001473"
     CDS             14770..17436
                     /locus_tag="ACHGMT_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFN6835221.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="ACHGMT_001474"
     CDS             17800..18552
                     /locus_tag="ACHGMT_001474"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN6835222.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="ACHGMT_001475"
     CDS             complement(18574..19728)
                     /locus_tag="ACHGMT_001475"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFN6835223.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            19913..22354
                     /locus_tag="ACHGMT_001476"
     CDS             19913..22354
                     /locus_tag="ACHGMT_001476"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFN6835224.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         7199 a         3933 c         4672 g         6619 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtaaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagctg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat tgttaaaatt taatcttaga aaaaaataac tggaacaaga
    19861 gctatttgtt tttacagcaa agaatggtat atttttgtag gaggtggctt acttgaaaga
    19921 agtcgttatg atagatgctg caagaacgcc gataggaaag tatcgcggaa gtttaagtcc
    19981 atttactgca gttgaattag gcacacttgt gactaaagga ttattagata aaacaaaact
    20041 gaaaaaagat aaaatcgatc aagtaatatt tggaaatgtc ctgcaagctg gtaatggtca
    20101 aaatgttgcc agacagattg ctttgaacag cggtctccct gtagatgtcc cagcaatgac
    20161 gatcaatgaa gtttgcggat ctggtatgaa agcagtgatc ttagcacgcc agctgatcca
    20221 attaggtgaa gcagaacttg tgatagccgg cggaacagag agtatgtctc aagcacctat
    20281 gctgaaacag tatcagtcag aaacaaatga atatggtgaa ccaatttcca gtatggtcaa
    20341 cgacggattg actgacgcat tttcaaatgc acatatggga ttaaccgcag agaatgttgc
    20401 aacacaattt tctgtgagca gagaagagca ggatcgctat gccttgtcgt cccagttgaa
    20461 agcagcacat gctgtcgaag ccggtgtatt ttctgaggag atcatcccag tcaagatttc
    20521 tgatgaagac gtgttatctg aggatgaagc agttcgtgga aatagtacat tggaaaaact
    20581 gggcacgtta cgtacagtat tctcagaaga aggaactgta acagcaggaa atgcttcccc
    20641 gttgaatgac ggtgcctctg tggtgatcct tgcatccaaa gaatacgcag aaaataataa
    20701 tctgccttat ttagcaacca tcaaagaagt agcggaggtc ggtattgacc caagtatcat
    20761 gggaatcgct ccaatcaaag ccattcaaaa attgacagat agatcaggaa tgaatttatc
    20821 aacaatcgat ctttttgaga tcaatgaagc atttgcagct tcttctatcg tagttagcca
    20881 agaactgcag ttagatgaag aaaaggtcaa tatatatgga ggagcaatcg cattaggcca
    20941 tccaattggt gcaagcggtg cacgtatctt gactacatta gcttacggat tgcttcgtga
    21001 acagaagaga tacggtattg cttcattatg tattggtgga ggtttaggct tggcagtctt
    21061 gttagaagcg aatatggagc aaacacataa ggatgtgcaa aaaaaaaagt tttatcagct
    21121 tacgccatct gaacggcgca gtcaattaat agaaaaaaat gtattgacac aagaaactgc
    21181 gttgattttt caggaacaaa cactttctga agagctctcc gatcacatga tcgaaaacca
    21241 agtgagtgaa gtggaaatcc caatggggat cgcgcagaat tttcaaatca atggcaagaa
    21301 aaaatggatt ccgatggcaa cagaggaacc atctgtcatt gcagctgcaa gtaatggtgc
    21361 aaaaatctgt ggaaatatct gcgcagaaac acctcagcgg ctgatgcgag ggcaaatcgt
    21421 tctatcagga aaatcggagt atcaagcagt gatcaatgca gttaatcatc gaaaagaaga
    21481 gctgattctt tgtgcaaatg aaagttatcc ttcaatcgtg aaacgcgggg gcggtgttca
    21541 agatatctct actcgggaat tcatggggag tttccatgca tacttatcta ttgatttttt
    21601 agtggatgta aaagatgcaa tgggcgctaa catgatcaac tcgattcttg agagtgtggc
    21661 aaacaagtta cgggagtggt tccctgaaga agagattcta ttcagtattt taagtaattt
    21721 tgctacagaa tcattggctt ctgcctgttg tgaaatccca ttcgaacgct tagggagaaa
    21781 caaagaaatc ggtgaacaaa ttgccaaaaa aatccaacag gcaggggaat atgcgaagct
    21841 tgacccttac cgtgcagcaa cgcacaacaa agggatcatg aatggtatag aagctgtagt
    21901 ggcagctaca ggaaatgata cacgtgcggt tagcgcttcg atccacgcct acgcggcaag
    21961 aaatggatta taccaaggat taacagactg gcagatcaaa ggggataaac tagttggaaa
    22021 actaacggtg cctttggctg ttgccacagt aggcggagct tccaatatcc tgccaaaagc
    22081 aaaagcatct ttagctatgt tagatattga ttcagccaag gaactagcac aagtaattgc
    22141 tgcagtaggg ttagctcaaa acctggctgc tttgcgtgca ttagttacag aaggaatcca
    22201 aaaaggccac atgggacttc aagctcgttc attagcgatc tcaattgggg ctattggtga
    22261 ggaaatcgag caagttgcga aaaaattacg tgaagcagaa aagatgaacc agcagacagc
    22321 tatacaaata ttggaaaaaa tccgggaaaa ataaaaaagc aatatcttta gaagaataaa
    22381 gcaagattta cagttatttt atttactgca gatcttgctt ttt
//
LOCUS       DAZBSA010000042        22326 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000042 DAZBSA010000000
VERSION     DAZBSA010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.042"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..403
                     /locus_tag="ACHGMT_001477"
     CDS             5..403
                     /locus_tag="ACHGMT_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFN6835225.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1081
                     /locus_tag="ACHGMT_001478"
                     /pseudo
     CDS             387..1081
                     /locus_tag="ACHGMT_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            complement(1675..2562)
                     /locus_tag="ACHGMT_001479"
     CDS             complement(1675..2562)
                     /locus_tag="ACHGMT_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFN6835226.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2747..3586
                     /locus_tag="ACHGMT_001480"
     CDS             2747..3586
                     /locus_tag="ACHGMT_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFN6835227.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3782..4756
                     /gene="sgrA"
                     /locus_tag="ACHGMT_001481"
     CDS             3782..4756
                     /gene="sgrA"
                     /locus_tag="ACHGMT_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFN6835228.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5054..5410
                     /locus_tag="ACHGMT_001482"
     CDS             5054..5410
                     /locus_tag="ACHGMT_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835229.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5455..6570
                     /locus_tag="ACHGMT_001483"
     CDS             5455..6570
                     /locus_tag="ACHGMT_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835230.1"
                     /translation="MKNYHLSCSLKKGNPIEGEEYRIRYFFHLMYWQSFDENHSDILS
                     SIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSITYVPEEMNKFVHPL
                     ITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYLEEDLLDTPDKVQES
                     WGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGTKKKVDAFGKSAEDR
                     DFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCMLVRDIFIKHEQPVIF
                     YIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYPIDKKAYDQTNTQIF
                     YWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6596..7036)
                     /locus_tag="ACHGMT_001484"
     CDS             complement(6596..7036)
                     /locus_tag="ACHGMT_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFN6835231.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7196..7702
                     /locus_tag="ACHGMT_001485"
     CDS             7196..7702
                     /locus_tag="ACHGMT_001485"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFN6835232.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7870..9783
                     /locus_tag="ACHGMT_001486"
     CDS             7870..9783
                     /locus_tag="ACHGMT_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFN6835233.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9788..10789
                     /locus_tag="ACHGMT_001487"
     CDS             9788..10789
                     /locus_tag="ACHGMT_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN6835234.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(10991..11197)
                     /locus_tag="ACHGMT_001488"
     CDS             complement(10991..11197)
                     /locus_tag="ACHGMT_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFN6835235.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11843..12691)
                     /locus_tag="ACHGMT_001489"
     CDS             complement(11843..12691)
                     /locus_tag="ACHGMT_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN6835236.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12855..13757
                     /gene="gnd"
                     /locus_tag="ACHGMT_001490"
     CDS             12855..13757
                     /gene="gnd"
                     /locus_tag="ACHGMT_001490"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFN6835237.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            13759..14760
                     /locus_tag="ACHGMT_001491"
     CDS             13759..14760
                     /locus_tag="ACHGMT_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFN6835238.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14757..15041
                     /locus_tag="ACHGMT_001492"
     CDS             14757..15041
                     /locus_tag="ACHGMT_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFN6835239.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15074..15871
                     /locus_tag="ACHGMT_001493"
     CDS             15074..15871
                     /locus_tag="ACHGMT_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFN6835240.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15885..16718
                     /locus_tag="ACHGMT_001494"
     CDS             15885..16718
                     /locus_tag="ACHGMT_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFN6835241.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16736..17593
                     /locus_tag="ACHGMT_001495"
     CDS             16736..17593
                     /locus_tag="ACHGMT_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN6835242.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17615..18124
                     /locus_tag="ACHGMT_001496"
     CDS             17615..18124
                     /locus_tag="ACHGMT_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFN6835243.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18161..18562
                     /locus_tag="ACHGMT_001497"
     CDS             18161..18562
                     /locus_tag="ACHGMT_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835244.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18674..19027
                     /locus_tag="ACHGMT_001498"
     CDS             18674..19027
                     /locus_tag="ACHGMT_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835245.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19061..19504
                     /locus_tag="ACHGMT_001499"
     CDS             19061..19504
                     /locus_tag="ACHGMT_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN6835246.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19521..20606
                     /gene="uxuA"
                     /locus_tag="ACHGMT_001500"
     CDS             19521..20606
                     /gene="uxuA"
                     /locus_tag="ACHGMT_001500"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFN6835247.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20599..21312
                     /locus_tag="ACHGMT_001501"
     CDS             20599..21312
                     /locus_tag="ACHGMT_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN6835248.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(21519..21668)
                     /locus_tag="ACHGMT_001502"
     CDS             complement(21519..21668)
                     /locus_tag="ACHGMT_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835249.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            22064..22264
                     /locus_tag="ACHGMT_001503"
     CDS             22064..22264
                     /locus_tag="ACHGMT_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN6835250.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         7689 a         3431 c         4623 g         6583 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcagcgata acgttaggta aagagctact
      961 agatcaagta ttagagatgc cctcgattga taaagcagtg aggggcttcc aagaagattg
     1021 ggtgcaaact ttccaagcag aaacgattgg tgaactaaaa aaggtcgctg attacttctg
     1081 aatcaatttc tccataaaat aaacgatagc cttctcgaaa actcgaggag gctatctgac
     1141 gattcaatat tcattaattt aaagtttggg tgactattac taactattac ttatttctca
     1201 tttaaacgat gtattcccta acctctaata catccttgta actctgaagt tacgccagtt
     1261 ttgaataaat ttcataaaaa caaatgaaaa gtgtcgcata aaaatatctt tcgtgtttct
     1321 ctagtataac agaataagaa gaacaagtcc agaagggata tttttagtct tttttaaaat
     1381 aacttttcag tcgagtttga gtttatgata gttgtttttg aagcgatacg gcaagtgaaa
     1441 aaatagggaa gcatgaaaga atctacttaa tggcgcattg attcatgtga tagaaataaa
     1501 aaactttttt aaattatatc ctcatgattg taacaaatcc agtgatttgt tttggtacaa
     1561 ataaaaaaag gccccgatga agttgtggca aaagtggttt gcctcaaaaa taagaaggaa
     1621 tttccaaaaa tcacttctca aattttgcca catccccaat tgggtcaaat ttgtttattt
     1681 gaatatattt tttacataag gatctttaat agttacattg tcttttttca tcactttacg
     1741 gattgtttta tccatgaatt ccgtatcaag agtttttgct tcggtagcaa ttttttctag
     1801 ctcagatttg tatttgtctt tattagaacc tttatcctgt ttttttacca tttttactag
     1861 ataatagctt tgctgataag tcgttggatc tgttacagga atgatatcag aaacttcccc
     1921 gttttttagt ttaaatgctg ctttttttac ttcagtagga atctctggat tggttgaatc
     1981 aaaattcatt tttccatcag tttcctttaa tgttttattt tttccataag cttgaacaag
     2041 cttggagaaa ttttctcctt catttgcttc tttttttgct ttatctgctt cttctttagt
     2101 cgaaaataga atgatctgtg tactaacttc tggatagaat tctttccaag cagtgtctag
     2161 atctttatct gttaatttta cattggcttt caaaccgtat tggaaagcca gcatctgttt
     2221 aaagagtttc ttatatgtct cttctgtttg gccaactgct tttaattgtt ccttgtagtt
     2281 tggaccgagt tttttgattt gttcattata ttcttttgtt acttcctttg aagaaatctc
     2341 ttttccgtat acatcatcaa atgctttgaa gacaatcatt ttttttaata gtgtttgatt
     2401 atttgattta acttttgggt tatcataaaa atcctgaact gtaagtgttc cacctttaaa
     2461 cgttgcaatc gtttcattgg acttgctaca acctgagaat agtaaaattg cacatgctga
     2521 aaggcacata attagtagtt gttttttcaa aaaatttccc atcctttcct tattaaaaac
     2581 agtctaacag aagaattagg gagacagtct ttcattttcg taaggttgct gtagatattc
     2641 ttagttcgta aaaatctatt ctctctatga tggagaacat ttacatagtt ttcacgagac
     2701 aagaaagaaa agggttgtta aacttaatat aaaggaggga tgaaagatga tcaggattat
     2761 tcggttgatt ggatctgttt taatagtctt tgttggtttt gttttaggaa ttcttgtcaa
     2821 taatgcactt gcggatatgt ttattggaaa tagtgaatgg attggagcaa ttgccggtac
     2881 ggtcggacat ttagctgttt ttttactagc tttatttctt gctagtaagg tcgaaggaaa
     2941 aagaatagaa gattatggtg tttctgttcg aggaaaagac tgggggtatt tagcgggagg
     3001 tctcgttgtt ggaattggag tgtttctgct tattactagt ccattatatt tgacgggggc
     3061 gtatcggtta gattcaggaa acgcaaacat tgtgccgctg ataacgagtt ttattttatt
     3121 tattgcagtg ggggcatcag aagagctttt attccgtggc ttttttcagc atcagatgct
     3181 tagatttggt ccgttaatag caatgattgg atctgctgca ttatttgctc ttttgcacgg
     3241 attgaatcct aacatgacat ttctggcagt tttcaatatc tttttagcag gttgcttttt
     3301 cagcgcactt atctacagta caggaagttt atttacggct attggtgctc atattacttg
     3361 gaattggaca caaggagcta ttttgggtat accagtcagc ggaacgcaag aacaaggttt
     3421 tttctccact ctgatccaat cgcaaaatac aataattact gggggaaact ttggggctga
     3481 agcatctata tcaactacat tattcttgtt agtcttgatt gttggtttgt tactctatgc
     3541 atataaaaca aagcggatga agaaatacac tgctgtttat caataaaaga tgaagaaagc
     3601 aaggctgata tcggtcttgc ttttttaagt ctgtcattga ttaaacgaaa aagtaaacaa
     3661 attcaagcga atacacgaca tcttttgtta gattttcaca actgactgtc gtgttctcat
     3721 cattttcatt ttatataatt cctttatcaa atagaaggta attatatgga gagtgataga
     3781 aatgaagaaa acagcgaccg tattatttgt tattcttggg ctgagtataa ttgcagcaat
     3841 gattggaatc agtacttctg caaatgaacg ggcaaatgag aatctttcat ttactgtgaa
     3901 aactgatcgg attgtttatg acatgacaca acaagtcatt actattccag tcaaaccaaa
     3961 caaaagcgtg aatgctagtg atgtacatgc tgttctaaca tatggttggg atggtaatgg
     4021 aagttcagaa aaagttattg gggaagttta tttaaaagac gtacaatgga ccgctggtat
     4081 tgagtataca ataatgattt ctgcagaact atcgattgat gagatcaaga gtaaagataa
     4141 agttgatttg attgtttttt atgatggaca gatgacgatc acggagaatt tgaaaccaag
     4201 cagttggaca gttgttggac cgccaagcag tacggatagc acaagtagtg aaagcagtac
     4261 agaaaacacg agcggtgaaa gcagtacaga gagtacgagc agtgaaatta gcacagaaaa
     4321 cacgagcagc gaaagcagta cggagagtac gagtagtgaa agtagcacag gaagcacgag
     4381 cagcgaaagc agtacggaga gtacgagtag tgaaagtagc acaggaagca tgagtagtga
     4441 gagtagcaca ggaagcacga gcagcgaaag cagcacagga aaagtaaata atgaacttga
     4501 tttggtacct atcataccaa ctaaggaaca aaagagaaat gcagggagca acctaaaaag
     4561 tgtgctaaaa cagcctgatt ttgtttctcg tggaattgat catcaagaaa aagcgaaaga
     4621 aacagataat agaaaaagcg atttaccaga aactggtagt agaaccttga acaagctaat
     4681 tacatggatg ggtgtgttac tcattttgat tgtaggagca agctattttc gttctctctt
     4741 ccatcgagtg aaataaagaa taaaagcaac agatctaaaa ttgaataaaa cgatagattt
     4801 ttgagtgaat atttcattcg agagtgctgt attgcctggc gaagttgtag ctttgccaga
     4861 tctatttttg tttactaaca tatttgagaa acaggaaata acaggaacac aaatggaaaa
     4921 taagagcaag aataattgac aatgcacact gcgcatggta gtatctctga aaaatcagta
     4981 acgaatctat ttgtacgtta ttcatgtagc tgtcggaata agtctaaata atagataaaa
     5041 aggagtatgt actttgtttg aagaatttat tgatatcaat gaaaggcaag tatatcaatt
     5101 tttgaactat tgttatgaaa gagacgagaa attatatgtg gtgaaggata tcgctttaga
     5161 tctaaactac accctggcta agatgaattc ggtgattcaa caagcagaat cgttttgcga
     5221 aaggtatcca gaatataaat tatcttttct ttcagaaaac aagatgataa aagtcgagtt
     5281 ttcttctcag tttttactga gtaaagttta ttctatactt ttagagggaa cgattggtta
     5341 tattttactg gattcacttt acaaaggtac ttatcaatcc ttagaaaatc ttagtcaaaa
     5401 aattatataa gtttgcgtac gattcaaaga aaattaagag aacttaatac tattttgaaa
     5461 aactatcatt tatcttgcag cttgaaaaaa ggtaatccga tagaaggaga agaataccgt
     5521 atccgatact tttttcactt gatgtattgg cagtcattcg acgaaaatca ttctgatata
     5581 ttatcaagta taaaaaataa acgtcaacta ttagaaaaat ttgcgaaaca tgcgccatat
     5641 acgcgtagta tagatagaaa gaaatggatc agcctactaa gaatttcgtt gcaacgaatg
     5701 aaacaaggcc actcgattac gtatgtccca gaagagatga acaaatttgt gcatccattg
     5761 ataacaaaag aagagtttac gcattacata ctagagcctt tttttgaagc aaacttctgg
     5821 tcgatgaatc acatcactcc aacagagcaa gcttaccttt attttatgtt tggtgtaatg
     5881 aacacctatt tggaagaaga tttgttggat actccagata aagtacaaga aagttggggc
     5941 tggattgaat cgtctattgt agaaaatttt gaaaaaaact tcaagatttg ttttacaact
     6001 tcagaaaaaa attatctata tgtcaatttg gcaatgatcc atttgtattc aagggttttt
     6061 gggacaaaaa agaaggtgga tgcttttgga aaatcagcag aagacagaga ttttcagcat
     6121 atatttccag tgatgtatcc aatcatgcaa cagtttttta gtggggtagc tcgcagaatt
     6181 ccagagctta atacttatta tgaaaagaac agacggttga tttttcaata ctgtatgtta
     6241 gtcagggata tttttataaa acacgaacaa cctgtcatat tctatattca atcaaagtat
     6301 ggtaaaacac aggaattatg ggcaaagaaa aggatattat ctatgactga acgcccaata
     6361 aaatatagtg catccgctga tcagttacct gatattgtca ttagcgatta tcctatcgac
     6421 aaaaaggcat atgatcaaac gaatacacag atattttact ggaatggaca acctacaaaa
     6481 aatgattggg cgcgtctgaa agctgaaatc ataaaaatca ggaatgagaa aaatagatcc
     6541 atattggaaa aattggattt cgttaattaa gaaaagcaga ttgtttatgc caaaattaag
     6601 acattgcaaa aagattattt tctttaaaat cgacaagatg accagaaagc aagagttcgt
     6661 tgagctcttg ctttctttta gttttagaaa ggaacatcct gattttatct tcgctaaatc
     6721 cagtcaaaag ttttgcagat tggtcgtaga taatcggttt tttcaataaa gaaggggatg
     6781 tacgaatctc aatatatagt tcactcaaac tgagttcatc tattctgtga acaagatttt
     6841 tataactaat agaatgagta gagagcattt cggtcacgcc gttgtttgtc aattttagta
     6901 agtggataaa ttcatttttg gttagtggtt gacgaattaa gttctgttcg ataaacggta
     6961 tatcatggac gataagccaa cgttttgcgc gtcttgaaga cgagcagcca gaacctgtat
     7021 acaatttaat catcatacat tcctccaata gctaagtatt tatgaaataa taacatgaag
     7081 aaatagtcga aaagtgccat ttagaggtgt aaaataagaa aatgttgtca cgtataatgg
     7141 aactagctag ataagaaata ctattaaatc aagaaaaaag aaagaaggca caagtatgtc
     7201 aaacacagcc gacgcaatag tagtgatcga tttacaaaaa ggggtatgtt ttgattcttc
     7261 gccgatacac gaattagaaa cagtgacagc attagtgaat aaaagaatca aaacatacga
     7321 aacgattggg aaaccagtta ttttcgttca acacgaagat gctcaattag tgccacaaac
     7381 aaaaccatgg gagctattgt cggaattatc tattcctgag cacagttatt ttgtgagaaa
     7441 aacccatgcg aattcatttt ataagactaa tttgagtgtt ttattaactg aattggactg
     7501 tcattcgatc gaattatgtg gggcacaagc agagtattgt atagatacga ctgtgaagtt
     7561 tgctcatgga ttaggatacc agcttatgat gtgtagagga gcaacgacaa cttatgataa
     7621 tccttttatg aaagcaaagg aaacaatcgc tttttacgag aaaatttggg ataaacgatt
     7681 tctgacattt ttggagcaat aagttgtttc ttcgtttaaa aaaagcagaa tggatactgg
     7741 tttatccatt ctgctttatt acgtaaaatg agaaaataat cctgttctat ttttatggga
     7801 taaaccattt attttttgta taaaaaatgt ttgcgtttac atataattct cctagaaaca
     7861 taaaaaggag tgaatagctt gagaacaaaa caagtattaa agggagcgtt ggtcatctta
     7921 tttgctgtaa gtacaagcgt cccgcaatgg acagtatatg gagagagcat ttcttccgaa
     7981 tcggaatctc gggaggtaga aaccacagaa actactgatg ctggaaatga aacgaaatcg
     8041 agtacttcac aaacagaaac aaatactgaa acaacggttg agacatctgt tactagcaca
     8101 gatcaagaaa cgaccgctac cacagataac caacaaacag aagaagcaac agctacaaaa
     8161 atgtcaaacg acgaatcctc ttctgctaaa gtaagtgaca aagagaatgg gacagaaaat
     8221 gatgatatcg gtaaaggtga aaatttcaat ggtaaagaat atttgaatta cttcggtaaa
     8281 cagatgacag aaatacttaa tggaccaaca gaagacatca agtcaaaatt aatagagtta
     8341 ttagtcactg agcttgctgt taatgattat ggtgtagata aagagattat caaacatctg
     8401 attgctactt atctattgta cgatatcgct tttgcaaaaa acagtgtgtt gatgtcgatg
     8461 cttggaggta cagatgatgg gacaacgcct acagaaaaca caggcttcta tccaaataca
     8521 aaacaacttc atcaaactgt actaaacaaa caagatggca aaatgatgga tgtatatgca
     8581 tattatgtcg atcaaggttc agataaaaca gtcatgatcc atggcggatt tcgagggaat
     8641 tggaataacg ggatcgttac agaagaatac aatgattttt ataaggccgg gtataattta
     8701 ttatttgttg attccagagc gactggtaat agcggaggcg attatgtgac ctatggacaa
     8761 tatgagtcag atgacgtcct ttactggata aatcaagaag ttcgagagcg gccatctcaa
     8821 aagattttac tttatggtgg ttcgatggga gcagctacga tgatgtctgt attagcgaaa
     8881 gatattcctg tcaatgtcaa aggaattatt gaaaattgcg gatttgcatc aattgatgaa
     8941 caactacgtt ttacgtacag ccagacagta gcccccgcac taccggatgc cataaaaaat
     9001 cagttagaca tcattgggga tcaagagcat gaagatttat ttatgggcct tttgaaacaa
     9061 tactattttg atcaggagat gcatttggat acaaaagctg ctctacctac gattggaatg
     9121 tcagattcat tgcctaaatt gattatccat ggaacagcag atgatgtagt acctgtgtct
     9181 aacgcgcaaa aattatatga gttatcagga gggtataaag atcttttatt agtagaagga
     9241 gcagggcatg gaaaggcaca ggaagttgac catgctgcct acacgaaaca tgtaacggac
     9301 tttttgaaag tagtgttcca tgatcagata aacgtaaagt atgtggatga aaacaataaa
     9361 agtctactca aagaccagga cgaaatccag ctttacggag cttatggaga aaattatatg
     9421 acagagcaaa agacattcga aggatacgaa ttagcaaatg ttgaaggacc gactgaaggg
     9481 atttttaatg agacgatacc aactattatt ttctattaca aaaaaatccc agtagctcca
     9541 ccaaaaaagc aagatccgac agagaatgca gacaataagg gaaagaatga acctcgagag
     9601 gatcaatcaa gtgatgatac gcaaccgatt cagatgacag tgaaagtaca aaaaaagaca
     9661 gatgcaaaaa aaagaatgtt gccgaaaact ggagaaaaga aacattcgct tctcatttat
     9721 tcaggtggat ttttatctgt tagcacgatt atcacatgga tttggcgaag aaagagaggg
     9781 taaatgaatg aaatggttca agcgaatagt gattgcagta ccagttatca tcgtagttat
     9841 tttagtcact gtatttttga ttaatcaaat cacaccaaag ccgattagct tgcttgttag
     9901 aaaacaattt gacacaagtg aaaaagaaca agtatatgcc cgaccagatg atttccagca
     9961 aaaaatcgaa caaatcgata taaaaaaaga aatatcctat ccttcaaagt acaaaaataa
    10021 taatatggat atctacacac cgaaagtgga aaagaaaaaa ttaccgattc ttttctggat
    10081 gcatggaggc gcatatgtag gaggggataa aaatgattgt cgtgattatt tggaatatct
    10141 atgttcagac acgcagcaaa tcatcgtaaa tatagattat gaacgatcac cggaagcaaa
    10201 acatcctaca ccagttatcc aattgaacga agccattcaa gcagctaaaa aagagataaa
    10261 agatcaagca gattggtcga atatttcgat tggtggaaat tcggcaggtg cacaaattag
    10321 tggagaatat ttgttagcac ttcaaaatga agaaattaaa aaagctgatc atcttgatcc
    10381 gactttggag aacaagcaga ttacgaagtt tatttctttg tctggtctgt tggatccttc
    10441 tgcacttact caagtaagtg ataaaatctc tagctttctt tatgaaaaat gtggatgggc
    10501 ttactttgat aataaggact ttgaacaatc agagaaggtc aaaagtttag ccttggtcag
    10561 acatgcggat cgttggacgc aaaacacatt cttaacggat ggaaatacaa atacgtttac
    10621 gaaacaaatg gaagaagtaa cttctgtatt tgagaaagcg ggggtgaaag tcacaaaagt
    10681 agattatgcg gagaaaaatg ctgttttgaa ccatgaatat cagtttgatt tcagcaataa
    10741 acaagcgcaa gaaacatatc aaaagttagt aatgtttttc aaggagtaaa gtcttatgat
    10801 gcaaaagagg ttgtaatctg tcacttagct ccaagtcaca aggagcaatt tcaataaaca
    10861 agcttttcat ggtttatgaa tattgtaact tgtgactgag gagttagaga tggcaagggg
    10921 caccttttat tcgaaatttg ggaatatcac acgattattg tcacgatctc ttttacgtaa
    10981 attttataag tcagatttgt tgttttgatc gtttaaaaaa agcaacaaat ccgaagcctg
    11041 cggaggcaag aagggacaaa aagccgagtg gtaagcaaaa gaatgctggt tccactaata
    11101 ttccgttgtt attgatataa ggctgggaac aaagatgaat agcaaataaa atcaatgcac
    11161 tagcgaaaaa aagcaaagaa atatggatag ctttcataag cagcacctcc ttttttataa
    11221 ttgtatgata aatagattaa gaatctataa atgtgacaag tttgttagga tgtgaataaa
    11281 atgatcatct agcgaacttg aaacaaagat ggctaatgat aagcaattaa tgattcagca
    11341 atcagatagg tacaaagttt accatcatac gtctagacca ataatagaaa atatgggaat
    11401 ataatagaaa agatttagga tacccccaaa agaatttccc caaaaattcg atgtatccta
    11461 aactttttta ttttacataa caatcatatc aactgtgaaa taaaattaca aatgataacc
    11521 taaaatgagg ccaaaagcca gtgttatcaa ggaaatactt aattcaccaa aatttttttt
    11581 aattttttag aaaataagaa acaaaaataa taaaaaaaag aaaagataaa ttttttacta
    11641 ctgtatacta actttgttat cgcatctaac aagtaactcg aataatacca atacaacctt
    11701 ctccaaagat gcgatgacat acgttctaac ccaccgacta ttgatgttct gtaccaccct
    11761 tgcagaacca aaaaagccac taccaaatat ttggtagtgg ctttttttgt tttatggatt
    11821 tttcgctttt tctgttatgg tgctattctt tgatttcaga aatagtatgt cgaactttag
    11881 cgatagaacg acgtgattct ttttcgttgt aaaacatcaa aatcacatat agagagtcca
    11941 aaatgcttag ttgagaaata cgggaagcta aagcttcaga tcggaattct gtttcttcag
    12001 aaatagaaat aaaaacgaca tcgccaagtt tagcaaggga agaattagct tggctagtaa
    12061 taacgattgt ttttgctcca gtttttctta cggtttcagc aatatggatt gtttcttttg
    12121 attgccctgt gtgagaaatc aaaacagcac agtctttttc cgtcaataaa gaagcttcca
    12181 tcaattgaat atggtaatcc gtgctgtgag aagttgcgat aggggaacgc aaaaatttat
    12241 gataagcgtc agttgctaca atctctgaac cgccaacacc gaaaaaatag acacgtttag
    12301 attcattgat aatagtagcc gcttttttta gatccttttg ttgtaacaac ttttttgtgt
    12361 catttaaagt tgaaatacta gactcgaaca ccttttgcgc catagtgagc tcagtatctg
    12421 ttttctgaat attttcgtgg atagaaatag cagatagatc attttcttgg atcaacatgg
    12481 ccattttaaa atctttaaat ccattataac caagtttttt tgtaaattga aaaaaagtag
    12541 agtcagcaat gcccagttca cttgctaaat tgctaatagg actatgactt actttgcttg
    12601 gattttctaa aatataatcg gcgatagcct gttcttttgg gctgaagtcg ttgtacattg
    12661 ttttgatttg tagtcttatg gagacgctca tgagaattcc tcattttcat aataagatta
    12721 tcattttttc cgttctcatt aatgttaaag atacaaggag agtaggaaaa aatcaaacaa
    12781 aaaatatttt tgtaaaatct atttacaaaa aaattttttt gtataatgag ggtataagaa
    12841 atggaggcgc tatcatggaa attggtgtaa ttggtttggg aaaaatggga ttgaatttag
    12901 ctttaaactt aaaagagcac catcacaaag tacaaggact cgacatttca agtacagctg
    12961 ttagtcaggc aagagaaaat gacattcctg cttatcaaga gatctctgaa tttctagcta
    13021 cttttgagca gaaaaaaatc atctggttaa tgcttcctgc tggagagata accgaaaaca
    13081 tgttaagaaa actttttcca aaactcggac caggagacat tattattgaa ggcggaaact
    13141 cttattataa agattcaatc cgtcgagcag aagagtttca gaaaaatgaa atcggttact
    13201 tggattgtgg gacttctgga ggaatcgaag gagcacgaca tcaagcctgc ttaatgattg
    13261 gaggagataa agaaactttt ctatccgtgg aacaattatt caaagatgta gcaattgaaa
    13321 gaggatatct gtatgctggt gcatccggaa gcggacactt cttgaaaatg atccataatg
    13381 gaatcgaata tggaatgatg caagcaatcg gtgaaggatt ccaacttgta caaaaaagtg
    13441 agtatgcttt tgacttggaa caagtcgcac gtgtttggaa tcatggttcg gtgattcgcg
    13501 gctggctgat ggaaatcatg gaagatcagt taaacgaaca tcccaattta agtaattatc
    13561 ggggaatcgt ttctgcatca ggtgaagcaa aatggacggt tgaaacagcg ctagaaatgg
    13621 ctgttccagt tccagtaatt gcaactagtt tgttcatgag gaatctttca caggaagaag
    13681 atagtttttc agcaaaagta gtttcagcac taagaaatgg gtttggcgga catgaaatag
    13741 ttgaaaaggg tgagtaaaat gccagattat catatagcaa tcgttaattc cagcagtttc
    13801 ggaagagtct ttccacacca cttggaaaga ttaaagtcag ttggagacgt tcatttcttt
    13861 actgttgatc aagagattcc aggaaaagaa ctagcggagt tacttaaagg attcaatatc
    13921 attattgcaa gtgttacgcc attttttcga cgagattttt ttgaaaacaa agatgaactg
    13981 ctattgattt cccgacatgg tattggttac aacaatattg atttaaaagc agcaaaagaa
    14041 caccagacaa tcgtatctat catacctgct ttaattgaac gaaatgctgt agcagaaaat
    14101 aatataacta atttgttgtc catgcttcga cgcacgacac aagcacaagc aagagtcaag
    14161 gacgatcgat gggaagatcg ggcggagttc gtcggtagga cgctgtttaa caaaacagtt
    14221 ggtgtgattg gtgcaggaaa taccggaagc ggagtaatcg aaattttgcg taacggtttt
    14281 cgctgtgatg ttctagctta tgatccttac aaatcagatt tatacatcga aagctttggt
    14341 gcaaaaaaag tagaacttca agaattactg gaacaatcag atatcatttg tttatgtgcg
    14401 aatttgacag aagagaacta tcacatgatt tctgataaag aagtcgatca gatgaaagaa
    14461 aatgtttatc tatctaatag tgcaagaggc gctctagttg atgaagaagc gatgattaat
    14521 ggtctgaaaa gcggaaagat cgcaggatat gccacagatg ttttagaaga agaaccggga
    14581 cgtcaagatc atccgtattt agcgtttgaa caagtagtga ttacgccaca tatatcggcc
    14641 tacacaaggg aatgtttaga agcaatgggt gaaaaatgtg tcagagatgt cgaacagatc
    14701 agtcagcacc ttcttcctga acgttctgtc cagacaaaga gcaactatat aaaaggatga
    14761 tgaagaaaat ggtgaacggt ttaaaagtga aaacaggccc tcaattttat ttatatgaag
    14821 aaggtggtat ttcaaaagtt tctgatctct tgaaatcata cggcgcgaaa agagtactgg
    14881 tcacccacgg aacggtatca tgggaaaagg cgctacctaa attagtattt ttgaatgacg
    14941 aaacgattca atttttttat catcgttatt ctggagaatg tagttatgct gaagccagac
    15001 gaatagctac aatcatcaaa aaaatgaaat cgatttcgta gttggcgttg gcggaggaaa
    15061 actgaccgat ctagtgggtt atagtgccaa tcttgcaaga atcgatttcg gagtcatccc
    15121 tactttagct agtaattgtg ctccatggac acctcttgct gtcatgtata aggagaatgg
    15181 acaagcagaa gggaaaacag aacactttta cagacaagct gcttttttta taacagatcc
    15241 ccaattagtg attgattccc ctgttccata tttcattgcc gggttagcag atacactagc
    15301 aaaatggtat gaatctgagc tcatcttaga acaagatttt ttgcaagatg aatcattttt
    15361 gaagctagca caagatactg cgaagatctg caaagaagag attttgaatc attctgctaa
    15421 agcaatccaa gatatggcac aaagaaaact gacatcggag ttcaaacagc tttcagagat
    15481 tatctttgct gttgctggtt tagttggagg attaggagat aaatacgcaa gaaatgcggc
    15541 agcacatgct atgcatgacg ctatcagtaa atatattccg gaaagccatc gttttctaca
    15601 tggagaaaaa gtagcgtatg ggattttcta tcaactggca ctagaaggtc gctgggaaaa
    15661 aatagacgag ttatatcctt tgtatcggga attagaactg ccggtttctc tagaacaaat
    15721 gggattattt ccgaaagata aacagataat agataaaatg gctgaattta ttgattcgaa
    15781 agagaaagtg catttgattc ctatagaggt aacacagaaa tcactgatac agtcaatgat
    15841 ggaactggaa ggttatatca gaaagatctg aaggaggagg acacatggaa acattaagtg
    15901 tagtacaaag tttgttgatt gctttatggg tagccgcggt tatgtcccgc tggttaggtg
    15961 gaggagccac gttgacctta cgtttttctc ctttgatgac tggattagtc gtcgggttga
    16021 tcatggggga agttgcacaa gcgatgattg ttacagccgc tttgcaaatg atttatatgg
    16081 gggttttttc tccaggagga tcaatgccag ctgaaccatc tattgcagca gctatcgcag
    16141 taccagtcgc attattaggg gatctgtcgc cagaagcagc gattgccgtt gcggtgccag
    16201 tcggattgct cggaagttac ttgtatcagt ttcgtttttt catcaatact tttttaggta
    16261 aatatacaga caaagctgta gaagatttga atacaaaagc tatggcccga tcaattattc
    16321 tttatccaac ggttgcctca tttttattat tcgttccgct tgttttcatt gctttatatt
    16381 taggggcgcc attgatcgct gatgtaatca aagctttaga gggaacagtc gttatccaca
    16441 ttcttgaagt agtaggtgga gggttagcgg caatcggtat cgcgacaact gtatacgtta
    16501 tcggtcgaaa agattatctg gtctttttct tccttgctta ttttatgagt gttgttttga
    16561 aatctttaga tattacgatg gttacttatg ccatttttgg gattattatc gctttgatct
    16621 ttgtccagtc gcaaaaaggg aaacaagtac agcctgccac aagcaataac aattcagcat
    16681 cttttgatga cgaagatgac tatgatgacg gattttaatt taggaggaat gggaaatggc
    16741 tgaaataatg gataatcaaa caggtgctcc tgaagaaatc acgaaaaaag atgtaacaaa
    16801 agcctatctg agatggcatt ttgcaaacga aattcctcac tcttttgaac gttacttggc
    16861 gccatcacta ctatacggaa tgatgccaat cttgaaaaaa ctttataaag atgaagactc
    16921 tttaaaagca gcatataaac ggcagttact gttcttcaac actcaattaa gctggggtgg
    16981 cggtgtgatt actggcctaa tgtcttctat ggaacagcaa agagcagaag aagagtatga
    17041 agaaaaagag atcttgatgc aggatgactt gatgtataac acgaaagcgg gattgatggg
    17101 ggcactagca ggaatcggag atgcaatcga ttcaggaacc gtccaatata tttttatcgc
    17161 aatcgcggta ccttgggcac aacagggaag tcctttaggc gcactttttc cttttatctg
    17221 ctttgggttg tatcaggttt tactaggggt atttttcgca agacaagctt tcactatggg
    17281 ccgtaatgct acaggcctaa tgcaaaacac tggtgttcag acagcgattg aaatgctatc
    17341 agttttaggg ttgtttatga tgggggtgct tgcgggaaac tatgtaaaag tagaatcttc
    17401 attgaaattt gcgatatccg gacgtgagtt tgttatccag gaaattcttg atcaaatcgt
    17461 gccagggata ttacctttag ctgtcgtaat gggtgtgtac ttgttctata cgaaaaaagg
    17521 attgaaagtc acacaagcat tattatggct gactggaata ttaattgtgc tagcaggaat
    17581 cggtatttta taaaaagaag ggaacgtgaa taagatgggt gtagtcaatt tagcgcgtgt
    17641 ggatgagcgt ttgatccatg ggcaagtaat ggtaacgtta tctcaaaaaa gtggagtgaa
    17701 ttccattttt gttgtagacg aagttgtagc aaaagataaa tttatgagag atctttacaa
    17761 aaatgcagga agccgtacag gacaaaaaac aattgtcatt acaccagaga aagctaaatt
    17821 ctattgggat gaatatcaat ttaaagaata caattgtatt ttgatcgcaa aaacagtcca
    17881 aacgatttac gacttagtaa aacacggaat ccccatggaa gaattgaata ttggagggat
    17941 tgctcaaaaa aatcctgata cagatctttt agtgacgaaa tctgtttatt taaataaatc
    18001 agatgctgaa aaattgaaag aaatgaatga acaatatggc gtaaagaata tttattttca
    18061 agccactcca tctgcaccta aaacaggatt aaaagatgta ttgaacaagt ttgatttgtc
    18121 ataaacaaat gcttagaaaa aagataaaga gggcgaaaaa atgaagaaga acgagctgtt
    18181 tcgtgattgg gaatttcgtt atcgctatat ttaccgtaag cgccgtacaa aaaagagtaa
    18241 acagcgtttt ctttcagcat tagtatcaga tatttacagt atgagaacag atgtgacagt
    18301 cattgcttac gatacgctag cctaccgatc aaaaaatatt tatgttggag atatcgaaaa
    18361 agcagagaaa gttatctgta cttattacga tacacccgtt catgcattag gaagttattt
    18421 catgtttgat tggaaagacc aaaggaaaaa aacaatctac tctatattat tgtccttcat
    18481 tttgttgttc agtttaggtt ggtggggaat gatgatatat aacaaaaatc ctcatcacgt
    18541 attcgatctg ctatccgtat aaacaggtat cactgtacta gcatttggga gctacttttt
    18601 cttattggga aaagcagctc gcggatggcc tagcagacaa acttttattc gaaatacttc
    18661 atccatatta acgatgctag aaatgatccg tacgattgat gatccgagtg tagcgtatgc
    18721 gtttgttgat gaaggctgtt atggagaaaa gggcttagac agcgtgagat cgggtatgaa
    18781 aaaagaggct attctttttt atctagactc tgttggggca gatacaccgc tacagttttc
    18841 gggaaattat ttttcaaata aagaacagtg gctgaaacaa gttgataagt tgaaagaaaa
    18901 aaacgtcaat tatattttta gtgcaagaaa aaagcaagcg cagttcttct atttgaccaa
    18961 aacagattta cgagggaaaa catttaattg gcaaaatgcc aatcagatta ttgctctttt
    19021 tcgataagta aaaagtgcaa aacaaggagt gagaagacta atgttggaaa tcatgatcgt
    19081 tacacatggt aagttgagcg aaggactgaa agatgcggcg gaagtaattg tcgggacatc
    19141 gagccatatt gaaacaatgt gcttagagtc tggggaggac atccaaacat tcggagagaa
    19201 ggtcaaagaa aaattaacta tgatcaacac agcagaaccc atgctagtat ttgttgatct
    19261 aattagcgca agtccgtaca atcaagcgct cttggcagtc aatgaactga ctgaagaaaa
    19321 gaaagcagcc acttatgtca tagggggcgt gaatctgcca atgttgttag aagcaatcaa
    19381 tcatcagatg cttcacacac cgatagaaga agcggtggaa caaattgtca aacaaggaaa
    19441 agataattta actgtttggc atgcttcagc ggaagaaaaa aaattagatg aggacgattt
    19501 ttagaaggaa ggctgaaaag atgaagtggg gatttagatg gtatggtgaa aaaggggatt
    19561 cgatcccatt aaaatatgtt cgccaaatac cgggaataaa tgggataatt ggaactttgc
    19621 tgactaaaat gcctggagat gtttgggaga tttcagaaat tgaggagttg aaagcatctg
    19681 ttgagagcca aggactggaa ctgcttggaa ttgaaagtgt agcgatccac gatgcaatca
    19741 aggcagggac agatgaacga gattactata ttgataacta tatacgaaca atcaaaaact
    19801 tgtctcgatg tggggttcga ttgatttgtt atagcttcaa accaattttt ggatgggcga
    19861 aaactgattt gtttaatgaa aacaacgatg gaagctattc tcttttatac gatcagaaaa
    19921 tcgtcgatca gatggagcca ggagaaatgt atcaattgat acacagccag tcaaagggat
    19981 tcaaattgcc gggttgggaa gaagaacggt tgaaaaaatt cgctcagctg gaaaaactgt
    20041 atgaaggaat gaccgaagaa gaattatttg aaaatctgac ttatttttta gaaaaagtga
    20101 ttccagtgtg cgaaaaagca gatgtaaaaa tggctatcca tccagatgat ccgccatggg
    20161 aactttttgg ttttcctaga gtgacgaaaa atcttggcga cttgaagaaa attctaaatg
    20221 cagtagattc tccatataat ggactaacgc tgtgcacagg atctttaggg gcaaatccgg
    20281 aaaacgattt agttgaaatc atccatgagg taggttcgcg catccatttt gtccacttca
    20341 gaaacgtgga gtttttagga gaaagaaagt tcaaagaaac ggcacatccc tctacagaag
    20401 gctcccttga tatgtatcat attatgaaag cgttggtgca agaagggttc gacggtccta
    20461 tacgccctga tcatggtaga accgtgtggg gtgaagtggc tatgcctgga tatggcctgt
    20521 atgatcgtgc aatgggactc acttacatgc aaggtctata tgaggcgata agtaaggaaa
    20581 aaccactggg agagcattat gaataaaatc aagctcttca tctttgatat ggatggcctt
    20641 ttattagaaa ctggacgttt agcttatcgt gcttatgtaa aatcagcaca aaagtatgat
    20701 tatgaaatgc gtaaggaagt ctattattta ctaactggcc aaacagaaat ggcgataaga
    20761 gaacaaatgg gcattttgta cggagaagat gttccatata tcaaatggcg agaagcaatc
    20821 aatcaatata aagaaaaaat cgtgaaagaa gataagagag tatatacgaa aaaaggagca
    20881 gaagaaattt tatcttttgc aaaagaaaga ggaatccata caatcgtggc ttcgtctaat
    20941 acacgggtaa aagtagaaat gtatttaaaa atggaaaatc tatatgcctt gtttgatcaa
    21001 atcatttcag gggatgatgt aaaaaaagga aagccggaac cagagatctt tttgaaagct
    21061 tgttcaaaaa tgaatattcc tccaagtgaa gcacttgtat ttgaagactc gattgcaggg
    21121 atcgaagcag caaggcgagc gggcattttg tcttttctca tcaaagatca tttgataggg
    21181 ttgcccgatc ataatgggaa gcataagttg aaagtagatg taagtttata tgaatccaat
    21241 aaagcagact atgaatttta tgacctgcat caagcaaaac gatttttaga aagtaaaggg
    21301 ttgtatctct aataaagagg actattttct agtcctcttt atttattgtc ctgcacgact
    21361 gagggaacag atgaaaaaga agtaattttt tattttgaag tatctacctt atttgtagtg
    21421 aacaatttag aacaaacaca aaacgcatct actgttatct accagtagat gcgtttaaag
    21481 agaagaacga gaatcgtcca tatgagggtt tccactcctt atgcagattt ttttaattct
    21541 tgcactgctt gttcgatcgt aacacctgtc gctacatgat tggtattttc tttgtcaaaa
    21601 acgacaaata gattcaaatc tgtatccaag gttactcgat attgtatatc tttgttatag
    21661 aatgtcatag ccattctcct ttctttaaat acttgctgaa aggcaacgaa cgcgttgata
    21721 acgaaattcc atttataagt ataccaccaa ttgaaaggtt ttcatagtta agaaatttta
    21781 aagtcaaaag ttgcagataa aagcaaataa acaagaaaac aaaaagtgaa tcaatcgcgg
    21841 ttattcaact ttttcttcga ttcgaagaca atagaagaca atgaatttta tatttcattg
    21901 cattatcctt gtgttattag aaaaagcatg atatattaaa tgagtaatct attttgaaac
    21961 gtaatttgag cgatatatct acatagataa aactcctttt gcctatcaaa tatttattgc
    22021 aacgaaaaaa cgtacattga tacgtatcag gaggaattta tttatgaata acggtacagt
    22081 aaaatggttc aacgcagaca aaggttttgg ctttatcact ggtgaagatg gaaatgatgt
    22141 atttgcacat ttttcagcta tccaaggtga cggcttcaaa actttagaag aaggccaagc
    22201 agtgtcttac gatgttgaag atggtcaacg tggccctcaa gcaacaaaca ttgttaaagc
    22261 ttaataaaaa ttgactttta accgtctttt cgaaagaaaa gacggttttt ttgtacgaaa
    22321 aaattc
//
LOCUS       DAZBSA010000043        22191 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000043 DAZBSA010000000
VERSION     DAZBSA010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.043"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            256..2091
                     /gene="tdc"
                     /locus_tag="ACHGMT_001504"
     CDS             256..2091
                     /gene="tdc"
                     /locus_tag="ACHGMT_001504"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFN6835251.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2396..3874
                     /locus_tag="ACHGMT_001505"
     CDS             2396..3874
                     /locus_tag="ACHGMT_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN6835252.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3840..5171
                     /locus_tag="ACHGMT_001506"
     CDS             3840..5171
                     /locus_tag="ACHGMT_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN6835253.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5285..7837
                     /locus_tag="ACHGMT_001507"
     CDS             5285..7837
                     /locus_tag="ACHGMT_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFN6835254.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7844..9283)
                     /locus_tag="ACHGMT_001508"
     CDS             complement(7844..9283)
                     /locus_tag="ACHGMT_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6835255.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9258..9440
                     /locus_tag="ACHGMT_001509"
     CDS             9258..9440
                     /locus_tag="ACHGMT_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835256.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9629..9991
                     /locus_tag="ACHGMT_001510"
     CDS             9629..9991
                     /locus_tag="ACHGMT_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFN6835257.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10178..11035)
                     /locus_tag="ACHGMT_001511"
     CDS             complement(10178..11035)
                     /locus_tag="ACHGMT_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN6835258.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11155..12066
                     /locus_tag="ACHGMT_001512"
     CDS             11155..12066
                     /locus_tag="ACHGMT_001512"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFN6835259.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12073..13724
                     /gene="mdcA"
                     /locus_tag="ACHGMT_001513"
                     /pseudo
     CDS             12073..13724
                     /gene="mdcA"
                     /locus_tag="ACHGMT_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
     gene            13826..14125
                     /locus_tag="ACHGMT_001514"
     CDS             13826..14125
                     /locus_tag="ACHGMT_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFN6835260.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14115..15791
                     /gene="mdcD"
                     /locus_tag="ACHGMT_001515"
     CDS             14115..15791
                     /gene="mdcD"
                     /locus_tag="ACHGMT_001515"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFN6835261.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15795..16388
                     /locus_tag="ACHGMT_001516"
     CDS             15795..16388
                     /locus_tag="ACHGMT_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFN6835262.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16412..17488
                     /locus_tag="ACHGMT_001517"
     CDS             16412..17488
                     /locus_tag="ACHGMT_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFN6835263.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17513..18382
                     /gene="mdcB"
                     /locus_tag="ACHGMT_001518"
     CDS             17513..18382
                     /gene="mdcB"
                     /locus_tag="ACHGMT_001518"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFN6835264.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18493..19257
                     /locus_tag="ACHGMT_001519"
     CDS             18493..19257
                     /locus_tag="ACHGMT_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFN6835265.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19746..21839
                     /locus_tag="ACHGMT_001520"
     CDS             19746..21839
                     /locus_tag="ACHGMT_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN6835266.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21864..22166
                     /locus_tag="ACHGMT_001521"
     CDS             21864..22166
                     /locus_tag="ACHGMT_001521"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN6835267.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7183 a         3790 c         4728 g         6490 t
ORIGIN      
        1 ggacagtcat tttatttgtt tgttcgcttt ttttaggtta tctattttag aagactgatt
       61 tattgaaaaa ttgttcgctt ttttgcgttg aattatcaga aaaatccgac tttatttatt
      121 tttgtttgta aaaatctacg tggatttgat gttataaaaa aaaaattctc atttttggcg
      181 ctatagtgaa gaggtgttac agataaacac atgtatctgc aagacattga ccaacaaaat
      241 atggagggaa gaaaaatgaa agatatggat atcaaggccg tctttatagg cgacaaagct
      301 gaaaatggac cagtttataa aatgttgttg aacaagatgg tagatgaaca tcttggatgg
      361 agagaaaatt atattccttc agatatgcct gcaattagcg aaggagataa gctcactcca
      421 gattatctag ccacccgcga ccatatgata gaggtcttgg atgaagtcag tcagcgatta
      481 cgcgcaggat cgatcccatg gcattcagca ggccgttact ggggacaaat gaatgctgaa
      541 acattgatgc ctgctttatt agcttacaac tatgcgatgc tgtggaaccc aaataatgtt
      601 gcattggaat cttctatggc aacttcgcaa atggaagcag aagtaggaca ggattttgct
      661 tcattattca atatgacaga cggatgggga cacattgcag cagatggttc tattgctaat
      721 cttgaaggat tatggtacgc ccgttgcatc aaatcgattc ctttagctgt caaggaagtg
      781 ctgcctgaaa aagtgaaaaa aatgtctgag tgggaacttt tgaatttatc tgttgaagaa
      841 attttggaaa tgaccgaaag ctttacggac gaagaaatgg atgaggtcaa agcagcttct
      901 tcccgtagcg gaaaaaatat ccaaagatta gggaaatggc tggtccctca aacaaaacac
      961 tattcttgga tgaaggcgtt agatatttgc ggagtcggct tagatcagat ggttgctatc
     1021 ccagttcaag aggattatcg aatggatatt aatgctttgg aaaaaacaat tcgtgaatta
     1081 gctggacaaa aaatcccaat tctaggtgtg gttgctgtcg taggaacgac agaagaaggt
     1141 caagtagaca gtgtagataa aatcgtccaa ttaagagaaa ggttaaaaga tgaagggatc
     1201 tatttctatt tacatgttga tgccgcatat ggcggctatg cgcgttcact gtttctaaac
     1261 gaagcaggag aatttgtgcc gtatgcttcc ttagctgaat tctttgaaga acatcatgtt
     1321 ttccaccact gcgtaacgat tgacaaagag gtatatgaag ggttcagagc tatttcagaa
     1381 gcggattctg tcacgataga tcctcataag atgggctatg tcccttatgc tgctggagga
     1441 atcgtaatca aacataaaaa tatgagaaat atcatttctt atttcgcgcc atatgtgttt
     1501 gaaaaatcag taaaggctcc agatatgtta ggagcttata ttttggaagg atcaaaagct
     1561 ggagcaaccg ccgccgcagt ctggacagca catcgtgtat tgccattgaa tgtgaccgga
     1621 tatggtcaat tgattggggc ttcaatcgag gcagctcaaa gattcagaga atttctcgat
     1681 catctgactt ttactgttaa aggaaaaaca atcgaagtct atccattgaa tcatcctgat
     1741 tttaatatgg tcaattgggt attcaaagaa caaggctgta cagatttgaa cgctatcaat
     1801 gaattaaatg aaaaaatgtt cgatcggtct tcttatatgg atggcgatgt ttatggtgaa
     1861 cggtttatta cttctcatac tacatttaca caagaagatt acggtgattc tccgattcgt
     1921 tttgttgaaa gaatgggtct aacgaaagaa gaatggaaaa aagaacagaa aatcactctg
     1981 ctacgtgcgg ctatcatgac tccttatttg aatgatgatc gaattttcaa tttttataca
     2041 aaaaaaatcg ccaaagcaat ggaaaagaaa ctaaacgaaa tcatccaata gaactgattt
     2101 ttgtgtgaaa gaataacaag agacatatgg agaacagaca attatttgct tcatcaaaac
     2161 aattgtattt ccccctgttt ttcatatatt cctcgccaag atattctttt ttataaaacg
     2221 ttctataagt tggatcgttc gtttcatgta tcaaacccag ttcactaaga tcgttcaatg
     2281 gatgtttcgg tgatactggg ttttgtactg aatcgatgaa accttgtcat ttctgttctt
     2341 gaacaaaaat aaaagaaaac aaaaacatat gtcgctattg aaagaaggaa taaagatgaa
     2401 agatactggt tcatcaaagc aaatcacgac catgaccttt atcggaatga cctgcgccct
     2461 tgtagcaagt gtacgaaata ttccagatgt agcagcaacg agctggacga tgatttttta
     2521 catgctggtt gcagtattat tgtacgcttt tccaatctca ttgatctctg gtgaatttgc
     2581 tggaatgttt ccccaaaaag gtggtccaga actttgggta tcgaatgctc taggaaaaaa
     2641 atgggggttc gttgtctcat ggctgttatg ggtccaaatg tttcctggga tggtgatggt
     2701 cgcttcagca ctcgctccgt tgtttggtaa tattatcgat aatgtgccgc ttggtttgaa
     2761 cagcaaattt acattagttg tcattttagt cgtttattgg atcatcactt ttttgaattt
     2821 gaaatttgat atggcaaaaa tcggcggaaa agtcggtgtt tggttaggat tgtacgtgcc
     2881 tttgacgatg atgctcttat tagggtttgc tgcgtggatc aaagttggga tcgtaccaac
     2941 cggaacatta ggcagttttt catgggacaa attaatacca gatactacga ctgcatcctc
     3001 atttgtttat tttgcgccga tcatgtttat ttttactggt atcgaaatgt cctctgtgta
     3061 tatcactcga ttggaaaatc cagttaaaac gtatattcgc ggagtatttg ccgcgttaat
     3121 ttttatattt ttcgtcaata ttttgaatgc tttagttgta gcaaatgtcg taccaaaagg
     3181 acaaatggaa ttgaataata tcgcgcaatc tatttcgatt tattgccaaa ttcttggttt
     3241 gccacatctt attgtgaatc tgtttagttt acttgtcttc attggtgtag cagtccaatt
     3301 atctgcttgg gcctcaggac cagcaaagac agtgacagaa agtgctagaa aaggtgcgta
     3361 tccaccaaaa ttcaattttt ggaagacgaa tcaatttgac gtatccaaat ccgttatttt
     3421 gactcagtct gtcattatct caatctttgc acttttttat ttgctgattc ctggcgtcaa
     3481 tcaagcattt ttgatgctcg taaattcaac tactgttatc tactgtatcg tctatgtcat
     3541 catggggatc gcagttttga gattgcgtta tacacatgca aagttgaatc gaccatttag
     3601 aattggaaaa aaagagaaaa agagcaatct tggagtttgg actgtcgtta ttgttttatt
     3661 tgcggcaatc ggattttctg tcggactgac gatgaaagct ggaacatgga tcaatctaat
     3721 tgcagtcaca gccatcagtg tcattttgtt tgtagtacca ctctgtattg aaaaaataaa
     3781 gaaaccttct tgggaacagg aagtcgccat gctgcttgaa aaagaaaaag gtgaacttga
     3841 tggaaaaacc ggcaatgaaa cggactctaa ctaatcgaaa tatccagtta atcgcattag
     3901 gtggcgcaat tggaacagga ttatttttag gttcagcaaa ggcaattgca tcagcagggc
     3961 cagctgttat tctcgtttat ttgatatgcg gtttggctat ttatggtttg atgcgggcca
     4021 tgggtgattt atttttagca aatagtacat ttaactctat cagtgatttc gttgactatt
     4081 atttaggcaa aaagtgggca ttttttgtca attggactta ttggctttgc tggcttggag
     4141 ctggacttgc ggaattaaca gcaataggat tgtatatcca tttctggttt gtacatgtac
     4201 cagctttgat aagcggaggt agcgcactaa tgatcttaat ggcagtcaac cttgttgcag
     4261 tcaagtggtt tggtgaattg gaatcttttt tctcaatcat caagatcttg gggattttcc
     4321 tttttatctt attgggtata ggattgagtg tctcagattt tcatacccag tctatcggaa
     4381 tcaaagaaaa ttttggacag atcgatgaat tactaccgtt tggctttgct ggaatggtag
     4441 tctcatttcc tatggtttta ttcagttttg ctggaattga aatgatcggc ttaaccgttg
     4501 gcgagacaag tgatccgcaa aagaatttga aaaaagcaat caactgtctg ccttggcgta
     4561 ttctcttttt ctatatagga acgattttgt cgattttatt tgttgttcca tggcattgtt
     4621 tagatttaaa agaaagtcct ttagtgacta tgctgcaagt gatccattgc caaggcggag
     4681 cagcaattgt caatctaatg attttggttg cttctttgtc ctcagcaaat agtgcagtct
     4741 acagtactag caggatttta tatcaaacag caaaagaaaa aagtttgccc cattggtttt
     4801 gtgcgctttc taagaaaggc gtacctttac acggtatttt aattacagga atgttatttt
     4861 ttgccggtct ttgtctacat ttttttgtag ctgatagtcg attgtttttt cagcttttgg
     4921 caggcatgac aacgagttgt tttctatttg tgtggtcttc gatcttgtgt gcacatgttc
     4981 aatttattaa agaaaagaaa cggaacacta cacaaagata taaagatctt gctttgttgg
     5041 tgttttttac ttcaattgct ttcagcttgt tattcgaaag cacgacacgc tttattcctt
     5101 ttttattggt tatctggttt gtattcttaa attttgtata taaaagaata agcaatagaa
     5161 atatcagtta ggcccagata cattttgcaa aacgaattat ttcagacccc cctgaaataa
     5221 tcgatacgcc tgaaaaaact tccctgcgta taataacagt atcaattaaa caggagggga
     5281 taggatgaac tggtacagat tgacggtaga agatgtattg aaaaactatc aaacatcaac
     5341 agaaggcttg tcgcaagaag aacgacagaa acgcctgact gatcaaggat ataatgaaat
     5401 aaaagcaagg cagcagacaa aacgatggaa aaaaatagca aagcatttta ctgatctgct
     5461 gatgatcgtg ttgatcattg cctctttttt gaaatttgct tctagtgaat atattgaagg
     5521 aagtattatt ttattcgtag tgatcgtcaa tggactcgtt agttactggc aagaaagaaa
     5581 agcggaagaa tcattgaatg gtttgaagca gctgatggga caagaagcga tagtactatc
     5641 aaacgggatg caagaaaaaa tccctgcgaa agaactggta aaaggagatg tcattgtctt
     5701 acgtgccgga gatgtcgttc cagcagatat ccgattaatc gaagcacatg atttattagt
     5761 ggaagaatcg attttgacgg gagaatcaga agcatctgaa aaaagtcatg ccgttttgac
     5821 agaagaagaa tcgatcggcg accagaaaaa tatgggtttt tcaggaacgc ttgtacaatc
     5881 tggttctgca gtcggtgtcg ttgtagaaac aggaaacacg acagaaatcg gtaaaatcaa
     5941 tcaagcattg caatcggtcg aacaacaaac gacgcctctg attaaaaaaa tcaaccgatt
     6001 aaacaaacaa atttttcaag gcattctttg tttgagccta ttcttagtta tttttacgac
     6061 atttcgatat ggtatggatt ggcatatcct tctttcggct acgattgctt tggtggtgtc
     6121 aatggtaccc gaaggattgc cagctgtttt gaccatgatt ctatccgttg gagtacatga
     6181 aatggcaaaa gaaaaggcga tcatcaaagg acttccttcg gtagaaacat taggttctat
     6241 gacagtgatc tgttcagata agacaggaac attgacaaaa aatgaaatga cagttgtgga
     6301 tgtagctgca aaagaagaag cctgcgtgtt gtccattatg aaaaactgtc aggaattaaa
     6361 aacaaaagaa gaacagaaaa cagaaaattt aagtggcaat ccgacagaag ttgcgctgat
     6421 tcgacacaca gaaacagcca atctaccttt gaaaaaaata gaagcaaaga ttccgttcag
     6481 ttctgaatac aaatatatgg caacgatgca tgcggaggaa aaaggagcgg tgatttacgt
     6541 caaaggtgca ccagaagtat tatttgctaa atcaactctt tcttctgcag aacaagaagc
     6601 atggagtcag acagctgcag aatttgcgag aaaaggtcag cgtgttttag gatttgctta
     6661 taaaaaagta gattcgaaac aggaactcac gcatgagact ttaacccaat taacttttgc
     6721 agggatcgcc ggattgatcg atccgccaaa agaaagtgca gtcaaagctg taaaggaatg
     6781 ccaacaagca gggatctcag tgaaaatgat tacaggggat cacaaagaca cagcaaaagc
     6841 gattgcggaa caaattggct taaaacatac tgccaaagtt ttagaaggga tcgatttgga
     6901 tcttatgtct gatgaagagt tgattcagca agtgccaata gtcgatgtct ttgctcgaac
     6961 gacaccagaa cataaactgc gaattgttaa agctttgcag aaaaatggag aaatcgtcgg
     7021 aatgaccgga gacggcgtaa atgatgcacc tgctttgaaa agatccgatg taggaattgc
     7081 tatgggaatc aaaggtagtg aagtaagcaa acaggcagct gatatggttt taggagatga
     7141 taatttccat acgattgcaa aagccgtaaa agagggccgg cggattttgg ataatttgca
     7201 aaaaaccatc aatttcttct tgccaactgc attagctcaa ggattgatct tgatttgggc
     7261 acttatgctg aatcgcccgc tacctttgtc cccagtacag attttatggg tcaatatggt
     7321 aacgacaatc actttatctt atgcactagg gttcgagtcg ccagatccag aaatcatgaa
     7381 gcgcccacca agagatccaa agcaaggaat cctatctggt tatcatttgt ttcggatcat
     7441 ctatgtctcc ctactgatca tggttcctgc ctatttattg gctatgcagt ttgaagggca
     7501 aagcttacag caaaccattc ttctgcaaaa tatcgtctta gctcaagctg tttacatgat
     7561 caattgcaga gagttgtcca agccgtcatt gaaccaaggt cttttgcaaa acaaagcgct
     7621 ttttctttca ttaggtatcc ttgctttatt gcaagggatg gttatttttc taccagtcgg
     7681 ccagcagctg atcggtacag taagtttaac gatgacacaa cagttattga ttggagcaaa
     7741 tgtgattctt ctattcttag ttgtagaaat agaaaaaggg atcatgaaca aactgtttcg
     7801 caaaaaagaa aaaacagcta gagaatacag tcgctagccg ttactataaa aaagttttga
     7861 aagtgagttc agtcaaacga ttgagctcat ttttcgttgg aatagaagaa atgtatacaa
     7921 tagggatatc tagagaatct agagggaaat tggaaatcaa gatatctgtt ttttcaaaag
     7981 aaatagtgga aagatcggtt agttcgatag gtaagaagca gacacgtttt cctaagtaat
     8041 cttttaattc ctgctgcaag aacgccttcc atgaaggttc tccttggaaa ataaaataga
     8101 cattgaacaa attcggtttg atagaaaaaa tcgtttgttg gatcagtaga gacaagagtt
     8161 ggagaaagta atttggttca ttgatttcta tttctttttt tagatcgttc agtaaattct
     8221 ctttctgttg atacaagtgt gcatattgct gatcatatgt ttctaaaaaa cgatggtatt
     8281 gacggtccaa tgcttcaatc aatatcggcg attcatgata ttttaatatg aggagagcca
     8341 agttatcagt cagagatgtt ttttttagtt gatcaagaga cagctctgaa gctagttcct
     8401 ctacaaacgt ttcgaggacc tctaaaaatg gagaatgatg caggcagcga gcggtttctt
     8461 gtccatagtt attgttgtaa ttccaacttt ccagaaagca gaaagaagca aaagaaagct
     8521 cagtgtcttt aagataaata ccttccgtta aatacaattt tttcaatgca gagtcataca
     8581 gggaggagag tgtgctgttg gtatggatag aagaatctaa tcggcaattc gcttttatac
     8641 ggatcgtatt gataaagaac caacatgttc cgaaaatctc ttctgtttca accgctagtt
     8701 cagtttgact gaggtttctt aaaaattgaa aataatggga ttcatggatc gaataatcct
     8761 cgaaaaaata gttgttatga gtgaaaggaa tcagcaatcg atgataaaaa gtacgaatcg
     8821 cacgctcttt tccaagaata caagctgtat atttcgtcaa atcgatctgc aagtcgtaac
     8881 ttgctaaacg ctgattgatt ttagtcacat gtcttttcaa tgtcccataa gaaagcccat
     8941 ggtcggctag aaaatcaggg acagcaactt tttttgtaaa aagcagattt ttcagcagtt
     9001 cgacactgac tgattgtttg aaaaaaatct cccaaaggtc atttgttaat aaattttgct
     9061 ggttttgcaa actgattcct gcttgatcgc tgatgatgtc ccagccttcg ggcagctgag
     9121 agcgaataga ttgaaggtca gaaaaaatcg ttcgttcggt tgtccctatg atttcagcta
     9181 attcatttac ggttatcttt gtatgattca gcaattgttc tactaaagaa acatgacgtc
     9241 gaatatcttt ttcggtaatg atttttttca ttagtgaata catcaatcag actcccttct
     9301 attctaagaa tacggagaaa ataaacggtg gtcaagtttc aactgatttt tcaatcattg
     9361 tttatttttc tggagcattt actgatgata agaatttcat catgaacttg aaaaatgata
     9421 ccactaaaat caaaaaatag gtgggcaaac aataataaac attttaatag tagatggtag
     9481 caagaccaac acaggcggat tgaacattta agagttgata aaaaagaaaa atatcgaagt
     9541 atttgattag caactacttg tattcatttt tctattctta cgtggtatct ttgaataaag
     9601 cgaatcaata agaaagagat ggaagcagat gctaacgaaa aaagaactaa tcgatcacat
     9661 caatgaaaca tacaatttgt ctccagaata tatttttaaa aaatttcctg attattgcgt
     9721 attcagacat caaaaaagca aaaaatggtt tggggtcctg atgtctgttc cagaagaaaa
     9781 aatctatgat gatgcaagtg accataggat catcaatatt cttgatttga aggtcgatcc
     9841 tgaacttggc ggaattttgc gaggaaaaga ggggtatttt ccagcctatc acatgaataa
     9901 agaacattgg atctctattg atctacgtaa aatagaaaag ctagatgaac tagcagattt
     9961 gattgatgga agttttaaat caacgcaatg aatgtctagg acaatagaga agaggttgtg
    10021 acagaaatgt ttagcttcaa gaaataagaa ggaatttacg aaaattgctt ctcaaatttt
    10081 tgggaaattt cggcttattt tctaagaaat tacttctgga acaccgtttg ttcgaaaaaa
    10141 gagctgcgac aagaattttg tcccagctct ttttttttta ttttcctaaa taaaattccc
    10201 gcatcgtttt tgcaatcatt ttgatcaaag gatgatcttc tgtgaagcta gaagataaat
    10261 aaattgttcg tgtcaagggc aaatctttga tttcccgcaa gacgaaagga tcatttttat
    10321 cgtaagacca agaaactttt gggaaaaagc taacagctaa tccttgcttg acaatagaac
    10381 gtaatgtcgc aggatcatct gtcgtcgaac tgatcgtcaa ttcgatactt ttcgcttcaa
    10441 gtgctgcatc cagtgtatga cggaaggcgt tgctctttga cagcatcaag atgggggtgc
    10501 gttggatatc ttctaattgc agatgttctt ttttagcaag aggatgtttc ttaggaatgg
    10561 cgacataaac aggttctttc atgataggaa tattggtcat gccaggcttc atctccgaag
    10621 tgatgtacag tacaggatcg gtaatatcat ctggtaaatg atgttgcatg atcgttaatt
    10681 ggatatcagg atgcttttcc ataaaaattc ggaccaagtt cggaatagaa acagaggcta
    10741 cctctaaata caatatgacg gaagtaacga tcttctcatt gaccgaagac aataatttga
    10801 ttccttcttc taatgtgtct aatgtcttga ctgcatatgg ataaaattct tctgcataaa
    10861 cggtcgtctc aatgtttctg ccgctatggt aaaacaaagg gacacctaat tcgttttcca
    10921 aatgtttgat cgcacggctc aaggcaggtt gagaaataaa taattctttg gctgcttggg
    10981 taacattttt tgtttcgaat acttttttaa aatactgcag atgaagcaat ttcataatta
    11041 tccatcccat ccataccttt ttgttatggt tttttctttt tttggcattt tttattatag
    11101 cagaaattga ctaagatagc agtggaaaaa gtaagtgctg aggaggcgag gctcatgagt
    11161 acaggtttct tatttcctgg acaaggcagt cagaagttag ggatgttagc ggatatccct
    11221 gaaatgtaca tggaacgttt agaaaatgtg acggggtatc ggttagttga taaagaagaa
    11281 aactatcaag atacggtttt tatccagcta gcattactga caaaagcagt attttattta
    11341 gatgaaatgg aaaaaaacgg tattcagcct gatattgcag caggacattc gattggtgct
    11401 ttttgtgcag ctgttgcttg tcgatctttg agatttgaga atgcagcgga actagtctat
    11461 caccgcgcag tcttaatgaa agaagcttat ccaagcggtt atgcaatggg cgtagtagta
    11521 ggtgtgacga gaagcgaagc tgaggaaatt gtagcaaaaa catttgatcc tgaccatcca
    11581 gtttatctgt caaatgaaaa ttgtccgatg caacatacgt tatctgggag tatcgaaggt
    11641 ttatccgtta cgttggcaga agccaaacgg caccaagcgc aaacagcgaa gctgctgaaa
    11701 gtccctgtgc catctcactg tctcttgatg aatgatacgg tcaagaaatt caagccgtat
    11761 atgcagtctg tccatctatc tgatgctaga tgtttgtata taaaaaatac ggatggaaga
    11821 gccactcagg acgcagaaga gattcgtaaa gatttgttgg aaaacctagc tttccctgtg
    11881 cagtggaacc aaatgatgga cttggcaaaa gaattaggaa tggatctgac gattgaattt
    11941 ccccccggca atactttgac aaagctgatt catacaaaat ttggtgtaga taaagggatc
    12001 cgtacgatca atctagacca acatggtatt gatgacgcca cgtttctata tcaaaaatgg
    12061 agatgactaa ttatggaaaa accaaaccgc aactgggctt ctaaacgcga aaaaaaagcg
    12121 cagcgattgg aaaaaatcag caacttgctc aatggcaagt atgtagaaac tgaaaaaatc
    12181 gtagaagtaa tggaaaaact gattgctccc ggcgacaaag ttgtattaga aggcgacaat
    12241 caaaaacaag caagttttct ttcagaaagt cttgaaaaag tagatgcaga aaaagtgaat
    12301 gatctgcatt tgatcatgtc tagtatctca cgaccagaac atttgaatat ttttgaaaaa
    12361 ggaatcgcaa gcaagatcga tttctcttat gctggtgcac aaagcttgcg tgtcgcccaa
    12421 atgattgaag acggcacaat gaaattaggt gatatccata cttatttgga actttatgga
    12481 cgcttattca tcgatttgat tccaaatgtt gttttagtag ctgcagataa agcagataaa
    12541 gacggcaact tgtacacagg tttcaatacg gaagaaacac caacaatcat cgaagctgca
    12601 gctttcaaag atggtatcgt gatcgttcaa gttaacgaag ttgtcgattc tttaccacgt
    12661 gtagatatcc ctggtgactg ggtagacgtt gtcgtgaaag cagataaacc ttatcaatta
    12721 gaagctttat tcacacgaga tcctcaaaac atcacagaat tgcaaattct aatggcaatg
    12781 atggcaatca aaggaatcta cgcagaacac ggcgtacaat ctttgaatca cggtattgga
    12841 ttcaatacag cagctatcga attgttactg ccaacttatg gtgaatcact agggttaaaa
    12901 gggaaaatcg cgaagaactg ggtattgaac ccacatccaa cgatgattcc agccatcgaa
    12961 tcaggttggg ttgaaagcat ccatagtttt ggtggcgaag ttggtatgga aaaatatatt
    13021 gctgctcgcc cagatgtctt ttttgtcggt aaagacggca cgatgcgcag taaccgaaca
    13081 ttatcacaag ctgcaggaca atatgccatc gatatgttta tcggttcaac cttgcaatta
    13141 gattacgaag gaaactcttc taccgtaaca aaaggacgtt tatctggttt tggcggtgcg
    13201 cctaacatgg gacataatcc tggcggtcgt cgtcattcta caccagcatg gcaatcactt
    13261 cgcgacaatg atgatccatt agggaaaggc caaaaattag ttgttcaaat ggttgaaaca
    13321 tatggttcaa ataaaaaacc agtctttgtc gaagaattag atgcactagc tgttcaaaaa
    13381 caagcaggct tagccaatgc aatgcaccag tgatgattta tagcgaagat actacacata
    13441 tcgtgacaga agaaggaatt gcttatttgt acaaagcaaa aacaaaagaa gaacgacaag
    13501 cagcaattgc agcaattggc ggtgttacac ctcttggaat gactagcacg aaagaatcac
    13561 tagatagctt gcgtgcagcc ggcatcgtga aattaccaga agatctaggc atcaaacgta
    13621 gtgaagcgaa acgttcatta ttagctgcac aaaccatcga tgatttgatc gaatggtcag
    13681 atggcctgta tgaaccacca atgaaattca gaacttggtc ataaaattgg cgagaaaaac
    13741 cttttgtcgc catttaccca gatggaagaa gaaagacgaa taagcagaac gtgtatgctc
    13801 tgttttacaa aaaggggaga gatccatgga acaattagct tataaatatc caacgaaaca
    13861 gccgatcaat aagaaaatcc atgtaggagt ggtcggttca ggagatttgg aaatcttgat
    13921 gtttccaaca gaaaaaacag aatctactgt aaaaatttgt acaggaagcg atggcttcgg
    13981 cgaagtatgg aacaatgtat taacgagatt ttttgatcgt tatccaattt cagcagatat
    14041 cgtgatcaat gacttcggtg ctacaccagg agttgtgtac ctacgattga cgcaagcaat
    14101 ggaggaactt tcagatgaaa aatagttttg tagaattaaa cgccagagaa cgagtgaacg
    14161 ctctacttga caatggacgt gaattgattg atccattcga tcaaatcatc gcgccaagtt
    14221 taccagcaca aggaatcgtc cctgaaagtg atgacgggat cgttgtcagc cgtggcacga
    14281 tcaatggcaa acaagcagta gtaatcgcta tggaaggaaa attccaaggt ggcggtatcg
    14341 gtgaagtttc cggtgcaaaa atcgttgctg cacttgaaca tgtgttagaa gaaaataaaa
    14401 aaggaaatga gatctttcca gtcattgtac tagatacagg tggtgttcgt ttgcaagaag
    14461 caaactacgg actgttatct atttcagaaa tcggaaatgt aatcattgct ctaaaacaat
    14521 atgtaccagt catcggtttg attccaggac gtgtaggatc atttggcggt atgtcgatta
    14581 cttcagcatt aatgagttat ttaatcgcaa caaaaaaagc gagagtcggt ttgaatggac
    14641 cagaagttat tgaacaagaa gcaggcgttc gagaatttga ttctagtgac aaagacttga
    14701 tttggaatac attaggatct cgtcaacggg tagaatcaaa aatcattgat gaattagtga
    14761 ctgatagtgt agaagcaatc aaagctgctg tcatcgcagc aatggacgaa cgaaaagaca
    14821 cgcatcgcag tgaaagctca gcattttatt tatcattact tgattcttta gatctttcaa
    14881 aaccaatggc cattgacgat tacaatgaag caattgctaa ccatcaagca aaatcaattg
    14941 acttaccaga agtagcagaa ggaaatgaac cagctgcagc aagtgttggt tacgattggt
    15001 ttgcggcact aactggcatg aaaaatccaa cttcttctat tagtactgtg tattatggta
    15061 aatcaagtaa attcatagaa gaagccatcg tgacatcaat cgtacctgat cctaaaaacc
    15121 cgatgtaccg tgtacgaaac ggtgaagttg gtttagttga aggtttccgc atgggacaaa
    15181 ttttggatca cgtttacgaa gaagataaag acaaaaaagt gaaacggcca atcatcgttg
    15241 tgatcgatgt accaagccaa gcatacggct ataacgaaga attgatcggt atccatgtgg
    15301 ctttggctaa tagtgctgct gcttatgcaa aactccgtca agctggacat ccagttattg
    15361 gattgattgc aggaaatgct atttcaggtg catttttagc acatggatta caatcaagcc
    15421 gcttgattgc tttaaacaat aaagaaatta ccgtccaagc gatgtctaaa gaaagtgctg
    15481 cacgtgtgac aaaacgttcg gttgctgaaa tcgaagcagc tgctgaaaaa gttccagcta
    15541 tcgcttacga tatcaataat tatacaaaac taggagcgat ctatcatttc ctagaagata
    15601 tcaccgacca agctgcttca gaacaaaatg tcagcaaagt catcgaagca atcaatgaag
    15661 ccattcaaga tgtccgcaat acaaatgaca cgatgctgac aaatcgatac acaaacgaaa
    15721 cagccaaaac attcggacgt gtcgaaacaa ataaagtccg tgccaaaatg gacgaagaat
    15781 gggatgcata aaaaatgatt caagcacatg atattgtcct ttttgataaa catgaaatag
    15841 aagggttcac cttgcctgaa tgggtcgttc taactgaaaa gatgttcgga acagttcgtc
    15901 gtgcgtcgac ggatcacgca gaaatactac cgattggttt acgtggacct gcccgcaatc
    15961 agagatggtc aacagaaatc tcaaaacatg ccgtcaaaca agtcattcat ccgtgggaca
    16021 tcatcgaaca agaatcgtat agacaagaga aaatcatgga ttatcctgtt tatcagcaat
    16081 ttcatgctgc tagaaacata ttatcaggat gtaaatgggg aatcggcggc agcttaggat
    16141 ttgaattatc gactgggatt ccggcagtaa aagaaaccag tgattttgat ttgctgctgt
    16201 acgcaaattc accgattgaa ttgccaattc aggtaattca atcgcatcct gctttttttg
    16261 agcaattcga tacccaagta gtgactcggc aaggcggttt ttcactaaaa gagtatttga
    16321 gagagccaga gaaaaaaatt ctattgaaaa cagtcgaagg acctaaatta accaaagaac
    16381 tttggtaata aagaaaaaga gagagaaaga gatgtttgaa aacaaaaaaa tcaaaatcaa
    16441 cgtaagtgac ttccagaaag tatcagctgt aacggctgtt atgatgcaaa cgatattgag
    16501 ctttgctttg gcaaacagcc acagcgcaac aaatacagct tttcttggta ccttttacgt
    16561 catcgttaaa tacacagcac caatgtttat ttttgcaatt gtttataata tggtaaaaac
    16621 aagtcagcat ttgtcttatt gggaattttt gaaagaaaag ttttttgaac tagtcgttcc
    16681 gtatcttcta tggacgacgg cttatctttg gctatttcca gcagtccaac aacaaactcc
    16741 gtatacggat actgcaagct ttttactgaa atatgtcaca ggtgatggag cgcctcattt
    16801 atggtatacc gttatgatgc tgcagatcca attgttcatg ccgttttttg tttggctcgg
    16861 ttacaaagta tttgctaata aaaaatatgt atggccagta ttgattggtt caacgatttt
    16921 atatgtcgca tggtatctgt tctatcaaac acaagtattg actgggcctt atcatgacag
    16981 ctggtactta ttagatcggt ttgttgctag tttcatgatt tatggggtag ccgcatttgt
    17041 ttatcacgaa aaagtctatc aatatttgga cagagtacgt tatcttttct tacctgttgc
    17101 cttagttatc gcgttctttt ctgtccgttc attgctcgca catccaggag acttgagctt
    17161 tgcgaatgca ccttacttga atacgatcca aagtttatac agtttagtca tcatctttgc
    17221 tgtcttcata ggcgcttcca aaatgattgt aaacgattcg ccaaaattgc cattgttcaa
    17281 atggttgtct gtttatgctt atcgtacgta tttagccaat gtatttgttt tccaagtact
    17341 gttattattg tttaaagata gttggctgca gttaccttca ggagtcatga ttcttgtagc
    17401 ttatctaatg acggcaagtt gtgcatttgc tttaagctgg ttacttcata tcatttgggt
    17461 agctatcaaa aaaggtttca gcaagtaata gtaggaagaa ggagtgagtg tcatgataaa
    17521 tgacgaacga aaaaaagcag tgactcagca actcgctgcg ttggtagagg aagcattgat
    17581 cgcagaagta acattaagtc caaaacctgg attagtggat gcactggatg cgggtgcaca
    17641 ttccgatatg gactatgctt tatttcttaa gtctgctaag acgctaactc ctttttttga
    17701 agaaatggct caaatagcat ggcatcatgc tattgatcag gagttaagag aaagaattgc
    17761 tgagattggg cgaaaagcgg aaaaggcaat gctgattgct acaaatggca tcaataccca
    17821 taaaggagcg atttgggcaa tggggctatt gatcagtgtc attgcacaaa aaatcagtat
    17881 ggaacagcct ttttctttat cgaaaatact tgatgatgtc agcaagcttg ctgcttttcc
    17941 tgatacaaaa tatcgtcata aaaaaaccac tcatggtcat agggtgaagc aaaaatatgg
    18001 cgtaaatggt gcatatgaag aagcgatatt agggtatccg catatcaggc ttgcaattca
    18061 agcagctaag gagctagata atgattcata tgtacagaaa caaatgcata tgttgttagt
    18121 tttgatgagt tcgctagatg acacatgcgt attgcatcgc agtgatcaga aaacgttagt
    18181 ggaaatgcaa aaacttgctc atgctgctag tcaaagtgct ttaccaaatc gtccatttac
    18241 agcactgttg cacttttgtc aggaaaacca aatctctcct ggaggaagcg cagatctctt
    18301 gtctgcaagc ttgttcatgc tgaagataga ggaattatgg actgccgaca catacatttg
    18361 tccgatgtta ctcaatgact aaataagaca aaattgctaa aaaaacagtt tatgcatctg
    18421 gttattgact cttcctttta gcaaggctat cataaaaata gttttttgta ataaaagata
    18481 acgaggaaga caatgaaaaa aatcaattat ttagcaatca ttttgttagt gggcctatgt
    18541 ttgtttgctc tgccgaccca aggattcgct gtcgcgggtg gtcatggtgg aggtacatct
    18601 agcggtcgaa gttcgggcag aggttcattt ggtggtcgtt catatggggg cagttatttt
    18661 ggcagtcgtt cttacggcag agattcttat gggtattatg gtccaggtac aggtccttct
    18721 agttatgagc aaacgggatt tcttgcatta gctgttttgt cagtaggagg ggtttctgct
    18781 ttttctatcg tcaagaataa aagacaaaag aaaaggctga aagcggagat attgtcccgc
    18841 atgtcaggaa cggctaaaga aaagaaagca cagtttctgg aaatccaaaa gatattcatg
    18901 aagatccaat ccgcttggga agaagaaact cctgaaaaag ccagaagctg ttatacagac
    18961 aagctgtttg ccgaacatca gaaagttctg caaaaaaaca aagcagacgg tgtaagaaat
    19021 catactaaaa aaataactat tcaggaagta ggaaattatc gacagatcca cgaaaatagt
    19081 tttgccatcc gaattgattt cagatgctgt gattattcag tcgatcgaaa agatgggcga
    19141 ttgatctcag gaagtaagac acaacgacag tatttttctc agatatggta ttttgatttt
    19201 tctgagaaag aaaaaagatg gcaagccgat tttattcagc cgattatgct ggattgacag
    19261 gaaatattta tgaacggagt tgtgacaaaa gccttgtcgc agctcctttt tctgaataaa
    19321 cggtgtgcca gagatagcct atcggcaata aggcacgatt ttgtaaaata tgggaagcga
    19381 tttttcaaaa tcgcttctta tcgctcaagg ctgaacatct ctgtcacaac ctctccacta
    19441 aatggtgttt aaaagcttgc atctgcgata ataagaacaa ccaaacgaaa aataggaagc
    19501 actattttag tttagttgtt cttattactt gatgcaggac agctttttca caacctcttt
    19561 tttgtataga tgggcaaaat ttttgtaata ataggcgtat gtctttttcg ttgaggagat
    19621 gaaaaagcat atggcttatt ttttgtttgc tttgcttact ttctttccac ttcaagtggc
    19681 agaaatatct actttttatt atgtaagcgg aaccatatac ttgaaatcag aaggagatca
    19741 tttctatgaa aacaagaaca attgaaatac ttcacagaat catgacagcc caatatccag
    19801 taaaagcaaa acatctagca agtgagttca atgtcagtca gcggacgatc cgtcaggaag
    19861 ttcttgaagc gaatatctgg ttgagcagta aaaagcttcc ggagatccgt acaattcgca
    19921 acaaaggttt tttgttgaag ctggaaaagg aaaagcaagc tcgattagaa actgaaataa
    19981 aagatgtgaa agacggcttt ttgaatcggc gcgaacgtat ttttgatcta gttttagccg
    20041 ttgcttacag ccgaatcccg atccatctaa acaaaaaaga agaagaattt caaatctcta
    20101 aaagtacatt agatgaagat atgagacggt tacgagcaac actggtcagt tatgggatag
    20161 agatcgtcag ttatggaaag caaggactgg tttataaagg ttcagaacga tctatccgta
    20221 cgatgatcta tgattttatc aatcagaatc tgggaatgac cgacttcttt atgaatcaag
    20281 atgcacaatt aacaccggct caaaaaatct ttctccatta ttttcctttg gtggaaatca
    20341 aaaaaataca ggagatctat tccaataagt tgttgaaaca agaagatgat atttataaaa
    20401 aacaaatcct gctttttaca ttgatctgga tccagcgaat caggaaacat gaatttatct
    20461 ctgccattaa ttggaaaaat acagaaataa aagacagtag cttttctgca tttattgacc
    20521 acatgatcga ggaatttcaa ttggacaatg tcccgcaagt agaaagaaat tatcttcgtt
    20581 tcactattga gacgttcaat gctagagata tctccaattc aatcgagtgg gtacaagctc
    20641 aattactaac gatccaattg atccaatttg tagagaacga gacacacgta cctttccatc
    20701 taaaggaaga agctttattc gaaaatctct ataagcacat ggctgcactg attgtaagaa
    20761 tcaagaatca tatccaagtc atgaatccat tgaaagaaaa catccgaaca aattatttcc
    20821 caatctaccg cgcagtcact cattttgttc ctacgatcga agaagtaatt ggtggcaaga
    20881 tactggagga cgagatagct tttcttgtca tccacttttc gacgatcgcc agtgtaatca
    20941 agcgagactt gaattatgtt tataaatcag tcgttgtctg taaccatggg atggctactg
    21001 gaaatctgtt agcagagaat ctgaaagaaa agtttcctca gatcgagatt actgcagttt
    21061 taagttcaaa agaaatcgaa ttagtagata aattggatgt caatctggtt ttcagcacct
    21121 ttcaattgaa ttatcgaaat aagccgcttt tagttgtcga tccgatttta actgagacga
    21181 atatcttggt tatagaagac tttttgaaaa gaaatgaaga actacagcgg ttagagccta
    21241 acagtcatga atcgacggag ttattcactc aaataataga tgtcatgaaa gaaagtggtg
    21301 gccgagtaga tcgaatcatc tatggcaagt tagaaaaatt attcgaaaca aacaatttag
    21361 aaatcaacaa aagggagatc cagcctatgt taaaagatat tttgacggac agcaatatca
    21421 tgatcaaaga aaaagccact acttgggagg aatcgattga actagctgct agaccattgg
    21481 ttaaagaaaa cattgtcgaa gagcgctatg tcgaagcgat gattcgtgca gtgaatgatt
    21541 acggaccgta tatcgtgatt ggcaaacaca tggcactagc ccatgcccga ccagaagatg
    21601 gggtcaataa attaggagtc agcgtagcta cgattgagca accaatcgat ttcggtaatc
    21661 ctgaaatgga tccagtaaag attatttttt gtttggcagc agtagactcc tactcgcatc
    21721 tgaacatcat gaaagaacta attgaattga tcaacaacga agagaagttg gatcgattga
    21781 ttgaatgcac aaatatcgaa gatttcaaac aactgctttt ttcagtagtt gagaaataat
    21841 aaatgatacg ggggagaaca aagatgaaca aattgaacat tttattcgta tgcggtgcag
    21901 gtttaggaag tagttttgca gcacagatgt ctgctgagga tgttttgaac aaattaggag
    21961 tagaagccaa actggaccat tgtgatattt cttccgcagt ttccatgaaa ccagatgtga
    22021 tcatcacagc agaaaatttt cgttcgcagt tcgaaaagtt cgcagttgat cctaaaacgg
    22081 cgatgatcta tttgaaaaat attgtttcaa aaaatgaaat tgaagaaaaa atcaccccag
    22141 ttctgcaaga taagggagtt atctaaatac aaaaagaaaa gagggtaaaa a
//
LOCUS       DAZBSA010000044        21743 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000044 DAZBSA010000000
VERSION     DAZBSA010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.044"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(17..1381)
                     /locus_tag="ACHGMT_001522"
     CDS             complement(17..1381)
                     /locus_tag="ACHGMT_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFN6835268.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(1378..2937)
                     /locus_tag="ACHGMT_001523"
     CDS             complement(1378..2937)
                     /locus_tag="ACHGMT_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6835269.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(2993..5473)
                     /locus_tag="ACHGMT_001524"
     CDS             complement(2993..5473)
                     /locus_tag="ACHGMT_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835270.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(5457..5792)
                     /locus_tag="ACHGMT_001525"
     CDS             complement(5457..5792)
                     /locus_tag="ACHGMT_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835271.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(5806..6840)
                     /locus_tag="ACHGMT_001526"
     CDS             complement(5806..6840)
                     /locus_tag="ACHGMT_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFN6835272.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(6930..7610)
                     /locus_tag="ACHGMT_001527"
     CDS             complement(6930..7610)
                     /locus_tag="ACHGMT_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFN6835273.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(7894..8997)
                     /locus_tag="ACHGMT_001528"
     CDS             complement(7894..8997)
                     /locus_tag="ACHGMT_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFN6835274.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMTPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(9219..9689)
                     /locus_tag="ACHGMT_001529"
     CDS             complement(9219..9689)
                     /locus_tag="ACHGMT_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFN6835275.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            10022..11662
                     /locus_tag="ACHGMT_001530"
     CDS             10022..11662
                     /locus_tag="ACHGMT_001530"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFN6835276.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            11816..13138
                     /locus_tag="ACHGMT_001531"
     CDS             11816..13138
                     /locus_tag="ACHGMT_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFN6835277.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(13200..13631)
                     /locus_tag="ACHGMT_001532"
     CDS             complement(13200..13631)
                     /locus_tag="ACHGMT_001532"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6835278.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(13695..14531)
                     /locus_tag="ACHGMT_001533"
     CDS             complement(13695..14531)
                     /locus_tag="ACHGMT_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFN6835279.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLINLYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            complement(15100..15405)
                     /locus_tag="ACHGMT_001534"
     CDS             complement(15100..15405)
                     /locus_tag="ACHGMT_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFN6835280.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(15430..15861)
                     /gene="ruvX"
                     /locus_tag="ACHGMT_001535"
     CDS             complement(15430..15861)
                     /gene="ruvX"
                     /locus_tag="ACHGMT_001535"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFN6835281.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(15858..16124)
                     /locus_tag="ACHGMT_001536"
     CDS             complement(15858..16124)
                     /locus_tag="ACHGMT_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFN6835282.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(16379..17104)
                     /locus_tag="ACHGMT_001537"
     CDS             complement(16379..17104)
                     /locus_tag="ACHGMT_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFN6835283.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(17465..17535)
                     /locus_tag="ACHGMT_001538"
     tRNA            complement(17465..17535)
                     /locus_tag="ACHGMT_001538"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17501..17503),aa:Gly,seq:ccc)
     gene            17652..18404
                     /locus_tag="ACHGMT_001539"
     CDS             17652..18404
                     /locus_tag="ACHGMT_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFN6835284.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            18420..18899
                     /locus_tag="ACHGMT_001540"
     CDS             18420..18899
                     /locus_tag="ACHGMT_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFN6835285.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(19150..20730)
                     /locus_tag="ACHGMT_001541"
     CDS             complement(19150..20730)
                     /locus_tag="ACHGMT_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFN6835286.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(20723..21466)
                     /locus_tag="ACHGMT_001542"
     CDS             complement(20723..21466)
                     /locus_tag="ACHGMT_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6835287.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(21642..>21743)
                     /locus_tag="ACHGMT_001543"
     CDS             complement(21642..>21743)
                     /locus_tag="ACHGMT_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HFN6835288.1"
                     /translation="IVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
BASE COUNT         6410 a         4366 c         3891 g         7076 t
ORIGIN      
        1 aagaccgtga tatttctcac agtcttttat ctactctatt gctatttgct ctcttagcta
       61 gtactttaaa agctaactca ttgaggtagt gaaaacgaga ggtgtattca gacagaaatc
      121 caaaatcctc aactgctagc tgtggaatcg tagttttgtg tttcacgata tccgcaagca
      181 ggttcaaatc tgataataag aattcttggc ttatcagctg tccgccaatt atttctaatg
      241 tatccggatg gtagatcagc tttgcattta ctgttttgtt ttcttcgtcg tattgtggaa
      301 atagatcaaa ttctcctgaa cacgaaccgg tattttgttc caagaatggc gcttcatctt
      361 ctgtcagccc tacagttcct aaaaagtagt ctataatgac tgctgctgtt atcttttgtc
      421 tgaggtcgta agcggttggc tgaccactca aagttagcgc tactgctctt gccatatgta
      481 cagctcgagt aactaaaggt aaaaagagat gccgtttatt tttgtcaaaa ctgactggga
      541 cgatatctcc aatcgcatag atgtccggat cacttgtctg cagatattca ttgactatca
      601 cagtctcatc ctcatacaaa gccacttttt cttttaacaa tgtcgtatta ggacgagttt
      661 gggcagaaaa aatgaccata tctgccttta ttgtttgaga cagaagttgt acggatacta
      721 ctttctcttc tgattttttc caatcgatca agaattcatt tggataaaaa tttatttgct
      781 tagtttgcag ctgattttga aaactgttga tcatatcctt atcgaaatat cttgataaaa
      841 cattcggcat ataatcaact agatgaataa tgtatccttt atttctcaaa gcatctgcta
      901 attccaatcc aatgtaacca gcaccgaaga tcactacttc ttttgcttct tttaaagcgg
      961 caagtacttg cttagattgc actagatttt tataggtgta gacattttca gcttcaattt
     1021 gtttatacaa ggtgacagga gaggaacctg tcgctacgat taatttatca taggttatgg
     1081 tctcgttttt tccttctttg gatgcgagga tgagctgatg tttgtctgga tcaagttcag
     1141 ttactctcca ttcggtgatc agttttgccc cactttcttg aagagtccgc atgttatggg
     1201 caacttcagc taaactacgt atctttcctt ttaaataaag gttgattcca tttgaaacat
     1261 agcctagtcc atctttatga tcttcatcga tcagtaaaac ttctgctttc ggttgaagtt
     1321 ttcttagatt tagggcagct gtgatgcctt catgggaagc accaatgatc acgattttca
     1381 tgttttattc atccttaaat tcaatttttc taaataacat tcttttaaaa attggttcaa
     1441 ataggggaaa tcataattat attcgttgct agtgaataca aaaactttag cggacttctg
     1501 tgacgggtac tctttcaatt ggctagtcaa aatgatatca tactgctttt ctttctcgta
     1561 ttcgactaaa tcatatttga tcgtattcag caattgttcc tggatgattt ctttcacgat
     1621 atccgccatt agcctttcat aaggaagatc ataagcaatt tgcagacgta attctaactg
     1681 cttgatcagc gtccgtaaaa tagatgcata gcgagcaaat aattcaaagc gggcattttc
     1741 ctcgtctact tcagtaaaat caacaatagc aaatgtttgt ctgaccaatc tttcacataa
     1801 aaccaggaat tgtttatctt ctacttcagt taaccgctgt tttaatcctt cttggccaaa
     1861 aatattcatc catcctctaa aataggcaaa atgagcgtga ttttgcatca ctgtcaacgc
     1921 aatcttttgc gaaatctctt ctggaaaaac ataaaaagag aaatattctt ctatcgattg
     1981 tttaaacgtt cgattcaatt gagctactac gtcgtctttg tatttttcgc ttatcaaaaa
     2041 ctcctggaca tgcgagttat gtgtatctaa tgtaaagtta ctgatcatga aaagataaaa
     2101 aaccaacgtt tcattttgat tccaaggaaa ggcatattga aagaagtaac gggtcaaaaa
     2161 tgtatggagg tccaataaaa attgtgtcgt ttgaaacaat ccaacaggaa gattgctata
     2221 atctggtttg gattttttcg tacagcgcat tcgcttcttc ataatggaaa gccatatttc
     2281 aaatttcaat cgtccttctt ctgtaatagc aaaactaaac tctttttgca gcagatctaa
     2341 aaaagtgttt cctgataaat cactgtattc ttttttgttt tcaagatagg gacgtccaaa
     2401 ccaaaataat tggaaataaa aataacgaat ttgactttct ggaccggtca gccgcccgct
     2461 ttttattttc agatgaaact catttaaaga atcatttaat tccttcactc tgcgagacaa
     2521 agtagccgaa ctgatattaa gttccatgac aagtttctgc cgatcaaatg agccattttc
     2581 caacagataa agaagtatct gatattttgt agaatagcgc aagtaacaat gataataaac
     2641 atcgactgct gctatatttc catgtctgat taataaaata ttcccttctt ggataacaat
     2701 ttccaattgt ttgatatttt ccaagttagc ttgtaaataa aacagatctt ctttcaaggt
     2761 agcccgatca actgataagc gggtaagtac gttattaacg gaaacctctc ctttttcctc
     2821 taataaaagt tggaaaagtt tgatttgccg ttgttcattt ttttcaagca aactgaaaag
     2881 ttccaagtcc actccttcaa gcatttctga ttttaacagc agaagtttcc ttctcatcct
     2941 tgaaaaagag gatctgtgac agttatcaca gatccataca aattctaatc acttagctgg
     3001 ttggcccagc tgtcagttcc catgtgacca tgccttgata cgttgcgcca ggttgagcat
     3061 tcgtactgct atttacctct aagagtaaac catcggtatc gggaaagtca acgagaacct
     3121 ctcctttagc tgttccatca ctctcataaa tcaacgtgtt ttgaccttgc tgaatcaatt
     3181 gctttgatcc agaatgattg aagtataatt ttgttccgct taattcttga ccttgatcat
     3241 tcttaaataa agaagtcaat gtacctgtca gttgccaagg agacgtgctt ggaagccgag
     3301 tatcccgcac atcaaaagaa aaatcgcctt ttgattcata ataacttgtc ttcaacggtt
     3361 tggggagatc gttaccaaat gtaaaatcat ctggtacatt aactaatgtt aattcttgtt
     3421 tggggacaag aattgtttta gcagatgttt ggacaactgg gttaggaagc ttagcaccat
     3481 ctgctaatgt aatattgctg gaaagtacag ctgcctttgt cagtatgctg acggattgta
     3541 aagaagtatc tacagtattt aattgatagt tgtaagtaag atgaatggta tctcctattt
     3601 tgctcaatag atctgctggg acagaaactc cttgttgaat gccggtatct gcgatagttg
     3661 taccatttaa ttttaaggaa ccattgatat aagtagtatt ttcaggaagt gagatcgtga
     3721 tttttttgtc ggttatcgca tccccagatt ttgttaacgt gaatgtcaca ttttcttgaa
     3781 tcgtatctcc tgatagagca gacaggggat ctcctgtctc aggttgtctg ctaaggtttt
     3841 ttagctgact atctgcactc acagatggaa aattagccac tgttatttga ggaagagtgg
     3901 tgattccagc ttgttcagac tgccatttgt acgcaaaaga cggatacatg ttagtaagta
     3961 catcgtccac caagcgataa tcaaaataat attgccctga agccaatgca ggtagtgtag
     4021 aagtagctgt atttacaccc ccgttatttg attggatcgt ttggaaaagt tgatctgtcc
     4081 atcctgtcgc taatttttgg gttcctttcg aataaagcct gtaatggatc gaaccctttt
     4141 ttagttcagt tgctgaccaa ttggccgtga cttggtacga tgcagaagaa aggagctttc
     4201 cccactgact atcggctgtt aagtttgttg gtaaaaccgt tccgtctagc ttgtactgct
     4261 ccaatacctt taaagcaaca ggagaaagca atccgccttg cgcatctttc ccactgtaca
     4321 cgatattggc tgtctgattc aatccacttg tgcttgctgg agtatgagtc agtattgctc
     4381 cttgtgtaac gttttttcct gtaaaatagt tgactacgcg atcaaagcga ctaaatggca
     4441 actgatcaaa acgtccttca gtcagcccca tcgtcaatgt ccgactattg gtagagcctg
     4501 gaaggttctg gttatttcca tccacgtatt ctgtggtatc agcaaatgta ttatattttc
     4561 ctgttgaatc acttggtgag atgtaactat tccacgtatt tatctctgca cttgcagaaa
     4621 atgcatatgc tcctggattc aagtttccgc cagttaattt aggataaaat ctacctgcag
     4681 ctggagctaa aatcgaattg aacatacgat cttggataat attattcgaa tcataaaaat
     4741 caacagaaaa aacggccttt ttagacatag aaggttgtaa tttacccttg gtcaaaattt
     4801 tgtttggact cgtatcatat ttatctccgt tttctgtcat cttcgcgaca ttagctgtaa
     4861 catcaaattg aagcgggata tcgtccacgt tgaattcgcc tcgcgtaata atagcaataa
     4921 tatctgtcaa tagagtattt gtatcccacg accccatccc aaaacgcaag gtatggcttc
     4981 ctgctgataa taaacttccg ctagtaacct tgatgggggc accggcaata tacaagtagg
     5041 cgttgttcca gtcaattgct tttaatacat cactgcttga tacatgttcc ggcaaattga
     5101 tgatcgccac aaatgcaaat ccttgtgcag tgatattcac caaatcaagt acatttgtta
     5161 gtttcacatg gctttcccaa tgaaaacgga ccgtgccacc tttccaagga tcatttgaac
     5221 catcgttcaa ataagtagca gtcaatttag gcaattggtt agccccagtt agttggacaa
     5281 gcttcccgcc attgactgca ctacgtccac catttggagg tacgatattt tcatacgtgt
     5341 atggatcata agaaatggca ggaggcgttg ctccaacagc ttctgccgaa ggtgaaaaga
     5401 taaatatact taaaacaaaa agcgaagtaa aaaagaagga gcgaaaaaat gaaaatttat
     5461 tttgtagcaa catgttcatc ctccttttcc tttcttctcc ataacaacaa ccacgcaaga
     5521 agccataatt ctataccaat gatcaaataa agaaaacgga tcttttgttc attcgtctcc
     5581 gggaatacgc catgttgatt agattcttta taaatcggta gctgtgtctg ctgttttgat
     5641 gcgccattga ctgaaccact tggatgcgga attggactta gatcataaaa tgtaatcgta
     5701 cctgaacttt tggtatctgc ttggactggc tcttgccagc tgaagaacag caaacttgca
     5761 ataatccctc ccaataagat cgatgttttc aacgaaatca cctcatcatg ttgtttctcc
     5821 ttttttcttt ttcttgtgga cccacaaaaa cagcaaaatc aaccataaca ctagaactgc
     5881 agcaaccaat cctgcataaa gccatgggaa agattgttct tgtactgcaa aaacggaatt
     5941 ttcatttact ttttgtgctt tttctttttt gatcgtaaag ttttgtgtaa atcgccattc
     6001 attactgtcg cctgattttg ctatgatttc tgcgcggtaa tcgccggctt ttgctttatc
     6061 accagctaaa tcaagattca agtgaaaatt cgaatttggt gcaactaaaa atgcattaga
     6121 cgtattttca atataaagct gattttcata aaagatcttg ctcgtaaata tcaaattgct
     6181 gataatccta ggctgaggat tttggaaacg aagctgaaca gttggctttg cgttgacctc
     6241 gtccaaatct cctgataaaa gtttcagatc cggttctatc gtctcttgac tttcttgtaa
     6301 aacgactgcg agattataag caatacgatt tttgatttgt gctccttctg tagcattttc
     6361 tgtttcagct tcttctttag gtgaaacatg aatacccgct aatacacgtc ccttaaagct
     6421 ttttgctggg attttcactt ctataggtac agttgtttct gcatgtgctg atacattgat
     6481 ttcctttttc gcaatgagca aaacgtcacg gacatcaaac ggcaatgtct catctaacgt
     6541 tgggtttttt tgactataat cgatcgttcc accatcactc gttaccgccg gattgacaga
     6601 gacctcaaat gttcgttctt catctgaaga attgctgata attacttcta gtgtctgatt
     6661 ttgttccggt gccactaata aatcaaaata accgattgct ttatctattt ggttctctgg
     6721 aaataccggt ttgattccaa agtctcctgc tgaatccttt gcttggctga ttgacggaaa
     6781 aatgaaaaat agacttaaaa tagaaaaaaa cgaaacaaac agtcgtttac tttgattcat
     6841 tgtccaccac atcctttcaa caaaaactgc ggtaaaaaat ccttcaccta tagtattttc
     6901 accgcagttt gttgaattta tttcatactc tatgctactg ggccagccac gaggttccaa
     6961 gtcaaatcag ccgtgaatgt tccagctgct gctttcggca ctttcagtgt gatatttgct
     7021 ggatcataaa cagaaagcca tgttcccaga ccttgatctt ttgctgctgt aaatattggt
     7081 gtcgctgcac ttgctttact caacccagtt acagtttgac tagttggtcc agtggattcg
     7141 ttatctgacg tacttttaac agtggatgtt ccaaaatcta attcagcgcc ttgcaaaact
     7201 tgtgaaccgt ttacaaaatc tgaaaccgaa actgtcacgt tccacccttg tgcttggcca
     7261 tctgcaccta cacctcgacg atctgatact tggagataag gtgtatcgtt ttttgaagcg
     7321 tctacttgat acgtttttgt tccgctttca atctcgtgtg taccaaatgg caattcggga
     7381 actacatcca gtgttaaagg acctgtctgg ccagttgctg gattttccgg atctgctggc
     7441 tctactggtt ttgaaggatc agtagggtta agaggctcca cggtatttgt tggttcagta
     7501 aaggtaatat ctccttttga agtcgcttga ccctcagcaa aagctggtgt tgccaagccg
     7561 cctagaagaa gtgttgaaag taataatgtc ccaatcatgt tctttttcat aataaatcac
     7621 tcctttttct tagtgaccta agtatagtag agacagtatg caaaaaatcc tcatgaattc
     7681 atccgcttac ttgaaattat gacccttttt gtataaaaga gaaaaaaaca ataaaatcgt
     7741 ttacatgaat attcttcctt ttttattaag tatgaaaaaa tatttcaatt ttattttttc
     7801 gtttcaaatt attctattag tgacatcctt gaactataaa aataggagta cggcatcatc
     7861 tgccgcactc ctattttttt cgtcatttgt ttcttaaata aaacgattct cacgcaattt
     7921 tatttttttg atatcttcta ctgtttgtgt tccagctaat tgcatgacca tttccaattc
     7981 tgttttaaag aaatcaaaca cttgatggac tcctgtactt cctcctaaag acaatccata
     8041 aataacgggt ctgccaatag caacaagatc tgcacctgat gcgattgctt tgaaaacatg
     8101 ctgtccgcgt cgtacaccgc tgtcaaacac gattggtaca cgtttatcta ctgcttctgc
     8161 cacatattgc aacgaatcaa acgctgctgg accaccatct agttgacggc caccatgatt
     8221 agatacccag attccgccag caccagattc taatgaacga tagacatctt cttctgactg
     8281 gacacctttc acatagactg gcaaatcaga atgagcagca ataaattcta catcttttgg
     8341 actaagcttc tgtttggaag atttgtacac agcatccatc gtttggccta ctcctgattg
     8401 ataagcctga acgatcggca ttggcagtgg gaaggtaaac ccatttctgc ggtctgtttc
     8461 acggtttccg ccaactgttg catccgctgt caatacgatt gcttttgctc cattacgctt
     8521 ggccacttca agaatatcca aattgatccc atcatctttg ctcatataaa attgaaacca
     8581 ttgcggtgct ttctccccgc ctgcttctct tatctcttct aatgtacaag aagcgtaaga
     8641 acttgctgta tagatcgtcc caaaatctgc tacacctttt gcagaagctt tctccgcctt
     8701 cacatgagct aagccatgag cagcaactgg tgtcatgatg attggtgcag tcagcgtttc
     8761 tttgtcaaaa tgtgtagttg tatcgggtaa ttctacatct tttaatacat gaggaataat
     8821 cagctgatga ttgaatgccc gttcattttc ttgataagta aataagtcgc cagcaccact
     8881 gctgatgtaa ccataaccac cagctggaat cacttgtgaa gcggctattt ctaaatcttc
     8941 catattgata aaatccacta ttccttcatt tgtacctgct tgatacgttt tttccatttc
     9001 aatgccttcc ctctataaaa aattgaatga ataatatgat agcgtttaca cactttatat
     9061 tactcttccg tattgataca ggcaaataat ctgtttacat ttcagtcatt ccagttgtta
     9121 ctactctgaa agaaatgttt agaacacaaa tagataaaaa acgatgggac tttgacaaaa
     9181 gtcgtgtcaa agtccctttt acgaaatact gtggatgcct attgaaataa gtgcgggttg
     9241 tttctttgta tttcaatcgt ttcttgccat tcttcgttta attttggatt accgttttct
     9301 tccttgaacg cataaggtcc caatccaaaa agctcagtta gtatttgatt cacgattcca
     9361 tatagctcgg tgtgatcgga cacatgatta ttgaagatca tctcattatc accacgtttg
     9421 aatctcacat tcgggctctc atgatgaacc tctatgcagt tttccccttt ttttaactga
     9481 cggatttctg aaaacatctc ataaatatct tctccaataa aaccttcagc aagcgccaga
     9541 ctgtccacaa gaccgcctac tgtcaactca gagattatcg ctttgttggc accaagcttt
     9601 aatgtatagc ttgttgcttc tactacgccg catgcattgc aaccaccttc tgatagttcg
     9661 ctttttctga tcaattgaac ttctttcatt ttctgttcct cctcttagtt ttctacttat
     9721 gtatttttta tcacctgacg taagtgtatt ttatcgtcac gatcacgtga tttattcttc
     9781 agagattcta tttatctatc taacatatcc tttattcttc tacttttctt tctttagcat
     9841 ataagagata cgcatagtaa tcaagcaagg attgttgata taaaaagaac aaataccgct
     9901 attttgattt cttttgactc tattactcat ataaataaaa tttattatac ctgctctttt
     9961 tgactatatc ctcatctgac tcctatactt cgtttgtatt aaaacttagg aggaatcatt
    10021 catgcttaca gggctaaact tattaaataa tccttttcta aataaaggaa cagcttttac
    10081 aaaagaagaa cgggcaaaat acggaatcac tggtatgctt ccaagtactg tccaaacatt
    10141 ggaacaacaa tctgtccaag cttacggtca atacttaagc aaacagaccg atttggaaaa
    10201 acgtatcttc ttaatgaatc tattcaatac gaaccggaca ttgttttata aattgatggg
    10261 cgaacattta gttgaattca tgcctgtcgt ttatgatcct gtcgttgcgg attcaatcga
    10321 acaatacaac gaaattttct tgaaaccaca agatgctgca tttctttcaa tcgacgaacc
    10381 agaaaatatc aaagcaagtt tgaaaaacgc agcagatgga cgcgacattc gtttgatcgt
    10441 tgtgacagat gcagaaggaa tcttaggtat gggtgactgg ggtgtgaacg gtgttgacat
    10501 cgctatcggt aaattgatgg tctacactgc tgcggctggt atcaatcctg cacaagtctt
    10561 gcctgtatca atcgacgctg gtactaataa cgaagaatta ttgaataatc cattatactt
    10621 agggaatcgt catgctcgtg tagaaggcga aacttactac gaattcattg atcaattcgt
    10681 tcaatcagct actgaattat tcccagaatt gttattacat tgggaagatt ttggacgagg
    10741 aaatgctgca acgatcttag aaaaatacga agataaaatc actactttca atgatgatat
    10801 ccaaggcaca ggaatcgttg tactagctgg tgtattaggt ggattgaata tatcaggtga
    10861 gcaattgaaa gatcaaacga tcttgacatt tggtgctggt actgctggtg taggtatcgc
    10921 aaacatcctt ttagatgaaa tgatccgtca aggtgtacct gaagaagaag cacgccaaca
    10981 tttctatcaa gtagataaac agggtttatt gtttgaagat acaaaaggat tgactcctgg
    11041 tcaaatccca ttcgctcgta aacgctcaga attcactaat gctgatgaat tgacaaactt
    11101 agaagcagtt gtaaaagcaa tccatccaac gatcatgatc ggtacttcta cccaacctgg
    11161 agcattcact gaagaaatcg tgaaagaaat ggcggctcat accccacgtc caatcatcat
    11221 gcctttgtct aatccaacaa aattggcaga agcaaaagca aaagatttga ttgaatggac
    11281 ggacggaaaa gctttagttg gaacaggtat ccctgctgct gatgtggaat ataacggtgt
    11341 aacttaccaa atcggtcaag caaataatgc gttgatgtac ccaggtttag ggcttggttt
    11401 gattgcttca acagctacac gagtaaatgc tgaaatcatt tctcaagcta gtcgagcatt
    11461 aggcggaatc gttgatgtaa caaaaccagg agcagctatc ttgcctccag tagcaaaaat
    11521 cactgaattc tctcaaacga ttgctgaaac tgtcgcaaaa tcagttgttg cacaaaaatt
    11581 gaatcgtgaa gaaatcacgg atatcaaaga agctgttgaa tctgctaaat gggttcctga
    11641 atataaatct ttagaagatt aagattgtta tagtaaaaga acatacagaa actttgtatg
    11701 acgtctaaaa agaaaaaagc gactgcggca aaacagcagc cagccaacta ctgctctttt
    11761 gccgcttctc tttggctttt ttcatacgat acagaataag aaaggatctg taaaaatggc
    11821 acaaaaaaca acaaccgcaa ctgcagttga ggaaaagaaa aaagaagggt tttggtcaac
    11881 aaaaattacc ggcgtaagtt tgccccttta tttgatcatg gtccttgtcc tgatcattgc
    11941 aatggcttta ggaaaattac cgacgaatat gattggccca atcgctgttt tggtcatttt
    12001 cggtaattta tttcatttta tcggaacaaa aatccctatc gtcaaaagtt atttaggtgg
    12061 tggctccgtt tttgcgatct ttgcctctgc cgcgattgct acatttggga tcttacctga
    12121 gtatgtagta aaatccacag aaaactttgt cagcaatatg ggctttttag acttttatat
    12181 tgctgccttg attactggta gcatccttgg tatgaaccgt aatctattga tgaaagcttc
    12241 tgttcgtttt attcccgttg cacttatttc aatggtcgtc tccttttttg ccgtaggact
    12301 cgtaggtatg cttatcggaa atggttttgc aaattccgtt ttatatatct cattgcccgc
    12361 aatggctggt ggtgttggag ccggagctgt acctttatca acgatttatg caaatgtttt
    12421 aggtacggac gcttcttcta tcatttcccg cctgattcct gcgacagcaa tgacaaatgt
    12481 cctagctatc attggtgcag cgttagttgc tcgtgcggga caatcaatgc caaaattcaa
    12541 cggtaatggt aaattaatgg aaaaaggcga ccttggcgat ggaaaaccta gaaatgtaaa
    12601 accttccatt ccacaattag gtgtcggttt gatggtatca ttgactttct tcatcttagg
    12661 acaaatctgt aactattttg ttcctaaagt ccatgcttac gcattcatga tcattatcgt
    12721 tgttatctgt aaaatcgcaa acctattacc agaatattac gaagatgcag ctatcatgtt
    12781 caacaacttg attgtcaaaa acttgacagc agctgttctt gccggtatcg gtattgcgtt
    12841 attgaatcta aatgtattgg cttctgcttt gacatggcaa tttgttgtct tatgtttgac
    12901 aagtgttatc gcgatttcag tagtttcagg atttgtcggt cgtttgtttg gtttatatcc
    12961 aatcgaatca actattactg ctggcttatg caacaactcg atgggtggta caggaaatgt
    13021 agctgtctta tctgcagcta atcggatgga acttattgct tttgctcaaa tgggcaatcg
    13081 gcttggtggt gccatcgttt tgatcatctc aggtttcttg atgcaattac tttcttagtc
    13141 aaaaaaaata cctgatcatc aagtatcggc ttgatgacca ggtatttttt gataaaaatt
    13201 tacaggattt tcaaaaatct tttttgcatt ttatcggaat gataaccgat gcgttcataa
    13261 aatcggtgtg cttctactct gtattctgca gaattaagtc ttattgctga tattcctcgt
    13321 cttttcgctt cttctacgcc agtcatcaag gcctttccga ttcctctttt ttcagcatgt
    13381 ttagacacag caagtgccat aatattgaac atcggatcgc tgaaaatcga ttcataggac
    13441 tccgcttgta catagccagc tgctttttta gaagtaggat cttcatagac caagaaaaaa
    13501 tttttttctg gctccttcaa cagtttcttc atttgtctca ccgtaacggt taatggtaca
    13561 tcatatccta attgttccgc attgatttct cgtagtgctt ctgcatctcc taaacgcatt
    13621 tcccgcaaca tccaaatcac cagccaattt ttattttttt aaatgaattt ttttatcaca
    13681 gtatactcct tttttcattt agaacaatat gtttcagtga ttttgacttg cgttagtcta
    13741 gattctccta atgtttgtaa gatgacgaaa gcattgtaaa gattgatcaa aagaaccacg
    13801 aggacaatta cttgaaaaat aaaggtcact ttttttggag acaaaaccct gctaaatctt
    13861 gctccccaca aacctccaat aacagcagcg gggataacat aaaacaacat agataaatca
    13921 aagcgcataa agctacttgt taaagcaatc gttaccaatt ttgccagttg agaaaagaaa
    13981 atcgtagata gagaatatag tgttgcttct tttattggca atgcaaacat cagcatcaac
    14041 aaagaaacat tgactggtcc accaccaata cctaaaaaac tagctaaaaa gcccagaacc
    14101 cccccacata taaggtacca agtccatgac gtcaaatgaa attttggctg atgatgcttg
    14161 gtgtagaaga acgcaaaaac caaggtcaat accgtcaaaa aaatttggat cagctgcaca
    14221 tgcttctcgt tttcaaataa ctgtaaaaaa acttcgaata caatgttgcc gactactcct
    14281 ccaacaacag ctccactaga tacccaaccg acaatctgcc aattcagtga tttcccgctt
    14341 gatacttgtc gcacagtcga aacaatggac atcgtgaata ccgcaactgt tgagtaaaaa
    14401 gaaatccctg caacagaatg ggcgccgata agatcaagaa tcggtttgat caacacgcct
    14461 ccacccatcc cggaaatagc gccaactgtg ttcgccagaa cgatcaccac aaaataaatc
    14521 agaccaatca tcttgtattt cctttctctt gtttatatct tttagcgatt gttactcttt
    14581 tcttttatta tacagtaaaa gaaacaaacg aggcttcgaa agcttttcta ctttttaagg
    14641 aaatttagtg ctaaacgaaa agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa
    14701 cagccaaaca aaaatagctc ctcataattt tcgtttggtt gaacttatta ccgcagatgc
    14761 aagcttttga acaccgttta agaaagaggt cgtgagcctg ccgttcagct ccgagtcaca
    14821 aggaacaatt tcaaaaaaca gcttctcatg tttgttgaat attgtgactt gtgaccgagg
    14881 agttggcagg agaacaccgt ttaaggaaga gactgtgaag tcaacgtttt gacctgatta
    14941 ttagagaaac aaatggcaaa atagttcttc atatttcgcc gtttttcgag cgaatgccga
    15001 ggggcagctg attgaacatc gtttatttgg aaaaagggat tgtgacaaga cgtttgtcac
    15061 aatccctttt tttatatctt attaattcat tcttttgttc tattcttctt cttccgccat
    15121 gaatgtattg aatacttctt caatcatgtc ccattctgcg tctgtttcga tttgttctaa
    15181 ctcgccttct gttccatctt cgttttcgat gtatgcataa gcttgtaatt ctacatcttc
    15241 atcttctgaa acaccagcag gatataaaag aacatagtta cgtccaaatt cttcttgtcc
    15301 gtcaactgtt agtaggattt catataatgt ttcatttcct tggtcatcca ccaatgtgat
    15361 gtgttcgtgt ccttcatggt cgtgggaatg gttatgttct gtcataagat tacctctttt
    15421 cttttattat taaaataatt gcttacctga gccgtctaaa taattttgta agatcatcac
    15481 agctgctaat ttatcgatga ctttttttcg tttggctctt gaagtatttg cctgctctac
    15541 tagcatacgt tctgcctgaa cagtggttaa tcgttcatct tgataatcaa ctggcaagga
    15601 gaatagttct ttgatctttt ctccgtatga catggaagct tctgcccttg gtccgatgga
    15661 attgttcata tttttaggta atccaactac aaaccggtct acttcatatt gtgctactaa
    15721 ttccttcaac cgatcaaagc caaactcttc ttctgcttca ttgatccgaa tgatctctac
    15781 tccttgagct gtccagccaa aggggtcact gatagcaaca cctactgtct tagaaccgac
    15841 atccaagccc attactctca taaattcact ccatgatttg agaggtagtc cttgacaatt
    15901 acttccatga tctcatcgcg ttcgtgacgg cgaatcaaat ttcgagcatc acgataacga
    15961 ggaatgtagg acgggtctcc agaaagcaag tagccgacaa tctgattgat tggattgtag
    16021 cctttttctt ccaaagcccg ataaacaatc gctaatgttt cgctcacttc ttttttgcga
    16081 ctatcgtcaa aaccaaaacg tacggtttca tctgtaaaac ccatgattct acacctcttt
    16141 cccgcaaggt aattccagct taccttctac aaccttattt tactcgaaga aagggaaaac
    16201 tacaaacaaa tattccaaac ttccgaataa attaagaaac actatttgta tgtttttttc
    16261 cttttttctg tagctttatc aaaagtactc ttttcataaa gtatacgcta tatttctttg
    16321 aatgtatgtt tttacacaat tttttcataa tattcataaa aaattcgaat agctgctact
    16381 aattttttgt ttttgttgct tgtcctgtct cataattgat cgtgtagcca tcttgaacat
    16441 tgtagataaa gacattgaat tcaatttttt tgtcttcaat gctttctgct tccaattgca
    16501 ctcccctggc taccagttca ttcccctcaa aatgaggagt gacacggtag cgtacatgat
    16561 gatttgtttc tttgatatat gccgcaatat cattttcgtg agcaagcatg tatggagcat
    16621 tcaacgaacg tgtccctgtc atcaagtttt taatgttgtt attttgacca gtcagctgga
    16681 aaccaattaa atggctgcga ttatataacc attgcccgtc acttaatttc ttttgtttcc
    16741 aacctgtcgg gtcaatatac aaaggctctc tcttttcagt agggaacaaa tcttttccaa
    16801 gcatagcatt ggcttgtcct actctgttca atttatcaat atttgaatag ctttcccatg
    16861 ttcctttgga aaggctaaga tcctcttttg taaattccgg ctgattccca ttgatttcta
    16921 tttcttgatg ttttccatca tattctggca catccgctac ttgagaagct tgtggtgtgg
    16981 aatcaaaaat agttgtaatc tgctgaaaga tatctgaatc tttttgtgtg gtggaaacac
    17041 cgaatgcccc tgcaatcaca gcagcgacga tccccacgat cgccttggtt gtttttttgc
    17101 tcatttcttt actcctttac taacagctcc ctaaatctta acatatgttc ccctataaat
    17161 aaatactaga aagaaataga aacagcaaaa aataaaacct gttttaagat ggcttaaaaa
    17221 tacaaaaaag agacggattc ctcgtctcta atcagtcacc actctatctg taaggtattt
    17281 attattattc aaaaattttc ttcatactca agacaataca ctgatttcac aaccgcttgg
    17341 taaacggtgc gcaaaagctt gcatctgcgg taataagtcc atccaagtta aaaacataag
    17401 aagttgtttt tgcttggctg ttcttattac ttgttgcaga gcagcttttt cacaacctat
    17461 tttaagcggg tgacgggaat cgaacccgcg acatcagctt gggaagctgg gattttacca
    17521 ctaaactaca cccgcaatac aaaaacagta tacgcctctt ttattccttc ctcaagtttt
    17581 tttattcttt acgcttctgg tatgttttgt agtaaaataa aactaatcaa tcaattattg
    17641 gagaagatgc tatgacggga aaagaattag atctgttgct ggacacttgt ttactcgctg
    17701 ggaaaatcat gatggaaagc aatgcggaga tgtatcgggt agaagatacg atgagtcgaa
    17761 ttgctttagc ttctggaaat ttccgtttgg tcagctatgt cactcaaacc ggtctcttta
    17821 ttggtcttga tcgtacctcg accatccgaa tggaacaaat taccaaccgt tcgattaatt
    17881 tagaaaaagt agtaaaagta aatgatcttt cacgtaaata tgttgcttca gagctaacat
    17941 tagaagaact ttactgcgaa ctgcaagcaa tcgaccgaga ccgttctttc tttcctgtct
    18001 ggttgcaaat cgttagcgcg gcagtgatca gtggaacaat tatggtgtta tttggcggaa
    18061 agatgaccga tttacctgtg actttagtca ttggaggatt gggatacgca gcttatctat
    18121 atagtttgaa attttttaga atcaaatttt tatcggaatt tttatcttct ttattgattg
    18181 gcatagccgc tatctacagc gtacgttttg gcttaggaag caatcaggat ctaatcatta
    18241 tcggatgtgt gatgccttta gttcctggag ttcaaattac gaacgctatc cgtgatttgc
    18301 tagctggaca ttatgtctca ggagtttctc gcggaacaga agcgatgatg actgctacga
    18361 tgatcggttt tgcgattgct tttattttcc agctttttta ttaggaaagg aacacaagta
    18421 tggggacgat atttattcag ttttctttta gttttttagc gactgcggca tttgccgtta
    18481 ttacaaatgt acctaggcgt tcattgattt gttgtggtct ttctggtgct tttggctgga
    18541 tgatctactg gatctgtgtt caaataggcg gatctgctgc ttttggttca ttgcttggtt
    18601 ctttaggagt agcattcatc agcgatcttt tctctaaacg tttgaaaatg cccgtaacga
    18661 ttttcaatat tcccgggatg gttcctctag tccctggtgg tttagcttat caagcggttc
    18721 gaaatctcgt tactgaatcc tatcgagaag cggctcatta tgcggtccaa gcaattatga
    18781 ttgctggagc aatcgcttta ggtcttgtat tatcagaagt ctttaaccat aacatccgta
    18841 atttcaaagt aaagcgtgac gcaatcatga tgaaacgaaa aaaacggctt tttaaataaa
    18901 cgaagtgtaa caaagaaaag gttgtgaaag agacgttcag ccttgagcga tgacaaaata
    18961 tctcacaaac attgcgacaa gtaaccgcag gagcaataag aagtaattat gagtattaaa
    19021 aaaagttttg taaaaatagc ttctcacatt tttacataaa attgacttaa tactgagaaa
    19081 ctgacttttg aacaccctct atctgtaaaa aaggggctgc gacatgattt ttgtcacggc
    19141 ccctcttctt tattcaaatt ttttccaaaa cgttcgttga taataaacga tccatagagc
    19201 ggaaagtatt agaactaaac cgatcacagg aatattcact aaccaaaatg gataaaacaa
    19261 agccaacatt ccgtataaga cgataagaat aatactgatg atcaatgaac ccatcatagt
    19321 tgcgactaaa cccaaacttc ctgaacctct gttaaataat tggttaatat tggtccagtt
    19381 cagcagcagc aaccgataat ctctagcgaa ataacgtagt gacaataagt agcagccgaa
    19441 taaagaacca aatagcaagc tagggataaa aaacaaaggc agctgaaaag acagtccgct
    19501 taataatgcg atcccaccag ttaaaaaact ctgcaatatc catccgaatc tgaacttttc
    19561 ttttaaatat tggttcatag agattggcaa agaacggata aacagaaaat tttcttgatc
    19621 caaagaaata agattgctga taaaagaagt ctgattaact gttaagaaag ccaatgcgat
    19681 ccctaccagg aaaacaacac cgattaactt attatctaaa tcagttaatt ttatttcgcc
    19741 tcctagggca aaagcaagaa taaaaatgat tggagttaac aaggaagaag aaagaacttg
    19801 cataatcaaa ttaggttctt ttactaattg agcattgtaa ctaaataaaa gctgatgcac
    19861 attttggttc tttttatggg ttctttttat tttgaactca ctgtttgaat cagtcagttg
    19921 ctctgtaagc tttggtacta tcagctgttt tacagcaagg aaaaatccca gagccgtgat
    19981 agctagtatt ccccaaccca ataaaccttg ttgagaaagt ggctgactgg cagcatcata
    20041 gaatggcaat aatatcgaaa tcggatgccg atctgttaac tcattttcaa tagtccccgt
    20101 ttgatgattc atccacatga ttccagcaaa tgcgatcagc atcgagctgc caagcaagat
    20161 actagaaact gtttttctat gttttttaaa cactgctgtt cgagccaaac caaaaacaat
    20221 caaactagcc agacaaaaaa gaataatcaa aaatagagaa aacaaaagga ttgagataat
    20281 gatagtaagc aaaatcccta gatcagctcg ccatccggtt aaaataaaca tgactagaag
    20341 cggaaaaaca aaaggaatga tggtcaaagc aactaccaaa atctttgctg aaaaaacctg
    20401 ttcttgtcca aaaggcagag gcaaaaatac ttgcaggtca tttccttcaa aaaagatatt
    20461 ataaatgact gatatacttt gagttaaacc taacatacca aaaagtgcca cgtaataagt
    20521 aaagaaacct ggcaatttaa caaagttcat agcaaacata gcaaatccgt aaatcagtaa
    20581 aaaaatcagc cctgaaaaaa gattttgaga caaaagatag atagttaatc gctttccctt
    20641 ttttcctttc tctcttgctt tattcgtgag ctgtggatta acatataaaa gattaactcg
    20701 aattaattct aataattgtc gcttatacat acggtccatc tcctgtcgct tgccttccag
    20761 ccattttcaa ataaatcttt tctagcgttt catcaggtga gccattcaat aattcactaa
    20821 ctgccccctg ataaatcaat tcacctttct tcaaaatagc cagtttatcg cataactgtt
    20881 gtgcggtatc caatacatgt gtagaaaaaa tgactgtttt tccttttgcg gcatgagcct
    20941 tcatcatttc tttcaaatca aatgctgctt gaggatcaag tccttgcaaa ggttcatcca
    21001 atacccaaat atctggatct ggcagcaacg tgccgatcaa aatcgttttt tgacgcattc
    21061 catgtgaaaa acttgcgatt gtttcatttt gatgactata catatcaaaa agcgctgtca
    21121 attctgctaa acgcttttct tttttttgtg tgtctaattc ataggcagct gaaatcaagt
    21181 cccaatattc tccagccgtc aattgtaaaa aaatatctgg ggaatcaggc acataaccga
    21241 ttttttgttt gatactcaat cgattttccg tcaatttcat tccatctact aaaatcgttc
    21301 ccgatgatgg ttcaataata ctaactaaac ttttaatcgt agttgatttt ccggcaccat
    21361 tatgtcctaa aaatccaaaa atttcccctt gtcttatcga gagagacaag tcttttaatg
    21421 cttgtttttt cccgtaactt ttacttacat tgatgaattc aatcatttct gtactccttt
    21481 tgtttgttat tactttttat tgtacccttc gttattggtg gagtaaatgt caaaaagagt
    21541 gcataacaaa aaaggataga aacgactagg ctgctacttg ggttgttctc ttctacagga
    21601 aaacactctt ctaacaggta agatatgagt ctttgttttc tttaaaatga ttcattttct
    21661 atcttttctt gcataaaatt ctttagttta tcaatacttc tctttcccat ctctagttgt
    21721 tctccatttg caaatgttac tat
//
LOCUS       DAZBSA010000045        21588 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000045 DAZBSA010000000
VERSION     DAZBSA010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.045"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            56..928
                     /locus_tag="ACHGMT_001544"
     CDS             56..928
                     /locus_tag="ACHGMT_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6835289.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(1386..3584)
                     /locus_tag="ACHGMT_001545"
     CDS             complement(1386..3584)
                     /locus_tag="ACHGMT_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6835290.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            3816..4808
                     /locus_tag="ACHGMT_001546"
     CDS             3816..4808
                     /locus_tag="ACHGMT_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN6835291.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            4868..6289
                     /locus_tag="ACHGMT_001547"
     CDS             4868..6289
                     /locus_tag="ACHGMT_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFN6835292.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            6282..7781
                     /locus_tag="ACHGMT_001548"
     CDS             6282..7781
                     /locus_tag="ACHGMT_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFN6835293.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            7820..9214
                     /gene="uxaC"
                     /locus_tag="ACHGMT_001549"
     CDS             7820..9214
                     /gene="uxaC"
                     /locus_tag="ACHGMT_001549"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFN6835294.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            9373..10056
                     /locus_tag="ACHGMT_001550"
     CDS             9373..10056
                     /locus_tag="ACHGMT_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFN6835295.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            10058..10894
                     /locus_tag="ACHGMT_001551"
     CDS             10058..10894
                     /locus_tag="ACHGMT_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFN6835296.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            10905..12659
                     /locus_tag="ACHGMT_001552"
     CDS             10905..12659
                     /locus_tag="ACHGMT_001552"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6835297.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(12935..13276)
                     /locus_tag="ACHGMT_001553"
     CDS             complement(12935..13276)
                     /locus_tag="ACHGMT_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFN6835298.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(13344..15515)
                     /locus_tag="ACHGMT_001554"
     CDS             complement(13344..15515)
                     /locus_tag="ACHGMT_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFN6835299.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            15726..16583
                     /locus_tag="ACHGMT_001555"
     CDS             15726..16583
                     /locus_tag="ACHGMT_001555"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFN6835300.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(16654..17511)
                     /locus_tag="ACHGMT_001556"
     CDS             complement(16654..17511)
                     /locus_tag="ACHGMT_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFN6835301.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(17496..20198)
                     /locus_tag="ACHGMT_001557"
     CDS             complement(17496..20198)
                     /locus_tag="ACHGMT_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFN6835302.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(20211..21500)
                     /locus_tag="ACHGMT_001558"
     CDS             complement(20211..21500)
                     /locus_tag="ACHGMT_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFN6835303.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
BASE COUNT         6288 a         4370 c         3986 g         6944 t
ORIGIN      
        1 attgaacata tagaaacaga aacatttaat ttattttgag gaggagccta tctatatgaa
       61 aaagaaagtc tctataggag aaagaagttt tacacttttc aacaatacgt ttctaatttt
      121 gctagctttg atctgtatcg ttcctttttt aaatatcatc gctacatctt ttgcttctac
      181 acaagaagtc gtcgcgaaaa agtttatcct ttttcctact accttttctt tagacgcgta
      241 tcgctacatt ctttctacgc cgaccatttt tagagcactt gctgtttcga ttggtgttac
      301 aggtgtcgga acgatcgtca gtatgtgtgc aacttcactg atggcctacg gtctttccag
      361 aaagtatttg tttggtcgag gtttcgtcaa ctttcttgtt gtattttcta tgttgttcag
      421 cggggggatg attccaacgt tcttagttgt tcgttctcta gggctggtca attcttactg
      481 ggccatgatc ctgcctgtgg ctgttaatgc aatgaatatg atcattatga gaaacttttt
      541 ccaagccttg ccggatagtt tggaagaatc agcaaaaatg gatggttgca cagattttgg
      601 tgtatttttc aaaattatgc tgccattagc gttaccgtct attgctacta tctcgctttt
      661 ttatgctgtt acttactgga acacttatat gactgctatt ttgtatatca acgattcatc
      721 taaatggccg atacagattc ttttacgcca aatcgtcatt gtatcaagtg ggatgcaagc
      781 agaaagtagt gccgttgatg tgattccacc agcacaaaca gttaaaatgg ctgttatcgt
      841 tattgcaaca gtgcctatgc tgattgttta tccttttgtt cagaaatatt ttgtcaaagg
      901 agcactcgta ggttctgtca aagggtgatt atgtactgaa cgcaaaatgg tagttgtgtc
      961 agaagtggtc agcttcaaga aataagaagg catttacgaa aattgcttct taaatttttg
     1021 tgaaatttcg gcttattttt cgaagaatct actcctggaa caccgtgtat cagaggttgt
     1081 gaaagagacg ttcagccttg agcgatgata aaaactctca caaacattgc gacgagtaac
     1141 ccacaggagc aataagaagc gattttgaaa aatagcttcc catatttcac aaaattatgc
     1201 cttattgtcg atagcctgtc tcttgaacgc catttattcg taaaaaagaa ctgtgacaag
     1261 acttttgtca cagttctttt tgcgtgctta gccttgaaat ttaactatcc ataaatacaa
     1321 atactagggt tattctaact tcttttattg cttgaatcag tccacaagaa aaacagcctc
     1381 ttgattcaca aatatttttt tcgatattct cccggcgtaa tcgattcctt ctttttaaag
     1441 aaacgaataa aattttgcgg atttcgatat tgcaatcttt ctgcaatctc ttttacggag
     1501 aggttagtct ctttcaacca tttttttgct acctctaaac gatgattttg aacaaaatca
     1561 ccaaattttt ctcctgtttc ctttttaaat acattactta aatagttagg gttataatgc
     1621 aacctatcag cgatcacatc taaagacaga tcacgatcat attctgtgta gacgatatgc
     1681 ataatattct ccgacaaatt tttaaattca tgatcagtga ttgcttgaga gcattctgtg
     1741 attggcagaa ctaattgttc taaaacaagt ttttctagtt ctttgggatg ataattgttc
     1801 agtgctttta cataaatttg tttcatgtta tcaaagtttt ttgaatctac tcccaataac
     1861 tgtcctaatt gaacgagttc attgatcaag cggaccgaaa ctacttcccg actcaaagga
     1921 tttttatttt gtgcaaacaa tgcatcaatc aattcatgta ctaaattctt cacttcctga
     1981 tttccagaac gaatggcttc aaacaatcga ttttgttctt ctatcggata tctaataagt
     2041 gttttttcat gagcagcagg aacgatttcc tgataaaaaa taattgaact tggaccagta
     2101 tttacacgat aatacagggc ttcttttgcc cgatcaaccg attgcttgct ttcattcagc
     2161 gtttcgaacc ggttactgat gcctatactg atagttaaat tcaagtattc ttttacacgt
     2221 ttttgaatga gtcggcaata ctccatgact tttttgcttg cttctgcatc atctttatcc
     2281 attcgataaa tagtggcttg catttcttca ttcaaaacga tcggcagcat tcgattttcc
     2341 tttggcacaa tttccgtgat catgttgttg atcgctaaaa gtagcaaatc acgctcccca
     2401 ccgtgactat cttctaacag atcgatttga accaaagctg tataatagca gtcattttga
     2461 gaactataac caaattgctg caaccgctga tctagttctc gcctttctac tcgatttcta
     2521 aataaactaa gtacaaatag cgtttccagc tgtggctttt gggtaactaa gtgtgcactt
     2581 aacgcttctt tttctcctat gatccgactg actgattgag cgaccaaatc tagttctttc
     2641 ccactgaatc cttttgattt aagattcaga cgttcctgta tttgagaaat cggacgggca
     2701 aatctgtccg aaaataagta ggaaagaaga ccaatgagtc ctaccagtgt taaagacact
     2761 gcaactagcc cgattctcag attgcggatc attgcgccaa ttgcactaga gtcaacttct
     2821 aatctgtaaa tccaattatt ataatctgat ttcagtacta tatacgtttt atctttttct
     2881 cttatcactt tcagctgatt gtaatctaga tccacgtccg ttaatacagt tggttgctct
     2941 ttatttttta ctgtctgagt agtaaaaagg ttgccaactt cgttttcaat agaaagtatg
     3001 tcttctccac tttcaccaac aacttgttct attgtatgtt tctctatcgt agcaattccc
     3061 aatgcatatt tttctttttc aaacattggc aaagaaatca ccatttcgat tcctttgttt
     3121 ttcttttgcc aaaataaatt atttttgtta tttatgtact tctgttgata cttttcaact
     3181 tcctcttcgg tcagttgact caacgatcca ttgattactc cccatttacc agccaaactg
     3241 actaaatcat aattattatc atccataaca atcgtttcta taaaatttag ttctttcctg
     3301 atttcagaat aagtggagta atctgtatat gtcagcggat gcttgaatat gtccgagaaa
     3361 tttttggtcg aaccatacgt agaaaacgaa tattcaatcg ttctaatttt ccattcaacg
     3421 ttagttttaa cttgcctgag caaattcttt ttgtctgtta aataactcga ttcgatcgat
     3481 tcataggtat tctggtaaat gaataaacta aatcctatga caagtacggc tcctaataaa
     3541 aacatcggta aaaatatttt ataaaatact gtacttttct tcattttctt cctctcctcc
     3601 ttttaaaata aaaggctttc atttttctgt aaaattgata ctcatctaat taaatgagca
     3661 ttccccgctt tcaagaatag cataattaaa aataaaaatc caagtaattt ttaacgtgtg
     3721 caatcttttc atatcaaaga tctattgact tatcgtagtt atcgtaatat gatgaatgcg
     3781 ttaacaaaaa gtgaaaaagt aaggaaaatg atggaatggt aacgatcaaa gatatcgcta
     3841 aagcagctgg cgtttctcat acgacagttt ctcgtgcttt gaataacagc agtttaataa
     3901 aaaatgaaac aaaacaaaaa atacgacaac tagctaagga aatgaattat tcgccaaact
     3961 atagtgcaaa aggtttagtg aatcaaaaaa aatatctaat cggactattt ttctcaagtt
     4021 tgcataaagg aacttcatca aatttcctag cagattccat tgcaggcatc cattcagtat
     4081 tagacacaaa ttacagttta tctgttgaaa gtatcgatgt tgttgattta acagaaatca
     4141 acctgcaacg ctacgatgga ctgatcgtta tgagtcaaag tgatgatgat cagcctttca
     4201 tcgactatct caaagaaaaa gaatttcctt tcgttgttgt caatcgtcac atcaatgatc
     4261 cagcggttat caacgtcact gccgatgatg cggccggtgt tatgcaggca gtagattacg
     4321 gtattggatg cggacatacg aagatcgctt atattggcgg aaagaaaaat tttcgttcga
     4381 caaaagaacg ggaaaaaggg gtattgaaaa gtctaaaaaa agcgagcatc cctgtcaatc
     4441 cagcctattt tttaacagga gattacagct tagaaagcgg cctccaaaac atggaatacc
     4501 tattatcact ttctgacctg cctactctgg ttttttgcgg caacgacgac atagcgattg
     4561 gtgcattgag agctgtacat ttagctggcc tccgtgttcc ccagaacatt tctctgatcg
     4621 ggttcgatga ttctccagtc gtctcctatc tcaatccgcc gcttaccacc gtacgtaagc
     4681 ctttgaaaga aatgtgtcaa gaaggaacaa agcttttact agatttgatc gaaggcagga
     4741 cgatcgaaca atctaaaatt gagctctcca ccttgttgat ccttcgtgag tcgatcaatt
     4801 acttgtaaac tacaagtaat tttatataaa aatgttaacg tgtgcataac acagaaagga
     4861 agtttatatg aaaaaactga caaaagaatt agtgaaaaaa aacgaagcac ctgtaaaagt
     4921 tcttcagttt ggtgaaggca attttatgcg tggatttatt gattggcaga ttcagcagct
     4981 gaataaccaa caattgttta aaggtaatgt tgcaatcatt caacctttag aacaaggtct
     5041 tggaaatatg atgaaccagc aagataattt atatacggtc attttacaag gactcagcaa
     5101 tggcgaaatc gttgatactt ccgagattat caccgtagta gaacaaatga tcaatcctta
     5161 tgatgaatgg gatgcttatc tatctctagc agaaaatgac gacttggcct atatcgtctc
     5221 caatacaaca gaggcaggaa tccaattcgt ggagactgat aatttaacag atactccccc
     5281 acagagcttc ccgggcaaac tgacagcctt tttataccga cgtttcaaat taaacaaagc
     5341 tggctttacc atcattcctt gcgaactgat tgacagaaac ggcgaacgtc taaaagaaat
     5401 cattttacgc tacgcagaaa aatgggattt agaacctgct tttgttgagt ggttagaaaa
     5461 agaaaatgtt ttctgttgta gtttagttga tcgcatcgtc ccaggttatc ctcgcgaaga
     5521 tgcaggtgtt ttcaatcaac ggcataacta tgaagatcaa ttgatggtca aagcagaacc
     5581 attcatgctt tgggtgatcg aagggcctaa agaattggag aatcaactac cattcaaaca
     5641 ggctggcttg aatgtcatca tcacagatga tatgactcct tatcgtgaac gtaaggtgca
     5701 tttgcttaat ggaccgcata cagcgatggt ccctcttgct ttgttggcgg aattggaaac
     5761 tgtagaagaa gtaatgaaag atcctttatt ctccgcatat gttgatcagt tattcaatct
     5821 agaattgatt cctatgcttt ctttacctaa agatgaatta gccatttatg ctgaccaaat
     5881 caaagaacgt ttcttgaatc cttttgccca tcacaaatta gaagcgattt cattaaatag
     5941 tgtttcaaag ttcagcaccc gtctgcttcc agtgttcaag aagtatatcg aagaacaaaa
     6001 tcaggtacca ccgttaatca ctgtttcact agctgcgcta cttcttatgt atcgaggcga
     6061 tcaagtaaag ccacatgatg atgaaaagac gataagcgaa tttactgatg cttggtctga
     6121 taatggaaca gctattcctc gattattgca aaacgctgcg ctttggggag aagatttgtc
     6181 tcaaataccg aatgtgacag atacagttca agaaatagct gcaaagatcg atcaaaaagg
     6241 tgtccggtca gtattacaag aaatgaaagg aacgatggtc catgcttgaa accctgcaac
     6301 caacaatgaa attaaatcga cgagacagtg tggtcgtcgc attaacccct attccaaaac
     6361 aaacacaact gacgattgat cagcaaacga tcaccactct tgaagatatc ccgcaaggtc
     6421 ataagattgc attgggtgat ttgaaagctg gagacaacgt gatcaaatat ggctatccaa
     6481 tcggtcatgt gacgacagac gtaactgccg gtcaatggct gcacactcat aatgtaaaaa
     6541 ccaatctttc tggtgaattg gattatcgat atgaaaaaga tgtccatcca agtcattacc
     6601 tttttgagaa ccgaacattt caaggttact tgcgaaaaaa tggaaaagta gggattcgta
     6661 atgatttgtt tttagtacct accgttgggt gtgtgaatgg cattgctgaa ctgatcgtca
     6721 aacagttcaa ggaaaaacat ccagatctgg gacagtttga ccatattacg attttaaagc
     6781 atccttatgg ctgttcgcag cttggaaaag atcatcaaaa tactcgagaa attttagcgg
     6841 acgcagtaaa tcatcccaat gccggaggag ttttagtatt tggattaggt tgtgagaaca
     6901 atacagttcc tgaattcaaa aaaattttag gtgaatatga tgaagaacga gtgaaatttc
     6961 tagtggctca agatgtttac gatgagatcg aacaaggtgt ggctctatta gaagagcttt
     7021 taacagcggc ggaaaatgac cagcgcatat cagtcccgct ctctaagttg aatgtcggtt
     7081 tgaaatgcgg cggttctgat ggtctatcgg gaataactgc taatccttta cttggtgcat
     7141 tttctgacta cttgattgct caaggaggca gcacgatctt aacagaagtg ccagaaatgt
     7201 ttggcgcaga acaagtattg atggcacgag cggaaaatga agaagttttt gactcaatcg
     7261 ttgacttgat caatgacttc aaacagtatt tcttgtctta cggggaacca gtttacgaaa
     7321 acccatctcc tggtaataaa gcaggtggga tcactacatt agaagataag tctttaggct
     7381 gcacgcaaaa atcaggaagc tctcctgtcg ttgatgtgct acaatatggc gaaaaaatca
     7441 gaaaaccagg gcttagttta ttgcaagctc ccgggaatga tcttgtagct gcctctgctc
     7501 ttgcttcatc cgattgccag ttggtcttgt ttaccacagg aagagggact ccgtttggct
     7561 catatgtccc aacggtcaag gtatctacga atacaactct ttttgaacgc aaaggtcatt
     7621 ggatggattt taatgcaggc gtcttattag aacaaccaat ggaagtaatc ttagaatcgt
     7681 ttgttcaaaa aatcatcgct gtagctagtg gagaagaaac gaaaaatgaa caaaatgacg
     7741 ttcgagaaat tgccattttt aaaaacggag taacactttg attttcataa aacagtataa
     7801 aaagaaagaa ggaagattta tgtttttatc tgatgatttt ttattaaaag acgattgggc
     7861 gaaaaagctt taccattcct acgcaaaaaa catgcccatt attgattatc actgtcactt
     7921 agatccaaaa gaaatttacg aaaacaacaa tttcagcaat ctgacagaag cttggttagg
     7981 aggcgatcat tacaaatggc gcttgatgcg agcttgcggc gtgcctgaag aaaaaatcac
     8041 aggaaatgct tctgattttg aaaaattttt agcttggtgt caaaccgtac caaaaattat
     8101 cggaaatccg ctttacagtt ggtcacatct tgaattgaag cgtttcttcg gtattgactt
     8161 gttgataaat gaagaaaatg cagagaaaat ttggacacaa gcaaatgaaa ttttggcagc
     8221 tcctgatttt cgccgtcggg aaatcgcaaa aaaatcgaat gtagaagtga tttgtacaac
     8281 agatgatccc gcagacgact tgcactatca tcagttgctt gctcatgaag aaccagattt
     8341 gaaggttcta ccttcattcc gtccagataa agcactcaat attgaaaaag aaggatttaa
     8401 tgcatgggtc acactactgg aagaagcagc tgaccaaacg atcaccacct atcaagaact
     8461 gatagaagtc ttagggcagc ggatagaata cttccatcaa catggatgtc gtgtttccga
     8521 ccacgcttta gacatcttac gctataaaga agcggatgaa acagtattag aagagatttt
     8581 tcaaaaagca cgagctaaca aactattatc ttcagatgaa atcgatgctt atcgtacaga
     8641 aacactgata catttgaccc acttctatca ttctcataat tggacgatgc agctgcacat
     8701 ccatgcttat cgtaactgta atacacaaat gttggaaaaa ttagggcccg ataccggcta
     8761 tgatggaatg aatgatcttt ctttgactat tcctctgcaa aaattattga accgtgcaga
     8821 agaaacggat cagttgccaa agacgatcct ttattcattg aatccgaatg actatcctgc
     8881 gattttagct ttgatgggct gtttccaaaa agaacagaat ggaaaactgc agttaggttc
     8941 tggatggtgg tacaacgata ctcgagctgg tatgcgcgat caattgacac aatttgctga
     9001 tggtagtgct ttggggaatt ttgtcggcat gctgacagat tcaagaagtt tcctgtccta
     9061 cactagacat gaatatttcc gtcgtgttct ttgtgaattt ttaggcgaaa tggtcatgcg
     9121 aggagaagca cctgaagatg aacaactttt aggaagtcta gttcaagcga ttagttatac
     9181 aaatgctaaa aattactttg gcttttttgc ctaattgatt aatagtaaca gtaacgaatc
     9241 aaaaacagga tagtgaaata acggaatccg tttatccacg ttttagagaa gaagacggac
     9301 caaatttgct ccgtcttctt ctcttgctgt tctgcttcat tgcctataaa cctttatgaa
     9361 agaagagata acatgcctac tatccatatt gccggtgatt ctactgcggc agaaaaagat
     9421 gaaaacaagc gtcccgagac aggttgggga gaaaaaatct ctgcttattt ttcacccgag
     9481 atccagatca ataaccaagc aaagaatgga cgcagcacca aatcatttat ccaggaagga
     9541 aggttagccc gtctagcttc acagtttcag ccgaatgatt tcttatttgt acaatttgga
     9601 cataacgacc agaaaatcca agaagaaaga ggtacgcttc ccttcggtag ctatttaaaa
     9661 aatctgcaga tattttttga atgcgcaaaa aaagcgaacg tccaaatggt cttactgaca
     9721 tctgtgacgc gacgagatta cttagaaaac ggaacattga atccagatat acttggagac
     9781 tatccaaaag cgatgcgtgc atttgctgag aaacaccata ttcctctttt agatgtgttc
     9841 tcaagatcac aagaattgtt ccagaccttc tcaaaagaag agacaaaaaa attctacctg
     9901 catcttatac ctgatacttg taaaaattac ccagaaggac taaaagacaa tacccacttt
     9961 agtccagaag gcgctgataa agttgcccga ataattgttg aattgataaa agaatcccgt
    10021 ctgcctcttg ctaattattt acaaaaagga gtttgagatg tttccattaa atttaggcat
    10081 ccgtgcccac gatttagatg ttcgaaatcg tgaagaaatc gttcaaaaag tatcacagct
    10141 tcacttgtcc catatccaat ttgcaccaca aaaagcattt cctgaacaac ttaaattaac
    10201 tgacatgaca cttgggagtg ctgcctattt tggtgaatac ttccaaaaaa acaatatcga
    10261 actttccatt ttaggatgct atatcaacct atccagcaaa aatcaagaag ttcgtgcaca
    10321 agcattggca actttcaaac agcaaattgc tctgtgctct gcctatcaag ccaagatgat
    10381 tgctacggaa accggtagtg tctctgtcgg ttacacaaaa gaaaatttta ctgaagaagc
    10441 ttatcagatc gccagaaatt ctatcattga attagtcgct catgcagaaa attttggcgt
    10501 aactgttgct gttgaagcag ggatcaatca tccaatctat aactatcagt tagctaaacg
    10561 tttaatcgat gaagtagctt caccaaattt aaaaatcatt ttagattgtg cgaatttgat
    10621 gcataaagaa aatcatgctg atcaggaaaa agtgatccga ggtgccttgg acaatttaca
    10681 tggttatatt acagcgcttc atctcaaaga ctatatcatg gaaaatggaa agatccgcat
    10741 ggtacctgtt ggcaagggct gtttagattt ccgcccggta ctgcattata tcaaatatga
    10801 gcgtcctcta atgtacgcca ctcttgaagc tactcctgag atttatgttc ctgaagcaat
    10861 agaacacttg cagtttattt accaagattg ttaaggagaa gattatggag gaacgaatac
    10921 agaaaagtta tcaaaaatat catgcaaatg cttttcagac attgctgcat ctttatcagg
    10981 gaaattattg gcgttttgcc atgtcaggac tcttttttat tatcaaacat cttccatctt
    11041 ggttaatgcc gatcgctatt gccaatgtaa tcaatgcagc ttcatcgaaa gattcttctc
    11101 agttgcaggc catttatttc aacgcttttt tgatggcttg tctgattggt caaaatatat
    11161 tgacgaatta ttttcatgta aaataccata gcacttcgat ccgtcaagta gaagctggac
    11221 taagagcgac ccttatcaag aaaatccaag aactatctat tccttatcaa aaagaattgc
    11281 aatctggacg aatccagtca aagatcatcc gtgatgtaga agctatccaa acgttgtcct
    11341 ctcaagtatt tgtcagcgga ttaaatatcg ctgtcaattt attcgtagct ttaggtatca
    11401 cgctttataa aagtcctgtt gttttcatgt ttttcttatt gactgttcct ctcgccgcat
    11461 tcgttactct ctttttccgt aaaaggatca gacagtccaa ctcagacttc aggcaagaaa
    11521 tggaagagac ttctgcaaaa gtgatcgaag tgatcgaatt agcaccgatt gctcgagctc
    11581 atgggttaga aaaacacgaa acaactcgct taaataagca ttttactcaa atctatgaaa
    11641 aaggattcca cttagatatc ctgcaatctg ttttcggatc agtcagctgg gctagttttc
    11701 agatatttca gctgatttgt ttgattttca ctgggatatt agctttacaa gggcgcatcc
    11761 aacccggcga tgtcgtgatg tatcagacgt atttcacgac gatcgtcaat tccgtttctg
    11821 gaatgatcac gctgattcct acgatttcaa aaggcatgga atctatcact tctattggcg
    11881 aactgttgat tgctggcgat gtagaaactt atcacggtaa gaaaaaaatc caacatttgg
    11941 atggaaattt tcgttttgag gatgtttctt ttctttatcc ggactcactc aaaccgatta
    12001 tcgatcattt ttctctagag gtgaaagctg gagaaacaat tgcttttgtt gggccatctg
    12061 gctctggaaa atctacactc cttaacttat tgattggatt tatccagccg acttcaggaa
    12121 aaatttttct agatgatcag cctttggatg aactggatat gcgaagtgtg cgaaattatt
    12181 tagccgttgt tcctcaaacc actttgttgt tctccgcttc gatcaaagag aacatcactt
    12241 atggcttgaa aaacgtatca aaagaacgtt tagatgaagt catcgaagct gcgcaacttt
    12301 cttcactgat tgaccagctt cctgatggtc tggatacctt agtaggcgaa catggcaaca
    12361 agctgtctgg tggccaaaag caacgtatct ccattgcacg agcgttgata cgtcagccaa
    12421 aaatcatttt gctagatgaa gcaacctcag ctttagataa ccagtcagag aaaaaaatcc
    12481 agcaagccct gaattacctg acagaaacac ccactacttt tattgtggct catcggcttt
    12541 caacgatcaa agaggcagat aaaatcgttg tgatcgatga aggaaaaatc gttgaaatag
    12601 gtacttacga aaaactgtta gctcaaaaag gctatttcta ccgcttgaaa aatgaatgac
    12661 aaaaaatagg agctatgaca agacttttgt cgtagctcct atttctgaat aaacggtgtt
    12721 ctcctgccaa ctcctcggtc acaagtcaca atagtcatca aacatgagaa gctgtttttt
    12781 gaaattgttc cttgtaactc agagttaaac ggtaggctca cgacctcttg gtaaatggtg
    12841 ttcaagagac agcctatcgg caataagaag caattttgtc atcgctcaag gccgaacgtc
    12901 tctttcacac cctctgttgt ttcataaaat agttttattc gctgtaaagg tcgcgaactg
    12961 gtgtcatgat gatacggttc aagtcattga tgattgtact gaagccttgc tctttttgca
    13021 tcaagtcatt gattacttct tctgattgga ttttcacagc catttcttgt gctttcattg
    13081 catcttcgtc gctgaactct tgtccactca tttgtttttc ttgtaattcg atttgcatcg
    13141 cttggaagtc tttgaacagt ttgtgtgctt cttcgtttgc ttgtactttc tcgtacgctt
    13201 ctttcaacgc tttgaattct ggtaattcac ggatctcacg ttcgatttga ttcgcgctat
    13261 cgtagatgtt tgacatgtta tgccctcttt tcttttagta gttactctct attgtaacat
    13321 ggggttattc gttttcaaag gggctactct ccttgatatt tccgccagag gtctttagcc
    13381 gcttcaccaa ttccctgaac gccttctttc accatatcgc cgaagttttt cagcccttct
    13441 tttgcttgtt caccatattg attcaagtta tcttgccaat ttccagactc tccattagaa
    13501 tttcctgtgt ttccaacttg atctgcagct accacttgtc ctcctgtggc ataggcatct
    13561 gctacactaa atcctgtttt tgaagtatac ggaaggatgc cttctgccgc tgatttgaag
    13621 actttcccga caacatttcc acttgtccct tccaaatagt gcagcttgct ggattcttcg
    13681 taacccatcc atgtcgaaat cactacgtct ggcgtgtaac cgatcatcca ttggtcattt
    13741 gcttttgtgg catcgaaatt agtttctgtg gttcctgttt tccctgctat cgtataacct
    13801 gcaggattcg cagcagccgc tgttccattt gaaaatgtac caagaagcat gctggtcatt
    13861 tcatcggctg tttcctttga tatgacctgt tttggttttt tgtttgtgtt atcgacgatc
    13921 accgctccag tcgaatcaac gattttcgtg ataagatgcg gcgtatacat ctgaccatca
    13981 ttggcaaatg cactgtaagc acccgccatg atcaatggag atactccttt ttccagtcca
    14041 ccaagggcta agccataata cttatcagat tttgctaaag gcaggccgaa ttcttctgct
    14101 ttatcatatc ctttttgtaa accgatctca tgcaaaagcc atacagtagg caaattcaaa
    14161 ctttgtgcaa cagcttgata catcggtact tctccttggt atgtcccgtc aaaattatgc
    14221 gcatcgtaat aactttgcgg ttcgtctttc aagatactgg aaggcttgta tccagcttcc
    14281 agtgctggtg tatatacgct taatggtttg atcgttgatc ctggtgaacg acgcatctgt
    14341 gtagcaaagc tgaatcctct aaagacatga tctcctcttc gtcctactag tgcttgtaca
    14401 ccacctgtat tcggatctaa agctactgaa gcagattgga ccatggcacc atctgaagca
    14461 tttgctggga ataatgcact gttttgataa gtagcttcta attgcttttg ataatcctga
    14521 tctaaagctg tatagatttt atagccttta ttcatgacat cttcttcatc aagaccatac
    14581 tcgttaactg cttcatcgat cactgcatca aaataataag gataacgata accagaatct
    14641 gaattatcgt atgtgtcatt aagatacgcg ctgatatctg tctggctttc tgcttgttct
    14701 tgttcttttg tgatttttcc gttttcttcc attacactta gtactgtgtt tttccgatca
    14761 ttcgcatttt ttgggtgatc gatcggatta taaataccag gacctttcag cattcctgcc
    14821 aaagtagcag cctcagacag cgtaacattc gttgcatcta ctccaaaata ctttctggaa
    14881 gcgtcttgga caccccatac cccgttgcca aaataggcat tgttaagata catggtcaag
    14941 atttcttctt tgctgtactt cttttcgatc tcgattgcca aaaataattc cttcgctttt
    15001 cgatcaaacg tttggtctaa tgtcagatac gcatttttgg caagctgctg agtgatcgta
    15061 cttcctccgc cccctgatgt tccgccagac aagaccattc ggactgctgc acgggcaatc
    15121 cctttgatat caaacccgtg atgctcgtaa aaattcctat cttctgtcga gattactgca
    15181 tcttggatat agggagaaat cgcgtttaat tctacatatg ttcctttttg ggcgtacagt
    15241 gtgccagctt cttcgttttt cttatcataa atgactgtcg attggcttaa tcctgatttt
    15301 aacgtttcga catttgcttg ttttgctaaa ataaagaggt agatacttgt cactaagaca
    15361 cagatcaaac caatcagcaa aatcaatttg ttcacatgat attttttcca gatgcgcttt
    15421 cgccatcgat ggaattgtcc gagatatggt ctgatccaac gccaaaaact gatcaaagca
    15481 gcttttatct tttctaaaat gctttgaaaa tccatttgct tcttctcctg tctatttgat
    15541 tcaatcgtct tattttacca tagtgaaaga gtgttttctt cctgcttaag ttgtagttca
    15601 atatgttttt ttacgttagt gcaagtagag taacataaga atgaacagaa aaatgtaagt
    15661 tttttcttaa atatgtttta aaaatggtac aattagtctg cgattgcact gaaaggagta
    15721 cacttgtgaa aatcacgatc actttaccta tgaaccatcc tgaaacaaca attcgcgact
    15781 tgcttgaaaa ggaatggtta gttcctagaa aggttcgaca ctttttgcgt atacgtaaaa
    15841 acgtttggat caatcaacaa cctgctttgt tccatcatga agtaaagggc aatgaccaga
    15901 tcacattgtt attcgaagaa acagattata attatcaaga agttcaatta ggagatgcga
    15961 agcacatccg tgttttatat gaagatgagc atttgattat agtggataag cctgcaggaa
    16021 tgaagactca tccaaatgaa ccgacagaaa acagtacctt actcaatcat ttagctgctt
    16081 atctcgacac taaacagcaa cgaccttacg ttgtccaccg cttggacaaa gaaacaagcg
    16141 gtgccgtttt atttgcaaaa aatccatttg tcttgccgat acttggaaga atgttggaac
    16201 aaaaaatgat ctatcggaga tatcaagcca tcgtatgggg agaaattgcc aaagatatga
    16261 ccatcaccaa taaaattgga cgagatcgcc atgatcggag aaaaagggtc gtagatgata
    16321 aaaatggtca gagcgccttg acacatgtca ctgtcaacaa agtactttct gaacaaacac
    16381 aagtctattg tgttttggaa accggacgta cacaccagat cagagttcat ttagctcata
    16441 agggacatcc gatcgttgga gaccctctct accaaaatag aacagctgaa cgactgatgc
    16501 tccatgcatt agaactgcac atgatccatc catttactca agaaaaaata gtcggtaaag
    16561 ctttacctgg attatgggaa tagaaataaa aaagaacaga gtcttatcag cagataatga
    16621 taagactctg ttctttttgt gtaatttgtc tgcttacttt tgatgttcgt gttctgctaa
    16681 cagcaaacta ccgataatcc cactttcatt cggcagtccc caacgaacga tatattcact
    16741 tacttgaggt gtttccatat aaccacccat caattcaaca aattgctgac ggattttttg
    16801 aaggagatga tcctgattca ttaccccacc gcctaaaata atcttttcag gtgctaacgt
    16861 caacgtatag ttgatcagtg cttgagcaat ataataagcc tgaatagccc aaacaggatg
    16921 atcttgcggc agatcttgcc ctttgatccc tgtgcgtgcc tcaatagacg gtcctgctgc
    16981 taatccttct aaacaatctt tatgataagg acaagtacct tcataatggt catctggatg
    17041 gcgtttcacc caaatatgtc ccatttcagg atgtgcgatc cctgaaaaaa tttcttcatt
    17101 gatcacgaca cctgcaccga ttcctgttcc cacggtaagg taaatacaac tattttttcc
    17161 ttgtgctgct ccttgcttca attccccata tgcagcagca ttgacatccg ttgtccaagc
    17221 agcaggaata ttgtagcgtt ccttgattgt gccaagaaaa tcgaattgtg cccacccttt
    17281 tttgggagtt gccaaaacgt atccgtaatt atcttttgtt ttgtcgatac caatcgggcc
    17341 gaatgagcca atcccgattc ccttcaattc ataacgatca aaaaattcaa atacctgatc
    17401 caatgtttct tctggtgttg tggtagggat acttaatttt tctaaaattt ctccaccatc
    17461 tgaaaccgca cacacaaatt ttgttccgcc tgcttctatt ccgccataca ttcgttttcc
    17521 tcctcgaatc cgattgtccg aatctcataa ggttggatta ttgggacaac cgcttgcttc
    17581 gttggttctt caagcagatt caatatgaca gtttttccat tgtctttagt tatttccaat
    17641 ttttctaaat gactagacat attgaaacct cttactacta ctttatcact aaacttgctg
    17701 cgtttcaaag ctgtaattgc aaacgtttca ctttttatcg ttaaatattg ttgttttgaa
    17761 atcaatgttc cttcatgatg tttgatttgt tgagtggaaa aaggaatctg tgctgcgtat
    17821 gcatgcaaat aagtagagaa tttttcttct ggtccatgta gttcaatgct gtaattgaac
    17881 cgatgctcac ctagacactg tgcttctggt gttggaaaat atccccaatc gcctagttct
    17941 cccacactgc gaagtagcgt cactgcgatt tgtccgtctt ctgttacttc atattcattc
    18001 aaaccaaagt ttccgactgt tacaccgtac tcttcatcat gaatattgac aaatgcttgc
    18061 tgatgttgcg gattcgtggg gttttcccaa cttttagaca catggtttgg gcgcttcacc
    18121 acttcaaaaa tactatctgc ttcgtgatgt tccacatgta actttgtagg aaacaagaca
    18181 cgtaagcgat gatctttcat ctgattgtcg agagtcgttt caaagtcgat ttttttacta
    18241 tcttttctga ttgtcatgat tgtttttatt tgaaggattc gtttttcttt ggatcgttct
    18301 gcttttcgat aacgaaattc cacaaccatc tgctgttctt tttctaaacg ctcgtctgca
    18361 gaaacaggga tttccatttc ctgtagaata cttatttttg ctatttccgg tgtatctact
    18421 aatactttta cttcagagtt ttctttgtta ctggatagga ttggctgatc acagaaaggt
    18481 tgtttaaaga tatactcatt tcctatatcg ccgatatctt caaaaatatg cagattattc
    18541 aactttcgtt tgtttttgcg atcttccatt gtaatggtac catttttttc aatcgtcaaa
    18601 tgtagagggc cgttttcgat gattcgtcca ctctgatgaa tcattgtttc cttttcttct
    18661 gctttcgctt ccccttctac aagcgcgaat gtttcccaag aaaatgctgg catgttttct
    18721 aatggtaaag tcactttgat cattcttgcc atatatggca cacggaaacg gtctttcggc
    18781 aaatcataac caaattggac agtttcttca gataaaatag ctggtatagt ggccccactt
    18841 tttgtttcta catgatactt acgtttaggc aaattttcta actcttgata tagttgttca
    18901 ggtattcctt cagcgaatct ttttctttct aattcgatgg ttatttctgc ttcaccagtt
    18961 ttatccattc ctgcagtatt tacaatgaca aatggaaagc tgtcttttgg gaatccggtc
    19021 gtatcgattg ctgcggtcag ctgttctaag gaatcttgtg caagatactt tccaacttca
    19081 tctgcttttt caaaacgagg tatcatttcc cgatggacag aatcgactga acaaccgcaa
    19141 atactgtcgt gaggatgatt ttgcattaat gtcttccaag cataatctaa ttgatcatgg
    19201 ggatagtttc cactaacttc ataagccatc gtcgcaagtg gttctgtaat attttctagc
    19261 tgtcgctgga ccttggtatt ccattgtttt aagtaaaccc gagcagatgc tgtattggct
    19321 aaagtatacc agccatccgt ttcttgactt gtcaattctc cttctactga gcctaagtct
    19381 tctggcacat ctttttgaac tgcttccaga tagtcagtaa aattagaatg aataaactcg
    19441 taatctggaa acagttcatt tgcgagatgg attgcttttg atatatcttt ttgtaccggt
    19501 tgatgatcaa caccattcat catcaataag tggttcgtag aggcatattg ttctgcatcc
    19561 gctaattttt gcttccaaaa ggcaagagct gcttcttttt ctactggaat ctcattcccg
    19621 ttgctatacc aattggcaaa tagcagacca aagattgctg tttgatcagg gcctttccac
    19681 cacatctctg agtattggga agaatagttt tctgcttcca aaacttgatt atcaaagcca
    19741 attggtttta caccacggcc aaacgctgct gctgaaatac cagcttgttt cattaattga
    19801 ggcgtctgtc ccatatttcc aaatgtatcg ggaaaatagc ctaacatgac aggggttccc
    19861 catttacggc tttcttccat gccaatcagc atatttctca cgttcgattc cgagctgata
    19921 agaaaatcat cttgcaagat ataaaatggt ccaattctca attttccttc attgattgct
    19981 tgttggattg cctgtctttt ttctggcctt acttgaagat agtcatctaa aataatcgtt
    20041 tgcccatcca aatgaaaact gttgaaggac ggatcaactt caaatagctc tagtagatcg
    20101 tctatcaatt caatcagtcg catgtgatgc tgttcgtaag ccatgtacca ttcacgatcc
    20161 cagtggctat gagaaatgat atatactttc tttttcattt acgatacctc ctacttttct
    20221 actctaatat caaagtaatc catgactaat tcgcaaaaca tcatatttgc ccaagaaaac
    20281 cattctctcg tataattttc aggattgttc acatcgaatc cttcgtgcat aaggtgtgtt
    20341 cctgcgtcag ttgctacaag taaattcaat atccgctctt tttcagattt gtctgacgtt
    20401 gtcattcctt ccatagctaa tgcaatcggc caaacgtatt tttctggcgt atgggaggat
    20461 ccaattcctt ttgcgtactt tccttcatag aaatatggat tttctttact caacaatgtt
    20521 cttcttgttt gcagataatc ttcctcttgt tcagaacaat atcccaagta aggtgcggcg
    20581 atcagattgg gcacattact gtcatccatg actgtggcgt ttcccattcc atctacttca
    20641 tatgcataaa tcgtttgccc tttttgatta accgttcgtc cgtgattctg aatcccctct
    20701 tggatctctt tcttcaattt ctgtgtttgc tgtacaattt ctttatcatc gagtatcgtt
    20761 tgaaaaattt cttccacata acctaacacg accaccgcaa acatattgga tggtaccaga
    20821 tatccataac ggcaagcatc atcgctagga cggaaaccag accatgtcat tcctgtaaaa
    20881 gctgtttttg cacctcgccc gtcattgatc agcgtatctt cttttcttgt cgtgtctcgt
    20941 acaaatgtat aaggagagtg ttcatgatct tgctctgttt tgaacacgtc caagattttt
    21001 ttgatacctt caataaaaga ttgattgaat tgatcagttt ttcctgtatt tttgtaaaga
    21061 agataggcca actgtattgg gtagcataaa gaatcaattt catattttct ttcccagatc
    21121 caatcgttca tttttgtatg atctgtttgg tggccagcac cgttagcttc ttcgttgaag
    21181 gcattcgcat atggatcgat attgatataa aaaaattgct ttttgactaa cccgctgatc
    21241 atgtcagcta attcctcatc ttcttttgca atcactaaat aaggacgaac ttgtgcagta
    21301 gagtctctta accacatcgc tggaatgtct cccgtcaata aaaaggtggt tccgtcattt
    21361 tgctttttga ctgttgtcaa cagagtattg gcaaatgctg ctgtaaaatt ctttgcccag
    21421 tccgcatgga tatccccaca tttttgtgtg atatcttcca taaatttctg caccgatttt
    21481 ggtatatctt tataaaccac taataaaacc ttctttctat ttgatatatc tttaaagatt
    21541 atacttcata cagccaatta tgtctacgct ttcaaaaaat atcttttt
//
LOCUS       DAZBSA010000046        21574 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000046 DAZBSA010000000
VERSION     DAZBSA010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.046"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(291..1787)
                     /gene="lysS"
                     /locus_tag="ACHGMT_001559"
     CDS             complement(291..1787)
                     /gene="lysS"
                     /locus_tag="ACHGMT_001559"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFN6835304.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1898..2902)
                     /gene="dusB"
                     /locus_tag="ACHGMT_001560"
     CDS             complement(1898..2902)
                     /gene="dusB"
                     /locus_tag="ACHGMT_001560"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFN6835305.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2903..3790)
                     /gene="hslO"
                     /locus_tag="ACHGMT_001561"
     CDS             complement(2903..3790)
                     /gene="hslO"
                     /locus_tag="ACHGMT_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFN6835306.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4007..6118)
                     /gene="ftsH"
                     /locus_tag="ACHGMT_001562"
     CDS             complement(4007..6118)
                     /gene="ftsH"
                     /locus_tag="ACHGMT_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFN6835307.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6208..6753)
                     /gene="hpt"
                     /locus_tag="ACHGMT_001563"
     CDS             complement(6208..6753)
                     /gene="hpt"
                     /locus_tag="ACHGMT_001563"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFN6835308.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6753..8198)
                     /gene="tilS"
                     /locus_tag="ACHGMT_001564"
     CDS             complement(6753..8198)
                     /gene="tilS"
                     /locus_tag="ACHGMT_001564"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFN6835309.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8318..8788)
                     /locus_tag="ACHGMT_001565"
     CDS             complement(8318..8788)
                     /locus_tag="ACHGMT_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFN6835310.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8834..9259)
                     /locus_tag="ACHGMT_001566"
     CDS             complement(8834..9259)
                     /locus_tag="ACHGMT_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFN6835311.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9326..9595)
                     /locus_tag="ACHGMT_001567"
     CDS             complement(9326..9595)
                     /locus_tag="ACHGMT_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFN6835312.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9606..11201)
                     /locus_tag="ACHGMT_001568"
     CDS             complement(9606..11201)
                     /locus_tag="ACHGMT_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFN6835313.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11216..14737)
                     /gene="mfd"
                     /locus_tag="ACHGMT_001569"
     CDS             complement(11216..14737)
                     /gene="mfd"
                     /locus_tag="ACHGMT_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFN6835314.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14754..15314)
                     /gene="pth"
                     /locus_tag="ACHGMT_001570"
     CDS             complement(14754..15314)
                     /gene="pth"
                     /locus_tag="ACHGMT_001570"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFN6835315.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15667..16641
                     /locus_tag="ACHGMT_001571"
     CDS             15667..16641
                     /locus_tag="ACHGMT_001571"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFN6835316.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16811..18211
                     /locus_tag="ACHGMT_001572"
     CDS             16811..18211
                     /locus_tag="ACHGMT_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFN6835317.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18289..19011)
                     /locus_tag="ACHGMT_001573"
     CDS             complement(18289..19011)
                     /locus_tag="ACHGMT_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN6835318.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19090..19596)
                     /locus_tag="ACHGMT_001574"
     CDS             complement(19090..19596)
                     /locus_tag="ACHGMT_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFN6835319.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19719..21518
                     /locus_tag="ACHGMT_001575"
     CDS             19719..21518
                     /locus_tag="ACHGMT_001575"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFN6835320.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
BASE COUNT         5996 a         4711 c         3873 g         6994 t
ORIGIN      
        1 ttagtaacat gtcaataact tttttgttat gtttttgaat aaatattttc ttctgtcttt
       61 tggacaagta ataatatacc aactaaattt agcttggtca acgaattttt tgtatttttt
      121 caagtttttt tcaataaacg attattcata acttttcttt ctttatcgtt cgttttcgtc
      181 ttctttaatg tctagaatag tcggcattag cttttgaact ttcgttatta tttattcctt
      241 tctacacagc aaaagctgcc taaggaattc cttagacagc ttttattcga ttatctcatt
      301 gttgggaata gcaaaacatc ccgaattgat tgtgcatcag ttagcaacat tactaatctg
      361 tcgatcccga tgcctagacc accagttggc ggcataccgt attctaaggc ttccaagaag
      421 tcttcgtcta caccgtgagc ttcgtcattt cctagttcac gttctttttc ttgcgcttcg
      481 aatctttctc tttgatcaat tggatcgttc agttctgtaa aggcattagc aaattcgcgc
      541 cccactatga atagttcgaa tcgatctgtg aatcgaggat cttctggatt tttcttcgct
      601 aaaggcgaaa cttcgactgg atgtccgtat acaaacgtcg gttgtgtcaa tgtttcttca
      661 acaaatgttt caaagaattc attgataaca tgtccgacac ccatagcttc ggttacttct
      721 acattgtgtt tttcggctaa agctaatgct tcttccaatg tcatttcttg ccagaagtct
      781 acaccagttt gttctttgat tgcatctacc atatggatgc gtttaaagtc actttctaag
      841 tcgacagctt gtccatcata tgtgattttt gctgttccta gtactttttc agctgcgtta
      901 cggataatac cttctgtcag attcattaca tcattgaaat cagtatatgc tgtgtatgct
      961 tctaacatcg taaattctgg attatgtgta gtatcaattc cttcattacg gaaaacgcgg
     1021 ccgatttcat aaactttttc cattccgcca acaattagtc gttttaaatg aagttctagc
     1081 gcaatacgca aatataaatc aatgtccagt gcattatgat gcgtgataaa tgggcgtgcg
     1141 gcagctccac ctgcctcatt gtgcaatact ggtgtttcta cttcaatata gccattgcca
     1201 tctaaatagc gacggatttc gctgataatt tggcttcgtt tcataaatcg gtcaaagctt
     1261 tcgcgattac tgattaaatc caagtagcgt tgtctgtagc gttgttcaat attcgttaag
     1321 ccatggtatt tatctggcag tggacgaagt gcttttgaca aaatcgtgat ttctgaagct
     1381 ttgatagaaa cttcgcctgt gtctgttttc atgatttgtc cagttacacc aaagaaatct
     1441 ccaaggtctg catgtttgaa tacttcatag gcctcatcac ctacttggtc ttttcttaca
     1501 taaatttgaa tttggccttc acgatcttgt agatgcgcaa atccagcttt cccttttcct
     1561 cggcgagtca tcatacgacc agctacacta gcggtcaatc ccatttctgc tagttcttct
     1621 tttgttcttg gatcaaatag ttcatgcaat tcttctgagt tgtgcgtgcg ttcaaatcgt
     1681 tttccaaatg gatcgattcc atcttcacgc aattgttcca ttttttgacg acgtacaagc
     1741 atttgatcat ttaaatcttc tacttgatgt tgttgttcct gtgtcacaaa agttcctcca
     1801 ttctcatgtt aatctctttc ctataatgcc aatgaaacac aaaaaaagca agccgaactt
     1861 ggaatttcgg cttgaaaatt cacgaaaaac ttagaaatca caacgtttgt ttcaatagta
     1921 ctttttccgc tttttctaca aattgatcca gcaggtcatt gatttcttgc tgtttttctg
     1981 tctgattgat tgctactttc actttcgctg ctcgaggaat cccttttaaa taataagccg
     2041 catgtgttct gaattctcta caagctactt tttctccttt gagatctgct aagcgttgta
     2101 aatgaagttt agctgtagca attttttcgg ctggtgttgg ttcaggaatc agttcccctg
     2161 tttcgagata atgctgtgtg cggtgaatca tccatggatt gcccagcgct gcacgaccaa
     2221 tcatcacacc gtctgcaccg acatagtcaa gcatccattt tgcatcttct ggcgttttga
     2281 catccccgtt ccccataaat ggaatcgtca aatgtttttt cacttctttc aatacttccc
     2341 agtccgcttt tccttcatac atctgaaccc gtgtacgtcc atgcatagcg atggcacttg
     2401 ctccagctga ttgtgctgct aatgcatttt ctacagcaaa gacgtgctct tcatcccaac
     2461 caatacgcat cttgattgtt actgggatat ttactgctga agaaacagct tggaccattt
     2521 cgtatacttt ctcaggatct aaaagccatt ttgctcctgc ttctgctttg attactttat
     2581 ttactggaca gcccatattg atatctatta tatcggcagc tgtgttttct tcgacaaatt
     2641 gagcagcttc aactagacta tctttatttc cgccaaatat ctgtaaactt aatggatgtt
     2701 ctgtttcttc aatgtggagc atctctaaag tttttttgtt tctgaaatga ataccttggt
     2761 cactgatcat ttcacatacg actaagcccg caccaaattc tttgacagtg actcgaaatg
     2821 ctgaatttgt tattccagcc atcggtgcaa caacgacacg attagggatt tccacattgc
     2881 caattttcca tgtgtgttcc acttaaatcc ctcctaaggc ttctttcttc aatccatcta
     2941 attcttcttc gctatacttg tatttgttat tacagaaaga acaaaccgct tctgcaccgt
     3001 gatcttcgtc tatcattgct tggatttctt ctgcacctaa cgcgataatt gcttctgcaa
     3061 atttttcttt tgaacagtca catttgaatt gaacaggcat tttttccaaa atgttcaatt
     3121 cttctgtacc aagcaatcct ttcaatattt cttctggtgt ctgtccttca tctaagaggg
     3181 tagagattct tggtgtcgtt tttaattgtt cttcgatctt gctgattgtt tcttcatcgg
     3241 cacctggcat gatttggatc atgaatcctc ccgccgtctt gatgctgtcg tcagtattga
     3301 ccaatacact cagtccaaca gctgaaggga tttgttctga gatagcaaga tagtaagtaa
     3361 aatcttcagc gatttctcca gaaacaatcg gcgtttgccc agaaaaaggt tcttttaatc
     3421 ctaagtcttt tgtgacagta aacattcctt gtgttccaac tgctccccgc acatcgattt
     3481 taccttcact atttagagga agactgagat gcggattttt gatgtaacct ttcacatctc
     3541 cctttccatt tgcatctact acaattcctg ttgctggacc atcgccttgg attcgaacag
     3601 ttagtttgtc ttctcctttg agcgtcgcac caagtagcag actaccgacc aatgtccgtc
     3661 ccaaagcagc tgaggaggtg tgccaagtat catgacgtct ttgtgcctcc gctacggtat
     3721 ttgttgcttg aacggcgtat gctcggacga atccgtcgtg agcaagtgcc ttaaccaaat
     3781 aatctttcat ttgttatgta ctcctttttc cgtttttaaa ctcatagtga atcatactag
     3841 caactactgc ttttttcaac tttggaatgc ttcttttaaa aataattcag aatcatctag
     3901 aaaacaggac atcaccctat ttacaactta aataaagaaa tctcccgaaa accggttttt
     3961 gttccggttt tcgggagtca tatcagatca attgacctat atttttttat ttgtattgat
     4021 cgtctgaatg attatcttct gattgttgag aatctgaatc atgttctgct ttattttcct
     4081 cattcagttc atgttttgct tcttcaaaat cacgtttttc ttccgcttgt ttttcagcgt
     4141 ctttttcttc caaagcacgt tttgcttctt caaaagtttt cgcttctttt tcgcttggat
     4201 aatcatttga ctcagtacct tcaggcatca caccgtgttc aaacaatgac ttgattgctt
     4261 tggcatccaa tgtttcgaat tccaaaagtt tttcagcaat cagtttatgc tgtgcacgat
     4321 gagcttcgat gatttctcga gctttatcat gagcttccat caagattttt cgtacttctt
     4381 gatcaatctc gaaagcgact tgttccgagt aagcttttgt ttgaccatag tcgcggccaa
     4441 caaacacttg atggtttcct tcatattgaa caggacctaa acgatcactc attccatatt
     4501 ctgttaccat gcttcgtgct agagctgtcg cttgctcaaa gtcattggaa gcaccagttg
     4561 attggacatt gaagatgatt tcttcagctg tacgtccacc aagtaagcct acgatttgtt
     4621 caaacatatc ttctttcgtc atcaagaact gatcttcttt tggtaaagcg atcatatatc
     4681 cgccggcgcg tccgcgaggg atgatcgtta ctttgtggac gacccgagcg cgactcaata
     4741 ctaatccgac aatcgtatga ccagcttcgt gataagcaac catttcgcgt tcttttttgc
     4801 tgattacgcg atctttttta gctggacctg caatcacgcg atcttccgct tcatcgatat
     4861 cagaagcatc gattttcttt ttatttcttc tagcagctac taacgctgct tcattcaaga
     4921 cattttctaa atctgctccg gcaaaacctg gtgtctgttg agccacaact ttcaagtcaa
     4981 cgtcatctgc catcggttta ttccgtgcat ggactcgcaa gattgcttca cgacctttta
     5041 catctggacg tccaactaaa atctggcggt caaaacggcc tggacgtagt aacgctgggt
     5101 ctagtacatc agaacggttg gttgcagcaa tgacgattac tccttcattt ccgtcgaagc
     5161 catccatttc tacaagcaat tggttcaatg tttgttcgcg ttcatcatgt ccgccgccca
     5221 taccggcacc acgttgacga cctaccgcat caatttcatc aatgaaaatg atagctggtg
     5281 cgtttttctt cgctgtttca aacaaatcac gaacacgact tgctccaaca ccgacaaaca
     5341 tttctacgaa atcagaaccc gagatcgagt aaaacggtac tccagcttct ccggcaacag
     5401 cttttgctaa taaggtttta cctgttcctg gaggtccttc taaaagtaca cctgccggga
     5461 tacgggcacc taattcaaca aatcggcgag gatctttcag aaattcaaca acttcaacta
     5521 gttcttgttt ttcttcttcc gcacccgcta catcagaaaa acgtacgcga ttagcttttt
     5581 tgtctgcttc tttggctttt gatttaccaa agttcatgac acggccgcca ccgccaccgc
     5641 cgccgccttg ttgtcccatc atcatgtaaa agaaaaagat gatgattacg attggcaaga
     5701 agctaagcaa tagggaaacc catataccgc tgcttgattc ttcttttact gttgtagcaa
     5761 ctttgttttc ttttgccagt tctgttactt ggctcaatgt tgtatcattt ggcaaaatga
     5821 ttgttgtgaa atgggtagta gtcgtatctg ttgaacctaa gatagctaac ccaccgctgt
     5881 tagcgacggt ctgctgttct ttgtattcac cagtgatctt atatacacca tttgcaggct
     5941 gcacttcaaa ttctttgatc ttgcctgctt gtagttgttc tgtgaatttt gagtactcga
     6001 tatctgacga tcgctggccg ttattaccaa aaatgaaata aacgaccatt accatcgcta
     6061 agattacgag gacataatac aaggcatttt tcatcatgcc gttgttcttt ttgttcatag
     6121 ttgccctccc taagcgtcaa tttaatttaa cagtttttta tcaatcctga ccattctatt
     6181 gtaccataat ttaattgaga tacagcatta atttgattga taaacactag gtttcaatac
     6241 accgatgtac ggaaggttac gatatgcttc tgcataatcc aagccataac cgaccacaaa
     6301 ttcatttggt acgttgaagc caacataatc tgcttcgata tttactactc ttccttcagg
     6361 tttgtcaagt agtgtaacaa ttttgactga ttttgctttg cggtatttga aaagatccac
     6421 taaataagca agtgttcttc cgctgtcaat gatatcttca acgatcaaca gatcgcgacc
     6481 ttctacattc gtatctaagt ctttgacgat ttttacttcc cctgacgaga ctgttgcatt
     6541 cccgtagctt gaaacatcca tgaaatccaa ttccaaatac gtatcgatag aacgaacgat
     6601 atctgccatg aatggtaccg ctccttttaa gacacctact actaggggat tggtgttttg
     6661 gtaaatttct gttaactctt tgcctagttc tttgcaacga acttgtatct cttcttgtga
     6721 gatcaaaatt ctctcaatat ccttttccaa catcaggttc tccttccaat tgcttctttt
     6781 tgatatttgt aaagaagtat gtagtgtatt ttatcagttt cttcacgaat actcaaatat
     6841 gaaaaggcaa aaggtatcaa gctatatata tttcttttgg catctgtaat gatccaagca
     6901 tgctgccttt gtgaattagg tactttctga tcgataaaat atctagatac tttttttgtg
     6961 agattctctg ttaatttaat gcggtctcct gcttttcttt tttcgacata cagttgcaca
     7021 tcagcaggaa tccaaatgtc atgagaaaat agagaccatg aatctgttgc tttgatgtcc
     7081 cctgcatttt caggtttgac cagaccaatc cattctgttt cattcaaata tattccttgt
     7141 tctggaatca gcaacaagcg ctggtcttct gtctggctag ttgcttgttt ttctttttta
     7201 gagagtacaa caaaatgata ctctcttttc agtatccaat tattgccaat actgagttgc
     7261 cattggcttt ttttctgttg ccactggtct aaaatcgact ccatttgttg ttctttcaaa
     7321 ataattcctg tttttgagaa tgctttgtcg aaaaaggagt aaaaaaaata gtatcgttca
     7381 gcatctgtca tttcttcaat catttctgca gaaaatcgaa attgttcctc ccactgttcg
     7441 acttcctttt cgatcaactg tcccatatat ttttgaatga cttggtctgc ccattggatc
     7501 tgttgcgaaa attgctggaa atgtctcaac gcctgtgtat tttcttgttt caattgagga
     7561 actaccaaat gacgaagccg atttctctgg atatctaaca attggtttgt ctgatcctca
     7621 aagtaaacaa gctgcgattc cgctgcataa gtatataggt cttctttgct aaaactcagc
     7681 aacggacgaa tcagtctgcc cgtagcaaat ggttgtactt ctttgattcc agaaaaagta
     7741 tttaactgtc ccccacgaac gattttcatt aacaccgtct cgatttgatc atctccgtga
     7801 tgagcagtca tcaacgtgtc atattgtcct tcttccatca cgcgagtgaa ttcttgataa
     7861 cgaaagttac gagcttctgt ttctatggcg gatttggctg gatcttccca aatacttaca
     7921 tataaaggga gattcttctc ttcacaatat gagcgaagat aggcagcttc ttctgctgat
     7981 tcttctctta attggtgatt gatgtggacg atacctaaag aaaaaccgat ttggcgttgc
     8041 acttgtaaaa gtaaatggag caaaaccatc gaatcaacac cacctgatac cgctgctaaa
     8101 attctcgatc cttgttgcca gaatccttgt tcattcccgt gccgaataaa tgactgctgt
     8161 aatagcggtg cttttttccc gcctttttca acgttcatcc tttccacacc ctttcttcct
     8221 aaaaaaaaga gagcgggaaa acgtctaaaa cgacaattaa cctgccctct gttgatacta
     8281 tgacccaaat agtcgcacta tcattatttg cggcttttta gctgcgacgt ccgccacggc
     8341 caccgcgctt tccttctgtg tttcgcttca atgaagataa gcggtcatcg ctatctttca
     8401 aaaatgaact catcaatgaa tcgaagtctt ctttttgagc tgaaggttga ctgcgagaaa
     8461 aagatttttt ctgtgctgga cgatttcggt tgtcacgggg atctttttgt tgatattttt
     8521 taggacggct ttcttctcca gattcagcag gttgttcctg cgctttccgg atagatagtc
     8581 cgatttttcc atcatcattt actgaaataa ctttaactgt tacttcatct cctacactta
     8641 aaacatcatg gatgtctttt acatagccat ttgacacttc gctaatatga actagacccg
     8701 tttttccagc acctagatca ataaacgcac caaaatttgt gattcctgat acttttcctt
     8761 gcagttttgc tcctacctcg attgacataa aaaaaatgtt cctcctaatt atttcccttt
     8821 tttatattcc tgcttatttt gcagttgtag aactagttga ttgatctgtt tcatttgtcg
     8881 agctgtctgt atttgattct aatataggat aaactttttc accgtctttt gagtaaaaga
     8941 atttgcttct ggcaagttta gctacatact catcatcttt cagcaatgaa acatcttggt
     9001 gcagctgatc aacgtctgcg ttgacttctt tcatttcagt atttgcttcg actctcaatt
     9061 cgttcaattg atgcatcctt tgcatgtcat ggaaaatctg cacacctatt atcgcgaaaa
     9121 tgaccaaagc accaacaaaa attgcagcca gtctgcgtct tcgaaaaatc agttgacgtt
     9181 gttgttgctg aaattcagca tactttttct ttgcatattc cgtatctagc gcagcgattt
     9241 tattgctttt tttcgtcact tggattcgct ccaatctttt tgattgtctt tttcattata
     9301 ccaatatcgt gaaagcttgt ctaatctatt tctctagtgt ttctgtgcga ttttcagaaa
     9361 taatttcgta cattttttgt gcatcttctt ttttggttga ttcatgtaat tccgtaattt
     9421 tcacttccaa cgttttgttg ccaaactgga ctttcaaaat atcgcctact ttgacatcac
     9481 tggaagattt cgccaattta ccgttgattt gaattcttcc tttatcagct acttcttttg
     9541 ctactgatcg tcttttgatg attcgggaaa tttttaaaaa tttatctaat cgcatatctt
     9601 attcctcact ttttaatttt tttatttaat cgcaggattt tcttaccaaa aggcaacatt
     9661 agccattctc gtactgtaaa cagacgaaga cgaatcactg cataaatgaa cacagaagcc
     9721 ccgatcccaa ctcctatcac gctgacaaaa aaagcagtgg aacgatgagt tacaggacca
     9781 aatactatat tcaatacacc ataatacagc agcagtacta ctgtcataca aagaaggctg
     9841 aacatcaact tccgtccaaa atgccgttct ttccaaaaag catttagctc ttttgtttca
     9901 attcgaacaa aataccaaag cgtcacacct aatcctaaaa gggtagacag gcttgcacct
     9961 gctgttccta ataaaccggt cagaaaagaa gtagtcaatc cttttaccaa tagtccccat
    10021 cctgcgcctt ttaaagaagg gcgaaacgag ttcttgcttt gagcaatact ttgataagct
    10081 tggatgactg cagtgaaagc aatggaaaaa acaaataata caagcgctgc atttcccgca
    10141 taatctttaa acaacgcata attgatataa gggagcagca aggctaatcc caaagacgca
    10201 gctaatgcta aagcggtcgt caatctaaga tagattttgg ctgtctgtaa aaactgacga
    10261 tagatccggt tggtcaaatg acgagtcaac gcaggcagga atgtcgcact taatgcagtg
    10321 gcaatcacta aacctaattg tactaaaggc tgacctcgat catagacccc ttttgctatc
    10381 ttcgcaccgt attctgacag tccgtaaact tccaaagcat tttttacaaa gaaagagtcg
    10441 atcagctgga aaaagataag caatccacta taaatcgaaa ccagtccgcc ttctaccaga
    10501 aaacgccgga tcaagggctt tgacggctgt ttgaacaatg gaaaccgtcg gaagcttaac
    10561 gctcctccat gaatcttttg ttcatagtag tgaaggatcc catatgcgca taaaccgcca
    10621 aaaacagctc cactcattgc tgccgtccct gtttggtaca ccgtccatcc aagctttcta
    10681 aaagcaaacg cactacagac aatcacaaaa acacgaacga actgctcaac tacttgagaa
    10741 acagcagttg gttccatcaa gaatcttcct tggaaattcc ctcgataaaa agatagacca
    10801 ggcatcagca aaaaggtaaa ggaaactact tggatcagcg gtttcaattg tatgtctccc
    10861 atcatccaag caatccagct gctaaatgca aagacaaaca accacaaaaa gacacctgtc
    10921 caaaatacta agggataaag attttgcaaa gcttgctttc gctcgaaagg attctttctt
    10981 tctgcaacgt actttgatat gaactgcgga agaccggata aagctaaagt cattgctatc
    11041 ccataaattg gataaacttg ctgataaaca taaaaacctt cgtcgccgac taagttttgg
    11101 aggggaattc ggtaaagggc acttaatact tttgctatga aagaagcaat cgtcaataca
    11161 aatgctcctt gcatgacgcg ccgcatttct ttatgatcca aatcattgct ccttcctacg
    11221 ctgacagata tttttgctca cgcaacgctt tgacaaactt ttggatttcc tgaagccata
    11281 ctgcttcttc catttggttg gggatcgtca agcgtatcac catctgttct ttttcaacgc
    11341 ctagactggc ttttagtttt gtcgctgtca gtgcttcgaa taactgttcc acactatagc
    11401 gttttgttcc aactttactt agagtaaaga ctatatgcgc ctcctgtttc cttatcgttt
    11461 ctaggagcgc tcgatcgcca tccattttga tctgcccaat tgtcaagaga tgtgctactt
    11521 cttctggata ttctccaaaa cggtcgagca gatcatcttc tagttcatct agcatttctt
    11581 gcgaatcaag ttcacgaatt cgtttataga tctcgatttt ctgccgttga tctgccacgt
    11641 atgtttcagg tagatacgca tcgacaccga ggtcgatctc aacagtggtc ttctccactt
    11701 gactattctt tccttgttta cgagaaacag cttcgcttag catttgagaa tacatatcga
    11761 aaccgactgc atcaataaat ccatgctgct gagcacctaa gagattacct gcaccgcgaa
    11821 tggaaagatc ccgcatcgca atcttgaatc cagatcccag ttctgtaaaa tctttgattg
    11881 cctgaagacg tttttcactg acttcgttaa ggatcttctg ctgctcatac ataaagtaag
    11941 catatgcgac acggctgctt cttcctaccc gccctcgcaa ttggtataac gtggataatc
    12001 ccatataatc agcgttctca acgaacaacg tatttgcgtt cgggatatct actcctgttt
    12061 caatgatggt tgtagtgaca agaacatcat attgaccttc aataaattca aaaagcgtat
    12121 tttctaattg cgcctctgtc atttgcccat gagcataacc gatccgtgct tccggaacga
    12181 gttcttggat ttcttctact ttttgttcga tcgtttctac ccgattatat aaatagaata
    12241 cttgtccacc tcgtcccatt tctcgatgga ttgcttcgcg aatagcccca ggattttttt
    12301 ccataacata cgtttggatc ggataacgat tcgcaggcgg tgtttcgatt actgaaagat
    12361 cccgcacgcc taacatcgac atatgcaaag ttctcggaat cggtgttgca gtcagagtca
    12421 aaacatccac ttgtgcacgt aactgtttca atcgttcctt atgttttaca ccaaaacgtt
    12481 gttcttcgtc aatgatcagt agacccaaat cgctaaattc aatatctttt gacaaaattc
    12541 ggtgggttcc tacaacaata tcaacttgac ctgtacgcag ttgttcgatg gtttcttttt
    12601 gttgctttct tgttctgaaa cgacttaata gcccgatatt tacggggaaa ccttcaaatc
    12661 tttccagcat cgtttcatag tgctgctgtg ccaagatcgt tgttggaact aaaaaggcta
    12721 cttgtttgct ttcctttatc gctttaaagg ccgctcgaag cgctacttct gttttcccat
    12781 aaccaacatc acctacgagt agtcgatcca ttggtttttc tttttccata tcccgtttga
    12841 tctccgctgc acttctcaac tggtcatcag tttcagagta ggggaaggca ttttcgaatt
    12901 ctttttggta ggcgtcatca ggtccaaaag catatccttt ttctgactct cttgcggcat
    12961 ataatttgat tagatcgtca gcgatatcct cgatttttga agagactttt cgcttggttt
    13021 tcgtccattc acttccgcct agcttattga tgcgaggcgc tttggcttca gaggcgacat
    13081 atttttggat cagattcagt tgagtaacag gaataaacag tttgtcgtcg ttttgataca
    13141 aaatagtcat atagtcttgg tgaacgccat ctacttctag cgtttccatc ccaatgtatt
    13201 tcccgatacc atgattggca tggacaacgt aatctcctgc ttttaactca ttatagcttt
    13261 tcaatcgctc tgcgtttgtt actgtttgac gtctcgtttg ttttttcgtc gttttttgga
    13321 aaatttcttt ttctgtgata gctactattt tttcttgagg aagttcaaaa ccagcctgca
    13381 aagctccttc aactaattgt acctgtcctc tgatcaatgc atttttttct gtttctacta
    13441 ctaatatgtc ttcatctcga agcatttgtt ctgctttgcg tattctgtcc tttgtcggaa
    13501 taaagatcac aacagtctgt ttttgcttgc gccaacggtc catttctgtt ttcagcaatg
    13561 gcatttgtcc gaagaactgt tgcatcgtcc gatattgaaa attatggatt gcttggaaac
    13621 gaagatttcc catccctttt tggaagagtg agaaaaaggt tgtggtgaac ttttccttct
    13681 gcacgatttt ttgtatatct gctgaaaatg tctgctcaga aaacacccgc aattcgctga
    13741 ttttttgagt ctgccattct gccgcttccc tttcgatttc ccgatttgtt tccatgattc
    13801 gctgataatc atcaacgact agtaaactat ttgaagggaa ataatccaag atcgtcacag
    13861 attcttggta aagaagatct gagtagaatt ttgcttcttc tgtcgggaca cctgcttgcc
    13921 agcttgaagt cagctgaccg aagtaatctt gcaaaaactc gcggtctgct tcttctgtgg
    13981 tcgcagcaag ccgtttttct agcatttttt gcatttgctc tattccatgt tctagatcct
    14041 ctggtccgta gacttgttca ctcgttgggg aaacccagat ttcttccgtt ttttcgattg
    14101 agcgttgtgt ttctgcttca aaataccgca tagaatctat ctctacatca aatagttcta
    14161 cacgaacagg ataggttgtc gttaacgggt aaatatcgat aatgcttcct cgaatactga
    14221 attctcccgg ttttgcgacc atctcttctc gtacatagcc catcaaagtc aattggcgag
    14281 gaagattttc cacgtctatc tcatcaccaa tatgccaatt caattgcgcc tgtagccaag
    14341 ttttcttatt cggcagtcgt ttgtgcaaag atgcgactgg caacaaatag atacctgctt
    14401 ttttttcttg aagtgcgtta agcgttgcaa ctctctcgcc tcgcgcttct ggagaagaaa
    14461 aggccatttc agcagaaagc acttcatcca ctggaaataa atggatctga tcttgcggaa
    14521 tcacatgttg cagatcttct gctaactgat tcgcataata caaatttggc atcagaataa
    14581 tcatgttttt atttgttttc ttaaacgctc gagcaaatag aagtgttttc gctgagcccg
    14641 ctaatcctgt gattagttgt ctaggcattt tttcttctaa attgctgacc cattctttta
    14701 cttgcggatc ttttccgact aattgaataa tatccattcc ttttcctcct tcctcaattg
    14761 aatcgattca tcgcatccac gaacgaatgc ccttctatga gatattcgac tgctttaaca
    14821 gattgacaca tgctttcttc tatcaaaggc tgattttctt tgctgaaagg actaagcaca
    14881 tgctggacta ctgttttttt cccttctggc ctgccgattc ccaccttgat tcgatcaaag
    14941 acattcgtat tcaaatggga aataatactt ttgataccgt tatgcccgcc ggcacttccc
    15001 ttttgacgga gccggatttt tcccactgcc agatccaaat catcgtagat tacgactaat
    15061 tcctctggat aaatcccaaa ataagtcatt agtgggccga ctgcacgacc agattcattc
    15121 atgaatgttt gtggtttgac taaaaggatc ttttctccat tatgaaaaaa ttcggcaact
    15181 tctgcttcga acggattttt tttgaaagta gcttgatgct cgcgtgcaat tttatccacg
    15241 accataaagc cgatattgtg ttttgtatat tggtatttgg tacctgggtt tcctaacccg
    15301 acgatcattt tcatttttgt tttccaccta attctaaaaa gcagcacaaa aggctggact
    15361 gctaattagc ggtccagtcc atcgagcttt tttctttttt acacttgtca atgcatagta
    15421 taacacaaaa caaggcgttt cggctaaaag agaatcgtta ataaatgata aagaaattaa
    15481 aaatttcttc aaagtttaat gaaataattg atatataaaa atagtgaaat aacgcacatg
    15541 taagctttta catataatac aaccttgtta tctgttatat tttatagcca aatagcgtga
    15601 catggggctt gagaaatgtt agcataagca tgggaaaaaa caaatcaatg gaaggattga
    15661 tactaaatga ctgcagctgc agaaaaaaaa gatcatcaaa aagttattct tgttggagac
    15721 ggcgccgtag gttctagcta tgcctttgct ctagttactc aaaatatcgc tcaagaagta
    15781 ggtattgtgg atatcaatac tgctaaaact gaaggagacg caattgactt gtctcacgct
    15841 ttggcattta cttcacctaa aaaaatctat tctgctactt acgcagacgc acatgatgct
    15901 gacttagttg ttattacagc tggcgcacca caaaaaccag gtgaaactcg tttagactta
    15961 gtaaacaaaa acctaaaaat caaccgcgat gttgtcacac aaatcgttga atcaggattt
    16021 aacggtatct ttttagttgc cgctaaccca gtagatattt tgacttactc aacttggaaa
    16081 ttctcaggtt tccctaaaga acgcgtaatc ggttcaggta cctctcttga ctcagctcgt
    16141 ttccgtcaag cacttgcgga attagttgac gttgatgcac gtaacgtcca tgcttacatc
    16201 ttaggtgaac acggagattc agaattccca gtttggtcac atgcaaatgt tgccggcttg
    16261 caaatctatg aatgggtaaa aaacaaccca gaaatcgatg aagaagctat ggttaaccta
    16321 ttctttggcg tacgtgacgc tgcttacaca attatcgaga aaaaaggcgc tactttctat
    16381 ggtatcgcag tagcattagc acgtatcact cgtgccatct tagatgatga aaatgctgtt
    16441 cttcctttat cagtattcat gaatggcgaa tatggattga acgacatcta cattggtgca
    16501 cctgctgtta tcaaccgtca aggtatccaa aaagtaatcg aaattccttt aagtgacagc
    16561 gagcaagacc gtatggctgc ttctgctaag caattaaaag aaattctaga tgaagctttt
    16621 gctaaactag acgctgaata atctttgaac atgacggaaa acatctattc ggtgttttcc
    16681 gtttttttat tttatcaccc ttatggtttc taaatttttt cataaagtag agataacctt
    16741 cgtcttttcc tctaaattat gctatcatga gaccgttcca ccctaaactt ttacttaagg
    16801 agttgtcgat atgacaagat cttcgcatcg aaaaaaatcg aaacatgttg ctttgctttc
    16861 agttcttggt gttctcttag tgctgatcag cttttatacc tatcgcagta catactacac
    16921 aaagcatttt ttgccgaata cagaaataaa tggaataaat gtcagcaatc taacagtcga
    16981 aaaagcaaat gaaaaattaa aagaagctta ttctgataaa aaactggcta ttaaagaaga
    17041 tggaaaagtc tggcaggaag taccaaaatc cgagctcggc tacaaagatg atttcacttc
    17101 ggagctttct ccgatcctaa atgaacaaaa cggatggact tggggaatga cttatgtctc
    17161 agcggcagaa aaacaagaaa tagatcctgt cagtcaaaac caccaaaaac tagacactac
    17221 cgttcagaca ttgacaacta agctgactga tttgaacaaa gaccggacac ctactactga
    17281 tgcaacgatc gaaaaagcag gtgactcttt cgttatcaag cctgaagtca atggaaatac
    17341 gatcgacgtc gatgctgcgg tcaaacaatt aaaaagtgcg gtaaactctg gtaaagatac
    17401 aattgaattg actgaattca aagaaaaacc aaaagtaaca tctgaagaca gctcactgaa
    17461 agaacaactc gcatccatga atgctatcgc aaatgtaaaa gctacttaca gtataaacgg
    17521 tgagactttc cagatcccat caagtgatat tatgagttgg ctgacctaca atgatggaaa
    17581 agtcgattta gatacagaac aagtacgtca atatgtaaca gatttaggca caaagtataa
    17641 cacaagtacg aatgacacaa aatttaaaag tacaaaaaga ggagaagtta cggtcccagt
    17701 tggcacttac agttggacga tccaaacaga cagtgagaca gaagcattga aaaaagcgat
    17761 tctggcaggc caagatttta ctcgttcacc cattgtgcaa ggtggcacaa ccgctgatca
    17821 tccattgatc gaagatacgt atatcgaagt tgatctggag aaccagcata tgtggtatta
    17881 caaagacgga aaagtggccc ttgaaacaga tatcgtttcc ggaaagccaa ctacacctac
    17941 acctgctgga gtcttctatg tttggaataa agaagaagat gcaactttaa aaggaacaaa
    18001 tgacgatgga acaccttacg agagtccggt aaactactgg atgccgattg actggacagg
    18061 tgttggtatc cacgattcag actggcaacc tgaatacggt ggagacttat ggaaaacccg
    18121 cggttcacac ggctgcatca acaccccacc aagtgtaatg aaggaattat tcgggatggt
    18181 cgaaaaagga acgcctgtat tggtcttcta acaaaaataa tatataaaaa gaaagcaccc
    18241 gtacgattgg tacaggtgct ttctttttat tatctaacaa tgtgctactt acatttcagc
    18301 agcagtaaag acttctgaaa catcatcatc gtcttctagc ttatctacta gacgttccaa
    18361 ttgctctttt tgttcctcat ttaaatcaat cgtcgtttgt ggaatcatcg tcaattctgc
    18421 ttgcgccaaa gtgaactctt tttccaactc atctcgtaca gcagcaaaat cttctggtgc
    18481 agtataaatc tcgaatactt caggagaagt ttgcagatct tctgcccccg cctctaatac
    18541 atcctcaaac atcgtctctt catcaacagt caacccatcg cgttcaatag cgatatatcc
    18601 tttgcggtca aacaagtaac tgactgaacc cgtttctccc agtgatccac cgtttcgagt
    18661 aaaagctact cggacatttg tcgctgtccg attacggtta tctgtcaatg catggactaa
    18721 gattcctact ccacccggac catatccttc atacgtgatt tcatcgtaat tcgtttcatc
    18781 tacggcactc gtagcctttt taatcgcccg ttccacattg tcgttaggca tatttgcagc
    18841 ttttgctttg tccacaacaa gccgcaaagc tggattcata gatggatcag gaccgcctgc
    18901 tttagctgcc atataaattt cacgagaaag tttctgaaag actttccctc gttttgcatc
    18961 ttgggcattt tttcttcctt ggatattatg ccatttacta tgtcctgaca tgatttttcc
    19021 tcccttgttc ttttgagaat acacttctat tctagtacta tttcacaaaa aaacaaagcc
    19081 acagccaatc tataaacgct tcttgaatgc cgcaaaaagc acacataata tcacagcagt
    19141 cagagcaccg atcgaatcca acatgacatc ttggaacata ggtgttcgtc caccggtcat
    19201 catctgatgg aattcgtcca aacctgcgta ccctgttgct gaaagccaag caaggatcgc
    19261 tgtcagccac caaattttta gcttggggaa aacacctaaa aacaagctgc ctcccaaaat
    19321 aaaataggta ccgaaatgtg cgcccttcct taaaaagaac tctaaaaatt tggcgtagcc
    19381 cagatggttg atactcacca tacttccacc ataattaaaa cgtatacccg ctaatgtgtc
    19441 tttaaatggc tgattaggca gccagctttc taatcgcgaa acttgtgatt gctgtccgta
    19501 agtttgagaa gaactgtaaa ataagattat aacgactatc agtgcgatta ctaaataaat
    19561 atttccgttt ttcatttgtt tttttgaaga attcataagt cacctctgta ttttatctta
    19621 gacttttttt gaagaaacaa caatcatttt catagagact tcttccataa aaaagtgcta
    19681 tacttaattt tgtattacaa tatgaaggga gttttttcat gagttattca ttaaattgga
    19741 atctagattc tattttttcc ggaggcagtc attctgatgc tttgaaccaa cggatgaagc
    19801 aattggaaga ccaaatgaac gagtattatc accgcgtaac aaaatggtca cctagttctg
    19861 acaatgcaga gcagttaaac gctattcttc aattgcaaga aacgattacg aatggtttta
    19921 cccaatgcag cagctatatt actgcattgc tttctgccaa tgtcaatgat tcagatgcaa
    19981 aggtcttatc aggcaagctt tacgcaatgc tgcctcgtct gcaatcagcc gaaactgtat
    20041 tgtcaaagaa atttgcagaa atatctgaca atgattggaa tcagatgctt tctcaatctt
    20101 cgtttgaaac aattgctttt cgtttaaacg agattcgtcg tgatggctca caattattat
    20161 cagaagctga agaaaacatt ataaatacgc tttctttaga tggtctaaat gcttggagca
    20221 gtcattatga tacgattgta gccagcattt cgattccctt cgaacaagat ggacaagtag
    20281 tcgaattatc tgctggacaa gccttcaata agatgatggg cgatccagat ccaaaagtca
    20341 gagaaacact tttcgcagca tgggaagaag cctggaaaga aaaggcaccc ctcttcacgg
    20401 atactttgaa tcatttagat ggttttagac tttctgacta tgaacttcat ggtgtcacag
    20461 attttctaca aaaaccatta gagtacaatc ggttgaagaa agaaacattg tctgtcatgt
    20521 gggatacaat ccagaaaaac aaacaacccc ttgttaatta cttgactaga aaagctaatc
    20581 ttttcggtaa agaaaaaatg gaatggcaag atcaagatgc acctattatt ttaggcgatt
    20641 tgaaagaaaa aacctttagt tttgacgaag cagcagcatt tattatcgaa aactttaaca
    20701 aattcagtcc aaaaatggct aaatttgccc aatctgcttt tgaaaagagc tggatcgaag
    20761 cagaagatcg tcctgggaaa cgtcctggtg gttattgtac tgagttacca gaaacaaaag
    20821 aatcaaggat ctttatgacc tacagtaatt ctgttaatga ggttgcgaca cttgctcatg
    20881 aactaggaca tgctttccat agcagcacga tgtgggattt accttcttta aatcgcgaat
    20941 atgccatgaa tgtcgcagaa acagcaagta cattcgcaga attgatcgta gccgatgcta
    21001 cattaaaagt cgcaaaaaca aaagaagaaa aaatcaattt gctagataca aaactgcaaa
    21061 atgctttagc tatgttcatg aatatccatt cccgattcat ttttgaaaat cgattctatg
    21121 aagcacggca agaaggatta gtctcagaag ataagatcac agagatgatg gttgaggcgc
    21181 aaaaagaagg ctatcaagga gctttagcta cttaccatcc atatttctgg gctgcaaaat
    21241 tgcatttctt tattgatgac gtaccgtttt ataacttccc atacacattt ggctacctct
    21301 ttagtttggg catctatgct tacgccaacc aaaagggagc aaattttgaa gaagaatata
    21361 tccatttact acgcgatact gcctcaatga cgacggaaga tcttgcgaag aaacatttga
    21421 atgtcgatct tacccaacca gatttttggc aagcaggtat cgacatggtg actgaagata
    21481 tccaaacgtt catggaattg acagaagaat ttatctgatt tctattttat cacaataaaa
    21541 aaagagttag tccagaatgg gctaactctt tttg
//
LOCUS       DAZBSA010000047        20737 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000047 DAZBSA010000000
VERSION     DAZBSA010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.047"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..1581)
                     /gene="ahpF"
                     /locus_tag="ACHGMT_001576"
     CDS             complement(52..1581)
                     /gene="ahpF"
                     /locus_tag="ACHGMT_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFN6835321.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1591..2154)
                     /gene="ahpC"
                     /locus_tag="ACHGMT_001577"
     CDS             complement(1591..2154)
                     /gene="ahpC"
                     /locus_tag="ACHGMT_001577"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFN6835322.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2298..2960)
                     /locus_tag="ACHGMT_001578"
     CDS             complement(2298..2960)
                     /locus_tag="ACHGMT_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN6835323.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3144..4790
                     /locus_tag="ACHGMT_001579"
     CDS             3144..4790
                     /locus_tag="ACHGMT_001579"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFN6835324.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4781..5863
                     /gene="uxuA"
                     /locus_tag="ACHGMT_001580"
     CDS             4781..5863
                     /gene="uxuA"
                     /locus_tag="ACHGMT_001580"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFN6835325.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(5971..7524)
                     /locus_tag="ACHGMT_001581"
                     /pseudo
     CDS             complement(5971..7524)
                     /locus_tag="ACHGMT_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7672..8813
                     /locus_tag="ACHGMT_001582"
                     /pseudo
     CDS             7672..8813
                     /locus_tag="ACHGMT_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(8939..10018)
                     /locus_tag="ACHGMT_001583"
     CDS             complement(8939..10018)
                     /locus_tag="ACHGMT_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFN6835326.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(10121..10561)
                     /locus_tag="ACHGMT_001584"
     CDS             complement(10121..10561)
                     /locus_tag="ACHGMT_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFN6835327.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(10618..11028)
                     /locus_tag="ACHGMT_001585"
     CDS             complement(10618..11028)
                     /locus_tag="ACHGMT_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFN6835328.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(11208..11642)
                     /locus_tag="ACHGMT_001586"
     CDS             complement(11208..11642)
                     /locus_tag="ACHGMT_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN6835329.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENRSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(11742..12590)
                     /locus_tag="ACHGMT_001587"
     CDS             complement(11742..12590)
                     /locus_tag="ACHGMT_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN6835330.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            12979..14745
                     /locus_tag="ACHGMT_001588"
     CDS             12979..14745
                     /locus_tag="ACHGMT_001588"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFN6835331.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            14847..16106
                     /locus_tag="ACHGMT_001589"
     CDS             14847..16106
                     /locus_tag="ACHGMT_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFN6835332.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            16253..17554
                     /locus_tag="ACHGMT_001590"
     CDS             16253..17554
                     /locus_tag="ACHGMT_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6835333.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            17557..18408
                     /locus_tag="ACHGMT_001591"
     CDS             17557..18408
                     /locus_tag="ACHGMT_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFN6835334.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            18421..19236
                     /locus_tag="ACHGMT_001592"
     CDS             18421..19236
                     /locus_tag="ACHGMT_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFN6835335.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            19387..20553
                     /locus_tag="ACHGMT_001593"
     CDS             19387..20553
                     /locus_tag="ACHGMT_001593"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN6835336.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT         6868 a         3745 c         3841 g         6283 t
ORIGIN      
        1 tcttgtcacc atcctttttt ccgctctctg actttttaca atcttcatag ctcaagtgcg
       61 catcagataa tcgaatgctc ctaaagctgc agtagctcct gaacccattg aaacaatgat
      121 ttgtttgtat ggactatctg tacaatcacc agctgcgaaa acgccgggga tattcgttgc
      181 gccatttttc tcggtcacta tttcatttct tggattcatt tcgatcgttc catctagcca
      241 atccgtatta gggagtaagc cgactagaat gaatactcct gaaacagcta gtgtatgcat
      301 ctccatcgta cggcgatcga tataggagag agattctacc tgattcgtac cgctgatttc
      361 tttcgtcgct gcatgtttga taacagtgac attcgcaaga gagactagtt tatcctgtaa
      421 gatttgatct gcttttaatt ctggtaagaa ttctagaaca tatacatgtt ttgctaaccc
      481 agccaaatcg attgctgctt caacaccaga attaccacca ccgataacag caatctcttt
      541 tcctttaaat aaagggccgt cacagtgtgg gcaataagca attcctttat ttttaaactc
      601 tttttcgcct ggtacattga tagaacgcca gtgcgcacct gtcgcgatga tggcggtttt
      661 tgctttaagt gatataccgt tagctaagcc aatattaata aaatgttgct ctttatttaa
      721 ggaaacggcc tgttgattca tcataatgtc gatagggtaa ctacgcacat gactttctac
      781 ttgctgcatc aattttggac cttctgtgta agaggtacca atcatatttt caatccctag
      841 tgtttcaacg acttgtccgc cgaaagaatc agcgacaagt cctgtacgga ttcctttacg
      901 tgctgcatag atagctgcac tactagctgc tggtccaccg ccaatcacta atacatcata
      961 aggttctttt tcaaacaact cttcttcaat caaagggcca gtgatttttt caacaatttg
     1021 ttcgatcgtc atgcgtccgc tactaaattc ttctccattt aaaaacacag ttggcactgc
     1081 catgattttt ttatcatcga tttctttttg gaacatccca ccttcaatca tcgtatgagt
     1141 aatatttgga tttaatacag ctaatatatt caatgcctgt acaacgtcgg ggcaattatg
     1201 acaagtcaag ctaacataac tgtcaaaatg caatgtttgt gttatttttt tgattttaag
     1261 actcaaagat tcactgattt tcggtgcccg tccactaatt tgtaacatag ccagtaaaaa
     1321 agaggaaaat tcatgaccga gggggatacc agcaaaaacg attccacttt ctttgccaac
     1381 tggattgata gaaaaactag gtgtgcgcat cgaatctctt ttttcccaag agattttagg
     1441 agacatttct gctatttcct tcgtaaattc agctagtttt tgactatttt cactattatc
     1501 caaactaaca gaaaaaacaa taggagattc aatcagtgct aaatattgtt cgagattttg
     1561 tttagtttct ttgtctaaca tatccaccac ctaaatcttt ccaacaagat caaaacttgg
     1621 tttcaatgtt tcgccacttt ctttccattt tgccggacaa acttcacctg ggtgggtacg
     1681 tacatattga gcagcgcgaa tcttatcaat cagtgcacta gcgtcacgac cgattccatc
     1741 tgcattgatt tccatagctt gaacgattcc gtctggatca ataataaatg ttcctcgctg
     1801 agctaatcct tgctcttcat ccaaaacatc aaaaatacga gaaatctgat gagacggatc
     1861 accgatcatc acgtattcga ttttaccgat agcatctgat gtatcatgcc atgctttgtg
     1921 agtaaagtgg gtatcggtag agcaagaaaa gacttcaaca cctaaagatt tcaatgtact
     1981 gtattgttct tgtaagtctt ctaattcagt aggacaaaca aaagtaaaat cagctggata
     2041 gaagcaaaga atactccagt tccccataag atctttttca ctgacttcta aaaattctcc
     2101 ttgatgataa gcatgtgcat taaattcttc gatttttttc ccaatcaatg acatagtaaa
     2161 acctccaata ataattattt atttataatt attgtaattt agttggagtt gcaaagtcaa
     2221 tggatatttt gttatttaat aaagattact caaaaacgaa ggttaattat aaaaatagaa
     2281 gaggtcgcaa gaaaatgcta ctgctggaaa tactcgggat aacgttgagt aacagtttcc
     2341 tgttctttca gcatcatgtg taagtgcaat cccgtttgaa atcgagcttc ttctggtttt
     2401 ttatcaacta aagccttgag taaatcataa tgttgttcca tgatcgtttc ccatggaagc
     2461 ccctgaatgg tcactcgtaa ccagcggaaa cgttctaaat gggtattatg gtcatctaac
     2521 cagttccaaa cttcttttct tcctgcaatt tcgaaacaag cttcatgaaa taaattatct
     2581 gcacggaaaa aagcgcgttc gtcatttatt ttgactgctt tttcttgttc ttctaaaatc
     2641 gtttctagca ctattcgtgt ttgtgtgtcc aatttggaac aacattcaat catgatttgc
     2701 tcttccaact gttctcggac aaagcgagca ttttctgcag aaacaagatt aattttggaa
     2761 acatacgtac cactttgagg tatcgtatac acgagttctt gctgacgtag ttgaatcaat
     2821 gcttcgcgaa tgggagtccg gccgatagca agatgttcct ccagtgcctt ttcagatatt
     2881 ttccttccag gttctaagtc agcatagata atttgtttac gaatcgcttg ataagcttgt
     2941 gattgcaagc tgcttttcat ctttattcct ccaaaatcat ttctatgaac gtagtataac
     3001 agaagaggtc tgaaaaacaa tctggtatgt cacattaact agtaaatttg tataaatagt
     3061 aaaattttgg ttgacatagc gatttttatt gcataaaatt cacttgaata caaaaactaa
     3121 tatgttagtt taggagggga aaaatgattg atttaaacaa ctggaggaac tttatccctg
     3181 aattatctca aaaagaagta gaaatacctc attttgaccg tgcatcatta agagaaaatg
     3241 gacaaaaaaa tcctgtctgg atccatttcg gtggcggaaa tctttatcga ggatttcatg
     3301 cagaaatcgc tcaacgttta gcggatctag gcgaattgtc cactggtgtc attgtttgtg
     3361 aaacattcga tgaagaaata attgaaaggg cttaccaaga ttttgatgat ttgattcttg
     3421 aagtggtcat gccaagcagt ggagagttcg tcaaacgtat attagctgcg acagctgaca
     3481 gtctgtattg tcatccagat tgttctgaac actttgataa gatgattcga cactttgaaa
     3541 atcctagttt acaattagtt acttttgcta ttactgaaaa aggttatcga ttgaaagata
     3601 caaataataa atatatagga ccgattgcag tcgatataaa aaatggtcca aagaagccat
     3661 cacatacaat gagtattctt gctgtgctac tttataaacg ttttttatct ggagcttggc
     3721 cgattgcact tgtctctaca gataattttt cacaaaacgg tagtcaatta cgtacttctt
     3781 tgctaacgat cgcaaaagaa tggcaaaagg aaggttatgt acaaaataca tttatccaat
     3841 acttatcgaa tgaagaaatt gttgcgtttc catggacgat gattgatcgt atcaccccga
     3901 atccttcctt gtcagttagc aaaatgttgg caaaagaggg cttttcaaat atgtcgattt
     3961 tccaaaccgc aaaaggaaat aatattgctc catttgtcaa tacagaggac atccattatt
     4021 taatcatgga agatcatttt cccaatggac gtccgaaatt cgaaaaagct ggggtattct
     4081 tgactgatcg tgcaacggta gataaagcag atacgatgaa ggtgacgact tgccttaatc
     4141 ctttgcatac gtctttagct attatgggtt gtttactggg gtataggagc atagctgagg
     4201 agatgaaaga tccagattta tcgctgctag tttctgaaat cgggtacaat gaaggcctcc
     4261 cagttgtgga gtctccaggt atcattgatc caaagatctt tctggaagaa gtaattactg
     4321 tgcgcttttc taatccaaat attcctaata tgcctcagcg aatcgccacg gatacctctc
     4381 aaaaggtggc gattcgttat ggtgagacga ttaaaaaata tatggaaata gaagttaaag
     4441 acccgcagac acttgtattc ataccattga ctctcgctgc ttggcttcga taccttattg
     4501 gcattgatga tcaaggaaaa ccattcatcc cgagtccaga cccactattg acagaccttc
     4561 agcataagct ttccgatatt caactgggta cgcaagatac atcacatatt catgagaaaa
     4621 tggctcctat tttgtcaaat aaagaaattt ttggtgtaga tctttaccaa gcagagcttg
     4681 gcagaaaaat agaagaaatg ttcatcaaga tggtagcagg accaaatgcc gtacgaaaaa
     4741 cattacagga ttacttacaa gaacatggag gaaatttatc atggaaatga cttttagatg
     4801 gtacggatca caggaagaaa agattacctt agatgaaatc agacagattc ctgcggtcaa
     4861 agggattgta ggcacattga tggatatacc agtaggagag gtatggccta gggagaggat
     4921 caaagaacta aaagaagaaa ttgagtctcg tgggttaaca ctaaaagtaa ttgaaagcgt
     4981 caatgtgcat gatgccatta aaattggatt accttcccgt gagcagtata tcgaaaacta
     5041 taagcagaca atccgaaact tggcagaata tggaatagaa gtcatctgct ataattttat
     5101 gcctgtattt gactgggtga aatctgacct tgactaccgc ttagaagacg gctcatccac
     5161 gttagctttt atatctgatg atattccaga tgatccaaga gaaatcgtcg cgcgcatcga
     5221 acattccagc aatgaatttg gattgccggg atgggaacct gagcgcttag ctcctatcaa
     5281 atccttgctt gaagcctatg catcagtaga cgaagaaaaa cttcgagaaa attttgccta
     5341 ttttctccaa tcaatcatcc caacatgtga agaggtaggg gtcaaaatgg ctgtacatcc
     5401 agatgatccg ccatactcga tttttggatt agctcgaatc gtaaagaatc gagaagattt
     5461 agattggttg tgtcgcatag tagacagccc ttataacgga atcactcttt gtacagggag
     5521 tattgcagaa gatccagaaa atgatgtata tgggatttta gcagaatttt gccaaagaga
     5581 tcgaatccca tttgcacatg ttcgaaatat taaattttta gctaatcgag atttctatga
     5641 ggcaccacat aaatccgaat acggttcatt agacatgtac cgcatactaa aaacaatgta
     5701 tgacaacgga tttaacggat acatccgtcc tgatcatgga agaatgatct ggaatgagac
     5761 aggaagacca ggatacggct tatatgatcg agcattaggt gctgtttatc taaatggatt
     5821 atgggaagca atcgaaaaag gaaacaaaag taaagataat tagtgaaaaa aactgtaatt
     5881 ttattatgta aataagaagg atgttcaaat tcttgataca taaatccgga ggttgttaat
     5941 aagcattcgg atttatgtat cttatttggt ctaggaagta acttgatttt cttttttcga
     6001 ttgatccatg agtgcttctt cttttaattc aaagatatat tctgagatcc tcataatcac
     6061 ttttttccag tcttctggtg tgatgattcc ttttaaaata tagatttttt tgatatgaga
     6121 aggcaattgt ttcaaagggc agtcgctgat aacgatgttg cagttctcca taaaatcatt
     6181 ttgtattttg atcgattcat ttgaaaaaat ttgttctatc ttaacgatca gatctttact
     6241 gatataaaaa tcacgcgtat agttcacacc aatcacaatt cgcggctgtt ttttgtagcg
     6301 atcatacaaa tgtactaggt cttcaaccat ccattgcaca ttttcctttt ctaaaataac
     6361 tggtaacaag tgttgatttt gttctctgaa atccatataa aattgttcaa tggcggtttg
     6421 gacttctttc aatacgctat gttcttgata aagcatgaaa gtaggaaggg gcgtatttgg
     6481 ttgattgaag ttaagaaatc cattataaag tttatttcgt gcgaatgcca atgagtaaag
     6541 aatcttttct ttttcaggta taaacaaatc aaatacagta caaagggact ccaccatttc
     6601 tcttgaataa agaacatgag gaacgtttgc tttttttaaa agtttgtatt gctccaagct
     6661 tgtttcttcc gattcgattt tcgtgataga aagtcgagct aaaatattca ctaagatgcg
     6721 ttgttcgcga caaattggca gattaggtcg caaaatatct acagagagat caataaaaaa
     6781 gtcaatatat tcatactctg tgaattcttt ttgtacgtct ttcacagaag ttgcaggaaa
     6841 attctcttta catattttta atagaaaata taatttatct atcataccgc tatttagtcg
     6901 gtcaagtact tcttttttaa aaaaagtatt catcataaaa gccactactt gcggatcttc
     6961 atcataaaat tctgtagcag tacttgaaaa gatgttccaa taaatatcta atgctgcata
     7021 ttgaatttgt acctcttttc cagaaaaaga ctttctgcca ttattagaaa aggagataga
     7081 caaaccgtac tatgctaaaa atgcattaat ctttttcatt ttcgtataca aataagaggc
     7141 actatagttt gtttgctgac tgaaactgat aatcgaattt gtctgacagc tgaacaaaga
     7201 agtcaagatc tgataactga ctgattcgtt gcagtatctt ccaaatagac gataatagca
     7261 gtaagccgga tcaattcctt caaaattcaa ctttaaaaaa ggtttttgct tatcaatatg
     7321 cagttctgtt agattgagac gctcaaagtc ttgttctaat tctttgatgt atagtaaaaa
     7381 atttgattca gacatatgga aatgatctgc taaatattgt gtagtaataa agaaacgatg
     7441 ttcagatagg aaaagaaaaa tatctaattt tgtattgctt tttttcttca agataaacgt
     7501 tttaatcgct tcttgagttt gcatgaaaga ggctcctttt tacttaaatt gttaaaactt
     7561 gaaaatatta ctgttttttt cataaattta gacaaaaaaa cagtaaacat ataggtagaa
     7621 tctctactat cttatttctt gcatacaaaa aaatcaagga gtgagagagt tatgagatat
     7681 tcaaccatct taatatcaac atttgcagcc ttaagcttat cgcttactat aaatcagaca
     7741 gtccacacag aggagacaga aacagccaaa tggacagaaa ccaagtgggc caacgtcaat
     7801 cgaattacct ttcaggatga attttcatcc ttcaattcag gagtcgcaaa attaagaaaa
     7861 ggacaaacag cgaccatcaa agcaaaaatt gactattcgg gagacttagc acctatacta
     7921 caatcagctg ttacatttga gaatgttgat cccactttgt cgattggcta tgacgagaac
     7981 actttgacta tccaacacaa gcaagcgata tttaccttca ccgttacatt aaatcaggac
     8041 ttgttaaaac cagcgctttt tactgtcaag gtatcagatg gactaccgaa cgatcatcat
     8101 ttaacgccgt atagtcagcg gcagacaatt gagcaagatt cagccatcga ctcaggagga
     8161 gatcttgttg aagaaccaac agataaacca gaaaatgaaa ataagccaga agtaccgcct
     8221 acagaaaatc cagacggaga acagaaaccg gaaatagaac ctggagaaga acccgataca
     8281 gaaactcaac cagagccaga caataaatca aaacctgaaa taacccctgg agaaaaacca
     8341 gatgtaactc cagaaccaga tacggattct ggaaaccaaa cagtaccgga aacaaatcca
     8401 gacacagata atgaaactga aaatccagaa aaaccagagg tagatccaga agaaaaacca
     8461 gatgtgactc cagaaccaga tacggacgca agagaccaag gaataccaga aaaaataaac
     8521 aaaaaaacaa ttcaagagga tggaaaaaaa gagagtaaaa aatcaaatct agcaattcta
     8581 aaaataaatg aagagcagct gaataaaaaa tcacgaatat ttgatagcgc ccaatcagct
     8641 gaaaccttaa aaagtagcaa ggatacaacc tttgctgtcc tgaaacgaaa aataaacagc
     8701 taccaaaatc cggagagtca caaaacaaag ttatactgtg gagcggaatc attctattaa
     8761 gtatagcaac aatgctttct gcaaaaagat ttaaacaaaa cagatctctc taacatttga
     8821 ataatgtctg tgaacgcatt tttcaaataa aaagaaatat ccagaaaatg accccaaaat
     8881 accagattga aatctgatat ttggggtcgt tcatacttta tctttcttca tatttatttt
     8941 aagggatatc tgtttctttt ggaagatggt tcgggatctt tttcactttc aatacatcta
     9001 aaaagaaggt aaagatcgga atgccgacaa tcaagcccca aacgccaaag aaccgttcgc
     9061 taacaagtaa gaccacaaat gtataaaaaa tcggcagttc tgttttgctg gacatcaact
     9121 tcggattcag cacataagac tcaattagat ggacaatcaa aatcgtaatc aggatataaa
     9181 caacatctcg gatgcctcct tgggaataag ctatgaaaga tagaggaata caagaaatga
     9241 taacacctgc tactgggatc agacttaaaa tgaaaatcat gatcgctaaa ctcagcagct
     9301 gatgaaagcc aaatacagcc aaggcgattg tagtgagcaa cgtatttaaa agagcgacaa
     9361 caaattgagc ttctaatacc aaaccaaatg tattgacaaa cttatcggca aaatagtaaa
     9421 tatcttgaaa gaaccaagaa aattcacttt ttaaaaacaa tcttgagaat acaattgttt
     9481 ttttcttttc aatcataaaa aagaaactta agatgaatga catagcaaac gccactgcca
     9541 tttttcctat atcctgtaaa tagccaagcg caatcgaagc gccattttga agctgttcaa
     9601 aaaaatcgtt tcgctcaata taagtatgga tatattgtaa aacaggatct gcatcttttg
     9661 gctgatgctc gtaaaaatta ataacggact ggatcatgtc aaatgtttga tgaataagca
     9721 tcgggatata ctttgtgaat gccagataga taaggtaaac gatcagtgta taaatcatga
     9781 tagctaaaac acctgtaggg attcggacaa atcgctgaat gatttgaaca agatgaatca
     9841 tcaaataagt aaagataaat gtgagcaaaa tggttgtaat catacttcta actaagaaaa
     9901 gaacaaaaac gacaaataat aaaacagtga gacggcgtat tttttcattc gccaaaaaac
     9961 gctgataaac ggtcaaatcg acaacctcca aataacaaaa aataaacacg ttattcatta
    10021 tatgatggaa tatgaaaata agaaagagca aaaagaaaaa ccgctaaatg acgttatctt
    10081 atgagttggt tttcctcata aaatcgttca tctagcagtt tcagcccttt ttctggtctt
    10141 gatgaaaatt atctagcatt tttcgggtca gatgttgcag tgtatccaat tcttcctgtt
    10201 caagtggaag ataagacttg atcttttgtg gaataccttc tgcttgtttt tgcaacgcag
    10261 caccttgagc agtcagcgta acatttacga ttctttcatc acccgtttca cgctgacgat
    10321 taatataatt tttttctgct aactttttta gtaagggagt cagcgtacca gaatccagac
    10381 acaaatcatt tcctagctct ttcactgaag tctttttctt ttcccataaa actaacatca
    10441 caagatactg ggtataggta agattcagcg gcttgaggag gggagtgtag tgtctaatga
    10501 tttctttaga ggcggcatat aaaggaaaac atacttgatt aactaaatat agatcttcca
    10561 tttttccacc tccgctgttt tactatagtg tatcatagcg gatttacaca aaaaagtcta
    10621 ttcactaact gctttcagag taacttctat atttccagct gtagcttttg aatacggaca
    10681 cactttatgc gctgcctcta ataattcttg cgtttcttcc agagaaagac cttcgataga
    10741 tccttcaatc tctacaccta agacaaagct cgtttcactt tctgaataaa gggaaacttg
    10801 tgcactgaca gtagaagccc cagagatatt tttttgctgt ttgatcaatt ctaatgcact
    10861 gttaaaacat gcactgaaac cagctgcaaa caattgttca gggttggttg cattttcgac
    10921 gcgtttacca ggagctgcaa tattcaattc gaatgtacga tcgggattat gcacttctcc
    10981 agagcgtccg cctgtgttga tcattttcgt agaatacatt tttttcataa agttaccacc
    11041 tttaattata ttttatacaa ttgaatttta cacaatataa ttgtggaaag caaagaaatg
    11101 gattggcata agataaataa gagaaagcac atgaaacaga cgcgctttct cgaatagcaa
    11161 acatatacaa aaaatatcag agacaaaaaa taatctcatc ataaacatta catatatttc
    11221 tcaaaaagtt gttttctttc ttcaaaaatt acttcacgat ggacctgatg aagcatccat
    11281 aaataaccaa aaggatctct gaacattgag ttagtcacac caaagtccat catttctgtc
    11341 atttcttgaa tctctacaca gccatttcgt aatgctttat tatgtgtgtc ctcgatatca
    11401 gagacgatga tattgaacca gatcgtattt ggatgttcag cagtaggagc agtcaaacca
    11461 agagacctgt tttcatccag catatggaat ctcgtaccat acaaggtaaa aatgacttca
    11521 ttattgcctc tagggtaatc tgttgcctca atcagttgta tttcatcaaa gattgtttga
    11581 tacagttcta gtgctttcaa actatcattt aatacaaaat cgatttccgt tcctttatgc
    11641 ataaagatct cctctccttt tcagtcgaac agttactaga ttcttttatt ataccagcta
    11701 aagaaaacgc attcaaaaaa atagcggatt tacttaccaa ctcattcgat tggatgataa
    11761 gtaaatccgc gtcccattac ttctgctacg ctgctgacaa tgacaaaggc acttggatcg
    11821 atttcttcta tcaatggttt cattcgggaa aattcatgat cagtcacaac gatcaataaa
    11881 acctcttttg actttcgatt atacccgcct tttgcctcta gcagggtaag gccacgtcca
    11941 atctcagcac ttaagcgaag gcggatgtct tccaaataat gttcactcat gaccataaca
    12001 gatttctttc gattgatacc tgtttcgata taagtcatga caattgaagt gattgcaata
    12061 gataaaatag cataaagaaa ctcttctatt ccaaaaataa acagattaag agaaacaacg
    12121 aatgcgtctg taaaaaataa gccaatcgaa gtattcaagt ggaaatattt tttaaaaatg
    12181 aggggaggga tcgtcgtacc gccgctagaa gagttgtttt tatataaaat cgcaactcct
    12241 aaagcaaaaa tcgcacttcc aaaaatgaca gacaacaacc gatctgaagt aatcattgtt
    12301 tctggaacta ttgcaatgat taaaggaaaa accaagcttc cgaaaagtac tttaaaaaag
    12361 ggttttttac ctaaaaatag aagagctagt accaacataa cgatattcaa cactaaaata
    12421 actatagctc ggtcaaagcc taaagcagct tccagtaata ttcctagacc gctgacacct
    12481 ccagcagcaa tatgatgagg ccctaaaaac atattgatag cgactcctaa tatgaagagg
    12541 gcaaaaataa tcttaatgta tgtccatata aattgtctcg tttctttcat ttattcactt
    12601 ccttaattca ttaataatac tcttatctta ttataactta cttttcacct taagttaact
    12661 ttaattaata aaaaaattag gctagtctaa attattatgt ttcattcttc tagaatatac
    12721 ttctacgcca aaaactgtta aaaaactttt actttagaaa attggagaaa ttgatcgctt
    12781 aagataaatt caggacacta aatagtacag gaagtagact aaaactttta aaagttttaa
    12841 gtctatctat ttggcaagtt taaaaaataa aaattcggta tcaaaaaact gttaccgata
    12901 acatttttga aagcgttgtc ttttgcttct ctatcactta tagtgatgac gtcatattaa
    12961 aacacaagga ggaaatcaat ggatacagct gcaatttatc atcgaccgga aagtgaatat
    13021 gcatttctat atacgaatac aagatttcgt attcggcttc gtactaggaa aaatgatatc
    13081 aaaaaagtat atgtgctatg tggagatcca tataccataa caactgaaaa atggtatcaa
    13141 aaacaacgac caatgaaaaa atgtctaagt accaatgttc atgattattg ggaaattgaa
    13201 gtgacgagcg aaacaagtcg cttacagtat gcgttccatg ttgttgggga agataacacg
    13261 gactgttttt acggtgatca aggaattttt ccttatcgag aaaatgtgat tactgaacca
    13321 aatttttatt ttcgaatacc ttattttcat caaatcgatc gattcactat tccagaatgg
    13381 gtaaaagaaa cagtatggta tcaaattttt cccgaaagat ttgcaaacgg ggacaaagca
    13441 aatgaccccg aacacacttt accttgggga agtaaagatc cagaccgaga agactttttc
    13501 gggggagatt tgcaaggtgt gatggatcat ttggattact tgacggacct tggcatcaat
    13561 ggcatctatt tttgtcccat atttaaagcc acatccaatc ataaatacga tacgattgat
    13621 tattatgagg tggatcccgc ttttggcgat aaaaaacttt taaaaaactt gattgatgaa
    13681 gcacataaaa gagggatacg tatcatgttc gatgctgtat tcaatcatat gggtgtccat
    13741 tcaccacagt ggcaagacgt tctcaaaaac ggagaaaaat cgatctataa agactggttc
    13801 cacatccgtt ttttcccagt agattcttat cagatgactg acttaccaga aactgctgaa
    13861 aatatcccat atgatacttt tgcttttact ccattcatgc cgaaactaaa tacagcaaat
    13921 ccagaagtgc aaaaatattt gctggatatc gcaacttatt ggataaaaga attcgatatt
    13981 gatggatggc gtcttgatgt tgctaatgaa gtcgatcatc atttttggaa aaaattcaga
    14041 gaagcagtta cagaaatcaa accagacatc tatatccttg gagagatttg gcattcctct
    14101 caagcttggc tgcaagggga tgaattccat gcagtgatga attatgcatt tacagattcg
    14161 attaaagatt attttgcaaa aaagaaaatc acagcttcac aaatggtcag tggaatgaat
    14221 catcagcaaa tgctttacag agatcaggtg aacgaaggaa cattcaactt attagattct
    14281 catgatactg ccaggatctt gaccctttgc caaggtaaca aagaattgat gaagtctgtg
    14341 atggccttta tgtttcttca aaaagggtct ccttgtatct attacgggac agaaataggg
    14401 atgactggtg aagatgaccc ggattgccgc aaatgtatga tatgggagaa ggaggaacaa
    14461 gatctagagc tattcggttt tataaaagaa cttgtttctc taaggaaaca gttgtctaag
    14521 attatttctg aaggttctac tcagtggcta atagtaaatg atcgtgaaga taagctctat
    14581 ttcacacgga aactagaggg acagatcatc tatgtttact ttaatcagtc aaaagaacct
    14641 tgggtagtag aacaggaaaa tgaggtgatt cttagtcaaa attgtcagtt gttagaagac
    14701 gggaaagcag agatacgaca atacggattt ttgatcatgt gttgaaaact gttaccggta
    14761 acaacaagaa agcgtttgca aatgaagatt tgtgcccatt ataataaggg tgtaacagca
    14821 cgttgaatac aaaggaggaa atgaagatga aaaaaagaag tgtgaaacag ttgggtttag
    14881 ggttaatctc tttaggaatc ttaggcggat tagcggcttg tggcaatggt aactcttcag
    14941 cagaagggga agccgatgaa ggaaaaacgt taacagtttc cgttgattct ggatataaag
    15001 attatataaa cgaaataaaa aacactttcg aaaaagaaaa cgatgtgaaa atcaagctag
    15061 tagaaaagga catgttcgat cagttggaat cattagcgtt agatgggcct gctggaaagg
    15121 gacctgacgt tatgatggca gcatatgacc ggatcggtgc gttaggacag caaggacatc
    15181 tggcggaagt aaaactggga aatgaagcag cttacgatga aacagacaaa gcccaagtga
    15241 cttatgatgg aaaaatctac ggagaacctg cagtaatcga gacccttgtt ttgtactata
    15301 acaaagattt agtcgataca gcaccatcaa cgtttaaaga tttggagaat ctatccaaag
    15361 acagtcgttt tgcttttgag tcagaagcag gaaaaaatac aggattttta gctaaatgga
    15421 ctgactttta ttattcctat ggtttaattg caggttacgg tggctatgta tttggtgatg
    15481 atggcacaaa tccttcagat atcggtttga ataatgcagg tgctgtagaa ggaatctctt
    15541 atgcaacaga ttggttcaaa aatgtttggc caaagggaat gcaagacata aaaagtgcag
    15601 gagacttcgc cagccagcaa ttcatgtcca ataaaactgc agcaatcatt gacggtccat
    15661 ggcaagcgca aacatacaaa gaaaacaata tcaattatgg tgtagccaaa attcctactc
    15721 taaataatgg acagccttat caaccgtttg gcggaggaaa aggatgggta gtcagcaatt
    15781 atgctaaaaa caaagacctc tctcaaaaat ggcttgatta tgtaacgaat caggaaaatc
    15841 aagaaaaatt ctttgaaatg gtaaatgaaa tccctgcaaa ccaacaagct agggaaacag
    15901 caaaggctaa aaacgatgaa ttaacaacag ctgtgattga acaatatgaa acagctcaag
    15961 cgatgccaaa catcccagaa atgggcgaag tttggacagg tgcagaaaac ttgatgttcg
    16021 atgcagcatc aggttcaaaa acaccaaaag aatccgcaga tgaagcagta aaaacaatca
    16081 gtgaagccat agaacaaaaa tactgattaa aatgataacc gtccattttt ataaaaaatg
    16141 gaacaggtgc ttcagataaa agtggcaaga gcgagatgga aaattatctt gctcttgttg
    16201 ccattatctt aaaagcagtt tgcggtttag caagtttaga gggggcattc agatgcagca
    16261 agataaaaca agcaatatca acgaaaaaaa aatcacgctt tgttcactta ttccagggat
    16321 ggggcagttt gtaaatggcc aagcttttaa aggtatcgtt tttttcgcca tattcgtcct
    16381 attcattggt cagatgcttc tgggcggatt tcatgcattg gtaggattgg ttactttggg
    16441 aagtatacca atggaagatc attcattatt tattttgatt gaaggcactt tgcaattcat
    16501 cattacaatc attttcttga ttttttacgg tttgaatctt ttagacgctt atcgagtagc
    16561 gaagatgcgt caagagggca aaaaaatcaa tcggacgatg aaggaagtca tacataatat
    16621 aggtgatgaa ggttttcctt atctattgac acttcccgct tatcttctca tgatttttac
    16681 gatcattttc ccagtcctag ttacattgtt cactgcattt actaattatg acttcaatca
    16741 tattccacca gccagtttaa ttgattgggt aggggctaaa aactttttta gtatcttctt
    16801 tttaagctca tatcgtaata ctttttttgc agtcttttcg tggaccgtga tttggacttt
    16861 atgtgcaaca actttgcaaa tcacccttgg tattctcaca gcggttgttg ctcaccagaa
    16921 atttattaaa ttcaaacgta ttttcggtat cattttctta ttaccatggg cggtcccagc
    16981 ttttattact atcatgagtt tttcgaatat attcaacgat agcgtaggtg ccatcaatac
    17041 tcaagtgatt cctttcataa accagcttcc ttttgtagat atcagtgcaa tctcttggaa
    17101 aacggacccg aactggacga aagtagcaat tatcttagtg cagggctggt taggatttcc
    17161 ctatatctat gtgatggtta caggaatctt acaatcgatt tcagaagact tatatgaagc
    17221 agcaaaaatc gacggagcaa atgcatggca gcgcttttca aacatcacgc ttcctgctgt
    17281 cttcctagtg gcagcgccaa catttattac acagtatacg gggaatttca ataatttctc
    17341 catgatctat ctgtttaaca atggcggtcc cggaagcgta ggcggaggag ctggttctac
    17401 tgatattctt atttcctgga tctataaact tacgactgga acttctccgc agtattctat
    17461 ggcttcagca atcacgttga ttatctccgt catcgttatc tgtgtgtcat tgctggtatt
    17521 taaaaagaca aatgcgttca atatggggga ttaacaatga aaaaatttca ttcgagtgct
    17581 cgattccaaa aacgactttc tcactttttt acttactttt ttcttttaat actgtcagtc
    17641 attatcatct atccgctttt gatcacagca agtactgcct tcaagtcagg aaatattgca
    17701 gcttttagtt tagatctttc aagtgactgg actttaggaa actttactcg gctattcagt
    17761 gaaacacttt acgggacttg gtataaaaat acattgatca tttctgtcct aacaatgatc
    17821 gtacaagtga ctgtagtaac tttggcgggc tatacttata gtcgctatcg ttttatcggc
    17881 cgcaaatcaa gtctgatgtt ctttttagtg attcagatgg taccgactat ggcagcattg
    17941 actgcctttt atgtcatggc tttcttatta gaagcgctcg ataagtactg gttcttgaca
    18001 atgatctata taggcggtgg aattccaatg aatacttggc tgatgaaagg atactttgac
    18061 actgtaccaa tagatttaga tgaatctgct aaacttgatg gggcagggca tttcaggatt
    18121 tttttccaga ttgtattacc gcttgtcaaa ccgatgctcg ccgttcaagc attatgggct
    18181 tttatgggac cttttgggga tttcatgtta gctaagttcc tgcttcgaac acctgaaaat
    18241 cagactgttg ctgtcggctt acaaacattt atttctgatg tgacaaacca aagagtctct
    18301 ctttttgctg ctggggcaat tttgattgca ctaccgattt gtctattatt cttcttttta
    18361 caaaagaact ttgttaccgg cctgacatct ggcggaacaa aaggatagga gcagagtatc
    18421 atgcaaacat ttccgttaaa ctatttttcc gcattacgta cgccaactca agtgtttgcg
    18481 ggacgcaaac tattatcatg gcctaaattt ttcttgatat ttgtattttt agtttccttg
    18541 atggtcatgc ctgtgactct tttttatgcc aatcagatcc aagcgattcc attagaacaa
    18601 tttctatccg ttcattcgct gatcgatgaa caaggaaccc aaaaattttc tgaacttgaa
    18661 ttatctgaaa ctgggttaca agcatcgcaa caaacgatag ctgtgacccc tgaaattttg
    18721 gtaggtgtgt ctttatctga gaaacaacaa tccgatcatg gaacttttat tgattttgag
    18781 aaggaacaat gggtgatcca acaaaaggat aaatctggta tacgccgcta tacgatgaat
    18841 tatagcccat ctttccaacc agattctgta cgtacgcccg aagatttcca acgattttta
    18901 gaaagagaat tttatgccag taatcgtcca acaatcattt tatcttatag tttaagtctt
    18961 ggtcttgtcc tatttgtcat gacgagtctc attttattcg gggcttcctt tttcttgtgg
    19021 atgacaagaa aaagccaact ttcatccatt catacattca aagagagtgc aaatttgatg
    19081 ctgaatgtta tgggaatagg ttccatggca gcagcagttg tagggtttat ccactttgac
    19141 tttatcttga tgctaagtgt ccaaacaacg ataactgtct tgctgctttt atgggttttt
    19201 gtcaaaacaa gattcaaaga taagcgtgtc gaatgacaag tttaagcgat ttatgcgaca
    19261 atgaatgtga aaaaaatgaa aaataaagta gtttttgaaa aatttctgaa aaaattaaac
    19321 ttcttagttg attcttttct tttttagtat taacgtaata gggtagtttt tttaaggagg
    19381 aaaatcatgt tagagaaaac tttatacaat caattatttt cacgttcttt ctcgctccct
    19441 gtcgaagtaa attattggga cggtacaacg aaacaatacg gagatacgga cactcctccc
    19501 caaatcaaaa tcaatatcca tgaagaaatc ccaatgaaag agattacaag taacgcatct
    19561 ttggcactag gagaagccta tatggatcat cgaatcgaga tcgagggaag tatccaagca
    19621 ttgatcaacg atgcttatca gcaagcaaac agctttttac gaggaaaaga ttatctaaaa
    19681 tggctgccaa agaaagaaaa acatacgaaa caaaaaaaca aagaagatat ccatgcgcat
    19741 tatgacttag gtaacgactt ttataaactt tggctagatc caactttgac ttattcttgt
    19801 gcttatttca atacacctga agatacgttg gaacaagcac aaatcaataa agtccatcat
    19861 atcttagaca aactctttat caaagaaggc gaaacgttgc ttgatatcgg ttgtggctgg
    19921 ggaacattga tgttcactgc taccaaagag tataatgtca aggcaactgg gatcacacta
    19981 agtgaagaac aatacgattt gattaccaaa agaataaaag aagaacattt agaagataaa
    20041 tgccgtgtat tattaatgga ttaccgcgaa ctaaaaggcg aaacgtttga tcatatcaca
    20101 agtgtaggta tgttcgaaca tgtaggttct gaaaatcttg aaggttattt caaagtggta
    20161 aaagatttat tgaaaccaaa aggaacagct ttgatccatg gaatcagccg acaacaaggc
    20221 ggtgcgacaa atgcttggat caataaatat attttccctg gcggttatgt ccctggtgtt
    20281 actgaattag ttgataatat tacaaaaaac gatttgcaac tgattgattt ggaaagctta
    20341 cgtcgtgact accaattgac attagaacat tggacaaaga attttcatca agtccaagat
    20401 caagtcacga aagaaatggg tgattccttc tatcgaatgt gggatctata tttacaagct
    20461 tgtgcagctt catttgaatc aagtaatatc gatgtgattc aatatttgct tgttcaacct
    20521 ggaaacaatg acatcccaat gcatcgtgca taattgaggc aaaagtactg tttaaagact
    20581 atttaaaaag aagctaagtt ttatgatgct ccagaagtga acttttttgt ctaacttcta
    20641 ggattgtctc taaagcttag tttctttttc tttatatttt cataataaaa gtttggttct
    20701 ttaattttta aacatgacag aaaatttttt cttcgtt
//
LOCUS       DAZBSA010000048        20468 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000048 DAZBSA010000000
VERSION     DAZBSA010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.048"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(541..1566)
                     /locus_tag="ACHGMT_001594"
     CDS             complement(541..1566)
                     /locus_tag="ACHGMT_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN6835337.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            1841..3526
                     /locus_tag="ACHGMT_001595"
     CDS             1841..3526
                     /locus_tag="ACHGMT_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFN6835338.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            3540..4823
                     /locus_tag="ACHGMT_001596"
     CDS             3540..4823
                     /locus_tag="ACHGMT_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN6835339.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            4893..5762
                     /locus_tag="ACHGMT_001597"
     CDS             4893..5762
                     /locus_tag="ACHGMT_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6835340.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            5759..6595
                     /locus_tag="ACHGMT_001598"
     CDS             5759..6595
                     /locus_tag="ACHGMT_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6835341.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            6621..7688
                     /locus_tag="ACHGMT_001599"
     CDS             6621..7688
                     /locus_tag="ACHGMT_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFN6835342.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            7685..8512
                     /locus_tag="ACHGMT_001600"
     CDS             7685..8512
                     /locus_tag="ACHGMT_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFN6835343.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            8557..9582
                     /locus_tag="ACHGMT_001601"
     CDS             8557..9582
                     /locus_tag="ACHGMT_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN6835344.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            9579..11762
                     /locus_tag="ACHGMT_001602"
     CDS             9579..11762
                     /locus_tag="ACHGMT_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFN6835345.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            11762..12892
                     /locus_tag="ACHGMT_001603"
     CDS             11762..12892
                     /locus_tag="ACHGMT_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFN6835346.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            12889..13566
                     /gene="pgmB"
                     /locus_tag="ACHGMT_001604"
     CDS             12889..13566
                     /gene="pgmB"
                     /locus_tag="ACHGMT_001604"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFN6835347.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            13650..14177
                     /gene="msrA"
                     /locus_tag="ACHGMT_001605"
     CDS             13650..14177
                     /gene="msrA"
                     /locus_tag="ACHGMT_001605"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFN6835348.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14378..15064)
                     /locus_tag="ACHGMT_001606"
     CDS             complement(14378..15064)
                     /locus_tag="ACHGMT_001606"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFN6835349.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            15144..16682
                     /locus_tag="ACHGMT_001607"
     CDS             15144..16682
                     /locus_tag="ACHGMT_001607"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFN6835350.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            16802..17269
                     /locus_tag="ACHGMT_001608"
     CDS             16802..17269
                     /locus_tag="ACHGMT_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFN6835351.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            17803..18231
                     /gene="rplM"
                     /locus_tag="ACHGMT_001609"
     CDS             17803..18231
                     /gene="rplM"
                     /locus_tag="ACHGMT_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFN6835352.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            18247..18639
                     /gene="rpsI"
                     /locus_tag="ACHGMT_001610"
     CDS             18247..18639
                     /gene="rpsI"
                     /locus_tag="ACHGMT_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFN6835353.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(18727..19953)
                     /locus_tag="ACHGMT_001611"
     CDS             complement(18727..19953)
                     /locus_tag="ACHGMT_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6835354.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(20025..20327)
                     /locus_tag="ACHGMT_001612"
     CDS             complement(20025..20327)
                     /locus_tag="ACHGMT_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6835355.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNTFKIT"
BASE COUNT         6605 a         3683 c         4281 g         5899 t
ORIGIN      
        1 gtctaaaata atttgtctaa ctagaagaaa agtgggaaag gatcttaaca acatcggttc
       61 gtatccaatt aaggtatcgg aacgatcatc ttccaagaga agtcaatcaa caaaagtaaa
      121 gatagatcat tttttgaatg caaaataaat tgatgggaag atagagcggt tatttttaat
      181 attttgctag ctattattga ataattcctc gaataaaaca aaaccagtag acatgtctgt
      241 tattctattt atgttttaga gataagtatc cccaaataac atggattgaa ataagggtaa
      301 atgtgtttaa aaaaacgaac tataaaaact tatacacaac gtggttaaaa atagtttttt
      361 cttccctgtg gaaaaaatag tttaacacaa acattatagt tatccacagc tatctaaaac
      421 agatggaaaa taagaaaaat tcgctaaaat accgggaggg ggtaacgagg gatgagtgat
      481 tgaaaaaaga acagagtaac tgacttatcc tcagtgtttc tgttctttta taattctgtt
      541 ctacatttct tgttgttgga tcgaatctgt gagagtaatc tcggttgaaa gaatgattct
      601 tttttcttca gggtattggt ggtggattag ttggaataaa agttctgtgg ctttgattcc
      661 taactgattt gtcggaatgt ctatccgagt caactgtggc gaaaataggg aggacaataa
      721 gggatcactg aatcctgcga tcgaaagatc atcaggtatc tgctttccag attgttctgc
      781 cactttgtaa atactcaatg attttaatgc atcggtagaa aaaatcgcag ttggtctttc
      841 tcgtagcttc attaaatcat tcattgctgc catgctatct tctaagcgaa agccgccgtc
      901 tataatccat tcattacgga tatctaggtg gtgatcaaat aagcattgac ggtaaccatt
      961 gagacgatca ttggatacat aaacttctgt cggagcatgg agacagccga tatgacggtg
     1021 tccattcttg atcagtgttt ccaccaaatc gtaactatcc tggaaattat ccgtgtccac
     1081 tgagtagatg ttcgtgtagt cgtttccgac ttttccgatt accacaatag gaatattgta
     1141 ttgatccaat tcaaaaagaa gtgtttcatc catcgtagag cttaaaataa taattcctcg
     1201 gatcattttt tcttggatct ttttcgcgca tttttctagc tcttcttctg gtgtttttgc
     1261 cgtttgtaga atcaagtcga agttattttg ttcagaaacg gcagaaatgg attgaataac
     1321 atctgaaaaa aaaggattgc cataggtcgt atgagaagta cgatgagata atgctagaat
     1381 cgcgtcaaaa ccatttcctg acagtcctct tgcaattttg ctcggttcat aattcaattc
     1441 tttgattgct tgaagtacac ttgcttttgc tttatcagag atatttggct ggttatttaa
     1501 tactctggag acagttgatt ttgagacatt agcttttttc gcaatgtcat aaatagtagg
     1561 tttcaatttt atatgcacct cctttttggg agcgttttag aataattcca tagtacaata
     1621 gacattcttt tttgtctatg attttgaaaa tttaggaata gcttgcatga tcatgagaga
     1681 ccatattttt ttaagaagct attaattatt gatacataag ctttcttatt tgtatttttg
     1741 tgtatatcaa aattatttaa aaaaatgcta ttgacagtaa gcgttttcca taatatgatc
     1801 aaatcgttgg gaacgctccc aaaaaagaaa ggggaatccg atgtatacaa aacttttaca
     1861 agcgatttat cgcgactcag atttgtctga gaaaaccgcc tctatgattg aacaaaaagt
     1921 cagtgtttat cagcagaaga aaggccaggc aggcaaactg cattgggacg aagaagatat
     1981 tttcttgatt acttatgggg atcaattttt tgaagagcag caaccaactt tgcgtacatt
     2041 taagaaattt tatcaaaagt atttggcaga tacatttaga gttgtacatt tcttgccatt
     2101 tttcccatat acatctgatg atggtttctc cgttgtaaat tatcaggcaa tcaacgaccg
     2161 tttgggcaca tgggaagata tagaagaaat gagaagcgaa acacggttaa tgttcgactt
     2221 tgtctgcaat catatgtctg ccgaaagtac ttggtttcag gaatatttaa acttgaatga
     2281 tacgtacaaa gattttttta ttgatttgcc actggataca gatacaagca gcgtgactag
     2341 accaaggacc agcccgttac ttacaacgtt cattgacaaa aacgggaaag aaaaacacgt
     2401 atggacgact tttagcgaag atcaagtaga cttgaatttt aaaaatccag aagttttatt
     2461 gaaaatgatc gatattcttt tgttttactt agaaaaaggc gcttcattta ttcgcttgga
     2521 cgctgtggga tttttatgga aagaactagg aacttcttgt atacacctgc cagaaacgca
     2581 taaaatcatc caattattcc gtgcaatcgc tgatgaagca gctccaggaa cggtattgat
     2641 cactgagaca aatgtgccac acaaagataa tatcagttat tttgggaatg gcgagaatga
     2701 agcacagatg gtctatcagt tttcgctacc acctttgacc cttcaagcga ttcgttcggg
     2761 taatacaagt aagttgaaag aatggttaaa aaccatcgat ttcgcatcga aaaaaacaac
     2821 tttttttaat tttttagcct cccatgacgg tatcggattg aatccggtac gaggtattct
     2881 gccggaaaaa acgatccttg aattagtgga acagctgaag aaagagggta cccgagtaaa
     2941 ctacaagaaa aacccagatg gaacgaccag tccatatgag ataaacgcta cgtatctaga
     3001 tgcgttaagt tctccaacag atacgacgga atggagaagc aaacgctttt tagctgctca
     3061 ttcatttttg gtaacgttac caggcgtgcc ggcaatttat attcaaagtg tgctgggaag
     3121 tcgaaatgat tacaaaggcg tagaaaaaac aggagagaat cgaaaaatca atcggcaaaa
     3181 atttttgtta gcagatattg agaaagagtt gcagacagat ccattaagaa cagcagtata
     3241 tggtgaattg acccagatgc tatctattcg aaaaaaagaa aaagccttcc atccgaatag
     3301 tttgatttac ttactggact cagcagattc ttttatcagt tttattcgcg aagcgaagga
     3361 agagaaagtc ttagttatcc ataatttttc caatcaatca gcaggttatc ttttaccaaa
     3421 aggagattac ctagacctgt tttctggaca aattatccat tgcagtcaag aacgaaagtt
     3481 gatattccag ccgtatgaat atatgtggct gaaaataata aaataagagg tggaaaaaaa
     3541 tgaaaaaaat tattgttact acctgtctgc tagctactgg atttcttctt actgcttgtg
     3601 gtgaaagtca aagctctaac gaatcacaac agacaacgat tcaattcatg cactcttctg
     3661 tcgaacagga acgattaagt gtgatcaatg aactggttgc tgattttgaa aaagaaaatc
     3721 cagacatcaa aatcaagcaa gtgccggtag aagaatcagc gttcaataca aaaatcgtta
     3781 cgttagctcg ttccggtaaa ttaccagaag taatggaagt cagtcaggat tttgctaaag
     3841 tgatggataa agatgaactg attgatcaga cggcagtcca aaacgttatc gaggaagctg
     3901 gagaagataa ttattatgac ggagccaaaa accttgttcg ttcagaagat ggaaaaagct
     3961 atattgctgc accaattagc ggctgggttc aaggtatttg gtatgacaaa gaaaaattat
     4021 cagaagctgg tttttcagaa ccagaaaatt gggatgatat tttaaaaatt gctcaacatt
     4081 tcacagataa agagaataaa caatatggaa ttgctatgcc tactgctgaa ggaacaatga
     4141 gtgagcaagc gttctctcaa tttgctttgt caaatggagc aaatgttttg gatgatgaag
     4201 gcgaggtaac gatcgatacg gaaaagatga gagaagcatt gtcgttttat caaaacttat
     4261 cccaatacac gatgccaggc tctaatgatg tgacagaaat caaagatgct ttcatgaatg
     4321 gaacagcacc aatggcaatc tattcaactt acattttgcc ttctgtttat gaagcgggga
     4381 actcagaaaa tatcggtttt gctatcccaa cacaaaaaga tcaagctgtc tatggcacag
     4441 tatctggatt gacgatttct tctggtctag acgagaaaca aaaagaggct gctgaagcat
     4501 tcacagcttt cttatctcaa ccgaaaaaca tggaaaagtg ggtcttgatg tctcctggtg
     4561 gtgctcaacc agtgaataaa caagtagtcg aactggatgg ttacaaagaa aatgaagtca
     4621 tcaagtcttt tggcgagctg ccatctgaga ttgctagtgc ttttgatgaa gtacaagtct
     4681 ttggactagt cggggaaaag aatttcacga aaatgggaga tatcacaagc tcaggtgtga
     4741 tcggaaaagc agtgaaccaa gtaactgtcg gaaacgaaga tgtcgatcaa gctttagctg
     4801 acgcacaaaa aaatacgaaa taacaagaag ttctgccaat ggtgttcgaa aaaagtagat
     4861 agctattggc agattatcag ggggaggttt caatgaatcg tccaaggaaa cattctgact
     4921 taaaattagc catgggactt ttagcaccaa gcatgctttt attagctgtt ttagtgctga
     4981 ttccaatgct ttccaatatt cagatcagtt ttttcgatat gccgctgaat cctaactcag
     5041 acgccgtttt tatcggatta agcaattatc ttgaaatttt aagtgatagt gaattttatc
     5101 gttctttagg tttaacagtt gcttacacat tcttagttgt agcaggcagt acagctttag
     5161 gattagccgt agctattttg cttaaccgca agttcagatt taggaaaacg gtgcgttcac
     5221 tgattttatt aagttatgtt acgccatcta tttctttgat ttttgcttgg aaatatatgt
     5281 tcaataatag ttatggaatc gtcaatgatc tgttagtaga taggttgcag cttttcaacg
     5341 aagcaccgct gtggttcgac cagccagttg ctagcttctt tatggtcgtt ttgtttgcta
     5401 tctggagata ctatccgtat gcgtttattt catttttggc tattttgcaa acagtcgaca
     5461 aaacgcttta tgaagcagct gaaatggacg gtgcaaatgc ttggcagaag ttcaagatcg
     5521 tgacactgcc ggcaatcaaa ccagtactag caactgtgat tgttttacga tctatctgga
     5581 tgttctatat gtttacagat gtctatctac tgacgaataa agtcaatatc ttaggcgttt
     5641 atttgtatca gacagccttt gcgttcaatg atcttggaaa agcagcagct atatcgattc
     5701 tattgttcat cattattgca gcggtcgtct tgctctcacg aaagaaggtg gacttgaaat
     5761 gaaaaataaa aagagcagga aaatcttatt ttacgcgacg attttgttgc tattagctgt
     5821 caatttgttt ccatttttga tcatgctgat gacatcattc aaggattcac aagaagcaat
     5881 tgcaacgaca caaactattt ttccgaaaga atggactttt gaacattatc aggatatttt
     5941 caatgctgag atttttccat tcatcatgta tttcaaaaat agtttagtga tctccttgat
     6001 agcctccgta actgcagtag tgattgggat cagcggggca tacgctttgt caaaactacg
     6061 cttcaaagct cgctcgaaaa tcaatgctag cttttacacc gtttatatgt tttccggtat
     6121 tttgatcgtt gttccattat ttaaaatcat ttccggtcta ggtttgtatg ataccaatac
     6181 agcactgatt atcacgatgg tcgttcaaac acttcctact gcgatattca tgctaaaaag
     6241 ttattttgac acgattccga ctgatattga agaagcagca atgatcgatg gtcttagccg
     6301 gatccagatc attattcgga tcattatacc attggctatt tctggaatca tttctgtctt
     6361 cgtttattgc tttatggttt cgtggaatga ttatttattt gcttcgattt tcctgtcgac
     6421 ctccgaaaaa tttactttac caatcggttt gaacacatta ttcagtacac cagactacat
     6481 ttggggacga atgatggccg cttcattggt gaccgcttta ccagtcatca ttatgtatgc
     6541 gttatctgaa cgatttatta aaggtgattt agtcgaaggc ggcgtaaaag gataaaaaga
     6601 aaagaaagag gaaagaaaag atgaaaaaat tagttgcatt agaaccaaga atagccggat
     6661 tcaaagatta cgaagaccgt gagatcgaaa agaacgaagt acgtatccga gtaattttcg
     6721 ctactccaaa accaggaaca gaaattgtcg actttagagg attaagtcca tttatcgatg
     6781 aagaattttc agaagaatgg cgcctattct tgccaagaga agaaggaagc caacgcggtg
     6841 tggtatttgg cgaatttcaa ttaggaaata tgattgtcgg tacgatcgaa gaaacgggca
     6901 gtttggtgac agagtatcaa aaaggagagt tagtctgcac ttatggaccc attcgtgaaa
     6961 cgctgattac aaacggtgtt gataattata aactacgtaa acttgatcgg ttggatcaat
     7021 ggaaaaatgc agtatgttac gatcctgctc aattcgcttt aggtggtatc cgggaagcac
     7081 atgttcgagc aggtgatgct gtagcaatct tcggtttagg cgctatcggg caaattgcga
     7141 ttcaattagc taaaaaagca ggtgctacta ccatcgttgg gatcgatccc atagcgcagc
     7201 gtagagaatt agcagaaaaa tacggtgccg attacacgct agatcctatg aaagcagatg
     7261 tcggttttcg tctaaaggaa ttgacagata aacgtggagt agattcagtt attgaaacga
     7321 gtggcagtgc tgccgcatta caagcttctt taagagggat cggctatggc gggatcattt
     7381 cttatgttgc ctttgctaaa ccttttccag ctgggtttaa tcttggtcgc gaagcacatt
     7441 tcaataatgc aaaaatcgtc tttgctcgag catctagtga accaaaccca gattacccaa
     7501 gatggaaccg taaacgaatc gaggacacat gttgggaatt attgatgaac ggttacttgg
     7561 attgtgaaga aatcattgat ccagttgtgc catttgcaga aagcgcagaa gcttacatgc
     7621 gttatgtaga ccagcaccca gaagaaagta tcaaaatggg cgttcaagtc cgaggaggag
     7681 aacaatgaaa ttcggtacgc aagatcaagc atttttccca agagacattt tagaaaaatt
     7741 tagatttatc aaagactgtg gatttgaaga atacgaaatc gacggtaagt tgttaataga
     7801 aaatatcgac acagtcaaac aggcaatcaa agaaacaggg atcccagtag tgacagcttg
     7861 taacggctac gatgggtgga tcggtgactt tatcgaagaa cggcgtctta atggtgtacg
     7921 tcaaattgct gagattttgc gtgcactaag cgaggttgga ggcaaaggaa tcgttatccc
     7981 agcagcttgg ggaatgttca cttatcgatt accgccgatg gtttcacccc gaagcaaaga
     8041 aggagactat aaagcagtca gcgaatcact tcgttattta gataaagtag ccgaagaaac
     8101 aggtacggtt atttatttgg agcctttgaa ccgataccaa gaccatatga tcaatttgtt
     8161 agcggatgcg cgtcattaca ttgactctaa tcaattgaaa catgtgcaga tcatcgctga
     8221 tttttatcat atgaatattg aagaagatga tatttcaaaa gcattgacta cgcaaaaaga
     8281 gtatgtgggt catatccatt tagcagataa tcatcgctac caaccaggat ctggcgcaat
     8341 tgattttact cgccattttc aaacgctact tcaaaatgag tatgaaggtt atatggtata
     8401 tgaatgtcgt ttgcgaggag aaaatgaagt gaaaatttat caggaatcat tgcaatactt
     8461 gaaaaactgt ctggaaacag cgcgtcattt aaatgattta gagaaagaat agagaaagaa
     8521 taacaataag taaataagca aaggagggag aaataaatga agaaagtagc aatcataggc
     8581 gctggacaag tagcagaaaa agtacatgct gcttattatc aaaaaagatc agatgtctta
     8641 gaattggccg ctgtggttga tccagtcagc cagcgagcag aagacttttg ccgacgtaca
     8701 ggattttctc atgcgtatac atcagttaca caaatgctga atgagatttc tcccgatatc
     8761 gtgagtgtct gtacacccaa tcgctttcac ttcgaacatg tattggctgc cctcgaagct
     8821 aatgcttcag tgatgtgcga gaaaccacca gctatgacag ctgatcaagc aagaaagatg
     8881 gcagagacag ccgataaaaa cgggcagatt ttggcttatg gattccagca tcgtttttct
     8941 gacgaagcaa caatgatcaa gaatcatctt gatgttctcg gggatattta ttacgtagaa
     9001 gcgaatgcgc ttcgtcggtc tggtgttcca ggctggggaa cgtttatcga caaagaactt
     9061 caaggaggcg ggcctttgat cgattatggc attcacatgc ttgatacagc gatgtatatt
     9121 actggatttc ctaaagtaaa aagcgttcgg gcgtacagct tccaaaaaat tggtacgaaa
     9181 aaaaattcag ggaaattcgg gaaatgggat ccaagcaaat tcacagtaga agatgcaatt
     9241 tttggcacac tggaattaga aaacggcaag atcatccaac tgaatacttc gtttgctttg
     9301 aatatcagag aagacaaact gaatgtggct ttttgtggcg atcaggcagg tgctaatctg
     9361 tatccaggcg aactattcga agatgtagat ggaaagctgg tttccttaca aaagaccacg
     9421 ccagttaatg acccacatga actaagtatg gctcattttg tgaatcgggc gctcggtgac
     9481 cagactcaga tgattgcaga tggctggcaa ggatatgtca tccagaatct ggtggaaaat
     9541 ttatatcgtt cggcagaaac aggagaagtt attaccttat gaaagtagaa gaaaaacagt
     9601 ttgatttaga gtatatcaat aagtatgcga gtcagatgac cattggaaat ggattttttg
     9661 gtatcagagg aagccaggag gaagactatc ggaatcaagt aaggggaatg tttgcagctg
     9721 gtgtatataa ccgaccttta ggagcagaat cagccgagtt agtgaatctc ccagatgtca
     9781 tcagatggga aataaaaatc gacggtgaac atttttctct tgagacgatg aaagttcttg
     9841 catacgagcg ggcactagat ttagaaatgg gagagcttaa acgctatgcg ctcattgaaa
     9901 ctgctcaagg aaaaaaagtg gaagtcatta cttgtcgtgt tgctgatcaa aataatctcc
     9961 atcaggcagc cctgaaagtg gtcatcaagc cgttgaccga agactgccaa gtagatgtga
    10021 aaacgggtat cgatggacag caaaccaatt ttggtacaca gcagttggtt gaaaaagaac
    10081 tgcgagtatt tggtgacgat ttgatcagtg catcctacca gacaacagaa agtaaaattg
    10141 atattgggat tgcagctaaa ttcaataaag caggtgtatt ccaagcaaaa aatcgccgga
    10201 tcaccgcttc cttttcagaa aagatcagaa aaaatacgtc ctttacgctg gagaagatca
    10261 tcgtgctctc tacctctctt cagcaaacag acccgtttaa gaaaagtatt gcccaagccc
    10321 agaaaagcgt tagttataca gaaattttag agcagtcgat gtcttattgg acagattttt
    10381 ggcatcagca tcggataacg atccaaggcg attcttcttt cgatcagtta gcgatggact
    10441 tcgctgctta ccatttacag attatgactc cccgcaatga tccaagtgtt agtgtagggg
    10501 cgaagggact aactggcgaa ggttataaag ggcatgtttt ctgggatacg gaaattttta
    10561 tcctgccttt ctttttatat cagtttcctg aagtagcaaa gaatttgctg ctgtaccgtt
    10621 atgatcgatt agaaggcgca cggcaaaaag caagagatta tcactatcaa ggtgcacttt
    10681 tcccatggga aagcgcacac actggtcagg aagagacacc caaatttgct gcattgaata
    10741 tcaaaaccgg aacaagacaa gaagttgctt cagctgccgc agaacatcat atcagtgcag
    10801 accttgctta tgccgttcaa gagtatgtcc aagcaacaga ggatcaatcg tttttagaaa
    10861 caaagggaaa aacgctcatt catgaaacag cagctttttg gctgagcaga gctgtaaaag
    10921 tcgatgatca tttagagata cacagagtca ttggtccaga tgaatacaca gaatatatcg
    10981 acaataatgc atatacgaac tatctggcgc attataatgt ggccttagct gttaaatatc
    11041 aagctggcga tccgcttttt ttgaaagaat gtcaaaattt tttagataag ttatatttgc
    11101 cagtgcctaa caaagaacag ctgattccgc aagatgacac gtatctcagt aagccagtta
    11161 ttgatttgac cacttacaaa gaacgtcctg gcagccagtc tattttactt gattatgccc
    11221 gtagcgaagt aaatgaactc caagtcagca agcaagcaga tcttgtcatg ctgttatact
    11281 tattacctga gtatttttct caaccaatcg tttctaagaa tttgcattac tatgaaaaca
    11341 aaacaatcca cgactcatcg ttgagtaaag cgattcacgc catagaggct tgccgtatcc
    11401 aagaaatgga atgggcgtat catttattcc aagaagcgtg cctgattgat ttaggaccag
    11461 atccgcattc atcagatgac ggaattcatg ctgcttcatt aggagcacta tggctagcag
    11521 ttatatttgg tttttcaggt attacagtaa cgaatggaac tctttctatt tctccaagac
    11581 ttccttcagc atgggaaaaa atttcttttc cttttatttg gaaaaatcac aggttgctat
    11641 ttgttttgac aaaaaaacaa atagtaataa aaaaagagac gaaagaaaca ttacctattt
    11701 ttgtcaatgg agaccaatac cagctggaaa aagaactgat cttggaacgg aggggaaaat
    11761 aatgcgtatt ctgattgcgc cagattcttt caaagaaagt ctgacagcct tagaggcagc
    11821 acaagccatt caatcaggtt ttcaagaagt ttttgttcag gctgaatatg atctgctacc
    11881 tataggcgat ggaggagaag gaacggtttc atcattagca gacagtttaa ggttgaagcg
    11941 gcaaacagca gtcgttacta atgcatttgg caaaaacaaa gaaatccagt acgcttcaga
    12001 cggtcaaact gcgatttttg aaatggcaga aatcgtcgga ttgaaagatg tcccactaaa
    12061 ccaacaacag ccacttaggt tatccacgcg aggagtagga gaactgatac gacaattgtt
    12121 gcaacaaggt gttcgacaca tcatcatcgg cgttggcgga agtagtacca acgatggcgg
    12181 catcggcatg gcagaaggtc taggttatgc tttttacgat caagataatc aaaaagtaga
    12241 agcactggga gaaaatctag gtaaaattgc tacagttgat aaaacgaacg tgcctattga
    12301 gctgattaaa gccaaaatca ctgtcattac tgatgttact aatcttttat gcggaaaaga
    12361 aggcgcttct tttgttttcg gaccacaaaa aggcttgaaa gaaaatgaat tagcagtggc
    12421 tgaccaagcc atgcatgatt tttatatgag atttgcccct caagtaatcg aggcatcggg
    12481 cagtagtgct ggtggtggaa tgggtgcagg actgttagca tttgcttcag ggaaaatagt
    12541 ctctggcatc gactacgtat tggatttact ggagttcgat aaacgggtac agcaagcaga
    12601 cctcgtaatc gtaggcgaag gacgtctaga tggacaaagt ctaaaaggaa aagcgccagt
    12661 tgggattgct agaagagtac cagagggcgt tcctgtgata gctatttgcg gaagtgttgg
    12721 taaaggaagc gaaaaagcag cagatgttgg catttcggcc gtttttccga tccttcctgc
    12781 agctgtgcct ttacaagaag ctttacagca agccactgat aatcttcatc gcacagctaa
    12841 aaacatcgca tctttacttc aagtcgttca ttttcaagga ggtaatcgat gaaaatcaaa
    12901 gcagcactat tcgatttaga tggcgttctt gtagacacag ctcgttatca ttacgaagcg
    12961 tggctggttt tagctaacca gctgtccatt ccattcactg agaaagaaaa cgagcaactg
    13021 aaaggaatat ccagaaccga gtcactagag cgattgctgt catttggaaa aatggaacag
    13081 aaattctcag aaaaagagaa atcagccttt gctgagcaaa aaaacaatct atatcttcaa
    13141 gcgattcaaa aaatggatga aacatccgtt ttgcctggtg caatagctgt tcttgagtac
    13201 ttgaaaaaaa caaacatcaa gatcggattg ggatcagcaa gcaaaaatgc acgcctcatt
    13261 ttagaaaaaa cgaatttgac aagttatttt gatgtcctta ttgacggcac gcaagtatca
    13321 aaagcaaaac ctgatccaga agtttttctc aaaggagcac agcaattgaa tgtaccgcca
    13381 aatgcatgct tggtcattga agattcagaa gctggttgtc aagcagcttt ggctggtaac
    13441 atgcatgtat tgggaatagg tgaaaacata aatcttcctt ctgctgaata tgtgattcct
    13501 gatttaaccg tttttgatca agtacgttcg ttttggcatt tatcagaagc cgtatcacgt
    13561 atataatagt ttccgttttt tctgtcacaa gcaaaacgtt atcctccttt tggaaatcaa
    13621 ggtatgatga gaaaaaaggg ggattttata tgacaaaaaa agcagttttc gcaggcggat
    13681 gcttttggtg tatggtcaaa ccatttgaag agcagccagg gatcatttct gtgacatccg
    13741 gttatactgg cgggcatgta ccaaatccaa cttatgaaca agtaacaaca ggcacaacag
    13801 ggcatacaga agcagtagag atcgaatatg atccagaagt tatttcttat gatcagttgg
    13861 tggaaatcta ttggcagcaa accgatccga ctgacgcatt aggacagttc gctgatcgcg
    13921 gtgattctta tcgtccagtg atctactata atgatgaaga agaacgaaat attgcagagc
    13981 gttcaaaaca agcattgcaa gacagcgggc gattcgataa gccaatcgtg acaaaaatcg
    14041 aaccaagaag cgaattttat cctgcagaag attaccatca ggattattac aagaaaaaca
    14101 agctccatta caatctttat cgagaaggtt ctggacgtgc aggtttcctt cgtcgacatt
    14161 ggggcgtcaa gaaataagaa gccgaaaaag ctacttatgg aacaccgtat atcagaggtt
    14221 gtaaaaccag cattttgagc taatactgag ggtcagctga ttgaacacca tttagtggag
    14281 aggttgtgaa cctgtcgcct agctccaagt cacaaggatt tggtaagaga acactatata
    14341 tcagaggttg tgacaagtcg tttgtcacaa ccttttttca atacctaaga tgaaggacaa
    14401 tcagcaacgc actgctcaaa aaaggaatga aaggcagctt ttccaacttt tttttatgac
    14461 agataaagta aacagaaaaa acggatattc caaacagact agcaagaaac aagatctgaa
    14521 gcaactcttc tagagacaag ccaccactcc aagaaagcat caacagagta tcgccagctc
    14581 cgagatagga ttgaccatag cgaaggcaca caccgcctac gataaacgtt aacagcacac
    14641 tttgccattg gaagacgccg gcctgcaacc aaaaaatcca taaaattgcc caagaacaga
    14701 atagcgtgtg agaatggacc atgaagtaaa ataaatccat caacgaaaat agcaaagccg
    14761 aaagcatcca tatgaacgtc gaaaaatttc ttgcggaaga aaaagtaaag aaccaggcaa
    14821 aaagaccacc acataaggct tctgccaaca aatagtagaa gggcaaccga cacctgcaat
    14881 agcggcagcg aaatcgttgg acgatgatcg agagaatcgg aatcaattca taccaagaaa
    14941 gcgaacgaca gcatttgaca caatgagatc taggataaat aatagagtga cctaacggaa
    15001 tccgctgggc ggttaaacat aaaaatgatc cgatacagca accaatgata aaataaagta
    15061 acacgccatc tccccctttt caaaaccaag atacgcaagc ttaaactaaa tttttgtaca
    15121 tacataaaat ttgaggtgaa aaaatgaacg tttcaatcgg attagatatc ggaaccacac
    15181 aaaccaaagc agttgctttc acagatgaag cccaagaagc agcatccgct tattttcgct
    15241 accctttgat ccaagaaaca caagggatgg cagaacaaga tcaggaattg atctttcaag
    15301 gtgtctgcac cgttataaaa gaagtaatta aacagttgcc ttcagacaca atcattcaat
    15361 ttgtctcctt ctcctctgcg atgcatggcc tgatattgtt ggatgaagag aaaacaccgt
    15421 tatctcgaat gatcacttgg gcagataacc gagcagcgga cgaagcagaa aaactgaaat
    15481 ataccgtgct tggcaaagaa ttttatcggc agactgggac gccgattcat ccaatgacac
    15541 cattgaacaa aatcaagtgg ctgcaaaaca gccaacctga attattcaaa aaaactgctt
    15601 attttgtcgg tatcaaagac tatatctttt atagattgtt tggcgaactg atcagcgact
    15661 acggaacagc ttcaggaact ggatattttg atatccatga attccaatgg gcagaagaag
    15721 tgcttgacta cttgaacatt cgaatagagc aattgccgca agtctttcct tccacctatc
    15781 aagtaacagg tctaccatca aaaacagcag cagaactagg tttgccaaat gatatatcat
    15841 ttgttttggg aggggcagac gggccgcttt ctaatctagg gttaggtgct tttggtgata
    15901 cgatcgccgc tttgaccgta ggaacgagcg gagcacttcg ttttattact actcagcctc
    15961 agttccatcc agaaatggag acgttttgtt atgttctgga tcagaaacat tgggtagtgg
    16021 gaggagcgac tagcaatggt gctggcatct ttgattgggc agggcataca tttttacagg
    16081 aagtcgttca atctgctaaa gaaaaaggag aaaatccata tgatgcactg ctttcggcta
    16141 tcgcttttgt gccagcgggt gccaatggtc tgatttttca tccctattta cttggtgagc
    16201 gagcaccgct ttgggacgca gatgcgtttg gcagttttat cggtttacag cggcaacata
    16261 cagaaaaaga aatgatgaag gcgattctag aagggatctg tctcaacctt tatcggattt
    16321 taaaaggagt ttgccaggaa aatcagctca cagagatccg tgctacaggc gggtttgctc
    16381 aatctccgat ttttcgtcag atgatggctg atgtactggg ttacccgctt gttttcccta
    16441 ctgtcacaga agcatccgca ttaggagctg tgattttagg ctggagaagc atcggaaaga
    16501 tccaaagtat cagtgaagca caggaattga tagaattgaa agagcaagtt ggagtacaag
    16561 aaactgatca taaaaaatat cagaaaatct atccattatt tgtttcgaca caaaagcatt
    16621 tagcacagac ttatcagcaa tttgctcaac tgcgagaaga attagctgat tctgaaaaat
    16681 gattggcttt acggataaaa aagcgaactg aaaatcgttc tcaggtaaat gattttgttg
    16741 tacttttcat tgaaagtcta ctattatttt aagtgtaaga aaatgttagg aggcaagaaa
    16801 gatgaaattc caacaaacca aagaagtatt aaatcaatta gttgcagatc taagccaaat
    16861 gtctgttgtt gttcaccaaa cacattggta catgagagga ccaggtttcc ttacattgca
    16921 cccaatgatg gatgagttca tggatgattt gaacgctcag ttagatgaag tatccgaacg
    16981 cttgatcaca ttagacggtt caccttattc tacattgcgt gaatttgcag ataacacaaa
    17041 aatccctgat gaaattggcc gctgggatcg tactattcca gaacgtttgg aaacacttgt
    17101 tgcaggctat cgttatctag cagatcttta ccaaaaagga atcgaagttt ctggagaaga
    17161 aggcgacgat ccaacacaag atatctttat cgaattcaaa acagccacag aaaaacgtat
    17221 ctggatgatc caagctcatt tgggcaaagc gccagagatc gacgcgtaaa aactgttgtt
    17281 gaacttgacc gaaaaagagg ttgtgaaatc agcgttttga cctgagtatt agagaaacaa
    17341 acgtcaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagctgat
    17401 tgaacaccgt ttaccaagag gttgtaagcc tgccgttcag ctccgagtca caagaaacaa
    17461 ttttaaaaaa cagcttctta tgttttatgg atattgtgac ttgtgaccga ggagttggca
    17521 ggagaacacc gtttattcaa aataaggggt tgtgacaaga cttttgtcac agcctctttt
    17581 tgtttttcat tttgacagga aacgaccctt ttatataaaa gaaagaaaag aagtaaagtt
    17641 tcatataaat ttttcaatca agcagaaaaa cgttgacatt gactagatta gcgggtaata
    17701 tgttaaatgg tattgtttgc cccacaaaga gccccggaaa ctcgaagtgt atgtcaaaca
    17761 tcagaactta aattggagga aaataacgtg cgtacaacat atatggccaa accaggcgaa
    17821 gtagaacgta aatggtatgt agtagacgca actgatgttc cattgggacg tctttcaaca
    17881 gttgttgcat ctgtactacg tggtaaaaat aaaccaactt tcacacctca tttggatact
    17941 ggcgatttcg tcatcgtcat caatgcagac aaagtgaaat taacaggtaa aaaagcgact
    18001 gacaaaattt attaccgtca cagcaactac ccaggaggat tgaaatcaat cactgctggc
    18061 gaattgcgtg ctaaaaattc tcgtcgtttg atcgaaactt ctgtaaaagg tatgcttcct
    18121 aaaaacactt taggccgcaa acaatttaca aaattgaacg tatacggtgg agcagaacat
    18181 ccacatgcag cacaacaacc agaagtttta gacatcacaa acttaatcta ataggaggga
    18241 atttcattgg cacaagttca atatatcggc acaggccgtc gtaaaaacgc agttgctcgc
    18301 gtacgtctag taccaggaac tggcaaaatt actgtaaata aaaaagatgt tgaagaatac
    18361 attccacatg ctgacttgcg tgaagtaatc aatcaacctt tcgctgttac tgaaacaaaa
    18421 ggcgcatatg acgtatttgt taacgtaaac ggcggaggat acgctggaca atcaggagct
    18481 atccgtcatg gtatcgctcg tgcattgcta gaagttgatc ctgacttccg tttagctctt
    18541 aaacgtgctg gcttacttac tcgtgacgca cgtatggtcg aacgtaaaaa accaggtctt
    18601 aagaaagcac gtaaagcttc acaattctca aaacgttaat atcctttata tatcaacgtt
    18661 tctggcactt ttcaattatt tggaaagtgc ttcttttgtt tttaagaaca catttaagaa
    18721 cacatatcaa tcacacttat tcaatcctct ctgaaaagct tcaaaaacag ccttatcaac
    18781 tatatctggt gtgttcacat atatctttgt tgtagaaata tctgagtgtg ttaaagcatt
    18841 tgaaatttga ttcatatctg ctccaccttg tctagctata gttgcataag tatgacggag
    18901 tttgtgtgga gataaatgaa ctaagtcagg atgccttctt ttaattgaat tgattcgata
    18961 gtttaggtaa tcggcatgaa caggacaatt tacttctcca gatggtttag tataagtaaa
    19021 gagatactga tctaacgttt gcttaatttt taatttcaac aattgatcag cttgtaccga
    19081 tttccattca cttagcaatg tcattacaac ttctggtact tgaatgacgg tatcttttcg
    19141 accttttgta aacttttttc tttgatattt atctaaagta tgtttaagac ggaccgtttg
    19201 tttttcaaaa tcaatatatt tccattgcaa agcgtaagtc tcactttttc tatcaccaag
    19261 atacagggta atcataaaaa gtgtgtaatc atgaaaagta agtaaatgat tattaaaatc
    19321 atcatttact gcttctatcc aattagtaag ttcttttgat gatagtgctt gcttaccttc
    19381 ttgtattctt ttttcttcta aagcattttt tttaggtgca gtgatacttt gaataatttt
    19441 agtagttcga ttaaattcga tataatttag tatttctgca atatcgaaca tggaacgaac
    19501 atatcctttt atgattttta tatttgcata ttctttcgat tttttggtta attcatctga
    19561 tattatctca gtattgatat ttagataatt cattgcgtat tcaccaaaca taggtaaaat
    19621 atgcaaccga aatatatctt ttgtattact aatggttata tcactaggag gcctgtttga
    19681 tctaattgtt tgacctaatt catatctagg aagccattta gattcgtaaa attttttaaa
    19741 cgttattttt ccttttaatt ctaatgagtt tgcattctcc tctctcaaaa ctttttctat
    19801 tttcttcttc atatcatttt cggctttaat cgccaattgc ctatttggaa atgttttttg
    19861 atacttttct gatgcaattc ctgttatttc tttaaacctt tttggatatc tcagattagt
    19921 tttatatttc ccatttgaaa gctttgtagg caaaatcctc actccttaaa aaaagtaagg
    19981 cagtatattt cactaatttt agtatatgcg gcgagaagaa aatttcaagt tattttaaat
    20041 gtattcataa agtctttaat atcttctacg tcaaagcgaa ctacaccacc aactcttatt
    20101 actgggaaat tatatatacg aatccactta tctagagtat tattatcgat tcctagaaat
    20161 gcacaggttt gttttttatt caggtagact ctggattcta taaaatcatc tcctaaaata
    20221 gtagttatga aatttttgaa ttgctttggg taagcacata ggaatttcac ctctccccag
    20281 tttacggcga gccttacgga agtatcatta tttgctgcta ttgtcatggc attttgtttt
    20341 cccagaacaa aaagaaatct ctaagtattt gtcgctaatc aggtcatggc gtacttgagt
    20401 ttctagctcg aatagcaggg atcgttattc aaaaattgta tttaattgtc aatgatcaaa
    20461 aatagttt
//
LOCUS       DAZBSA010000049        19631 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000049 DAZBSA010000000
VERSION     DAZBSA010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.049"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(461..1090)
                     /gene="udk"
                     /locus_tag="ACHGMT_001613"
     CDS             complement(461..1090)
                     /gene="udk"
                     /locus_tag="ACHGMT_001613"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFN6835356.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1174..1869)
                     /locus_tag="ACHGMT_001614"
     CDS             complement(1174..1869)
                     /locus_tag="ACHGMT_001614"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFN6835357.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1882..2598)
                     /locus_tag="ACHGMT_001615"
     CDS             complement(1882..2598)
                     /locus_tag="ACHGMT_001615"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFN6835358.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2928..3536
                     /locus_tag="ACHGMT_001616"
     CDS             2928..3536
                     /locus_tag="ACHGMT_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFN6835359.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3861..4184
                     /locus_tag="ACHGMT_001617"
     CDS             3861..4184
                     /locus_tag="ACHGMT_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFN6835360.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4366..5736
                     /locus_tag="ACHGMT_001618"
     CDS             4366..5736
                     /locus_tag="ACHGMT_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFN6835361.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5866..7167)
                     /locus_tag="ACHGMT_001619"
     CDS             complement(5866..7167)
                     /locus_tag="ACHGMT_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFN6835362.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7521..9512)
                     /locus_tag="ACHGMT_001620"
     CDS             complement(7521..9512)
                     /locus_tag="ACHGMT_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFN6835363.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9656..9922)
                     /locus_tag="ACHGMT_001621"
     CDS             complement(9656..9922)
                     /locus_tag="ACHGMT_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFN6835364.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="ACHGMT_001622"
     CDS             complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="ACHGMT_001622"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFN6835365.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="ACHGMT_001623"
     CDS             complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="ACHGMT_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFN6835366.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13266..13829
                     /gene="def"
                     /locus_tag="ACHGMT_001624"
     CDS             13266..13829
                     /gene="def"
                     /locus_tag="ACHGMT_001624"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFN6835367.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14082..14603)
                     /locus_tag="ACHGMT_001625"
     CDS             complement(14082..14603)
                     /locus_tag="ACHGMT_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFN6835368.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14606..15448)
                     /locus_tag="ACHGMT_001626"
     CDS             complement(14606..15448)
                     /locus_tag="ACHGMT_001626"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFN6835369.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15441..16166)
                     /locus_tag="ACHGMT_001627"
     CDS             complement(15441..16166)
                     /locus_tag="ACHGMT_001627"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFN6835370.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16342..16638
                     /locus_tag="ACHGMT_001628"
     CDS             16342..16638
                     /locus_tag="ACHGMT_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835371.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16894..17068
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17206..18798
                     /locus_tag="ACHGMT_001629"
     CDS             17206..18798
                     /locus_tag="ACHGMT_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFN6835372.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18811..19551
                     /locus_tag="ACHGMT_001630"
     CDS             18811..19551
                     /locus_tag="ACHGMT_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFN6835373.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5618 a         4028 c         3724 g         6261 t
ORIGIN      
        1 tagggggtgc gacaaaagag cttcttcaaa aaaataagtc gaaatctcac acaaattcga
       61 gaaataattt tcgtaagttt cttcttattt attgcatctg accacttctg ttacaacctc
      121 tcgttaaacg gtgttcaaaa gcttgcatct acgataataa gcccaaccca acgaaaaacg
      181 tgaggagctg ttttatttgg atgctcttat cgcttgatgc aaagacagct tttttacaac
      241 ctctacttca cggtgctcaa tcagctgacc ttcggtatta gctcaaaaaa cggtaaaata
      301 taaacagcta ttttccgttt cttcttttta atacttaggt caagatgctg ttttcacaac
      361 ctcttgttat aaataaaaaa acagttggct ggtgactgtt caaaaagcag aatccttgct
      421 tttcaaacgg tcgcagtccg ttcaactgtt tcgctgcgca ctatttatgg tttaagaaac
      481 tagctacttt tgttgtaatc aaatcaatag caacgtggtt ttctccgcct tctggaacaa
      541 tgatatccgc atagcgcttc gttggttcga taaattgatg atacatcggt ttaacaactg
      601 tcaaatattg ttcgatcacg gagtccaatg tgcgtccccg ttcttccata tcccgtttga
      661 tacgacgaat gatacgaatg tcatcatctg tatctacgta tactttgata tccatcaagt
      721 cacgcagacg ttcgtcttct aaaatcagga ttccttctag aatgatcact tcttttggtt
      781 cctgaatgat cgttgcctga cttcttgtat gcgctacata gtcatacacg ggtttttcaa
      841 tggctttata gttcagtaag tcgcctacat gctgaatcag cagatcatta tcaaaggcaa
      901 atgggtgatc gtaatttgtg ttcaatcgtt cttcaaaact caaatggctc tgatccttgt
      961 aataagaatc ttgttcaagc atcatgatgg aatggtccgg gaagttgttg aaaatcgcac
     1021 ggctgacact tgtctttccg cttccggatc cacccgtcac accaatgata attggcttgc
     1081 tttttgtcat tgactaaaac ctctttcgta tccgtttttt cattctaatc tattatagcg
     1141 aaaaaacacg aaaatgctat atttttctga aaattaatcc ttttcaattt ccaataatga
     1201 ataaagttcc tctaatgaat cgattcgata agtaggctgg atttcttcga tttctggtaa
     1261 tttatttcta ttgaaccaaa tcgtatcgat ccctgccata tttcctcctt tgatatcaga
     1321 agtcaaagaa tcaccaatga tcacagtttg ctttttatca aaattaggaa tgtgggaaaa
     1381 gacatgatca aaaaattctt tcatcggctt ttgcgctccg acagcttctg agacaaagat
     1441 atccttgaag taatcaagca ttcctgcatc tgttaatcgc cgatgttgcg tgtgcgacac
     1501 accgttcgtc acaatataaa gatcagcatg cggatggacc tttttcagga tttctagact
     1561 tcctggaaca agatcatgac cttggttcaa gtagtggcgg tagcgttttt ccatttgatc
     1621 actatctact gttttcccaa attgctggaa aagtaaccca aaacgactgc ctattatttc
     1681 ttctcgtgat agtctgcctt tttcaaactc ctgccataaa tgatgattca aagccttata
     1741 gctggtttcg atttctggtg tcaatgtgat tccttgttct gcaaacaaag cacttaaagc
     1801 attggcttca gttaattgaa aatctaacaa tgtatcatct acatcaaaca ataaagtttt
     1861 atatttcatc tttttgacct cctatttaaa ataaatactt aactttttca tctctgctaa
     1921 tgtcaacgat cgatactcgc ctactcctag ctgttcatct aattccaatg gtcccatgct
     1981 tagccgtttc aatgcggtca ctttctttcc ttgggctaag aacatctttt tgacttgatg
     2041 gaattttcct tcttggatcg tcaataaaac cgtactttgg tgcttgtctg ctttgagtat
     2101 ttttaaatca gccggcttac attgataccc atcagcgaat acgattccat tttcaaatgc
     2161 tgttacgtct gccggtgtca ccctttcgtt cacaactact tgataagtct ttgaaacctt
     2221 tttttctgga agtagcattt catatcccaa ttgtccattc gaagtgatca gcaatagtcc
     2281 tgttgtatct ctatctagtc ttcccactgg atataatggt tggaaacgat cggcacttgc
     2341 aatcagatcg attactgtcg tcttttctgg atctgataca gctgtaacag ctttttcagg
     2401 tttattgaga agataatagg actcatttgt ttctaagtgt tcacctgcta cttcgatttg
     2461 atgcagtaaa ctatctacat ttcgattttg tcgaaattcg actgtgccat ctatcatcac
     2521 ttgcttcaaa gcaaacagcc gcttcatttc cttgcgtgtc gtttctaatt tttcttctat
     2581 taatttatct aaacgcatgc tgtttccttt cttattacta ctaattaatt ataccttgaa
     2641 tattcttgct tgcaaataca tcatcatagt tattccttga cttaattaac agttcacgtt
     2701 acggttaaat agtaaagtga attggatttc tcatacacag cgtaatgacc tggtggagaa
     2761 aagttgaatc ctgactttct aaggggagat gcgcgcatct ctccttttgc gcttttttaa
     2821 gcgccctgcc ttcgatgatg tgcacgagat tagcctttag aattcactta aataaaatct
     2881 gtccgttacc aaagcagaca gtcacatttt aaggaggaat tttggttatg tcagtatcat
     2941 tagaagtaag taaaagagaa gtacgtcccc gttcattaag aaacaaactt cgtcacgaag
     3001 ggaaagttcc agctgttgtt tatgggtata aagtagaaag tacccctatc tcattcgatg
     3061 gtttgacact aagcaaattg ttgcgtgaac atggagcgaa cacagtcatc acgatggaca
     3121 tcgatggaca aaaagtcaat acattggttc acaagatcca aacgaacact tttaccaatc
     3181 atttcgaaca tgcagaattt ctatctgtaa acatgtcgga agaaacagaa gtcgaaacag
     3241 aagtcgttct tgttggcgaa tcagtgggtg ctaaatctgg tggagtgtta gctcaaaact
     3301 tgtatacagt agtcgtatct gctacaccag acaaattgcc tgaacgaatt gaagttgacg
     3361 tgacaaactt ggaattaggt gattctatta cagtagccga tcttccaaaa aacgacgatt
     3421 acaaattcgt gaccgacagt gaagagcaaa tcgctgctgt agtcgctcaa agtgaagaag
     3481 aagaaagcac aggtgaaggt ggcgaaccag aagtgatcgg ttcaacagaa gaataatact
     3541 gaaacaatca ggtggtgtta gaagcggcgg gtcagcagct tttaacacgc cttttttatt
     3601 tctaatcgtt tattttcata tggggcttcc acttttgtaa atggctttac aaacgaaaaa
     3661 tcctaattta ccaagctttt cctcgcatac ttttatcgta cctttataaa aatttatact
     3721 ttgaaaaaaa gtattcaatt attgttgcat ttttcctgat tctttattag aatgaaccct
     3781 cgttgaagta agtatttcta gtttatccta ggattaatta taaaaataaa acattcgttg
     3841 attcgaagga ggttaaacag atgtttgaaa gttttgatag cagccgcaac cgttacgctt
     3901 cagttggtgt cgtatcaagt ttgccagatg aattgattga cagtatatgg tttattattg
     3961 acctagatct taagggagtc attccattag aaaatatatt aacctttgat ttgatcgaaa
     4021 atcgtggaaa agtgacgatg catttttctc aagcaggaag caatgtggaa atggatattg
     4081 atttgccgtt tgcctattcg aaccaatttc ctgctcaagt atacgcctat gatgacggta
     4141 cgagagaaac gatcctttta ccaagtgaga ttagacaata ttaaggtgat agataaacgt
     4201 ggatgtccct tattttacag accaggccaa gttgttgatt ctttgactca attcttggct
     4261 tctttttatg gagtttcata aattatcatt gatttctaat tggtattctc aggataaacg
     4321 ctatatacta ctaactaaat tttaaagata taaggagttt aaaagatgcg cgatttaaca
     4381 aaaggaagcc ccgctaaact gattttgatg ttcactgttc ctttactgat cggaaatgtt
     4441 tttcagcagt tctacaatat gatcgatatg atcatcgtgg gtcagacgct aggtaaaaat
     4501 gcgctggcag cggttggtgc aactggaagc ctgacatttt tgatcattgg ctttgcccaa
     4561 ggtttaacag ctggtttagc cattattacc gcacaacgtt acggtgcaaa agattatcgg
     4621 ggtctgaaga aaagttttgc tgctagtgtt gtcattagtc ttgtcgtcac tgtgatttta
     4681 actgtcctaa gtctcctgtt tattcgtccg atgcttcaat tgatgcaaac tcctcctgaa
     4741 atacttgatc aagcacaaac atttatttca attattttat tagggatctt tgcatcagtc
     4801 agtttcaatt tgctttctaa tgtcattcgc gcacttggtg acagccgcac accactattc
     4861 tttctgatca ttgcggtcat catcaacgtc gtacttgatt tgatctttat tatctatttt
     4921 ggcatgggtg tggaaggtgc agctattgct acagtaatcg cacaagtttc ttccagcgta
     4981 ctgtgtctag tgtatatcaa gaaaaaaatg cctttgttac agttacgcaa aaaagatttc
     5041 aaatttgaaa aagaagagtt tgctgtacac ctaaatgctg ctttgccaat ggcgtttcaa
     5101 tcttcgatca tcgctgttgg tgctatcgtg ttacaagcag cactaaatag tctagggaca
     5161 gatgtcgtag ctgctcaagc cgctgctggt cgtatcgacc aatttgcaaa tcagccgatg
     5221 atgtcatttg gtattgcaat ggcaactttc actgcacaaa actatggtgc gaaagaatat
     5281 ggccgaatct tgcaaggggt acgtcaaact ttactgatga gtgtcggatt tagtctctta
     5341 gcaggtgcta tcgtcatttt cttcggtcat ttctttgttc aattgtttgt tagcccatca
     5401 gaaacacgtg tatttgaatt agcacaaacc tacttcaata tcaatgggtc tctttactgg
     5461 atcttagcag tcctgttcat tttacgctac acacttcaag ggcttggaca aagcaagatc
     5521 cctacgatcg ccggtatgat ggaattgctt atgcgttctt ttgcagctat cgttcttaca
     5581 ggaatgtggg gatacccagg agcagcagca gcttctcctt tagcttggat cggttctgtt
     5641 gccgtgttgc tttactctta tatgcgttcg atgaaacaat tgaaacacat gggtgaagag
     5701 gaacatttca cattagaaca tgcagaagca agatagcctt gattaaaaga aatcgttgcg
     5761 gctaaaaatg aaaactttta ttgaaaatat cttcttcgtt taccgaaaac aggtttttta
     5821 gataacaaaa aggctgtgac aaaaaatgtc acagcctttt ttcttttatt ctgtttttct
     5881 ttccgtggac tctactttga aataatcgat atgtgcatgc tggtattcta ataccgtgaa
     5941 acggaatgat gcaatctcaa tgattgttcc tgcttttatt tctttatatc ggctagaagc
     6001 aaagcccgca agtgtagtaa aattcgtttc ttcaaactct ggaatctcta catgaaaata
     6061 acgctggaaa tcatctaatg ttagtttgcc ggagatgata taagagccat ctgactgttc
     6121 cgctatcaat gcttcgtcca aatctgtctc atctcggatt tccccgacga tttcttctaa
     6181 aatatcttca atcgtcacta agccgcttgt ccctccgtat tcatctttta agatagcaat
     6241 atgcttaccc ttttttcgca tgatcgtcaa taattttttt atagggatcg tttcagttgt
     6301 tacgatcggc tcttcaatca actgttctac tgtcagctgg tcatctttat aagattgctt
     6361 gatcaaatct ggcaaggtga cataacctaa aacattgtct tttgattcta agataaccgg
     6421 atagcgagta tgtccttgtt caatcgaagc ttttatcgct tctttgacgg tttctgaagc
     6481 ttcgaatact tgcatggacg tacggtctac ttgtatatct tttgccaagg tatcatcaaa
     6541 ttcaaacacg ttttccaaat aataatattc ttctttggtc aactctcctt tttcataaga
     6601 attcaatgcg actaatgtca gctcttcttg agacaatgtc tcttctcctt cagatacgag
     6661 acgcacacct aatagcttcc ctagccctgc agctgaatga ttcaataccc aaataaaagg
     6721 aaacattact ttataaaaat aatgaagtgg tttaactacg aacaagacga ccttctccgt
     6781 attgacgata ctatagctct taggaagcag ttcgccaata accacatcga tatacgtaac
     6841 taaaataaat cccagcgcca cagagattgt actgctaata gccgaactaa aaggtagttt
     6901 accgatcact ggttctagta aagccgcgac agatccttca ccgacccacc cgatgatcaa
     6961 accggttagc gtaattccta attgactagc tgataaatag ttatctaaat ggtgtgtaac
     7021 atgaagagct agcttggcgt tctttacatt ttccttttct agttgttcca atcttgatcg
     7081 tctgattttc actaaagcaa attcacttgc tacaaaaaat gaagtaattg caatcatcaa
     7141 aaataaaata atgaaattga taaccatatt ctcccgcctt tctctttttc tattgtaccc
     7201 tttttctaca aaaagattga aaataatgcg tattcatatt taggaataaa aaaggagatg
     7261 cggcattttt cagccacatc tccttatcac ttcaaaatca ttgcgttact tgttgctaaa
     7321 cagctttttc atgacctctg ataaaaacgg tattcaaaag cttgcatcta cggcattaag
     7381 cccaaacaga tgaaaaacat gagaagctgt ttttatctgc tcgttcttac tgcttgttgc
     7441 agtacagctt tttcatgacc tctgataaac ggtgttcaag tagctgacat tcgacattag
     7501 ttcaggtcaa agcgctactt tcacaaccgc tctttcaacc atgttttccg agtatttcca
     7561 atgataagcg gaacagtttc atgttttact tcgctgtact ctagcccaaa ccatgtttct
     7621 ggcgaggtag cgattttttt gattgccagt tttgcctgca tgatttgacg ttgccatttt
     7681 cctagagttg tcacattgga aactacttca tcaatcagga caaattggaa atcgccaact
     7741 tctcgccctg gtgtcagaga gtatctttgt ggctgttttg gcaaacggcc ttgtttcatc
     7801 aaatcatgga tgacttggcg aacatatacg ttgatttcct gttggaccct gaaacctaag
     7861 aacagattca cttggacaat aaaatcagtg tccatcatat ctacttgata ttctttcgta
     7921 tatggctcat ccgtcacttc cacatgaaca aaccaataga ctcgtgctct tttaggccgt
     7981 ttatctaaaa tcgaatagat gaactgtcgg ccaactttat cttcagccat atcaggaacc
     8041 aagaagacca catttgtctg atagattggt aaagaagtat catcacgtaa ttctctaagc
     8101 tgcgggatat aatctctcaa agacacatcc tcggttgacg attcgcgaat aatgttccct
     8161 tgttcccaaa taaacatcac gactaagatg atcaaaccaa tcagaacggc tacaaaaccg
     8221 cctcggaaga atttgacaac acttgagata aagaaaacga cttctatcgc accgaagaaa
     8281 aaggtaacta aatatgcaag ccaacgagaa tgcccatttt ggataagata aaagtaaagc
     8341 agtgctgttg tcatcaacat cgtcacagtg atcgatagac cgtaagcagc ttccatatga
     8401 tgagacgttc ggaatgtgac aaccactaga gaacaggcga tccataaaat cagattgact
     8461 gctgggatat acatttgccc gatactgttc ccaggataca tgatctttaa ccgaggcagc
     8521 agtttcaact tgattgcttc tgaaactagc gtaaacgaac ctgtaatcaa tgcttgcgaa
     8581 gcaatgactg cagcgactgt tgcaaaagca acgccgatca cgatccaact ttgaggcatc
     8641 atttggaaga atggattcag gttcttgatt tgctgtgctt cagggttttg ataaacattc
     8701 aacaaccacg cagcctgccc aaaataattg ataaccaaac aaattttgac gtaaggccag
     8761 ctcagacgaa tatttcgttt gccgacatgt ccaagatcgg aataaagcgc ctctgcacct
     8821 gtcgtagcca ggaaaatatt tccaagaatg aacaagccta atttgttatc tgcactaaaa
     8881 agcagctgga tagcataata aggattcagt gcacggataa ctgtccagtc ttgactgaag
     8941 ttcattaatc ccattacgcc taaaaaagta aaccaagcga acatgattgg tccgaatgct
     9001 tttccgacaa tatctgtacc aaagcgttgg accatgaata aaacaaaaat aataactaaa
     9061 gtgataatga cgatcacgtt ctggttatta ccgaaccggt caaaaaactg cggaattcct
     9121 cgcaagcctt caatcgcagt cgttaccgtc acagcagggg tcaatacccc atctgctagt
     9181 aaggcagctc ctccgatcat tgcaggaatg atcaaatatt tcccaccttt tcggacaagt
     9241 gtgtagagtg agaaaattcc accttcacca tggttatctg cattcaatgc aatcacgaca
     9301 tatttgattg ttgtcagtag agtcaatgtc caaaaaatca atgagactga tccgataaca
     9361 aaatcaggat tgattcctga caatccgcca ttatcttcaa caatcgattt cataacgtaa
     9421 agcggacttg tcccgatgtc tccgtagacg actcccatcg ctacaagcag gccagccatt
     9481 gatatctttt tggtattgtg cgttgttgcc acgtttttcc ctcatttctt gttatatgga
     9541 tatcctttag gtaagcttga atgatagatg atattcttat ccatgacacc cgctttccta
     9601 aattgcttct aattatccaa gtaacacact gaagtgtcaa cttatttgaa taaaattatt
     9661 ctttctttgg acgttctctg atacgtcttg ccacaaatga ataaagagca aggcaaaaaa
     9721 ttgcacccaa taatgctggt atcaaataaa aaccgccgag ttccggtccc agtggtccta
     9781 gtaaaaattc gccgagccaa ctacctaaaa aaccgataat gatatttcca gatacgcctc
     9841 ccgggacttc ctttcctaaa attgcgccag ctatcaaacc taatataccg ccgataaata
     9901 acgaccataa aaaattcagc atctcctttc cctcctttat ccatatttct cacttttatt
     9961 ttacgcttta ttcagaagga ctccaaaaat atgcgccaag aaactccaga aaaaaagaag
    10021 ttgtaacaaa agtgttcagc ttatttccgt agaagctgct tctggaacac cgtgaatcag
    10081 aggttgtgaa atcagcggtt tgacctgagt attagagaaa caaacggaaa aatagttcct
    10141 catattttgc cgttttttga gctaatatcg agggacagct gattgaacat cgtttattgg
    10201 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa cagccaagta aaaacagctc
    10261 ctaatgtttt taacttggct ggacttatta ccgcagatgc aagcttttga acaccgttta
    10321 ttcgaaaaaa aaggactgtg acacggcttt tgtcacagtc cccttttttc gaataattat
    10381 tgtccccctg tcaccttcac gatcacaagt acattattcc tatgaattaa attatttttc
    10441 ttcatttttc gttgaaccat cttcgttcaa cattgctttt cttgacagat ttacgcgtcc
    10501 ttgtttatcg atttcagtta ctttcacaag gatctcatca cctagtttaa caacatcttc
    10561 gactttattg acacgatcgt ttgctaattg ggaaatatgg acaagtccat cttttccttt
    10621 gatcaagttg acgaatgcac cgaatttttc gatacggaca actttcccta gataaacttc
    10681 accaactttg acttctttgg tcaaatcttc gatgatcttg atagcttttt tgatcatttc
    10741 tgcatcagct gaagcaatac ttacttttcc atcttgatcg atatcgattt tcacaccagt
    10801 ttcatcgatg attccgttga tcgtatcgcc gcctttaccg ataacatctt tgattttagc
    10861 aggttcgatc tggatcattt cgatttttgg tgcatattgg cttaattctt cacgtggagc
    10921 agcaatcgtt gaagtcaatt cttctaagat ttccatacgt gcttgttttg cttgagttaa
    10981 tgcttcagtt aggatttgtt ctgtgattcc ttgaatcttg atatccatct gtaaagcagt
    11041 gataccatct tttgtaccgg caactttaaa gtccatatcg ccaagatgat cttccagccc
    11101 ttggatatca gttaggatcg tatagttttc accatcagaa acaagtccca tcgcaatacc
    11161 ggcgactggc gctttgattg gtacacctgc atccatcaat gctaatgtgc cagcacagat
    11221 acttgcttgg gaagaagaac catttgattc caatacttct gctactaaac ggatcgtata
    11281 aggaaattct tcttctgaag ggattacttg tgctaacgca cgttcaccta atgcgccatg
    11341 tccgatttcc cgacgccctg gagagcctgc acgtcctgta gaaccaacag aaaattgagg
    11401 gaaattgtag tggtggatga atcgtttgct gacttccacc cctaaaccat caatgatttg
    11461 gtgctctcct agaggtgcga gtgtacaagc tgataaagct tgtgtttgtc ctcgtgtgaa
    11521 caaacctgat ccatgtacac gtggcaataa gctgacctct gatgacaatg gacggatttc
    11581 atccaattta cgcccgtcag gacggatctt atcgatggtg atcaattcac gtacgacatc
    11641 tttttcaaga tcttctgtga tttgtttaac ttctttcaac aagtgatctt catcttcatg
    11701 acctgcaaat ttctcagcat aagcttcgcg gatatcgatc ttcacctgct cgatattatc
    11761 ttcgcgagca agtttttctt cagtcattac tgctgctttc attgtagcgt aagaagcatc
    11821 aaagatttct tttttcaata cttcatcaac ttgcagtaaa gtcacttcca ttttttcttt
    11881 ccctactgct tgaacgattt cttcttggaa agcaactaac tctttgatag cgtcaaaacc
    11941 aaacagcaat gcaccaagca tgtcttcttc tgaaacttct ttcgcgccgc tttcaaccat
    12001 attgatggct tgtttcgttc cggcaacagt caattcgata tctgtttgtt ctgcttgttc
    12061 tactgttgga ttcaagacgt actcgccatt tacacgacca acttctacgc ctgcgattgg
    12121 gccgtcaaat gggatatctg agattgatag tgctaaagaa gaacctagca ttgctgccat
    12181 agctggcgag cagtctgttt ctacactcat cacgatgttc gttacttgga cttcattacg
    12241 gaagccttct gcaaacatcg ggcgaattgg acggtcgatc aaacgggcag tcaatgttgc
    12301 ttccgtactt ggacggcctt ctcgtttgat gaaacctcca gggattttcc ctgctgcata
    12361 cattttttct tcataattga tcgttaatgg gaaaaagtct gtatcttttg cttctttaga
    12421 ggccacagct gcacttaaga ctactgtatc tccataacgg actaaaacgg ctccgtttgc
    12481 ttgttttgct agctgaccga cttcgatttg taagggacgg ccgccccatg tcgttttgaa
    12541 tacttgtttt tctgacataa cttctccttt tccttctcgt gaaagcaaga cgctactaaa
    12601 caaatgaaaa ctgccgaaag ccgtcttttg agcagctttc atttggttag taggagtcca
    12661 tctagcttga tcacggaatt ttacttaatt gatatacgag taaaaaaagt gagattcatg
    12721 tgaatcccac tctttttgac taaaattaac gacgcaagcc taaacgtttg atcaattcgc
    12781 ggtaacgttg aacgtcagtt ttacgtaagt aagctaacaa gttacggcgg tgaccaactt
    12841 ttttcattag gccgcggtat gaatgatggt cttttttgtg cacgcgtgca tgttcattca
    12901 agtggttgat ttcttcagtt aatacagcga tttgtacttc tggagaacca gtatcccctt
    12961 catggcgagc gtattcgttg atgatttcat tcttgcgttc ttttgaaatt gccatttctt
    13021 tcacctcttt cgattattcc cctgcgactg agtaaaacgt tggtgattcg cttaaccaag
    13081 tggcaggtca tgtgcctaga gcacacagct atactttacc tgaaaccggg ttgaaaatca
    13141 agtagttaat cattaagaaa aggtagtttt caaacaatct tactactact tttccatttt
    13201 tgtggtaaac tataatgcga aaatagactt gaaacacagt cttttaagaa aggggtacat
    13261 attccatgat tacaatggat gatatcattc gtgaaggaaa tccgacctta cgcgaagttg
    13321 caaaagaagt ttctcttcca ttaagcgaag aagatatctc tttaggaaaa gagatgcttg
    13381 agttcttaaa aaacagccaa gatccaatca aagctgaaga actacactta cgaggcggtg
    13441 ttggcttagc tgctcctcaa ttagatattt ctaaaagaat cattgctgtt cacgtaccaa
    13501 gtcctgaccc agaggcagat ggaccttctc taagtactgt tatgtataat cctaaaattt
    13561 tgagccattc tgttcaagat gcttgtttag gggaaggtga aggctgttta tctgttgatc
    13621 gagaagtacc aggatatgtt gttcgccatg ccaaaatcac tgtgtcttat tatgatatga
    13681 atggtgaaaa acacaagatt cgtttgaaga attatgaatc gatcgtcgtt caacatgaga
    13741 tcgaccatat caacggtgtg atgttctatg accacatcaa cgatcaaaat ccatttgcac
    13801 taaaagaagg cgtattggtg atcgagtaga gaattggcag gttccttcga tactatgcta
    13861 caagaaagct ttataaaaaa ggggagtgct caaaagctgc acttcccttt tttatgtttc
    13921 tccactaatc gaaatttaga tagacatcta gaaatcgcaa ttccaaaaaa ttctgtgtat
    13981 tggatacgca tcttttctta ttttttctta tttcttccat atgggtgttt atctcagaga
    14041 tgatagaata gtttctagga taaaaagtag cagctacgat gttatccttc gtagaggatt
    14101 cctgcttcag ctagtttttg tttgatgttt ttcagcatat gcttttctgg gtagcggatc
    14161 gtatgcagat ggatgccgcc tgttaattcg gataacaaag tagcattact tttttgataa
    14221 cgtctcataa aggaatcgat atcttgtggc gttttgatgt gcaaagttcc tgctaactca
    14281 ccgtaaaggg gatgttccac aattacatca atgatttcac caccattttc aacgattagt
    14341 ctcagttctt cttctgtttg atcttttcca tgctggacag cgattttact gatgtgccca
    14401 gtattggttt cttctgtttc gaggatataa cctcttgctg ttgccacgat acttttacct
    14461 gcagctcgta gtaaagcaat atcgcctaca atgatctgac ggctgactcc aaaagttttc
    14521 gcaaacttgc tagcactcac tggtttttct gtatttgcca aggtttgcat gatttgtttt
    14581 cgccgtgtct ctccttcgat cacacttatt cctccttcat tttgttttgg tattcgcgcg
    14641 agtgtttcat caaatcaaca tacgtgtctg ttattgtttc cttatatccc ttatttttca
    14701 gcagactaac aactttatcc aatacttgct gctctctttt tgcatcataa actgcttggt
    14761 tttcttcttt tttcacttgt cctattttag ttaccgtatc cattcttttt tctaataaag
    14821 caaccaactc tcgatcgatc atatcgatct tatgacgtaa tgtttcaaga gactcttctg
    14881 ttagttcttc ctctactgtc ttgatgatac gagcaggatt tcctgcaatc actacattgt
    14941 cgggaaaaga tttggtcaca accgaacctg cgccgactac tacgttgtcc cctagagtga
    15001 cacccggcgt gacgataact cctccgccta accaaacatt attaccgatt ttaatgggtt
    15061 ttgcgtactc cagaccgctg ttacgtttca ctggatgtag tggatgcgtc gccgtgtaaa
    15121 gctggacatt cggagcgaac atgcagttat caccgatttc aatcgtgctg acatctaaaa
    15181 atgtacaatt gaaatttgca taaaaattct ccccaacaaa tatattatat ccgtaatcaa
    15241 aattgataac tggctccata tagatttttt tccctgtacg gccaaaagtc tctttcaaca
    15301 attgggatcg cagttccgct gattctgctt ggttgatgat ttggctttga cttcttgcaa
    15361 acatacggtc ggcaaccagc tcaggatcat tggcaaaata aagctcgcct gcaatcattt
    15421 tttctttttc ggttttgaat tcagtcataa tctgctccta attcttgtat ttttttgaaa
    15481 acaaccgctc cgtcttcttg aataaacgta taaggctccg ctaaataaac aggcgtttga
    15541 ttgattacta agattttggc tgacggctgg cgaaactgta ccagatcaca aaacggatga
    15601 acttgaaaac tcgttccaac aatgacgatc agctctgctt ggctgacggc ataagccgcc
    15661 tgctccatga ctccatcttg aaacccttca ccatataaca cgatgtctgg gcgtatttgt
    15721 cctccgcatg cttgatgttt gtcgctgtga agatattctt tccagtctac cgtttgttga
    15781 cattttcgac aatgacattg atacagactc ccatgaaaat tgacgagttg cgggctttta
    15841 gcctttgcgt gcaaaccgtc gatgttttgg gaaacaaccc aaacattttt ttctttcgct
    15901 agatgtgcta tttccaaatg aatgatattg ggttttgctt caggatgata aatctttttg
    15961 acaaattgat agaatttatc cggttcattc accattgctt gatgactcag caagtattcc
    16021 ggctgctcca tcccgtggta gattcctgac aaagatcgat agtctggcac tccagaaggt
    16081 gtggataccc ctgcaccggt caaaaatgta atttgctgat aagctaatat ctcttttgct
    16141 gcttcgtcta ctgttaattg tttcatgttt tgtcacctca ctttccatta tagcccatac
    16201 tcgaacaaat ttattgttga aaagtcagaa aaatgggaat aaaaaagtgt tatgggcatt
    16261 tgagcaattt atcacaatgt tcactgacaa aagaatgaaa acgtgttaca atactgctgt
    16321 ataaaagtga ggtgaatcga tatgttacca ttctttattt attggggcgt tattctggct
    16381 tgtatcgcct ggttagcgtt aagcgtttat ttctctgttt tttatcttgt acgcaaagaa
    16441 aatggaaatc tttgggcttt tgctttattc aacgtgattg ccgcaattgt tttagccatt
    16501 acattagccg tatatcgaac atggggctgg ggaatcaccc aatactcaag tttgatttat
    16561 ctaatattag ccatttatgg cgtcgttgtt cttttgcaag cgatcctagg acgtgagcca
    16621 aagaaagcgg cagcttaaat gatcgagatg gaactggaca caggaaattt tctgttctag
    16681 ttcttttttt attgtattac atacattttt gtgtataatt atactatcgt ttgcaatgga
    16741 tagatgattc acattttgat tgttttttcg gtaaaagtaa agatttctat atttatcagt
    16801 attttttcaa aaaccagcag ttatagattg acaaagcctc tcgagtatcg taaacttttt
    16861 ttgtataaat aactgaatac gagatgcctc aatcaatgag gtagaggtcg cgagaatcat
    16921 taaccctgtg gagtgaagca aacatggaga agcaggaata gggaggatcg ccgaaatgtg
    16981 cacttttttg cttggagtgt atgttgggac gcaagttaag agcttgcgga ctgtcttagc
    17041 agtgatgcta agttgcgcta tgttttgtga gaggtttatc accttatgga attttagcgg
    17101 cctttttata tctataaatc ggtcgctttt tattttttca gataaagctt taccgcattt
    17161 catctagacc gctcgtgtat catataatca gaaagggaga aaaaaatgaa aaaaaatatg
    17221 gtttcagttg ccgtactatt tgcttttgtc ttctcactat tcagtgtggg atctctggca
    17281 aatgcagatg aaaaaacacc gagcgatgag tttcgcgtag gtatggaggc tgggtatgca
    17341 cccttcaact ggtctcaaca aacagatgag aataatgcct tgccaattca aggccaaaat
    17401 agttacgccg gcggttatga tgttcaaatc gctaaaaaag tagcagatgg tttagggaaa
    17461 aaattagtca tcgtccaaac gaaatgggat ggtctggctc ctgcccttca atcaggcaag
    17521 atcgacgcga tcatcgccgg aatgagccct actgctgaac gacgaaaaga aattgatttt
    17581 accgatcctt attacgaatc acaattagta gtagtagttc aaaaagatag caaatatgca
    17641 aaagcaaaaa gtttagcaga tctttctgga gcaaagatca ctgctcagtt aaatacattc
    17701 cactatactg tgatcgatca gataccagat gtgcagaaac aacaagcaat ggataatttc
    17761 tcagcaatga gaacagcttt agcttctgga atgatcgatg gttatgtcag cgaacgtccg
    17821 gaaggtgtaa cggcaactag tgtgaacaaa gacttggaaa tgcttgaatt cagcggtgca
    17881 gatggcttcc agacaaaccc ggaagatgtt cagatttctg ttggtatgcg taaaggcgac
    17941 ccggatatcc aaaaagtaaa ccagattctt tctggcatct ctaacgatga acgaaccaaa
    18001 attatggacc aagcagtcaa agatcagcct gctgcaactg atagtgaaga aaaacctggc
    18061 atcatttctg atttcactac gatttggaaa caatacggca atatgttctt acgaggtgct
    18121 ggacttacct tgttcatcgc attgatcggg acagttgtcg gtactacact tggattactg
    18181 attggtgtct tccgttcggt acctgaatca ggaaatgcac tgactcgctt cttccaaaaa
    18241 atcggaaatg cacttctttc tatctatgtc gaagtattcc gcggaacacc gatgatggta
    18301 caagcaatgg tcatcttcta cggtctggca ctcgcttttg gcatttcatt gaaccgaacg
    18361 attgccgctt tgtttattgt ctctctcaat actggagctt acatgacaga gatcgttcgt
    18421 ggggggattt ttgcagtaga tgaagggcag ttcgaagctg cgcaagccat tggtatgacc
    18481 catggccaaa cgatgagaaa agttgttatt ccacaagttt tacggaatat cttgccagca
    18541 acaggaaatg agtttgttat caatatcaaa gatacagcag ttttaagtgt aatcggcgta
    18601 gctgatttgt tcttccaagg aaactctgct tccggtgcga atttccaatt cttccaaaca
    18661 tttacgatcg tcgggatcat ctacttagtc atgaccttta cgatcaccag aatcctgcgg
    18721 gcggtagaaa agaaaatcga tggtccttca gcttatacca agcttgaaga aatcgacaat
    18781 tcaaatttgc aggaataagg aggaagaatc atgaatgaaa aaattattga aatcaaacat
    18841 ctaagaaaaa gcttcggtga aaatgaagta ttgaaagata tcagcgtaac agtcaataaa
    18901 ggtgaagttg tgacgatcat cggttcttcc ggttctggta aatctacttt cttgcgctgt
    18961 atcaatttac tggaaaaacc aacaggcggc gagatcattt ataatggaga aaatgtatta
    19021 gctcctaaat ataatttacc taaatatcga acgaatctag gtatggtttt ccaatccttc
    19081 aacctgttca acaatatgaa tgtcttggaa aactgtatgt ctggtcaaac aactgtcctg
    19141 aaacgagata aagaaactgc caaaaaagta gcaatcgaaa atctggaaaa agttggtatg
    19201 gatcgttata tcgaagccaa accagcacaa ttatctggtg gacagaaaca gcgtgtcgca
    19261 attgctcggg cattatcaat gaatccagat gtattgctat tcgacgaacc tacttcggcg
    19321 ttagaccctg aaatggttgg cgaagtgctt aatacgatca aagacttagc acatactggc
    19381 ttgacaatgc ttatcgtgac gcatgagatg gaatttgcaa aagatgtatc tgaccgtgtc
    19441 atttttatgg ataaaggtgt gattgcagaa gaaggtacac cagaagatat ctttgtccat
    19501 ccgaaagaag aacgtacaaa agaattcctt tcacgtattt taaatgctta ggatcaatag
    19561 ctgattggga caaagagctg cggtgtttac cgcagttctt ttttatgaat tactcataaa
    19621 aggtaaattt c
//
LOCUS       DAZBSA010000050        19345 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000050 DAZBSA010000000
VERSION     DAZBSA010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.050"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            97..663
                     /locus_tag="ACHGMT_001631"
     CDS             97..663
                     /locus_tag="ACHGMT_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN6835374.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            808..895
                     /locus_tag="ACHGMT_001632"
     tRNA            808..895
                     /locus_tag="ACHGMT_001632"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:840..842,aa:Ser,seq:cga)
     gene            1231..2205
                     /gene="bsh"
                     /locus_tag="ACHGMT_001633"
     CDS             1231..2205
                     /gene="bsh"
                     /locus_tag="ACHGMT_001633"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFN6835375.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            2530..4425
                     /gene="asnB"
                     /locus_tag="ACHGMT_001634"
     CDS             2530..4425
                     /gene="asnB"
                     /locus_tag="ACHGMT_001634"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFN6835376.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(4620..5066)
                     /locus_tag="ACHGMT_001635"
     CDS             complement(4620..5066)
                     /locus_tag="ACHGMT_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFN6835377.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            5294..5443
                     /gene="rpmG"
                     /locus_tag="ACHGMT_001636"
     CDS             5294..5443
                     /gene="rpmG"
                     /locus_tag="ACHGMT_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFN6835378.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            5531..6049
                     /locus_tag="ACHGMT_001637"
     CDS             5531..6049
                     /locus_tag="ACHGMT_001637"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFN6835379.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(6126..7082)
                     /locus_tag="ACHGMT_001638"
     CDS             complement(6126..7082)
                     /locus_tag="ACHGMT_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN6835380.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            7248..8054
                     /locus_tag="ACHGMT_001639"
     CDS             7248..8054
                     /locus_tag="ACHGMT_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6835381.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8051..9040
                     /locus_tag="ACHGMT_001640"
     CDS             8051..9040
                     /locus_tag="ACHGMT_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFN6835382.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            9037..10041
                     /locus_tag="ACHGMT_001641"
     CDS             9037..10041
                     /locus_tag="ACHGMT_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFN6835383.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            10173..10715
                     /locus_tag="ACHGMT_001642"
     CDS             10173..10715
                     /locus_tag="ACHGMT_001642"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFN6835384.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            10735..11433
                     /locus_tag="ACHGMT_001643"
     CDS             10735..11433
                     /locus_tag="ACHGMT_001643"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFN6835385.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            11454..11666
                     /locus_tag="ACHGMT_001644"
     CDS             11454..11666
                     /locus_tag="ACHGMT_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFN6835386.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            11666..12628
                     /locus_tag="ACHGMT_001645"
     CDS             11666..12628
                     /locus_tag="ACHGMT_001645"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFN6835387.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            12646..13041
                     /locus_tag="ACHGMT_001646"
     CDS             12646..13041
                     /locus_tag="ACHGMT_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFN6835388.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            13204..13569
                     /locus_tag="ACHGMT_001647"
     CDS             13204..13569
                     /locus_tag="ACHGMT_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFN6835389.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            13566..15377
                     /locus_tag="ACHGMT_001648"
     CDS             13566..15377
                     /locus_tag="ACHGMT_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFN6835390.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(15439..15606)
                     /locus_tag="ACHGMT_001649"
     CDS             complement(15439..15606)
                     /locus_tag="ACHGMT_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFN6835391.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            15855..16844
                     /gene="galE"
                     /locus_tag="ACHGMT_001650"
     CDS             15855..16844
                     /gene="galE"
                     /locus_tag="ACHGMT_001650"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFN6835392.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            16856..18340
                     /gene="galT"
                     /locus_tag="ACHGMT_001651"
     CDS             16856..18340
                     /gene="galT"
                     /locus_tag="ACHGMT_001651"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFN6835393.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            18364..19344
                     /locus_tag="ACHGMT_001652"
     CDS             18364..19344
                     /locus_tag="ACHGMT_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN6835394.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
BASE COUNT         6125 a         3296 c         4067 g         5857 t
ORIGIN      
        1 aaaatataac gttttgagta aaatgtttgc aaaaaatgct aaaaataggg tacaataaaa
       61 caacatatac tgcagtaaag aagtggggga gttttaatga gaagaaaagt ctataccaag
      121 gatcaaattc taaaagctgc ttatgatgtt gttgcaaagg aagggtttaa aggatttact
      181 gcgagaaata ttgctaaaaa gatggggatt tcgacacagc cgatttatct ggaattcaaa
      241 aacatggaag atttaaaaaa tacgttgctg gattctatct ttgaacaact aaaagaagat
      301 gttttcccta ttgaacgtac tggtgataaa atcattgatc tcggtttgaa ctatatctac
      361 tttgcaaaaa agcatcatag tctttacatg gcattgtaca ttgatgaaca tggggacgga
      421 aagaaaatgc atgatttttc atacaattat ttccgtgaaa tagttaaaga agatgaaaga
      481 tacaatcaat tatcggatga atatgtcgat gctctgcata ctggtacatg ggtagcagtg
      541 actggcttag cgtcattgat gtcatcaaac atcatgcatc caacagaaga acaaatcatt
      601 gcactgatca aacggacaat tgactcgatt ttagcattag ataatcctga aataatcaga
      661 tagtgttcta tcagtatgaa tttgatcggt aagtccgttg gctgcaaact gtcaatggac
      721 ttttttgcta aaaaaagcga tttctgttat ttatctatta gaaataggct tgacatttcg
      781 tgaaaaaagt ttaaaaatag tacttacgga aggttggcag agtggtaatg caccggactc
      841 gaaatccggc gaaccggcta ataccggcgc gcaggttcaa atcctgtacc ttccttatat
      901 caacgattag aggttgtgaa gaagctgtcc tgcatcaagt aataagaacg gccaaacaaa
      961 aatagctttt cctatttttt cgtttggttg ggcttattac cgcagatgca agcttttgaa
     1021 caccgtttgt tcggaataag gggctgtgac aagacttttg tcacagcccc ttattgttgt
     1081 acttttaacc tacaatattt tttgattccc cctgagtact attttttata aatgctagta
     1141 aatcaatctt ctttggcctt ttttttgata aaaaaaaata ctagatgtaa gataaaagtg
     1201 taaaaacgat ttgttttagg agggaaaacg atgtgtaccg caatcactta tgcaacgaaa
     1261 gaccattatt ttggaaggaa ttttgattat gagatgtctt ataatgaagt agtcactatt
     1321 actccaagaa attatcgttt cgattttaga aaggtaaaaa atttagataa acattatgca
     1381 atgataggaa tagcagctgg ggtatccaac tatccactct attatgaggc tacaaatgaa
     1441 aaagggttga gtatggcagg actgaatttt cctggcaatg ctgattataa agaattacaa
     1501 gaaggaaaag ataacgttgc accatttgag tttattccat ggatactagg gcaatgctca
     1561 accatagacg aagctaaaga attattggct actatcaatc tagtcaatat cgattttagt
     1621 gaaaaactac ccttgtcacc tttacactgg ttattagctg ataaagaaaa atcgatagta
     1681 attgaaagta tgaaagatgg acttcattta tatgataatc ctgtcggtgt gttgacaaat
     1741 aatccgccat ttgattatca attatttaat ctgaacaatt atcgctcgct atcaaatgga
     1801 acaccagaaa atcatttttc aaaccagatc agcttgaatg tttatagtcg tgggatggga
     1861 ggattgggct tacctggaga tctctcttct gtttctcgat ttgtaaaagc cacatttact
     1921 aaaatgaatg ctgcatcagg tgattcagaa tctgaaagta tcagtcaatt ttttcatata
     1981 ctaggctcag tcgagcaaca aaaaggagta tgcgatactg gagaaggaaa atacgaatat
     2041 acgatttatt cttcttgctg taacgttgat aaagggattt attattatcg tacgtatgaa
     2101 gacagtcaaa ttactgctgt aaatataaat aaagaagact tggatcgtca tgaattggtc
     2161 agttatccag tcataaaaga gcaacagata aactatgtca attaattaat gaatagatcc
     2221 gtttggtatc atattttctg atacagtcat aattagacac gatctttagg tcgtgtcttt
     2281 ttttgctgtt ttttttggaa atagcatcca ctaatatttt tttaacacga agagaataag
     2341 gtagcctcaa aaaagaaaac atggtaaatt agatattggg actttatttt ctgtaaaaac
     2401 agaaactttc atcttttgtt ttcacgtgac gaaattgtaa gagagaaaat attcgtccgc
     2461 tatttattct atgataagat gcatatgaat gaaataaaca gtaattttat attaggatgt
     2521 gaaagttaaa tgtgcggtat tgtaggtttt gttaacgaca aggacaacaa aaaaacaatt
     2581 attaatacaa tgatggatcg aatcgttcac cgtggaccta atagttcagg agaatatatc
     2641 gataaacatg ttgctttagg attcagaaga ttaagtatta ttgacttaga gggcggtact
     2701 cagcctattt acaatgaaga tcggacaaag atcattattt ttaatggcga aatctataat
     2761 taccagcctt tgagagaaga attgattgca gctggccacg tttttcaaac acatgcagat
     2821 acagaagtcc ttttgcatgg ttacgaagaa tggggaacag aattattgca aaagatccgc
     2881 ggaatgtttg cttttgctat ttgggataat gaaaagaatg agttattcgg tgcaagagac
     2941 cactttggga tcaaaccata ttattatgca gaaatgaacg gaacatttat gtttggttca
     3001 gaaatcaaaa gttttctgcc tcatcctgat tttaacaaag aattgaacaa agaagcgttg
     3061 aaaccttata tgactttcca atattctcca ttaaacggcg aaacattctt caaagatgtc
     3121 tatcgtttgc ctgaaggcca ttattatacg tataaagatg gaaaactaga cattcagcaa
     3181 tattgggatg cagattttga aacaaaagaa acgcattccc gacaagaatg gatcgaaaaa
     3241 atcgacgaaa ccgttcaagc ttctattgaa gcacatacgg tcagtgatgt agaagttggt
     3301 tctttcttat ccagcggggt tgattccagt tatgtaacct ctgtattgaa accagatcat
     3361 tctttctcaa tcgggtttga tgataaaacc tataatgaag cgattgaagc cagaaaacta
     3421 accgaactat tggacttaga taatacagca gcagtgatcg acggagacat gtcgttcaaa
     3481 gcattccctt tgatccagta tcacttagat gaacctgatt caaatccttc ttgcgtacct
     3541 ttgtactttt tagcaaatct tgcttcacaa agtgtgcggg tagtccaatc tggagaaggg
     3601 gcggatgaac tgtttgcggg ataccaaacg tatggattcc atacgaattc aaaattcatc
     3661 cgtgtgattg ctcaaggatt gaaaaaatta ccaaaaggaa cacgctataa tttaggacgt
     3721 aaaatcggaa aaatgaaaaa tttccatgga cggatccatt tatatgaatc acttgctcct
     3781 gcaaaagaat attttatcgg tcatgctcga gtgttcgaag aatcagaagc atcagaagtg
     3841 ttaacaccaa aatatcaaac agcaccatct gtggatgaaa tcatgactgt tcattacgag
     3901 aaaacagaag gaatcaaaga tgaagtcaat aagatgcaat atgtcgatct tcatcaatgg
     3961 atgccaaaag atatcttgtt aaaggcagat aaactttcta tggcaagttc tttggaagtc
     4021 cgagtacctt tattggatat cgaagtcatg aaattggctc aacaaatacc aagtaagttt
     4081 ttattgaatc aaaacaacac aaaagatatt ttcagacaag ctgcgaacaa acatcttccg
     4141 gaagaatggt cgaatcgtgt gaaattagga ttccctgtgc caatcaaagc ttggttgaaa
     4201 gaagaacacg gctacgaaca agtcaaagca ttattcgaag cagattttgc aaaagaattt
     4261 tttgatcaag aaaaaatcat gaagttgcta gatgaccatc acgaaggacg taaagaagag
     4321 caaagaaaaa tctggacaat cttcagtttc ttgacatggt ataaagtcta ctttgtagat
     4381 gaaacgattc cgcaagcaga agcaatcgac tatgtgactg tttgatgaaa atcaaatatc
     4441 aataaaaaag aggttttctc ctaccgttta acactgagtg ataagaaaca atttcaaaaa
     4501 acagctcttc ccgttttata gatattatga cttatggttc ggagttggca gaggaacacc
     4561 attgtttcga acataagggg gttgtgacac gattttcgtc acaacccctt tacatatgtt
     4621 caataatcga tttctttttc ttttgtccca tcttttttat aaatgaacaa agttgattct
     4681 ttgttttgtg ctacttgctt tcctttttcg attgcttctt tttttgtatc aaaatggttg
     4741 cttgcctttt ttgccccttt agatttcacg atccattgtt cttcctcata ttctacgatc
     4801 acatctgaat caagcagttt tccagcacga ggattcgtat cgtgttcatc cgtcttgctt
     4861 ggattttttt ctttttcgaa agttcgtttt tctgattcac tagcatcttc ataccatttt
     4921 tttgcttgat caatggcaat tgggatcgca cggttatcag ggtacccttc atctaataat
     4981 gcatttgcga tgtcgattgc tttttttctt gtcaatttat ctaagttttt cattgatgca
     5041 ggaaagtcct tcatgttcca tggcatatta gtatccccct ttattaaaaa acataatcta
     5101 tataaacaag tgtaagggta tttatacaaa aagaaaaaga ttttgtttga gaagtaaaac
     5161 cgttgtattt atcgtcaagt agttttactt aacaaaatcg tgaaaaacac tagcaatcta
     5221 gaaaaaatca tggtaagata atcaagtctg agagtcatct tagaggattc ttgtaaaaat
     5281 gggagggaaa aacatgcgcg taaacatcac tttagagtgt acttcttgta aagaacgtaa
     5341 ctatctaaca agcaaaaaca aacgtaacaa tcctgatcgt ttggaaaaac aaaaatattg
     5401 cccacgcgaa cgcaaagtta ctttgcaccg tgaaacaaaa taatgttttt aaagttctca
     5461 attccttgct ggtcaaagaa ttgagacttt ttttatctca aaacatctaa aatcataagg
     5521 agcggataat atgtttattt ccatgtgggc acaagataaa aatggtctga tcggcaaaaa
     5581 tggtttgtta ccatggcgat tgccaaatga tatgcgtttc tttcgcgaac atacaatgga
     5641 tcgaatttta gtgatgggaa gaaagaccta tgaaggaatg ggggatcttt cacttcctta
     5701 tcgccatatc atcgtgttga caacgcaacc aaattttcgc acgaaagaaa atgcggaagt
     5761 aatgcattcg atcgatgaac ttttgtcgta tagtcaaacg atctcagaag atatctatgt
     5821 ttcgggtggt agtcggattt ttcaagaact tcttccttac actggtcgga tctggcgaac
     5881 attgatcgat agcacgtttg aaggcgatac gtatattggg aatattgatt tttcagactt
     5941 cgcccttata gaggagtatg aggggataac ggatcaagaa aatctatatg cccatcgttt
     6001 tcaaaaatgg gaaagagtaa aaaatagggt tgcttcaaaa gaagaataaa atctatgcgt
     6061 ctagtttgtt aataagaaaa aaagcatctc acgactaatc gtgaagattc tttttttagt
     6121 taaaatcaat ttagtttttc tttgatatca ttgaccattt cctgactggc aataggtccg
     6181 ttatatagcc aactagattc aggtccgaat tgcagtacat gattgtctgt cactgcttta
     6241 agattcttcc agcttggttc attgaacatg gcagcatctt tgtcactgtt cacaaggatg
     6301 atgtagtcag catctaattc agctagcttt tcagaggaaa cagcagacca gtcagaagtg
     6361 gcttttttgg agacctcttc cactagatca ggaacaccaa attccagatc gtcgtatacg
     6421 attcttccac ttgaccgatt tttgctgacc ataaaggctg agttgttggt cacccacaac
     6481 actgcagctg tcttgttctc gattttatct gctagttctt ctcgagtttt cttgatggat
     6541 ttttgataat cggaaatgat tttttctgcc tctttttctt ttgccaaagc tttgccgaca
     6601 tcttttagtt gttcttccca tgtgacatct gttccgtttt ttacaacata agttggcgcg
     6661 attttgctat attgttcgta tttcccgcct tcgacagtag cggaagaaga aatcaacagc
     6721 aaatcaggct caaagcttaa cactttctca taaggaaggt cataagaaat cgttgggaca
     6781 tcctttaaat catcttgcag ataatcttga atggaaccac tgccaacagt ccactgggcg
     6841 ataggttttt cccctagtgc aatcagatag tcttctaagt aactgccgat gatccgtttg
     6901 ggcgcgtttg gaatctctac tttatgtcca agtgtatctg tgactgtatg agtactattg
     6961 gatgaagtcg tcgaattggc agttgtagct gtttctttat ctgaagaggg ggagcaagcg
     7021 ctcagtactc cagccagcag caatatgcta gataatgtga atacttttgt ttttaatttc
     7081 atttctttcc tcctaacata attgagaatc tttctcattg gttattattc ctgatatttc
     7141 gaacgctgtc aaccgcttca tttggctata gatatgaaag tagcgcttga cgaaatgttg
     7201 aaacagcgtc ataattgaga atgattttca gcgaaaggat gggtgaaatg acgagattaa
     7261 gagcagctga cattgcagcc ggctacgaaa acaaaaaaat cattgaaaac atgtctgtta
     7321 cgattcctga agggaaaatc actagtttga ttgggccaaa tggaagtgga aaatctactt
     7381 tattgaaaag ttttacccgt atactacctg tgaaatcagg aaagatcatt gtcaataact
     7441 ctgatatctc tacatacagt tcaaaagaac ttgcaaaaaa aattacatta ttagcacaaa
     7501 ctagtgaaca ccctacaggt atgacagtag aagaagtagt atcttacgga cgataccctt
     7561 atcaaaaact gttcagtggc atggacacgc aagatcatga agcaatcgag tgggcgttgg
     7621 atgctacagg tctagctgga tttcgtatgc gggatatcgc aacgctgtct ggtggacaaa
     7681 cccagcgtgt ctggatcgct atggcgcttg ctcaggaaac agatatcttg atcttggatg
     7741 aaccgacaac atttttagat ccggcacacc aattagagat cctacagtta ttgactgaaa
     7801 tcaatcaaat gaaacaaaca acgatcttga tgtcgattca tgacattaat catgcttcac
     7861 gtttttcaga ttatttatta gcaatgaaaa acggtaagtt gatcacagaa ggtacacctg
     7921 aagaagtgat cacaaaagag tgtatgaaaa aaatatttga gattgatgca acgattgtca
     7981 cgcttccaag aacaaataaa ccagtttttc taacctatga tttgttgcca aaagagcagg
     8041 aggatcaatt atgaggacta tgaagaaaaa tagactcctg cttttggtct gttttgttct
     8101 tttgattatt ttttttgttg ctctttcaaa tggcgcagcg actgtgggaa aagcagattt
     8161 ttggcaggcg ttgtttgatt ttgatagatc aaaacaaagt caccaaatca tacgaaatat
     8221 tcgtttgcct cgtgtggcgg gagcatttct tgtagggagt tgttttgcat tgagcggtgc
     8281 attgatgcaa ggagtgacaa gaaatccttt agcagattca ggcttactag gaatcaacgc
     8341 gggtgcttcg ttggcaatgg cccttagttt tgctttttgg cctaaagctt ctgatttttc
     8401 tgtgtttcta atgtcattgg caggttcttt tgctgtaact ttgctcgtat ttggtttttt
     8461 gcagttttcg aaaatgggca tgaatccttt acagctgatt ttagctggcg tagctattag
     8521 ttcttttttt actgctatca gccaagcact tacccaattc tttaatctgc agcaggatct
     8581 tgctttttgg tttgtaggcg gagctgccaa tatcacatgg aaacaattag cagtattgtt
     8641 tcctttatat atacttgctg ttttggtttc tttttcatta ggaaaagcaa tatctttgat
     8701 tcatttgagt gaaacacatg cattagctct tggaagtcat cctaaacgaa cgcggttgat
     8761 tgttttctgt ctagttgcag tgttagcagc cattgccgtt tcgttagtcg gacctgtttc
     8821 atttattggg ctgttggttc cgccagttgt tcgcggaatg gttggaacgg actatcgtta
     8881 tgttctacct gtctcttttt tagttggcgg tggattggtt atgttggcgg attttacagc
     8941 gaggatgatc aatccccctt ttgaaacacc ttttggatta gtcattgcat tgattggcgt
     9001 tccgttttta ctgatacaag taaggaggga acaacggtga aaaaaatata ttggacgttc
     9061 tatttcttgc tttttgtact tgtcatcctg tctataggta gtttaatgat tggtacgcct
     9121 tttatttacc tggatcagct ttttgccacg ttcataggga aagaatcatc ttctttggcg
     9181 ctgattgtta gagaatttcg agttcctcga ttactgatca gtttattagc aggagcttgt
     9241 cttggcgttt ctggttatat cttgcaagga gtaacaagaa atgaattggc agattctagt
     9301 atattgggca ttaatgccgg agcaggattt ttagtgatgc tttatttagg tttcttttcg
     9361 cactattcgc tgccgtttct attgctagtt gtagcctttc taggtggtgt tttagctgct
     9421 tgctgtgtct atcttgcggc ctatgatcga aatggctttt tgggtatgaa tcgaatctta
     9481 ttgtccggta ttgccgttaa tgccggcctt tctgcattaa cattgctatg cacgatccaa
     9541 ttatctaagg aaaattatgg atttgtgaat gcatggttag cgggatcaat ctggggtgcg
     9601 agctggcctt atgtttgggc attgcttcca tgggcggtta tcttaattcc gctaggagga
     9661 ctttatgcac agaggattgg attgctatca tttggtcaag aacgcgcgca aggattaggc
     9721 cttaacgtgg ctaaaagtca aaaaatcctt ttgttgttag ctgttgcttt ggcaagcggt
     9781 agtgtagcgg tgacaggtag tttatctttt gtaggactac tagctcctca tgctgcgaag
     9841 ctagtcgtac gtaaagaaaa taactggacc tttgttctga gcggtttgtt cggttccatt
     9901 tttctgctta gtgcagatat ggttgctaga gtgattctac caagtgggga agtacctaca
     9961 ggaattttga ttgccctttt tggtgcgcca tattttctgt atgcactttt tatcagacaa
    10021 aaacacgcgc tgaacagttg aaatttatta aaaaaggaag gccaatcatt tctaggtctt
    10081 ctttttattc tatttatgca tttatttgaa agttcgattg ctatttttct ttttttccta
    10141 taccatttta tgagagaagg aagggtgagt ttttggacca attgaaagta agattacgcc
    10201 ggaaaggaat cgaaaagctg aaaaatctgg ccaaaagtgg caagaaaaag gaaaaagaac
    10261 agcgaatcta taaacagcta ttcgccagcg agtactggaa gtcagcaaaa gtgataggga
    10321 cgactttatc gaatgaattt gaactggata cacagccggt tatccaacga gcagaaaaag
    10381 aacaaaagaa aatagttgta cctaaaactt tgccgaaacg tcagatggcg ttttatgaaa
    10441 tggatgaaca gtccgttctt gtacgttcga gttttggtgt tcttgagcca caatcagaga
    10501 agctctattt gccggagatg atcgatctcc tcattgtacc aggtattatc tacaatagag
    10561 ccggttatcg aatcggtttt ggcggtggtt attacgaccg gtatttaaat aattttcata
    10621 aaaaaacatg tagccttgtt tttcgtgaac aattgaatga acaatggcaa ccggagagct
    10681 ttgatcagaa gatccaacaa ttgtttattg actaaaatga aatgggaggt tttcatgaat
    10741 tatcaacagc aaataaaatt gcgtgcatgg ttaagacgac cgttatggac ctatgctttt
    10801 ttagggatac agacagcggt cttcctcatg atggaattat ttccgcgact tgagatccct
    10861 tattatgccg gaatgtatgg tccttatctt gttcacttca atgaatggtg gcgtttagtg
    10921 acaccaatct tcatccattt tggactgatg catttcgtaa tgaactcact gatactgtat
    10981 tttatggggc agcaaatcga ggcaatctat ggccattggc gttttttctt gatttatatg
    11041 ttcagcggga taatgggaaa tacagcaagc tttgctttta acgaagcgaa tgttctgtct
    11101 ggtggtgcga gtacttcgat attcggtttg ttcggtgcgt tgtttatctt agggtttcat
    11161 tttaaacata acccagcgat ccagcagcta gtcagacatt ttcttttatt tattgtgttg
    11221 acatttgttt ttggtcttct ggatacctct gtagatgttt ggggacatgt cggtgggcta
    11281 attggcggat tagtgttagg gaatgttttg ggtctaccga aacaacagac aagctattcg
    11341 atacaccaaa ggattctttc tacactagtt tttgtatttc tttttgtaat atgtatttta
    11401 ctgggtttaa aaaaatatgg tttacttgta taatatgaag gaagtgctta aagatggaga
    11461 gcttatatga tgtacagcag ctgttcaaaa aatttggcat ctttatctat gtgggtgcac
    11521 gcatctatga cattgaatta atgactatcg aattgcgtaa tctgtatgat agccgtttga
    11581 ttgatcatga tacatacatg acagcgtggc gcatattaaa aagagagcac cgaattgaag
    11641 aaagccgaaa gaaaggaaat ttgtaatgga caagaagatt attgggattg atttaggtgg
    11701 aacaacagca aaatttgcaa ttttaacacc agaaggagag attcaacaaa aatggagcat
    11761 cgataccaat attttggatg atggtaagca tatcgttcct gaaatcattg aatcaatcaa
    11821 ccatcgtttg aatttatata atatgaaagc cgaagacttt atcggaatcg gcatgggtac
    11881 cccaggaagc gtggacagcg aagctggaac ggttattggt gcgtataact taaactggac
    11941 agaagttcaa tttgtgaaaa aactgatcga ggcaggaaca ggaatcaaat tcgctatcga
    12001 taatgatgcg aatgttgctg ctttaggtga acgatggaaa ggtgctggag aaaacgatcc
    12061 agacgttgta ttcgttactc taggaacagg tgttggcggt ggtatcgttg ctggcggaaa
    12121 tttgattcac ggcgtgagcg gagcaggcgg agagatcggc catatcactg tggatcctga
    12181 agggttcgaa tgtacttgcg gtaaacgcgg atgtttagaa actgtgtcta gcgctactgg
    12241 tgtggtacgt ctaggtcgtt acttggcaga agaatacgca ggagattcaa aactgaaagc
    12301 aatgttagac aacggtgaag aagtaacaag taaagatatc tttgaaatgg ctcaagaaga
    12361 cgatccgttt gcattgatgg ttgttgatcg cgtatgcttc tacttaggtt tagcttgcgc
    12421 caacttgggg aatacattga atccgtcaag tattgtgtta ggtggaggag tatcggcagc
    12481 aggagaattt ttacgtagcc gtgtagagaa atattttaaa gaatttactt tcccgcaagt
    12541 aagagaatca acaaaaatca aattagcaga gcttggtaat gaagctggtg tcattggtgc
    12601 tgcctcatta gccttgaaat ttgcttaaaa aaacggaggc attacatggt tttatgggta
    12661 attaatatca ttttgctatt gattgtgctt gcaatcatct tttgggaagt ctatctacga
    12721 gtaatggcca agcgttcagc aacgactttg acagaagaag aatttcgaga aggaatgaga
    12781 aaagcacaag taatcgatgt tcgcgaaaaa gatgtgtttg atgctgggca tattttaggt
    12841 gcaagaaaca tcccttacac tgttttgaaa gattcattag gatctattcg aaaagaccag
    12901 cctgtctata tttacgatca aaagaaaagc ttgagcatcc gtacagcaaa taaattacgt
    12961 aaagcaggtt atcaagagat ttatttactt aagggtggat atgacggatg gtctggaaaa
    13021 atcaaaagta aaaaagtata aaacagatga ttgtatgaca gctttaaaaa ccgaatcaat
    13081 cattagattt tgaccaagat cacatgaacg gcgcaatgga tagttgtgcc gttcattttt
    13141 tttattttgt gctattatct acatatataa gctaagcgaa aaggcagaaa gaaggaaaca
    13201 actatgatca aattaaaacg tgtttacctt gatgctgaaa aagaggatgg ctatagaata
    13261 ctcgtggata ggttctggcc acgaggaatt tcaaaagaaa aagcgcagtt agatctgtgg
    13321 ttgaaagaaa ttgcacctag taatgaagca cgcaaaactt ttgaccatgt accagaacga
    13381 tttgcttcat ttgcaaagaa ataccagcaa gaattgattt tcgaagaaga aaagaagcaa
    13441 gctattttta aactcagtca acttatgaaa gaacatccag tcattacttt tgtttatggt
    13501 gcaaaaaatg aacatcagaa ccatgctgtt ttattgaaaa aatttatcga aatggaggca
    13561 tgcaaatgaa aatagggatc attggtgccg gacctagagg attaagtata gcagaaagga
    13621 ttctccataa taatcaggac agacattctc ttgaactttt tcttttcgat cccaatggtc
    13681 ctggaggtcg agtttggcgg ttgaatcagc caccggagtt attaatgaat tctgtttctc
    13741 aacaagtgac gctttttaca gatgaaacat tgtccagtgg aggattggta acacagggac
    13801 ccaacttgtt tcaatggagt aaaggacctg ctacagatta tatcgaacag acggtaacca
    13861 aaaataagca actaattata gaagaaatca atcgtctgga aaaagatcag caatctactc
    13921 gatgtttata cggactctat caacgctggt ttttctcaaa gttgcaagaa gaatttccag
    13981 cttctattca gtttgtttat tctttagtaa aaaaagtcag caaaagaaaa gatggttatc
    14041 ttttacagac agatcagcaa agctattcta tcgatcagct tgttttagca agcggtcact
    14101 gggaaaatga tgagacagaa gaagaaagag tgtgggtaga ctacgcaaag aaaaatgatt
    14161 tactgtatca gcttccttct aatcctgcag atgtcgatct ttcgaagata ccgcctaaag
    14221 aaaatgtctt tttaagaggt ctaggcttaa gtttttttga ttatgtcgga ttgtttacag
    14281 tatcaagaga agggcgcttc gagaacgaaa aaggaaaact tgtctatcat ccatcaggca
    14341 aggaaccgat tgtatacagt ggatcaagaa gggggctccc ttattaccct cgaggaagaa
    14401 accaaaagca aggcggatca atggcatggc cacggttact aacaaaagaa aacctggagc
    14461 aatggcatca gtcgggattt ttgactggcg aaacattttt tgactatctg aaaaaagacg
    14521 ttgagttgtt ttattacaag aaaatcatcg aagaatatca tttgccgatt ccaaagaaaa
    14581 attttgagca tgattttctc tccctctctt cagaggagtg tctcggaaag catccagcac
    14641 taatccctta tagatgggat tggtcgtatt tagaaattcc tcttacctat caggatgagt
    14701 cctttgcaga agctagccga gcatttattg aagggcagat aaaagaagcg gaaaaaggca
    14761 attgcacagg agcatttgct tcaacatttg atgcattaaa agactggcgt gatccaatcc
    14821 ggcaggctat tgaatggcac gttttttctg caaaggaata caaagaaata ctatggggat
    14881 ggtttactca tctaaatagc tttttaacga tcggtccgcc agtcattcgg acgaaagaat
    14941 tagcagcact gatagatgcg ggtatttttc atttgttcga accgccattt gatattcagc
    15001 aaaagcacaa ccgctatgtt atttctctac aagataaaga aatcagcagt cgctatttga
    15061 ttgaagctcg attgccacaa acaagctttt ctcgttcaag taatcctgtc ataaaaaatt
    15121 tgaaggaaaa caaacttgtt cgcccttttt cattttcaga taatacagga agttatcatt
    15181 cagaagctat agatgttgag cgaggtacga atcgcgtgat tgatgcttca ggggaaatcc
    15241 agtctgactt ttattgctat ggaatacctg ttgaaggtgt ggattggcta actgcagcag
    15301 tagctagacc ttatacagat ccatggaatc tgagacaggc agataaaata gcacaactta
    15361 tattgactga caaataaaaa tacgcatgat gacttatctg ttctttcaca agtcttcatg
    15421 cgtgttttta aataattatt aacgagaaac gcgtttgcgc attgcttttt tacggttttc
    15481 ttcgatcatt gcttcttttt cgtcaattgg atcgatcact gtttttttca cagaagcgac
    15541 caaaccagca acggcagcgg ctgttgccag acttcctact aatattccag atacaaattt
    15601 tttcattgaa tccacctcat ttattatttg ttatatctat tatggaacaa aagagaaaaa
    15661 aaggctagtt ttacgtttgt ttgtaaacct ttgcaaaaaa tccttgaaaa tgtttactaa
    15721 atgttttatt ataaatttac catatgaatt tacgaaattt tactttgttt gttttcacca
    15781 taaaagtaaa cttcgttttt ttaaattata tataatcaac aaacacaatt ttactaatca
    15841 aaggagtgtt ttttatgaca attttagtac taggcggagc cggttatatc ggttcgcacg
    15901 ctgtagacca gctagttcaa aaggggtatc aagtagctgt ggtagataat ttgctcacag
    15961 gtcacaaaca agcagtacat ccagatgctc atttttatga aggagacatc cgggataaag
    16021 aatttctacg ttccgtattt gaaaaggaac cgattgaagg agtaatccat tttgctgcaa
    16081 gctctttagt aggagaatct gtcgaaaaac cattgatgta tttcaacaac aacgtatacg
    16141 gtatgcaaat cttacttgaa gtcatgcacg agttcaatgt gaataaaatc gtgttctctt
    16201 ctactgcagc aacttacgga gaaccaaaag agtctccaat cacagaagat acaccagcta
    16261 atcctaaaaa tccgtatggt gaaagtaaat tgatgatgga aaaaatgatg aagtggtgtg
    16321 accaagcata tggcatgcgc tatgtcgctt tgcgctactt caatgtagcc ggagcaaaag
    16381 cagatgcatc tatcggagaa gatcatacac cagaaacgca tcttgtccca atcattttac
    16441 aagtagctct tggtcaaaga aaagcattgg ctgtttatgg agacgattat gatacaccag
    16501 atggaacatg tatcagagat tatgttcaag tagaagattt gattgctgca catattttag
    16561 ctttggaata cttgaaagaa ggaaatgaaa gcaacttctt caatttaggc agcagcaaag
    16621 gatactcagt caaggaaatg ctagaagcag ctagagaagt gacaggaaaa gaaattccag
    16681 cagaaatagc accaagaaga gctggagatc ctagccgatt agtcgcttca agtgaaaaag
    16741 cgagagaggt tctgggttgg aaacctgaat acacagatat caaagcaatt atcaaaacag
    16801 catgggattg gcatgtcagc catccaaatg gttatgaaga ataggggaat gagatatgtc
    16861 aatcagtcaa acgatttgtg attttgccac acttgcgatc gaagctggcg gatggatgga
    16921 aatggaccgc ttatatttac agaatcgtct tttagggatg gtaggagaag atgctctaga
    16981 aagcattgaa gtaagaccag ttaaggaaga atctgtagac ttattggatc agcttgtcgc
    17041 taaggcaaga gaaaatggtg ttctttcaga attgccagca gaacaagaga tttttgaagc
    17101 gcaattaatg gattttctca caccgccacc ttcagtcgtc aatgcattct ttgcacagca
    17161 ctattcgaaa agcccagagg aagcaactga gtatttcttt aatttaagta agaaaaatga
    17221 ctacatcaaa acaagagcca tcgcaaaaaa catcaaattt ccggcagagt cggagtatgg
    17281 tgaactggaa atcacaatca atttatctaa gccagaaaaa gatccaaaac aaatcgcagc
    17341 agaacgcgag gcggtaaaag tagactatcc tacctgcatg ctttgcatgg aaaacgaagg
    17401 atatagaggt cgcttgaact atccagcccg caccaatcac cggattattc gaatgaatct
    17461 agacggtgaa agctggggat tccaatattc tccttatgct tattacaatg aacattcgat
    17521 cattttatca gaacaacacc gcccgatgaa aatcacaaag gaaacatttg ctagactgct
    17581 tcggattact gaagtcttgc ctcattactt tgttggttcg aatgctgact tgccgattgt
    17641 tggaggatcg attctttctc atgatcatta tcaggcagga cgtcatgaat ttcctatggc
    17701 aaaagcaccg atagaaaaac ttatcaaact agcagattat ccgcaagtca tcgcaggagt
    17761 cgtgaaatgg ccgatgtctg tgatccgttt gcaatcaaca gagaaaaaat tgttgattga
    17821 agcagctaac tcagtattgg aaaagtggaa agcatattcg gatgaaacag tatctatcag
    17881 agctttttca gcagatggta caccacacca tacgattacg ccaattgcac gaagaaggga
    17941 caattacttt gagttggacc ttgtccttcg agacaacaat gtttcagaag aacatcctga
    18001 tgggattttt catccacata aagatgtcca acatatcaaa aaggagaaca ttgggttgat
    18061 cgaagtaatg gggcttgctg ttttaccacc tcgtttgaaa ccagaactag tagaagtaga
    18121 gcatttctta ttaggagaag aaaaccagat ggctgaatat catcgtccgt gggcagaaca
    18181 attaaaaaaa gaacatcctg atttgacgaa agatacggtc accgatgtcg tacaaaaatc
    18241 tgtaggtcag gtttttgcac gtgtactgga agacgctgga gtattcaagc gagatgaaaa
    18301 aggacaggca gcgtttgaac gatttactgc aattttataa acgtaaaaaa ggaggagaga
    18361 caaatggcaa caattaaaga cattgcgcag ttggcaggag tttctcctgc tactgtttca
    18421 agagttctca actatgatcc tgatctttcc gttggcttag ccacaaagca aaaaattttt
    18481 gaagcagcag aacgcttaaa ttatacgaaa catcgtaaaa atgcagcaaa tactaagcaa
    18541 acgcttcgcc tgatccagtg gtacgacgat acggaagagc ttgaagatct ttattattta
    18601 tctattcgtc taggaatcga aaaaaaggca gaagaagcaa acgttacttt attgaaagaa
    18661 acgtgggatt ccattagtga tcttccgaca gatggaacga tcgcactagg aaaattcgac
    18721 gaagctcagt tattagaaat gaaaggatcc caaaatgcct tgcttttggt agattctgac
    18781 ggcacttctc aaggaatcga ctctctagtt gtcgacttta agtcaagtgt tcaaaaagtc
    18841 attgaacatt ttgtctccca taaagcgcaa catatcgcta tgcttgctgg aacagaatat
    18901 acgaagaaaa accatcagcg gctaaaagat cccagaatta tggcatttca acaagttttg
    18961 gcagaaaaag gctacgacga aggtccaatt atcgaagcag atttttctgt cgagtcagga
    19021 tatcaagcag tgaagcagta tttaaaaacg aatccgcatg ttcctgatgc gctttttgca
    19081 gctaatgatg cattagcgat tggcgcactg aaagcgattc aagaaaaagg tttagtggta
    19141 ccagaagata tttctgtgat gggtttcaat gatgtcagtg tagcgaaata tgtaacgccc
    19201 tctctttcta ctgtaaaagt ttataccgaa tggatgggag agctagcagt ggagacgatt
    19261 cttcaattaa gtaattccaa agcaccggtt tcaagaaaga ttattgtgga aacagagttg
    19321 attattcgag aatcaacgaa ataaa
//
LOCUS       DAZBSA010000051        19294 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000051 DAZBSA010000000
VERSION     DAZBSA010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.051"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..517)
                     /locus_tag="ACHGMT_001653"
     CDS             complement(<1..517)
                     /locus_tag="ACHGMT_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835395.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETP"
     gene            784..2484
                     /locus_tag="ACHGMT_001654"
     CDS             784..2484
                     /locus_tag="ACHGMT_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFN6835396.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGRGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            2454..2672
                     /locus_tag="ACHGMT_001655"
     CDS             2454..2672
                     /locus_tag="ACHGMT_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFN6835397.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            2692..3582
                     /locus_tag="ACHGMT_001656"
     CDS             2692..3582
                     /locus_tag="ACHGMT_001656"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFN6835398.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            3600..3884
                     /locus_tag="ACHGMT_001657"
     CDS             3600..3884
                     /locus_tag="ACHGMT_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835399.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            4371..4985
                     /gene="gmk"
                     /locus_tag="ACHGMT_001658"
     CDS             4371..4985
                     /gene="gmk"
                     /locus_tag="ACHGMT_001658"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFN6835400.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            4990..5295
                     /gene="rpoZ"
                     /locus_tag="ACHGMT_001659"
     CDS             4990..5295
                     /gene="rpoZ"
                     /locus_tag="ACHGMT_001659"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFN6835401.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            5548..7956
                     /gene="priA"
                     /locus_tag="ACHGMT_001660"
     CDS             5548..7956
                     /gene="priA"
                     /locus_tag="ACHGMT_001660"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFN6835402.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            7971..8462
                     /gene="def"
                     /locus_tag="ACHGMT_001661"
     CDS             7971..8462
                     /gene="def"
                     /locus_tag="ACHGMT_001661"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFN6835403.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            8455..9393
                     /gene="fmt"
                     /locus_tag="ACHGMT_001662"
     CDS             8455..9393
                     /gene="fmt"
                     /locus_tag="ACHGMT_001662"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFN6835404.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            9393..10751
                     /gene="rsmB"
                     /locus_tag="ACHGMT_001663"
     CDS             9393..10751
                     /gene="rsmB"
                     /locus_tag="ACHGMT_001663"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFN6835405.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            10764..11504
                     /locus_tag="ACHGMT_001664"
     CDS             10764..11504
                     /locus_tag="ACHGMT_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFN6835406.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            11501..13570
                     /gene="pknB"
                     /locus_tag="ACHGMT_001665"
     CDS             11501..13570
                     /gene="pknB"
                     /locus_tag="ACHGMT_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFN6835407.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            13735..14634
                     /gene="rsgA"
                     /locus_tag="ACHGMT_001666"
     CDS             13735..14634
                     /gene="rsgA"
                     /locus_tag="ACHGMT_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFN6835408.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            14647..15297
                     /gene="rpe"
                     /locus_tag="ACHGMT_001667"
     CDS             14647..15297
                     /gene="rpe"
                     /locus_tag="ACHGMT_001667"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFN6835409.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            15298..15936
                     /locus_tag="ACHGMT_001668"
     CDS             15298..15936
                     /locus_tag="ACHGMT_001668"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFN6835410.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            16091..16945
                     /gene="mreC"
                     /locus_tag="ACHGMT_001669"
     CDS             16091..16945
                     /gene="mreC"
                     /locus_tag="ACHGMT_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFN6835411.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            16949..17458
                     /gene="mreD"
                     /locus_tag="ACHGMT_001670"
     CDS             16949..17458
                     /gene="mreD"
                     /locus_tag="ACHGMT_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFN6835412.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            17713..19287
                     /locus_tag="ACHGMT_001671"
     CDS             17713..19287
                     /locus_tag="ACHGMT_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFN6835413.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT         6533 a         3527 c         4129 g         5105 t
ORIGIN      
        1 caggtgtttc ttttttgaaa aaagattttt ttacattgat cttatctgac aagatcgaac
       61 ttaatttact agcaaaaatc ctataaaatt catctaaata gcgataataa atccgattca
      121 gctgatcatt aagatcgaag tatacaaaat tattctgtag tttataaaaa aacggcgaat
      181 gaagatgtgt tttcatatga ccaaaatata ataattctga aatttctaaa ggtgtcaatt
      241 ctttcagcat cgttaaatca gtaaagtcga tccacttcaa ttcatcgaat ccacgcctgt
      301 tacgagaaga ggaaaagaaa cgctgaatat tttctgtacc acggattact ttcagccctg
      361 tatgggattc atattctcca gcagaagcag atggatcaag caagagcaag ttttgaggaa
      421 aatgcacgat actgtttgca aagtcagtag cagtcagccc ttttgttaaa acagcattac
      481 ttgtggtatc aatatgtaca taaactgact cagtcatcat gttcctcctc ttatttcctc
      541 ttaatctctt ttattttaca acatttgcat taaaaaagct tgtttctttt attttaaaat
      601 tccattacaa aatcacgttt tcattgaaaa cactctcatt ttcaattaat ttttgtgatt
      661 tttgtatttt tttatttaaa ggaggtttta tgaattgttt tcttatggaa tctacaaagt
      721 cgaatacgag actcatgacc tttttatgac gtactggtat acttgaagaa aaaggagagc
      781 gcgatggaga taaaaaaagt ttacttttca cagaaaatgt cgctgtattc gcctatccag
      841 caagcaaaac aaccccttcc attttttcaa agtcatcaag aaagtagaga cgctttttgg
      901 cgttctgcaa aaaagcaatg tgcttttaaa gcagtatcga aaaaatatac cgtctatttt
      961 actattgcta aaagtagagg agatgttatt tttaataatc tgttgattga aggtgaaact
     1021 tttgaatgga aaaaattttt tcggtatatg gaagcttgcg ctcgtttttt tattaaagaa
     1081 aactgttgtt ttttatttca atctcctcta tcagaagaat ggttgcgtat ttttcataaa
     1141 aacggctatc aaggaaccac acaattaaat aaaaagctgg tctatcatac agcacttgtt
     1201 ttaggtagag gaggagcgca cggtgcttat cagattggtg tgtggcaggc gctgaaggaa
     1261 catgatatca actttgagat tatcacaggt acctcggtag gtgcgttgaa cggcgcattg
     1321 attttacaag gagatatgaa aaaagctgtg aatttatgga aaaagctgtc gacaagacaa
     1381 gtgcttgcgt taccagaaat ggctgcagtt gaagaccttc gtgaacgctt ctatcgagaa
     1441 agacgccaaa tgacgaaaac agcactaatt gaaggcgggg tttcttctgc tccattggaa
     1501 aagatggtca aagacaattt ggatttgtcc ttactcaaac ataatcccaa aatacgttta
     1561 tatacggtgt ctactaaact gccagaacta gcggaagtag tgactgatat ccaacagaca
     1621 aaaacagaag aaataccaga ttggatactc gcctctgctt cattttatcc cgctatggct
     1681 tatcgaaaga tcggaaaaaa taaatatgca gacggtgggt atcgcaacaa aatacctatt
     1741 gatattgcga ttaacaaagg agcaacagag gcatttgtcg tagatgtgca agggccaggc
     1801 ccagcgaaaa aaattcgtat gccagacatt tttatccatt ggaaatgtca aacattgtgg
     1861 acactaggca gctttctgct ttttgatagc caaaggaacc aattaaattt acaattaggc
     1921 tatctggaaa tgaaaaaacg attaggctat tactatggga actggtatac atttcattct
     1981 gttaaacctg caagaacata ctggcgagga tttttgtctt acttaacaaa agaaattcag
     2041 ttagaattat cttttttcaa aagcataaaa ttttggcaaa aactgcgtaa tttatataag
     2101 aacagggtcg taccagagac atgcggattg gcgatgctag aacttttagc aaaaaaacat
     2161 tttttactgc ccaatgaaat ctatcaagta gacatgatga ttcaaatgat ctgccaacaa
     2221 gatataaaaa gtatgccgga tgatttattg gcacaaattg gtcaattaag tactgaagaa
     2281 tggcgaaggt atcgaaaata ccagcaaaaa atacaaaatg agcgcacgaa aactgtttat
     2341 tttgattatc tgctacaagg aaaacgcaag gatcgtttgc agcatgaatt gcttaagcaa
     2401 ccagttgaca cattactgat cctatattta tattatctca aggaggaaca accatggcac
     2461 aagaattttc atatgaaatc gtagaagaaa tcgctatatt gtcagaaaac aacaaggggt
     2521 ggcgtaaaga actgaaccta gtcagctgga atggtcgccc gccaaaattc gatcttcgag
     2581 attgggcgcc agaccatgaa aaaatgggga aaggcctgac attgacgaac gaagagtttg
     2641 aacaactgca aaaagcaata gaaaatatgt aatgaggaag gaccctgtcg aatgaaaagt
     2701 atgactggtt ttggcaaagc catccgcgaa acaaaagcgt atcagttaga tattgaaata
     2761 aaaagtgtca accagcgttt tctagatgtt caaatacgaa gtcctaaaat cctgaacttt
     2821 gttgaaaacg attttcgtca gcttattaaa cagcagttaa gccgcggtcg aatcgaagta
     2881 tttgtgaatc tagcttatat tggcaaaaac gaaaaacaga tcgtcatcaa ctgggaattg
     2941 attgattcac ttatcactca attgacagac ggaattcaac aacgatacgg tagtcaaaat
     3001 caagtgtcaa ttagtcggat ggcggaacgc ttcgtgctgg acgaaaactt cgtaacggta
     3061 gaagaaaaac cggaagagag catggaagaa ttggcacagt tagctctaga tacattgaaa
     3121 agcgcacttg attctattga ggcgagtcga gaaaaagaag gtcaagcttt agctttagtg
     3181 ttgaaaaaga atactgaaga attcaaagaa gtactaaaaa aactcgatag ttttgtagaa
     3241 atctatgaaa aggaatttca aataaggtat caaaaaaagc tggaggaatt tcttggagcg
     3301 aaagtcgatg agcaacggtt gttgacggag ctagccatct tgttggaacg cggagatatt
     3361 cacgaggaac tggatcgtct ggcgatccat gtacaaaaac tagatgaact tatcgaagca
     3421 caatgtcctg taggtcggga attagatttc ctgatccaag aaatgaatcg ggaaatcaac
     3481 acgatcggtt ccaaatcaaa tgcaatcgaa attaaaaatc aagtcgtcca gtctaagacg
     3541 atcttagaaa aaatcagaga acaagtacaa aatattgaat gacgaaagag gtagagataa
     3601 tgaaatatta tgacgtaacg ttccacgaat taagtggaaa aactgtggtt aaacgggata
     3661 tcccttctga aaaaaacggc tttgacgtat ggaaagatgc ctgtgcagac tataatgaga
     3721 acgaactgtt tattttgatc aacgacggtg cttacgtgac gatgaatcgg aaatttattg
     3781 ttcgaattga cacagaagag gtagaagatc cgacagaaaa agcacgttct cgaaaagatg
     3841 agatcatggg tgtagtgaat actttgtcca atatgggctt ttagcaagga tgctgaacaa
     3901 agaaatacaa taaataaaca gaattggatt tcttcgctta gcttctttgc tctaggaata
     3961 catttaaagg ttgtgacaaa attcgtgtca caatcttttt ttattttgaa taagtgatgt
     4021 tcaaaaacca ttttctcggt attaagccaa atttatgcaa aaatgtgaga aactattttc
     4081 acaaaatttt tcttcatact cgaaactaat cggcttcttc acgacctctt atacacggcg
     4141 ttcaaaagct tgcatctgcg ataataaccc caaccaaacg aaaaacatga gaagctgttt
     4201 atgtttggct gttcttatta cttgatgcag gacagctttt ttacgacctc ttggtacagg
     4261 gtagcttttt taccaatttc tagctataat taaagataac cttgaaagtt gcagaaaaaa
     4321 agtgcaaaat aaacgataag tacaaaaaga ctagttgaaa ggacgtcgtc atgtcagagc
     4381 gtggattatt aatcgtgttg tctggtccat ccggtgtagg aaaaggcaca gtacgaaaag
     4441 caatttttga gagtgaggat aatgattttc agtattcgat ctcaatgact acccgtaaaa
     4501 tgcgtgaagg ggaggtcgaa ggcgttgatt atttcttccg aagcaaagaa gagtttgaat
     4561 caatgatcga agctggagag atgctggaat acgcagaata tgtcgggaat tattatggaa
     4621 ctcctttgtc atatgtcaac aagacattgg atgaaggaaa agatgttttc ttggagattg
     4681 aagtccaagg agcaaaacaa gtcaaagaaa aagtgcccga tggtgtattc atctttttga
     4741 ctccgccaga tttagcggaa ttaaaatcac ggatcgtagg ccgaggaaca gatgcagatg
     4801 aagtaatcga aaaacgaatg aaagttgcaa aagaagaaat cgaaatgatg gctctttacg
     4861 actatgctgt tgtaaatgat gaagtgccgc tagcagttga acgaatcaaa aacattattg
     4921 ccagtgaaca ttttcgagta gatcgcgtaa tcggtaaata tattaaaatg ttaaaggaga
     4981 tgtagtctta tgatgttaaa accttcaatt gactcactat tagatcgtgt aaattcaaaa
     5041 tattctctag tgattttagc aagtaaacgc gcgcatgaat tggatgcaag tgcacagcct
     5101 actctagatt cttttgattc agtaaaaagc gtcggtcaag ctttagaaga aatcgatgca
     5161 ggaaatgtag tcaatgatcc acatcctgaa ttaaaacgtg aacgtttgaa gatggaagaa
     5221 gaggaacgtc aagcacaaaa agagcgtgaa caacacgaat tggaatcacg tatccgtgaa
     5281 gagcaaaaaa tgtaataaag aaaaatagag cgaacatcta aaaagaaaag caaacccagc
     5341 tgtttccttt tcaaacattc gccacttatt gtctggtaga aaaggctgtg tctgaacttt
     5401 ttgacacagt tctttttttt cttatctgct gttaattttt tgttaggatt tggcagaaga
     5461 taagacaaat ccatcgatct ctagtaaaat aggcaaggaa ctttttgtcg cttgataaga
     5521 aaagacaaaa aaaggaggaa acgaaccatg acattaacag ctgaagtcat tgtggacgta
     5581 cctactatgc agaccgatca gccattcact tatttggttc cctctgaaat ggaaacagct
     5641 ttgcaagtcg ggatgcgagt agaagtccct tttggtaatg gcaatcgtca tgtccaagga
     5701 tttgttatgg ctatccaaaa atcagaagaa tcagctaatc catctttgaa ggcagttatt
     5761 cgtttgctag accttgctcc ggtggtcaat gaagaattac tttcattagc tgattatatg
     5821 aaaaaaatta cttacgcatt caaaatcact tgtctacaaa cgatgcttcc gagtgtgatg
     5881 aaagcagaat atgataaatt gatctatcct ttggcagaca caccagaagt tcaggtattg
     5941 tttgatcagc gagaagtgat ttcctggaag gaagcagaag aagaaggcag cctttctcaa
     6001 ttgattcgct ggcgccaaga gcaattagta gatatcaaat atgaagtcca tacacgaaat
     6061 aaagtaaaaa cgattcgttt ggtccgttct ttgttaactg aaaagcaaat cgaagaagaa
     6121 tgggcaaaat tacggcaaaa tgccaagaaa caaaaagaac ttcttttgtg tttatctgaa
     6181 ctgagccaag aagaaccaat tgcttatttc aaaaataaag gaatcagtac tgctgtcttg
     6241 aatcaaggaa aagaaaaggg ctggctggag tttatagaat cagaacgtta tcgcgaccct
     6301 tataaagacc gagtatttga ccaaacgacg gcattggaac tgaatgcaga acaaaaaaat
     6361 gctgtcgaac aaatcatcac agcaggtcag cagcaaaaag atcaggtctt tttgctggag
     6421 ggaatcactg gcagcgggaa aacagaagta tatcttcagg caatcgctga tgttttgtca
     6481 gaaaataaaa cggctattat gttagtgcct gaaatcgcat tgactccgca aatggtcgag
     6541 cgattcaaag gacgcttcgg agaatcagtt gcggtcttac atagcggact ttcccaaggt
     6601 gaaaaatatg atgaatggcg caagattgag cgggaagaag cgcaagtagt agtaggcgca
     6661 cgatctgcta tttttgcccc gctgaaaaat atcgggctga tcattatcga tgaagaacat
     6721 gaatcaagtt acaaacaaga tgagacaccg cgttatcatg cgagagatct agcaatttgg
     6781 cgcagtaaat atcatcattg cccaatagtt ctaggaagcg cgacgccctc tttagaatca
     6841 agagcacgag ctcaaaaagg cgtttatcag ttattgcagt taaaccaccg agccaaagca
     6901 gctgcacagc ttccagctat tgagatcgta gatatgcgtg aagagtttca gaatcatcgg
     6961 acctctactt tttctgctaa tttgcaggaa aaaatacaaa atcgattaga caaaaaagaa
     7021 caaactgtcc tactgctcaa tcgtcgagga tattcgtctt ttgtgatgtg tagggactgt
     7081 ggatttgttc ttccatgtcc taattgcgat atctctctta cactgcatat ggatacacgt
     7141 tcaatgcgct gccattattg cggacatgaa gaacctattc ccaatcgctg tcctaattgt
     7201 ggcggcaata aaatccgcta ctatggaaca ggtactcaaa aagtagaaga ggaactccga
     7261 gaactttatc cgcaagctag gattttgcgt atggatgtag ataccactcg aagaaaaggg
     7321 gcacacgaac agattttaca aaaattcggt gcaaaagaag cagatatatt attagggaca
     7381 caaatgattg ctaaaggttt ggactttcca gaagtgacat tagttggggt gttgaatgct
     7441 gatacctccc tgaatttacc ggattttcgt tctagtgagc atacattcca attgcttacc
     7501 caagtttcag gacgagcagg gcgagcggaa aaagcaggcg aagtcgtgat ccaaacattc
     7561 aatcctcaac attacgccat agaattggct aaaaaacaaa attatgagca gttttatcag
     7621 caggaaatgc atgtacggca tcgtggaggc tatcctcctt attactttac tgtgaaaata
     7681 accgccagcc atcctgaaga acaagtagcg gcaaagaaaa tctttcagat tgccaatcag
     7741 ctaaaagaag tgttaagccc tcaaagcttg ctattagggc caacgcccag catgattttg
     7801 agagtgaaaa accgatatta ttatcaacta atcatcaaat ataaacatga accgaatctt
     7861 cctcatgttt tggatgaaat tttgaatgga agccagaaag aacagcgaca aggtcttttt
     7921 gtggcaattg acaacgaacc attaaacttt atttaagaaa gggccaaatt atgcgctatc
     7981 ctatattgat ccaccctaat gataaattaa aaagaactgc tcagccaatc gatgtaatca
     8041 cggacgagac aatcgcttta ttagacaatt tatatgagac aatgattgcg aatgacggaa
     8101 tagggatcgc tgctcctcaa gtaggacaaa acaagagaat tgctgtgatc gaagtagatg
     8161 aaggcgagaa atttgaactg attaacccag aaatcatcga agcaaaaggt gaaagccttg
     8221 acgtagaagg ttgtctcagt attccgcatg tctatggaac ggtcaagcga gcagatgaag
     8281 tgaccgtgcg ttattatgat cgagacggag aagagataga agtgacggct tttggctatt
     8341 tagcccgtgc cttccagcat gaaatcgatc acttagacgg tatcttgttc attgagaaaa
     8401 tgatacagca gatcccggaa gaagaacttg aagaatatat ggaggaacat cttgatgact
     8461 aagctagtat ttatgggcac acctgctttt tctgtgccga ttcttgaagg gctgatagaa
     8521 tcaggttacg agatccaggc agtggtaaca cagcccgacc gtccagttgg gcggaaacga
     8581 gtaattacgc cgacaccggt aaaaaaagcg gctctgaagc acggtatccg tgtgctgcag
     8641 ccagagaaaa tctccggttc gccagaaatg gaagaaatca ttgaattggt gcctgatgtg
     8701 atcgtcactg cggcatttgg ccaattcttg cctgaaaagt tattgcaagt accaaaatta
     8761 ggggcaatca atgtccatgc ttctttactt cctaaatatc ggggaggtgc gcctgttcat
     8821 tatgccatca tgaatgggga gaaagaaact ggtgtaacga tcatggaaat gatcaaaaaa
     8881 atggatgcag gcggaatcta tgcgcaagaa agcataccaa tcactaaaca agatgatgta
     8941 gggacgatgt ttgaaaagct tagcctttta ggaagaaagc tattgctaga aacattgccc
     9001 aatattttgg atggacaaaa accagtacct caagacgagt cagaagcaac tttttcgcca
     9061 aacatcacac gggaacaaga agcaatcgat tggcataaaa ctgcagaaga aattgacaac
     9121 caagtacgcg gaatgcgtcc ttggccaatt gcattcacga cctatgaggg catacgctgg
     9181 aaattattat cggttgaagt attggcagaa actacagaca aagcacctgg tacgatcatc
     9241 cggaaagaca aaaaaaatct atggattgct tgcggtaaac agaccgtctt agccatcaat
     9301 gaattacagc cagctggtaa aggaaaacag gccgtacatg atttccttaa cggaagcggt
     9361 cagcaagttc agattggtca gcaggtgaaa taatgaaaaa aatccctaaa aaaatgaaaa
     9421 aatcagtacg atatatggca ttactgacat tagagcgagt gaacaagggt ggcgcctact
     9481 cgaatctttt gttgaatgag atgatcaaac aaagcgagct aaatcaaaaa gatgtgggtt
     9541 tatttacaga attgatctat ggtacgatta gccatcagcg actattggaa ttttacgttg
     9601 caccattgat cgctaaagca aaaaaagtgg atgactgggt caagatgctt ctttatcttt
     9661 ctgtatacca attagaattt ttagataaag tccctgcaca tgctatttta aacgaggcgg
     9721 tcgaaatcgc aaaagtaaaa ggaaatcctg ggacagggaa atttgtaaat ggtgtcttga
     9781 ggaattatca gcgtcaagga gcaccagatc tgcaaggcat tgctgatcct atcgaaagac
     9841 tttctgtcaa aatcagcctg cctctatgga tgacgaaacg actggtggat caaatcggat
     9901 acgaagaaac tgaaaagctc ggtttatcgc tatatcagcc tagccatgct agtgcccgag
     9961 tagatacacg ccgactttcc agagatcagg ccatcactgt tttgcaagaa gaagagatca
    10021 cagctgaaaa aagccaagtc agtccatacg gtatagtagc gaaaaaaggg catttagctt
    10081 ctagctcatt gttccacgat ggcgtcatga cgatccaaga tgaaagttcg atgcttgtag
    10141 cgccgtctat gcaaatcgag cctgaacatc atgtcttaga tgcctgtgca gcacccggag
    10201 gtaagacgac gcatattgct tcttttttag atccgtcatg cggtggagaa gtggtcgctt
    10261 tagatgtaca cgaacacaaa gtcaaactca ttgaagataa tgcagcacgt ctaggtgtct
    10321 cttctgctgt ctacgctcaa aagatggatg ctagagaagt cagtaaacag ttcgaagcag
    10381 agcagtttga tcggatttta gtagacgcgc cttgttcagg tcttgggctg cttcggcgaa
    10441 aaccagatat tcgttataaa aaacagccgg atgaattagc gaatttacca aaaatccaat
    10501 tggaaatttt agaaagctgc gcaccaactt taaagtcttc cggtattctg acttatagca
    10561 cttgtacgat cttaaaagaa gaaaaccaag acgttgtttc tgctttcttg gaaagacacc
    10621 ctgattttga gaggattgat gttttgacca atcctatgct acaatcatca atcgaagaga
    10681 aaatgttgac tttgtatccg catcagtttt acacagatgg atttttcatt tgttgtttgc
    10741 gcaaaaagta atgaggtgaa aaaatgcaga ttgaatatca atcagatgta ggaagacgac
    10801 gtaacacaaa tcaagactat gccagtgtat ttaccagccg ttcaggtctc aagcttgctg
    10861 tattagcaga cgggatgggt ggtcatcgtg ccggagacat tgccagtcag atggcggtaa
    10921 cgaatctagg cgaagcttgg gaaaaacaag aagctgttga cgatgaaaaa atcgcccaat
    10981 ggttcattca atcgattcaa gaagaaaata tgctgattta tcagcgtgga caagaacagc
    11041 cggaatataa cggtatggga acaacaatcg tagcggccgc tctgtcagat gactggttca
    11101 cgattgccaa tgttggggat agcagagctt atttgattcg tgaagataaa atcatgcaat
    11161 tgacggagga tcattcttta gtcaatgaac tagtcaaatc aggagaaatc agcgaagaaa
    11221 tggcagtgaa ccatccaagg aaaaatattt taacacgttc tgtcggcatg cctggaacag
    11281 tcgaagtaga tgtttctact catctttggc aactaaaaga tcgactgctg ttatgttcag
    11341 atggcttgac gaacatgctg tctgaagaag aaataggaac gatcatcaat caagaaggcc
    11401 cattgtctca taaagtaact gagctgattg atcgtgccaa tgaagcaggc ggagcggata
    11461 atatcaccgt tcttcttatc gaatataagg aggacagtgt atgatcgagt tgggcaaaaa
    11521 attaaatggc cgttatcaaa tcactggaaa tatcggtagc ggtggtatgg caaatgtatt
    11581 tttggcgcac gatttaatct tggatcgtga tgtagcagtt aaagtcttac gatttgactt
    11641 tcaaaatgac cagactgcga ttcgacgatt tcagagagaa gctttagcag caacagaact
    11701 cgttcatcca aacattgtta gtgtttacga tgttggtgaa gaagacaaca tgcagtatct
    11761 ggtgatggaa tacgtcaaag gaatggactt gaaacgatat atccaaacac attatcctgt
    11821 tccttatgaa acagcagtaa atattatgca gcagatcctt tctgctattt ctttagctca
    11881 taaccatcaa atcatccacc gagatttgaa accccaaaac gtactgatcg ataatgaagg
    11941 tgttgtaaaa atcacggatt tcgggattgc tatcgcttta tctgaaacat cgatcacgca
    12001 gacgaatacg atgctaggat ctgttcatta tctgtcccct gagcaggcca gaggaagtat
    12061 ggcaacgaag cagtcagata tttatgcttt agggatcatc ctttatgaga tgttgacggg
    12121 tagcgtaccg tttgatggtg aatctgctgt aacaattgct ttaaaacatt ttcaagatga
    12181 tcttccatct ataaaagcac ttgatccaaa tgtgccacaa gcactggaaa acgtgatttt
    12241 acgggcaacg gctaaagaac ctgcagaccg ttataagtca gccgaggaaa tgtctgatga
    12301 tctttctaca gtattatcac cagctcgtgc aaacgaagaa aaatggcagc ctcatgtgat
    12361 ggataatgaa acaaaagtta tcacacccct tacggaagat gcaccaatgc ctgattcatt
    12421 caaatctatg cctctgccaa aagacaagcc agaaaacgaa accttaccag aggaggaaga
    12481 agttctgcct gatcctaaaa agaaaggaca gaaaaaatgg tggctgatcc tattagctat
    12541 tcttgctgtt ttaggaatcg gaacagctgt attctttgct tctggcgggc gaggagaagt
    12601 tgttgttcct gatgtttctg atctttctga agcttctgct agagaaacac tcgcaaaagc
    12661 gaaattagaa gtagcaacaa aaacagaaga aattgcagat gacaaaatcg aagaaggtaa
    12721 tgtagtgaag acagacccgg cagcgggaac gacagtcaag caaaaacgcg aagtcactct
    12781 gtatattagt agcggcacga aaaaaatcaa attagatgat tacagtggta aaagttatga
    12841 agatgccgaa gcaagtttga aagaattagg gttttctgct gacttgattc aaaaagaaga
    12901 ggaattcagt tcagaggtag aaagcggaat gatcatttcg caatctccat cagaagatac
    12961 agaagtcgat cctaaaacag acacgattac tttcacagtc agcaaaggac cagaaacagt
    13021 gactttgagt agtcatgtag gagaaaacta cgaaactgtt cgtaattggc ttgttacaca
    13081 aggattcaat atcaataata ttagtgctac ttatgactat agcgacactg taccatcagg
    13141 ttcgatcatc agtcaggatc cagcctcggg tgaagttgta gcagaagaaa cttacatcaa
    13201 ttttgtcgta agccaaggaa cagagcctaa attgaatgat atctccggct atacaaaaag
    13261 cgaagcacaa agttatttag ctagtgtcgg tgcagaatat atcgggcatg agacttccga
    13321 atattcaaac acggtggata aagataaagt gattcgtaca gtaccagggg caggaacgac
    13381 aattaccaaa ggaatggttg tgaacgtaat ttactctaaa ggaccagatc cagcgtcttc
    13441 tagttctgaa aaagactctt cagacagttc tagcagctca agtagctcca gtagcacaag
    13501 caactcttca aaagaagaat cttccagctc cacaagttca tcagctagca cctcagaaag
    13561 ttcaaaataa gtgctgaata aaatatatca gaagggaatg cgacattgat ttgttgcatt
    13621 ctcttttttg tgtttctatc tcttttgatc aattttcaag ccatatactt tgagaagttt
    13681 gcatggaata acacctactt tcctcttcgt atggtaaaat aaacaaaaac ggaggtggca
    13741 cttatcaaag gacaaatcag aaaagcaatc agcggttttt actatgtata cgcaaacgga
    13801 gagacctatc aaacaagagg aagaggaaat ttccggaatc ggaaaatcac gccgcttgtc
    13861 ggagatcagg tgatctttga gagtgaaaat caaacagacg gctatcttct ggaaatcttg
    13921 ccgagaaaaa atcaattagt tcgcccacca gtcacaaatg ttgatcttgg aatcattgtc
    13981 acaagtatcg tagagcctgc attttcttat aatctacttg accgctttct agttaccttg
    14041 gaatacgagt cgatcgaacc gattattttt ttgacaaaga ccgacttgtc agacgaccca
    14101 gaaaaaatcg aagagatcaa gcaaacctat actaaaatag gctatccggt gattgtaccc
    14161 aatcatacgg gtgatacgac agaactgatc cgttacttcc cagaacggct atcggtcttt
    14221 atgggtcagt ctggagcagg aaaatcgacg ctactgaatc aaatatcacc ggaactgaat
    14281 ttagcaacag gagagatttc tgatgcactt ggaagaggac ggcatacaac acgtcatgtg
    14341 gaactgattc ctatctatgg cgggttagta gcagatacac caggatttag ctcgattgac
    14401 tttttgacaa ttgaagcagg agaactgccg agacaatttc ctgaatttgt agaagcttct
    14461 gtccattgca aatttagaga gtgcatgcat gctaaagaac ctggctgcga agtaaaacat
    14521 agagtagaaa caggtggtat cgcacagaca cgttacgaca attatttgca attcttacaa
    14581 gaaatcgaaa aaagaaaacc aatgtatgca aaaaaagaag gtaaacgaaa ataggaggaa
    14641 acaagaatga agatagcacc atccattttg agtgcagatt ttgccaattt acaaagagac
    14701 attgagttag tggaaaaagg aggcgcagat tacatccacg tagatgtaat ggatggccga
    14761 tttgtcccaa atatcacatt agggccaaat attgttcagg caatccgtcc tgttactaaa
    14821 ttaccgttag acgtccatct gatgatcgtt gatccagaaa actacattcc agcatttgca
    14881 aaagctggag cagatatcat tactgtccac gtcgaagcaa ctcctcatat tcatcgggca
    14941 cttcaaatga caaaaagctt aggagttaaa gctggagtag ttatcaatcc aggaacacca
    15001 gtctccatga tcaaacatgt tttgcctttg gctgatcaag tactagtcat gactgtcaat
    15061 cctgggttcg gcggacaaag ctttattgaa gaaacagtag aaaaaattac ggaactcgct
    15121 gatctacgta gagagaacaa ttggacttat gcaatcgaag tagatggcgg aatcgttcca
    15181 gaaacagcaa aaatctgtaa agatgcaggg gcggatgtct tcgttgcagg atcatatatc
    15241 tatggagccg aaaatccagt cggacaaatc gaacgattaa aagaggcatt agactaaatg
    15301 aaaattttat tagctgctgg aggtaatccg caaaactggc cgcagtctct gctgtccaaa
    15361 tacgatgcct atgtcgggat agaccgtggt gcgctgtacc tgcttcaagc aggctaccca
    15421 gtagatctgg cagtgggtga ttttgattca ttgtcgaacg aagagcagaa ggaagtattt
    15481 gcaaaagcca aagagtatac ccgttcccct gcagaaaaag acgatacaga tacacagtta
    15541 ggtatcttaa aaacatttga acgttacccg caagcggaag tcactttgat tggagctacg
    15601 ggcggaagat tggatcatct tttagccaat ctctggttag gtatggaacc tcgcttcaaa
    15661 ccctttgccc atcagcttat tatcagagat tgtcaaaatt atttgtctta ttatttgcct
    15721 ggagagcata tagtaagaca gataccagat atgaagtatt tagcttattg ctgcttgact
    15781 cccgtctcga atcttacatt aaaaggcagc aagtatgtat tggaaaataa agaagtagaa
    15841 tatccgacat cttatgcaag taacgaattt ctttcagata ccgcagaatt ttctttttca
    15901 gatggtatga ttgcagttat tcaaagcaaa gattgatcca taaacaaata aaaaaatttg
    15961 tctttcaggc taaaaatgac agaatctatt atttgctaaa aaaaagctta agaggagcaa
    16021 cttacctttt gtttcacggc atttagtgat aaaattgaac acaagagtct aattaattcg
    16081 aggtgtcttt gtgaagaagt tcaatcctaa taaaaatatt attattacct tggttattgt
    16141 tatcatcgtg gtgacgatac taagtgtgac cgctgcgcag cgagcgaatg aaggaaagcc
    16201 caatttcttc caatccatcg ttaatgacag tgttgcaatg gttgataaaa caatctctgc
    16261 cccagtacgg tgggtacgaa atggagtaga ttctgtccat aatctttttt caacttatgc
    16321 cgaaaatgag cggctaaaag aaaaaatcga tagctatgat gacgtttcac agcaaaacaa
    16381 gaattacgag agagaaatcg aagcgttgaa aaaagaactt gagttgaatc agacgctgac
    16441 tagttacgaa aaagtaacgg caaatgtcat tactcgttcg cctgacagtt ggcaggacat
    16501 gctgatcgta gacaagggtt ctaaagacgg tatcgaagca aatatggcag tcatgtcgca
    16561 aaaaggattg attggccgag tgatcgaggt caatacggct tcgtctaaaa tcgaattact
    16621 gtcatcctct aatgaaagct ccaatcattt tccagtacgg gtatcttcgg ctaatggcga
    16681 agcgtttggt ttgcttaaaa actatgatga aaagctccat gccttagtgg tgacccaatt
    16741 aactggtgat acggatatca aagaagggga tgttgtccag acatccggtc ttggagggaa
    16801 ttctccagct aacttgccga tcggtacggt tattaaaacg aaaccagata gttatgggct
    16861 ggatcgggaa gtttatgtga aaccttatgc agaaatgtat gacgtgtcag ttgtgacgat
    16921 tgtccaacgg ctagcggagg attagtccat gttgaagaaa gaaacaatga agtactatct
    16981 gccaatcgtt ttgttctttt tgatgttgat agatggtcat ttaacaagaa tgctagggga
    17041 gtggtcgaaa ggcacctata tgtcaaatgc ccactttctg atattggcat tattatgttg
    17101 cagtatggcg tttgaaaaac gttatttact gattaccacg attgttctcg gggctatcta
    17161 tgacgcttac tatattggcg ttatcggtat ctatgcagta gctctccctt taattgtatg
    17221 gttgatgtat gtaatgaaag acgttatcca tgtcaacatc tttactgaat ttttcagtat
    17281 gatcatcttt gtcacgggtt atgaattgtt tacgatggtg gtccagttga tttttaaatt
    17341 agcagtagta aataacacgt attttattac aaggttttta ggacctacac tgctgttgaa
    17401 catgattata tttgtattat tcatttttcc ctttaagaaa ttattcagtg atgaataatt
    17461 tcttttttta tttttaggtc gaaagcgaaa aagaatcgtt gttatcttaa gaaatgctag
    17521 acttaacgcg gctttttcat gatcattttc tgaaacgaac agttttgtca tttttaaaag
    17581 ttgttttgaa acatttttgt aacataacta ttatatcgtt gacacactgc tatggtacaa
    17641 tttttatatc gttaagaaag acttaaatat atttagatat ttattaaatt aagccagtcg
    17701 gaggaatgaa gagtgaaaaa gagtttaata tcagcagtaa tggtatgttc aatgaccttg
    17761 actgccgtag catcaccgat cgctgccgcg gcggacgatt ttgattctca gatacaacag
    17821 caagatcaaa agattgcaga cttgaaaaat caacaagcag atgcacaatc acaaatcgat
    17881 gccttagaaa gccaagtttc tgaaatcaac acacaagcac aagacctatt agcaaaacaa
    17941 gatacattgc gtcaagaatc tgcacaatta gtaaaagaca ttgcagattt acaagaacgt
    18001 attgaaaaac gtgaagatac aatccaaaaa caagcacgcg aagcacaagt aagcaacaca
    18061 agctcaaact acatcgatgc tgtattgaat gctgattcat tagcagatgc tattggacgc
    18121 gttcaagcaa tgacaacgat ggtaaaagca aacaacgact tgatggaaca acaaaaacaa
    18181 gataaaaaag ctgtagaaga caaaaaagct gaaaacgatg ctaaactaaa agaattagct
    18241 gaaaaccaag ctgctttgga atctcaaaaa ggcgatcttt tatctaaaca agctgatttg
    18301 aacgtactga aaacttcatt agctgctgaa caagcaactg ctgaagacaa gaaagctgac
    18361 ttgaaccgtc aaaaagcaga agctgaagca gagcaagctc gtattcgtga acaacaacgt
    18421 ctagcagaac aagctcgtca acaagctgca caagaaaaag ctgaaaaaga agcacgcgaa
    18481 caagcagagg ctgaagcaca agctactcaa gcttcatcta ctgcacaaag ctcagcaaca
    18541 gaagaatcat cagctacaca atcttcaatg actgaagaat catcatcagc tacacaatct
    18601 tcagcaacag aagaatcaac aacacctgaa tcttcaacag aagaatcaac agcgcctgaa
    18661 tcttcagcaa cagaagaatc aacaacagcg cctgaatctt cagcaacaga agaatcaaca
    18721 acagtgcctg aatcttcagc aacagaagaa tcaacaacag tgcctgaatc ttcaacaaca
    18781 gaagaatcaa caacacctgc gcctacaaca ccatcaacag atcaaagtgt tgatacagga
    18841 aacggcacag gaagtagtac tccggctcca acgccaacac caacacctga acaaccaaaa
    18901 cctgtaacac ctgctccagc tccttctgga agtgtgaatg gtgcagcaat cgtagcagaa
    18961 gcttacaaat atattggtac tccatatgtt tggggcggta aagatccaag tggctttgac
    19021 tgctcagggt tcacacgcta tgtttacatg caagtaactg gtcgtgacat cggtggttgg
    19081 acagttcctc aagaatcagc tggaacaaaa atttcagttt ctcaagcaaa agctggagac
    19141 ttgttattct gggggtcaca aggcggaact taccacgtag cgattgcttt aggcggagga
    19201 caatatatcc atgctcctca accaggcgaa agcgtaaaag ttggttcagt tcaatggttt
    19261 gcccctgact ttgctgtcag catgtaagaa tgat
//
LOCUS       DAZBSA010000052        18675 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000052 DAZBSA010000000
VERSION     DAZBSA010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.052"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            86..1603
                     /locus_tag="ACHGMT_001672"
     CDS             86..1603
                     /locus_tag="ACHGMT_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFN6835414.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            1575..2864
                     /locus_tag="ACHGMT_001673"
     CDS             1575..2864
                     /locus_tag="ACHGMT_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN6835415.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            3039..3935
                     /locus_tag="ACHGMT_001674"
     CDS             3039..3935
                     /locus_tag="ACHGMT_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6835416.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            3949..4812
                     /locus_tag="ACHGMT_001675"
     CDS             3949..4812
                     /locus_tag="ACHGMT_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6835417.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            4818..5381
                     /locus_tag="ACHGMT_001676"
     CDS             4818..5381
                     /locus_tag="ACHGMT_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFN6835418.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            5403..6914
                     /locus_tag="ACHGMT_001677"
     CDS             5403..6914
                     /locus_tag="ACHGMT_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFN6835419.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            6926..7885
                     /locus_tag="ACHGMT_001678"
     CDS             6926..7885
                     /locus_tag="ACHGMT_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFN6835420.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSKKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            7971..9050
                     /locus_tag="ACHGMT_001679"
     CDS             7971..9050
                     /locus_tag="ACHGMT_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN6835421.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(9302..10702)
                     /locus_tag="ACHGMT_001680"
     CDS             complement(9302..10702)
                     /locus_tag="ACHGMT_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFN6835422.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            10920..12581
                     /locus_tag="ACHGMT_001681"
     CDS             10920..12581
                     /locus_tag="ACHGMT_001681"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFN6835423.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            12538..13236
                     /locus_tag="ACHGMT_001682"
     CDS             12538..13236
                     /locus_tag="ACHGMT_001682"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFN6835424.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            13252..14676
                     /gene="araA"
                     /locus_tag="ACHGMT_001683"
     CDS             13252..14676
                     /gene="araA"
                     /locus_tag="ACHGMT_001683"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFN6835425.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            14983..16143
                     /locus_tag="ACHGMT_001684"
     CDS             14983..16143
                     /locus_tag="ACHGMT_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFN6835426.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(16120..16260)
                     /locus_tag="ACHGMT_001685"
     CDS             complement(16120..16260)
                     /locus_tag="ACHGMT_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFN6835427.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            16261..18120
                     /locus_tag="ACHGMT_001686"
     CDS             16261..18120
                     /locus_tag="ACHGMT_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFN6835428.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            18230..18664
                     /locus_tag="ACHGMT_001687"
     CDS             18230..18664
                     /locus_tag="ACHGMT_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835429.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
BASE COUNT         6180 a         3145 c         4006 g         5344 t
ORIGIN      
        1 aaaaataagt aagtttaaca tttatattat tgacaacgct ttcaacgagg tgtattattc
       61 atgtataaat taaaggaggt cagttatgga agccacaatg ttgattagta gtgtcaagcg
      121 tgtaggcaaa gttgatccgc ggttgtacag ttcatttatt gaacatatgg gacgagcagt
      181 atatgaaggg atttatcagc cagatcatcc atcagctaat aaaagtggat ttcgccaaga
      241 cgtcattcac ttggtaaaag aattgaacat tcccatgatt agatatcctg gtggaaattt
      301 tgtgtcagga tttcgttggg aagacagtat tgggcccata gcaaatagac ctagaaggtt
      361 ggatttagct tggcggacaa ttgaaacgaa tgaagttggg attcatgaat tttataactg
      421 gtgtcagcat gtgggggccg aaatcaatat ggctgtcaat ctgggtactc gtggagtaga
      481 tgcagctcgt aatttagtcg aatattgtaa ttttcctaaa ggaacttatt ggagtgattt
      541 aaggcgttcg aatggtcaag agagcccttt tggaattaag acatggtgtt tagggaatga
      601 aatggacggt ccttggcaga ttggacataa aacagcggaa gaatatggac ggttagcaga
      661 ggaaacggct aaggcgatga aactcgtaga tgattcaatt gaggtggttt tatgcggaag
      721 ttcgaatcgg caaatgccta catttggtga ttgggagtta acagttcttg atcaagcgta
      781 cgatcaaata gattatttgt ctctgcatca atattatggc aaccaaaaaa atgatctttc
      841 tcattatctt gcacgttcct tagacatgga tcggttcatt tctgaagtga cagcgatgtg
      901 cgatacagtc aaggcgaaga aacatagtaa gaagcagatc aacctgtcct ttgatgaatg
      961 gaatatttgg tatcattcag cggaacaaga tgagcaagca gcgccttggc agattgctcc
     1021 tcctttacta gaagataact ataattttga agatgcatta ctattaggct gtctattaat
     1081 cactttattg aaacatgcag atagagtaaa gattgcttgt cttgctcaat tggtaaatgt
     1141 tattgcacca atcaggacag aaacaaacgg agaagcttgg agacagacga tctattatcc
     1201 atttatgcaa gctgctgtgt atggacaagg agaggtgtta agcccacaaa ttcgttcagc
     1261 aaactatgcg acggaagatt ttgaagaagt gccgtattta gaaagtatag ccgtaataaa
     1321 tgacaaagaa cttgtcatat ttgcagttaa ccgtggagaa gaagaaatga atctaactgt
     1381 tcacttgtct gaattatcat tagagggagt gattgatttt tctgaaatga gtggatttga
     1441 tacaaaagca actaatcttc ctaaaagtga acaagtaaaa ccaagtcatt caacaaatgt
     1501 aaaagctgaa gatgggaaaa tcaattgttt attgaaaccg ctatcttgga atgtggtcag
     1561 atgtcgttta tcagatgaag gaggaacaaa aaatgaaagg taaaaaatgg ttaattggct
     1621 ctttgttggt agctgcaaca ggtattttag gagcttgcgg tggcggaagt tcagcagaat
     1681 ctgattctga taaagaagtg actttgactt tttggaatgg gtttactgca tctgatggtg
     1741 aaatattgca agaaatcgtt aatgaattta ataaaacaaa tgataaaaac atcaaaattg
     1801 aaatggatat catgacttgg gccaatttga atgaaaaact tcctacagcg atttcttcta
     1861 aaaaagcacc tgactttatt gcattaaatt atccagattt tgcacagtat gtagaaaacg
     1921 gagcggtaca accattagac gatttctggg aatatgatgg agtagataaa tctgatttta
     1981 aagaaacggc tattgaatta ggtgaagtag aaggtaaaca gtattttgtc cccatgcagg
     2041 tacaaggcat gtatatgtat tggaataatg atttgtttaa acaagctgga ttagatactg
     2101 aacaaccacc tactacttgg gaagaattga cagaaatggc accaaaatta actgatagta
     2161 gtaaaaatgt atcaggtttt gtcttcaata aggatggaac tgcaccgtta tataactgga
     2221 tgttagcaaa tggcggtaaa ttagtgaacg atgattatac taagtctgaa tttgcgtctc
     2281 ctgagaacct taaaacgtta aaagcgatcc aaaaaatgat ccatgtagac aagactggtc
     2341 cagaatcgat ttctggcgcc gaaatggaca atttaatgaa tgctggtcaa ctagccattg
     2401 aaatcaatgg gccatggtta aataacggat tgaaagcaaa cgagattgat tataatgtga
     2461 ctaccttacc acaagggagt gattcagaca aaaaaacagc tattttagat ggcgtaggat
     2521 atgccattcc ggcaagtaca gataataata aaaaagaagc tgtctatgag tttttgaaat
     2581 attggaatac aactgaaatt ggtaaaaaat ggagtatcga aaatggtttt cctgcctact
     2641 tggattcagt agcaaaagat gaagaagtaa aaaatgatcc tattgtatct gaattatcga
     2701 aacaaatgga ctatgcagag ccatatttac caggttttac ccaaatctct gcgatcaata
     2761 acgatatcat caatccactg attgaaaaat tattagcagg agaagatcct gagacactca
     2821 tgaacgatgc agatgatgcc atcaatcaat tactgcaaaa atagtacaga actttataca
     2881 ggatagactg ttggcaagat acagatatct gaatgatcga cctagtattt gcagataggt
     2941 gttattgcaa aaatcacagc gattgttcgg atatcttatt cagattcgtt taaaccaaga
     3001 ttgtatattt tatcaaaatg aaagatgagg tggactatat gaacggattc accaaatatt
     3061 ggaatcgtcc tgatcgtgct gcgtatttgt ttttaactcc atctttttta gttttattat
     3121 tatttactgt cataccgctt ttaggaacat ttgctattag ttttacagat ttgaatattt
     3181 tctttacatc aagaaatttt gtaagttttg aaaatttcac acgtatattt agtgatgaac
     3241 gagcagttaa ttcgttgatt cattctttat atttcaccgt actcgaaacg ccaacacaaa
     3301 ttatcgttgg tttattagca gcagtagcat tatctaaaaa taccagattc aatcgttttt
     3361 gtcgttcgac attttacatt ccagttattt gttcattgac gtcaattgcg attatttttt
     3421 ctatgttgct tgatccgaat gtaggggcga ttccttactt gttttcacaa gtcggcttgc
     3481 cgatccctca attttttcgc gatccaaccc ttgctatgcc gacggtagcc atcatgactg
     3541 tttggaagaa ttttggtgtt actatgacga ttctcttgac tgctattcag ggaatctctc
     3601 cctctttgta tgaatctgct caaatggatg gtgcaacaaa tcgacagcag ttttttaaca
     3661 ttacgctacc tcagatcgtt ccctccttag gtttttgtat tttaacgaat ctgattggtt
     3721 cgatgcaagt gtttgaccaa gtatatgttg caaccgctgg tggcccacaa tttaagacag
     3781 aaacagctgt tcaatatatt tacagtcgag gatttacagc accttatgaa ttgggctatg
     3841 catcagcact gtcttcggtt ttgtttatca ttgttgcaac tatagctatc tccactaatg
     3901 tgtacatgtc acggaaagaa aaacaaatga aataggaggg aaacctgtat ggcaccagaa
     3961 agctcgatta cagttactgt gaatagtcga aaagcagtta gtggtaaaaa aatcattcga
     4021 acgattagta tgctagttat attgttggtt attgcgtttc cttttttgtg gctgattatt
     4081 tcttctttta aacacgaaaa agacattatc tcatttccgc ctagaatatt tgcagatagc
     4141 tataccctag ataactatat taaggtttgg acaacgatcc ctttattaga ttacatcaaa
     4201 aatacagtga tttttgctgg agggacagtg atcacttctg tattctttga ttctcttgcg
     4261 gggtatgcat ttgctcgtat gcgtttcaaa ggaaaatcag ttttgttcta tttcgttttg
     4321 ctgacaatga tgatcccatt ccaagtcttc atgattcctc tatttattga agtcaatttg
     4381 cttggcatgc ttgatactta tgcaggtttg atcattcctc gtatgacaac tgcatttggt
     4441 atcttcatga tgcgttcctt ttttattact ttaccagatt ctttggaaga ggcagcacgc
     4501 attgatggat taagtgagtt taatattttt ttgaaaatca tgttgccatt gagcaaacct
     4561 actcttttat cattagggat tttcacgctg atgaatagtt ggaatgactt gctttatcca
     4621 ttgatattga caagtagttc aaaaatgaga actttaccag cagggttagc tttatttaca
     4681 gggcagaaca tttcttttta tggacctgta atggctggaa cggttatttc aatgctgcca
     4741 ttactagtgg tttatatttt tgcacagaaa tattttgttc aaggaacagc tatgtctgga
     4801 atgaaagagt gaggaagatg aaaatagttg agactaaact ttatggacag ttgattgtgg
     4861 tttctgatta catcttatta ggtattctct gggtaatcgt gtgtttgcct gttattacta
     4921 tcgtgcctgc tacatgtgct gttttttatg taatggcgca atggaaaggt gagggatcag
     4981 gaaatatcat ttattttttc tttcaaggaa taaagcaaca ttttggtaag aacttgttcc
     5041 ttagtctgtt aacgatcatt atttattttc ttgttaatca attattagaa gaacagacaa
     5101 tcgtactttc aattagtgga tatgtagttt tacttattta ttccatgttt cttttatcct
     5161 ggatatatgt gatttctgaa aacaaagata tcacattttt acaaattttt gaacaaagtg
     5221 catggaaagt attttttgat tttttgagaa atattctttg ttgcgtattg ttattaggat
     5281 ttgtactgct catatttctt ttccctcctt ttatttttat ctttgctggt ggcgtatgga
     5341 aacttgtatc tattatcttg actataggaa atgaaaggta gggaaagctg ttggacgaag
     5401 aagtgaaagt aaaaatcaga gaggatttta cggtaggaga gatcagttct cggctgtttg
     5461 gctcatttgt tgagcatatg ggaagtgtcg tctatacagg gatttatgaa ccgggccatg
     5521 aaacagctga tggtgacggg tttagacaag atgtcctctc ttatgtaaag gaattagaat
     5581 taggtgtcat tcgctatcct ggaggaaact ttacatctgg ttatgattgg gtggatactg
     5641 taggtaggaa agaacaacga ccaaaaaaaa ttgatttagc atggcgaggt atagaaccaa
     5701 attcttttgg actccacgaa ttttttaaat ggatcagtca tgtacaagcg aaaccgatat
     5761 tcacggtgaa tttaggaaca aaaggaattg atgaagcaaa aaatttagta gaatattgta
     5821 attttccaaa aggaaccttc tggagtgatt taagaataaa aaatgggcaa aaaaagcctt
     5881 ttggcgttaa attatggtgt ttagggaatg agctagacgg tccatggcaa gtgggggcta
     5941 agagtgctta tgaatatggt aggttagcaa atgaagcctc aaaagtcatg aagttagtgg
     6001 atgattcaat tgaaacaatc ttagttggaa gctctacgcc acgtttacca tcgtaccctg
     6061 aatgggatcg tatcgcatta gaagaagctt atgagaatgt agattattta gctttgcata
     6121 actatattga taagtatgat gaagaagact tgactaaacc tcctaaaaat gagcgtgatg
     6181 atacagcgac ataccttgca aaagcttatc gattcgatcg tcaaattgaa gaaatcgttg
     6241 ctacttgtga ttatgtaaaa ggatatttga gaagtgagaa aacaatgtat ctagcgtttg
     6301 atgaatggaa tgtacatgcc caaccggaaa aaagacatag ggattttgaa gtcggtagtc
     6361 ccatcgattg gtgttacttt actatggaag atacattatt atttggttca ttagggctgg
     6421 ctattttacg acacgcagat cgcgtaaaga ttagttgtca atcattgtta gtaaacacaa
     6481 tacctttaat actgacagat gctaaaggac aagcatggag gaatccaact tattatgtga
     6541 tgcaacactt atcgaaatat gctaaaggtc aagtattgaa acaacaaatc atttgccctg
     6601 tttatgattg tgaaaagata aaaaatgttc cttatgtgga cagtgtgatc gtagataacg
     6661 gcaatcagct gactattttc ttgatcaatc gcacaaatga acaacaaaag ataacgattg
     6721 aacatgattt caatttgcta gatcaaggag agcactacac attaaccagt agcgatctgt
     6781 tggacaaaaa tacacgtgaa gaaccagaaa aaataaaacc tgaaataaac tatttttcaa
     6841 tagaaaaaca tgacgaatta aatgtaggga tagaaaaata ctcttggaat gtaattagaa
     6901 taaagaaaat gtgaggaaat ttgttatgat gaagacactt tataataatc ctatcgttcg
     6961 gcaacgggca gatccttggg tatataaaca tacagatggt tattattatt ttacaggatc
     7021 ggttccaggc taccaagtga ttgaattaag aagggcaaag tcactgaatg aattagaaca
     7081 tgctgcaagc ctgattgtat ggcaggctca tgaagaagga ccgatgagtg aattgatctg
     7141 ggcacctgaa gtccattata tcaatggaaa atggtatatt tactttgctg caagcaatca
     7201 taaaactata cgtgatgaat ctcatcatca ccgcatgttc gtacttgaaa atcagcatat
     7261 agatccaatg aatacagatt ggatagaaaa gggacaaata aaaacgaaaa gggaaacttt
     7321 cagtttagac ggaacaactt tctcttctca aggaaagcta tattatgtgt gggcccaaca
     7381 agatcctgaa attactggga attctaatct ttatctatcc gaaatggata atccttggac
     7441 gttaaagggg aagcagatcc tcctttctat acctgagtat gaatgggaaa aacggggttt
     7501 ttcagtaaat gaaggtccag cagtccttac tcgcaatggg aaaatattca ttacgtattc
     7561 tggtagtgca acagatgaaa actatgccat gggcctgctg tgggctgatg agcacaagaa
     7621 tcttttatta aaagaatcat ggcataaatt atcggagcca gtattcgttt cttctaaaaa
     7681 aaataagcaa tttggtcctg gtcacaactc gtttacggtt tcagaagacg ggtgctttga
     7741 tattttgatc tatcatgctc gaccagagaa gaaccaaaaa ggggatccgt tagacaatcc
     7801 taatcggcac gcgaacgcgc aatgctttac gtggaatgag caaggatttc cagaatttgg
     7861 tgaacctgtt ccgtatgcct tatgagcaca agaaaccttg atttttgctt gaataaacct
     7921 tgcagactcg ttatgatata tgtacgtaca aataaaaagg ggcaaggcaa atggcaaaat
     7981 atcaagatat tgcagaagag ataaaacaaa agatccttcg tgaggaatac gaagtaaata
     8041 caaccattcc ctcagaattc aagctgcaag aaatatatga cgtcagcaga catacgattc
     8101 ggcaagcaat cgctgtgttg gtaaatgagg gctatctgcg taaagaaaaa gggtcgggca
     8161 catatgtaga tgacagctat aagaaagaaa aaacaccatt gaacagaaca aaaacgatcg
     8221 gtgtcatcac cacatatctc tctgattata tttttccgtc gattatccga ggaatcgaaa
     8281 atgaattacg agaatctggg tattcattgc ttttagccag tacgaataac gatcacgaat
     8341 tggagaaaga gtgtttgaag aaaatgattg cttatggggt agatggtttg atcgttgagc
     8401 ctacgaaaag taatcagtat aacccaaact tagcacttta tgttgctttg agagaacaag
     8461 gcattccgat ggtgatgatc aatgctgctt atgaagagtt aaatgcctct tatatttgtt
     8521 tagatgatgt gaaagtcggt tttatggcag cagaatattt gataaaaaaa ggacatcagc
     8581 gtctgttgtt catcacaaaa atcgacgatc ttcaaggaaa atacaggatg aaaggtttta
     8641 tcagcgcgtg tgaacagtat aatgtgcgtt ttttgacgga agatatcatt actttcacca
     8701 cggaaacacg agaaacaatc gctgaaaaaa cagtacaacg gttgaaagaa gaacaagctt
     8761 tagatggcat catttgctat aacgatcaaa tagctagttt aatgctagat aagttgacag
     8821 aaaatggcta tcatattcca gatgacctat cgatcgtagg aacagacgat tcttctttaa
     8881 gccatttagg gaataaaaaa ttgacaacat tgacgcatcc aaaagaaaag ttgggacaag
     8941 atgccgcacg gtggatcgtc acaacgattg aaaaaggaca ggcacagcca aatattctct
     9001 atgaacctgt actagttgaa cgggaaacag tgaaagaaaa cgaaaaataa gagacgggtt
     9061 tttaccgcct cttattgctc ctacggttac tcgtcgcaat gtttgtgaga gcttttgtca
     9121 tcgctcaagg ctgaacgttt ctttcaccac ctctccacta aacggtgttc tcctaccaac
     9181 tggtcacaag tcacaatatt catcaaacat gagaagctgt tttttgaaat tgttccttgt
     9241 gactggaaac taagcggttc cttcttattt cttgaagctg accacttctg tcacaacctc
     9301 tttaagaatg atggatagaa atacgtttag gataagtttt tttagatgca ctttgttcga
     9361 tttcctctaa agatttccca cgagtttctg gtacgatctc ttgaataaat aaaaccccta
     9421 aaaagcagat gatcccaaaa atgccgaaaa cgatttcttg agacatagaa gcagtcatca
     9481 caggaaaaag caagccaact aggaaagaac caatccaatt aaaagaagag gctaggccag
     9541 atgctcgccc gcggacagct agtgggaata tttcgcctac gattacccat gtcagtggag
     9601 cccatgtgca agaataaaat gcgacataaa tacataaaaa caagagaatc aacaacgaat
     9661 ttgtatgagc atccagtaca gttcctaaaa cagctggtag gatgaaagaa agtcccatca
     9721 cgctaccgcc tattttcaat aaagttcgcc gattgaactt gtctgcaata accaaaaaga
     9781 gcaaagaacc agcaactaaa atgattcctt ggatgatcgg ccacatcaat gcatcgctcg
     9841 ctgcatgccc agtcgctttt tctacgatca gcggaatata gtaaaaaata gcattagctc
     9901 cttgaaattg ctggaatact gctacaccaa taccggcaat gactaaggaa cgatattttt
     9961 ctaaaaacaa agtatgccaa gaagttccgg ttgtcttttc ttgtttgaca ttcagctgga
    10021 tttgttcgaa ttcctgttca gcttcttttg gtgtccgaat atagttcaat actctttttg
    10081 cctcttccaa gcggccagat tgcataagaa aacgcggtga ctctggtaga gctaaaacac
    10141 cgaaaaataa aatcagcgcg gggacagcag cgagccctaa catcacgcgc caagccatcg
    10201 tttcaggcaa cccttttagc aaataatctg caacatagga aaggagcatg ccagaagcaa
    10261 tcatcacttg attgatccct gacaatcttc cccttagtct agcaggagcc atttcagaca
    10321 tataagcagg aactagggca gatgcagcgc caacagctaa acctagaaga atccttgctg
    10381 caatcagaaa ataagaacca ttgtgagggg cagatgcaga aaggacagaa ccagccataa
    10441 aaataagtgc agaaagaaga atcatttttc gtcgtccaag cttatcagaa agaagaccag
    10501 ataaagagcc gccaaggatc gcacctaaca tgaccgaaga cgtgatccat cctatcaaag
    10561 aagctttacc tgccaaaccc caatcatgct gtaaaaaagg taaggcgccg gtcataacac
    10621 caatatcata tccaaataga atgccgccaa atgcgccaaa aaaataaata aacgatgagg
    10681 aaactttttt ttcttttttc ataagtattc tctccttcta gaatttcgag aaggcggttg
    10741 atcagatttt gtaagcaatt tcattttata acttatgtac gtacaaaaca agtgtttttt
    10801 attgtattct tgtaaataac tgcttgacat gtacgtacaa aagtatacaa taatcattgt
    10861 aagcgctact aaaaagacgg tttgatcagg tttgacgaga aggagggagt taaatcatta
    10921 tgaaggaagt aaatgcgcaa agtatgttag atgtcataga tggtcgtaca tctttaggga
    10981 ttgagtttgg atcaactagg atcaaagctg tattgattga ttcatctttt actcctattg
    11041 ccgctggtag ttatgagtgg gaaaacaaac tagaaaatgg tatctggacc tattcattag
    11101 aagatatttg gaaaggactg cgcgtcagtt accgaaaaat ggcagcagaa gtattcgaaa
    11161 tctatggcga gaatcttacg acaatcgggt cgattgggtt cagtgcaatg atgcacggtt
    11221 atctagcatt tgatagcaag gaacagttac tagtaccatt tcgtacttgg cgaaatacaa
    11281 tcactgaaga agcagaacga atattaacgg aaaaatttca atacaatatt ccgcagagat
    11341 ggagtatcgc tcatttgtat caatctattt tgaataaaga agaacatgtg aaagaaatta
    11401 attttttgac aacgcttgca ggttatgtcc attggcagct tactggtgag aaagtactag
    11461 gcgtaggaga tgcatcaggc atgtttccta tcgatccttc aacgaaaacc taccatcaag
    11521 aaatgaaaaa tatttttggt cagttaatcg aaaaagcgca cgtagcgatt gacttggatc
    11581 acattttgcc taaagttcta gtagcaggag aagaagctgg caagttaagc gaatcagggg
    11641 cttatctgat cgaccctacg ggaaatttga gaccaggaat cccggtttgt ccgccagaag
    11701 gggatgcagg aaccggtatg gtcgctacga acagtgtagc caaagggaca gggaacattt
    11761 cggctggtac ttccgccttc gctatgatcg tattagaaaa agaactagaa aatgtccatc
    11821 cggaaatcga catggtcact acaccttcag gagaactagt aggcatggtt catacgaata
    11881 actgctcttc ggatatcaat gcttgggtca gtatttttga agaatttacc aattctttgg
    11941 gtatggagat aagcagagat aaattattca cggtattatt caacaaagcg ctagaaggcg
    12001 atacggattg cgggggatta ctttcttatg gttatttttc cggtgaaaac attacaggag
    12061 taaatgaagg aaggccatta tttgtacgta caccaggtag ccgttttgat ttagcgaatt
    12121 tcatgcggat ccatttagcc agtgcttttg gtgcaatgag aatcggaatg gatatattga
    12181 aatctgagaa tgtccaaatt gatcgtttag tggggcatgg ggggattttt aaaacaccag
    12241 aagttggaca gcggattcta gcatctgcta tggaggcacc agttacagtg atggacacag
    12301 ccggagaagg tggagcgtgg ggaatcgcgt tattagcagc ctatatgatg gatcgacatg
    12361 ggaagtcttt agaaaacttc ttggcagaag acgtgtttgg acaagaccaa ggaatcacca
    12421 tcacaccttc agcagaagaa atagcaggct atcagatatt tatgagacgt taccgtgagg
    12481 ggatcgacat tgaaaaaaca gcgattcatc aattgcaaga aaaaggagtg atagaaaatg
    12541 ctggaacaac tgaaagaaga agtataccaa gcaaatcttg acttgcctaa acacggactc
    12601 gtcaaatata catggggaaa tgtgagcgct tttgatcctg aaacacgata tttcgttatc
    12661 aaacctagcg gagtcagtta tgaagagatg acagcagacg acatggtcgt tgtggacttg
    12721 gacaatcata tcattgaagg taaactcaat ccctcttcgg atacgcctac tcatgctgtt
    12781 ttatatcgtt catttcctca aatcggagga atcgtccata ctcactcgac atgggcgacg
    12841 atctgggcac aagcaggatt ggatgttcct gcgatgggga cgacgcatgc agatactttt
    12901 tatggatcgg taccttgtgc tcgatttttg acacaacagg aaatcgacag cgggtacgaa
    12961 tatgaaacag gaaaagttat catagaaaca ttcaaagaac gcaaaattga tcccttagca
    13021 attccagggg tacttttaca cggacacggt ccatttactt gggggaaaga tgcgcaaagt
    13081 gcggtaatga acgcagtcgt actagatgaa gtatgcaaga tgaatttatt taccagacag
    13141 ctcaattcat tttcagaaga gctgcctcaa cggattttag ataaacatta tttacgaaaa
    13201 cacggaaaaa atgcgtatta cggacaaaac aaataaaagg agtggctaat tatgttgaat
    13261 attggagaaa aagaattttg gtttgttgta ggatcacagc atttatatgg ggaagaagca
    13321 ctgcgagaag taaagaaaca tgcacaagaa atggtcgatg aactgaatga aaatggccaa
    13381 ttgccttatc caatccgcct tcaagaatta gcagttactg ctgatacgat cacaaaaatc
    13441 atgaaagagg tcaattatcg agaagaagta gcaggtgtga tcacttggat gcatactttc
    13501 tcaccagcaa aaatgtggat ccgcgggaca aagcttctac aaaaaccatt gcttcattta
    13561 gctacgcaat ataacgaaag cattccttgg aaaacgatcg atatggattt catgaattta
    13621 aatcaatccg ctcatggtga ccgcgaatat gggttcatca atgctcgatt aaataaacaa
    13681 aataaaatcg tcgttggcta ctggaaacga cctgagatcc aaaaagagat tgctgattgg
    13741 atggatgttg cagtagccta taatgaaagt tttggaataa aagtggctcg ttttggtgac
    13801 aacatgagaa acgtgggtgt cacagaagga gacaaagtgg aagcacagat tcaatttggt
    13861 tggactgttg attattttgg tatcggagat cttgtacaag taatcgatcg cgtatcagac
    13921 gaagaagtcg atcaattatt tgaagagtac aaggaattgt ataccttcga ctatggtgat
    13981 tatgaagaaa agacatggga ggaacatgta aaagtacaag ctcagcagga aatcggtatt
    14041 cgccgtttct tagaagaagg aggctataac gcttttacga ctaattttga agacctgtat
    14101 ggaatgaaac aactacctgg actagctgta caacgtttga tggcagaagg atacggtttt
    14161 gctggggaag gcgactggaa aactgcagcg attgatcggt tgctgaaaat catggctcga
    14221 gggaaagata caggattcat ggaagattat acttacgagc tagctagtgg ccaagaagca
    14281 atcttggaat cccatatgat ggaagtagat ccgacgcttg ctgcgacgaa accaagaatc
    14341 gttgtttctc ctttatcaat gggagataga gaagatccgg cgcgtcttgt atttgatggg
    14401 aaagcaggag aaggggtagt cgtatcgatg gcagatttcg gtacgcatta taaactgctg
    14461 atcaatgaag tagaagcctt tgaaccaacg actgaagcac ctaatttacc tgttgcacgt
    14521 gtgttatgga aaactaaacc gaatttccat gaaggtgtcc attcatggat tcaggcaggt
    14581 ggcggtcatc ataccgttgt ctcgctaaat ctaacaacag atcaaattga aacgtgggca
    14641 aaattagttg agttggaaac agtggttatt agataaaaag aggttgtgaa aaagccactt
    14701 agttttaaga aataagaaga gattgacgaa atttgctttt caactttttt gaaatttagt
    14761 cttattatca atgtcagcta acggaacacc atttatcaag atatctcacc ctcaatattt
    14821 tgctctcgaa aataaggctt atggcagtaa cttgtcatag ccttattttt atcactcagc
    14881 ctactctgct ctaactgctc tttttgcttc taggaagtca aatggtaaac agttgtctaa
    14941 ttgcagaaat ttcgagatac ttgttagtaa ggagggggtt ttatgtatca cgttcatttg
    15001 attgatgcta ttccagaaca agagatagaa aagttacaag gggaatattt caaaataata
    15061 gatgatttgc cagccgaagg aatagttatt cgtagtacga aagtgaaaga tgaatggctt
    15121 acaccagatt tgctagcaat ttcaagagct ggtgttgggg tgaacacgat caacgttgaa
    15181 aaagcttctg aaaacgggac gatcgtcatg aatacaccag gtgtaaatgc taatgcagta
    15241 aaagagcttg ttttgtgctg tttgctctta tcttcccgtc cgataatcga agcaagtcgt
    15301 atggtacaaa cactaacagg tcctaatatt ttagaacagg cagaaaacaa aagaagtgct
    15361 tatgtgggac gagaattgca aggcaaaacc atcggtcttc ttggtcttgg agcgataggc
    15421 acaaaagtag cgctaagttg ctatagctta gggatggatg tcttgggtta ttctatccgc
    15481 gatgcccagt tggattatgt tcggcaagca gatttagaaa cagttctttc tacctcggat
    15541 tacatcgtag tcatgctgcc gttgacagaa gatacaaaag gattgatcga tcaggcgaat
    15601 atagaaaaaa tgaaaaaaga tgctgtactt atcaatgtag gaagaagtga aattgttgat
    15661 aaatatgctg ttatgcaagc attggaaaaa caacatctgg ctaaatactt gactgatttt
    15721 ccagaagaag agttcttaga aaatgaccgt atacttatgt tgcctcatct agggggatcg
    15781 actcaggaag ctttggcaga cagcggacgt ttggcagttg aagccttaaa agattatctt
    15841 ttgttcggta cggttagaga agcggtcaat tatccatccg cacgaatgtt tttccaagcg
    15901 ccgtttcgat tcactatatt ttatcaaaag aaaaccaata tattagcaga gatattttct
    15961 ctgctgaatg agaacgagct agcgatcgca gatatatcgc gtaaccataa gaatgagcat
    16021 gtctacatat tgattgacat agacagtact gattttgaac agctcaatca agtgaaacaa
    16081 cagctagaaa aaatttctgg agtacgaaag gtccgcttat taaagaaacc agaaaggcca
    16141 taaagaaaga gaatatttgg atttcattaa ttcttctttc cgttctttac tttcgaattt
    16201 cgtacaacta aaataagaga agcaacattc ttttattgat agaaatgaag gagcaagcat
    16261 ttgaaaagat atataagcag actttcgacc aaacagaaaa agattttaaa aggaacagca
    16321 gcaatcatca gtatcttttt tcttgttgtt tttagtaatt tatttttgca gtggtgtcaa
    16381 aatgatctat ccgtagacct tgcgttgaaa tttgcttttt cttggcatac ggaaaaattc
    16441 ttcttagcct gtctcgtctt gctgattatt cttatttttc tgattgcact agcaggatct
    16501 gtccctttag gttcccttac gtatgtcgta gccatcggtg tattaggttt tgcaaattat
    16561 atgaaaatga gttatcggca agaaccgatt tatccagacg atttgaagat gatcactgaa
    16621 atcggattgc tgaaagatat gaccggcact atgctgttta cagtgatctt agctgccgca
    16681 ggaaccgtga taggattgtt ttgctggtac atgtttcgaa gcctgaaaaa aggaaggcga
    16741 ttccaattga tccgattaac gacactgctt gtggcaattg gactgctagg gtatatcagt
    16801 aatttcaaca atcccgataa tttgttgcga aaagcatata ataaaacggc actatggata
    16861 ccttacagcc agaaaatgaa ctactataac actggtttta tcggtgggtt tttgtacaat
    16921 ctgaaggtag aaccaatgga tgaacctgaa ggatattcga aagaaaagat caaagaaatc
    16981 actgaaaaat accagaagct agccgatgaa aaaaataaaa ctgtagagga agagtcgccg
    17041 aacatcgtgt tcgtgatgag tgaaagtttc tcagatccta gtcgattaaa cggcgtagaa
    17101 gtatctggtg agccattggc agattattat gaagtagctg atcaaacata tagtggcaat
    17161 atgctttctc aaaactatgg cggtggaaca gcaaatatcg aatttgaagc attgactggt
    17221 ttttctatgg cgttatttaa tgcgcaactc accacccctt acacgatgct tgtaccaaaa
    17281 atggatcagc ttccttctat cgtttcagcg ttaaacgcac aatcatacca gacgacagcg
    17341 atccatccat acaatacttc gatgtataaa agagaggatg tttatcagac attaggattt
    17401 gaccagttca tcagcgagcg tacgatgacc tatacggata cgatagagaa taatccatat
    17461 atttctgatg agtcagctta caaagaagta atgacacttc tcaaggaaga aaaaacacct
    17521 cagttcattc atttagtcac gatgcagaca catatgccct acgatggaaa gtatgataat
    17581 ctagactatc ctgcaaaaac ttcagatgga agtgggacac ttgacttaga gaattatctg
    17641 caagatattt cctatagcag cacggcactg aagcagttca cggaagaatt gaagaatctt
    17701 tctagaagga ctttagttgt tttttggggg gatcatcttc cagggatcta ttcagatacg
    17761 atccaagcaa aaaatgacaa acaaacattg catgaaacac aatttcttat gttcgacagt
    17821 aaaggagaat tagaaaagca aacgactcac gatgcaatca ccagtccgtt ttattttgca
    17881 gcgaatctaa tggaacagac gaatcagaca acaaatggct tttatcagct gcttttatca
    17941 ttagaacagg aacttccggc gtttgaacga gaactgtact atcaaaacgg acagtggtat
    18001 aaagaagcgc aattcaatcg ctcgcaacaa gagatttacg acgaatatca attgatccaa
    18061 tatgacattg tcgcagggaa acaatatagt ttagcagatg gtttctttga gcacgaataa
    18121 gaatggcgag ctgtatagaa accattaaat tttttcaagt acttgtcatt tgaggataaa
    18181 aacaagtaga ataaataata acaacaatgg agaaagtagg aaaacgaaca tgttaccgaa
    18241 tattggctat tatgtgcagt ccatcgatca aatcgtcaag gaaacagaag caatcggaga
    18301 aggaatgcat ccgaaatatg aagtgatcag aaacgcaatt gatgaaggaa aaacaagtga
    18361 actggacaaa tcgactcttg aagagatcat cctgttattt gaagaaggaa caaaaaaata
    18421 tcgggcaatg cttatgaaag tcagtacttt gcgtccacca gcaaaagcaa tgggcattca
    18481 taaaaatttt gaaaaagctt atctttcata tgtagcaggc tgtgaagaaa tgatccaaag
    18541 tatcgatctt gaatcagggg tagatgaaga tctattcaat acatctgaag caaaacaaga
    18601 ccaagcgaca gaccaattga cagctgccat ccaaaaaatg actaatttgt tgttgaaaaa
    18661 ataaaagaaa tcttt
//
LOCUS       DAZBSA010000053        17778 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000053 DAZBSA010000000
VERSION     DAZBSA010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.053"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(57..1337)
                     /locus_tag="ACHGMT_001688"
     CDS             complement(57..1337)
                     /locus_tag="ACHGMT_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFN6835430.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            complement(1381..3414)
                     /locus_tag="ACHGMT_001689"
     CDS             complement(1381..3414)
                     /locus_tag="ACHGMT_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835431.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            complement(3448..4299)
                     /gene="rfbD"
                     /locus_tag="ACHGMT_001690"
     CDS             complement(3448..4299)
                     /gene="rfbD"
                     /locus_tag="ACHGMT_001690"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFN6835432.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            complement(4397..5425)
                     /gene="rfbB"
                     /locus_tag="ACHGMT_001691"
     CDS             complement(4397..5425)
                     /gene="rfbB"
                     /locus_tag="ACHGMT_001691"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFN6835433.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            complement(5447..6019)
                     /gene="rfbC"
                     /locus_tag="ACHGMT_001692"
     CDS             complement(5447..6019)
                     /gene="rfbC"
                     /locus_tag="ACHGMT_001692"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFN6835434.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            complement(6033..6899)
                     /gene="rfbA"
                     /locus_tag="ACHGMT_001693"
     CDS             complement(6033..6899)
                     /gene="rfbA"
                     /locus_tag="ACHGMT_001693"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFN6835435.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            complement(6999..7706)
                     /locus_tag="ACHGMT_001694"
     CDS             complement(6999..7706)
                     /locus_tag="ACHGMT_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN6835436.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            complement(7711..8538)
                     /locus_tag="ACHGMT_001695"
     CDS             complement(7711..8538)
                     /locus_tag="ACHGMT_001695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN6835437.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            complement(8538..9338)
                     /locus_tag="ACHGMT_001696"
     CDS             complement(8538..9338)
                     /locus_tag="ACHGMT_001696"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN6835438.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            complement(9331..10470)
                     /locus_tag="ACHGMT_001697"
     CDS             complement(9331..10470)
                     /locus_tag="ACHGMT_001697"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFN6835439.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            complement(10675..11868)
                     /locus_tag="ACHGMT_001698"
     CDS             complement(10675..11868)
                     /locus_tag="ACHGMT_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6835440.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(11947..12153)
                     /locus_tag="ACHGMT_001699"
     CDS             complement(11947..12153)
                     /locus_tag="ACHGMT_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFN6835441.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            complement(12146..13288)
                     /locus_tag="ACHGMT_001700"
     CDS             complement(12146..13288)
                     /locus_tag="ACHGMT_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFN6835442.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            13800..14435
                     /locus_tag="ACHGMT_001701"
     CDS             13800..14435
                     /locus_tag="ACHGMT_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN6835443.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            14571..14663
                     /locus_tag="ACHGMT_001702"
                     /pseudo
     CDS             14571..14663
                     /locus_tag="ACHGMT_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            14777..15412
                     /locus_tag="ACHGMT_001703"
     CDS             14777..15412
                     /locus_tag="ACHGMT_001703"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN6835444.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            15519..16757
                     /gene="mef(H)"
                     /locus_tag="ACHGMT_001704"
                     /gene_synonym="mefH"
     CDS             15519..16757
                     /gene="mef(H)"
                     /locus_tag="ACHGMT_001704"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFN6835445.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            16984..17403
                     /locus_tag="ACHGMT_001705"
     CDS             16984..17403
                     /locus_tag="ACHGMT_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFN6835446.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            17408..17647
                     /locus_tag="ACHGMT_001706"
     CDS             17408..17647
                     /locus_tag="ACHGMT_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6835447.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
BASE COUNT         5450 a         3533 c         3141 g         5654 t
ORIGIN      
        1 aataaaccaa aaaaggctgc gacggttgtc acagcctctc tcgtattttg tgcctatcaa
       61 tgatagcgtt ggatcctgga agaatgagcg ctatctggtt gttcctcata agatgtatca
      121 tcataagtca tatcgtcata tgcctcttcc ataacataga attcctccgc aacttctggt
      181 tcatcatatc gatgaacggc catagccaac aggatataaa taatcggaac ccatgggtat
      241 gaagtcaatg tatcctccat aaatgactgc atcatcaccg caaaaaaggc ccagataaaa
      301 gtcatctgcc cgttggataa atctgctttc aacgttaagc gaccaataaa aaggataagc
      361 agcaaagcag caatcccaaa tcctatcaat ccatagatat tcaaaataga attataacta
      421 ttgtggtttc ctaaaaattg gtttcctcgt tggaagataa atgttttcat tcctagtaaa
      481 atttgttcag atttttctcc tagcgtctga tagaatttat gccaaatatc ttctcgaccg
      541 gtaaacaaat tgactttttc agagacagcc gccagataag aaatcggcaa agctaaaaga
      601 atcgtaaccg tgtaagcaat aaatggcgca cgaatgacac gcatcaacga tttaggtaaa
      661 agatccaata tcgcaaaaac aatcaacgcg actaatgcac ctctagattg gcagacccaa
      721 atcccgagaa aagctgcaac aactgctact gcaccgatga ttcgtacata ccatcgttca
      781 aaagaactgg caaatccact gatcaataaa gcaagagtca tcaaagaact tccgattata
      841 ttcgtattga tccaaatatc agaaagcgaa ttgcctggag cccagatgct ttgtccttct
      901 ggtacttgga tctctgtaaa tacacgatat aacgtgattc cgaggaagac cgaaaagaca
      961 atcagataaa taccgcgatc ccattttgtc cactcgatcc atttaattac taaacaaaat
     1021 aaaataatca aaggaaacag taagtctcca aataaagact catttcttaa ttcccgaaag
     1081 taagaaaaga aaatgaaaag ggcaagcgca acacctagat aaagaaaatc gacgcctttt
     1141 gctttataaa catttaaaat gagcaaaata gccacactaa aagcgataag taatgaacta
     1201 ttgttatata taaacaaagt tgctggaaaa cctaaccctg ctgtccaaac aaaaaagact
     1261 agcgtcagta acaaaagcaa gaaaccgaca ctcattttgt tgacaaattt ttgtacgatt
     1321 gcttgaatta tctccactct cctttctact aacatttcag gattattctc agctagccta
     1381 ttactgtacg actagctgat tttcctgagt ttgtgtttgt ggtacatctg gataatccgg
     1441 atattctact ttaaaaatca tataaccgtc tttatccggt ccatatagct gcgtgaactg
     1501 cgcttcatat aattggttgt aatcagttaa tggtcgttta ctgatcacat agtcgatccc
     1561 taatttttct gcgtcttcaa aattcaacgt gaccgaaaac atatctggac gatctaaaat
     1621 gaactttgta tcttccttag cgataaaagc tcttgtatga gcataccgat tataatactt
     1681 ctcgtattct tctttcggat cgattttctt ccatgcagct atatctggat aaaagcgtac
     1741 tgtattgaag gatttcactc ccaatgttgg ggtgaagtta tataattcat cttcagacaa
     1801 ccaaacttta ccaggatctt gttgatcgat tttttggatt tcttttgcca gtgttttttc
     1861 aaaaatcgct cctgtacctt ctacgactgg attgacgaaa aagccagata cgataactaa
     1921 ggcggcaaaa gcgaggtaaa acaagcgtct ccagccaaat aatgcaaaag ctaaaatcac
     1981 tgttcccaac acagcaactg cgatggtttc aaaatccgaa aaataattat ccattttcga
     2041 atgcgtaatc gcatagaaat aagaccatgc atttactgcc aacaaaatga atttatacca
     2101 aataggaata acatcttctc tccaaatata agcgataaac cagatcgata ataaacaagc
     2161 cgcaaaacta aaagtaagga tggctcttaa tggaggaaca taagacagca gagtgatttc
     2221 tgctagccat tttggcaacc caacggtaat ccaaaagata gaaaacagac aaaaaatcat
     2281 caaaatacgt cccagtaact tttgttcgct atcttttttg ccaaataaca caaatggact
     2341 tgctaggaat acagttggaa aaaagtgata gaacagggcc acttcagagt tattcgaata
     2401 gttgatatct tcaaaaggaa ttttccaatt tgtaaggaaa gaaaaaaatt cgccaatcgt
     2461 ccactttcct ccagtactta cccgttttcc tggatataag gtattcaaag aagctaacaa
     2521 tgcgtctttt gacgttagcc agaaatgaac catgactccg ccaataaata aaactgcccc
     2581 tccaatcaac actacatcca acagatccca tgaaactttc ttgctatagt aaataagcag
     2641 gccaacaaaa taaaaaatca gcaagtacgc cagcatcact tgatgggctg ggtaaatcac
     2701 taaaataaac ccaataccga aaataaccac caaaagcatc atcaacgttc gtagccattt
     2761 tttatcatga ttgtagaaat aatgataaaa agcagtcatc agtcccaaag taaagaaaat
     2821 caaatcgccc acatgttgca tgaaccacca ctggactgct ggagaaaatg tcagcgcaaa
     2881 tgcaccgatt agcgatagag ctttttttct cttcgttaat atcatcagta cttcgaatcc
     2941 aagcaagacc atgacgacta atttcatcgc ccaataaaag gaaagtcctc gatctcttcc
     3001 gaggaaaaag aatccccagt tgaatggttt tccgataacc gtaatatcct ttacaggaga
     3061 attatacgca atgatcatgt tttgtccgtt taatgaataa tcctcgttca ccaacggaaa
     3121 gtcctcttca gcttgtgaga ggtaaaaagg tgtctgcacc agccattcgt ctgaccgcac
     3181 ttcccgattc tgtccaaaaa tcacatcagt ttttttccca tcgtctcttt gcgacacata
     3241 tttatcccaa acaccaagtg aacttccgtt taaattaaaa gtaacgatga ccacgatgac
     3301 taaaacgcca ataagatagc ggagttttag aaacaggtca agcaccaaat tcgaatctgc
     3361 ctggttcgct tttggctttt cttttcgtcg tgcacttctt gtttgttttt ccatatcatg
     3421 ctccaaattc tatttgataa aaggaaatta cttattcatt tttttggcag cttccagcat
     3481 ttgcgcaagt gcttcttgcc aagttggtat gacaaatcct aactcttctg tctttttcag
     3541 gctcatgact gaatactgcg gacgtgttgc tttttgcgga aattcttctg acgtaacagg
     3601 tgcaacttct acatctgtat ctttcaatat ttcttttgcg aattcatacc aagaacagct
     3661 attttcgttc gacaaatgat aaataccaaa tggtgctttt tcttcgatga catataccat
     3721 aaattctgcc aaagtacgtg tccaagttgg tcgcccaaac tggtcattta ctacagtcag
     3781 tttgtcgcgg gtttccgcta ggcgttgcat cgtgaagacg aaattgtgtc catagatccc
     3841 gaaaacccaa gaagttcgta tgatataata gtcttctaaa atctcttcta ctgctttttc
     3901 gcctaataat ttcgtacgac catattcatt tagtggattc gtttggtcgt ctacttcgta
     3961 ttctacattt ttgttttttc catcgaaaac atagtctgta ctgatataga cgaattgagc
     4021 attcacagct tttgctgcaa gtgctacatt tcttgtacca tcaacattga tcttctcatc
     4081 taattctttt gcttcttctt ctgctttatc tactgctgtg taagctgcac aatggaaaat
     4141 aacatcaggt tttaattctg tgatgtaagc cattgttttc tcggcatctg tgatatccaa
     4201 gtcttttgca tctgttgaaa catattgcag atttttttca tctaataaat ggcgcaattc
     4261 tgtacctagt tgcccatttc ctccagttaa taaaaacata tgattatccg ctccttgatt
     4321 tttattttaa aaaagagaca ggcgagcgcc tgcctcacat gtgtttttcc attaatacag
     4381 tgacattttc tctgtattat tgtccatttt tggcatatgc tgcttcaacg gcctcttttt
     4441 cttttttcca ccagtcttca tgttcttcgt accatttgat cgtgtcagcc aacccatcac
     4501 ggaagttcgt aaactcaggc ttccatccta actcttcccg taattttgtc gaatcgattg
     4561 cataacgcaa gtcgtgtcct gcacggtcgt ttacatggtc gtacgcatct tttggttgac
     4621 ccatcatttc taagatcaat tccatcacgg tcttattatc ttcttcaccg tctgcaccga
     4681 tcagataggt ttcgccaatt ctgcctttcg tcaaaatcag ccacactgct gaagaatgat
     4741 cattcgtatg gatccagtca cgaacattct ttccttcgcc atataatttt ggacgaatac
     4801 cgcttaagat attcgttact tggcgaggaa taaatttttc aatatgctga taaggaccat
     4861 agttgtttga acagtttgaa atggttgcac gtaagccaaa agaacgcacc catgctttta
     4921 ctaacagatc tgaaccagct tttgtagaag aatatggact tgaaggatta tatggtgttt
     4981 ccgcagtgaa tttttctcct tcgccttccc catgtcctgg caggtcttca cgtaatggaa
     5041 gatcgccgta aacctcgtca gtagatacgt gatgatagcg tacatcatac ttgcggcaag
     5101 cttctaaaag tgtatacgta ccaatgatat tcgtttgtac aaatgggaaa ggatctttta
     5161 atgaattatc gttatgggat tctgccgcgt aatgaacaac tgcgtccgct tcttttacta
     5221 gacggtccac taattctgca tctgcaacat ctcctacgac caattctacg cgatcgctag
     5281 gaagtccagc caagttctct ttgtttcctg cataagtcag tttatccaaa acagtgacat
     5341 gcacttctgg atgattgttt accacgtaat gaacgaaatt agagccgatg aatccggcac
     5401 ctccggtaac aatgatgttt ttcataagaa aatttctcct taattcttag atttctccgt
     5461 aaacaaatgg attttctgct tcaaattctt ttaatgtcgg atgacggtga tctttttccg
     5521 acataatcgc tcttgatgga tcaattggcc aatcaatcgc taaatctgga tcatcaaatg
     5581 cgatgccccc atctgcttct gcattatagt aattatcaca cttataaagg aagttcacat
     5641 ttggtgtcaa agtaacgaat ccatgggcat atcctttagg tacaagcaac tgtcgatgat
     5701 tatgttcaga taagataaat ccgtcccatt gaccataagt cgggctacct ttgcggatat
     5761 ccactactac atctaaaact gctccagtga ctacccgaat caattttgtc tgtgcagctt
     5821 ctcctttttg gaaatgaagc ccccgaagga caccagcttc agctgatagt gaatggttgt
     5881 cttgaacaaa gtcatatttc aatccatgtt ctgcaaattt tgcttttgaa taactttctg
     5941 tgaagaaacc gcgatgatcg ccaaagacgt ccatttctac gattttgaca tctgttaatt
     6001 ttgtatctgt tactttcatc tgtaaggact ccttattcag cggccaaatt caataaatat
     6061 tgaccgtatt gatttttttt caatggctgt gcaagggcca gcaattgttc cttatcgatg
     6121 tagcccatac ggtaagcaat ttcttccaaa caagccactt ttaagttctg acgtttttca
     6181 attgtttcga taaaggtaga tgcttcaagc aatgattcat gtgttcctgt atcaagccaa
     6241 gcaaatccgc gtcccataag ttctacggat agttttcctt tatccaaata tgctttattg
     6301 acatccgtaa tctctagttc tccacgttct gaaggtttga tacctttagc gatagaaatt
     6361 acctcattgt cataaaaata aaggccagtt acagcgtaat ttgattttgg ctgagcaggc
     6421 ttttcttcaa tcgacaaagc ttgcatattc tcatcaaatt ctactacacc aaagcgttct
     6481 ggatcattga catgatagcc aaatactgtc gcgcctgtat cttttgaagc tgctcgttgc
     6541 aacattttag acagaccgcc accatagtaa atattgtccc ctaataccaa gcagacacta
     6601 tcatctccaa taaattcttc tccaatgata aacgcttgtg ccaatccatc tggactttct
     6661 tgtactgcat attcgatctg cagtcctaaa tcatttccgt ttccaaataa ttccttgaaa
     6721 cgtggtgtat cttgtggcgt tgaaataatc agaatttctt tgattcccgc caacatcaga
     6781 gtagacattg gataataaat cattggtttg tcataaatcg gcatcaattg tttcgatgtt
     6841 gcctttgtta aagggtacag acgtgttccg ctgcccccag caagaatgat tcccttcatg
     6901 gatatctgct ctcctttttg atttcaaatt tcactttgtt attatctcat caattgttat
     6961 atttgtcatg gaaatattaa ggatttaaac atcttttatc aacgtttaaa ccattttcgt
     7021 ttgatcaacg ccgtaatcag atgaacacgc caagaaattt tttgccgtaa tttagaatgt
     7081 gtttcaatct cactcgcgtt taaatcatgt cttcggtaca gagttaacgg ttctcttagg
     7141 aacccagtct tctttcctcc cagcaaacca atccacatat catgcatcgg cacttctgac
     7201 gggatcggca aaatatcggg ttttaaagaa gaccggaaag ccataccagc accaatatag
     7261 tgacttctta tcacattatt aagccatcct tctttggaat ttcgaaacga aaaatacgaa
     7321 tcatggatct cttgcagctc gctgtccaca atgattaaat cagaaactac tactagctga
     7381 tccggattct gctcaaaata gtcagtgatc tttttcactt ttgtctccaa ccaaacatca
     7441 tcttggtctg ccaaaaagat tagatctcct ttcgtctgct ggatcgcata accaaaatta
     7501 gcgatcaatc cttgacgagg ccccgaaaaa agctggattc gcttatcttt ttctgcgtat
     7561 tcagttaata tatcccatgt atcatctact gacccatcat cagaaatgac tacttcatct
     7621 tctgctgaaa gctgagataa aatactatct aactgctctc ttaaatatct gcctccatta
     7681 aaggtagcta tacaaaccga acgcacgaat tcacaccttc caattcttaa catatgagcg
     7741 caaagtcatc cgccagatat gccggttttt gacggtcaag aactgtttag ctgttcttaa
     7801 caataattgt cgtcggtgac tggataaatg cctttgatca tattcatgat aaaatcgatt
     7861 ttctgcttgt aaaatagaac ggtatcgatt acttgaaact ttttcatagt ctaaaaccga
     7921 aagatcatgt tccattcgta caggaaggat ctccacagtt ttttccaact gatccacttt
     7981 ccaaaacagc caatgatcca aaaaatctaa agaaaaagca ggattaaacc cgccaatctt
     8041 ttttagaaat ttgacagacc aggcaacacc tgaattgatt gccatgattc gttcagctgt
     8101 tgggccttca acttctactg cttgagctgt acgattgata taacgatctg aatatagggg
     8161 agaaacttgt cggctcccag aaaaaatcat cggaactgca gcaacacatt catctgtaaa
     8221 ggttacttta cgcaatagtt caaaataatc gtcgactagc ctcgtatcct gatccagagt
     8281 gacgagacaa cgtgttcctt catctgcttg agacaacgca aaattataag ccaccgctaa
     8341 accaggattt tgcggatcat gatgataaac gatattttct ttttcaaaca gctcattttg
     8401 ctgcctttga ggactattat cgtaaataat aagctgaatt tgcttttcgc taagtgcttt
     8461 gcttaatggt ccataagctg gagattgact gaatagttgc tgataaagca caacgactac
     8521 taatatttct ggtttcatca gaaaaaacgc cacccccact tattgaaaaa tttcatcata
     8581 gaagtcaaaa acacgcgaaa cagttttgca tttcgatgcg cacccttccc gtaaaaatgg
     8641 ataactgcat aaaagggtgt gtacaataac ttgtaacctg ctttttcaaa actcaaacac
     8701 aagtcgttgt cttcaaagta catgaagaaa cgctcatcaa acccctttat ctctttaaat
     8761 ttatcaatat cagctaccat aaaactacct gagatcatcc gtacataact attagcatca
     8821 gtaggcaatc ctcggcattc aaacacagcc agtcgtttgt caaacttttt tttgacccat
     8881 ttgaaaggca caaagcgcaa aacgtaatca aaaacagcca gctgctcacg gatgaggtgc
     8941 tgagttgttc catcttcatt aagaatctta ggtgctaaca tcgcacattc cggatgttct
     9001 ttcaataaca agaccaatgc tgtaactgtc tcttcatcca ctaaaatatc tgggttacaa
     9061 ataatgccat agcgagaatc gctagctatc agattgtgat tatgtccata accaaatccg
     9121 gaattttcat catgaaaata gatatcgatg tagggatatt tcctatactc tgttagtctt
     9181 tttttgtact cgggaagtga attgttgtca aaaattcgga tttttactgc tgtttcatct
     9241 tttacagttg aaataaaatt atctagtgtc cgaaaaattt tatcactgtt tgccgtcaca
     9301 atactaataa ggatcggctg gtttaatttg ttattcatct atatgacggt tccatttctt
     9361 caaatatttc tgttttactt gcgtacggta ctctttattc cctaaaaatc gcaatgtttt
     9421 taaaataaac tgatgtttct cattgatcag accaatcaat tcaaataata aaattgcagc
     9481 aaacaacatg gcaataatca atatgatcgt tccgattctt ccagtataat tcatgaccat
     9541 agctgtgaaa gaaaacatca aagccatcgc ataaatcgtc aggacagcac ctttatgagt
     9601 aaagcctaaa ctgattagtc ggtgatgaag atgtttttta tctgctgtag aaaaagaaac
     9661 acggtttgct tttctcctaa tgatagcaaa aaacgtgtct gttaccggca ctcccatgac
     9721 gatcagcaaa gaaatcgatg aaacaaaagt cacgttcttc agcccttgta gtgaaaggac
     9781 ggccatcata aaccctagga atagagctcc tgtatcgcct aaataaatct ttgccggata
     9841 aaaattgtaa ggaaaaaaac cgataatact tgctacaaga cagaaaataa caactggaat
     9901 atacactgta gaagcatgga ggaaaaaata tccaatgatt cctatcgtaa ttaaaccgat
     9961 caatgaaatc cctgccgcca gcccatccaa accgtcaatc agattgaccg cattcgtgat
    10021 accgaagatc caaaaaatcg ttacgggaaa actaagccag cctagttgga tatatccaat
    10081 aaaaggaagc gtgaaggaat caatacgtat atctgctaca aaataaataa taagagaagc
    10141 aatcaagatc cctatacttt tttgtttagg tgtcagctca tatttatcat cgagcactcc
    10201 tgtgactatg attactaaag aagcaatcaa gatcggtaca atatacgatt gagggataat
    10261 tttacggaat aagaataaag tcgatataac aaatgaaata aagataggca gtccgcccgc
    10321 tgaaggcatt ggaactttat ttattcttct ttcatttggt ttgtcgacca tacctgtttg
    10381 tactgaaatg attttgaata caggtgttaa tatcagtgat aataaaattg tcagaaaaaa
    10441 tcttataacc atactaaaag aaacaagcat atgggcacct actttaatta agagtcataa
    10501 ttattataaa ttatatatgt ctttttgact atctttttat atcttctcaa atgtccgcaa
    10561 agccttattt tatcggctct acgggcattt tgcttttgtg gtaaacctcg catatctagg
    10621 tctatcttct tatattttcc ctgtcaatcg tggttaaaat cgtggtaaat acttctatgc
    10681 gattagacgc tgaacctctg attttgcggt atctatggac gcatgagcgt accagttcat
    10741 tgtgatactg atgtttgaat gccccatgat atactgtaaa tctttcgggt tcatgttctt
    10801 gcttgccagc cttgtgcaga atgtatggcg tagcgtatgc ggtgtaatat gtggcaaggg
    10861 attgtccttg tggtgcttgt tgtatttctt taccatacgg ataaataagg cgttataatc
    10921 aatggcaact tttggattac ctttcggatt gataaacaca aaaccgctgt gtccgtctac
    10981 ctctatcgac tttatctttg gtcggctgtt gataacccgt tgaaatgcct gtatcgtttc
    11041 tttgcttaat ggcacttgcc ttatcccgct tttcgtctta ggcgtttcaa tataatagcc
    11101 ctgttcctta ctctttagta actggtggtc gataatcaca acttcattct tgaaatcaat
    11161 gtcggctact gtcagtccgc acagttccga gatacgaagt cctgtcttta acagtatcag
    11221 cacatcatca taatgcttgt gatacacgtt gtccgtctta ataaatgaca ataaggcttg
    11281 ctcctgttcc tctgtcaatg cgactttctc tttggtatca ttttctatga cttcacttag
    11341 cttgaaatca aaagggtttt tcctcacata atcatcttgt atggctatgt agaatgacgc
    11401 ttttaacgag cgtttatggt tgttaatggt attgtaggaa aagcctttgt ctttcatgcg
    11461 taacgcccat tctttagcgt cagagggttt tatcgtatca atgctcctag cacctaactt
    11521 gtcctctttt aataaccgca tgagttgttc ccgttgtttc tgtgtgcttt tcttcacatt
    11581 tgtcctctgt gcgttctgtt tggcatagag ttggcaaagc gtcattttct tgcctgtgct
    11641 gtcgataccg tcctcaatat cccgtcttat ctgctgttcc agttctcgaa gtgagggttt
    11701 ttcccgtttt ccctttggtg tcgggtctgt gggtgtcaat ctccaagcat acacatattt
    11761 tgtgtttccc aatgcgtcta catatttata taagtatttt ccgtctgttc gttggctctc
    11821 tccagtatgc aggatacgac ctttgttatc ccgtcttttt tctttcatgg tgtctgctcc
    11881 tttccttgtt ggaaagagcc ttgatatgac ttgtgttcat cataacacat acaaggctca
    11941 tttgcattag attgcgtcca atttatcaat aaccttttca aactgtcggc gtttaatctg
    12001 tatgcggttg ccattcataa tgagccagcc agcgtccttg ttttcctctg ccaatcgtct
    12061 taacttgttt tctccaatgc ggaaatactt tgacgcttct tcaatggtaa gggtgtattt
    12121 ttcccatacg ggaatatcct gtgtattact cataaaacac cccctcactt tcgattacgt
    12181 ctgatatgtc cgtacacatc tgttccacaa gcatttcctg tttttcagaa aggtgggtac
    12241 attcatcaat cattttcatg tagtttgaac ctgtcagcct gtcgatttcc tctatcatca
    12301 cgattgacgg catgacctgt ctttccagcc attgcagagt gcgttccata ttgacaggta
    12361 cagggttcat ttcaaaaggt aaactgtcat gggaaatgaa tatctcccac aatggatagt
    12421 caggaaattc cacaaaatcg gtaatgagat aaaacaccag cccatgaggg ttatacgtca
    12481 ataacagttc ttcaactgcc tgtaccgcct ttttgtcacg cagacgaatt tcaaaacggt
    12541 tgataatatc ggtatgtttc tttttcgttg cctgttcctt ttgtttttca tacaggcaga
    12601 aatattttgt gcttgccttt gagccgatat aaagggtact tgctaaatta cggttttttc
    12661 cgcttaattt tccaccttgt acattttcat agtttttaca gcgacattcc gcaccaccat
    12721 ttctgtattt ttcagatagc acaggaatat ccagcagacc gcacatatca ttgattgcca
    12781 agtcaaaacg tcggataaca ccgccacagt caagacagcg gtttaagaat gaataccagt
    12841 ccctgttctg tgcctgtaag acatattcca tgttgcgtga gcctgcccct tttaattcta
    12901 aaaatacgcc catgtgttca tcatcagaag ccatgacctt gatttccccg taagcataat
    12961 gctccttata gccataatac ccatagtcat aatggataaa ataatctgct ttcatgccaa
    13021 gcacctttcg gataacttcc aatgcgtcag tcgttggaaa acggacagac agataatcaa
    13081 cgagcagata aaaaggctta tcgcagatat agtaattcag acaacgcagg ataatgttct
    13141 gtgtttcctt gctggcgtta gcttttccgt tctcaaagcg gttgatacta tatcttgata
    13201 catgggtaag acttgcaagt tctccttgtg aaagaccgct ggcaagacgt ttgttcttca
    13261 tttcaagtgc aaattctaaa ttgttcattg tattcctcca atcccacaat agccttgaaa
    13321 tacctgtaaa ccacctttca aaaagtgtga tatgtactat cttcttaaaa cctctcatta
    13381 ctactcacag gtttgggggt gtatggtttt tgacttgtct tttgtgaaat agtgcccccc
    13441 tgttagataa cgggggcaca gacaaggctc gcaggctcgc cagccgacaa agcatttctt
    13501 ccccttgaca caaggtgtca tggcggtaag cctgagaggc ttccgccacc gtgtcagaaa
    13561 tactttgtca gcaagttctg ttttgccttt tctaacaagt tccatttcaa ccgatattca
    13621 tatctacgga tttaatcaga taggtatttc tttttgaatg tttttatatt tcattctatt
    13681 gtgcttattc gattacaaga acatagtaat tcttatatgc ttatttgtca atatcttttt
    13741 ttgcctttac aaatcatatt aagcatgata gacttataat agcgaagata taacgagata
    13801 tggagggtta tgcaatgaaa ataagccatt taggaaataa tatacagacc ataagaaaat
    13861 ttaggggaat gaaacaacag gaacttgcgg acaaaatcgg tatcaatatg cagagccttt
    13921 ccaagattga aagagggttg aactatcctg cctatgaaac gctggaaaag ataatggaag
    13981 tgctggacgt tacgcccaat gaattgttgt cgggagaatg gaagtatgtc aatcaatctg
    14041 aaaaggaagt ctgtcagttt ttaagagccg aagaacgtct aaatgcagaa ctgaaacatg
    14101 gacactatga taacttcttt gacagcgagg aagaatggct ggaatatgag ttagaaaagt
    14161 tacgggaata tattacagac tatatcaacg ggaaaagcat tgaagcgtcc gacctttacc
    14221 caatcaaaga atttatccag catttgaagt ttcaaaaatt attagaccgc tatgatgatt
    14281 tatacagtat ggatatgttt ggggaaagca tagagggaca taagtataag acaccttatc
    14341 aagtcgtgaa aatgataaac ccgaactcaa aagaagatat ggaactgtta cgagaagtat
    14401 taaggaataa caattttgat gatgaggacg agtaactttt ttcctcttgc gagaagatac
    14461 agcaaacaaa aagcccgatc aagagtgcaa agcaccacca atggtggcaa agctcttgac
    14521 agggcttttt cttttctgtt gtgggcaatc aagaggaaga aagggataaa atctgatttg
    14581 tggtaaatca ggcaaaggca acgcaggtat cacagtatga attgacaccg cataaggaaa
    14641 gcaactggaa gattgtagaa taaatgaaaa aggcttgcat tctgcatttt tataaaattg
    14701 caagtcttta tattgacaag cataaaatac taagatatac tttaatcaga ccaacggttt
    14761 gtattggagg taagttatgg cacgaaataa atatccagaa gaaacagtaa aactcatttt
    14821 agatgtttct acaaaaatgt tttgtgaaaa aggatatgat aacacatcat tgcaagatat
    14881 tatcaatgaa acaaaacttt ctaaaggtgc tatttatcat catttcgatt caaaggaaga
    14941 tattttaaaa gcaatttttc aaagaattgg aaatgaaaat gcagtaatat ttgctaaaat
    15001 tagagataac gatagtttaa acggtttaga aaagttaaga gaaatattta agacagcggt
    15061 attcaattct aaccaaagcg ttttattgac agtttctcct caactattaa acaatcctcg
    15121 ttttcttgct atgcaaatag aacaaatgta tgaaattgta gtacctgaat ttgttgaacc
    15181 cgttttagaa caaggtatca aggataagtc tttggagatt gaaaattcac atgaaattgc
    15241 agaagttatc atggtattaa gtaatgtatg gctcaaccca ttagttgaaa tgacggataa
    15301 agagggtatg gaaaaacgtt gcaatacatt caatgccctt ttaaatggaa taggcataga
    15361 cacattatta gacgaagaaa caatttctgc atttatacag ttttgtaaat aactgtattt
    15421 gttatcccta tacgatagac tgctctataa aagagcagtt tatttaaacc attatataaa
    15481 ccgacggtct gtttgattaa ataaaaagga gagattttat gcaagcagaa aaaaaacttt
    15541 ttaatcgtga ttttatactt gttgttatag gacagataat ttctttattt ggaaacgcta
    15601 tcctacgatt tgcattaccg tactatttgt taaaagaaac tggttcatca gctttgtttg
    15661 gtattgctac agcctgctct tttttaccta tggttatttt gtcgctggta ggtggtatta
    15721 ttgctgacag gacaaataaa agaaacatta tggtcatact agattttgta acagcaatca
    15781 taattttagt attttatttg tcattgggaa aaattcctat catacctttg tttattgttg
    15841 ctttgatgtt actttatggt atttctggtg catatcaacc agcggtacaa gcaagtattc
    15901 ctctattggt atcatcagat aaacttaccg tcggtaatgc tataataaat caagtaaata
    15961 ctttagcaaa ctttatcgga ccagctataa gcggagtagt ttttcaattt tatggtatca
    16021 atcccatttt aattgttagt attatatgtt ttactttttc agccattatg gaaattttta
    16081 tccatattcc acaccaaaaa agacctcgtg aagtaggtgt aattgcagtt gctaaaaacg
    16141 atttaaaaga tagttatacc tttttgaaaa aggagaaacc catttttata tccgtaatct
    16201 ttttggtttg cattttcaat ttggttttaa gtgctgtttt aattgttggt ctgcctatct
    16261 taatttctga agttttaaaa atgccagata cactatatgg tttttcacaa tcagcattat
    16321 cattaggtgg tttatgtggt ggtattttaa cagctcttgt tgcttcaaag ttaaaactaa
    16381 aaaaccttta taccttgtta cttatttgtt ctgtttctgt tttacttatg ggaatttcgc
    16441 tatttttaga attgcctgca atagtttcct actgggtagt tacagtgatg tgctttgtgg
    16501 ctatgggtgt atctactatg tttgttgttc aaatttacac tcttgttcag acacaaacac
    16561 caccgcaatt agtaggaaag ataatggcta cgctggtagc agttgctatg tgcggtcaac
    16621 ctattggaca aatggtttac ggtattttat tcgatgtatt tatacaagaa acatgggttg
    16681 ttatgtttgt agcttctatt gcttcaatca tgatttcaat atattcaaaa agaatttttc
    16741 aaaagttaga gatataatac aaggggttaa atagtatgtt aggctaaaaa caatatataa
    16801 aagaaaattt gaaatctgct aaaaggatag tgttataaaa gaaagagtaa tattgcagta
    16861 tttaaggctc gcacaaatat atgttttgtg tgggcttttt tcttaccatt tttctgtatt
    16921 tctaaagggt ataaacggta ttccgtttat atatttcagc attagcagaa agggggtgag
    16981 attatgagga catcttcttt cgagcatatc gtcagattac agttcaattc tttaatgctg
    17041 attgtcatta agaacaaagt gaaaagtcga aacagacagc ttgcaagaca ttctaaacgt
    17101 gaagtattgt tttgtgaaat agccgaaacg aaacaagttg aacgtggaac gattgatacc
    17161 tgtttttatg attgtgtatc atttaaagtc ttgcacttta caatttatgt atctgatgaa
    17221 acacttggta cagttttaca tagactgtca gaaaaacagc gtagtgcgat tttattacgt
    17281 tattttcaag gaatgaacga cagagaaatt tcagaactgt accatgtatc acgttctgct
    17341 atttgcagta gaagaactag aggactaagg aagttaaaaa tgcttttgag cgaaaggaag
    17401 taaagaaatg agtaaagatt atggctatcc ctcttttgaa ttgatttgca aagcgtcaag
    17461 cggagatgaa atggcagtta aagagatttt gaagttttat gacggttata tttctaaatt
    17521 atgcttgcgt cctttttacc actctgaaag tggtaaaatc attatgcagg ttgatgaaga
    17581 actaaaagga gaaattcata cagacatgat gaaagcaata ttgaaatttg aaatcggagt
    17641 gaagtaatca actgtataaa aaaatcggga gatacacagt ttcgtgctat ctcccttagt
    17701 agttttacgg tcatatcaag gctcattcaa tcgtggtaaa ttcgtggtaa gatgagtgtt
    17761 tttctatact tcaaaatg
//
LOCUS       DAZBSA010000054        17740 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000054 DAZBSA010000000
VERSION     DAZBSA010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.054"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..841
                     /locus_tag="ACHGMT_001707"
     CDS             29..841
                     /locus_tag="ACHGMT_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFN6835448.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            838..1638
                     /locus_tag="ACHGMT_001708"
     CDS             838..1638
                     /locus_tag="ACHGMT_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFN6835449.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1799..3067
                     /gene="rseP"
                     /locus_tag="ACHGMT_001709"
     CDS             1799..3067
                     /gene="rseP"
                     /locus_tag="ACHGMT_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFN6835450.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3135..4844
                     /locus_tag="ACHGMT_001710"
     CDS             3135..4844
                     /locus_tag="ACHGMT_001710"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFN6835451.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5056..9408
                     /locus_tag="ACHGMT_001711"
     CDS             5056..9408
                     /locus_tag="ACHGMT_001711"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFN6835452.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9552..10025
                     /gene="rimP"
                     /locus_tag="ACHGMT_001712"
     CDS             9552..10025
                     /gene="rimP"
                     /locus_tag="ACHGMT_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFN6835453.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10049..11224
                     /gene="nusA"
                     /locus_tag="ACHGMT_001713"
     CDS             10049..11224
                     /gene="nusA"
                     /locus_tag="ACHGMT_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFN6835454.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11246..11539
                     /locus_tag="ACHGMT_001714"
     CDS             11246..11539
                     /locus_tag="ACHGMT_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFN6835455.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11536..11847
                     /locus_tag="ACHGMT_001715"
     CDS             11536..11847
                     /locus_tag="ACHGMT_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFN6835456.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11860..14166
                     /gene="infB"
                     /locus_tag="ACHGMT_001716"
     CDS             11860..14166
                     /gene="infB"
                     /locus_tag="ACHGMT_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFN6835457.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14193..14540
                     /gene="rbfA"
                     /locus_tag="ACHGMT_001717"
     CDS             14193..14540
                     /gene="rbfA"
                     /locus_tag="ACHGMT_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFN6835458.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14648..15152)
                     /locus_tag="ACHGMT_001718"
                     /pseudo
     CDS             complement(14648..15152)
                     /locus_tag="ACHGMT_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15317..17680
                     /locus_tag="ACHGMT_001719"
     CDS             15317..17680
                     /locus_tag="ACHGMT_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HFN6835459.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT         6048 a         3148 c         3908 g         4636 t
ORIGIN      
        1 gaatctttta agtataggag gaaaaagaat gttacgtttt tttccgcaaa aaaacaaata
       61 tattcaagaa aaaagcgaat ttcaatttaa taccgatgga ccaattcctc agcacattgc
      121 catcatcatg gacggcaatg gtcgttgggc gcaaaaccgt cgtttgccac gagttgctgg
      181 ccataaagaa ggcatggaaa cagtaaagaa agtcacaaaa aaagcatcaa ggcttggagt
      241 gaaagtgttg acgctttatg ccttttcgac tgaaaactgg aagcgaccaa aagatgaagt
      301 aagttttctc atgcaactac ctgtcgactt ctttgatacg ttcgtaccag aattgattaa
      361 agaaaatgtc aaagttcatg tcatgggtta tgaaaacgta ttgccagaac atacgcaaga
      421 tgctgtaaga cgtgcaattg agcaaacaaa aaacaatact ggaatggtat tgaattttgc
      481 tttgaattat ggaagtagag cagagatcgt gactgctgtc caagaaattg ccgaagaagt
      541 agcaaaagga gaaatacacg cagaagaaat cgatgatgaa ttgattgcta agcatttaat
      601 gacaggtttc ttgcctaaag agttacaaga tcctgagctg atgatccgta caagtggaga
      661 agagcgaatt agtaattttc tgttatggca gatcgcatat agtgagctgt attttacaaa
      721 agcgctttgg cctgattttg atggtgcgca tcttgaagaa gcaatcgcct cttatcaaaa
      781 tcgagaccgt cgattcggcg gtgtaaaaaa aacagaagat gagaaagggg atcagtcatg
      841 aaacaacgtg tgatcacagc agctgtggcg ctagtcttgt ttattccgat tatatggatc
      901 ggaggcatag cggttgagct tacagcagca atcttagcgg tagtaggagt ttacgagctg
      961 ttccggatga aaggattgac actcttaagt tttgaaggag ttttatccgc cgttggtgca
     1021 gtgttacttg ttttgccaaa agaaagatgg tttttctttc ttccagagaa tgtaagtaca
     1081 tttatgttat tttatcttat agttatgatt cttttaggaa catcagttat ttctaaaaat
     1141 acgtatacga ttgatgaagc aggttttcct gtgcttgtca gcttgtatgt gggaataggt
     1201 ttccagaatt ttgtcaatgc acgaacagaa agcttggctg ttttgatatt tggtctattt
     1261 attgtttggg caactgatat cggagcatat atggttggtc gacgttatgg tcagcataag
     1321 ttaagtcctg atatttcgcc caacaaaacg attgaaggtg cgctcggtgg tatcggtagt
     1381 gctgttgtag tcgcatttct ttatttattg ctatacccag caaaagaact attcaatcat
     1441 ggtatgtttg tcatgcttct gatgacgatt attttttcag cagtcggaca atttggtgat
     1501 ttggtcgaat ctgcaatcaa acgtcattat gatgtgaaag actcgggaaa tatcttacca
     1561 ggccatggtg gaatcttaga ccgtttcgac agtttgctgt ttgtatttcc tatcatgcat
     1621 ttatttggca ttatttagta agtatacctg cgtttctttg tgaatttcat aaaagacgca
     1681 ggtttttatt tttcattata ttgatactat tggtgtcttt ggtatactat ggtaaaatta
     1741 tagtgtttta tcgttgtttt ataaaaaaca caaaaaacaa gtaaaggaga aaaaagctat
     1801 gaaaacgatt ctgacattta tcatcgtttt tggtatatta gtgattgttc atgagtttgg
     1861 tcatttcttc tttgcgaaac gatcaggaat cctcgtccgc gaatttgcta tcggtatggg
     1921 tccgaaaatt tatgggcatc aagcaaaaga cgggacaaca tatacgcttc gcctattacc
     1981 tattggcggt tatgttcgaa tggcaggcaa tggggatgat gaaacagaaa tggcaccagg
     2041 aatgcctctt tctcttttac taaattctga cggcatcgta gagaagataa atttaagtaa
     2101 gaaaatccaa ttgacaaatg ctatcccgat ggaattgagc cgttatgatt tagaagatga
     2161 gctgactatc actggttatg tgaatggaga cgaaacagaa gtagtaacct accctgttga
     2221 tcacgatgcg acgattattg aaaacgacgg aacagaaatc agaattgcgc caaaagacgt
     2281 acagttccaa tcggctaaat tgtggcagcg tatgctgacc aactttgctg gacccatgaa
     2341 taactttatt ttggcaatcg tgttattcat cattctggca tttatgcaag gtggtgttca
     2401 agtgacgaac actaatcgcg taggagagat catgcctaat ggagctgcgg cagaagcagg
     2461 cttaaaagaa aatgatgagg tagtcagtgt ggacggaaaa gaaatccatt catggaatga
     2521 tctgacaaca gtcattacga aaaatccagg taaaacatta gactttaaga tcgaacgaga
     2581 aggtcaggtg caatctgtcg acgtgacacc gaaatctgta gagtcaaatg gcgaaaaagt
     2641 tggccaactt ggtataaaag ccccaatgaa tacaggtttt atggataaga tcattggagg
     2701 gacgcgtcaa gctttcagcg gttcattgga aattttcaaa gccttaggat cactatttac
     2761 aggtttcagt ttagataaat taggcggacc tgtcatgatg taccaattgt cttcagaagc
     2821 agcaaatcaa ggtgtaacaa ctgttattag tttgatggcg cttctttcaa tgaatctcgg
     2881 aatcgtcaat ctgcttccga ttcctgcctt agatggcggg aaattagtct tgaatatttt
     2941 tgaagggatt cgcgggaaac cattaagtca agaaaaagaa ggaatattga cattagctgg
     3001 ctttggattt ttaatgttgt tgatggtgct agttacatgg aacgatattc aacgattctt
     3061 tttctagaaa aagttgacaa atagggtata ataacaaacg ataactattt tgaggaaata
     3121 aaggagtata tagaatgaga caatcaaaaa tgttgattcc aacattacgc gaagttccaa
     3181 atgatgcaga agtattaagc catcaaatct tgctaagagc tggatatatc cgccaagtat
     3241 cagcgggtat ttattcatat ctgccattag caaatcgtgt attggaaaaa ctgaaacgca
     3301 ttatgcggga agaatttgaa aaaatcggag cggtagaaat gctgatgcct gccatcttgc
     3361 cagcagaact ttgggaagaa tccgggcgtt acgaaacata tggaccaaac ttgtatcgtt
     3421 tcaaagaccg caatgatcga aaaatgatcc ttggaccaac tcacgaagaa acttttactg
     3481 atttgatgcg taatgaaatc aattcatata agaaattacc tttaaatctt tatcaaatcc
     3541 aacctaaata tcgtgacgaa aaacgacctc gcttcggttt gcttcgcgga agagagttta
     3601 tcatgaaaga tgcatattct ttccatgcaa cggaagaaag tttggacgag acatataaag
     3661 attacgaaaa agcctatacc gaagtattca agcgttgtgg cttagaattt cgctcgatca
     3721 tcggagacgg cggcgcaatg ggcgggaaag actcaaagga attcatggcg atctcagaaa
     3781 tcggggagga tacgatctgt tattcgactg aaagtgatta tgctgcaaac ttggagatgg
     3841 ctacaagcta ctatgtatca aaaaaatcgc atgaaacaca gcttgaattg gagaaagtag
     3901 ctacacctaa cgtaaaatca attgacgaag tagcagattt cttttctgtt gaacctcaaa
     3961 aaatcatcaa gtctgtattg ttcattgcgg atgaacaacc tgtccttgtt ttggtacgtg
     4021 gagaccatga agtcaatgac gtaaaactga aaaacttttt atctgctgac tttttagaag
     4081 aagcaacaga agaagaagca caaaaatatc taggtgctga gtttggttct gtcggaccag
     4141 tcggtgtttc agatgacgta aaagtatatg cagaccgtta tgtccaagat ttagcgaatg
     4201 ccattactgg agcgaacgaa acaggattcc actataaaaa tgtgaatcca aaacgcgatt
     4261 tcactgtgct ttcttatgaa gatcttcgtt ttgtacaaga aggagatcct tcaccagaca
     4321 acaacggtgt tcttgccttt actcgaggaa tcgaaatcgg acatatattc aaaatcggca
     4381 ctcgttacag tgaatctatg ggagcaactg tactagacga aaatggacga gaaaaatttg
     4441 tgattatggg ctgttatggt atcggtgtaa gtcgtttatt atcagcaatc gtagaacaac
     4501 attcagatga acaaggaatc aattggccaa aaggaattgc gccatttgat ctgcatatcg
     4561 tccaaatgaa tctaaaagac gaataccaaa caagcctgac gaatgaacta gaagaagcga
     4621 tgacaaaagc tggctatgaa gtactggttg atgacagaaa tgaacgcgca ggagtgaaat
     4681 tcgcggattc tgatttgatt ggctgtccaa tcagaatcac tgtcggtaaa aaagcagtgg
     4741 atggaatcgt agaagtgaaa atcaagaaaa ctggcgaaat ggttgaagta cgtaaagaag
     4801 aattggcgaa tactttacca atcctcttga accaaggcga ataataagcg ttaaaacaca
     4861 gaaaataaaa atagctgaat cagctgtttt tgacaatctg atgattcgct tttaaaattg
     4921 gaactgcgat ttgaatcgca gttccaattt tttaatgtaa aactcagaaa aaacgagtat
     4981 actaaaaggt actgaaaaag aagaggataa gatgcgatca attgcattgt ttctcatcag
     5041 aaagggcgta tatctttgtc agaaaaacga gatttatttg aaaaactgtt ggaacaaatc
     5101 caactagagg aagcagaaaa aaatcatcca ctgcttactg ctggtgaaat ggatagtgta
     5161 gtggtccatc gtaaatcgcg tttatgggaa tttacgctac atttttccaa aattttgcct
     5221 attatgctat atcggtcatt gactcagcat ctgactatcg cttttaaaga tattgcacaa
     5281 gtcaaactga atatcttagc agatgaccag acattcgatg aacagctctt gcaagattac
     5341 tgggcccaag ctttggaaaa tcagcaatgc gatacaccac tagcacagca agtattgaaa
     5401 acgcaggtac ctgtgataaa agatcgtaaa gtcatcttgc caatcgacag tacaggagct
     5461 atttcttatt tgaagcaaca atacttgcca ttagtagaag agttgttcgt cagttacgga
     5521 ttcccaaaat ttcgtatcga accagaagtg gatgagcagc aagcggaacg tgtattgaaa
     5581 ctttttgaag aacgcaaaca agaacaggca gaagccttta tgaagcaagc agctgaaagt
     5641 ctagttgtcc acgaacaaaa gaaaaaggaa agaaaagagc aatcgccagc tcttgaagga
     5701 cccatccagc taggacgaaa tattccagcc gatgaaccaa cgacaccaat gatcagtatc
     5761 gttgaagaag aacgaagagt cacacttgag gggtacgtat tcgacaaaga agtcagagaa
     5821 ctacgttcaa aacgcaagat cttgactttg aaaatcacag attatacttc atcatttatc
     5881 gtcaaaaaat tctcgaacgg cgaaaaagat gaacaagtat ttgatgcgat cagtgtcggt
     5941 agctggttaa aggtacgagg aagtattcaa gaagatacgt ttgtccgtga tttggtcatg
     6001 aatgcgcaag atatcataga agtcaaacat acacctcgca aagattatgc tcctgaaggg
     6061 gaaaaacgag tagagcttca tgttcatagc aatatgagta cgatggatgc aacaaacagc
     6121 atctctgatc ttgtggcaca agcaggaaaa tggggacacc gagctatcgc cattacagac
     6181 catggtggtg cgcaagcctt tccggaagca catagtgctg gaaaaaaagc aggtgtgaaa
     6241 atcttatacg gcgtggaagc gaatgtcgta gatgatggtg tcccaattgc ttataatgat
     6301 gcacatgaag cattgagtga agccacatac gtcgtatttg acgtggaaac gacaggattg
     6361 tctgcagtat atgacacgat catcgagcta gccgcagtca aaatgtataa aggaaatgtg
     6421 atcgaaagct ttgatgaatt tatcgatcca ggacatccat tgtcacgaac aacggtagat
     6481 ctgactggta tcacggatga aatggttcga gggtcaaagt cggaagaaga agtattgcgg
     6541 atgttccttg aattttcaaa agatacaatt ttggtagccc acaacgcggc ttttgatatg
     6601 gggttcttga atactagcta cgcaaggtat ggtattcctg aagcggccaa tccagtcatt
     6661 gatacgttgg aattagctag atatctgtat cctcaattca aacgtttcgg attaggtgtg
     6721 ttatctaaaa agtttggtgt tagcttggaa cagcatcacc gagcaatcta tgatgctgaa
     6781 gcaactggtc acttagcatg gatctttgtg aaagaggcaa tggacaatca taacatgctt
     6841 tatcatgatc agctaaatga gcatatcgga gaaggagatt cttacaaaag ggctcgacct
     6901 ttccatgtaa cgattttagc aaaaaatcag gcaggactga aagatttgtt caaactgatt
     6961 tcaatgtcga atgtcgaata cttcgaacgc gtcccacgaa taccacgctc ccaattgaaa
     7021 aagatgcgtg aaaatctatt gatcggctca gcttgtgata agggagaaat attcgaagcc
     7081 atgatgcaaa aaggggtaga agaggcaaga aaccgagcaa aattctatga ttatattgaa
     7141 gttatgccaa aagcggtcta tgcgcctttg atcgagcaag aattggtaaa aaatgaacat
     7201 gatttggaag aaattatcca aaatttggta gaaatcggaa aaagtttgga taaaatcgtt
     7261 gttgcaacag gtaatgtaca ttatctgaac gaagaagatg ccatttaccg aaaaattttg
     7321 atcaattcta tgggcggagc gaatccgttg aatcgtcata gtttgccgga tgtccatttc
     7381 cgaacaaccg atgagatgct aactgcattt cactttttag gggaagaaac agcgaaagag
     7441 atcgtagtag aaaatacgaa taaaatcgca gatatctgcg aagaagtgat tccagtcaaa
     7501 gatgaacttt atacaccgaa gattccaggt tcagaagatg aaatatcaga attgagttac
     7561 acaaaagcaa aacaaatgta cggagatccg ttacctgaga tcattcaaaa acgattagaa
     7621 aaagagttga attctattaa tggtaatggc ttctcggtta tttatctgat cgcccaaaaa
     7681 ctagtgcata aaagtaatga agatggctat ctagtaggtt cacgtggctc tgttggctct
     7741 agctttgttg cgacgatgac aggaatcact gaagttaatc cattagcccc tcattattat
     7801 tgtccagagt gccaatattc cgagtttttt gaagatggta cgtatggttc tggatttgac
     7861 atgccagaaa aacaatgtcc aaaatgtggt gcacgcttaa acaaagacgg tcatgatatt
     7921 ccatttgaga catttttagg attccatgga gacaaagtgc ccgatattga tttgaacttc
     7981 tcaggtgatt atcaagcaga agcccataac tatacgaaag tattgtttgg cgaagactat
     8041 gtttaccgtg ccggaacgat tggtacagtc gccgacaaaa cagcatatgg ctatgtaaaa
     8101 ggatacgaaa gggacaataa tctgcagttt cgaagtgcag aagtcgacag attagcaaaa
     8161 ggcgctactg gagtcaaacg gacaaccggc cagcatccag gggggatcat cgttattccc
     8221 gattatatgg atgtctatga ttttacgccg atccaatatc cagcagacga tcaaaattca
     8281 gaatggaaaa cgactcattt tgatttccac tcgattcacg ataatgtatt gaaattagat
     8341 attctagggc atgatgaccc aactgttatt cggatgcttc aagatttatc agggatcgac
     8401 ccacaaacga tccctacgga tgacccggaa gtgatgcgga tatttgccgg tcctgaagta
     8461 cttggagtaa gtcaggaaca aatttattcc aaaacaggga cattaggtat tccagaattc
     8521 ggcactcgtt ttgttcgagg gatgttagaa gaaacgcatc ctacaacttt tgcagaacta
     8581 ctgcaaattt ccggactatc tcatggtaca gacgtatggt taggaaatgc ggaagaattg
     8641 attcgacgag gagatgcgac actggcggaa gtaatcggct gtcgtgatga tattatggtt
     8701 tatttgatcc atgctggatt agatagcggg atggcattta aaatcatgga aactgttcgt
     8761 aaaggacaat ggaacaagat ccctgatgaa ttgcgagaaa cctatctatc ggcgatgaaa
     8821 gaaaataatg tgcccgactg gtatatcgat tcttgttcaa aaatcaaata tatgtttccg
     8881 aaagcccatg ctgcagctta cgtattgatg gctcttcgtg tcgcttattt caaggtttat
     8941 ttccctattt tgtattactg tgcatacttt tctgtacgtg cagacgactt tgatctagtc
     9001 tccatgtgca aaggaaaaga tgctgtgaaa caagcaatga aagaaatcac ggacaaggga
     9061 ttagatgctt ccgtaaaaga aaagaatcaa ctgacagtgc tggagttagc caatgaaatg
     9121 ctggaacgag gattcaaatt tggaatgatt gatttgtata aatcggatgc cgttaacttt
     9181 gttattgaag gtgatacgtt gatcgcacca tttagagctg tcccaagttt gggaaccaac
     9241 gtggcgaaac aaatcgttga agcaagaaaa gacggtccat ttttatccaa agaagatctt
     9301 gccacccgcg gtaaagtttc aaaaacattg atcgaatata tgaacgacaa tggcgtattg
     9361 aaagatctac cagatgaaaa tcaattatcg ttattcgata tgctataagc atctgttttc
     9421 ctgtctgctt gtaattttaa gcagagtatg gtatagttct tataggtaat gatactaact
     9481 gtgagtgagc ggatttttcg ctcactcttt ttaatggaat tacgcactta atttttaagg
     9541 aggcgaaact tttgagtagc gtcgttgaaa cagtcacaga aatggtgaca ccgattttag
     9601 atgaacaaaa ttttgaactt gtcgaagtcg aatttgtaaa agaaggaaaa agctggttct
     9661 tacgagtatt tattgacaaa gaaggcggta tcgatatcga agaatgcgcc tttgtcagtg
     9721 agaaactgag tgaaaaactt gatgcagcag atccagatcc gattcctcaa gcttatttcc
     9781 tggaggtatc ctccccggga gcagagcggc cattgaaaaa agaaagcgac tatgaaaagg
     9841 cacttggtga ttatgtccat gtctccttat accaaccagt agatggcgaa aaacaatatg
     9901 aaggatttct tcaatcattt gatgctgaac agcttacatt gaaaatacgc ataaaaacac
     9961 gagaaaaaga attgactttt gaccgaaaaa atattgcaaa agctcgctta gctatccaat
    10021 tttaatcacg gagaggaatc agaaaaaaat gagtaaagaa atgttaaatg cgttagacgc
    10081 tttagaagct gaaaaaggga tctcaaaaga aatcgtgatc gatgctttag aagcagcatt
    10141 ggtttctgca tacaaacgtc actatggcca agcacaaaac gtcgaagtgg aatttgatca
    10201 gaaaaaagga aagatccatg tctatgcggt aaaagaagta acggaagaag tgatggattc
    10261 acagttggaa gtctcattga aagatgcact tttaatcaat ccagcatatg agatcggtga
    10321 tacgatccgt tttgaagtaa caccaaaaga ttttggacgg atcgctgcgc aaacggctaa
    10381 acaagttatt ttacagcgtg tacgtgaagc agaaagaacg attatttata acgaattcag
    10441 tgcttatgaa aaagacatca tgcaaggaat cgtcgaacgc caggataaac gttatattta
    10501 tgtcaatctt ggtaaaattg aggcagttct ttctaaacaa gatcaaatgc caaatgaatt
    10561 ttatcaacca catgatcgaa tcaaagttta tgtttcccga gtagaaaata cgtctaaagg
    10621 tccgcaagta tttgttagcc gcagccatcc agacctattg cgtcgcttat ttgaacaaga
    10681 agtaccagaa gtctatgatg gaatcgttga aattgtcagc atcgcccgtg aagctggcga
    10741 tcgttcaaaa gtggcagttc gctcaaatga tccaaatatc gatgcagtcg gtacatgtgt
    10801 cgggccaaag ggacaacgag ttcaagcaat cgtcaatgaa ttaaaaggcg aaaacatgga
    10861 tatcgtcgaa tggaatgaag atccagctgt attcatcgca aatgcattga atcctgcaca
    10921 agtcatggat gtgatcttcg atgagacaaa tccaaaggcc tgcacagttg tcgttccaga
    10981 ctaccaatta tcgctagcaa tcggtaaacg cggacaaaat gcacgtttag ctgctaaatt
    11041 gactaaccat aaaatcgata tcaaatcaga atctgacatg acggaatttt atgaaaatca
    11101 agaacaacaa aaagaaacag aagaactgca tgatgaagca atcgttcagt cagatttaac
    11161 agacgatgaa tatgaaacaa ttgcattcaa caatgaaacg gtcgaagaaa aaccagaagc
    11221 ttaactttag taaggaggca aagagatgaa acaaaggaaa attcctttac gcaaatccgt
    11281 ggtttcaggt gaaatgaaac ctaaaaaaga gatggtccga atcacccgtt caaaagaagg
    11341 ggaagtatcg atcgatccaa ctggaaaaat gcctggacga ggagcgtatg tctcacttga
    11401 gccggaagaa gtacaacaag cttgggataa aaaaattctc gatcgagtac tcgaaacaac
    11461 attaacagat gagttttatc aagaacttct tgactatgtc actcaccaaa aagcacgtaa
    11521 agagttattt ggaaaatgaa tacagttaat cggcaaaaag ccatgaatct catcggtcta
    11581 gcgatgcgcg cgggaaaact aatcacgggt gaagaactga ccattgctga aatccgcaaa
    11641 caaaaagcaa aattggtgtt tgtggcaacc gatgcaagtg agaatacaag aaaaaagata
    11701 aaagataaga gttcatatta tgaagttcct tgctttgagc tattttctga agcagaaatc
    11761 acgcagatga tcggaaaacc tcgcaaggta ttcggaataa tggatgccgg atttgcgaag
    11821 aagaccaagg agctaattga aggttaggaa ggtgattgca tgggcaacaa aagaatctat
    11881 gaattagcaa aagaactcaa aaaatctagt aaagacgtcg tggaaaaagc acaacaactt
    11941 ggcatcgatg tgaaaaacca tatgggtgcg attactagca gtgatgagaa gaaattgcaa
    12001 caagcgttca aaaacccaag cacgaaacaa caagctgcac aacaagcaag cagaaaacca
    12061 gccaatcaac agccgaacga tcaacgtaaa aaagaaacaa cgaacaatca acaaaaaaat
    12121 aaaaataacc gcagttatca agatcgcggt caagggagcg gtcaagtgaa tcaaggaaaa
    12181 aaaccaacaa caaaccaaaa taatacggga aataccagca gccaaaatcg tcaaggaacg
    12241 agccaaggcg gcaatcaaaa ccgtactaac aataacaaca aaccaaacaa taacaatcgc
    12301 ggcaaattta ataataataa ccgtaatcgt tataataaaa aaggtaaaaa aggcaaacag
    12361 caacaatcaa ctaaaccagc tgtaccacca cgcaaattcc gtgaattgcc agatgtatta
    12421 gagtatactg aaggtatgaa cgtagcagat attgcgaaaa aaattcatcg tgagccagca
    12481 gaaatcatta aaaaattatt tatgcttggt gtcatggtca atcaaaacca agcactggat
    12541 aaagatacga ttgaactgtt agcgacggac tatggcatgg aaccacaaga aaaagtccaa
    12601 gttgacattg ctgatatcga taaattcttc gaggcagaag aagtcgatcc agataaatta
    12661 gtctcacgtc cgccagtagt aacgattatg ggacacgtag accacgggaa aacaacctta
    12721 ttggatacat tgcgccattc acgtgtaaca agcggcgaag ccggcggaat cacacagcat
    12781 atcggtgcct accagatcga tattgatggt aaaccaatca ctttcttgga tacaccagga
    12841 catgcggcct ttacaagtat gcgtgcacgt ggagcaagta tcacagatat cacgatctta
    12901 gttgttgctg cagatgatgg cgtcatgcca caaacagttg aagcaatcaa ccatgccaaa
    12961 gctgcaggcg ttccaatcat cgtcgctgta aataaaattg ataaaccagg tgcaaatcct
    13021 cagcatgtta tgcaagaatt aagtgagtat gaattaattc ctgaagcatg gggtggagaa
    13081 acgatttttg ttgagatctc agcgaaattc ggccaaaaca tcgaagaatt gctggaaatg
    13141 atcctgttag tcgcagaagt agaagattta aaagcagatc caacacaacg cgcaatcgga
    13201 acagtgatcg aagcacgtct tgataaaggt aaaggtcctg tgtcaacctt acttgtccaa
    13261 gaaggaacac ttcacgttgg tgacccaatc gttgtcggta atacttacgg acgtgtccgt
    13321 gtaatgacaa acgatcttgg ccgtcgcgat aaagaagcag gacctgcaac gccagtagaa
    13381 atcactggct tgaatgatgt acctcaagca ggtgaccgtt tcgtagtctt cgaagacgaa
    13441 aagacagcac gtgcagcggg tgaagaacgc gcaaaacgtg cattattgga aaaccgtgct
    13501 gccacaagcc gtgtgacttt ggacaactta tttgaaagcc tgaaagaagg cgaattgaaa
    13561 gaagttaacg taatcatcaa agcagatgta caaggttcag cagaagcact ggctgcatca
    13621 ttgaagaaga ttgatgtgga aggtgtccgt gtaaaaatcg tccattcagc tgtcggagca
    13681 atcaatgaaa gtgatgtaac cttagcagca gccagcaacg ctattatcat cggtttcaac
    13741 gttcgtccta caccgcaggc aaaagtgcaa gctgatactg aagaagtcga tattcgtctt
    13801 caccgtatca tctataaagc catcgaagaa atcgaaactg cgatgaaagg gatgctagac
    13861 cctgaatttg aagaaaaaat caccggtcaa atgatcgttc gtgaaacctt caaagtttct
    13921 aaagttggta cgattgcagg tgcctatgta acagaaggat atattcgtag agatagcggt
    13981 gtccgtgtaa tccgtgacgg aatcgtaatc tatgaaggcc aacttgcaag tctgaaacga
    14041 ttcaaagatg acgtgaaaga agtcaaaatg ggttatgaat gtggtgcgat gattgaaaaa
    14101 ttcaatgaca tcaaagtaga tgacgtaatt gaaggattta tcatggaaga agtcaaaaat
    14161 gactaaaaac tgataaaagg aggccaatca ttatggctaa ctaccgtgat cgccgagtcg
    14221 gtcaagaaat cttaaaagaa gtcaatgata ttttgcgcaa aaaggtccgt gaccctcgtg
    14281 tggagaacgt aacgatcaca gatgtgaaag ttacggggga cttacagcaa gcaacgatct
    14341 attacagcat tttatctgac ttagcatcag ataagaaaaa agcacaagat ggattgaata
    14401 aagcatcagg attgattcgt cgagagttag ggcacaatct aagtatttat aagacacctg
    14461 aattgttctt tgaacttgat gaatcagttg tctatgggaa ccatatcgat gaattgcttc
    14521 gcgacttgaa caaagactaa tcaaaaaatc ccgcaaatcc tgtgaaatca ggggttgcgg
    14581 gatttttttg ttttcattaa gacgtattac ctaagagttc catgacttta gtctggtagc
    14641 gtgtcagcta accaaaaaag agaaaaagaa aagtaaaaaa ttcgactttc ttttttctat
    14701 agtatataaa tttttgcata tagataaaga tcaagatacc gataacggaa gctagattgt
    14761 aatctatttt agtgacagtc aggtatagcc aagtaactag aagatcagtg gaacctgcac
    14821 cataaaagtt tgagtttgtc ggtccgttga cagttaaaag aaaaatcacg ttgaagttat
    14881 tgatatttcc gattacgccg cttcgatttg gtcacgatcc aaattttgga taatccctgt
    14941 agaaattagc atagtagctg ggataccaat tcacatattg acaaagataa cagagacttt
    15001 tacataaaac ggatcaatca ggaaaggaat tgctcgatcc agccccagtt ttccaacaat
    15061 gtgttaactg gcctagcgtc attcagcaaa ttatgcatga tgaggagcga aatgaactgc
    15121 ggaacagcca ttgtgataac gacaagtttt ttggatcaat gtaaaaaacg tcagcgaaag
    15181 gaaaaatttc gtggtttttt atacctgttc atttggctag atataaaatc caaacaaatg
    15241 aacagaagtg cttattaggt ttcaattggg agtatctgcg ctagaataag agtaataatg
    15301 aaaaggagtg ttatgtatgg attattctag taaagaatac tttgataaaa tgacggcttg
    15361 gtggcgtgct gctaattatc tttctgttgg gcaactttat cttaaagata accccttact
    15421 gagacgtaca ctgaaaccag aagatgtcaa aaaacatcca atcggtcatt ggggaacgat
    15481 tcctggccaa aattttattt atgtccattt gaaccgagtc atcaataaat atgatttgaa
    15541 catgttttat atcgaagggc caggacacgg cggacaagtc atggtatcga atgcttattt
    15601 agatggaagt tacacagaga tctatccaga agtaacggaa gatgagacag gaatgcaaaa
    15661 attattcaag cgattttcat ttcctggagg cattgcttct catgcagcac cagaaacacc
    15721 aggttctatc catgaaggcg gagagcttgg atactctctt tcacatgcag tcggagctgt
    15781 attagacaat cctgaagtaa tctctgctgt cgtgattggt gatggtgaag cagaaaccgg
    15841 tcctttagct ggaagctggt tctcaaatgt ctttatcaat ccagtgatag atggagctgt
    15901 tttaccgatc cttcatttaa atggtgcaaa aatagccaac ccaacgatct tggcacgtaa
    15961 atcagatgga gaattagcaa attactttaa cggacttggc tgggagcctt tctttattga
    16021 aggaaatgat ccagaaaaat taaatcctgt tatggctgaa aaaatggatc aagcaatcga
    16081 gaaaatcaaa tccatccaaa aagaagctcg cctgaagaca gcaacagatg tcgtgatgcc
    16141 aaaatggcca gtattgatcg tacgcacacc aaaaggatgg accggtccaa aagaatggga
    16201 cggggaacca attgaaggaa ccttccgagc acaccaagta ccgattcctg tagatcaaga
    16261 acatatggat catgcagatg ctttactccg ttggctgaaa tcctatgaac cagaaaaatt
    16321 atttgatgca caaggacgta ttttagaaga aatcagagaa attgcgccta ctggtgacca
    16381 aagaatggca aaaaatccta tcaccaatgg tggaatcgat ccaagaccgc ttattatgcc
    16441 agattggaaa aaatatactt tgcaatttga aaaaccaggc tccgttaaag cagaagatat
    16501 gaccgaactt ggtaaattcg ttcgcgagat cattgagaaa aatccagaaa atttccgtat
    16561 atttggacca gatgaaacaa aatcaaaccg attgaatcaa gttttcaaaa cgacaaatcg
    16621 ccaatggatg gaaaaaatcg aaccagaaaa tgatgaatgg ctttcgccat ctggtcgcgt
    16681 catcgacagt caattatcgg aacaccaaga tgaagggttc ttagaagggt acgttctgac
    16741 tggacggcat ggtttttttg caagttacga atcattcttg cgagtggttg actctatgtt
    16801 gactcagcac ttcaaatgga tacggaaatc acatgacctt tcttggcgaa aagattatcc
    16861 ttcgctgaat ctcattgcaa gctcaacagt cttccagcaa gatcacaatg gatatagcca
    16921 ccaagatcca ggaatattga cacatttggc agaaaagaag gcggagttca tccgtgaata
    16981 tctgcctgcg gatgcaaata cattgctcgc agtcatggat aaagcattcc gctcgtcaga
    17041 aaaaatcaat ttgatcatct cttccaaaca tccgcgtgct caattttact cagcagaaga
    17101 agcagccgtc ttagtgaatg aaggcttgaa aatcattgat tgggcttcga cagcaaaaga
    17161 ggaggaacct gaattggtca ttgctgctgc aggaacggaa tctaacttag aagcattagc
    17221 agctgtaact ttgctgctgg aagaattccc aaaactaaaa attcggttca tcaatgtcgt
    17281 agatttattg aaattacgtc atccaagtca agatccgcgt gggttgagcg atgaagaatt
    17341 tgatcaatat tttacaaaag acaaaccgat cttatttgct ttccacggtt atgaaacatt
    17401 ggtccgcacg attttctttg atcgtcataa ccatcattta atgattcatg gctacaaaga
    17461 aaatggagat atcacgacac catttgacat gcgtgtagta aatgaacttg atcgttatca
    17521 tttggcaaaa gacgcagctt taaaaatcaa aggaagtcaa gcagaagatt ttgcggaaaa
    17581 aatggatcag aaattacaag aacatcaaaa ctatattcgt gaaaatggta ttgatctgcc
    17641 agaagtattg gattggaaat ggaaaaatct tgaccaataa aatgataaaa tcgatcccgc
    17701 aaaacagaat tttttacatt gtattatttg tcaaattaag
//
LOCUS       DAZBSA010000055        17582 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000055 DAZBSA010000000
VERSION     DAZBSA010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.055"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(163..2160)
                     /gene="tkt"
                     /locus_tag="ACHGMT_001720"
     CDS             complement(163..2160)
                     /gene="tkt"
                     /locus_tag="ACHGMT_001720"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFN6835460.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMVVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2262..2513)
                     /locus_tag="ACHGMT_001721"
     CDS             complement(2262..2513)
                     /locus_tag="ACHGMT_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFN6835461.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2660..3283
                     /gene="lexA"
                     /locus_tag="ACHGMT_001722"
     CDS             2660..3283
                     /gene="lexA"
                     /locus_tag="ACHGMT_001722"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFN6835462.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3463..3681
                     /locus_tag="ACHGMT_001723"
     CDS             3463..3681
                     /locus_tag="ACHGMT_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835463.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3970..4482)
                     /locus_tag="ACHGMT_001724"
     CDS             complement(3970..4482)
                     /locus_tag="ACHGMT_001724"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFN6835464.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="ACHGMT_001725"
     CDS             complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="ACHGMT_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFN6835465.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6935..7378)
                     /locus_tag="ACHGMT_001726"
     CDS             complement(6935..7378)
                     /locus_tag="ACHGMT_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFN6835466.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7379..8167)
                     /locus_tag="ACHGMT_001727"
     CDS             complement(7379..8167)
                     /locus_tag="ACHGMT_001727"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFN6835467.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8164..9105)
                     /gene="rnz"
                     /locus_tag="ACHGMT_001728"
     CDS             complement(8164..9105)
                     /gene="rnz"
                     /locus_tag="ACHGMT_001728"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFN6835468.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9282..9869)
                     /locus_tag="ACHGMT_001729"
     CDS             complement(9282..9869)
                     /locus_tag="ACHGMT_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFN6835469.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10090..11403)
                     /gene="obgE"
                     /locus_tag="ACHGMT_001730"
     CDS             complement(10090..11403)
                     /gene="obgE"
                     /locus_tag="ACHGMT_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFN6835470.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11691..12701
                     /gene="gap"
                     /locus_tag="ACHGMT_001731"
     CDS             11691..12701
                     /gene="gap"
                     /locus_tag="ACHGMT_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFN6835471.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12786..13721)
                     /locus_tag="ACHGMT_001732"
     CDS             complement(12786..13721)
                     /locus_tag="ACHGMT_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN6835472.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            13907..14866
                     /locus_tag="ACHGMT_001733"
     CDS             13907..14866
                     /locus_tag="ACHGMT_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFN6835473.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(14952..16046)
                     /locus_tag="ACHGMT_001734"
     CDS             complement(14952..16046)
                     /locus_tag="ACHGMT_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFN6835474.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            16235..16996
                     /locus_tag="ACHGMT_001735"
     CDS             16235..16996
                     /locus_tag="ACHGMT_001735"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFN6835475.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(17111..17551)
                     /gene="fabZ"
                     /locus_tag="ACHGMT_001736"
     CDS             complement(17111..17551)
                     /gene="fabZ"
                     /locus_tag="ACHGMT_001736"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFN6835476.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT         5052 a         3830 c         3084 g         5616 t
ORIGIN      
        1 tctcaagaaa cacaaactaa tccgaagaat tctttgtttt gtcatttaaa tgtaacatag
       61 ccgaaaacga aaagtaatat tgatttattc ttctaacaca taaagaacag ctgtctccaa
      121 cttgcagaca gctgttctta tcagtcaaac aattgcttgg atctatgata gttgattgta
      181 cgtattcacg acattttcta ctgtaaatcc aaattcttct aaaattttag tgccaggggc
      241 agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct gttcctacgt agcgttccca
      301 tccaaatgat gcagcagctt cgatagccac acgtttcttc actgatttag gaagtacact
      361 ttctttatat tcagaagatt gtttttcaaa gaggtcaaag cttggcatag aaacaacaga
      421 aacatctttg cctttttcag ccaattcttt ttgagctttc acagccaaat caacttcaga
      481 acctgttgca atcaaaatac cttctggtgt ttctccttgt gatggagaaa gaacatacgc
      541 gccttttttc accatttcat ctgctacttc ttttgtagaa ggcaatactg gaaggttttg
      601 acgactcaat acaaggatag taggcgcatc tgttgtctcc atcgctactt tccaagcagc
      661 acgtgtttca tttccgtctg ccggacgaat cacttgaaca ccaggcatgc agcgaacaga
      721 tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct ccgacagcaa ccgagtcatg
      781 tgttaagacg taaacaacag gagtatgttg gattgctgct aaacgaactg ctggtcttag
      841 ataatcgaca aagacaaaga atgttccacc gtagatacga gttccgccat gaagttggat
      901 accattcata gccgaagcca ttgcaaattc gcggacaccg aaccagatgt tgcgtccttc
      961 ataatgttca ggagtgaaat ctttatctgc agccaccatt gtattattgg aacctgacaa
     1021 gtccgctgaa ccaccccaga agcttggtat agctttcgat aattcttgga tgacttcttt
     1081 acttgaaaca cggcttgctt gactgcttcc aacttcataa gttggcaatt ctgcatccca
     1141 gttttctggt agtttgccat caaaagcatc ttcaaattgt tgtgctaatt caggataagc
     1201 ttttttataa ttggcaaaca tttcattcca tgcatcttca gctttttgtc cttcttctat
     1261 catcgtttga tggaagcggg cagctacttc ttctggaaca gtgaaatctg gatactccca
     1321 accataaact tctttagcca tcttgatgcc atcttcacca agaggagcac catgaacagc
     1381 agatgttcct tctttaggag caccatagcc gatgactgtt tttacttcga tcaatgttgg
     1441 tttgtctgtt tcagcttttg cttcttcaat cgctttagag attgcttcta ggtcatttcc
     1501 gtctttcact aagatatgtt gccagccata tgcttcatag cgagcaccaa cgttttctgt
     1561 gaaagctttt gaagttggac cgtctagtga aatatcgttt gaatcgtata aaacgatcaa
     1621 tttccctagt ttcatatgac ctgccatcga acttgcttct tgagataccc cttccatcaa
     1681 gtctccgtca ccgcatattg catacgtata gtgatccatc acattaaagt tttctttgtt
     1741 atacgttgct gcaaggtgcg cttctgccat tgccataccg acaaccattg caatcccttg
     1801 tcctaatggc cctgtagtgg cttctacacc atctgtatga tgtacttcag gatgtcctgg
     1861 tgttttagaa tcccattgac ggaattgttt caaatcatcg attgtcactt gatagccagc
     1921 taaatgaagc aagctataca acattgctga cccatgtcct gccgaaagaa cgaaacggtc
     1981 gcggtcagcc cagttttttg atgtcgttgg atttactttc aggtgttttg tccataacgc
     2041 atatgccata ggtgctgcgc ccatcggcaa tccaggatgt cctgagtttg ctttttggac
     2101 tgcttcaata cttaatgtac gaattgtgtt aacgccaagt tggtcaattt tgtcaaacaa
     2161 aataacccct cctattaatt taggtttttt ttacctttat ttcttacact ttcattctat
     2221 aagatgtcac acgaaaaagc aatctttttc agtatttttt actaaaagtt gtcatttcgg
     2281 ttgtgtaagc cttttttctt ttggatttct tttaattttt ccggggtcac gtcattgccc
     2341 tcaggatcga cgactttcat tccttcgata tgatgacgca taccggaacg aaaagcttcc
     2401 aaatactctt tgcgcaagtt gatttgttct tgttcttcgg cttttgagag accattttcc
     2461 ttctttttac gtgcaagttc attgatccgc tctatttttt caggtgacag catgctcagc
     2521 cctcctttta ctctccttca tattatataa aaactgaaaa aaaaacaact agataaacta
     2581 aataaaatac gaacattcgt ttgatttagc gctttttata tggtactatt tatttatcaa
     2641 gtgaaagaag gtgtaccttg tggcccgaca aactgaatca agacaaatcg aagtcttacg
     2701 atatatccat gaacaagtaa cccaaaaagg atacccgccg actgtccgtg aaatcgggga
     2761 agctgttcag ctttcttcta cttctactgt tcacggtcat cttgcacggc ttgaaaaaaa
     2821 agggtttatc caaagagatc cgacgaaacc tcgtgcaatc gagttgacaa aacaaggttt
     2881 agaaaagatt gggattcgtc caacaactat tccagtactt ggcgtcgtaa cagctggaga
     2941 acccatttta gcagtagagg atgcttcaga cttctttcca atccctccta atctttcttc
     3001 cgaagaaggc agtctcttta tgctgacaat tcgtggagat agtatgatca atgctgggat
     3061 attggatggt gacaacgtga tcgttcggaa gcaagaaact gctcaaaatg gcgatattgt
     3121 cattgcaatg actgccgagg acgaagcgac ttgcaaacga ttctataaag aaaaaaactt
     3181 tttccgtctg cagcctgaaa acgacttgct cgaaccaatc atcttagatc aagtatctat
     3241 attaggacga gtagtaggac tttatcgtag tcatatctat taattgctac taattaaaag
     3301 aaaacttttt aaaaagagtt tgagacgttg gtctcaaact cttttttcgc ccgggttctt
     3361 cttctttcaa atttttatca cttccctctt tacaaaccga acaaatattc gtatactaaa
     3421 tatacgaaca attgttcgca taaattaaag aggtgagaat tcatgaaatc aatgagacgt
     3481 cttggcttag tattaactgt tttgttttta ggtattatta ttggtaattt cggcatacgt
     3541 ccagctttac ttgtgtttgt tccgttattt actttgtggt tcatgttgtg ggatgaaaag
     3601 aagtaccgtc aatctgagcg gcgcagaatt catgaagatc atttctatca tgagccctac
     3661 tacagatcca ggcaatcata ataaggattg actgctaatt attcaaaaga caagaagtta
     3721 aacttttacg ggggtgtgac aaaaatcggt cacatccttt ttttcgaata aacggtgttc
     3781 taaagccagt ttttcggtat taagtcaaat tgcggaaaaa gtgatttttt aaacttctac
     3841 tggtctttgc ttgtttccat ttataaaaaa atagcgaccc tcaaaagtca gatagagaat
     3901 ctgacttttg agggtcgctt taaaatcgct tctttaagaa taacatcaag aatagcttct
     3961 tttttctgct taatattcca ttaatgtaat gatgtcatat cctttgattt tgtcgcgtcc
     4021 atggagatcc atcaattcga tcaaaaatgc gcagcctacg actacaccgc ctaattgttc
     4081 gatcagttcg atcgtcgctt tgattgttcc tccagtagct agcaaatcgt cacaaatcag
     4141 cacacgttga cctggtttaa tggcatcctt atgcaatgtc aatgtatctg ttccatattc
     4201 taatccataa gtcacttcga tggtttctcg aggtaacttc ccttttttgc ggactggggc
     4261 aaaaccgact cctaactcat aagcaacagg acatcctacg atgaatccac gggcttctgg
     4321 cccaactacc atatcaattc gtttttcttt tgcataattt acgatttgtt tagtagcttc
     4381 ccgataagcg tcaccatctg ccatcagtgg ggaaatatct cgaaacacaa ttccttttga
     4441 cggatagtcg ggaatactgg caatataatc ttttaaatcc actctttgtt tcctcctagg
     4501 ttgttaacca tttctttatg gtaggaatat cgctcaaaag caaaaattcc tcaactttga
     4561 ttttattgag ccgtcgctgg tataatctgc tctcagtcaa aggatgactt tctgggctag
     4621 cgactttttg taaaacaccg ttctctattg taacaaattt caattcaaaa aacacctgta
     4681 tcataaaaat taataatttt tcagggatac gcaaatattg agctattgct tttattttat
     4741 gacgtatatc aattcgttct tgagaatgga tcaatttaaa caattttgcg tactgttctc
     4801 ttgtgcctac cccattcaaa taggcctcat caggtgaaat ccccatcaga taaacacgat
     4861 tgaatgaata agaccgaaaa acttctttca gatccgctaa atcatctgga caatccataa
     4921 caactagaga gtttggtttt cttttttcaa ttattgcctt tacagaatct agtccttcga
     4981 aaacaatcgc agcttcctgg ataatctcag gcacgaattt caacgtcgaa gaatcaaatg
     5041 ctaaataaag gctaccttcc gacgttcggc tctgttcacg gaaacgtttt gcccgccaat
     5101 caaatacttg cagaccaccc acagaaaaat cagataccat cagctgcggt ttcttacgcc
     5161 cgttccattc attgatcgaa agctttccta ccacatctac ctgattattc agcagttctt
     5221 cttcttgagt accaaaacca aaagcgaccg catcaagctg actcgatgca tcggaaagga
     5281 caaatttcag atgctgttgg tttgctccga ttcttttgat attttctgct tggacttgtc
     5341 gaaataaaaa ctgaggcaaa ggattatccg taccaaatgg cgccaatagt ttcagctcat
     5401 caattcgctc tactgttact tcatttggca gcaatacttc atcaattctc aactcaggcc
     5461 ctctcatcaa gtcaatttga tgatcgacta tatattggtt catcttctcc tgtagtatag
     5521 tgacattttc acttggcatc gttaaaccga ctgctgcatg atgtcctccg aagtaagtaa
     5581 ataaatctcg catctggtca agcatttcaa aaagattcaa ggcttcaata ctacgtccag
     5641 atcctttagc tgatccatct tcttttaaag taaggacaag cgttggttta cccgtttcat
     5701 tcattatttt cccagcaaca atgcccaaaa cgccttcatg ccatcctcgg tttaccaata
     5761 gatggatatg attctcctta ttgaccatcg ctaatgcttc ttgtgttatc tgctctacga
     5821 ttgcttttct ttcttcattg atctcattca atttttttgc ttgaacggat gcttcctctt
     5881 catcaaacgt cgcaagtaac gagacggcag gacgtggatc gcccatccga ccgattgcat
     5941 ttagtctggg agcaatcgaa aaaccgatcg ttgtttcgtc tgcgtccctc atttttacgc
     6001 cgctttcttc aaatagcgcc tgaagtccta tccgttctga atgatgaatg gcatcgattc
     6061 ctaatgaaac aaggattcga ttctcatcag tcaatgaaac taaatcggcg atcgttccta
     6121 tcgctactaa atccaaaaat tctgcaggcg gttcttccaa taatgcagaa gctactttga
     6181 atgctactcc gactcctgcc aaatctggaa atggatagtg ccccgctgga tgacgagggt
     6241 gaacaatcgc ataagcgtct ggtaaattct caggtagttc gtgatgatct gtaatgatca
     6301 cgtctacacc agcttgctga gcgtatgcta ccgcatcatt tcctgctacc ccgttatcaa
     6361 cagtgatgat caactggatt ccttcttcga tcttttcttg gtataccgat tggtttggtc
     6421 catacccatg ctcaaatcga tttggcagga atacttctac ttctgcacca agtaattcca
     6481 gtgtttcttt cattaccgtt gcactggtga tcccatctgc gtcataatca ccgtaaacga
     6541 ggattttttc tccactgatt acagcttctt ggattcggct gattgcttta tccatatcgt
     6601 gtatcagata cggatcatgt aattcttgtt cctttggatg taaaaatcgg ttgatatcct
     6661 tttcctcatg gtaacctctt tgccacagta acttaccgac aaatggagat aatttcagtt
     6721 cacggatcat ttggagaaag gattcagaag gctctgtctc gcttaacttc caatcaaaat
     6781 ttgcttgtct cactttctca ctcctaactt agttattata gcaaaggaaa tcagcaaaaa
     6841 aaagcagaaa gaaagaataa aaacagatca gatccgctag acagatgttc agcaggacct
     6901 gatcttttgt acgaccattt atgaaatatc cctattagtc aaaataattg aagctttgtt
     6961 tgggcgattg agttttttgt tcttccggcg ctttcaattg actgatttgt gcggttttcg
     7021 cttgtaactc cgcctgataa gcagcttcca actccgcacg ttcgtttcta aactcgcgtt
     7081 gctgttcttc ttttgcttct tcaattttct tttgaagttc tgtttgatat tctgacattt
     7141 cctgagtcag ctgtttgatt tgcttttttt gctgccatag tgttgccgaa gcagtcaaaa
     7201 atacaatcag tgcaccaatc agcgcagaac cgatgatcac taagatcaat ggcgcagaaa
     7261 aggaagtgaa gccaaaactt acaggaacat ttttattgtt caatacagca aaaagaacaa
     7321 taatcaaaac taaaacaaat ccaagaatca cattgctttg ctttttcaat tccttcacct
     7381 actttttgtt aaaaattccg ctggctaaat aatcaccaat cgttggaaac agcgtataaa
     7441 attttgatgc aacttctaaa ataaacggtt gattgatttc ccgttttgga tgtctcatat
     7501 tgcggacgat catttttgcc aacttggcag gttctaaaac gatttgtccg actttttcca
     7561 aatagcttcc gcttggatca gccttatcga agaaatttgt ctcgattgga cctggattca
     7621 ctgtcgtaac agctacgcca agtggtttca attctaatcg caaagcgttg gagaagccaa
     7681 ggactgcaaa tttcgtggca gaataaaccg ttgattttgc agttgccatt tttccagcca
     7741 tagaggcgat attaatgata tgaccttgtc ttctttctgc catatcgatc gcaaattttt
     7801 gtgtcaaaac catcattcct aagatgttga cttcaaacat atcgtatgct tttccaaggt
     7861 cgaatgtaag gaagtcttcg aaaatcccaa aacccgcatt gtttaccaga atatcgacgc
     7921 gcccgacttc ttccatcacc ttttcatata attcgtccac gctttcagga ttgctgacgt
     7981 ctaccttgaa agcataagcc tctttgccgc tcaagcgtct gcactcgtct ctgacacctt
     8041 ctacaaatgc cattcttcgt gcgcaactta taacgattgc tccttgcttt gctgcttcat
     8101 aacagatttg tgcgcccaga ccgcctgaac tacctgttac taaaacaact ttatcttcta
     8161 aattcatgcc tcttcctcct cgcgaaatgg aatctcaatg ctatcaaaat ctttcataat
     8221 cgtcgtgttt tcaaacactt cttgtgcttc tttttcaagc tctaaagctg cttttcctaa
     8281 atatctcgca ctgatatgag taagtagcag ccgttttgcc tgcgcttctt ttgcaacttc
     8341 agcagcttga tgagtcgtgg aatggaaata gttgtgcgcc attcttgctt catctttatt
     8401 aaatgtgctt tcatgtacca gaatatcgct attttccgca agcacaacgg aattatgtgt
     8461 ttttctcgta tcgcctaaaa tagtcacgat tcgtcctttt ttatctggac ctacgaaatc
     8521 ttttccattg atcgtccgcc catcttctag ttggatcgtt tcaccttgct tcagtttgcc
     8581 gtacagtggt ccagctggaa tacctaattc ttttagacga tccacctgca attctccttt
     8641 atgatcatgt tcaaccacac gataaccaaa actgtcgatc ccatgattta gtttttttgt
     8701 ataaacagaa aattgctgat cagtaaaaat cggggctgtt tcatttagct caatgaattt
     8761 cagcggataa cttaaacgcg tttgcgaaat acctaaagag acttttatgt attcctctat
     8821 cccttttggc ccgtaaatct ctaatggcgt atccccacct tgaaatgacc ggctgctgat
     8881 gagcccggga agtccaaaaa tatgatcgcc gtgtaaatgt gtaatgaaaa ttttaccgat
     8941 ttttctcgga cgaatagtcg ttcgcaaaat ctgcatttgc gtgccttcgc cacagtcgaa
     9001 caaccaaact tcattgcgct cgtctaaaag tttcaatgct atacttgtca catttctatg
     9061 ttttgcagga acacctgcac ctgttcctaa aaattgtaat tccataatct acctgacttt
     9121 ctttaagttc ctctttaatt ttagggtaaa aagaagggat aatcaaccgt aagtcgagaa
     9181 tgggttcaaa ttaaaaataa cgttctaaag cgatagcgtt ttctttgaaa attactataa
     9241 aaacagcagc gaacccgttt gagatcctct gctgtttttt attaatttga agatccagaa
     9301 ctttcattgt cgttggaagt ttgcggatgt ttgtccttcc aagtcttttg tgcagcttct
     9361 ttcgcatgat tttcagcttc tttcgcatca tgcacatctt tttcgacatc ttcataatcc
     9421 aagcgagtaa tttctccgcc taattccatc attaccaatt gattcagtgg gcgattgtcc
     9481 tcttcatcag caatcaagat cagtcctgta gctccttcac cgattttatt tcctacaaag
     9541 tcgaacacgg tctgggcatc ttttatttct ttggcatctt gtgtcgcacc gatcattcct
     9601 ccggcaaacc atcctaataa aatccctagc ggaccaccta atacaccaac caacattccg
     9661 attaatccac ccgtagaaga tttgttatta ccggtaaagt cgataaaatc ttctattttg
     9721 aattgatgtg tcccattagg atcatggctg acaactgcca tctgttctcc tttaatcgaa
     9781 cgaacagcat gtaacttttt gatttctgaa aatgcctgat atgcctggct ttctacttga
     9841 aaatttaaaa tgattactcg tttgctcatt aaaaacgccc ctttcatacc ttcttgttat
     9901 ttattttacc tttaattgaa aaataagaaa agaaaagcac tctttttttc caataaaata
     9961 gggctgtgac aaaagtcgta tcacagctcc tttttccgaa taaacggcgt tcttgaagct
    10021 gatcacttct gtcacaacct cccttaaatc tgaatctata ttttttggta tttgttttta
    10081 tttgcttgcc tattcgacaa attcaaattc aaactcaccg atacgtacaa ggtcgccatc
    10141 tttcgcaccg cgtgcccgaa gagcttcatc cacacccata ccacgtaatt gacgagcaaa
    10201 acgcatcact gtttcatcat gatcgaaatt cgtcatttgg aataattttt cgatcttctc
    10261 accagataag atccacgttg catctgaatc acgatcgatt tggaactcag gaccttctgg
    10321 attgaatcca tagtgtacag tttcttcttc cagttcttct tcgtacaatg ggaattctgg
    10381 cgttacttct aacaaatctg ctgtagcatt caataacgcg tccaatcctt gtcgagtcac
    10441 tccagaaatt ggaaatactg ggatgtcatc agcaaattca tcttcttttt ccttgttcag
    10501 ctgttctttg aatttcacta gattttccgg tgcatccggc atatccattt tgtttgccac
    10561 gatgatttgc ggacgctcta gcaagcgtaa gttataagta gataattctt tgttgatggc
    10621 aagatagtct tcataaggat ctcgtccttc catcccactc atatcgatca catgtaaaat
    10681 tactcgtgtt ctttcgatat gacgaaggaa ttgtgtaccc agcccaacac cttgcgaagc
    10741 tccttcaatc aagcctggca agtctgctgc ggcaaaactt ctgccatcac ttgtagtaac
    10801 cattcctaga ttaggtacga gcgtcgtgaa atgataagcg ccgatttttg gacgagcaga
    10861 tgaaataacg gatagtaaag tcgatttccc gacagatgga aaacctacaa gtccaacgtc
    10921 tgccaatact ttcaattcta gttcgatttt tcgctcttgt cctggttcac cgttttcagc
    10981 aatttccggt gcaggattcc gaggagaggc aaaacggata ttccctcgtc caccgcgtcc
    11041 accttttgcc acaactagtg tttgaccatt ttcaattaaa tctcctaaaa gagcaccagt
    11101 ctccgcatct cggacagtgg ttccttgtgg tactttaaca taggtatctt ctgaaccacg
    11161 accgtgcatc cctttactca tcccattttc acctggctgc gctttgaaat ggcgattgaa
    11221 ccgaaaatcc atcaatgtac gtaacccttc atcgacgatc aagatgacat cgccgccacg
    11281 accaccgtct ccgccagccg gtccgccgtc aggaacgtat ttttcgcgac gaaatgcaac
    11341 cattccgtct ccgcccttgc cagctttcac atcgatcgtt acttgatcta aaaacatgga
    11401 cataattaag tcctcctatt attcatttat ttatttgact gcctgaattt ccacaataaa
    11461 caaaagtctt acttatttta tcggtgaaat ctgattttgt ctatcatttc agcaaaaaga
    11521 ttgtttacct tcttcttttt ttatcatttg gtaaggtttc caaccttttt tctgctacca
    11581 agtataattt tttgacaaaa tattctttgc gtagcagaat aacagtgtat taattgtatt
    11641 aattgaggat gccgtttcac agcaccccta ataaaggagg aacttttgat atgacagtaa
    11701 aagtaggtat taacggattt gggcgtattg gccgtctggc tttccgccgc atcaaagaag
    11761 tctcagatga tattgaagta gtagcaatca acgacttaac aagtccaact atgttagcac
    11821 aattattgca atttgactct actcatggta cttatcctgg cacagttact gcaacagaag
    11881 actcaatcgt agtggatggc gaagcaactc gtgtttatgc agaacccgac gcaagcaaaa
    11941 tcccttgggc aaaagaaaac ggtgtagata tcgtactaga atgtactggt ttttatacat
    12001 ctgaagaaaa agcaaaagcg catttagatg ctggtgtcaa acgagtagtt atttctgccc
    12061 cagctggtgc tatgaaaacc atcgtctata acgtcaatga tgatacatta gatgaaaacg
    12121 acaaaatcat ctcagcaggt tcatgtacaa caaactgttt agcacctatg gcttatttct
    12181 taaataatga atttgggatc gaagttggta caatgacaac agttcatgcc tacacttcca
    12241 cacaaatgct cttagatgga cctgtaaaag gcggtaattt gcgtgctgct cgttcagctg
    12301 ctgacaatac tatcccacat tctactggtg ctgcgaaagc aatcggctta gttatccctg
    12361 agctacaagg aaaattacaa ggacacgcac aacgtgttcc agtcgttgat ggttcattaa
    12421 ctgaattggt ttcagtattg aaaacaaaag taactgctga tcaagtaaac gaagcaatca
    12481 aaaaacacac agttgacaat ccttcattcg ggtatgatga ccgtcaaatc gtttctagcg
    12541 atgtcattgg aacaactcaa ggttcgatct ttgacccaac gcaaacagaa gtaacaactg
    12601 ctggtgattt ccaacttgtt aaaactgttg catggtatga caatgagtat ggatttactt
    12661 gccaaatgat tcgcttacta gaaaaattcg ctaacttgta atttgataga tcaatcgata
    12721 aaagaagcct ttgactggat ccagtcaaag gcttctttct tttcatcccc tacttttaaa
    12781 ctcgttcaat gaacatattg gcttaaaaat tgttggacta cttgctgata tttttcagga
    12841 tctgtcttgt atgcttccgc atgctcggct ccttttacaa catacttttc ttttggtccc
    12901 ttcgttgcac gatagacctt atctaacatt tcaaatggta caaatgtatc ttcgtctcca
    12961 tgtataaaga gcattgggcg ctggtttttc ttcaattgtt ccactgcatt tgcttcaccg
    13021 aaaaagtagc cagcacggac ttttgtcact agactcgtta cttgcataag cggaaatgct
    13081 ggaagactaa acatatcgtc tagctgataa gcaagctcat catgaacgga agaataaccg
    13141 caatcttcaa tgattgcttt cacattatct ggtagttttt ctccgctagt catcataact
    13201 gttgctgctc ccatgctgac accgtataga acgatttctt gcgattttcc attgttttct
    13261 agcactttgt ttatccactg tacatagtct ttacgctctg gccatccaaa tccaatgtaa
    13321 tccccttcgc tttttccatg tcctcttgca tcaggaacaa ggacattgta acccatatca
    13381 tgaaacatct ttgcgtaatt ggtcatcgtt tcagcgtttc ccatatagcc atgagccaca
    13441 agagctgttt tttcactttt cgtttcagca ggaagataaa tcgctgataa tttcagaccg
    13501 tcttttgaag tgatggtcca atcgcttcgg ttttcttttg aactaaacca tttatgtgca
    13561 tccgtttctt tggatgtctg tgttgtacca ggtgtatctt cgtctaaaaa gtctttctca
    13621 gaaggcacaa cagcataatt ataaaaataa ttccctgcaa acactaacgc aacaatcaga
    13681 gctaaagcga cgcctaaaag gattttcatc caaagtttca taaatcatcc atctcatttc
    13741 tcttttttca tataaccttg aattagtata atcaaagaat ctctactatg caatgttcat
    13801 ttttataacc cgcgaattgt aaaattaagt tagaaaaata aaaaggcatt tatggtacac
    13861 tcaaattgta tttccgacga aagaaaacaa aggagtgtaa ccataaatga cctacaaaca
    13921 tcttaccata gacgaactga caatgataga atcatattat cttcaacata ataagccggt
    13981 tgaaatcgct aaccgaatgg gacgtgctat acaaactatt tataatgtag tcaataagtt
    14041 caagcaaggc aagactgctc ttgattattg gcaccagtat aaagaaaata agaaaaaatg
    14101 tggtagaaaa gtcattcaat tacctgctca tgaagtagat tacattaaag agaaagtcac
    14161 tcttggttgg acgcctgacg tcattatcgg gcgaaaagaa aggtctgttt catgcggtat
    14221 gagaacactt tatcgtttat tttctaaagg aatatttgat attgacacac taccgatgaa
    14281 aggtaaaaga aaacccaatg gccatcagga aaaacgggga aaacaacagt atcagcgctc
    14341 aatccatgat cgacctgata attatcctga tttcaattct gagtttggtc accttgaagg
    14401 tgatacgatc gttggcattc atcataaaag tgccgtcatt actttagttg aaagattatc
    14461 taaagtcatt atcacgatta aacccaacgg ccgtaaggca ttagatattg aaactgccct
    14521 taatcaatgg ttttctcgct tccctaaaaa cttctttaaa tctattacgt ttgactgtgg
    14581 aaaagaattt tctaactgga aagccattag taaccaacat gatattgata tatattttgc
    14641 ggaccctgga acaccttctc aacgcccatt aaacgagaat tctaacggga ttctgcgtcg
    14701 taatggactg ccgaaatcaa tggattttag aaaagtgaat cagacattta tttccagtgt
    14761 cagcaatcaa cgtaatcata ttccaagaaa atcattgaat tacagaacac caatcgaaat
    14821 atttttgagc tatgtacaag aagcatttta ttctagctta atttgacaaa tcataaccta
    14881 taaaaaaaca aaataaaaaa gcagaaataa ctggctattt ctagcggatt atttctgcaa
    14941 taactaatta tctatttttt attttcaatt aatttaaagc ttgaccatgg tttcaaaaag
    15001 tcaaggagga ataattcgtc atcggagatt cttcctacaa cattgacacg tccgtcgttt
    15061 ttcatatcct tcaaggcgat ttgtgtttct cctttgtatt gaccgtaccc ttcattgtca
    15121 atcagtacat ccccatgatg catatctcgc gtattatgag gcgggaaatc tttatctttg
    15181 tagtatacac gagtcatcgt tgaacgaagg atatattctg agcgatcccc tcgataacta
    15241 tgaaggttat caaacaagca gattcgttcg ttttctgtaa taccttcttc cgtatctact
    15301 ttcaatgttg gataatccgc gttaaatgct tctgccattt ctttcaattc ttcttcactt
    15361 gcgtacgcat ttccaataga aatatcatca atcaatcctg taaggattaa atgcttcact
    15421 tgtgtcgcaa tttcaagatc tcggtggtct tccagcgtgc acaagccatc ttgcgtaggc
    15481 caagggccaa actcagcaga ttgcgaattg acgaatgcca tcgtattcaa attatatttt
    15541 ctgaattttt ctgaacaaaa aacaaagtgt tcgtatccta aaccagagta tcgatgagga
    15601 taaaaattat gagaacctaa taaattatcc gtgtttggcg aataactcat gatgttgtca
    15661 acgtaattcg tgcccgaact catattgatc tcgattttga taccaaaagg attgcgcgtc
    15721 atttttgctt cttctgctcc tgtgaatccg atatctaagc ggaccccgta ggcacccatc
    15781 ttatggaaaa aagataagtc atcgtaagaa atctccaact gttcaaataa cgctggattg
    15841 atatcaacca ttacttccat gcctaggcta ttcgcgtaat caactacttc tttaaattct
    15901 gaaagaactt tttctttatc atcattgatc tgcagcaaac tggtaaaaac ccgtttatac
    15961 ccatacttat gagctaaatc caagtacgct ttgtcctttt caaatgttga ccgttctgga
    16021 taaatcgaga ttcctaattt tcccatatta ccatttcctc ctgcatcttt tcgtttttgt
    16081 atccacttac attatagcaa acacaaagta aatggtctac accttttctt tttcttttaa
    16141 aataaatgat aaattccaat aatttaagta ggtattttcc ctctattttg ttatcattaa
    16201 agataacaaa atagtaaatt ggaaaggaac agttatgaag aaaaataaag ttattttaag
    16261 tattagttta gcagcagcat tgctgtttgc tgctggttgt tctgcaaaca acacgacttc
    16321 ttctagttcg tctacagaaa gttcaaaaag tacatctact gcggaaagca cgactgccag
    16381 tagcgtcgat ttgaacgcat tggaattacc acaattgacg aaagatgtcg ccgaaaatga
    16441 agatctggtc gaaatggtga cgaataaagg cacaatcaaa atcaaattat ttcccgaaca
    16501 cgcaccaaaa gcggttgaaa actttatgac acatgcaaaa gacggttact acaacggact
    16561 gactttccat cgcgtcatca aagatttcat gatccaaggt ggcgatccta aaggcgatgg
    16621 cactggaggg gaaagcattt ggggagaagg atttgaaaca gagatcaaca accaccttta
    16681 taatataaga ggtgcgttat ctatggctcg ctcacaagat cctaacagta acggcagcca
    16741 gttctttatc gttcaaaata cagatgatat gcatgatggt cttttaaaag acgactatcc
    16801 tcaagcaatc atcgacgctt ataaaaacgg cggttatcct tcactagatg gccattatac
    16861 tgtcttcggc caagtgattg aaggaatgga tgtcgtggat gctattgcaa atatcgaaac
    16921 agatagttca gacaaaccaa aagaagaagt aaaaatcgat aaaatcaata tactgcaaga
    16981 agcaaaacaa aaataaaaaa ataacgtctg aatcctgcat agatgacaac gtatgctagg
    17041 attcaggcgt cttcttttta cataaaaagc aagtaagctt ccttttgttt tgctatatca
    17101 ggttttatag tcaagcattt tggttgacga tgaacgtgaa ctcgcactca cagacttttt
    17161 tatcatctac atatgctgca gataacgctg taccgactga tcccttcatt ttggtgattt
    17221 caaaatgaca tttcagcaca tctcctggaa caactttttg acggaatttg gccttattga
    17281 tcccaccgat ataagctctt tttcctagaa actcttctga ttttaggata agtatcgaac
    17341 ctgcttgtgc cagtgtttct agaatcaacg cacccggcat cactggattg tcaggaaagt
    17401 gaccttggaa aaaatcttca ttgattgtta cattttttgt cgcaacgatc ttttttcctg
    17461 gttctaaagt atctacataa tcaatataac aaataggata acgattagga atcaattcca
    17521 ttacttcagt agcagataat acgtaagaca taattaaact ccttctttca ggcaattgcc
    17581 at
//
LOCUS       DAZBSA010000056        17464 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000056 DAZBSA010000000
VERSION     DAZBSA010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.056"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1617)
                     /locus_tag="ACHGMT_001737"
     CDS             complement(<1..1617)
                     /locus_tag="ACHGMT_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFN6835477.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKI"
     gene            complement(1604..3292)
                     /locus_tag="ACHGMT_001738"
     CDS             complement(1604..3292)
                     /locus_tag="ACHGMT_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835478.1"
                     /translation="MKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRI
                     KGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNI
                     CTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYY
                     AYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFV
                     VVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDI
                     TTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLL
                     QFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVI
                     QSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKA
                     ADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIE
                     ITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            3438..5075
                     /locus_tag="ACHGMT_001739"
     CDS             3438..5075
                     /locus_tag="ACHGMT_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835479.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(5220..5960)
                     /gene="truA"
                     /locus_tag="ACHGMT_001740"
     CDS             complement(5220..5960)
                     /gene="truA"
                     /locus_tag="ACHGMT_001740"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFN6835480.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(6151..6786)
                     /locus_tag="ACHGMT_001741"
     CDS             complement(6151..6786)
                     /locus_tag="ACHGMT_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFN6835481.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(6933..8153)
                     /gene="sstT"
                     /locus_tag="ACHGMT_001742"
     CDS             complement(6933..8153)
                     /gene="sstT"
                     /locus_tag="ACHGMT_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFN6835482.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            8372..9370
                     /locus_tag="ACHGMT_001743"
     CDS             8372..9370
                     /locus_tag="ACHGMT_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835483.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            9566..11401
                     /locus_tag="ACHGMT_001744"
     CDS             9566..11401
                     /locus_tag="ACHGMT_001744"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFN6835484.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(11461..12177)
                     /locus_tag="ACHGMT_001745"
     CDS             complement(11461..12177)
                     /locus_tag="ACHGMT_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFN6835485.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(12404..12946)
                     /locus_tag="ACHGMT_001746"
     CDS             complement(12404..12946)
                     /locus_tag="ACHGMT_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFN6835486.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(12951..13178)
                     /locus_tag="ACHGMT_001747"
     CDS             complement(12951..13178)
                     /locus_tag="ACHGMT_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFN6835487.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(13257..15137)
                     /locus_tag="ACHGMT_001748"
     CDS             complement(13257..15137)
                     /locus_tag="ACHGMT_001748"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFN6835488.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(15326..15940)
                     /locus_tag="ACHGMT_001749"
     CDS             complement(15326..15940)
                     /locus_tag="ACHGMT_001749"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6835489.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            16082..16642
                     /locus_tag="ACHGMT_001750"
     CDS             16082..16642
                     /locus_tag="ACHGMT_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN6835490.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(16947..17261)
                     /locus_tag="ACHGMT_001751"
                     /pseudo
     CDS             complement(16947..17261)
                     /locus_tag="ACHGMT_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
BASE COUNT         5575 a         3282 c         2700 g         5907 t
ORIGIN      
        1 tattttaaaa aataaacata aatatgcagt taatgtaaca agataagaaa taatagtcgt
       61 tactgtataa cttgtgctta ctttaatatc agcaactcct ttgactccgc ttatttgtac
      121 caatccatta gcattcacct gctcctttac aggttgatga tttatttcta cattcgttcc
      181 taaataagca tatacaggaa gtgtgattac ttggctcttc tcattagtta ctctatatat
      241 gatagaacta ccagttgttt tataaggaag gtttacccat tgactgccaa tctttgcttc
      301 gtgttttatt atatgctcct tattctccaa tgaagcgata ggagaataat catataaaaa
      361 ataatccatt cctttagtgg tatacgcctc tactgtttct ttgtttacaa caatatgtcc
      421 cggctgagaa attatttttt gattgaaatt ttttttacta gaaacagtta aagtgattaa
      481 taacaaaaca gtcataaata aataaaatag ttctcttgtc ttattgtatc ttctattttt
      541 gaaaaatatc atcgttaata tcagagaaga gaatattatt tgcaagctta agatccgcca
      601 aggaaattgt aacaatttgg ctggcgtatt ttgcagaaga taccatggca ccaaactagt
      661 tgtagctagc caagtcaaac acgtaccata ataaatcttt ttttctatgt ttgttaattt
      721 tttaaataaa atcaggggaa tacataaaga taacagacca actagtccca ttgagtaagt
      781 agttaagtcg ttgttcatag cttcagtcag actatcgaac acattagatg catgagcata
      841 taaatttgtc acgctaggtt tgttgatttt ttggtagagc atttgttcaa acatcggata
      901 ccaaaaagca ctcgtcagaa ccaatgtcat tcctgccgca ctaaataatt ttttaaatat
      961 agcaataaaa tctgcattct tgaaaccttt agaaaccaaa cgaataaaaa taaaaatgat
     1021 aattaatgct gtactcatta atgttgatag tacatggctg tacactagca aactcatact
     1081 caatgctagc tttacagacg gatacttttc ttcttttcca gagatgattt gataagcata
     1141 aaataataca gggataacaa gtgttattgc gatagcttcg gcaattgcgg aacgatagta
     1201 gatgtctact gtccgataag cagagaaaat atagagacaa ctaaataaaa atgccgcttt
     1261 tttttgtttt aaaaaccgct cgccataatg ataagctacg agtgttgtac acaagttcaa
     1321 taaccaaacg taaataatat agcttacaat caaatttttt gtcatccaat ataacattac
     1381 agctggaaaa aaagtcagaa agggataaaa ataatttaca ccatatccag tataattgaa
     1441 tgtctcataa ttaatcggac tggtaaaaat agtcgataac cccttgattc gagataagtg
     1501 aaaagaaaga tctccagtgg tataaatact ctgtgtattt ttgaaaaaga tgaaagaata
     1561 tatactacta actataatta atccactcaa tattactact tttttattta ttttcattct
     1621 tcttgtttat ctccttttta gtcacatatc gagaatcttt caacttatta ggtgcaaatc
     1681 gtttaaaact tgaatacacc actaaacaat aagtcagaat actgatccat aaagccatga
     1741 gagcagttgg agtatatttt gttgtaattt ctatcttatt ttctccagca tttacatcca
     1801 cattcactaa gccatgtttt ttataagagt catttacgac tttaccgttt acttttacct
     1861 gtgtacctaa ataagcatat actggtactg tcactttttg ctttttgttg cttgccactg
     1921 tgtaagtaat gttattatct gcagctttat acttcccttt ttcccacgat tctcctactt
     1981 ttacttcatg tttttctaag tgaggccttt cttttatcgc ttctgttggg gcgtaatcgt
     2041 ataatcctcc catatctgat gttgtataac gtgggagtgt ttcttcattg ataacaatat
     2101 gaccgtgttt tgattggata acttttgcgt aattttcttt tgctgctata gacgtgacga
     2161 gtagaaacac cacagtagcg ccaatgatta accatgaata ttttttattc atagtctttt
     2221 tcataaaaac aagcgttatg atcattgaag aaaaaaagat ttggatacct aagatacgcc
     2281 aaggaaactg gagtaagctt atcggtgttt tttgaaacaa aaaccatgga aataaattag
     2341 ttacgcatac ccatgtagaa actacgccaa catagatgat tttttctttt ttctccattt
     2401 ctttcagtat gaataaagga aaaatcaacg caaccaatcc taataacccc atagtgtatg
     2461 ttgtaatatc gttatttaac gcttgaatga tactatctga aatagaaagt gcttctagct
     2521 gcaaatctgt tttgaaggga ctgtgtattt cttgataatt catctgctgc aacataggat
     2581 accaaaaata gctagttaat gcacaagtca aaaaaacagc ctttactaaa ttcacgacca
     2641 caaattttat atcgtttttt atttgcttgc ttaaaaacaa acgaactata atcatacaag
     2701 caatcaatgc actacacaat agtgtagtta agacatgagt atacacaagt agacttatac
     2761 tgacagataa agcgatgact tttttttctc tattgaatat caatccataa gcataatata
     2821 aaacagctgg gataatcgta agagcgattg cttctgcaat tgcggatcga taatagatat
     2881 caactgttcg ataagttgca aaagtatata aacaactaaa taaaaaagca gcatctatct
     2941 ttttaaaaaa cttttctcca taataaaaac taatcagaat cgtacaaata tttaataacc
     3001 aaatatatag aacataactt aatattaagt tatttgaaat tccataaaaa acgactgcag
     3061 gaattattgt aagaaaagga taaaaatagt tcagtccatc tccataattg ttaaatgttg
     3121 tatagttgat tggcccttca aaaatagaag ataacccttt gattcgtgaa agatgaaaag
     3181 ataaatcacc tgttgtataa atttcttgag catttctcat aaaatttact gaatataaag
     3241 tactaagtaa gataagaact actaaaagaa atgctttttt acttattttc atagatttgt
     3301 tgttctcctt ttttaattct attttcagta tactagaatt gtcttttttt aataagtttt
     3361 gcgttaaatt tttcatagca ctagtatgat atactagaaa agaatcaatt tttattactt
     3421 ttaaatagga gaaaaaaatg aaaaaaaaca aaaaccaaat aatagatatc atttttatgt
     3481 tatttctaag ttttgcttac gtcatccctc tattcatgtc taagggaatc tatcatagtg
     3541 tcaatcagga tacttacttt catttatctc gaatcatagg actagataat gtatggagtt
     3601 ctccagtaaa cttcaataac ttcgatcatc atggaacaat gatgaatatt ttttatccat
     3661 ggcttactct ttatccagct tttttatttt ataaaatgat gggaaatctt gttttaagtt
     3721 ataatatcta ctattttttt atcacttttt taacaatggt cgtttcttat ttttctatga
     3781 aacaaataaa aaacaatcgt tatatttcgc tgttattttc gattatttat acattttccg
     3841 cctatcgtgc cattgacata ttccatcgtg cttcattggg agaagctgtc gctttaacat
     3901 ttttgccttt gatactaatg gggtgttacg aaatttatat tagagattac caaaaatggt
     3961 actggttgtc tattggcatg acgctagtag tgtatactca tttactttct gtcgctatgg
     4021 tttctgtatt tattggagga acgttgtttc tttcttttta tttttgggac caaaaaatcg
     4081 ctagactctt aagtctttta aaagctaccg tcctcacctt ttttttaagt gcgggattct
     4141 taattccttt tattcaacaa tctagagcac aagaattgaa agtcccactg ggaaaagaat
     4201 taagtggtat ggctcctagt gatatgctga cacatatttt aaataataac tataacaact
     4261 atactattgg attattctta tttctcggat tgattggtgc ttttattttt ataaaaaaat
     4321 taactgctga tgatttattt attttctttc ttggtgtatt tgttcttttt tgttcaacca
     4381 atttatttcc ttggcaactt tttaaccata caccagttaa gagtctacaa tttgtctggc
     4441 ggttaaatgg tttttcttct ttatttatag cctatacgat gagtatagtg atctattata
     4501 ttttttctac taaaaataat tcatggaaaa taatggtctt tacgtttctt gcactaatat
     4561 tgcatctctc aggagtaagc aattctatcc atgctaacaa agattcatta acacttatag
     4621 cacctgaaga cgctgttgct atagctgaaa attacaatca tactgattat gcaaacaaag
     4681 agtcaatcta tcatcctgat atgattaata atgatcaata tttactaggt aaccaggaaa
     4741 taaatccaac aactcatttt atgagtaaca aacttacaat tgaattagat aattcgaata
     4801 ataaaaatac tgtcttgacg acacctattt atcgttataa agggcaagtg gcttctatta
     4861 atggcaaact agtacaaaca aaattatcta agtttggcac aactgaatta actattcctc
     4921 caggtataaa taaagtcgtt attacttatc agtacacaaa actagcaatt gcttcaagat
     4981 atttatctat cgttactttg attttatttt tactttatcg atttactttt tctaaacaaa
     5041 aacaaccgag aagagaagta caacattctc actagatttt tatttaagaa agacttttta
     5101 gctgtacaca taaacaggct aaaaagtctt tttttatatt tctttaattt gcttcataca
     5161 ttatttaaca tcgatattta tactgtgaaa catgcgtttc atccattatc aaagtatttt
     5221 tatcgataat aaacttcatc caaaaacaga ccttgtgcag ggacggtttc tcctgcatgt
     5281 tgccgcactt tgctttcaaa tatttcatcg attgtttcta tcggcatggc acctgtgccg
     5341 atttctagta atgtccccat gataatccgc accattttat ataagaatcc gtctccgaca
     5401 aaagtaaaat gaagcatttc tccctttcgt tcgatagata attcatcaat cgtacgcact
     5461 gtggattttt ttgatttttt caatgcagaa aaaccaatga aatcatgtgt acccactaat
     5521 ttttcgcaag ctgcattcag cttgtccata tccaactctt gagggaaata aaagctgtgg
     5581 tttcgctcaa atgctgaagg aatcacgttg ttccaaacat aatagctgta ttttttgccc
     5641 accgcattat atcttgaatg gaagcgctca ggcatttctt cgattctttt gatgacaata
     5701 tcccgcggaa ggtaacgatt cataaagtcg atcatttctg ccagtccaat ttctgtgttt
     5761 gttttaaaat ttgctacttg tcctcgcgca tgggtgccag catctgttct tcctgatcca
     5821 ataatttcta tctcttcatt tgtcatctga tgcaatactt tttcgatttt tccttgaatg
     5881 gttttatcgt catctccaag tctttgccat cctgaatatc tttttccgtc atattcgatt
     5941 gtcaacttga tgtttctcat attttagtgg ctcctctgtc ttccatttgg tcttctctct
     6001 acctttttaa gattgtagca caaaatatag ctccttgctt ctctaagaca gaagctatac
     6061 aagaaaactg tggaaatata tatttcagaa aaacaaaaaa cagccaccgg atgaagtatc
     6121 cgctgactgt tcaatttgac ggctcaagtt ttactgtttg agatgcaaga tactcaacca
     6181 ggcaatgggc acataattaa agaaaaaact gattctttgg caaaaaccta ttttattcag
     6241 taatggccaa gaatttatag aaggaattct tgccagtcca taaatcgccg caaaaagcaa
     6301 actgatcaca gacagtccaa ggtataagtg gcttacgtcc atttgcccat tttttcgata
     6361 gatcaagaaa agaagaactg gaaacaataa aaaaccactg taacctaaac ctgatccgat
     6421 attatggacc catgtcgaga cattccacga agattcatgg gtattgatac tgaacaaacc
     6481 tgtaaagata caatcaccta taccatagaa cgcgatggct gctgtaacta gggtagctaa
     6541 cacacgagat gtactaacaa atgcagcata caatgcaggc aatgacagga tgaaaaagat
     6601 ccctgaaacc acagaccaaa taagaaacgc ttttcttaca ggactggaaa cctctccaaa
     6661 tacactgatc acgctcttca tttgatctaa ttcaggataa tacaatccta atacgtacgg
     6721 agtcaaaaaa tcactcagca cgcctaataa taaaaagaaa aaaccaaatt tacgtaaaaa
     6781 atccatgata cgcctccaat aacaattacc tatgctttta attgttatta taaacgactt
     6841 tatcttgatt taacagaaac ctctatttac acgaacatct atataactca ttaaaaactc
     6901 gaatcatttt aaaaatacta aatatctaac gttcaacgtt cacttaatcc atctaaacaa
     6961 tctttcaatt tgattttttc cccacttctt ttacgcgcgc ctagctctcc tattgcagta
     7021 aacaacagat cacttgacga atttaaggca gtttccattg aatcctggat aacaccgata
     7081 ataaatccaa taccgacgat ttgcatcgcg acatcattag agataccaaa caaactacat
     7141 gctaatggaa tcaataacag tgaaccgccc gcaacacctg aagctccaca agcagctaaa
     7201 gcagacaaaa tacataaaag gactttcaaa acaaaaggtg cttcaatccc taaagaatgt
     7261 acagcagtca acgtcatcaa ggaaatggta atcgcggcac cgcccatatt gatcgtagca
     7321 cctaacggga tcgatatgga ataagactct tttgtcaatt tcaatctttc agccaacatc
     7381 atgtttatcg ggatatttgc ggcagaactt cgggtaaaga aagcgggaag tgcgctttct
     7441 ttcaaacaaa agaaaatcaa tggataggga ttttctttaa taaacaaaaa cgtgaggaat
     7501 gggtacacaa ctaatgcgac aaacagcatc gtaccgatca aaacaagtaa aagctgcaga
     7561 tactctgcca atccagcgat tccagtcgta gcaattgatt gataaaccaa tcctaaaatc
     7621 cctatcggag caaatgcaat aatcacttgt acgacttgtg acaacgctat agatatctga
     7681 tccaccaact cttttgtcga ttctgaacgt gctcgaaaag ctagtcccaa caatacagcc
     7741 cagaataaga cgccaaggta attcgcatca ataagagatt gaacaggatt ttgtgtgaca
     7801 ttcgtcaaca tagtagataa aacactttcc aaactttgcg gtgctttttc agatactgtt
     7861 tcttgtaaag taataggaac agtaaaaatt gaacttacaa taactgctac aagtgcagaa
     7921 agcaatgttc ccactaaata taagatcaaa ataggcttca cgaatgtctt attcccaatt
     7981 ttatgcttgg caattgacgc catcgtcaag aaaaaaacca gaataggtgc gattgccttc
     8041 aagcaactga caaacaacgt tcccaatatt gaaataaatg tccatgtggg gaaaaagata
     8101 ccaaacaata ttccgactaa aataccaaca acaatacgtt gaactaacga catatccaaa
     8161 aacttctcta acaaatgacc accctttcac aattttaaca atcattgtat cattttatgc
     8221 aaagaaacta aaggtataat tttctaaaaa ttctaacaat acaaacaacc atttcttgct
     8281 aattttgtac tttttctgtc tacacgttgt tttttgagat attctaagtt aggatgttat
     8341 taatgggcaa cttatcagga aacgttttct tatgtttttg tccaattttt ttgtgaggtg
     8401 tttattcttg aaaaaaataa agaaggagag ttatggcttc ctgacaatct ttgtcgcttg
     8461 tataatgatg gttttacagc gtatattgat ttcagcagtc actattccta catatgcaac
     8521 aaaccctggt cgattctaca tctatacatt tttacaaact tccgttgtta ttggtgcaaa
     8581 tttgatccca ctttacatgg gttaccaata tcaaaaaatc gcccgtttaa aagttttaca
     8641 ttatttaagt cgttttgcct ttatattcct aatagccaca ctagtttgca atatcttttt
     8701 ttacgtacaa gctggcagct tgaatattcg tgattattgg cttatcctaa cacctatcag
     8761 tcagaattac tttacttatg ctgtttcttg tactttgctt ttttgtagta ttccttatac
     8821 agttggattt ttaaataaat tatcgaaaac aacaattaaa tatctactat tatttttaac
     8881 aattggatta gttggctgct ctactctatt taataaagac ccgtttgcat tacagaatgg
     8941 acagagtatt ttatggattt tttatttatt tttaatgggt tatggtttga aagaatggaa
     9001 ctggaaagaa aaggtccgct ataagcttcc tcaagtattt atcagcttga ttgttttatt
     9061 cggtttgatt attttgatga cacaaatttc actcatcatc agaggaaata cagatacagc
     9121 gctgaggttt tctaaaccat attctctctt cgcaatgtat tatactttct cttcgttttt
     9181 atttttagaa atgctatctg ataaattagg tataaaagga agaggaaatt ttcttgctgt
     9241 atttttgatt gtcactcaag ttatatgtaa tttcccttta gtttcttatc gcgtatctac
     9301 atttatgaaa cgtgaatttc caaactcagg gaaggcatgg ctgataaata tcgggcagtt
     9361 ttttatttga tatttattag cagtgtccct cctagttatc tttttatttt atatacaaaa
     9421 aatccgtttc attcatcgtt tcatcgaata cttcgctttt gattcgattg aacaattaac
     9481 tcagcgttgg aaaaaaagaa ttcattggct ttttgttcat aaaagttatt ttttagttgc
     9541 agcatttttt tactgcttta ccttcgtgca gattttcgtt ttagaaccaa aagaaggttg
     9601 gaaagaaact atccaggttg ccttaaaaat atttactcag cgacaagcac ccatgatcct
     9661 gacaatcatc attattatgg gatttttctt cttactattg ttgatttcca atcgtttttg
     9721 gtacgccttg actgctacct tgatcattaa cttactgctc actatttcga cagtaatcaa
     9781 aatggaaatg cgggaagagc ctgtctttcc ttcagattta aaaatgctga ccggattgtc
     9841 agaactatta tcgatggtta gcccagtctt attaatcgtg ggcggattga tccttctcct
     9901 attattgatt actagtatta ttgtacaacg tagactacaa aaacagtatt ccttgaagat
     9961 tcattggaaa cgtcgattta tcagtatcgc tgtattatta ggatgttttt cgggtgtctt
    10021 tttcattaat cacaaaaatt cgccttcttt cttgctgttt aatttattta aagtaaacaa
    10081 gactttcttt aaccagcaag gcgccgtaaa agataacgga ccagtcattc aattcctaaa
    10141 caatatcgac attaaaatca tggaagaacc tgctggctat tcgaaagaaa aaattaatgg
    10201 aataatgaaa aagtacaaca caaaggcaga tgaaatcaac caaacaagaa gtgactggtt
    10261 aaacaatcag acgatcgttc tcaatttaag tgaaagcttc agtgatcctg cacgtgtccc
    10321 taatctgaca gtacctacag accctattcc ttacatcaaa agcattataa cagaaggaac
    10381 aggaggattg atgttatccg tcggttatgg tggcggaaca gctaacatgg aatgggaagg
    10441 actgacaggc ttgagtatca gtaacctgtc tgcttcgtta gttacacctt atacccagct
    10501 agtggagcgt cagaaaataa gtccaaatat taccgatcta ttcgatgaaa aaatagccat
    10561 ccatcctttt acagctgctc tttatcgcag aaaacaagtg tttgataaat ttggttttga
    10621 caggttctat tatttagaca gtccggataa attaacttat acggataaaa tagaacaaag
    10681 cccacgcata tcggatgatt cagcatacaa agaaaccttg aaagctttac aaagcaatac
    10741 ggaaacgtca caattcatcc aattatccac tatgcaaaac catatgcctt atgatgatta
    10801 ctatagtgaa ttgaactaca ctgctgaagg aactgctgtc attcctaata gaagaaaaga
    10861 attacaaact tatatgcaag gccttcacta taccgataca gcagtaaaaa aattcatcca
    10921 agaaatcgac aaaatcgata aaccgattac ctttgttttt tatggtgacc atttacccgc
    10981 aatctatagt gggaatgatt cgaaaaaata tggattagaa cagcaccaga cagattattt
    11041 catttataac aacaagtata gtcgtgaaca agcagctacg ttaaataaag aagttgttgc
    11101 tcctaataac tttggtgcta tggctcttga gcatgcgaat atcaaagtaa ctccttacta
    11161 tgcgttgttg acagaagtat ctaatcatct acctgcatta acaattgatc ctacagaaag
    11221 cttgtctaat cgttttaatg gaaaacaggt ctttgttaca gaggatagtc aaattatcca
    11281 agaaaaaaat ttaactgaga aacaaaaaga aattctagaa gattatcgtc tgatacaata
    11341 tgatcttgtt gcaggtaacc agtacagtgc aacatgggcg acacaaaaaa taaaagacta
    11401 acaaataaac tgagcaaggg gttgtgataa aagacatgtc acaacccctt gtttatctca
    11461 ttaatttgct tgtccataaa cgatttgatt aaagccattt ttcaattcat tattcgctgt
    11521 ctcgttgaag taaagataat tatcgggatt cccctgattt tgttgtgtca ccaatacttt
    11581 tggctgattt tgctgaaacc cgaatagata gacgtgcttt ttcagatatg gttctgtctc
    11641 tgcatcggaa tagacggctg tgatttgata gtctgcttga cctgtcccac tttctgacca
    11701 ttctgcagaa acaggcactc caccaatagc catcttccac tccccgttca aaatagcttg
    11761 agggacttta agaccatata agtccacact catttgattc gtataacttt tgtattgttg
    11821 tcccattgtt tggccccatt tggtcataaa tgcctctaat tgtgcggatt tgtctgcatt
    11881 ccacagtgtt tgttcaactt gttcgctgct ttctgaagag gaagatgaag cctctacaga
    11941 agaacttgaa gtagatgttt gagttgtttc agtcgttttg gaagactctg acgaagtaga
    12001 ggtatctgct gtttctgtac ttgctgcagt tgacttgctc tctgtgctag ttgttgtagt
    12061 agttgcaact gttgctttcg ctgtttcttt tgttgattct tttttcgatt cattcccgtt
    12121 ggtacaacca gctaacaaaa aagctagacc aattgttact aaaatccttt ttttcaatta
    12181 aaaaactcct ttcgattact cccacttctt tattctatca aataagcaat gtgattttat
    12241 tgatttttcg tattttctca tcaatctttc aaatcttcta aaaactgaga agcacccgct
    12301 gatgttgtct gtaatacatc agcgggtgct tcttttattt taatcttcgt gattttccaa
    12361 tttacctcac gctttatatt tgtatttgaa gaaacataga cttttagtca tcatcgtctt
    12421 cctcatccaa atcttgtcgg acagtttttc cttggagtct ttgtacttgg tagatgattt
    12481 ctttattgaa acttaaaagt tcaacaagca attgttgcaa gccatatttt tcttcttttt
    12541 cagctaattt gattgcccgt gtgataaaga cattttggaa atcaaaatct tttgcaaagt
    12601 tttccaacat tgttgaggct ggtaaatatt tgttttgacc atgttcgtaa atttttccat
    12661 aacgcatgac ttcttctaaa gtagacggaa cgatttcatt ttcaatcact aactcatgag
    12721 agattttatt gaaccatctc gcttcttgat cagcatattc tgaaaaaagg gtacttaatt
    12781 cttgcgaaga tgtacccaaa acatacaatt gtgcttggat caatttcatt tcttctaatt
    12841 taaaatttgc taatacatgc cccaccatgg cgccagcagt tggtttatga tggtcgatat
    12901 ctgcttgttt tgtttcttga acaaattttt cttccactat attcattgat ttacgcctct
    12961 tttacttttg ctgattggac ttcataacct aatttttcca caactgcagc taattgttca
    13021 gggtggactt catctggatc aaagtttgct ttgattttgc tcgcattaaa caaaactttc
    13081 gtattgctta ccccaggctg ttgagaaacc gcatgttcga ttttttgcat acagcttgga
    13141 caagctagtg tatctaattg cataataatt tttttcatcg tagttcttcc ttcctttttc
    13201 ttgatagtct tattctagat tacagcttcc ataaaaaaat tgattcttat caagttttac
    13261 gatttttctt ctttgctttc gtgtccaaaa tacaataaac gcatcgcgtt aaagataacg
    13321 atcaagatac tagcttcgtg aacgaacatc ccactagcca tgtaaataaa cccggcaaat
    13381 aagccgttga gtaaaaataa gacgacacca atggcaatca ctacattttc acgggtattt
    13441 aaaacagttt tcttagccaa gcggtaggca tgaaccaacg attcaaaatt agattgcatc
    13501 aagacaacgt cactcgtttc gatagcaaca tccgttcctg atcccatagc aatcccgata
    13561 tcagccgcag ccaaagaggg agaatcattg attccatctc caataaaagc aacgcgcaat
    13621 ccttgttctt ggtatttttt cacaaattga accttttcgt ctggtaataa ttcagcatgg
    13681 acttcatcaa tgcctaacat atcagctaca taatcagcag tcatctgatt atcacctgtc
    13741 agcatcacta actgttttgc tcctgcttgt ttcaacttag ctaattgttc agcgacttct
    13801 ggtcgaatca catctgacac acctatgatt tgagtgattt ttttgtcgat tgctacaata
    13861 atgaccgagc tgcctgtttg ttgtaattga tgtaaatctt gtacttgctt tggtgtgaga
    13921 gaaagtttgt gagcggcaat caatttggtg ttgcccgcac aaactgtatg accatcaatc
    13981 tcagctgtga ttccttgtcc cttgattgtc tgattatctt tgactgctaa ttgttgaaaa
    14041 tcaatgtctt tctcctgagc ataatggatc acggcgcgcc ccagaggatg atcggataac
    14101 gtttctaatc gagccactaa agctaagctg tccccttcat cctctgtata gttcttgata
    14161 gtggatacag cagtagttcc tttggtcaat gtaccagttt tatcaaagac aaaagtatcg
    14221 attttggaaa aagtatccat tgcttcaccg cctttgacta agatgccccg tttcgctcca
    14281 ttaccgattc ctgctacatt cgaaacaggc gcaccaatga ctaaagcgcc aggacaacca
    14341 agaaccaaaa cagtgattgc taaacgaaaa tcacgactaa tcagaccaac aaccaacgca
    14401 ataagtaaaa cggttggagt ataatactga gaaaatcgat caatgaattt ttccgcatgg
    14461 gatttggtat cttgtgcttc ttctactaat tcgataattt tggcaaaggt cgtatcatct
    14521 ccgaccttag ttgcttcaat cgtcacgtag ccatctgcta aaatcgttcc agagaagact
    14581 tcatctccac tgattttgac gacttgtcgt gattcaccag tcactgcggc ttcatccgca
    14641 taccctttcc cgtcaatgac tcgtccgtcc acaggaatag ctccaccagt ttttactaag
    14701 acgatatcgc cttcttccac gtcatcgatt tccacttctt cagtcgttcc atcttcttgg
    14761 acaacactag cagtagatgg ggccatctcg gtcaaagcct tgattgactc ccgcgttttg
    14821 gtcaatgttt tttgttccag atagctacca aacaaaaaca agaatgtaac gatagctgac
    14881 tccttgtatt ctccgatcat aaaggcacca agcacagcaa tagtaacgag taaatcaatt
    14941 gagataactt tgactttcac tgcctgatac gcatggatca taataggtat gaccgcaata
    15001 atagaagcta tggctagagc aatgttatag aacaagacaa aaccagtaaa ttttccgata
    15061 aaaccaaaaa taatcaacac agctgataag gcggtgatat gatttcgatg ctcttggatc
    15121 cacttttgta atttcatctt tctccctctt ttcctttcat tcttacgaga ctaagtatag
    15181 atacttatcc atcataaaaa attgaccact atcaagttta gtcaaaaagg cggttttctt
    15241 ttggtagaat acactacaaa aaatagttag caagaagact cactaaaagt gatcttgcca
    15301 actattgtcg tttctatcga aattttcatt gaatgatttt cctgtacagc atacatcgga
    15361 gatctatttc tttatctaag atttttatct tgctaatttt ttcctttagt ctttcaaatc
    15421 cacgtcgttc atagaattga tacgtacagc cactatctgt aaaaaggaat atttcttttc
    15481 ctttctctct tttttcaaat tctgttagca acttggtacc gatgcctttt gttttcaaat
    15541 caggatttgc agctaaaaaa atgatctctc catctggcga atgcttcttt aaatatgctg
    15601 caagcatttg ttcatttgct gtctcgtaag ctcctttact ttctttagca aatatatttt
    15661 gtaaaaaatc gaatatctta acatatattt gctgtccaaa agaacgatgt ttcttttttt
    15721 ctccttttat ctccgccagc aaaacacctg ccaactcgtc ctctgcatac aaagcaagaa
    15781 tttgagttgc tcgattcaac tccatataca aaaaatatct tccgtaaaga ttcagcagaa
    15841 gttcattttc catatatctc tcaaagttca ttccttcaac agcaaaatag atagcttttt
    15901 tgtaatcttt cttttctaag ggtttaaaag ttagtttcat catttcgcct cctcaacttc
    15961 aatgattgtg ttatacttac attataatag acacagtgtc tactatctat aatcaatcat
    16021 ctataagttt gtcgattaag cgacagcttt gctttgatat gtcggataag gagataggta
    16081 aatgaaaaag acagatagac gcgtcaaaaa aacagaaaaa gctttggctg aaacactatc
    16141 cacattatta gtaaataaaa agattcaagc aatcactata cgcgaactaa cagaaacggc
    16201 agatgtacat cgttcgactt tttatacaca ttataaggat atctatgatc tatatgatca
    16261 attagaaagc aacttttttc gggatctaaa tgaaattctc tcttatgatc cagcccatag
    16321 ctatgaggaa ctatacactc gcttgattga ctatgtttac gcaaatactt ttttatatcg
    16381 cttatttatc ctcaaccatg gtgatatttc gtttcagaag aaagttaaac aagttattga
    16441 agaaaactat ctgaacatat ggctatatga agacaagaaa acgattatca cagaagaaat
    16501 gagattcttt actacgtacc acgtccaagg ttgtctatcc gttctcaatc ggtgggtaga
    16561 aagcaattac aagtactcca aaaaagaatt attagaactc ttacagaaac tgaatgatca
    16621 catagagaaa atcatgcctt gaataaaaac atactaagct tagttaagaa gaaccgtaac
    16681 cttctcgtat aacaactgaa tcttatttag ccaaattgtt tactgaagat tttttgagta
    16741 aaatttctat aattcctact tattgaaaag tagtttgtag tgctgcttct ccctctctaa
    16801 gtgatatatc ttgttgtacc aatcaaattc acctcatcat taataaatta cttttaggct
    16861 aatttattac aacttatagg ttgaagctgt aaccaattta tagtcatatt atatttttat
    16921 catccagttt attgaattct tctcgtttac ctcaattcat tttcaagggg aatcagctca
    16981 tcatctaaca ttttacgcca tgctcgcttc atttcttgtg ggtatttaaa tggtttcgct
    17041 tctggatgtt tggctttata tccttcgtaa tccttactgt tgataattgc tttcatatta
    17101 ggaattggat cttcacctgg catatattgc agattagaag catgtaattt tttctcagaa
    17161 acaaaccact cattggaaaa gacatgaatc aaatcatcac ttacttgtgt aaaatagcga
    17221 cgtttaatga caaagatatc ttcattgcta tctagtgtac cttcatttct ttgtgcttaa
    17281 attcaaagat aattttctca ataaagattt ttgattccag taagattcct ttactccttt
    17341 tacaacatag cgttgtcctt tgttttgaaa catcttcttc ccctctttgc cattaaaaat
    17401 agcccaattc tctataaaga agattaagct tcattattat aatataaagt ttaaatttgg
    17461 ttta
//
LOCUS       DAZBSA010000057        17218 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000057 DAZBSA010000000
VERSION     DAZBSA010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.057"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..1124)
                     /locus_tag="ACHGMT_001752"
     CDS             complement(105..1124)
                     /locus_tag="ACHGMT_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN6835491.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(1146..2153)
                     /locus_tag="ACHGMT_001753"
     CDS             complement(1146..2153)
                     /locus_tag="ACHGMT_001753"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFN6835492.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(2146..2811)
                     /gene="pgmB"
                     /locus_tag="ACHGMT_001754"
     CDS             complement(2146..2811)
                     /gene="pgmB"
                     /locus_tag="ACHGMT_001754"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFN6835493.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(2804..5098)
                     /locus_tag="ACHGMT_001755"
     CDS             complement(2804..5098)
                     /locus_tag="ACHGMT_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFN6835494.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5358..7523
                     /locus_tag="ACHGMT_001756"
     CDS             5358..7523
                     /locus_tag="ACHGMT_001756"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFN6835495.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7827..8660
                     /locus_tag="ACHGMT_001757"
     CDS             7827..8660
                     /locus_tag="ACHGMT_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFN6835496.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(8762..10462)
                     /locus_tag="ACHGMT_001758"
     CDS             complement(8762..10462)
                     /locus_tag="ACHGMT_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835497.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            10969..12129
                     /locus_tag="ACHGMT_001759"
     CDS             10969..12129
                     /locus_tag="ACHGMT_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFN6835498.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            12145..12588
                     /locus_tag="ACHGMT_001760"
     CDS             12145..12588
                     /locus_tag="ACHGMT_001760"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6835499.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(13071..13601)
                     /locus_tag="ACHGMT_001761"
     CDS             complement(13071..13601)
                     /locus_tag="ACHGMT_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFN6835500.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            13706..14257
                     /locus_tag="ACHGMT_001762"
     CDS             13706..14257
                     /locus_tag="ACHGMT_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFN6835501.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(14410..14883)
                     /locus_tag="ACHGMT_001763"
     CDS             complement(14410..14883)
                     /locus_tag="ACHGMT_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFN6835502.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            15021..16493
                     /locus_tag="ACHGMT_001764"
     CDS             15021..16493
                     /locus_tag="ACHGMT_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFN6835503.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            16490..17200
                     /locus_tag="ACHGMT_001765"
     CDS             16490..17200
                     /locus_tag="ACHGMT_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFN6835504.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         5351 a         3098 c         3536 g         5233 t
ORIGIN      
        1 cacgctcttt tttacagata aacaatgtgt tagaagtagt atcttctttt acataaaaag
       61 ctgaccatag gataaatggt cagcgtattt agtgagtcag ctatttagta tgggttttct
      121 aaaggaacga ctgtctctct tttgatcaag cgatgaggga tgaccaaacg tacgccactg
      181 ctcaattctt catggatcaa ttcgcgcagc ttttgcatag cgacacgtcc taattcggag
      241 atatcgatat cgatacttgt aaggtaaggg tgtgttaatg ttgagaagat cgagttgttg
      301 aagctaatga cggagagtgt ctctggaatg acatagccat acatttgcgc caactgcatc
      361 gtgcgaaggg cgaagatatc gtcaatgacc accaatgctg tggcttcgtt ttctttcaac
      421 gttttctcaa aggacagata atcctccgga tgctctagta ctacactgga taacgcagga
      481 agaccagcca tcatcattgc tttttgataa ccgaaatatc gttcgaaata caaactttca
      541 tgagtcgtgt ttgtcacaaa caggatattc ttatgtccat tctggatcaa aaattctgtt
      601 gcttgtttac ctagaagctg gttatcatta tctacataaa cgatttcgtt ctcatgatga
      661 taaggctgtc cgatcaaagt gaaagggatg tggttttggt ataaataatc aatgacagta
      721 tcttgactat ctgaatacac tagaataaaa ccatctactt gtttttgtaa atgcatacgc
      781 tggacgttct ctaacagcaa ttcaaaattt ttagcagtag cgattgctgt agtcatatca
      841 taattccgcg cttcttcgtt gattgcttcc atgatctcaa gataaaatgg gttaccgatc
      901 cgctcttttg aatcaagagg gggtaagatt acgccgatgg cactggaaat gcgttttcct
      961 agattgcggg cagtcatgtt aggaacataa cctagttcat ccataatttt tcggactttc
     1021 ttctttgttg tctcggaaat actaggatga tcgttgatga cacgagagac ggtcgaggtg
     1081 gcaacaccgg cttttttcgc cacatctttt acggtaatag ccatgataaa aacacctttc
     1141 tcttgttatt gtgcagagaa ccgataaatc gtctgactga tccgttcttg accaggatgg
     1201 aggttgatcg atccaaattt ggggaaatga acgatatcag ggatttcttg cggttcgatt
     1261 gctaatccgt agtttgaatg catctgtttt ccattgacag agaaatcagc ttcgaatcca
     1321 gtcgtagaaa ataataccat acttttatta gaagttgcta tagtcatttg gcgaccagaa
     1381 tgcttctcga ataattgtaa ggaagttttg gaaagcctag gatgatgcaa aagaaataca
     1441 tcatctagtc catctggata acatgcaagg atctcttgca gtgttgggga ttttctaaag
     1501 tcgattggca gtttttcttt ttttgtccaa tttccagtag gcagtttttc ttgatctaat
     1561 tcaagcacac catctaaaaa gaccgttaac tgatgttcat agatgtctct tgttccattt
     1621 cctgaaaggt tgaagtaggc atggttggtc ggattgcaga tggtttcaac aaggctacta
     1681 gcagaggttg tcatctctaa ttcgtttttt gttaaacggt aagtgatcgt agcagtgatt
     1741 ggtcctggat aggtagaaaa ttcatctttt aagtaaaaga ccacacctat tgatgatgga
     1801 gttttgaaaa cttctacttc ccaaaattgg aaagaccagc ctttcgtgcc accgtggata
     1861 tggtgtgatc cgcagttttt ctccaatcta tgctcacccc aagaagcatc ccggatcctt
     1921 ccggctacag gtccgacagt tgctccaaaa aagcttttgt ctgttagaac atcttcgaaa
     1981 cgatcgtaag acaataaaac attttctgat tttccttctt tgtctggtgc gaagatttgg
     2041 tgcaggcggg caccgtaatt gagaagaacg acttctaagt agtcgttctt caatgtgatt
     2101 ttctttattt cagtctctgg aactggtacg acggagatct ctggattatc catggtcatg
     2161 ccacttttct ttcaagtaat cgatcgtaag aaaagtcgta tcagggacaa gcgggatctc
     2221 ctctccaagg tcttcagcac gtcccacgcc aattggttga gcgccacttg agaggatggc
     2281 ttggataccg gcttgcgcgt cttcgatccc gatacattct tctgctttta gtccgacagc
     2341 ttttgctgca aggaggaaga tatctggcgc aggttttccg tttgttgctt ctgccgggtc
     2401 ggcaatagca tcaaagtaag gtgtaagacc cattttttca agtaggaatg gcccgttttt
     2461 acttgcagaa gcaagagcaa tcttgatttt ttcttctttt aaagaatcca gtaaaggcac
     2521 gaccccgggg aagacatctt ttggttcgat cgtctggatc atctctaaat aataatcgtt
     2581 ttttcttttc gctaattctg caaattcttc ctttgaaaaa gagtgttctt ttccgccata
     2641 agctaaaagc aaagagagtg agtcttctcg gctgacgcct tttagctgtt cgttgaatgt
     2701 gtggtcgatc gtaatgccga tttcctgacc aagctttcgc caagcacgat aatggaattc
     2761 agcagtatcg gtaatgacac cgtctaaatc aaacaatacc cctttaaaca tttgcttcct
     2821 cctttcttag cgttatttct agacgatccg ctaaagaata agactgttca tagacgtaaa
     2881 tttctagagg agaaccttct aataactcga aagtaacttg ttcatctgtg actgagatga
     2941 acaataaacg tccgcgataa ttgatatgga aagaataagc tttccaagtt tttggcagaa
     3001 atggtgcgaa tctcaactga tcatgggcag ttttcatttg agcaaatcct tggacaatcg
     3061 acaaccagct accagtcatg gaagtgatgt gcaagccgtc ttctgtgtcg ttattgtaat
     3121 tatccaaatc caagcgagct gttcgctggt acatttctac agctttgctt tccattccta
     3181 attctgctgc taaaatcgaa tggatacttg gcgaaaggga agactcatgg acagtcattg
     3241 gttcatagaa atcaaagttt ttttgctttt cttcttttgt aaaagtatca ttgaagaaat
     3301 aaatcccctg tagtacatct gcctgcttga tgaagcaaga tctaaggatt ttgtcccaag
     3361 accaatgctg attcagagga agatcgcttg tgggaatatc ggatacaggc atgagatctt
     3421 tatctaagaa tgtgtcatgc tggacaaaga ttcctaattc ttcgtcaact gggaagtaca
     3481 tgttctctgc gatatctttc catttggcta gttcttcttc gctgatagtg atagcagctt
     3541 ctttttgata agttaaataa ttttctcgcg tgtaacgcaa gacccaagct gcgatcgtat
     3601 ttgtatacca attgttattg atattgttct catattcgtt tgggccggtc acaccgtgga
     3661 tcatgtattt atttgctcgt tttgaaaaat ggacacggtc tgcccagaag cgtgcgattt
     3721 ctgctaaaac ttccagtcct tcatttttca aatattccgt atcgccggtg taattcgtat
     3781 aattgtagat tgcataggca atcgcgccat ttcggtgaat ctcctcgaat gtgatttccc
     3841 actcgttatg gcattcgaca ccagtgaagg tgaccatagg ataaagcgcg ccttttaatc
     3901 cttgttgctg agcgttgtgg attgcttgcg gcagttgatt atgccgatat ttcagcagat
     3961 tcttagttac ttcaggtttg gctaaagcaa gataaagagg gacagcatac gcttctgtat
     4021 cccagtaagt ggctccgccg tatttttctc ctgtgaatcc tttggggccg atattcaatc
     4081 gttcatcttc gccgtagtaa gtagagaata gttggaacaa attgaatcga ataccttgtt
     4141 gtgcttcttc gtctccttcg atgaccacat cggcaagctt ccatcgttct gcccaagccg
     4201 ctgtttgctc ttcttttgct tggatataat tgtttgtctg gttcgttaat tctgagacag
     4261 ctttttctgc ttgctgcgct tctggaatat ctctactagt aagaacgata atttcttttt
     4321 ccactgacca tgtctcacct tgagataatt ctgcagacaa gcaaccatgt aatgctaaag
     4381 tatcttcgtg atagtcaggg atgactgatt ttccattaac gaaatggcgc attgctgcag
     4441 taacagtaaa ttgttcaatc ccaaagttgt ttggaatagt tttagtcgtc aaatagccga
     4501 tttcttctat agttccacga tcaatctcat tccaaaaatg ttcatcatag ttgctgtctt
     4561 cgttttgaac atttccatcc aattttgata cgagtttgac tgttccgcta ccttcaagca
     4621 tttctatagt taaatagata taagctgatt cttttttgac aatgctcaga aaacgttcga
     4681 aggaaatgcg tactttatta gtagaagtag tgattgtgaa tgtacgagat aatacaccat
     4741 ttttcatatc taattcccag taaaagtctt ctggttccaa agaagccaag tcgatcagct
     4801 gatcgttgat cgaaatatct atcgcgataa agttcaaggc gttgatcact tttccaaagt
     4861 attctggata accatttttc caccagccga cacgtgtctt atcgggatac cagacacccg
     4921 ccaaataagt accttgatgg tggtcccctg agtaagtttc ttcaaagttc ccgcgcatcc
     4981 ccatataacc gtttccaata cttgttaaag attcttgcag tcgaagattt tttttatcta
     5041 attgtgtcgt gctgattttc catggatcaa tagcaaatag tcgtttgatc tgtttcattt
     5101 ttctcctcct aatgagctgt tttatacgtt cataataaac gcaaccgatt gcacaagtca
     5161 atagacaaaa gaaaatattt catagaaaaa agcttaaaaa aataaatttt taggagaaaa
     5221 ttatagaagg gaacacacgt attataggtt ttatagaatg gtatagacag aaaaataaaa
     5281 aataaagcat ttacaaaaaa gaaaacgcac gctataatac gtttaacgaa accgattgcg
     5341 taaaggagct aaacggaatg aaaaagatat ttagttttga gttttggcaa aaatttggaa
     5401 aggctttaat ggttgtggta gcagttatgc cggcagctgg gttgatgatc agtataggga
     5461 aatcaattcc cttgatcaat cctgattgga cgccgcttgt cacaacgggc ggagtaattg
     5521 aaaatattgg ttgggcaatt attggtaatc ttcatatttt atttgcttta gcaataggag
     5581 gaagttgggc aaaagaaaga gcaggcggag catttgcagc tgggatctca tttattttaa
     5641 tcaatcggat cactggggct atctttggtg tcacaagtga catgcttgcg aatgaagatg
     5701 catttaccca cactctgttc ggtacgaaga tcatggttaa aggatttttt acaagtgtat
     5761 tggaagcacc agctttgaat atgggagtat tcgtagggat catcgctgga tttgtcgggg
     5821 caatggctta taataagtac tataattata gaaaattgcc ggatgctttg tcatttttca
     5881 atggaaagcg ctttgttccg tttgttgtta tcttatggtc aacaatcgtg gcattgattc
     5941 ttgctgtggt gtggccaaac gtacaggctg gaatcaataa ttttggttta tggattgcag
     6001 aatcacaaga aagcgcaccg attctagcac cgtttttatt tggaacatta gaacgtttac
     6061 tattgccatt tggattgcat catatgctga cgattcctat caactataca cagcttggcg
     6121 gatcgtatga gattttatca ggcgcacagg cggggacaca agtatttggg caagatccgt
     6181 tatggttggc ttgggccact gatttagtga atcttaaagg tgcaggggat atgtcgcaat
     6241 atgagtttgt gttgcataac tggacgccgg ctcgtttcaa agtaggacaa atgattggtt
     6301 cttcaggtat cttgatggga atggcttttg cgatgtatcg taatgtcgat cctgataaga
     6361 aagcaagata taaatcaatg tatttttcag cagcgttggc tgtctttcta acaggtgtaa
     6421 cagaaccatt ggagttcatg tttatgtttg ctgctgtgcc attatatgtc atttattcag
     6481 tgatccaagg ggctgcattt gcgatggcag atatcttgcc attgcgggtc cattcatttg
     6541 ggaatatcga attgctgact cggacaccgt tagctatcaa agcgggtcta ggtggagatc
     6601 ttatcaactt cgtgttgtgt gtgatcgctt ttggtgttgt tacgtatttc ttagcaaatt
     6661 tcttgatcaa aaaattcaac tttgctacac cgggacgtaa cggaaattac gacaatgaca
     6721 gtgaagaaac cgttgtatct aacagcggaa caggaacagc agatcagcaa gtggtccaga
     6781 tcattcattt gctgggagga aaagaaaata tcaaagatgt cgatgcttgt atgactcggt
     6841 tacgcgtaag tgtaaatgat cgagaaaaag tagggactga agaagcctgg aaacgagcag
     6901 gggcgatggg attgatcgtc aaagacaatg gagtccaagc agtttatgga ccaaaagcag
     6961 atgtcttgaa atcagatatc gaagatctgc tgcaatcagg agcagagatt ccagagccag
     7021 aaatggtttc tacaggaaat ggacaagcag gtagtaagca atacttaggt atagaacaag
     7081 aactagtttc cgcagcttct ggagaagtga ttcctttatc tgaagtaaaa gatccggtat
     7141 tctcacaaaa aatgatgggt gacggctttg ctgttattcc aacatcaaga gaagttgttg
     7201 cgccgatcgc tggaaaagtg acaagtattt tccctagcaa acatgcaatt gggctggaaa
     7261 cgaaagatgg tatcgaagtt ttgatccata tgggaatcga tacagtccaa atgaatcagc
     7321 cagcttttga gattgctgtc aaagaaggcc aagaagtagg aacaggaacg aaattagctg
     7381 agatggatct tgatgtgatc caaaacgaag aaaaagatcc aacgatcatg atcgtattta
     7441 caaacgataa agtagaagaa gtagtgatca agcaattagg tacagtgaca gctggtaaag
     7501 tgatcggctc cattaaaatg taaggagagg ttgtgaaaaa gctgtcctgc atcaagtcat
     7561 aagaacagcc aaacaaaaat agctcctcct atttttcgtt tggttgggct tattaccgag
     7621 aagcagcagt ttgaatactg tttagtggag aggtcgtgaa aaagctgccc tgcaccaagt
     7681 gataaaaaca gccaaacaaa aaatagctcc tggtccattg agcttattac tgaggagtta
     7741 gcaggagaac actatttgct ggagaggttg tgacaaaaat tttgtcacaa cccttttgtt
     7801 tcgaaatgaa gacaaggaga aaaccaatga aattcatgac actcaacacg catagctggc
     7861 ttgagccaga accagagaaa aaattgcaag aattagcaga taagatcctg ttagaagatt
     7921 atgaagtgat tgctttacaa gaaatcaacc aattactgga atctgaagaa gtagaacctg
     7981 cgaagctaat gaagttctgt ccggtaaaga atcaagtacc tatccgtaaa gataattttg
     8041 cctaccgttt agttcagctt ttaaaggaac acggaaaaga gtatttttgg agttgggaaa
     8101 tgagccacat cggttatgat aaatatgaag aaggaaatgc actgcttacg aaaaaaacat
     8161 tggaaagtca ggtccttacc gtctcccaat ttcaaaagaa agaagattat cagacacgca
     8221 agatcttgat tgggaaaaca aagatagatg atcaggacat ttttgtctcg agctgccact
     8281 tttcttggtg gacagataaa aaaagtggct tttattttga gtggaaaaac ttggaaaact
     8341 actttttgga aactcgcgta ccactttttt tcctaggtga tttcaataat ccagtagact
     8401 ctcaaggtta ttatactgta agagaaagtt gccttcttct tcaagattcg tatgttgtcg
     8461 cgaatgaaaa aggaaaagca gctactgtgg aaaagaaaat agatggttgg gaacaaaata
     8521 cagaaaaatt gcgtatagat tttattttcg tccctgaagg aatgcaggtt aaaaaatatc
     8581 aacggatttt tgatgggatc gatagtccaa tcatcagtga tcactacggg gtggaaatcg
     8641 aattagatgt gaacgaataa aaaatataac atataacata aaaaaactga ttaaaataaa
     8701 actctccaaa cgattatacc gtttggagag ttttttgatc ggatgtattt atcccttttg
     8761 cctaagcacg taaaagcttg tatctatgat aaataaataa acatagcaag gtaaaccagc
     8821 caatcaaagt aagagaaaga atgaaataga aatagattgg cgtttgataa tgaagcgtga
     8881 gctcgttgat tcctttttgc tgcttcacga ttggggtccc gatatctgtt acttcataat
     8941 cagtcaatgt ttttccattt tgctgtagca cagtccgatc gtagacgata acagggatat
     9001 gaacagtttt gtccccagtg tttttccact tgattgtcaa aagaccgcct ggatgtgttt
     9061 ttataaaagt tgtttcgttt tctagtattt cttcgttata ccggtcgtat ttattgtctt
     9121 ttgtctcctt atagattggc agatagtcag gtgtggtttt ttggaaaacg aacaagctgg
     9181 tagacatatc tttgctgaag aatgttttcc ttgcttcttc cggcgtatcg aatagataag
     9241 tatgtcgact gacaaatgtt tcattttccc acttttccag atgctggtat aagttctgga
     9301 tcgtctgtgc tgaaaaaata agaatgccaa atatacttaa aagacggaac cacttttgtt
     9361 cagaccactg gttcagtgaa agggcaaggt aaagtagaag caatagcgta aatggcacga
     9421 aaaatcgaaa tggaaactgg atcaaactaa taaaaggcac atttttttcg atcaatgtcg
     9481 tccatggaat caagctagta gaaagaaaaa agaaaacaag acttgtgtac aacacgattt
     9541 tttggaataa ggtgttcttt tgttgaaaga aaataaaaag caaaaaacct gataatatca
     9601 atataggaaa ggccttaggg taatggagcc aataactgcc atccagatct atcgcctttt
     9661 gactcatttc tctgttaaca aagggaggaa gaatcttatt cccactgtaa acaatagcta
     9721 atcctgccca aatatttgct gtcagaacaa aataaagcaa gaccgactta atcagttgaa
     9781 gcagtgcttt tcctttttca gtctggttaa aaaagtaata aagataaaaa gggatataca
     9841 tcaaagctaa catgatcgaa gacagtaaat ggatctgtgt cataagtccc actgagacag
     9901 cgactttgat aacagggaac tttttatgaa ggacgaaatc agcaataggg atcatacata
     9961 aaggataaaa acaagtcccc cagctagtga atccttggtt gatagaccag tattgaatag
    10021 aatacgtact catgaataaa acggctactg gtacacttac ccaatttcga acattgacat
    10081 atctcagtaa aatcagcata gaaaatcctg aaataagata aagaatgaaa tttgaaagaa
    10141 tctgataatg aaaccagcta cctgcaagca acacgatcaa tccttgtaaa taagcgaaaa
    10201 atgggccgta aacggcattc acgatcctgc cactttgctg gaatccataa agggaaggaa
    10261 aataatggaa atccccatat ttaatttgtt gcgcggcatc atagaaccga ttgtaatgga
    10321 atggggcatc agcacccaaa attagtttat gtgtgaataa atgaggcatg agaaatgcca
    10381 aagcaatgaa aaaaattgct aaaaatgggc catatttttt caattgtcga actcctttgc
    10441 aaactttaga taaattcatc atatatccta tgcgagaggt aggcaagaaa ttagaaaatt
    10501 catgaaaata tttatgaatt ttacaagaaa aacataagtg aaagttctaa ggagagagga
    10561 tgcgacataa gttttgttgc atctccttgt tttgaaacag aatttttctt aatactcgaa
    10621 acaaagtagc tttttcatga cctctcggta aacggtgttc aatcagctgc ttctcggtaa
    10681 taagctcact agcctagaaa atatgagaag ctattttctg actagcgctc ttattactca
    10741 aagcataacg cagttttcac aacctcttcc ttaaacggtg ttcaaaagct tgcatctgcg
    10801 gtaataagcc caacccaaca aaaaatatga gtagctattt ttttgggctg tacttattac
    10861 ttgatgcagg acagcttttt cacaacctca tcggctttca ttttctattt ttttcctgaa
    10921 cccatataat aaaagaaaga acaaaaaaac ttgcaaggga ggacacatgt gaagcagagt
    10981 aagcaaaaac agtctcgtcg acttttgttt cgtttgtttt tattaatatg tatcgtcgta
    11041 gggttactga tcgctttata tccattttat gtggattctt taaatagttt tattgatcaa
    11101 aaacgtatcg aacaagtaca aaaacggacc gcagctgaaa ttgaagtgca gcgaaagaaa
    11161 atggaggaga aaaaccaacg attaactgac caaggattca atcctggtac tgatcctttc
    11221 aatgaaaaga acagaaatga aagtacaaca tccagccaat tagaggaatg gctgatcgga
    11281 agtatcaata tacctaagat ccaattaaat atttcacttt atgatcggct caatggtagg
    11341 atccttgaaa atggagcagg cgtcttgcaa ggaacctctt tcccgcttgg tggaaattcg
    11401 acgcattcag tgatctctgc tcacagcggt ttacctaatc gacggttatt taccgaatta
    11461 gatcgtttag aaaacggaga cacgtttatt ttgactgttt tgggggagaa attggcttat
    11521 caagttaaaa atatacaagt ggtactgcca gatgacacgt ctgttctaac gatttcagaa
    11581 ggagaagact tagttacctt gcttacatgt acgccgtata tgatcaacac gcatcgtttg
    11641 ctagtgacag gacatcggat tccttataac gaatcggtaa aaaaagaaga aggaaaagga
    11701 aatcaggaaa gaaccatgcg gcaatggttt attcttgcag gaacgataat agctgtagtc
    11761 atactgttag tgtttatcgg acgactaatc tatcagtata gattaagtaa aaaagtgctt
    11821 gatttttcat ttattattag tgattcgtca gggaaaccgg taaatggtag ttcattcatt
    11881 ttgaaacata agaaaaaaac acttactaga aatggtgtgc cgttttcagt acaaagcgac
    11941 catcatggga aagtcaagct ggatcaattg cctggtggaa cttatcgaat cgtttcagct
    12001 gatgatccta aagttgctgt ttcttttggt attagaaaag taaaacaaga gaagatatac
    12061 ttttttgagg ggcgaaaact tgtaaaagag cttcaaaaaa atggttttta ttttaaaatc
    12121 aatgaataag taaggagaga aaaaatgatc cgcacaacaa caaaattgtc tgccaaagaa
    12181 ctggaatctg ttctatcgat ttggctgcat gctaacttgg attctcatga attcattcca
    12241 tcaacttatt gggagaatca agtagaatct gttaggaaag ctttaccaga tgcagtgatt
    12301 tacttagcag aaatagatca agaaattatt ggttttgctg gggtgattga tcagtttatc
    12361 gcaggaatct ttgtgaaaag ggaataccgg caacaaagaa tcggtacgca actaatgaaa
    12421 aaaataaaag aaaccaataa aacactgact ttgacagtct atcaaaaaaa tcaatcagct
    12481 attcatttct atctttcaca aggttttaca attcaaacac agcaaaaaga tgaagcagta
    12541 aatgaggagg aacttgtgat gaaatggaat caaaacgctg atttttgatg aacagaataa
    12601 cgaaataatg ctcccagcct aattttttgc tgggagcatt atttcatttt gaagtcacta
    12661 atttaactgt gtgaatatgc agttgttaca atctttgata aacggtgttc aatcagctga
    12721 ccctcggtat tagctcaaaa aacggcaaaa tatgaggaac tatttttcca tttgtttctc
    12781 taatactcag gtcaaaacgc tgatttcaca acctcttgat aaacggtgtt caaacggctg
    12841 cttctcggta ataagcccac tagcctagaa aatatgaaaa gctattttct aactagcgtt
    12901 cttattactc aaagcataac gctgatttca caacctcttg ataaacggtg ttcaatcagc
    12961 tgaccctcgg tattagctca aaaaacggca aaacatgagg aactattttt ccgtttcttt
    13021 ctctaatact caggtcagaa cgctgatttc acaacctctc tttcacaacc ttattttact
    13081 cgtttataat tttcatcgaa gtattttccg ctacggatat catctggcat tccatcgtat
    13141 ggttcttcgc taataacatc ttcattgtct atacctgcat cacccatata agtgatcgta
    13201 gcaaattcag gaacaagtaa ttttggataa gtttcatatt tttcacgagc tgcttccatt
    13261 tctgcgatat gttcgttttg gaaagtaaca gtgatttctg gatgttcttc aacccattta
    13321 gggaaaaaga tcattccgtg aagtttcttt tcatttggca taaagtccaa tgccacgtct
    13381 gtccctgttg gctctgtcca agtgattttg aatacgccgt ccgtcaattt aacgatatca
    13441 gcttcttggt cttttaccca acggcctttg accattccgc catggatacg gtaatcaaca
    13501 gtatggtcat ttttggcata ccattcatat tcccaaccat tgtcatacgt atagataaag
    13561 tgagttccaa taaaatcttc caattcttta aattctttca tgtttaagca tctccttata
    13621 aattaaaaca tgtaataaac tacatgtttt cgattactca tttatgatag aatggattta
    13681 aagatttgtc aaggagggaa aactgatggc aagaaaaaat acacttcgat atattttgtt
    13741 agggttatta agtaagaaga agatgacagg atatgaattg aatcaatcgt tcaaaaatga
    13801 aataggagaa ttttggcaag caaaacatag tcagatttat cctgaacttg caaaaatgga
    13861 agagcaagga atcattcagc atcaagtaga gatcacaggt gaaaaattgg aaaagaaagt
    13921 ttatgaatta acagaagaag ggaaggaaca attggcagag tggattcata cacccaccaa
    13981 tgaactaccg gttaatcgag acgaatttgt tctgaaattg tatttcgtca aagacgtgaa
    14041 tgatccggca ttagtagaaa ttattcagca gcagcgtgac cttcatgaag aaaaacgact
    14101 tcatctatta gagcgaaaaa aaacgatctt tcctactgaa aaagaaaaac atgaaaatta
    14161 cggacactac ctgattttag cgcatgcgat caatcgggaa acagaatatg caaattggct
    14221 aacagatgta ttagaagaaa gtaaccgaaa aaaataaaaa aatatccgaa cagtgatgga
    14281 gaattccttt ctatatgaaa gaaacgcaga tcaccgttcg gatattctta tgttcaaaca
    14341 ggcctttttt caagtattta tgtagcatct acttattaaa aagcttggta cttaggagat
    14401 tagtggcgtt tagagttctt ttaacaactg ttcgtagtca gctttttcat cgacaggaag
    14461 gagcttgtaa gttaattcgt ctgtctcgtt gtcatgaatg atgtacatct tagcattctc
    14521 ttctttgaag ttaccgattt gtaaaatcgt atagcgttcg tcgatcaatt cgatatcttt
    14581 gattgctttt gaaaaaactt tacggacatg gtgcattttc ttttggcgga tttctgcctt
    14641 ttctgcagct tttgacaaag taaatccgtc atctaaatat tttttgatca gttctacttt
    14701 tatgactgta ggtaaacgat attttctagg acaattcgca tcagcttcga tcgaacgaat
    14761 gaatcccttt tgttcccaat accttaattg acgtggagac acacctacga tatcacttaa
    14821 ttcactgatg ccaacaacca attgatcatt ttccaacaaa cgtttaaggg aagccccaac
    14881 catttttgtc actccttttg tcctcttgct tcccttaatt atatccgtat taacttatgt
    14941 gtcaagtagg ttgacaaata gcgaatttag gactaaagta ttctagtagt ttcagaaaaa
    15001 attcagaaag aaggagaaat atgagtaaca accaacccgt agatatttac ggaaaaccat
    15061 ataatcggac acttttagtt gttgtcttat taattggtac attttgtacc gttttgaatc
    15121 agaccttatt gacgacagct tttcctacat tgatgaaagc tttcgatatt tcagcgtctg
    15181 atgtgcaatg gttaacgact ggtttcttgc tagtcaacgg gatcatgatc ccaataacag
    15241 catggctgat caataaattc agttcaagaa atttatatct ttctgccatg acgatctttt
    15301 tgatcggaac gattacttgc tttacagcac ctaattttag tacattacta atcggtcgtt
    15361 tgatccaagc ttgcggtgtg ggagtatcga tgccattatt gcaaaatatc atgctatcta
    15421 ttttcccgcc agaaaaaaga ggatcagcaa tggggatgtc agggatcgtt attggacttg
    15481 ctcctgcttt aggccctaca ttatctggtt atatcattga taactaccat tggcgtgact
    15541 tgtttggtat ggtactgcca atcgttgttc ttgtcttggt tttagcattc ttcctaatga
    15601 aaagtgttat tccattgtca aatcctagca ttgatatttt atccgctatt ttatcaacga
    15661 ttggattcgg tagtttactt tacggatttt ctagtgtagg agatgacggc tggggaagct
    15721 tgaaagtaat tggtttctta gttgttggtg tgattttcat cgccttgttc tcatggcgtc
    15781 aattacattt ggaacatcca ttcttggaac ttcgtgtatt caaatcatct acattcacga
    15841 ttgcagctgt tttagctggt gtaacaaata tggcgatggt aggggcagaa atggtcgtac
    15901 cattgtatat tcaaaatatt cgtggagaat cagcattcca ttctggcttg atgttgttgc
    15961 cgggtgcatt gatcatgggg gctatgatgc ctgtgacggg tgcgattttc gataaatacg
    16021 gcgcgaaacg tttagccatc acagggatgt tcattttgac agcggcaact gcaccatttg
    16081 ctttcttgac aaaaacgact ccagttttat atatcgtgat cctttatgcg atccgtatgt
    16141 ttgggatctc catggtcatg atgccggtaa cgacttctgg tatgaatgca ttgccaaata
    16201 acttgatcag tcatgggaca gcggtaaata atacattccg tcaaatcgca agctcgattg
    16261 gtacagctat tttgatcagt gtgttgacga atgtgacaaa agcaaatatc ccaagcaaat
    16321 ctgtattgga taatacgcca ttagcttata aagatcaggc aatcaatgcc acactgtcag
    16381 gttaccatgc cgcgtttatc gtagcgatca tctttgggat tcttggcttt attattgcct
    16441 ttttattgaa gaaaaaagaa cctgttctaa tgaaagcggg ggataatgca tgattattgg
    16501 agttttgatt gtcagcttgc ttgcttttgc tttaacgaac gtgttcgcta aaaaaacatg
    16561 gcaaaccttt ttatctttga ttttcggact aatcttcgtc gcttcactaa gtttgatcgt
    16621 cgctaatttg tccaatcact ttggaatgga aaaagtaacg gaaacaaaaa cagaaaaaat
    16681 tgtttccagt gctgattccc aaggagcaga tatgcttttg tataaagctt tagggaatgg
    16741 aaaagaaaaa gtgtatcttt accgaacaaa tgaaaaacaa gaaaaaccaa aagcaactgg
    16801 tacggataac gaaacaaata aagtagaaaa gacagacggc gatgctgaaa aagtgacgaa
    16861 aactacttat tgggaataca aaaacgacat gtataaattc tggttcaata ttgctgataa
    16921 caatcacgaa tatgacagcc gagtgaatac attcaaaatc ccagagacat gggtagaatt
    16981 aagtacggac caagcagcca agttagctga attagtgaag aaacagcaaa gtacgatgga
    17041 aagtgaagca aaagcttatg ttcaagacgg tatggtaaaa gcaatgactg aaaatccaaa
    17101 aatgagcaaa gcagagcaag aacaacgtac gaaagcctta gctgctgaat tccaacaaca
    17161 agcattcgca aaattagtga aagaagcaaa aggcgaataa tattttgaac accatgta
//
LOCUS       DAZBSA010000058        17201 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000058 DAZBSA010000000
VERSION     DAZBSA010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.058"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            360..1799
                     /locus_tag="ACHGMT_001766"
     CDS             360..1799
                     /locus_tag="ACHGMT_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN6835505.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            2094..2312
                     /locus_tag="ACHGMT_001767"
                     /pseudo
     CDS             2094..2312
                     /locus_tag="ACHGMT_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            2561..2776
                     /locus_tag="ACHGMT_001768"
     CDS             2561..2776
                     /locus_tag="ACHGMT_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835506.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(2993..3249)
                     /locus_tag="ACHGMT_001769"
                     /pseudo
     CDS             complement(2993..3249)
                     /locus_tag="ACHGMT_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3436..4347)
                     /locus_tag="ACHGMT_001770"
     CDS             complement(3436..4347)
                     /locus_tag="ACHGMT_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFN6835507.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(4366..5370)
                     /locus_tag="ACHGMT_001771"
     CDS             complement(4366..5370)
                     /locus_tag="ACHGMT_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFN6835508.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(5367..7490)
                     /locus_tag="ACHGMT_001772"
     CDS             complement(5367..7490)
                     /locus_tag="ACHGMT_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFN6835509.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(7495..9942)
                     /locus_tag="ACHGMT_001773"
     CDS             complement(7495..9942)
                     /locus_tag="ACHGMT_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN6835510.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(9929..10318)
                     /locus_tag="ACHGMT_001774"
     CDS             complement(9929..10318)
                     /locus_tag="ACHGMT_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFN6835511.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(10378..10881)
                     /locus_tag="ACHGMT_001775"
     CDS             complement(10378..10881)
                     /locus_tag="ACHGMT_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFN6835512.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(10894..11118)
                     /locus_tag="ACHGMT_001776"
     CDS             complement(10894..11118)
                     /locus_tag="ACHGMT_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835513.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(11222..13132)
                     /gene="ltrA"
                     /locus_tag="ACHGMT_001777"
     CDS             complement(11222..13132)
                     /gene="ltrA"
                     /locus_tag="ACHGMT_001777"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFN6835514.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(13878..14015)
                     /locus_tag="ACHGMT_001778"
     CDS             complement(13878..14015)
                     /locus_tag="ACHGMT_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFN6835515.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(14012..15196)
                     /gene="mobT"
                     /locus_tag="ACHGMT_001779"
     CDS             complement(14012..15196)
                     /gene="mobT"
                     /locus_tag="ACHGMT_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFN6835516.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(15455..15745)
                     /locus_tag="ACHGMT_001780"
     CDS             complement(15455..15745)
                     /locus_tag="ACHGMT_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835517.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(15735..16877)
                     /gene="dcm"
                     /locus_tag="ACHGMT_001781"
                     /pseudo
     CDS             complement(15735..16877)
                     /gene="dcm"
                     /locus_tag="ACHGMT_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(16953..17117)
                     /locus_tag="ACHGMT_001782"
     CDS             complement(16953..17117)
                     /locus_tag="ACHGMT_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835518.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            complement(17110..>17201)
                     /locus_tag="ACHGMT_001783"
     CDS             complement(17110..>17201)
                     /locus_tag="ACHGMT_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFN6835519.1"
                     /translation="SEVHYATDNLELFLPLGYSRKDLEVQLHD"
BASE COUNT         4837 a         3687 c         2999 g         5678 t
ORIGIN      
        1 tatcatagca atgtatttga aaagcaaatt ggaaaacaaa taggtgcaac accataaata
       61 tgaaaaaatt gggaaaaagt agaatggaaa tttgcttacg tctcacgaga tagctcacac
      121 tattaatctg aagaacaatg aagtcgactt ttagaaattg ctagaagaaa gtaaagcgaa
      181 ttatcactag ttactataga gtaagttcaa ataaagcatc tccaacttac atggttacat
      241 gaggatatca aatcgaaatt actcaagctt cctatggcta gagtcgcgag aagttgttgg
      301 gcagtagaca taggttatat tgttcttagc tacacgatca ttagaaagga aatcctatta
      361 tgtatagtta tcaagaagat gcaaaagtcc aaaaaaatcg ctggtggatt ttgattgcta
      421 tatctatgtt taccttcatg tccacattgg atggcagtat tgtcaatatt gcactaccta
      481 ccatctcaga tgatatgggc ataccaatga atcaagcgga atgggtagtg tccgtttatc
      541 tgatgattgt ctgttcttgt ttgttgctgt ttggtaaaat tggcgatagc cgtgggaaaa
      601 tcaaagtatt tcggattggt acgctgatct ttgtcttggg atcgctgttg tgtgggttca
      661 atcattcgct aggtatttta ttgttcgcac gggtggtaca agccattggt gctagtatga
      721 ctatggctac aaatgccgga atcatcacgg aagtctttcc catacatgaa cgtggtcggg
      781 cattgggagc aataggtgcg tttgtttcat taggctcgat tgctggacca ggtcttggcg
      841 gcttgatttt gtcccaattt tcttgggcgt acattttttg gatcaatgta ccggtgggaa
      901 tcgtaacgat gttgattgct gaaaaattct taccaaaaga tatcaccatg agcggccata
      961 caatcgatgg agggggcttt gctgccattg cggctacgat tatcagcttt tttggagcaa
     1021 tctttttggg gcaagaaagt ggctttttcc atccagtccc ccttgtactg ttcgtaattt
     1081 cagtgattgc ctttgtcagc tttttaaaag ttgaaaagaa agcagcctct ccattgattt
     1141 ctttcggtat ttttaagaac aagattttta ccatgagctt gattacagcc ttgttgattt
     1201 tttcttccaa cttctttgtc aatgtagtga ttccttttta tctgcaaaat gctcgcgggt
     1261 tgagtcctag ctatgctggg ctgttgatga tggtctttcc tttgatgatg gtcgtcgggg
     1321 cgcctttaag cggctacctg accgaccggg ttggcccgga gattttggta ttagtaggct
     1381 tgggcttatt gtcggtaacc caggtcatgt atatgttttt gtcagtggac tctcctttgt
     1441 ggctatacat tgtcgctact gccatcatgg gtgccggcaa tgccttgttc cagtccccca
     1501 ataatacgat ggttatgagc tcggtcacta tcgaaaactt gggactggca ggctcgatga
     1561 attcctttgc ccgaaatttg gggatggtca gcgggatttc gctttctacg acggtcttat
     1621 atcaagcgat gtctgctaag atgggccagc aagtaactaa ctatatcgca aatcggccgg
     1681 atgtcttttt atatggaatg aagattacct ttttaggcag ctttgttttg tgttttgttg
     1741 cctttttcct gactatctgg cgtatcaaaa aacaaggagg tattcagaaa aatgtctgaa
     1801 aaaaccactg ttgaagaatt tttggaggcc tgcgtatggg tgagtgggat ttccatggat
     1861 gatggaagat gaagtgaact tctaaaagcc gaaaaactca agtattatct tcaaaatact
     1921 agtgctcaat gagatgcttg atttgatcac aagaagactg cgaaagccgc tggcaaccgc
     1981 aagctaggta tcccaccgat ggactacgtt ttagaggcta ctggttatga atacggagcc
     2041 aacgctctca tctgcataag aatacatcac ccagattatg taaagatttt cgatacttct
     2101 gtcaaaaaat cattaagata gataaatgat ttccgtctag tcaaattggt tcagaatgta
     2161 gacacacaga tggtaagaag tcactcacaa ttcgagaatg gacttgtcct atttgccgta
     2221 tacatcataa tcgaaacgtc aacgatagta tcaatatttt gaccaagggc tatgaataca
     2281 agcattgcct tagaaaacat caagaacctt aggaacaacg gggatagctt ggttaataag
     2341 agataccgct gtttaggctt gcaagtatac tcttttatca agaattccgt ggctttagtc
     2401 atgagaggct taatactatg aaaattttct ttagcatttt tgatttaggt tccttttcta
     2461 attataataa gatgaccact acagtaacct actgaaatat ataaacagcc actgtagtac
     2521 cattttcaaa gtagtctatg agaggagaat cttcattctt atgaacgctg gaattgcgga
     2581 tatgaattta ataaaaaaaa cccttaatga ttttacttcc aattcaatat caaaaggtac
     2641 tggtatcaat ttaagcacaa taaaaaagct gaaaagtggt gaaagatcag tggaaaagct
     2701 gaatctttta gatgctataa aaataacgga atttgctatg aaaaatggaa aagctgagat
     2761 tgaaatatgg agataattat cgtctagtga attacttgcc acttagctga atttaacgat
     2821 ccttggtgct tgaaggggga gattcttggg aatagagcat acttgcaagc ctatgtcttt
     2881 actaacaacg atatctctta ttaaccaagc tatccccgta gttctaaggc ttttactttt
     2941 atctaagcaa taatcgtatt caaaggaaat ttttgaccct tagaaagtct cattttatca
     3001 gacgtgagct taggcattgc tgaattttgt taattcggtt ttaggttgtg ctttgagcat
     3061 aatatgaaag tattctttat cacgatttca ttcatctaag gtaatgtgat gtgattctga
     3121 aattctttca aaggcagttt ttgcataagc tgacattaca tcatcaatga catgtttaca
     3181 atgtttcact actaaaataa gggggtaacc aagaagaaaa actgaatgat tatttgtatc
     3241 taattccatg gtatatcaac catttcctat atagactgat tatgacatgg attaaaataa
     3301 aaaggcacta tgtcttaatt gtcggttttc gagttaccga atggcattca tcacccacct
     3361 atagaggatg ggcgacttct gcctgaaata agttacacta cttataactt ctaataaaat
     3421 tagattgagt cattattatt taacatcaac gattttccag tttccatttt tttgtaatac
     3481 taatttgtac tgaaagtagc tagtggtttt tgatacatta tcaaggtatt ttatctttgt
     3541 actcactaga aaattttttc cgttttgtag ataaactgga ttctctaact ccgaaaactt
     3601 taagtcaata ttgattggtc gcatcgcacc attttcaaca taatactcca attctttttc
     3661 agaagctgat ggataaagtt taaaaaatgt ttctaaaaaa tcggtgattt cttccgttgt
     3721 tttggcatca atttctgaat cgttttctac ttgttttgct tcatattttg ccttgtctgg
     3781 ctttccaatc attgttggca aactagttac taaatgattc ctgttttcat cttcaaagat
     3841 tgtcactcga tatgcagaag aaattgattg cgtctttttt ccctctgtaa tatgttgctc
     3901 cactaaaaat gaaacgacaa attcttccga gtgtttttca acatctagaa tttttacaga
     3961 ttgaacttcg gaactcgtgg gaatatctgc tcgaaccata tcttggatta aagcgagacc
     4021 gttttctgta aggtactgtt cgagattact cattctcttt tcaattgctt ctttgttatt
     4081 tttccatgaa aaatattctt tcgcaaaatc ctctgtaaaa ctttcaacgc cagaagtatt
     4141 cactaccttc tcttttataa ccactttttc atgagttgta tgttgatcaa tggcggtgaa
     4201 atttttataa acgccaaaag caagactaca agacaataaa agccaaaggg caagcaccat
     4261 ttttcgatgc ttacccacgg atacaactcg ttcttttttc ttttttgggc tcttttcctt
     4321 ttgattccgt tcaattttta tcttcattct ttttcctctt ttctcttatt tgtatcggcc
     4381 agctccgata agatgctgcc gccaatagtt ttccgttaga ttcgtccacc ccactggatc
     4441 tcctgcatga aacatgcgat tctcccctgc gtaaatgccc acatgggtca cataagtacc
     4501 ggcatcatag gttgaatgaa agaaaactaa atcacctggt ttggcatctt ttagatcaat
     4561 atgagtcatg gcatcatatt gatcctgagc cacccgagga agtttcaggt caactttggc
     4621 ataacaccat tgggtcaagc cactacaatc aaaccccgtt tctggacttg agccaccaaa
     4681 cacataggcc gttccttcat acttaaatgc ctcatcaaaa atttcttgga ctattttgtc
     4741 atcaaactga gttgtagtta ggtattgctt cactaataaa cagtaaaaca tattgccata
     4801 attataccgc cacccaccat tttccttaat cgcgattggg ttggggtaat cgactttttg
     4861 accacctgat ttttcctttg aaaaagattc agctaaagca aaactatgct tcttcccgtt
     4921 tttcccgaca tgatctagat acccgccacc atagttatag gcctgaataa tactatcttg
     4981 atcacaccct agttctttgg aacgcttaac caaagacgca aaatagagac agccttgttt
     5041 gatggatgcc tcggtactta atgaattagg tggtaatccg gctgactcag aactttgcat
     5101 aacgtcttcc attgtgccac cagattccac ttgaataatc gccaaaagaa ttggaatata
     5161 ctctgttata tccgattctt tggcatactt ttccaccatt gcacggtgtt tcaatacttc
     5221 ttctgagaca ctagttccgc caggagaagt gccagaacta ccatttccct cactgtcttc
     5281 accaaacaac aaacccacac acatcaaaat acccataaaa gagatggtca gaaaagttaa
     5341 tgaaataaca cccactttct tcaagttcat tttttctcac ctgaccttga ttttggacga
     5401 tggttcattc ttttttgtac tttttgattg gtctgctttg gcagagtgga cctctgtttc
     5461 acttgttgga aagaattttt cttacttgct gattggcttt tcccttttga aatgatttct
     5521 tgactagtta gttttcgttt gatttcggga ttcagatttg ctctcacaat ccgctttggt
     5581 gcttcttttt tcagcatcat atctttctga ggttgagctg ctttatctat tggaatattc
     5641 gcccttcttt ttccctgacg atttgttgct tcactcagta tcttgcgttt ttcgtccatt
     5701 ttcgcatttc ttcgttgtcg ttgtttttct cgcaaatcca ctcgatccgc tttttcttgg
     5761 aacagtccgc gcttgaattc ttctggtgct tttttcgact tttcgattcc tttatggaga
     5821 ttgtatttca aattcgttgg cgtattcctt acctgatcct ttacttgttt agtcttatct
     5881 ttcaccaaag cttgggtatc caaaactttg ccgatttttc tcccagccaa attcattcgg
     5941 gattgtttct tattatttga aactggattc tccttagtta agtcagatag tatttcgtga
     6001 tcgttcggta atcgtgaatt ctttcgtaga gaagaaccgg atggcttaaa tttggatttt
     6061 gattgttcct ttgccaactt atttccgact gctgcaccac caccaaaagc caaagctcgt
     6121 cccacatttc gattgagttg ccgcaatttt cgattcatta acatttgcgg ttttcgcatg
     6181 acgcgtcttc ccattgactg gctatcatta ctctggaggt tgaacatact cataatgtcg
     6241 cccaatttga aatagatacc ggcaaaggtc acaatttgca gaaaagcaac catgaaaaat
     6301 ggataggttg ccgaaatatt gaaaaacatc gtggagatgc taaatgcagt cgtaatcacc
     6361 aaagtaacac ccgctcgcat cataatcgta ttgaataaac gaataatcgc cttctttcct
     6421 aaactttcat aagtcggcaa catggacaat aagaaactaa ttggcagaaa catcgcaaag
     6481 ataatgaaga gaatttgcga gaacaacata atccctgtta gaagaaaaac aaagaaggaa
     6541 atgccaatat tgaacaatac caaaaagacc acggtcccta aacggttcat ggtcttggta
     6601 atactgagat tcgcattgtc attgtcttca atctctgcct tgaccgcttc ttcccgatct
     6661 ttgcctttgt tttcatctgg actcacagat agaatcttgt tgacccggtc ttcaccaatt
     6721 tcttctatgt tggaatcatc aaattgcagt agtaaccaag gttgttggac ttgaatcgaa
     6781 aacaaactat cccgaatcaa atccacacta tcttttcctt ggctttctga attgggtaca
     6841 acgatctcgg ttccaagggt aagggccgct tcactaacat ctgagctaaa gtcattgatt
     6901 tttgtgatat aggtaggcgc ataagcaatg aacgaacccg atagtagaaa aatcatcaca
     6961 aaattaatca cggctcgaac tgctttcgtg gtttctcgtt ttaataatcc ggtatacgcc
     7021 acatacaccc ccagagctaa aatcatcaat aatagaaacc caaaataaaa tccggaggtc
     7081 tggagtccat tttcagtaat accagccaat gtttggatat tctttccaat actttcagcc
     7141 gtatccgaaa tgaaatctaa tttgtaagcc tcttgaacca cataacccgt ggcgttcgac
     7201 aaatacaaac tcaccgtcca aatgaaatta gtaatggcat aaagaccata catcacgctt
     7261 ttccctaagc catcacccca attccaaggc aaccaatccc aagagctatc cacaaagaaa
     7321 tctagttgat agttgttcag tgaatatttg gaatatagat ttcctgcttc aactgtatca
     7381 tcaactagcc ctgtcgcctc ggctaccgta ccaactaaac tcaatagaac tatcagagtg
     7441 agtatgctaa taaaaacgat tcccacaatc cgaaaaagtt tctttttcat cccctcactc
     7501 cattctctct tgaacaggtg gacgggtatc aaaggcatgg aagagttctt caaagactgg
     7561 atgtacctga accacgccta cccgaccata caaatcctgc atcaagcatt ggccattttc
     7621 caaatctcgt aggcgttttt gattgctttc atcttctcga tccaagccaa agaattccaa
     7681 ggttttcttg atttcatgaa tatcggtact gcgaaacgca aacttcaacc cgatattatt
     7741 cttcaagcgt tcatccagta aatcatcact attttgcgtg acaaagtaaa ccccggcatt
     7801 catagcccgc ccagcacgaa cgagcttatt cgataatgtt ttcccttgcg ctacttgaag
     7861 aaaactccac gcctcatcta agtctacaat tttgaagatt tctcgattac tatggataaa
     7921 gtccaaggca aaagtggaaa tgacgattag catggaaaca ctaagcaatt ccatggtggt
     7981 atattcttca aaagtggttt ctgcatctgg taaaaccaaa tccgctactt gaatgatatt
     8041 taactgccta tctaaactaa tcgactgggt aatcgaacca tctgagaaaa gcaaatgggc
     8101 aaaatcataa tcggtaaagg actcgatgtg atccgcgata ttttccgcaa ctggtgagcc
     8161 ttcttttcga agctcattaa ttacttgtaa taatccccgt tgattgcttt gggtaaccgt
     8221 ccgaatggcc ttacgcaaca ctggaaactt ctccccatct cgactagaaa ttcctgttaa
     8281 aaaggtcaga atatcaattg ctagactttc agaatccttc tttttctcca taatcacata
     8341 aggatcaagt aatccttgat tttttggctt actagttaag ttgagaatat tgatttcatc
     8401 cgcaatttct ggcaaggttt ctttccagcc accccgttcc ccttttggat cgacaatcac
     8461 agcttgtcca ccaaaaagga cggcgtaata aactaagaga ttgttactaa aggactttcc
     8521 gccaccgagt gaaccgagga atgccgctgc tagcgcattg gtaactgaat ccttgacccc
     8581 ttgtgaggcc aatgccggtt tcaggtaaac attgcgcccc gtatctacgt tataaccaaa
     8641 gtaaatgcct tccaactccc ctaacatctg agtggcgcca aaaccaagtc cggcgagaaa
     8701 atcagacgta acatactgga tataatcatt catgtatcgc ttactagaag gtaagaattc
     8761 ttcatgtagc cccatcatat cgccaaaagg ccggactagt tttacattgg tatcatcata
     8821 aaaatctagc acttcatcac accggcgctt tagttcatct actgattcgg cacttactcg
     8881 aaccacatag ctcagtttgt acatggattc tttggactga tccaaggtag tttccaattc
     8941 atctacggaa tccaatgcat ctaagacatt ggaatttgtt tcattgtcag attgataggc
     9001 atggttgtcc aagtctttta attccttttt cttattgcga acggtagcaa gtgccttttt
     9061 gttcgtcaca atctccacat tcatactagt gtcaattggg aatgtaaatt gttgttgctg
     9121 gtaatagaaa agttctgagg agggaaactc catttccccc acaattgtat taattgttaa
     9181 gtacgctaca tatgattcat gattctcatg ttccatgcat aagtaccggg gcttttcttc
     9241 aatcaaacaa cgactgggac gtaataagtc gtaacgtttc accaaagttt ccgacttcag
     9301 ctttttcttt ggtagctgaa attcatattc ttcaaatggt gtaccctttt cgccatagat
     9361 atgttcaatc aaatacatca gatcactcgg tgtgactcgt ctgactttaa atcttcgagc
     9421 caatttactt tccattagtt tttcaagctt ggtatatcgg cgaatttctt cattggataa
     9481 ggaaacaaaa tcgcccatca aatgatgatt cactccatag acaaattctt gaaagccgga
     9541 gaagaaagat ttcttgagat tctttagatt gacctcttca tctgtcgcaa tcagtttgaa
     9601 gccaataaaa aagcgataat caatctgact atcgccaatc atggatacta atgcctctgt
     9661 ctgtaaatca attcgttgtt tcgctacttc tgttaatcgc ccggtaattt ccttttttga
     9721 tcgttcttgc gtggcacgaa tactactttc tgtggcaatt tgtaaggcat gaatcttgcc
     9781 ttcacgattt tgcgcaatca attgacgaaa gttatcgtgg acttgatatt tctgttcagg
     9841 tgacaaaaag gagtaattgt aaggtattaa ctcatagtag gcaaaacatt ctccctcttg
     9901 attaaagaca agattatttt caatatactt aatcgggtac atagttcaca ctccttacca
     9961 gagtaatggg ttcgtcccat cgtttcttct gttctttgac tttctttcct gcgaaagtga
    10021 ccttaaatcg taagtgatag ctaataaaag atcgtaaaaa tcccaatggt ttcttaccgt
    10081 caaacgtttt ttgcgacatg aaccaagtaa ctgccaccgg tatacctaag tattttaaaa
    10141 aggccccctc tatcatagat agtggaggca catgggcaaa tacgatgaca aacagcaagg
    10201 ataaaacaaa ccaagccatc tggttaaaag tcacaggaaa aggcagttga aagtcattaa
    10261 ttgcataaat gaccttttca actgcccaaa tactggtgta gctttttatt ttcttcatca
    10321 aatcattcct ttaaaaagaa ggatagttta gaaatcaact acccttcaat aattttgtta
    10381 atatggatac tcaaaaacac ctcgactcgt taccaaataa ttgcctccaa tctccaagtc
    10441 tctaccacac gcatcaaaat caatatacgt cttcaagtgt tctggtactt ctccaaaatc
    10501 accagcttct acaatcaaga aatatgctac atcactcata tcatcgcaat ctggataata
    10561 gataatatca tccacatgct ctaccatctc ctcaaaagaa ttgaagaaag catgttgaat
    10621 ctcagaagca acttcaccaa ctggcgttcc ttcaagttcc tccgctaagc cacacaaccg
    10681 attaatttcc tcaattggcg tatactcatc aatctcaaag ggaagttcat aatcatgaat
    10741 tgcatattcc tcgtattcgt catttaaccc aattcgttct ttcacttcat caaaatcgac
    10801 aggtggggta aaccacgccc ccaccaattc accttcgtta tatttcccga ggttcgcaat
    10861 ataaacacgc atctgttcca tcgtgcttcc cccttacgca ccgacaattc ggttaaacaa
    10921 actgagtaag acatccttca ccccagaagc attaaagacc aatccgaccg caatcaaagc
    10981 aatcaccaaa aagccaatca gtttggaaaa ctcccgctta aatcctaaaa acaaaccaat
    11041 gaccacaatt gccagtaaga ctaaggattg ggcattggtt aaaaaccatt gatatatgga
    11101 taggtaagtt cctgatatta taaatatctc aaacttttcc ccccattcac acggagcgtg
    11161 cgactttcac cgcactccgc gttccatcgt gtttatattg aaaagtattt atgtagtaga
    11221 cctatatcaa gtttaattct tgattgccag ctaatactct atatttattt agtcttatga
    11281 gctggacttt atttagcttc aatagagcta tatatttttg tattgtttct tctgttttag
    11341 cgtgtattag acggtgtacc gcctctttta ccaaaataag atttttataa tcgtcatttc
    11401 caccagctcc agttggtaca atatgatgac aatgtatttc attagcttgt tgaaattctt
    11461 ccagagttat agcacatttt ccatgttgag cagaaaatag cgaaattcga ttgtctgcgt
    11521 attctattgt gcgattttcc aaagataatt tcattaactg gtacatcagt aatttatcaa
    11581 cacttagatt ggtgtggata aattttcttc cctctagagt gtagcaattc gctaaataca
    11641 cctttgccat tggttttctg tgttggatat atccaacagg gaaaataggt tcgccctcgc
    11701 cagcaacata ccgaagcatt ttagatttac catatcttgt tgcctccttt gtcgtaagtt
    11761 gtctgcctgt ttttctcaat ctgccccttt tgtttgtacc taatctgtta gtgaagatag
    11821 ccattacagc tctattcagt ttcatacaat ccagatttac attcgttgca atctgataat
    11881 aattttgcat acctaatacc attgaattat aaagtcttgt ttcttctaat tccgttttcc
    11941 cttttgaggg agaagcaatt ttctttgcct gtgataccag cttgtctttt tggtttttga
    12001 attgtttatc acttatatgt gatttaacaa ctaacttctt tcttctcggt atcattttaa
    12061 tcttgaaacc aagaaagtcg gagtatcttt ttctaacatt tacaattctt gttttttctt
    12121 gtgatacttc caattttaat cgttgttcaa tccattgagt gattgctatt ttggcttttt
    12181 cagcactttc cttgtagcgg cagaaaactc taaaatcatc tgcataacga accatataca
    12241 tttcctttaa ctttgttttc ttcatttctt tgtaagcgtt gcttttacat gggctaccgc
    12301 ttttattgat atgaaccaca tagttttttg taacaggatt ttcctgccat tggctttcta
    12361 cccaatggtc taactcatta agaacaatgt ttgctagtag tggggatatt attccccctt
    12421 gcggagttcc tttctctgga tatgttattg taccgttagt cattttgatt ggagctttta
    12481 atatcctttt taaaatgaat atcaagtgct tgtcccgtat tcccatagcc catatctgtt
    12541 ttatcagctt ggaatggttc acgttgtcaa agaaaccctt aatatcaaat tcaatcacat
    12601 aatgtagctg gctgatttgt aggcggttat aggttgcttt tattgcattt tcaactgacc
    12661 tattaggtct aaaaccatag ctgttttcac taaatttagc ttcgcagatt ggttctaata
    12721 cttgttttat acattgttgt ataagcctgt cccacataca aggaatacct agaggtctgg
    12781 ttttaccatt tggttttggt atttcttttc gcctcactgg cttaggtcga tacccatgtg
    12841 gactgcctgc aacaatgtat cttacttttt tgacaactgt ttctgctggc aatttgccaa
    12901 tatctttaat tattgtaccg tctgttcctg ctgttttact gcctgcattt gctttgatat
    12961 ttcgatatgc cagtaatata ttttctctcg ataggataat atccataaga tgagtgaaaa
    13021 tatctccatt cttacttttg gcatataaat catcaaaggt gttttgcatt tcgtaatatt
    13081 cagcgtgtct taaatcttct acacacaatg tttcattttt atttttaggc ataaggcttc
    13141 actcctttac ttgaaattga gtgagctttt ttatcttact cgtaatcctt attgaaacaa
    13201 cataatactt ttctaatata tacacgactt gagggcattc ctccattctc attacaagaa
    13261 tttcttaggt tcgccctctg cttttcagca ttagttcaac tgcttattgt tcttcgtcag
    13321 tttattctct tgatgagaac ccaatacctt tcctcgttcc gataatctta tctatacata
    13381 tatatcctta ggtcactagc tataagcctg ctggcttgat accgcctgta acgttatatg
    13441 gcatttcata acctatattc ttactttgcc acaccagcta cgatttctcg tacctttgca
    13501 tttctacaaa gttcaaattt agacccgtac attcgctgtt tcgtcagttc cttatagaac
    13561 attctaacca tagatatttt atagaccccc gacctgttgg atacgctacc cacctctgga
    13621 tagttttcga tagaagatat atgttcatca actattacgg tatctttcag ccgtctttac
    13681 cgagcttcaa acattctgag tttcctcata caatgcttgt cggaggctca gggcaggcgt
    13741 ttcagagcgt tacttcatca ttccacctgt cagattatca gttctcctaa taaccaaact
    13801 ttttcgttct tttattagtg ccttagcttt tcactaagga acgagtcgca cataaatttt
    13861 gtccgaaatt catcttgtca ctttcctttc ttttccattg ctcgatccgc tgctgcactt
    13921 gcattcatca agcacatcac agtaacacca agcatactgc caccaaatac caaaaaaata
    13981 tcttttacaa ttcccatgac gattcctctt ttcatataat caaattttca attgccatac
    14041 tttgttgctc caaaatcttt ttatgtcggt ctgataagcg tgcattctgt accatatcct
    14101 ggatataggt tgtccgattc aatcgatcca actccttagc cattttccac gtaggggcga
    14161 cttgtcggcc tagccaattc aaggttcgtt caatggtata cggttttggc tcggaagtta
    14221 attgaatctc tttccgattc cgtccaataa aacgccccca ttgttcattc aagggccagt
    14281 tggttcgcct tttaccttct actttatccg caaatcgcaa gtaacgattg ataatggaaa
    14341 acgtggtgct ctcaatgcta cgacctttta acaaatcttg aatcgcatga aaagcacggt
    14401 catttttcag tcgaatctca aaacgatttt tcgtctcagt ttggtctaat ggaatcccta
    14461 acttgatata ttgttcatag tctttttcat acaaacagaa ataaacctca ctcttgagac
    14521 tgccgatata taaggtattg cccatgccgt ccttttcatc agcctttaaa agttccccgg
    14581 aacgataact tttaaaggta cgaaacaagg agatacattc ttccttctga cattttttcg
    14641 ttagctcagg tatatccaat agccccactc gatcattgat tgccaagtcc aagcgtttca
    14701 tcacgccacc ggcctttaga caatcctcga aaaagtcgta ccagctacgc ttttgagcga
    14761 gtaaaaaagt ttcaaattgt cggcaacctc gcccctttaa ttctaaaagc acacctttat
    14821 cttcttcatg agacaacatc accacaatat cacccatgac atactgttct tgataggagt
    14881 aaaaggcata atcttcatgg agcatatact caaatttcaa atggagaatt tcttgaataa
    14941 tttttcgtgc atccgttgtg ggaaagcgaa tccttacata gtcaaacagt aacgtcaaag
    15001 gcaaatcggg attaaagctt tccagttggt caaaaatttt cctcatggtt tttatagatg
    15061 cggttacgcc accattttct atctgattta agtacggccg actaagtcca caagccgtag
    15121 ccaatttatt ttgtgaaacg ccgtattcgt tgcgtcggtc ttttaatagc cgataatcta
    15181 aattccgttg tgccaagttc tcacctccaa attcaaggtg ttaacttttt caaatgagtt
    15241 aacacctata caaattactt actatctttg atacaacaag cattcttaat ggttgcttac
    15301 ttattttttg tgtttttgta cccccctgtt agatacgggg gtgagacatc atggtcggct
    15361 aacgccgtcc atgaacaggc acagcaccac ggaggatttc tgcttcgcga atcctccgcg
    15421 gtcagcgcct aacagtcagt tgtgttggtt taggttactc atctgtaata ccatttactt
    15481 ccaactctgc tttcatttct aacaagtatt gacgaatatc tggttctttt gcttggtccg
    15541 caatccgctc aaattctgca attacttgat ttatttcaat tcgagcatcc gtctcctcat
    15601 tcattaaata tggatcgcca gcgactttga caccattctt gcgaatatca aattttaacc
    15661 gtcgcaattg ttcttctaat tctgggtctg actgaatctt tccaatcacc caataaattt
    15721 ctccaatcaa ctcatcataa accatgtaca tccaactctt ttaatcttct ggcaatatca
    15781 aaaatgaccg gaactgttac agagtttccg gcttgcttat acaactgact atctgaattg
    15841 acttgagaat ctcgatcaaa agcccaatcg ggaaagccct gaagtcgcca acactctcta
    15901 ggtgttagct tccgaatttg atactgatcc gtgacgatcg cttgttggtc tcctgttagt
    15961 aaggtatgaa cactctgttt tcccagacga cctcggcgac ttgtggaatt aggaaaagcc
    16021 agattcacac tgtcttctgg cttcacctct tgatattctg tttcattttt tcgaatcatg
    16081 atttttgtac caccttgttt cgttgacaag gtgggggcgg ttccagtaac ttcataaact
    16141 cgattacctt gttcaaactt tcccggtaat agtcctgcta cagagattcc atcccttgct
    16201 ggcagtgcga ttttgattcc ttcatcacct gttaaggttg gcgctacgcc atctgaacca
    16261 tagacttgtc cacccattcc tcgattggaa ggattgatat ttaccaaatt tctgatgttt
    16321 tgatcagatg ttgaagacgg tccaatgata ggaaatactt gttcggtaca tgcgtctcga
    16381 gaatgtccga taaggaatat ccgctcccgg ctttggggaa tgtaggcctt tgagttaagc
    16441 acctaccact ccacgtcata ccccaatcca tccagcgttc ggaggatcgt ctcgaacgta
    16501 gcccctcctt cgtgattgag caatcctcgg acgttctcaa ggaataaaag cttaggtcgg
    16561 agaagagtgg tgaaccgagc gatttcaaag aataaagtac ctcgagtatc ggaaaatctt
    16621 tgccgctttc ccgcaattga aaaagcttgg cacggaaatc cgccgcaaag gatgtccact
    16681 ggtccgaggg attgaataaa ctcatctgat acacttgtga tgtcatgcat ttccacctcc
    16741 ttacttgtat catgaattgc tttataacta cgtctagcaa atttatcaat ttcacaaaaa
    16801 ccgatacaat gatggctcgc ctgttccata cctaaacgaa agccaccaat accggcgaat
    16861 aaatctaaaa atctcataca ttacctcatt cattgcaaag aaaaaagcca agaacatttt
    16921 gctcttggct catacattat taatttgtcc tactattttt tatttaatcc aactaatttc
    16981 cgttgttttt cattggcctg atcggctaca tatttaatca ccgttttgaa ctcttttgca
    17041 gtagcatttt ccgataaatc cgcaatgctc ttattgtatt tctttacaat tttcgttact
    17101 tgttctttct cagtcatgta attgaacctc caaatctttc ctactatacc ctagtggtaa
    17161 aaataattct agattatccg tagcataatg aacttcggaa a
//
LOCUS       DAZBSA010000059        16717 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000059 DAZBSA010000000
VERSION     DAZBSA010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.059"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(254..1465)
                     /locus_tag="ACHGMT_001784"
     CDS             complement(254..1465)
                     /locus_tag="ACHGMT_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6835520.1"
                     /translation="MSEVTGYLEQVDNETYRLRAVLGYKPDGSAKRKSKTIKASSKRA
                     AYKELNIWLEQFEGMTDDSLDLFNITFGEFYRKIWLSEAEKNLEPKSYHNYKRMIENR
                     FLDKFDFIPLIDIKPYMIKKIVVNAQRINTKDPGRNSDKPLSRNTKLRMLYAVNNLFL
                     MAKNEYGAIKENPVENVKIPKEKGVKKNIEEPYSEEEIHAMLKAAFEESIEIKTLIVL
                     AFITGARQGEIAALEEKDIDFDKQEIRFHQRISEVDGKSDIRLLPGLKNDDDEKIVTG
                     PAYLFDMLDELIKENKKIRWKLNIKKLKHYFIFDTKQDGTLPRGSYLYKKFKRFTKRH
                     NLRHIRFHDIRHTSATYLLSDPNMTPKELQKRLGHRDFNTTMNVYGHVLRKEKDTATT
                     AFENLLKKDIK"
     gene            complement(1584..2219)
                     /locus_tag="ACHGMT_001785"
     CDS             complement(1584..2219)
                     /locus_tag="ACHGMT_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFN6835521.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWVLIVLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTTKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAKNNVLQAQTGYFGTETVSIALHN"
     gene            complement(2355..3008)
                     /locus_tag="ACHGMT_001786"
     CDS             complement(2355..3008)
                     /locus_tag="ACHGMT_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835522.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQVTAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFFKYGIQ"
     gene            complement(3111..3317)
                     /locus_tag="ACHGMT_001787"
     CDS             complement(3111..3317)
                     /locus_tag="ACHGMT_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFN6835523.1"
                     /translation="MPSYVVLQVVLKEKFIGKGSQNLSELENTINRQCSKGYRLHTIS
                     TTNGGSKGFGGGDRIQATLVFESL"
     gene            complement(3393..4139)
                     /locus_tag="ACHGMT_001788"
     CDS             complement(3393..4139)
                     /locus_tag="ACHGMT_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004564412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFN6835524.1"
                     /translation="MKQTTQQRLNQLMSERNLKQVDILNMSLPLQKETGIKMSKSHLS
                     QYVNGKSSPDQHKLYLLAKTLNVSEAWLLGYDVPKEDKKNNIPSIETIYNQLEKPRQT
                     KVYNFAEQQLEEQNRGNVVHFPKKEKLPTIKNSASAANPTELVYGDTVVEEEEFERVP
                     NNADFAVPIIGDSMEPVIRNGQFVFVKEQPDVEDGEIAIVEIDGDGVTCKEVYKDYES
                     QTIILRSINELYEDRVVSPEQIRIIGKVVF"
     gene            4299..4523
                     /locus_tag="ACHGMT_001789"
     CDS             4299..4523
                     /locus_tag="ACHGMT_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF739 family protein"
                     /protein_id="HFN6835525.1"
                     /translation="MSFDYSKLSGRIVEKYGTQYNFSIALGLSERSLSLKLNNRVPWK
                     STELQKAIELLDIPKEEIGEYFFNLEVQNV"
     gene            4547..5308
                     /locus_tag="ACHGMT_001790"
     CDS             4547..5308
                     /locus_tag="ACHGMT_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HFN6835526.1"
                     /translation="MNTQKFENNLFELEVKTENGESLFNAETVARSLGFVETKNGKEY
                     VRWRTVNGYLKKYLSQEVAKNDFISEPMVYKLAFKANNVLAEKFQDWLATEVLPQIRK
                     HGMYATDELLNNPDLLIEVATKLKEERTLRLIAEQRVNELQPKADYYDRILNNKGLVT
                     VSTIAKNYGMSAVSFNKLLHELGIQFNQSGTWLLYSKFQDKGYTHIEPFDYEDKNGNR
                     QVKTRMKWTQKGHIFLYETLKKNNYLPMIEREQTA"
     gene            5320..5577
                     /locus_tag="ACHGMT_001791"
     CDS             5320..5577
                     /locus_tag="ACHGMT_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835527.1"
                     /translation="MNEEMSQTIKVQKMIDDLTHAIDSQADKIIKELQGQKVWDAKML
                     FKTIKFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            5669..5920
                     /locus_tag="ACHGMT_001792"
     CDS             5669..5920
                     /locus_tag="ACHGMT_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HFN6835528.1"
                     /translation="MEQLALVNLSDLKASLAESEIANEVWDTKQAAEYLKTTTRTLTK
                     DAESGKIPAAKVGREWRFSSIALYQYLKGGKTWVNSTEH"
     gene            5896..6069
                     /locus_tag="ACHGMT_001793"
     CDS             5896..6069
                     /locus_tag="ACHGMT_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835529.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEYRAKKPTEGAD"
     gene            complement(6183..6590)
                     /locus_tag="ACHGMT_001794"
     CDS             complement(6183..6590)
                     /locus_tag="ACHGMT_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835530.1"
                     /translation="MSFDNTITISIILALVALISPSITAVINNKHVESMKDKEIELQK
                     HDSKTQTIQTTFSTFLNNVGICIGSNTDKNISAVKASGYAVLPYIQNEDIEVMKIFLS
                     RFGYGNTDAEQKSLETYLIDKVLPILNKSLEKL"
     gene            6751..6975
                     /locus_tag="ACHGMT_001795"
     CDS             6751..6975
                     /locus_tag="ACHGMT_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835531.1"
                     /translation="MISVKGLGDEIFEAMMSKAQQDIKEKIISAAAQGQTSCTVRSRG
                     TTPSFLLALEDEGILNIKRRDGSVRLFWQF"
     gene            6985..7179
                     /locus_tag="ACHGMT_001796"
     CDS             6985..7179
                     /locus_tag="ACHGMT_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835532.1"
                     /translation="MKDFDSLGARQQPPNEASPVGVDWQENPLYPGDTCYLTEEGYVP
                     VDAILEYVQQHYPKIELGGI"
     gene            7182..8204
                     /locus_tag="ACHGMT_001797"
     CDS             7182..8204
                     /locus_tag="ACHGMT_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFN6835533.1"
                     /translation="MANDLTQTTQRSLDEQVISNLGRLQEQGLEMPPGYSPQNALKSA
                     FFELTNNTGGNLLQMAANNQEMKTSISNALLDMVIQGLSPAKKQCYFIKYGNKVQLMR
                     SYFGTMAVLDRVTGGADITPVVVRQGDEFEVAMDGPNMVVKKHETKFENLDNEIIAAY
                     VVIKLTNGKETTTVMTKKQIDQSWAKSKMKDSGPQKEFPEEMAKRTVINRAAKTLINT
                     SNDNDLLVQAAKDTLENEFDNDRKDITPQTEKVATLEQKFFSNKKITEPIQKEPDPIV
                     IPDDIQNEVTRVADVPAHPEIEQAHLIENEDINPIQEELIDIPDFGREEGVEDVSEFE
                     DDEYPF"
     gene            8167..8982
                     /locus_tag="ACHGMT_001798"
     CDS             8167..8982
                     /locus_tag="ACHGMT_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HFN6835534.1"
                     /translation="MSQNLKTTSTLSDENYYSNEADWQYMSTSQYKSFLKCEAAALAK
                     LKGDWAPTSDPKALLVGNYVHSYFESKEVHEAFKEENKSKMFSSRKPYGLLKDFQIAE
                     QMIERLKQEEAFMNIYQGEKETIVTGELFGTTWKGKIDCLNVEDEYFVDIKTTKDMHE
                     RKWNENYGSRETFIVNFGYVLQMAIYQELLLQQYGKMFMPIIAAVSKQTPSEAKLITI
                     DQDKMSYELIMLKENIERIVKVKNGEERPIPCGTCEYCRGNLSITGFTSMDDL"
     gene            9000..9782
                     /locus_tag="ACHGMT_001799"
     CDS             9000..9782
                     /locus_tag="ACHGMT_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835535.1"
                     /translation="MNLGYIKLYRKVTSSFVWTNSDMFKLWILCLMKASHEDRKFLFN
                     GQEVRLTSGQFVTGAHAIAKEYNEGVPSDKAIAWRTLWRWLKKFENEELLTIQSNARY
                     SVITIINWSDYQSSDKPVTSDGQSSDKPVTTNKNDKNDKNDKNNNPRNSRKTREYADD
                     DPNKKLAILLLKLIRKNRSLKEPDLNKWANTIRLTIEADKRTGKEVQDMIVWATNHDF
                     WSTVISSPTSLRKNFDTMVGQKNKRKQQNISNDELPETGEDW"
     gene            9782..10630
                     /locus_tag="ACHGMT_001800"
     CDS             9782..10630
                     /locus_tag="ACHGMT_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN6835536.1"
                     /translation="MNKKLSAMAAPYGGLKTADHNCPKCGDPLYIWKTKNKDGTDRCG
                     PTCINKICGYREMVTKNQKEAIQKANEAMKKDAINRMLNSSMITDDAIWTFDFDGYKV
                     VDQETAQIKAMAQEWAKKIVNGSTIHAVITGRTGAGKTHLGAAVIKEVMRASNYKIAC
                     SFISYRELLEQLKFAMNDPEARKAVTGSLMAEIKKSDFVVIDDLGAELGRMEENNQAT
                     PYDVDVLTSLTEARLNKATIFTTNLSSKQLKHAYGERVFSRVMNGTKGNIAVFKTTTD
                     KRRNPV"
     gene            10627..10869
                     /locus_tag="ACHGMT_001801"
     CDS             10627..10869
                     /locus_tag="ACHGMT_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835537.1"
                     /translation="MTFVVKKMCYLDSRGRGEASVEFAKHHTTKEEADLVASVCGGEV
                     VEVIKPKRRFSKPKTKPVKKECCCPKSNQAWMRGAK"
     gene            11012..11380
                     /locus_tag="ACHGMT_001802"
     CDS             11012..11380
                     /locus_tag="ACHGMT_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFN6835538.1"
                     /translation="MATRSKYGNKKHEVDGITFDSITEAEYYDLALWQAQANGWKVKL
                     QERFELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQ
                     YQVPLILAKKYRNTFKEERF"
     gene            11395..11556
                     /locus_tag="ACHGMT_001803"
     CDS             11395..11556
                     /locus_tag="ACHGMT_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFN6835539.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            11553..11879
                     /locus_tag="ACHGMT_001804"
     CDS             11553..11879
                     /locus_tag="ACHGMT_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835540.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            11876..12691
                     /locus_tag="ACHGMT_001805"
     CDS             11876..12691
                     /locus_tag="ACHGMT_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFN6835541.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            12693..12818
                     /locus_tag="ACHGMT_001806"
     CDS             12693..12818
                     /locus_tag="ACHGMT_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835542.1"
                     /translation="MGFFMAKILLFLGLVGAAYLVYALLSQTDDKEDDNNDEMKF"
     gene            12830..13141
                     /locus_tag="ACHGMT_001807"
     CDS             12830..13141
                     /locus_tag="ACHGMT_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFN6835543.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEGS
                     K"
     gene            13138..13383
                     /locus_tag="ACHGMT_001808"
     CDS             13138..13383
                     /locus_tag="ACHGMT_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835544.1"
                     /translation="MIKKLIQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            13380..13796
                     /locus_tag="ACHGMT_001809"
     CDS             13380..13796
                     /locus_tag="ACHGMT_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFN6835545.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIYENSELLEVE"
     gene            13793..14131
                     /locus_tag="ACHGMT_001810"
     CDS             13793..14131
                     /locus_tag="ACHGMT_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835546.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMYGNSESAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            14128..14358
                     /locus_tag="ACHGMT_001811"
     CDS             14128..14358
                     /locus_tag="ACHGMT_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835547.1"
                     /translation="MKYEIPLSEADVQSIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSINDTFRLGS"
     gene            14365..14568
                     /locus_tag="ACHGMT_001812"
     CDS             14365..14568
                     /locus_tag="ACHGMT_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFN6835548.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASDAIRKFGISILSDDRKWEEKE"
     gene            14565..14861
                     /locus_tag="ACHGMT_001813"
     CDS             14565..14861
                     /locus_tag="ACHGMT_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835549.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKWTAKSKENLAVVRLVDMELINDK"
     gene            14938..15351
                     /locus_tag="ACHGMT_001814"
     CDS             14938..15351
                     /locus_tag="ACHGMT_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFN6835550.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDALIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            15364..15750
                     /locus_tag="ACHGMT_001815"
     CDS             15364..15750
                     /locus_tag="ACHGMT_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835551.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            16301..16528
                     /locus_tag="ACHGMT_001816"
     CDS             16301..16528
                     /locus_tag="ACHGMT_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835552.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMLREAT
                     PKEIDQYKLVLIGIGWFKDEHIQTNMKRWLR"
     gene            16525..>16717
                     /locus_tag="ACHGMT_001817"
     CDS             16525..>16717
                     /locus_tag="ACHGMT_001817"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835553.1"
                     /translation="MKLYYVEKANCWVVEGCLSKYATDIISTQFSELGLKVIFCGEFV
                     SVKEAVLPMTAKSRRSRGSS"
BASE COUNT         5667 a         2726 c         3485 g         4839 t
ORIGIN      
        1 aaataaaaaa agaacagcgt aaatgttttc gaaatgcttt cgcaccaaca tttacctgtt
       61 ccgtgggttt ggcacccaat gggccgtgag gggctcgaac ccgcgacccg ctgattaaga
      121 gttcgttatt taatggtatt ttaaatgaag ataagcgaat ttacagcatt tttaaatgct
      181 tatataccgc aaattatccc aagtttttgt atatcctacc cgagtttata cccgaaaata
      241 taatttttta gtgttatttt atatcttttt ttaataggtt ttcaaacgct gtagttgctg
      301 tatctttttc tttgcgtaag acatggccat aaacattcat tgtagtatta aaatctcggt
      361 gtcccaatcg tttttgaagc tcttttggag tcatattagg atcgctaagc aaataggtag
      421 ctgatgtatg tctaatgtcg tgaaaacgaa tgtgacgcaa attgtgacgt ttggtgaatc
      481 gtttgaactt cttatacaga taacttccac gtggcaaagt tccatcttgt tttgtatcaa
      541 atataaaata gtgttttaat ttctttatat ttaatttcca acgaatcttt ttattctctt
      601 tgattaattc atctaacata tcaaatagat aagcaggacc ggttactatt ttttcatcat
      661 catcattttt caaacctggc agcaaccgaa tatctgattt tccatccact tctgaaatcc
      721 gctgatgaaa tcttatttct tgtttgtcaa agtcaatatc tttttcttct aaagctgcaa
      781 tttctccttg tcttgctcct gtaataaatg ctagaacaat taaagtttta atttctatgc
      841 tttcctcaaa tgctgctttt agcattgcat gaatttcttc ttccgaataa ggttcttcga
      901 tattcttttt tacgcctttt tcttttggta tttttacatt ttccacagga ttttctttaa
      961 ttgctccgta ctcattttta gccatcaaaa ataagttgtt aacggcgtat aacatacgaa
     1021 gctttgtatt tcttgataaa ggtttatcag agtttctccc aggatctttt gtattgattc
     1081 tttgagcatt aacaacaatt ttctttatca tgtatggttt gatatcgatt agagggataa
     1141 aatcaaattt atcaagaaat ctattctcga tcattcgctt atagttatga taagactttg
     1201 gctctaaatt tttttcggcc tctgaaagcc atatttttct ataaaattca ccaaaagtta
     1261 tattgaacaa atccaacgaa tcatcggtca ttccttcgaa ttgctctaac cagatgttca
     1321 attctttata tgccgctctt ttactacttg cttttatagt tttgcttttt ctttttgccg
     1381 agccatcagg tttgtatcct aatacagctc ttagtctata tgtttcgttg tctacttgtt
     1441 ctaagtatcc tgttacttct gacataattg aatcagctct ctttctctga tacaataggt
     1501 actataaaga agcctatcgt ataggtttgt ttctttctta gaacacgctc gctttggtcg
     1561 gtggggcgtg ttttttatac taattagttg tgaagagcaa ttgaaactgt ctctgttcca
     1621 aagtaacctg tttgtgcttg caatacatta ttttttgcat ttgcttgggc ttcagagata
     1681 tcaaaaacta ttttaccagt ttgttgcata tcaggattta agctttccat aaagaaagag
     1741 ttagtgatat taccattttc atcttgattt gccgacatag atgcagccga atcagcttcg
     1801 aaagtttttc catctgcttt taatttgaag aatgagctat caacagtcac agccttatca
     1861 cctgcatttt ttacagataa gtcaactact aagaaagtgt cttttgcgtt agtaggaagt
     1921 acagatgggc caacttgttt tactacttca acggagttta ctttgtattc cattttccct
     1981 acagcaacgt catcaccgat tttataagtc ttttcttctt ttgttgtagt ttcttttgta
     2041 gtttctttat tagtacttgt tgatgttgca gtagctgaat ctttgttttc accgccactt
     2101 agagcaccac caataccaaa aaacaaaagt actattaata cccaaaacca tacccgttta
     2161 taaaacggtt ttttaacctt atacattttc ccatcttgac ccataacttt ttttgccatt
     2221 taaatattcc tcatttcttg ttataatata tttgtgatct cagaaatgag gtatgagtcc
     2281 gtgttgcagc acgggctttt ttatttataa cttttttaga gattataggc aaaatagtag
     2341 ggcataaaaa tatattattg aattccgtat ttaaaaaatc ctaattgaac ttcaccggaa
     2401 gtcttttgct gtgttgtacg caatgcttct tcttcagaca ttccattctg tactttccat
     2461 gcaacaggcg acatcccgta tttgttaaca aaatcagtaa gtgataaagt gtcagcgtct
     2521 tgctgagcgc ttgtttgttg gtcttctgga ttttgttgag atgctgcttg ttgttctttc
     2581 tgatcttgac tgataatatt gccagcatca tctgtagtca atccattttc ataaagggcc
     2641 acgccgaaag cttcccactc tttgttggac caatttgcac gatcagctgg agttgactgt
     2701 aaagtgcgtt gtttcatctg ttcatatgtt tcttcttgag gtgcggtttg gattgtactc
     2761 tgactggagc ttataactgt tgggctaggt tccgccgtaa cttggttgga gcttgtaact
     2821 gttgaactag aatctgtttt agatgtagat ttgctagtag atgaactggt ttcagttgtt
     2881 tcttttgttt tacttatttt tgtttcttgg ttagaagtgg catctgttga ttcagctttt
     2941 ttattatttg aacaagctga aagtagcaga gcagtactta acaacaacat aacgctaact
     3001 tttttcattt tatactcctc ttttctttga tacaataagt tgtaaagaag cctactatag
     3061 gtttaacatc ccacgtccac aaactttggt cggtgggcgt gtttttttgt ttatagactt
     3121 tcgaaaacta aagtagcttg gattctatcg ccaccaccga aacctttact accaccgttt
     3181 gtagtggaaa tagtgtgtaa tcgataacct ttagaacact gtctatttat agtgttttca
     3241 agttctgata ggttttgcga cccttttcct ataaactttt cttttaatac aacttgcaat
     3301 acaacgtaac ttggcatatt cttttccttc tttctactat gataataaat actaacccct
     3361 agccggagcg ccaactacga ctggggtatt ttttagaata cgactttccc gattattcta
     3421 atctgttccg gtgagacgac tctatcttca tataattcgt ttatagagcg tagaataatc
     3481 gtttgactct cataatcctt atatacttct ttacacgtca caccgtcacc atctatttca
     3541 acgatagcaa tctcaccgtc ttccacgtct ggctgttcct taacaaatac aaattgtcca
     3601 ttcctgataa ctggttccat tgaatcgcct ataataggaa cagcaaaatc agcattgttt
     3661 ggaactcttt caaattcttc ttcctctaca actgtatcgc cataaactaa ttcagtcggg
     3721 ttggcggcag aagctgaatt tttgattgtc ggaagttttt cctttttagg aaagtgcacc
     3781 acgtttcctc gattttgctc ttcgagttgc tgttcagcaa agttgtagac ttttgtttgt
     3841 cttggttttt ctaattgatt gtagattgtt tcaatggatg gaatgttatt tttcttatct
     3901 tctttaggaa catcgtaacc aagcaaccat gcctcactca catttaaagt tttagccaac
     3961 aaataaagct tgtgttggtc gggcgatgac ttaccattga catattgcga tagatgactt
     4021 tttgacattt ttattccagt ttctttttgc aaaggtaaag acatattcaa aatatcaact
     4081 tgctttaaat ttctttcaga cattaactga ttaagtcgtt gctgagtcgt ttgcttcatc
     4141 ttatacctcc tttctgatac ttattatata ccttattgaa cataagttca ataaaaaagt
     4201 taaattaatt tgaactttta tgttgacaat gttttatgca ggagttatac tttggttaca
     4261 aagttcaaaa catttgaact agaaaggagc gaactaatat gagttttgat tattctaagt
     4321 tgtcgggaag aattgttgag aaatatggaa cgcaatacaa tttttcaatc gctttaggtt
     4381 tgtcggaaag aagtttgtca ttaaagttga ataaccgagt accatggaaa agcaccgaac
     4441 ttcaaaaagc cattgaattg ctagatattc caaaagaaga aattggcgaa tattttttta
     4501 atttagaagt tcaaaatgtc tgaacttaag aaaggaattc gaaaaaatga acacacaaaa
     4561 atttgaaaac aatctatttg aattagaagt taagacggaa aatggtgaat cgttatttaa
     4621 tgctgaaaca gtagcaagaa gtttgggttt cgtggaaaca aaaaacggta aagaatatgt
     4681 tcggtggaga actgtcaatg gatatttaaa aaaatacctt tcgcaagaag ttgcgaaaaa
     4741 cgactttatc agtgagccaa tggtttacaa attagccttt aaagcaaaca acgtacttgc
     4801 tgaaaaattc caagactggt tagcaacgga agtcctacct caaatccgaa aacacggcat
     4861 gtacgcaaca gatgaactac ttaacaatcc agacttgctg attgaagtag caaccaaact
     4921 caaagaagaa cgcacgttac gattgatagc tgaacaacga gtaaatgaat tacaaccgaa
     4981 ggcagactat tacgaccgaa tcttgaacaa caaaggatta gttacagtta gtacgattgc
     5041 taagaactac ggtatgagcg ctgtatcgtt taataaattg ctacatgaat taggtatcca
     5101 gttcaatcaa agtggcacat ggctgctata cagtaagttc caagacaaag gatacacgca
     5161 tattgagcca tttgattatg aagataaaaa cggcaatcgg caagtaaaaa caaggatgaa
     5221 atggacgcaa aaaggacaca tcttcttata tgaaacattg aagaagaata attacttacc
     5281 gatgattgaa cgtgaacaaa cagcatagga ggtaacacta tgaacgagga aatgagccaa
     5341 acaatcaagg tacaaaaaat gatagatgat ctaacacatg caattgatag ccaagccgat
     5401 aaaatcatca aggaattgca aggacaaaaa gtttgggatg caaaaatgct tttcaagact
     5461 atcaaatttg aaatgaatcc aacaaaaaga aagctcgccg atgtgttgga agaaaagttg
     5521 gcttccgcta tcaacgagca agaattacta tttgaaaccg atacttttaa cagctgatgt
     5581 tttatagatg acagtttcat ccgaatctat atcattatag attcgattat tgcaatgtac
     5641 gaattataaa tacaggagga aataaccaat ggagcaacta gctttagtta acctctcaga
     5701 ccttaaagct tctttggcag aatctgagat tgcaaatgaa gtgtgggata caaagcaagc
     5761 tgcggaatat ttaaaaacca caacacgcac gctgactaaa gatgccgaat ctggaaagat
     5821 tccagcagcg aaagttggca gagagtggag gtttagtagt atcgctttgt atcagtatct
     5881 aaaaggagga aaaacatggg taaattcaac agagcactag tgttcagcgc acccctaatc
     5941 gtctatgctt taggactttg gggaagcaga caagcattaa tcggcacgat cgtttacatg
     6001 gtttggattt ttatggggct tgatgaagct gagtacagag cgaaaaagcc caccgagggg
     6061 gctgactaaa gtgtggttct gtttattagg tgtttatctc gtggccgttt tagtaaataa
     6121 ctacatgaga aaacgtggcg aatactggta tacatcctat gcggttctag tatgtctaat
     6181 gcttacaatt tttctaatga tttattcaaa ataggtaata ctttgtcaat caaataagtt
     6241 tcaagagatt tttgttctgc atcagtgttg ccgtaaccaa aacggcttaa gaaaattttc
     6301 ataacctcta tatcttcatt ttgaatatat ggcaggactg cataacctga tgcttttaca
     6361 gcagatatat ttttatcagt gttactacca atacaaatac ccacattgtt gagaaatgtt
     6421 gagaaagttg tttgtattgt ttgggttttt gaatcgtgtt tttgtaattc aatttcttta
     6481 tccttcatcg attcaacatg cttattattt ataaccgctg taatcgaagg ggatataaga
     6541 gctactaaag ctaggataat cgaaattgtg atcgtgttat cgaaactcat tttttcacca
     6601 tccagttttt aaaccattat atcaaaaagg agagaagaaa taatgcaaga attagtaatt
     6661 ttgaaaaata aaaaaagaca cgaacgccag caagcatgat cgtgtccaaa agaaaaatct
     6721 tttaaaccat tataacacag gaggaaataa atgatctcag ttaaagggtt aggcgatgaa
     6781 atattcgaag caatgatgag caaagcacaa caggacatca aagaaaaaat catttctgct
     6841 gcagcgcaag gacaaactag ctgcactgtt cgatcgagag gaacgacacc aagttttctt
     6901 ttggcgctag aagatgaagg cattttgaat atcaaacgaa gagatggcag cgtcagattg
     6961 ttttggcaat tttaggaggc cgtgatgaaa gattttgatt ctttaggcgc cagacagcaa
     7021 ccaccaaatg aagctagtcc tgttggagtt gattggcaag aaaatccgtt atatccaggt
     7081 gatacttgct atctaacaga agaaggctat gtaccagtag acgcaattct agagtacgtc
     7141 cagcaacatt atccaaagat tgaattagga ggaatttagg aatggcaaat gatttaacac
     7201 aaacaacaca acgttcatta gatgagcagg taatcagtaa tcttggacgg ttacaagaac
     7261 aaggtttaga aatgccacca ggatatagtc cacaaaatgc gttaaaaagt gcttttttcg
     7321 aactcactaa caatacagga ggaaacttgt tgcagatggc tgcaaacaat caagaaatga
     7381 aaacctccat ttctaatgca cttttagaca tggttattca agggttatct ccagcaaaaa
     7441 aacaatgcta tttcattaaa tacggaaaca aggttcagct aatgcgttca tattttggaa
     7501 caatggcggt ccttgatcgt gtaactggag gggcagatat tacgcctgta gtcgttcgac
     7561 aaggtgatga gtttgaagtt gcaatggatg gaccaaacat ggttgtcaaa aagcatgaaa
     7621 caaaattcga gaatttagac aatgaaatca ttgcagccta tgtagtcatc aagttaacaa
     7681 atggtaaaga gaccacaaca gtcatgacga aaaagcaaat cgatcaaagt tgggctaagt
     7741 caaaaatgaa agattctgga ccgcaaaagg aatttccaga agaaatggct aaacggacag
     7801 taatcaatcg ggcagctaaa acattgatca acacaagcaa cgacaacgat ttattagttc
     7861 aagctgcaaa agatacgtta gaaaatgaat ttgacaatga tcggaaagat ataacgccac
     7921 aaacagaaaa agtggctact ctcgaacaaa aattcttttc aaacaaaaag attactgaac
     7981 caatacaaaa agaaccagat ccgattgtga ttccagatga tatccaaaat gaagtaactc
     8041 gagttgctga tgtacctgcc catccagaaa tagagcaagc acatttgatc gaaaatgagg
     8101 atatcaatcc aattcaagaa gaattgatag atattccaga ctttggacgc gaggaaggtg
     8161 tagaagatgt ctcagaattt gaagacgacg agtacccttt ctgatgagaa ttattactcc
     8221 aatgaagctg actggcagta catgtcaaca tcacaatata agtccttttt aaaatgtgaa
     8281 gccgcagcct tagcaaagtt gaaaggcgat tgggcaccga cgtcggatcc caaagcctta
     8341 ctcgtaggaa actacgtaca ttcttatttt gaatcaaaag aagtacatga agcgttcaaa
     8401 gaagaaaata aatccaaaat gttttctagt aggaagccgt atggattgtt gaaagatttc
     8461 caaattgctg aacaaatgat tgaacgactt aagcaagaag aagcattcat gaacatttat
     8521 caaggtgaga aagaaacgat cgtgactggg gaactatttg gaacgacatg gaaaggcaag
     8581 attgattgct tgaatgttga ggatgagtat tttgttgata tcaaaacaac caaagacatg
     8641 catgagcgga aatggaacga aaactacgga tcaagagaaa cgttcattgt caattttggt
     8701 tacgtacttc aaatggcaat ctatcaagag cttcttttac agcaatacgg aaagatgttc
     8761 atgccaatca tcgctgcggt atcgaagcaa acgcctagtg aggcgaagtt gataacgatt
     8821 gatcaagata agatgagcta cgagctaatc atgctaaaag aaaatattga aagaattgtc
     8881 aaagtgaaaa atggcgaaga aaggccgata ccttgcggta cgtgcgagta ttgtcgaggt
     8941 aacctttcaa ttactggatt cactagcatg gacgatcttt aaaaggaggt gctttgcgaa
     9001 tgaatctagg atacatcaag ttataccgta aagtgactag ttcattcgtt tggaccaatt
     9061 ccgatatgtt caagctatgg atattgtgtt taatgaaggc tagtcatgaa gatagaaaat
     9121 ttttatttaa cggtcaagaa gtacgtttga caagcggaca attcgtcaca ggagcccatg
     9181 caatagcaaa agagtacaac gaaggagtcc cgagtgacaa agcaattgca tggcgaacgc
     9241 tatggagatg gcttaagaaa tttgaaaatg aagaattatt gacaatccaa tcgaacgcta
     9301 gatatagcgt tataacaata ataaattggt ctgactatca atcgagtgac aagccagtga
     9361 caagcgacgg acaatcgagt gacaagccag tgacaacaaa caagaatgat aagaatgata
     9421 agaatgataa gaataataat cctcgcaact ctcgaaaaac acgagagtat gcagatgacg
     9481 atccaaataa aaaattggcc attcttttat taaaactcat tcgaaagaat cgtagtctca
     9541 aagaacccga tttgaacaaa tgggccaata cgattcgttt gacgattgaa gctgacaaac
     9601 gaactggaaa agaagttcaa gacatgattg tttgggctac taaccatgat ttttggtcca
     9661 cagtcatttc atcgccaact agcttaagaa aaaatttcga tacaatggtg ggtcaaaaaa
     9721 ataaaagaaa gcaacaaaat atttctaatg atgagttacc agaaacaggt gaggattggt
     9781 aatgaataaa aaactaagtg caatggctgc accttacggc ggattaaaaa cagcagatca
     9841 taattgcccg aaatgtggtg atccattgta tatctggaaa acaaaaaata aagatggtac
     9901 tgatcgatgc ggtcctacat gtatcaataa gatttgtggt tatcgagaaa tggtaactaa
     9961 aaatcaaaaa gaagctatcc aaaaagcgaa tgaagcaatg aaaaaggacg ctatcaatcg
    10021 aatgcttaac agttcaatga ttacagatga tgccatatgg accttcgatt ttgatggata
    10081 caaagtagtt gatcaggaaa cagcacaaat aaaagcgatg gctcaagaat gggctaaaaa
    10141 aattgtaaat ggtagcacga ttcatgcagt gataaccggt agaacaggag ccggcaaaac
    10201 ccatctaggc gctgcagtga ttaaagaagt catgagggca tctaattata aaattgcttg
    10261 ttcatttatc agttatcgag aacttttaga acagttgaaa tttgcaatga atgatccaga
    10321 agcaagaaaa gccgtaacag gttcgctgat ggctgagata aagaaatcag atttcgtggt
    10381 aatcgacgat ttgggtgcag aactaggaag aatggaagaa aacaaccagg caacgcctta
    10441 tgatgttgat gttctcacat cgctcacaga agctcgtcta aacaaagcta cgatattcac
    10501 aaccaattta tcatcgaagc aattaaaaca cgcgtacggt gagcgagtgt tctctcgtgt
    10561 aatgaatgga acgaaaggga acatagctgt attcaaaaca acgacagaca aaaggaggaa
    10621 tccagtttga cctttgtagt gaaaaaaatg tgctacttag atagccgagg gcgaggagaa
    10681 gctagtgtcg agtttgctaa acaccacacg actaaagaag aggctgacct tgttgcaagt
    10741 gtttgtggcg gcgaagtagt cgaagttatt aaacccaaaa gacgattttc aaaacctaag
    10801 acaaagccag tgaaaaaaga gtgttgttgt ccgaaaagta accaagcatg gatgagaggt
    10861 gcaaaatgac ttgtttaaaa tgcaacgatg aaacagttat ttggtataag acattgcttg
    10921 gatggtcaac ttgtgaacct tgtccaatat gcaatgaaaa tggacgacgt tcgaaagaac
    10981 gactcgaaag actaaagaag gagtatagca aatggcaaca agaagcaaat acggaaacaa
    11041 aaagcacgaa gtagacggca tcacttttga ttctatcacg gaagcagagt attacgatct
    11101 agccttgtgg caagcacagg cgaatggttg gaaagtgaag ctccaggaaa ggtttgagct
    11161 gatgccgaag tttgaactag agggcaagaa gtatcgcaag atcgagtata ttcccgactt
    11221 cacattttac aaaagcggca agctggtcaa agtcgtagat gttaagggga tgcagacaaa
    11281 agactttaag atcaaggcaa agttgttctg tcatcaatat caagtgccgt tgatattagc
    11341 caaaaaatat cggaatacgt tcaaggaaga gcgtttttaa cgaggtggtc catcatgaca
    11401 acagaagaag tgattcaaat gcgaattcga agccttcggc gtgagattga cgatctggaa
    11461 cgaacaaagg cagtgatggt caatgaaacg gctagaaagg caatcgattt gcacatagag
    11521 aatttaagaa gggaaatccg tagattggag gaatgagcgt ggataaggaa gcggcttggc
    11581 gaaaattaat gttgctgatt caagatgaga actggcaaga agatgaagca gtggttgctg
    11641 aagttcagcg tctaggaaag attgctaacg gacgtatacg aaaaaagcca gacaaaagaa
    11701 aacagcgcaa agggaaaatc gtcgttgttt tacacgaagg caaaattttg atgcaaggaa
    11761 cagctagtga gctgtctgca gaaactggat atacgcgtgg gactattcga acgtacgctt
    11821 ggcgaaatca caccgatcga aaagggcacg aatataagta tttggaggaa gaaaaatgaa
    11881 cgaaaacaaa ttaatcaaat tgggtgtagc aggagcagta atcgtaggta ttggagttat
    11941 cggaggattt aagttcttcg aaaaaatcga taatggatat gtgggtgtgc gctattcaat
    12001 gaacggcggt atcaaagatg aagcactgac gcaaggtgtg aaatttgtag ggattgacaa
    12061 agtgatccaa tatccaattc gcttgcaaac tatccaatca aaaaatattt cagtatctac
    12121 aagcgacggc aaaaagacaa cgattgatat caaatatgac tacaaagttg attcaactaa
    12181 agcagcaaaa atgtacaagg aatttgggaa tatcacttcg gaagatatcg aaagtggatg
    12241 gttaaaatct aagcttcaaa aggtcgctcg tgaagtttat gcgaaatata gtctgcttga
    12301 tgtcctttca ggagattcct ctaaagttga agctgaggta ttaacgaact ttgctaaatc
    12361 agttgaatct aaagggtttg aagtcgaaga cgtaacactt ggtgttccag atgtcgataa
    12421 agaaacacaa aaatcaatcg atgcgatcat tcgagctggt caagaaaatg aaaaagcgaa
    12481 gctagacgca gaaactgcaa aaactcaagc tgatagtgaa gcttacaaga aaacaaaagc
    12541 tgcagaagca gaggcagaat ctaatcgcaa agtcgccgaa tcagtaacag acaatttgat
    12601 tcgttatgaa gaagctcaag ctcgcaaaaa gcatggatgg gtaacagtaa atggagcaga
    12661 tactgtagtt acggatgaag caggcaaata atatgggatt ctttatggct aaaattctct
    12721 tgttcttagg tttagtagga gcagcatatc tcgtgtatgc gctcctttcc caaactgatg
    12781 acaaagaaga tgacaacaac gatgaaatga aattttagga ggagaaataa tggacgaact
    12841 aatcacaaaa gtagagcagt gggctaaaga caagggactg gatcaagcag atccaaaagc
    12901 acagtttttg aaagtagctg aggaattcgg ggaaatagct tcggcgatgg caagaagtaa
    12961 tgatgaacta tttaaagata gcgtaggaga cgttatcgtc acgctgatta tcctttccat
    13021 gcaaaaaggg acaaacgtac aagagtgttt agaaatggca tacaacgaaa tcaaaggacg
    13081 cacagggaaa atggtagatg gtgtattcgt gaagtcgagt gatttggagg gcagcaaatg
    13141 ataaaaaagc tcattcaatt cagcatggat ttatatgata tcgaatcagg agcaacacta
    13201 tctgtggaat cggaccatct aatcataaat tttggtggaa agcgccagat tattttgtgg
    13261 gtagttgatg atgtactgtt tccagaaatt gttcatgatt tcgaagaatc aaaagcggtt
    13321 gagtttgaaa tagtgaaaaa agtaatggaa ttgattgaaa aatacgagga ggacagcgaa
    13381 tgataccgaa gtttagagcg tgggataaac gaaagaacgt aatgagagat gtagccgtct
    13441 tgcattttac taaaaacggc aaaacaaact ttattgaata ttggataaat cctaccgaat
    13501 tgaaatcata tcacgtgcga aacatcgacc tcatgcaatc cacaggaatg aaagataaga
    13561 atggtgtgga tatatttgaa ggggatatag tattagtcag cgtgcgaaat ggcttcgatt
    13621 acttagataa taaagtttgt attgtcaaaa attcaataga ttattccgga ttagtttgtg
    13681 ccactgttga tgaagactta gagtatcaaa tttttaacac agagctgttt gaagaataca
    13741 cgtatgaagt catcggaaat atatacgaga atagcgagtt attggaggta gagtgattat
    13801 cttagctact gactacatcg attctttaaa agatgaaaat gggaatgtcc cagcattgct
    13861 aaattgtgga attaaaagca agaatagaat cattttgtta cggatatcgg ctgatttagc
    13921 aaaatatctt tatctgagca agacagcagt atattacact gcggctacac gttatcagta
    13981 caagggaaaa acaatcagtc aggattatta tgatcgttta atcagtcctg atatgtatgg
    14041 gaattcagaa tcagcaatta agctgtttgg cgcaatagag atatatgccg atgattttcc
    14101 aaatttatgg ttgaaggagg aagcggaatg aaatacgaaa taccactaag tgaagcagac
    14161 gtccagtcaa ttattaacgg tcgggaggtt aataaaaaac ttcctgatgg tactgaatta
    14221 gtcatcagac aaagttattt gaaagatatg gcagctccag tattaattga tcgttttaac
    14281 gtgactgatt ctgtggtaga gaaccacttg aaagaatttc gatcaagtat aaacgacact
    14341 ttcagattag ggagttgatt gacaatgaac accagacacc gcagagtagc aaaactaaga
    14401 aaacaggaac tgaatgtact aaagacaaag tttgaaaaag aatatggaat ttcagcagaa
    14461 gaaacatata aagtggcaag tcagtgtgtt gctgatgcaa gtgatgctat tcgtaagttt
    14521 ggaatttcga tattaagtga tgatcgtaaa tgggaggaaa aagaatgaaa ctaaaagacg
    14581 gattttacgc tagcagtcac ggtatcggcg gtttaatgct agatatgccg acaaagaatc
    14641 ctaaaacacg taagaaacca aaagtcaaag tcggtgacat ggtccgctgt gaagcagagg
    14701 agttcattta tccatttcgt ggatatgtag aacacgtcta taatcactca gcgatcattc
    14761 gcattgaaaa cacgatggaa tgtgataaat ggacagctaa aagcaaagag aatttagcag
    14821 tagtgagatt ggtggatatg gaactaatca atgacaaata aaaaagccgg atcgctccga
    14881 ctaacataat aaaacagaca agtttattat atcacataaa aggagcagtt tgaattgatg
    14941 caattgttac gagaggtaga tttcaaacag acaagatgta atgcgagaga tgtgctgaag
    15001 aactttcggc gtttggagcg gatggcaggt cgctctttga tagatattaa gtcgccgatt
    15061 attacggata tgccgaaggc accgaagcac ggcaataagg cagaggatgc attgattcag
    15121 atgatggata tagaagcgga gagagacgcg attttagcgg ctttgatggc tcttagtctg
    15181 attagtcgtc agatactcta ctacagtttt tgtgtgccag acagcttctc aaactacaga
    15241 attagccgtg aagtgggtta ttcagaaaga agtatacaac ggatgaaatc ggaagctcta
    15301 atagagtttg cagaagcata taaacacgga agaataattg cttataaata gggggaccat
    15361 ataatgtgga aagattatgt atcgttgaaa gagttgaaaa aagatcttgt tttcaaaaga
    15421 attgtagaat ggtcagagag tgaattaatt ttagaagatg gaaccaaaat ggaagttgta
    15481 tgtagcgaat cagattgctg cgcatgggcc gaaggtgaat tcaaaaatgt aaagcttgat
    15541 gcagtgatta cagatataaa gatttttgat aaaggtaacc gtctctataa tggtgacgga
    15601 catacctctt atgctgaagt cgttgtttat cataatcgta atgagatcgc taaagcagaa
    15661 tgcacagcga atgatgggaa tggcggctac tattatagtg tttgtgcttt aaaggtcaaa
    15721 gacaaacttt gtatagtgac tgacgcataa aaaaatggcg gttttttggc ggaatgatgg
    15781 cggtttttag ccatttatca gtgatattat ggtagtgtcg aaagattagt gataggtctg
    15841 agacaaaata ataataaaag gaacatcgtt ttattattgt ttcacaatta agcttcgata
    15901 gacagcaacg gaaatattaa gaataaggat gtgaatttta actccttcta aattgttctt
    15961 attatctatc atccgttgct gtttattgtt acatacagat cgcttaggcg gtctttttat
    16021 tttggccgaa aacctacatt ttcgatggcc gattattgga ggaggaatag catgttcaaa
    16081 ctatctgaaa tcattaagaa gtccgacgtt gagaaattag agatgttaaa aaagaagttg
    16141 aagaaatagc agtctcttcg tgaggctgtt ttattttgct cacaaaaata gaccactatc
    16201 gggtaatagt ggtcaggaat taaatgaaaa agatgttagg gttgttagct aagtataaca
    16261 tcataacgct tacaaaacaa tgcaagaagg ataaattatt atgagaactt actggtatgt
    16321 gtcattaaac aataaatatc cgaagccaat gaaaggacag cataggcgtg tagtcatgtc
    16381 tgttcaaatg aaggcgaagt attcgattgt agaaatgcta agagaagcta caccaaaaga
    16441 gattgatcag tataagttgg ttcttattgg cattggttgg tttaaggatg aacatattca
    16501 aacaaacatg aagaggtggt tgagatgaag ttatattatg ttgaaaaagc aaattgttgg
    16561 gtggttgaag gctgtctgtc aaagtacgct acagatatta tatctactca attttcagag
    16621 ttgggattga aggttatctt ttgtggtgag tttgtcagtg tgaaagaagc ggtattgcca
    16681 atgacagcaa agagcagacg ctcgcgtggt tcaagtt
//
LOCUS       DAZBSA010000060        16375 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000060 DAZBSA010000000
VERSION     DAZBSA010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.060"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..150
                     /locus_tag="ACHGMT_001818"
     CDS             <1..150
                     /locus_tag="ACHGMT_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFN6835554.1"
                     /translation="HKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRH
                     SVIKG"
     gene            complement(435..1256)
                     /locus_tag="ACHGMT_001819"
     CDS             complement(435..1256)
                     /locus_tag="ACHGMT_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFN6835555.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(1353..1631)
                     /locus_tag="ACHGMT_001820"
     CDS             complement(1353..1631)
                     /locus_tag="ACHGMT_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835556.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            1899..3908
                     /gene="metG"
                     /locus_tag="ACHGMT_001821"
     CDS             1899..3908
                     /gene="metG"
                     /locus_tag="ACHGMT_001821"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFN6835557.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(4387..4974)
                     /locus_tag="ACHGMT_001822"
     CDS             complement(4387..4974)
                     /locus_tag="ACHGMT_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835558.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            5149..6471
                     /locus_tag="ACHGMT_001823"
     CDS             5149..6471
                     /locus_tag="ACHGMT_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFN6835559.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            6626..7402
                     /locus_tag="ACHGMT_001824"
     CDS             6626..7402
                     /locus_tag="ACHGMT_001824"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFN6835560.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            7395..7964
                     /gene="rnmV"
                     /locus_tag="ACHGMT_001825"
     CDS             7395..7964
                     /gene="rnmV"
                     /locus_tag="ACHGMT_001825"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFN6835561.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            7957..8841
                     /gene="rsmA"
                     /locus_tag="ACHGMT_001826"
     CDS             7957..8841
                     /gene="rsmA"
                     /locus_tag="ACHGMT_001826"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFN6835562.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            9292..9921
                     /locus_tag="ACHGMT_001827"
     CDS             9292..9921
                     /locus_tag="ACHGMT_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835563.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(10045..11154)
                     /locus_tag="ACHGMT_001828"
     CDS             complement(10045..11154)
                     /locus_tag="ACHGMT_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6835564.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            11363..11785
                     /gene="mgsA"
                     /locus_tag="ACHGMT_001829"
     CDS             11363..11785
                     /gene="mgsA"
                     /locus_tag="ACHGMT_001829"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFN6835565.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      11972..12070
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            12108..12644
                     /locus_tag="ACHGMT_001830"
     CDS             12108..12644
                     /locus_tag="ACHGMT_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFN6835566.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(12684..14447)
                     /locus_tag="ACHGMT_001831"
     CDS             complement(12684..14447)
                     /locus_tag="ACHGMT_001831"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6835567.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(14450..16165)
                     /locus_tag="ACHGMT_001832"
     CDS             complement(14450..16165)
                     /locus_tag="ACHGMT_001832"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6835568.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         5231 a         2989 c         3286 g         4869 t
ORIGIN      
        1 cataaattaa taacaaaatt gcgttataag tcccaatggt ataataaaaa atttcttttg
       61 atcaataccg acggagcaga agaaagcaat tccgtaagaa agagccaagt agtagaaaaa
      121 atgggcagac attcggttat caagggataa aaaatgaatg aaactgatag gagcgttagg
      181 gttagccttg ttaataaaaa gtcttaatga cttttgtttc taggaatttt tcttttgaag
      241 aaaagctcac taattatgac aaaccaataa aacaatagca gaaaaagttc gtacgagctt
      301 tttctgctat tgttaatttt ttattcagaa agtttacgaa tacatcaaaa ttctgaaaca
      361 aaaaacgtaa ctcacaaaga gttgtttttg cgttttttct tgaatgatta ggtctgaatc
      421 ttttgctcac aaccttattc atggtgcccc ggcttgagct gatagatttt tgaaggacgg
      481 ccgattccta caggacgttc accgatctca tcgaaatatt ggagcatagc tttcttaaaa
      541 ttggaatgat cgatcgtttt ataatcgatc cctaagaatt tagcaaatac ttttcgagct
      601 tccgtaatcg tgaaatcttt acctaaaact tgtagaactt gcggctcgtg ttccatttta
      661 ttgactacac ggttgaatgc tttcaagatg atttgactat ggtcaaaagc caaagtatct
      721 ttgccagaag actctccagt gactaaatca agcgagatag ccacttctcc gctagataaa
      781 tggatcttgt tactatgacg ttctagtgtg aaccagcgta cttccttggc atcatctccg
      841 gcgattagcg gttcttctcc aataaaggct aagtagctga ctgtgacgac ccagcctcgc
      901 ggatctcgat taggcgtact gaatgtatgc agttgctcaa tattttcttt tgaaataacc
      961 acaccagttt cttcctttgt ttctcttaag acgctttctc cagttgactc atccttttgg
     1021 acaaagccac caggcaatgc ccatgaattt cgataaggat gcccttttct ttggatcaac
     1081 aaaactttca gctgatcttc ttctttgtta tagcacatca gtacgatatc gacagtcaaa
     1141 gagggcttgt catattctgg acgttcctgt ttttgatacc agttaagaaa ttcttcttca
     1201 cttgctttat gttcataata taatttttct tcttctttag aagcaaaacg gttcatctga
     1261 ccacctccat tgacagtaaa agtacttttt accttattgt agcagactct gaaaaaaaac
     1321 caaggattta cttgaaaaat ctaggttatc atttaaatat gaaaaataaa tggaagtatg
     1381 agattgaata aaaagaaaag cagataaaac ggcaatactt tataccgctc ttcacgaatg
     1441 gcaaagaagc cacaaataac gatatatacg tcaatgataa aacgcaaaat ccaagctgct
     1501 gtcataaaac tgttcaaatg cccatggagg ttaaaataac catacatacg aatataaaaa
     1561 tagctgaata ttaaatgtgc taatgaaaga aaagcaatac taagtgtaaa aaaaagatgt
     1621 ttcttctgca tgattgttcc agtcccttac taagatagat agtctttatt ataccacgtg
     1681 tgaggggaga aataaaaagc cgttttttag tagatatgaa acaaaacaaa aacccgcatt
     1741 tattcaacct attcatcctt ttaaaggata gattgaacgt agatagaagt cgtttatcca
     1801 gtctttttca ttttgaaaaa aagctaacag catgcttttt ttatggtata ctagcaagga
     1861 aacgaattcg catcatgcga taaaggagga agagccccgt ggcagaaaag gaaacttttt
     1921 atatcactac gccaatctat tatccaagcg gcaaacttca tatcggcaac tcgtatacta
     1981 cgattgcatg tgacgcaatt gctcgttata aacggttgat gggctttgat gtgttttatc
     2041 tcactggagt ggatgaacat ggtcaaaaaa tcgaaacaaa agcagaagaa ctaggtgtaa
     2101 aaccgcaaga atatgtagat aaaatggcgg ctgatgtcaa gaaattatgg aaaacgcttg
     2161 atatcagtta tgataaattc attcgcacga cagatgatta ccataaagca gctgttcaaa
     2221 aaatatttga tcgtttatta gaacagggag atatttactt aggtgaatat gaaggctggt
     2281 actctgtttc tgacgaagaa ttctttacag aaacacaact tgcagaagtg tacaaagatg
     2341 acgaaggtaa cgtgatcggt ggtaaagcgc caagtggaca cgaagtagaa ttggtaaaag
     2401 aagaatcata cttcttccgc atgagcaaat acgcagaccg tctattggaa tactatgaag
     2461 aacatcccga attcatccag ccagaatctc gtaaaaacga aatgatcaac aactttatca
     2521 aaccaggatt agaggatctt gcagtttcac gaacaacttt tacttgggga gtaccagtca
     2581 agagtgatcc gaaacatgtg gtgtatgtat ggatcgatgc attatccaat tatattactg
     2641 cactaggata tggctccgaa gatgaaagtc tattcgaaaa atactggcct gcagatgttc
     2701 agatggtcgg taaagaaatc gttcgctttc atacgatcta ttggccaatc atgctgatgg
     2761 cccttgattt gcctctacct aaaaaagtat ttgggcatgg ctggctattg atgaaagacg
     2821 gaaaaatgtc taaatcaaaa ggaaatgtcg tttatcctga aatgcttgtg gaacgttatg
     2881 gtttagatgc actccgttat tatttgcttc gtgctgttcc gtttgggtca gatggtgtct
     2941 ttacgccaga agattttgtt tcccgtgtca attatgatct ggcaaatgat ctagggaact
     3001 tattgaatcg aacgattgct atgatcaaca aatattgtga tggactcgtt cctgactatg
     3061 cctcacttgt cactccattt gatagtgaac tgtctacgac ggctgctaac gttgtcggac
     3121 gttaccatga tgcaatggaa aaaatggaat tcaatacagc attggctgaa atctgggtgc
     3181 tgatttctcg tgcaaacaaa tacattgatg aaacagaacc atgggtatta gcaaaagacg
     3241 aagaaaagaa agctgaattg aatagtgtca tgatccattt agctgaatct ttgcgtatcg
     3301 cagcgatatt acttcagcca atcatgacag aaacgccaga gaagatcttc aatcaactag
     3361 gattggactc tgaaacaatg aatcttgaag gattacactt tggcgaattt ccttcaggaa
     3421 caaaagtagt tgcaaaaggt acaccaatct tcccacgttt agatatggaa gaagaagttg
     3481 cttttattca agaaaaaatg tctgaaggta ctcaaacaaa cgaagataca gtcaaatggg
     3541 atccagaaga aacagaactt gtttcaacga aagaaaaaca aatcaaattt gatgtatttg
     3601 aaaaagtaga actaaaagta gcagaagtca tcaactgtca aaaagtagaa ggggcagata
     3661 agttgctgca attccgacta gatgctggag acagccaaga ccgtcaaatc ttgtcaggaa
     3721 tcgctgaatt ttatcctgat ccaagcgaat tgatcgggaa aaaagtcgtc attgtagcaa
     3781 atctgaaacc acgaaagatg cgtggacaaa tcagtcaagg aatgatcctt tctgcagaag
     3841 caccagatgg ctctttacaa gtaatcgaag caccaaaatc catgccaaat ggctcagaaa
     3901 tcgcatagaa attcaaaggg ggtatgacac tagttgtgtc ataccccctt tttccgaaat
     3961 ttgagaagca attttcgtaa attccttctt attacttgaa gccaatcata taatacagaa
     4021 acagagaact atccaaaata aatcctagaa aaagaagtac gagcttcgct gatttttgtt
     4081 aatttagaat aatactaata ggaatcgtaa aaacagaaaa aaagcataat gataaagggt
     4141 cgggcagatt ttaattgatt catctggtac tcttttttat tgtccttaca ttcataaagt
     4201 ttgaatgcaa tgactttatt acatctatat ttatatctaa cagtagcgtt gatgtgttaa
     4261 ttataagttc tatcacttta tgttcaatta gtttgtgctc ttatatgtgt gattgaaaat
     4321 atagataaaa aaacaatcat cggctttgac ttcgatgatt gttttcttta ttttgtaaaa
     4381 atcctatcac ttgattttat ggtaaccttt tatttttgtc ttcaaacggc ttggtaagag
     4441 agcagtaagc agtgcaaatg ggatcgaaaa gacataccag aaaatcgtac gtccgatacc
     4501 ataatgttga tagagctttg tcaacatcac gtgtaacaag atcaacccta aaagcaagga
     4561 gccagctata aaccaatgat tatcccaatg catgacttca ctaagtgcca tcactccaaa
     4621 aggatagaaa taaaaataga tcccgactaa ggtcatccgc cagttctttg taaatgttcc
     4681 gatattcaat aaaaagaaaa tcgctgaaag caaaataccg ataggcggaa aaagcccaac
     4741 caaaaaggaa tacaacatcc cccacaagat catcacagga cctttgatca atgcaacgat
     4801 tgcgatgaac aaagtcagca atattagttt ttgtgataac gttgcaacag tcattaatcc
     4861 aaataaaatt aatgtgaacc aatgccaatg ggactgctgt tgttctttac gggaaaaatc
     4921 gacccggttg gataaataat ctgctaattt cggataagaa cgcttaaaat ccataaactg
     4981 ttcctccttc gtggaaaagg taggtttagt atacgaaaaa taaaaaagaa atacatccgt
     5041 cttaagaggg agtttttcac tgaaagtgga gaaaaaagaa tggtaaatta aaatattctc
     5101 agttatactg aaaattatac caaatttcaa agaagaaaga ggatgggaat gaaaggcaaa
     5161 ctaaaacgag aaatcaatct gtttggcgca ttagctaccg ttatgggaac cgtgatcggt
     5221 gcaggcgtct tcttcaaaac tgctgctgtt actgctagta ctcagtctac aagtttgaca
     5281 ttgctagctt ggcttttggg tggtttctta acgatctgtg ctggattgac agtggctgaa
     5341 ttagctacag caatcccgga aactggtgga gctgtaaaat atattgaagc tgcgtatgga
     5401 aaattgccca gctttttatt aggatgggcg caaagcttga tttactttcc tgctaatatt
     5461 gctgcattat cgattatctt tgcgacacaa ttgacgaact tattacaatt atctaccgat
     5521 tatcttttgt tgattgcagt gatcaccgct gtttctgtga cagggttgaa tttgttaggg
     5581 acaaaagtcg gtgcttctgt acaatcagtg acgttgattg ttaaattaat accaattgct
     5641 gtgattgtca tatggggatt attgacccca ggacaaggaa ctgtccagtt atttccaatc
     5701 gaagctggaa aagatgttac ttttgtcgaa ggattgagca gtgcgttgtt agcaacgttg
     5761 tttgcttatg acggctggtt aggtgtcggt gcaatggcag gggaaatgaa acggccggaa
     5821 aaagatttgc ctaaagcaat tattcttggt ttgagttttg taacagtcgt ttatctattg
     5881 atcaattttg tttttttgaa aactttgccg attgatcatc ttgccggtaa tttgaatgca
     5941 gcttctgaag catcagacgt catttttgga ggaatcggtg gaaagctggt cactattggg
     6001 atcttgatct cggtatacgg tgcattgaac ggttatacac taactgggat ccgcgtgcct
     6061 tatgcgatgg ctttagaaga cgagctacct ttcagtaaac agctgacaaa gctttcaaag
     6121 aagtttacgg ttccttatgt tcctgcggtt ttccagttag cagtagcttg tattatgatg
     6181 agccttggtt ctttcgactt tttaacagac atgttgattt ttgtgatgtg gctattcagt
     6241 ttactgattt ttatcggtgt ctttatttta agaaaaaaag cgccggaatt gccacgtcca
     6301 tataaagtac cactttatcc aatcgttccg attattgcga ttctcggtgc tattttcatt
     6361 ttaggtatga cgatgatgac gcaaacgaaa ttagcattga tcggtatagg tgttaccttg
     6421 ataggtatcc cagtctatta tcaaaaaaag aaaaaacgct cagaagagta aaatatttca
     6481 acaacagaca gaaaaagtac gaactaggtc ctctagatct tcgtgctttt tctatttcaa
     6541 taaaacaagt tcttctattt tgatttttgc tacttctgca agtagtgctt atgtgctaaa
     6601 gtaagaatga aaaggagaga taaacatgat atttgattct catacccatt taaatgctga
     6661 acagttcaat gaagatattc cagaaacgat tcagcgagca caagaaatgg gggtaacgaa
     6721 aatggcggtc gtgggcttcg atacgccaac gattgaaaag tctttgcaat taagccatga
     6781 ctatccaaat atatacagta ttatcggctg gcatccgaca gaggctggca gttatacgaa
     6841 agaaatcgaa aataaattac aagaacaatt gactttacca aaggttgtag cattagggga
     6901 aattggcttg gattatcatt ggatggaaga cccgaaagaa gtccaagata aagtatttcg
     6961 cagacagatt gctatcgcaa aagaaatgaa cctaccaatc agtattcata ctagagaagc
     7021 acttgaagac acgtatcaaa tcctaaaaga agaagatgtt cgagatatcg gtggaatcat
     7081 gcatagtttc agtggtgatt atgaatgggc aaaacgattt cttgacttag ggatgcatat
     7141 ttcatttagc ggtgtcgtca ctttcaaaaa agcattagac gtccaagagg ctgcagcaaa
     7201 tgtaccaatc gaacgattac ttgtggaaac agatgcccct tatctggcac cagttccttt
     7261 tagaggaaag cgaaatgaac caggctatac tcgttataca gtagaaaaga ttgctgaact
     7321 tcggaatctt ccgacagaag aagtggctgc ccaaacatgg acaaatgcgc accgattatt
     7381 tggtctaagc gaacatggat aagatgaaga tccaagagat tatagtcgtt gaaggaaaag
     7441 acgatacaag aaggctgcgg gaagtagttg atgcagatac gatagaaaca atagggtctg
     7501 caatcaatga agagatcctg atgcaaattg aacatgccca agagactcgt ggagtgatcg
     7561 tttttaccga tcctgatttt tcaggagaaa aaataagaaa aatcattatg gaagctgttc
     7621 ctcaggcaaa acatgctttt ttgccgcgca cacaagctaa accaaagaaa aagggaagcc
     7681 ttggtgtcga gcatgcaagt gatgctgcta ttttagaagc attgaaaaag gtcgtgacgc
     7741 cagacatttc ccaaacggct gtttctgaaa tcactagaca agacctttta gactatggtt
     7801 taatagctgg ggcacaagca aaagaaaaac gagaaaaact aggtgacgag ttgcgcatcg
     7861 gttacacgaa cggcaaacaa ttggaaaaac gtttgaagat gtttcgtatt acgccgaaag
     7921 aactcgccca agcaatgaaa atagtggagg aacaacatga gtgaatatcg tgaaattgcg
     7981 acaccctcta gaacaaaaga gattttattg aaacatggtt tttcatttaa aaaaagttta
     8041 ggacaaaatt ttctgactga accaaatatt ttaaggaaaa tcgtagaaac tgcgcaaata
     8101 gatgaacata cgaacgtgat cgaagttgga ccaggaatcg gtgcattgac ggaacaatta
     8161 gccaagcatg caaaacaagt cgtagcattt gagatcgatg atcgtttgat ccctgtattg
     8221 gctgatacga tgcaacccta tgacaatgtg actattgtac atcaagatat tttaaagacc
     8281 gatttgtcaa cagctgttag agaaacattt catgaggagc ttccgttaaa agtcgtagcg
     8341 aatttacctt actatattac gacaccgatc atgatgcatt ttcttgaatc agatttggtt
     8401 gtggatgaac tagtcgtcat gatgcaaaaa gaagtagctg atcgtatctc tgctgaacca
     8461 ggaacgaaag cttatggttc attatcgatt gctgttcaat attatatgga agccagttta
     8521 gcattcatcg taccaaaaac agtatttgtc cctcagccaa atgtagattc agcgattttg
     8581 aaactgacaa gaagagatat accggcagtc gaagtgacag acgaaaaagc atttttccga
     8641 ttgacaaaag cggcatttca gcaaagaaga aaaacgcttt ggaataactt gcagcattcg
     8701 tatggcaaag atgaccagac aaaagcatgg ttagcaaaaa gcttggaaac agctggaatc
     8761 gatccaaaac gtcgtgggga gactttgtca ttacaagaat tcgccgcatt aagcaatgcg
     8821 atgtcagaaa atatgcagta aaaaaagagg ttgtgacaga agttgtgaaa aagctgtcct
     8881 gcatcaagta ataagaacag ccaaacaaaa atagttcctc atgtttttcg tttgtttggg
     8941 cttattaccg cagatgcaag cttttgaaca ccgtttaaga aagaggttgt gggcctgccg
     9001 tttaactcta agtcacaagg aacaatttca aaaaacagct tctcatgttt ttgagttagt
     9061 tggtcttatt acttgatgca agcttttgac cactattatg ccgaacaagg agatgtgaca
     9121 caaattttgt cacatctcct atttttgttt cttgatcaaa aaattcttat acttgatact
     9181 cacactcatt ttgtatggat ttagaaaaga atatgtgtct tgacagttat tagtaagtga
     9241 gttatactaa gtcgacattt atgtgtcaag acatattagg agagagaaaa aatgagaaaa
     9301 aattcagttc gaaaactaac gatttctgct ttattgattg ggatgggggt cattatccca
     9361 atggtcatgc ctaaaatcat gattccacca gcttccttta ctttggcgtc tcatgtgcca
     9421 ttatttattg cgatgttttt tacaccagga gttgcagtgg ctgttgcgct aggtacgaca
     9481 tttggtttct ttttaacaac gccggttatc atcgcattgc gagcattgtc acatcttgtg
     9541 tttgcgttga ttggggcttg gtacttgcaa aagcatccga gtatcgtttt aaaaaatgga
     9601 cagtttactt ttgctaattg gcgtttccaa gggttcaatt ttgtgatcgg tgtgattcat
     9661 tctttagcag aaatgctagt agtcagcatc ttttacttta ttggtaatat gcctgaaaca
     9721 tattattctc aaggatattt ttatacagta ttcatcttga tggggattgg tgggcttatc
     9781 cacagtttag ttgattataa tattgcttat tttatagcag gaacactcag caaatatttt
     9841 gatatccccg tatttacggc agcaaaaaaa gaaaatcgga aagagtcgat tcaagggtac
     9901 gcgaagaaaa cgacatatta gacatgatct ctcaaaaaaa aaactctcat ctagtaatgg
     9961 ataataaatc ctctaagatg agagtttttt gtcctaaaaa gagctgcacc aacatcttct
    10021 ggcacagctc taacaggggg caatttatgg aagtttgatg acttgttctg tttctttatc
    10081 aaagaagtgg cctttgctga tgttgaaggc aaggtcgact atttcgcctg gtttatggaa
    10141 atctcttgca tctacttttg agatgaactc agtagaacca gtttttgtgt aaagcatcgt
    10201 ttcagctcca agtaattcgg aaacgacaac ttctgatttc actacggctt caggtgaagc
    10261 ttcaagggct acttgttcac tgtggatgtc ttctggacga ataccgaaga taacttcttt
    10321 tccttcataa cctttttcaa ctaatacttt gttttttcct tcaggaatac gaagcttaag
    10381 cccatgtcca tctgtgataa caccattttt caatgtcaca ttaaagaagt tcatggcagg
    10441 agatccaata aagccggcaa cgaacatatt gacgggtgta tcataaactt ctttcggtgt
    10501 accgatttgt tggataaatc cgtctttcat gataacgatt cggtcagcca tagtcattgc
    10561 ttctgtctga tcgtgagtaa catagatggt cgttgtttca agacgctggt gaagtttggc
    10621 aatctcagca cgcatggcta cacgtaattt agcatctaaa tttgataatg gctcatccat
    10681 taagaagact tttgcatccc gaacgattgc ccgtcccaat gccacacgtt ggcgttgtcc
    10741 accagataaa gcagctggtt ttcgtttcaa gtattctgtc agacctaaga tctctgctgc
    10801 attgtccaca cgttttttga tgtctgcttt atcatattta cgtaatttca agccaaaagc
    10861 catattatca aaaacagtca tatgaggata caatgcgtag ttttggaaaa ccatggcaat
    10921 atcacggtct ttaggtgcca cgtcattcat gacttttccg ccgatattta gttctccttc
    10981 agagatatct tccagtcccg cgatcattcg caaagtagtt gactttccac aaccggaagg
    11041 accaacaaaa acgataaatt cacggtcttt gattgataga ttaaaatcag ttacagaata
    11101 attttctgca ttgtcatatt ttttatatac gtttttcaga gccatttcta ccataatacg
    11161 ttccacactt tcttttttag ttttattaac tatttaaagt ataaatgaaa gcgttaacta
    11221 acttcaatgt catgttgaac aagaattttt tctttttttt gtcaatgtgc tagttttaag
    11281 ctttaataaa tatccataag aaataaaaac tgatacactg gagtaaataa gtatgttaac
    11341 aatatcaaaa ggagtgtctc ttatgaaaat cgctttgatc gctcatgacc gaaaaaaacc
    11401 tatgatggtg aaattagcaa cagcgtataa aacaatattg gaaaaacatg aactttttgc
    11461 taccgggacg actgggcaga ggatcacgga agcaaccggc ttgccgatcc atcggtttaa
    11521 atccggaccg ttaggcgggg atcagcaaat tggagccatg atttcggagg atcagttaga
    11581 tttggtgatt tttcttcgag acccgctggc tgcacagccc catgaaccag atgtaacagc
    11641 acttatccgt ttatctgatg tctatgagat tccgttagct acgaatatcg gaacagctga
    11701 ggtgctacta agaggactgg aagccggctt cctagatttt cgaagaatcg tccatgaaat
    11761 agacaagcat ccactttcat tttgatggat aagcgtatgg aaaaataacg tttaatgttc
    11821 gcctatatgt agctataaaa gctaaacaaa ctgataatat gcgatataat acgaacgtat
    11881 tggataatat aaacaataat attagttttt cgtatttgca gtaaatgtga aaatatgcta
    11941 tagtttctgt gggaaaagat cccaaaaata atacagagta gaaaataaag cgttaagtgc
    12001 taagaggatg ggatgttgcc tcttggacga aggacgtaat gttcgcggtt ttattttcgc
    12061 attcgctgca tgatacgatg tagcagctct taaaggagat gctaaaaatg aagaaacacc
    12121 ggttagacgc acgtatgatt gctattatgg gcttattgat tgcattgatg gtcactttgt
    12181 cccgactggt agcaatcgag accccattta ttaaaatcag tgtcacattt attccacaag
    12241 tgatcatggg cattcttttt ggtccttttt ggtcagggat aggcgcagtt ttagctgatt
    12301 tagtaggaat ggccctcttc tcgaaatctg cattctttat tggatttaca ttgaatgcat
    12361 ttattgaagg agcaatctac ggattctttt tctatcgaaa agagatcact tggaaaaatg
    12421 ccattctggc tacgcttagt gtcacgctga tcatcaactt atttttgaca ccattatggc
    12481 ttgcgttgat gtaccatgtg ccattgttca gctgggtcgt ttgggcgccc cgcctgttaa
    12541 aaacagtaat atggttaccg attcaatcaa ttgccattta ttacgtagga cgtagtattc
    12601 cttacaaaaa aattttaaga agtttagcga tccacgctaa ataaaaaaca agcagtcttc
    12661 ccatcccaga agactgcttg tttttattgt gctgtgaatt gtgcttgata gagccgtcgg
    12721 taataaccat ctttttgaag cagctcatca tgggtgccca tttcgacaat agctccctga
    12781 tccatcacta atatctggtc cgcatttttg attgtggaca ggcgatgggc aatcacaaaa
    12841 ctggtttttc cttccatcat tgctaagaag gcttgttgga tttttttctc tgtcaatgta
    12901 tcgactgagc tggtagcttc atctaagatc aacatagggg gattactgat catcgttcga
    12961 gcaatcgtca acagttggcg ttgtccatct gatatcttta acccttgacc gccaatctta
    13021 gtatctaatt tttccggcaa acgttctaca aattcataca tataagaggc ttttagggca
    13081 tcattgattt ctgcgtcact tgcttccgga ttcccaaaac gaagattttc tcgcaatgta
    13141 ctgtcaaata gccaagtatc ttgtaacacc attccaaaac ttttacgtaa tgtatcacgt
    13201 tggatagagc ggatgtcatg gtcatcgact gtaattgttc cttttgttgt gtcataaaaa
    13261 cgcatcagca aattaacaag tgtggatttt ccggcccctg tttttcctac tatagcaatg
    13321 gtttgacctg gataagcagt aaaattgaaa tcttcgatca aacgctgctt ggggtcataa
    13381 gaaaaagaaa catgatcaaa agagactttt cctttgatat ttttgggttc caaggcatag
    13441 gcatctggca aatcaggctt ttcttctggc tgatcgatca aatcaaatgc tcgttctaga
    13501 ccagaaaaag cggtttgcag ttgtgtcgtg attccagaaa gttcgataaa tggtttagaa
    13561 aactgacttg agtagatcgt aaagctggaa atcacaccaa cagtgattga cgaaccactc
    13621 ttcaatgcca ataggccgcc aactaaacca atcgataaat aagcaaggtg atctacaaaa
    13681 cgagaagacg ggtttgtcaa ggaagaagaa aattgtgccc gttgtccttt ttcatataga
    13741 tcagcattta gcagttcaaa ttgctgttga ctagcttctt cttgctgaaa agctttcacg
    13801 attttctggt taccgattcg ttcggtcaca aagcccgaga tatccccaat aatcctttgc
    13861 tgtctatcga aatttgtttg tgaagcacga gcaacgatcc aactcacaag gaaaataatt
    13921 ggtgtagaac aaagaacgac caatgtcaga agtggactta gacgcatcat aaaaataaac
    13981 gaaagaatga tgattgccac tccagaaaat aattgattga aggccgcaga aattgcaatg
    14041 gaaatattgt ccatatcatt tgtaaatcga ctgacgatat ttccgtggga gttttgatca
    14101 taataacgaa gtggcaatcg attcagatga gcaaatgcat cttttcttaa ttttgcaacg
    14161 gataagtaag ctaaccgatt gcttaacact tggatgagcc attgactgac tacgttgatt
    14221 aaaacgatac caccgaatag aataagtaaa tggaacagtc gattgaagcg gacctgtcct
    14281 tctccaatca tttgatcgat actttgacca atttgtaacg tcatcacaac tgttgcaatc
    14341 ccattgatga ttcctagtat cacagctagc caaatgtctt ttgcataggc agtaaggtaa
    14401 ggaatgaatc ggcggagcgt ctgcaaggat aattttttta ctttcataac tattcttcct
    14461 cctgtgatgc gacgatttcc tgatattctg ctgattgagc aagtaattct tcatgggtac
    14521 cctgagcagc aatcttacct tgatccatca ctaagatatg atgtgcttct tgaatagagc
    14581 ggacacgttg ggagataata atcactgtac tgccaagata agtctgtagc gccttacgca
    14641 aatccaagtc tgtttgataa tccaacgcac ttaaagagtc atctaaaatc agtaatgctg
    14701 gcttggcaat caaagctctt gcaatcgtca gtcgctgttt ttgtccgcca gagaaatttt
    14761 tcccattttc catgactttt gtatctaaac catttggaag ctgacggaca aattcttctg
    14821 cctgagcgat tttcagagct tcccaacatt ctttttctgt ggcgtctttc ttaccccatt
    14881 gaaggttttc acgaatagtc ccggtaaata gtacagaagt ttggggaaca agagcaattt
    14941 gctgacggag atgatccagc tgccaagaac gaacatccat tccgtccatt ataactgtac
    15001 cggaactgac atcatagaaa cgcggaatca attgtgtcaa agtcgttttc ccgcttccgg
    15061 ttggaccaat gattccaagg actgtatttc gcttcagctg aaatgtaata ttttggagag
    15121 aaagaccaga atcaggagta taacggaagt caacatggtc aaaagcgata attgcttcag
    15181 aagaaaaatc gggagtagta ctgacctcag cttcaagaat cgaattttct gtgtctagca
    15241 cctctttcac ccgatttcct gaagcttcgg cacgtgtgaa gatcacgacg aggtttgaaa
    15301 caacgatcag tgccaaaagc atttgattca tataattgat caaagcaaga acttgtcctt
    15361 gttgcaagct accaatattt actttgatcc cgccaactgt cagcaaacag atgatcccga
    15421 cattcatgat caaggttgta gcaggtgata atagggcaga gatattggaa acacgtaaat
    15481 aattgttcgc tagatcattt gtgcttttat cgaacttctc tgtttctttt tttgtctgcg
    15541 caaatgcgcg gatgacacga acaccgctaa gattctgact aacgtgttca ttgagctgat
    15601 ctagtttttc ttgcactttt tggtagagag gaacggtttt tttgatgata aaaaataaaa
    15661 tgattgagaa aataggcaat aatgctaaga aaaacagacc tacttcccag tcgatcacaa
    15721 aagccatgac cactgacccg atacttaaaa acggtgctct gatcaataaa cgaatgacca
    15781 ttgctaaagc tagctgaagc tgattgatgt cgtttgtcat tcgggtgatc aaggtatctg
    15841 ttccaaaatg attcaactcg ttgtgagaga aagtattgat ttttttgatt agctgattac
    15901 gtaactctgt accaaatcct tgtgaagcta ctgaagcata gtattggcaa atgatagcac
    15961 aaatcagacc aatcacagac atagcgacca tccataagcc catttcaatg atttttcctc
    16021 ggtcattcat ttttagtcca ttatcgatca aggaagccat cattaaaggc aggacaagtt
    16081 cgaagacagc ttcaagaaat ttgaagaacg ggcccaaaat gatttgctta cgatagtctt
    16141 ttgcataacg taataactga atcatatgat tcctcctatt atttatttgt ccacaattga
    16201 aaaaacaagt actaccacat tgtagcttag gaaatttaga atgaaaagag aacaagcctt
    16261 ttcataaatg aaagttttat tatgaagaag ctttagaaat tgaatagact atttttttta
    16321 aaaaaaagaa aatgagaaaa catgccagtc tcttcattca taggaacgtc tattt
//
LOCUS       DAZBSA010000061        16326 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000061 DAZBSA010000000
VERSION     DAZBSA010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.061"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            272..1129
                     /locus_tag="ACHGMT_001833"
     CDS             272..1129
                     /locus_tag="ACHGMT_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFN6835569.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1244..2164
                     /gene="pstC"
                     /locus_tag="ACHGMT_001834"
     CDS             1244..2164
                     /gene="pstC"
                     /locus_tag="ACHGMT_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFN6835570.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTITTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            2164..3048
                     /gene="pstA"
                     /locus_tag="ACHGMT_001835"
     CDS             2164..3048
                     /gene="pstA"
                     /locus_tag="ACHGMT_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFN6835571.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            3063..3869
                     /gene="pstB"
                     /locus_tag="ACHGMT_001836"
     CDS             3063..3869
                     /gene="pstB"
                     /locus_tag="ACHGMT_001836"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFN6835572.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3885..4643
                     /gene="pstB"
                     /locus_tag="ACHGMT_001837"
     CDS             3885..4643
                     /gene="pstB"
                     /locus_tag="ACHGMT_001837"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFN6835573.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4656..5333
                     /gene="phoU"
                     /locus_tag="ACHGMT_001838"
     CDS             4656..5333
                     /gene="phoU"
                     /locus_tag="ACHGMT_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFN6835574.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5910..6602
                     /locus_tag="ACHGMT_001839"
     CDS             5910..6602
                     /locus_tag="ACHGMT_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFN6835575.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            6599..7366
                     /locus_tag="ACHGMT_001840"
     CDS             6599..7366
                     /locus_tag="ACHGMT_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFN6835576.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7466..9034
                     /gene="liaX"
                     /locus_tag="ACHGMT_001841"
     CDS             7466..9034
                     /gene="liaX"
                     /locus_tag="ACHGMT_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFN6835577.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            9331..9651
                     /locus_tag="ACHGMT_001842"
     CDS             9331..9651
                     /locus_tag="ACHGMT_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFN6835578.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPFARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9651..10001
                     /locus_tag="ACHGMT_001843"
     CDS             9651..10001
                     /locus_tag="ACHGMT_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFN6835579.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            10168..11106
                     /gene="hprK"
                     /locus_tag="ACHGMT_001844"
     CDS             10168..11106
                     /gene="hprK"
                     /locus_tag="ACHGMT_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFN6835580.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANKNGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            11121..11951
                     /gene="lgt"
                     /locus_tag="ACHGMT_001845"
     CDS             11121..11951
                     /gene="lgt"
                     /locus_tag="ACHGMT_001845"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFN6835581.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11995..13020
                     /locus_tag="ACHGMT_001846"
     CDS             11995..13020
                     /locus_tag="ACHGMT_001846"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFN6835582.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            13035..13973
                     /gene="galU"
                     /locus_tag="ACHGMT_001847"
     CDS             13035..13973
                     /gene="galU"
                     /locus_tag="ACHGMT_001847"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFN6835583.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            14098..14538
                     /locus_tag="ACHGMT_001848"
     CDS             14098..14538
                     /locus_tag="ACHGMT_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFN6835584.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14541..15143
                     /locus_tag="ACHGMT_001849"
     CDS             14541..15143
                     /locus_tag="ACHGMT_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFN6835585.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(15202..16308)
                     /locus_tag="ACHGMT_001850"
     CDS             complement(15202..16308)
                     /locus_tag="ACHGMT_001850"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFN6835586.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNTSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
BASE COUNT         5292 a         2834 c         3532 g         4668 t
ORIGIN      
        1 ttctgagagt acgtcagaaa tggagaaagc gtctcgcatg agcgaaatta actttggggt
       61 atctcttgtt ttttggggaa agcaagaaat aagctaatat atgctattaa aagagacaca
      121 tttcaaagcc agtatagcat agaaactttt aatgagatag ttttttttac aattatgaga
      181 atttattttg ttatttaatg attttgattc gttttcatca agtaatgaaa atgataaaat
      241 aaacatactt taggattaaa ggagatttct aatgaaaaaa cttaaattat tcttaccaat
      301 aataggatta gctattcttt tatcaggctg ttcaaagtgg attgatcgag gagaatccat
      361 tacggctgtt ggctcttctg ctttacagcc tttagtagaa acagttgctg aaacttacca
      421 gacagaaaat cctggaaaat tcatcaatgt acaagggggc ggaagcggaa ctggtttgtc
      481 tcaagtacag tcaggagcag tcgacatcgg aaactctgat ttgtttgcgg aagaaaaatc
      541 ggggatcgat gcctcagcat tagtcgatca tcgagtagca gttgttggga tcactcctat
      601 tgtgaacaag gatattggcg taaaagacat cagtatggaa aacctgaaaa aactatttac
      661 aggcgagatc aaaaactgga gtgaagtcgg tggaaaagat cagccagtag ttatattgaa
      721 ccgcgcttca ggaagcggaa cccgtagcac gtttgaaaaa tgggtattgg acggagaaac
      781 agctgttcag gcacaagaac aagattcaag cggaatggtg cgccagatcg ttgccgacac
      841 acccggtgca atcagctatg ttgctttttc ttatgtttcc gatgatgtag atacgttgaa
      901 tgttaacggt gtagaaccga cagatgaaaa tgtgacaacg aacaaatgga cgatttgggc
      961 atatgagcat atgtacacaa gagatcatcc aaaaaaactg acaaaagaat ttttagacta
     1021 tatcttatct gatgaagtac aagataacat cgttggagaa ctaggttata tccctgtttc
     1081 tcatatgaaa gtcgaacgtg attggcaagg aaatatcgtt aaagagtaat ctttacacaa
     1141 aatttacaaa cttagctaaa cttttacatg ttattaaaac caaattgatg ttcaatttgt
     1201 acactgagtg agtatgttaa acctgattaa gggggagtca tctgtggaag atgtgaaaaa
     1261 agtattatta acaaaatcaa aacgttcaaa aatggaacag cgtggaaaat tcatcagttt
     1321 tttatgtatc gcgctgatcg tgttggttgt attatctatt ttttattttg tagctagtaa
     1381 aggattggcc acctttttta ctgataagat caatgtattt gattttctgt ttggtacaga
     1441 ttggaatcca agccaaatcg gagcaaatgg gaaaccaatg gtaggggcat tgcctatgat
     1501 cacaggttcg tttattgtga cctttttatc tgctatcgtt gccacgccat ttgccattgg
     1561 tgcagcagta ttcatgacgg agatctctcc taaaaacgga acgaagatct tacagccggt
     1621 catcgagctg cttgtaggta ttccttctgt cgtttatggt tttatcggtt tatcagttat
     1681 cgtgcctttt gtcagaagta tttttggcgg gactgggttt ggtatcttag ctggtacatt
     1741 tgtgttgttc gttatgatct tgccgactat cacaacgatg actgttgatg cattaaaggc
     1801 agtgccacgg cactatcggg aagcttcatt agcgcttggt gccacaagat ggcaaacaat
     1861 ctacaaagtc gtgctacgag cagccgtgcc cgggatttta acagcagtcg tttttggtat
     1921 ggctcgtgcg tttggtgaag ccttagctat ccaaatggtc atcggtaatg cagcattgat
     1981 gccaacaggt cttactactc ctgcttctac attgacgtcc atcttaacaa tgggaatcgg
     2041 aaatacaatc atgggaacag ttgaaaataa tgtgttatgg tcattagcgc tgatcttgct
     2101 attgatgtca ttattcttta atatcgtgat ccgtatgatt gggaagaaag gagagctgaa
     2161 ataatgaacg caaaacgctc agataaaatt gctacaggtg ttttatatat agtttcgggg
     2221 atcatcgtat tgattttagc agctttacta ctttatatct tagttcgggg aataccgcac
     2281 atttcatggt ccttcttgac cgaaccttcc aaagcctacc aagaaggcgg tggaatcggg
     2341 atccagctat ttaactcgat ttatttgctt ttgatcacga tgatcatcag ttttcctatt
     2401 tctttaggtg cgggtatcta tctttcagaa tatgctggaa aaaactggct aacagatgtg
     2461 attcgcacgt cgattgaaat cttgagctcg ttgccttctg tcgttgtcgg tttattcggt
     2521 ttcttggttt tcgttattca attccagtat ggtttctcga ttatttctgg tgcattggct
     2581 ttgactgtat tcaacttgcc tcttttgact agaacagttg aggagtcttt acaagcggtc
     2641 cattacacac aacgagaagc aggattggcc ttaggtttgt cacgctggga aacagtgata
     2701 aaagtggtgg ttccagaagc gacaccaggg attttgactg gtgtgatcct aagttctgga
     2761 agaatttttg gtgaagcagc tgcattgatc tatacggctg gacaaagtgc acctgcgtta
     2821 gattttacga actggaatcc attaagtgtt tcaagcccga tcagcatttt ccgtcaagct
     2881 gaaacattag ctgtccatat ttggaagatc aatacagaag gcacgatgcc tgatggtgtt
     2941 tctgtttcgg ctggcgcttc tgccgtgttg attattgctg ttttactgtt caacttcggc
     3001 gctcgtgcga ttggcaaacg attataccga aaaatgactt cagcctaagg agaagaagca
     3061 aaatgaaaaa atataactgg aatgaaacaa atgtcatcac catgccaaaa gagaaagaag
     3121 tagcactata tacggatgat ctacatgtct ggtatggcga caatgaagca atcaaaggag
     3181 tggatctgga atttgaaaaa aataagatca ctgcactgat tggtccttcc ggctgcggaa
     3241 aatctacgta tcttcgttcg ttgaatcgaa tgaatgatgg gatcgctaat acgaaaatca
     3301 ctgggaaaat catgtatcaa ggcgtagata ttaatgcgcc tcaagtggat gtttatgaga
     3361 tgcgcaaacg gattggcatg gtttttcaac gtcctaatcc gttcagcaag tcgatttatg
     3421 acaatattac atttgctttg aaacagcacg gcgaaaaaaa caaaaagaaa ttagatgaaa
     3481 tcgttgaaac aagcttgaaa caagctgcct tgtgggacca agtaaaagac agcttggaca
     3541 aaagtgcctt ggctttatct ggcgggcaac agcaacgttt atgtattgct cgtgcgatcg
     3601 cgatgaaacc tgatatcttg ctgctagacg agccggcaag tgctttggac cctatttcga
     3661 caggaacggt tgaagaaaca cttgtaaact tgaaagaaga ctatacgatc atcatcgtga
     3721 cgcataacat gcaacaagca gctcgtatca gcgactatac tgcctttttc tatttaggaa
     3781 aagtaatgga atatgacaaa actaacaaga tatttaccag accaaaaatt caggcaaccg
     3841 aagattatgt gtccggtcat tttggttaag acggaggcct caacatgaca aaagagatta
     3901 tttcatctaa agatcttcat ctgtattatg gaaagaaaga agcactaaaa gggatcgatc
     3961 taagcttcaa tcaaggggaa atcactgcga tgatcggtcc ttctggttgc ggaaaatcca
     4021 cttatctgcg ttctttgaac cggatgaatg acctgattcc gaatgtaacg atcaccggaa
     4081 gtgttttata taaagacaaa gacatctatg gtccaaaaac agatgttgtt gaactaagaa
     4141 aagaaatcgg catggtattc cagcagccaa acccatttcc tttttctatc tatgaaaatg
     4201 tcatctatgg attgaaatta aaaggggaaa aagataaaaa agtccttgat caagtagttg
     4261 aagaaagcct gaaggcagcg tctgtttggg atgatgtgaa agacaaattg cataaaagtg
     4321 ctttatcttt atccggtggg cagcagcagc gtgtatgtat cgcacgtgtt ttggcagtta
     4381 atccggaaat catcttattg gatgaaccaa caagtgcgct tgatcctgtt tctactggga
     4441 aaatcgaaag tatgctgtta gaattaagag agcggtatac gatgatcatc gtgacccata
     4501 atatgtctca agcttcacgt atttcagata agacggcatt ctttttagat ggtcacttga
     4561 tcgaatacaa tgatacgaag aagatattca tgaatccaga aaaacaagag acagaagatt
     4621 atatttcagg tcgatttgga taaggaggaa acggaatgct acgtacacaa tttgaagaag
     4681 aattgctgaa tctccataat cagttctacg aaatggggat gatggtgagc agcgccgtgc
     4741 acaagtcagt tcgtgcatac atcaaacacg ataaaatctt agcccaagaa gttatcgaaa
     4801 acgatataaa tatcaataac atggaaacac gcttggagaa gaaaagtttt gaaatgatcg
     4861 cattgcaaca gccagttaca actgatttgc gtatgatcat tacggtcatg aaagctagct
     4921 cggatttaga acgtatggcc gatcatgcgg tgtctgttgc taaatccacg attcgcgtaa
     4981 aagggcagac gagaataccg gaaatcgaaa aagagatctc cgatatgtcc gattatgtga
     5041 aaaaaatggt ggacaacgtc ctagttgctt atgtcaaaac agacgaaaaa gacgcacgta
     5101 cgatcgccaa tatggatcaa agagtcaatg actatttcaa tcgaatctat aatgcaacaa
     5161 tcaaaaatat gcaggaaaat cctgagacag tcatcagtgg aacagactac ttgaatgttg
     5221 ccagttatct agaacggatc ggagactatg tgacaaacat ctgtgaatgg atcgtctact
     5281 tggctacagg gaaaatcact gagttaaata cgaaccacaa tgagattttc taaactgaag
     5341 ctgtgattat gccgtttgtt taggaaaaga ggttgtgaaa tcagcggttg tgaaaaagct
     5401 gttctgcatc aagtaataag aacagtcaaa caaaaaatag ctcctcatat ttttcgtttg
     5461 gctggactta ttaccgcaga tgcaagcttt tgaacaccgt ttattcggga aagggtttgt
     5521 gacaaaacat gtcacaaacc cttttcatca tttcatagaa aggataaaga atgcaaaaaa
     5581 aaattagcat catcactata ttttttattt gtgtgcttac ttcatgggtt attcagcatc
     5641 aagccgcttt atatgaagta cctgtcatcg agatagaaaa agtacgaata aaagaggatc
     5701 atcaattgat tacaggaaaa ctgatgaaca cggatcaaaa aggagaatcg atttatttat
     5761 cggttcctta tcaaaaaaat caaatagaaa cagcaaatat aaaagcgaga caaatgtatc
     5821 tagtccaaat gcatggagat gaatggcagc tagtagcaaa aaaaaatgat ggctggcttt
     5881 tcttcttcac aagtatcttc attggcgcca tgttgcttat tggtggaaaa cagggtctat
     5941 ttgctttagg aagtctcatc atcaatatcg ttttactttt acttttgttg ctcatcttta
     6001 ctaaaacaga agggatttct ctattaaata tatctatcct gttcgtcgtt atagggatga
     6061 ttattagcat attggcttta gatggatgga atagaagcag tataatcaaa atttcagctg
     6121 cagttacgag tgttttttta gcatttttta tttgtctttt gacaatgaat cattggaaag
     6181 accaaggact gcgctatgag gaactgaatt atctgacacg accgtatcgt tctgtttttc
     6241 tttctagcgt actgattgga acagctggag gaacactcga taccgttatc acagtcattg
     6301 cgacattaga agaattgact aagcgacaac cagaaataac agcgaaacag ttatggcttt
     6361 caggaaggca agtgggtcga gatgtgattg gctcgatgac caatatcctt ttgttcgtct
     6421 atatcagtgg gtcagttcca tcgcttcttt tatatttagg aaatcaatgg tcgttcaaag
     6481 aaacgataga acagcattta tcattggaat ttttacgcgt cattgctggc agcttaggaa
     6541 tagtattatc gatccctatc tcggtcctct tttttctgac tgtaaggagg ctgaaacgat
     6601 gaatgtactt attatacttg ttgggatttt agcaatcagc gtgctactta ttggcggtac
     6661 gcaaggtatg tatattcttt taggcttatt catcaattta gggatctttt tcctgttgtt
     6721 attcggttat catcagaagt ggccgatact ggcattgtcg gtcataggat ttttattgat
     6781 tgctgtggtt atcttatttt ttatcaacgg ttataatcta aaaatgcgtg cagcttttgt
     6841 gagtatactg atctttttat tttgtttcct actattgctt cctatcactg attttttagc
     6901 aatccaaggt tttacatcgg tagaactaga agaattatct ggcttagata agactttagc
     6961 gattgatttt agattactta cgagatcgtt gcttctgatt agtcttagtg gtgctgtttt
     7021 agatgcgagt gtagcaattt ctagtgggac attcgaagta tatcaggcaa atccgcaatt
     7081 atctttttac caattgcgcc attctagttt cgctattgct aaaaaaatat tggcgtcaac
     7141 agtaatgaca cttctttttg cattcatggg aagttcttta gcgttgatcc tctggtttat
     7201 tgatttagac attccttttc agcaagtgat caatgaaaaa tcatttgttc tagaatatac
     7261 gatggctatt cttaccacat tgtccgcact acttgttctt cctttaacct gcgtgactac
     7321 agcatatctc tttacaaaaa agaaagaaca actcaagatg gagtaaaagt tacaacttaa
     7381 gtcctatgac atttttactg aatcagtgca taatatggat gtaaggaaaa gcttgcaaac
     7441 aattttgctt aaggaggcaa tttccatgaa agaaagagaa cgtattttag atttagtgaa
     7501 aaaaggaatc ctttcgactg aagaaggctt agatttatta gaaagcatgg caactgaaaa
     7561 agacgaaaag caaatcaaaa aagaagcaga caaggtaact gctagtcata aagaaaaaga
     7621 tcaggcaagt caattgatcg ataaacttga aaacggcgaa gaagaaattt ctgagccagt
     7681 tgatccaaag gaaagagaaa gacaagatca agaaaactta gaaaagatct tagatgaact
     7741 agcaactgaa gcgaataaaa cttcggcgcg tctagatgaa gtaaatgctc aattcgctga
     7801 caataaggca gcacgcaatg agaaacagga agcattgatg cagctgaata caaaagaaga
     7861 attaggcgag ttgacagaag aagaattagc acagcgtcag acgttagaag cagaaatcaa
     7921 agaattagaa gctagtggcg atacactgtt agaagaacaa ttgaaattag aagcagaatt
     7981 aaaagatatc cgtaagaatc aatggtctga gaaaaaagaa acgtttactg ataagtttga
     8041 attgccagat gattggaaag atcaagcaac agatacactg aatcaagtag gcgaaaaaat
     8101 gtcagaagct ggatcacagt taggcaaatt cttgaagaaa acattccaaa ctgtttcaga
     8161 aacagtgaat gacaacatgg aatggaaaga tgtcagctta cgtgtccctg ggattgcgac
     8221 aacgaaattt gagcatgaat tttactatga agctcctgca gcaagtatcc ttgatatcaa
     8281 ggcagcaaac ggaaacgtga cgttaaaaac gtgggacagc aacgatgtga aagttgaagc
     8341 taagatcaaa ctttacggaa aaatgggtgc tgaaccattt gaagcattct ctgaacggag
     8401 ccagattgag gttaatgaag atcatatctc cttccaaatt ccaaataaac gtgtgcgtgc
     8461 agaccttgtt ttctacttac ctaaacgagt atacgaccac gcagctatta aacttctaaa
     8521 tggcaacatc atgattgaaa cattggaagc taaagatatt tatacaaaat ctacgaatgg
     8581 taacatcatc gtggaccaat taactgcaac gatgctggaa gtagaaggtg taaacggcaa
     8641 tatcgatatt cgaaacggga acatcttaga ttctattatc gaaacagtaa atggtacagt
     8701 gacatttggt gcgacagcag aaaacctttc tgtgtctttg gtaaatggtg atgtacgtct
     8761 tacgattaaa gaagataatt tgaaaaaagt agaagcaagt tcagtgaacg gaaatgtcaa
     8821 agtcgcttta ccagatggca tcggtttgga aggacatgct aaaacaagtc ttggaagtat
     8881 caacagccgt ctgtccaatt acgaagtagt tcgtgagaaa aaagaacgga caaaccaaat
     8941 gttgcaattc cgtcgtgttt cagatggtga aatcgcgcaa gtgcaacttt caacgacaac
     9001 aggaagtatc tatttaaaag atacagataa ataaagaggt tgtgaaagag atgttcagcc
     9061 ttgagcaata agaagcgatt ttgtaaaata gcttccccta ttttacaaaa ttgtgcctta
     9121 ttgccgatag tctatctctt gaacaccgtt taccaagaga ttatgaacct gccaattatc
     9181 gccgagtcac aaggaataat ttcagaaaaa gctcgtcacg ttttatgaat atggtaattt
     9241 gtgaccgaga attttgcaga aagacaccga ttaagaagaa ggttgtcaca acgattttct
     9301 tcatttaaaa tagtagaaca gaggtgagac atgatgaaaa aaaagttgac aaaatctaat
     9361 aaaaatgttg ttttgacagg gactttagca ggaataggag aatacatcgg gattgatccg
     9421 actgttatcc gtgtgcttta tgtattcctg agcttagttg cttttggaag tccaattatt
     9481 ttatacattt tattagcttt gattatccca tttgcgcgtt ctgcacgaac aaactatgga
     9541 cataacaatg catattatga aaaaaacaat tatcgtgaac aaactcgtga acaaacaaaa
     9601 aaacgcaaag aagcggaaaa aatcgatgat gacgaatgga gtgactttta atgtcgtact
     9661 ttcaacgtat tttagttaat acgttaacat tcatatcatt ggctgttctt tttcctgcga
     9721 tggtccatgt gagcagtatt tggacggccg ttcttgccag cttcgtattg tctgtgttga
     9781 atatgtttgt caaaccgatt ttgacgatac tgtccctgcc ctttacatta gtcacattgg
     9841 gattgtttac gtttgttatc aatgcagcga ttcttaaaat gacctccttt tttgtgggag
     9901 cagaacattt tagtttttct tcattcggtg cagcgttgtt agtagctatc ttgatgtcgc
     9961 ttgtcaatgt aattgtcagc gaacataaca gtagaaaata aaagaaagct gtgccgaact
    10021 atcggcgcag tttttttatt tagaatcagg aatgcgtttt ctattacatt ggtaattaaa
    10081 taaaaacgtt ttttttaatt ggtagtactt ttggctagac aatgataaaa tgatagagaa
    10141 cactctaata gaaatgaggt agcatgaatg tcagaagttg taaaagttag ccaattagtg
    10201 aaaaaattat ctttggaaat cgtgacaggc gacgagaaaa gcttagaccg tgtcatcgta
    10261 actggggaca tctcacgccc aggactagag ttgactggtt attttaatta ttattcccat
    10321 aatcgtttac agctatttgg cagtaaagaa atcagttttg cggaacggat gatcccagaa
    10381 gagcgtttga tcatcatgag aagattatgt agtgaagata tgcccgcatt tattatttca
    10441 agaggattac cagctccaga agaaatgatc caggcagcaa ataaaaatgg gatagctgta
    10501 ctgcgttctc ccatctcaac ttctcgttta ttaggagaat tatccagtta tttagatggt
    10561 cgccttgccc ctcgaacaag tgtccacggt gttttagtgg atgtttacgg attaggtgta
    10621 ctgattcaag gtgacagtgg gataggaaaa agtgaaacag cgttggagtt gatcaaacgc
    10681 ggccatcgat tgatcgcaga cgatcgagta gatgtttatc agcaagatga attaacggtg
    10741 atcggagaac cgcctaaaat cctggaacat ctgattgaaa tccgaggaat cgggatcatt
    10801 gatgtaatga atctttttgg tgcgagtgcg gtacgtggct ctatgcaagt ccagttggct
    10861 gtttatctgg aagcatgggc aaaagataag aaatacgacc gtctgggctc tgaagacacg
    10921 accgttgaaa tcgcggaagt gggcattcca caaataaaaa tccctgtcaa aacaggacga
    10981 aacgttgcaa tcatcattga agtagccgcg atgaatttcc gtgcacgtac aatgggcttt
    11041 gacgcaacga aaacatttga agaaagatta agtcgcttga ttgaagaaaa ctcaggcaac
    11101 gactagcaag gagtgacaat atgttagcac aaatcaatcg agttgctttt gagcttgtag
    11161 ggattcctat ctactggtat gcattgatca tcgtatcagg tatcattatc gctatgtggc
    11221 ttagcagcag agaggctgtt cgggtaggat taaaagcaga tgatgtgaca gattttatgt
    11281 tgtggggatt gcctatctcc attattggcg ctcgcttata ttatgtttta tttgatcttc
    11341 cccagtatat agctgatcca atccaaatat tcaatatccg ttcaggaggc ttagctattt
    11401 atggcggact gattgcaggc gcgatcacgc tgtatttttt tactaaatat cattttattt
    11461 ctttgtggac ttttttagat atcgctgcac caagtgtatt attagcacag gcaatcggtc
    11521 gttggggaaa tttcatgaac cacgaagcat atggaccaga cacgacgcga agcttcttag
    11581 aaagtctgca tttaccgcaa ttcatcattg ataatatgta tatcgaaggg atctatcgtc
    11641 aaccaacatt tctttatgaa tcactttgga gtttgattgg atttattttg cttttgtttt
    11701 tacgaaagaa accacacctt ttgaaaaaag gcgaagtcct tttatgttat ctaatgtggt
    11761 atagtttcgg gcgtttcttt attgaaggat tacgtacaga cagtctttac tttttaggtg
    11821 tgatccgtat ttctcaatta ttatctgctg tgttatttgt cggtacattg attctattta
    11881 tttggcgcag aaaaaaagga aatttaccgg attatgaccg ctcattagga aaaaatcaac
    11941 aaattatctg atttttagag cgttaaaaat gaaaaaaatt tcaggagtgg taaaatgaat
    12001 tatagagtag cggttttagg accaggctct tggggtactg ctttggcaca agtcctcgcc
    12061 gaaaacggac atgacgttcg gatttgggga catcgtgctt cacaagtaga agaaatcaac
    12121 acccaacaca cgaatcaacg ttatttacca gatcattatc tacccgattc catccaagca
    12181 ttcaccaata tggaagatgc agtaaaagac gcagatgcgg tgctattcgt cattccaacg
    12241 aaagcaatac gttctgttgc aaaagaacta gtgcctaaat tgaatacaaa gccagtgatc
    12301 atccatgcaa gtaaaggatt agaacaggga acacataaaa ggatctctga catcttgtta
    12361 gaggaaatcc cagcagaaaa aagaaaagct gttgttgttc tttctggacc aagccatgca
    12421 gaagaagtag caaaacatga catcactacg atcacagctg caagtacaga tgcaaaagca
    12481 gcaaaatatg tacaagagct gttcatgaat aattattttc ggatctatac caatcctgat
    12541 gtgatcggtg tagagatggg ggcagctttg aaaaatatta ttgctattgg tgctggcgct
    12601 attcacggct tagggtttgg cgataatgca aaggcagcaa tcatgacgcg cggattagct
    12661 gagatcagcc gtttaggtgt agcaatgggt gccaacccgc tgacctttat cggattaagc
    12721 ggaataggcg atctagtcgt tacttgcaca agtgttcatt ctaggaactg gcgcgcaggc
    12781 aacttgttag gacaagggca gcccctagaa gaggtgctag ccaatatggg aatggtcgtt
    12841 gaaggtgtga atactaccca agcagcgatg gaactagccc aaatgatgga aatcgaaatg
    12901 cccatcactc aaacaatcta tgaagtgctg tatgaacaaa aagaaatcaa aacagccgca
    12961 aaagaaatca tgttacgtga tggaaaaaaa gaaaatgaat tctcattaac aattttttga
    13021 aggagcgaat gtatatgaaa gttaaaaaag cagttattcc agcagcagga ttaggaacaa
    13081 gatttttacc agcaacaaaa gctatggcaa aggaaatgtt gccaattgtt gataaaccaa
    13141 caatccagtt catcgttgaa gaagcgttga aatcaggtat cgaagacatc ttgatcgtta
    13201 caggaaaagc taaacgacca atcgaagatc actttgattc aaatattgaa ttagaaacaa
    13261 acctaagtga aaaaggaaag accgaattat tgaaattagt agaagaaacg acagatgtca
    13321 acttgcattt catccgtcaa tctcatccaa agggtttagg acatgccgtt cttcaagcaa
    13381 gagcctttat tggaaatgaa ccatttgtcg ttatgctagg cgatgatata atggaagacg
    13441 aagtaccatt gacacagcaa ttaatcgatg attacgaacg tactcatgct tctacgatcg
    13501 ccgttatgca agttcctcat gatgaaactt caaaatacgg aattatcaat ccaggagaag
    13561 tattggaaga tgggttatac aatgtcaaaa actttgttga aaaaccagat ccaagcaaag
    13621 caccaagtga tttagctatt attggacgtt acttattgac tccagaaatt tttgatgtat
    13681 tgtccgagca acaaccagga gccggaaatg aaatccaact aactgacgca atcgatacat
    13741 tgaataaaac acaacgcgta tttgcccgta aattcacagg taaacgttac gatgtcggtg
    13801 acaaatttgg ttttatgaaa acaagtattg aatatggtct aacacatcca gaagtaggtg
    13861 cacctttacg tgagtacatc ctgtctttag gtaaaacatt agaagcagaa ggaagcaaga
    13921 tcgatcataa tccaaaagga ataacagaaa tcaaagaaga aaagaaaaaa taaccctttt
    13981 ttctaacgag cttcagctgc taattgaata aaaagtagtt gaagctcgtt tttatttgtt
    14041 taaaaaacac gaaaatgcta tccttataga aaagagagac aagaagggag tttgatcatg
    14101 tcaggaggag aaattgcagg attgatcgca gcaatcgcct ttgcgatact tgtcgttttt
    14161 atcgttcgtg tgttgttgca agtttctaaa actttagaaa aagtagatca gactgtagca
    14221 gaagcaaata cgacgatcga aatcgtcaca aaagacgtag atcttttgtc tagacaagta
    14281 gaaggcttac tggtcaaaag taatgaactg ctcttggata tcaatggaaa agtcgcaacg
    14341 atcgatccat tgttcacagc agttgctgat ttaagtgaaa gtgtatcaga acttaatcat
    14401 tctagtaaaa acatcgcgac gaaagtcggc ggaatcggta aaacgacagc taaagcgacg
    14461 gttgccggaa aagtcggtgg gacagcgatg aaatttttta aaaacaaaaa acatactgaa
    14521 acgacaggag gaaaataaaa atggctaaaa aaggtggatt tttattaggt gcagtagtcg
    14581 gaggcacagc cgcagcaatc gccgcgttat tgttagcacc aaaatctggt aaagaacttc
    14641 gggatgaatt agctgcccaa gcggatgatt tgatggatat ggcttcagat tacacagata
    14701 tggcgaaaga aaaatcgact gagttaacag gtattgcaaa agaaaaagcg caagatcttt
    14761 ctcaacaagc aggtgatcta gcaggtagtg tcaaacaaaa agtaagtgaa tcagcagatg
    14821 aagcaggaag tgaatctgac gatatcttgt taaatcttaa agaacaatca gctgacttat
    14881 ctaatcgttt caaaaaaacg gcagaagata tgaaaaaacc tgcagatgaa ttaggaagta
    14941 ttgctaaaga tacttcagaa gatatcatga ttgatgtaaa agacgctgct ggtaaagcag
    15001 gagatactgt caatgaagga atgtcagaag caaaaccagt tgcagatgac tcaaaagaaa
    15061 tgatgaaaat ggcaaaagaa gatgcgaaag aagctaaaaa agaaataaaa gatacaatga
    15121 cagattcatc tacaaatcac taaaatcaca aatttataga ataaaaaggt gggagcggac
    15181 tagctctcac cttttttact cttatttttc taaaacaagt aattcttttg gtgtagtcgt
    15241 gaatggcaca cagccatcgc ttgtcacata tacacagtct tcgatacgta cccctacttt
    15301 ttcaggaata tagattcctg gttcaagaga gaagcacatc ccttcttcga ttactaagtc
    15361 atttccttct acaagggaag ggaattcgtg gacggttgtt ccgatcccgt gacctaaacg
    15421 gtgattgaaa tattcgccgt agccatagct gttgatcacg tttcgtgcga tttgatcaag
    15481 ttcacctgct gtcacacctg gacggacggc ttcttgtgcg gcaagctgtg cttcaagagt
    15541 gatgttgtag attttttctt ggaagtcggt tggtttttga taagcaattg tacgagtagc
    15601 atcactgcaa tagccattcc aaacaacgcc aagatcgaat aacacaagtt cattcggtga
    15661 aatcttcgta tttcctggtg taccatgagg gcttgcagca tttgctcctg caaggactaa
    15721 tgtgtcgaaa gacatttgtg aaacaccttg ttttttcaat tggtattcaa tttctgcaat
    15781 gatcgcttgt tcagtcacgc cttctttcac tgctttaaag ccaatctcaa atgcaacatc
    15841 ggcccattta ccagcttcga tcagtttgtc tttttcctct agagttttga tcagttgtag
    15901 acgttggatg atggaagtta gatcagtgta agaatccgtt cccgtcaaga tatcagagat
    15961 agcatcgaag cgctcgacag tcaaggcgtt cttttcaatc cctacattgc caggctgacc
    16021 atagcgagca cgaagttctt ttccgatgat ctcccaaggg ttttcgctgt ctaaatagcc
    16081 acggacatca tacgcccaac tagtgttttg agcatcttca acttccaaag caggagtgaa
    16141 aaggaaagga tcttgttcaa gagggataaa aagagctagt acacgctcat gaggatcact
    16201 ttcgtatccg gaaaagtaag cgatgtggcc agggtcgctg atataagcca attcgacttg
    16261 gtgttgtgcc atccattgac gtagttcatt gatttttacg ttattcattt ggcaacctac
    16321 tttcta
//
LOCUS       DAZBSA010000062        16254 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000062 DAZBSA010000000
VERSION     DAZBSA010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.062"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..636)
                     /locus_tag="ACHGMT_001851"
     CDS             complement(<1..636)
                     /locus_tag="ACHGMT_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835587.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDL"
     gene            complement(815..1534)
                     /locus_tag="ACHGMT_001852"
     CDS             complement(815..1534)
                     /locus_tag="ACHGMT_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN6835588.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(1665..2816)
                     /locus_tag="ACHGMT_001853"
     CDS             complement(1665..2816)
                     /locus_tag="ACHGMT_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFN6835589.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(3033..3773)
                     /locus_tag="ACHGMT_001854"
     CDS             complement(3033..3773)
                     /locus_tag="ACHGMT_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN6835590.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(3777..4100)
                     /gene="rsfS"
                     /locus_tag="ACHGMT_001855"
     CDS             complement(3777..4100)
                     /gene="rsfS"
                     /locus_tag="ACHGMT_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFN6835591.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(4157..4750)
                     /gene="yqeK"
                     /locus_tag="ACHGMT_001856"
     CDS             complement(4157..4750)
                     /gene="yqeK"
                     /locus_tag="ACHGMT_001856"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFN6835592.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(4734..5384)
                     /locus_tag="ACHGMT_001857"
     CDS             complement(4734..5384)
                     /locus_tag="ACHGMT_001857"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFN6835593.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(5394..5705)
                     /gene="yhbY"
                     /locus_tag="ACHGMT_001858"
     CDS             complement(5394..5705)
                     /gene="yhbY"
                     /locus_tag="ACHGMT_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFN6835594.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(5721..6830)
                     /gene="yqeH"
                     /locus_tag="ACHGMT_001859"
     CDS             complement(5721..6830)
                     /gene="yqeH"
                     /locus_tag="ACHGMT_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFN6835595.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(6827..7354)
                     /locus_tag="ACHGMT_001860"
     CDS             complement(6827..7354)
                     /locus_tag="ACHGMT_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFN6835596.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(7534..8319)
                     /locus_tag="ACHGMT_001861"
     CDS             complement(7534..8319)
                     /locus_tag="ACHGMT_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFN6835597.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(8312..9181)
                     /gene="accD"
                     /locus_tag="ACHGMT_001862"
     CDS             complement(8312..9181)
                     /gene="accD"
                     /locus_tag="ACHGMT_001862"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFN6835598.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(9182..10561)
                     /locus_tag="ACHGMT_001863"
     CDS             complement(9182..10561)
                     /locus_tag="ACHGMT_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFN6835599.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(10569..10988)
                     /gene="fabZ"
                     /locus_tag="ACHGMT_001864"
     CDS             complement(10569..10988)
                     /gene="fabZ"
                     /locus_tag="ACHGMT_001864"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFN6835600.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(11013..11489)
                     /gene="accB"
                     /locus_tag="ACHGMT_001865"
     CDS             complement(11013..11489)
                     /gene="accB"
                     /locus_tag="ACHGMT_001865"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFN6835601.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(11492..12727)
                     /gene="fabF"
                     /locus_tag="ACHGMT_001866"
     CDS             complement(11492..12727)
                     /gene="fabF"
                     /locus_tag="ACHGMT_001866"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFN6835602.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(12746..13483)
                     /gene="fabG"
                     /locus_tag="ACHGMT_001867"
     CDS             complement(12746..13483)
                     /gene="fabG"
                     /locus_tag="ACHGMT_001867"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFN6835603.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(13495..14412)
                     /gene="fabD"
                     /locus_tag="ACHGMT_001868"
     CDS             complement(13495..14412)
                     /gene="fabD"
                     /locus_tag="ACHGMT_001868"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFN6835604.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(14519..14743)
                     /locus_tag="ACHGMT_001869"
     CDS             complement(14519..14743)
                     /locus_tag="ACHGMT_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFN6835605.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(14796..15758)
                     /locus_tag="ACHGMT_001870"
     CDS             complement(14796..15758)
                     /locus_tag="ACHGMT_001870"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFN6835606.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(15768..16208)
                     /locus_tag="ACHGMT_001871"
     CDS             complement(15768..16208)
                     /locus_tag="ACHGMT_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFN6835607.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         4280 a         3709 c         2787 g         5478 t
ORIGIN      
        1 taaatccaaa tctgtttcag gatattttaa tttgacaagc gaaataatca ttttcagttc
       61 ttccatgttt tctggatatt ctgggtcaaa taccttgcta tgacggccaa tcccgccatt
      121 gtcgatcaat cggtaaattc gattccattc aatgaattgg tcaattgatt ggaaattgct
      181 gattggatag ccgccacaat ccaaaatctc tgatacacga aatgctaggt atgcactatc
      241 gatcacttta ttttttttcg tgtctatctt ctcatttctt tcgataagaa ttttttcagg
      301 acgatgtttc aagctccact ctaatagtag aaagaaagcc tccatccgat actcttcttc
      361 aaataaccga aaggctgaat cagaacgacg aaaaagcagc ggaatcagat aattttgtct
      421 ataaatcagc attggtgcgc ggtccaccat acactctaaa taacggttca gataccctgt
      481 accgacatag tttcctaatc gatagccttc aacgccgaat cttgcgtatt tttgcgctaa
      541 ttttgaacaa tcagttacgt tcacagctaa ctcttctact ttgcggtaac cgcttaagat
      601 acgttcaaaa ggttctaaag tgatttttgc ttccatattc ttccccgtcc atcttccaca
      661 tacttcatat catttcacat gatacttatg ctctcatcat accacaggtt gaattagaaa
      721 agaaaataaa tcacacaaaa aagcaagaca atctgttagt gagattgtct tgcttttcga
      781 aaaagactga atacttttcg ttaaaactgg ttgcttagtc ttctaatgcc acattatgga
      841 acacatgctg tacatcttct agatcttcca gtgcatcaat cattttttca aatgtttctt
      901 gatcttctgg agatagtgtt acctcgtttt gagggatcat ttggatctca gcgattgaaa
      961 aatcagtgat tcctttttct tttaatgctt cttgtatact atggaaatcc gctacttcac
     1021 cgtaaacgat gatttgtcct tcttcttcca ttacatcgcg aacatcgatg tctttttcca
     1081 ttaggtattc aaggatctcg tcagcgtctt ctcctgcaaa agcaaagatt gctgtattgt
     1141 caaacatgta agaaaccgct ccggaaacac ccatgttccc gccatttttc ccaaatgctg
     1201 cccgtacgtc tgctgctgtt cgattcacat tgttcgtcaa tgtatcaaca atcaccattg
     1261 agccgcttgg tccaaatcct tcatagcgca attcagagta tgtttcatct cctgcacctt
     1321 ttgctttttc gattgctcgg tcaataatat gttttggtac attataagtt ttagcgcgat
     1381 caatgacgaa acgtagtttt tggtttgcat gcggatcagg atcacctgat tttgctgccg
     1441 cgtagatttc aatcccaaat tttgcataaa tccggctatt attagcatct ttagcagttt
     1501 tctttgctac gatatttgcc catttacgtc ccatattttc acttcgcttt ctgatttgat
     1561 agtttcataa cgcaaaaacg tcctgttcca ttatacttgt gaatcacgat tctgtgaagc
     1621 tttttttctt tcttccttca ttcttctcat ttttagtcaa ttatctatac tcggattggg
     1681 atttgcccga aattctgttc cggaatattg cctcccaatt gatagatttg atcacttcta
     1741 actaacagtt ccgcttcttg tgcctctttt tttccgattc gggaataaat agaaatctct
     1801 tttttttctt ttaagaatct tcttccagca tcggtaaatc caagtagtcg aagataattt
     1861 ttattttgtt ggacaggaat ctcttgcttt ttgatattca gcaagacata acaagataat
     1921 cgttggatcc gtgtccatgt atatcttttt gtttttacct ttgacacaaa atcctcaaaa
     1981 ctatcagcag ttgcagctgc tgttttcatg cgcgattcta ttccttcggt catctgatag
     2041 atatctgtca gttccagcaa agtagaagat aacaaccgat aacgcaaata cggccaataa
     2101 gattcccaag tctgcagcga acaagttgaa agatcttctt ctgtctgagg cggcacaagt
     2161 ccagctattc tttttccttc ttctaacgca tggcgaatgg ctgttgcact ggctatcctc
     2221 tgatctgtga tgattttttc atgatagcct gcacttttac gtcggatcgg atgtaattcc
     2281 atagggtgga catacttggc attttccttt gcataactta atcccaagat atgatttggc
     2341 gaagaaaaat caagcgtaat atcgggatac agactgcgaa aaacctcagt cattttttgc
     2401 ggataactat acgtttgatc agacagttct tgaaatgcct cttctatcct atcttgattc
     2461 tcttggacaa agcgtccgaa actgctgtaa tcaaacgaag tctcttcttc tgttccaaaa
     2521 cacaaactgt cacagtccag tgtttgcaag atttttactg cacctttagc aaaatagtca
     2581 gctgactgaa ccgcccattc gacgggaagc tccactacta aatccacacc attttgtaaa
     2641 gcttcattcg cgcgatgcca tttatcgatg atagccggtt cgcctctttg caaaaaattc
     2701 ccgctcatta cagcaatgac gacctctgct cctgttattt ttcgtgccat ttccacatga
     2761 tagcgatgtc cattgtgaaa aggattgtac tctacgataa tcccgcagct tttcatgcgc
     2821 atcctccttc atagttatcg ataaaaaaga aatagagaca agtaaaaaaa ggcaaaaatc
     2881 ggaaaaaata cccgaactat ataaactatt ctcgcaaaca caatacttct gcgacgaaaa
     2941 gctgcactgc aagagcaatg cctatatcgt tcggatatat aatttatgtt gaagatttat
     3001 tcttttcttg ttaactcgag caggcaaaac cgctagtctt tgtatgctac aaagaaccag
     3061 cgctttgatt tctctgatgc tgcttcgtct gtaaaatcag aaaatgcttc gactttcgaa
     3121 aagccgctgt tttccagcat tcttaaataa ctttccaatg gataggtacg ctcttgatgt
     3181 agttcatctt cacgaataaa tcgaccagac ccttcttgtt cttcaacgaa aaaggtaaga
     3241 aaatgttcga tgctgtgttt ctcgcttcct ggataactat cccacaaaaa agcaaaatta
     3301 tccccttgat aatgatagct atattctgga aaaacctcat ctacttgata ggtggagtgt
     3361 acgtcaaata aaaatcggcc attttcttct aacgcttgat agacttcatc aaacacttgc
     3421 tgtacctctt gtctattttt catatagcac agcgaatcag aaaaacaggt aattgcctga
     3481 tattgtccga tttctgataa atcgagcata ttgccttcca caaattggat cgatgcgtct
     3541 tcttcatatg ctcgctggct ggcaatcatc aacatctctt ctgaaaggtc taaccctgtc
     3601 actttatatc catcttttgc aaaggcaacc gctaatgctc cagtaccgca tgccagttcc
     3661 aaaatatttt ttgtgtcttc ttgtaaatga cgtttcgaaa aagctaacca ttgctgataa
     3721 agtgtttcgt ccatcacttc atcgtaaatg aaagcaaatg tttcataagc catatgttaa
     3781 tccaaccatt cactaagatc gactagcgga gcatctgacc atagtttttc cagattatag
     3841 aaatttcgtt ctggcgcatg gaaaacatgt acgatcacat ctccaagatc gatcaatacc
     3901 cattttccgc cttcttttcc ttcaatacgt tttacttcat atttgttttc ttcttcttta
     3961 tcaatgattt cttctgtaat tgcgttgatt tgacgttcgc tatttgctga acagatcatg
     4021 aaataatctg ccaacaagga aacatttcga acgtctaaag cgataatgtc ttctgcccgt
     4081 tttgaatctg ctgcttcaat cgcaattttt aattgttctt tactatctat gatgatttcc
     4141 tcctaatatg agcagcttat ttttttgcta cccagtaatt atatgtttcg attgtttttg
     4201 gataaatctg ttgttcttgc gcaagcaaat gggccaatgt atgttttgtt tcaaatgcta
     4261 cagcttcgtc tagatcagcc catgcaatgg ctcgtgcatc ttgtacccca ggaaaatctc
     4321 gacctggttc aatatagtct gctacataaa tgattttgtc aaggagactc atcttagcag
     4381 ccccagtcgt atgcacccgg attgcatgaa gaatctctgc atcggtgatt cctaattccc
     4441 gctctacaaa cgaagcgcca acaagaccat gccagattgc attattataa ttcaataact
     4501 ccggatcaaa accgtctctt acgattacca ttttgaactc ctcatctggt cgttcttttg
     4561 cataatcatg tgtcaatgca gcaatactag ctttttcagg agaagcaccg tattttttgg
     4621 caagagccac ggctgtttct tctactccaa gcacatgctt gaagcgttgt tcgctcattc
     4681 tcatctgtat tttttgcatc aggttttctc tagaataagc agtatattca ttactatagt
     4741 ccatattcat acaacccttc attttgaata taatcaatta ctttatctgg aacaaggtag
     4801 cggatagaac agccttcttt gatcttttga cgtattttcg tggaactgat gtcgatctca
     4861 ggtacatcta cccaaattac aggatatgga gtatccgttg tgtatcctgg tcgacgtata
     4921 cccacaaagt tgaccataga agtcagctca tcgattttat accattttgg caaatattct
     4981 accatatccc caccgataat aaagtaataa tctgtatccg gattgttttg cgtcaattcc
     5041 ttcatagtat cataggtgta acttttccca ccacgagcta attcaatcgt ttcaatctgc
     5101 aaaaaaggat tgtcttccac cgctaattcc aacatgttga gtcgatgttt tgcatcaatc
     5161 gtctgtttct catcaacatg aggtggctga taagaaggca tcaaaaatac gcggtcaaga
     5221 cctaaattcc ggccagcttg ttcagccatc actaaatgtg caaggtgaac gggattgaat
     5281 gttccgccca agatccctac ttgctttttc ttctctaaga attgaggcat ttcttcagga
     5341 aataattgtg aacgaacaaa tgtcttagct tgcgtattca tcatggtcac tccttatatt
     5401 gcttttactt cttttgatag cctttgatac ttttcttttg tggaaggttt gtataataca
     5461 ataacacgtc caatgatttg gacgatttcg caatctattt ctttttctaa tacttgagct
     5521 gcttcttctg caatctcatc tgtattttga agtaacgata ctttgatcag ttctcttttt
     5581 tctaaagctt cttcaatttg aacgatcatt gctgaattga tcccaccttt tccgatttga
     5641 aaaatcggct gaagatgatg cgctttgcta cgtaaaaaac gcttttgttt tcctcttaaa
     5701 ctcataattt tccttcttcc ttaaatcaat gctttgcgtt tcaatacatc tacacccttt
     5761 ggcgcccagc ccgcaattac tactggcgct gttacagtga tccagcctag accggcaaaa
     5821 acgatgtctg ttttttcttt cacagaaaac tcaaaacgaa caagctccgg aaatgcctcg
     5881 acttcatcag gacgaggagg ctgtaataat ccacctacat gtttttgata aaattcgtct
     5941 gctttttcca gtttcgttcg atgaatattt acatcatttg acacataagc aataaatgaa
     6001 gcacgattgc cgctgataaa atcaaatctt gcaagtccgc ccaagaaaag tgtttgccct
     6061 tcattcagct gataaacttt aggtttgatt tcttttaacg gtgcaatgat ccgcaaatct
     6121 tttttaccta agtagtgcgc catttgatgg cgatggataa ttcccggtgt atcaatcaaa
     6181 aaatgcccgt catctaaagg aatctcgatt ttatccaagg tcgttcctgg aaactgcgaa
     6241 gttgtgatta catcttgtac tcccgctgta tttttgatga tttgattgat aagcgtcgat
     6301 tttccgacat ttgttactcc gacgatatag acatcccgtc catcgcggta ttcttcgatt
     6361 ttttctaata gttccgtcat ctcatgagat ttcttagcac tagttaataa tacatctact
     6421 ggacgaattc ctgcttcgta agcacgttcc ttcatccatt gtgttaattt agtttttttc
     6481 aatgatttag gtaatatatc cactttgttc ccaactaaaa gtactggatt gtcaccgaca
     6541 aaacggtgta gtcctgggat tagtgaacca ttgaagtcaa aaatatccac cacattgacg
     6601 atcaatgcat ctttttgccc taattcattc agtaagcgca aaaagtcgtc atccgtcaat
     6661 gagacatctt gggtctcatt ataatggcgt aatctgaaac aacgctgaca atagacttcc
     6721 ccggaagcta aacctttttc caaagccgtt tgcggcgtgt atcctaattc accgggtttg
     6781 tttgtttgta taacggcacc acagccgata cattgtaatt cttctgtcat tcgattccac
     6841 ctcgccagcc catatcaggg tgttttgcta atagataggc cataattctt ttttcccaaa
     6901 aacgattaaa ttgtgtcttc caactatctg ttgcgataat gggttttaca aggatgctac
     6961 gtatccctgc acgattggct ccacgtatat cagtcataat ctgatcgcct accattacta
     7021 tctcatcatc tgctaaatcc aattgtttct ttgctatgtt gatccctctt gctaaaggtt
     7081 tcattgctcg tgcaacatac aaaagatcaa atttttcaat cgctcgcgcg acacgttttg
     7141 aattattgtt tgaaacaaca acaacaggga ttcctgcatt tttcatttct aaaatccaag
     7201 taagcaactc ttctgttcca tctggattat tccatgcaat caacgtattg tctaaatcgg
     7261 tcaatactgc tttgatgcct agattcttta attgggcagg tgtaatctga tagatggctt
     7321 ctaccatcca tgttggtttg taatttgaaa acatgctgtc tcctcatctg caaaaaatga
     7381 aggagcacat aaagtactcc tcaaccccgt attatactgc atatcagcaa aaaaaactac
     7441 ctctatactc gttcgtcttt cagaaaaagc atttctttac aaaatgacca aaaataaaga
     7501 ttgatcacag aaaaatttag aaacaaaaaa caactaatat tggcggaacc tcttgtatcg
     7561 tttttccagt aatttatgct gatctaatcg aacaagttcc gtcaatcttt caatcaacgc
     7621 ttttttcata atgtgtgcga ctttatatcg tggcaactgc tcgccgttga acgtttcagg
     7681 aataactcga tcaacgattc ctaactcgcg tagttctggc gcagtaattt tcatcaactc
     7741 agctgcttct gcggcgcgac taccgtcttt ccacaaaatc gacgcaaatc cttctggaga
     7801 aagcacagca taaatcgttt cttccagcat ccagacttca tccgctaaac ctagagcaag
     7861 ggcaccgcca cttccacctt caccaatgat gatggcgatg atcggtacct ttaaatcaga
     7921 catttcgagc agatttctgg caatagcctc tccttcgccg cgctcttctg ctcctacacc
     7981 gcaataagca cctgctgtat tgatcaaggt aatcaccggt ctattgaact tttccgcttg
     8041 tttcatcagt cgtaaagctt ttcgatagcc ttcaggatgg ggagaaccaa aatttctgcg
     8101 gatattttcc tgcagatccc gtcctttttg aataccgatc actgttaccg gtttgccatc
     8161 taatcgagcg attccaccaa caatcgcttc gtcatctgca aataaacgat caccatggca
     8221 ttcctcgaaa gaatcgaaga tctcttcaaa aaaatccaag cttgtccatc gttctttagc
     8281 tcttgctaac tgaacgacat catgtgctgt cttatccacg atctatccat cctttctgtg
     8341 tatgcaattc aagaagcgta tggattttct gtttcaattc catgcgggga acaatctgat
     8401 cgacaaaacc atgagataat aagaattcag ccttttggaa atcttctgga agttcctgct
     8461 tgatcgtctg ttcgatgacg cgtcttccag caaaaccgat caagctttgg ggttctgcta
     8521 gaataatatc gccttccatt gcaaaactgg cagttactcc tccggtcgtc gggtccgtta
     8581 aaacagttag ataaaaaaga ccttcattgc tatgccgttt gacagcagca gagatttttg
     8641 ccatctgcat tagagaaaag attccttctt gcatccgtgc gccacctgaa gctgtgaaca
     8701 agacgactgg aagactttcc tttgttgctc gttcgaataa tctggtgatc ttttctccga
     8761 caatcgttcc catacttccc atgataaaat taggatccat cactccaata atgaagggaa
     8821 tcccctgaat ggttgcttta ccagtgagga ctgcttcatg aagtcctgtt ttatcttgca
     8881 tttttttaat tttttccgta tattcgggaa atgctagcgg atctttggtt acaagatccg
     8941 tatcccattc ttcaaaactt ttttcatcaa taatcaaagc tagacgctgc catgcaccga
     9001 ttcgaaaagc gtatccgcaa tgagggcata ctttttcttc accgatgtct ttcgtataaa
     9061 ggatatgttt gcaatttgga cactttgccc acatgttatc aggaacagat ggcgagttag
     9121 caggtttgct ctctctatta ggattgatcc gaatatattt ctttttctta aacagagcca
     9181 tctatgcgcc tcctatctcc gtttctggtg tccaattcgg taaaaattct tcttgtaaaa
     9241 atgctgtgct gtaatcacct gctatgactg caggatgact aatcaaatcc atctgaaatt
     9301 ctgcgttcgt gatgattcct tctgtgataa attcacttaa ggctcgctgc atcttcatta
     9361 atgcttcaaa acgcgtattt ccatggacga ttatttttgc aatcattgaa tcataatatg
     9421 gcgggatcgt atatcccggg taaactgcac tgtctactcg cagtcccatc ccacctgctg
     9481 gaagcagtaa gttttggatc ttccctggag aaggagcaaa atggaaagca ggattttctg
     9541 cattaatccg gcattcgatt gcgtgtcctt ggaatactac ttcttcttga cacacattca
     9601 aaggctcacc ggctgctatc tccacctgtt tttttactaa atcgattcct gtgaccattt
     9661 ctgttactgg atgttccact tggattcgag tattcatttc catgaaataa aaatcgcctt
     9721 cttcatccat cagaaactcg atcgttccgg cattttcata attgactgct ttagctgctc
     9781 gaacagcagc gtctcctagt gcttctcttt ttgtttgtga gatcactaca gaaggagatt
     9841 cttccagtac tttttgattg ttgcgttgta aagaacaatc tcgctcgcct aaatgaatga
     9901 catgaccaaa tcgatcacct aagatctgaa cttctatatg gcgagcagga taaatgattt
     9961 tttccaaata catatcatca tttccaaatg ccgctgctgc ttcttgctga gctgaagaaa
    10021 aatgctgtgg gagctcttct tttgattgaa ctttccgaat gcctttcccg ccaccacctg
    10081 cagctgcttt cagcatcact ggataaccaa ttctgtctgc tatttccaat gcttcttcta
    10141 ctgtagataa tacaccagta cttcctggaa tgactgggac atttgcttct tgcatcaatt
    10201 gacgcgcatt gatcttattt cccattgcat cgatcgttgc acttttagga ccaataaatg
    10261 tgatattgca ctcttcacac atggaggcaa atcggctatt ttcagataaa aaaccaaatc
    10321 ctggatgaat tgcttcggct tttgtcacga ttgcagcgct cagtacttcc tgaacattca
    10381 gatatgaatc aacggctttt gccgggccaa tgcagattgc ttcatctgct aactgtgtat
    10441 gaagtgcttc ggcatctgct tctgaatata tcgctacggt ttggatccct agttcgcggc
    10501 atgcacgaat gatacgtaca gcgatttcac cgcggttagc tatcaatact ttagaaaaca
    10561 tatttcacct atccaatcat aaaggttaat tccgcttcgg ctactttttt accgtctact
    10621 ttagcgattc ctttgcctat tccggctgaa gctttcactt tcaatatctc aacttcaagg
    10681 tataaggtat ctcccggtgt aactttctta cggaattttg ctttgtccaa tccgccgaaa
    10741 taagccgtct ttcccttgaa ttgttcaaga gaaagtaatg caactgctcc tgcttgagcc
    10801 attgcttcaa caatcaatac ccctggcata acaggttctt ctggaaaatg tccttgaaaa
    10861 aagggctcat tgatcgttac atttttctta gcaacgatcc gttctccttc aaccatttct
    10921 tccactcgat caatcaaaag cattggataa cgatgtggaa tgatttcttt tatttcttgt
    10981 atgttcattg ttttccctct ttcctgcttt gcttattttt gctgaatcct aaagagtggc
    11041 tgtccatatt cgacaactga ctcgttttct acaaggattt ctgtgatcac tccgtcggcc
    11101 tcactagtta tctcattcat cagtttcatc gcttcaataa tgcagataac ttctcctttg
    11161 gcaacttgat cacctacttt tttgaaagct ggtttgtcgg gcgctggttg cagataaacg
    11221 attcccacga ttggcgaaac aacctcgatt ccatcaaata ctgccgattc tttttctttc
    11281 attatttctg gagcagttgt tgcttgttca ggtatgacat tgactggctc cgaaactggc
    11341 aacgtttcgt ttgtttgaac catattcatg cctcgacttg ttttattttt attcatatac
    11401 aattcaaatt gaccttcacg caaatcgaat tccgttagcg aggattgatc aaattgactg
    11461 actaattctc tgatttcatt gatatccaaa attattcctc ccagcgtttc atgcacagaa
    11521 ccgcattatg tccgccaaac cctaatgaat tactcattgc ataagtaaaa cttgcaggtt
    11581 tgctttcgtt tttgacaact gtcaagtcaa tctcaggatc ttgtttttct acattgatcg
    11641 taggtgggat aaattgatgt tctaatgcca gcaaagttgc caaggcttcg atcccgccgg
    11701 ctgctcccaa taaatgtcca gtcatcgatt tcgtgctgct gacgtaagta tctctgaaat
    11761 tttcacctaa tgcgatttgg attgcttttg cttctgcctg gtcattggct ggtgtacttg
    11821 ttccgtgagc attgatatat ccgacttgtt ccggctgtac accagcttct tccatcgcaa
    11881 gcttcattgc tttagatgca ccagaaccat ctggagtagg agctgtcata tggtaagcgt
    11941 cacagtttgc accgtatcca acgatttctc catagatttt ggcaccgcga gcttgtgcat
    12001 gttctaaaga ttcaagaact agaacgcctg ctccttcacc catcacaaaa ccattccgat
    12061 tttcgtcaaa cggaatcgaa ccacgattag gatcagttga ttctgagagt gctgttaaag
    12121 ctgcaaatcc agcaatcccc atctcacaaa tcgttgcttc tgttcctcca gcaagaatga
    12181 catcagaata gccatgtttg atgttacgga atgcttcccc aattgaattc gttccgctag
    12241 cacaagctgt tacgatagct gtacaaatac cttttgcacc tacacgcaaa gcaatatttc
    12301 cagctgccat gttggcaatc gccattggta cgaacattgg agaaactcgt tttgcacctt
    12361 tttcatgtag acgaataact tggttttcga ttgttggaag accaccgatc ccagatccga
    12421 tgatgacgcc aaaccggtct tgattcattt tttctttatc taaaccgctc atctccattg
    12481 cttctaaagc agcgtagatt ccatagatcg aaaacatatc cattcgtttg ctatcttttt
    12541 taataaaata tttatcgtaa gggaaatctt tgacttcacc cgctaaggta atccctgttt
    12601 ctgaagcatc aaatttagtg atcggtgcga tgccattcgt cccctctttt aagctattca
    12661 aaaagctgtc tgcgtcattt ccaataggtg aagtgactcc atagccagta attaccactc
    12721 gattcatttg tttgctctcc ttttcttatc cgtgcatgac caatccgcca tcgacattga
    12781 tgacttgtcc ggtgatatat ggattttgtg ctaagaaaac tgctgtttgt gcaatatctt
    12841 ctacttgtcc gaaacgctgc atcggaattt gcttttccgc ttgttccttg actttgtctg
    12901 ctaaaacttc cgtcatatca gttgtgatga atcctggcgc aatggcatta catgtaatgc
    12961 ctctagttgc agcttctcgt gccacagatt ttgttaatcc tactacgcca gctttactag
    13021 ctgcataatt tgcttgacca atatttccaa tcaacccaga tacactagag agattaatga
    13081 tggccccttc tcgctgcttc atcatttttt tcaatacgtg ctgcgtcata ttgaaggttc
    13141 caatcaaatt gatatccaga cattttttga aatcttctgc atccattcgc atcacaagtt
    13201 tgtcatttgt gattccggca ttgtttacta acacatggat cgatccaagc ttttcttctg
    13261 cttctttgat catttgtccc gctttctcat aatcagagat gtcaccggaa actcctacgc
    13321 attttacacc gaatgcttcg atttcttcta tcttttcttt tggaatctct ccgcgaccat
    13381 tcaaaatgat atttgctcca gcttttgcaa aagctaaggc catcgctttg ccgattcctc
    13441 ttgtactacc tgtgataaat acattttttc cttttacatc cataacgttc ctccttatag
    13501 tattgctatt gtttcatcaa atgtctgctt attctcaaca cgcagaactg gtattgtttt
    13561 atcgattttt ttcataaatc cgcttaatac ttttccgggg ccgacttcga tgacttgttc
    13621 gatgccgatt gtttttaact tatggatact ctcatagaaa cgaacaggtt gcatgacttg
    13681 ctgttcaagt agtcccgcga ttgtttcttt cttcattatt tccgttgttg tgttgcttat
    13741 gataggaaaa gaaggttcag agaattgaat ctgacttagg tccttcgcca gtttttttgc
    13801 tgccggttct aaaatagctg tatgaaaagg cccgctgaca tttagaggga tcattctttt
    13861 gaaccctttt tcttttaata gtaaaacagc ttcgtctact gctttttctt cgccaccaat
    13921 cacgatctgc tgaggtgtat tataatttgc aggagatacg atgccgtact tgctagcttc
    13981 atgacagctt tcctcgatcg tttcaatcgg cgcattcatc actgcaacca ttttcccgct
    14041 tccagctggg gcagcttctg tcatataagc gcctcttttt gctactaacg caactgcttc
    14101 agtgaaggat agcgcattac tagctactaa tgccgaatat tctcctaaac tcagtcctaa
    14161 agctgcatct gggatgattc catgctcctt caataagcga taatacgcga tgctgactgt
    14221 tagaatagct ggctgtgtat attgcgtctg atccaaacgt tcattttctg taaagcaaag
    14281 ttcagccata tcgtagccga gaatctcact tgcttcatca aatgtctgct ttacaacggc
    14341 ttcttcatat aaatcttttc ccataccttg atattgtgca ccttggccac taaataagaa
    14401 tgctgttttc atgtttttct ccaatatatc cagaatatat gtttgattct tcctattcaa
    14461 aaaggccgag acattcctat ttcaggaaat gtcccagccc ccaacctaat tttaatgatt
    14521 atgcagtttg tttttctacg taagtcacta aatcttgaac cgttttgatt ccgtcttctg
    14581 tttcgatttt cacgtcaaat tcatcttcaa tttcattgat gatttggaat aaatccaagc
    14641 tgtcagcttc taagtcttct tggatgtcag tagtcaattt gatttcttca gtttcttttc
    14701 ctaattcttc tgcgatgatt tcttgaattt tttcgaatac cataataata tattcctccg
    14761 atattgtttg ctttttattt ttataattta acagtctaca actccatgag aatacttccc
    14821 catgttaaac ctccgccata gcctgtaaat attactttct gctgttttcc taaggtgatc
    14881 tttccactag aaactgcttc atccaataag atagggatgc ttgcagcaga tgtattccca
    14941 tatttatcca tattggtcag gaacttttct tgcggcatct ttacttttcg ggcaattttc
    15001 tcaataattc tgaggtttgc ctgatgcaaa agcagataat caactggttt gtctttcatg
    15061 acttcttgga tattgtttgc aacgtctcgt acggcaaaat caaaaatatc tctgccagtc
    15121 atttgcaaat aatacgaacc ttctgaatct tctgaataga acggattttt attttgctgg
    15181 tagccagcgg tcaatgagct acttctggta ccgtcagctg ctaatttttc tttcagcaat
    15241 tttttctgtg gattcgcttc taagagaatc ccacctgctc catcgccaaa caacacagca
    15301 gtcgtacgat cagaccaatc gattagctta gatagtgttt caccaccaat caccagtccc
    15361 ttagttgagc cgcagcggat cattttttca gccatgctta acgcataaac aaatcctgag
    15421 cacgcagcac tgatatcaaa agcgatagca tgagttgctc ccaatttccc ttgtacttgt
    15481 gctgctacag atggtgtcgt gtaatcgggc gacattgttg ctactaaaat aaaatccaaa
    15541 tctttagggt ttagattttt ctgttttatc aatttttccg ccactaaaat gcataaatca
    15601 gaagtctcct gatttgttac acatcttcgt tcacgaatac ccgtccttga cgaaatccat
    15661 tcatcagagg tatccatcat cttggaaaga tcattattag taataatctt ttcaggaagg
    15721 acgctggctg ttgcagtaat cattccaaat tgttccatta aatatcctca cttatagtct
    15781 tgcaagaagt cgtgcaagtt tttcagtgca tccagcaagg catgctgttc ctccgtagcc
    15841 attccgtcta agatgttctt gaccatttca cgatgaaaat gctgatggac ccgaaatagt
    15901 aactttcctt ttttcgttag tcccaacttt acaacgcggc ggtcatcttc actgcggata
    15961 cgttcgacat agcctttttt tactaacttg ttaatagcag tggtcaaggt cccaacagta
    16021 attgatagtt ctttggctac ttctgaagtt gtttttttct tgtacatccc gattgcctcg
    16081 atcgtgtgca tctctgtgat tgacagatct ttgaattgag attttttcag ctcagactct
    16141 tcgattgtca aaatatcatt gaagacacta acaagataat catttactgt ttttaaatct
    16201 ggttccatgt tccaccgtcc actaattagt ttgattatca aatcttttga tgtt
//
LOCUS       DAZBSA010000063        16099 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000063 DAZBSA010000000
VERSION     DAZBSA010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.063"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(226..1953)
                     /locus_tag="ACHGMT_001872"
     CDS             complement(226..1953)
                     /locus_tag="ACHGMT_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFN6835608.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(1946..3139)
                     /locus_tag="ACHGMT_001873"
     CDS             complement(1946..3139)
                     /locus_tag="ACHGMT_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFN6835609.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3326..3970)
                     /locus_tag="ACHGMT_001874"
     CDS             complement(3326..3970)
                     /locus_tag="ACHGMT_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN6835610.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4064..4198)
                     /locus_tag="ACHGMT_001875"
     CDS             complement(4064..4198)
                     /locus_tag="ACHGMT_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFN6835611.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4200..6332)
                     /gene="feoB"
                     /locus_tag="ACHGMT_001876"
     CDS             complement(4200..6332)
                     /gene="feoB"
                     /locus_tag="ACHGMT_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFN6835612.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6329..6802)
                     /locus_tag="ACHGMT_001877"
     CDS             complement(6329..6802)
                     /locus_tag="ACHGMT_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFN6835613.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7203..7427
                     /gene="nrdH"
                     /locus_tag="ACHGMT_001878"
     CDS             7203..7427
                     /gene="nrdH"
                     /locus_tag="ACHGMT_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFN6835614.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7586..9745
                     /gene="nrdE"
                     /locus_tag="ACHGMT_001879"
     CDS             7586..9745
                     /gene="nrdE"
                     /locus_tag="ACHGMT_001879"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFN6835615.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            9795..10760
                     /gene="nrdF"
                     /locus_tag="ACHGMT_001880"
     CDS             9795..10760
                     /gene="nrdF"
                     /locus_tag="ACHGMT_001880"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFN6835616.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(10849..11988)
                     /locus_tag="ACHGMT_001881"
     CDS             complement(10849..11988)
                     /locus_tag="ACHGMT_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFN6835617.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12297..13010)
                     /locus_tag="ACHGMT_001882"
     CDS             complement(12297..13010)
                     /locus_tag="ACHGMT_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFN6835618.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13264..13965)
                     /gene="nagB"
                     /locus_tag="ACHGMT_001883"
     CDS             complement(13264..13965)
                     /gene="nagB"
                     /locus_tag="ACHGMT_001883"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFN6835619.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14199..15731
                     /locus_tag="ACHGMT_001884"
     CDS             14199..15731
                     /locus_tag="ACHGMT_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFN6835620.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(15922..>16099)
                     /locus_tag="ACHGMT_001885"
     CDS             complement(15922..>16099)
                     /locus_tag="ACHGMT_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN6835621.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         4982 a         3334 c         2823 g         4960 t
ORIGIN      
        1 tcgtacataa taaactttaa tcacattagt ctttcatacc tacgatcgat ctctttgact
       61 cgcacccata aattcataat agtttgtcgg cgtctttgaa cgtttcgaag atgagctacg
      121 cattctcttt ttttatagac aaaaaagata gagacgaaca tggagcttcc aaatttggat
      181 atttgatcca attttggaag ctccatttat ttcgtgtctg tctttttatt ttttccagct
      241 ttcgactaat tcttggttct ctttgatcca gtcttttgct gcttgctgtg gatcttttcc
      301 attgttgatt tccaacataa ctttttccat atctttttct gaccaatgga aattgtctaa
      361 tactttgtaa gcttctggct ggtcttcttt caatcctttt cgaaccatcg tatggatctg
      421 ctcttcgctt cccattccgt tttcaggatc ttctaagtat ttcagatcat atttggcaaa
      481 catccaatga ggtgtccatc ctgtaactac aattggttcg tgttttttga tcgcttgacc
      541 tagtgcaacg gtcattgctc ctgaagaaga tgtctctact ttccagtctt tcaagttgtc
      601 atatttttgg atcgtgtttt cagcagccgt aactacacca gctcctggct cgattcctat
      661 gattttctta cctgcctgat cggttaactc atcgatcgaa tttacatcca tgtaggctgg
      721 cactgctaaa ccaacttttg ctcctgtcaa attggcacct aggtcttcga cttgatcttt
      781 atattgtgca tattgtgaac catgagtttt aggtagccaa gctgaaacca tggcatctga
      841 ctcgccattt gaaacagatt tccacatgat tgaattatct aatggcgtca tcgttacatc
      901 atagcccatt tgtttcaata cttcaccgac tacatttgtt gaagcaactt ctgtatccca
      961 ttcaacataa gataagttga tacgttttgt ttctttggca gatgaaaaac tccctatacc
     1021 aatcgcacct aagatcacta ctactacagc accaatgatc agtccttgac gttttttact
     1081 tttctgtgtt gttgctcctg cagctttttt attgtttggc tggttcaaat gctgtgtaaa
     1141 acggtcaatg atgattgcta agataactaa tgcaaccccg ttgacaaatc cattcccgac
     1201 ttgtgctctt tgcaatgctg ataatacgcc gcgtcctaag ccgggtgcac cgatcataga
     1261 ggcaataacg accattgaaa gtgacagcat tgttgtttga ttgatccctg ccatgatcgt
     1321 acttttagct aaaggcaatt ctagtttgaa tagtttttga cgacttgtgc tcccgaatga
     1381 atcagaagct tctactaatt ctttaggaac ttgtcgaatc cctaaattcg taaaacgaac
     1441 ggttggtggc agagcaaaga tgactgaggc aaatacccct ggaaccatcc cgataccgaa
     1501 aaatgcaact gctggaatca aataaacgaa tcctggcatt gtctgcataa agtcaaggat
     1561 cggtttgata atactttggg ctttactgct ttttgccatc aagatcccta atggtacccc
     1621 aatgatgatc gagatcacac tagataaaag aacaagtgtg acagtattca tcaaatcatt
     1681 ccatagacct tggttataaa taaaccatag accgattaat gtgaacaacg taagtcctgg
     1741 acgtttctta gaaataaaga aagctgctac cgtcaataac aagataaaca aaggagctgg
     1801 aatcactaac agtaaatttg taatccccga catcactgcc tgtccaatcg tttgtaaaaa
     1861 tgaaaatagg ccagaaaaag ttgtcgtaaa ccattctgtg atattttcta cccagtttgc
     1921 cactggtaat tgatagttca atagattatt catgttcact cacctcgctt gtttctgcta
     1981 atgcttcaat cacactacct cggatgacga caccttttaa ttttccatcc tctacaactg
     2041 caagtggagt cggtgagtca taaataatat tgaaaatatc acttaccacc atgtctttcc
     2101 caatcaccgt cacattttca tctaggaagt cgatcaatgg acgtttttgc ttcctagctt
     2161 ctaacgcttg atctgcagtg atgatccctt tcaggtgtct ctttctgtca acagccatca
     2221 gcatgctgac ttcttcatta cgcatccttg tcaatgcaac gtttggtcca tcagattcga
     2281 tattcgtcgt caatgcaggt accatgatgt tttgagccgt aagcactttg gaacgatcga
     2341 cttcttccac aaattcacgt acataatcat ttgctggatt ggtcaatatt tcttcacctg
     2401 ttccgatctg catgatttcg ccgtctttca tcaaggcgat tctgtcccca atccgtaaag
     2461 cttcattcaa atcatgggta atgaaaataa tcgttttttg aacattggct tgcaggtcca
     2521 gtaattcgtc ttgcatttct cgccggatca gcggatcaag agccgagaac gcttcgtcca
     2581 tcagcaagat ctcaggatca tttgctaaag ctctggctag gccaacccgt tgttgcatcc
     2641 caccagataa ctgactagga tattggtctt taaatgaaag caaactagaa ttttctaatg
     2701 ctttttctgc tttttcttgt cgttcttctt taggtacacc acggacttct aacccgtatt
     2761 ctgtattttc caagatcgtt cgatgtggaa acagaccgaa gttttggaaa accatattga
     2821 ttttatgacg acggacttct cgcaattctt ctttgcttaa cttcgataca tcttctccat
     2881 caatatagat actgcccgaa gttggctcaa tcagacgatt caatagacgg atcaatgtgg
     2941 atttcccact cccggaaagc cccatgatca cgaagatttc tccttctttt acgtcaaaat
     3001 tcacgtcata cacaccaacc gttgcaccgg ttttctttaa aatctccgtt ttcggctggt
     3061 gatcattcat catatctaaa gcggcttgtg tctttttacc aaatatcttt gttaaatgtt
     3121 ttacttctac ttttaccaaa caaattctcc ttttttatgt aaattcattt ttctttctga
     3181 aaaaagtgac cacactatgt taacatcatg tagtcacttt gtcaaaaatg aaagaaattt
     3241 gcttgtccaa actgaaaaaa acttggctaa atctttgtaa ataaaaggtt tagccaagtt
     3301 aatatttttt taatatttca cacaatcatt ctgcaagctg atgctccggg taaaaaaagt
     3361 ttgtcatacg ctgtagtgta tattcatttc caataaaatg aacgatgtcc cctgccgtaa
     3421 atttagcata ggggccaggt gaaacaagca gttcttcatt atgagaaatt gcgataactg
     3481 tggcgccagt cgcctgccag atattcaact cgctaattga acggtccaga tgcattgctt
     3541 tttctgataa agataattca aacggaagta aaggattcat cttatgtatt cgcttcgttt
     3601 gattgaccag ctgatccaat aattgagaaa aatgattcag ctcatcttgt tgcttctcca
     3661 cacttgccat gatttctgct tttgtatctt ggatggattg tacatcttga tattgcaata
     3721 ggaaatcttt tgctttttcc ttcgaagcaa caaatgcccc acttccatgc cgtacttcca
     3781 tgattccaag atctaccagc acattgatag ccttgcgggt agtctcagga gaagcattgt
     3841 acgtactggc aagtgttgag cgggcatgga tcttctcacc cactttatat cggttttcaa
     3901 cgatacgttc ggctaaatca acagcaatct gctggtagcg cgggcggacg atttttttct
     3961 ttccagacat tttctttcct ctttcttttt ttactttccc tcatcttact atgctctagc
     4021 aaaataagca aaaaagggga gaatagcctc ttgcaacgac tgatcatcgc ttgatctgtt
     4081 ccttttttac tgggcaagct gtttgacagt catcacaatt cttaccggat tttaaacgag
     4141 tatagacaat tgcgcctgca cttccaaaga ttaacacact taataagaat gtcgccatcc
     4201 tattcctctc cttctaatga caatatagta taaaactggt tcttggattt tggctgacgg
     4261 aacaaaacat aaacgagtcc tatcatcagt aaaatagcaa gatacgtccc taacgctacc
     4321 tgtccaccct ccaataagtg tcccaactga taaatcacaa aactaactaa ataagctaaa
     4381 ccgcattgat agcctatggc gatccaagtc catttcttgg cattcatttc ccgatggata
     4441 gccccaattg ccgcaaaaca aggcgcacaa agtaaattga aggctaacaa tgaataagct
     4501 gctgcgggag tatatgtgtg ttgtagcact tgccagattt ctctgccatt ttcggatact
     4561 tccgcatgac caaacaaaat accaaatgtg ccaataatat tttctttagc gaccaatcct
     4621 gtaatcgctg cgactgttcc ttgccagttt ccccaaccaa gcggctgaaa aacaactgcc
     4681 aggattcgcc cgaaaaaggc taagatactt tggtcttcag aaacaggttg agcataaaaa
     4741 ttataagaag agctgaacca gatcacgata ctagaaacaa agataatcgt tcctgctttt
     4801 ttaacaaaag acttgctgcg gtcataggtt tgtcttagga ccgctgacca ttgaggaaaa
     4861 tgatatgcgg gcaattccat gataaaagga gctgtatctc cagcaaacaa acgtgttttt
     4921 ttcaagctga ttccagagaa aataattgca gtcataccaa gaaaataggc agaaggagct
     4981 acccaagatt gatgaggaaa aaaggcccct gctactaagg caataatcgg caactttgca
     5041 gaacaaggca tgaaggttgt gaccatgatc gtcaaacgac gatcttgctc gttttcaatc
     5101 gtgcgactgg ccatgacgcc tggcactcca catcctgtag caatcagcat aggaatgaag
     5161 gattttcctg aaagaccgaa tttcctgaaa agtcgatcca tgacaaaagc gatcctcgcc
     5221 atatatccgc agtcttccag aaagctcaag cataaaaaca atataatcaa ttgcggtaga
     5281 aatcctaaca ctgctcccac gcctgcgatg atgccgttca agatcagctc ctgcatccat
     5341 cccgctactt gccattcagc taacaatctg ccaacatgtt ctggtaacca tgtaccaaac
     5401 aatgtgtcat tgatccaatc tgtccccatt gttcctactg tttgaatagc cagataatag
     5461 atcagccaca tcacaaatgc gaagatcggc aaggctaacc aacgattcgt tgcaatctga
     5521 tcgattttgt cactcatcga taattggaaa gaatcatttg acacaacaca gagtgtacat
     5581 agttgggtaa taaattcata acgttcattg accagaatcg tctcactatc gtcgtccaat
     5641 agtttctctg ttaactggat cagttgctcg atctcttttt gctgagaaac tgctatctcg
     5701 taatcctctc ttaccttctg atctcgttca aaatatttca ttgcatacca gcgtttcttg
     5761 ccgctttgtt ctcctagtac atcttcaatt tcatgtaaag ctgcttctaa acgtggatca
     5821 tatgtacagg gaacaggaat agaaggccgc ttttttgcag actcctgaat cttttcgatt
     5881 agtttttcga ttccttgctt tttcaatacg ctgattggta aaactggtac accaagacca
     5941 taggaaagct tttctagatt gatttttttt ccttcttttt tgatgacatc catcatattc
     6001 agaccaatga tcataggaat gtctaaatcc aataactgcg tagttaaata aagatttcgt
     6061 tctaaattgg tagcatcaat aatgttgatg atcagttcag gctgttcctg aaccaaataa
     6121 tctcttgcaa tcacttcttc tggtgtataa ggagacaaag aatagatgcc gggtaggtcc
     6181 tgaatatgaa tgttcggatt ttttttcaac gatccttctt ttttttctac tgttacgccc
     6241 ggccaattcc ctacatattg attcgaacca gtcaagagat tgaacaagct agttttcccg
     6301 ctgtttggat ttccgactaa cgcgatattc attctttttc ctccctttgt atcaagatta
     6361 gttcggcctc tgactttcgt aacgtcagtt cataccctcg cactgtcagt tcgattggat
     6421 ctcccagagg ggctagtttt cggataacta tttttacatt tcgcgtcaat cccatatcca
     6481 ttaaccgtcg cttcactgcg ccttgtccgt ggattgctac gacgatccca gtctctcctg
     6541 cttttaattg atcaagagaa gtccagcttt cagaggaagc agtcttttta ttgacaaaga
     6601 tttgacctag tatttcccga cttactgcca ctcgatgact ttttaccagt aaaatggcat
     6661 tgttgcctga taaattgacg acgacgattt tacttccggc aacgatcccg atattttgca
     6721 aatggcgttt tgttttttct tctgtttcga ttcgttcgac ttgataaacg aaattctgct
     6781 ttgcctgcgc taaggtctgc atattgattc ctctcttgtg aaataaatca tgtattaaaa
     6841 tcattcatta tatgttctaa ttttcttacg tgttacgcaa ataaatgaga atcattctca
     6901 catagaatac tctcggtaat tgagaatgtc aattatactt gtgatattat gagcgaatct
     6961 ttagtattga tagataaaaa tgagaaaaaa atatttcttc atagttttcc acaatttttt
     7021 ctctattaaa tttgctattt tcccacacga tacttgtttt cagctctttt ttcaaaaaca
     7081 caaaacttag tattccacag tttccacaac acaatatgta gtgtttgttg tttgactttt
     7141 ttactaagag ttttatacta tctattatag ataataagaa tgtgttagag gaggcactca
     7201 ccatgaacgt aaaaatcttt tctaaaaata attgtatgca atgtaagatg gtcaaacggt
     7261 ttttaacaga aaatcaaatt gcttttgaag aagtcaatat cgatgaacag cctgaagcag
     7321 tagactggtt gaaagcacaa ggattccaaa gtgttcctgt cattacttct gatgcagaaa
     7381 cagttatcgg attccgtcct gatcagttgc gtaaacttgc cagctaacat gactaaaaga
     7441 atcaaaacgt gatacatgga tttgaagggc ttgagacagc accagttttt gtcgcaaact
     7501 tccaaatcct tttttaaagc ttaaaaacaa tgcaatgatt tgcaatcaag catcatcaaa
     7561 tacgaaatga aggttgggcg tacgtatgag tcttaaaaat ttacaagatg tcagttattt
     7621 caagctgaat aatgaaatca atcggccagt caatggtcaa atccctttga ataaggataa
     7681 agaagcttta gttgcctttt ttgaggaaaa tgtcaaacca aatacgatga cttttccatc
     7741 tattatggaa aaaatcaatt acttgatcga tcaagaatat atagaagcag aattcattca
     7801 attgtattca ccatcattta ttgaaaagct atatcaattt ttggacgagc aacatttttc
     7861 tttcaaatct tttatggctg cttacaaatt ttattctcaa tatgcgttga aaaccaacga
     7921 cggttccgct tatctggaaa cttatgaaga tcgagtagcc ttcaatgcgc tttactttgc
     7981 gaacggggat gaagaattag cacttgctct tgcagacgaa atgatccatc aacgatatca
     8041 gccagctaca ccttctttct tgaatgctgg acgtaaacgt cggggcgaat tggtgtcttg
     8101 tttcttgact caagtcactg acgatatgaa ctctatcggg cgctcaatca actctgcttt
     8161 gcaattgtcc cgtattggtg gcggcgtagg gattacttta tcaaatcttc gtgaagccgg
     8221 cgctcctatc aaaggatatg agggtgctgc aagtggtgtc gttccagtca tgaaattatt
     8281 cgaagatagt ttcagctatt ctaatcaatt aggtcagcgt caaggagctg gcgtggttta
     8341 tttgaacgtt ttccatccag acattattgc tttcctttca gcaaaaaaag aaaatgcaga
     8401 tgaaaagatt cgcgtaaaaa ctctttctct tggcgtgatc gtacctgata aattttatga
     8461 attgacacgt aaaaacgaag atatgtattt atttagtcca tatagtgtcg aaaaagaata
     8521 tggtgtccca ttctcttatg taaatattac agctgagtat gatcatctag ttgcaaatcc
     8581 aaacattcgc aaacaaaaaa tcaaagcgcg tgatcttgaa aatgaaatct caaaattaca
     8641 acaagaatct ggctatcctt acgtaatcaa tattgataca gcgaaccgcg agaatccaat
     8701 cgatggcaag atcatcatga gtaatttatg ttcagagatt ttacaagttc aaacgccttc
     8761 tgtgattaac ggcaaacagg aatatgaagt attaggaaca gatatctcat gtaacttagg
     8821 ctcgacaaat atcgtgaatt tgatgcaaag ccctaacttt ggtcaatctg tgcgtacgat
     8881 gactcgtgca ttgacattta ttacagatgc atctgatatc gatgtcgtac caccgatcca
     8941 aaacggaaat aagctgaatc atacgattgg attaggtgcc atgggcttac acacttatct
     9001 tgcaaaagaa caaatggaat atggctcaga ggattcatta gactttacaa atatttactt
     9061 catgctgttg aattattgga cacttgttga aagcaacaat atcgctcgtg aaagaaaaca
     9121 agttttccat aattttgaaa aatcagctta tgctgacggc acttatttcg aaaaatacgt
     9181 aactggagaa ttccagccaa aatcagaaaa agtcaaagca ttgtttgaag gcatttttat
     9241 cccaactgct gaagattgga atgctttgaa acaagcagta atgaaagatg gactgtatca
     9301 tcaaaatcgc ttggcagtag caccaaatgg ctcaatttca tatatcaacg atacgagtgc
     9361 aagtattcat cctatcactc gcatgatcga agaacgccaa gagaagaaaa tcggtaaaat
     9421 ttattaccca gcgccttatc tatccaatga gacgattccg tattacactt ctgcttatga
     9481 tatggacatg cgtaaagtaa tcgatgttta tgcaacggca caacaacacg tcgatcaagg
     9541 catgagtttg actttattca tgcgttctga aatcccagaa ggcatttatg aatggaagac
     9601 aacaacgaag caaacgacac gtgatttgaa tattttaaga cattacgctt tccacaaagg
     9661 aatcaaatcc atttattacg tacgtacctt tactgatgat gcagaagaaa tcggaagcaa
     9721 tcaatgtgaa agctgcgtga tctaatatca tagatagatt cgcttattca tattttaatt
     9781 aaaggagcat tctcatgtca gaaacatatt atgaagcaat aaactggaat gaaattgaag
     9841 atatcattga taaatccact tgggagaagc tgacagagca attttggcta gatacacgta
     9901 tcccactttc aaatgaccta gatgattggc gtacattgtc tcaattggaa aaagatacag
     9961 taggacatgt atttggcggt ttgacactgc tagatactgt ccaatctgaa agtggcatgg
    10021 atcagttgcg taaagatgtc cgcactcccc atgaagaagc cgttttgaac aatattcaat
    10081 tcatggaatc cgttcatgcc aaaagttatt cttctatttt tagtacactg aatacgaaaa
    10141 aagaaatcga agaaattttt gactggacaa atacgaatcc ttatctgcaa aaaaaagctg
    10201 agcgtatcaa tgaaatctac aaacacggta caccgctaga aaagaaaatt gccagcgtct
    10261 ttttagaaac attcttgttc tattcaggtt tttatactcc tctttattat ttaggaaaca
    10321 acaaattggc taacgtagca gagatcatca aactaatcat ccgagatgaa tctgtacatg
    10381 gaacctacat cggttacaaa ttccaattag gattcaacga gttacctgaa gcagaacaag
    10441 aaacgttaaa agactggatg tacaatcttc tttatgagct ttacgaaaac gaagaacgtt
    10501 atacagaaga attatacgat ccaatcggct ggacagaaga agtcaagaca ttcctacgct
    10561 ataatgccaa caaagcattg atgaacctag ggatggatcc actctttcca gatactgcca
    10621 atgatgtcaa cccaatcgta atgaacggga tttcaacagg cacaagcaac catgacttct
    10681 tctcacaagt cggaaacggc tatttgttag gtcatgtaga agcgatgaaa gacgacgatt
    10741 atttgattgg attggaataa ggatttattt taattcaaaa atttttcgaa aaataaaaag
    10801 agtttagcgt acagatattt taaatgtaca ctgaactctt ttttatgctt attcacttgt
    10861 ttcttccagc acttttggac tgtctaactt ttccgaaaca tgttccattc ttctcaataa
    10921 atagattttc cggatattta atattatcgt cttaatcact gcatagacag gtatgcaaat
    10981 cagcatgcct aatattcctg cgacacttcc tgctccaatc aataccaaga tgatcgtcaa
    11041 aggatggata ttcattgatt tcccaaataa caacggcttg atcaaatttc catctaattg
    11101 ttggatcact aaaatcgaca acgccatata caaagcttga aatggagaga taaacaaacc
    11161 gacaatgatt gctggtaccg ctccaataaa agggccgaca tacgggataa tattcgtgat
    11221 tccgcaaatg accgcaagca acaagctata aggctgatcg aatattctca ttgccaaaaa
    11281 gctcattaca ccaataatca aagcatctaa gatcgtactg ctgatatagg cagataatgt
    11341 ttcgtttaat tcttttaccg tttggcgtaa ctctttacga atcgctttcg gaaagaagtg
    11401 agacaacgca tcaataaatt tgtgcccatc cttgaacata aatagcaaaa taaatggaac
    11461 ggtaaataac aaaataaaaa attgcatcat aaatgaaatg atttttgaga cacttgcaga
    11521 taaactgact aatatgatat tgaacaaatt actaagagat aaatttgctg atgctaattg
    11581 ttcttccaaa ttgaaatttt taaactcttc ccgctgcgcc aattcattta accatttgct
    11641 tgtttcatct gcatacattg gcaagctctg ggtcaattga attgtctgtt ccattaactg
    11701 aggcactaca ttcatgacag ttaaaacaac taaaatgatt acgaccaaaa acgtcaataa
    11761 aaaaccgaac actctcggga tccgatgctt ttcgagaaaa acaacgattg gatcgaacat
    11821 ataatagata aaaccagcaa caagcaatgg taaaaaaatt gccgaaagca ttttcaatat
    11881 tggactaaaa atattcggca tttgtaaaat aaagtacacg ccgatgatcg tgagtactag
    11941 ctccactgtc cagaaaaata attttgattg tttaaaacgt gattgcatac atctcctcca
    12001 aggtcctttg atacttctat tctatcattt aacaaagagt tacgcgctga gattttcatt
    12061 tgcttaaaaa aaacaaaaaa aataaacata ttttaatgag tagacgagta cttaaacaaa
    12121 aacacggttt ggagtagtga caaaaattat actgcagtcc ctttttccaa ataaacggtg
    12181 ttcaaaagct tgcatcttcg taataagccc agccaaacga aaaatataag gaactatttt
    12241 tatttggctg ggcttattac ttgatgtagg acatctattt tacaacctca gatcatttat
    12301 tgcgcaatac tgactaaatg caactttgtg atatttttgt gtacggacaa ctcctcgatc
    12361 acatcggcat aagtgagccc ttttggaaga tcaatcgtat agatattttt gtacactcgg
    12421 tagtcatcaa cgaattttac gtcaaaattc acatcttcga ttacgatatt tttctcttca
    12481 aaataatcgg taagaaaatc tttggtttct tttctatgga taaattggat ttctaacttt
    12541 ttagtcgtag gtacatggat cacgcgtttc actactgtga gtgcaatagc aatcccgata
    12601 aaactgatga tcgcaatgga atagtaaccc atgccgatgg cgatccctaa tccagcaatt
    12661 gcccataaag aagcggcagt cgtaagtccc gtcaccgatt gtttcgtgac gatgatcgtt
    12721 cccgcgccta agaaaccgac accactcacc acttgagcaa tcagccttgc ttcatctgaa
    12781 cgaatcgttc cagcaagctg tggatactct aaagcatctc ttaatgcacc actagcgatc
    12841 tcaacttgga tcaatgcaat gatacatgct cccatacaaa ctaaaatatg cgtacgcata
    12901 cctgctggac gatttttata ttgtcgttca aaaccgattg ctccgccaaa tagcatagcg
    12961 atccctaaac gtaaaataat ctctggaatt gtcaatacca tatcattcat ctaaacaccc
    13021 tccagtccaa tatatcatgt ctgaaaggag cagacaaaaa aagtgcaatt tttacctctt
    13081 acgaaaaaag gactgtgaca aaatttttgt catagtcctt tttatgaata aacggtgttc
    13141 aatcagctga ccctcggtat tactcaacaa acggcaaaat atggggagct atttttcctt
    13201 tgtcttctct aatactcagg tcaaaacgct gatttcacaa cctctttagc ctactattga
    13261 aaattacaac ttacttgctg cttcttcatc gatgatcaca acaacattgt cgtgattttg
    13321 caatacactt gctggcaaat gattcgttac aggtccttca atcatccctt tgattgcatc
    13381 agctttgctt tcgccaaatg ccatcaagac gatttcttta cttttcataa ttgaaccgat
    13441 tcccattgaa acagcttttg ttggtacatc ttctgcttta tcgaagaatc gtttgtttgc
    13501 attgattgtt gattctgtta agtcaactac tgaagtcgtt ccgtcaaatg aagcaccagg
    13561 ttcattgaaa ccgatatgtc cattttgccc gattcctaag atttggatat cgattggatg
    13621 tgcatcgatg atttgatcat actttttgca tgctgcttcc agatcttctg cttttccatc
    13681 tggtacaaac gtttctttga atggtttttt atcaaataat tggacatcca tgaaatgacg
    13741 gtagctttgt tcatctgaac cgcctagtcc aacgtactca tctaagttta cagaagtcat
    13801 ttcactgaaa tccaaatcgc ttgaagtcat ttctttatat aaagtaactg gtgtgcttcc
    13861 tgttgccaat cctaatactt ttgcaccttt gttcatacct tctttgatga tttcaaatgc
    13921 tttttttccg ccttcttcag cgttttttac acgaatgatt tccatagtga ttgctccttt
    13981 atattttggt atagcccaat tatagcattc tttttttctt tttacaataa ttagtataga
    14041 ccaaattgca tttttttcat tttcttgaca ataaatagga atccgtgtta ggtttcataa
    14101 gatggttcaa tcaaacaatc catgttgcat gaaaaccaag caagtgattc actgtggacg
    14161 ctttatgtct gcagtttttt tatgaaaaag gagttattat gagtatactg acattaaaca
    14221 acattaccca gcaatttggc gacaaaatcc tctatgaagg tgtcgatctg caattaaatg
    14281 ctggcgaaca tcttgggctg attggacaaa acggagccgg taaaagtaca ttgattaaaa
    14341 tcatcactgg tgagatctta ccagatgacg gacagatcca ttggcaaaaa aatattcaca
    14401 aaggttacct cgatcaatat gtggaagtcg atgaaacatt aacaatcgaa gaatttctaa
    14461 aaactgcgta cgcaaaagaa tacgaaaagg aagaaaaaat tgcttttctt taccaaacat
    14521 atagcgagac cttcgatgat tccttgctgg aaaaagctgg gaaactccaa acggaattag
    14581 accaaggagt gttttatcaa atcgacacct tagttgctga gatgagcagc gggttgggga
    14641 tcgatgtctt aggtttagat tcactgttga aaaatctaag tggtggacaa cgttcaaagg
    14701 tgattttagc aaaactactt ttagaaaacc cagatgttct gatcttagat gaaccaacca
    14761 atcacttaga tgatcagcat atccaatggc tgacgcagtt tcttcaggat tttgacggtg
    14821 cgtatatcgt gatttcccat gaccaagaat ttcttgatca gatcaccacc catatcgcag
    14881 acatcgagtt tggaaagatt actaagtata ctggccatct aaaacaagca ttgaaacaaa
    14941 aggaacaaaa tcgcgaaagc tatttgcgcc aatatcatgc tcaacaaaaa caaatcgaaa
    15001 agaccgaagc ttatattcga aaatacaaag ccggttcccg atcaacgatg gcaaaaagcc
    15061 gacaaaaaca attagataaa atcgaacggc tgacgcctcc agcttcagca agtaaagctg
    15121 tctttgattt tccattctcg cctattgtca cgacattagc agtggaaaca actgatttag
    15181 tcatcggata tgatcgaccg ctacttgcac ccatcaattt gaccatccgc tttggtgaaa
    15241 atattgccat tcgaggcttc aacgggattg gaaaatctac tttattaaaa acactgatcg
    15301 gtgagattga aaaaataagt ggcgatttcc acttcccaga taacacaaaa atcaattact
    15361 tttcacaaga tctgtactgg gaaaatccat tagaaacacc actacaatat ttaagcagtc
    15421 aatttccaaa agcgactatc aaagaattac gtcgccaact agctaaagca gggttagtta
    15481 atcaattagc aagtgaacct ctcactacat taagtggtgg ggaacaaact aaggtgaaac
    15541 ttgcacagtt gacaatgaac caaggtaatt tactgattct cgatgaaccg acgaaccata
    15601 tcgatcaaga aacgaaagaa agcttacaag acgggataca gaaatttcca ggaacagtaa
    15661 tcatcgtctc tcatgaacaa gaattttatc aagatcttgt gcaacgagtc attgagattg
    15721 aaccaaaata aacgtttgat tgaatagttg aaaaaaagga gctgtgacaa aagttttgtc
    15781 acaactcctt cttctgaata aatggtggcc aattagctga ccttcggtat tagctcaaaa
    15841 aacggcaaaa tatgaggagc tatttttatt tggctattct tattacttga tgcagaacag
    15901 cttttcacaa cctcttttcg tctatttttt attttcgaga tttctctttc ctttaaaaac
    15961 aactgtttga aagaagaacg cacatccttg aaaaacaagc ccgctaaccg ttacacctaa
    16021 ccagccatac tgctgccaca ttgtcgtccc tagcaatgaa ccagccgcac ctccaataaa
    16081 gtacataaac ataaataca
//
LOCUS       DAZBSA010000064        15774 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000064 DAZBSA010000000
VERSION     DAZBSA010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.064"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            123..1550
                     /locus_tag="ACHGMT_001886"
     CDS             123..1550
                     /locus_tag="ACHGMT_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6835622.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1642..2007)
                     /locus_tag="ACHGMT_001887"
     CDS             complement(1642..2007)
                     /locus_tag="ACHGMT_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFN6835623.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(2004..2369)
                     /locus_tag="ACHGMT_001888"
     CDS             complement(2004..2369)
                     /locus_tag="ACHGMT_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFN6835624.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2561..2809)
                     /locus_tag="ACHGMT_001889"
     CDS             complement(2561..2809)
                     /locus_tag="ACHGMT_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835625.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2808..2936
                     /locus_tag="ACHGMT_001890"
     CDS             2808..2936
                     /locus_tag="ACHGMT_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFN6835626.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2993..4687
                     /locus_tag="ACHGMT_001891"
     CDS             2993..4687
                     /locus_tag="ACHGMT_001891"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFN6835627.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4787..4960
                     /locus_tag="ACHGMT_001892"
     CDS             4787..4960
                     /locus_tag="ACHGMT_001892"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835628.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5338..6471
                     /locus_tag="ACHGMT_001893"
     CDS             5338..6471
                     /locus_tag="ACHGMT_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFN6835629.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6502..6822
                     /locus_tag="ACHGMT_001894"
     CDS             6502..6822
                     /locus_tag="ACHGMT_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFN6835630.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6851..7315)
                     /locus_tag="ACHGMT_001895"
     CDS             complement(6851..7315)
                     /locus_tag="ACHGMT_001895"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6835631.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7447..8700)
                     /locus_tag="ACHGMT_001896"
     CDS             complement(7447..8700)
                     /locus_tag="ACHGMT_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFN6835632.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8729..9046)
                     /locus_tag="ACHGMT_001897"
     CDS             complement(8729..9046)
                     /locus_tag="ACHGMT_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFN6835633.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9072..9392)
                     /locus_tag="ACHGMT_001898"
     CDS             complement(9072..9392)
                     /locus_tag="ACHGMT_001898"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN6835634.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9856..11271)
                     /locus_tag="ACHGMT_001899"
     CDS             complement(9856..11271)
                     /locus_tag="ACHGMT_001899"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN6835635.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11288..12604)
                     /locus_tag="ACHGMT_001900"
     CDS             complement(11288..12604)
                     /locus_tag="ACHGMT_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN6835636.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12754..13452)
                     /locus_tag="ACHGMT_001901"
     CDS             complement(12754..13452)
                     /locus_tag="ACHGMT_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFN6835637.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13741..14730)
                     /locus_tag="ACHGMT_001902"
     CDS             complement(13741..14730)
                     /locus_tag="ACHGMT_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFN6835638.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14810..15643)
                     /gene="msrA"
                     /locus_tag="ACHGMT_001903"
     CDS             complement(14810..15643)
                     /gene="msrA"
                     /locus_tag="ACHGMT_001903"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFN6835639.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
BASE COUNT         4911 a         3195 c         2698 g         4970 t
ORIGIN      
        1 ataaaaaacc ttttttaacg gcatttatat gctttttcag aaattaactg atgatttcaa
       61 aaagaaaaca tgtcataata tttcccatat tgtgcaaaaa aatacaaggc ggaggctcta
      121 ctatgattga agaatatatt gagaaaaata ttttaagaca gctgttttta tgtgggcaat
      181 tctatgtaaa taaagaagtc aatctagaaa agctctccaa tctcctgcat gtttgcaaaa
      241 ccactttact caatgatatc aataacataa aaaaagaatt tgaagaacag atcgcttata
      301 cacatcggga aaaggaccgt tacactttat atttttcaga acatatacct cgatgcaaga
      361 tcatgcagca attgtctcaa aattcgttgt ttttaaaaac ttgtctgctt tatttggaag
      421 aagatgaacc tgattatctt caattaactg aatgtgaatt catctctgtc agcaaagctt
      481 attcattaaa aaaacaagta ttagcttatt ttaatgactg cgggatcgaa atcgaccgct
      541 actcccctcg attcactgaa atggaaagac gtctgctgct tcttaatgtg tcgtatcgtt
      601 taggcggttt taattcttgg gagctacctg aatcgttttt tgaacgtgcc gatcgtttca
      661 ttgaatccgt aacggaaaat agtggcagat tttatgataa agaaaataaa gaaatcttat
      721 cgattggttt tgctatcagt ttcctacgcc agcaagtgtg tgctgtgaca atagattcaa
      781 aatttatcga agaaatcaaa aaacgtccta tttacaatta tgtcgaatca gcatgggaaa
      841 acactgattt ccaaacctat tataaaaaag aagaattcgc ctttattttg acactattca
      901 atctttgtaa ttatggtttc cattcttatc agttgattgc ggaagacttt caacagctac
      961 accaagtgtt tattgacaat actcccgaaa taaaagaact agttgctacc tttgaatctc
     1021 acttcaatca agaattattc ggaaaccaac cgttcgaacg tgcgctgatt catctgatgc
     1081 gttcggcttg ggataactat caattattca tgccagaaaa attttatctt ttaaatgagg
     1141 aacagacaaa tttatataaa gaagtccaga caattttttc cagctggtcc agccagttac
     1201 cttatgatct acggttgaat cctaattgca tgcgggcttt tgtgatcgaa ttgagcggaa
     1261 ttctccgtct gacaaaagaa catctgacta tctatatcgt cacaaattcg gatgttcatt
     1321 atctgattta tcgggaagcc ttggaagctg taactacttt cgattttcaa gttgcaccaa
     1381 caatttattc aagtatatct gatataaaga agtatgcgca gcagtcctca aaccgggtat
     1441 tatgtgaacg tacattatat acaccagatg cggtccaata tgaaaatatt attcctattt
     1501 cgatcaatac gatcgatcga gctatcattt cagctgtgca aaacaaataa ggggctgtga
     1561 caaaaatcat gtcacagcct cttatttgtt gaagatgccc atttctgtaa caatctcttg
     1621 atacacgtca ggctcacaac ctcacttatt ttggacatct acgggtccga tctctttatt
     1681 taaaaaataa gaaatgatgg ttcggataac taccaacgcc gctagttgga aaatatcctg
     1741 caaggtaggt ttgataatcg attcgatgat cccagctgaa acaagaattt ccaaacttag
     1801 aagtatatag cctcccaaca tctttttaat agcattgttc tcgctgactc ggtcgctttg
     1861 cacttttgta tggacaaata aatatttcaa aaaatccttg attgatagac aaacacccca
     1921 aaccaataaa cagatagcaa agatatcaag tcccagtaca agtttttcga aatatggaag
     1981 aagaaactgc atcaactctg tcgtcatcct acctctcctc ttctttgttt ttctttgtct
     2041 tctatttcct gattcaagaa ataagaaatg actgttcgaa tgataataat aagtgcaagc
     2101 ttcaaaatat ctgtccaagt cggcttgata actgactcga tgatatcagc aacgactagt
     2161 acttccaagc ttaacaaaat ataacttgct agcagttttc ttgtatctgc attacgagta
     2221 ttacgttctg catagttttt cttgacatga gaccaatcaa aaagtttgaa tagaacaaga
     2281 acgatcccga caattaagat accgatagaa caaatattta aaatagcaat gatccattct
     2341 agaaattcaa ttaaatttga gcgaaacata tacgctacct actttctttt aatttgttcc
     2401 tattctttta ttgtaaaaga aagttacctt gttcgtctat ttaaaagttg ttctaatgat
     2461 aacggttcgc ttaaattttt ctttttcact ctcccatttt tctccctcca tccataaatc
     2521 gtggaagtat ccaaaacaac gtagcaagtt aaaataaggc ttagtccgtc atcagctttt
     2581 caaagaagca ctgtctcggt gctttttttg tacccaaatt tctaaaaata gaaccataca
     2641 cctcctcaaa tcttttcttc ttacctaccc tatttttatt tacaggaaaa tcagacactt
     2701 cttctttttt gtcaaaattt cctttttctt ctcgtcctat catttggcat tctaataaaa
     2761 acgaactatc cttggatata gaaagagaaa acaaggaggc gtttttcatg agtaaaaaat
     2821 taatcaaagt cggcatcggt ttaggtttgc ttgcccttgg cgctgcatat ctaggaaaaa
     2881 agacaggact attcgaagat gacagtcatt tatatgatga attcgaatca atctagcatc
     2941 ctatttagtt gaatcactac ggacatactt ggaagacatt ggaggaatca caatgagaaa
     3001 aagtaaagca ataatgattg gtgccggtat tgccaatatg gcagcggctg tttatttgat
     3061 tcaagaaggc aaatggaaag gggatcagat caccttttat tcattggatg atcacggttc
     3121 aaatgatggt gcaccgaccg acactgtagt ggatgaatat tggaataaaa accatccatt
     3181 agaaaaccaa aaaggctata ttgctcgagg cggtcgcatg cttaattacc gtacctatgt
     3241 agatttgatg gatctgttaa gtcgaatacc ttctgttact gagccaggga tgactgctga
     3301 agaagatacg agagattttg atgcaaaaca ccgaaccttt gacaaagcac gtttgatgga
     3361 aggtggcaaa ggaatcattg atggcggaaa actgggattc aataataaag accgtattct
     3421 tctgactaaa ctgattgcta tgcctgattc agaagaagaa aaactagaca atatcactat
     3481 cgaagaatat ttcaaagatg atccacactt ttttgaaaca aacttctggt ttatgtggga
     3541 aacaacattt gctttcagaa cacgtagttc tgctcaagaa ttgcgtcgct atatgcatca
     3601 aatgatttat gaattcactc aaatcgaaca tctagtgggc gtcaatcgaa cacgttacaa
     3661 tcaatacgaa agtattatgc ttcctttgat cgagtacttg aaagaacaag gatgcaaaat
     3721 cattttgaac cgccgtgtca ctgactggga attcaaagaa acaccgatgc aagatgaaat
     3781 cactgtgact ggtttgaaaa ttaccaatac actgactcaa gaagaagaat acgttgtggt
     3841 cgatgaagat acagcagtta ttttcactaa tgggtccatt acagattcag ctactttagg
     3901 agattacgaa acaccagctg tagaaaatat ggattatggt gcagcttcta gtctctggaa
     3961 aaaagccagt gaacgtttct acaacttggg aaatcctgac aaattctttg ctgatcgaga
     4021 tgcaagcgaa tgggtcagct ttaccctgac gacgaaaaac catttgttgt tgaatgaaat
     4081 cacccgtatc acgactcaag aaccaggaaa tgccttgaat tcatttattt caaccacacc
     4141 gatcacgccg ttaggccaaa aagatgtcaa tatgtctatt gtcgtgcatc atcaaccgca
     4201 ttttacgtct cagaaaccaa atgaaacagt tatttggggt tatttccttt atccaagacg
     4261 acgtggcgaa tttgtggata aacagtatat taaaatgaat ggaaaagaaa tgctagaaga
     4321 actgattgga caactttcaa aagtagatcc tggaccagtg aatattcgtg aaaaagaaac
     4381 agaaattttc gacagcgtaa taaataatat ccctgtctat atgccttatg cttccgcgtt
     4441 gttcaataat cgtgccaaaa gcgatcgacc aaaagtcatt cctaaacatt cgaccaatct
     4501 ggcctttaca ggagaatttg tcgagcagcc ttatcagatg atctttactg aacaaagtgc
     4561 tgtacgttct ggagaaatcg ctgctttcca ttttgcgggt attcctatgt cccgtcttgt
     4621 caaaacaccg cgttacgaca aagatattcc tactctagct cgtgccgcaa aaaaaatgtt
     4681 tgaataatca agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa caaccaggct
     4741 aaaacagctt cttatgtttt tgatttggtt ggacttatta ccgcagatgc aagcttttga
     4801 acaccgttta ccaagaggtt atgaaaaagc tgccctgcat caagtaataa gagcagccaa
     4861 gctaaaacag cttcttatgt ttttgacttg gttagactta ttaccgcaga tgcaagcttt
     4921 tgaacaccgt ttaccaagag gttatgaaaa aggcgtttag tttcgagtat gaagaaaaat
     4981 tttgtgaaaa tggttcctca cgtttttgca taaatttgac ttaataccga gaaactgatc
     5041 ccttctgtca taacctcttt ttatttatgt ctgtttcttt ttgaaaacaa ctattttttc
     5101 aacgtttact ctcttgccaa tccaacatca tctaacggat acaactattt caatgattac
     5161 cattacacta aaaaatcgga aacacacctc tccgaacaca aatacataag actatagaat
     5221 gtttcattta agaaaaaaag cgtttcatta tcaaaaaggt aagatctcct ttaaaatgaa
     5281 attatctgta gtactaataa tgcagacaat tttatgaatc tgaaaggagc atctccaatg
     5341 aaagcagtca cttggcaagg aaaacagaaa atggaaatca gaaacgtaga tgatccaaaa
     5401 atattagaac caacagatgc aatcatccgt atcacagcaa cagccatttg cggctccgat
     5461 ctgcatcttt accatcatgg tgaagcagtt ttggaaaaag ggtatgttgt aggacacgaa
     5521 ccaatgggaa tcgttgaaga agtgggatct caagttcaaa cattgaaaaa agggcaacgt
     5581 gtggttatcc cttttaatat tagttgtggt cattgtcatt tctgcctgaa tcacatggaa
     5641 agccaatgtg atgaatcaaa tccagatcag ttatatggcg ggcttttcgg atttggcaag
     5701 ttgaacggta atcattgggg tggtcaagcg gaatatctaa gagttcctta tgcagactct
     5761 acttcatttg ttgtaccaga taatgatctt cctgatgaaa aagttttatt tctatcagat
     5821 attttgccca cagcttattg gagcgttgaa aacgcaggag taaaaaaagg cgatacagtt
     5881 gtgattctag gttctggtcc agtcggtcta ttcgctcaaa aattcgctgt tatggctgga
     5941 gcagagcgcg taatcgcagt agatccagta cagcaccgtc tagataaagc ggctaattac
     6001 aatggggttg aaacttattt cttagaagac acggcacaag ctggaacaga attgtatgaa
     6061 ctggctaaag gtggcgcaga tgtcattatc gattgtgtag gaatggacgg attagagcct
     6121 gtcaaagaaa aagcgaaaaa tttagtaagc ttacaaagcg gcacaatctc tccattacag
     6181 atggcatcgc aagctgtcaa aaaatttgga acagtccaag taactggtgt ttatatgact
     6241 ccagcttctt cttacccgct gcaagaattt tttatgcggg atatcgctgt caagcatgga
     6301 caggcaccaa ttatccatct aatgccaaaa atttacgcaa tgattgcaga aggactattc
     6361 gatcctacac aaatcattac tcattcgctg ccactatcag aagcagcaca agcatatgag
     6421 atctttgaca aaaaagaaga taaaaatata aaagttgttt tgaaaccttg aattcagtaa
     6481 aaaatacagg tgattttctt tatgcccaaa atcaaacgaa atacccatga gatcgaaata
     6541 ggaaaagaag aagatatcga aatacgagga cgtcgcttcc gcttctattt ccaaaatcgt
     6601 tacacattga tctcactggc tgtcgattta ttaaccggga ttttctatat ttttggaagc
     6661 cttgcttcct taactaaaat ccccgatcga tatggcacct atttttattt ggctggtgct
     6721 attttcttga cgattcgtcc tattctcaga atcgtaagga acatttttat ttacgacgaa
     6781 aaaaaagcaa tgaagagaac aagcaaaaaa aaagaggcat gagaattttt caaatctcat
     6841 gcctcttttt tcaccctatc gtatcacttt gatacagacg agcggtcgtc ccactttctt
     6901 ccttgaccat tgtcaaaaat gtccactcgt atcgttcata aaacgtggta tgatctgtaa
     6961 ttaagtaaag tttttcgtac ccaagttgat gcatttcttt tctgatttcc gctaatatcc
     7021 gcttggctat cccctgctta cgcttttccg gttcaacaaa cagggcacaa agatttggtg
     7081 tcagatcagg gcggttatgg aagtcgtttt caatgacgcc cgctcctccg acgatggttt
     7141 ctttttcatc taagaccaca taccattgag gaatactttc cttattagcg attccagtac
     7201 gaatactttc ctcatacaca acagcaggaa ctccccattt tgatgaaaac caattcgcgc
     7261 tttgttttac taattctggg tgttctctta atttgatcca gttatatgta accaatgttt
     7321 aaacctccga ctaaaataaa acgtttacaa aaattataca agtctttatg aaaattagca
     7381 atgacaacaa tagtgatgta aaaaaaaaaa cggaaaacaa tcacttgttc tccgtttttt
     7441 gattaatcaa gcatctaaag cttcttttct tctttcttgg atcgtagcta ccttgacgaa
     7501 cggtagatag atcaaaatag aaagaaccaa attgacagcg gctagtacac cacctgcaat
     7561 actttttgtt gccaatacac cgccaataat tggtggagtt acccatggag gcataacggt
     7621 agctgctggc actaaacctg tagaagtcgc aaagtaagca acactaacta agaccattgg
     7681 tgtcaaaata aatggaatga acatgatcgg gttcaacacg ataggtaacc caaacatcgt
     7741 tggttcatta atattgaaga cacctggcgc aatcgacaag tttgttacaa ccatatatgg
     7801 tttgttttta cgtcctacta aataaatagc taacagtaaa cctaaagttg cacctgttcc
     7861 tcctaagttg acaaagctgt caaagaaagg tttgttcacg ataaacggtg cgctatgtcc
     7921 agctgcgatt gcattaacgt ttgcttcaat ggctggcgca ttgattgttt gcatcaaagg
     7981 atcgaccata ttcgctccgt gtaaaccaaa gaaccataag aatggtgtga taaatgcaag
     8041 cagtaaggca gatggataag agtttgcaag tcccataaat ggttcttgga ccactgtata
     8101 gaaagaacct acgatatcat caacaccgaa tgcagcaaaa attgcagcaa ctaaagcaaa
     8161 cgcagagata gtgatcattg ctggtagtaa tgaagcaaat gcttttgcaa ctgccggtgg
     8221 cacaccatca ggcattttga tgatcaattt ttcatttccg cttaaacgta caaagatttc
     8281 tgttgaaata atcccaacaa taatagcaat aaacaaaccg tttgagctca tacctaatgc
     8341 tccgcctaaa gcgaagaaag agcctaaaga aatcacacct gcggaaacac cgtcttttcg
     8401 gtagccgtta gctaaatgat aagcaagcaa gaaagcaatc aagacagaaa gaatggcaaa
     8461 tgtaccgttc caagcagcag ccccgaatcc tttccatgat tcaccaccaa aaattgagtt
     8521 catcaattct tggaacccgg gaatcggtaa gttattgatc attactgcta atgctcctaa
     8581 gatcattaac ggcatactca ccataaatga gtcccgaatc gcaactaaat gccgttgtgc
     8641 accaattttt gatgctacag gtataaaatg tttctccata aatgcaacaa atttatccat
     8701 aaatcttccc tcttttcttt actgtagatt acgcgttcaa tcgacgatac agatcaatga
     8761 tctcttttgc taaatctgta aaagctatag atgtcatcag atgatcttga ctgtggaccg
     8821 tcaaaagcgt gacttcaacg tgatttcctt gtgcttcttg tgtcagcatc tctgtttgag
     8881 agtgatgtgc ttggactaaa gattcctctg catctttgat tttttcatct gctaaagtga
     8941 aatcaccttt tttcgcagca gcaatcgctt ccattgcatc gctttttgca tttccgccaa
     9001 acatgatcag acccatgatg gcttctagat ttttttgatc ttccatttat tgtatcctcc
     9061 gataattttt attatttgtt tattagtttt tctgcttggt cgagtacttt ttcaccattc
     9121 atcatgccat agtcagccat attgattaca tctaaaggaa tgcctttagg tgctagcttt
     9181 tcagcaaatt gattttgcat aaagcgtact tgcggtccta aaagtaaaac gtctacattt
     9241 ttagattcta gttgattatc cgcatctgaa gcagatacag caaagatgtc tgcttccatt
     9301 ccttttgctt ctgctgcctt ttgcattttt gtgactagga gacttgtact cattccagct
     9361 gaacatacaa gcatgattgt tttcttttcc attttaaaca cgttcctttc tatacttaca
     9421 cttgtatata agcaagtagc gtgccaactt taaaagcgct ttaaaaagtt gatatcatca
     9481 catttaaggt ctataccaag ttgcatacac actaaaaaag aaagcgcaca ctaaacacac
     9541 aaattaattt ttattacctt ttttctttta acgtgcacaa aaaaagttgt gacacaaaac
     9601 ttgtcacaac ttttttcaaa taaacggtat tcaaaagctt gcatctgcgg taataagtcc
     9661 aaccaagtta aaaacataag atgttgtttt tactttgcta ttcttattac ttgatgcagg
     9721 acagcttttt cacaacctct caataaacgg tgttcaatca gctgaccctg ggtattagct
     9781 caaaacgctg gattcacaac ctcttttatt tcaaataacc agtaaataca atgaacatct
     9841 acaaaaaact tctatttatt tatagatttc gtcaggcaaa tccaattctc tcttatcaat
     9901 caaagtctga taccacgaag cagatttttt caatgttctg cttcgttcat cgttcaattc
     9961 gattcgaact aatccatatc gattcttaaa tgcattcatt ggtgacacac agtcagtgaa
    10021 tgcccataac atatagcctt cgcaatttgc gccttcttct gttgctttga tcaagtaatg
    10081 caaatgctgt ccgataaatt cgatacggta atcatcttgc acgattcctt tctcatccat
    10141 atactgttct tcatattctc ttcccatacc gttttctgtt atcagccaag ggatattatg
    10201 ataattttct tttagataca tggctatatc atacatgatt ttcggataga tttcccatcc
    10261 cctggaatta ttcattttct tcccaggtaa atcaaagact tcataatatc gttcaggatg
    10321 gaaaggtgtc tcttcattcc attgataacg aggggcgcga acgcgtttag gataatattg
    10381 attgattcca aggaaatcta ctgtatgatg ctgaatcgtt tctaaatcct ctgttgcagg
    10441 atcgaaataa atcgattgtt tttgacacaa ctccaggaac tcttctgtgt agtatccttt
    10501 taccatcgga tcaaaaaata ctcgattata aaacaaatca tacatataag cagctttttg
    10561 gtcttctgga gaattggatc gagcatagac catttcagga tttaaaatac agccaatcct
    10621 tccaccataa tttccgctgc ggtaaatctc tactgcttta gccgttgcta aaactttatg
    10681 ataattccac atcatccact tttttgtgtt ttgttcatgc ggccagcgaa cagcatccaa
    10741 atagcaacgc gtttggggaa cgattggttc gttgaatgta aaccagtttt ttaccctatc
    10801 accgtaacga tcaaaggcaa tttgagcata cttagcataa aaatcaacta cttttcttga
    10861 agaccagcca tcatattttt ccaaaagaaa agctgggact tcataatgtt ccaaacaaat
    10921 catcggttct acgtctgctt cgatcaaaga gtcgatcatt tcacttatat gctgagcata
    10981 ttcttcatct actatacagt tctcataatc agtaaaaaaa cgagcccaat tgatagaagt
    11041 ccgataatgt gtcagatgga tggatttcat cagatccaca tcctgctggt attgttccat
    11101 aaaattagtt gctacggctg ggccataccc attatgccaa acaaaatgtt catttttata
    11161 ccagctatct ataaacgaat cctgtccttc tttttttcct ttccaacctt ccgtttgcca
    11221 agcagaagat gcagcaccaa gaataaaatt ttttggaata gtaattttca tgatttcctc
    11281 ctgtttttta gatcccatca ttttccacac gatttgctac ttttacaaat ggcagataga
    11341 tcaaaataga aacaataata caaacaagtt gcgtaataac tgcaccgata tttcctgctg
    11401 ttgccagata agcattgacg atcggcggca ttggccaagg gaccattaca actgctttcg
    11461 ccgcaaatcc taaagcggtt gcaaaataac caatcgttcc agtgataaga ggcgttaaga
    11521 taaacggaat agctaaaatt ggattcagca tgatcggcat accaaaaata actggttcat
    11581 tgatgttgaa gatcccagga gcaagggata acttggtgat ttgtttcata tcttcccgtt
    11641 ttccaactaa caatactgca atcagtaaac cgatcgtgac tccagacccg cccatactca
    11701 tatacatatc ccaaaatggc atagtaataa tgttcggtac ttcttttcca gcttcaaaag
    11761 cttccgtatt tacagcaatt gccgctaaaa gcaacggttc ccgtatcggc ttcaccattt
    11821 gatttccatg gataccaatc acccaaaaga cttgcgcaat aaacatcaat agcaaaatac
    11881 ccggtaaccc ttgtacgaca ccttctaatg ggcgttgtac gacattgtaa atgatatcat
    11941 aaaggtacat tcctgtaagt tctttgatga taaatcccgc agccgcgata cccgatacag
    12001 tcagaatcgt cgggaataaa gcagaaaaac tagcagaaac attcgttggc acggtatcag
    12061 gcattctcag ctgtagtttc ttctgtttcc tcaaccagca ataaagttca atagaaagga
    12121 tggagaccaa cattcccaaa aataaccctt tcgtatctgt atattgttta gccaatacgt
    12181 tttctactat aacagtctga ttttcattca ctaattcata aacagttgga ttaacagata
    12241 aataagacat cactgcgatg ataccaggga atacaccatc cactttattc agcttggcta
    12301 attcgatccc tatcagaaaa acagcataga tggtcaagaa acttagtgtg acatagctga
    12361 tggcagatgc gataggcttt aattcttcta aaaaacgcaa gaaacttatc tgtgctaagc
    12421 cattttccga gtcaaagacc attgctgaaa acaatgtgcc aaaagcaccc gtaataatta
    12481 ccggaatcaa tgcagcaaat gcgtttttta tcaccatgat ataccttaaa ttattgattc
    12541 tcgttgccac gttccctaaa acattggcta atttatccat aaaactcatt ccagatcccc
    12601 tcattcctgc ttcttctcct tgacgtcatc tctttttgtc tattagtcag cgcttacatt
    12661 tcgatgaaca gaaactattt actcctcttt tttaccaaat ataaaacaaa aattcatctg
    12721 atgtatttat cataaaatac atcagatgaa ctgtcaatta gtttcttttt ctcctattat
    12781 tttctgtaat tctctccgat ttattgccat atggacagtc attgcatcaa acgcagcaag
    12841 tgcattccgc ctttcgatgg cctgaacgat ctctgtatga atatcgatcg tctcgatttt
    12901 cagatgctgg atatcataat ttttattaaa caaagcaatt gattcattaa tgattgggat
    12961 aatatgcagc aaggctacgt ttccgctagc ttctgcaatc atcgtatgaa aatcgatgtc
    13021 taattgaaca tgttcttgtg tgccttctcg aaaagaagtt tcaatctttt gcgttaattt
    13081 tttcatttgg acgatttgat tcttcgttgc atattccgct gccaaacctg ccatcttagg
    13141 ctccaataaa tatctcagtt caaataagtc tcgtatcatc cgatcctgat cttcaatcaa
    13201 agaaaaaccg agtgggtcat tggcaatccc tcgcttatca ctgacaaatg tccccgctcc
    13261 ttgccgaact tcaaggatat tcctggaagc tagagcccgc acagcttctc gcaccgtgct
    13321 tcgccccacc tctaaataat cggacaattc atattcattc ggcagtttat ctcctacttg
    13381 caactgcatt tcctttaaat atctaaggat cttgtcagta gttacttcta ctagggtttt
    13441 attttcttcc aaaccacata cctccttttc atctgatgta ttttttcgta attattataa
    13501 cacgtttgaa tcatctgatg attttttgta tacaaaattg attaatccgt tcatagacat
    13561 acaaacgaag aagatattta ccataaaaaa gactaggata aaattcactc ctagccttcg
    13621 atcattttta ttttgaacga caaaaaatat gatgttcaat aacagacgat cttcattcga
    13681 ttcttgatgt catcttttgt tttttttaat atatggataa aaagtttgat tacatcgttt
    13741 ctattctaga atttggtcga ttctcaattc ttcttctggt gagaaatcta aattatccat
    13801 cattcgaaca ctttcttgta actgacttaa ccgacttgct ccgaccagta cacttgtaac
    13861 agatttttgt cgcaaattcc atgcaagggc catttgcgct aatgattgtc ctcgtgatac
    13921 ggcgattgtt tgcagtgctt tgattttttc cagagttgat ccaacttgtt cttctgaaag
    13981 gaatggtatt tccttacgat gtgctctaga atcttcagga ataccatgta gataacgatt
    14041 tgtcagcaac ccttgagcaa gcggactgaa cgtgattgcc cctagccctt cttcttctaa
    14101 tactggagat agatcgtctt ctagtagtgc acgagaaaac atattgtagc gcatctgatg
    14161 aataatgaac ggtgcttcct ttctttttaa aatttcagtc attttttttg tatcttctcc
    14221 attatagttg gatataccaa tatataatgc ttttccttgt cttacaagtt gcatcaaagc
    14281 ttctgctgtt tcttccaaag gtgtatcagg gtctgggcga tgatgataga agatgtcaac
    14341 ataatctagc cccattcttt ggagactttg gtcacaactt gaaatgatgg atttacgaga
    14401 gccccattcc ccatatggcc cttcccacat atgataacct gctttgctag aaataatcag
    14461 ttcatcacgg taggcttgaa aatcttctct aaaaatacgc ccaaagtttt tctctgcagc
    14521 tccagcaggg cctccataat tattcgcaag gtcgaaatgg gtaattccca tgtcgaatgc
    14581 gcctcttaaa atctctcttt gattatggat aggatcataa tcaccaaaat tttgccataa
    14641 tccgagagat aaaatcggta atttcaaacc tgaatgaccg gatttacgat attccatttt
    14701 ttcataacgt gcactatctg caatatacaa tttttctcct cctcatcaaa tctttttaaa
    14761 acgctttctt ttttctattt ttagtttact acattttaaa gaaaaggagt taatcaaaat
    14821 gattttttcg tatatttttt tgccatttgt tttttactcg ttgatagatc agaaattgat
    14881 tacgtgcttg ctggattttt ttataacgtt tcggttgttt tttgtaaaat tgctgatgat
    14941 agttttctgc tggccaaaac gtaccggctg gttcgatttt tgtgacgatt ggttggtgga
    15001 aagttcctga actgtctagt ttctgttttg attgttccgc taattctttt tgtcgttcga
    15061 cttgataaaa aatcactgga cgatattgct tccctctatc ttgaaattgt ccaaatgtat
    15121 ctgtgggatc agtaatttgc caatagattg tcagcaactc gctataactt atttctcttg
    15181 tgtcataaat gatctccaca gcttccacat gacctgtgta accaccactt acttgatcat
    15241 aatttggttt ttcaacatgt ccgcccgtgt atcctgacaa aacagatagg atcccttttt
    15301 tcgattcaaa cggctcgacc atacaccaga aacatccccc tgcaaatact gcacgttctt
    15361 gatagtcagc atcttgcatg gcatgcttgg aaaagtcata tttttgctcc ttatcaaatc
    15421 cttccgtaat caataaataa aagtccatta catcaggcgt cagattcatt cggatagcta
    15481 atggacgtag tgttgcttct agttttgata actgttcttt aacggatata ttttctctta
    15541 tgtcattctt tgtctgaatc aatacttttc gttcccaatc tcgtgtattc ggattcaaaa
    15601 tcagattata aagtgtttga atgaatattt gttcctcatc catagattct cctcctttcc
    15661 gaagaagaca agctagtgac tttagctgta atttatcata aagtgaggaa ataagcgatt
    15721 acttcatccc cctacagatt tttcgtataa aaaagagaga atccttatcg acag
//
LOCUS       DAZBSA010000065        15251 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000065 DAZBSA010000000
VERSION     DAZBSA010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.065"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(546..1112)
                     /locus_tag="ACHGMT_001904"
     CDS             complement(546..1112)
                     /locus_tag="ACHGMT_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN6835640.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1112..2068)
                     /locus_tag="ACHGMT_001905"
     CDS             complement(1112..2068)
                     /locus_tag="ACHGMT_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFN6835641.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2068..2724)
                     /locus_tag="ACHGMT_001906"
     CDS             complement(2068..2724)
                     /locus_tag="ACHGMT_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFN6835642.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3094..5112
                     /locus_tag="ACHGMT_001907"
     CDS             3094..5112
                     /locus_tag="ACHGMT_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFN6835643.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5182..5388
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5539..7953
                     /gene="leuS"
                     /locus_tag="ACHGMT_001908"
     CDS             5539..7953
                     /gene="leuS"
                     /locus_tag="ACHGMT_001908"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFN6835644.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8301..9854
                     /locus_tag="ACHGMT_001909"
     CDS             8301..9854
                     /locus_tag="ACHGMT_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFN6835645.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10235..12019)
                     /locus_tag="ACHGMT_001910"
     CDS             complement(10235..12019)
                     /locus_tag="ACHGMT_001910"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6835646.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12034..13755)
                     /locus_tag="ACHGMT_001911"
     CDS             complement(12034..13755)
                     /locus_tag="ACHGMT_001911"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6835647.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13855..14616
                     /locus_tag="ACHGMT_001912"
     CDS             13855..14616
                     /locus_tag="ACHGMT_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFN6835648.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4978 a         2780 c         2991 g         4502 t
ORIGIN      
        1 aatatagcta agattgaaac atccatctgg aatttatctt ttttactgct ttgatcataa
       61 aagtctgcat attcatcact gatatttttt ctaataaatt cttccatcat aaaaatcacc
      121 tcaaaacgat tatacgaact tacgttcttg ttgtaaagcg atatttgagg ggcaaaaaag
      181 gggcataatt tgactgatat taagttagct attgatatat gaatgacata caaaaatata
      241 aaatctttta aaaatgctta tatatcaatg tttttgccat tcttttatat atctgttata
      301 tgtcaaaaaa atgtgttcaa tttcaatttg tatttgaata aagaggttgt gaaaaagctg
      361 tcctgcatca agtaataaga acagccaatc aaaaacagct tctcatgttt ttgatttggt
      421 tggacttatt accgcagata taagcttttg agcaccgtga ataaagaggt tatgaaatca
      481 acgctctaac ctgagtatta gagaaaaaaa cgcaaaacat atcatttatg ttttgcgttt
      541 tttatttatc tttttttctc aatacaatat aaaataggcg gctggttctt ttggttgata
      601 aaccgataat tcagcacatt atatgtttcc tgtggcagtt tggaacaaaa agaatcaacc
      661 atatccagtt ctttttctcc accttcatga ccatagtaga cgacaaggat caatcgtccg
      721 cgaggcacaa gtcgtttcaa tatttcttcc atagctgtgc gtgtagtctc tggcatagtg
      781 atgattgctt tatcactttt tgggagatac cctagattga aaatccctgc tttgagattt
      841 tgttgttcag ctatcacttc tcctaacgtt tcgtggcctt gtagaaacaa tgagacacgt
      901 tcttctaact gacgctgttc tagcctttca cgagtagcat cgattgcttg ttgctggata
      961 tcaaaactat agacatgacc agtttttccg atgtgttctg ctagaaaaac cgtatcgtgg
     1021 ccattgccca tcgttgcatc tacgacgtga tcccctggtt gcaagatttc ttgcagtaat
     1081 gtatggctga aatgcaaagc agtttgcaac atcagacgct cacctttttc cgtacatcat
     1141 attttccttg gaagctatct ctgcgaagaa gttcttcatc aattgcattc aagacttccc
     1201 attttttcaa gctccacatt ggtccaatca aggaatccca cggcgcatcc cctgtcaaac
     1261 gatggatgat gatctctttt ggtatcattt ccagctggtc gcaaatgaca ttgacatagt
     1321 caggacggct cattaattga aggcgacctt catgatagtc ccgcaacatt ctcgttttgc
     1381 gcatcagatg catcaaatgg agcttgattc cttggatatc tgaatctaaa atggtgcgtc
     1441 tgacattttc tttcatcatt tctaaacttt cgcctggtaa tccgttgatc aaatgcgtac
     1501 aaacacggat attatgtttg cgtaatttag ccacgccatc taaataagtt tgataatcat
     1561 gtgctcggtt gatcagtttt gaagtttctt caaaagttgt ttgcaatcca agttctaccc
     1621 ataaatacat ccgctcgttc agttcagcaa ggtaatcaac tacttcatct ggcagacaat
     1681 ccggtcgtgt accgactgat aatcctacga cacctggtaa attgactact tgctcaaatc
     1741 gttctttgat tatttcaact ggcgcatggg tgtttgtaaa attttggaaa taaacgatgt
     1801 attgttggac atgaggccat ttttgatgca tcatgtcgat ttctttctgg aattggattg
     1861 gaagagggtc ttcaggtgca acgatcatgt ctcccgaacc agatacactg caaaaagtac
     1921 aaccgccatg ggcaacagtc ccatctcgat tcggacagtc aaatcctcca tcgatcggca
     1981 ccttgaaaat tttcccgcca aactcatttc gtaaagcgta attccaagaa tgatatcttt
     2041 tatttccttc tgaataagga aaagtcatta aaagtccttc tttctgtaag agttatctat
     2101 ttgttttctt ttttctctat acactggata agtcaggcac agccaagaaa gactgagtaa
     2161 gtaaccgccg atgatatcag taggaaaatg aacgcccaga taaatgcggc ttatcccgac
     2221 acttactata ataaacatca tcagcaattg aagcaatgtg cgtaaggttc ttttttgaac
     2281 aaaagctgga agcagaaata gtaaagaacc gtaaagaatc atactgcctg ctgcatgtcc
     2341 actagggaaa ctgtaacttg tttcaactac taagtgcgcc aatgaaggac gctctcgcat
     2401 gaaaaatagt ttgatcaccg ggttgatgat tccagcaatt acaataaatc cagaaacgaa
     2461 ccaaaatgtt tctgcccttt ttttcttata ccacatccat aaagccgcac aagcaaataa
     2521 aatgatgacg gtaagaggat taccgaattt agtcataaag agttggtagc tgttcagatc
     2581 aggataagaa gctctgacaa tgcctgtaaa ccactgatcg aaaccagaaa gccacgacag
     2641 atggaacttt acgatccagc ccaaaatgaa aaacaggaca agaaagaagc taccagccac
     2701 ctgaaatgat gttttatatt tcatacaatt cctcctttga agcaatgact tattatagca
     2761 tttttggtcc agcgaaaaaa acggtaaaga gataaaacca atgtatgatt cctctttgaa
     2821 taaccaatat tcttataaaa aaaataactg taatcggaaa gacatttttt ggtaatgcaa
     2881 atgaaatgtt agggaaataa aatcagtgtt tttcgaaatc cttttaaaca agaacgtaga
     2941 cgtaggaaga acttgaaaat gaacagaagt tgttcattac ggtaaattac aatatgatta
     3001 caaaaaacaa caaagctctt aaataacttt taataaatat taagagaagt tataattatt
     3061 aataatagat aaattgaagt tggaggatag aagatggaaa acattgcaag aaaagaacgg
     3121 agacgtttag aacagaccaa acgttttcga aaagcaaaac gaggggcagc aatcgtggga
     3181 actgcgatgg taggctgttc tgtcgctgcc ccattagtac agccggttcc tgttcaagcg
     3241 gatgaaacac ctgttcagtt cggcgcaaga atcaatacag cggcatttat cacagaaatt
     3301 gctacttatg ctcaaccaat cgctcaagcc aacgatctat atgcatcagt gatgatcgca
     3361 caagcagttg tagaaagcgg ttggggcagt agtgcgttat ctcaagcacc ttattataat
     3421 ctttttggga taaaaggcag ctatcaagga caaaccgtct acatggatac gcttgaattt
     3481 ttaaataatc aatgggtaaa taaaaaggag cctttccgac aatatccttc ttttgctgaa
     3541 tctttcaatg acaatgccta tgtacttaga aatacttcat ttggcaacgg ttattactat
     3601 gccggtactt ggaagagcaa cacgaaatcc tatacagatg cgacagcttg tttgacaggg
     3661 cgttatgcga ctgatcctgg ttacgcaggc aaattaaata atatcatcgc aacttacgga
     3721 ttgacccaat atgatacacc agctagcgga aacgcaggcg gtggagcgac tacaggtggc
     3781 aataatactg gaagtgcagg tagtactggc ggttcaagca attctggtaa tacaaactca
     3841 ggttcagcaa cttcgaccac ttatactgtc caatctggtg attctgtttg gggaatctca
     3901 aataaattcg gtatcacaat ggatcaattg atccaatgga acaacatcca aaacaatttc
     3961 atttatccag gacaaaaact gacaatcaaa ggtagccagg aaaatggctc ttcgacgaat
     4021 aattccggta acaataccaa ttcttcagga aatgctggta catcaaacgg aggacaaaca
     4081 actggtgcga aatacactgt tcaatctggt gattctgttt ggaagatctc aaatgatcac
     4141 ggaattacga tggctcaatt gattgaatgg aacaatatca agaacaattt cgtttatcca
     4201 ggacaacagc taatcgtatc aaatggcggt tcttctaatg ctggagcaaa tactggctca
     4261 aatactggga atacaagtgg ctctagttcg tccagctcaa gttcttctaa tacagcttca
     4321 ggaacgaaat atacagttaa agccggagac tctgtttgga gcgtctcaaa taaatacgga
     4381 attacgatgg ctcagctaat ccaatggaac aatatccaaa ataactttat ctacccagga
     4441 caacaactga ctgtttcaaa cagtggatca acaaacagca cgtcaggtaa tacaggttcg
     4501 tcaaccaata caaataacag cagttcagtt aatactggca gcagttcgtc tcaaggcact
     4561 tataccgtga aagcaggtga atctgtttgg agcgtggcaa ataaaaacgg catcacgatg
     4621 gatcagttaa tcgaatggaa taatatcaaa aacaacttca tttatccagg acagcaattg
     4681 atcgttaaag gtggaacttc ggcaaataca aatacaggtt caactacttc tgcagcgaaa
     4741 ccaaatacac cgaatacgtc tgctacgaca agtacatcat ccactggtaa tacgatgtat
     4801 accgtgaaag cgggcgagtc agtttggagc gtagcgaaca aacatcatat cacgatggat
     4861 cagttaatcg aatggaataa tatcaaaaat aatttcatct atccaggaca gcaagtgatt
     4921 gtgaagaaag gttcatctca atcaacaaac caacaatcag ttactggttc gaaaacctat
     4981 actgtgaaat ctggagaatc tgtttggagt gttgctgaca gtcatggaat tacaatggct
     5041 caactaatcg aatggaacaa tattaagaac aactttattt atccaggtca aacgttgatt
     5101 gtagctaaat aaaatagctg ttcgaagaaa taacgattga attccacaaa aatatttgtt
     5161 actataaggc tacttaaata tgacaacact tgaaaaggaa gtagtaaaaa gactgaagtt
     5221 tcagagagcg catggctggt gagaatgtgc acggatgctt ttgaactcgc ctttgagttg
     5281 cccttgctga atacccgagt aagcaagcgg cggtgacggc cgttacaacg ttcgaggcct
     5341 tttcactaag gtaaaattag gtggtaccgc gtagattacg tcctataaaa agagcaatct
     5401 ttttatagga tttttttatt tcttttgtga agtttgagaa aacaaagcaa aaggaaataa
     5461 aaataaacag attctgccgg ttttttcgtt atatttataa tagaaacaag tgttttcaat
     5521 agaacaggag gacagcaaat gagttacaat cataaagaaa ttgagaaaaa atggcaaaaa
     5581 tactgggcaa aaacgaatac tttcaacacc catgatgatc ctgaaaagcc aaaattttac
     5641 gcactggata tgtttcccta tccatccggt caagggctac atgtagggca tcctgaaggt
     5701 tatacggcaa cggatatcct ttcacgtgtg aaaagaagtc aaggatacaa cgtgctgcat
     5761 ccaatggggt gggatgcatt cggtctgcct gctgaacaat atgcgctaga tacaggaaac
     5821 gatccagctg agtttaccca aaaaaatatc gaaacattcc gacgccaaat caattcttta
     5881 ggatttagtt atgactggaa tcgcgaagtg aatacaactg atccggagta ttataaatgg
     5941 acacaatgga tctttacgaa gctttatgaa aaaggtctag cttatgaagc agaagtagcg
     6001 gtaaactggg tacctgaatt aggaacagtt atttcaaatg aagaagtgat cgatgggaaa
     6061 agtgaacgtg gtggctatga tgtagtcaga aaaccaatgc gccaatggat gctgaaaatc
     6121 actgcttatg ctgatcgctt attggatgat ctagaattag ttgattggcc agaaagcatt
     6181 aaagaaatgc aaagaaattg gatcggtcgt tcagtaggag caaatattga atttaaagtg
     6241 gctggtacgg ataaatccta taccgtattt acgacacgac cagatactct attcggtgca
     6301 acttactcgg ttcttgcacc tgaactagat cttgttagag aaattacgac ccctgaacaa
     6361 aaagctgctg tagaagctta tatcgaagag acagctaaaa aatcagatct aaagagaacg
     6421 gatcttgcaa aagaaaaaac aggagtattt actggtgctt atgcaatcaa tccagttaat
     6481 ggcaaggaaa tcccaatctg gatcgccgat tatgtattat cttcatacgg cacaggagcc
     6541 atcatggctg ttccagcaca tgatgaacgt gactttgaat tcgctcaaac tttcggacta
     6601 gaaattcttc cagttatcga aggtggagat gttgaaaaag ctgcttacac agaagatggt
     6661 acgcacatca actctgaatt tctagacggc atgaacaaac aagaagcaat cgataaaatg
     6721 aatgaatggc tagaagaaaa tggcgtaggg aaaaaagaag tcagctatcg cttacgtgac
     6781 tggctattct cccgtcaacg ttactggggt gaaccaatcc caatcatcca ttgggaggac
     6841 ggaacagtca cagctgtccc agaagaagag ctaccattac ggttgcctaa gacaaaaaac
     6901 atcaagccaa gcggtactgg agaatctcca ttagcaaata tcgaggagtg ggtcaatgtc
     6961 gttgatcctg taacaggtaa aaaaggccgt cgcgaaacga atacaatgcc acaatgggca
     7021 ggaagctcat ggtattacct gcgttatatc gaccctcata acaaaaaaga actagcagat
     7081 tatgaaaagt tgaagcgttg gctaccagtt gacatctaca ttggtggagc agaacatgcg
     7141 gtacttcatt tactatatgc acgattctgg cataaattct tatacgatat cggtgttgtt
     7201 cctacaaaag aaccattcca aaaactatac aatcaaggaa tgatcttagg cgaaaacaac
     7261 gaaaaaatgt caaaatctcg tggtaatgta gtcaatcctg atgatgtggt cgaaacttac
     7321 ggtgcagata cactgcgcat gtacgaaatg ttcatggggc cattagatgc ttcgattgct
     7381 tggagcgaaa atggcttaga aggaagccgt aaattcttgg atcgtgtatg gcgtttgatc
     7441 gttgatgaga acaataaaat gcgtgaccgt atcactacat tgaatgatgg caagcttgat
     7501 aaagtgtatc atcaaacagt gaaaaaagtg acagaagact atgaaaatct tcatttcaat
     7561 acagcaatct cccaattgat ggtctttatc aatgaagcct ataaagtgga cgctctgcct
     7621 tatgaatata tcgaaggatt tgttcaacta ctcgcaccga ttgctcctca catgggtgaa
     7681 gaactatggg caatccttgg aaatgatggt ggtatttctt acgcaccatg gccaacatac
     7741 gatgaagcag cacttgttga agacgaagta gaagttgttt tccaagtaaa cggtaaagtt
     7801 cgtgcgaaaa gcaatgttcc ccgagactta ggaaaagatg agctagaaaa agcagcatta
     7861 gccaatgaaa tagtgcaaga atatattgaa ggcaaaacag tcagaaaagt tattgcggta
     7921 ccaaacaagt tagtcaatat cgttgctaat taattggctc tttgtcaata aggactgatg
     7981 agttgtgcaa aatcaaatct gggccagaat gaacttcatt ctgtctcaga ttttttgtta
     8041 tttcaaattg attaattgat tgatcaaaaa ggttttgatt ttcatgttct caaacacgtt
     8101 caatgtcttt ttcttctgaa tggtcttgta agagatctct gtaatcatca aatttttgtc
     8161 tattaatctt agtatttttt gatgtataaa ctacctgaaa ttgagagtac ataccaaatt
     8221 cataaatttt ttattagtgg gactagtgat tgtatggatt ttgacgcata atgaatgaag
     8281 tatgagaaaa ggagagacaa atggatcaag aactatttat tatcgaagta ttatcagcta
     8341 aacaaaaaat tatcgagaaa gcgaaaaaaa gagcgaaaag aataatgttc aagcttttaa
     8401 gcttagtatt tttctctggt ttgctttttt tcggagctgt atcgttccta tatcgccata
     8461 tgagtgcagt agtccgttca gctgaaggga tgcaaaaatt aaataatttg tatcactttt
     8521 cgtggggagt tggcggaata acaattgttg tgcttattgt ttttcttcta cttatttggc
     8581 tccgttacca tcaattgtcg cgtcatttcg atcaatggct tagtcaaaga gcacaacagt
     8641 ttttacataa taaaccaact tctcaagatg atgactatct ttattttgag aagacttcga
     8701 cgagtcaagc ttttcggata caaaaaagtg attgccagtt attgctgaga tcacttgatg
     8761 atttacctat ctacttaggc gaaagcaaat caaaattcgg aatgaacaca ttccatattt
     8821 ttttactaga aaacgaaccc agatgggaaa aaagccagtc ttcgcctggt ggatacctga
     8881 caactaagat gagtacatta gtcactggtt ttttcctcat tcttttagct ggaacaatct
     8941 attatgggag atacactcag ccaaaaatgt cttctcatat aaatgctgct tctcacatga
     9001 caatgaactc aactaacgat acgcatatac aaaagacaaa tgaagggttg ttagctgatc
     9061 ctaagcaaaa ggtaaatttg acaacacaaa agataaatga tctcaaactt gatacagaga
     9121 cgcactctct ctatatgaca acagatagtg gtgctaattg ggtatttgtg ccgattgatt
     9181 cgcaatggtt gcgttttggc gaatacaccc tgacaacggg gctgattcct gaaggttatt
     9241 ggatggacaa cacatatgat atttccgccg atttctcatg gtttatctat tcttctgggg
     9301 atgatgtacg tctgctgact tcaagagata atggtaaaac atggcaaacg tctattatca
     9361 cccaatcagt tcagccgata cgttatcgaa aagcggtatt ttatggcaac gatggtctat
     9421 ttatctgtac atcagccagt gggatgtctg cagaaacttt gtacatttac actactatgg
     9481 atggcggaat cacttggata aaaagtggaa gtacaatgat caatcagcca gtacaaaatg
     9541 tcagcttcgt tacaacttct ttaggttttg tttcgacacg tgaaagtttg ttttatacaa
     9601 acaacggagg acgctcattc aaagaatcag ttctgtcgat tccggaaaac tatcagacag
     9661 gggggataga tctttttcaa tcaccaaatg aagttactca aataagcgcg aatacgttag
     9721 agacaaaatt ttatttgttg aaaaaaggac caatcgatgc agggaaaatg tttgcctgcc
     9781 tcttccgttc aactgataac ggggaaacat ggcaattcgt tcagcaacta tcgcaagtag
     9841 aacaaggaga ttaaagaaga gagattgtga gcctgacgtt cagcaccgag tcacaagaaa
     9901 caaattcaaa aaacagcttc tcatatttgt tgaatagtgt aactcgtgat tcggagttga
     9961 caagagcaac aagaagcgat tttgaagtat agcttctcgt attttacaag atcgtgtctt
    10021 attgccgata ggttgtctct tgaacaccgt ttattcgtaa aaagggattg tgacaagtct
    10081 tttgtcacta cttaccgaac gaaactgaag gagctatcca aaaagtatca ttagtattat
    10141 tctttgtgtt tctagtggca ccactagtac atttaaaaaa gctatcgatc atggactaat
    10201 catacatgat cgatagcttt ttagataatg tttgttacgc tatctgaaat tgactattat
    10261 aaagactggc ataaagcgtt ggtgattgga gtagttcaga atgtgttcct ttttcgacga
    10321 tatcgccatt cttcattaca aggatcatgt ctgcattttc aatcgttgac aaacggtgtg
    10381 caatcacaaa acttgtccta tcttttgtca cagcttccat tgcttgctga atcaatgctt
    10441 ctgttcgtgt atctacgcta gaagttgctt catctaaaat aacgacagga ggattcgcta
    10501 gaatgactcg agcaattgtc aataattgtt gctgtccttg agataacgtg ccgttttcgc
    10561 tagaaatgat cgtatcatat ccgtctggca gtgttcgaat aaagtgatca cattgtgcga
    10621 tttcagccgc ttcgataatt tgttctcttg tggcatccat tttcccgtag gcgatattat
    10681 cagctaccgt tccttcaaac agccaggtat tttgtaaaac catcccaaaa gaacgacgta
    10741 gttctccacg tgaaagcttg gtaatatcaa gaccgtcgaa ttcgatcttt cctttattca
    10801 gttcataaaa acgcatcagc agattgacta aagtcgtttt tcctgcacca gttggaccaa
    10861 cgatcgctac cgtttgtttt ggctttactg aaaaatcgac ttgattcatc aatattttat
    10921 ctgatgtgta accaaactgt acatttttga aagctatttt tcctttcggt tgttctaaaa
    10981 gtcttgcatc tggaaggtct ggaatttcat ctgtttcatc taatatctca aatatcctat
    11041 cgattgctgc cattgcggat tggatcgagt tgatcacata agaagcggta gaaattggtt
    11101 cagaaatctg attgatgtac tgaagataag cttgtaagaa gccgatcgtc acaccacctg
    11161 atagaacaag catagcaccg aataccgcac ttatgatgaa ggcgagttga ttaatgaaac
    11221 gaatggccgg atagatagca cagttcataa attgagcacg tttaaaagcg ttgtattgct
    11281 ttttatttac ttcgtgtata gcagcttctg catgagcttg ctgattgaat gttttgacca
    11341 ctagattacc agctaagatt tcttccattc gattatttaa aacacccagt tcatactgat
    11401 tctgttcaga aatctgtttg tttttgttcg caatcttagt tgtaacaatt gaactgccaa
    11461 cgatcagcaa taagacgagc aaagtaagct tgctttcgat ataaaacagc atcagaccag
    11521 cagcagctat tgttacgaca gaagtaaaaa attgattgat acctgtcaat aacacttgag
    11581 aaagttgatt gagaccggta gttgtccgac taatgatgtc acctacttga tgattgtcaa
    11641 aaaacgacat cggtaatgtt ttgaattttt ttgtgatttc ttttcgcacc ctcaatgtga
    11701 tcttttcgct taacgctgcc attgtccgtt cctgaataaa tgaagtgatg ctgcttaaga
    11761 ctgaacagcc gatcagcaat aagacaggga acaacaatgc ttgtttgata tcactgaaag
    11821 aaagctgtcc aatacctgtt tttcttattt ctgataagag atcatcgatc ccgattccca
    11881 tgatcaaggg catggctacg actaataggt tcccgaataa actgcataga aacaatccgt
    11941 aaaaatattt atgttcaggt ttgatcattt tccaaaaacg tatcaaagca cgcgtgttca
    12001 acgttttttt cttttccatc gtatcccttc tttctatatt ccttgtgatt ttgcaaactc
    12061 ctgataagtt tggcaattag acagtagttc ttcatgagta ccttgtccaa cgatttttcc
    12121 ttcatctaaa acaatgatgt tatctgcttg ttggattgta ctgaggcgtt gagcaacaat
    12181 cacgagagtt ttctccgaca tgtgtttatg aagggctgct cttaatgctg catctgtttt
    12241 atagtctaaa gcggagaaac tatcatcgaa tacatagatc tctgctggtt tgatcaacgc
    12301 tcgagcaata catatccgct gtttctggcc ccctgaataa ttagaaccgc cttgagcaac
    12361 aaagctgtcc aatccgtcag gaagggtgtc cactagttct tctaattgag cgatcttgat
    12421 cgctttagac aactcttctg ttgaggcata ttcatttccc atctgcaagt tgcttaaaat
    12481 tgtcccgctg aataagaaag ccttctgagg gacataactg atgtgggagc ggatctgttc
    12541 ttgagtcatt tgagtaagag gaataccacc aagggttact tttcctatcg tagggtcact
    12601 aagtctcaag agaagtttcg ccaaactact ttttccagat ccggtcccgc caacgatcgc
    12661 tgttgttttt cctttaggta ttgtgaaatc aatatcttct agaactggat cagagatttc
    12721 agtatattta aatgtgacat gttcaaaagc agctgcattt gtctgatcaa aggttggttc
    12781 agctactgtt ttgatcgtct cttcctcaat ttcgatctct gcttgcagta tttcttctat
    12841 ccgtcgtaat gaggcaaacg atttagggat aatgaccaat accatagctg cgatcatcaa
    12901 gtaagcaagt gtcagcatag agtattcgac tacagcagtc acgacaccga tttgtaatgc
    12961 tccttctgct acaagatttc cgccaaacca caaaatagaa gcataaacga ttcccatgag
    13021 caaaaaagca attggagtga cccaagcaaa tattcggttc actttgatca tttgattagc
    13081 atagtcagca aagctcttat tcgttcgctt ttcttcatat gattgattat caaaggcacg
    13141 gatcatatta atcccagtaa agaattctct caatgtgatg gtgatccgat ccatttttgg
    13201 ctggatactc aaagataaag gtgcccctcg tttcatcagt cgataaacaa tccatacata
    13261 aaaaacaaca gagatcaatg taacccatgt tagtaataga gaataacgaa acatcatgag
    13321 aatggtgaaa acgcaaataa ccggtgctgg taagatcatt tggcaaaata aaacaatcat
    13381 ttgctggaca ttatcgacat cattcgtcat tcttgtcaaa agagatccag aactaaaacg
    13441 actgatatcc ttcattgaca gctgttggaa tttacggaaa tatttctccc gcgtttggaa
    13501 tccaaaccta gcggaagcca atgctgaaaa gtaactagag gcaactgcag cgattgttcc
    13561 tagaaacgct acaagcagca tttcaactcc aatttttttt ataactgagg cttcgccact
    13621 tgctatacct gtgtcgatca gctttgctac taattttggt acacctaatg taccggcgat
    13681 ttctaaacag atcatcacaa aggtaaggag agcgagtcct ttgtttttct gtaagaatgt
    13741 ttttaagata gacatattat tcctcctttt ttcctgatac ttttcgattc tataccttca
    13801 agtaacttga aggtcaatag aaaaaagaat aaactgaaaa agaggtgaga aaaaatgaaa
    13861 aaaatgtata cgattggcca agtatcggaa ctatttgatt tgccaaaatc aacgatccgc
    13921 tattgggatg aacaaggatt aatccgttca agtagacaag aagagaatga ttatcgattg
    13981 tttgatatcg atgatatttt tatgctttat gatatcgttt tttatagaaa attagatata
    14041 ccgatcaaac agatgaagaa tttatatgga aaaacattga cggagttata tgagacactt
    14101 gatgaaacag aaagaagaat ccataaagaa ctggtggtga tgaaacaaaa acaaaaagag
    14161 ataagggagc aaaaaaaaca gttgaaattg atgatcgata caaacgagga agaatttcct
    14221 gtagaagaga tcccttttga ctgtatgatc tcaacggagt ttgaagatat tgtggaaatc
    14281 aaaaaatttc tacctaacta ttcaagcttt gggatgatga gtatgccagc ttccagttca
    14341 cagacgatgt atggattttt tatcgaccct agtgaagtac atttatttac tcaagacgtg
    14401 atttgggaga aaaaagatac ggctgtttat cgtcgattct tattgaaatc cgagatgaac
    14461 catgcggaac gtaataatat tttagaaatc agagaaagaa tgtatgaaaa aggatggaaa
    14521 acaggagaag tgatcggcca gtatctattg acgaatacgg atgaaaacaa tatcagaact
    14581 gaatattatc atgcatggat agaaatgaaa aaataggaaa tatgtgttta agataactcg
    14641 aaaatgatgg ttgttttagt tataaagaaa aattttctgg ttcgattacg ggtttaatgt
    14701 gggggattat tagtcgtgga tagatagaat tttagttttt tagaggaaat atgtctttaa
    14761 aaatggaatg gttgcaattc ttagcgatat atacgatgta attgtcctct taaaaaagta
    14821 tggaaaaata attgtattgg gtgttatgta taattcatgg tgaactgaga ctaatgagaa
    14881 gaaaaattca tattgttatg agtgagattg tatcagatga gaatgataaa ttaaataggt
    14941 gcataaaagc attacactta aaatacattg ttgcttacat tattacaata tgttaataag
    15001 gcaaaaataa atagatggat tgaataaaca tattttttaa tcaaatagtc cctactgtga
    15061 ctttcctcag ctatcggtga tactaccgat agcatttttt tgcttataaa ataattataa
    15121 gtatttagag atttttcttc taacattctt caaacaagaa aaatcacaaa ggttattgtt
    15181 atttttatat attcttttta agaatgattg tttttttata gaaattttaa aaaataaaac
    15241 acgttattga t
//
LOCUS       DAZBSA010000066        15218 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000066 DAZBSA010000000
VERSION     DAZBSA010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.066"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            345..677
                     /locus_tag="ACHGMT_001913"
     CDS             345..677
                     /locus_tag="ACHGMT_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN6835649.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            587..781
                     /locus_tag="ACHGMT_001914"
     CDS             587..781
                     /locus_tag="ACHGMT_001914"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN6835650.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            820..966
                     /locus_tag="ACHGMT_001915"
     CDS             820..966
                     /locus_tag="ACHGMT_001915"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835651.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            1404..2744
                     /locus_tag="ACHGMT_001916"
                     /pseudo
     CDS             1404..2744
                     /locus_tag="ACHGMT_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2985..4601)
                     /locus_tag="ACHGMT_001917"
     CDS             complement(2985..4601)
                     /locus_tag="ACHGMT_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFN6835652.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKKIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            4719..4937
                     /locus_tag="ACHGMT_001918"
     CDS             4719..4937
                     /locus_tag="ACHGMT_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6835653.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5132..5593
                     /locus_tag="ACHGMT_001919"
     CDS             5132..5593
                     /locus_tag="ACHGMT_001919"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN6835654.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVTGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5786..6025)
                     /locus_tag="ACHGMT_001920"
     CDS             complement(5786..6025)
                     /locus_tag="ACHGMT_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6835655.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(6049..7572)
                     /locus_tag="ACHGMT_001921"
     CDS             complement(6049..7572)
                     /locus_tag="ACHGMT_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFN6835656.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(7590..7712)
                     /locus_tag="ACHGMT_001922"
     CDS             complement(7590..7712)
                     /locus_tag="ACHGMT_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFN6835657.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(7742..8725)
                     /locus_tag="ACHGMT_001923"
     CDS             complement(7742..8725)
                     /locus_tag="ACHGMT_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFN6835658.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(8749..8898)
                     /gene="rpmG"
                     /locus_tag="ACHGMT_001924"
     CDS             complement(8749..8898)
                     /gene="rpmG"
                     /locus_tag="ACHGMT_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFN6835659.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(8919..9296)
                     /locus_tag="ACHGMT_001925"
     CDS             complement(8919..9296)
                     /locus_tag="ACHGMT_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835660.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(9328..9507)
                     /gene="rpmF"
                     /locus_tag="ACHGMT_001926"
     CDS             complement(9328..9507)
                     /gene="rpmF"
                     /locus_tag="ACHGMT_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFN6835661.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(9522..9791)
                     /gene="rpsN"
                     /locus_tag="ACHGMT_001927"
     CDS             complement(9522..9791)
                     /gene="rpsN"
                     /locus_tag="ACHGMT_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFN6835662.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(10314..12080)
                     /locus_tag="ACHGMT_001928"
     CDS             complement(10314..12080)
                     /locus_tag="ACHGMT_001928"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6835663.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(12040..13794)
                     /locus_tag="ACHGMT_001929"
     CDS             complement(12040..13794)
                     /locus_tag="ACHGMT_001929"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6835664.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(13904..14473)
                     /locus_tag="ACHGMT_001930"
     CDS             complement(13904..14473)
                     /locus_tag="ACHGMT_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFN6835665.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(14491..>15218)
                     /locus_tag="ACHGMT_001931"
     CDS             complement(14491..>15218)
                     /locus_tag="ACHGMT_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFN6835666.1"
                     /translation="NNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINF
                     DVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFF
                     TESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKT
                     NLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGI
                     KKDIKLRVEDSTLIEQIFNDITK"
BASE COUNT         4848 a         2959 c         2290 g         5121 t
ORIGIN      
        1 ttagcttaaa aataactttt gtcacagtct cactcctaaa attactttat ttacgtatga
       61 agtgggcagg aggtcgaatc cagaactggg ctatggtact tcaacgagga cttctcctct
      121 agaattgaaa agcatgcgat ttaccttccg taacgacggt agatttattc aactatgtgt
      181 ccaatcaaaa tttgtctgat ttcaaaaata gtcaagagaa ttttggttgt taaaaactct
      241 tatctatcaa cttccacagt ttagttgaca aaccctgata ctaagttggg ctatgataca
      301 cttttcttga ctatcagtta cgttaccgga aggaatgaac tctattgaga aaaacatatg
      361 atcgtaaatt taaactaaag atttcacagg atatccttga gaaaaaagta actaccaaga
      421 aaattgctga agaatacaat atctctcgtc caacaatttc ccgatgggtt tcagaatatc
      481 gtcgatacgg gaaaaatgct tttgctggac aaggaaaaag attacctgat aaagcagatt
      541 tctacattct tgaacaggaa aataaacgac taaaagagga agacgagtgt tctacgaaca
      601 caaagatcga tacggatctc cagggatcac tcaaaaactt tacgatgaag gaatcgaaat
      661 caataaacgt gttgtagctg ttcttatgcg tgaaatgaat ttatgcacaa gaggatttca
      721 tcgacgcaaa tcttctcatg gtagagaaaa aacaatcaaa gaaatggtaa aaaaatgttg
      781 aatcgataat ttgaacagaa ccaaattgat gctgtttggg tgatggatat tacttacatt
      841 ccatgtaatg acggtcgcct ctatttgtcc acctatattg acttagcaac tcgtattcta
      901 cgttactata tggtagattc acacatgaaa aaagagattg tcgtccgtcc attacaaatt
      961 tattaaggag aattgcctac ggtaattcat tccgatagag gtagccagca tcggtctttt
     1021 atttatcaag agctgttgaa cgaccaccac atcacacatt cagtgagcca aactgggact
     1081 cctgttgaca atgcagtaat tgaagcgttt catcgatcaa tcaaatgaga attaatcgac
     1141 tccaacaaat ataaaagtaa aatagagatg aaggtgttaa tccaagatta tttagaagac
     1201 tactacccaa acaaaagaat ccatacaaaa tttatgatga ctccttgaca atttgaagcg
     1261 aagaaaataa gggatctata atatctagtg ttggtgacgc cttctgttgc caatactttt
     1321 tcttttttct aataaacgca aacagacacc ttaaaatcct ttagaataaa gtcgacgaaa
     1381 accattcaaa ggagtgtttc aagatgtcct attcagagtt taccaaagag ttactagata
     1441 ttctagacct aaatcttatc tttcatgaag atgcctttcg caaagagcga atcaatgacg
     1501 aaacatgttt tgtttttgat ggcacattga cctatcaacc ggaggaatgc tttcatcgcc
     1561 actatcaaaa tgaacaaacg atcattaaat agggctggaa aaaagtatcc atcttattga
     1621 atgatgtcag taactacaag accattctgc ggattaacaa acagcgcttc aaatgtaaac
     1681 actgtggaaa gactttttta gcagaagatt cagttagtga tcgtcgctgc tccattgctc
     1741 gcagagtcaa acaagccatt cttgagctat taagcgaacc tctttctatg tctttgatcg
     1801 cacgtatgaa gcacatttct ccgaccactg tcattcgaat acttcgcagc cttcggccta
     1861 aaaccatttc tttgagccag ctgttaccag aagtggtgtg tttcgatgaa ttcaaatcag
     1921 tgaaaaatgt ctcgggagcc atgagctttg tcatgatgga cggcaagacc catcaactga
     1981 ttgatattgt ggaaaatcgg caactcaatc ctttacgcga ctatttcttg cgctacccta
     2041 taaaagtccg tgaacaagtg cgtctagtgg tttcagattt ctatacaccc tatcgcacat
     2101 tggtgaaaga gtgttttccg aatgcccgaa tcgtcgccga tcgctttcat attagccaac
     2161 acattggtcg ggcttttacc aaccaccgaa ttcaagtaat gaagaccttt aaaaaagggg
     2221 atcgtcggtc gaagcactta aagaaatact ggaaactact tcaaaagaat gcgtgggaat
     2281 tgaaaggtca gcatcgctac tggcgtccgt catttcgaga tcatctgaca gaagcagaga
     2341 tagtcgatcg gctattatct tatgatgatt ccttaaagcg aggctacgaa gtctaccagg
     2401 attttctttc cgttatcaga agacaagatg ttcctgaatt cgtcgcgctc ctaaaagaag
     2461 actacaagga gttgccagag cactaccaac cagtgttcac gaccttcaag aaataccaaa
     2521 cagaaatcaa acgagcgttg cgcgttccct attccaacgg ccctatagaa tgtttgaata
     2581 accatatcaa agtactaaaa cgaattgcct atggctatag aaactttcaa aactaccgag
     2641 aacgaatctt tttatatcga ggaaaatatt ttaagaaaac taagaatact acccaactga
     2701 ctaaagccag aacaacaaca cggctagaca agatagctgt ctagccgtgt gagtatctcg
     2761 attattctat tgaatttctt caccaacact cattgacgaa gagccaaaat aagtaatcta
     2821 accgactgtt aactaaacca ttgcaggtcc cgttaaagat acttgtgcct gttgtcaccg
     2881 cttctttttc ttgtccacaa gcgcttaaca cacttaataa agcccctgct attattaata
     2941 ataatatctt tttcttatta aaaacctcct catctatttc cagattagta aatgtagtaa
     3001 tctgtgcttt cttttttctc aatactaata actaacttat ggggtaaaca aatactcgtt
     3061 tgaccgggtt ttgagatcca tccagttcta accgcgattt gatcaggact attatcttct
     3121 ttaactcgaa ttcttccatc tttaatctca acaatattat attgtccctt cgcaggatga
     3181 taattcacga gtttatgatt gatcttatcc aaatcgaagc gatccatttc cttgccatca
     3241 attcgaacga tggcatattt gatagcatct tcagttgttt cattctcctt ttctatagag
     3301 aatacaaata agggaataaa agaggtaata atcaaaatgc caataattat gacatcgcct
     3361 atcttgacac cagcttttct taaaaatttc attaacgata tctggctaaa catattagac
     3421 tttttccatg gaaatggtga aatttttcgg aaattatctg cattaagaga atcagttaaa
     3481 caaccctcaa gttgttcctt aatacattgt tgatccatct tcataccaat aaaaactagt
     3541 ttattaatgc gttcaccaaa caacggatgc caggcttcct tcaattcggg atttttttgt
     3601 ataatcattt gacactcaat tttaggtaaa ctagctaccc aataatttaa tgattcaata
     3661 cttttatagc tacccgatgt ttcaaagatc gtcgaaaaat cactatgttg ggcaaaccaa
     3721 agaaaccctt ttgctcttgt aatattgcca ggaaaatttt tatccgtaag atgggcaaat
     3781 ttttgtgcgt caaggagtaa ccgatttcga tagataaaac ttgagatacc atattcttct
     3841 gtttcaggaa tatgattttg atagccaagc tctaactctt tgaaccagcc cactgaatag
     3901 gaagtctcat ctatattaaa aagatttgta tttagaattt tcgataagtc tacttcacct
     3961 ttaattgtcc taattatttt ggcattggac tgtaacttag caattagacc gttaattttg
     4021 taaatcgtct tggttgacac taagtcacta ttatttaaaa gtaatatgtt acaacactcg
     4081 aattgatcaa ttaacaagcc acgaatatcc tgttcttctt caactacatt accttcagct
     4141 tttctttcct gaatcttttc acccgtttcg aaattatccc aaaaacgttt tacatccact
     4201 actataataa tggcaccaat ctctaatcgt tgggtcaaat caatgtctaa agggttttta
     4261 gctaatataa ttgcttgaat aattggaatt ggtttgctga ttcctgaagc ttcaataaca
     4321 atttgattca agtcagaaaa ttgcactaat tgatctagtt caacaatcaa atcttcgcgt
     4381 aatgtacaac aaatgcatcc attcggcatc gaaacaagtt tttctccagt tcttttaaaa
     4441 cctttcttgt cgactaatac cccatcaaca tttacttccc ctatgtcatt tacaatgaca
     4501 gcaattttct ttccgtgatc accgtacaga atatgattta gaagtatcgt ttttcctgaa
     4561 ccaagatagc cagttaagat tgtaataggt atttttttca tagggaacct ctttctatac
     4621 cttgaattta cactttaagt gcaacacata aaaatgagtc ataatcgata tccaactagt
     4681 ataagtttgt cgagaacact cctagaagga aaataattat gagccagtat cgtcatctta
     4741 ctatactcga acgagaaaga atctttctgc ttcacgaaga aggattttct attagtataa
     4801 ttggcaacga cattcagcat agcccgttta ctattttaca tgaactgaga agaaacaaga
     4861 aaaaaactat tcaccttcca aagcggaaaa aaattatgct tatcgaagaa aaaaatgcgg
     4921 acgttatcac attctaaatg atccgtgagt ttggtcaatt gttcgtcatt tatttgtcga
     4981 ttttcagtgg tcgccagagg aaatctcgaa tcgtttgaag catgaacata tggacttttc
     5041 aattagtttt atcacgatct aacgtgcaat ttgcaatgat cttttcgata cagaacccct
     5101 ctcacacggc aataaaggcc tgatccgttc attgcaccat cgaggaaaaa caagacatac
     5161 aaaaaatcat gtcgagtctc gtggaaagat ttgccttact cattcgatcc atggccgtcc
     5221 aaaatccgca gatgatagaa gtgattttgg tcattggaaa gccgatattg taactggcaa
     5281 aactggtggt acttgtttag tgactttgac agacagaaaa tcatgcttcc ttttagcagg
     5341 aaagaaggtc cccagaaaga aagccgctta tgtgagcgag aaaatgattg aacttttaag
     5401 cagtattcca aatgaggcgg tcaaaacgat cactccagat agaggaaaag aattctccaa
     5461 tcatttcgca gttacagaag cattgaataa tatgcccttt tattttccag atcctcatgc
     5521 ccttggcaac aatgaacaaa ttaaaatacg aatagactca ttcgagaata cttgccaaaa
     5581 tcgaaagaga tagctacggt agataactca acgataaatc agatgattga aaaactgaat
     5641 aacagaccta gaaagtgttt agattggaga acaccttatg aagtcttctt tgataaagtg
     5701 ttgcgcttga tttgacaatt caagacataa taaaaagcag atcaaatcca aataagggaa
     5761 aaatgatcta ctctggtatt atcaattaat tggccaagct attatgccgt tttgctaaac
     5821 gtctacttct tccagtattg accttgataa aacaacaatc aggatcccgt tttaatactc
     5881 tatgaactgt gtcaatacta acatgtaaag ttgcagcaat atcagctaat gtactcccat
     5941 ttaatctcat ttgctttgca aatcgataac gccgataagc atcaatattc ttggaatcca
     6001 tagacaataa tacttttctt accatgtatt tcctccaaaa attgaatttt aatgagccat
     6061 catttcttga gcaatttcat gtttagacaa tgaagctgga tagaaagtag gccaattatg
     6121 caattcatta tagagttttt cttgactttc tccaccaaag aaaatatgga aatgctctgc
     6181 cttacttggt gcaattccat gatcactaaa ttgaatatat ttaaaacgac cagcacctgc
     6241 atcatcagtt tcaaaattaa aacgaacacc acggtttcct ttttcataat tcaaaatttt
     6301 gtatccaaca tatttgtatg tgtattgatg atgttcaccg ttgactacaa aatcaatggt
     6361 gttgtcttta atgttgatag tatcaatatc tgtcttatag ccagtagtat aataatcttt
     6421 gtactcggca gctgtcatgt ctttattgat ttttgctttg taatcgaaaa cttcatccaa
     6481 aattccatct tttaacaacg gataaacaga ttgccattcg cctgtataat cactcaattc
     6541 gcgatccttc acatcggcat ctttaaagta cccattagca actgtctttt catccttcgc
     6601 ttctttttct ggaactacct cttcaccagc aaccatagtt gttttttcta acgcttttag
     6661 attgtcttcc attactgaaa cataatcttc accattatcc atttgttctt ttgtaagact
     6721 ttcaagagga ttcagtactt ccaattttat cccagcttcg ttggccaaag tttttgcaat
     6781 tttatcatta gcattctttt caaagtaaat ataattgatc ttattttttt taacatactc
     6841 ttttaattca gctaatcgac cagaagatgg ctcttcttca ggacttagtc cagcaatagg
     6901 aacttgtttc aatccataat ccaaagctaa atatccaaag gctgcgtgtt gcgtcacaaa
     6961 acttttttgt ttcgcttctt taagactagt ggtatagtca gcatccaacc gtttcagttt
     7021 agtcaaatac ttttcagcat ttgtctcaaa tacttttgct tttttcggat acaatttaac
     7081 taactgatcc ttgatatttg aaacttcctt aattgccatt ttgggtgaaa cccacgtatg
     7141 aggatctagt tcatgatggt gatcttcttc tccgtgatca tgatcttctt cttcactacc
     7201 tggcaataaa atcatgtctt ttgttccttc tactacatta ggcccagctt tcttccaact
     7261 ttctttagct tttggtaccc atgattccat gttttcgtta tgatatacaa aaacatcagc
     7321 atcatgagct tttgccatat ccttagctga gggctcatag tcatgaggtt ctgatcccgc
     7381 tggaaccatt aaatccacgt tcgcttcatc gccgacgatg tttttggtga aatcatacat
     7441 tggatagaat gttgttacaa tttctaattt tccatcttta tcattcgata cttttgcatc
     7501 attagtgctg gagcaagctc ccaataatcc tagagctgct atcccaataa gtcctacact
     7561 gattttcttc attctttttc cttccttaat tatgctaatt tcttcatttt atttcgttta
     7621 tgttctttga taatttttaa tgcccttttt ctagttttaa tattcgggct tttaagtcgt
     7681 cgatatgcac tagatatatc cgttttctcc attagaaatt acccccaatc actacttatc
     7741 attatttgct ttccgccagt aatttatcaa aactctgttt gatcctttct tcatccaaca
     7801 tttttccgat tagaataata gtggtttggt ctttttgtcg agtggtgtat tgaaaattaa
     7861 cctgttcgtt tacctcttgc atggcaacta taaaatcctg ctcttgtaaa gcaatcaatc
     7921 ctttaaaacg atacaggctc ccttggttgg ttaaaatcag ccagtcaatc cattggagta
     7981 aaaaatcttt ttttatgtct tgatctgaag ttaagagaat tgatttaaac tcatgtactt
     8041 gatggtgttg atgatagtat ttgccattat agtgattttc tgatgaatca ctttcaaaaa
     8101 ctaagggatg attaaatttt ttcaaattat aaaaatcctt tttttgaatc gtttcactat
     8161 gtgtgaacat tcgaatatcg gataaagggt taatactttt gatttctctc tgtaattttt
     8221 ttagtgcaga taaattcaag ttttgttttc tagacacaaa aatgcgatct gccatgacaa
     8281 gttgcttaag ggcttgtggt tctctttcaa taagttcaaa actgtcacta tcaataacag
     8341 tcaataaact atcgatatat actcttcctc ttagttgagg cgctgttaaa agtgtttgaa
     8401 tgattggttg gggatctgct atacctgtag tttcaatgat gatttgtgaa attggttgtt
     8461 gatgttcttc tattgtttcc agtaaagaaa tcaatattgc taataaatca ctgcgaagac
     8521 tgcaacacat acatccatta tttagttgta acacccgttc ctcgctatga actaataatt
     8581 tgtgatcaat cccaacttca ccaaactcat tttcaattat aataatttct tccctggggt
     8641 agggtgagac ttgcaaggct tgattgatta gcgttgtttt tccagcccct aaaaacccag
     8701 aaacgattgt aactggtatg cccattttgt caccttttta ccacttagct atttcacttc
     8761 tttaaaaatt gcccgtctgc gcaattttgg cgaatacttt tttaattcta accgttcagg
     8821 gttattccgt ttatttttgc tggtgagata cagacgttct ccagtttcga cacattccaa
     8881 aataatattt tgacgcataa atcttcctcc ttcaatactt atcgatcttc aagatatgct
     8941 tgaatttgtt gtgcatcttc aaaagttaac tcgccagatt ccaattgtga aactaaattt
     9001 tctttcatat caacaggtat ctcccaataa ccatcgatac attgaaataa ctcctcgtca
     9061 tactcttcaa aaattggata gattcttaaa aaatcatcaa atgataagcc ttggtcctca
     9121 tagtaatctt tgactaatcc attttcaatt gcctgttggg tcggtaaacc cgtttcagta
     9181 ataattcctc tctctaccat cgaacgataa aaagtgcatt tatctagcga ttgctgggtc
     9241 gtttcaagat aatactttaa tccttcttca actaaaggtg ggtaaataat ttccattttg
     9301 attccctcct aaaagttata tattaaacta ttttgaagct cctttaatta ctttctttcc
     9361 attatagtac ccttttaaag atacatgatg gctacgtcta taatcaccat tcgcttcgtc
     9421 aaatgaaatt tctggtttta ctacagtttg atgagttctt cttaaccgtt tcttcgcctt
     9481 agatgttttt cttgctggta ctgccatgat atttcctcct tttaccaact tgcttttttt
     9541 actccaggaa tcaacccttg atgggcaagt tctctaaact taatacggga cataccgaac
     9601 tttcgcatat accctcttgg acgaccatcc gtctgatcac gaagcctcag acgattcgga
     9661 tttgaatctc ttggcaattt agcgagagcg gtgcgatctc ctgcggcttt taattcctga
     9721 cgtttatctg catacttttc aatcatcgcc atttgtttct ttgctttagc aattttcgac
     9781 ttttttgcca tataccttta tactcctttt attttgattt agacacctca aatcgtaatc
     9841 gttacgtttt gacactgata ctttaccaac aagagttgct cttgtcaaca ataaataaca
     9901 tgaaataaaa ctcatatgaa tagataacta tttgaaaacg aatttatcgg tacatagaga
     9961 taatcaaatc atttaaaaga atatgcagtt taggctctga aaaataagaa ttcactttta
    10021 ttagtccaat caaacacaaa taccgtttta agtagctttt cctctactta aaacggcaat
    10081 atgaaagaaa ctataacttt ttattcaatc ttttatgttt aactggttta tcgccaattt
    10141 tttaaaactt tgattattcc ttatattata agaatagaca ttaagtgaac taattcaatc
    10201 atactgattc tttcgtgtat tcaaaattag atgctgttta ataaaattat tctttttgaa
    10261 aaagaattat aactatatcc caaaaattgt aggttaattt aaagcctatt tcatcatcgt
    10321 gattgagctt ctttttcata gacgaatttc tcagatagtt tagttttctt tatgagattt
    10381 ttgtatattt ctgaccttcg atacaactca ttatgagtac cttgatcaac tagttttcct
    10441 tgatccatca ctacaatttt atttaccttt tcaatagact tcaagcgatg cgatataatt
    10501 agtactgtct tatcttgtat taaacgattt aaactttcct gaatacgttt ttcattttcc
    10561 gcatctaaac tggcagtaat ttcatctaaa attaaaattg gcgcattttt caaaaaagcc
    10621 cgtgctatgg ataggcgttg tcgctctcct ccagaaagtg aacttccgtt ttctccgacc
    10681 aatgtatgat aaccgtcagg tagtttttca ataaagtctt cgcagttagc taaacgagct
    10741 gcttttttca cttcctcatc tgacgcttga ctatttccaa tacgtatatt ttccaaaata
    10801 cttgtattaa acaaagttac ctcttgaaaa acaatagcga ttttgctaaa caaagaagct
    10861 ggagatacac gtttaatatc atacccatca attaaaatac agccttcatc ataatcatac
    10921 agtctagaca ctaatttcaa gatactagtt ttcccggaac cactagcacc tactaacgca
    10981 gtcacttctc cttgtttagc cgtaaaagag atatgatcta aaattggggt attgttatca
    11041 tatgagaaag agacatctcg caactcaatg tcaaaagatt tcagaggact atctgatccc
    11101 tcttggatac tggtttcctt catagctcta attcgctgta ttttaggagc taaataaaat
    11161 atttcaagca ctgcttcttt catagaatcg aaggaatcct ttatttttat tgcggctagt
    11221 aagtaaccaa cgacgtagag tatagtcact tcacccgtta acagtaagtg aacacctact
    11281 agaatgacta ctgctaaact aacatatgag aaaatagagg ataaggccat gactgaaaaa
    11341 gtacttaatt ctacctttaa atgaatcctt tcactctctt ccattttttt gaaaagtctg
    11401 tcttgaactt ttttagatag attaaagcta ttaatctctt gctgcaattc tatagtttct
    11461 tgaaattctt ccgagttctc tctcaaagta tcgtaatatt tagtattggc tttcgtttgg
    11521 gatttctttg ataacaagat taagacaaaa ctaaataacg ttggcaaaat aacagctaat
    11581 cccattttga cattaccaac caaaagcatc actgaaataa aagggaaaaa cagagccata
    11641 ccgccggctt taggtattgc atgactcatc gcatgctcaa taccttcaac gtcagacatg
    11701 atagtttgag ataaatctga taaattatgt tttgaaaaat aggataatgg taaatttgac
    11761 aaatcgtctg caatttgcac tcttaaatgc gcactttctt tataagtact attatataag
    11821 ttttcatact ccctatctaa caaccaatac atgaaaagca aagtaacaaa cgatacggca
    11881 aaataaagcc aacggggctt tgtattttct aaaactaatt cgtctatcag catcattata
    11941 atgaatgctg gtaccatatt aatgcagtat acaaaaaaac tagaaataga agctttagtt
    12001 aaagcttctg cgccctgatc acttaaagca aatttgtttt tataaaaact tttcattgtc
    12061 aagcctccaa tcatttgcat tctcataatt agtgaccaat tgtttataca cttgatgttt
    12121 agccattaat tcattatgat ttccacgttc tactattttc ccttctgaca agacaagaat
    12181 ttcatctaca gatttaatgc tggataatcg atgagctatc ataataactg ttttttcttt
    12241 catcagatgt tgaaaagcct tttgtagttc atactcatta tctggatcaa tagatgcact
    12301 ggcttcatcc ataataacaa tctttgcatc ttttaaaatt gctctggcaa tagctatacg
    12361 ttgtttttct cctcctgaca gataaactcc tttactacca attaaggtat gctctctttc
    12421 gggaaatttt tctaaaatcg attcgcaacc ggctaatctt agcgcgtcaa atactttact
    12481 tgctggcgct tgaggatttg ctatagaaac gttatcgtaa atactctgat tgaatagttt
    12541 cgtatcttga aagacaaaag caattgtgtt aataattgtg tcttttgaat agtgatttaa
    12601 aggcttacca ccaattttaa tctgtccctt atccacattg tagaaacctg aaattagttt
    12661 agctatcgta ctctttccgc ttcctgattt tcctactaat gcataaattc ttttttgttc
    12721 taaaactaag ttgaaatttt ctaaaacaca ctcttctttg tatgaaaagc tgacattttc
    12781 aaatgcaatg tcacaatttt taatctgtgt ctcgttccca tattttagtt ggtcttgctt
    12841 catttctaaa taaagggagt ctagtgtttc gactgcatat cttgcttcaa acaagtgcat
    12901 acttacatac attattctca tgaaagaaac aaaaataatt ccacttaaaa aaaacaacat
    12961 caacaagtca acagctaata tcttgggagt ggtaagagaa gttataaaaa aggttatagg
    13021 aactatcaaa atgcaaacta tgccaaaaaa tagccattga aaaagaacaa aaggtgtttt
    13081 cccactcaaa gaatacttat atgcaaactt agcataatcg ttaattgctt tattcaaggc
    13141 tttaaatgat gttactttag caccaaatat ctttataact tgaattccgc ggatatattc
    13201 taccgtttca gagcttaaca catctaacga ttcttgatat ttttgaataa aatttgtatt
    13261 ctctgtcatt ttctttaaca agaatacacc aattactaac atggcgatta gaaccaatcc
    13321 aattttaaag ttaatgaaaa agcctaaggc aactgataaa acaggtgtta ctatggcttg
    13381 agcactatct ggaatcaagt gagcaattgc catatgtgtt ttagccgcgt tgtcatcaat
    13441 tgtctttcga atatatcccg aagaatttaa atcaaaaaaa cgaaaacttg cttctgttaa
    13501 accgctaatt cccttttttc tcaaattagt ctctaagcga aacccaagga tatgagaaaa
    13561 taagccagaa aaaatataaa ataaagctcc taaagttaac caagcaacag tttgaaccgc
    13621 ataactctta gctaaatatt catctcctac aataattaga gactgaagaa atttgtaaat
    13681 actgtaatat ccatagactg ttaatacgac agaaaaatca gaaaataaag tggctaacca
    13741 tccgtagtat ttcattttag ggacataact aaataatttt ttgtatactt tcattttttc
    13801 ctcccccgca ttcaatttct tctcttattc gtttacaact gttaatgctt tccgtaatag
    13861 cgtaaattag agatgtacaa acagataaaa ggtaaactac gttctatttt ttgtattttt
    13921 ttaagacttt tcttccaatc aatacaccaa tcatagataa acttgctaca actaacactg
    13981 aaatgattcc ccatatacct accgcatagt ggatcattgc atccattgtt gcagcagatt
    14041 gtcctttatc tatccaatct tgacgaaaac gatctgtaaa gaaccatata ggaatccact
    14101 gtccggcagc actgcctaat tgcattagag cccaagacgc aacttgagga attgctcgat
    14161 catatccaaa tacagataga ataatatcag ctaagatcgc catactaatc gaactgataa
    14221 accaaggtaa gtacgccccg cccattaacg taaaaaataa caataaaaca attgtgtaaa
    14281 tagtaaaaat tccttttcta gggtagttat agctaatata aacaaatatg attccagata
    14341 ataatccaaa aatcccgggg gatatgctgt gaccaaacgc cccaccaata ctactaacca
    14401 ttattgaaaa aaagtaaatt actagggaaa taactgtagc taaaccaata aaaatataat
    14461 cttttgtttt catatatatt ctcctcaatt ctatttcgtt atgtcattaa aaatctgttc
    14521 tatcaaagtt gaatcttcta ctctaagttt tatatctttt tttattccac cattttttaa
    14581 ataaataatt ctattgcatg ttttataaag aaattccgga tcatgagtag ctataactac
    14641 tgcacccttt tcttttaagt ctttaataag gtcagcaatc ctttccatat taatataatc
    14701 caacccactt gtaggttcat caaatagaaa taaattagtt tttgacaatg aagctataga
    14761 aaccaataat ctttgttttt ctccaccgga taaatcaaac ggtaattgat ctttcatttt
    14821 ataaagttcc atttttttta aaattttttc ttgctgatct ttactaacta tttcattccc
    14881 cagggctaac tcgctcctca ctgattctgt gaaaaattgg taatccattt cttgcattac
    14941 taaaaacggc gatgacaaat tttttttatt ttcgattatc tttcctttgg tagggcgaat
    15001 aatcttcatg agtattctta gtaaggttgt tttccccaca ccgtttttcc ctaccaaacc
    15061 aataacatct ccaactttga catcaaaatt tatgtctttt agaatatttt cataagtaat
    15121 ttcttcaact tttaatacat ctgcacttac cttttcgata tagggaactt caatcgaact
    15181 attaaaaatg tcaaatttcc ttaatgcgtt attgtttt
//
LOCUS       DAZBSA010000067        14681 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000067 DAZBSA010000000
VERSION     DAZBSA010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.067"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            146..1042
                     /locus_tag="ACHGMT_001932"
     CDS             146..1042
                     /locus_tag="ACHGMT_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFN6835667.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1192..2133)
                     /locus_tag="ACHGMT_001933"
     CDS             complement(1192..2133)
                     /locus_tag="ACHGMT_001933"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFN6835668.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2259..3380
                     /locus_tag="ACHGMT_001934"
     CDS             2259..3380
                     /locus_tag="ACHGMT_001934"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFN6835669.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3638..5140
                     /gene="eat(A)"
                     /locus_tag="ACHGMT_001935"
     CDS             3638..5140
                     /gene="eat(A)"
                     /locus_tag="ACHGMT_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFN6835670.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5207..5734
                     /locus_tag="ACHGMT_001936"
     CDS             5207..5734
                     /locus_tag="ACHGMT_001936"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFN6835671.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5945..7006
                     /locus_tag="ACHGMT_001937"
     CDS             5945..7006
                     /locus_tag="ACHGMT_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFN6835672.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            7009..7905
                     /gene="murQ"
                     /locus_tag="ACHGMT_001938"
     CDS             7009..7905
                     /gene="murQ"
                     /locus_tag="ACHGMT_001938"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFN6835673.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQVKLAEANGFVKGTL"
     gene            7919..9901
                     /locus_tag="ACHGMT_001939"
     CDS             7919..9901
                     /locus_tag="ACHGMT_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFN6835674.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9966..10412)
                     /gene="nrdI"
                     /locus_tag="ACHGMT_001940"
     CDS             complement(9966..10412)
                     /gene="nrdI"
                     /locus_tag="ACHGMT_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFN6835675.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            10623..12047
                     /locus_tag="ACHGMT_001941"
     CDS             10623..12047
                     /locus_tag="ACHGMT_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFN6835676.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(12228..13001)
                     /locus_tag="ACHGMT_001942"
     CDS             complement(12228..13001)
                     /locus_tag="ACHGMT_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFN6835677.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(12998..13903)
                     /locus_tag="ACHGMT_001943"
     CDS             complement(12998..13903)
                     /locus_tag="ACHGMT_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6835678.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(14057..14377)
                     /locus_tag="ACHGMT_001944"
     CDS             complement(14057..14377)
                     /locus_tag="ACHGMT_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFN6835679.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         4693 a         2612 c         3102 g         4274 t
ORIGIN      
        1 aattttaata gaagaatgat ttatttcatc atattttcat acaaatgtta aattttcata
       61 caaatatagg aaacgtttta ctggcttttt actatttaaa caggctatca taggaaaaag
      121 tgacagagaa agtgaggcgg aaaagatgaa caaaaatgac gtactgctag ttgcaaaaaa
      181 taaatcactt tattttttgc cttccatccg ctctttagat gtttggtccg gaaaagattt
      241 actacagtcg gaattcatta aatcagctag acaacagttg gcaaattatc agctgatcgt
      301 ttttttagat tatggatttg atatggagat ggctcgacgc gtgcgcccat ttaccaatgc
      361 aaaaattgtg ttattttttt ggaatcactt caaagcagaa cacgaggcga tgctgaagca
      421 ggcacaagat gaaccggcaa tcgatgagat ttatcatttt gattttttgg aagcaaaaga
      481 acttgggctg aaacataata gttcatttta ttcgaaacat atgaattttt cttctagcga
      541 accaaagacg gatctatttt ttggtgcaac gaataatggg agaaaagaac gagcagaaag
      601 ctataagcag gaatttataa aaagagatgt tacgaccaac tatttcattc ttccacatcg
      661 agggaatgaa caacctggat atcttactta tgaagagtac ctgaaaaaaa caggggaatc
      721 gcgcgggatt ctggaactgt tgcgggaggg tcagcaagga gtgactttgc ggacatttga
      781 atccatgtat tttcaaaaga aactagtgac ggataatcaa gcgattgctc attatcattt
      841 ttatcgtcct gaaaatattt ttttgcttcg agaacgtgat ttagatgaat taccggaatt
      901 tctgcataca ccatttaagc caatcctatc agagctattg gaattttatg atgcagaaaa
      961 ttgggcaaaa cgatttatca ctaacgattc cactatttat gagcaatacg aatatgacct
     1021 ctcctctgtt gaaaacaaat aaaaaaccta cattttgaag agattatgta ctgaaacaaa
     1081 caagcacaat tcttatggca tcatgagagt tgtgttttct ttttgatctt ttattgggct
     1141 taaaaaaaga gaaccagctg ctgattcagc tgattctcat cagagagcct tttattctgc
     1201 gccaaggatt ccttcttttg catggactaa ttgggtcaaa aaggcacaat atggtgtagc
     1261 aatatcatat ttttgtcctt ttcgccaaat ggcaccattg atgtagtcga tttcagtcaa
     1321 acgatggttt ttgatcaggt cttggtacat agatgggtaa tgcaacccga tgttgtcagg
     1381 atcataagta gaagcaatat gttggtacac ttcttcttga tctagtatga ttccttcttt
     1441 tgctgcgata gaagcaaatt cagatacgat tgttcgaacc atgctctctg ccgctttttg
     1501 cgcgcctagt tcagcaatat tacaatccaa aatcgtgcat aaaccattca atgttccatt
     1561 tacacaagct tttcgccaaa tggaataacg aacgttatgg ctatagatcg gatttaaatt
     1621 tgcttcttga aagacttcta caactttttt tgtaaatgct tcgccttctg gttccaagtt
     1681 ctctagttcg atttctccat cacctaacaa tgtgactttt ccgggaccag ctagaccagc
     1741 tgtccacatc gtgatcccaa ataagatatt tttctttggc acatattttt ccagtacatc
     1801 ttcatgacct aatccattta gtaaacacaa aacataagta ttgtcagtaa taatggattg
     1861 gatcgaacaa aacatatcat ctaattgatt ggcttttgtt aatgctacga tcaagtccac
     1921 gtgttcgttg ctttcgatga tttcttctgg tgaatagatt ggcagttttg cgaccacttc
     1981 tttaccgtta aagtcagcaa tcaacccatt tgtacgaatg gcttcaatgt gagcaggcca
     2041 acgatcgatc aagaggacct catttccgct ttgatgtagc atcaatccga ttcggctgcc
     2101 cattgctccg gccccagcta ttgcgatttt catgtttttt ctctccttta ttcaatgagt
     2161 tcccttttag tatatagaaa aatggaagaa aatagcattg ttttgtttga aaaaaatgaa
     2221 aaaaagtgtc ttacttaaga agaaagtgag ggatacagat ggatattttg gcggcaattg
     2281 attcatttaa aggaagtgct tcttcatttg aactaaatca ggcagttcta aatggctggg
     2341 aagatcgtaa aggaagaaaa atcaatgtgc cgattgcaga tggcggagaa aatactgcag
     2401 aagcaatcta ttacgcgctc aggggaacat ggcgtaccat tgatgggatg gatctcttat
     2461 tccgtcagcg aaaaatcagc tatttattga caacttggca aggaaaaaac accgcagtca
     2521 tcgaatctgc acaagcattg gggttagatc tacttgataa accaacggat caaactatcc
     2581 gacttgcttc cagttatgcc ttaggagaac ttattaaaga tgcactgctt caagaagtag
     2641 aacaaattat tgtcacttta ggaggaagcg ggtgtagcga tggtggtcta ggattactcc
     2701 aatccttggg tgcttcttta gaagggtaca cagaaggaaa tcctttatta tctactaaaa
     2761 aacttcattt tgatcgaaga aacgttcttg tttctaaagt aaaattgatt atcgctgcag
     2821 atgttaccaa tccatataca ggtcaacaag gagctgcttg gcaatttgga agacaaaaag
     2881 gaggaataga tacgacgcta gctttcttag ataaacaagc aggaaagatt gctcagcaaa
     2941 taaaagattt ctgggaggtg gatttagatg atattccagg aagtggagca gcaggcggta
     3001 ttggtggagc cctttttttg ttgggagcga agatggtttc tggtttcgat ttagtcagcc
     3061 aaatcactgg attggaaaaa aagatcgaac aagcagatgt catcattaca ggagaagggc
     3121 ggatcgatca gcaaacgatc cacggaaaag ttccatacgg tgtagcagtg cttgcaaaaa
     3181 aatatcgaaa accaataatc gctatttgcg gaagtagaga ccaagagcta gaaggacttt
     3241 ctgattttct cccaattgtc ttaagtattc atttgggtcc aataggttta gaagaagcaa
     3301 tggaacacag gcaaacatta gaaaaaacaa aaatcttagg acagacactt agcagatttt
     3361 tcaccatcct cccgaagtag ttgacaaaat cattttcttt atgctatcgt aatttcaaat
     3421 ataggacaaa gtgagggatc cgtgcattga aaaattaagt cgattgatga aagctgcatt
     3481 cagactgtac cttgtggaga caaggaagca gtgtgcagat tttcgggatt ggcttggttt
     3541 tttcgatgta tgggtctttt tgtgtgtatc caacccttgc atcctcttta gccagttctg
     3601 aaaataggac tggtcttttt ttacgaggtg atggaaaatg tctaaaatcg aaataaaaaa
     3661 tctgacattc ggctacgaca gccaaggcac attattattt gaacaagcaa atctaaattt
     3721 tgacacacaa tggaaactag gacttatcgg acgaaacggt cgaggaaaga caactttact
     3781 gaatattcta caaaacaaac taccttatca agggcaagta atccatcagc aagaatttgc
     3841 ctatttcccg caacagacaa aagataaaga acgtttaacc tattacgtgt taaatgatat
     3901 tacggatttt gagatatggg aaatcgaaag agagctccaa ttgatgcaaa cagatcctga
     3961 aatcttatgg agagaattca gcacactatc ggggggagag aagacaaaag tcctactggc
     4021 acttttattt gtggatgaca ctcatttccc gttaatcgat gaaccaacga atcatttgga
     4081 tatctctggt agaaaacaag tagcggctta tttgaaaaag aaaaaacaag gcttcatcgt
     4141 ggtcagccat gaccggggat ttatcgatga agtagtggac catgttttag caatcgaaaa
     4201 aagtcaactg gaactttatc aagggaattt ctctatctat gaagaacaga aaaaacttcg
     4261 tgatgaattt gaaatggctc aaaatgaaaa attgaaaaaa gaagtcagta ggctaaagaa
     4321 aacagcagct gaaaaagccg aatggtctcg ttcccgagaa ggagataaaa caaagaaaca
     4381 agtcggattc atcgatactg aatctagacg agtgaataaa ggagcagtgg gtgctgatgc
     4441 tgcacggacg atgaaacgat ccaaagcaat cgtgaatcgg atggagaccc agatcagcga
     4501 gaaagaaaaa ctattaaaag atatcgaata tatcgattct ttgacgatga atagccaagc
     4561 gtctcaccat aagcgacttt taagcgtaga agatcttcaa ttagggtatg aaaatctgtt
     4621 attcgagcca attcatttta caatcgagcc tcatcagcgg gtggcgattt caggtcctaa
     4681 cggtgcagga aagtcatcca ttatccatta tcttctgggg gcattcaacg gcaaggttat
     4741 aggagaaaaa taccagccaa aacatctgag cattagttat gcaagccaaa attatgaaga
     4801 caatcgagga acgttggcgg aatttgcaga gaaaaaccaa gtagactacc aagcattttt
     4861 gaacaacctc cgaaagcttg ggatggaaag agatgttttt cataacaaga tcgagcagat
     4921 gagtatgggc caacggaaaa aagtggaatt ggctaaatct ttatcacagc cagctgaact
     4981 atatacatgg gatgaaccat tgaattattt ggatgtcttc aatcaagaac aattagaaca
     5041 actgatcttg aacgtgaaac ctgccatgtt actagtggaa catgatcaaa ccttcctgga
     5101 taaagtatct actgagatta tttctcttga gagaatctaa cccttgtaat ctcaagaaca
     5161 acaaggtatg gttaagggga actttgccaa cgaaggagag aatcacatgc tttatgtaac
     5221 gagacacgga gaaacaactt ggaacgcaca aggattagtt tgcggacggg cagatgtgcc
     5281 gctgactgaa aaaggacaga tgcaggcaca gaaactagca gaaaaggtag tagatttgcc
     5341 tgttccaatc acgaaaatca tccattcccc tcttcaacga gcaagagata cagcacaagc
     5401 agttgctgat cgtctcagct tgccgctgac cgttgatgaa cgattggtag aaatggattt
     5461 tggggattat gatggactgc caagcaaaga tgagaacttt caaaaagcac gtttagcgtt
     5521 cgctgtacga ttcccaaatg gggagtcagt cttagatgtg tatgcacgga tcgttccttt
     5581 attgaaagaa tgcatcgaag acgaagaaaa tgtctatctg ttagtttgcc ataatgcctt
     5641 gataagagtg atcaatgctt attttcatcc gatgcctaat gaggggttct ttacattcat
     5701 ggtagataat acagaattgg tttcttatga gtaattatgt ttttgtgtta tttaaataaa
     5761 atatttcaag gcactgcttt tgcttagcag tgcctttttt aggttatgac aattcttttg
     5821 ataatataaa ctcaaccttt agaaatgaaa tttaattatt ttaataaaag tattgaaatt
     5881 ttgtttcttt agatatatac tctagtcata gaaatgaaga cggatacaga aaaagaggga
     5941 taatatgttt ggcttttcaa tatttatgaa cgattctttg tcagaagaga agaaacagta
     6001 tatccgccaa atggcatcca atggctttgt cggtattttc acttccatgc atataccaga
     6061 agatgatgtt tcggcatatc aaaagcgatt gatcgaacta ggaagcgaag cgaaaaagaa
     6121 taaactagaa ttgatggtcg atatttcagg agaggcattg gaacgagcgg gtttttcatt
     6181 agaagatcta acgcctttga aagcaattgg tgtaacaggt ttaagaatgg attatcatat
     6241 ttccaatcag cagattgccg actggtcaca tcaattgaaa atcagtttga atgctagcac
     6301 gatcacgcct aaagatatcg atgaattgaa agaagcagaa gctgatttct cacagattga
     6361 agcttggcat aattattatc cacgtcccga aacagggctg gacaaagaat ggtatcagaa
     6421 aaaaaatcaa tggctcaaaa ttcaaggtct ccttgtccaa ggttttgtac caggagatac
     6481 agagttgaga ggcccactat atcaaggatt gccaactttg gaagaacatc gagggatcca
     6541 tcctcttgca ggcgctctag atctattagc aagtaacacg gatattgttt acatcggaga
     6601 tgcaggtctg tcagaaaacg ttcaggagca atttgcttct ttccaaaaag aacagacagt
     6661 cttgctccat acggaacctg tagatgaaga attttacgaa tatatattag ggaagcatac
     6721 gaatcggcaa gacgatgcaa gagatgtcat acgaagcgta gatgcacgtt ttcgagagat
     6781 tccgcctatc ccagcaagaa atacagctac gcgtatgaaa gggtctatta ctttagataa
     6841 cgaaaagtat ttgcgctata tgggtgagat ccaattaacc aaatatgatt tgccagcgga
     6901 tgaaaaagtg aatgtagtag ctaaagttat caaagaagag ctaccattga tcaaccaaat
     6961 caaagctggt atgaactatc aatttataag aaaagaagga agataaaaat gagcaaaatc
     7021 aatttagaga acctaacaac ggaacgcaga aaccaagaaa cttttggatt agatgagatg
     7081 agtatttctc aagccgttca actaatgaac aaagaagatc agaaagtggc cgctgcggtt
     7141 gaaaaagaac tgacaagtat cgaaccagtg attgctaaca cgattgagtc ctttaaaaaa
     7201 ggtggacgtc tgatttatat gggagccgga acaagtggaa gactaggtgt gttagatgct
     7261 gctgaatgcg taccgacatt cggtgtagaa ccggaaatgg tcataggtct aattgctggt
     7321 ggagaagaag caatgactgt agctgtcgaa ggggccgaag attcttatga gctagggcaa
     7381 gaagatctaa tcaagctgaa tttgacaaaa aatgatatag ttattggtat cgcagctagc
     7441 ggtcgtacac catatgtgat cggtggatta gattacgcaa aatcgatcgg tgctgtcaca
     7501 ggaacgattt cttgcaataa acaagccgaa atcagtaaac acgcggattt cccaatcgaa
     7561 gtcgatgcag gtccagaatt tttgacaggc tctactcgat tgaagtcagg aacagcacaa
     7621 aaacttatct tgaatatgat ttctactatc agtatgattg gtataggtaa agtattcaat
     7681 aacttgatgg tcgatgtcaa accaacaaat gaaaaactaa tcgaacgctc aaaacgaatc
     7741 atcatgcaag cgacagaagc tgattacgaa acagctgaaa agtactttga agaagcagat
     7801 caaaatgtta agctggctat cgtcatgatt ttgacaaata gtgataaaga ggaagctcaa
     7861 gtaaaattag ctgaagcaaa cggatttgta aaagggacct tatgaggggg aaaagaccat
     7921 ggcaaaaaac gaactgactt tggaagaact ggcaaaacaa atttataaag gggcaggcgg
     7981 catgggcaat gtagaaagca ttgtccactg catgactcgt gtgcgtatga agatcatcaa
     8041 tgatgatctt gtaaatgtct ctgaattaaa agcgattcca ggagtgcttg gtgtagtaga
     8101 agatgaacag cttcaagtca tcatcggacc tgggaaagtg aacaaagtag cacagcaaat
     8161 ggttgatttg gcaggcgtga aattaggaga aaaactttct tctgtaagta cagctacagc
     8221 aaattcatca gcatctggaa aagaccgtgt aaacgaacgg gcacaagaaa tgaaagcggc
     8281 acaaaaatcg aaacaaaaac catctaaaat caaatcggta ttaaaagaca tttctaatat
     8341 ctttgtgcca atgattcctg catttgtcgg tactgggatc gtggcaggga ttgccgcagt
     8401 tttagcaaac ttagtcacag ccggaacact tgatgcagca acatggcaac aatatatcga
     8461 tgtgatgaac atcttgaaaa acgggatgtt tagctaccta gttatctata caggtatcaa
     8521 tgctgcacaa gtttttggtg caacaccaac tcttggtgga gttatcgggg ccgttgtgat
     8581 gctgacaggg atgaatccgg aagcaccact gaaaaactta tttactggtg aagctcttgc
     8641 agctggacaa ggtgggatcc taggtgtcat cttcgctgtt tggctattgt caatcgttga
     8701 gaaaaaactg cataaagtag tacctgatgc ggtagatatc atcgttacac caactctttc
     8761 attgattgcc atcggtctga ttgaaatttt cttcatcatg cctttagcag gtttcatttc
     8821 tgatggcatg gtcggtggga tcaactgggt acttggcgtt ggtggagcat ttgccggctt
     8881 cgttctaggc acattattct tgccgatggt tatgttcgga ctgcatcaaa ttttgactcc
     8941 aatccatgtg caaatgattg atgaaactgg tcgtacgcta ttgcttccaa ttcttgcgat
     9001 ggctggtgca ggacaagtgg gtgcagcatt ggcattatgg attcgctgca aaaaagataa
     9061 agaattaaca gaaatgatca aaggagcttt accagtcggg atcctaggaa tcggcgaacc
     9121 attgatttac ggagtaacat tacctctagg acgcccattt attactgctt gtatcggtgg
     9181 aggtatcggc ggggcagtga tcggaatgat cggaaatgtc ggggctatcg ctattggacc
     9241 ttcaggagcc gctttgatcc cattgatctc tgacggtaaa tggttaggct acatccttgg
     9301 attattggca gcttatgctg gtggcttcgt tgctacattc ttcttcggta ttcctaaaga
     9361 ccgtttagaa aaagaagaat tagcgactga aacagccaca gtaaatgaag tgcctgaaaa
     9421 agtagtacct caaacttcag ctgaaagtgt gactatcctc tcatcagttg ctgacggaac
     9481 agttgtttct ttagataaag caagtgatcc agtttttgca caaaagatga tgggtgaagg
     9541 ctactttgta gaaccagaaa acggacatat ctattctcca gttactgggg tagtcagctc
     9601 tgtcttccca acgaaacacg ctattgggat cacaacgcca aatggtttag aaatcttgct
     9661 tcatatggga atcaacacag ttgacttagg cggtacgcca tttgatgtga aagtaacaga
     9721 agggcagcaa gtgacacctg atacgcttgt agcagaagct gacttagaag cgatcaaagc
     9781 agcaggcaaa gaaacatcca tgatcgtctt ggtaaccaat atggatcgtg taaaaaactt
     9841 tgtcttggaa aaaacaggta aagcaaaagc aaaagcacct gtgatggatg tcaaagctta
     9901 atatgataga aacaatagaa acgaaagaag aaactgaaat ctatttttca gcttcttctt
     9961 ttttattatt tattgttttc tgctaaaatc tgataaattc gtgtcacatc agatggtgtt
    10021 ccccgtaatt cataatctgc caagaaagga aagccaaatg tttgtgcata ttgtttggct
    10081 gttagacaat actgattatt gaagttttta tttccgctac cgactacacc aagacaaagt
    10141 ttatgattgt cttcatgttc cagatattca cgcatcgttt cggtcaagat ttctgtatca
    10201 ccgttatcta aaccatttcc gccgtctaaa taagtaggaa cgaaagtaaa aaatggttct
    10261 gtttctttcg taaaatcact attttcatgg atttctttta aagtaacttc aggattgaca
    10321 ggatcttctg catgcatttt ttcagcatat tctgctagat gtttggcaaa ggcacgcgta
    10381 tttcctgaaa tcgatatata taaaatgttc atggatcatc ctccaattct ttccttttag
    10441 tatagcatct tttttaagtt ttttggtggt gtaatttgga aagggactag gcgctttgct
    10501 tgtataatga gaaaaaagcc ggtagaatta tatttctatt aaagttttta ttaattaagt
    10561 aaaaataaaa aaggtttgtt atactttttg ataaaagcgg ttttattttg gaggatgctt
    10621 acatggatac agagcaacag aaaaaaggaa ttggtttaat tccacttgct gccttagtaa
    10681 ttggttcagc gattggaggt ggcgtatttg gtattatgac ggacatctcc ggatcggctg
    10741 gcggcccagc tattttcagt tgggtactcg taggaacagc aatgttgatg ctctccttgt
    10801 cgatcaataa tatcaataaa aaacgtccag atttaaaagg cgggatcttt agttacgcag
    10861 aagaaggttt tggccgtttc gctggtttta tcagcggttg gggttactgg ctcacatgct
    10921 ggttaggcaa cgtagctttt gcaacattgt tgatgagcgc attaggatat ttcttcccag
    10981 tatttgaagg tgggcaaaat ctggcatcga tcgtgatgag tacagtcttt ttatggggat
    11041 atgcatggct agtgaatcgg ggagtcgaaa atgctagttt cgtcaatgct atcgtcacat
    11101 tctgtaaatt agtgccatta tttttattta ttgtagtagc tatcagcacg ttttcgatcc
    11161 aacaattcat tgataacttt tctagcgaca ttatattgaa tgcgaatgga cagccgatcc
    11221 cgttttccag tcaagtattg agttgcatca tggtgatgct ttgggtattt gttggtattg
    11281 aaggagcctc tgtcgtaggt tcacgagcaa aaaaacaatc agatgttggg aaagcaacga
    11341 ttctagggat attcggcttg attattattt acgtcttggt ttccatgctt ccatatggga
    11401 caatgacgat gaatcagctg caaacgatca aggcgcagcc tgctatgggc tatgtgtttg
    11461 aaatgatggt tggtaaatgg ggagcagtcg tgatcaacgg cggtttgatc atttctttag
    11521 ttggtgtttg gctctcatgg acgatcttac cgattgaaac aatgggaaac atggctgaag
    11581 acggactttt gcctaaagca tggggaaaac tcaacaaaaa tggggcacca acttatgcta
    11641 tcgtattgac gacgttatgt acgaatgtct ttttactttc attattattc actgacagtg
    11701 cttataactt tgcttacaca cttggtacag cagctatttt cttcacgtgg cttttcttag
    11761 gattgtacca aatgaaactt tcttatcagc ggaaagaatg ggtccaattt ttagtagggc
    11821 ttttagcaag tgcctttcaa gtatgggcta tgctagtcgt agcctttaat gaagtaatgc
    11881 ttgttcttgt tcttttcttg ccaggcattt atttttacct caaagcacgt aaggagcaaa
    11941 acgagcctgt acaacatatc cacttagata agattttact tataggtgtg acgatggtcg
    12001 gagtgattgc aatggtttta tttgccactg ggatgcttca cgtatagaag tataaaagaa
    12061 gtataaagag aggttgtgag cctgtcgttt attcaaaaac atactaggaa aggagctgtg
    12121 acacaaatgt tgtcacagct cctttttcag aataaacggt gtttaagaga cagccaatcg
    12181 gcaataaggc tgaacgtctc tttcacagcc tcttttttga tgagtcatca tacatttttt
    12241 cgcttaaata cgagatatcc taatgctaag aacaaaacga tgtaagctaa attaccgaat
    12301 agcatttgat tagtagaaag atgagtcaat tgcttcatga tctcttcgtt tccaatttgg
    12361 ttttgcagat tcaacatact gatcggattc catttgatcc actcccattt ttggatgagt
    12421 gcaataagga taccagatat gattgacgaa gcaaaataaa agacgattcc tgctgagatg
    12481 gatgcgccgg aactattgat aaagcaagca atcatcaaca ctaaacttaa aatcagccat
    12541 aaaccgatat aactacccaa agtataagtg aacaagcttt ggatcagtgt ttgaccagta
    12601 gaaaccttct ccgtgaagga aatcgaaggg aaaagaatca gtttcatcaa cacagtgaca
    12661 ataatagcta aaaggtataa ataaacagaa taaataacta atgtgatcca tttgcttact
    12721 aatatttctc ctcgagaata ctttcgataa aggagatttt tcaatgtacc atattgtgct
    12781 tccatcgaaa tgatcgtgct tgcagctgca atcatgataa agacaatcca agatgtggca
    12841 ctaaaaagct gagcagtcat ttcaactgga tcaatgatcg tcgtgtattt tttggctaga
    12901 agtgctgttc ctatgagcaa aacaactaat agaacagata aaacagcagt tgactttttc
    12961 ttgatcaatt taaataattc ttgttttaca agcaatgtca tttttcttct tcccccttta
    13021 ctgaatccag aatttccaat agagaagatt ccaaatcatt cgtcagatgc tggacgtctg
    13081 tcacatggat attttgtgca gacaatagct ggatcacggt tcctaataat tcttcgtttt
    13141 cttttaaaag cttgatttta tcaggatctt ccaaagtata tccagcttct gtcaagatcg
    13201 ttcgagcagc tagatcattt gttgtagaga taacgatgaa atcttgcgct gtttggttca
    13261 attcagccat tgtacattgt ttgatgatct ttccttgatt gatcaggatc agctcatcga
    13321 ccattttttc aagctcgctg agtaagtgac tggagatcaa gaaagaggtg ccctgagtag
    13381 ccaagtcggt aatgattttt cgcacatctc ttgttgcttg tggatccaaa ccgttcatcg
    13441 gctcatctaa aataacgata gacggatggt tcagtaaggc aagagcaatt cccaattttt
    13501 gtttcattcc caacgagtag gatttcgctt ttttgtgaat gtacttttcc atcttgagct
    13561 gattcacgat agtgtcgata gcagatacat cgttggtttc agagaataac ttcagatgat
    13621 catagcctgt taagaatgga tagatacccg ggtattcgat caatgctcct acttgttcca
    13681 accctttatg actagaggga gaaatctcat tttcaccaat acagattgtt ccatctgagt
    13741 aatggatcag tcctaaaata gctttcatga tcgttgtttt tccagcgcca ttaggaccta
    13801 ctaatccaac gattttgcca ggggataagc taaaactgac atcgctgagg gcttgtttgt
    13861 gaccaaagtt cttggataat tcttggaccg aaagactttt caaaaatatt cactcctttt
    13921 ctatagatag tacaagcata cacctgatga gtggagaagt cgctaggtca ttggacttat
    13981 ttcttatttt ttcataaaaa aattgaatat taaataaaaa ggcttatttt ttctgatcag
    14041 tacttattcc aagtgtctag tggccgctag taatcttgat gccaatgata ctgatgagta
    14101 gcatccccac aaagaaccaa gtggaaagat tcagcttatc atgaaacaag accacaccaa
    14161 ttatgaccga accaactgcg ccaatccctg tccagatagg ataagagaga ctcatcggca
    14221 aacttttgat tgcctttgac aaaaagtaaa aactagcaat catgccaatc actgtgtaaa
    14281 aagaataatt gattttagaa aaaccatcac tccatttcat catcgttgac cagaatactt
    14341 ccaaaattcc tgctatgatt aattccatcc atgccatact tacattcctc cttaactata
    14401 aaaaaataca caaaaagagg aaaatcatcc cttcaaagac ctaatggaat gatcatcctc
    14461 tttatcctac ccggtggcag attgtatatc tttttctgta gtatagcaca cctatccaga
    14521 tatcggcaat ctcatacttt ttagtaaagt gtaccaagca ctgacggata tatcgttagc
    14581 ggaaaagaag atgaatgctt tgcaatttca tagaaagaag ttgttattta ttaatcattt
    14641 aaaaaaaaca gtaactagac cttatttcta tatattatcg g
//
LOCUS       DAZBSA010000068        14631 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000068 DAZBSA010000000
VERSION     DAZBSA010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.068"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..632)
                     /locus_tag="ACHGMT_001945"
     CDS             complement(<1..632)
                     /locus_tag="ACHGMT_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFN6835680.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAY"
     gene            complement(880..1716)
                     /locus_tag="ACHGMT_001946"
     CDS             complement(880..1716)
                     /locus_tag="ACHGMT_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFN6835681.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(1727..2656)
                     /locus_tag="ACHGMT_001947"
     CDS             complement(1727..2656)
                     /locus_tag="ACHGMT_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFN6835682.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2848..3201
                     /locus_tag="ACHGMT_001948"
     CDS             2848..3201
                     /locus_tag="ACHGMT_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFN6835683.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(3275..3931)
                     /locus_tag="ACHGMT_001949"
     CDS             complement(3275..3931)
                     /locus_tag="ACHGMT_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFN6835684.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            4157..6091
                     /locus_tag="ACHGMT_001950"
     CDS             4157..6091
                     /locus_tag="ACHGMT_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFN6835685.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(6177..7400)
                     /locus_tag="ACHGMT_001951"
     CDS             complement(6177..7400)
                     /locus_tag="ACHGMT_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFN6835686.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(7394..9265)
                     /locus_tag="ACHGMT_001952"
     CDS             complement(7394..9265)
                     /locus_tag="ACHGMT_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFN6835687.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(9286..10404)
                     /locus_tag="ACHGMT_001953"
     CDS             complement(9286..10404)
                     /locus_tag="ACHGMT_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFN6835688.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(10487..11320)
                     /locus_tag="ACHGMT_001954"
     CDS             complement(10487..11320)
                     /locus_tag="ACHGMT_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6835689.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(11313..12230)
                     /locus_tag="ACHGMT_001955"
     CDS             complement(11313..12230)
                     /locus_tag="ACHGMT_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6835690.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(12457..13581)
                     /locus_tag="ACHGMT_001956"
     CDS             complement(12457..13581)
                     /locus_tag="ACHGMT_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFN6835691.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            13764..>14631
                     /locus_tag="ACHGMT_001957"
     CDS             13764..>14631
                     /locus_tag="ACHGMT_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN6835692.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKY"
BASE COUNT         4624 a         2887 c         2351 g         4769 t
ORIGIN      
        1 atataagctt ttaaatcttt acataaatac tcatattcga gtgatttttt atatttttta
       61 attgtgccgt ttacgagaat ctcttcatct ttcaatacta ggcctatttt atttttataa
      121 cctggcatgc tcattagata aaggatttca tcgtaatgga tatagtgatc gctattacca
      181 taacggacag ttgtccacct ttttttcgta gcatgttgaa caggatagtt ttctttttct
      241 ttcaataagt aactttggat gcttttttta tttttttcat aagtgaattc ttttatcaaa
      301 taaccactag gcgtaatctt tcgattaata acagttattg ctttattcgg atgagacgtt
      361 agataaacaa ttttacactt ttcattcttt tctctaagca tttcgcctag ttcgataccg
      421 ctataatagg cgttcaaatc gatgtctagg aaaaaaatat ctgaggaata gatttcatta
      481 tttttaatga attcaacaaa ttgtataacg ttcaatccct gaacgatctc aaatttccaa
      541 ggacttaata aaattctgga ttcttccaaa atttctacta gcattttttc aaaaaccaaa
      601 ctatcatcca aaataaatat acgcgttacc ataaaaagta cctccactcg atattttact
      661 atcaaaatca tttcatacaa cttttcaaca tacatgttag gaataaattg taattctctt
      721 cacaatcttt tccaacacct ttgaaaccga tatcaaatac tttcaggatg ctgcttctaa
      781 aataaatatc aaacaaatga atacatcatg caaaaaaagc cagacaaccg acgtccttgc
      841 gtgtcagctg tctgagctat gtttatttat tttgttcttt taaaaagaac gatacacata
      901 ctgatttttc ggagcagaga ctttctcagc gtttgtaaca actacagatg ggatttcacg
      961 cttgaatggt tgataatagt ctgattggat ggttccttct attttatacc attcatcatt
     1021 tttaaccgtt actccttccg gcatatgcgt caacaaacca aagactcctg aatctgccac
     1081 acaatggata atgccgaatc ggaacaagaa gatatctgta ttgctatcgt tcggtgtttg
     1141 gtaaacaaat ccttcatagc tgattttttt gccaatgaat tcgctagggt aattataaat
     1201 tagctccata acttctaaat agttttcatc tgtgatcttg atagtttgct tatccccata
     1261 cttttctttc aactcattca tttggtctaa gtagtctgat ttgttgaaat aaatactggt
     1321 atccggcttt aagtattgtg tctgcatctc cggatcccct actgattctt tacttactgg
     1381 gaaattaaat cctttggctt ctacgatcgt agtgtcgaga ctaactgttg gaaacaacgt
     1441 ccccacgatt actggcaaag ctagcagtac ataagcgatt ccacgttgat aaggttttga
     1501 caacccatgg tcatgatcgt gctcgtgtgc cttttcttct gcttttccgt ttttgaccca
     1561 caaatacagc tgaactaaag ccaaaagagc agataaaatc attgaaagaa tagctaagta
     1621 gcgataatgg acattgatat attgatcgag tttgccagaa acttgcaagt acatcatcaa
     1681 ttcgaaatac ccagataaaa tcaaaaaccg cagcatattt tccctcctaa ataaataatg
     1741 tgaatccaat aacgctaagg gcaatcagtc cgatcagcga taaaataaag cgtcctgtaa
     1801 aatatcgttt catcatcagt agatttttga tatcgaccat tgggccaaag accaaaaagg
     1861 cagctacagg tgctgtgccg aacagactta acaaagaaga tcctataaac gcatctgctt
     1921 ctgagcaaag agacatcgta aaggccaata acagcatgat gataatggct aaaatctttg
     1981 ttgagccgag cgttagcata attctagttg gtaaataagt ctgcatcgca gctgcgatta
     2041 atccgccaat gatcaaataa cgacctgtat caaaaaattc gtcgattgaa tgtgtcaaca
     2101 catgcccagt ttgatggatc agctgatttt ttggttcatt cttctcactt tgttcttctt
     2161 catggacatg atgatggtga ttttctgcta ctttagacgt gattttttct ttcaatacag
     2221 cgtctttttg aaaataggca agccagacac caatcacaag ggcaacaaaa aagctgccta
     2281 tcacccgcca gcttgccatc tgccaagaat tgccaaatgc gataaaagtg gaaaaaatca
     2341 cgattggatt gatgatcgga gcagtcagca taaaagcaaa cgctgtgtga actggtacgc
     2401 cttttttaac aaattgatga acgatcggta cgatgccgca ttcacatgaa ggaaagaaaa
     2461 atcctaagat cgaaccagta aaaatagaga agatacgatt ttcaggaagc catctcttca
     2521 cacgttctgg ggtcaaaaat acttgtaagg cgcctgagat aagacagcct aataatacga
     2581 aaggtaatgc ttcaataacg atcgataaga aaatcgtact catctgcaaa accgaatttg
     2641 gaataaaaga aaacatgagt ttcctccttt ttatttctag ttcattcgct ttgcaaatat
     2701 acgcttttat tcaaaaaaaa caagttgctc tgtaaatttt tacacaactc ttatttttta
     2761 ccaaagcact tcaattgact ttttctgtta ttcccattaa attaaggtct catacatatc
     2821 acttctatca taaaagggag aataaaaatg aaacaaacca ctaacttgac tgaagttcaa
     2881 gacatactgc aacagtcatc cgttgccgtc atttatcttt cgatgcctaa ttgcagtgtt
     2941 tgtcacgctg ttcgtccacg actagaggaa cttctcacac attatgatat tccggcactt
     3001 catttggatg catttgaaac accagaagta gctagtaaat ttgaagtgct gactgcgcca
     3061 gttgtcctga tttttcatca aggaaaagaa gtctttcggc aagcacggtt catcgacttt
     3121 aaaaaaatcc gttatctact agatgaactg acagcagaag atgattccct ttcttatgaa
     3181 gaaatcttcc gaacaaaata atcgttgtcc aaacagaaaa aagcgattga acaagtgaaa
     3241 gttttgcctt catttgttca atcgcttttt atttttattc tgctgtattt tcggaatgcg
     3301 catttgcgtc agaatgcaag aaatagatca aggtttgcaa ttcattcgtc aaatcaacat
     3361 tttggatcaa tacatctgac ggagcattca atcgagcagc ggtaaagttc aagattcctt
     3421 tgattcctgc ttccgttaac tgattgacta cttcttgtgc tctttcagcg ggtatcgtta
     3481 aaattgctac ttcgatttgt tggatcttga tttgttctga catatcttcc attggataaa
     3541 cggggatacc gtctacgatg cgcccaacga tttcctcatt cacatcaaaa gcacaactga
     3601 tacgtatgct attactttga tggaatttgt attttagtaa agcacttccc aagttcccta
     3661 caccgatcag cgcaacattt gtcaattgat cgtcgtttaa tgtctttgca aaaaagtgca
     3721 taagatcttc tacatcataa ccatacccac gttttcctaa ttcgccaaaa taagaaaaat
     3781 ctcgacggat cgttgcacta tctacttgaa cggcttcact taattcagtt gaagacactt
     3841 ttttctttcc agcattattt aaaatcctta aatagcgata atacaaaggc agtcttttag
     3901 ctgtcgcctt tggaataata tgatctttca cgaaatgacc tccacgtatt gtctaattac
     3961 atctttgatt tctccatcat agcagtattt ataggaaaaa atcaattcca ttgctcagat
     4021 attgtgaaat tttattcttt attcatttat ttatcatttc tgcaaatctc gcagtataat
     4081 ggaatttatg gtagactact aattgaaaaa tagtttttta tttgaggaga attcacaaag
     4141 tagaaaagag gattccatga tattattaca agccaatcag attgctcgcc attttggtgc
     4201 agaagtctta tttgaaaaca tacatttaga aatcagtaca ggcgctcgtg tcgctttagt
     4261 tggcagaaat ggcgctggaa aatcgacttt attaaagatc attgctggaa ttgaagcacc
     4321 tgatgaagga aagatcgcta aaaataaaac agcgactctc ggatacctcg ctcaggacac
     4381 aggacttgat tccaatgaaa cagtttggaa cgagatgctg aaagcttttg aacaagttag
     4441 acaaatggaa gagcggatgc gcgagctaga gattttcatt agcgaaggta cgccagacac
     4501 acctgcttat agttcttatt taaaagaata cgaccggctg caacacgatt tttcagatcg
     4561 aaacggctat ggttatgaaa atgaaattcg ttccgtattg catgggttta aattcgattc
     4621 ttcattttat gatcagaaaa tcgatacttt atcaggcgga cagcgcacac gtttagcttt
     4681 agctcgcatg cttttgcaac accctgacat tttgattcta gatgaaccaa cgaaccattt
     4741 agatatcgag acactttcct ggcttgagaa ttatctgcaa agctatactg gtgcattatt
     4801 gattgtttcc catgaccgtt attttttaga taaagtagtc aatgaagtct atgaattaag
     4861 cagaagaaaa atgcgccatt acaaaggtaa ctattcgaaa tatctagatt tgaaagcaga
     4921 acagttagca agtgaatgga aggcttatga aaagcagcaa acagaaatcc ataaattaga
     4981 agacttcgtt gctcggaacc ttgttcgcgc ctctaccaca aaacgagcgc aaagccgcag
     5041 aaagacatta gaaaaaatgg accggctgga tcgtcctcaa ggggatgaaa aacaagcaaa
     5101 ctttctattc agtatagaaa agccttctgg aaatgtagtc ttgcaaatcg aagatgccgc
     5161 tatcggatat gataatgtca ccttatctga accaatcaat ctagatatcc gtcgtcaaga
     5221 agcgattgct ctagtaggtc cgaatggtat cgggaaatcg acgttgctga aatcaatcat
     5281 tcaccagatc ccttttatcc gtggaaaaga aacgctaggg acaaatgtca ccatcggcta
     5341 ttacgatcaa ggtcaagctg atctgcatac aaacaagacg atcctagcag aattgtggga
     5401 tgagcatcct accactccag aaaaagagat ccgaagtgct ttaggtgctt tcttattttc
     5461 tggcgaagat gtagaaaaac caatccctct gctaagtggc ggtgaaaaag cacgggttgc
     5521 tttagccaag ttagcgatga acaaagaaaa ttttttgatt ttggatgaac caacaaacca
     5581 tttagatatt gacaacaaag aagtattaga aaatgcattg atcgattatg acggcacact
     5641 gctttttgtt tcccatgacc gatatttcat caatcggatc gcgacaaaag tcgtcgaact
     5701 ctctgaaaat ggcagcaaag tctatctagg agactatgat tattatttag agaaaaagca
     5761 ggaagaagaa gaaatcgccg aattgttggc acaagatgag ccagaatctg ctcctgctcc
     5821 cacgaacaaa tctgattatt accaaagcaa agaacagcaa aagcttatcc gtagccttca
     5881 acgaaaaatt actcaggttg aagaagaaat ggcgcggatc gatgaatgga tcgatcagtt
     5941 ggaacaagaa atggtcgcgc cagaaaacct ggaagatcac gtaaaactca atgagctgaa
     6001 ccaagattta gaagctgcta gacaagatca agaaacaaaa ttgacggaat gggaagaact
     6061 aagcttggaa ttagaagaat atgaaaatta acgaaaaaac caaaacagaa accattcatg
     6121 ctgttttggt ttttcttttt ttattttatc aaacaaaaat tccatattta cctttttcac
     6181 caataagaag accaattact agtcattctc actaaagctt ctaaataata gtaatcaccc
     6241 caaatacaag gtgtatttac cccattattt ttgggcttat tataaacaga ctgtgttaaa
     6301 aagccatctt cttcttccgt tgtagcatat ctttctttca attgtcgcat aattttccaa
     6361 acagcagact ggtataagtt cttttctgtt ttgtctaata gacctgattc tattaattct
     6421 aataatccac aaaccatgat caccatacta gatgtatctt tttcttctct gctagaatca
     6481 tcaaagatca aatcccaata acaaatggta tcttctggca accgattcat aaaataattg
     6541 gctaatattt tgcttaaatc aaaatatttt tgttctttca aataacgata acttaatgca
     6601 aaaccgtaaa tcccccatgc ttggcctcta gcccaacagg aattatcact ataaccttga
     6661 tgcgtacttc catatcttgg ttttccatct ttgacatcca tataaaatgt atgataagtt
     6721 gaaccatctt ctcttactaa ataatctgct gcattttgaa tatgagcctg agcagctttt
     6781 ttatattttt gatttccagt ttcttcactc gcccaataca ataatggaag attcatgcaa
     6841 caatccatga tcattctacc ttgttgtgaa ggatcactag gatctcccca agcttgtata
     6901 actcctgcct tctcccaaaa acgctgaagt aatgtttctg cagcatcaat tgaagcttcc
     6961 ttcatttttc gatttcccgt tattttataa tctgctacgg ttgataaaga atacaaaaaa
     7021 ccaagatcgt gatgattagt gataatttga cagtcaatcc tgtggataaa atctatatct
     7081 gctttttcag ctgtttgttg aaataaagga tcacccgtct tctcaaataa caaccaatta
     7141 attcctgtcc aaaatccttc tgtccaacca atattgtctc ttaatggata acgtcctttt
     7201 ataggacact cgcttgggaa ttgatactga ctgttactga tacgtttgat taacctctcc
     7261 gtgtgtctgc gacaacgctc gatctcatta ttccattctt tttccattaa ttctttataa
     7321 cattcattta tccatttttc tggatgtatt atctcttcaa acatcacttt atcaagcatt
     7381 tccccaccag cctttagcat aattttagtc tttttttatt gtttttattt tggatagtta
     7441 gcccggttgt cccatcaaaa tcaattttgg gacgattgtt ccatacttct aaactataat
     7501 cccgatcttg atgagcaacc actcctgttc cgagccaatg ggttcctttt ccaaatgtac
     7561 cctctaatcc aggaataaaa gtgttttccc atgtcatcaa gtttgtattg ggcaaaccca
     7621 taatagctgt ggattttcgt ttaatagtat cgtcacttaa acagataata cctgaaaagc
     7681 cagcttcact caccctatag ttttccttgt cttcttctat gattattcct tctttctcag
     7741 gagaacattc aatagcaaac cctgtctcat atagaacata ttctgtatct aattctactt
     7801 tgtgtacgcg aatctgccag ccttcaaaag gaataagcca agttctgatt tttgcctgta
     7861 aagtaggttc ccatgtagag gcaagtacct ctttcttaca tacaatgtct cgtacattat
     7921 ttcgaacgat gaactgtcca taggttttga tgctctcatt cttaaaactg gtcgtgatat
     7981 atgtgtccgc tcgctgaatc cctagcattg aatccattgc catttctgct ttcaattgat
     8041 tcgctcgcgg tatcgaaaaa ccaaattgac tagaataagc aaattttaaa tatttttcat
     8101 tggtcaatgt atggaaattt ggccctaatt gtcccgcatt caaaaaaaat gtatgtccat
     8161 tatcatgttg tatgagcaaa ttggcttctg aaagaagttt agttccttca cgaataggca
     8221 taggcatttc tttagccgtc caatactcat ggtcttcagg tagcgccaac aataaaaaaa
     8281 tcttattcag ccataatgga gataacgtac tattataggg ttcagtcaat gtcaattgag
     8341 ggtaggtata accaatattc aggatacctt tttcatcaaa tatttctttt tgcatccacc
     8401 atcttatatt ccgattgact aaccccttga tttcacctaa tttaaaaggc aaaagatcgg
     8461 cccacaacat agctgaccaa aagcttgaaa ctgcaaaacg ataaatcatg cttcgaccaa
     8521 aaggcacatt tgcaccatct tccgcaaaaa aatgaatata gtctctagaa aaatcaatag
     8581 ctcggttctt atatatctgg ctaatgggct tttctggttc tagtacgcta taaatcaaac
     8641 cataataatg aaatgcaaaa ggattataat aatcttggcg acctcttgaa ctgtctcgat
     8701 accagccatt ttgaagataa caattttcta tattatcaaa tgcttctttc agtgctgatt
     8761 ctttaactgg ataaccaaat ttttggataa ttgttccgac aattatctta aaaaattgcc
     8821 aattaccatc tgcacattca tattcaaaaa tttgcgagaa ccaatgaata atctggcttt
     8881 tttctcctgt atctaatttt ttccataata attgttcatg atgatataac gctaataaca
     8941 ttgcgggcat ttctaccata cgctggtctc tttcatgtat ttctccccaa taatcgggat
     9001 gggaaggatc tgttccagct tttattcctg atagaattaa tttcaaccat cgttcatcta
     9061 attggtcaat tgcgggcccc gctccccata aaacccgaga gaatccttcc attcctgcaa
     9121 ttttatttcc atatcctacc ccttgatgta tataatcaat ttgtgcgcca cttgctcgat
     9181 aaaacggctg gagtggttga tttatctttt taaataagtc aaagtaatcc tgtctggtcc
     9241 ttaatagtgg tactggatgt ttcattgtat tttctcctct ttttactacc aaatagctaa
     9301 ttccgactgt tctaatcgaa gtaagccttc aataaaaaaa taatcagaat agatcagtgg
     9361 cacattataa gtcagttctc tatggtatgc ttctgctccg cctccaatga tcccatcttc
     9421 atccatattc caatttgcat agtgcttttc tgtcttttgg agtatattga tagcagcttc
     9481 atgatacatt tttgatcgta cagaggaaac atatcctgct aattccagaa gaccgcatgc
     9541 agcgcatact ccagcactgg tatcaatatt cgaatctttt acagcaaaat caatgagagg
     9601 aatataacca gttttttcga ttttctgaat aaaatttact gctatctttt ctgctatagc
     9661 tagatagtac ggattttttg tgtaacgata acttaacgcg aatccatata aagcccatgc
     9721 atttcctctg ctccaacaag aattctcatt gtatccttgc ccgccaagta accgaatata
     9781 tttccctgat tctgcatcaa attctgcgat atgcccggtt gatccatctt tcctcactag
     9841 atacttagct gttgtatctg catgctgccg agcaatttta gagaacctcg gatcaccagt
     9901 ttccctcgaa gcccaataca gtaatggtaa attcatcata ctatcaataa tcatccaacc
     9961 aggtttatgg tcattccatg ctactaaata gttccctgaa atattatagc gtccagcaag
    10021 taccgtagct gcatgtaacc ctagttgtcg actatgtttg ttttgagact gttgataatg
    10081 tgcaatagcc gatggtaaga ataaaaaacc tacatcatga tgaacgtctg aaaaatggta
    10141 cagggcagat tctaataatt gttcttgttg tattgctgcc tctttgaact tttctcgttt
    10201 tgtcttgtga aagagctgcc ataaaatacc actccaaaaa ccattggtcc accagtcgat
    10261 tcctctgtcc tttattacgt ctgtatattt tccgttgtaa gaaatatatg gaattttatt
    10321 ccccattctg tctaattccg catctatctt ttcagaagct acattcaaaa tttttcttga
    10381 ccattccttc gtctgttcat tcatggatat cccgccattc ttttaatttt tatgaatgtg
    10441 gagttgctcc tagaacgaac tagatggtta acattcgtta ttttttttac ttatttgcag
    10501 ctccaatgtc attctcctta aatttagact gcgcccacac ataaatcaag accattggga
    10561 tcactgaagt tgctaaaatt agcataattg cgccataatt aattggctca cctgtactag
    10621 aaaccgtcat atttttgata attaagggaa ccgtatattt atctttatct gaaagcatga
    10681 ctaatggaaa tagatattga ttccaacttc ccataaagat aagtaaacca ctagtaataa
    10741 tcgtaggtga taaaatcggt acagcgattt tcgtaaaaat cacccactca ttggcaccat
    10801 cgattctggc agcctccatc agtgctgtag gaaattgact gctatattgt cgcatcataa
    10861 acactgaagt tgtactggca atcgaaggga tgatcacacc tgcataagta ttcgataatc
    10921 ctaaacgact cataatgtaa aataaaggaa tcaatgtggt aaaaaaaggc accatcctag
    10981 atagcataac tgtattgaac aacagtttat tccccataaa acgatacttt gctaatgcat
    11041 aacccgccat tgtagaactg gctgtagcta aaactgtact gattacagca ataacaatcg
    11101 aattccaaat aatcaaggga taattatatc tcgccatgat ggcttgccag ttttttaata
    11161 gcatatcccc aaacttgaaa ttgattccgc cacttaaaat ttcttgatta ctttgcgttg
    11221 aagctacaat catatataaa tatggcatca aaaatattaa ggcaccaaac acaataatga
    11281 cgtacgtaaa aattctgctt tgtatctttt gtttagtcat ttttatcacc tccgaattta
    11341 aattgaatag ccgataatat aacagataca aacaccatcg tccaggcaat agcagaggct
    11401 tttccaaaat caaccatttc aaaacttgtt ttatataaat tgactcctac gaccaaagct
    11461 gaattttctg gtccattgga agtcccaaag aatatatttg ggatattgaa attacccaag
    11521 ccattgatag tggctaaaac tgtggccatt aaaatgattg gctttagcat ggggatcgtt
    11581 aataaaaaga attgccgaaa tttcgatgct ccatctattt cagcactttc gtatatctct
    11641 ttagaaatcc cttgaagacc agctaaaaat aaaatagtgt tatatcctac actaagccaa
    11701 atcatcgtga tgatcattga tattcgtatc caaaaaggat ttgttaacca ctgtacaccg
    11761 ttaattccta gcgcattcaa aaatccattg aataacccag tcggattgaa aaaagtcatg
    11821 aatacggaag ctacagccac cgttgatgta atggttggaa tataatagac agttctaaaa
    11881 aatccttttc cttttaactg tgctgaattc aataaaacag caaagtaaat agctaatagc
    11941 atcgttattg gaattgatcc tgccatgata atcaaaacat tcgtgtaaga tttaataaac
    12001 aatggatctc ggaagatagc tagataattg tcaaatccaa caaattcaaa tgagtagcca
    12061 attttcataa aactaaaagc aaacgagata atgatgggaa tcaaaatcgt cacaataaag
    12121 aaaataacaa aaggtgtgac caatccataa gcactttttt tatgagacgt atctgttgct
    12181 tttttcttgc tgttctttat tgtcttgctt ggtaacgaag attgcatcat cgttaagtcc
    12241 tcctaatatt ttttaatcac cagttaattt tacgccgtac ttatgagcaa aattttctgc
    12301 ttcttttaga tatgaatttt caaaatcacc tgtttgccaa tagttatatg cagcggtagt
    12361 caaataagac gtagcatctg aataagtggc tggtaaaaca gtatttgatg tattttcatt
    12421 gattttattc aatagttcat agtatttttg tcctccttag tagtcaaact ttttctcagc
    12481 tgcttctgtt tgataggcca atgagttaga tgtagccacg cccagctcca gtgcgttggc
    12541 taagcattct tcatctgtca gcataaatgt aagaaattgt tgtgccactt gtggattttt
    12601 tgcatcttta gggacataaa aacttgatcc tcctgatact gcaccttttc ctggattttc
    12661 ttttgaaaaa ggaatcagtg gagccatgcg ccatttgtca gatgcatcag gaaaattcaa
    12721 agacatattc gtagcatacc agccgccagc aacaaacatt gcagattctt gaaatgcttt
    12781 ctcagcatct tgtgcaccgt agaaatgtac aatatctgag tcgatcattt ctttgattaa
    12841 ctttgcagca tgtttcgctt ctttagtatt cagacggata tttccttctt catcaagcaa
    12901 agattgattc tgctgggcta gcatgttgat aaacaattct tggtccccag attctggcaa
    12961 ggcaattcca aaactaccag ttttttcttt taatgctttt gccacctgga tatattcttc
    13021 ccaagaactg atttcttcaa aatctacacc tatttcagca aatttttttg gttgataata
    13081 acaaagtgct ggggtaaaat cactagccca aggaataatc gtttcattac tcgtttgttg
    13141 acgcaaaata ttcaattttt gatcataaaa ctgatcgcca tatttgtcat aaaatccata
    13201 ttcatcagca ctataaaaag catcttgaaa tttttccagc acacctgctg ctgaagtgtc
    13261 tgccatggag ataatatccg gcatatcttc gtgtgttacc aattttgggg tgatcatttg
    13321 tactgttgct gttcccgaag ctacattcac aatttttaag tcaatatttg ctccagtttt
    13381 gcggttatag atttttgcag cattttcaac atagtttgtc agtttgccgt ttgcgacata
    13441 tacttctaat gtagtggctt tttttatcat tggatctgcg atgtttgctg ttacttcttc
    13501 gttagttttt ccacatgcac ttaagctgaa taatagggta atgtttagca aagctatcac
    13561 caatttgtat ctttttttca tgtctatccc ttctttcttt tcaagttctt atataaaacg
    13621 ctttcactta tgtatattaa cggaattagt aacacaaata tttatataaa aaaatatttt
    13681 acttgtcgtt ttggcttttc atttttgtta taatagtcat taattataaa tagaaagctt
    13741 tttatataaa aagagaaagg aaagtggttc ttttgttaac aaacaaccaa tcctccaatc
    13801 ttgtcaaaaa aacactaacc tattccatag atacccatct tcatttatct gctgctccct
    13861 attttcgact actaataaat gaaagcgcta atttatatgc tgaatttgca cataaatcta
    13921 tagaactaga taatccttcc cttttattca ttgataaaca gcaagaagtc caaatctttt
    13981 ctactcaaaa ggaaacacaa acttttatca ttatccagtt ttattttgat gagcaattat
    14041 tagcacatat tacgggacaa tctttttcta tagatcaaga aataacatct atcattactt
    14101 ttctgaaaaa tgaacagttc aaaattgatt atctaaaaca tagtaagcca gctatttcct
    14161 atgatcagga aatcaattct tttttcatac tatgccaaca gatgagcatt catttgcttt
    14221 ccttattagt tacttattgt ctgcaaaaaa ttcaattgtc ttccttacca aggcattatc
    14281 aacaggaact tgctttattg acaatacctt caaatgagaa agtcattcct aaaaaaaagc
    14341 atattcttta ttcagatgaa ttagttgatc caatcatacg atatttagaa gaacatcttt
    14401 cagaaaattt tttgcttgaa caattagccc aaattttttt tatcagtcca gccagcttaa
    14461 aaaaacattt tagaaaagtg aatggaaaaa gtgttattag ctattttaaa gaactaaaaa
    14521 tggaaaaagc aaaacgtctc gtgctagaaa atgaattaac ctatacagaa attgcagata
    14581 tattaggatt tcattcgatc catcattttt cttcagcttt taaaaaatat a
//
LOCUS       DAZBSA010000069        14438 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000069 DAZBSA010000000
VERSION     DAZBSA010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.069"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(228..1115)
                     /locus_tag="ACHGMT_001958"
     CDS             complement(228..1115)
                     /locus_tag="ACHGMT_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6835693.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1112..1981)
                     /locus_tag="ACHGMT_001959"
     CDS             complement(1112..1981)
                     /locus_tag="ACHGMT_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6835694.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2118..3404)
                     /locus_tag="ACHGMT_001960"
     CDS             complement(2118..3404)
                     /locus_tag="ACHGMT_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN6835695.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3677..4255
                     /locus_tag="ACHGMT_001961"
     CDS             3677..4255
                     /locus_tag="ACHGMT_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN6835696.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4255..5184
                     /locus_tag="ACHGMT_001962"
     CDS             4255..5184
                     /locus_tag="ACHGMT_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6835697.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5181..6917
                     /locus_tag="ACHGMT_001963"
     CDS             5181..6917
                     /locus_tag="ACHGMT_001963"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN6835698.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6980..7816)
                     /gene="pheA"
                     /locus_tag="ACHGMT_001964"
     CDS             complement(6980..7816)
                     /gene="pheA"
                     /locus_tag="ACHGMT_001964"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFN6835699.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7813..8322)
                     /locus_tag="ACHGMT_001965"
     CDS             complement(7813..8322)
                     /locus_tag="ACHGMT_001965"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFN6835700.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8315..9610)
                     /gene="aroA"
                     /locus_tag="ACHGMT_001966"
     CDS             complement(8315..9610)
                     /gene="aroA"
                     /locus_tag="ACHGMT_001966"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFN6835701.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(9623..10714)
                     /locus_tag="ACHGMT_001967"
     CDS             complement(9623..10714)
                     /locus_tag="ACHGMT_001967"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFN6835702.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10731..11897)
                     /gene="aroC"
                     /locus_tag="ACHGMT_001968"
     CDS             complement(10731..11897)
                     /gene="aroC"
                     /locus_tag="ACHGMT_001968"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFN6835703.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11899..12963)
                     /gene="aroB"
                     /locus_tag="ACHGMT_001969"
     CDS             complement(11899..12963)
                     /gene="aroB"
                     /locus_tag="ACHGMT_001969"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFN6835704.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12996..14018)
                     /gene="aroF"
                     /locus_tag="ACHGMT_001970"
     CDS             complement(12996..14018)
                     /gene="aroF"
                     /locus_tag="ACHGMT_001970"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFN6835705.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4112 a         3144 c         2609 g         4573 t
ORIGIN      
        1 tgacaatcag gacagtcatc ggcaatatag cagcaatcac ttctttaaaa ttttttagca
       61 tgtatcctcc tcctttactc tttaagttta ccttaaaatg aaagagtttt cgagcacaaa
      121 cttaaaacgt tatcatttaa tttttctcaa cataaaataa cacacgtccc aaaagtatct
      181 ttttacttgg aaaaatgact ttgaaggaac gcgcttattg tgttttttta ttttacagag
      241 ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa atacgatggc agttggtaaa
      301 gtagcgatga ctaatccagc catgatcatt ccatagtctg gtgtatagga agcacccata
      361 gcagataaaa caagcggaac cgtccgttta tcaggagatt gcaaagctac taagggccat
      421 aaataattgt tccagctact catgaatgtg atgattgcag ccgcggcata ggtattttta
      481 gccgtcggca tatacactct aaaaaaaata cttagttctt ttagaccgtc taatcgagcg
      541 gcttctacta gatcttttgg aaaagattta acattttgtc gaaagaagaa gatcaaaaaa
      601 gcagtgctaa cagatggcag gatgacaacc aagaaactgt tgattcccaa aaccgttccg
      661 ttcaattgtg aaaacattcg atataaaggg atcatcaatg ccgcaaaagg aaccatcatc
      721 gatagcagta gaatattgaa aattaggtct tttcgtttac tttgataaat ctcaaatcca
      781 tatcctgcca ttgacgaaat cagcaatgca aacacagtgg tcaccaaagc gatcatcgct
      841 gaattccaaa gggaacgagt aaaattcagc tcattggaaa acaaattttg aaaattgatg
      901 atcaagttac tgccaatctt tatttttccc gatgtgatat ccacgggtgt attcgtcatt
      961 cccaaaacca tccagacaaa agggaaaatg gaaataaatg ataggatgct taatgctgta
     1021 tactttaata ctgacccaat tgtcgaacga agttctaatg cttgtttttt gacgttgggg
     1081 cgttcgattg tttctttttc taccatattt ttcatcgact ttctcctcct actttcgttt
     1141 ggattatcga caatatcaca ataaaggaaa caataacaaa ggaaactgct gccgcatatc
     1201 caaaattagg cgtgaattta taactcaaat tatagatgta ttgagatagg gtggtcgttg
     1261 catttgctgg tcccccgcca gtaatatttt gtacttcatc aaaaagctgc aatgtcccga
     1321 tcgtcgaagt aatggaagta aacaaaatga ttggttttaa atttgggatc gtcactttga
     1381 taaactgctg ccatttagaa gcaccgtcaa tagatgctgc ttcataaatc tccgaatcaa
     1441 tattttgcat gcctgaaagg aaaaaaatca tattgtatcc agtccaacgc caagtgatcg
     1501 aaagaatgat cagaactctc gcccaaattg gatgagtcaa ccagccaatc ggtgtgtcaa
     1561 tcataccaat gcccaacaac aagttgttta caaggccaga ttgagaaaat agacttttca
     1621 tgatcagaga ataactgacc atcgaagtga tacatggaag aaagatagcg gttcgaaaca
     1681 accctttata tttcaacttt ttatcattta aaaggttgga aataaaaagt gctagaaaca
     1741 acatgatcgg tacttgtaca agtaaataca ggaatgtatt aaaaaaagct tttttaaaag
     1801 ttgcatctgt caacatccgc tgataattac ctaacccgtt aaatgtcata ttgacagggg
     1861 gaccactttg aaaagacgtg atcaaagcca aaaccatcgg aataaaaacc atgattgata
     1921 tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt gaataaatct ttctgcttca
     1981 ttcttttccc tccaattcta tcattgatta tctacatgaa aagacaagaa agacaatatc
     2041 tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct gatgaaaaag cgaagaaatt
     2101 cactttttta ccaccgatca attagccaat tgtgcttcta cttgtttttg tgtatctgct
     2161 agtaccttgt ctgtctcttc tccattgatg attcgatgaa gagcttcagc tacgactgct
     2221 tcaattgcat aggtctcatg tccgtaattc acttctggta tttcagatgt ccatgttgaa
     2281 aaatcgtcaa agatcttttg accactgtaa aattcagaag cttcttgata agcttcttgt
     2341 tcattcgcgc taagcatcgt agatactagt ccgatttctt tcgcaagagt gcccatcagt
     2401 tcttcattcg aagcaaacgt tgcttctagg aaatctttcg ctgaatcttc tccagctact
     2461 cctttgatca cataccagcc agcgccgcca aggttagaag cttgcgcttt ttgcagattt
     2521 tctggaagtg caggtattgg tgcaatcttc cattttccag attggttttc tgctccttgg
     2581 atcgttgaag aataccaagc gccagttggg ctagaagcta ctgctcctga ttgtatcgca
     2641 gttactccag cattccagtc agaggtttgt tcgactagat cttctttcaa aagagtggca
     2701 aacaattcaa gaccatactt taaagatgca ttgtcttgga tcgtgactgt ctccccatct
     2761 tcagatgtat accattctcc tgcctgttgc ataatcatcc ttactcgacc aaggtcagaa
     2821 ggattgactt cagcgatttt ctttcccgtc ttttctttga catctcgcgc tacttgaata
     2881 tactcgtccc acttcagatt ctccatatcc tcttctgtat aacctgcttc tgccatcaga
     2941 tccgtacgat agaagtttgc tgttacgccc gaatcgaaag gaacaccgta tattttatct
     3001 cctactttgt ttaccgcaaa tttatacggt gcaaaatgat cttcctttac aatagcagac
     3061 aaattagcga atgcatctgg ataagaagtc aaatagcctt gtatcctata atcttcaatc
     3121 aagacgatat tgggcaatcc ttctgtattc ccgctcgcta aagcggtgtt cagtttctga
     3181 acgatatcat cctgagacat cgtaacaact tctatctctg tttcttcgtt ctcataaact
     3241 ttttttgctt cattcactgc tttgatattg aatgtctcat cccacgccca gacagtgact
     3301 ttttcttctt tgtctgctgt actactagca cttccgcatc ctgttaagaa acctgatgct
     3361 gccgctaaac aaacgactcc agcaattaac tttctgatgt tcatttttat ccgctccttt
     3421 tccatccatt agttgtttac gtttgctaca ttcatcatac caaaatttga aaacggtttc
     3481 tttattattc agatacttca aaaatttctc ctaaaataat aaaaaaatac aaatcaacta
     3541 tgacaaaagg aataatctta gcatctagac caaagatcga aatggtgagg tttctttaaa
     3601 ataaaaatat ccttgcattt tctcctagaa ataagtattt ttaaattaca agactattct
     3661 taaggcgggt aaaaaaatgc caatacgcgt aattcttatc gatgatgaac agacgatttt
     3721 agatggaatg aaacagttga tcaattggtc tgagcacggg tttcaattaa tagggacttt
     3781 ttccagtccg ctgaaagcgc ttgatttttc tttggtacat gcaatcgata tcgtggtcac
     3841 agatgtaatg atgcctgaat taaatggaat cgaattatcg cggctattaa aacagcaaca
     3901 acctgaaaca cagatcctta tcctctctag ctatgatgaa tttgatatgg ttaaagattc
     3961 ttttaaagaa ggcgtcgctg attatctttt aaagcctcga ttgagtcctg atttttttct
     4021 aaattcctta actgccgttt cacaaaaaat caaaaaaaag cccaacaaaa gcccaatcac
     4081 caatcgttat gagcagatct gcgagaatct ttcacagcag attgctggac aaactcgtcc
     4141 attgcttgac cctcttcttt ttgaacacac cttgtttttc tgttatatac cgaaaaacgg
     4201 ccattcagtt caaaacaagg ctctaaacaa aaacagtcga tcacccattc attgatggat
     4261 agcgacgatt cgtttactat ttatcctgtt tctacctttt cggaagaatc cggctatgta
     4321 atcaatactg attccaaaga tacacttagg aagtttgttc aaaagtttcg tcaagaagtt
     4381 tttccaggtg atttttttgt tgtttctgat tcttttgcaa tccaagatat caatgaaata
     4441 ttccaagaga tcagaaattg caccaaagga caacgtttct atcataaggg aaagaacctt
     4501 gcttatcagc atgaacttct tccgttatta accaaccacg agcagcaagc aagtagttat
     4561 ttaacaaaaa tcgttcataa agactacgct ttagctgtca aagaacttga gaatttcttc
     4621 acgacgatat tggaacagcc tattgatcca gcttatttga aacatgaggc aatcaatcgt
     4681 ttctatgcat tactcaacgc tttggctgac gagcatgttg ggaatgaaga actttctttg
     4741 ctcaagatcg cccttccttc tttattagcc gatgctgagt atttcgaaga tttttctatt
     4801 cttgtcaaag aaacacttga gaaactcacc cacttttctg caaatgcaaa aaaagacaat
     4861 ttcgatatct tgactttgat ctacgagttc gtccaggaaa attatgagaa agaaatcaat
     4921 ttacaacttt tatccgaaaa atatcatttt tcatatagct atctctcagc catctttact
     4981 gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag tccgtatcag taaagccaag
     5041 ctctttttga cggaaacacg gcttactttg acggaaatct gtgagaaagt cggctatacc
     5101 gaactgggtt atttctccag agtttttaaa gaagagacag gaatgacacc ttctcaatac
     5161 cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga gcatgaactc tttgccaaac
     5221 ttctgttcat tttgttcatc ggtctttttc tgcaagccat cgtcatttct ttgtttattt
     5281 atcatcgatc aagggatgcc tatatccagc tgttcaatca atcgaatgat gtggtactaa
     5341 aaaagattca atctgaattt gaaagtttga atgatacaat cgagaataca ttagccgctt
     5401 ttgattctaa tccagctgtc aaaagctatt tttccaatga tccagcccaa catatggaac
     5461 agcttcagca actaagaaca atccaaaaaa tgaatgactc tctttcaaaa atccatccaa
     5521 tgatcgatta cgatgtattg atctttggag aaaatggaag aacttttgtc ggaaacgaca
     5581 tgctcactgc cgtatctgcc gattcttttt ttcaatctgc gattgctcag agagtgaatg
     5641 aacgtgctgc tgatacacaa atgctttttg cacaccacgg attgactctt cgcgataaga
     5701 aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac attgaatcac gtttacggat
     5761 atgctgtttt atcgatctca tccaagcaac tagcgaatct ttttcaatct gtagtaaatc
     5821 ctgagatatc gaagatctcc ttcatgaacg aagaacagac gatcatcgca tcaaatgaag
     5881 aaaatacaat tgggagccga tcaatgattt tcaaaaaact aacagtaggt gaaacaacga
     5941 tttcaaatgg acagcagttg acccgtcttc ctctgtaccg ccaggattgt acattactca
     6001 gtcaagtcga tcttcattct ttatctaacc aaatgggcgt gatttttccg attattttgt
     6061 ataatatcgt gacacttgct tttgctggct cattggtatt tatgtatttg gatcgacaca
     6121 caaaatcgat ctaccgcttg attgattcgc taaaacggct agaggaagat ggcttaaaag
     6181 ctactgttcc tgtacaagga agccatgaag tccaattgct agggaatacg atcaatcaat
     6241 tgctaagtaa tgctcagcat cattatgaac agacgatcca agacgaaaag aagaaacgtt
     6301 cgcttgaaat ccaaactatg caagcacaga tacagcccca ttttatttat aatacgctga
     6361 ctagcttgaa atttttgatt tggcagaaaa aaacagatga agcagttcaa ggaatcgaca
     6421 gtttcattac tttactaaga agtacgattg ggaaaaaaga agaagtgatt ccagtcaagg
     6481 aagaaatcaa aagcgtacaa agttacatcg atcttttgag tatgcgatat ggcgaaagaa
     6541 tctcggctaa aataatgatt cctgatgaac tgcagtcatt atctatgccg aatatgatcc
     6601 ttcaaccaat tatcgaaaac gcctatcttc atgcttttca caataaaacg caaggattca
     6661 tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt cgaagtgatt gataacggcg
     6721 atggcttcga tactgaaaat accaaacaaa aaaatgatca tttttcagga atcggcatca
     6781 gccatgtaga tgagcggata cgcctaatgt atggcgatgc atatggctta acgatccaat
     6841 cagtgattgg gacaggaacg actgtaacga tttgtctacc gcttcttcct aaaaatcaat
     6901 cagaatatca aaaataaaga atagccaagc gctgattttt ttattttaag ttaaataaat
     6961 caatcagtat tcttgacttt catctacgcg tctcttctac tgtcagcact ggataacagc
     7021 ccaatatttg actttctcct ccgagcatct tgatttcttg gagcgcattc tctactaatg
     7081 tcattggacg gtccaacaac aaatcgatca cgaaaaaata ttctcccaag ctagttttca
     7141 atggtcggga ttcaatctta cttaagttga tttcccgcca gccaaaagct gccagcattt
     7201 tatgaagcat ccctggccga ttagcaggta gtgtcaagat gacactcatt tttactggag
     7261 ctgactgctg gctggtcatc tttcggtcac cgacgatcca aaaacgtgtc tggttcaact
     7321 cattatcttg gatattctca gctaagatct ccaatccaac atgctgtgct gtttctaatg
     7381 aagcaatcgc tgctgcgtct tctttcggat gttctgctac atacattgct gcagcagttg
     7441 ttgattctgt tgctactaaa ggaacattag gataatgcgt ttctaaaaac tgttgtgatt
     7501 gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt tgttgctgga ttgcctaaaa
     7561 gctgctgctt gataggaaga acgatttcac ttttaacttc gacttctgga tgtttggaaa
     7621 gaagatcgat tgtgtgatga acagctcctt ccaatgaatt ctctacagga acgacagcca
     7681 gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg gatggaagca tacgcagtca
     7741 attgttctgt aggaaatagc tgacaagccg cctgataagt gaatgaactt tctggcccta
     7801 gatagctgac gttcatatgc ttttgatcct ttctataatg gtatcgatga tcttttctgg
     7861 tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct tcataccaag aaagtcgttg
     7921 tgccaataat gcttttattt cttctcttgt ttttccaaga gctaaaggtc gaatagacgt
     7981 ttcatcttgt tggatacgat caactagaca gtccgcttct gcctctaagt aaagaacaat
     8041 cggctgtgtt tttaaaaaac gccgattttc tgcactttgg acaatccctc caccagtcgc
     8101 gataattttg tcttcatttg acagctttct taaaatatct gactccactt ttcgaaagct
     8161 tttttcaccg taatagtgaa aatattcggc aatcgacaat tggatatgtt cctcaatctt
     8221 tgtatctaaa tctacatact ctttttgtaa acattgagct aaccctttac cgattgttgt
     8281 ttttccagcg cccatgaaac caatcagcaa aatgctagcc atgagcttct cctcctgaca
     8341 caagttctgc taaatcgtca aaaaatgccg gataagagat tgaaacagct tcagctcctt
     8401 ctagttcgca aggtccatca gctatcaaag cagctacttg cagcatcata ccgattcgat
     8461 gatcgcctct gctggacact tttgcgccat gcagaggtgt cggcccatgg atgatcagac
     8521 cgtctgctgt cgcttttatc tttgctccca ttttctgtaa ttcttctgcc accgcatcaa
     8581 ttctgttggt ttcttttact tttaattctt ctgcgtcttt gatgatcgtg attccttctg
     8641 cttgagtagc cactaaagct agaatgggca attcatcaat cagacgcgga atgatttctc
     8701 caccaatcgt tgctttcttc aaatgactcg tcttgacact taaatcagcc gattggttct
     8761 gttcattgtg attcgtctgg gtaatcgctg cgcccatctc ctccaacaca tctaagatac
     8821 cagttcttgt tggattgacc ccgacatttt ttaacaacag ttggctttcc ggaacaagaa
     8881 gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc tggaacagtt atctgttgac
     8941 cagttaattt ttgcggtccg gtcactatga tcgttttatc ttccactgtg atccttccgc
     9001 caaattgacg aatcatttct tccgtatggt tacgggaacg ttctttttca acgattacgg
     9061 atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga tttgacttgc gcactggcta
     9121 caggcatatt gtatcggata ggtgataaag agcgggttcc tgtcacagtg attggtagat
     9181 actggtcatt ttcttctcct tgcaagtcag cgcccatctc gcgtaaaggt tccatcactc
     9241 gccccattgg acgtttagac aacgaagcat ccccgaacag agtagtcgta aaaggctgtc
     9301 cagccaaaat acccatcaat aaacgtatgg tcgtaccgga gtttcccatg tcaattgatt
     9361 cggttggttg gacaaatgag tggctaccgc gaccatgaat cttgatcacc tcttcctctt
     9421 cttcgatctc gacacctaat tgttggaagg catgtaaagt actcagacaa tcttctgcgc
     9481 gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc tccaaacatg atgctgcgat
     9541 gggaaattga tttatctgct ggtatcctaa cagtccctct taatccatgt atttgttgta
     9601 ataattgcat ttatccagcc tcctatttga tccagcaatg ataactggta ttagacataa
     9661 tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa actgatttgc agtattccta
     9721 aaatatcttc cctggtctct tggatcttca aattaatcaa agaaactccc gcttttccga
     9781 tcagtccagt tacttctgca ataacgccag gttcatctgg cacatcgata aataaatcaa
     9841 aaaaggcagg aatcgcgcct tgcttatgga caggcaactg gtctcgactt tcttttgccc
     9901 gattgaaaaa agaaaagatt tccgattggt tctcagtcat gatccattct gctatttgtg
     9961 tcatttcttg ttgccaatct gccaatcgtt ccaataatat ctgcttattg ctcaacaaaa
    10021 tatctgtcca catgacagga tctgaggaag cgatcctagt gatatctcga aagcctcctg
    10081 ctgccagttg tttggctcgc ggatactctt gattgaattg ttctgcctga ttgactaagc
    10141 cagctgccag aatatgtgga aaatgactta gcattcctgt tatacggtca tgttctcttg
    10201 ctgtaagagt tacatatttt gctttggttc cgcaaaatag ttcttgcagt ttttcaacat
    10261 cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc attttcaaat agtttagcat
    10321 ctgctgcctg tatacctgat ttatgagaac cagccattgg atgtccaccg acaaaccgaa
    10381 gatttttctg ctcagcggat gtcacgactt gctgcttcgt actgctgaca tccgtaatga
    10441 tgacgtgatc agacaagttg attctttcaa ggacatcaag ataagctaag gttgtacgga
    10501 cgggaacggc taataagata acgtctgctt gttctgcacc tgtttcaaaa tcacttgcaa
    10561 cgtaatcaac gatctgctgt tctactgctt gctcacgggt ttttgactga ctatcccaac
    10621 caactactat cagttctgga ttggtctgac gaatacaaag tgccatagaa gcaccaatca
    10681 gtccaagccc cacgataaaa acatgttgtg ccatcttatt tgcccccttt ttagtaatgt
    10741 ttcaagtagt cacggtactc cttcactgca gctttcaact caacaagcga atcacttggg
    10801 aattttgaca atagctctgt cgctaccaca gtggctacaa cattttccgc aacgacgcta
    10861 gctgcaggaa ctgctgtact atccgaacgt tctacacttg ctttgtaagg ttcttttgta
    10921 tcgatatcaa cactttgaag tggcttgtac aatgtaggaa tgggtttcat gaccccgcga
    10981 atgacaagtg gttcgccatt ggtcattccg ccttcgaagc cgcctagatt attggtacgt
    11041 cggaaatagc ctctttcttc ttcccatagg atctcatcca tcacttctga gcctggtaat
    11101 gaaccagaag tgaatccgac cccaaattct acgcctttaa aggcattgat actaaccaca
    11161 gcttgtgcga ttttggcatc tagcttcgtg tcccattgta cgtagcttcc taatccagct
    11221 ggaacgcctc caacgagcac ttctacaatt cctccgatcg tatctccatt tttctttgtc
    11281 tgatcgatca aatcttttat tctttgctca actgctggat caacaactcg tacttctgac
    11341 gcttcagaaa tttctttgat ttcttgaatc gagatatttc caggtatttc tgcttggatt
    11401 ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa gttcttccag aattttcttg
    11461 gcaattgcgc cgattgctac acgcatggtc gtctctcttg cagaggaccg ttcaagcaca
    11521 ttccgaagat ctcgatgatg gtatttcata ccgcctacca aatccgcgtg tcctggacgc
    11581 ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca cttctgctac agacatgaca
    11641 gttgtccaat ttttccaatc tttattttct accactaaag tgacaggtga accaattgtt
    11701 ttgccgtgac ggatgccaga cgtgatccga acacgatctt tttcgatctt catccttcct
    11761 cctctgccgt aaccagcttg tctgcgagcg agttcgtgat tgatttcttc ttctgacaga
    11821 ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt ctggtccatg ggattctccc
    11881 gcagtcatat aacgcatctt acagtcctcc taagtaatct ttgaatgtct ctgtgggaac
    11941 aggaacgatt tttgcttgcc caatcttttc tagcaagatg atattcaatt ggcttcctct
    12001 tgctttctta tcatgggtta tcgcgtggaa taattcatct ggatgactta gatcgtacgt
    12061 aatcggcaga tggaattttt gaagtatcct ttctaaagca gctgtcgatc cagttggaga
    12121 caagccgatt ttttctgcat gagaagtgat ggctaccatt ccaatagcta ctccttctcc
    12181 atgactgact accccatatc ctgctgtatt ttcaattgca tggccaattg tatgaccaaa
    12241 atttaaaata agtcgatttc cttggtcaaa gacatcttct tccactactt ttcgtttgat
    12301 ttccaacgta gcagtaatga ttggaacagc atgttccaga agatcttttt cgtctttcag
    12361 ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct gctgatttga tgatttcagc
    12421 gattccttct cgtaaacgac gtaccggcaa agtttttaga acctcaggat caatcaatac
    12481 accagctggc tggctaaatg ttccgacaag gtttttcgct ttgtttgtat tgactgctgt
    12541 cttcccgcca atactagaat ctacttgtgc aagtagcgta gtcggaattt gaacgaaatg
    12601 gatcccacgc atgaacgtcg aggccacgaa tgctgctaga tcacccacca ttcctccacc
    12661 aagtgcaagt atcccatcgc tgcgggtaaa atcattatct gctaaaaaat cgtataactc
    12721 agctgcttgt tctaaagatt tactggtctc acccgccggt acgattgaaa ggactggttc
    12781 aaaattagct tgtctcaatt ggaacatgat tttttctgca tatagtggag caacgtttgt
    12841 atcagtgatc acagctactt tttgaggtct ccaaatcgtt tgaagccatg atccagcctg
    12901 ttctagtgcg cctttttcaa tatgaatatc gtatttttgg ttatcaacat gtacagtaag
    12961 cattcgtttc acttctttct tttaaaaagc tggaattaat cgagtgtctg aatcttttcc
    13021 attgcttctt tcatgatcgc aacttctttc atcatgcgtg agtaagtctt ttcattcaac
    13081 gattggggac cgtctgacca agcatttgca ggatcaggat ggatctctac gatcatgcca
    13141 tctgctccgc ttgccacacc cgcacgagcc attggtggta caagttccca aattcctgta
    13201 ccatgacttg gatcaacaat cacagggaaa tgagttaatt ttttcatcaa tgggacagca
    13261 cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc gaatcccacg ttcgataaaa
    13321 atgacaggag atttgtcttc gactgcaatg tattctgcag cattaagcca ttcattgatc
    13381 gtaccggaaa ttccacgttt caatccaaca ggtttacctg ttttaccgac agcagatagc
    13441 agtttaaaat tttgcatatt acgtgcgcca atctgtagga tatcgctgta ctcagccacc
    13501 atatcgatat gcccttcatc catcacttca gtaatcactt tcattccaaa ttcatcagcc
    13561 gcttgacgga tgtatttcaa tccttcttct tctaaacctt ggaaagcata tggagaagta
    13621 cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc cggctttggc cattcgcgca
    13681 cattctctga tttggtctaa tccttcgata gaacaaggac cagccatcgt cacgaaactg
    13741 ccgtcaccaa tttttactcc gtctacatcc acgactgtat tgccaggatg gaattctcga
    13801 gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt ctactccttc atagcgtgta
    13861 aatgctcctt cttgtaaact tcgcgtatca ccttttagtc caatcactac tcgttctgtt
    13921 ccttgatcga ttgagacttt gaatccttct ttttcgatat gatcgacgac tttttttatt
    13981 tgttcttccg ttgcttcttt tttcataatt actatcattg tttttcccgc tttcttttgt
    14041 ttacttatct atcttggctg attttctcta ctttttcaag gtggttttac attaattcct
    14101 actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt ctttcctact tatgacccag
    14161 ataatcaaca aaaaaagatg tccctgtagg aaattgcctc tacagtgaac atcttcaacg
    14221 agccttaaaa agcgctgcaa aaatataaaa tagccactat agatgaattt ctctacgtgg
    14281 ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac aaacctggtt tgtttgtacc
    14341 cacgaagtca tgaatacaaa cctatctaaa atagctaaag taatacgtat tcagttttgt
    14401 aagttttcta gatttcataa aaaatcgctc caaactga
//
LOCUS       DAZBSA010000070        14373 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000070 DAZBSA010000000
VERSION     DAZBSA010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.070"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(553..1737)
                     /locus_tag="ACHGMT_001971"
     CDS             complement(553..1737)
                     /locus_tag="ACHGMT_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFN6835706.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(1762..2769)
                     /locus_tag="ACHGMT_001972"
     CDS             complement(1762..2769)
                     /locus_tag="ACHGMT_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN6835707.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(2941..3300)
                     /gene="comGG"
                     /locus_tag="ACHGMT_001973"
     CDS             complement(2941..3300)
                     /gene="comGG"
                     /locus_tag="ACHGMT_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFN6835708.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(3272..3697)
                     /gene="comGF"
                     /locus_tag="ACHGMT_001974"
     CDS             complement(3272..3697)
                     /gene="comGF"
                     /locus_tag="ACHGMT_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFN6835709.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            complement(3660..4007)
                     /locus_tag="ACHGMT_001975"
     CDS             complement(3660..4007)
                     /locus_tag="ACHGMT_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835710.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            complement(3979..4449)
                     /gene="comGD"
                     /locus_tag="ACHGMT_001976"
     CDS             complement(3979..4449)
                     /gene="comGD"
                     /locus_tag="ACHGMT_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFN6835711.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(4424..4702)
                     /gene="comGC"
                     /locus_tag="ACHGMT_001977"
     CDS             complement(4424..4702)
                     /gene="comGC"
                     /locus_tag="ACHGMT_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFN6835712.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(4699..5748)
                     /gene="comGB"
                     /locus_tag="ACHGMT_001978"
     CDS             complement(4699..5748)
                     /gene="comGB"
                     /locus_tag="ACHGMT_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFN6835713.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(5705..6634)
                     /gene="comGA"
                     /locus_tag="ACHGMT_001979"
     CDS             complement(5705..6634)
                     /gene="comGA"
                     /locus_tag="ACHGMT_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFN6835714.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            6766..8520
                     /gene="ade"
                     /locus_tag="ACHGMT_001980"
     CDS             6766..8520
                     /gene="ade"
                     /locus_tag="ACHGMT_001980"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFN6835715.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(8618..9280)
                     /gene="trmB"
                     /locus_tag="ACHGMT_001981"
     CDS             complement(8618..9280)
                     /gene="trmB"
                     /locus_tag="ACHGMT_001981"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFN6835716.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(9350..10132)
                     /locus_tag="ACHGMT_001982"
     CDS             complement(9350..10132)
                     /locus_tag="ACHGMT_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFN6835717.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(10336..11547)
                     /locus_tag="ACHGMT_001983"
     CDS             complement(10336..11547)
                     /locus_tag="ACHGMT_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6835718.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(11540..12277)
                     /locus_tag="ACHGMT_001984"
     CDS             complement(11540..12277)
                     /locus_tag="ACHGMT_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6835719.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            12415..12849
                     /locus_tag="ACHGMT_001985"
     CDS             12415..12849
                     /locus_tag="ACHGMT_001985"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFN6835720.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            12842..13186
                     /locus_tag="ACHGMT_001986"
     CDS             12842..13186
                     /locus_tag="ACHGMT_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFN6835721.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            13366..>14373
                     /locus_tag="ACHGMT_001987"
     CDS             13366..>14373
                     /locus_tag="ACHGMT_001987"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFN6835722.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
BASE COUNT         4573 a         2929 c         2422 g         4449 t
ORIGIN      
        1 tgacgttttg gtcgactaaa ttttaccaaa agtgagtctc tttttgagtt gaaacgaaga
       61 aaaaagtgtt agtagaaaat cgctttcgat tttccaccaa cacaaaatat tatagaaccg
      121 ttttttcgca acaaaaaact tgattttcat acctgtgctt tcacgattca ggcaaagtac
      181 aggtggaaaa tcaagttttt attattcgat caatgaatgt ttcggatcgg ttttctagct
      241 aaacataaag cgaagaataa agtcgacagc ccacgaggct gatctaaaaa tgttttttct
      301 ttttacatat gtaatgtccc ttataaagat gaatgaaaat aaaatattgg gattttcctg
      361 aaaatcaata aataaaatgc cagtcttttc ccttttgacc acttttccaa gcaaaattcc
      421 ctaagaagat cctgtaaaaa agaagggaaa ttgaagttaa attaccattt tgcggtcttc
      481 tccgcaaaaa aaacacatgc aagaattccc tcttgcatgc gtttcatctt ttatcctaac
      541 gaatgctttg gcttagccgc gcagacgctc cacatcacgg gcaatcatta gttcctcatc
      601 tgttgggatc actaatactc ttacttttga ttcttttgta gagatatcta cttcttcacc
      661 acgcaattca ttcaacgctg gatcgatttc acaaccaaac caagacatac ctttgatgat
      721 ttcactacgt acatgtccgt cattctcgcc gataccagca gtaaagacaa ttgcatcaac
      781 accattcatc gttgtcacat aaccgccgat gtattttcgg atgcggtcca cgaaaatgtc
      841 ataagctaaa cgaatatgtt ctttttcata gttcttttca agatcacgca tatcactaga
      901 caatcctgtt aggcctagca gaccagattt cttgttcaaa acattgatca tttcatcaat
      961 atcaatctct aatttttcca ttaaatatgg gagaacagct ggatcgatat cacctgaacg
     1021 tgtacccatt gtcaccccag ctaatggtgt gaatcccata gaagtatcca tggatttacc
     1081 tccatcgact gctgtgatcg atacaccatt tcctaagtgg caagtgatga tttttagttc
     1141 ttcgatcggt ttgttcaata attttgccgc acgttccgac acataacgat gacttgtgcc
     1201 atggaagcca tattttctta ctttgaaatc ttcataataa gacaccggta agctatataa
     1261 ataattttgc tttggcattg ttgagtggaa agatgtatcg aatacagcca cactcaaaat
     1321 gtcaggtaaa atctttttaa acgctttgat ccccattaag tttgcaggat tatgcaaagg
     1381 agcaaattct gctaattcag caatgcggtc cattactttc tcatcgatca ctactgaatc
     1441 cccataagtt tctcctccgt gaacaacacg gtgccctact ccggtgattt catcataaga
     1501 gcttaaaatt tgcagttcaa tcaactgatc taacagcatt tttactgcta cttcgtggtt
     1561 ttcgatatcc atgatttgtt cgaatttttg attatcgccg tacttgatgg taaaaataga
     1621 atcttgtaaa ccgatacgtt cgacgatccc tttcgcaact acggtttctt ctggcatttg
     1681 atataattgc catttcaaac ttgaactccc agcattgatt gcaattgttt tagacatatg
     1741 actctccttc tcgttgaatg attacagagc agatgatttc cagtttttaa actcgttgaa
     1801 aaattccgtc accttctctg gttcttttaa tgaagcaagt ttcacaagca agacttcttt
     1861 tacttgttgt gcttgtggtc cttttttctg taagatcaaa atactttttt gcgattgttt
     1921 attacggaac aattcatctg gcaattgaat cattccttga agataacctt cttctttaaa
     1981 ccattttttg atttcttcac tttgatccgt ttctaaaaat ccactcggca tcaaaaacaa
     2041 accaaaacca tccggcttca catatttcat tgattgctct agcaataaat gatgtgcata
     2101 actatggcct tcttcggtac ttgttaaaaa ctcttttgct ttttcgtcat tcggataata
     2161 accgattggc aaatcgctga ttgcaaaatc gacaggatct attaataatt cctgcaaccc
     2221 gtcttgatga aagtattgaa catttgcttg tgtcaaatca cttgtagaag ctgcgacagc
     2281 caacaatgta tcatcaatat ccacaccaat tccttctgtt tggtaccccg cattcgatag
     2341 gtttaaaaga actgttaata acaagttgcc cattcctgcc gcaatatcca gaatcttaac
     2401 cggtgctttt ttatttgtgt atagttgttc aataagaaaa acaaataaaa aaccgatact
     2461 atcaggagtg agttgatggt tcgcctgcaa atgttctgtc tggctgccct tcaataaaag
     2521 taattgagac agtctgcgcc attcttctgg ctcgaaagaa agtttcttca actcttcata
     2581 aagagctgtg atccgttgcg ttgtctcttt tgtcggcact ccatccacca cacgtacttg
     2641 ataatcatct attaaatttt ctgcattttc gacataggct tctaaaaagg aagttcccaa
     2701 tgcattctgt aaaagctgaa tagcttccag gttttgatta aacccttgtt ccattttttc
     2761 tggtaacatg gtgcacctca tttctagata tattataaca attttttcac ctgtttaatt
     2821 ctatcgaaca attgacaaaa tttcaatcgt tttcactgtt ttttatttgt gaaacggcaa
     2881 acattttttt attaaaaaca gtgttataac agaaaagagg gcgttttcac gaacattcca
     2941 tcattctgta ctagtatctg tatcattttt tacttcttct gtgaaccgaa agataaacgg
     3001 tgagacttcc acctctaaga atagggactg ttgtacccac tgatatgtta cagatccagt
     3061 tgtgtagtaa aaaactcctt ccgaactttc ggcaagcgta gactgttccg ataaagccat
     3121 ctgcttcatt atttttgcta tataataatt tttgattcgt atattaagat ccattgacaa
     3181 acggtagctt tctaatgctg ccatcaataa aaacgtacat aaaagaaata aaagcaagac
     3241 agaaaataaa atagagccct tataacattt tctactgatc gttttttgtc catatggcat
     3301 atccatcttc tccattttca aagtgtacat aaaaagaaat tgtgtgttct ttttccaata
     3361 attgaagctc agtgatactt gttaacatag gttgatgtcc gccggaagtt cttcttttac
     3421 gtatcattgc ctgatatctt tcgatgatat actgtttatc ctcgatcgtg tagtgcagtt
     3481 gctgattttt cacagagaca ttgtgactgc tcttgagttc ttcttctaat tgtgctaaaa
     3541 atattagcca ctctttttga tctttacgct gcaaaaattc acttacttgg atggtttgct
     3601 gaatcaaaag attgaatgtg agcaatagac tgctcaatat catcagtcca attagacatt
     3661 caatcagcgt aaatgctgga attacttgtt ttttcaatac tgatcgcttc tttctccata
     3721 ttgacaaaca ctttttcgat tactccatta ttatcgacaa tttggatatc gtatgggatc
     3781 atttcttccc ttcgctctgg tcttttgtga tacatccgct gcgtcttgat atcttcataa
     3841 atcattcgaa taaatagcag ttcctcccca cttttctttt ctgcctgaag caaaaacagc
     3901 tggccaaagc tcactaagta gacacagaca gaagcgatca taagcgctgt aagggactcc
     3961 aacaaaacaa atcctgtttt attcaattct ttttgtatag cgaccactcc ctatctggaa
     4021 ttgatacgta atcgtccgct ctttttcttc ccaataaaat tgataagctt ttagtgaact
     4081 ttcattcccg gttctagcag caaaagtaat agaagcatgg cgtttcaagg taatctcatc
     4141 aggaatatct aatacttccc aatttatttt atctggattc ggaacatgaa aaatgatttg
     4201 attctcttca ctattccaat aaaagcctgt ttggatctga ttcacaatag cgattttttg
     4261 cgtagaatag attcgttttt caaattggcc gaaaaattga tagactgcca attcattctt
     4321 ccatgcagaa aatgcgagaa ctggaaaaga aaggatcgtt gtaagtaata aaatcagtaa
     4381 gagtgattca aacaacgtat atcctgatgc taaaacctta ccttcaaggc cgctgataga
     4441 tttctgcatg ttctcgtttg atatagcctt cattcaacaa ttcctccaca gtaggaactc
     4501 ttccattatt ttgaagcgca tagagctctt tttgtgtgtc taccaactgg atgatcgctt
     4561 cacgactttc ttggtctaca tgattcctat aacgcgacaa attagggacg aacaacaaca
     4621 ctaatacact gatgatcagt aagacgatta gcatttcgat caatgtaaaa ccgctataat
     4681 aagatatttt tctctttttc atggcaaaac ctcctctatg cttccgtaaa caggtaataa
     4741 aacagcggca taaactaata aaatcaaagc cgctacgccc aaaaaaatga ttggctgaat
     4801 aaaatgcatc cagtgatgaa gacgtttgaa aagctgtttt cttgtccatt cgctgtaaaa
     4861 aatcagttct ttacctaaac ttccctttgc ctctccctgt aaaacaattt tactgaattc
     4921 ttctgataga aaacgataat tctggaactg ctcagccaat gacatacctg cctccaatcc
     4981 ttgaatcaac ttagaggcaa gatgctgaat cagactttct tgtttcgttt cctttaagca
     5041 gactatgatt tgccttaatt cgaaaccttc atgaaaaagc tttccaatct ctagtgacaa
     5101 ataagccgat tgatacaagg aatatatcgg tccaatcaat aattgtctgc ttatccattc
     5161 actctgatca acaggagaca acttcatcag acgatacctt actagtaaag ccaatagagt
     5221 aaccgctagg atgaatagta gaacatacca atgaaagact tgtatcagct gaatacccca
     5281 atgctcttgc atgaccattc ctgaaccaat aagctgaggc aaaacgaaca accgcatact
     5341 aaacaagata cctagcaaaa aggtgagcaa tagcatcgga tatgcaagca gctttttcaa
     5401 atcattgcga aatccttcaa caagccgaaa ctgtttggca atttccaaca aagttgtcgg
     5461 cagattccca tgacgttcag ctaactcgat ctgtaccagt tgctctgctc catatcctat
     5521 ttgcgaaagt acctcactaa acgcttgccc ttcctttagc tgatgttgga tatttgccaa
     5581 aataacaggt ggaaacgaac ggatcgtgag taataaataa agggattctt gcagactaaa
     5641 accagataaa agcagttctc ccatggtttg tgcaaactgg ttttgctggc ttcttgttaa
     5701 tcctttagaa tagttctgac tgccaaatat tttcctgagc ttcttcataa ctttcctccc
     5761 atgatttttt tactgattct tcctccataa attgataact ccataaaaca gaaggatggt
     5821 gattggcatc ttccaaaagt tcttgataaa tgacaccagc taaacattca gatagctctt
     5881 cttttttctt tgtcagttca tggattctga ccaatgtacc ttcaagattg cgtgcatgta
     5941 cggttgcaaa aacccggtgc cctgttagtg ctgctcttac cgcagcatgt gcagttgatt
     6001 catctcttat ctcgccaatg attagcaagt ctggacgatg ccgtaatgcg agtctcaaaa
     6061 gtgtatcata agtctgtgct attttcggat tgatctgtag ctgcagaaaa aacggttctt
     6121 ctatctctac aggatcttcg atagtaatca cttgcaattt ttcttccaaa gccaaccgat
     6181 acattagaga agtcttgcct gatccgactg gtccgctgaa aaggtataat ccacgtctga
     6241 cggttttctg taaaatagtt gcaaaatctc gagaactaaa gcaacgatgg ggtgtttcaa
     6301 aagtaccgta taatagccga atcactaaac tctctcgttg aaggtaatct cctacagaag
     6361 acaaccgcag acgaacttcc tgtttttcta aagaataagt aaatgcacct aactgagctt
     6421 ttcttttttc tccgatatcc atctgtccca aatacttaaa tcttgagatc agttgctgtc
     6481 catctgccga agagactttc ttccagaatg ttcgtttttt tccatagcga aaatagatgt
     6541 aaaatacttc tgaagctggc aatatatata cgtcttgggc atgtttggca aaactttcac
     6601 ttatcatttg atccgataat cgttttattt ccattattct cccctccaca aaaaacatac
     6661 gtctttttga tctttttgtt tcaaataaaa tgaatttaca aaaaaatatt ggcaaaaacc
     6721 gaatttatgt tatacttata aaaatttatc ttgggaggca ctccattgga aacaacatta
     6781 aggaaatata tcgaagtagc aagtaaaaaa aagcctgctg acctagtgat caagcatgct
     6841 aagatcgtca acgtctttac gaaagaaatc atggaaaaag atgtagcaat ctgcgaagga
     6901 atgatcgtag gaatcggtga atacgaagga aaaagcgtac ataatgctaa tgggcaatat
     6961 ctagtgccag gattcatcga tggccatgtg catatcgaaa gctcactttt atcaccaagc
     7021 gaattttcaa aagtatcctt gcttcatgga gttactactg tagtgacaga tccccatgaa
     7081 ataggaaaca ttgctgggag tacaggtctt gagtttatga tagaagatgc aagacaaaca
     7141 ctcatgaata tcttttttat gctaccttct tgtgtacctg taaccccttt tgagaccaat
     7201 ggtgcaaatt taaatgcaaa aaaattagcc ccttttttac aaaaacccga agttttaggc
     7261 ttagctgaag tcatgaacta tcaagcagta gcaaataatg aaacagacat cctagaaaag
     7321 atccaattga tgcaccagca taagaaaaaa attgacgggc atgcagctgg tatcggcatg
     7381 gaagagttaa atgtttatcc tgcagcaggt attcggacgg atcatgaagc tacaactgca
     7441 aaagaagcta aagaacgttt ggatcttggt atgtatttaa tggttcgaga agggaccgtt
     7501 gcaaaagatc tggcatcgct actaccggcg atcacacctg aaaattcaag aagatgcttc
     7561 tttgtcaccg atgataaact gatcaatgat ttagtaaaag aaggatcaat cgatcatatc
     7621 gtccgtcaag ctattgcact tggaatcgat ccattacaag ccattcagat ggcaacattg
     7681 aatgcagcag aatgttttga tttgaaaaca ctaggtgcga ttgcccctgg gtatcaagct
     7741 gatttcttct taacagacga tcttaccaag ctgccaattc atgaagtttt ctataaggga
     7801 acattagttg ttcaatctgg gcaactccaa gaccatttat ttgctgatca ttcaaattca
     7861 tatacgtgtt gtctgccaaa actaaacgct caaccattaa atccacattc gttagagctg
     7921 cttctttctt cttcacaagc ccatgtgatc gaaattatcc cgaatagtct agtgactaaa
     7981 gatttggtag aaaaagtgga ccgacaaggc gaacgattca ttccttcaat cgaaaaagac
     8041 caattaaaga ttgcggtgat cgagcgtcac catcaaacgg gaaacattgg attaggcata
     8101 gtgaaaggat ttcagctgaa acaaggagcg atagcgacaa cgattgccca tgactcgcat
     8161 aatctagttg tcgttgggac aaacgacgag gatatgctgt atgctgctaa tcttttgatt
     8221 caaaaaggtg gcgggatgac cgttgtcaac aatcagcagg aaatagcttg tgtttctctt
     8281 cctataggcg gaatcatgac acaggaacct ttttcgatcg tctatgaaca gttgaatgaa
     8341 ttaataacta aagcacatca actaggagca gctaaaacct ttgatccttt tttgacactt
     8401 tctttcttaa cattatctgt catacccgaa ttgaagatca ccgacaaagg attattttca
     8461 ttttctcgct tccaattgat tccaccttgt ggaaaaaccg atgaaataaa tacattttaa
     8521 atcaaataaa tcccagaaaa tacattacat ctgtaatgtt cattttctgg gatttattta
     8581 tggatattaa tgtttttatg aaagcagaaa gttagtttca cagacttttc tttttggtct
     8641 tatcttgaaa ttgagcttct actcgataaa tacgttgacc ttttgaagaa aatttttctt
     8701 catactccgt cataatattg ccttcaaatt gactttgatg taaatctaac catacctgtt
     8761 tgaggatcat gccatattga gaaaaacttg ccagagaata ctcaaacaat ccttgattat
     8821 ctgttttgaa atggatttct ccatttggac gaagaatctg ttcatctaca gcaagaaaag
     8881 tcttatatgt caatcgccgt ttttcatgac gtttttttgg ccatggatcg gaaaaattca
     8941 gatagatttg atctacttcg ctgtcagcga aatactctgt caacgctgat ccatccacat
     9001 gtagaagttt caggttagga agcggttgtt cgatcaactt gtctagtgca atcgaaacaa
     9061 cactgacctg catttctact ccaatataat tgatttcagg atgggctttt gccattccag
     9121 tgatgaactg gcctttcccc ataccaatct caatgtggat tggatgatcg ttttcaaaca
     9181 attcattcca ttttcccttc cacaatgttg gatcactaat tacaaaattc gggtgagccg
     9241 ctaacatttc cgctgctccc ggacgatttc ggacacgcat tatttatctc cattcctttc
     9301 ctctaattaa aaaggtctgg ccaaatctcc gcagagtttg ggccaaacct cagccactga
     9361 aaattcgttt taattgtaaa atttcttcgt tcatccgtcg atgatcttca cgtttgcaat
     9421 atctggtgat ttcttggagc aaattcatgg ccgcatacca atgaaatttt tcaagcattt
     9481 cgtcagtcgg gcgtatgcca taactgatca gccactgatt ccagctagac agtggcacat
     9541 aatttcctaa aatcgttgca atatccaccg cagggtctgc aaacatgatt gaatcccaat
     9601 cgactagata taaataattt ttacatacta accaattacg atggtttaca tctccgtgta
     9661 ctactgataa gttttcctta tctattgacg ggatctgctc acgaagaaaa cgatggacaa
     9721 tttgcagaaa ttgatttttc tggagttctt ccggtaattc tttttcatat ttttgcagca
     9781 tcttctccgg cgtcatgaca cgaccaccga tacgggtcag catggtttta agagaatttg
     9841 aatgatgtaa atggtacaaa acatcaatca cgtcgtttct ttgaccaatc tcattaggag
     9901 tcagcaagcg accttccagc cactcttgtg ctgtcaaagt atcgccattc cccgttcgtt
     9961 ttgtccaaac aagttttggt gtaattcctt cccttgaaag agcagccaac atcggagtcg
    10021 tgtttcgttt aataaagact ttgtcctcat cacgcatgcc tatgtatgtt tttcctgtgt
    10081 cgcctttgat gggacgtaac cgccactctt ggtctaactg aaactccatc atgcattctc
    10141 ccgtcctctt actctttgtt ttgcagtact tattattcta gtcgcctgaa cactacacgt
    10201 caagcacata tacgttcttt ttcatttaat tgccattcaa aaaaccagct tttctcaaaa
    10261 aaaatagaac gaacagcatt cttactgtca acgctctatt tttcattctc atttttaccc
    10321 tacacagctt tgttttcagt ctgacatctt cttcaaacga gttggtacat atagtttaac
    10381 aaagagcata gtaacaatca aataagccgc tagcggaacc catcgctgct gcccatctaa
    10441 tacgactgca gcaatcaaac caaaaataac cgacgcgata attaataccc atgtgagtag
    10501 atactgaatc gcctgcttct tctgattctt atggattgga taaagctgaa cgagaaccat
    10561 atagcggaac tggttcgcta aaggtaacag ttggaaaccg atcaaatata aaaataagaa
    10621 accgataatc agactaaaat aaaagtcgac tactccggca atcaataacc cacctataat
    10681 tactaatcgg aaataaagac cactaaaatc agaacctctt gccaatcttc ttgcaaacaa
    10741 gtaaagataa gtgttttttg attctctttt aatatgagtc aatataggat ccaaatagcg
    10801 tcttcttttg atctttgccg taatttcagg aacatctgtg aataaattga taaattgata
    10861 gatctgatgc aacctatgtt cttctacatg caccattttc tcccaatcca atcctttttc
    10921 ttgttgcttc cacaaaaacc agtaatatag acttgcctgt actaatgcac ctgctattcc
    10981 tgcccatatt agccaataaa atgatagaaa caaaaagatc agagaagaaa aaaaccagcc
    11041 taatttccaa taacgaataa cttgttgatc tagtcgaaac aattctgctc tcttgatttg
    11101 cagatgactg aatttcaaac tccataatat gatcagatag acgaaaaaca tcgaaaagga
    11161 ttgctccgta ctaaccacca ctaatggcat ggccaatccg catgccaata tcaaaaagat
    11221 caacggaaaa atgcaagaat agcgaaaagc tcgttccaaa taggtttgca tttctttttc
    11281 tttaggaagt aaaaaaacaa tatctgctgc ctctgcaaaa gtagccatct tacccacgaa
    11341 taaacagagc atccaaaata cagagattat gagccctcca attgcaaacg gtggctgcag
    11401 ggttttcaac caattagaat aatataatcc cacaccgcca atcaagaaaa gacaaactaa
    11461 aacaaaatga tcgttcaata cataacgcat atatttcatc attttctttt gatgtcttgc
    11521 taaacgtttt tgaaaaaaac tactcatgtg cctgctcctt tgttaaagca atatagattt
    11581 cgtccaaaga agaatccggc agctgaaatt cttttctcaa catctcaaga gaaccgtctg
    11641 ctcgtatctc tccgttatgt aagaccacaa aacgatcaca atatttttct gcagtagcta
    11701 aaatatgcgt agacatcaaa ataccggctc cttctttttt catttctacc atcaattcaa
    11761 gcaatgcatt gatagctagt ggatccaagc ctaaaaatgg ttcgtcgatg atatataaag
    11821 aaggctcgat caaaaaggcg cataagatca tcactttctg tttcattccc tttgaaaaat
    11881 tagctgggaa ccagtcaaga cgattagata accgaaaagt ctccaacaac ttttctgctc
    11941 ttttcattgc tatatccata ggtatatcgt aggccatcgc agtgacttcg atatgttctc
    12001 tcaatgtcaa ttcttcatat aaagaaggag tctctggaat atacccaatt tgtttgcgat
    12061 attcttccgt atttttcttt aaggatagat gattgatttc tatcgttcct tttgaagctt
    12121 ccagcagacc gataatgtgc ttgatggtcg tacttttccc tgctccgttc agtccaatca
    12181 accctactat ctctccaggc tgtacatcga aactgatatc ttttaacaca gggagatgtc
    12241 cgtacccccc tgtgacatga tcgataatta aactcattct ttttcctccg ccatttattt
    12301 ttatttatta taccacgaag aaaatgacgg tattttgttt ttttccttac aatagcttta
    12361 acttatggta aagttaagct gaagtattta ttctttatag aaaggatgtt ttatatggaa
    12421 aactgtattt tttgcaaaat catcaatcaa gaaatcccaa gctataaaat atatgaagac
    12481 gacaaggtct atgctttttt agacatctct caagcaacga aaggacacac attagttgtg
    12541 cctaaacgtc atgtagcaga tatttttgaa tatgatcccg aacttgcagg agaggttttt
    12601 tcgcgtgtgc ctaagattgc tcaagcattg gaaaaagctt ttccagaaat gaaaggtctt
    12661 aatatcttga ataacaaccg tgaactagcc tatcaatctg tttttcattc acatatccat
    12721 ttagtccctc gctacagcaa agaggatgat ttttctattc actttgtcaa tcatcaagat
    12781 agttatggat cggaagaact aaaagctatt caagaaacaa tcgtaaagca ggtgtcctgc
    12841 gatgattaaa aactttttca aaggactgtt atttggagct gctgctggca ctgtcggagg
    12901 tctgttattg gcgcccagga gcgggaatga aacaagaaag aaactgatcg aagaattgga
    12961 agaagcaaca actctgacaa atgatttaaa taacagcctg aatcatttta aaagcgcatt
    13021 gatcgaaaca aaagaaacag ctgaatctgt gattcctgct ttccaagaag caatcaaaaa
    13081 agatatcgaa gattttaaat tccaagccga accacgtatc acacaaatca atgaacaagt
    13141 agaaaaacta aaagctgatc tgccagagta tcctgaacaa atataataat gattattttc
    13201 tcaacagatt taactatata agtgagaaaa gtttgcgtat ttcctaggaa tgtgatgaaa
    13261 ttttgaattt tgtttgaaac taccacgaca acgtttttaa aggaaatggg atatggtata
    13321 gttaatatat aaaaataata actaattaca ggagtgcaaa ttttaatgaa aaaaaaatca
    13381 attattttgg cagctactag tgcgttggct gttttaactc tagcagcttg ttcaggcgat
    13441 actaataaag atatcgcaac gatgaaaggc ggaacgatca ctgtatctga tttctatgat
    13501 gaagcaaaac ttgaatcatc gaaccaatcc ttagtacaac ggatgatcat ttataaagta
    13561 ttcaataaca aatatggcga taaagtaacc gataagcaag tggatgcaga atacgacaaa
    13621 caagcgaaat cattaggtga tacttttgaa tcacaattag aagctgctgg atatacgaaa
    13681 gatacttata aagaatatat tcgtaacaat ttagcttttg aagctggctt gaaagctcac
    13741 gtagatatta cagatgacga tttgaaaact gcttggaaat ctttccatcc agaagtagaa
    13801 gcacaaatca tcaaattatc aagtgaagat gaagcaaaag atgtgaaaaa atcagcggat
    13861 gatggagatg atttctctaa attagccaaa gacaaatcaa ctgatacaga gacaaaagaa
    13921 gatggcggta aagttaaatt cgactcaacg acaacaacta ttcctgcaga agtaaaagaa
    13981 gcagctttca aattgaaaga cggtgagatc tccgatgtta ttactacaac aaatcctact
    14041 tcttatgcga cagaatacta cgttgtcaaa atggtgaaaa accaaaataa aggcaatgac
    14101 atggataaat acaaagatca actaaaagat atcgctactg aaactaaact aagtgacaat
    14161 gcattcacaa ctaaagtaat cggtgaagaa ttaaaagatg caaatgtaaa aatcaaagat
    14221 gatgcctttg aaaatgtgtt gagtgcattc acaacaactt catcaagtac gaaagactca
    14281 agtgaaacta cagcttcaac aaagagttct gacacaaaat caactgattc aacaaaagaa
    14341 tcttcaactg aagaaacaac tgattcatca aaa
//
LOCUS       DAZBSA010000071        14129 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000071 DAZBSA010000000
VERSION     DAZBSA010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.071"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..3694)
                     /gene="rpoC"
                     /locus_tag="ACHGMT_001988"
     CDS             complement(41..3694)
                     /gene="rpoC"
                     /locus_tag="ACHGMT_001988"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFN6835723.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="ACHGMT_001989"
     CDS             complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="ACHGMT_001989"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFN6835724.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8118..9086
                     /locus_tag="ACHGMT_001990"
     CDS             8118..9086
                     /locus_tag="ACHGMT_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN6835725.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9104..10009
                     /locus_tag="ACHGMT_001991"
     CDS             9104..10009
                     /locus_tag="ACHGMT_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6835726.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10006..10812
                     /locus_tag="ACHGMT_001992"
     CDS             10006..10812
                     /locus_tag="ACHGMT_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6835727.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10969..11937
                     /locus_tag="ACHGMT_001993"
     CDS             10969..11937
                     /locus_tag="ACHGMT_001993"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFN6835728.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12254..12778)
                     /locus_tag="ACHGMT_001994"
     CDS             complement(12254..12778)
                     /locus_tag="ACHGMT_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFN6835729.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13065..>14129
                     /gene="scm"
                     /locus_tag="ACHGMT_001995"
     CDS             13065..>14129
                     /gene="scm"
                     /locus_tag="ACHGMT_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFN6835730.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         3996 a         3046 c         2791 g         4296 t
ORIGIN      
        1 gttgtgacaa taatttgtca caacctcttt tttaaaatat ctattttgtt tgattgttca
       61 ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg
      121 ctacaccgac ttcttttggt tccatgtcac gataacgttg cataccagta ccagctggga
      181 tgatcttacc tataataaca ttttctttca aaccaagaag aggatctttc ttaccgcgga
      241 tcgcagcatc cgtcaagaca cgagttgttt cttggaatga tgcagcagat aagaaactat
      301 ttgtttctaa tgatgctttt gtgataccta gtaagacagg tcgagaagtt gcaggaacgc
      361 ctcctgagac tagcgtattg tagttttgat ctttaaagtc tgcgatgtct aataatgtac
      421 ctggtaagat atctgtatca cctggatcca ttacacgaac tttacgcagc atttgacgaa
      481 ccattacttc gatatgcttg tcgccgattt ctaccccttg catacggtaa acacgttgta
      541 cttcacgaag caagtagttt tcaacagata atacatcgcg aacttgcaac aattgttttg
      601 gatcgattga tccttctgtc aatggtgcac cacggtggat catatcgcct tctgctactt
      661 tcatacgtgc tgtataagga acagtatacg tacgtgtatc tgtttttcct ttgattgtta
      721 cttctttggt acgtgttgca ggatcttctg agatatcgat gacatcccct gttacttctg
      781 tgatcactgc ttgccctttc ggattacgtg cttcaaagat ttcttggaca cgaggaagac
      841 cttgagtgat atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag
      901 taccaggttc accgattgat tgagcggcga tcgtaccaac tgcttctccg acttcaacgt
      961 cagagccagt tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt tttgtattac
     1021 atgtgaagac agaacggatt gtcactgttt cgacacctgc atcaacgatt tgttttgcga
     1081 tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc atttgtttca ggatgacgta
     1141 cttctttacg ggtgtaacgt cccactaaac gatcttctaa tgattcgatg atttcatttc
     1201 cctcgcggat ggcttggatt tcaagacctc ggtcagtacc gcaatcttct tcacggatga
     1261 ttacatcttg ggccacgtca accagacgac gagtcaagta acctgaatcg gcagtcttca
     1321 aggctgtatc ggtcatccct ttacgagccc cgtgagttga gataaacatt tccaagacgg
     1381 atagtccttc acggaagttc gaaatgattg gcaattccat gattcgacca ttaggagcgg
     1441 ccatcaaacc acgcattccg gcaagctgtg taaagttgga aatgttacca cgagctccag
     1501 aatcagacat catgaagatt ggatttttcg cttccaaacc ttccatcagt ttttgttgga
     1561 tgctgtcttt tgcagcattc caaacagcaa tgacacgttc ataacgttcg tcatctgtga
     1621 tcagaccacg acggaattgt tttgtaatag tttcaacttg tttatgggca ttttcaatga
     1681 tttcttgttt ttcattcagt acactgatgt cagcgatccc tactgtgata ccggcatgtg
     1741 tcgaatgttt gtaccctaag tctttcatac ggtcaagcat cttagaagtt tctgttactt
     1801 tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt tttcttcttg aacggtaata
     1861 ctaattcttg ctctttgata tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt
     1921 ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt cagataaggg aattcaggag
     1981 gcatgatctc gttgaagatg atcttaccaa ctgttgtgat catcgtacgt tctttttgcc
     2041 attcagtaaa tggtttttca cctaatgagt tagggttcac accgatacgt gaatgtaaat
     2101 gaacatatcc gttgcgccat gcgataacgg cttcgttttg atctctgaag accattcctt
     2161 cgccttcgcg tccttcttct tccattgtca agtagtagtt acctagaacc atatcttggg
     2221 aaggagtaac aactggttta ccatctttag ggttcaagat gttttgagca gctaacatca
     2281 acatacgtgc ttctgcttgt gcttcttcat tcaatggaac gtggacagcc atttggtctc
     2341 catcgaaatc ggcattataa gcttcacata caagtggatg caaacggatt gcgcgtcctt
     2401 gtactaaaac tggttcgaat gcttggatac caagtcggtg aagcgtaggt gcccggttca
     2461 gcaatactgg gtgttccttg attacgtctt ctaaaacatc ccatacttcg tcttcttgac
     2521 gttcgatttt acgtttcgca tttttgatgt ttgaagctag ttcgcgttga acaagctcac
     2581 gcatcacgaa tggtttgaat aattcaatgg ccatttcttt tggaagtcca cattggtaca
     2641 tcttcaagaa aggtccaaca acgataacgg aacgacctga gtagtcgaca cgtttaccta
     2701 gcaagttttg acggaaacga ccttgtttcc ctttcaacat atgagaaaga gatttcaatg
     2761 ggcggttacc tggtccagta actggacgtc cgcgacgacc gttatcgatc aatgcatcta
     2821 ctgcttcttg cagcatacgt ttttcatttt gaacgatgat tccaggtgca ttcaagtcta
     2881 acaaacgttt caaacggtta ttacggttga ttacacgacg gtaaagatca ttcaagtcac
     2941 ttgttgcaaa acgtccacct tctaattgaa ccattgggcg taaatctggt ggaataacag
     3001 ggataacgtc cataaccatc cagcttggtt gatttcctga agcgcggaat gcttctaaaa
     3061 tatccaaacg gcggattgca cgtgtacgtt tttgtccttg tgcagatttc aattcttctt
     3121 tcaattcggc tgcttctgca tctagatcaa cattatcaag taattgttta atcgcttctg
     3181 cacccatagc agcttggaat tcttgtccat attgttcacg tttttcgcga tattcgcgtt
     3241 ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg ttcgatgact acatatgaag
     3301 caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg
     3361 aaggaatccc tttaaagtac cagatatgtg aaacaggagc tgctaattca atatgtccca
     3421 tgcgttcacg gcgaactttc gaacgagtaa cttccactcc gcatcgatca caaacgattc
     3481 ctttataacg gatacgtttg tattttccgc aggcacattc ccagtctttt gtaggaccaa
     3541 aaatccgctc acagaacaac ccttcgcgtt caggttttaa ggtacgatag ttaatcgtct
     3601 caggtttttt tacttcaccg taagaccagc ttctgatttt ttcgggagaa gccaaaccta
     3661 tttgcatact ttcgaattta tttacatcga tcaaaagggg ttccctccat ttcatttaat
     3721 ggcaaaccgc aagacggctg atcttcgacc gccttgcagc tcatagaccg gattagtcta
     3781 aattatcttc tgcggtttca aagttttgga tgacatcgtc tttttcatct tccacttgtt
     3841 cagcagcttt cttttctaat tctttggcag tttgctgttc ggcaaatttt gtcaaagcat
     3901 caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt
     3961 cgatatccaa gacacgcatg tccagaccta gtgattgcaa ttctttcacc aatacgcgga
     4021 atgattccgg tacacctggt tttggaattg gttcaccctt gacgattgct tcgtatgttt
     4081 tcacacgtcc gactacgtca tctgatttgt atgtcaagat ttcttgtaaa gtataagccg
     4141 caccgtaagc ttccagtgcc catacttcca tttcaccaaa acgttgtcca ccaaattgag
     4201 ctttacctcc caatggttgt tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca
     4261 atttgtcatc gaccatgtga gcaagtttga tcatgtacat cacaccgaca gacacacgtc
     4321 catcaaatgg ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg ctggccatac
     4381 cggcttcacg aactgtttcc catacgtctt catcacttgc accatcaaat actggcgttg
     4441 ctacgtggat acctaactga cgtgcagcca tacccaagtg taattccaat acttgcccga
     4501 tgttcatacg tgaaggcacc cctaaaggat tcagcatgat atcgattggt gttccatcag
     4561 gtaggaatgg catatcttct tccggcataa tacgggatac aaccccttta tttccgtgac
     4621 gaccggccat tttatcccct tcatggattt tacgtttttg aacgatataa acacgaacta
     4681 acatgtttac gcctggtgac aattcatctc cagcttcacg agtaaagatt ttcacatcgt
     4741 gaacgatacc gccgccgcca tgtggtacac gtaatgatgt atcgcggact tcacgagctt
     4801 tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga taattctgtt accccttttg
     4861 gcgtgacttt ccctacaagc aaatcgccat cttggacttc tgcaccaata cggatgatac
     4921 ccatttcgtc taaatctttt aatgcgtctt ccccgacatt agggatttcg cgtgtgattt
     4981 cttcaggtcc taattttgta tcgcgggctt ctgattcata ttcttcaata tggatagaag
     5041 tgtatacgtc atcttttacc aaacgacggc tcatgatgat cgcatcttca tagttgtacc
     5101 cttcccaagt catgaaacca actaaaacgt tttggcctag agccatttcg ccttgttcca
     5161 tagatggtcc gtctgccaga gtatcccctt tttcgacttt ctcacccaaa tgaacgattg
     5221 gacgttggtt gtagcttgtt ccggagtttg aacgacggaa ttttgttaca tcgtatttat
     5281 ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat tcaacgacac
     5341 cgtcatgttt acataacaaa gcagctcctg agtcatgcgc tgatttgtat tccataccag
     5401 taccaaccca tggtgcttga ggattgatca acggcactgc ttgacgctgc atgttggcac
     5461 ccatcaaggc acggttggag tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg
     5521 caactacttg tttaggtgat acgtccatgt agtctacttt atcaatagat acttctaagt
     5581 tttcactttg agcacgagcc ataacaacgt cttgagcaaa tgttccatct tcattcaatg
     5641 ggctgtttgc ttgggctacg atgtaatggt cttcgatatc agctgtcaag tagtcgattt
     5701 ggtcagtaac acgacctgtt tcacggtcta cacgacgata aggtgtttcg ataaatccga
     5761 atttatttac tttcgcataa ctggacaaac tattgatcaa cccgatgttt gggccttcag
     5821 gagtttcgat cggacacata cggccatagt gggagtagtg aacgtcacga acttcataac
     5881 cggcgcggtc acgagtcaat ccgccaggtc ctaaggctga aagacgacgt ttgtgtgtca
     5941 attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa ccaaagaatt
     6001 ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc aatgtttcag
     6061 tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc ccgatacgga
     6121 attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag tggtcgatat
     6181 catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa gcaatgatgt
     6241 cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc acgtttactt
     6301 cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt ggatctgtga
     6361 ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca atgaaaggag
     6421 ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg
     6481 tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta
     6541 attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga
     6601 aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac
     6661 gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt
     6721 ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat
     6781 cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt gtacggtcga
     6841 tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct ccacggttag
     6901 ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca tggaaataga
     6961 cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg atgatgaatg
     7021 ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc
     7081 cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag tttgcatcat
     7141 gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct acaaattcca
     7201 aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc
     7261 cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt ggtaattcca
     7321 acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat tttactacgt
     7381 gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc gatcaattgt
     7441 tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc ggcttttagg
     7501 caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc ttctactttt
     7561 gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc ttcatcagat
     7621 agtttttgca acttcatata ctacacttat tttctataaa agtcaacact tcctggcgtt
     7681 tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc aattatctga
     7741 caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc attttgtgga
     7801 ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa taacttttac
     7861 tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta aacgacagtt
     7921 tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag acaaaatgct
     7981 aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt tttcaattta
     8041 ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg tttcccaaaa
     8101 ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct gccctgctac
     8161 tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat ggacaaaaaa
     8221 caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat aaaggattca
     8281 aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc gattatcaaa
     8341 atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc
     8401 ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat gaaacaacca
     8461 ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta gtcacatcca
     8521 atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc gaaaaacaaa
     8581 tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg tataacaacg
     8641 gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa aaagcgggcg
     8701 tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc
     8761 tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca agtgcggcaa
     8821 gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc gcagttgatc
     8881 aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg ggtaaacaaa
     8941 cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa
     9001 cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta ggcatcaatc
     9061 cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa atttgctctc
     9121 tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga ttggtgtata
     9181 cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct ttcctttagg
     9241 tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag cttgtcttct
     9301 aggcttttta gggggtgcgg cagcagggct tgtttccgga ctgcttcaca caaaattgaa
     9361 aattcccgcc cttcttgcag ggatcatcac catgactggt ttatattctg tcacatcccg
     9421 tgtgatgggt gcgccgaaca tctctttatt gggtcagcca acgatctttc attgggtcga
     9481 gtcattcggc ataacaaaag acaacgctgt actgatcgtc ggtctgcttt ttgctttgat
     9541 cgttgtatgt ttacttgttt tgttcttacg tacagaaaca ggacttgcta ttcgtgcaac
     9601 tggcgataat ctggcaatga gcgaagcaaa cgggatcaac acagataata tgaagatcat
     9661 cggttatatg atttctaatg gatgcattgc tctttctggc tccttgcttg cgcaaaacaa
     9721 cggctttgct gatttaaatt ctggtattgg aacgatcgta attggacttg cttctattat
     9781 catcgcagaa gttttgttac gtaaccagcc actatttctc cgtctcttga ctgtcgtttt
     9841 aggtgcggtc atttatcgtt ttattttagc gttagtcttc gaactgaatg ttgaaccaag
     9901 tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt ctttctttcc cgcagatcca
     9961 gcaaaaattc catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa tgacgtctta
    10021 acattggaaa atctccatca tacctttgaa aaaggaacga tcaacgaaaa tcatgtcctg
    10081 aaaggcatta atctttcctt agatcccggc gacttcgtga cgatcatcgg tggaaatggg
    10141 gctggtaaat ctactttgct gaacagcatc gccggtacat tttctgtcga tcagggtcaa
    10201 attttattga atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc gaaatccatc
    10261 agtcgcgtat tccaagatcc taaactaggg acagccgttc gtttaacggt agaagaaaat
    10321 cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt caaaccacaa
    10381 gaccgaacat ttttcaaaga ccagctggtt cggctaaatt tggggttgga aaatcgttta
    10441 tcctcagaaa tcggtttgct gtctggagga caacgtcaag ctattacgtt attgatggcc
    10501 acattacagc gtccagaact gatcttatta gatgaacaca ctgcagcgct tgaccctgct
    10561 acttccaaaa cggtgatgga attgacagaa aagctgatca ctgagcagaa tctcactgct
    10621 tttatggtca ctcatgacat ggaagacgcg atccgctatg ggagtcgact gatcatgctg
    10681 catcaaggaa ggatcgtcgt agatatccag caagaggaaa aacagcagct cacagtgccc
    10741 caattattag cgttgttcaa acaaaacagc ggcacggcat taaaagacga tcaagtactt
    10801 ttatcttcat aatcagaaag aggatgggac aaaaaatgaa cgcccaccct cttctttttt
    10861 ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga aaactatttt
    10921 gttctctcct tcttcctgat agaatggatg ggaaaggagc ttgatgccat gacaacgaaa
    10981 gacaaggtac ttcaattatt gaaaaagaca ccagattttc tttccggaga aaagttggca
    11041 cagcaactgg aagtttcccg gacaagtatt tggaaagcca tcaaagaatt ggaaaaggca
    11101 ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc tgccctctga cgtactagat
    11161 gcaaaagaaa tccagcaaga gctacggtcg tttattcctg atttgaaagt acaaacgatg
    11221 ttgacttctg aatctacgat gaaagatgtc aaactggcag cagctcaagg agattctgtt
    11281 tcgaagttat ttgttgctga tacacaagaa caagctcgtg gacgttttgg acggcctttt
    11341 ttcgctgaac ctggacgagg aatctacatg agtctcttgc tccatccaga caaacggttt
    11401 gatgaattgc ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc cattgaatct
    11461 gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata tctacatgga tggaaaaaaa
    11521 gtctgtggga ttttatctga agcagtcagt gatatggaaa gcggcaggat cagtcatatc
    11581 atcattggga tcgggcttaa tttttctgtt cctcaaaatc attttccagc tgatctccaa
    11641 gaaaaagcca tctctgtctt tcccgacgga caagctagtg tcacaaggaa tcagctaatc
    11701 agcaaaatct ggcaagaatt gttcacttat ctttctcacc cagaaaaagc gttggacttc
    11761 tataaagaaa aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa aggaacgact
    11821 tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca aacaaatgaa
    11881 cagaagatga gtttgtcttc tggcgaaatc agtctcacat ctatccgtac gcaataatta
    11941 tcagatttca caaaaggaca cctgatctca tacagtgtcc ttttttctga attctcttca
    12001 aaagactctc cttattctat cagctgacag tttctctatc ctctagaaaa aggtattcta
    12061 aaaatatcta taaagcatgg tacactttct ttaaatgaat caatgcaaaa aacaagagag
    12121 tcatcagttt acacgatcaa aagagatagc aggccgcttc ctttttcaat cttatagata
    12181 gaccatgggt aacgataaaa acaaaaaaga acttgcgact tattttgccg taagttcttt
    12241 tttagctagt gccttaatct cttaatccca acaaacgtct cgcatagatc acctgaccag
    12301 agtgcatagc agcatcatca ataatagaaa cgagacgcat tcctcgtgtg actggtggtg
    12361 tccagttctc gtcaattact tcatctagct cggaaggttc cagccttctt aagtaagaaa
    12421 tagcaaaatc agcagctcct cgcaagtatc ctagcagatc ttctatttga ttagcttgga
    12481 tctgctttgc ttgttccaga ctatgcttcc aatcttgttc gttatctgct atcgtcatcg
    12541 gaaaccggct ttcccagtct tgactatgcc aaattggaga agttcctgct aaatcagcaa
    12601 tctggaaatc taattctcga gcggtatgcc aagccagcca agtcattgat ttgatctgcg
    12661 gggctctatc ttccactgga aaacgattgg cctcctcaat agagacgcct tcaaacaaac
    12721 gttcaaaccg atcgtatgcc cgatccacac tctcgcatag catatctaaa taatccattt
    12781 attttcctcc tttttattta tcctacttac tttttcctaa ttctaggata cctacttata
    12841 tgttactaca cacccttatt ttttacactt taacagtcac catcatcaat agtttggaga
    12901 ttgaaagaaa aagatagtgt cgaactatca attgaacaag aagcaacaat atgaacgttt
    12961 cattaatatt ctttaaataa acaatgattt ttcaaacgaa cagtttaaga ctaggagaaa
    13021 aagagtataa taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa aaaagggcaa
    13081 gtttactagt cctagttgct ttgttgttca atatcttttc gtatggtgtg gccgtttcag
    13141 cggaaacata cattcctaaa gaccctggga atatttataa tgatctaact ggtaactatg
    13201 gcttgttagg aattgctagt caattccacg tatttgctaa aaataagaca actgtgaatg
    13261 cacatacaga cggaaatata gcaacacatg aacttgatgc aaaaaataat tttggtacag
    13321 agcttcatga aggtttattg aaccaagaaa taaattatat acaaaaaact gattctttcg
    13381 ctaattcttc aggcatacct acaggtagca caagaatgaa taaatttgtc gttggcagta
    13441 acgtggatgt agcacttcaa gatggtcatc ctacagtcaa tggatccagg cttgatcact
    13501 taactccaga tgatttttat aaagatcgtg ctgggcatga atatattgat ttcgagcagg
    13561 aatttgctaa attgaatgta gcagcaaatg atcttgcaac gatcactcca gctaaaacat
    13621 atactgctgc agattttcca gacatgaaca atcggacaat cgatttaact gatttatcag
    13681 ataccgggtt tttattggta aatatcgatg cagaagtgtt aacgatgaat acgccacttc
    13741 aaatcattaa tccaaatgat caagtagttg tatttaatgt catcaacagt tcttctgcat
    13801 tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca gaagattttt
    13861 cggatgcgaa tatctcttgg aattttggaa atgagatgac tgatttgaca atcagtgctc
    13921 cttttcaagg aacaatcctt gcgcctaatg ctactatccg cattactcaa aaccaagacg
    13981 gaacgattat aggaacaaat gtgattttag atgcagctac caatcgttgg gatcctaacg
    14041 aaatatttat cagcgacaat ggtacagata cgacagacac aagcgacagt acagatacat
    14101 ctaccacaag tgatagctca gattcatca
//
LOCUS       DAZBSA010000072        13799 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000072 DAZBSA010000000
VERSION     DAZBSA010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.072"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            102..296
                     /locus_tag="ACHGMT_001996"
     CDS             102..296
                     /locus_tag="ACHGMT_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835731.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            565..864
                     /locus_tag="ACHGMT_001997"
     CDS             565..864
                     /locus_tag="ACHGMT_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835732.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(961..1395)
                     /locus_tag="ACHGMT_001998"
     CDS             complement(961..1395)
                     /locus_tag="ACHGMT_001998"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6835733.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1627..2337)
                     /locus_tag="ACHGMT_001999"
     CDS             complement(1627..2337)
                     /locus_tag="ACHGMT_001999"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFN6835734.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2327..3187)
                     /locus_tag="ACHGMT_002000"
     CDS             complement(2327..3187)
                     /locus_tag="ACHGMT_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFN6835735.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3192..3830)
                     /locus_tag="ACHGMT_002001"
     CDS             complement(3192..3830)
                     /locus_tag="ACHGMT_002001"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFN6835736.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3845..4144)
                     /locus_tag="ACHGMT_002002"
     CDS             complement(3845..4144)
                     /locus_tag="ACHGMT_002002"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN6835737.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4187..5671)
                     /locus_tag="ACHGMT_002003"
     CDS             complement(4187..5671)
                     /locus_tag="ACHGMT_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFN6835738.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5691..6152)
                     /locus_tag="ACHGMT_002004"
     CDS             complement(5691..6152)
                     /locus_tag="ACHGMT_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN6835739.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6170..7237)
                     /gene="ulaG"
                     /locus_tag="ACHGMT_002005"
     CDS             complement(6170..7237)
                     /gene="ulaG"
                     /locus_tag="ACHGMT_002005"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFN6835740.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7455..8225
                     /locus_tag="ACHGMT_002006"
     CDS             7455..8225
                     /locus_tag="ACHGMT_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFN6835741.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8498..9325)
                     /locus_tag="ACHGMT_002007"
     CDS             complement(8498..9325)
                     /locus_tag="ACHGMT_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6835742.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9893..11128
                     /locus_tag="ACHGMT_002008"
     CDS             9893..11128
                     /locus_tag="ACHGMT_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN6835743.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12127..12387)
                     /locus_tag="ACHGMT_002009"
     CDS             complement(12127..12387)
                     /locus_tag="ACHGMT_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFN6835744.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12414..13715)
                     /locus_tag="ACHGMT_002010"
     CDS             complement(12414..13715)
                     /locus_tag="ACHGMT_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN6835745.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4438 a         2826 c         2174 g         4361 t
ORIGIN      
        1 aattgattct ttttatttat cataaagata tagatacata atcattttca cttacatata
       61 tttgaatatt aactaaaaac agcaagaaag aagaggatca aatgtattat gtagaagtaa
      121 aaacaaaagg cgtcaaaaac aaacaatatg tgaaaggtat cagtaacgaa tatcctttac
      181 ttggttcatg gaaagaagct gctccttttt ccaaaccatg tgccataaaa ataaagaacg
      241 agctagaaaa agaactgact tgtggtaaag ctgttgtaga tatcatcgaa aagtaaatcc
      301 aatgtataga aaggaagatc acacatgaaa agcgacgtac aaattgctaa gattatagaa
      361 ttagaagaag aagacgcagt gttgacaatt gaaaaaaaat aaatgtttgt ataccatatg
      421 attatttcaa tatgtatcct ggaattggag atctcgtaga gctatatcaa gatgaatcaa
      481 atatacttgt cattcatcat gaaatataga gacttataaa attgtttatt tttataatgt
      541 atcagtatag aaaggaaata aaacatggat tatactgttg aatttgacag tgaactactt
      601 aagcaatttc cacaagaaga acatttcatg tataaagaag caatttccca caaagtgaca
      661 gagtatttgg agcataattt ttttcgtata aagccagtgc gtactcgttc ggcaaaagga
      721 atatacgaga tgaaagtcaa aattaataaa aaaaattatc gcatagcatt tgctttaaaa
      781 aaaaatcatg tgttcatttt ttatattagt tcgaatttgc aaaaaattgt atttgataaa
      841 gaggtgagta aaaaaataaa atagctgagc attggaggat agattgagtg atactaagtc
      901 tatcctcttg tttttctaaa gaactaagct agaatttttt cttttgtacg ttattatatg
      961 ttatttgtca ttaccaaaaa gcaccttttt tcttctagta tctatgacat aagaaatacc
     1021 acccatttcc tttacttgaa caaatcctac tgattcataa aatttttttg ttttctcagt
     1081 atcatctgtc aataattcga tttggactgg tccataacta gtgatcttct ccaaagcttc
     1141 attcaaaagc actttgccta ttccttggcg ctgatacctc ggatgaatga ggatatcttg
     1201 aataaatgcg atcgtcgcaa aatcagtgat ccaacgaacg attccgataa tcttcccttc
     1261 cccattacgc tttatcagag attcagattg ttcaaatgct ctagctaacc ttgcagtgtc
     1321 ttttgtgtaa ttcgtccaac caacgctctt atataattca gaaatctcat cgatcataag
     1381 ggaatcaata ttcatttttt tcttcctttc ttatacaatt ctccctctaa taatacagtt
     1441 ctttgactat ttaatctatc acatataact ctcctgtaaa gattctctcg attccttaaa
     1501 attcctcttt ccttttagaa tgcatacttt ttgataaata aaaaggttgt gataaatttt
     1561 cttcacaacc tctgtttctc tctatcaagt gttatctttt aattttctac ccttacccta
     1621 gcatctctat tgcccgtagt atgcatactt tccatgtttg cgaaaataat gtttatctag
     1681 taaatattgc gggatttcac atatattggg gttgatcagc tcgttttctt tggccatcag
     1741 acaaatttca tctaagatca aactgttttc tactgctttc ataggtgaat ccccccatgt
     1801 gaacggtcca tgtccataga caagtactcc agggacttcg tttggatcta attttctttc
     1861 cttaaacgtc tcgacaatca cattccctgt atgaacttcg taagcttctc ttacttcttc
     1921 tttcgttaat tggcgtgtac aaggaacctt accataaaaa gcatcagcat gagtcgtgcc
     1981 ataagctggc aaatcgcgtc ctgcttgcgc ccacatgacg gagtgcgtgg aatgagtatg
     2041 agtaatagca gttatatctt caaaagtttg atacaaaaca acatgtgtgg ggagatctga
     2101 agatggtttt aagctatctt cttctattat atttcccgac aaatctgtta ccaccatttg
     2161 atctgcttgc atgcattcat atctgacacc gcttggctta atgacgatga ctcctagctc
     2221 acggtccact tcactcacat tgccccaagt caatttgact aatccgtatc gcggcaatgc
     2281 cagatttgct tcaaggacac gttgcttcat tgctgcaatt ttttcttcac aagacatcat
     2341 agccagcctc cttcaattta gaacttaagt agtcttttgc ctgctggatt tctcttcgaa
     2401 agtcttggct tttttcactc cacatttcaa tcagaaatgt tccatcataa tctagacgtt
     2461 tcaaatgtct taataatcca agaaaatcaa cacaaccgct accaaaagga acatcacgaa
     2521 actggcctga atattctttt gttacagggt aagtatcttt taagtggata gcagtgatat
     2581 aatcgatccc tttctcaagt tctcgagccg gattattttc cggccatgcg gacaaattgc
     2641 ctaggtctgg atatacttgt agaaaaggtg aatgaatctg ttctttgatt tctaaaaatt
     2701 ttgaaatgga gttcataaat ggatcatcca tgatttccac ggaaagaatg atgccatatt
     2761 tagaagcttc ctttacgctt tcttttattc cttcgataaa gtcttctctg gaattcattg
     2821 atttctcttc ataataaaca tcgtatccag ctatttggat tactttgacg cttagattat
     2881 gcgccaaatg gattgctttt ttcattagtt gtttcgctgc ttttttcttt tctggatcag
     2941 cagaaccaaa cggaaatcgc cgatgcgcac ttaaacaaat cgaatggata cgtacaccgg
     3001 agctgaacat ctcttctctc agctcagcta tcttttcttc agaccaatct aatcgtgcta
     3061 accgctcatc cgtctcatca atcgacatct cgataaaatc gaagtccatt tcttttacaa
     3121 gggaaaaacg ttctttccat gaaatatctt tgggtaacgc tttttcataa atcccaattt
     3181 gagccatttc cttaccccca gatacgaatg atctcatctt tgaagttttg tgcggccttc
     3241 atcggatcat ttgctgctgt aataccacga ccagtgataa aggtatacac atcgatccct
     3301 tcaaataagt gtaatgtgtg tggatttaat ccaccagtta cagaaacatt aaagcctagt
     3361 tcaatcagtt ttttgatctt cgataaatct ttttcacccc aagtttcacc agataacagc
     3421 gcatcacggc tttgatggta gattacctgc cggataccga tgttatgcca atccatggct
     3481 tgttcaaaag tccaattgcc gtagagttct acttgcaatt cacctatttc tttttgtgct
     3541 gcctccatag ttggaatcgt tgcacaacaa atgactgtca tccagtctgc tcctgctttt
     3601 gcaacattac tcgccactgt tcctccagcg tctgcacatt tcgtatcagc gactagtttt
     3661 ttatctggga acatactgcg aatacagcga atcgcttttt gtccttcctg taagcaaaga
     3721 attgtgccca cttcaataat atctactaca tcaccaactt tcataacatc tcctaatgca
     3781 tgttctaagt cattgtgatc taacgcaatt tgcaaattcg gcctgctcat cttagttcct
     3841 tcctttactt gtttttttgg taccactttg tttcagcaaa tttttcactg atttctttga
     3901 ctgacatcac atttttgata ccgataactg taactccttt tttctctgcc tgagtaaaca
     3961 tattcaaaaa attgactggc gtaaaaacta catcgtattg gccagctgaa ctttttccct
     4021 ctgaaatggc acaatgatgg attttggtaa ttggtacgtt tagttctttc attgcttttt
     4081 ccacacttct catcatcatc aagcttgttc ctgatccatt tgcacacgat actaaaattc
     4141 tcatcttaat ccctccatta tgagtgttgt ctaatcagat tttcttttat actctctgct
     4201 tttctgcata tgtgtcatag tcttctacaa tcaaaaaata accatcagga tttttccgat
     4261 attgtaactg cggaatagcc aataaaccaa tgacaactac ggcaattcca gcataaccta
     4321 ggactttcat gattactgta aacaatggcc aagctgttgc ccagtcgaac atcccgatat
     4381 aaccaccgta tgttgacatc ccaatccatg tagcgaataa tgctgaaccg cctacttgta
     4441 tgattcctga aagaaacggg aacaggcaag cggctttgaa tccacctcgg ttattggcat
     4501 aaactgctat aacggcatta tcaaagaaca acggaatgaa accggctatg acgatcgttg
     4561 gtgatttcaa taaaatcagt aaaccaatca tcaaaaactg tcccaaagca ccaaacaaga
     4621 acccaatcgt taccgcattt ggcgaaccaa agccaaaagt agcagctacg tcaattcctg
     4681 gaacagctcc tggtaaaatc gtgtttgaga ttccttggaa agattgagtt agttcatcga
     4741 cgaatgtccg aacacctaat tgcaagatcg ccagatatac cgcaaaattt aaagaagtcg
     4801 tcataatgta gaataagaaa ctctgccctt cttgcatgaa ttgcgcttga atcaaataat
     4861 ctttgcctag tacaatcaga atgatcccaa agaaaaatag catgagtaac gaagtagcaa
     4921 ccatattctc attgaaaatc gacaagaatc ctggtaattt gatatcttca atttttttgt
     4981 cttgcttgat tgatctattt ttcttttcat cccgcttttt catccactca gctaattttg
     5041 caaatataaa gattccgaac atttgctgat gtgccactgc aaatccggca ccttctgtca
     5101 attcctgcgt gattcctact gttaagttag aagctactgt ccaataacaa cctaaaatca
     5161 gtcccataaa aatcaatgct tgtatcctac ctagatcagg aaagcaaaac agtaaaatcc
     5221 aaaaagcggt agctgcttgt tgaacctgaa cgtttccggt agtgaaaacg gaacgcagtt
     5281 tcgtatattt ctgaaaacgg actaataaga tatttaaaac aaatgcaatc agtaataaaa
     5341 tcatcacatc tccaaaggta cggccaaatg tctccattaa tccagcatcc acagcatttt
     5401 gtccaaaata aggatctgta accatggcat ccaaattgaa ccgttccttt agtccaacca
     5461 agattggccg aaaattattg accaaaccac cagagccaac agtcaaaata aagtaaccga
     5521 ccgttgcctt taagaatcca gcgatgcatt catgaagcgg gcgtttcagc aaaatatagc
     5581 ctaataacac gataaaacca atcaggtatg ctggctgcgt caagatattt gttgcaaaat
     5641 acgtccaaat gcttaacaaa aattccccca tcttttttct cctctatcaa ttaattatat
     5701 tctgccatca caacttggta atcatcgatg gagttcgtag agatgagacg ctcaaccaat
     5761 ccatctgtca ttaataattc ggataatttt tggatatttt ctagatgttc ttctggatct
     5821 tttgctgcga gtgtaaaaaa caaactagcc gttttctcct cattctcccc ttcactttca
     5881 aaacggattg gctgtttcaa cttggtaaat gaaatagctg ttccaaaaac accatcactt
     5941 ttttctgaag aatgcggcat tgctacttga ggtgcgatca caatataagg accgtatttt
     6001 tctacacaat ggatgatctc ctccacatac gtttcatcaa taatttcctt atcgatcaat
     6061 acaagacagc tttcggcgat agcttcacgc caatccgtga tttctttatc tgggtaatga
     6121 atcaattgat ggtcaaaaaa gtatttcagc aagaaactcc ccttctttct tataaaaacg
     6181 acggaaacgg tgtatcattt tcaatcgaga agacatcttc aaatcctcga taaaaattaa
     6241 actcaagatc atctttgttt gtcggataga tgaatttccc gcctacttgc cagatataag
     6301 gtttaaatcc atacttcagg cgatcctttt tgaatttcca aatttcagtg atctcttttg
     6361 gatcagccat aaaattagac caaatatcgt agtggacagg aatcactact tttgcattca
     6421 atgactccgc catgcgcagc atatcaacag aagtaacttt gtctgttatc cctcgtgggt
     6481 tttcaccata ggcccctaaa cagacatcga ttttgtgttc attaccatgt ttggcaaata
     6541 aattggagta atgtgaatct cctgcatgat aaagattccc tcctgatgtt tcgaataaat
     6601 agtttactgc aatttgatcc atctcctgag gcatttttcc ttttaatacc tcttctggat
     6661 cttcacaagt gaccaacgct gtccgatcaa acgcttctaa agctactaca ttgatgtctt
     6721 tgattttgac tgcatcacct ggctttactg taatcgtttt ttcttcagga attccccatt
     6781 tttgccacgt gcgaaccact tcttttggcc caatgaatgt tgcttcagga caattttgat
     6841 gaactgctgc ggctgtatgg atatctagat gatcggaatg gatatgtgta acgattaaag
     6901 cgtctacatt ttttatcgca aaaggatcga ttacgaaagg ctgagtacgt aaattaggct
     6961 gcatattttg aacgccactc atacgcatca tctgatggcc ttttttcatc atgccattcc
     7021 catgactacg ttttcccgtc ccacaccata gatcacataa aatattcgtt ccttcatgac
     7081 ttttcagcca aattcccgta cagcccagcc accacatagc aaaagttcca ggatccacta
     7141 cttctgtttc gatttcttca ttcaagtatg tgccccactc aggaaatgtt tctactaacc
     7201 acttttcttt tgttacttca ttgacagttt tactcatttg cttgctcccc ccatatcgtt
     7261 gttccaactt acaagtaaat attaatgtgt atcagcatcc atgaaaaccc ttttatatga
     7321 ccgtttgttt tttatatgat tttctttcag taatcaatga ttgtttcagt ataataaaga
     7381 cagatatgaa tgtttaaatg aacaactagt caaataaatc catggatcaa gatgtccctc
     7441 aggaggcaaa agtcatgaaa aactcacttg tcaatattga aaaacgtcaa aaagaaattt
     7501 tggaactgct tcagcagact gaacaactat caactttcga attagctgaa tcccttgatg
     7561 tatctattag tacgatacga agagatttaa atttattaga agaaaaaaac gagatcatac
     7621 gcaattacgg ttattgttct tataattaca aaaaccagac tgattttgat caatctggtc
     7681 ctgaaaggat taaacaagcg attgccagag aagctagtaa atacattaac gattatgata
     7741 cgctgtttat caattctagc tcgactgcgt tgaaaacact ggattttctt caatctaacc
     7801 atttgacagt cattacaaac aacgtgaaaa tcaattactc tccgcacaaa gataattgta
     7861 gttatatttt aactggtgga gagatgcgct ttccaaaaga agcactagtg ggcgacatcg
     7921 cattaaatac gatttcttca gtcaatgcag acatttgtat cattggctgt agcggagtcg
     7981 atgtagtaca tggggtcaca acaaagatat taaatgaatc aacgatcaac gaaatgatgg
     8041 tcaacaagac aatcaaatgt aagattttag tcgctgatca tcgaaaaatc ggactgacat
     8101 caaagttcaa gattgctgat atttcagtct ttgattattt gatcactgat aaattttgtt
     8161 ctgaaaaagt attagaggag atcagaaata cggggataaa aaccattcaa gtagattcat
     8221 tttaagaacc aggacgtaaa tatgtaaata ggattatcaa aaaaatgaat aacaaattta
     8281 tcgataaatg aaaaacacgc tacgctcaaa ttgataaagg aatcccaaga tgtatccatc
     8341 caatcgatac catcataatc tattgcttct tttataaccg ttatatgttg cctaaatagg
     8401 aatcaaacaa tatcgaaaaa aggaggtatg acaaaagtca cacctccttt tttcgaataa
     8461 acggtattca aatcagaaca ctgatttcac aaccacttta tttcaggtcg ttttcaatca
     8521 cctttaccgc gtaatcattt ccgttgaaac gcgctatttt ttttgcgtcc atatatcttt
     8581 ctaaataatc atcttctcct aattgcttat ggcaagaagc aatttcataa agcaagtatt
     8641 ccaatgcata agtcgattta tgtttcttat tatactcaat acccttctca cataattgga
     8701 ttgctttatc ataatcctcg attttacggt aataggtggc gatatcataa tatacaatgc
     8761 agaccatcag attatcaaaa tcagtctcac tcaatatttt aagtgtacgc tccacataaa
     8821 ctttcgcaaa atctaattct ccttttgaca agtagatcat tgccacgttt gcagctgctt
     8881 gtaagtgatg gatatctgtt tcggtcgttt gttccaacac ttgattaaaa taaaataatg
     8941 ctgcatctgg ttttttcgca taaagatgat caagcagtcc ttgataaaat aaaatttgat
     9001 ttaaaagatc agaattactt actttttcct gatcgatcgt ttgtaaaatt ttataagatt
     9061 cttggtattt tgatacagaa ttcaatgatt gaatctcatt tatttttttc ctgattttgt
     9121 cttcttctgt ttctactaat atatcggaga tctcgacatt cagctttttg caaagcgcac
     9181 ttaagatatt gatgcctggc gacatattct tgttttcgat attgctgaca gttgcttgtt
     9241 cacaaatgcc ttctgctaat tgtacttgtg ttaacccctt tttctttcgg tattctttta
     9301 gtaatgctcc atctacttta atcaaaacaa tttccttccc tttaaaaaat tatttctcct
     9361 ttatatcata cacaaaaaaa agttctaaac aaccaaaaat gtttagaaca gaataaaaat
     9421 tttaatttta aaataatatt caaattaaac aactggcgta tctgctcctt gatttctttt
     9481 tgtgttatct gaataattat cgtttacagt tataacacat agtgaaaatt gtcatggcga
     9541 gaaagtctaa gaatattttt aaacataact atttctttct tacgtgtgta taatgacgac
     9601 agcacaccta tcaattaata taattatttt atttttttat gttctcatgt ttatttttaa
     9661 tgacacaact tctcgtattt atctggcaca acatataaat cgatcaaaaa attgttgcgt
     9721 gtttttttga aaattggagt ttcaacttga ttacaaaatt taaatttaga agttgtgaaa
     9781 acagcgagag acagtttaat attcaaaata tacataggct actcgaacgc tgtgtttttt
     9841 agtatactag taagaaacga agggatagac tcattccaat aaggaggatt tcatgaagac
     9901 aattcttttt gctcctgcag tattcaattt agctgagaca acacgaatga ttgaggttgc
     9961 caaagctatg aaagaccgtt attactgtgt attctttggc ttttctaaaa cttttgctcc
    10021 tttgatcaca gaaagtggct ttgagtatca tttactagcg ccgatcctta cgaaaaaaca
    10081 agaagaacaa atcataaaat ttgaccagtt gcgtacactt aaaaaccctt ttaccaaaga
    10141 aatggttgcc tctcgtatca ctagtgaaca gagtctgatt gctaagtttg agcctcgact
    10201 gattgtgaca ggttcgaatg tgacaatttt tttatcggcc cgcaataaaa agattccttt
    10261 agtatatgtt aaaccctatg ctttgagtga accttttttt caatcagagc atgcgctgat
    10321 gccccacgag ttacgtttct tcggcccttt aagcaatccc ctttggcact ggacaaaaaa
    10381 gcgtatagta aatacccgat ggatccccaa tgccttcaaa cacgctgcac aaaaagaaat
    10441 gcttccttta ccaaaaacaa gtttacaaat gatggatgct gatctaaatc tcatcacaac
    10501 gcctaaacta tttttggaag aagaacagct gccggaaaat tatcgaatcg ttggtccaat
    10561 ttttgctaag ctcagtactt ccttgcctga agaagtgcta acttttatac agcgaaagca
    10621 acaagaaaga aaacgtatta tctatttcgc aatgggtagt tcaggcaatc cgcgtatgtt
    10681 acgcaaaatt ttgttcttct tacaaaaaca accggatcta gcggttattg cacctattga
    10741 ctatctgctt gatgacattc aatctgactc agaagatctt ttaataacag aatatctgtc
    10801 tagtcatctt ttgcatgaat tcattgattt ttcttttatc cacggcgggg aagggaccgt
    10861 ccaaactgcc tgtgaatccg gcaaaccgtt tatcggtgtg gggatgcaat ttgagcaaag
    10921 ctgtaatatt gactattgtg taaaatacgg aaacgcaatc gccctcacaa aaccagtgac
    10981 tgaaagaaaa ctgcagattg ctctgcgcga agtttcttca cccaagattc gtcagcaggc
    11041 acgcacgctg caagccgttt ttccaataaa tggtccacaa caagcaattc aagaaatcga
    11101 aaccttcctg agcaaccacc caatatagtt tcaagtagaa aaagcttttt taatagagac
    11161 tcatcagtaa tttcgctatt cgagacccag acagtgaaaa tacaaaaaaa gtaaatagtg
    11221 gattgaaaag ttagagttac ttaaattacg gtgtctaaaa aacacaaaat catccttaaa
    11281 ataaaagatc tgacgctatc caaatgagca taggtagcgt ctgccattta ttcttagtca
    11341 aattctttga cacctttctc tggttggatc atctcaacgt ttcttcaaaa gaaagaccat
    11401 aataattagg caaatacatt ttcttaaaat gtgtcatata gatctagaag catcgctttg
    11461 tagagatttg tgccgctagt cccctcaaca attactaaat caaatgtagg atttaccact
    11521 ttatctttga taatcaacct ccctcttctg ttgagacaat gatctcgcaa tccgatgcat
    11581 actcctatat aaactgccag caatccccac atattgagta gatcatgtca gaatgttcta
    11641 tgtcgtcagc aacgatacac taaaagtcac gttcaccatg actgattgct attcccagca
    11701 caattatttc tatacacgtt tcatgatttg aataaacact cacatcatac atattaccat
    11761 tcatacacca tactgcagcc cctactatat ggttgtaagc tcccaaatct atatttgact
    11821 tgatcacttt ggtagtagat ctactaattc taaatccatt gctgataaaa tttccatttt
    11881 ttcctccttc tttctgccaa aacagttaaa acaataataa tctttatgta aaaaagggct
    11941 gtgacaaaag ccttgtcaca gtcccatatt ccgaataaac ggtggtcaaa agcttgcatc
    12001 tgcggtaata agttcaacca aacgaaaaat aagccaacat ttcacaaaaa tttaagaagc
    12061 aattttcgta aatttcttct tatttcttga agctaaccac ttctgttaca accactttta
    12121 gaacatttaa agcttcgatg tgacaaacca tctatgctgc tcgatcataa aattgatagc
    12181 aaaaattcca caaagtacat atgtccccca tgaaaacaaa acattcatat gcggtacaat
    12241 gatctttgga agaatcacga tcaaaaatga tgccagtgcc caaatccatc gattgatttg
    12301 aatatgatat gtagctagca tattcaatat aaggtgacta gccgttaata aaaagataaa
    12361 aacaaaaaat aaaatcagcc aatacattgt ttcccacctt ctaaaaacca aacttagaca
    12421 gtctggactt cgttttctcc acgactttgt tcatctgaca gcatcttttt atcataagct
    12481 tttacaaatg gataatagat aataaatgct atcaatgtgt tgatcatgat caagataaca
    12541 acacgccagt catttcctgc tgaaagataa ccgccgattg gagctggtaa tgtccatgtt
    12601 ggcaaaacag taaatctatt gaccatttct aatttgactg caaaataact gataatagtc
    12661 aaaactaaag gaactaagtt gaacgggatg aacatgtaag gattcaacat cataggagct
    12721 ccaaaaatta tgggctcatt gatattgaag ataccaggaa taatagaaaa acgaccaacc
    12781 tctttcaaga attttgattt acagaagaat ataaacaaaa cagataccac gatcgttgct
    12841 cctgcaccac caattgttac cgtccattga taaaactgtt caggagcgat atagggtaat
    12901 tgggtggcag ctgtaccatc tgccaatgcc tttgcattct catccaacat gatcagccac
    12961 attggacgaa caacagcccc tacaatactc atgccatgga taccaaatga ccacaacaaa
    13021 tggatcaaaa acattggtag taaaacacca agcaaattat tccctaaaat attggttaaa
    13081 ggctcgaata acgataataa ggcagcgttt acatcaaagc ccgcgacgct tcgaatcagc
    13141 caagtaattg ttaaaatgac tgcacctgga atcaatgcct caaaactacg ggcaacagca
    13201 ggtggcactt gttctggcat tttgatagta atatttcgat ttttaaagaa acgatatact
    13261 tccactgcaa aaatcataga taagatcccc gcaaacatcc cagatgctcc caaagaactc
    13321 ataggtaaga cccagccaag aggtgcctct tcaggagtta aaacgtttac aggaacttgt
    13381 aacataaaga acgttcctaa agataatata ccacctgtca cactatctaa tccatatgat
    13441 ttagctagat ccgaacccat aacaaaagtt gcataaatag ccatcaatcc catcgtcatc
    13501 ctaaagggta aaacaatcgt tgccgaataa ggggcaataa attcattcca tccaggaaac
    13561 ggtaaattga taaagaccat aaaaaatgtc ccaatcaaaa tcaatgacaa tgttgaaaca
    13621 accccattac gaattgctaa taaatgacgc tgtgtaccaa tttttgccat tggtgggaca
    13681 agtttctctt cgacccattt catcagcttt tccactaaaa ccactccttt tcaataaaat
    13741 atgaaccatt cattgcatga caacgcaaag aatgttcata actaattata taattaaga
//
LOCUS       DAZBSA010000073        13738 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000073 DAZBSA010000000
VERSION     DAZBSA010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.073"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            13..2178
                     /gene="acm"
                     /locus_tag="ACHGMT_002011"
     CDS             13..2178
                     /gene="acm"
                     /locus_tag="ACHGMT_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFN6835746.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2450..2935)
                     /locus_tag="ACHGMT_002012"
     CDS             complement(2450..2935)
                     /locus_tag="ACHGMT_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFN6835747.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3025..3370
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3415..4056
                     /locus_tag="ACHGMT_002013"
     CDS             3415..4056
                     /locus_tag="ACHGMT_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFN6835748.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4075..4710
                     /locus_tag="ACHGMT_002014"
     CDS             4075..4710
                     /locus_tag="ACHGMT_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFN6835749.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4728..5543
                     /locus_tag="ACHGMT_002015"
     CDS             4728..5543
                     /locus_tag="ACHGMT_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN6835750.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5862..7298
                     /locus_tag="ACHGMT_002016"
     CDS             5862..7298
                     /locus_tag="ACHGMT_002016"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN6835751.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKSSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7487..9919
                     /locus_tag="ACHGMT_002017"
     CDS             7487..9919
                     /locus_tag="ACHGMT_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFN6835752.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQLSFVPI
                     DSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELPT
                     VDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAVG
                     VKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPNH
                     PDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEV
                     KLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNI
                     SGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGI
                     HMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLP
                     MGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELF
                     EEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQE
                     ETSETLPDDNDM"
     gene            10149..11216
                     /gene="rlmN"
                     /locus_tag="ACHGMT_002018"
     CDS             10149..11216
                     /gene="rlmN"
                     /locus_tag="ACHGMT_002018"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFN6835753.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            11206..11412
                     /locus_tag="ACHGMT_002019"
     CDS             11206..11412
                     /locus_tag="ACHGMT_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835754.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(11409..12512)
                     /locus_tag="ACHGMT_002020"
     CDS             complement(11409..12512)
                     /locus_tag="ACHGMT_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFN6835755.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13064..13612
                     /gene="aac(6')"
                     /locus_tag="ACHGMT_002021"
     CDS             13064..13612
                     /gene="aac(6')"
                     /locus_tag="ACHGMT_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFN6835756.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         4702 a         2296 c         2906 g         3834 t
ORIGIN      
        1 gagatagaaa aaatgaaaaa gtgtagaaat attattttat caatgttatt tattattact
       61 aatataacat cactgattcc tgttcatgtc tatgcggatg caggcagaga tatcagcagt
      121 aatgtcactt cgttgaccgt ggacccaacg aatattacag atggaggaaa cataaaagta
      181 aaattctcgt ttgacgaaaa aaaacaaaat atccaaccag gtgattatct ttggatcaat
      241 tggccaagtg aaggaaatat tcgcggtgaa ggattccaaa aagaaatccc attaatgatt
      301 gaaaataaaa atgttggaac tttaacggtc agaaaagatt ctgcgcaggt tgtgtttaat
      361 gaaaatatta aaaacctaga ctcagtagaa ggttgggggc aatttgagat acaggccaga
      421 aacgtaaccg ataccggtga agaaaatacg ggaccattca ccgtgacgag cggtgataaa
      481 acagctactg taaatgtgac aaagccagct tctggttctt cttctagcgt tttttattat
      541 aaaacaggag acatgctacc tgaagatact aagcacattc gatggttttt gaatattaac
      601 aataacggca cttatgttga acaaccagtt aaaatcagtg atgaaatcca aagtggacaa
      661 agattagatc caagtacgtt tgaaattaat caaatacact taggtgaaca gaaggtttat
      721 agaggagaag agggaatcca acaattttta caagatttcc ccagcgctac atttaatttt
      781 tcagtaactg ataattacat tgaaataact ataccaaaaa actttgtcaa tcttaggaaa
      841 ataatggtga gttataagac gatcatagaa aacccagaac aaataaattt tgagaatcat
      901 tcggaagctt ggtttaaaga atttaataaa cctgctgttg atggagaaag tttcaatcat
      961 acagttaaaa atatttcagc ttctggtgga gtcaacggta cagttagagg cgaattgaag
     1021 attttcaaat atatcaatga cacagagatt ggtatcccaa atgtgacttt cgaattaaga
     1081 cgagcagatg aacagcctat tcaaggacag tctagtatcc tgttgacttc gaatgaacag
     1141 ggagaagcta gtatcaaagg cctgcaagta ggagactatg ttgtaaaaga aaaagaagca
     1201 cccaattgga ttgattttga tccactaagt tcaaatgaac tcaaattctc cataaacgaa
     1261 aatgatacag agggagtcag tttacctatt tataacaaga aaaaggttac gaacatcaca
     1321 gcaactaaaa tttggaacgg tggaaccact ccaagaccga gtatttattt taaattattc
     1381 agagcaagta caaacaattg ggaaccagtt cctgatgcag aaacaaaacg attagacaat
     1441 ggaataactt cagtgacttg ggaagatatc caacagtatg atgatagtgg aaacgaatat
     1501 acgtttaaag tgcaagaagt agatcaaaac ggaaatgatt atgtcccttc aggttatcaa
     1561 aaaattgaaa acgggctagt cgttacaaat gagaataaag aagtcatttc agtctcagga
     1621 caaaaaacat gggaagacaa tgagaaccaa gacggcaaac gaccgacgac gatcacagta
     1681 aatctattag ccgacggtca accgatccaa cacaaagaag taagtgaaaa agatgattgg
     1741 cgctatcgtt tcacaaatct gccaaaatac agagacgggc aagaaatcat ctatactgta
     1801 acggaagaca acgtgcccga atacagtacg acgattgacg ggtataacat caaaaacagt
     1861 tatacaccgg aaaaaacaaa tatatctata ccaggtagcc atataacacc gtggaaaaat
     1921 tttccagaga agacagagag agagaaaatt agaaatctat tctctcaaaa attgcaaata
     1981 tttgctcata caggagaatc cagcgaacaa agaagcaatt atattagtaa gagagctgta
     2041 cctaaacaga taacaaataa atcaaaaagc attcttccta aaacaagtag taaaaaatct
     2101 agttttgctg taatcattgg tttattgata gtaactatct gtgctgggat attcttacaa
     2161 aaatataaaa aagtttagtt agaatataat attttttgtt aaatatgact gaaatcaaaa
     2221 gaataaaatt ccgatttata atcgaaaaaa gaagcatttt tatatatgct tcttttttcg
     2281 tagaatcaat aaatatggat ttggctaact atggaagaat actcgtatta gttttcagcg
     2341 ttcatactag ttgttacatt gtttgattgg ccaaaagaat ttctgatata ttttgtgcta
     2401 aataaagaaa aagacgcttg aattgggaaa taagaaaact actctatcat tacaaatttt
     2461 cataaactgg cagatgttct aagtaaacaa gtatcttttc aaagtcggaa aaatccataa
     2521 tcaacgtagt ggtattgata tttggatgaa agcctacggt atcttgacga tcaatagcag
     2581 catcgatcac gacttggatt tttttctctt tgtcgtgcat caatgctaat ggagtcactg
     2641 tacctgctgg aacgcctagg aggtcaggaa gctgttcttc agaaacaaaa gataagcgct
     2701 tttcttttaa ttgttcagct agttgtttta aatcggcgtg cttttcatca ggcaagatga
     2761 caagatagat ttgttttcct tttcttgatt tcaataatag attttttact tgcggaccag
     2821 gtagggaaaa agggacattt ttgactgacg taatcgcagg atgatcgact ttttgaaaag
     2881 agatacccag tttttttagt agttcataag cttcttgttc ggttgcacga ctcatcgcgc
     2941 atcaccctcc tttttttatt agtgtatcat aaaaaagagt tgacaaatga aaaataatca
     3001 tgtaaattac acgtaaagtt aaaacaaatg acatcgaaaa gaagagtagc cgttaggtgt
     3061 ttttagagag tctctggttg gtggaaagag gcagcagtta accgtgaaac acatcttgga
     3121 gtcgattttc tgaagcgtat agtaggaaaa tccggggagc ccgttacagc tgcggtcgtt
     3181 gctagatgac cagataaggc tggcttttcc gtgaggagtc ggtaaaaaaa ggtggaacca
     3241 cgtgattaac gtcctttgat cttcggatca aaggactttt tgttttcatc tgaacggcaa
     3301 cgactgtgtg aggttttttc tgcaaggaaa aaataaacag aggtggaacc acgtataata
     3361 cgtcctcttg gcatttgcca agaggatttt tttattttaa aggaggaaaa gaagatggag
     3421 tatttgattg aagttatgcc gcaattatta gtaggagcac tcacaacatt gaaattattt
     3481 ttatttacct tgattggctc attaccatta gggattgttt tagcattagg attgtctttg
     3541 cattttaagc cgctacgcta cttgcttcaa acatatgtat gggttatgcg gggaacaccg
     3601 ctgttgttgc aactgatttt tattttttat gggttgccga cgattgggat cgtttttgat
     3661 cgtttcgaag cagcactcat tgcctttatc ttaaactatg cagcttattt tgcagagatc
     3721 tttcgtggag ggatacaatc gattcctcaa ggtcaatatg aagcagcgca agttttgcaa
     3781 ttaagtccct ttcaaacggt gacgaaaatc gtcatcccac aagtgacaaa gattgtttta
     3841 ccttctgtag ggaatgaagt tatcaacttg gtcaaagatt cttccttgat ctatatatta
     3901 ggtttaggtg acgtaatgcg tgctgggaaa atcgcgatgg aacgcgacgc gactttactg
     3961 cctttgctag gcgtggcaat catctatctt acattgactg gtattctaac aatcgtaatg
     4021 aaacaagttg aaggaaaatt aaattattac cgctaactga taggaggaag aaatatgatt
     4081 gaattgaaga aagtgaacaa agcatttggt gaaaaaaaaa taatcaaaga tttgaatcta
     4141 atcattccag atgggaaaac attagctatc gtaggtcctt ccggtggcgg taaaaccacg
     4201 cttttacgta tcttggctgg cttagaaagt tctgacagcg gagaatttta tttagacggt
     4261 gtaccgttca acccattcga gcttaaaatg aaagaacaag ttgtaggagt cgtattccaa
     4321 gacttccagc tatttccaca tctgaatatt tttgaaaaca ttaccttagc accaaaaatg
     4381 gtattgaaac agacaaaaga agaatacacc aaaaaagcag aagaattagc ggcttcatta
     4441 gggattagtg atttattgaa tatctatcct tatcagcttt ctggcggaca aaaacagcgg
     4501 gtagcgattg ccagagcatt ggctatgaat ccacgggtat tggcttatga tgaaccaaca
     4561 agtgctttag atcctgaatt acgccagcaa gtcgagcgtt tgatcctatc attgaaagaa
     4621 gatggggtaa cccaaattat tgtcacccat gatttagtat ttgcagaaaa tgtcggtgat
     4681 caattactaa aagtttcccc aattcattaa gaaaggagaa gatagagatg aaaaaaacca
     4741 gttttctatt cgctttgatc gcaggtcttc ttctttttgc aggctgttcg aacaaaaaaa
     4801 cttcagcaga taattgggcg actatcaacg aagagaaaaa aatcgttata ggtttagacg
     4861 atacatttgt tcctatggga tttagagata aagacgggaa gctcacagga tttgatattg
     4921 atttagccaa agcagtcttt gaacaatatg ggattacggt agactttcag cccattgact
     4981 ggtcaatgaa ggaatttgaa ctgaacaatg gaacgatcga tttgatatgg aacggctatt
     5041 ccaaaacgcc ggcaagagaa aagaaagtgc aatttacaaa accttatatg gaaaacgatc
     5101 aagtgctgat cacacctaaa gaatcaggaa tcacaaactt tggacaaatg aataataaga
     5161 gattaggtgc gcaaaatggg tcatctggtt atgatgtatt tacgaaacaa ccagaagtat
     5221 taaaagagat agtcaaaaac caagatgctg ttttgtatga tagttttaat gaagcattga
     5281 tcgatctgaa aagcagacgg gtcgatggtc tgctgatgga taaagtatac gcagactatt
     5341 atttgaaaca aagaaacgaa ctagaagact tcaacataac aaaaggttct tatgagagtg
     5401 aggattttgc agttggtgca cgcaagtcag atagtacatt agtgaaaaaa atcaatgaag
     5461 ccctagatca actagagaaa acaggtgaat tccaaaaaat atccgataaa tggtttggcg
     5521 aagatgttac gccgagaaat taacaaatag gggactgtga caaaagacgt gtcacagtcc
     5581 ctttttccga ataaacagtg ttctcctgcc aactccttgt gactcggagc tgaatggcag
     5641 gctcacaacc tcttggtaaa cggtgttcaa aagccagttt ttcggtatta agccaaattt
     5701 atgcaaaaat gtagaaagcc attttcacaa aatttttctt actactcgaa actaagcgac
     5761 tttttcacaa cctcctttac ataattttat tttgttgtgg aaggggtttc gtgttattct
     5821 aaaagaggaa cgattgaagg ttataggaaa ggaagagatg aatggaacac aaagaactta
     5881 cctcatttcc aaaagatttt ctgtggggct ctgcttctgc agcgtatcaa gtagaaggag
     5941 cttggcaaga agatgggaaa ggagaatcag tctgggatcg atttgtacgg atacctggaa
     6001 agacatttaa aggaacgaac ggtgatttgg cagtagacca ctatcatcgt tataaagaag
     6061 atattgcttt aatgaaagaa caaggtctga aagcttaccg cttctctgta gcttggacac
     6121 ggatttttcc taatggacga ggagaagtca atcaagctgg gctcgctttt tatgagagat
     6181 tgatcgacga attgattgaa aacgatattg aacctatgtt gactttatat cattgggatc
     6241 ttccacaagc attgcaagat gaatataatg gttgggaatc acgtcagatc attgaagatt
     6301 tcactcatta tgcagaaaca ttatttgagg cattccgcgg gaaagtccat tactggatta
     6361 gtttgaatga acaaaatatc ttcacttcat taggttattt gttagcagca catccgccag
     6421 gtgtgacaga cccgaaacga atgtatgaag ttaaccatat tgctaattta gcaaatgcat
     6481 ctgtcatcaa taaatttcac gagttagaaa tgcctggaaa aatcggacca agctttgcgt
     6541 atacgccaaa ttatccaatt gacagtaatc ctgaaaatgt tctggctgct gaaaatgcag
     6601 aagacctgat ggccaattat tggatggatg tctatatgtg gggaaaatat ccgattgctg
     6661 ccatgaggtt cttagaagaa aaaggatggg caccaacgat tgaagctggc gatgcagaat
     6721 tgctagaatc tgctaaacca gattttcttg ggatcaatta ctatcaaacg gcgacgaatg
     6781 cattcaatcc attaaacgga gttggtgctg gaaaaatgaa tacgacagga aaaaaaagtt
     6841 catcagaaga aactggagtt cctggaatgt ataaaaaagt tgagaatcca tttgtcgaac
     6901 gaacaaattg ggactgggag attgacccag aaggattaag gatcggactg cgtcgtatca
     6961 caagccgtta ccgcatgccg gtactgatta cggaaaatgg cttaggagag tatgacaagt
     7021 tgacagaaga taaacaaatc catgacgact atcgaatcaa ttacttacaa agccacgtca
     7081 aagccatcaa agaagcaatt tcggatggag cagaagtcct aggctattgc acctggtctt
     7141 atacagattt gcttagctgg ctgaacggtt atcaaaagcg atatggattt gtatatgttg
     7201 atcaagacga aacacaagaa ggatctttgg aacgatataa gaaagacagt tattactggt
     7261 accaaaaact gattgaagaa aatggagaga acctttagtt cacatactta aaacaagaag
     7321 gtacggctgg atatggccgt gccttttctt gataaaatca tttagaaatt aagaaaaact
     7381 gtaaaataag ctgaaaaatg aaagaaagcg tcagttcttt caaacaaaac tttagttccc
     7441 ttactttttt ttgtataatg aaacttgatg aaatgaggga taataaatgg cacaaaagaa
     7501 acggcctact aaaaaaagga aaaagacgaa aaaaacgaaa aagcaaaaac agcagcagga
     7561 acaaatgctg acgattggtg ttgggtttgc cttgatttta tttgctgtct tcggcttttt
     7621 aaaattaggt tttttaggaa tattgattgc aaacggcttt cggatcattg caggaaatac
     7681 ttaccaaatc ctctgtcttt tattagccgt attaggattt tggattgtca ttaagaatac
     7741 agagttttca attggtaaaa accgacgctg gtttggcggc atactctttt atttcggtat
     7801 cttattattg ttacatgccc atttatttgg caaactccat actggggaac caaatatcat
     7861 ggggacgaca tgggatttgt tagccagtga tataaaacaa agtcaagtag acaataacgt
     7921 tggtggtggg atgattggag caatccttta tcattttact tacttcttga ttgcacagcc
     7981 tggaagttat ttagcagcag tgctattact ggcaggagga gcattcctgt tcagtaatct
     8041 ggaaggctat cagctgttaa atggtatcca atcaattgga gaacgagtac aggaattatt
     8101 ggaaggtgat cctgccaagc aagctcgaaa gcaagcggca aaggaagaga gaatgaaaca
     8161 gcgcgcagaa gcgaaagaag caaggcgctt agctgcaaaa gaagctgcag aatatgagaa
     8221 gaaaaaagcg gtgagccagc aaagggaaaa tactgctgat gaatggcagg aaccagaaaa
     8281 tacagagccg gagcagctaa gttttgttcc aatcgacagt tttcaagaaa atatccatcc
     8341 tgcgaatcta gagaaaccag taccagatac accaaaacag accaatacag cagaaggttt
     8401 ttcagatgag ttgccagagg atgacggtac ctcattagaa tttgagattg aagcagaaca
     8461 ggaaaatcaa gactatgaat taccgacagt tgatttgctt gattctatac caactgtgga
     8521 tcaaagtgat gagtataaaa aaattgaaaa aaatatcggc gtattagaac aaactttcca
     8581 aagtttcggt gtcgatgcca aggtcgtcaa agccagctta ggaccatccg tcacaaaatt
     8641 cgaagtccag ccggctgtag gggtaaaagt aagtaaaatt gtgaacttaa cagatgatat
     8701 cgcgcttgct ttagcggcga aagatgtccg gatggaagca ccgattccag gtaaatcact
     8761 gattggtatt gaagtaccaa atggaaagat cagtatggtt tcctttagag aaatcatcga
     8821 agcacaacca aaccacccag ataaattact agaagtacct ttaggacgtg atgtttctgg
     8881 acgggtacaa acggctgatc tttcgaagat gcctcactta ctcgtggctg gttcgactgg
     8941 tagtgggaag tcagtagcaa ttaatgggat tatcacaagt atattaatgc gtgctaagcc
     9001 ccatgaagta aaactgatga tgatcgatcc gaaaatggtt gaattgaata tgtacaacgg
     9061 catacctcat ctgttgacgc cggtcgtgac gaaccctaga aaagcagcgc aagccttaca
     9121 aaaagtagta caagaaatgg aagaacgata tgaaaaattc gcggcaaccg gagtaagaaa
     9181 tatttctggt tacaatgaat ttgttcaaca gaaaaacttg gaaaacggaa cgaaacatcc
     9241 aaccttacca ttcatcgtag tgatcgtaga tgaattagct gatttgatga tggtagccag
     9301 caatgaagtt gaagatgcga ttattcgttt agcgcaaatg gctcgggcag cggggattca
     9361 tatgatccta gctactcaac gtccaagtgt ggatgttatc acaggaatca tcaaagcaaa
     9421 tgtaccttcc agaatggcat ttgcggtatc aagcggtaca gattctcgga cgatcattga
     9481 ttctaatggg gcagaaaagc tcttagggcg aggagatatg ctattcttgc caatgggtga
     9541 gaataaaccg atccgtgtgc aaggagcatt catttctgac cacgaagtag aacgggtcgt
     9601 tcaatttgtt acagatcagc aagaagctca ttatgaagag aaaatgatgc cgacagatga
     9661 agttgaaacg acaggtgctc cagaacagcc acaagatgag ctgtttgaag aagcaaaagc
     9721 acttgtcgtt gaaatgcaaa cggcaagtat ttcccttctc caaagaagat ttcggatcgg
     9781 ttacaaccga gcagcacgtt tagtagatga actggaagct catggagttg ttggcccgtc
     9841 agaaggaagc aagcctcgga aagtattcat tgagcaggaa gaaacatcag aaacgttgcc
     9901 ggacgataat gacatgtaaa acttgtgtga tgaagggttg aaaaacgaat aaccaataaa
     9961 aagacaaggc atgatgatac caaaagaaaa aatggttttt ttccatcttt tctaatacgg
    10021 tatgatgtct tgtcttttta gtttttgtga gattaaagtt ctattcgatt tatttgtgaa
    10081 gtattgcatt aaggaaatga atcatgtata gtatgtagcg aaactttgag aaaaagaggg
    10141 aaaatacttt ggaaaaacag tccatttatg gcttgacgaa tgaagaatta atcaattggt
    10201 ttatagaaaa cggcgagaaa aaattccgag ctgcccaagt ttgggaatgg ttgtatcaaa
    10261 aacgtgtaag taatttcaca gaaatgacga atctatcgaa acaattgatc gaaaaattat
    10321 cggagcattt tatcatcaat cctctaaaac agatggtcgt ccaagaagct tcggatggta
    10381 cagtaaaata tttgtttgaa ctccctgata aaaacatgat tgagactgta ctgatgagac
    10441 aagaatatgg cttgtcagtt tgtgtgacga cacaagtagg atgtaatatc ggctgtacgt
    10501 tttgtgcaag cgggctgctc aaaaagaacc gtgatctgac tgctggagaa attgttgcac
    10561 agatcatgat ggtccaacat tattttgatg agcgcaatct tggcgaacgc gtttcacatg
    10621 tcgttgtaat ggggatcggt gagccatttg ataattacga caatgtaatg gactttttgc
    10681 atatcatcaa tgatgccaaa ggattggcaa ttggtgctcg gcacattaca gtttccacta
    10741 gtggattagc acacaaaatc aaggaattcg ctaataacgg cttgcaagtc aatctggcta
    10801 tttccttgca cgcgccgaat aatgaggtgc gaacatcgat catgcggatc aaccgaagtt
    10861 tcccaattga gaaattgatg gaagctgtgg atgagtattt agagaaaaca aaccgtcgaa
    10921 tcacgtttga atatatcatg ttgaatcaag taaatgatag accagagcat gcgcaacaat
    10981 tagcagatct attaaaagat aagaaaaaat tagcatatgt caacttgatt ccatataatc
    11041 cagtcagtga acatgatcaa tattctcgca gtccaaaagc tgatgtattg agattttatg
    11101 atgtattgaa gaaaaacggc gtaaactgtg tgatacgtaa agaacatggt acagatattg
    11161 atgcagcttg cggacaattg cgaagcaagc aaatgaaaaa aacagttgcc aaataatcgt
    11221 atcctactcg ttttttcaat taccataaaa ggagcagttt tcttaattaa ggaactgttc
    11281 aacttaaata agagaaaaga ttctatagcg aaagctataa agcttttttt gacaagaaaa
    11341 accctcatca agcaaagaaa tgtcctcaat ttgatgacag cctgcttgat gggggtttta
    11401 gcaattattt aactgatttt cttatatcct tgctggcttt cttgtgtagc atgataagtt
    11461 tctacgtcaa gaatggcctg tttaaatttt tctcgggtga tttcataagg cacatgttta
    11521 atatcatttg ttgccatggc tttatctaag attgcttcta aatcggtcgt gacattgaga
    11581 ttcatgtcgg ttaacgatac tggaagttgg ttctttttca taaacacata gattttatca
    11641 aacaaatcga actgctgatc catcatcaat aaggtcagta caccataact gacagatgct
    11701 ccgtgaaggt atccctccat tttttctaga acagtcgtac cgtaatagaa tgagtgtgcc
    11761 atagcagaat tatagttatt gttaactaaa acagaaacca atccggtagt aacaatgata
    11821 tccgaaacga cttgttcaat tgcatatgag ggacgatcgt cggcactatc tttcaatgct
    11881 tgcgtaccat aagtcaagag acgatcaatg cacatttttc cgatttggat tcctaatcta
    11941 tccgtgtagc ctagatcatc gccacgagca gaaaaagtag attcgacttc tttggataaa
    12001 gcatcgccaa tacctgccca taagtattta tcaggtgctt cggcaataat tgctgtatca
    12061 atgaaggtgt gtacaggtgg ttttgaagga aagaagagtc cgtccattac gccttcttcg
    12121 ttatagatga cacataccgc agtgacgggc gaacagttcg aaccaatagt gggaaaggtg
    12181 aagactggtt tagctagttt ttcaccaatg acttttacag tatcgcaagc acggccacca
    12241 ccgactgcaa acaggacgtc tgctttttgg acactttcat gctggatcaa ctgttcaata
    12301 tttccataag ttgaattacc cccgtaccag agactggcga caacctctat ttcctgtgtc
    12361 aaagctactt ttattttgtt ttctgccttt gaaagtgcag tcttccctcc aatcagcact
    12421 acttttgtcc catattcttt gacgattcca tttgcttttt catacgcatc tgttccaatg
    12481 gtataatttg gtaagaaaac actttgactc atattcattt ccttctttct tcgttgaaac
    12541 gggttaaaat tttaaagagt gatcgatctg actgcggctg tagatggagt agaggtgaac
    12601 ctaagacagg ataagtgatg aataaggtgg catgaacacc ttgaaatcat caataagtat
    12661 cctaaaaacg aacaagtaca aaaacattga aaacgacaat aatgaatttt tgttcctctt
    12721 agtatttcta ccgattccct ttgctttttt gtaattatct aaaaattcta aaaattaaga
    12781 ttattatatg aaaacaggtt ttatttgtca attcataatt ggtttttttt aaagcaaaag
    12841 ataatctata tcatgatgaa caaacagagt tttttcctcc aattgatggc tttcctgatc
    12901 tttcctattt tgaataaagt gtttctgtat aattttttat tttataagta agataaataa
    12961 tgaaagaata gagatactga ttggtagcta aagatttttg agcgagaaca tttcgttttt
    13021 tcaaaaggac tgttagaata agcaagaaca agggggaaga cgtatgataa tcagtgaatt
    13081 tgaccgtaat aatccagtat tgaaagatca gctttctgat ttactgagac tgacttggcc
    13141 ggaagaatat ggagacagct cggcagaaga agtagaagaa atgatgaatc cagaacgaat
    13201 cgcggtagca gcggtagacc aagatgagtt agtaggattt attggtgcaa tccctcaata
    13261 cggtatcaca ggttgggaat tgcatccatt agttgtagaa agctcccgac gaaagaacca
    13321 aataggtact cgattagtca attacttaga aaaagaagta gcttccagag gaggaatcac
    13381 gatttattta ggtacggatg atttagacca tggaacaacg ttaagtcaaa cggacctgta
    13441 tgagcataca tttgataaag tggcttccat ccagaacctt cgtgaacatc cgtatgaatt
    13501 ctatgaaaaa ttaggttata aaatcgtagg tgtcttacca aatgcaaatg gctgggacaa
    13561 accggatatt tggatggcaa aaacgattat tcctcgacca aattctcaat aataaaaaga
    13621 ggttgtgaca gaagtaatca gcttcaagaa ataagaagga atttacgaaa attacttctt
    13681 gaatttttgt gaaattccaa cttatttccg aagaagctac ttttggaaca ccgtgtac
//
LOCUS       DAZBSA010000074        13698 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000074 DAZBSA010000000
VERSION     DAZBSA010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.074"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            170..508
                     /locus_tag="ACHGMT_002022"
     CDS             170..508
                     /locus_tag="ACHGMT_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835757.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(713..1275)
                     /locus_tag="ACHGMT_002023"
                     /pseudo
     CDS             complement(713..1275)
                     /locus_tag="ACHGMT_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(2007..2588)
                     /locus_tag="ACHGMT_002024"
     CDS             complement(2007..2588)
                     /locus_tag="ACHGMT_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN6835758.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2771..3397)
                     /locus_tag="ACHGMT_002025"
     CDS             complement(2771..3397)
                     /locus_tag="ACHGMT_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFN6835759.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3419..4750)
                     /locus_tag="ACHGMT_002026"
     CDS             complement(3419..4750)
                     /locus_tag="ACHGMT_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFN6835760.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4905..5306)
                     /locus_tag="ACHGMT_002027"
     CDS             complement(4905..5306)
                     /locus_tag="ACHGMT_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFN6835761.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5392..5598)
                     /locus_tag="ACHGMT_002028"
     CDS             complement(5392..5598)
                     /locus_tag="ACHGMT_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835762.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5693..7195)
                     /locus_tag="ACHGMT_002029"
     CDS             complement(5693..7195)
                     /locus_tag="ACHGMT_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFN6835763.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7213..7668)
                     /gene="argR"
                     /locus_tag="ACHGMT_002030"
     CDS             complement(7213..7668)
                     /gene="argR"
                     /locus_tag="ACHGMT_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFN6835764.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7766..8383)
                     /locus_tag="ACHGMT_002031"
     CDS             complement(7766..8383)
                     /locus_tag="ACHGMT_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFN6835765.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8604..9977
                     /locus_tag="ACHGMT_002032"
     CDS             8604..9977
                     /locus_tag="ACHGMT_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN6835766.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10052..11164
                     /locus_tag="ACHGMT_002033"
     CDS             10052..11164
                     /locus_tag="ACHGMT_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN6835767.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(11236..12873)
                     /locus_tag="ACHGMT_002034"
     CDS             complement(11236..12873)
                     /locus_tag="ACHGMT_002034"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFN6835768.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13041..13649)
                     /locus_tag="ACHGMT_002035"
     CDS             complement(13041..13649)
                     /locus_tag="ACHGMT_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFN6835769.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3690 a         2886 c         2452 g         4670 t
ORIGIN      
        1 aataaattgt agccagttca ttttttcttt aactaaaaaa ataatccaag aaacattgaa
       61 cctatggatc caaaagctac ccaaatacta tagctaaccc cccgctacct tcaattttgc
      121 agataaaacg ctttgggaga aaatttcaaa gacaacaggc catcctatca tgcccgatta
      181 taaacaaaaa gctggctatt ccttttgctc tgacaaattt caattgggaa agttcacgaa
      241 actagcaggt gaagaagttt caactgttcg gatcaaagaa tgcccgcttc aaatagaaac
      301 attggttcaa cgaatttcaa ctagacaaga ctttgctatt gtagaacgtg agatcgtagc
      361 tatttttgta gaagaaggga ttttacatga tccaactcat atcaatgtgg ataaatggca
      421 acctcttatc tataaattca gagaatatac aactgccaac cagtcgttaa ctttgaattt
      481 tagattccaa gaattcaaag agtcataatg ataaacatcg ttaggtataa ataaaaaaga
      541 ctcgcagcac gagtaaaaaa caaatagaag ctatacatta gaaaggtcga ctgtgaccct
      601 atagatgcag tatatgcagc ttatgagcat ttcttctgtt ctctttgttt ttgggatctg
      661 tgctgaagtc tttttcgttt tttgccgctc tttctttgaa aaatcgatta tgttatacct
      721 tctccattac taattcaggc atctgctgag taatgatttt tcgcgcaaaa gaaatatctg
      781 ttacccaagg ttcgatttgt tcttcagtca agacaagcgg cattcggtca tggattggtg
      841 cgactgtgcg gttaggttca gtagtcatta tgattgattc tgccattttt tcatcctgat
      901 ggctacgata aaaaccgcca atatacacta ttttttcctt atttgaaaac ataaatttgt
      961 ctttttcttc attccactca ttgggaaaac acaacgattt tgcaaaaatg gttgatgaaa
     1021 ggtctttttt tcttctactg tctctgcacg ggcattaatc atcaattgtc cttttttaaa
     1081 tccggtaaat ccccacttca tgatttccgg agtgaggatg ttttcttcct cttttcttaa
     1141 cgtcacaaca agatttgacg gaaaaacttc tccagctgct aattctgctg cctgcttcgg
     1201 ttgtttcgtc tgggcttctc gataataatt ctgaaggagc tctgactcta gatcaaaaaa
     1261 ataacgacca cacatcctaa tccctcccaa tctttttttc tatcatacac tgcaacaaga
     1321 agttcgcacc aacaaaacgc tttcaatttt tgtagaacta aaaaagttgt gacaagtgac
     1381 aaactctcca gtgaatggta ttctcctgcc aactcctcgg tcacaagtca caatattcaa
     1441 caaacatgag aagctgcttt ttgaaattgt tccttgtgac tcggagtgaa acggcaggct
     1501 tacgacctct gggtaaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg
     1561 caaaaatgta gaaagctatt ttcacaaaat ttttcttaat actcgaaact aagcgacttt
     1621 ttcacaacct cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc
     1681 aaacgaaaaa tatgagcagc tatttttatt tggctgttct tattacttga tgcagggcag
     1741 ctttttcaca acctctccac taaacggtgt tcaatcagct aaccttcggt attagctcaa
     1801 aaaacggcaa aatatgagga actattttgc cgtttgtttc tctaatactc aggtcaaagc
     1861 gctgatttca caacctctaa tacacggtgt tccagaagta gcttcttcga aaaataagcc
     1921 gaaatttcac aaaaatttga gaagcaattt tcgtaaattc cttcttattt cttgaagctg
     1981 accacttctg tcacaacctc taattattaa agaacctttt gcgaagttaa caatctttct
     2041 ttgatcaatt gaaaattctg cctaagtagt gatagatcct gtgtgacttt caacaataag
     2101 atacttccct ggatttcagc aatcaacgct ttggcttgac ggtctgcttc ttcatcggac
     2161 agtttttctt cagaaagtgc caggatattt tgtttcgtat acttgaccca ttgattgact
     2221 aattctagta gcaacagacg gtattcttca tccactgtac tcaactcaat aatcaaatta
     2281 cctacaggac agccatgata agtgatctgt gtctcatgat cagcgaaaat ccagtccaac
     2341 atttcttcta atgcttcttc tggctgtttt ccagcattta agatgtcttc gaacaattct
     2401 gtctgccata cgtccatatg cgcatggatc accgctagac aaacttcttt ttttgaagaa
     2461 aagtaataat aaaattgacc ttttcctgta tcagaagctg ctagaatatc tcggattgac
     2521 gtttcatgat agcctttttt ataaataaga tctttggtcg cttccaagat cttttccttt
     2581 gttttcaatg atattctcct ttttcatttc tcatagtcct atattataca gtaaattcat
     2641 cattttttct tcttaatcgt ttaaaaaaga ctgtgccaaa ttatctgaca cagccctcac
     2701 tataaaactg ttctcatata gctagatgtt cattgattca agaactgcga ggacaaaagc
     2761 aattcctatc ttatgatagt tgctctaata cttcctctgt tgtcatcact tgatctgcaa
     2821 taaagccaaa gttcgtcaac gctgcatcca ttgcttcttg gccaggagca ccaattgcat
     2881 ctgtcgctac cacaacattg aatccactct ctaccaattc acgcatatgg gaatcgctac
     2941 acaagtttcc gttcattcca ccaagaatta ctgtgtcgat tccatttttt ctcaattgga
     3001 gcgccaaatc attgctttct ggtccaaaga ttttatgagg agagctgatg atcgtatttt
     3061 catccaaata aggtttcaat tcttcgataa aatcggctcc tgaaccttct tccactgatt
     3121 catattgact ttttctagca aacatctgct gctcaagcat catatgttct cctgcaccgc
     3181 caaattgcca tttatggtca tgtgggtaaa aataatgagg agaaataaag agtttgtatc
     3241 ctttttcttt tgcttgtttt accatttcaa tcatattttc gatcgtattg tatttttcta
     3301 agatatcttt tgtcaaacca aagccaccgc ctgctggttt caagaactcg acttgcgggt
     3361 cagtgagaac tactgctgta tttttattat tgaacatggt ggattctcca ttcgttattt
     3421 attttaatac atcatttaga gcttcactca ttgtcggatg agaatagatt tgatctctca
     3481 ataacgtata aggaagtttt ccattcatag ctagcgaaat caagttgatg acttcatgag
     3541 aatcttccgc atagatactt gcccctagaa tcaagtcagt ttcagggtcg actaaaactt
     3601 tgaatacacc tttaggatct tctaatactt gtgctttagg gacaccgctt gtcatatact
     3661 tgaataattt gtaattgacg ccttgtgctt tggcttgttt ttctgttaaa cctacattgg
     3721 ataaagtcgg agtgataaat acactgtaag gcacagcttt ccggttattc gttgttcgtt
     3781 tattctcgcc cttcaactga tccataatga ttcgataatc atctagagag atataagtga
     3841 actgcaatcc gcctttgacg tctccaatcg cccaaatgtt gtctgcatct gtacgaaggt
     3901 attcatccac agcgattgct ccgcgatctg ttagtttaac atctgtattt tctaatccaa
     3961 gtgatgctgt acttggtttt cttccagttg cagccaaaac ttttgacgct tggattgtta
     4021 cttcttggcc atcttctgta aaggtgacgg cagctgttga ttcttggtct gctacttgat
     4081 ctacagatac acctagatgg aattcgattc ccgcatttgt catgtcttct aaaatcattt
     4141 gcgcgatatc ttcatcttca cgtggaagaa attcagaatg ggcatcaaga acgacaactt
     4201 tagatccgta ttcattgaac attgatgcaa attccaagcc aatatatcca gccccgatga
     4261 tcactaattt ttccggtaat tgttcctgat ccatcgcttg cgtagaatca atcaagtagc
     4321 gacttgtatt caatcctttg attggtaaaa tgattggttc agaaccagta ttgatgaaaa
     4381 tccgttcgcc ttcaacaatc actttttcac cattattcgt cacttcgatt tgatggttag
     4441 aaagaaaacg tgctgtaccg tccatcactg tcccagttgc ctcatcgctg atcatatgat
     4501 aatttttggc gcgaagcatg cccgtcaatt tttctttccg agcaacagcc tctgtaaagt
     4561 cgattcctct ttctccattg aaaatcaatg atttagaagg aatacagccg atgttgatac
     4621 atgttccgcc atacatacgt gttgactttt caacgactaa aaccgattca ccttttgaag
     4681 ctaacgcctt cgctaaagtc ttaccgccct ttccaaaacc gatcacaata ttttgatatt
     4741 tttccatcat ttttccctcg cttctttttt acctaccgat tggtacaaaa atatgataac
     4801 cgataattgt ctattctcca aatcatctgc tttatttgtc aaaaaaaacc agacaaaaag
     4861 cggtcctttc acgtttttag tctggttcga tttcctttat tcttctaaac aatcgttact
     4921 tctacatcga tatttcctct agttgctttg gagtaagggc agaattcatg cgcatcttct
     4981 gcaatttttt gggcttcttc ttcgctgatg ccatcgatat gcacttctaa tactacaccg
     5041 atctgatagc cgccatcatc taaattataa agagagacac gtgcttttac tgtggactct
     5101 gctgtcattc ctgcttcttt gatcatatgt tgtaccgccc cattgaaaca agaagcataa
     5161 cctgctgcaa ataattgttc tggatttgtt ccttctgcat ggattcctgg aggagtgatt
     5221 ttcatatgca tcgagccatt tggtgcctcg acttctccat tgcgcccgcc atggttgatc
     5281 attgctgttt cgtatgcttt tttcattgta ctgctccttt cgattgacca tttgctactt
     5341 ggctataatt atagtgtatg acaagtaagg cgctttctac aaaaattctg cttattcgaa
     5401 taccagaatg ccgtcttcta ctatttttcc aaaaaacaaa aaaatactac tcgtgaacaa
     5461 cagcgtcata tttgttccaa aataaatagc aatcttttct acataatctt ttatatgatt
     5521 gattttaaga tacatatata ccagaaagtt gaccccataa accaatactg ttaaaaaaat
     5581 aaccatattc gttaccattt tttcctcctt ttgctggtct ccctaccagc actcgattta
     5641 accataccac tttttttgcc gtccaagcaa ctagcctttc aaaaagactg gtttattctg
     5701 ctgttaatga ttcaagatca gaaaaatgtc cgaataattt tgctaatttt actaacgcca
     5761 acttgttctt tccgaattca tcattttcta tctgtaatac tagtaacggc tcatgtaagc
     5821 ttaaacggat caaaaaccag ccgtttccgt atggctcttc tagatcatat cgaatacctt
     5881 cttcattttc cgggtcgaag ttcatccctt tttcttcttc caacagtcgc ttcatttctt
     5941 ctaatatctt gtttccttgt tcgcgtggtt catcgctgaa caattttaat cggatttctt
     6001 gtacttctac gggctgtttc aactctttga tttgttcatc taatgtgata ccttttcttt
     6061 ttaattctgg caaaagcatc aggatctttg cgatgacata tgcaccatca tccagaaaat
     6121 agttttcctt gaatgcagcg tgtccgcttg tttcgatggc tagctgtgta tctttcccaa
     6181 gacggttaag ttctaatgct ttattgatta catttcgata gccggaaata taacgaactt
     6241 ggatgcctcc tttttcttca atgaatcttt gtagatgaga agaagtaggg gaattcgtca
     6301 cgattcttgt tcctggatgc tcttttaata cgattgtact taatactgcg atcaaattat
     6361 ttcgattgat cactgttcca gaagcactga caactgcaga acgatccaca tctgtatcga
     6421 aaatgatccc aagatctgct tcatttgcta aaacagcctg cttgatactt tccatcgctt
     6481 ctttattatc aggatttggg atatgattag gaaatcggcc atctggttcc aaaaattggg
     6541 aacctgttga atccgcacct aagctattca atactttttc agcaaagaat ccaccagcac
     6601 catttccggc atcaacaatg attttccaac catttagtgg tttgctttct agtgttccaa
     6661 tccccttttt aatttttgtg actaaatctt gcgcataagg tgtcaaaagg tctgcttttt
     6721 ctacgcttcc agtttgttcg ctttttattt tttgctgctg tgataggata aaagcaatat
     6781 cttctttttc tgctcctcct tctgaggtaa agaattttat tccattataa taatagggaa
     6841 gatggctagc agtcagcata accgcagcat cgcacgagaa ctgttcaaat tgagtggaca
     6901 taaacatagc aggcgtagta gctaattcga aatcgatgac atgtatccct tgttctttca
     6961 gactttggat caacgcctct ttcagtgctg gtccgcttat cctactgtca tgaccaatcc
     7021 caatcgtcca ttttttattt ggatcggttg atttcttctt tttctttatc cagtttgcta
     7081 gtccccagcc gatctttttt accatgtctg tcgtcaagtt tacttcatat ccttcaactg
     7141 gtaatgcgat tcctcgaata tccgatccat tttgcagcgc tttcatatct ttcatataaa
     7201 agtcctcctc atttattccc tcattttttc cagcttttct gcaaatcgct gtgcttcttc
     7261 ttctgagcgg caaataataa tgaatgtatc tacaccggcc atcgttccga caacttcagg
     7321 gtaagccact tcatctagcc aattcgttac cacatcggca ccatttcgtt cagtcaatac
     7381 gataaccatg aactgaacag actggatacg caaaacctcg cgtctaattg ctgtacgcaa
     7441 acgttcttca tttaaagaaa cagatggctg gttaaataat gtatagcgta ctattttgtt
     7501 ttcattttga ctttttacta acttcatttc tcgaatgtct cgagaaatcg ttgcttgagt
     7561 cacatcggcg cctgcttcat gcagccgcgc aagcagctct tcctgtgtcc caataggttg
     7621 ttctgtaatg atctggcgga tcagtgcttg tcgctgtttc tttttcatat tagacacctc
     7681 tcaatgttga ttatagcaaa agataagtaa tatttcttca tattttggta ttttttactt
     7741 ttattttttg ttttcattca ggttgctaac ctttttctat tttaacacgc aaaatcgtct
     7801 tcattttttc aggtgctgat tttcttggat cgctgatgta aatctctttg tgttctttag
     7861 aagctctttc atagccttct tttgcgatga atgcttctaa tttctcaaac gttgctggtt
     7921 cttcggcata gggcccgacg tgtaagattt gggcacaaag accttcttcc atctttttga
     7981 atactacttg ttctttcaag tcttccggaa gtttgctaga acgctgaatg gcttcttgtg
     8041 ctatcgcttc tgtaacaaag tcaggctgtc ggatcatcaa ttgataagaa aaatgttctt
     8101 ttttcatcac tggttcatgc aaatactttt cttgcagtcc ccattgtcct tccagtggat
     8161 agacagtata catctgataa tcaggaatca gccaattttt tttaggcgac atgcgtattg
     8221 catagctgac tgcatataga caggctactc ttcgtttaaa gtcttcctga ttcgggtcgc
     8281 cgatccctct gatgctaaag aatgattttt taggaacctc tagtatttgc gggctttttt
     8341 tcatactata gctcttgtct ttacgccatt caattttttc catttttctt cctccgcgaa
     8401 attccattta gttatttatt atcatacctc tttttgagtg acttggctaa tcgtgttgac
     8461 actatttttc agtttgtgct agaataagtg aagtaaataa atcgtacaga taacacgttt
     8521 cggcgtgtcg agagagaggc ggtttctatt tttataatag aaatcactct ctttttttac
     8581 gaaatttttt agaggtgatg taaatgacaa aacaaaaaca gctgctttta gtacctgcag
     8641 taattgcaac agggatgatg tcttttgcgg gcgtattgat cgagacggcc atgaatgtaa
     8701 ctttcccaac tcttatcaga cagtttgatt taacgactgc acaagtccaa tgggtaacaa
     8761 cgatttatct tttgatgatt tcgatcattg ttccgctgtc tacttatttg aatcgcaatt
     8821 ttagtctcaa aaaactcttt ttagcttcaa atctgctttt cttggcaggt ataatgatcg
     8881 atttcttctc tcctaccttc atggtcctat tgtttggtcg attgctccaa ggtgcagcga
     8941 caggtatcgc tcttccgttg atgttccata ttatcttgac atttgccccc ctcaatcgac
     9001 gaggcgcgat gatgggtgtc ggtactttga caacggccat tgctccagca attggcccca
     9061 cttatggagg cattatgact tcacatttta catggaatca tatcttcctg tttcttgtgc
     9121 ccgttttgtt tctttctttg attatcggct ggtttgctat tcccgagatg gcagtacaaa
     9181 aaagtggtta tttggattgg ataagcgttt taggaatttg tcttttgttt agtggaagtc
     9241 taacattttt gagtacgttg ggaacagttt ccagctggat tgctttaatc atcggcggta
     9301 ttggctggtt ccttttttac tatcgaagta aaaaagtgga gcatccactg atccgactgt
     9361 ctattctttc aaataaaaca ttccgcttgt ttttgattgg cttcttagtt tatcagtttt
     9421 tattacttgg tgtatctttt gtcttgccga atttccttca gatcgttcaa ggaagtagtc
     9481 cttttgtcgc tggattagcg atgcttcccg gcgcagcggt tggtgctttg ctttctcctt
     9541 tttccgggaa aatgctggat caatatggtc cgaagaagcc aatttttgct gggctgattc
     9601 tttctttgat tggttggcta gcattattcc tactcatagg acacgcttca gtgggtgtat
     9661 tgattgcttg tcatgtgttt tatatgatcg gtatcggatt atcttacagc aatatgatga
     9721 ccaccggaat gaatgcactg aatgaagaac tacaaggaga cgggaacgca atgttcaata
     9781 ccttgcagca gttttctggc gctgtggcta cttcattagt agcggtcatt atcaattacg
     9841 tccagcatca tacttcccat aactatgaag tgtctactac attaggatca aaagcagcat
     9901 taggcgtctt gttgctgttg cttctcgtaa gctttgctcg attcgcctat tatctgttct
     9961 ttgctaaaaa agcgtagatt ccatatacgg caggtgttgc atctgccgtt tttttgtgtg
    10021 aaaatggttg tttgtaaaag gaggcttttt tatggaaaaa attgccatta tcggtggcgg
    10081 gatcattggt atgaccctag ccagttactt agatacatca aaattcaatg ttgctttatt
    10141 tgatgaaggg acaggacagg caactaaagc tagcgctggg attatctccc cttggttgtc
    10201 gaaacgcaga aacaaacaat ggtatcgtct ggctaaagac ggcgctgctt ttttccctaa
    10261 acttataaaa gatttccaac ttgatcaaac gatttacgca caatcaggga cacttttgct
    10321 tcgacctgaa aaagatttag ctgaattggc tgttcttgcc gagaaacgaa aagaagaagc
    10381 gccagaaatt ggaaacatcg aatggctttc acctgaacag acatctcaag cacttccgtt
    10441 attgaaacca gcagtttcac tgcgtatcag tggtggtggt cgtctggatg gaaaagcata
    10501 tttagaacat cttggtcagc gtttagcaca aaaacaaatg gagcagatcc aggcaaaagt
    10561 cactttgcat aagaagagcg attcttggct catcaaggga ccgtttggag aatacacagc
    10621 tgatcgtgta attttgacac ctggcccggc acttaagcct ttattacaag aattaggcta
    10681 tgctgcagat atacgtccgc aaaaagggca gctcttagca tttcagacat cttataagat
    10741 gagtcaagac tggcctgttg ctatgcttga cggagaagct gatttcattc ctttcatgga
    10801 tggaaagatc cttttaggtg ctacccatga aaacgatcaa ggctgggatt tggctcctac
    10861 tagcgaagct tataagggat tgactaagag tacagaacag ttcttgactg atccagatca
    10921 gataaaaaac tggccatacg agttccgtgt aggtacccgt gcttatacgt cagatttttc
    10981 cccatttttc ggcgtactgc cagatgatcc aagcttactg gctgctagcg gtctaggctc
    11041 ttctggcttg accacaggtc cttatatcgg ctatctatta gccaacttct taaatactgg
    11101 tcattggcct acggaagatt atcaaaagga catctccacc tatattacga aaaatccatc
    11161 atagaaaaac agctttttca agtaaggatc ccgagcgata ttgcgcatgg attacttctt
    11221 gaaaaagctg ttattttagt tcaatttctg aatcaaattc tccaaacttt cttttgcttc
    11281 atctggcaag agatcttttc ctatctgagc ttcaatgaat cgtaaacgaa tatcaagtcg
    11341 ttgtttcacc agatttttat aggacgtatc ctcaaaagaa acaggaaaat ctggtatagg
    11401 taaatagtgg atgtcttcaa atggaccaaa cgacttgaat tctgccgttt gttgcacgat
    11461 tctttcaacg attgacaatg tcgtttcagg tttgattttt ttatcatcga aatatccagt
    11521 attcaggaca tagcaggctg tttgctgttt caaaaacaac tctcgaaaag cttgatagtc
    11581 ttccctaaga ggatagacac ggaaaggatt agcaaatggt tcaatgacta gctcatctag
    11641 ctgtcttttt gtagtgatat tttccgcgtc tgatcgtttt gttgctaatg ttgcaccaaa
    11701 agacgctgct aaaactgggt cattgatctt gataattggc ggaagcgaat catctttcat
    11761 gatccaaaaa acagcatcga tcttttcgct aagatgatcg actcggtcgg gtgtcgcata
    11821 acgtgatttg actgtccgcc catttccgtt tcgtatatct cctgttacta ggactttttc
    11881 tcctttactt gtacatgtca cgcccacatt ctgacaagta aggaaagaag aaatggccgg
    11941 atcatcagac ggatagtctt gtgttttatc aaagtaagaa ggttccaagg cagtagcaaa
    12001 accatttttt cgagaaataa cgaaagcatc gtcatgcaag atcgaaacag agtgaccttc
    12061 cgtatgtttt gctaaggtaa tcgttgattt tccagaaccg gacaacccaa atacagccat
    12121 cgtataggtc tggtcattac tagcatattg tttcatccca ccgtggcagg caataaagcc
    12181 attgcgattt cccaatgccc aagcaagcgt caaagtcgct ttttttaatt ctccaaagta
    12241 acgtaatcct agcacagccg ctgtattatg gagagcatca aacaaaatga gtccatttgg
    12301 aaatgtttca tgtgtccatt cgggatcaca aaagatataa agatctccct catcaaagga
    12361 aacagacttt tgataagtct ctaagatctt cgaattcgct ggctgaaaat tcagcaaata
    12421 agaatacaaa ttgttctcaa atccttctgg aatcagcaaa tgactttgca cagaaaattc
    12481 ttcagatagg ccaacgatta cttttgtatg gtaaaactgg cgcttgcgac tttgatacac
    12541 agcttctctt aaaattttct cgtatttgct ttgttccttg tctaagtcct caaagatccg
    12601 ccgagcactt gctgttcttc ccacaatgct tccatcattg aatactagtg tcctaacatc
    12661 agctggtaaa tctagttctt cagcatgtga gattgttaaa tctgtgatga tcgttcctgg
    12721 actccctttt gctagttgat aagctgtctt cctatcaaaa acttcatgaa cattattacc
    12781 gtaaaatgtc gtttctacaa tcgatcgaat actggataac agtggatttc cacttgtgat
    12841 ttcgtttaat tcgagactct cttttgtact catggctcct cctcattccc taaacaaaaa
    12901 aacgttttct ttattataca ttattcgagt ttttgtttgt actgctgcgc tgtgggaagc
    12961 aaacgagtta tttttcacac aaaaaaaccg agaacttgaa taaacaagtt tctcggtttc
    13021 atcattttcc ttttatcggc ttagtaccag ccgtttgcta accagaatgt tttagcagca
    13081 tcccatgaac cataacgaga tgttacatat tgttctgcta cacgttcttg attggcagct
    13141 gaatagtcgc cgtttaagta tgaagcatct aattgataac ggccgatgta acgtccgtta
    13201 gtagctgtgt atgaaccgcc tgattctttt tgtgcgatcc attcttttgc agagcttgaa
    13261 gttggcgctg ttgcagcagc tggggcagca ggagctactt caactgtttc aacaggtgca
    13321 acgtatgtat gttcttgcgt tgctgtttca gttgtagatg ctgtattttc tgttgttttg
    13381 ttttctgttg ttgctgaaga atcatttgaa gttgggattg ttaattgttc accaacataa
    13441 atgcggttga tgtttgcgat cgaatttttt tcagcaattg catcgatcat tgagttgtct
    13501 cctgcaaatt tttgtgagat cttagataat gaatcacctg attttactgt ataaacttca
    13561 tcagcgtgag catttgtagt tcctacaaac attgcagtag caccagcagc aaaagttgta
    13621 cctaaaacaa ttttcttaaa tgaattcata ttaaactcct ttttttctcc gttttttatt
    13681 ctttcgacgc tattaact
//
LOCUS       DAZBSA010000075        13532 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000075 DAZBSA010000000
VERSION     DAZBSA010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.075"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..746
                     /locus_tag="ACHGMT_002036"
     CDS             99..746
                     /locus_tag="ACHGMT_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFN6835770.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKLLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="ACHGMT_002037"
     CDS             1139..3784
                     /locus_tag="ACHGMT_002037"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFN6835771.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="ACHGMT_002038"
     CDS             4094..5407
                     /locus_tag="ACHGMT_002038"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFN6835772.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="ACHGMT_002039"
     CDS             5397..6050
                     /locus_tag="ACHGMT_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN6835773.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /gene="radC"
                     /locus_tag="ACHGMT_002040"
     CDS             6105..6797
                     /gene="radC"
                     /locus_tag="ACHGMT_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFN6835774.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="ACHGMT_002041"
     CDS             6947..7147
                     /locus_tag="ACHGMT_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN6835775.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7401..9080)
                     /locus_tag="ACHGMT_002042"
     CDS             complement(7401..9080)
                     /locus_tag="ACHGMT_002042"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFN6835776.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9082..9294)
                     /locus_tag="ACHGMT_002043"
     CDS             complement(9082..9294)
                     /locus_tag="ACHGMT_002043"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFN6835777.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9687..11417
                     /gene="efrA"
                     /locus_tag="ACHGMT_002044"
     CDS             9687..11417
                     /gene="efrA"
                     /locus_tag="ACHGMT_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFN6835778.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11414..13174
                     /gene="efrB"
                     /locus_tag="ACHGMT_002045"
     CDS             11414..13174
                     /gene="efrB"
                     /locus_tag="ACHGMT_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFN6835779.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13269..13466
                     /locus_tag="ACHGMT_002046"
     CDS             13269..13466
                     /locus_tag="ACHGMT_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835780.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
BASE COUNT         4292 a         2350 c         2911 g         3979 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag ttgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt atttttttgt
     7261 atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca aggaataaga
     7321 acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca ggacaaggtg
     7381 ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg caaaatcatt
     7441 ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat cgcttctttc
     7501 aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg gaataagatt
     7561 cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc acgagagaga
     7621 atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac agctaataca
     7681 agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat atcaccgatc
     7741 ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg cgcagaatcg
     7801 ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc aggaacacct
     7861 acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg gacaggaata
     7921 aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc accgtgaccg
     7981 gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc agacagcaaa
     8041 ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga aaagatcact
     8101 gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg
     8161 gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc aggcaagcga
     8221 ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc taaacgttga
     8281 ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg accagttgcc
     8341 acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg
     8401 atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt tttttctgat
     8461 ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa gactccttct
     8521 tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt gaaatcgaat
     8581 ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat ccctaatgca
     8641 tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa acggatgatc
     8701 gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc atctaatttg
     8761 tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc ttgtttcagc
     8821 aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa ggcttttact
     8881 ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat tccaagtaaa
     8941 tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg gaattgtacc
     9001 ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc tgtctcgtta
     9061 tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat tttctttttc
     9121 gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg gggtattttc
     9181 ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag attttgtttc
     9241 ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga tcattgtttt
     9301 gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga caacacaaaa
     9361 cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga ttcgagtcaa
     9421 agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta ccttctgtat
     9481 aaaaaatata caacaattaa ttgttattct agcataaaac agcaaataag tatagaaatc
     9541 aactagattg aaagaaaaaa tcaatgttta tcatcccgct ataatcataa tgactttttt
     9601 atgagagaag tgttctatta ataggctcgt tgtgctatca tttttagtaa tgaaaatgta
     9661 acaaaagatt aaaaggagat gagaaagtga aactaatgtg gcattatacc atgcgttata
     9721 agaaattctt gtttttcaat ttcatatgcg tatttggttt tattttgatt gaattaggac
     9781 ttcctacgat tttagcacgt atgatcgatg taggaatccg caatgatgat tttgattatg
     9841 tgaagcaaca agggatattt atgatcgtta ttactgtaat cggtattatt ttgaatatct
     9901 tgttaggtta ttttggttcg aggattacga ctaatatcgt agcagatatt cgagatgatt
     9961 tgttcgaaaa gatccaaagt tattcgcatc aggaatacga aaatctaggc gtatcttcat
    10021 tgatcactcg tacaacaaac gatgcttatc agatcatgtt gttcctgcaa aatattttac
    10081 gtatcggttt tatggcacca atgatgttca ttgtcagttt gtacatggtc atgcgtacaa
    10141 gtgtgacact cagtttgtat gtagtaggtg cattaccttt actattattg gcagtcgtag
    10201 cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca aaaaaactta gataaaatca
    10261 acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat tcgtgcattc gtcaatgaaa
    10321 agtttgaaga aaaacgtttt gctgtagtca atgagaatta tgcttctagt tcaaaaagct
    10381 tattccgttt gatggctgtt gcacaaccgg gtttttattt cttatttaat atcgtaatgg
    10441 tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg cgagcttcgt gtaggagatt
    10501 tgattgcgtt tatcgagtat atattccatg ccttgttctc attcatgtta tttgctagtg
    10561 tgttcatgat gtatccaaga gcagctgttt cagctggacg tatccaagaa gccctagatg
    10621 ccaaaccttc gattgtagaa gatccaaatg gtatatctga tacaaaaact aaagggtata
    10681 tcgaatttca aaatgtaaca tttgcttatc caggccattc gcaagagcca gtgatacgaa
    10741 atgtcagttt tacagcctct ccaggagaga ccgttgcttt tattggaagt accggtagcg
    10801 gaaaatctac gttgatccaa ttgatccctc gcttttatga tgtgagtgaa ggaaagatct
    10861 tgatcgatgg agtagacgtg agagattatc ggctaagcaa gttgcgagat aagattgggt
    10921 ttattccgca aaaagcgctg ctatttactg gaacgattgc agataatttg cgatacggga
    10981 aagaagatgc tacgcaagaa gaaatggaac gtgcagctga gattgctcaa gcttcagaat
    11041 tcatttctcg aaaaccagat ggatttaatg aacatttatc agaaggtggt tccaattttt
    11101 caggtggaca aaaacaacga ttggcaatag ccagagccat catacgccgt ccggaaatct
    11161 atatttttga tgacagcttt tctgcattag attatcaaac ggatgcgaag ttacgtgctc
    11221 gtctgaaaaa agaaacgaca gaatctactg tattgatcgt agcacaacgt gtgggaacga
    11281 tcatgcatgc agataagatc atcgtattaa atgaaggaga agtcgtcggt atggggacgc
    11341 atcgggagtt gttggaaaaa tgcccgattt attatgatat tgccgcttct caattatcaa
    11401 aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa cgtttagctc gatatatcag
    11461 accgtatcgt gagacttttc ttttagtgat cctatttaca gttctgacag tggctttcaa
    11521 cgtggctttg ccatatgtta caggacttcc tactacagaa atcagccgta atattgccaa
    11581 tggcgaatca atcaattttt cttatatatt caaatgtctc atttggatca cagttgttgg
    11641 aatagggtat tgtatttcgc aattactttc tggtgttttg atgacaaatg tcgttcaaag
    11701 tgccatgcat gatttgagaa gagacataga agaaaaaatc aatcgtcttc cggtttcata
    11761 tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg acgaatgatg tagatgcagt
    11821 cagcggtgct ttacaacaag cttttattgg tatcgtaaat gcgatattgg gaattacgtt
    11881 ggctgcagca atgatgtttt atatccagcc gatgatggcg ctgatctcta tgatcatgat
    11941 tccaacatcg atctggatct caaaacgagt gatcaatgct tcgcagaaat attttcaaag
    12001 catgcagaat tctcttggtg agctaaacgg ctacgtccaa gaaaatatga caggttttag
    12061 tgtattgaaa gtatatggac gcgaaaaaga aacattggaa ggattcaaaa aagttaatca
    12121 cagcttgaaa tactttgggt ttcgtgcagc attcatttct ggcttgatga tgcctctagt
    12181 tcaattaact gcctatgcta cttatattgg tatggctgtt ctaggaagct tttatgttat
    12241 tactggggcg atcgttgtcg gtcaactaca agcattcatc caatatattt ggcaaatcag
    12301 tcagccaatg gggaacgtga cacaattgtc ttcagttttg caaagtgcat cagcggcaac
    12361 gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa gaaaacaaag tagatgcttc
    12421 actgccagaa acgattttag gagatgtgac attcgatcat gtcgattttg catatgatcc
    12481 gaagaaacca ttaatccaag atttgaattt tgaagtgaaa gctggtcaaa ccgtagctgt
    12541 ggtcggtccg accggtgccg gcaagacgac attgatcaat ctattgatgc gtttttatga
    12601 cgtagacaaa ggagcaataa ggattgatgg aatcgacacc aaatcaatga gtcgcagtga
    12661 tgtgcgttcg ctatttggta tggtcttgca agatgcatgg ctttatcaag gaacgattgc
    12721 agataatatc cgcttcggaa agcttgatgc gacagattat gaagtagttg atgctgcgaa
    12781 aacagcaaac gtagatcatt ttatccgaac gatgccagat ggatatgata tggccatcaa
    12841 ttctgaggga gacaatgtgt ctttaggaca aaaacagttg ctgacgattg cccgagcagt
    12901 ggtctccgat cctaaaatct tgattttaga tgaagcaaca agttcagtag acacccgttt
    12961 agaagcgttg attcaaaaag caatggatcg cgtgatggag ggacggacaa gtttcgtcat
    13021 cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt ctagtgatgg atcaaggtca
    13081 gatcattgaa aaaggaacgc atgagagttt attggcacaa ggtggatttt atgaaaaact
    13141 gtataacagc caatttgctg aggaagcaga ataagtcata taagaataaa acagtttaaa
    13201 acttattttt tgttctctat tcttgataat ggtaaagtaa agttatcaag aaaagtgagg
    13261 acacggagat ggaacagttt ttaaatgaaa aaattactga agccaaaaga gaagttgatc
    13321 gtttaacgca cgtctgtcag acgaagcctt ttgattcagc tgattttttt gaaaatgagt
    13381 ttcaaatcga acgcttggca gcacaaatag aagtgtacga attaatttta aaaaaagtaa
    13441 tagaagaaaa tatagatgta caataaaaac aggtaaaacg tcagttacca gatactgtaa
    13501 atagacatct ggcctgagca ggttttacct gt
//
LOCUS       DAZBSA010000076        13330 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000076 DAZBSA010000000
VERSION     DAZBSA010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.076"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(18..716)
                     /locus_tag="ACHGMT_002047"
                     /pseudo
     CDS             complement(18..716)
                     /locus_tag="ACHGMT_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            795..971
                     /locus_tag="ACHGMT_002048"
                     /pseudo
     CDS             795..971
                     /locus_tag="ACHGMT_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1147..1716)
                     /locus_tag="ACHGMT_002049"
     CDS             complement(1147..1716)
                     /locus_tag="ACHGMT_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFN6835781.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYKTLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1993..3078)
                     /locus_tag="ACHGMT_002050"
     CDS             complement(1993..3078)
                     /locus_tag="ACHGMT_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835782.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFYFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLIKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQALAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFAMNTKKQEDLLGY"
     gene            3405..4280
                     /locus_tag="ACHGMT_002051"
     CDS             3405..4280
                     /locus_tag="ACHGMT_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN6835783.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            4294..4617
                     /locus_tag="ACHGMT_002052"
     CDS             4294..4617
                     /locus_tag="ACHGMT_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835784.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4614..5366
                     /locus_tag="ACHGMT_002053"
     CDS             4614..5366
                     /locus_tag="ACHGMT_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFN6835785.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTAYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5356..7527
                     /locus_tag="ACHGMT_002054"
                     /pseudo
     CDS             5356..7527
                     /locus_tag="ACHGMT_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7639..9810)
                     /gene="copB"
                     /locus_tag="ACHGMT_002055"
     CDS             complement(7639..9810)
                     /gene="copB"
                     /locus_tag="ACHGMT_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFN6835786.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9830..12016)
                     /locus_tag="ACHGMT_002056"
     CDS             complement(9830..12016)
                     /locus_tag="ACHGMT_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFN6835787.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(12016..12225)
                     /gene="copZ"
                     /locus_tag="ACHGMT_002057"
     CDS             complement(12016..12225)
                     /gene="copZ"
                     /locus_tag="ACHGMT_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFN6835788.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12238..12678)
                     /locus_tag="ACHGMT_002058"
     CDS             complement(12238..12678)
                     /locus_tag="ACHGMT_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFN6835789.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQKGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12752..13250)
                     /locus_tag="ACHGMT_002059"
                     /pseudo
     CDS             complement(12752..13250)
                     /locus_tag="ACHGMT_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
BASE COUNT         3737 a         2797 c         2372 g         4424 t
ORIGIN      
        1 aaagaggggg ggatttttta tcgttgtgga atatttaaat accaacgctt gtcatgaaaa
       61 tcttgtgctc cacccttagt atttccttct ggatcgtttg tcgcacgcat catgacgtat
      121 actttcttat tagggaaatc acgcatattg aaagatacat gatagccaac atttcctaaa
      181 gtattataag cttgatttac gtctggtcta taaatcccat cagctcttac tctagctagt
      241 tcttttccag tattgtagtc cataatgaaa atatactcgt atttataatt agcaatgtgc
      301 catccagcta catgcaagtt tgcgttttcg atttctccga actgatcaat gtgggcgtaa
      361 tttgttccat ctgtcagcgt agaatttgca gcacctgctc gagttggatc aattactggt
      421 ttatcctccg aagtagttgg attatcatcc gtaaaaccat gagccaaatc ataggctagt
      481 ttttctttac ttacacccat ttcagaaaga taaccgtaag gatctgtatg atcgccccaa
      541 atattttgtg ttacccataa atgcgatttg attcctggtt ggttataagg agtgtctaat
      601 gttaatggaa taccatattt cattgctgaa tctcttgcca attcaacgta tactttatag
      661 ttcttttcaa aagtttcttt atcatgtgtg tgttgtaact cgatttgcac aggacttttg
      721 gctcacgagg ttgtcctact ctacatcttg ctgatagata tgctgaaagg aaaacaccaa
      781 cattatgttt tgctttttca gctagaacga gacaagcagg aataccttta gaagatatca
      841 aagatttttt aggtcataaa gatgtttcca ctacacaagt ctatgcccat atttctcccg
      901 aagtgaaaaa aagatcaatg aatcaactag aaaactatat agaagagcaa ataaaaaagc
      961 actcaaattg agtgcttttt taagctcact atcactaaaa ctatcactaa ttttatttat
     1021 aagcgaacat gtttctatca ctgatttttt aaatctagcg aaaaaccctt atcttaaagg
     1081 cgttcgttca attctttggc aaggtcttca aagcctggct tgcctagaag tgcgaacatg
     1141 gttttcttat agctcatgta gaaaaatttt cttgatgatg attttatcca tcaattgaga
     1201 ttgactgttt tgctctccta tgatttcagc tatgattgtc cgattttgcg gttttcttct
     1261 taactggtag ttgattttcc cagtttccat caactcgcta agcatatcca cgactccgcc
     1321 aaatccggtc tttttcgtaa taaaacctag tgctaccatt cgttcatcta gattttttgc
     1381 tgtaatcgtc acaccagtaa tgagcgagtg ctgtaatgtt tgggccagct cttccatccc
     1441 ttcagcattg gatacgacgg aagctgtttt gttaataggt tcttcgttta tttcttttgg
     1501 ttctttcctc tccactttca ctgtttgacg aaggacatct agttcttgca agagcgtttt
     1561 gtattgttct tgggtcagca tcacgccagc tactttttca cgattaaata tatatacgcc
     1621 agttcccgcg tctcgcgctt gagcaaatac atccatcggc gatcgtttca cttctgtgat
     1681 cgaagatgtg ggaacttcta atttctttaa ttccaatgga tttcctcctt tactaatcgc
     1741 tttttattat agcacagact gacttgtttt gatctttttt ccaaaggttt tatcggtttt
     1801 tgcctaacca gtaattgata tcggtaagaa gtctttcgat accaaatgaa ggatggtaca
     1861 gatacgtttt ttgatcttcg ttatggtcta gtggaaaaaa atgatcggtg atcactagat
     1921 ctgcataccg aggcggctta tttcgagtac atacatgtat agagatccgt tttcgatgtc
     1981 ttagcgtttt gttcagtacc ccagcaagtc ttcttgcttc ttggtgttca tagcgaaaaa
     2041 aaagacgatt ttcacttcag gagggtggac atctaaatac cctcgttcct ggagtgtttt
     2101 cagtaaaagc atcccccact ctactgcggg gtgtgcaagt gcttgttgga tcgctagccg
     2161 aaattgatat agtttatctt tggaattggg aaatttttct aatgtttcct gataataacg
     2221 aacttcttct atcagccggt catggatacc aattacgtaa cgaagccatt cttggtaaag
     2281 tacttttatc agctttgatt tgctttcgct ctcaaattcg ctattcagcc aaaacagcga
     2341 atcaagcttt tcagtcacaa tcgtcaattt ccgatagtct tgtgtctctt ctatccgttt
     2401 ccaccgcttt tcagtaatct ggttacagct caagcgcaaa attgctagat aatttttttc
     2461 ttttattggc aaaatctgct cctctctaaa tagcaattca ggaaacaatt caaagaatag
     2521 tgttcgactg tctcctaaaa tctcgatttc tgccggatcc tttctttgct tcaataattg
     2581 gatgaacatc tcagaaaagg actgctctcc atcatctgca aaaaaatgcg gaaaatcttt
     2641 caaaaagtaa aacagcagat atcttacttt tctttctgaa ccaaagatct gtgctttttt
     2701 ataagaaaaa gaaaggtggt actcgccaaa ccactttctc aatcgctctt gcgtagaacg
     2761 atcgtacttc tgctcgtaag gttcatttaa tagcagattt ttcagtaaac aaaacggggg
     2821 tgattggatc agaaaaacaa acaagagata aggttcgcta tatccttgat cataaactaa
     2881 ctgaacggtt ttcttatgaa gaatcagctg aatgggacag tcgctttccg ccaaacgttt
     2941 cagatgccga tccactgttt taacatcgat tttcagttct tcagctaaat cagcttttcg
     3001 ccagatcaaa tctttcatta gattgattag tgaaaataaa ttctgtgcat tttttgataa
     3061 tagtttttgc catctcatcg atcatttctc acctgatttc taaacatatt gtttgatcaa
     3121 aatgagttca ctcctaaaaa gataaaaaac tccctttcta tttatttaaa tacgcaaatc
     3181 ttcctcatta ttttttattt tactcaattt aatcgtattc tcatttcaaa acgaatgtac
     3241 tttaaaaaaa cataataaga tcactttgct tgtaggatgt attcccttcc attacaatga
     3301 aataaatagc gattttatga gaaggtgatg tgaaaatgga agaaaaaata gaagaatcca
     3361 aacaaaaaat cgatcagttg atataagaat tagaaaaagc ggtcatcggc aaacgttcaa
     3421 ctatccaatt gatgatcgtg gccctattag caggtggtca tgtcttattc gaagatattc
     3481 ccggtgtagg taaaacgtta ctggtcaaag cactagcaaa agctgtacag ggaaccttca
     3541 gcagagtcca atgtactcct gatctgctgc ctagtgatat tcttggtttt tccgtataca
     3601 attcacaatc aaaagagttt gaatttcgtc cgggaccgat ctttaccacg atcttgttgg
     3661 cagatgagat caatcgtacg actcctcgta ctcagtcagc acttttagaa gcaatggccg
     3721 agaatcatgc aacgatcgac aacaatacat accctctaga caatcatttt tttgtcttag
     3781 ctacgcaaaa tccaatcgag tacgaaggaa catatccttt gccggaagca cagctcgatc
     3841 gttttttatt ccgactacaa atcggttatc cttcttttga tgacgaactt ttgctacttt
     3901 tggataaatt cgaaaaagtg ttggataact taaatgaagt attgaacagt tatgaaatag
     3961 aagaaataaa aacaaatgtg gcgaatgtct ttgtcagtcc cgaagtggca tcttatgcct
     4021 tgcagttagt gtcagcaaca agaagtcatg ctgccatcca gcttggcatc agtccacgag
     4081 gaagcctttc ctttattcag gcagcaaaag cttatgcttt aatccatggg agaaattacg
     4141 tcacgcccaa agatttgcag gatttagttc cttacgtgtt ttcccatcgc ctgatttttt
     4201 acgatcgttc attgaaggaa gacgaaaaga aacaaatcat tcatactctt gttgcacaaa
     4261 ttcctattcc tgtgaggtag attgaggtag acaatggaaa aaatcaaaga ttatttcatt
     4321 aattttgtgt tatttctggt atatttcatc attgtgcttt ttgctcttgt attcaacaat
     4381 tctttaggct ggttcttgtt ggcctttttt actctttatg tatcatggag cttgatttca
     4441 ttaagcagct ttctaaagaa actttcggtt acttgtacat tgcagccact cgtttatcgc
     4501 aaaaaaagta tgcaggttat tttttccata gcaaaaaaga gaactttttc tttacctctc
     4561 cctcgtttac aaatcggctt cccagcaatc tttgcagaaa aaaacaagag attttgattc
     4621 ttacaaataa accgcaagaa gtccaattgc tttggcagcc aaaagaaaga ggatgccatg
     4681 aaacattaac tgtcattttt acagcctatg atagtctagg acttttcaga aaaagtgcaa
     4741 aaagagagat ctcttttcct gtgactgttc tgcctgcttt ttgtaagatt caagcagaaa
     4801 aattacagag agttctggaa aaaaaacagc agctgaacgc gtaccaaaga ggtcttgatt
     4861 ttcgggaaca tcgcgcttat cgtccaggtg attctttcaa ccgaatcgat tggaaattgt
     4921 ctgctcatac tcaagagtgg ttataccgtg aatacgatta tcaagaagaa gaatgttctc
     4981 ctcttttttg cttttggggt tcgcccagtc caaaatttga acctctgcta gacttgtatt
     5041 tttctttatg gcatttgaga aaagaaaagc agccggaact tctgatatta ggcaaccgcg
     5101 cctttcatgg caaagacccc ggtcatacct attttgcttc tttggaagct ggcgatgaga
     5161 aagctttttt cgcacacttg aaaaaatatg caaaaaaaca aatcgtcctc tttgttcctg
     5221 agaattctcc tgaagtctca gaagctattg aaacattgtc aaaagatcga acggcttatc
     5281 ttgtttattt cgtagaaaaa caactcatgg tccaagaaaa agacaagata tgttcattgc
     5341 caggaggtga atggattgac gattaaacgg gaaaaatggc tgcgtggcgt ttactccttt
     5401 gtgatactaa gtgtaggtgc tccgatattt gtagtagcta atcaattgca aaacagctac
     5461 cttcttatcc taactattgg tgtcctatgc cttattgtct gctgtattcc taaaaaatgg
     5521 gtacgtttgc tgctttcttt gccagtaatg ataggttgtt tgtttttata ttttccttcg
     5581 aagacatttt ccatattatg gatcttacag tttttttcgt cagttacaga ggaattcttc
     5641 catctatccc aaggtaaact ttattatttg ccagaaaaga cagctttttt tctcataatg
     5701 ttgctgatct atttatttat tacattgacc gtggattacg actattggta cgcacccttt
     5761 ctttctttga tggcttactt catgatgctt accgtttttc ggaaaaaaga tttactgtac
     5821 gaaatgattg cggtagtaac tgttttgttc gtgtatctgg cagcaaggca atttttctcc
     5881 atccggttgt tgtcgggaaa cactcgattc cagtcattga ctacagttct gcttatttgt
     5941 ttcttaactt ttagcgctat cttgccttta tatcttccga aagtacacca atctctcgag
     6001 gaaacgagtg aaccgatccg agaatatctt aatgatgaag ggctttatga cacactccat
     6061 gagtatcaat taaccggaag tgcaagaaca ggcttcagtg agaatgatga acagcttggt
     6121 ggacctttat atgataatgg cgaagttgtt tttacagcga accaaaaagg aaatggctat
     6181 tggaagatcg aaaataaaaa tcattacacc ggttctggat ggatagaaga tcaggacgca
     6241 cccatccaac caatccaaga attccctgtt gacatcacga gctaccaaac cgttatagaa
     6301 gacaaagaaa acattacatt gaccctaaaa aaacaagctt tcttacctta tccttatgga
     6361 aatactacct tgactgattt ttcattagct agtggacaaa cgctttacta tttgcctgaa
     6421 agccagcgat tcattcctgt cccgaatgaa tcgacacgtt ctgcaacttt acaaacagaa
     6481 aggttagttt ttacgagaga agaattaaaa agcagtcagt taaaagctga tgatttagaa
     6541 aatcagcaga caaatcttca attgccaaaa agtttgccta aacgtattga ggacttggcg
     6601 gtcgaattga ccaaaaatga agcaacttta attcgtaaag tcgaagctgt ctaacagtat
     6661 ttaaaaacaa gcggaaattt ccgctattcc aaaacggata ccgggcatac tccgaaagaa
     6721 cacgattatg tggattattt cctctttgat tcaaaagtcg gctattgtga caacttttca
     6781 acttctatgg tcgtccttct tcgaacactt ggtatcccag ctcgctggac aaaaggtttt
     6841 acaaatgggc tcagaacagg cacagataat caagggaatg aggaatacag cattctcaat
     6901 agtcatgctc attcttggcc agaggtatac tttcctggat ttggctggct tccatttgaa
     6961 ccaacccctt catttgccaa tccaatgggt tcacaaccag ttgctgaaac aaccgaaacc
     7021 gtccagcctg ctatcgaaca accaaatgaa agcgaaaata tggcagaaac tatttccagt
     7081 gagagatctg aggtcattcc atcagaagaa acaacactca caccagctta taaaatcgtc
     7141 ttattcagcc ttggagtgtg tttatccgtc ttgttgttct ttctattgtt attcagaaaa
     7201 tctctgcgct ggacaagtta tcttatctat cttcattttt ttgcttctac taatacgatt
     7261 gcatatcaaa aacttcttaa ccctttggaa acattggtac cgcgcaaacc cgcagaatct
     7321 ttgcaggaat atagcctgcg aatagaacaa gaattcaagc tagatctgcg tttcattcga
     7381 ttaactactc aatatgaagc agaaatatat ggacgacaag cagatccggt aaaattcgat
     7441 aaaaaaacct atttagaaat aacgcgtata ataagaaaaa cggtatacaa aaaaattctg
     7501 acaaaaggga gccgcttcaa aaaataagat aaaatttctg gagaatctac ttctggaatg
     7561 ccgagaacca gctgattgag tatcgattac tcgaaaaaaa ggttgtgaca cgttttttgt
     7621 cacaaccttt tttcattatt atttgatttt caatgtcaaa gcatttagag ctacaactac
     7681 cgtacttaac gacatcaaga cagcaccaac tgctggactt aatatgatac caattggcgc
     7741 aagaattccg gctgctagcg gaattgcaag aatattgtaa cctgcccccc accagaggtt
     7801 ctggatcatt ttctttcgcg tttgcttggc tagatcaaga aaatgcaaaa tatcttttgg
     7861 atcgctgttt gtcaatacga catccgcaga atcgattgcg atatctgtcc ctgcaccgat
     7921 tgccatcccg attgaagcac gggctaaact tggcgcatca ttgatcccat cacccaccat
     7981 gattactttt ttcccttggt caaggtattt ctggacaaca gcttctttat catctggtaa
     8041 taaaccgcca taatattctt cgatccctaa ataatctgct actgcttggg cagcgttttt
     8101 attgtcacca gtaagcatga caggagtgat ttttctttct ttcaatgttc ggataaattc
     8161 ttttgcttct gtttttacta cgtcacctaa agcaacgatt gcagctagat gattttctaa
     8221 aatcaggaag ctgatggtat ttccttgttc ttggtaaggt ttcaataatg cttgttctac
     8281 gtttaatccg agacgctcaa cttctttttc attaactatt ttcacttgtt gttttttgac
     8341 tgttgcctct agcccgactc ctgaaagatt ttttagattt tcagcttgat aaggcttgat
     8401 ttcatgttct ttcaaataat tcataatgcc aacagccaat ggatgattgg cattcgcttc
     8461 caaggctcct agatattgaa ggatttcatt tttattgaac gcatcatcaa gtacttcgac
     8521 acccgttaca gtaaattttc cttctgttag tgttcctgtt ttgtccagca tgatcacatc
     8581 tagatcattg gcttgctcca gtgcattgcg gtttttcaaa agtaatccgt ttttagctgc
     8641 tattgatgtg gagcgagcaa caactaatgg gatagctaaa cctagagcat gaggacaggc
     8701 gatgataaag actgttacca tacgttctaa tgcattagga agatctgtca agaacaacca
     8761 ggcgatgaat gccaggatac ccacaattaa tgcgacataa aacagccatt ttgccacttt
     8821 gtcagataat gactctagct tggatttttc tccttgtgct tttcgcacca tttccataac
     8881 cttcgctaga taaccatttt caccagttcc agttacttcg atttcgatcg ttccatcccc
     8941 attgatagaa ccgccaatca ctttgtcatt ggcttgtttt ttgattcctc ttgattcgcc
     9001 agtcacagct gattcgtcaa caattgtttc acctttcaag attacaccat ctgttggcat
     9061 tttatcacca gaccggacga tcaaacgatc accttcgttt acttctttca atgaaactgt
     9121 ctcttctttt ccatctttcg tcaagcgttt cactgattct ggcagtaatt ctgctagttt
     9181 ttgtaaagca ttgctggcat tcgacacagc attcatttca atccagtgac ctagtagcat
     9241 aataacgatc aaagtagcca attcccagaa gaaatccatg acgtgtgtat gtggattgag
     9301 cagatttgcg ataaacgaat agacactata aatatatgca actgtaatcc ccatcgcgat
     9361 cagtgtcatc atagcaggac ttttttgttt caattccatc ttagctccac ttaaaaatgg
     9421 ctgaccgcca tagataaata gtatagttgc taagatcaat acgagccaat cagaaccagg
     9481 aaacgttact tgaaatggaa attccattcc catcattggt gaaaaaagaa tgattgggat
     9541 agctaggata agcgatagcc aaaatttttg tttgaaattc cccatgtgca tggaatgatc
     9601 catgccgccg tgttcgtgat ccatgtgcat cgaatggtcc atttgtttgc tgtttgctat
     9661 atctccatgg ttatgtccca tgtgcatgga atgatccatc tgactatggt catgtttcat
     9721 ctccatctgt ttttcatgag attctgtact ttctttgttt actacttctt ttttatgctc
     9781 atgttttttc tccatatcgt tcccattcat ttttactcca tcccttctct tactttttca
     9841 tgtgtcgatt taagctcagg gagttcaaca atacactgac ggaactaaac gccatcgctc
     9901 ctcctgcaat gattggattc aaaaagccta gtgcggcaaa aggaatacca atcgtattat
     9961 agacaaaagc ccagaaaaga ttttgtttga tttttctcaa tgtcaaccta gacaactcga
    10021 ttgtttgtgc aatggatgcc aaactgctat tcatcagcgt cacatcggct gtttccatcg
    10081 caatgtccgt tccacttccc attgccatac cgacatcagc taatgctaag gccggtgcat
    10141 cattgattcc atcgcctgcc ataccgactt ttttgccatc tttctgaagt ttttctacgt
    10201 aggctgcttt atcctctgga agtacttcag caaagatatg ctttggatca ataccaactt
    10261 gtttaccaat cgtttcggct gccagtttgt tgtctcctgt cagcataaaa acatctaatc
    10321 ctttattctg gagctgcttg attgcttgtt tggcttctag tttgatttga tcagccacgg
    10381 caatccaccc gatcacttgc ttttcatcag ctaaaaacat gactgttttt ccctgctgtt
    10441 ctaattctaa cgcgtgttct tggtattcat caaatgatag gttcaattca attaatcgtt
    10501 tacgtgtacc cgcaaaatag cgtactccat taatcgtacc gctgatccca gcacctgggt
    10561 gggctgtaaa atcaatcata tcgtatgttg ctgcttcttg cagttttccg tattccacga
    10621 ttgctttacc tagtggatgt tcagaagctt gttctaaagt ataaaaaata gataggactt
    10681 cttttgaacc gactagatct gtaactttgg gctttccttc tgtaatcgtt ccagttttat
    10741 ccaaaacaat gctgtctaag tgagcagctg cttctaatgc ttctccgcct ttaattaaaa
    10801 ttccatttcg tgcaccaaca cccgttccta ccataatagc tgttggggtc gctaaaccta
    10861 atgcacatgg acacgcaatg actaatacag agacactatg gatcaatgcg agttgccaat
    10921 caccagtgat caaacctgta gctatgagcg tgataaacgc gatgactaat acgatcggaa
    10981 caaaaatccc tgagatccga tctgcgattt tttggattgg ggctttgctc ccttgagcat
    11041 cttccaccat ttgaatgatt tgcgctaata ctgtctgctt gccgacttgt gtgacttcca
    11101 tgtgtagcag accattcgta ttgattgtcc cgccaaaaag cgtgtcatcc ggattctttt
    11161 ctacaggaag attttcacca gtaagcatac tttcatctat cgcactgctt cctgaaataa
    11221 ttcgaccatc tgctggaatc tgctcaccag gacggatgac tagttgatca ccaacgacta
    11281 cttcttcaat cgctaacgtt tcttctttcc cgtttcttat cacttgggcg gtttttgttt
    11341 gaagagacat caattgtttg attgcattac ctgttttcgt ttttgctgta tgttccaaat
    11401 atttgccgag caaaatcagc gtgatgatca tactgctgct ttcaaaataa agatcttggg
    11461 ggtgtccacg gaaaaatccg ttgtaaatgc tcaatgcaaa ggcagccgat gttccaattg
    11521 ctaccaatac atccatattt ggtgctttcg tcttgattgc atggtacgca cctttataaa
    11581 aacgagcacc gacataaaat tggacaggag cagacagcac taactggacg attggaaaat
    11641 gaaaaaagtg aacaatcgcc gcatggcttc ctaacatcat agcaatcatt gacaacatca
    11701 acggcaaagt caaaaccgta ctcaatataa gatcgaatag catttttttc aaatacgctt
    11761 gcttttcttc tgcaattttt tgtttgtggg cttcatcgta caaaattgca ccataaccga
    11821 tgttttctac gctttggatt aatttttcag tggtcgtccc ttcatatttg acacttgctt
    11881 tttcagtggc caagttgact gtggcattga ccacaccagg ttgtccgttc agctcttttt
    11941 cgatcctagc agaacaattt gcacaagtca tacctgtaat gacgaacgta tccattttgg
    12001 tttctgctgt tgccatcaaa tcacctctgc tttataaccc aatccattga ttgcttgaca
    12061 aatcttttcg gattgaactt ttgtttcatc aaacttgacc gtaccgtttg cttttttcaa
    12121 atttactttc actttttgca cgccgtcaag tgcgcttact gtttcttcta ctcttgctgc
    12181 acaatgattg cagctcatac cttcgattga aaattgttgt ttcatttttt cttcctccta
    12241 ttgatgttgt ttacattcac attgacctgg gatacagtta cattcgatcg tatctactgg
    12301 ttgcttttgt tccagttgtt tcaaaatctc attgatatct tcttgagtca aagtagcttc
    12361 ttctactaga tccgcaatcg tctctccaat cctttttgca cagatatggg aaaaaagatt
    12421 ttccgtcgca ctgcgaactg tttccatctc cgagacagct ggatgataaa tgaatttttt
    12481 tcctttttgt tctgtccaga tcacttcttt ttttaccaaa cgtcccagca aagttttgat
    12541 cgttgctggt ttccaatcca tcgaatcagc taagatctgc gtgatttctt gggctgtcgt
    12601 ctgtccaagt gtccaaataa cacgcatgat ctcccactct gaatcactga tctttactgg
    12661 cgttttgact ttttccattt atttcacacc tttcgtttac aaatgtaatc attagcttat
    12721 gaatacagat tacgcttgta aacaaaaact gtcaattatt tttgctctct attttcaatt
    12781 tagttaaaaa ttcttgtttc tctcgtcttg ttttaaggat gatcagttta tttcttgcat
    12841 tagctacttg gatcagctgc tgtatttttg gttcattgtt tccttcaaaa tgccagataa
    12901 aagataaaaa ttctaagtat ttcctgttta gtttctccga aaatgattca ggcatatctg
    12961 gccgagattt tttgtttatc tttcttttta gagaacgcca aactactcgg tagatggcga
    13021 gataacgcgg cactttaagc caaatgatct tatctgcttc tttaatcctt tcctctaacg
    13081 tagaagtata atttccttca acgatccatg acggattaga tactataaac gattgctgtt
    13141 cttgcaaaaa ccactttttt gcctgaatgc ggaaagccgg tgatttcttt gatcttcctt
    13201 gcgagggacg attttcccga cccactttgt ccaatcacga tgatcttcat tcttctccct
    13261 cacttctctt tggaatttgg ttcgatcata ccacttataa cgccgcaaaa aaacaaggga
    13321 ctgtgacaaa
//
LOCUS       DAZBSA010000077        13260 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000077 DAZBSA010000000
VERSION     DAZBSA010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.077"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2373
                     /locus_tag="ACHGMT_002060"
     CDS             <1..2373
                     /locus_tag="ACHGMT_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFN6835790.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2434..3894
                     /locus_tag="ACHGMT_002061"
     CDS             2434..3894
                     /locus_tag="ACHGMT_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFN6835791.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3905..6109
                     /gene="topB"
                     /locus_tag="ACHGMT_002062"
     CDS             3905..6109
                     /gene="topB"
                     /locus_tag="ACHGMT_002062"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFN6835792.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6125..6274
                     /locus_tag="ACHGMT_002063"
     CDS             6125..6274
                     /locus_tag="ACHGMT_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835793.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6349..6942
                     /locus_tag="ACHGMT_002064"
     CDS             6349..6942
                     /locus_tag="ACHGMT_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFN6835794.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6955..7854
                     /locus_tag="ACHGMT_002065"
     CDS             6955..7854
                     /locus_tag="ACHGMT_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFN6835795.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7857..8354
                     /gene="ssb"
                     /locus_tag="ACHGMT_002066"
     CDS             7857..8354
                     /gene="ssb"
                     /locus_tag="ACHGMT_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFN6835796.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8720..8980
                     /locus_tag="ACHGMT_002067"
     CDS             8720..8980
                     /locus_tag="ACHGMT_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835797.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9425..9631
                     /locus_tag="ACHGMT_002068"
     CDS             9425..9631
                     /locus_tag="ACHGMT_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFN6835798.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9652..9882
                     /locus_tag="ACHGMT_002069"
     CDS             9652..9882
                     /locus_tag="ACHGMT_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835799.1"
                     /translation="MTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLVEDAPTML
                     FEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            9898..10335
                     /locus_tag="ACHGMT_002070"
     CDS             9898..10335
                     /locus_tag="ACHGMT_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835800.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10328..11035
                     /locus_tag="ACHGMT_002071"
     CDS             10328..11035
                     /locus_tag="ACHGMT_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835801.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11199..11399
                     /locus_tag="ACHGMT_002072"
     CDS             11199..11399
                     /locus_tag="ACHGMT_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835802.1"
                     /translation="MNKIIPLLFVLCIGEILMRHGAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKLIAAIFFIGLFR"
     gene            11415..11594
                     /locus_tag="ACHGMT_002073"
     CDS             11415..11594
                     /locus_tag="ACHGMT_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835803.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            11919..12182
                     /locus_tag="ACHGMT_002074"
     CDS             11919..12182
                     /locus_tag="ACHGMT_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFN6835804.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12176..12526
                     /locus_tag="ACHGMT_002075"
     CDS             12176..12526
                     /locus_tag="ACHGMT_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFN6835805.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(12550..13164)
                     /locus_tag="ACHGMT_002076"
     CDS             complement(12550..13164)
                     /locus_tag="ACHGMT_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN6835806.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
BASE COUNT         4902 a         2193 c         2729 g         3436 t
ORIGIN      
        1 atggcagaag aaaaagaacc actagacaat agtcgtttgg gaaaatccaa aagaaaacta
       61 gtacgattgc aaaatgaatt aaacgaacaa attgaaaaga tgtttgaaca ccaaaaaatg
      121 acgaacggtc aaccaatgaa cgacaagagg aatggccgtt catggtttaa ccagcaagaa
      181 cgaatcgaaa ataaagtcca aagtctgaga gaagagatca agcaacaaga agaatacgtg
      241 gaaaagcttg aaagacaaga agagctaaag gaaatgggct ataacaagta cggcggactg
      301 gatatgacga tcgataatat tccaagaatc aaagaagaaa tcgaacgctt tgaaaaaggt
      361 gaatcggctt tttcagccgc cacgatccga aaataccaac ggaagttgga aacattagaa
      421 cagctgaaag aaaaatcaga aaaaggaaaa gaaaatatcc tccctgaagt acaagcgatc
      481 attgacagcg gacgagtgac gcaatggaaa aaaaatccaa ccatttattt tttgaaaggc
      541 tatcgaaaag ttgctttaga actgaatgaa aacgggggat ttgaggaatc aaaaaaatac
      601 caagccacgt cggaagagga aaaagcagct gttcgtgaat tacttgaaga ggctagacca
      661 actgattcta tgaaggagaa agaaaacgat catttctatc gagtagaatt caacgaaaat
      721 gatccagaaa aaggattgaa aagttatggt ggggagatcg taacattcga attgatcgat
      781 caactcaaaa cattggataa ccaatacgcc gcagaagcag gttattataa attctatttt
      841 gaggaagtga agaacggaga agtagtcgat cagatccgaa tggatctagg ggatggcgtc
      901 aacagtaatc aaccaatcta tttagagtta gcaaaattgt gcgaacaagt acctgagaag
      961 gaacaaaaac aatcggtcaa aaatgaacct gtttcgaaag aacaaatgca gtcgaaagaa
     1021 acaaaaacaa aacaaaaggg ctatcaaaaa cgaacggcag aagagattaa acaagaagtt
     1081 aaatcccttt cgcaacaggc actggatgcg gtgaaaaagc atacccattc tcctgaagac
     1141 gtcaaagaac ttttgaactt tatgtctcgt tttccagaac gatcctttag aaatcaatta
     1201 ttgattgagg aacaatttcc aggtgcaacg gcttgtttag gtcgtgccgc gttgaaaaaa
     1261 gaaggaatct atatcaaaaa aggggaacaa gggaacaaga tctttgtcag aaaaacggtc
     1321 aaagggtttt atgagaaagg acgtggcttt gttcgagaaa cagaagctac accagaagat
     1381 aaaaaacgaa tcgaaagtgg tcaaatcgaa gtgatcaaaa aaccttacta tacgattgaa
     1441 aaagtgtttg atatcacaca aacacagtta aaacctgaag actatccgaa aatctttccg
     1501 aaccgtgtct ttgattttca attggataaa aaaggacagt cagaacttca agctgggatt
     1561 caagcggtag ccaacaatat tggaatacaa attcgagata tgacagaaag tgaagtctat
     1621 caaagagaat taggacaagc acgtggggcg tatgttcgta acgaatttac tggcgaagaa
     1681 gaaatcgtca tgaatactcg aaatactcct acgcagcatt tagctacaag tattcatgaa
     1741 ttagcccatg caagaatgca caaattttca gaattagata ccgcaactaa agaattgcag
     1801 gcagaaatga cttcttatat tgtgtgcaaa cattttggaa tggacacgtc ggagaaagct
     1861 attccttata ttgcagcatg gacgaagaat ggtcagacgt tggatgataa ggaagctgcg
     1921 gaacgtggga aaatcatgaa tgatgtcagc cgtgtcgcaa atgaattcat tcaaaccatt
     1981 tctaatgaaa tcaatcaata cagagaaaga gaaccagaga tccaaccagc agaagtgaag
     2041 caggagcata aaaatacttt agaaacgcca gagaataaga atattgtgcc cgttggttca
     2101 ctttggatcg acaagaaaga cgggaaagta atggaagaag atacgggtcg aacgttacat
     2161 ttgaaggatg cggcgtttat tgaagatccg cagcataatc aagtaatttt aaaaggaaaa
     2221 agttatggaa aagagatcca aacggcgtta ccttcagaag aatttgcgct aggaaatcca
     2281 ttccgatttt cacttgagac agtgtcagaa gtggcaaaac gaaaaaatga aatctattat
     2341 gaacaacaat tgtcacaaaa agaacgtgta taaaacaagt aaaaaagact atctgaaaaa
     2401 cggatagtct tttttctttt gggaggtttt aaaatgaccg agaaaaaaat gagtcttatt
     2461 gatcgatgta aacagatcga tatagtggat tttgctcgaa acaatggaat ggctgtagtc
     2521 aataaaggac gagattatcg cttagaagac catgattcat ttgtttttga tcgaagaaaa
     2581 caacgttttt attggaacag tcaaaatatt agtggcgaca ttattgaatt agctaaactt
     2641 tttttcatag acaaagagat ccaagaccct aagcaacaat ttaaagcagc cactgatttt
     2701 attctaaaga atgaagacaa aacagaacga gtggaaaatc ttcattttga aacagaaaag
     2761 tacaaagatc accccgttga ttatcaacca ctcacagaaa aaggaaggaa ttatctgaag
     2821 gaagaacgaa aactaccaga gtggctgatt gattatgcag aaaaagaagg gttgattgcc
     2881 gaactaaagc caaaatatga acggcaaaat ttcttagttc gagacgatcg tttagatcat
     2941 gcggtcgcct ttttatggaa agatccacag acaaaagaga cagtaggggc gagttatcaa
     3001 ggaacgttca ttgattatga acgttttggt gagagaggca catataagca tatagataaa
     3061 aattcgacag cgaatcatgg ctttaaccta aagatcggtg accctaagca gataaaattt
     3121 tttgagagta gtattgattt acttagctat gctgcactga atcgtgatca gttaaacgat
     3181 acgtggttag tctctatgga agggttaaaa caccatgtga tcagtcatta ttttggtgag
     3241 gcagtatctg aattgagaaa gaaacaagcc tttcctcaat ccatcgagat ttgtgtggat
     3301 aatgaccgtg caggacatat tttctacgaa aaagaacaat taatgggcgc agtcgatcca
     3361 tttacgaatc agaaagtacg ttgtgaacga ggaatcgcta atgattggca agtgccaaaa
     3421 gaatacaaaa tcatttatga agaagtcgca aaagaagaaa aggtcacacc agaagctatt
     3481 atggccatac acaagacaga aaacaatctg caattaacga accaactggt ttccgctcac
     3541 aaagtaaatg cgttttttgg ccagcagctt tctgttaacg attcaataga agccattaat
     3601 ttgaaagata tttgtagaga agtcgcaaaa gaattaaaag tttgtgaacg cgtggatggt
     3661 acgtacgatt ttgatcgttt ttatcaagaa aaaggggata ttaatgcgca gattcttttc
     3721 tcttataaag cagaacaata ctacaaaggc tataaaaatc atgaacatga atttgtacct
     3781 gaagtaaaaa aggactggaa cgatcaattg aagcatgaga tccagcaaca agaaatcaga
     3841 aagcaaaaac gtgccatgtt gtttcaacaa ggcagacaac aagaaaggga gtgaaatcgt
     3901 catgatgaaa accgttattt tagcagaaaa gccgtcacaa gcgaaagctt atgccgatag
     3961 cttctctaaa gccactcgaa aagacgggta ttttgaaatc caagatcgat tgtttcctgg
     4021 tgaaactgtg atcacgtatg gttttggtca tttagtcgaa ttagattcgc ccgatatgta
     4081 cgatgaaaat tggaaacaat ggtcattaga acatttgcct attttcccta cacattatca
     4141 ctatcatgtt cctaaagata agaaaaagca attcaaggtg gtgaaacagc aacttcaatc
     4201 ggccgacacc attattattg cgacagatag cgatcgtgaa ggggaactga tcgcttggac
     4261 gatcattcaa caagtaggcg ccgatcaagg aaaaaccttt aaaagacttt ggatcaactc
     4321 cttagaaaaa gaagccattt atcagggatt ccagcagtta cgagacgcaa aagagaccta
     4381 tccaaaattt gaagaagcac aagcacgtca gatcgccgat tggttaattg ggatgaacgg
     4441 tagcccactg tatagtttac tcttgcagca aaaaggcata ccaggaagct tttccttagg
     4501 cagagtccaa accccaacgc tttacatgat ttatcaattg caggaaaaga ttagaaattt
     4561 caagaaagaa ccttattttg aaggaaaatc cgaagtaatc gctcaaaatg gcgcatttga
     4621 tgcgaagctt gatccgaatg aaacccaagc gacgcaagaa gcatttgaag attatttaaa
     4681 agaaaagggg gttcaattgg gaaaacagcc aggcatgatt cgccaagttg aaaccgagaa
     4741 aaaaagcgcg gcaagtccac gtcttttttc tctatccagc cttcagtcta aaatgaatca
     4801 gctcatgaaa gctagtgcca aagacacttt ggaagccatg caaggcttgt atgaagggaa
     4861 atatctaagc tatccgcgaa cggatacccc ttacattacg gaaggggaat atgcgtatct
     4921 gttggatcac ttagacgaat acaagcattt tttgagggct gaagaaattt cgactcctat
     4981 tcatacaccg aatagtaggt acgtgaacaa caagaaagtc caggaacatt acgcaatcat
     5041 tcctacaaaa acagttatga cagaagcgac gtatgaaagt ctgacacctt tacaacaagc
     5101 gatttacgag caagttttaa aaacgacagt ggcgatgttt gcggaaaaat atacctatga
     5161 agaaacaacg atcttgacgc aagtccagca gcttcagtta aaggcaatcg gaaaagtacc
     5221 gatagatcta ggttggaaga agttatttgg caaagagagc gacggaaaag agaaggaaga
     5281 agagccgcta cttcctaaag tgacaaaagg agaaatggtg acggtagatc tacaagtcct
     5341 tgaaaaagaa accaagccac cacaacctta taccgaaggt acgttgatca ctgcgatgaa
     5401 aacagctggg aaaaccgtgg atagtgaaga agcgcaatcc atattgaaag aagtggaagg
     5461 aatcggtaca gaagcgacac gggcgaatat tattgaaacc ttgaaacaaa aggaatatat
     5521 caaggtagag aaaaataagc ttgtggtgac gaataaaggg gttttgcttt gccaagctgt
     5581 ggaaaaagag ccgctgctta cgagtgccga aatgaccgcc aaatgggaaa gctacttgtt
     5641 aaagattgga gaacgaaaag gaacacaaac cacttttcta gccaatatcc aaaagtttgt
     5701 ctctcactta ttggaagtcg tcccaggaca aatccaatcc attgattttg gatcgacgtt
     5761 acaagaagtg aaagcagcca gtgaaaagca agaagccgcg cgtcatttag gaatttgtcc
     5821 aaaatgccag gaacaagaag tcttactcta tcaaaaggtg gcagcttgta cgtctgaagc
     5881 ctgtgatttt aaattatgga caacgattgc caaaaagaaa ctcacagcga cacagctgaa
     5941 ggagattatt caaaatagcc gaacaagcca accagtgaaa gggttaaaag gtcagaaggg
     6001 ttcgtttgaa gcaacgattg ttttaaaaga agacttcact acaggctttg aattttctga
     6061 aaagaaaaaa acgaacttca agaaaagaac acgacgcacg acaaaataat ggagggttaa
     6121 tcttatgcgt aatacaacac acaaacaaca agaaatcgaa aatcaaaaag ccaaaattcg
     6181 ttcagaacaa gagaaattac gtgccagtaa aagaagactt cagcaattaa agaaccagca
     6241 acggattcaa aaagaaatcg aattagatcg atagacatta accttgttac aaatacacta
     6301 tcatgatata tttaatgtag tgtatttgta acaaaagggg ggaagtaaat gcaagagaaa
     6361 ctgaaagaat tgttggctat tcgcgatgga aatttatcta tgaaggaagc cagaaaatta
     6421 ggaatcgcag caaccactgt acaaagattg gtgaatcaag gaaaattgat caaagttgat
     6481 cgtggttatt atgtagagga tggtcatgga atagatgatt tattttggct tcagcaacgt
     6541 ttttccaggg gaatcttctc tcatgaaacg actttagata tttaccagct ttctacgaat
     6601 atgccgaaat tcattcactt aacttttccc catggatata acgttgatcg ttcaattaca
     6661 aaagagcagc agttacaatt tcatttcgtg aagaaagaag tttatgaact aggaaaagta
     6721 gagggaactt cctttcaagg aaatcctatt actatgtatg ataaagagcg tacactttgt
     6781 gatatttgga atccgagata tgaaattgaa tatgaaatga aattagaagc attaaaagag
     6841 tacatggaag acccattgag agatccaagt aaattaagag attacatgac aaaactacat
     6901 gtagaaaaag aaatgaaata ccacatgcaa agcttatatt gatggaggaa aaagatgaca
     6961 ccgacacaat ttgcagcaaa aacaagaaat ttagcaaaga ggacaggatt aaatgcacag
     7021 ttagtacaac ggcattttat gatggatcgt ttaattgaac aaatttctga atctaagtat
     7081 aaaaatgatt tcattttgaa aggtggcttt ttattaggat caaaatatgg aatcgataga
     7141 aggacgacaa ttgatatcga cacgacattt agaaatagca aattaactga agaaaaattg
     7201 aagagtattt ttaatgattt aactaggaaa cccacgaaag aaggtattgt tttctctatt
     7261 caaggaatga aggaaacaag agaagccgat ttttatcctg gatttcaaat taaattggtg
     7321 gctaatttag agaaagtacg tgtgccattt aaaatggacg tgacaacagg agattctatt
     7381 tatcctgaag tagatacaca ctatcaccaa ttaatgtttg aagataaaaa ggtggagatt
     7441 ccagcttatc caacagcaca aattattgca gaaaagctaa gtgctacgtt cagctttggc
     7501 acagataatt cgagagcaaa agattactac gacttattta ccattccgaa actagaaaat
     7561 atcgatacga aagagttata caaaagtgtt cgaaatacat ttacaaaacg tggggataca
     7621 aaaccgttgt ccttttatta tgaaagagaa atggataaga tcataggaaa tgactatttg
     7681 gaaaagcagt gggaaaaata tcgctcagtt aacacgtttg cgaattccat ttcatttaca
     7741 gatacagtca acgaaattga tcacctaatg aaaagtgtga tcaaaattga aaatcaagaa
     7801 agaaaacggc agtttaaaag atcacaacaa atgaatcaag aaatggagag ataaagatga
     7861 tcaataatgt aactttagta ggacgcttaa ccaaagatcc agatttacgt tataccgcga
     7921 gtggtactgc agtagcgact tttaccctag ctgtcaaccg aaatttcacg aatcaaaacg
     7981 gcaatcgaga agctgatttt attaactgtg tgatttggcg caaaccagca gaaacaatgg
     8041 tgacattagc taaaaaaggt agcttgattg gtgtagttgg gcggattcaa acgcggactt
     8101 atgacaacca gcaaggacaa cgtgtgtatg tgacagaagt tgtggccgat aatttccaac
     8161 tattagaaag caaagcagcg acagaaagcc gtgcgcacgc tgatcaatcg tccaccagtc
     8221 caagcaccac cacttttgaa caacgggata cagcgacgcc taataacaat ggattgaacg
     8281 ctttacagaa tccatttggg aatccatttg gtggacaatc aatcgatatt agcgatgatg
     8341 atctaccgtt ctaagtagca ttttctattg gttgcatttg ctacggttta tgttataatt
     8401 tagtcaacaa aaagaatttt caatctataa ccaaaaacca ctagctggcg ggctagtggt
     8461 tttcttttac gtttctatta taagaattaa cgggtaggct tactgtcttt cttggcaaat
     8521 ttacaatcaa gccatttgca gatatagtgc gcggctactt ctgccatgat tgcaaaaaca
     8581 aaatcaaaaa gaattttcaa acctataacc tcctcccctt gccagattag gccggggtag
     8641 cagacagcaa gactattata gcataattga aatatccaaa tgattggatt tttgctataa
     8701 ttttagcgag gtgattgtga tgaacaaata tgttggtttg cttgataaaa tccgagtaat
     8761 aaaaacgcaa ccgttattgg ttcgctttac tttacaaacg attcatgaat cgattaattg
     8821 tgtagttgca gatattgaaa ttatcgataa attgctaatt atggatgatg gaaaatacaa
     8881 tattgccgtc acaggtcact tcaataaacg aaatcagcta gttattgcta gcatgtatgt
     8941 tagaaatccc gatcatttta cgcgatcgat gggcatataa aaaaagaaga gttgcaatcc
     9001 taatgtagga ctgcagctct ttttgtttta catagaaaca agaaaaatga aaaggccttt
     9061 ttaggaaggc gcgagcgcct tctcaattga aaaacctctc tcactccaac cgccaccgcc
     9121 gttcgagaag aaaggaagtg aacgttgcac gttcactaag ataaagggtc ttgcaaagca
     9181 agacaaaata gaaaaaactt cctctcacca aaaggttcgt tcctgttcgt tatctcttta
     9241 ttgatttgcc agaccggcaa caagttgcct tttatcacca aaaagcgaca aggacaaggg
     9301 taaatggcac gctgcgcgcc cttgtccttg tcgcttttcg ttgattcatt ctggccaaca
     9361 aatcaataaa gaaagaacta acgaaaggaa gtaaaacctt tcctaagagg aggcagaatc
     9421 aatcatgcga aagaaatttg attattgggg tgttcccttc agcgaattat tcccaaacta
     9481 tcatgcacca catactgtcg aatgcgattg tggacaaaga gcgaaatgta tcaaaagcta
     9541 tcgtttgtat caatgtccaa catgcggaaa aaaatacacc ttgtcttatg gcgattatat
     9601 tttgattgat gagaaaggaa agaaacgctg atgaaaacat aacataaatt attgactgta
     9661 aaagaagtcg ttcaaatttt ggtggacaaa gaagtcattt ctgaaaaagt agcagaagct
     9721 ttacttattc cgcggtactg gacgatagaa acccctttag tagaggatgc accgactatg
     9781 ctatttgaag acctagtgaa gattgcagga tatgaactgc attatcaaga aaacatggaa
     9841 acttatgaaa acgaatattg gttaaccaaa ttagagaact aaaaaggaga aaaaaatatg
     9901 gctcaaaatt actatgacga atttgtcaaa ttacctttgg ataaaatggc acaaaaaatg
     9961 gaggatatga cattcctata ccatgagaca cgtgttccaa aaaaacatta taaggaaaga
    10021 ttgtcggtag ctgtggaaga aatgattgaa tcaggtgtcg aaatgaatct cattgcgacg
    10081 tactatcgaa cactggaaga actgaaaaaa caaaatgcaa aatggttttt ccaagcctta
    10141 ctatgcttgg aagtaggtgt caaaccaagc actattaaac cttctgaata tcaagcttta
    10201 gaactaacgt atgggaaatt tgtcgaaacg aaaaaagcca agactgtttc aagtgaatgg
    10261 ttagattatt ttgaaaatat caataagtat ggcgcctatt acaccatgaa aaaggaggac
    10321 aacgaaaatg aataaggtga tttatcaact aacaacggaa gatgtgtcac actttttaac
    10381 aaacaaagaa catttgaatg aagtaatcag acggattcaa aaagacttta gttgtatgga
    10441 agaaatgaag tactttattc aaagagtcga agaacaattg tatttggaaa gtgaaacgag
    10501 tcaaacgaag gtcacagtca gcaataatta tttagacgtg tataaacatc gtcctgaaac
    10561 ggtaaaaaaa ggaacggagc ttctacgact agaaacagaa aaatacatca tgaatgttta
    10621 cctagaaagg agtgaactgg tcgaaaactt tgtcagctgc acgatttgca ggaaacacta
    10681 ttactcagaa tcagaatatt attcaatttt tgagagcatg gaattaatgg aagaagaaat
    10741 ggaagagcta aacagcaaaa atgcgacgat agacgattat tatcttgtat ttactagatt
    10801 tttgacgtgt ggaacggaat ctatcgtgtt aatgggaatg gattatgaac tcgctgtacc
    10861 cgaagcattt ttgagggaat atgtagaaga agtagataac ccaggcgacg actggtacac
    10921 gactttaact gattttttag atacctacac ttacgacgat acagaagaaa tccaaaatga
    10981 acttgaccgt aaacgaatgc catatcaacg atttgaaagg aaaaaaaggc aataatcatt
    11041 tataccgaaa agtgagaatg tgtgaaataa gaacaaaaac cttgaaatta agggaatggt
    11101 tcttatttcg caccaatcgc tccaaccgcc cccgccgctt aggtggtgcg aaataagcgg
    11161 gcaaagcccg ataacctaag agaaagaagg agatccatat gaataaaatc attcctctgt
    11221 tgttcgtact gtgtattggc gagatactta tgcgtcatgg tgcagctgta catttaaact
    11281 tcgtagtatt cttaggaata ctagtatttt gtttgatttt ttggcgcttt gtgttagccc
    11341 ttgtgagtct actattcaag ttgattgcag ccatattttt catagggtta tttcgatgaa
    11401 aggagagagt actaatgcca attgcaatag gaaataaacg gttacccgtg acattagatg
    11461 aaaagagaca aaaagaattg cagcaactaa agcagaagta cggaaaaagt gaatctaaga
    11521 ttatgtgtat tgcgttagat ctattgatcg cgcaagaaaa agcaggattt gatataccag
    11581 cactaaaaaa gtgacgtcac ttttcatttg aagaaacaaa agtgatagca cttaattgtt
    11641 cctactattt agaatatttt ctatgtcctt tttattccat tttgtatcaa taactatgaa
    11701 atctttaaag tatccagatc aatttttttc ttacctgatt ctaaagattt tgttttctta
    11761 aacatattat gatttccttt cgactatttt catataaatg ttaacatagt ttatggattc
    11821 atggaatgtt aatctaattt aatagaaaag gataattaag tatatacgtt ttttcttatg
    11881 tatgttatac tatgtatata cagaaagagg tgtctactat ggaaatgtta cctataaaaa
    11941 aatggggaaa tagtaatggc cttagattgc caaagtatat tatggaatat cttggaattc
    12001 atacagaaga caaagtgaag ataatacaag aagaagtaaa cggtcagaag cggctaatta
    12061 tcgaagcggc taatatagaa aatgaattaa caattgaaca gttatttgaa aattacactg
    12121 atgaaagaaa tcatgtgacg attcaaaatt taggtgaagc agtaggtaat gaaaaatggt
    12181 gagaaaacct agacaaggcg atattctatt attgaataca gcacctcgat ctggacacga
    12241 acagacaggt aaaagacctt atattgtttt aagtcatgat attattgcgg attatagtaa
    12301 cgttgttaca gttgctccaa tttctagtac aactagaaac tatccgctat atgtaaatat
    12361 aaactcagaa tatgaaatga agacaacagg aaaagtatta ttagatcaac taacgacaat
    12421 tgattatgaa gccagagagt gtattttttt ggaaaaagcg catgatagtt tagttgagga
    12481 gttattgatg aaagttagaa cagtttttca aaaagtaagc aaataaaata gaaacagcag
    12541 catgatcaac tattcgtgat tgaccatgct gtcttttttt attctatata cggtttgtct
    12601 cgtaacattg tagtcttttg caatcttagc gatcgcaaca ccttttttta gatcctcaac
    12661 gatgttttta taaacaagac gacgttgggg atcttttgca ttggcactgt atagcttagg
    12721 acgtccttta taaacaccgt tcgcttttgc aatcttaatg ccttgtgcct gacgtcgttt
    12781 agattcggta cgttcttgtt cagcgatcat ggccaaaatt tgaatgatca aatctttaat
    12841 gaatttatcc aggagaggat tgccaattgc ttcggccatg atcggtagac ttgtgatgat
    12901 cagctgcaca tttttcttct tcaagtaatt gactgaagcg atgatctcgt cgtaatttct
    12961 tccaagtcga tcaatagctt ccacaataaa aatatcttga tctcggacga aatccagtgc
    13021 ttcttgaaaa acaggtcgat gagtaatcgt tgcgccagat tgtttctcaa tgaaaattct
    13081 ctctgcaccg aattttttca tttcttcaat ctgccgttgt tcattttgat cgatggatga
    13141 gacacgtatg tatgcaactc tcaaaatttt tacctacttt ctgcaactat ttaaaaaaca
    13201 ccctaatcat acacgagaaa accagatttt tcaatgtcgc ggtcgtgtct agttagggta
//
LOCUS       DAZBSA010000078        13086 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000078 DAZBSA010000000
VERSION     DAZBSA010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.078"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..2199)
                     /locus_tag="ACHGMT_002077"
     CDS             complement(79..2199)
                     /locus_tag="ACHGMT_002077"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFN6835807.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2429..3607
                     /locus_tag="ACHGMT_002078"
     CDS             2429..3607
                     /locus_tag="ACHGMT_002078"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFN6835808.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3623..4381
                     /gene="aroD"
                     /locus_tag="ACHGMT_002079"
     CDS             3623..4381
                     /gene="aroD"
                     /locus_tag="ACHGMT_002079"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFN6835809.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4404..4889
                     /gene="ybaK"
                     /locus_tag="ACHGMT_002080"
     CDS             4404..4889
                     /gene="ybaK"
                     /locus_tag="ACHGMT_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFN6835810.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4960..6213)
                     /locus_tag="ACHGMT_002081"
     CDS             complement(4960..6213)
                     /locus_tag="ACHGMT_002081"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFN6835811.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6329..7678
                     /gene="gdhA"
                     /locus_tag="ACHGMT_002082"
     CDS             6329..7678
                     /gene="gdhA"
                     /locus_tag="ACHGMT_002082"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFN6835812.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7790..9136
                     /locus_tag="ACHGMT_002083"
     CDS             7790..9136
                     /locus_tag="ACHGMT_002083"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFN6835813.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9444..9830)
                     /locus_tag="ACHGMT_002084"
     CDS             complement(9444..9830)
                     /locus_tag="ACHGMT_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFN6835814.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10098..10247)
                     /locus_tag="ACHGMT_002085"
     CDS             complement(10098..10247)
                     /locus_tag="ACHGMT_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835815.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            10709..10921
                     /locus_tag="ACHGMT_002086"
     CDS             10709..10921
                     /locus_tag="ACHGMT_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835816.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11199..12998)
                     /locus_tag="ACHGMT_002087"
     CDS             complement(11199..12998)
                     /locus_tag="ACHGMT_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFN6835817.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         4291 a         2354 c         2577 g         3864 t
ORIGIN      
        1 ttattctaag atttctcttt accaacatca tttgacaaag agcctaattt tgtcgctgct
       61 ccttttaaat ttattttatc attcggaaga acttgtggtc gttccgtgaa gttcttggta
      121 cgtttgcgtt ttataaagat caacagttga ttgtcccttg tgttcactaa agacactagt
      181 tgacttgtca ccaagagatt tttcagcttc ttttaatttc tctaagccat tcttgtagtc
      241 ataatcactg gtatcaattg ggtctaatcc actattcgta tagaaccgaa gaagatcacc
      301 attattgatt tggtcagaaa cagctaactg tttatcaact ttttctttca gtgaatcaat
      361 ttcattctgt acttcctcag taggttcagt aatagcttca ccagtttcgt tactatagat
      421 cgtaccaccg tagtaagtat attttggtgt aacaaaatca ccatcacgga aagaaacaag
      481 ctgttcatga tctttcgaga atagatcttg tcctaactga atataatttt ttgtatctac
      541 acctagtaag tgaagtaatg taggcaaggc atcaacttgt ccgccgtaag tgtgattgat
      601 tccgccctgt gtttgtccag ggatatggat catataaggg acacgctgca tttgtgcatt
      661 gtcgtagttt gtccaagtag aacttgtttt tccaactaat tcagctagat ttttatttct
      721 tgaattcgag acaccgtaat ggtcaccgta aaggacgatg actgaattat catacaagcc
      781 gctttctttc aaataattga agaattcttg tacggcggta tctaaataat tggctgtcgc
      841 aaagtaaccg ttgatcgttt catcatttgt attggctaac gggaatcctg cttcgtcatt
      901 ggtgaatttt gaataaggat aatggttaga tacagcaatg aattttgaat aaaatggttg
      961 ttgcagacgt tccaagtatt gtacggattg ttggaaaaat ggtttatcat gcaacccgta
     1021 ttggaacgaa ttgtcatcat taacatcata ataagaagaa tcaaagaaat aatcatatcc
     1081 taaattcttg tatgtctcgt ttcgattcca gaatgtacca gcgttcccat ggaataccgc
     1141 actagtgtaa ccttgtgttt gacttaaaat atcaggtgca gcttggaaag tattttttcc
     1201 gcctaattgt gtgaacaagg agccttgatt caagccaaac aatgaatttt ccatcagcgt
     1261 ttccgcatcg cttgtttttc ctgcttttac ttgatggaaa aagttgtcaa aactgaacgt
     1321 gctgttgctg tggtaaagac tattgatgaa aggtgtaact tcaagctgga gaccattctc
     1381 atctgtcaat ttataatcga tcaggaattg ctggaagctt tctaaatgga tataaatcac
     1441 attttttcct ttagcgagcc cgaacaaatc atcatttggt gctgcataat gttcttttac
     1501 ataattttct acttctttca tatcattggg acttgcttct gcccgcactt ggtttgtctg
     1561 gtaagtttga acaccgtcat aaaccatgaa agcattcaag ccaagatatt tgaccaaata
     1621 atcacgagag aatgttcgag tcagcaattc tggacgatca gcttccgcca taaaaagatt
     1681 tcctgagaaa atcataactg ctaatgtaga gatcgcaaaa gccattcttg ctcggatcgg
     1741 gcgagggtcc aacttgattt tttttgcaat cagcagaaca gtcaagataa taaaatcgat
     1801 ccaatagatc acatcatatg ggcgaaacag tcgaatggca ctttctccca gaccactcgc
     1861 tactttccca gcacctaaaa tcgtgttgat cgtaatgaag tccgtgaatt ctcgataata
     1921 agtgacattc gaaaaaacaa gcaatgtcaa aagaaaataa atgaccatca tcgtgatata
     1981 agcaactcta gggcgtctga tataaagagc aattccaagt aataacaatg tagttgcaat
     2041 agggttgata aacaagataa agtattgaat gccactttcg attccgagat ggaaatcaac
     2101 taaataggca aaaatcgttt tcaaccaaag taaaacaact agtaaagaaa aaaatccgag
     2161 cctcttattc aaaaaggcag gtgtttttat acatttcaag ataatcttcc tttctgcact
     2221 tcttaatcac agaagtgtca tgaaaccatg ttacacagct cacttattct catttatttt
     2281 actagtatga caatatagtg tcaattgaaa ccagaagttt atgctgtttc attaaggttt
     2341 tctttaagct tcatgaaaaa atgagcagta agacaagtcc tatgtttttt gttatacttg
     2401 ggaagagaaa gctaaagcag gtgaaggaat gaaagtaaaa gtaaaaaaaa gagcaagcaa
     2461 tcgattgaaa aaaggatatc cactgatcca aaaagaagac cttgctcatc cccaaaaaga
     2521 aaccacgcaa tgggtggaat tagttgattc gcaaaaccaa tttatcgcaa aaggatatct
     2581 aggaaagcag aataagggaa tcggttgggt attgagccag cagaacatcc catttgatca
     2641 atcttttttt gaagaatttt ttgtgaagtc aaaagaaaaa agaactttct ttttttcaga
     2701 tgattctact accgcttttc gtatattcaa tggagaagga gacgggatcg gcggtctgat
     2761 tgttgattat tatgcaggtt atgctgtttt ttcttggtat aacgaaacgt tatatgcaca
     2821 tcgtgaatta ttgattcaag cttttcgtga aatctacccg gaaatcgtag gaggctatga
     2881 gaaaattcga tttgaatcag taaatctgcc tgagagccag ttcatttacg gaaaagaggc
     2941 accggaaccc ttgatcgtaa aagaaaacgg agtaaaattt gccgtttatt taaatgaagg
     3001 attaatgact gggatctttt tagaccaaaa agaagtacgg ggatcattag cagaaggttt
     3061 cgcttctggg aaaacagttt tgaacatgtt tagttatacc ggcgcctttt ctgttgctgc
     3121 cgcaatggga ggggcaatcg aaacaacaag tgtcgatcta gcaaaacgca gtttaaaaaa
     3181 aacacaggaa cagtttgaag tgaatggttt ggatgtcgat cagcaaaaaa tcattgttat
     3241 ggacgtattc gagtatttca aatacgccaa aagaaaacaa tttttatatg acatgattgt
     3301 attggatcca ccaagttttg ccagaaataa gaaaaaagtc tttcgcgtag caaaagatta
     3361 tggggaactg gtcaaggata gtctggatat tttagcagaa gacgggatac ttattgcgtc
     3421 tacaaatgct gcgaatgtct cgatagaaca attccaaaca atgattgaag aggaattcca
     3481 tgccaaaaat gtttcttttg aacaacttgc tttatatcgt ctgccaaaag atttccagac
     3541 gacggatacc tttttagaag gcaattatct gaaagtgttt gtttatcaaa ttaaaaataa
     3601 ccattagaag gagcgatgca gaatggtttg ggtaaaggga ttgaatatag gaagcggtag
     3661 accaaagatt tgtgcgcctc ttatcggggc cgtccgagag gaagtattac gtgaagcagt
     3721 tcttgcacgt gaagcagggg ccgatttagt cgaatggcga gtcgatcatt atcgtgaagt
     3781 tttttctttt gatgaagtag cagaaacatt ggttcttatc catgagatgc ttgatggact
     3841 gccgttatta tttacgtttc gtaccttagc tgaaggcgga gaacaagaca tatcgatccg
     3901 tcagtatcgc gacctttatg aacatgtgat acacacagga ttagtagata tggtcgatat
     3961 cgagttgttc aaagtagaaa gtctaggaaa aagactgata gaggaaatca agtctttaaa
     4021 cattccttta attatcagta gtcatgattt taaagaaaca ccagcagacc ctgtcttgct
     4081 ttaccgattg aatgttatgg aacattttgg agccgatatc ggaaaactgg cagtaactcc
     4141 caataatgaa cgagatgttt tgcggttgat ggagttgact agacgggcaa atgcttttgt
     4201 ctccatgcct attattacga tgtccatggg aaatcttggt aaaatttctc gtctggctgg
     4261 cggaacgacg ggttccgtta tgacatttgg ttccttaagc agcgattcta cttctgctcc
     4321 aggacaatta ccggtcaaag cattgaagca aattattcaa ttattaaatg aaccgttgta
     4381 gaacaagttt gaggtgatat tttttggcaa agaaaaaaat agtaaaaacg aatgcaatcc
     4441 gtatggtcga acaaaaaaag attccctata cagagcacga atatgagtgg gacgaaagcc
     4501 acctaagtgc gtcatcggtc gctgaacagc ttccagaaag ccaatctcat atatttaaaa
     4561 cactggttgc tgtgggcaat gtgacggggc ctcttgttgc tgtgataccg ggagaagccg
     4621 aattgaatct aaaaaaatta gcaaaagttt cagggaataa aaaagtagaa atgctccatt
     4681 taaaagattt ggaagcaacg accggataca tacgtggtgg ctgctcgcct ataggtatga
     4741 aaaaactatt tcctacttat ttagatcaaa ttgcagaaag ctatgaacaa atcattgtct
     4801 cggctggcag aagagggcta cagatggagt tagcacctca agatatctgt gcattaacga
     4861 gcggacaatt tgcagatata aaacaataaa tcatacgtat aaaagaaaga aggaggccgg
     4921 gatgttgttt gtccagtctc cttctttctt ttttatacat tatgctcttt gcccggcatt
     4981 cattcctgct acatgccctg tagcgaaagc cgctgtgata ttaaagccgc cagtataacc
     5041 attgacatcc agtaattctc ccgcaaaata cagaccatca atgattttac tctccaatgt
     5101 tttaggataa atttccttta gagaaacccc tccgccagtt acaaatgatt tctcaatagg
     5161 aaaagttttg ttgattgata gcttaaactc tttgcatagt tgaacaaaat cttgaatttg
     5221 tttttgagag gcttgttgtc ctgtcagatg atccatttca agccgctcaa gaaagaaaac
     5281 taacaatcgc tcaggcaata taccatgcca agcattgacc aagttctttt tggttgcttt
     5341 acttttttct gtcaatatat gaataagctc ttgtttggtt tgtgtaggga aacaatccag
     5401 agaaagtgtc actggttctc ccgttttctc taattcttta ttgacaaaac ttgaacaacg
     5461 cagtgcagcc ggaccagaaa taccgaaatg ggtgaaaagt aaatccatcg tatgttcagt
     5521 caatacacga ccttttttat ttaaaacgcg taaagtgatg tcttgtaaag aaagcccttg
     5581 aagtgttttc tcctgaatat acggttcatc cgatatcaag ggggactctg tcgcataaag
     5641 aggcgtcacc gtgtgaccca ctcgcttcac gatcttgtac ccatctccag tagaaccagt
     5701 agatggataa gtccgtccac cagttgttaa gataatggca ggagcatgga actcttcgaa
     5761 ttccgtgcgt ataccgtaaa ttttatgttc tttgtggatc aatttctcta ctcgagtaga
     5821 gaatagaagc gtcacatcta gttctttcag acgattgagc aatgcttcga taatcgtttt
     5881 tgatttattt gttacaggaa acatccgtcc atgatcctct tcttttaaat gtacgccttg
     5941 tgattcaaag aagttcatga tatcgaaatt attccattgc gcaaacgtac tatataaaaa
     6001 ctttccattt ccaggaatat gtgcaatcaa atcatcaaca ggacgattgt tcgtaacatt
     6061 gcaccgacca ccacctgtca tcagtagttt ttttcctgct tttttgtttt tttcgatcaa
     6121 gagtacgcgt gcgccttgtt cagctgcact gatcgctgcc atcatcccgc ttgttccagc
     6181 gccgactacg atcacatcat acagtttttc cattccctca cctcaaggaa agtgtagcat
     6241 atctgaagaa agaaaacaga aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga
     6301 gggaagataa gcaaaggagc gtggaacgat gacaaacgca acagaatatg ttcaagcaat
     6361 tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa tttttacaag caatcgatga
     6421 attcatgccc acggttatgt cttttttgga cactcatccc gaatatatcg aaaagaatat
     6481 cttagaactt ttaacggaac cagaaagagt tattcaattt cgagtgcctt ggcaagatga
     6541 tgaaggaaac tggcgagtaa atagagggta ccgtatccaa tacaactcag ctatcggccc
     6601 ttataaaggt ggattgcgct tccatcctag tgtcaatcta agtatcttga aatttttggc
     6661 ttttgaacag attttcaaga atagtttaac cggtctgcca atcggtggag gaaaaggcgg
     6721 aagtgacttc gatcctaaag gaaaatctga taatgagatc atgcgttttt gtcaaagttt
     6781 catgctggaa ttgtcaaaac atatcggccc atcgattgat gttcctgcgg gagatatcgg
     6841 tgtaggagct agagaaatcg gctatttgtt cggcgaatat aaacgattga aaaaatatga
     6901 tactggtgtg ttgacaggta aacctttgga tttttgggga agtaagatac gtacggaagc
     6961 tactggttat ggattagtgt attatgtcaa acatctattg aacgaagaaa aagattcatt
     7021 taatcaaaaa acagtttttg tttccggaag cgggaatgta gccatttatg caatcgaaaa
     7081 agtgcaagag ctaggcggga aagttgttac atgttcagat tctagcgggt acatctatga
     7141 tccagaagga atagatgtga aactattgaa agaagtcaaa gaaatcaata gacaacgttt
     7201 gacggaatac gcaaaccaac gtccttctgc tatttatcat gctggagaat ctgtttggac
     7261 attgaaagaa aaagcggata tcgctttgcc atgtgctact caaaatgaaa tagacgaaag
     7321 cttggccgaa atcttagtag aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc
     7381 atgtacagta aaagctgttt ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa
     7441 agctgctaat gctggaggag tagcagtatc tgctttagaa atgagtcaga acgcccagcg
     7501 attgacttgg gaaagagagc aagtggatga gcagctggat caaatcatgg aaacgattta
     7561 ccatacgtgt cgccaaacag cagccgagta cggcgacgaa aaaaatctgc ttcttggagc
     7621 aaatgttgct ggatttgaaa aagttgccaa agcaatggca gcagaaggtt tagtttgaaa
     7681 aatgaaactt tcttggaaat attgtcagca ttttgtttat ttgcttgaaa aataataaaa
     7741 aaaaacgtaa agtattacct gtagagtaaa cctataagga ggagaacaaa tggcacacat
     7801 tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa caagagttag ggtacatgca
     7861 aagccaagta acagcagccc acaacgaatt acgggaaggt actggcgcag gaagcgactt
     7921 ccgcggctgg atcgatttac caacgaacta tgacaaagaa gaatttgcac gtatcaaaga
     7981 agcagctaaa aaaatccaat ctgattctga agtattagtt gttatcggta tcggtggttc
     8041 ttatttaggc gctcgtgcag cgatcgactt cttgaatcac acattcttca atttattaga
     8101 tagcaacaca agaaaagcac cacaaatctt ctttgccgga aattcaatca gctcaacata
     8161 tattgctgac ttgatcgaag taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa
     8221 atctggaaca actactgaac cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa
     8281 gaaatatgga caagaagaag caaacaaacg tatctacgct acaacagata aagcaaaagg
     8341 tgcagttaaa gtagaagcag atgcagaagg ttgggaaact ttcgtgattc ctgatgacat
     8401 cggcggacgt ttctctgtat taactgctgt tggtttgtta ccaatcgctg ttagtggtgc
     8461 agatatcgat gcattgatgc aaggtgccgc tgatgcaagc aaagcatact caagcgacaa
     8521 actagaagaa aacgaagctt accaatacgc agcaatgcgc aacattcttt accgtaaagg
     8581 caaagtaact gagttgttga tcaactatga accaggaatg caatacttct cagaatggtg
     8641 gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc
     8701 aaacttctca actgatttgc actctttagg acaattcatc caagaaggac gtcgcaatat
     8761 ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc
     8821 agaagattta gacggtctag gctacttgca aggaaaagaa gtggacttcg taaatacaaa
     8881 agcatttgaa ggaacattgc ttgctcatac agacggtgac gtgcctaact tgctagtcaa
     8941 aattccagaa atggatgctt atacattggg ttatacaatg tacttctttg aaattgctgt
     9001 aggtatttca ggttacttga atggaatcaa cccattcgac caaccaggtg tagaagctta
     9061 caagaaaaac atgttcgcac ttctaggcaa accgggcttt gaagatcttg ctaaagaatt
     9121 gaacgaacgc ctgtaagata agggtttttc gctagattta aataatcagt gatagaaaca
     9181 tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact caaacatact gcatcgttta
     9241 ttcagaataa ggggctgtga caagacgttt gtcacagtcc cttgtttgta tagaaatcga
     9301 ttgattgcct tgctctttag tttgttttag gactttaaga acagtgaggc tgacttcgaa
     9361 ggttaattct gtttttttgt tttctagaat cagtaagcaa aaaagcactc acttcgagtg
     9421 cttttttgcg atatattctt agcttaagat aggcctaatt tttcttttac agatgagggt
     9481 accttatcgc tggtaattac ttttttctct atgatccgct tttcgcttgc tttcaactgc
     9541 aagatcgttc cttcggtgta agcagaacca ttggcagagt caccagaagt aatgacatcg
     9601 aaatctaact gtcttttttc accgttttca gcataagcat ctacgttgta tttaaaacct
     9661 cttccaatta cttccccatt atctgctttg atatcagttt ctgcaggcat tggagctttg
     9721 actaccgcat aataatcttt acctacataa gtagactgat agtagtccca acctttccat
     9781 cctataaaag caatcaatat gataacgatc aaccctaaaa tttttttcat gaaagatctc
     9841 cttttttatt tttattatat taaatattat acagataata acggaatatt atcatcgaaa
     9901 cagctaacaa aattgtcata aaaaacgaga tggttgatat tagaacttgt gtttttgttt
     9961 aggtctagag ataaagagag acaatttcca aatatatgta atgaatacaa aaagacgttt
    10021 gtcgcggatt accgttacaa acgtctttta ttgattttcc gagatctcat cagtcgccat
    10081 ttggaatggt tcactcctta tctagattcg tttaatgctc ggactgcttg ttcgattgta
    10141 ataccaaacg caacgttatt ttcgttttgt gcatcaatcg ccataaaacg gttagaagtt
    10201 gtatcaaaag ctacgcggta ttgcatattt gtttttgtaa acgtcataaa attcatctcc
    10261 tttgtaatgt aatttagttc ttgaaagttt cctttaaact ttcgaactta taccaagttt
    10321 agcatcgtac gttttttgat gcaattaaaa agcatttatc attttcctta tttatttttt
    10381 cttaaatggt ttagctattt tcttttaatc cgataagtag cccaatcatc aaggttacaa
    10441 atactattgc ttgaatataa atcatatgct cacctcttta taaataactt agcaatataa
    10501 agaaaaaaac gcacgaacaa cataggaata taaacaaacg tttttgtcac aacgtctaag
    10561 atcgtgttaa aaaatagtta tctaaaaagc agaaagatac tcaaaataaa aaataaaaaa
    10621 atgaataaaa acgttttcaa atacaaggta atttgttatt ataaaataaa gaaaaaacaa
    10681 aataaaaata aagaaaagag ggaatgaaat ggtaaaagca gaagtaggaa gatcttatat
    10741 atgcaaaccg atcggagtat ctcatgaact aatcggctgt attgaacgta catatatcaa
    10801 cacggctgtc gtgtatgttg aaagttatga ccaacgagac tatcaactta tcaatgaaag
    10861 caagtatcgc atgctcgtaa aattctgtga tattagcgca cctactgaat tggtttctta
    10921 aacagtattt ttgagaattc gcaagaactc ccttataata taaataaaga cagaagggag
    10981 cgagaagata tgccaaaaag aaaaagttca gggaaaatgg gaattattct tgcagtgatc
    11041 tctgcaattc ctgctatttt agctattgtt cgtgcgatga aggaatcaag agaaagttaa
    11101 gcacacatta tgagcgtcaa acaaaaaacg catatacctg cggtggcagg ttatgcgttt
    11161 tttgttgtat aaagactttt ttcattaacg atttttgatt agccgactcc caaaacaaaa
    11221 tttagcagtt tatctgaata agtgatttct tcatctgttt gtttcacact ttcatttagt
    11281 agtcccatct catcggttat gataccatcc acaccataaa acatcatgcg actcatggca
    11341 tctgaatcgt tgattgtcca tacatatact tcttttccgt cttggtgggc agaattaatg
    11401 aagttacggt tgatcgtact atattccatt gtcagaaaat cagcaggcgt cacagggggc
    11461 ccaacaatat tgaaaggtaa gatgtagccc acataaaagt ttggttctgc ttcttttaaa
    11521 tctgaaacgg tttggtacgt caaactttgc aaaatatgat ggttctctaa gataactttg
    11581 cggtatttat ggacaaatcg ttcgacaagg tcatccgaat cttttttaga ggtcttgatc
    11641 tcaataagta atttttggtt tagcttatgt gctttttcca aataagaatc aaaagaacaa
    11701 acaggggctt gcactccgtt ttcagtaact gtaagatttg tcaattcttt caatgtaaga
    11761 tcctgtggtg ttccatttac ccctgttaag tgacgaagat ttgcatcatg catcactacg
    11821 aaatgaccat cttttgtttc ctgaacatcc atctctacat aatcaggatg atagtctctg
    11881 cttgtttttt caagtgcttc taaagtattt tgcgccccat tttgggtact cacacctctg
    11941 tgagagacag tcactggttt tgtctgaaca gctgaagtca gataatccat attgtaaaga
    12001 cagacgccaa taccgaaaaa aactgcaaaa agagtcaaac cagtgttttt cagcgcagag
    12061 aaacgcaagt tgggggcttg tgggataaac cacttaggga tctcaggcaa gaagccttcg
    12121 tcatccatga aatcaacaat gatataaaaa atccctacgg tagacatcac gatattgaat
    12181 aataaaatcc cttgtaaaag cgtcatagca aacacagcgc tgatcaatgc atagtctggg
    12241 aaaaattgtt caaccatact ttgtgccaaa ataacaacga tatatcctaa agaagaaagg
    12301 agtaaaatcg taccgccaat cacaataaat tggcccgtga tggccaaaag acgcgatttg
    12361 gtcaatgacc agctttcccg aatggctgct cgaaaagaac gatctcgtag aatcatttcc
    12421 ggtaaagcaa aaatcagtcg aatcccaata tagccaagga aaatataaac gagtaaaaag
    12481 gaactgacga tgatccaacg attagcaaaa ataaaatcca taatgaaagc ggggattttt
    12541 atttttgaca gtagatcaga attaaaactt aatccgctga tcggtagaat caataaaaaa
    12601 taagcaagga aaaacaaaaa agtaatgggt ctcactttct tcagctgaag tatcgtcaag
    12661 tgtaagagtt gcttcaaaga gattggttct ttctttttaa taaaaaaaac actcatcaaa
    12721 agaaaagtaa attcgaaata aactaacagt aaaatcgtca tcaaaactac gattaacgag
    12781 agtagaaccc caggatgttg agaaaaaatt gaaggaatat tgtcataaga caaatagtca
    12841 attgcccctc ggtgtaaaat aaaacgggtc gtactgctca ataagggtaa acaaataaat
    12901 aaaataaagc catgcatcag caagacatca cggaaataag cttgtgttcc ttttaaaaag
    12961 tcccaagcat tacctaagct acgtttcaaa taattcatcc tatcacctgc tcttcacatt
    13021 gtagcgcaaa tctaatgaaa aaggaaaggg tgcagtgtgt tttgcagacc ctttcccaga
    13081 tttaac
//
LOCUS       DAZBSA010000079        13086 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000079 DAZBSA010000000
VERSION     DAZBSA010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.079"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(451..1761)
                     /locus_tag="ACHGMT_002088"
     CDS             complement(451..1761)
                     /locus_tag="ACHGMT_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFN6835818.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="ACHGMT_002089"
     CDS             complement(1773..3191)
                     /locus_tag="ACHGMT_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN6835819.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3431..4999)
                     /locus_tag="ACHGMT_002090"
     CDS             complement(3431..4999)
                     /locus_tag="ACHGMT_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFN6835820.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5003..5413)
                     /locus_tag="ACHGMT_002091"
     CDS             complement(5003..5413)
                     /locus_tag="ACHGMT_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFN6835821.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5410..6333)
                     /locus_tag="ACHGMT_002092"
     CDS             complement(5410..6333)
                     /locus_tag="ACHGMT_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFN6835822.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6330..7208)
                     /locus_tag="ACHGMT_002093"
     CDS             complement(6330..7208)
                     /locus_tag="ACHGMT_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFN6835823.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7195..8532)
                     /locus_tag="ACHGMT_002094"
     CDS             complement(7195..8532)
                     /locus_tag="ACHGMT_002094"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFN6835824.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8535..9638)
                     /locus_tag="ACHGMT_002095"
     CDS             complement(8535..9638)
                     /locus_tag="ACHGMT_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFN6835825.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9712..11220)
                     /locus_tag="ACHGMT_002096"
     CDS             complement(9712..11220)
                     /locus_tag="ACHGMT_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFN6835826.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11186..11878)
                     /locus_tag="ACHGMT_002097"
     CDS             complement(11186..11878)
                     /locus_tag="ACHGMT_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFN6835827.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11900..12874)
                     /locus_tag="ACHGMT_002098"
     CDS             complement(11900..12874)
                     /locus_tag="ACHGMT_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6835828.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
BASE COUNT         3889 a         2798 c         2117 g         4282 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta
     3421 aagaattaaa ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac
     3481 ttcctctttt tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc
     3541 ctgagagtat acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt
     3601 ctttcaaatg aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt
     3661 ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac
     3721 gtaggcattt tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat
     3781 ttaagtacag taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg
     3841 tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag
     3901 attgaagaga ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc
     3961 aatcagcatt tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg
     4021 tatccataaa ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt
     4081 cttccatcgg agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca
     4141 cttcttgaat agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa
     4201 gatatgtcat gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt
     4261 gcaatgtaga agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt
     4321 aaggaccagt aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc
     4381 cttctgccgt catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt
     4441 tcgctaattc tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg
     4501 catcatattc ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc
     4561 atagttcatt gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa
     4621 agccgttact atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga
     4681 tcaaataatt gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa
     4741 gataaaacaa tccatcagca taactgattt gtggcgccca tatacttcca tttgtaggat
     4801 ttccttgcaa agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat
     4861 ttttcgactc atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat
     4921 accgctcttt cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt
     4981 tcactttgat agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga
     5041 atgccccagc tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca
     5101 acaatatgaa tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag
     5161 cagtcaaaaa caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca
     5221 gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta
     5281 cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg
     5341 ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc
     5401 attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat
     5461 cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc
     5521 atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa
     5581 gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc
     5641 agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc
     5701 tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc
     5761 atctgcccca ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg
     5821 acgacaatac tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa
     5881 atcaggcaac ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa
     5941 agatacgcga tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc
     6001 tgctgtattt tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt
     6061 agcacttcgg aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt
     6121 accggagata actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat
     6181 atattcatca tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc
     6241 tgccaaataa tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt
     6301 ctttccaatt atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa
     6361 atatttctca tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt
     6421 tctggactac ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct
     6481 tcgtccaagc acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt
     6541 ttttcttgga ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac
     6601 ccatgatgat ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga
     6661 tcaatacaaa ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc
     6721 atagaaagac caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa
     6781 tcgatcaagc tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa
     6841 aattgtttga acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca
     6901 atcgaccaaa cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag
     6961 taaggagcac catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc
     7021 aggtaaagga ccggcggaaa cggtccttct ttttctggat aagcatagag ggctggtact
     7081 gtttccaatt catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct
     7141 cggtataatc gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc
     7201 aactgcataa gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga
     7261 ttggaatttc tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg
     7321 attttcaatc gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg
     7381 tctgctcatt tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc
     7441 catcgagaca tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata
     7501 aataaaacct gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct
     7561 gaatgctggt gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt
     7621 ccctcgcgga ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat
     7681 gactatattg gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa
     7741 tcgtattcct cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact
     7801 accactcatt tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt
     7861 gatcgtgata ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc
     7921 aatacagtca tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc
     7981 tggatcaatt ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa
     8041 cgttgcatta ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat
     8101 atctttcaca gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc
     8161 gagattatct ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt
     8221 accgaaacca gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac
     8281 ttttcgatgg actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga
     8341 gaatttcaga accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc
     8401 tcctgttaga aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc
     8461 atcaatggct tgttgtattg cttcagtatt caattcatca attgatgcgc caaatgtcaa
     8521 aatatcatac atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg
     8581 cctttttgat cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc
     8641 tgatctgctc cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct
     8701 tttgtttcca gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc
     8761 cattcttctt tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata
     8821 ctactgccgg aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac
     8881 catgtcccgc tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt
     8941 tctttcagca aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc
     9001 atcaaatacc agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca
     9061 gcccatggtt ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg
     9121 aatagatgtt tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca
     9181 ctaaaattct ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa
     9241 atttgataag gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt
     9301 gccaactgct gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg
     9361 tacaataaaa agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg
     9421 tagccacgga tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca
     9481 aaataaattt tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca
     9541 tagtcataag accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc
     9601 atgatacttt ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt
     9661 aaaataggat ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc
     9721 cgctgctttg tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca
     9781 gtcttctaca gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt
     9841 agcatcaatg acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa
     9901 tggcacagaa ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa
     9961 ttctttttca gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga
    10021 taacggttgc acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc
    10081 attcaattca taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc
    10141 taaaagatca tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga
    10201 tttgggaaat gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc
    10261 gccttcacca tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg
    10321 acccatcgtc ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg
    10381 tgcaaattgt tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc
    10441 attctcaaat agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc
    10501 tggagtgaat ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt
    10561 gaaaagttta aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt
    10621 gtcgtccaca ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag
    10681 ctcatccatg gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac
    10741 taagccagca cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact
    10801 cgcagcttcg attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc
    10861 tgatacatcc cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat
    10921 cgcaatttga agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct
    10981 ttcttctttt gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc
    11041 gatcaagttc acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt
    11101 gattttagct tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc
    11161 ggcaagtgct agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat
    11221 aaattttctc cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg
    11281 agttcttcca ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga
    11341 agagactgcc accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa
    11401 tccctaccat acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa
    11461 atgccatttt cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa
    11521 tatagagtgc tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc
    11581 aattgtgtat gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc
    11641 tcatcagcaa ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag
    11701 gaaacgtttc ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag
    11761 gaccgaaagt aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat
    11821 aaaccaactg taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat
    11881 ctttccctct ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat
    11941 tagctaaaat taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga
    12001 aacttaactg tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa
    12061 cgcttcgatt caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct
    12121 gaccgatttt tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat
    12181 gccatgtttt gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat
    12241 cgactgcagt tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca
    12301 cttgtccgat atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa
    12361 atcccattgt tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag
    12421 taatcgaaac aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc
    12481 cgtttttcaa tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg
    12541 gaaaaaacac gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga
    12601 atgtcggttc tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta
    12661 ttcctaaaaa tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc
    12721 taaaaatcaa gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt
    12781 tgattcctgc aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca
    12841 gttgaaatgg ttcttgtttc tttacacttt ccataagccc ctccttagtt ttgtgaatgt
    12901 ttgctgctta tgacttcatg atagataact atctgaatta cgtatataat catttttacc
    12961 gtaagtaaaa acgcttacat agcaatgatt tttgtgctat acaaaacgat aaagtgtatt
    13021 ttttcacaaa taaactacgc atagcaaacc ttctattttt ttctatctga cacttagcat
    13081 aggaaa
//
LOCUS       DAZBSA010000080        13051 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000080 DAZBSA010000000
VERSION     DAZBSA010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.080"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            152..1075
                     /locus_tag="ACHGMT_002099"
     CDS             152..1075
                     /locus_tag="ACHGMT_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFN6835829.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            1081..2334
                     /locus_tag="ACHGMT_002100"
     CDS             1081..2334
                     /locus_tag="ACHGMT_002100"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFN6835830.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            2468..3814
                     /gene="gor"
                     /locus_tag="ACHGMT_002101"
     CDS             2468..3814
                     /gene="gor"
                     /locus_tag="ACHGMT_002101"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFN6835831.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGIGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(4628..5581)
                     /gene="menA"
                     /locus_tag="ACHGMT_002102"
     CDS             complement(4628..5581)
                     /gene="menA"
                     /locus_tag="ACHGMT_002102"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFN6835832.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(5594..6697)
                     /locus_tag="ACHGMT_002103"
     CDS             complement(5594..6697)
                     /locus_tag="ACHGMT_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFN6835833.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(6787..7722)
                     /gene="pplA"
                     /locus_tag="ACHGMT_002104"
     CDS             complement(6787..7722)
                     /gene="pplA"
                     /locus_tag="ACHGMT_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFN6835834.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            8024..9958
                     /locus_tag="ACHGMT_002105"
     CDS             8024..9958
                     /locus_tag="ACHGMT_002105"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFN6835835.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            10415..10831
                     /locus_tag="ACHGMT_002106"
     CDS             10415..10831
                     /locus_tag="ACHGMT_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFN6835836.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            10845..11411
                     /locus_tag="ACHGMT_002107"
     CDS             10845..11411
                     /locus_tag="ACHGMT_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFN6835837.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            11434..12420
                     /locus_tag="ACHGMT_002108"
     CDS             11434..12420
                     /locus_tag="ACHGMT_002108"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFN6835838.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(12575..>13051)
                     /gene="scm"
                     /locus_tag="ACHGMT_002109"
     CDS             complement(12575..>13051)
                     /gene="scm"
                     /locus_tag="ACHGMT_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFN6835839.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         4065 a         2369 c         2695 g         3922 t
ORIGIN      
        1 tcacaatctc tttttatatt tggacagaat atatcgaact ttattttgat tttttgtgaa
       61 ataattttca aaaatccgac tttaaataga agaagtattg gaaagttttt agagctatgc
      121 tactattatg aagttgtagg aggagaaaat tatgatcgaa ctcataacca cagtagaaac
      181 tattgaacaa gcagaacagt tgcttgctgc aggtgcagat acactatatt tcggtgaaga
      241 gcaatttgga ttgcgtctgc ctgcttcatt tacacgagaa gaacagcgac aattggtaga
      301 attagcacac aagtatggta aaaaagcgaa tattgctgta aatgggatca ttcatcctga
      361 aaaaatgaaa aatgtacctg aatatttggc ttttttaaaa gaaataaacg tagatgtgat
      421 cacagttggt gatacaggta ttatttatac gatgaaaaaa aatcctgaat tgcgtatacc
      481 gtatatttat gatgctgaaa cattagtaac gagcgcacgt caaatcaatt tctgggcgaa
      541 aaaaggcgca gttggagcag tacttgctcg tgaagttcct tttgaagaga tgaaggcgat
      601 ggaagaaaaa ctcgatattc ctgtagaaac ccttgtgtat ggtgctacat gcatccatca
      661 atccaaacgt ccactgctac agaattacta taactatacg aaacaagacg aacaaaaaga
      721 tcgtgaaaga ggtcttttta tctcggaacc taaaaaagaa gagacacatt attctattta
      781 tgaagatagt catggcacac atatttttgc cagcaatgat ctgaatctct cgaatgaatt
      841 gaatgaactt tatcagcatc attactgtac atggaaattg gacggattat ttactccagg
      901 cgaaaaattc gtaaacatca ctcgactatt cgccgaagca aaaaaacaaa ttttagaagg
      961 tagctgggat caagaaaaag cggaaaaaat gactagcgaa atcagtaaat tgcatcctga
     1021 aaaccgtgaa ttagatacag gattctttta tattgatcct tctgctatca aataaggagt
     1081 atgatgaaag aaatgacagt aaaaagaaaa ttgaaacgac cagaaatcct agcgccagca
     1141 ggaacactgg aaaagttaaa aaccgcgatc cattatggag cagatgcagt atatattggc
     1201 gggaatgcat atggtttaag aagccgtgca ggtaatttta cttatgaaga aatggctgaa
     1261 ggagttgctt ttgcaaaaga acatgatgcg aaggtatacg ttgctgctaa tatggtcacc
     1321 catgaaggaa atgaagatgg agcaggggaa tttttccgca gtttgcgtga tgtcggtatt
     1381 tctgcagtga ttgtttctga tccagctttg atcgaaattt gtgcgacaga agccccaggt
     1441 ctgccgatcc atttatccac tcaagcttca gcaactaatt ttgagacatt ggaattctgg
     1501 aaagaagaag ggttggaacg tgtcgtttta gctcgtgaag tttcaatgga ggaagtggca
     1561 gagattcgta aaaatacgga taccgaaatc gaagcattta tccatggtgc catgtgtatc
     1621 tcttattctg gtcgatgcac attgtccaac catatgtcga tgcgggatgc aaaccgtggc
     1681 ggatgttctc aatcttgtcg ctggaagtat ggattgttcg atatgccttt tggtcaagaa
     1741 cgaaacagtt taactgatac aggcgaagtg gaagaagaat tctcgatgag tgcagtggat
     1801 atgtcaatga tcgaacatat tccagaattg attgaaaacg gcgtagacag cttcaagatc
     1861 gaaggaagaa tgaaatcgat ccattatgta tctacggtgt caaatgtcta taaagcggct
     1921 gtagacagtt atatggaaga tcctgaaaac tatgtctgca agcaagaatg gatcgacgag
     1981 ttatggaaag tagcacaacg agaattagct acaggttttt actacaatac accaactgaa
     2041 aacgaacaat tattcggtga acgtcgtaaa atcccagtat ataaatttgt cggtgaagta
     2101 ttatcttatg atgaggcaac acagattgct acgatacgtc agagaaactt gttttctgta
     2161 ggagatgaaa tcgaatttta tggtccagga ttcagtcatt tcagtcaaac cgttgaagtt
     2221 atgcataatg aagaaggcga atccattgac cgagcaccaa atccaatgat gattttaact
     2281 atgccagtcg cacagccggt gaaacctgga gatatgatcc gaaaacgtaa ataaggaaaa
     2341 agctgatata tccctgtttt aagcatcttc gcatacaaaa ttcaagaaaa gtgttacact
     2401 tgttttctgt agaaaaagga tcgttaaata aaggttttga ttgatctctt caataaggag
     2461 gaacaaaatg caaatatatg attacatcgt aattggtggc ggctctggcg gtattgcttc
     2521 agcaaacaga gcagggatgc atggcgcaaa agttttgctg attgaagcga atgaattggg
     2581 cggaacatgt gtcaacgtcg gttgtgtacc taaaaaagtt atgtggcaag caagtacgat
     2641 tttggaaaca attgaacgag atgcctcaag ttacggtatc caagcagatt taaaaaatgt
     2701 tgatttcgcc ggtctagtag aaagacgaga aaaatatatt gattttctac acggtgctta
     2761 tcaaagagga ttagatagca atcatgtcga ggctataaaa ggttacgcta cttttatcga
     2821 tagtcaaacg atcgaagtaa acggtgaaac ctatcgtgct cctaaaatct tgatcgctac
     2881 tggaggccgt gcaagtaaaa tgaccattcc tggtggagaa tacgcaattg attcaaatgg
     2941 attttttgaa ttaacagagt tacctaagag agcaatcgtt ttaggtgctg gatacattgc
     3001 ggctgaactt tcaggtgtat ttcgtggttt aggtagtgaa gtcatgtggg catatcgaaa
     3061 agaacgacca cttagaacgt ttgataaaat gctttcggac aatttgatcc aaatgtacca
     3121 agaagctgga ataaaaactt acgcacatca tattgctaaa gaaattacta aaaacaatga
     3181 cgaatatacg gtgctatttg aaaatggtga aaccttaact ggtgattgtg tactttttgc
     3241 cggtggacgt gtaccgaata ccgaaaaatt aggtctggaa aatacaaatg tggaattgga
     3301 caaaaaaggg tttatcaaag tagataaata ccaaaataca acggatgaac atatttttgc
     3361 tgttggtgat gtgatcggta aattagatct gactcctgtt gcaatcgcag ctggtagacg
     3421 tttatcagaa cgtttattta atggaaagaa agattcttat cttgactata aacttgttcc
     3481 gacagtcgtc tttacgcatc caccaattgc aacaattggt ttgacagaag aagaagcatt
     3541 ggaaaaatat ggcgaaaatg aattgaaagt ttatcgttca cgctttaccc caatgtactt
     3601 tgcattaaat gattatcgcc aaaaatgcga aatgaaattg atctgtgtcg gaaaagaaga
     3661 aagaatcgtc ggcttacatg ctattggtat tggtgtcgat gagatgctgc aaggttttgc
     3721 cgtggctatc aagatgggtg caactaaaga agactttgac aatacagtag ccatccatcc
     3781 aacaggagcg gaagaattcg taacgatgcg ataatttaat attaacagtt ccaacagctt
     3841 tttattgaac aaaccatgta aaaccagaaa atcagtatac tgattttctg gttttttgtt
     3901 ttttagttat ttatgtaaga ttgcttcgat aattctcaaa acgaaataag tagggaagtt
     3961 ggagattttg attatcttcg taactgacga agagtaaatg aatccatttg gtatgcactc
     4021 ttaagttcaa aatattggta tcgatgttta aaagaaatag aaatgtatcg aagaacagat
     4081 gtttaaaaaa gtgtgacttt gtataaataa attagaagaa gcaaacgagg atgttaaaag
     4141 aataccatat agttaccaaa ccaagaaagt tctcgtcaag ttcgctggta cgaatcaaat
     4201 caagtgaaaa aagggactgt gaaaaaaatt gtgtcatagt tagtcacttt ttccgaacaa
     4261 atagtgttca atcagctgtc cttcggtatt agctcaaaaa acggcaaaat atgaggaact
     4321 atttttccat ttgtttctct aatactcagg acaaaacact gattttacaa cctctgataa
     4381 acggtgttca aaagccagtt tctcggtatt aagccaaatt tatgcaaaaa tgtggaaagc
     4441 cattttcaca aaatttttct taatactcga aactaagcga ctttttcaca acctcctgat
     4501 aaacggtgtt caaaagcttg catctgcggt aataagccca accaaacgaa aaatataagg
     4561 agctattttt atttggctgt tcttattact tgatgcaggg cagttttttc acaacctctt
     4621 catgctgtta aatggcttgc ataatgattg ttagtaatag ccccaataca taactggcat
     4681 tgaataagac catatttttg atggaatagc tgaaactgat aggctggggt aacgtttgct
     4741 tgaattgatc caaatgcttc ttcacaattg gcaaggtcaa gaaggtgacc aagatcggcc
     4801 attcatagat accgctcaac aaccccatta aaatcacgaa atagcaagca tacatcaata
     4861 gctggaataa gactgttccc gcaggacgtc cgatataaaa aactagtgta tagcgatgat
     4921 tttcaatatc acggtcaaga tcccgcaaat tattagccaa catgatattg gcaatcgtaa
     4981 acaccaaggg taaggaagct aatacgattg ctgctatagc ccaaagattt cctaccaaag
     5041 caaattgtcc agatgaaaag ttgactgtca gatcatagac atctgaaccg aatgtattga
     5101 cataaacgac catggcaaaa attccaaggc ccatagtcac cccactgaaa atttccccta
     5161 aaggcattcg agataaagga atcggtccga atgtatagaa tactccaata aaacaacaga
     5221 taaatcccat caaaagtaaa agccaacccg tattgatcgt tagaataacg ccgataacaa
     5281 aaacaaatgc aatcatagct aaaatcatgt ttcgaatcaa tacaggagat agatttctgc
     5341 ggccaatgac attttcttca tacttatata cttcagaatg agccttttca taatccatgt
     5401 aattattgat agcggtagtc gctaaatcaa aaactagcat tccaataaaa aataaaaaag
     5461 tgtttagcca atgtatctca tgaaaaaaag cgatagagaa taatacccct accgcaaaag
     5521 gaaatacact agccaccttt gttcgtattt caactacttc taaaaagatt tttagtgtca
     5581 tggttcgtac tccttatttt agttcagaac ggtcacccat cgtgtaatgg gagtcctccg
     5641 ataatttgaa ggtgtctttg attggatcgg tcacatatac tttatcgtct tttgtcacga
     5701 agatcgcttc cgtcccgtta ttcaattcgc tttcgacgta ttccagtcct tttttcacac
     5761 ccattgaaaa gactgctgta gacaacccgt caccatcaat tgatttatct gttatgatcg
     5821 tgacacttgc gatatcattg tcgaacggat aacccgtttt tggatcaaac aaatgatggt
     5881 attttttgcc atctacttct aaataacgct cgtaaattcc agaggtaact aatgttttgt
     5941 tccgttcttt aatagaacct aaaacagatc cgcgagccat gttcggatct tggatgccga
     6001 ctgtccagtc ttggttttcg cctcttgggc tgtggcctaa gacatagaca ttaccgccca
     6061 agtcaacgat ggcagtggtc actccttgtt ttttcaagac tttcactact tcgtccgtaa
     6121 tgtaaccttt ggcgattgca cctaaatcga tgatcattcc tttttctttt agataaacag
     6181 ttttttcttt gtcatttagt tcgatcttat gataatccac taatttcaat gcctgatcga
     6241 tttcttcttg ggaaggtttt ctggcgtcat caaaaccgat tcgccataat tgtgtgattg
     6301 caccaatcgc catatcgaat ccgccttgag aatcttggct atattcgtag gcccgcttga
     6361 ctaacgtgta gacgtcatca gaaactttta ctggtttgat cccagcttgc tcattcactt
     6421 catcgatctc agaacctttt tgattgatcg tgattttatc accaagttct tttactcgat
     6481 cgaaggcagg tttcaatgct gattctttgc cttcatcata cacacggata cgaacgtaag
     6541 tccctaacaa gaactgttca tcagaatatg gatctttcaa tatcttggta gctgctgaac
     6601 tttcagtggt tgttttattt gctgagttgc tgcagccagt tagcaccata aaagagatgc
     6661 ccagtgtgat taaggaaaat agtagttttt tccgcatctt tttcctctct tcattttgaa
     6721 aggtgagacg actgcctaat ctggctatcg tcccaccctc caacttttat tagtatgatt
     6781 atgtgattat ttttctgtta cgatattatc tacttcgatt gtttgagtgt cgcctttttc
     6841 agctgcattc actaattgtt gtgcgtacat gatgaatgaa tgtgagctgt gtgtagcacc
     6901 tgtaacaact tctacattag caggtgagcc atcttcttcg cccattgctt tgacgaattc
     6961 gtcgttcaga gtagggatgt aagtttttgg ttctgtaccg ctttttgctt tcatattttc
     7021 attatattct gtatcatctt gttttgattt accgtctttg tcgatataat caaagtttga
     7081 ttcagttact ttgccgccag caactgtcat ttcgaattgc acacggtagc cgttagagta
     7141 gttcttttct tcaagcttgt aagtgccgtc tttcaaatca gcaccattgt cgatttcgat
     7201 tgtatctgtg ttaccagctt gagcagcttg gatcaattgt tgtgcgtagt tttggaagct
     7261 ttcagatgag tgagtagctc cagttacgac ttccacaccg cttgcgcttt gtgcttttaa
     7321 gaattggtca ttcaactcag gaatgtattt ttctgggttt gtgccagatt ttgcttccat
     7381 gtttttgttg tattctgcat cttttgtttt tgattcgccg ttttcattga cgttatcata
     7441 ttttgattca gtgattttgc cgtcttttac aaccatttca aagactgcac ggtaaccatt
     7501 tgaataattt ttttcttcta atttatatgt gccgtcttgc aaatctccgc cagctacaac
     7561 ttttgcagat gattctgttg tgctttttgc agtcgaagat tgtgctgtag atgattcaga
     7621 agaagagttt gagcttccgt tgtcatctgc gccgcaagcg cctaacacta gagctgaaaa
     7681 tgctaatacg gcaaatccag ttacaaaacg ttttttcttc attttaatac cccaccattc
     7741 catttctagt ccacaatttt tctttgtgat tcattataca ttctaatctt tttagcctat
     7801 aagtcaacta tttttgataa ataacccatg taatatttca aacgttttct ttcagcaaat
     7861 ttcgtgaaaa aacgctttat tttaaaattt gaaaaaaatc caaaaggagt tagacaaaag
     7921 aacagaacat ctatataata tatggagatt gtgtaataag tatcaaacta aaagaattat
     7981 catatattca ttatatagat agtctataag gagcggatga tcaatggcaa agcaaaatat
     8041 tgtcgttgta ggagcaggat atgccggtgt ttcagctaca aaatacttgg caaagaaact
     8101 gaaaaaagaa gatgtagcaa ttactctgat cgatcgtcat tcgtaccata ctatgatgac
     8161 cgagttgcat gaagtagctg gcggacgtgt agaaccagaa gcaatccaat acgatctgca
     8221 gcatttgttt gctcgtcaaa agaatgtaca acttgtcaca gacactgtaa tcggtatcga
     8281 taaagaacga aaaatcgtca agaccaaatt aggttcctat ccttttgatc agctgatcct
     8341 tggaatgggc ggagagccga atgactttgg tacacctggt gtcaaagcac acggattcac
     8401 cctttggtca tttgaagatt ctgtaaggat tcgggaacat attgagaaga cagtagcaaa
     8461 agctgcattg gaaccagatg atgaactgcg taaagccatg ctgactttcg ttgtctgtgg
     8521 ttctggattt accggtattg aaatgattgg ggaactgatc gactggaaaa atcgcctagc
     8581 caaagattat aaattagatc caaacgaaat cacactgatg gtcgtagagg caatgccgac
     8641 gatcttgaat atgttggatc gaaatgatgc tgcaaaagct gaacgctatt tgcgtaagca
     8701 caacgtggaa ttattattga atgcaccaat cgtagaagtg gctgctgatc atatcaaatt
     8761 gaaagacgga cgcaacattc cgactcacac actgatttgg acagctggtg tcaaaggaac
     8821 ttctgacgct gctgactttg gattagaatc agcacgcgga cagcggttga tcgccaatga
     8881 atatatgcag gcaaaaggct acgaagataa aaacatctat atcattggtg atttagtctt
     8941 ttatgaagaa ttcccaggaa caccgacacc gcagatcgtt caagctgctg aacaaacagg
     9001 acacacagcc gcagcgaata tcgtggcttc gatcaaaggc ggagaaaagc ataaatttaa
     9061 aggaaactat caaggattta tggtttctat tggtgccaaa tggggggtag ctaacctatt
     9121 tgataagatc catttaagcg gatttttagc tatcgtaatg aaacatatcg tgaatttgaa
     9181 atacttcttt gatattcgtt ctggttatta tatgttccaa tatttaatgc atgagttttt
     9241 ccatatcaaa gatgatcgca acgtgactcg aggacattct tctcgttacg gcaatgtctt
     9301 atggagtgtg ccacttcgta ttttctatgg tttagtttgg ttcgttgaag cttccaaaaa
     9361 aatcattggg aatggtgaag tgttcaaacc aagtacttgg tttggtgaag gatcatggtt
     9421 cactggaaac agtgtgttcc cttggacatg ggaatacaat gtaacagatg ttgccagtgg
     9481 tgcttctgct gctggtgatg caaccacagc tgccagcgga gcaggggcaa gtgccggaga
     9541 agcagcgact caagctgcac attttggttt gagttatgca tacggtgaag aaccaatggc
     9601 tgtgattgag aagtcaccag actggttcac aaagatcatg caagtgatca tgccaaacaa
     9661 tgatgtagca atgttcttcc aaaaattcat ggtattcttt gaaattgcct tggcattagc
     9721 attgattgct ggattattca catggctatg cagtgcgaca acgattgtat tggtgatcac
     9781 gttctgctta tcaggcatgt tttactgggt aaacatttgg ttcatttttg ttgctttcgc
     9841 attgatgaac ggttccggac gtgcgattgg gttggatcgc tgggtaattc catggctaca
     9901 acgtacacta ggtaattggt ggtacggtag accaagttcc cagtacggca agaaataaat
     9961 agataaagaa cgttatttga atgcaagatt tcaatagaaa ggggctgaga caaaccgtca
    10021 cagcctcctt tttatgtaaa attaaaggaa taagatgccc gagataagcc tccatgagca
    10081 agacacaggt gtcacaacct ctgttacaac ctcttgttat aaacggtgtt ccagaagtag
    10141 cttcttcgaa aaataagccg aaatttcaca aaaacttgaa aagtaatttt cgtaaattcc
    10201 ttcttatttc ttgaagttgc ccacttctgt cacaacctct tgttataaac ggtgttccac
    10261 ctgctgcttc tcggtaataa gcccaaagaa ttagaaaaca tggaaagctg ttttctattc
    10321 ttcgttctta ttactcaaag caagacacag gtgtcacaac ctcttgtttt tatgctaaaa
    10381 ttgggtgagt taatctggaa aaaggagaac aaaaatgagc cttaaagaat ttgctgaaaa
    10441 aagtctgctc aaaccttggg atgtttttat tattttgctt ttgactgttc tttctttttt
    10501 accagtcatg atttttggtc ttcagcaaaa caccgcaaca gcagaaaaag aagcggtact
    10561 gcgtgtggat ggcacagaaa tcaaaacatt ccctttgatg gctggagaag agagcaacac
    10621 atatacttac aaagatgaac acggagatta taacttgatc gagatagatg gcgatcgagt
    10681 acggataaaa gaagcagatt gcgatgacca gatctgcgta cgtcgaggct gggcaacgaa
    10741 aaatggcgaa accatcgtct gtttgccaca taaattagtg atcgaagtcc gttcaacaga
    10801 tggaggtgac gaagacagtc tgatttatta gaccttcaca caccatgagt aaattacaaa
    10861 aaatgattta tatttcgcta cttgttgcac aaggagtgat cattggttta ttggaaaata
    10921 tgatccctta tcctttttct tttgcgcctg gtgcaaaact aggcttagcc aatctaatca
    10981 cgatcgttgc catttttacc atgaaaaaac gagacagctt cttactactt tggctacgac
    11041 tattcctgac tactttgcta ggcggaacga tttccacatt cctttatagt atgagtggtg
    11101 ctttgcttag ttattttgga atgttattgg tcaagcaatt aggaccaaaa cgtgtcagta
    11161 tcatcggaat tagtgcggcc ggcggtttca tgcataatgt cggacagctt ttgatggctt
    11221 ctttcattgc acaatcatgg acagtcatgt tgtacttgcc agtcctttca gttttaggga
    11281 ttttatcagg gattgcaatc gggatcgcag cgaattatct tttacgacat gtgcagacac
    11341 ttcaacgatt ccaaaaagat tatgagcagg gaaccaaaca aaaatggagc ccaatgttta
    11401 ccaaaaaata atcgtcacac aaggagtttt tttatgaagc tacatccaat gtgggaaaac
    11461 tatcctgctt taagtaaaga attgacagaa acactaaaac ggatggaaga aactgtccgc
    11521 ctgaaaaaca aacctgtcga acaagcaatc aaagaaacga tccatgccgg cggaaagctg
    11581 ttgcgtcctg cctatcagct attgttctca caatttggac cagagcaaga tcgagacaaa
    11641 gccatcgctt tggcagcttc tatcgaaatg ctccatacgg ccacactgat ccatgatgat
    11701 atcgttgatg aagcggatct gcgacgtggt cagcctaata tccgtagtca gtttggtaat
    11761 gtcgtttctg tctatgcagg agactattta tttgtctgct gttttaaact attgtctgat
    11821 tactccactt ccttgaaaag tctccaattg aattctcgca gtatggaaaa agtattgaat
    11881 ggcgaattag ggcagatgga tgatcgctat aattatgaac tttctgtaaa agaatatttg
    11941 gaaaatattt caggtaagac agctgaactg ttccaattaa gttgttctgt tggcgctttt
    12001 gaaagtggaa caagcgagcg cttcgcaaaa aaagcaggag atatcggact ttctatcggg
    12061 atggcctttc agattatcga tgatatttta gattatacaa aagagagcca ggaaatcggc
    12121 aaacctgtac tagaagacat gcgtcaaggg gtctattctt taccgttgat ctactctttg
    12181 caaaaaaaca aaccaaagct actaccttat ttagagaaga aagcggcaat gacagaagaa
    12241 gatgtcgacg ccgtacgcaa aatcgttgaa catacaggag gcgtggaaga agctaggaag
    12301 ttagcggcct cttacacaga aaaggctttg aaggaaataa agaaattacc tgccacctct
    12361 cttcgaacaa aagagaatct cttttccctt acccaactga ttttggatcg caaagactga
    12421 atagggaaag gttcgatacg taaataaatt tctcagaagg aaatctacgt gttttttgac
    12481 aaattgaatt gtagttcttg tatttatgat aaaaagcaaa aataaagagc aacgtccgtt
    12541 agcgacgttg ctctttttta tcttgaatat ttttttacct tttcgatttc ttataactca
    12601 taaaagtgat tcttaaaccg attacaagta atataacgcc tgcgagtgtt atccaattat
    12661 tcgattgact tccagtttta ggaagtgctc tttttgattt gtttccattg tcattgcttg
    12721 ttttattagt taagttaaag ttcgtcctgt tatccgaact tgtttttgag aaggagacac
    12781 ttgcatgacg tgtatcgctt gtggtagacg acgtgtctgt actatcgctt gaagttgatg
    12841 aatctgagct atcacttgtg gtagaggatg tatctgtact gtcacttgaa gctgacgtgt
    12901 ccgtgctatc acttgtagtg gtagatgaat ctgagctatc acttgtggta gaggtagtat
    12961 ctgtactgtc acttgaagct gacgtgtccg tgctatcact tgttgtggta gatgtatccg
    13021 tactgtcgct tgaagctgac gtgtccgtgc t
//
LOCUS       DAZBSA010000081        13027 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000081 DAZBSA010000000
VERSION     DAZBSA010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.081"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            410..979
                     /locus_tag="ACHGMT_002110"
     CDS             410..979
                     /locus_tag="ACHGMT_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFN6835840.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            966..1826
                     /locus_tag="ACHGMT_002111"
     CDS             966..1826
                     /locus_tag="ACHGMT_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFN6835841.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2016..2720
                     /gene="yycF"
                     /locus_tag="ACHGMT_002112"
     CDS             2016..2720
                     /gene="yycF"
                     /locus_tag="ACHGMT_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFN6835842.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2725..4560
                     /gene="walK"
                     /locus_tag="ACHGMT_002113"
     CDS             2725..4560
                     /gene="walK"
                     /locus_tag="ACHGMT_002113"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFN6835843.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4557..5873
                     /gene="yycH"
                     /locus_tag="ACHGMT_002114"
     CDS             4557..5873
                     /gene="yycH"
                     /locus_tag="ACHGMT_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFN6835844.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5874..6743
                     /locus_tag="ACHGMT_002115"
     CDS             5874..6743
                     /locus_tag="ACHGMT_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFN6835845.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6798..7607
                     /locus_tag="ACHGMT_002116"
     CDS             6798..7607
                     /locus_tag="ACHGMT_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFN6835846.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7710..8339)
                     /locus_tag="ACHGMT_002117"
     CDS             complement(7710..8339)
                     /locus_tag="ACHGMT_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFN6835847.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8509..9801
                     /locus_tag="ACHGMT_002118"
     CDS             8509..9801
                     /locus_tag="ACHGMT_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN6835848.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9877..10539)
                     /locus_tag="ACHGMT_002119"
     CDS             complement(9877..10539)
                     /locus_tag="ACHGMT_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFN6835849.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10764..11048
                     /gene="groES"
                     /locus_tag="ACHGMT_002120"
     CDS             10764..11048
                     /gene="groES"
                     /locus_tag="ACHGMT_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFN6835850.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11099..12724
                     /gene="groL"
                     /locus_tag="ACHGMT_002121"
     CDS             11099..12724
                     /gene="groL"
                     /locus_tag="ACHGMT_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFN6835851.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4338 a         2235 c         2803 g         3651 t
ORIGIN      
        1 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
       61 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      121 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      181 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      241 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      301 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      361 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      421 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      481 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      541 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      601 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      661 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      721 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      781 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      841 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
      901 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
      961 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1021 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1081 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1141 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1201 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1261 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1321 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1381 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1441 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1501 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1561 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1621 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1681 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1741 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1801 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1861 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     1921 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     1981 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2041 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2101 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2161 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2221 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2281 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2341 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2401 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2461 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2521 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2581 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2641 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2701 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2761 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2821 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     2881 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     2941 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3001 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3061 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3121 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3181 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3241 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3301 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3361 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3421 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3481 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3541 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3601 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3661 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3721 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3781 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3841 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     3901 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     3961 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4021 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4081 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4141 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4201 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4261 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4321 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4381 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4441 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4501 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4561 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4621 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4681 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4741 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4801 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4861 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     4921 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     4981 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5041 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5101 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5161 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5221 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5281 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5341 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5401 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5461 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5521 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5581 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5641 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5701 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5761 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5821 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     5881 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     5941 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6001 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6061 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6121 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6181 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6241 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6301 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6361 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6421 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6481 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6541 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6601 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6661 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6721 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6781 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6841 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     6901 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     6961 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7021 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7081 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7141 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7201 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7261 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7321 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7381 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7441 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7501 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7561 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7621 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7681 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7741 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7801 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7861 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     7921 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     7981 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8041 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8101 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8161 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8221 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8281 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8341 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8401 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8461 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8521 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8581 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8641 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8701 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8761 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8821 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     8881 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     8941 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9001 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9061 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9121 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9181 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9241 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9301 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9361 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9421 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9481 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9541 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9601 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9661 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9721 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9781 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9841 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
     9901 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
     9961 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10021 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10081 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10141 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10201 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10261 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10321 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10381 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10441 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10501 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10561 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10621 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10681 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10741 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10801 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10861 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    10921 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    10981 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11041 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11101 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11161 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11221 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11281 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11341 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11401 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11461 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11521 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11581 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11641 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11701 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11761 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11821 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    11881 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    11941 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12001 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12061 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12121 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12181 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12241 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12301 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12361 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12421 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12481 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12541 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12601 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12661 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12721 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12781 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca
    12841 aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc tgtttatacg
    12901 tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt tttgtgtgta
    12961 attataatga aaatagttat ttttaatagt tataatattt tatctattaa aagtaaatat
    13021 ccattca
//
LOCUS       DAZBSA010000082        12893 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000082 DAZBSA010000000
VERSION     DAZBSA010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.082"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACHGMT_002122"
     CDS             476..1339
                     /locus_tag="ACHGMT_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFN6835852.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACHGMT_002123"
     CDS             complement(1468..3090)
                     /locus_tag="ACHGMT_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFN6835853.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACHGMT_002124"
     CDS             3339..3860
                     /locus_tag="ACHGMT_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFN6835854.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACHGMT_002125"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACHGMT_002125"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFN6835855.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACHGMT_002126"
     CDS             5941..6759
                     /locus_tag="ACHGMT_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFN6835856.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACHGMT_002127"
     CDS             complement(6862..7059)
                     /locus_tag="ACHGMT_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835857.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACHGMT_002128"
     CDS             7228..7806
                     /locus_tag="ACHGMT_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFN6835858.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACHGMT_002129"
     CDS             7901..8038
                     /locus_tag="ACHGMT_002129"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835859.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8851..9354)
                     /locus_tag="ACHGMT_002130"
     CDS             complement(8851..9354)
                     /locus_tag="ACHGMT_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFN6835860.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9713..10738
                     /locus_tag="ACHGMT_002131"
     CDS             9713..10738
                     /locus_tag="ACHGMT_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFN6835861.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            11061..11939
                     /locus_tag="ACHGMT_002132"
                     /pseudo
     CDS             11061..11939
                     /locus_tag="ACHGMT_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
     gene            12415..12531
                     /locus_tag="ACHGMT_002133"
     CDS             12415..12531
                     /locus_tag="ACHGMT_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFN6835862.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
BASE COUNT         4022 a         2218 c         2472 g         4181 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaata acatatctca
     8341 ttttacactt ttgttatttt atacttaaaa aataacgaca cagaaaatac cgtgtctcag
     8401 atttctctac tacttctaaa aactactgtt tttttccttt gaaatgcctt caaaaagtag
     8461 gatcaggcag aatatactgg cagaatcaag gagaagggag cctaaataag gagaaaaacc
     8521 atagaaaata taaagtaatt acttataaaa caagcttttt atagaaaaat aatatacctg
     8581 agtagggtat aaattaagac ggaattcctt tctagattga gatattgact aggaagaatg
     8641 ttaagatttt aagcctttaa gctaaaaaaa aacccttttt gttaaataag gggaatatag
     8701 cctgtcagtt tcaaactgca agtcgtcctg ctgcaataat atagtagatt agagatgaaa
     8761 atagattttt ttaaaccaat gactgaataa aaagaccgtc atagatacac attggaatgt
     8821 gtctattacg gtcttttttt acgaacgatt ttattgaaga cggacagttc gggtcaaaaa
     8881 taaaaaagct acgatcagaa ataagaataa aatgtatccc aaaataatgg ctagacggtc
     8941 taacttattt gtcgatagat gtttgtttag aagttgatag gcccataaac ctatcagtcc
     9001 tcctgttgta ttagttatca aatcagtgat gtccgctacg ccaataccga ataggaattg
     9061 aatcaattct gccaagaagc ttacaaggaa aataatccct aattttttag aaaaagaaag
     9121 tctcttgaaa ttgacattca aaagcacccc aaaaggaaaa aagacaatca agttataagt
     9181 ggtctcacgt aataccgtcg tattttcaaa agaaaaagta gaaaaaggga cgagattgac
     9241 attgctgtaa gaataagtga gaaattgtgg aagattaaat gatagcttga ataaaatcaa
     9301 ccataataga aagaataaat aaatccctaa taaaaatttt gatacatacc gcatagacat
     9361 ctcctccatt taaaaagtat accatcgaaa accgctgaaa aatcataaaa aagtatgaag
     9421 tttaaggacc aacgtagtta tcgtacgaaa attagaagct tttttttgtt gggaatccgg
     9481 tttctttaac aaagctaaga tagcgagttg cttggaaaag ttgctatctt ttttctgtca
     9541 aaacgactcg gaatgttcgt tttttgttct aatttctcca agttgtttac tttttgatac
     9601 taagtttatg aaaaatcaag aaatagttgt tatactaggt aatgctgaca aacgggattg
     9661 gcatttcatc ttggttcagc tttttttcca attactagga aggagggata gattgttcaa
     9721 gacttatttt cgtttcaata aaatacaaga taatcctttt cgtatcatga atctgatgct
     9781 acttatcttt attgtattat ggttaggtta cataaatcat gattttttga tttcaagttt
     9841 ttcatcatta gggatcttta cttttttcta ttatcaaaac ataccgatga aatatttgct
     9901 gaaacgtatg tttctgatcg gttgcggact gttggttgct ttcgttttgg ggatcttatc
     9961 gacttatgtt ttatggcttg aaccatttgc cgtagctttg gttgcttttt ccagcagatt
    10021 cgtgttgcga ttattccata tttctaaacc aggtggcttg ttttttgcga tgctttctgc
    10081 catgggaacg agcatgcagc tgccaatcgc tcaattaccg atcgtctcac tttatttttt
    10141 tatgggtgtc gtttttgccc tgattgcggc agtgattaca aaattactgg attcacggcc
    10201 tgagcagaca attgaaaaag caactttaaa agaaagattt cacgaagagc ctttagtgat
    10261 cattgattct gttttctaca gcgctgcctt atttctttcc gtttatgtca gtcacggctt
    10321 gaatctgcat aatccttatt ggttgacctt gtcatgtgca tcgattttgc tagcagaaaa
    10381 cttggatgcg atgaagcatc gacaagtcca atatctgatt ggatcgatgg gtgggttatg
    10441 tgtgtcggca ttcttgtcat ttgtgccgtt tacgcagttg cagaccattt ttctgatcac
    10501 ttttctatat ggaatcgcac aatttctagt tgctagaaat tatgcagttg ctaatatttt
    10561 tcttaatccg atggcgttga tgctatctac acttattcgt ggagcatact tgatttcttt
    10621 gatcgaatat cgttttttag ggatcgtcat aggaagtttc atcggtttag gtgttgcgtg
    10681 ggtaatgacg gttggtttgc aacattattt aacggttgtt cgtcaaaaat tagaatagaa
    10741 ggacaaatat atgtgccaaa agaggttatg acaagatttt tgtcacaacc tctaattcaa
    10801 ggtgttcaaa agccagattc tcagcattaa gtcaaattca cacaaaaatg tggaaaggaa
    10861 ctttcaaaaa aattttatat ttattctatc aaaaaatttt gtttatgatg gtaactgaat
    10921 aaactttcct gagatggtca atgctttcct tgaaagtctt acagatattc tttcttcata
    10981 gaaagtttaa gttttatagt tagtgtacaa taaaagtatg tttataccat atggtgtccg
    11041 gataatcagt catttaaaca atgaggttca aagatatgaa aaggggaaat ttgatgaaga
    11101 aaataagcaa tgaaatccaa attctggcag aaactttaac tcttcccaaa gtcgttatag
    11161 agcaaatgat tgtacaagca aaaaacatcc ttgatgaggt gtttgagcaa tatagtcatg
    11221 aactaatgga tcctaataaa gcaaaggcga ctgcaaagaa aatagacaaa gtctatgaag
    11281 aggaaccttt tgcagcatta gctatttatc tatatgcagc ttctcaaagt tggaaaaaaa
    11341 tttatcaacc attgaagatt ccaagaacta tctatttggc aacaatgaat gcttttacta
    11401 gatttattca tgaacatttc caagtaacag gaagctatcg ctttgaccgt ggtttttgga
    11461 tttggcatta cactagtggc ttgatctttc ggattggtga attggagtac gaaatgacat
    11521 attttccgaa agggtacacg gatctaagat tagaaggaaa gcagtgcctt tctcttcata
    11581 ttccttccga tgcagattta tctcagaata agataagaga tagttacgaa agtgccattc
    11641 gtttttttga actttatttt cctgattacc attaccaagt gatgtacaca gatacttggt
    11701 tactatcacc caatcttact aaatagttga aaaaagagtc aaaaatctgt ctttttgcag
    11761 ccgattatcg attattatca gttgatgaac aggacgatag tggagtccct tggatctttg
    11821 gacgagtaga tgcccaaata catgattatc cagaaagtac gagtttacag cgacaagcaa
    11881 aagagcaatt gcttgcaggc gagcacattg gctctggctt aggtattctt acttcataag
    11941 tttagtactt cgaaatagtt taaaagagcg tgagacaaaa gtaaagaaca cttttgtctc
    12001 acgctctaaa cacgtataaa cggcggaagc agaagcaact ccttcggaaa taagctgaaa
    12061 ttcacaaaaa tttgaaaagc aattttcgtg aatttcttct tatttcttgg agttaaacgc
    12121 ttctgtccca gcctcttttt tagtgttcta tcgaacacac agtacgttca ataggtaaac
    12181 tagctataac aatagatatt aaagttaacg gacaagaggg ccttttttag gtagctaaaa
    12241 cgtaataatg aaaatttatt tccagagata ggagtcaatc agctagtctg atcgactgtt
    12301 ttaacaagtt gactttcatt ttaacttttg ataccattta aaacacaaat cgtaattgtt
    12361 tcgatttgtg ttttttagca tgactacaat caaagaagaa agtgggtcgt ccatatggaa
    12421 aaaacagata tctcaagtgc ataccgccgc ttgaaaagcc caaatatcaa aacacgaaaa
    12481 cgtgcgctga agatcatcaa agatgtgaaa cggaatagtg ggaaaaggta agaagaagga
    12541 aacttaatta aaaaaatagc cgaaaaacgt ttttcggcta ttttcaataa tcggtttttg
    12601 tgtaaggttt ccgaaagaac tgctgttgat tttccatttt taacatatga taaattggta
    12661 gaatctttct tttcaaattg ataccattcc ctaattccag ttatatagtt aactacagaa
    12721 ccacaaagca cgtacgttta taaaagtatt atctgtagtt atttttcaat tccctttcaa
    12781 cctcttattt tgaccagtag tattgatcac tttacctttt aacacctttg ttcgtttttg
    12841 aaacattttt agtcaagaat ggaaacgtaa ctacataatc aaaggttttg cga
//
LOCUS       DAZBSA010000083        12793 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000083 DAZBSA010000000
VERSION     DAZBSA010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.083"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..3654
                     /locus_tag="ACHGMT_002134"
     CDS             127..3654
                     /locus_tag="ACHGMT_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFN6835863.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="ACHGMT_002135"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="ACHGMT_002135"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFN6835864.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="ACHGMT_002136"
     CDS             7442..7783
                     /locus_tag="ACHGMT_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFN6835865.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="ACHGMT_002137"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="ACHGMT_002137"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFN6835866.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /gene="pheT"
                     /locus_tag="ACHGMT_002138"
     CDS             9140..11560
                     /gene="pheT"
                     /locus_tag="ACHGMT_002138"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFN6835867.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="ACHGMT_002139"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="ACHGMT_002139"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFN6835868.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         4328 a         2232 c         2717 g         3516 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg ttt
//
LOCUS       DAZBSA010000084        12738 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000084 DAZBSA010000000
VERSION     DAZBSA010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.084"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            97..540
                     /locus_tag="ACHGMT_002140"
     CDS             97..540
                     /locus_tag="ACHGMT_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFN6835869.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(546..1730)
                     /locus_tag="ACHGMT_002141"
     CDS             complement(546..1730)
                     /locus_tag="ACHGMT_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFN6835870.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            1998..2696
                     /locus_tag="ACHGMT_002142"
     CDS             1998..2696
                     /locus_tag="ACHGMT_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFN6835871.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2703..3380
                     /gene="nth"
                     /locus_tag="ACHGMT_002143"
                     /pseudo
     CDS             2703..3380
                     /gene="nth"
                     /locus_tag="ACHGMT_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
     gene            complement(3506..4039)
                     /locus_tag="ACHGMT_002144"
     CDS             complement(3506..4039)
                     /locus_tag="ACHGMT_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835872.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4336..6717)
                     /locus_tag="ACHGMT_002145"
     CDS             complement(4336..6717)
                     /locus_tag="ACHGMT_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFN6835873.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6707..7354)
                     /gene="recU"
                     /locus_tag="ACHGMT_002146"
     CDS             complement(6707..7354)
                     /gene="recU"
                     /locus_tag="ACHGMT_002146"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFN6835874.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7418..7957
                     /locus_tag="ACHGMT_002147"
     CDS             7418..7957
                     /locus_tag="ACHGMT_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFN6835875.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8047..8466
                     /gene="gpsB"
                     /locus_tag="ACHGMT_002148"
     CDS             8047..8466
                     /gene="gpsB"
                     /locus_tag="ACHGMT_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFN6835876.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8488..8855
                     /gene="rnpB"
                     /locus_tag="ACHGMT_002149"
     ncRNA           8488..8855
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHGMT_002149"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9064..10230
                     /locus_tag="ACHGMT_002150"
     CDS             9064..10230
                     /locus_tag="ACHGMT_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFN6835877.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            10276..11772
                     /locus_tag="ACHGMT_002151"
     CDS             10276..11772
                     /locus_tag="ACHGMT_002151"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFN6835878.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            11853..12536
                     /locus_tag="ACHGMT_002152"
     CDS             11853..12536
                     /locus_tag="ACHGMT_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFN6835879.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            12545..>12738
                     /locus_tag="ACHGMT_002153"
     CDS             12545..>12738
                     /locus_tag="ACHGMT_002153"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835880.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         4272 a         2230 c         2630 g         3606 t
ORIGIN      
        1 tcttggtttc agattgggtt ttcctacaaa atatttttat ctttgttgaa taggaaacaa
       61 aaaaacaata taatgaaaga aaaagaaggt gtagaaatgg ataaaagaca actgctaata
      121 gaagaatatc tggatttagc taatgagatt attcatcgta ataaaccaga aatggaaaaa
      181 gaatttgcag gatttactct aaatgaacta gaagtgattg aacatatcgg gaaaatccct
      241 gatcctaacg tgacaaaatt agcgaatgct agttacatga ctagaggagc catcagtaag
      301 ctaacaaaaa aactgatagc tagagggatc ataaacactt atcaaagcga agagaataaa
      361 aaagaaatct attttcaatt gacgccaatc ggacaaaaaa taaggaatag acacgagcaa
      421 ctccatcaaa aatgggtaca gcgagatgct actgtatttg aaaatatgtc aaaagaagaa
      481 tttgacacag tatttcgttt tatcggtcga tacagagaat ttatacgaag tagcaaatga
      541 agctactatt ttttattgta gtgataatag gcaaaaatca taagcaaaaa tccgaatacc
      601 gtcggaagaa atggtgctgc agcataaagc tgtcctgcga ttagcgggcc gatgacgcgc
      661 gatagagatt gtatactttg ggtaccgccc attacaagac cttgctggtt atctgagaca
      721 gaattggata gttggccatt gaatacagga gtaaaaatag aatcaccgaa accataacag
      781 ataatcccaa ataaaaacaa aatcggcagt agaagaattc cagataatgt gatgaataga
      841 taggcgagga tttcactgaa catccctatt cttgtgatct gctgttctga aaattttttg
      901 agcaatcgag gcataatgaa caattgcgag acagtatcca agattccaat caaagagaag
      961 cttaaaccaa ccaacccagc cttccactga aaggcgtcaa tcgaaaattg agaaaagata
     1021 gattgaagag agccagctgc caaccaaaca gcaaaacctg ctgtcaataa aggaataacg
     1081 gatgaaatct tgaataattg ttgtaattgg tgaaaaggtc ttacatggga gaaacttaaa
     1141 tctactgaac gtttttttat tggaaggctt tctagcataa atgtgtagcc ataaacggca
     1201 tttaagaaag taaagagtgc tccaataaat ataggaacag aattccccaa ctcagctaat
     1261 agacccccta taataggacc cagcgttgtt cctataccga ctaaagcacc catccatcca
     1321 aaaactttgg ttctttcatt aggttccgtg atgtcggcaa agtaagcata taaggtggag
     1381 atttctcctg ccgtcaatcc ttcaatgata cgaccaagga acaacatcca tatcgaattt
     1441 cctaaaccaa aaagcaaata tccgatactt gatcctaata aactgataat aaggatcgga
     1501 tgacgcccaa aatgatcact taagctaccg agaatggggg cagataagaa ggcagctaga
     1561 gcataaacag acatgagcaa agtgatatag aaagcttgct gatgtatgga gtgagaataa
     1621 ggcagcgtta aaaacggaag tacaggactg acgatagtaa atcctaatcc agtgaataag
     1681 acagagacgt atccaaaaac tttagaatgt ttatttgtga aactgtacat gtaaaaactc
     1741 ctttatgcaa taatgttaac ttggaaacaa aaatagtgta tagtaatttt gtttccttgt
     1801 caacattcta aggaacagcg aaatctactt aatttattta ttccgaaaag tgaagatacg
     1861 catcaaaaac atgaacaaac aaaaatcaag tagacgacat tgtaaatctg accgatcagt
     1921 gaagtaaata gaaaagaaac ctgttcttgc ttcccatgtt ttttttacgt atactgtgat
     1981 tgagcggagg agaaatcatg ttgaatttag aaaattactt aagcgctgga caaacagtca
     2041 tatctaatct tttacttaaa aattatcata ggatcggtct acaatctgaa gaatttttat
     2101 ttgttctaca gttgcatatg gcacagcttg aaggagatac tttccctgat ttgcagatga
     2161 ttgctatgga tatgggaatc aaacaagatc agatttttca aatactggat cgtctcgtaa
     2221 caaatggatt tatcaagata gaaacgacga ttgtcaacgg taaaaaagca gatcgatata
     2281 atctttaccc catctatgat ctattaggtg agtatttaaa aacacaagaa aaaaaatacg
     2341 aagaaaaaac acaagaaaaa gaaatccagt cagtgtatca attatttgaa caagaatttg
     2401 gacgcccgct ttcttcgatc gagttccagc ggatcggaca atggctagaa gaagaccatt
     2461 atcagccgga aattttgaag ctagcactgc gggaagccgt attaaatcag gcttatagtt
     2521 ttaattacgt tgaccgcatt ttgctatcgt gggaaagaaa aaatctacgt acaaaacaac
     2581 aagtggaaga agaccaaaaa cgaagaaaac agcagcttct tcaaagagaa gtagaacagt
     2641 cagctcaaca agaagaattg ccgaaagtct cactgaaaaa ttggttggag gagtagcgga
     2701 atatgttaag caaacaaaaa acaatggaag ctctagaaac gatgtatgga atgtttcccg
     2761 aagcacatgg agaactcaag cacaataatc catttgagtt attgattgca gtgatcttaa
     2821 gtgcccaggc gacagatgta tcagtgaata aagcaacgcc agatttgttt gcctcttttc
     2881 cgacacccga tgcgttagca gaagcttcaa tcgatgaaat cattcttaaa ataaaaacga
     2941 ttggtttgta tcgaaataaa gcaaaaaaca tcaaagcgtg tgcacagcag ctgattgaac
     3001 gtttcgatgg gcaagtgcca acgtcaagag aagaattgat gtctcttcct ggtgtaggca
     3061 gaaagacagc aaatgtcgta ttgggggatg cattcgggat accagcaatc gcagtagata
     3121 cccacgtgga acgtgtctca aaacgcctga ggatctgcaa actagatgct acggtcatgg
     3181 aagtagaaga aacactgatg cggaaagtac cacaagaatt atgggtaaag acacatcata
     3241 cactgatttt ttttggttga tatcattgca cggcaagaaa tcccaagtgc gaggtatgtc
     3301 ctttgctatc aatatgtcaa gatgggaaaa atcggatgcg tctaaaagaa aaggcgctaa
     3361 aaaagaaacc acgcctgtag acgtggtttc tttaattgca aataaatgta tgaattatct
     3421 tgttattatg gagaaaagct gtttcgttta tcttttttgc atgaaaaagt aaatgtacat
     3481 actcagtagc taggatggta acttattaca atttattgcg ataaaaataa aagttgttat
     3541 ttttattggg aaaatacccg atgtagaaaa taatgccttc caatatacct ataaaataag
     3601 tccagtattc caaaggatta tataaataag tgacgtatct atctaaaggt gtaataaaaa
     3661 tgataagtga taataaaaaa ataccattta taactaaaca taaactaatg gatttgggcg
     3721 gtttcaggct catcagaaaa atatagattc tggtgaacaa caaccataaa aaagtgagaa
     3781 tcattgcatg agagcttctt tccataaatt ttcctttaag agtagtgctt aaaagtacag
     3841 aaaaaataat aaatgcaagg ataggagcaa ttaaagtaaa tttatttaat aataaacaaa
     3901 atttttcagc ttttttattc ttggaataac gaggtattgt tttcgataaa aaaagatggt
     3961 gcagacaaat tatgataatt aagcatacta tcagataact caaaatgaag ccttctttca
     4021 aaaaattatt caaaaccata tattgtattt tttatttgat aataacaatg ggattattaa
     4081 gaagtggcac taaataaaag actagtgtgt atttagccga tttaattata aacaagtaaa
     4141 tggataaagt atatgtaatg ataaaagaaa aatggatttt ctaattttta agctaaatgt
     4201 tagatcacac atgtatatgc tggatagtct tataaaaaat catgtaatac agcttggtac
     4261 atgattttta tttgtagctg ttttttacat agacacaaaa aagaaaccac gcttgtagac
     4321 gtggtttctt ttttactatc tgtttccaga agctgaggtg ctgctggatg gtgtatggtc
     4381 atttgcagca tccgctccgc tagttgctgg ctcactggaa gaagcaggag gagcagatga
     4441 agacggtgtc gagctttctt cggatgaaga agaaggaacc gttgttgaag acgctggctc
     4501 gcttgtttct gctgtcgtgc tttcttttga ggactcagca gttgattcgc tttgactagt
     4561 agagctagag ctttgagtcg ttgtctctgt tgttgagctt ccaggagtct ggacatagct
     4621 ggaagatgga atagttgttg atggtgtaat tgcattcgat ctagctgtat attgatcttt
     4681 gtagtacagt tcaccgccca cacgaattaa tccgcttggc atctcccagt ctgtatttgt
     4741 cacgttagct gaaacatact gcataagctc gcgataaaca tcagaggcta cgtgtgacga
     4801 ttctgaagtg acaggcgtca tttttttatt gtaacctgtc cacacggaaa tcgaataatt
     4861 tggcgtatag cccgcaaata ggatatctgg atacacccca gaagaaatac caagttttgc
     4921 atactcgtca tctgtgtaat tggatgttcc ggtcttaccg gcttgatata acccagcgat
     4981 ttgcgcattc gtaccagttc cttcagtgat cgtatctttt aaaatgtctg tgatcatata
     5041 agctgtttct ggagacatgg ctgtttttcc atctggctca taagtttctt ctgttccgtc
     5101 ttggaagacg attttattaa catattgagg tttgtaataa gtccctccat tggcgaaagc
     5161 cgcataggct gctgccattt ttaaggaaga agctccgtat tttgtcccat cttgttcttc
     5221 cgtattacta gagatcgcat tcgattgatg gatcgtactg tattcgatcc caagattttt
     5281 taagaaagaa gccactttat ctgaaccgac ttcattgaat aatttgactg ctggaacatt
     5341 acgggataag taaagtgctt ggcgtaaagt aatggtgccc atatattgat tatcccagtt
     5401 tcctacagga gtcgaagtcc cttcatagtt atacggtgcg tcagtaatag tttttccagt
     5461 agaatatttc aaatattcaa aagctggtcc gtaatctgtg acaggtttca tcgtagaacc
     5521 aaaatcacgg gaagtattca ctgctaagtt gttgcctaaa gttacgtctt ctgcgatgtg
     5581 acgtccgcca atctgggctt ttactttccc ggtattggtg tcgataaggg tggaggcaac
     5641 ttgcatctcg tcgtctgggt aagagacata ctgatccgtg ttgacgatat catatagttt
     5701 cttttgggcg tccagatcca aattcgtata aatctctaaa ccgtctgtgt agacattctt
     5761 atctgttttt tcttgtactt cattgatgac ttctttgaca taattatcga cgatcttagt
     5821 attgtcatcg gattgagtta gttcttgcag accatccgtt accggtacat taacagcttg
     5881 atcatattct gtttgagaga ttttttcgtt ttgaagcatc gtatacaaaa cagtatctcg
     5941 tcttttcttc gcctgatctg gataaacata aggatcatag gcggaagggg cttgaggcat
     6001 cccagcaagc aatgctgttt gcggtaaaga aagttctgat aatttcttac caaagtacat
     6061 ctcagaagct gtctccatcc cgtacaagcc gtttgacata tacactttat ttacgtagta
     6121 ggtcaagatc tcttgtttcg attttttttg ttccaatctt acagccatcc acgcttcttg
     6181 ggccttgcgt ttcagagtct gatcctcggc actggtggag aagaaggaaa gtttgatcaa
     6241 ttgctgtgtc aaagtacttc cgccttgcag tccgccagag gtaaagttag agagggcaga
     6301 accgatgatc cgaatagggt caaccccgat gtgtttataa aaacggcgat cttctaccga
     6361 cacgatcgca tcttctaatg ttttcggtaa ttcattcgca gaaatttttt cacgcttttc
     6421 tgcgcctaaa tcttcgaata gttcgccatc ttgtgtatat agtttggaag atacggtagc
     6481 gtctaatttt ttatccgtca attcgggcgc atcttttgcg taataccaga aaagtcctac
     6541 acctgctaaa aagaggatac agaaaaatga gagtattcct aaaaggatct tgataaataa
     6601 tcctttttta tgtgttccag aggactttcc agaacctttg ccagaattct tcttcacact
     6661 ctttttagga gtaggctgtt tctgtcgtct agagcttcta gtttgttcat ttgccattct
     6721 tgctttctcc ttcaatagat aatgttgata aatagaagtc aaccgcgtcc aaataaggaa
     6781 tccgcggaga aataccagtt ttgatctcta ttcctagttc ttcgatcaaa gataaaggta
     6841 gtgatttgcg tcctttttcc ttttgggcgt cccagtaagc aatcaaatga acactattca
     6901 aataaaaaca gcgcttcaaa gacgaaaacc ataacaagac aaagcagacc ccattttgtt
     6961 cgaggcatgc tttcatatgt tcgatttgat gggcatggaa gtttttaaag ggaaaagatg
     7021 ttttgttctt ggtttctttt gcttcaaagt ccaagtaaaa gcctcgatag acgccattat
     7081 agtctgtagt tgaggcttgg gtaaagtagg cttccttgat cacagcagca cttctcttag
     7141 gatagtctac tttcacgatt tggatagggg taggcttttt atggataacc gccaacccgc
     7201 gggaaagata atatttatta ctttcattga ttgcttcttc aaagcgcatc ccccgattgc
     7261 cgaattcagt gatctgattt cttttttctt gaattttttc gacgggagag gcattcttat
     7321 aataaggttg gccatttgga tagcgcaata ccatctttaa tcacactcct aggccacatt
     7381 atagcaaaaa agcggataga aagaaaagga atctgagatg gataagtcaa aagtaatcta
     7441 tgtgtctggg tatcgaagtt tcgaactcgg tgtttttaaa gaaaatgatc ctaaaatcca
     7501 agttgttaaa aatgttttaa aaaaagaact ccatcagtta gcagaagaag ggctggaatg
     7561 ggtacttgtc agtgggaatt tgggcgtcga actatgggct gtgcaaatag tagcggaatt
     7621 gaaagaagaa tatccggaac tatctcttgg tattttgtat cctttttctg attttggtaa
     7681 taactggaat gaagggaacc aagaaaaaaa acaattagca gaacaattag cagattatgt
     7741 cgaagctgtt agccatcaac cctaccaatc acctagtcag ctaaagaatc acactcgttt
     7801 tatacttgag catacagtgg gctgtctgct gatctatgat gaagaattcc ctggaaaaac
     7861 gaagtatttt tttgaagatg cacagaatta ccaagcagat catccctacg aaattcggct
     7921 gatcagtatg gacgatcttc aaaattttag tgaataatcg atattgtgtt tgatttatca
     7981 acttttttcc tatacaatga aaaaggtaga aatgttatta cgtaaatgaa aactagaggt
     8041 gtaaatatgg cgaatttggt ttatagtcct aaagacattt tacaacaaga attcaaaaca
     8101 aaaatgcgcg gctatgatcc cgttgaagtt gatgaattct tagacaatat catcaaagat
     8161 tatgaaactt acagcaaaga actccttgct ttacaagaag aaaacgatcg attgagtgca
     8221 aaagtagctc aattatctaa aactcaagga gcagctcaaa cacgcgtgca acaaacagag
     8281 gcaccaaaga gcgcagcagt gaccaatttt gatatcttaa aacgcttgtc aaatcttgaa
     8341 cgtgaagtat ttggtaaaaa gcttgatcaa caagcaagtg cagtcaaacc agcacaacct
     8401 aatccaaata actacacaaa tgcagacaca agcttagatg ataatgaaaa aacacgtcaa
     8461 ttttaatttt tgattaatga atttgcggtt ttcgggtaat cgcggtctga cttgatcagg
     8521 ctgaggaaag tccatgctcg cacaggctgt gatgcctgta gtgttcgtgc ttagcgaaac
     8581 cataagctaa ggtactcttt ggagtaacgg caggaaaaca agctaaggct tcggctatgc
     8641 ttaagtatcc ttgaaagtgc cacagtgacg aagcggctgg ggaaacccat ccggtggaac
     8701 gcggtaaacc cctcgagcga gcaacccaaa caatggtagg ggcgcttttt gtctggaaga
     8761 agaacaggac aaaaaggcag tataactgca gatagatggt taccgctttg ttttttctcc
     8821 ctggaaaagc aaagtacaga acatggctta tagaaaaccg caaacatcag ggaattaggt
     8881 cgtgacaaaa gtaatgcgtt ataagaaaca agaaaaacat ttgaaagcta tttttcaata
     8941 cttcagatga atcgttctta ttacaaaaca aacttttgta tcaccggtta ttcggaattt
     9001 gagtaaaagt gtggggtagg actgatgtcc cacctccttt ttttataaat aaggagattg
     9061 tgtatggaaa aaaacaggaa gttcaacctc gtcgcaactg cggcaagcgg gctagaagca
     9121 ctggttggta aagaattaag agatcttgga atcgaatgcc aagttgagaa cggacgtgca
     9181 cgttttgaag gaacaatgga aacgatcgct acagctaatt tatggttacg tacagcagat
     9241 cgagtaaaaa tcgtggttgg ggaattcgat gcgtatacgt ttgatgaatt gtttgaaaac
     9301 gtaaaagcat tgccttggga agattttctt ccactagatg cagcttttcc tgtagctgga
     9361 aaatcgatca agtcgaaact atatagcaca cctgattgcc aagcgatcac aaaaaaagca
     9421 atcgttgaac gtctccgtac ctattatcac cgtccagctt ctgtaccact gacggaaact
     9481 ggcgcacatt ttcagttgga agtagctttg ctgaaagacc atgtgatggt aactctggat
     9541 acgacaggac caagtttatt caaacgtggt tatcgcttag aaaaaggtgg agcaccattg
     9601 aaagaaaaca tggcagcagc acttattatg ttgacaaatt ggcgaaaaga ccgtccattt
     9661 tatgatcctg tatgtggttc aggtacgctt tgtattgaag cagccttaat aggccataat
     9721 atcgcaccag gattcaatcg tgagtttgct tgcgaatctt gggattggtt cagtcaagaa
     9781 atcatggaaa atgttcgatc agcagccgaa gaaaaagcag attatgatat tgaattagat
     9841 attactggtt cagatatcaa tggacggatg attgaaattg ccaaagccaa tgcagaagaa
     9901 atcggattag gctcttcgat tacctttaag caacaagcag tcaaagactt caaaacggat
     9961 aaagaatacg gggtcatcgt ggctaatccg ccttacggag aacgtctagg agaagaagaa
    10021 actgttcgga aattatataa agaaatgggc gatgtattcc gtccattaaa aacatggagt
    10081 aaatatatct tgactagcga cttagcgttt gaggaatttt acggacaaaa agcaaccaaa
    10141 aaaagaaaat tgtacaatgg tgcattgcgt acggatctat tccagtattg gggaactcgt
    10201 ccgcctagaa aaaaagagaa agacgaataa tcttactaat tgattcattc aattggatgt
    10261 ttggaggaat agggaatgaa agaaaaagag tttttacaag aagtaaaaga aatcaatctt
    10321 ctacaacaag ctttaggaat cctcgattgg gatacacaaa caggtatgcc tgaaaaagct
    10381 agtgcatacc gaagtgaagt agacagctat ctttacagca tttatttttc aaaaaaaatc
    10441 ggtccaaaaa tcaaagaagc cattcgctat ttttctgaac atccggaaga attgtcggaa
    10501 gttggaactg ccgtttttgc caaagtaaaa gaagagtatg atttggaaca tcgcgtaccg
    10561 gaagatctta tgagaaaata tacagctgcc gtttcttcag cacacggaca atggcaaaaa
    10621 gctagagaaa cacaacaatt ttctgatttt caacacgcat tgaatcaaaa tattgaatta
    10681 acaaaacagt tgatacctta ctggagaaaa gatgaaaaga cgaattatga tgttttgctg
    10741 aatcaatacg aacccgggat gactgtcgaa atccttgata aggtatttga tcagcttaaa
    10801 gaaggaattc tttcgatccg ccgtacgctc caagaaaaag ggactgaacc tgaaacagac
    10861 tttttaaatc ggagaatgac gaaaaaacaa cagaagagat ttgtagtgaa agtaatcgaa
    10921 caacttggtt atgattttag tcgtggtcgt ttagatgaca ctgtccaccc atttatgact
    10981 ggaattaatc cgaatgatgc acgaatcacg acacgatgga acgaaacaga tttcaaaatg
    11041 gctgtttttg gagtgatcca cgaggctggt catggtatgt atgaacaaga catcgatgat
    11101 aaatatgcgt atacaccggt ttatgaaggt acatcaatgg gtatccatga atcacaatcg
    11161 ttgtttaacg agatcattat tggaagtagt cgcagcttct ggaaaaagca atatcctttc
    11221 tttcaagaat gtgcagaagg aacattcgat gatattcctt ttgacaaatt ttatcgttca
    11281 ttgaaccata caaaatcaag tttggttcgt atcgaggcag acagtttgac ttaccctctc
    11341 catatcatca ttcgttatga aatcgaaaaa atgattttca atgaaggggt agatgttgct
    11401 gaactacctg aaatatggaa tcaaaaatat gaagagtatc tgggaatacg accaacaaat
    11461 gatgtggaag gcatcctaca agacgttcat tggtcaggtg caagctttgg ttattttccc
    11521 tcttatgcat tagggtacat gtatgcggca caattacttc attctttgca gcaagaaata
    11581 gatgtggaaa atgttctagc gtcagacgat tattctccaa tcaaaaactg gatgtcagag
    11641 catatccatc aatatggtgc atctagaaaa ccaaatcaat taatcatgga tgcaacacat
    11701 gaaccgctta atccgcaata cctgatcgac tatatgaaag acctgtattt taatgtatat
    11761 caggtaaaat aaaatacaat ttacaaagat agctgaaatt catctaatct atagatgttt
    11821 tcagctattt acttaagaaa gaagtggaac gaatggataa attacaagtg atcgctgatt
    11881 atagtatgga ccaattagcg catgatcaaa caggacatgg gagcgatcat accaaaagag
    11941 ttgtcaagtt agctgaaaaa attttggaga cggaaccgca ggcagaccgc tttgtgacgc
    12001 ttgcagcagc ttacctacat gatacgattg atgataaagt cgttaaaaat gagaacgagg
    12061 caaagcaaca gcttcgtgta ttccttcgct ctttgccaat agtagaggaa cagatctcga
    12121 tgattttttc cattatcgaa aatatgtcct tttctaaaaa tctctctgaa gcggccgaac
    12181 tatctctgga aggtaagatc gttcaagacg ctgatcgtat cgaagcatta ggagcaatcg
    12241 gcattttgcg tactgcttat ttcggcggtg gacatgggca cccgattttt gatcctgaac
    12301 tgtatccgca aacattcaag gacaaagaaa attaccgaaa aggcacgaca gtgatcaatc
    12361 atttttatga aaaactgttc ttgctgtctg ataaaatgaa tactgattac ggatatgaag
    12421 aagccaaacg aagagaaacc ttcatgcgtg aattcttaga agaatttttt ctggaatggt
    12481 ctattgacga tgaaaatttt actccagaca attggatcaa aaataacaaa aaatgatgga
    12541 ggttgtgaca gaagtggtca gctccaagat atttcggatc attttccgta aaagttactt
    12601 ctggaacact gtttaccaag aggtcgtgag cctgccgttc agctccgagt cacaaggaac
    12661 aatttcaaaa aatagcttct cgtgtttgat gaatattgta acttgtgatt cggaattggc
    12721 aggagaacac cgtttatt
//
LOCUS       DAZBSA010000085        12734 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000085 DAZBSA010000000
VERSION     DAZBSA010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.085"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(87..266)
                     /gene="rpmF"
                     /locus_tag="ACHGMT_002154"
     CDS             complement(87..266)
                     /gene="rpmF"
                     /locus_tag="ACHGMT_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFN6835881.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(324..893)
                     /locus_tag="ACHGMT_002155"
     CDS             complement(324..893)
                     /locus_tag="ACHGMT_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFN6835882.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1079..1981
                     /locus_tag="ACHGMT_002156"
     CDS             1079..1981
                     /locus_tag="ACHGMT_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFN6835883.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2016..3800)
                     /gene="pyk"
                     /locus_tag="ACHGMT_002157"
     CDS             complement(2016..3800)
                     /gene="pyk"
                     /locus_tag="ACHGMT_002157"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFN6835884.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3874..4836)
                     /gene="pfkA"
                     /locus_tag="ACHGMT_002158"
     CDS             complement(3874..4836)
                     /gene="pfkA"
                     /locus_tag="ACHGMT_002158"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFN6835885.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5004..8318)
                     /gene="dnaE"
                     /locus_tag="ACHGMT_002159"
     CDS             complement(5004..8318)
                     /gene="dnaE"
                     /locus_tag="ACHGMT_002159"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFN6835886.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8549..8734
                     /locus_tag="ACHGMT_002160"
     CDS             8549..8734
                     /locus_tag="ACHGMT_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFN6835887.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8796..9992)
                     /locus_tag="ACHGMT_002161"
     CDS             complement(8796..9992)
                     /locus_tag="ACHGMT_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFN6835888.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10162..11598)
                     /locus_tag="ACHGMT_002162"
     CDS             complement(10162..11598)
                     /locus_tag="ACHGMT_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFN6835889.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11891..12700
                     /locus_tag="ACHGMT_002163"
     CDS             11891..12700
                     /locus_tag="ACHGMT_002163"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN6835890.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3802 a         2672 c         2250 g         4010 t
ORIGIN      
        1 atatttttta tttaccgact acaaaaaaat aaacctcaag gctccatggt tagccttgag
       61 gttttacatt ttctaattaa aatgtattaa gcttcttttg acattacgtc cttaccatca
      121 tagtaaccac aatttgcaca tacgtgatgg ctttttttca tttcaccaca gtttggacat
      181 tcgtttaacc ctttgattgt caatttgtag tgtgtacgac gttttgcttt tttagctttt
      241 gatgttcttc tagctggtac tgccattttg ctacacctcc ttgtaaagtc ttgtatctaa
      301 attagacaca atatattcca atcttacttg ttatcgtcgt cttttgattt agaatcaaaa
      361 agttcagata gttttgcaag acgaggatca attttattct cggcttcttc catttttctg
      421 cgctggtatt cttcttcgga aacaacttcc caatcattgc cttttggaag ttctgttgct
      481 gcttcctctt cttttgtcaa tacccgcatt ggaatcgcca ataagatgtt gtcttcaatc
      541 gactctgcta aattcaataa tggaccttca attagcaaaa tctcatctgc agacatttgt
      601 tcatctctac tttggaattg ttcttctgtc atgaatactt cgtctacaga aaaatccatg
      661 ggcagttcaa caggttctag tgaacgtgtt gaaggaactg tcaaagtcgt ctgcacagtg
      721 taatgaacga tatagccttt cttatcaact gagaccaaac cttcgacttc cactggcgca
      781 acatcaagta ttaaattgtc tcgcttcatg agagcggctt tcaagtcgag cgtctcatga
      841 aaagttaacg gtgtctcctg gtattttctc aattccaata aagaccattt catttttcat
      901 cactcctaag caacaaaagc aattatacag ggcaaaaagc atgttgtcaa tgaaattttc
      961 ttgacacctt gcagttcata aaaaaacaga aaaatcttta ttagaaagcc tctgtttgct
     1021 atcatcctca gaatcattta caataatcat tattccatta aaggagcgga tgatcttcat
     1081 gaacacgaaa gatatcgaat actatgtcca aatcgtcaaa caaaaaaatt ttacgaaagt
     1141 ggctaattat ttccgtgtga gccaacctac tattacatat gcattacaac gtcttgaaaa
     1201 agagtttgac accgtactta ttacaagaga tcgatcgcat cacgaattaa tcgtcacgcc
     1261 ttctggaaag cagctgtttc tccatgcaca aaccattctc caagagattt ctactgcaag
     1321 agctgaaatc actcagttgc aaaagaaaaa gcttcgtttt ggcatgcctc ctattatcgg
     1381 caatgaatac tttcctaaac tgtcaagtta ctttatcaag catgaattga tgaatcaaat
     1441 tgagattacc gatggaggct ctcgggactt atacgggatg atccgacaag ggaggatcga
     1501 tttagctgta ttaggctcaa cacagccaat cgtggatcaa tatttagaaa cagagttact
     1561 tttggacaaa cgtttcatga tcgtcgtgcc acctacacat cctctttcca agcaggatac
     1621 tgtctctttc aaagatatag cagatgaaca gtttgtacta ttgaacgaac attatgtcca
     1681 ccccgcagca ttcactaagc tttctaaaca aacacatact catccaaaag ttgtttatca
     1741 aagcaatgac ttgacgattt taaagagtat ggtccgagaa aatgtgggta tcggtttttt
     1801 ggcagagatt gctgtccatc ctgaagatca tttagtttgc ctccctcttt cagatgaagt
     1861 tcagcctcgc ttcttgattt cattggcaaa acgaacacag cagatcgtca cgcctcttca
     1921 agaacaagta gtccaagtta tccatgaatt cgttacagca caaaaaaaca gctcttcgta
     1981 aaagaagagc tgtttttatt ttttattaag ctaaattaga tagctgttgt ttcgccacgg
     2041 taaacgatgc cgcgacgtga atcaacggta atcaattcac cttctgtaat tgttgttgtt
     2101 gcgcctttag caccaacgat tacaggaata tcttgtgcaa ttcctacaac tgcagcgtga
     2161 gaagtcaatc cgccttcttc aacgataaca gcagctgctt tttcgattgc aggcatatat
     2221 tctttgtcag ttgatggtac aactagaacc attccgtctt ttgctttttt tacagcttct
     2281 tcagcagatg aagcaataac tgcatttgca acgacagatt tttgtccaac gccttgagct
     2341 tctaataatt tagaaccaat catttgaatt ttcatcacgt tagttgttcc gcgttcacca
     2401 actggtacac cagcagtgat caagattaaa tcgccttctg atgcgaaacc taattcaaca
     2461 gcttttttag ctgctagttc aaacatttca tcagttgttg atggtttttc agcaactgtt
     2521 gggtaaacgc cccagtttaa catcaaacca cgttttgtgc gttcgtcaaa agtaacagcc
     2581 aagatatctg catctggacg atattttgaa atcatttttg cagtgtatcc tgattcagtt
     2641 gcagcaacga ttgttttcac gcctagattt tttgcagcac gagctacaga taaaccgatt
     2701 gtttcagtta catctgtttt atcgaattcg ttcaattgga atgtacctaa ttctgataat
     2761 gcattttcag cttcttggtc gatacgtgcc attgttgaaa cagcttcaac tgggtagtcc
     2821 ccgtttgcag attcaccaga tagcattgtt gcatctgtgc catcaaatac agcgttagca
     2881 acgtcagaag cttccgcacg tgttggacgt gggttttctt gcattgattc taacatttgt
     2941 gtagcagtga taactgggat accagcagca ttacattttt tgataatgcg tttttgaacc
     3001 attggtacta gttcagctgg gatttcaaca cccatatctc cgcgggcaat cataacaccg
     3061 tcagaaactt tgatgatttc gtcaatgttg tcgatacctt cttgagattc gattttaggg
     3121 aagatttgaa catgagtcat gtctttttct tctaagattt cgcggatatc tagaacgtct
     3181 tgtgctttac gtacaaaact tgcagcgata aagtcgatat cgttgtctaa accaaaacga
     3241 atatcgtcag catcttttgg agtaatacct ggcaaactaa tagaaacgcc tggtgcattt
     3301 acccctttac gtgatcctag catacctgca tttttaactt ctacgactaa ttcacggttt
     3361 gcttcgtctt tttcaatgat ttgcatatcg atcaaaccat catcaaataa aatgtgtccg
     3421 ccaacgtgaa catcgtcaaa taatcctggg taagtaaccg cgattttttc ttttgaacct
     3481 acatgttcag gatccataga gatgcgtgtt tgatctccta cttcaaattt gatgtatcct
     3541 gcacgaccaa aatcagcttc tgttgtgcct tgaacagttg tacggatttc agctcctttt
     3601 gtatctaaaa gaataccgac atcttttcct gtctttttaa ctgcttcacg gaccatagtc
     3661 atacgtgata attgttcttc atggtcacca tgagaaaaat taaagcggaa gacatttgcc
     3721 ccagattcaa tcaattgaga aataatttct actgagttac ttgctggtcc taatgtacta
     3781 acgattttgg tttttttcat ttaaaaaact ctcccattca attttgttta tttaaagctg
     3841 tacacagctt aaatagcttg gtttctttca ctcttaaaat gaaagtgaat tatttagatc
     3901 atatagtgat aagtctggtt tatgtttgtt gttttcaagt gtatcaatga tgtcagacgc
     3961 aacgatttcg ttgtcatgga taccgacaca taatccacct tttccttctt gaagcaattc
     4021 gacagcgtaa gcaccgaatt tacttgccaa cacgcggtcg cgtgcgcttg gtgctccacc
     4081 acggacaacg tgtcccaaga tagatacacg tgtatgatag tcaccaaatt ctgaaagttt
     4141 ctcagcgaat tcatttccgc ccattacacc ttcagccaag atgatcaagc aatgtttctt
     4201 cccgcgatcg cggccttctt ggattttttt agcaactgaa gccatatcaa aatcatgttc
     4261 tgggatgatg atttcatccg ctccgccagc cacacctgac cataaagcga tgtctcctgc
     4321 attgcgtccc attacttcga taacgaatgt acgaacatga gaagtcgctg tgtcacgaat
     4381 acggtcaatt gattctagta ctgtattgat tgctgtatca aaaccgattg tgaaatcagt
     4441 cccaggaata tcattatcga ttgttccagg aatacctact gctgggaaac cgcgttttgt
     4501 caaagccatt gcgccatgat aagagccgtc tcctccgata accactagac cttcgattcc
     4561 gaattttttc aattgttcga ttccttttaa ttgaccttct tcagttgcaa attctggata
     4621 acgtgcagaa tacaagaatg taccaccacg ttggatcttg tcacctacat ctgccacatc
     4681 aagacgacga atatctccag caactaaacc ggcaaaacca taattgatcc cgtatacttc
     4741 cagtccttcg taaatacctt tacgaacaac ggcacgaaca gctgcattca ttccaggtgc
     4801 gtctccgcca ctggtcaaaa ttccgatgcg tttcataata aacattcacc tcattaaaat
     4861 actttatcct aagtatgact taggtttaca accatttttg cgttaaccac gactaacatt
     4921 ctaacattat ttttctcact tgtcttgatt tacggataaa aaaatgaaaa aaaatgaaag
     4981 caagtagtat tttttaccgt aagttatttg aaaaccacat tatcttcgcc aagcagataa
     5041 ataagttgcc cctttatact cttgctgtca tccacccaat aagcatctga cagaactact
     5101 tttcgttgcg ttttttcgtg atacataact actggtatat atcctgggtg tttctgaaaa
     5161 atcacttgta attgtttcat tatctcggga tcttcccgtt cttctgtaat acgcaaatag
     5221 caagtttgtg aaggatactg ggcttctaac tggttaggat cagcaaagct ttctccaatt
     5281 agttgcagtt cctgattata tttgctgatt tcgctccgcc cttgaacaaa gatcactcga
     5341 tcaacggcaa acaaattacg atatttacga taaagagttg gaaaaactgt gacagaaatt
     5401 tctccactac tgtcggttcc ttcaacaaag gccatcagtt ctcctttttt tgtccgaatt
     5461 tctctaactt ccttgatcat cactaataaa ttgactggtt gattagggac gatatctgta
     5521 atcagctgaa cttgtttagc taaacgaaga cgatcaaacc cttctgcagg atgacctgac
     5581 aaataaatac ctaagtactc ctcttccagc ttcaagcgtt cttctagtgt atagtcagcg
     5641 acttcttctt cttttaatgc catgattccc agcaagtcaa gacttccacc actgtaaacg
     5701 atattttgga tttttccttc gagatcaagc atcaattgtt tcctgttagg agataattca
     5761 tcaaaaactc ctactgctgt cagtgattgc agtaactcta atttcagcca acgcttatcg
     5821 atccgtatga ggaattgatc cactgactgg aacgggccat tttgttttct ttcgtttaat
     5881 atatcttcta caaaatcacg ccgaatacct tttatcgctg tcagtccgaa tctgatttct
     5941 tccttattga ccaactcaaa actggaatta ctttgattaa tggacggcga ggaaagtttg
     6001 agtttatttt tacgtgcttc tgcaatatat tctttcaatt ttgatgtgtt atgccgaaca
     6061 gaattcatca ggctggtaaa gaaagcacct ggatgatggg cttttagata cgccatttgg
     6121 aatcccacga atgaataagc aaatgcatgt gaccgattga atccatagtt tgcaaatttt
     6181 tcgatatatt catatacggt cgctgctgtt ttttccgaat atccctgttg taatgctcct
     6241 gatacaaaat gcgacttttc ttcatctaat acgtcttttt tctttttact cactgcccgt
     6301 cgcagaatat ctgactgccc taatgtaaat ccagccatag tcgaggcgat ctgcatgatt
     6361 tgttcctgat agacgatcac tccatatgtg tttttcaaaa tcgggatcag tgaatcatct
     6421 gggtagcgaa tggcttcttt tcctttcttc ctggcaatga aggtatctat attttgcata
     6481 gggcctggtc gatacaatgc attgactgcg gcaatatctt cgatgttttc tggatctaat
     6541 cttcgtaaaa cgttccggat accagcagat tcaaattgaa atactcctga ggtttcgcct
     6601 cgttgaaaca agagcagcgt ttttgcatca tttagcggga tctcttttaa tcggatctca
     6661 tacccttcta ctcgcttaac agctttcaaa gtaaaatcaa taatcgataa gttgcgtagt
     6721 cctaaaaagt ccattttcaa aagaccgatt gcttctacct cattcatcgt atactgggtc
     6781 agaaaagctt catcagaacc gcgttggagc ggaactagtt caagtagatt cttatcgctg
     6841 ataactacac ctgctgcgtg agtcgaaaca tgtcttggca atccttctaa aatcactgca
     6901 gttttgaata aaagttggtt tttttcgctt gcctgaacta attctactaa ttgatgcgaa
     6961 gttttataag cttgggtcaa tgtgattttc aattgattgg gtattgcttt tgaccagcgg
     7021 tttgcttcac tttgcgataa accaaacact ctagctgcat ctctaagaac catttttgct
     7081 gccattgtgc caaatgttgc gatttgtgcc atatgatatt gtccatactt ttctcgaaca
     7141 taattcaata cttcttcacg tcgattgtca ggaatatcca aatcgatatc tggcatcgta
     7201 aagcgttcgg ggtttaaaaa gcgttcgaac agcaaatcat attctaatgg atccacatct
     7261 gtgatttcta acacataggc tactaatgaa cccgcagcag aaccacgacc tgcaccggta
     7321 acgattttgt ttctatgagc aaaagccatt acatcccata cgatcaagaa ataatcatcg
     7381 aatcccattt gatggatgac agataactcg tagtccaaac gttcttggta ttcctttgta
     7441 tagtcagtga ttcggcgttc cacttgcgaa tagcataact ctcttaaata ttctccagct
     7501 tgttttccat taggaactgg ataatgcgga agtaatttct gatggaactg gaattcaaat
     7561 gaaatcgatt cagctaaacg ctgggcgcct attacagctt gggaaagacc cgctttttcg
     7621 aatcgtttga tcatttcttg ttctgaaaga agatagacat ttgctgttag tggtaaagat
     7681 gcagataaat tttctaaagg tatttggctt ccctctttga tatgtttcat cacttcaagt
     7741 gcaaaaagtt cttcttgttg taaactagcg atgggatgta atgcacaaag cgaataattt
     7801 tcttttatca catactgata ccattcaggc aattgatcag gcgtgttttc tgtcaagccg
     7861 atataaaaat ttctagtaaa aacattttca agtaaaaact gccaatgacc gaaaaagtta
     7921 tcctctttat tgaaagcctc agaaacagaa tgatttgccg gaacaacaaa cgctagatgg
     7981 tcggtttctg tcatttctgc aagttccaat ttttcttgga tcatttttcg gctggaaagt
     8041 ttcatcagct gctgataacc tatttcatct tttgcgtacg caagcaagcc ataatttttt
     8101 tcttcataag tataactaag atgtaatcca atgattggct gtactccttc ttctacacaa
     8161 atacgataga attcaggtgc accgtataag acatctttat ccgttatggc taatgaagtg
     8221 tagccttgtt tttttgcttc ttgtacatat tctcgaatac gtatcgtact ttgtaaaaga
     8281 gaataagaag taattgtata aagttgtggg aaaaacatct tgcattccgc cttttttttc
     8341 gttaaaattc actagcgtta aaaggtttga tccttttact ttcattaaga tagcaggcgc
     8401 tgaagttgat tattccactt catttgcttg cttgtaatct gtaggaatga gttctttcgc
     8461 aagaactcaa attttattga gatttggtct cgtttcttta cattcaaaga aaatctggta
     8521 aaattaagat agaaaagagg tgttctttat gcgatatatc gttacattat tctgggctgt
     8581 cgttctaggt caagtagtcg gttatattgg tgctgcatta acaagcggaa catatgattt
     8641 tactttgaca acaatcattt cattcattgc gggtgtcatt atccttttga ttggtgcagt
     8701 tgcgccaaga aaagaaacct ctgcccattc gtaaaaaaac agccaagagc ttgagatttc
     8761 tcaagctctt ttttattttc tactcctcac caccattata atctgctgcg aagaacttta
     8821 cgaaagttga ataaagaaag aagaatattt gacagaacaa acaaacttgt cacaataaaa
     8881 acagcactgt aattataagc atgcgcgact gtcgatccta gcatgggacc taaaacttgt
     8941 ccaaagttgc taaacatctg attgtagcta tagattcggc tgactccttc ttgaggcgta
     9001 atcttactga tcagtgtatt gacagaaggc atcaaagcgc cagttgaaaa gcctaaaata
     9061 aagcgaagta tacctaattg gagcggtgta gtgacgaatg ccataggtaa ataacagcaa
     9121 aatgataaaa tgagtcctcc aattagaact ttttgattcc caatcttatc ccctagtttt
     9181 cctagtttcg gtgaagaaaa gacagcggaa acaccagcta tcgaaacaat caagccgctg
     9241 acaaaaagga tattttctgt acttccgctc aattcacgaa tatataaggt caaaataggg
     9301 ctgatcgtag tgattcctat ctgtaaaatc aaggtcgtaa tgaagagacc gatcaaaata
     9361 gagagatgat ccatttttga aaaaatttct ttggtgctta tcagatcttt tttctcgatt
     9421 ggatggaaat cttctttgac taagaaaatg gtcaataccg ttgtgatcat caacaaagcc
     9481 cctgtgatca agaatacatt ttccatacca aaccactgag ccagtgcacc gcccattgaa
     9541 ggtccgatca gatttccagc aatcgctccg gtagacaatg ttcctaacgc ccatccgctc
     9601 ttttctctag gtgcctgtga agcaatcatc gctgtggcat ttggaatata tccagacaaa
     9661 atcccattcc aaaaccgcat gatcaaaagc caatacgcgt ttggtacaaa agccaaagat
     9721 cccattgtta atgtcatccc tgctgctgca cggatcatca tgagttttcg gccttttcga
     9781 tcagctagat ttccccagat tggtgcaacc acggcagatg caaaagcagt cacagagatt
     9841 gctagtcccg aaaacaattc gatttgatct tttggtgttc ctagttgctc aacataaact
     9901 ggtatgaatg gcataactaa actaatactt gcaccagtga aaaaacagcc aatccacgca
     9961 atcactaaat tttttctcca attaatctcc atctatcatc cctctttcgt ctcttttatc
    10021 atacctattt tcgaataaac aagcaacgat ctcgccagta aaaagagaaa aatcccgcaa
    10081 aggtcatttt atatgtaacg ttctttttat gtaaaacaaa aagagatcag aacaaatact
    10141 gttccaatcc cttctcttta gttaaattgt tgctagaatg atgaagttga taatgaataa
    10201 aatatcaacg atccacaaag caacagaaac ttgattgtat tttcctttga ttactttcac
    10261 aattgtatag aaaataaatc ctgctgcaat cccataggaa atgctatagc acaatcccat
    10321 aaagatcgat gcaaagaagg ccggtaaagc ttcttccata tctagccatt tgatgtctgc
    10381 aaaagaagcc atcatcatta ctccaactaa aatcaatgct ggcgcagttg cttgatttgg
    10441 tacaatggcg atcagtggag agaataaact acttaatgcg aatagcactg ccactactac
    10501 tgaagtcaaa ccagtacgtc caccggcccc gatcccagct gcagattcga cataagttgt
    10561 tgtattcgat gtaccaaaga tagcccccac agaagtagcg atcgcatcgg caaatagtgc
    10621 tttatccatt tttgttttga acccacgacc atcttccaac gcgatctcgt cttcttttga
    10681 gaaaatacct gtgcggcgtc ctgtaccgat aaatgttccg atcgtatcga aagtatcaga
    10741 taaactgaat gcgatgatcg tcatcaatac ttgaggaatc ttggaagaat cactgaataa
    10801 agattgcatg ccttcagaac caaatgctgc accgaaagtt gttcctaatt ctttaaatga
    10861 attacctaac gaattcgttt gccaatcaat ggaacttaaa tcaacaacac ccataaaaat
    10921 accaaggact gtggtcgcta cgataccgat aagaatagcc ccacggacat tttttacaac
    10981 taaaatggct gtcagtacca aaccaatcac agccaataag ataggagcgt tgtcaaagtt
    11041 ggctaacgca gggacgattc caccatttat cgtaacattt gtcgcttttc cgccttcaac
    11101 gacagagctc gaaatagctg actggtctgc tgaaaaagat aagaatcctg catttttgat
    11161 tccgacatac gcaacgaaaa taccgatccc gccaccaatc gcatgttgaa gactttcggg
    11221 aatcgcccga ataatcattt ttcgaatatt tgtgacagta ataaaaatat tgattaatcc
    11281 gcaaataaac accattgcca aggcttgttg ccaagaataa ccaagcccaa aaacgaccgt
    11341 aaaagtgaaa aatgcgttca atcccatacc aggtgcctga gcatatggta catttgcgaa
    11401 caatcccatt accagcgtac caataatcga tgcaatgatt gtcgctagaa atactgcttg
    11461 gaagggcata cctgatgctg acaaaatcgt cggatttacg aataagatat aactcatggc
    11521 aaagaatgtc gttactcccg ccattatttc agttgataca tttgtattgt ttttctttaa
    11581 accgaaaaac ttttccataa aaagttccac tccctgaatt tttttctgat ggaaaactat
    11641 aataataaaa aataagtaaa atagggcaaa tccatcgaac aatttcaaat tatatagacg
    11701 tttcattcgc ttttcaacgt ttttttaaca aaaaaagcga aaataatact taacgttcgt
    11761 gtttagctta tgtttagcta tttatatagc cataactgta gtttttgtta ggaatagctg
    11821 attgctcttg ttaattacct aaaatatgct agactaaaac aaatgctagt tgaggaagga
    11881 aggaccctta ttgaataaaa aattaattgc catcgatctg gacggaacta ccctcaatca
    11941 agattcgatg attacatcta aaacaaaaga gactctaaaa aaagcaatca atgccggtca
    12001 tcatgtcagt attgctacag gacgcccatt ccgaatgagc catcagtttt accaacaatt
    12061 agaattaaca acaccgatgg ttaattttaa cggcgcatta gtacacatcc ctaatcagca
    12121 ctgggatggt gaaagagaaa cgttgatcaa tcgtgaaatc gcttttgaaa tcttatcgca
    12181 aaaaaagcag ctaaatttag attttatcgc tgctgaaaat cgtgatactt tttttattga
    12241 cagttttgat ttctttgatg aaaaaatttt tgcttcttct cgtccaggag agaagaattt
    12301 gctttctcct aaaaatttga cgacaaatcc tacatctttg cttgtccgaa cagacaaacg
    12361 ttttgcagga gctgtatctg ctgaattaac acgtcaattt ggcagttatg tcgatgttcg
    12421 gacatggggt ggacctactg ccatcttaga gattgtttca aaaggaatcc aaaaagcaaa
    12481 aggcgtgcaa gagattgcta actacctgtc tatcgatcaa aaagacgtca ttgcctttgg
    12541 tgacgagcat aatgatttgg aattgttgga ttatgcaggt tggggagtag ctatggctaa
    12601 cggtactgac caactcaaag gcattgcaaa tgatgtcact cctttgagca atcaggaaga
    12661 cggtttagct gtttatctgg aaaaactact taaactataa tagagaatta gattccacaa
    12721 aaaagaggtt gtga
//
LOCUS       DAZBSA010000086        12188 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000086 DAZBSA010000000
VERSION     DAZBSA010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.086"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..314)
                     /locus_tag="ACHGMT_002164"
     CDS             complement(150..314)
                     /locus_tag="ACHGMT_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835891.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            609..1937
                     /locus_tag="ACHGMT_002165"
     CDS             609..1937
                     /locus_tag="ACHGMT_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFN6835892.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2139..2972
                     /locus_tag="ACHGMT_002166"
     CDS             2139..2972
                     /locus_tag="ACHGMT_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFN6835893.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2988..3725
                     /locus_tag="ACHGMT_002167"
     CDS             2988..3725
                     /locus_tag="ACHGMT_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFN6835894.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3880..4848)
                     /locus_tag="ACHGMT_002168"
     CDS             complement(3880..4848)
                     /locus_tag="ACHGMT_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFN6835895.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5080..5913
                     /locus_tag="ACHGMT_002169"
     CDS             5080..5913
                     /locus_tag="ACHGMT_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFN6835896.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5947..6786
                     /locus_tag="ACHGMT_002170"
     CDS             5947..6786
                     /locus_tag="ACHGMT_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN6835897.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6812..7372
                     /locus_tag="ACHGMT_002171"
     CDS             6812..7372
                     /locus_tag="ACHGMT_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN6835898.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7571..9292
                     /locus_tag="ACHGMT_002172"
     CDS             7571..9292
                     /locus_tag="ACHGMT_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFN6835899.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9457..10599
                     /locus_tag="ACHGMT_002173"
     CDS             9457..10599
                     /locus_tag="ACHGMT_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFN6835900.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10615..11826
                     /gene="thiI"
                     /locus_tag="ACHGMT_002174"
     CDS             10615..11826
                     /gene="thiI"
                     /locus_tag="ACHGMT_002174"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFN6835901.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4153 a         2046 c         2531 g         3458 t
ORIGIN      
        1 ctactgccgg agtaaataaa gcttatgcaa agaaaaagaa atccaatcac ttgggtttct
       61 ttttctttta ttattatgat gaatcaagct ttctgttttt gtcacgtatg ttgtaatctt
      121 tacgaaagag tagagttggc tactcataat caaccatact tcccatcatc gctaaatacg
      181 aaataaatca gcattcctac accaatcaaa gcacaagctg caccaagaaa agggatgaca
      241 ttcccaaagt tatctgtatt cataagtatg ccaccaccca caaacaaaaa gccgccagca
      301 atcaggtttt tcattttatt catctccttt cttacgttat agtatttcct tctttcaatg
      361 agtattttga aagctatctt attgtatgat gttcttctag gcctcttctt tattatacag
      421 gccattttcg ttatctcata gaaatattcc tttttatctg aaaactattg tataccgtat
      481 gacatttttc ttcatgaagt tgtaagtata ttctcattca aatgaaaatt tcatcataga
      541 agagattgta tttctaaatt cttaagagta aaatacgaag tataacttgg atttggaagg
      601 atgattggat gaaagctatt acgcctgata tgacaacaaa atttaacaaa cagtttcaag
      661 caaatatcaa acagcaggca ttgcagcggg cagttgtgaa gaacggaatt actgcttctg
      721 ctgaaaatgt ttcagcacat gtggagaata caccggtatt ttctatagat ttggctacgg
      781 ggaaagtggc taatcaaatg caatcggggc gttgctggat gtttgctgca ctgaacacat
      841 tccgtcataa gatgctgaat caattccatt taaaagagtt tgaattatca caaaattaca
      901 cttttttctg ggataaatat gaaaaggcaa attatttcta tgaaaacatc attgcaacag
      961 gaaaagaggc aatcgatagc cggaaagtag ctttcttact cgctactcct caacaagatg
     1021 gcggccaatg ggatatgatc gtttcattat tccaaaaata tggcgttgtg cctaaatcgg
     1081 tgatgccgga aagtgcgaat agttcaaatt ctcgagattt gaataactac ttgaacaaat
     1141 tactgcgtaa agatgcagta gtgttacgga aaatggttgc tcaaggagaa acattagacg
     1201 ctattgaaga aaagaaagaa gaaatgctag aaagcattta taacttctta gctatctcat
     1261 taggaacacc tccaaaagaa ttcgattttg agtaccgcga tgaagaaaag aactatcatc
     1321 ttgaccgagg actgacacca caaatttttt atgataaata catcggtgta aaattagatg
     1381 attatgttag cgtgatcaat gcaccaacaa aagacaaacc attcaatcgt tcttatacag
     1441 tagagatgct aggaaatgtc gttggcggga aagaagtcaa atatctgaat gttgatatgg
     1501 aaactttcaa aaaattagcg attgctcagc ttgaagaagg agaatctgtt tggtttggtt
     1561 gtgatgtagg tcaatcttct actagaacta gtggcatcat ggccttagat gcttattcaa
     1621 tggatgatct atttgatacg gattttacaa tgactaaagc tgaacgttta gattttggag
     1681 aaagtctgat gacccatgcg atggtattga ccggtgttga cttagtagac gggcaatcaa
     1741 caaaatggaa agtcgaaaat agctggggcg aaaaagttgg aaagaatggc ttctttgtta
     1801 tgagtgatgc ctggatggac gaatatacgt accaaatcgt tgtaagaaaa gatttattga
     1861 cgaaagagca attaaatgcg ttcaatgaag aaccaattgt tctatctccg tgggatccaa
     1921 tgggtgcttt agcatagacg agtatttcaa tgaagagtat gtagtaaaga taaaaagagc
     1981 tggcttaagc cagctctttt tatctttaaa gtccattccc tgttttttat ggtccctgtg
     2041 ctataattct aaataaagga taaggattgg aaataatcct tttatcatta aaaatatgat
     2101 gacgaagtag aataaaattg ggagtagggg ggaaagtcat ggatatccaa actgttttat
     2161 ctgatctgaa attaaatggt aaagtgattc cagttgttgg tggcgatgtt aatcagacct
     2221 atcgtataaa aaccgaacat cgtgcttatt ttttaaaaat ccatccgaat gtaaaaaaag
     2281 gattttttga agcagaagta gacggattaa aagaactgtc agcattcgta cgtgtaccgg
     2341 atacgtatat gctaggtgaa acaagtgaag gcgcttattt gttaatggaa tggatcgagc
     2401 cgggaaaagg ggatcaacga gatcttgctg cagctttagc taatctccat cagcaaacag
     2461 cgccacaatt tggttttcgt aaagataact atttgggtac acttgttcag aaaaattctt
     2521 ttgaagaaga ttggtggact tttttcttta aagatcggct tgaatcccag atttctttgg
     2581 cagaagaaac gaatcgatgg aatgtacagc gtcaagaaaa gtatctgcga tttaaagaac
     2641 gtgtattgaa atcggttgaa ccaaaaaaga tcactccacg cctcttgcac ggagacctgt
     2701 ggagtggtaa tgtgttcttt gaccagcaag ggcatccagt ttttgtcgac ccagctgttt
     2761 cttatgggaa tcgggaacaa gatattgcaa tgagccagtt attcggtggt ttccgaccgg
     2821 aatttttaga tgcttatcaa actatatttc cattagaaaa gggatggaaa gaccgactgc
     2881 cgatttacca gttatattat cttttggcac atttgaatat gtttggagaa agttacggtt
     2941 cccaagtaga tcaattacta gaaaattttt aagaggaggg cgtgtttttg gcaaaaaaat
     3001 ataccaaaaa gcatgaagtt tcatattatg agtgtgacat caaccaaacg atgacatttc
     3061 cttcgatgtt aggagtagtt atcaaaacgt ctgaagatca aagcgaccag ttgggaagag
     3121 gcagtgaatt cgtcaatagc tttggactta cttgggtcat cacgaattat tccatggaga
     3181 ttaatcgtct ccctagagtc ggtgaagtaa tctcagtaac gactcaagcc atggagtaca
     3241 ataagttttt ctgttatcgg aacttctgga tccatgatga agatgggaaa gaattagtca
     3301 aaatagaatc tgttttcgta ttgatggatt ttgtgaatcg aaaaatgagc agtgttaatg
     3361 aagagatcat tgctcctttt gagagtgaga agatcaagaa aatcaagcgt caggaaaaaa
     3421 ttgaaaagat tgattcgggc ttcatgttgc catatcgtgt tcgtttttat gatatcgata
     3481 gtaatcagca tgttaataat gcgatgtatt ttaattggat cattgacgta ttaggatatg
     3541 actttttgac tacgcatgtt ccagaaaaag taattattcg atttgataaa gaagtagagt
     3601 acgggaatga gatcgaaagc cattttgaac aaatccctat cgaaaatggt gtcaaaacaa
     3661 aacatgaaat ccgaatgcaa gaacagctgt attgtgaagc aaatattata tggaaaaata
     3721 aataaaaaag aggttgtgac agaagggggc aacttcaaga aataagaaga aatttacgaa
     3781 aattgtttct caaatttttg taaattttcg acttagtttt cgaagaagct atttttgtca
     3841 cagcctctct ttttaagttt agctattaga taatgatgtt tagttttgga taaacgaaat
     3901 caattcttca cccgacaaac catagtttac agcaatcaat agttttaaac gtgctttttg
     3961 actattaata ctgttacaga aaataatccc cattttttct agttcttttc ccccgccttc
     4021 ataatcataa acaggctctg cgattccatt gaaacaacga gaaacaagta ctacagggat
     4081 tttttggaca agaagctttt ccaaagcagc taaggcttgt ggagggagat tccccgcacc
     4141 taaagcttcg attaccaaac cgtctacttt cgtatgggcg atggcttcca ataagtcccc
     4201 ttgcatccca gcatatgctt taacgatcgg gatagtccca tctacagcgg aaatgtccag
     4261 tcgctttgtt tctaaaagtt cctgcaagaa taaaattcga ttcttagtta ctaagccgat
     4321 gggacccaat gtaggtgttc ggaaagtagc aacatttgtg gtatgggtct ttgtcacgta
     4381 gcgtgcagaa tggatctcat cgttcatgac caccaaaacc cccttatcta gcgcttcttc
     4441 acaacttgcg acacgaatcg cactttcaaa attataaagg ccgtcacttc caagttcatt
     4501 acttgaacgc attgccccag tcaaaacaat tggtaatttg ttgccaatcg ttgtatcaag
     4561 aaaaaaagca gtttcttcta atgtatctgt accgtgtgtg atcaccactc cggatatacc
     4621 tgatgcataa gcggacaaga tacgttcttt taataccaac atgtgagaag gcatgatgtg
     4681 tggtgaggga agattgaaga tatcttcaac taataattct acatgcttgg ggattgtgat
     4741 ttctgcatct aataatgggt ttgccacatc aggactaaca ccgcccgtga gcatgttttc
     4801 tttcatggcg atcgttccgc cggtgtgtag tacaagtatt tttttcattt tacagacttc
     4861 cttacgaata attttgcata gccgttaaat cttattgtat actatataaa agagttttgc
     4921 tatcagtgaa gaataacgaa tagcctaact tatcataaaa aagctgacct tattaaaatg
     4981 gaaaagactc aaccttgacc tcctaataca atggagtatc tggttgagtt tcttttacaa
     5041 gaaagcagcc aaggaaatca atcacgaagg tggccggaaa tgatcagaat cggattaaca
     5101 tcgtttaaag aacatgacaa attaactggt aaaaagaata acactcttta tgaatatgca
     5161 gggtttctac ccttagtgga attagatact gcctattatg gaattccccg aaagtcctca
     5221 gttgagaatt ggataagtga agtacctgaa tcatttcgct ttgtagtcaa agtctatcaa
     5281 ggaatcagcg gtcaaggaga ctggaagcct tattatcaga ccgaagaaga gatgattgcg
     5341 cattttttag atgttatgtc gccaatggta gatgctggaa agatgttttg ctttttagtc
     5401 caattcccct catcattcaa atgtacgaaa gaaaatgtcc agtacttgcg caaacttaga
     5461 aagtggtttg agggttatcc tgtttctata gaattgcggg attattcttg gtacagcgat
     5521 gaatttcttg aacgtacaag aaactttatg tctagagccg ctttttcttt agccatcatc
     5581 gatgagccac agttgctgaa tacaacgatt ccttttgatc catttgtcac caatccgaac
     5641 tttgctcttt ttcgattcca tggtaggaat gcgcaaggat ggcaagcaaa tgataaagag
     5701 tggcgtaaaa aaagaacgtt atatcgttat tcagaaaaag aactgcaaga actagcacat
     5761 gaagtcaaag tagttgattc taagacaaaa gaggtaggtg tgatttttaa taataattct
     5821 ggtggtgatg cggctgaaaa tgcattagca ctgcaaaaaa tattaaatat caagccagat
     5881 ggactcaatc caacccaaat ggatctgttt taagggctta ttttattcaa gaaaggggcg
     5941 aagtctatga tcaaagctat tttttttgat attgacggaa cattggtcac caatcgttca
     6001 aaagcgttgg aatcaacaaa acaagcaatt gaatatgctc ggaaaaacgg cattttatgc
     6061 ggtgtagcaa cggggcgcag cccagtaaag atcaaagaaa tcattgatga attggaatta
     6121 gatatgtatg tcgtgtataa tggtcaactg gtttttactg cagatcgcac gattatcgac
     6181 cacccttttg aacaaaaagt attggaacat attgttgaat ttgcagatga gaaccatcga
     6241 caaatcgttt ttggtgcgag aaatcggtta gatggatcaa cgacgatgct attaggacaa
     6301 tcgattttta tcaagcgact agtcagcttt ttaccgcgta aattcccggt acgtctgatg
     6361 aaaaaaatcc tccaggtttt tagccctcac cgacaaaagg atcgttatga aacgttagag
     6421 atattaaaag aaccaattta tcaatgtata ttgctcagtc cggagtcaga acagaaaaag
     6481 ttagaacatc gctttcccga gtgtacgttt cagcgttcaa atacgtatac tgtagatatc
     6541 atcccaaaag gtggttcaaa attactaggc atccaagctt tcgcaaatgc tgtcgaaatt
     6601 gaaatggaag aaattatggc attcggcgat cattacaatg atatcgagat gctgaaaggt
     6661 gtaggaattg gagtggccat ggggaatgct cagatcgaag taaaacatgc tgcagacttt
     6721 gtgactcaat caaatgaaga agatgggata tacgaaggac tgaaacactt cggtctgatt
     6781 tattagtcaa tgaaagaagg aaaaaatcag tatggaagca gagacacctt ttcaaaaagt
     6841 agaaacattc cacaaaactt ttgatccaag aagaccagag atacccacgg cttttacaga
     6901 aaatgaagct catacacgtg ctgcgttcaa aatagaagaa cttgttgaat ttgttgcagc
     6961 tacttcacct aataaagaac aactgcatga agctgttatg ctgctgcacg aagcattaga
     7021 taaagcagaa gaaaaaatac ttggtaaaac acagtgggga agtgatccgc tggttgaaca
     7081 agtagatgca ctgacagata tcctttactt tacttatggt tctttttcat tactaggaat
     7141 agtgcctgat cgaatattcg aaatcgtcca tcaggcaaat atggggaaat tatttccaga
     7201 tggaaaacct cattatgacc ctgtaacaaa taaagtgttg aaacctgaaa actggcagat
     7261 ggaatatgct ccagaaccta aaatcgcggc agaaatccaa cgtcagattc aagaagcgat
     7321 tgctaagaag aaatccgctg atataggcat gtgcacaaat gaaaaagagt gatcagagtt
     7381 tcactatttt tgttatcttt atacgatgga aaaacaaaag attttcgttt aagttagtga
     7441 atgcatgaaa atagtatgct ataatctaga atagatgtct tttgcacgag agacgtatta
     7501 tttggattta tgccaagcag aacgccatct gttgcataaa agatgcatac tgaaaacgga
     7561 ggaaccataa atgggaaata atgttatcat cggaatcgtc attgcaatca ttatagtcgc
     7621 cattgtcgtc tttttggtta gttacttgat gaagaaaaaa aaccaagatc gattggatgc
     7681 cttagaaaaa cgcaaggaag aattatttga cttgccagtg attgaagaag tcgatgatgt
     7741 taaaaaaatg catctggttg ggcaaagcca aaataccttt cgtgaatgga gtcaaaaatg
     7801 gaatgacata tctacttcct cgttcgctga actagaaagt caaattttcg aagtggaaaa
     7861 cctcaatgaa agttttcggt tcttaaaagg aaaaaaagca gttgttgaag cagaagctac
     7921 aatggaccaa atggagaaac aagtgacgga aatccgtgaa ggtctaaagg aattgcggga
     7981 aacggaagaa cgcaactcac tagctgttca gcaggcactt gacgtctatg aagaactgaa
     8041 agaaaaagtc agagaagacg agcatgaata cggaccagct ctttttgaat tgcaaaaaca
     8101 gatcaaagtg atcgaaaaag aatttacgca gtttgtgact ttaaacactt ctggagaccc
     8161 tgttgaagcc cgcgaggttt tggataaagc agaaaaacat acgtatgaag tggaagctct
     8221 gatgaaaaaa attccaccgc tgtatgagga gctccatacc actttccctg aacaacttga
     8281 agaaatcagt gatacttacg agaaattgat ggaagaacaa tatgtattcc cagaagaaga
     8341 tcttgctgaa gatatcgcaa aagtaagtcg aagaattgaa aattcattag ccaatcttga
     8401 aaaaacagaa gttgaaacgg tcgaatttga aaaccgtgaa acagctgatt tgattgattc
     8461 gttatatgat atcttagaac gggaaatgga agctcagaga tatgtaaaaa ctaaccaatc
     8521 aacgattgca gagtatatca aacatacaac aaagaataac cgtcaattat tgattgagct
     8581 ggatcacaca tctcaaagct atacattgaa ccacaatgaa attggccgtg tacgaggttt
     8641 ccaaaccgaa gtggaagaaa tggaacgtca aaatgaacag atgattccac agatccgtca
     8701 acacgaaatt ccttattcag aagtgcgcac gttctataaa acagtcttca aagtcttaga
     8761 agatatcgaa aagcaacaag tagagatcga cgactcatta catgaattga gaaaaggcga
     8821 aaaagaagcg caagaaaaga tcgatacatt cgaattcaaa ttgcgcagtt tgaaacgttt
     8881 tgtagaaaaa caacgtttgc ctggcttgcc gaatgattac ctagaattct tttttgttgc
     8941 tactgaccgt attgaagaac taagtgttgt cttgaataaa attcgagtaa acatggaaga
     9001 ggtcaatcgt ttagttgctc tatgtgaaga agatctagaa ttattagata agaagacaca
     9061 tgatttagta gatgcagcag cattaacgga gcaaatgctc caatatgcaa atcgttatcg
     9121 tcatacacac gaagaagtga gagaagcaat tgataaaagc tactatttat tcaataaaga
     9181 atatcactat caagaagcat tagacgaaat cggtacagca ttagagcgag tagaacctgg
     9241 tgctttcaaa cgcatcgaag atttttattt taatcatcca gacttagtat aaagcaaaaa
     9301 agttagtaaa ccagcaagag caagacggat tattgtcttg ttcttgtctt tttttgtata
     9361 tttgaaaatt gaaacaagtg aaaatttcac ctgctcttga aagcaccttg gacaaccgtt
     9421 ataatagaaa agaatttcaa aaaagagggg tttcttatga tatattttga taacagtgca
     9481 acaaccccaa tctttccgca aagcttagat gcatatgtaa aaaccagtca gcggattatt
     9541 gggaatccat caagcttgca tgatttaggc aatcaagcta accggttgtt gcagcaagca
     9601 agaaagcaga tcgctgattt gatccatgta gaaccagaag aaatctattt tacttcaggc
     9661 ggaacagaag gagataactg ggtattaaaa ggaacgatga tcgaaaaaag agaatatggg
     9721 aatcatatga tcatttcagg agtcgaacat ccggccgtca gcgagacagc agaacaatta
     9781 aaagaattag gaatcgaagt aagtatcgcc ccagtggata aacgcggatt tgtacgtgtc
     9841 gacgaattga aagaattaat aagaaaagag acagtattag tctctgttat ggctgtgaat
     9901 aatgaagtag gatcgattca gcccatccag gaaatcagtg atcttttgga tgattttcct
     9961 aaaattcatt tccatgtaga tgctgttcag gcgattggaa aagtaccatt ctcgcaatgg
    10021 ttgacagaaa gagttgattt tgcaacattt tcagctcata aattccatgg cccacgggga
    10081 actggtttca tttattggaa aaagggtcgt cgtttagcac cattactgac aggtggaggg
    10141 caagaaagaa atcagcgaag tgggacagaa aatgttccag gtattgtggc tatggttaaa
    10201 gctttacgtt tatcattaga gataaaggaa caaaaacctg agcagacaca acgcttaaaa
    10261 gtagctctaa tcaatgcgtt gaagacgtat gagaaagtga cgattttttc agaaggaact
    10321 gactatgcac cgcatatttt atgttttgct ctaaaaggaa ttcgcggcga agttctcgtc
    10381 catgccttag aagaaaaaca aatatttgtt tctactacta gtgcctgctc ctcacgtaaa
    10441 catatggcaa gcagtacatt gcacgcaatg catgtaccaa atgatctggc tacatctgct
    10501 gttcgaatca gcttagatga aaacaattcg cttgcagaag tcgagcaatt tatgattatt
    10561 ttcaagcagc tttataagaa attttctgca atcaattaaa agaggagtgt tcttgtgaaa
    10621 tacagtgaaa ttatggtccg ttatggggaa ctttcgacaa aaggaaagaa tcgaaaaagc
    10681 tttatcatgc aactagcaca aaatgtcaga caagctttga aagactttcc agaaatcaaa
    10741 atccatgctg atcgggaccg gatgcatctt ttattgaatg gcgcagacgg gcagctagtt
    10801 attccaaaac tagcaaaaat atttgggatc caaaatttct ctccaagtat tcgtgtagaa
    10861 aaagatgtcg aagttttgaa acaagctgtt caagacatca tgaaagaaat ttatactggg
    10921 aaagaaacat ttaaaattat cgctaaaaga agcgatcacc aatttgaatt gacaagtaat
    10981 gaattgaatc aaacattagg taatgctgtt tttgatattt tcccgcatat ccaagttcaa
    11041 atgcgtcaac cagatattcc attacgtgta gagattcggc gtgatggcgc ttatctatcg
    11101 tatgaaacga tcaagggagc tggaggattg cccgtaggaa caagcggaaa aggaatgctt
    11161 atgctttctg gggggattga ttctcctgta gcagggtact ttgccatgaa gcggggagtg
    11221 gaaattgaag ctgttcattt tgctagtccg ccgtatacaa gtgaacaagc actgcaaaaa
    11281 gcaaaagatc taacagccaa actagcacct tatgtgggaa cgatccattt tatcgaagtg
    11341 ccatttaccg agattcaaga agaaatcaaa aagtcagtgc cacaaggata ttggatgaca
    11401 attactcgtc gaatgatgtt gcgtttgacc gatgaaattc gccgtattcg tcatggttta
    11461 gtcattttga atggtgaatc attaggtcaa gtggcttcac aaactttgca aagtatggtg
    11521 gctatcaatg aagtaacgaa tacgccaatc attcggcctg tagcgacaat ggataaactt
    11581 gaaatcattg aagtagcaga acagatcgat acatttaatc tttctatcca gccttttgaa
    11641 gattgctgta cgatttttgc tcctccacag ccaaaaacac gccctcaact ggaaaaggtc
    11701 catcaatatg aagagcgttt agcaatagat gaaatgatcc aacgagcact agctggcttg
    11761 aagatggaag agatacaagc agaatcgtct aatgaaaaaa ctgaagaatt ttcagaattg
    11821 ctataagagg ttatgaaaat agtgttttga ccagagtatt agagaaacaa aacgaaaaaa
    11881 tagctccatg tattttaccg tttttgaact aatgtcgaag attagctgat tgaataccat
    11941 ttatcaagaa gttatggaaa agccggttct tttttactaa ctgaaaaata aaattttatt
    12001 tagagatcaa gctacagttg ataaatgata taaaaaagag aatttcccat gaaatagcga
    12061 ggttgtgaca gaagtgggca gcttcaagaa ataagaagga atttacgaaa attgcttttc
    12121 aaatttttgt gaaatttcgg cttatttttc gaagaagtta cttctggaac accgtgtatc
    12181 agaggttg
//
LOCUS       DAZBSA010000087        12135 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000087 DAZBSA010000000
VERSION     DAZBSA010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.087"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..610
                     /locus_tag="ACHGMT_002175"
     CDS             <1..610
                     /locus_tag="ACHGMT_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN6835902.1"
                     /translation="KRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWIQLIL
                     DEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSKITVF
                     PKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAYTIQN
                     ATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            723..1001
                     /locus_tag="ACHGMT_002176"
     CDS             723..1001
                     /locus_tag="ACHGMT_002176"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6835903.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            1119..1355
                     /gene="secG"
                     /locus_tag="ACHGMT_002177"
     CDS             1119..1355
                     /gene="secG"
                     /locus_tag="ACHGMT_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFN6835904.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            1458..2213
                     /locus_tag="ACHGMT_002178"
     CDS             1458..2213
                     /locus_tag="ACHGMT_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFN6835905.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            2216..4573
                     /gene="rnr"
                     /locus_tag="ACHGMT_002179"
     CDS             2216..4573
                     /gene="rnr"
                     /locus_tag="ACHGMT_002179"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFN6835906.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            4595..5059
                     /gene="smpB"
                     /locus_tag="ACHGMT_002180"
     CDS             4595..5059
                     /gene="smpB"
                     /locus_tag="ACHGMT_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFN6835907.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            5361..6080
                     /gene="atpB"
                     /locus_tag="ACHGMT_002181"
     CDS             5361..6080
                     /gene="atpB"
                     /locus_tag="ACHGMT_002181"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFN6835908.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            6133..6348
                     /gene="atpE"
                     /locus_tag="ACHGMT_002182"
     CDS             6133..6348
                     /gene="atpE"
                     /locus_tag="ACHGMT_002182"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFN6835909.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            6439..6963
                     /gene="atpF"
                     /locus_tag="ACHGMT_002183"
     CDS             6439..6963
                     /gene="atpF"
                     /locus_tag="ACHGMT_002183"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFN6835910.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            6950..7492
                     /locus_tag="ACHGMT_002184"
     CDS             6950..7492
                     /locus_tag="ACHGMT_002184"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFN6835911.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            7515..9071
                     /gene="atpA"
                     /locus_tag="ACHGMT_002185"
     CDS             7515..9071
                     /gene="atpA"
                     /locus_tag="ACHGMT_002185"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFN6835912.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            9085..9987
                     /locus_tag="ACHGMT_002186"
     CDS             9085..9987
                     /locus_tag="ACHGMT_002186"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFN6835913.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            10010..11416
                     /gene="atpD"
                     /locus_tag="ACHGMT_002187"
     CDS             10010..11416
                     /gene="atpD"
                     /locus_tag="ACHGMT_002187"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFN6835914.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            11430..11852
                     /locus_tag="ACHGMT_002188"
     CDS             11430..11852
                     /locus_tag="ACHGMT_002188"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFN6835915.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4035 a         2155 c         2677 g         3268 t
ORIGIN      
        1 taaaagatat gcagagaaag gattcaacgt tcttgtacct gatttacgtg cgcacggaga
       61 aagtgaagga gaaatcatcg gaatgggatg gctagatcgg ctagatttaa ttgcctggat
      121 ccaactcatc ttagatgaac agccgaatgc gtccattatt ttacacggcg gatcaatggg
      181 agcttctacg atcatgatgg caagtggtga aaaacttcca tcagcggtca aaggatttat
      241 tttagacagc ggatatgttt ctgtttatgc agaattccga tatatgttaa gtaaaatcac
      301 tgtctttcct aaaaaaatgg tcatgagata tgcgaatcat tatgcccaga aatacgccgg
      361 ttactcctta aagcaagcat cagctacgag acagcttggc agcaaccatc tgcctttatt
      421 agtcatccat ggtgaaagag atcattttgt ccctacagaa gcagcgtaca cgattcaaaa
      481 tgctacggca ggggataaag cactgctgct tgttccagaa gcagaacact tggaagcatc
      541 gatgaaagat ccgaaaacct attggacagt gattttttca tttattgaac aaagaataac
      601 gatggattaa ttttctcttc ttttttgtaa gtaattcgag gataaataat tgtttataga
      661 tctggagact gctaaaataa ggtaggaata aaaaaatatc agttatttga ggagtgcggc
      721 atatggaaat caaagaagaa aaaaatcgtc ttgctttatt caatgatgag caacaagaga
      781 ttggagaaat gacatggtca gatgcgggcc cagacatcat gatcattgac catacatttg
      841 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
      901 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
      961 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     1021 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     1081 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     1141 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     1201 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     1261 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     1321 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     1381 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     1441 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     1501 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     1561 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     1621 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     1681 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     1741 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     1801 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     1861 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     1921 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     1981 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     2041 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     2101 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     2161 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     2221 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     2281 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     2341 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     2401 agtgaaactt cctttaaaac caatcttgat cgaaggaaca tttcgagcaa atgaacgagg
     2461 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     2521 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     2581 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     2641 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     2701 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     2761 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     2821 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     2881 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     2941 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     3001 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     3061 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     3121 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     3181 tgtatatttg actgatcgag tgattccgat gatcccgcaa cgtttatcga atggtatctg
     3241 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     3301 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     3361 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     3421 tcagctcgta cctatgttcc aagaaatggg tgaattgcat cgaatccttg aaacgatgcg
     3481 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     3541 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     3601 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     3661 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     3721 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     3781 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     3841 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     3901 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     3961 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     4021 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     4081 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     4141 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     4201 aggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     4261 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacaaaaag tgcgcgtaaa
     4321 agtagtcaaa gctgatccag atgaacgagc agtggatttt gaactcgttt ccgcagaaga
     4381 ggtcactgcg ttggaacgtc caaaagcacg caatggaaaa tctggaaaac gagaaggaaa
     4441 acaagcaggc aaacgctcag aaaaaaatgg caagaagaac aaacgatata gtgatagtaa
     4501 gcaaacgttc ggtaagaaaa aagggaaaaa gccattttac aaagatgtca aaaagaagaa
     4561 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaactaat
     4621 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     4681 ggtcttgcaa ggaacggaga tcaagtcgat acgaaacagc cggatcaatc tgaaagatgg
     4741 atttattcgc gtccgcaacg gggaagcttt cttgcataat gttcatatca gtccttatga
     4801 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaagaa
     4861 acaaatcgtt cgtcttgaaa acgaattgaa aaacactgga atcactgttg ttcctttaaa
     4921 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcaaaag ggaaaaaatc
     4981 gtatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     5041 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     5101 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcac aggttttttc
     5161 ttagatcagg agcaccgtgc atggggaaaa attcgcttcg tttttgactg aaataaaaaa
     5221 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     5281 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     5341 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gtttcacatt ggaccaattt
     5401 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     5461 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     5521 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     5581 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacataggtt
     5641 tagtaacaaa aatcgtctta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     5701 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     5761 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     5821 ttccgatgaa actgattgaa gaatttacaa atttattaac acttgcttta cgtctttatg
     5881 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     5941 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     6001 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     6061 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     6121 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     6181 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     6241 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     6301 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     6361 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     6421 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     6481 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     6541 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     6601 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     6661 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     6721 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     6781 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     6841 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     6901 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     6961 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     7021 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     7081 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     7141 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     7201 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     7261 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     7321 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     7381 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     7441 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     7501 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     7561 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     7621 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     7681 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     7741 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
     7801 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
     7861 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
     7921 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
     7981 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
     8041 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
     8101 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
     8161 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
     8221 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
     8281 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
     8341 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
     8401 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
     8461 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
     8521 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
     8581 cggttgatgc cggactatct gtatctcgtg tagggggttc tgcgcaaatc aaagcaatga
     8641 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
     8701 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
     8761 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
     8821 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
     8881 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
     8941 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
     9001 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
     9061 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
     9121 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
     9181 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
     9241 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
     9301 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
     9361 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
     9421 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
     9481 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
     9541 tctgatcagc caagctttga tgaagtccgc aaaatcgtaa atatggcaac aactatgttc
     9601 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
     9661 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
     9721 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
     9781 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
     9841 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
     9901 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
     9961 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    10021 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    10081 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    10141 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    10201 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    10261 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    10321 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    10381 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    10441 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    10501 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    10561 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    10621 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    10681 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    10741 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    10801 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    10861 attacaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    10921 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    10981 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    11041 ggcttcttct tcaagtgctt tagcacctga aatcgtgggg gaagaacatt ataaagtagc
    11101 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    11161 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    11221 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    11281 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    11341 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    11401 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    11461 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    11521 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    11581 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    11641 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    11701 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    11761 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    11821 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    11881 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    11941 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    12001 tggaacaccg tatatcagag gttgtaaaat cagcgttttg tcctgagtat tagagaaaca
    12061 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    12121 tgaacaccgt ttatt
//
LOCUS       DAZBSA010000088        11765 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000088 DAZBSA010000000
VERSION     DAZBSA010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.088"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(176..790)
                     /locus_tag="ACHGMT_002189"
     CDS             complement(176..790)
                     /locus_tag="ACHGMT_002189"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFN6835916.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(937..1620)
                     /locus_tag="ACHGMT_002190"
     CDS             complement(937..1620)
                     /locus_tag="ACHGMT_002190"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFN6835917.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1729..2187)
                     /locus_tag="ACHGMT_002191"
     CDS             complement(1729..2187)
                     /locus_tag="ACHGMT_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFN6835918.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2271..4034)
                     /locus_tag="ACHGMT_002192"
     CDS             complement(2271..4034)
                     /locus_tag="ACHGMT_002192"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFN6835919.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4255..5715)
                     /locus_tag="ACHGMT_002193"
     CDS             complement(4255..5715)
                     /locus_tag="ACHGMT_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835920.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5923..6849)
                     /gene="glsA"
                     /locus_tag="ACHGMT_002194"
     CDS             complement(5923..6849)
                     /gene="glsA"
                     /locus_tag="ACHGMT_002194"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFN6835921.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6872..8299)
                     /locus_tag="ACHGMT_002195"
     CDS             complement(6872..8299)
                     /locus_tag="ACHGMT_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN6835922.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAYLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8330..9544)
                     /locus_tag="ACHGMT_002196"
     CDS             complement(8330..9544)
                     /locus_tag="ACHGMT_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFN6835923.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(9630..9857)
                     /locus_tag="ACHGMT_002197"
     CDS             complement(9630..9857)
                     /locus_tag="ACHGMT_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835924.1"
                     /translation="MKNKFENRSDTRCGSFLYILYEVFFIPYEKISLKIKKDENKRFS
                     IKRRPFIFCLYLCEKRKRAQHIKLLLIVCLL"
     gene            complement(9885..10127)
                     /locus_tag="ACHGMT_002198"
     CDS             complement(9885..10127)
                     /locus_tag="ACHGMT_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFN6835925.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(10159..10716)
                     /locus_tag="ACHGMT_002199"
     CDS             complement(10159..10716)
                     /locus_tag="ACHGMT_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFN6835926.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(10729..10917)
                     /locus_tag="ACHGMT_002200"
     CDS             complement(10729..10917)
                     /locus_tag="ACHGMT_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFN6835927.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(10930..11496)
                     /gene="amaP"
                     /locus_tag="ACHGMT_002201"
     CDS             complement(10930..11496)
                     /gene="amaP"
                     /locus_tag="ACHGMT_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFN6835928.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         3450 a         2440 c         1926 g         3949 t
ORIGIN      
        1 tttctatttc cttaccatct aaagtaacag tagtaacgac aaaatggtct tgcaatggcg
       61 gaacttctaa gtactgccct tgcagaaaat cattcactgt ttcatattct tttttcattt
      121 cttttgaatc tgtcgatggt ttatagctta tttttaacat aactagtgct cctttttatt
      181 cgggacgtgc aatcgtgaaa atttctccta ttgctgcata atcatttcct gccaaacggc
      241 ttacagcatt caattttctt ggatcgatcc ttcctgtctt atcatcatag actgtttcat
      301 ttaaatgata tttctgtact tctagcaaaa ataaatcagc tgttacttta tttttattcc
      361 gtatctcgac atgttcatat aaggatgttt caaaacgaac ttttgcttct gtcaatgctg
      421 gtactgaaat gattactgaa tcagttaaag tcaagtcagt caggtacagt tcgctttcct
      481 cattaggcag attggctgct gttttattta cttcttctac gttgtcctca tcaaggatat
      541 ggataacagc ttctcttgtc tcaagaatat tctttgctgt gtcttttact gcatcttgtt
      601 ttctctggat cgccaaggat aagatgggtc gattcgaact gacgatattg aagtaactga
      661 agggtgcgat attcactata tcttttgctg atttagtcgt tactactgct actggtctag
      721 ggatgactga accaatcagc aacttgtaat tttcacgttc tgtcaattct gaaggactcc
      781 gttcaaacat ggtttcgcct tctttctata aaattcgtta aaaagcagat cttgaagaca
      841 aaatgaaaag taatgctttt ttcatcttct tagaatctgc tttaaactat ctatcaattt
      901 ttcttttttg tcaagaatct gtgccgtgcg tgttttttag ttatttgctt tacttgcggc
      961 ttcgacatat cttttagtga tcaattgcgg acgggtgata atagatccaa ctacgactga
     1021 aaaagcacct aattcatatg ctttctctac ttgttcagga gagtcagtat gtccttccat
     1081 aattactggg atatcgactt gttctatgat ttgattcaat aaatcaaaat caggtgtctg
     1141 cgtctttcgt gaatattctg tatagcctgc taaagtcgta gacaaaagat caaaaccatc
     1201 ttcttgcgct tgaagcgctt cctcaaaagt cgaaatatct gccatagcta accgaccgtg
     1261 ttcgtgaatc atttggacaa gttcagcagt tgttttttct gtcggacgtt tacgagaagt
     1321 tgcatccagt gcaatgattt ctgcttgcgt ctccaaaagt tcttccactt ctttttctgt
     1381 cgcagtaata aacacttctg acccatcgta atctcttttg ataatcccta ttaccggtaa
     1441 atcaacgatt cgcttgattt cattgatatc ttctgtcgta tttgctcgaa taccaactgc
     1501 tccgccttct tttgcagcca aagccattct ccccataata aaagaactgt gtagcggctc
     1561 gttttccaaa gcttgacaag aaacgacgac tccgcctttg atttgttcca gtgtatccac
     1621 aatatctcct ccagtaatgt tatataaaaa tacagcttca tataaaaatg taaccctttt
     1681 ctattcagga gacaataatg agctatttct ttcagcagac ctttcttact aaaagaaaat
     1741 tacttcaaat tcttcgtata cgaccagctt ctcttctgta aattctatgt tcaacgcttc
     1801 tttataagca tgtttaaaaa actcagcatg gactttccgc caataagcta atgtccgatc
     1861 cccttctcct tctttccaag catgctcttt cgtgacttca ctaaaaggca agatggtcac
     1921 atttatattt ttgatgatac aaactggttc attttttcca ttcaggacca cgtctaattg
     1981 accaactttt ggtaaaggct cattctcttg tttatacaga tcgtaaccac ttgatgtacc
     2041 agtctttatc ttttttacaa ctagttcgct caactcgtct gccatttttt ctgtatcccc
     2101 aaatgaaaag aaacctaatt tttctgattg ataactagga tatttttctt ggaacttttt
     2161 gacatattct tctacttgct gatacataac atccacccct ttgcttttta taatgaaaaa
     2221 accatccaga tactagtata aacaattatc tggatggtga gcataatgaa ttattgaata
     2281 ttcaagacac gcagactatc ttcaacgatt tgttttggtg ttaaatgata acgttcataa
     2341 agctgcccta caggaacgct atccgtgaat tctttctctg caccaaaatt taatacgttc
     2401 attgaagaat caccataata acgactgata ttttcgccaa atcctcctga caaactacca
     2461 tcttctaatg tgatgactaa ttcgtgtgtt tcttttaatt tatctaatgt ttctacgtca
     2521 ttttctgtga tcgttcctgg attgatcaac gttgcttcga ttcctttttt tctaaaagct
     2581 tttactgttt cttttcctaa ttcaagaaaa ccgcctaaag ctaaaattgc tacttttcct
     2641 ccattattta ctgtttgata ggctggaata ctataatcat ttgcgatagc tgtaccatgt
     2701 tctacaccat gtgtaggaat tcggattgct acaggctgcg ctgtttgttc gaacgcccaa
     2761 tctagcatag agatcatatc ctcttttgtt gctggagcaa gatatttgat gttcgggata
     2821 cttgataagt aaggaatgtc aaatgttcct tggtgtgttc ggtcactagc tgagatcgtt
     2881 ccgccaccga ctaaaataat cactggattc tcgttgatcg ctaaatcatg tgataattga
     2941 tcatatgccc gttgcaagaa agtacttgat tggaagacga ctggacgtac tccctgagaa
     3001 gccaaagcag aagcgaaagt gatagagtgc tgttcagcga tccccacgtc aaaataatga
     3061 tctggatgtt tttcttggaa tgttttcagt ccaaaggcgc ctggtatcgc tgcattgact
     3121 gcggcaatag gagctgtact tgtttgcatt ttcttatcca aataatccat gatgatcata
     3181 ttaaatgttt ctccagaagt tacttgcttt gcctccccgg tggacagatc aaatggtaca
     3241 tgccaatgat atttcatttt attatcgatt gccggctgat aaccgcgacc tttttccgta
     3301 tggacgtgaa gtacaatcgg atgatcaata tcttttactt cttcaaataa acggatcacc
     3361 gtttccagat cgtttccatt atccacgtaa cgataatcaa atcccattgc tttaaatagg
     3421 ttattgctgc ttttaccgtt ggtttgacga agctcagcta agtttttata taaactgcca
     3481 tgattctcat caatagacat ctcattatcg tttactaata cgattagatt ggattgtaaa
     3541 acaccaccat tgttcaatcc ttcaaaagct aagccaccac ttagggagcc gtcaccgata
     3601 aatgctacca catttcctga ttctttcttc agatctctcg ctttagctaa acctgttgct
     3661 aagctgatag atgtagatgt gtgccccact gtaaaatagt catgtttact ttcatgttgg
     3721 ttggaatatc cgctcacttc acggtaatgt cctttttcaa aaccattttt tcgaccagtt
     3781 aaaagcttat gaccataaga ctgatgggaa acatcccaaa cgattttatc ctttggtgaa
     3841 tcaaatacat aatgaaacgc aatcgttaat tctactacac ctagattcgg accaacgtgc
     3901 ccacctacat cgctgtcctt ttgcaagatt ttttggcgca tctcttctgc caactgtttc
     3961 atttcctcaa cagataattt tttgacatct gcggggctat tgattgcatc caatacatgt
     4021 aatactgtac tcataataca ttctctcctc cgtactatct tcttttaatg aaaatagatt
     4081 tgttacactt tatctattct actacttaga gttgactcta agtcaatagc attttaaaaa
     4141 aatttcagag aaagcttgtt atataagtag atcttcgcac gaatcaggac tcaaataaaa
     4201 accttctaca gacaattaaa aagctttgta gaaggtctcc taattaaggt ctcatcaaga
     4261 aatatgtttt tttaataggt gaagttcctg agcaatatga tacaaatgga tgtttgcacc
     4321 ttttaatgag attggataga tctttgtctt ttcagtttct aatccatttt gatagctagc
     4381 agcatcgcac aaaaccactc tttttttcgc acatagacat tcatcaggca attcttctaa
     4441 tgaacgaaat aaaataggat ttatttcagc gatgccttct tccgcataca aatgaaattg
     4501 ggttcgataa aaaaattgtt tgactgaact ttctgaaaca acaaaaattt ctatttcctg
     4561 tctccccgct tgttcactgt tgatcaaaga aagacttttg acgaattttc gaatgaaatt
     4621 ttcattccat cttagttgga tttgattttt ctttttccac tcttccaata tttctacaat
     4681 ttttccgtac agaagttgtt ctttttctga tagaagatag atcctttccg gtaaaaatac
     4741 ttgtccatct gcccacaacg tacggacaaa ttgtaaaaag gattgcttga aaataaaatt
     4801 ttctgcatcg tcgatttcag cgatactaat aatctgtaaa atcaattcat tcagtgatag
     4861 gtcattgaga acaaaatttt tataaacaac ttcgaaatct tttcttaaca gctccatgtt
     4921 ctcattggta taatttctac tgttgaacaa agaaaaaata taatagacat cttcttcttg
     4981 taagtctaga attccttttt gctcttccaa tccttgacag actaattgaa ataacggctt
     5041 cgtttctgcg cttttcttat cttcttcttt cagtccaagg ggaaatgcct ttcttccgat
     5101 tgcgatctct attcctcgta tgataaagga cttttcatct tccgaatatc ttcttttaaa
     5161 aaaatgagtc tctacataat caatcaactc gttacaagct tcttctacat acggattttt
     5221 attttcatag ccttcccaat tgacataacg tactaaagct agcaagatgt tccgcgagtc
     5281 aaattctggg ataatgattt ctttattttt caaataatca ttttctttaa aaaaatccaa
     5341 tacgattttt ttgattgtat atactttgct caaagaaata aattcaagat cagccagttc
     5401 tgtactcgtg aatataccat taaaataata acttaaaaac tttaaaaaat aagattgacc
     5461 gtacagaaac tgtgtgagct cagaaagagc aataccatgt ttgaatacga gtttatactt
     5521 atgcttctct cttacttgtt cttttataca atattctaga cactcggcta gactttctag
     5581 atcgtgaagg atcgttggtg tagtcaccct tagagaaata gctgtttgtt cgatgtctat
     5641 ttcttggaat tcaaataatt gttcacacag atacactttt ctaaatatat ttcgctcgat
     5701 gtaattttcc agcattcctg atacacgcct ccagtttttc ttatgatttt atttaatcat
     5761 atctgtaaat cgtatttttg tttccttttt atttagaaaa gccctttcaa aaaccaataa
     5821 aaaaagaaac tgggacaaaa gtcaaacaag ctcacaattt cagcctcatt gtaggtagct
     5881 ttgagccgtt tggactttta ccccagcctc tttcaagaat ggttattcaa agatgtcagc
     5941 gtctagttct ttgactacat ctttcaacaa ttggatacca gctgcgctgt taccaaatgg
     6001 atctagtgcc gggctgaaga cacctatgcc gtaacgattt ggcacagctg ccatcaaccc
     6061 gccgcctaca ccgctttttg caggtacacc aacatggacg gaaaactctc ctgattcatc
     6121 atacaaacca gcagttgtca taattgattt gacgattgta gcactttctt cagtaatgat
     6181 ttgtttgcca ttccaaggag caattccttt atttgccagc acagcagcta attttgctat
     6241 gcctgtagcg gtgacattga gggagcattg tctgaaataa gcatcgagta tctccggtac
     6301 gtctccttcg atcatttgat tacctttcat atagtaagca agagagcgat tgatatcacc
     6361 tgttcttgat tcagaatgat agatttcttc attcaatgtg atattaggat catcacagat
     6421 ctctttcata aatgacaaga ttcgctcaaa tttttcatct gcatctttac cggcaatcaa
     6481 cgaggttgtt gcaatcgctc ctgcattaac aaaaggattc attggatgtt tgcgatgatt
     6541 gatttccata ttcatgatag aattgaaggc aaaaccggtt ggttccgtgt tgattgtttg
     6601 aaagactttt tctattccgt tgtctttgat tgctaataac aatacgggaa cttttgacat
     6661 gctttcgatg gcaaagcgca catcggcgtc tcctccctca atcagttgtt ttgtatccaa
     6721 atcataaatc gccataccta attgtttggg atccacttca gctaatgctg gaatgtaatc
     6781 cgccacttgt cctgtttgat aatagtggaa attttttcca atcaattgtt ctaattcttt
     6841 tacttccatt tgaataacca gctttcattt tttatttttc tgcatgctgg aatttatgac
     6901 ggcatgcgta gatcgtaaat ggaataacta agacgataag ataagaaaca atcagaatcg
     6961 tttcataaat cccatgctgt ccagctgcga tactagcagg tggaacaaat gagatacaaa
     7021 aagcaaaaag tgaagtcaaa agacctaaac aagcaacgat tacttttaca acttttccgc
     7081 ctggaacctg ataggctctt tcaagactgt catctttaaa aaccagcttc aaatagcccg
     7141 cgaaaaacat aaagtacaac atcaggtaga ttacaacagt caatgacatc gctgcaagaa
     7201 atgaaagatt attccccccg ccgcttaatg taaagacggc tgcccagatt gttgcaacga
     7261 tcccatttgc gatcatcaaa taagcaggaa caccgtgtgt gttttctttt gcaaaatatt
     7321 caggaagtaa tccttcttct gctgctacgt gaagtgcttt tgaaggagag gttacccaag
     7381 aagcaatttc tccgatgaca cctaaacaaa caagaatcgc aagaattttc gcaatccatg
     7441 caagatttgg atcaatgtgt cttaatagga attctaacga ttgaatcacc cctgtattaa
     7501 gagacaaacc atgttgaggt actgtagtag cgacggtcaa tccgccaaaa gtatctaaaa
     7561 tgattgccag aataaccaat acaaacattg ctaatggata atccttttta gggttttcca
     7621 tttcatttgc atgactggca gatgtttcta cgcccatata tgctaaaata aaggaaacaa
     7681 aaacaaccag cgtaggcaat ttcgaaaaat caggaatcaa tgttttcata gacagtatcg
     7741 tttgtagtgg agcgcctgaa gcgacataat ggatccctaa gccaaacaag actaatgctg
     7801 gcaacaagat accagctaca aaaccgatcc gagcaatcaa tgaagtgtat ttggttcctt
     7861 ttaactgtga gagtaaaata gcccagaaaa taatcaatag gattgccaat ttcagatatt
     7921 tattgttttg tatagcacca atacccgttg cataggataa tgcaccagtt aagaaataca
     7981 gcatggtcaa gtagccaact gttatctcaa accattggaa aaagattgcg gcaaaaccga
     8041 atcgttttcc caatgtgcga ctgacccaag tatagacacc gcctttttcc catcctttca
     8101 ccgttgccat ctctgctgcg cacaatgcca cagggataaa ccatagcaaa ccgcctaata
     8161 acaagaaaaa tactaagctg aagcctgagg tcgcgaatgt gggatattca tataatgaaa
     8221 cgaccattga gatggtcata gccaaaaagc caaagaacgt taattttttt gcaggaattt
     8281 tcgttttgct ttgttgcatg aatcttcctc ttttctattt gaatttgttt caattgagtt
     8341 tgttgccaaa gaagaaaaag tccaatcctt cttcttgttc ttcttttgta gtagtgatcg
     8401 ttgtaaagtg catcgccgat tttaaaatca ctgttgtcag cacataggca tacacggcag
     8461 taatgatgac accaatcact tgaatcccta agaagtacca gctgcttgga ttcgttagta
     8521 aatggctgtt agatgcaaag atccctatca gaatcgtacc tgtgaatccg cccatgccat
     8581 gaacacccca cacatcaagt gcatcatccc attgtttctt tttacgaaaa tcaacagcag
     8641 catgacagac gatacccgca atgatcccaa tcagcatggc actttttgct tctacataac
     8701 cggcacaagg cgtaatggct gccaatccag caacggatcc tgttagtacg tcgacaaaat
     8761 caaagccatt tccttttact tttgcaatga tcagccaagt aatcatcgca aatgcaagag
     8821 cgatgaatgt tgaaccaaat gcattgactg cttgttcatc tgctcgaagt gcaccaccgg
     8881 aattaaaacc gaaccagcca aaccaaagca gtcctgtacc gatagcagct gccataagac
     8941 tgctatgctg acttctttct cctaaaggaa tctttctttg accaagtgtg aggatacatg
     9001 ctaaagagcc gaatccagct gtcgtgtgga tgactgctcc accagcgaaa tctctaaatc
     9061 ccatctgatc caaaaatccg ccaccccata tccagtggca aacagggaaa taaatcagca
     9121 gattccaaaa aataacaagt ataatatagc cttttaaatt caatctccct gcaaaagctc
     9181 ctgtcatcaa aggaacggta atcacgataa acatcagttg gtaaagaaag aacattaaaa
     9241 atggaatcgt tgcaccatga aacatgttgg ggaagcttgt aacatgtctc atcaaaaaga
     9301 aatctgccgg gtttccaata acaccgccga tatcccgtcc aaaggccaat ccgaatccac
     9361 caaataccca cataatcgtt gttatcccaa cggagagaaa agattgagac atgatcgtta
     9421 gtatattatt cttccgtgct aatccgccgt aaaaaaaagc tagaccagga gtcatcaggc
     9481 aaaccattgc tgtacataaa atcatgaatg ctgtatctcc tgtattgata ggtaatgtat
     9541 tcattttttt cgcctccttt ctacgattac gagtataaaa gaaggtgtag ttgttttttt
     9601 cttctgtttt tttgaattaa tacttgcatt tataaaagac agacaattaa taaaagcttg
     9661 atgtgttgtg ctctttttcg tttttcacac aaataaagac aaaaaataaa cggtcttctt
     9721 tttatagaaa accgtttatt ttcgtctttt tttattttta aagatatttt ttcataagga
     9781 ataaagaata cttcatataa aatatataaa aaagaaccgc agcgagtgtc gctacggttc
     9841 tcaaatttgt tcttcatttc aacataagtt tttcttcaat ttgcttataa tttacgtgca
     9901 aagaatgaaa cgactaatac taaaatgatt gcaccgatca atgatggaat gatagccatt
     9961 cctgcaattg ttggtcccca agttcctagt aaagcttgtc caattgctga acctactaaa
    10021 ccagcaatga tgtttgcgat gatccccatt gatgatcctt tattcgtgat tgctcctgca
    10081 attgctccaa tgataccgcc gacgattaat gcccaaataa atcccatttt aaattcctcc
    10141 taactgactt cttacttctt attgtacacg tggttcgtta ttttctttga ctttttgtgc
    10201 acctttgcct acagcagatt ttgctttatc tgtttggcgt gaagcaaatt gtcctgtgga
    10261 ttttgctgct tctgttactt tgtcttgtac tgtttcttga tctttttggt attcttcttg
    10321 tgttttgatg tctgcaacat tcacattcac ttcgataaca tctaaatgtg tcatattgtt
    10381 tacttcagta ctgatcaatt ctttgatttg gttgtaaata tcttcgatgt ctttaccgta
    10441 ttctacgatc acatctagat ccacagctac ttgtttctta cctacttctg tattgatacc
    10501 tgctgttacg ttatctgtgt tgactaattt ttcagccata tttgagaaaa atccgccatc
    10561 taatgtcaat agaccatcta catctttcaa tgcataaccg atgatttttt ggatgacttt
    10621 gtcttcataa gtcaattcac cattcatcat gcggttttct cctttttcat ttgccaaaac
    10681 tgatccattt gttgtattca tgtttttgtt ttccatcttt gtttcctcct atgatttaaa
    10741 aaattgatca actattcctg actgtttcaa ataaaaaccg attcctgcgc caattgctgt
    10801 catgattatg atcaacaatg tcttgaaaaa tccaatcgtt aaccataaaa cagctagcag
    10861 tagtccaaga cccccaaaga taatagggag cttgtatgct gaaaatagtt ctttcattat
    10921 ttggccctcc tattccactc gagagtgctt gtagtctctt gtatcttctt gttcttcata
    10981 accgctgtat gtgactgcca ctttgaccgt ttcttttgtt ccgagtactt tagttacttc
    11041 ttcttcgata tcttgcatca atacacctgt tttccctact aatgaagaag ttcgtttcaa
    11101 gtctccgttg acatggatat ggattcgatt tttcgttgaa cgtatccgta ctttaggtga
    11161 atgaatgaac tcttcttcgt gtaaatggga acgtaccaac ccttcgatcg cttttttgtc
    11221 gattgttagt ttgccatctt cccgtttcat aacgaaagtt cctcttgact ttggatagaa
    11281 aagaacgact agcaagatga taatagcaag aacggcaaat actacagctg cccagaaaat
    11341 atattgttgc atgtagtaat tcgttaaagt aaagaagcga aatcgttcca attgttcagg
    11401 cataatatac ggcgcattga tcaacgcaat cgctagaaat actgaaagta gcagtaaact
    11461 catgataaca ccgattattt ttaacccctt attcattttc cacctcctat aaacttattt
    11521 ttaccctgta aataactcta ccgacaatca cagtatagat agcgaacagt tagcatagca
    11581 tcagtaaata aagcgctacc aaacattgct ggatcataac ggcgttttat tgccagtctg
    11641 atactgaaat aacaattgct tacttattct ctatttactt ttgattgctg gctatactta
    11701 aaggatatta ttcaagatta aaaattgcaa agattatgct cagttattat tacaagattc
    11761 tattt
//
LOCUS       DAZBSA010000089        11688 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000089 DAZBSA010000000
VERSION     DAZBSA010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.089"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..629)
                     /locus_tag="ACHGMT_002202"
     CDS             complement(105..629)
                     /locus_tag="ACHGMT_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFN6835929.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACHGMT_002203"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACHGMT_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFN6835930.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACHGMT_002204"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACHGMT_002204"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFN6835931.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="ACHGMT_002205"
     CDS             complement(2351..3031)
                     /locus_tag="ACHGMT_002205"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFN6835932.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="ACHGMT_002206"
     CDS             3157..4026
                     /locus_tag="ACHGMT_002206"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN6835933.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="ACHGMT_002207"
     CDS             4052..4567
                     /locus_tag="ACHGMT_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFN6835934.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="ACHGMT_002208"
     CDS             complement(4614..5177)
                     /locus_tag="ACHGMT_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6835935.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="ACHGMT_002209"
     CDS             complement(5232..5411)
                     /locus_tag="ACHGMT_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835936.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="ACHGMT_002210"
     CDS             complement(5488..5997)
                     /locus_tag="ACHGMT_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835937.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="ACHGMT_002211"
     CDS             complement(6093..6473)
                     /locus_tag="ACHGMT_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN6835938.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /gene="pnuC"
                     /locus_tag="ACHGMT_002212"
     CDS             6803..7552
                     /gene="pnuC"
                     /locus_tag="ACHGMT_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFN6835939.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7736..9013)
                     /locus_tag="ACHGMT_002213"
     CDS             complement(7736..9013)
                     /locus_tag="ACHGMT_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835940.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="ACHGMT_002214"
     CDS             complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="ACHGMT_002214"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFN6835941.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9935..10879)
                     /locus_tag="ACHGMT_002215"
     CDS             complement(9935..10879)
                     /locus_tag="ACHGMT_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFN6835942.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10881..11567)
                     /locus_tag="ACHGMT_002216"
     CDS             complement(10881..11567)
                     /locus_tag="ACHGMT_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFN6835943.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         3336 a         2472 c         2049 g         3831 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaataacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
     7741 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
     7801 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
     7861 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
     7921 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
     7981 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
     8041 aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
     8101 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct
     8161 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
     8221 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
     8281 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
     8341 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
     8401 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
     8461 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
     8521 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
     8581 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
     8641 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
     8701 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
     8761 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
     8821 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
     8881 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
     8941 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
     9001 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
     9061 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
     9121 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
     9181 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca
     9241 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
     9301 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
     9361 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
     9421 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
     9481 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
     9541 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
     9601 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
     9661 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
     9721 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
     9781 atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca
     9841 gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt
     9901 gcgacaatta ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa
     9961 tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt
    10021 ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca
    10081 gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat
    10141 gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat
    10201 tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca
    10261 catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc
    10321 ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg
    10381 tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga
    10441 acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat
    10501 tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt
    10561 tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc
    10621 ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca
    10681 cgattgccgg aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg
    10741 ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca
    10801 atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag
    10861 gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc
    10921 ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact
    10981 tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg
    11041 actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt
    11101 tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct
    11161 tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact
    11221 ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca
    11281 atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga
    11341 ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga
    11401 ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc
    11461 aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac
    11521 ccgagaataa caatcaagac gcctaaaagg ctaagataat tttccatata ttttcctcca
    11581 taaaatgaca atattttttt aattataaag caaaacacat aaaaaatata gttgttttct
    11641 cacgaaatag acactatttt taaatatttt tcgttatttt tttatcac
//
LOCUS       DAZBSA010000090        11348 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000090 DAZBSA010000000
VERSION     DAZBSA010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.090"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(121..1005)
                     /locus_tag="ACHGMT_002217"
     CDS             complement(121..1005)
                     /locus_tag="ACHGMT_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFN6835944.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(1092..1991)
                     /locus_tag="ACHGMT_002218"
     CDS             complement(1092..1991)
                     /locus_tag="ACHGMT_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFN6835945.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            complement(2008..2514)
                     /locus_tag="ACHGMT_002219"
     CDS             complement(2008..2514)
                     /locus_tag="ACHGMT_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFN6835946.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            complement(2703..3323)
                     /gene="rpoE"
                     /locus_tag="ACHGMT_002220"
     CDS             complement(2703..3323)
                     /gene="rpoE"
                     /locus_tag="ACHGMT_002220"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFN6835947.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(3399..3836)
                     /locus_tag="ACHGMT_002221"
     CDS             complement(3399..3836)
                     /locus_tag="ACHGMT_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFN6835948.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            3999..4829
                     /locus_tag="ACHGMT_002222"
     CDS             3999..4829
                     /locus_tag="ACHGMT_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFN6835949.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            4813..6180
                     /locus_tag="ACHGMT_002223"
     CDS             4813..6180
                     /locus_tag="ACHGMT_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFN6835950.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            6199..7008
                     /gene="yidA"
                     /locus_tag="ACHGMT_002224"
     CDS             6199..7008
                     /gene="yidA"
                     /locus_tag="ACHGMT_002224"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFN6835951.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(7051..7494)
                     /locus_tag="ACHGMT_002225"
     CDS             complement(7051..7494)
                     /locus_tag="ACHGMT_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFN6835952.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            7628..7999
                     /locus_tag="ACHGMT_002226"
     CDS             7628..7999
                     /locus_tag="ACHGMT_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN6835953.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            complement(8168..8950)
                     /locus_tag="ACHGMT_002227"
     CDS             complement(8168..8950)
                     /locus_tag="ACHGMT_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFN6835954.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(8947..9918)
                     /locus_tag="ACHGMT_002228"
     CDS             complement(8947..9918)
                     /locus_tag="ACHGMT_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFN6835955.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            10010..>11348
                     /locus_tag="ACHGMT_002229"
     CDS             10010..>11348
                     /locus_tag="ACHGMT_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFN6835956.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLE"
BASE COUNT         3357 a         2300 c         1979 g         3712 t
ORIGIN      
        1 aaaattggtg gacagttttt tgttgtacct gtatagatat tgttaagcct ttgcaagtaa
       61 ggtagttatg tgttcaacta cttattaaaa tgtgttattt attaaagata cttgtctcac
      121 ttactgaact ggccgatgat cgtttctgcc ataatagttc tcgacagccg ttggatgccc
      181 taataaatcc ccaactgtcg tgatgcgata cccttgctct ttcagataat cgatcacttg
      241 aggaacggca cgaatcgttt caggatagat atcatggaat aaaataattg ccccatcgta
      301 tactgttgct tggatcttct gcaaaatcaa atcaggattt cttgtcttcc aatcttctga
      361 atcaacagac cattcgatga tcggtcgctg gatgatcgtt gctcctacat tcgtgacaga
      421 accatagggc ggacgaacaa aagtgggggt tttgccagtg acttttttga tctcagtgtc
      481 tgctttcgtc acttcctccg tcatcgactg ggcagaaaga gtggccagat tctgatgatc
      541 gtatgtgtga ttgccaatct cgtgccctgc ttcagctgtc tgcttcacga tttcaggatg
      601 ctgcgccgta ttttctccta agacgaaaaa agtggcatgc acgttttttt cttttaagat
      661 tcgcagaagc tgcggtgttg tggtcgctcc tggtccatca tcaaagctga ttgcgacgct
      721 tttgacagcc ggatctcggc tcgtttttgt actgtcttgt gttgattgtg atgactgact
      781 ttgtaccttt tccgctgatt cttgaatggc ttctttgacg ttagctgttt cagttgattg
      841 gcttttaatg gttgattgtt ctgattgttt gaaagattgt gacgaatgct ccgaagagtg
      901 tgtttgtcct tttacttcgg tgatcaagga ggccaccgaa gtaatttcga aaaacaaaag
      961 cacggataaa gcaataatac tgataactaa ataatttctt ttcactatat ctcttctttc
     1021 tctttttctc ttcttattat ttacttgtac ttacttgaaa attgaaactg ctagtcgcaa
     1081 ctacttctcg tctaatgatc caaataatct tccgatgcca aaatcgtttt tgtcacggac
     1141 ttaggaatga caaattctgg tgtacccata taaccaggag caatttcgta ttgagaaaaa
     1201 gtcaaaacta attgatgctc tttgttcaaa taaaaacttc gtgtaggcgt aacaagtgtt
     1261 ggtttttcta tcgtcccttc ttgaatatct tgctcagtcc aatagtactt agatggatct
     1321 tccttgactt gttcggccat ctgccgtttg acttcttttg tcaaaacaga tagataggtg
     1381 tcatctttga ataaaagtgg aagtgagagc tggacactgt ttttcttgtc gattgtgtcg
     1441 tatctttttt caacatggct atctgctgct gtttgggtga tctgcctttc aatcacgaga
     1501 aaccggttat cgtctacgat tttctgataa tctcccaaaa cttggtaatg atccgtttca
     1561 aaatttccat attctttttc agctttttca aaatctgctt gtccttcgcg aaagtatttt
     1621 tgatttaatc catgaattgt tttattttct ttctcatttt gtgtattgat cttcggtacc
     1681 tgaatatcga ttttttcaga tcgatcagta aagcgttcgc ctaagatcaa ttcaatgact
     1741 gatcctaaga tgggtaaatc ttctgcaaag cttcgaactt gactattaaa aagaaggcta
     1801 cttgttgtaa tcattacgat agccaaagat actccgaacc cttggagacg tctctttctc
     1861 cgctttgctt ttataaatga ttgctcttcg ataataaaac gctggcggat ctcctcttta
     1921 agccctttgg gaattgcttg ttctttataa gttttcttta gtttttttaa attttctttt
     1981 tgggaataca tggcactctt ctctccttca ttctaggatt tgacgtaaag cattcaatga
     2041 gcggtacatt ttcgttttaa ctgtgttttc attcatatcc aagatagtcg caatttcttt
     2101 caaacgaaat ccttcaaaaa agtagaggat cagaatctct ttttccggac tgtctatttt
     2161 atctaaaatc atatggagct ctgcaacgga agtttctaaa ggtaactgtt gggggatatc
     2221 ttgaatttca aataagtcca caggcgtcct tttttgtctg cgccaactat ctaatgccgt
     2281 gcggatcaat atccgataaa accatgcact aaaatgctcg atctcttttt ctttttcaaa
     2341 atagttcagg gctttatgaa aactatcttg catcacatct aaggcctctt ctttatcatg
     2401 tacgtaattc atcgtgatac ggaacatttt ttgataatca gtttcgatga gtccattcag
     2461 caggtgacgg tatttttcga tctgccgttt tttcttatat ccagtaaaaa tcattctcca
     2521 ctcctttctg cctcacttaa tatgacgtac aaggtgcgct aaaagtttca atttttttat
     2581 tttttatgta tttacttggg atttttatat acaaaaaaga aggtgtgaca aaaatcgtgt
     2641 cacacctcct tattctgata ggctgatcgt ctctgtcaca acctctcgta agaaaataat
     2701 gtttattctt tttcagagtc agaaaattct tcctcttcat aatcttcatc atcatcgtct
     2761 tcgtctatga tacttaaatc ttcttcgata tttcctggca agtcttcttc atcatctgtc
     2821 gggtcatctg ctccgatttc ttgcaagtca gaattataag cagcgatttc ttcatcttca
     2881 tcatcatcgt catcaaacag atcatcgtca tcttcttctg tcagatctgt atcttctggg
     2941 tcatcgtcat tgtaatcgat cgcgtcttca tcatcattga taaaggcgtt gactttcttg
     3001 cgtttacggc gacgtggagt atcttcatcc tcttcgtcca ttccatggtt tacttcttca
     3061 tcgatcgaat cgatagcata ccatgaacgc aagccccaac gattatcccc taatgagata
     3121 aagctgccgt caatattcaa gtcagtataa aattgtgcga gtgaatcacg aatttcactg
     3181 tctgatttcc caagatagtt ttggatctgg ttagctaaat cagaaaaatc catgacatcg
     3241 ccacgttgtt ctaagatcgc gtgtgctact tcgatcattg ataattcgct tttgtttgca
     3301 ccttcaaata cactaatttc caatcaggac acgtcctttc acagtctact ccttatcata
     3361 caaaatcaga cacaaaaaat caaacttttc ttttatattt acgctgtaaa atccaaggtc
     3421 aaataatatt cgccgatttt ttccggtccc atgaacagat cataatcgat tcgaaccttc
     3481 cctgctgtgg gacggtcttt caaactaaca tgcagttctt tcgtaaaagt agcaatctgg
     3541 atcataccgt atggtgttcg ataggatgtt tctaaccgtt ctttatagcc aaatttcaaa
     3601 cgcatccgca tttctcctgc acggatcagc tgtacatgac catctggaac taatttgatc
     3661 gttacaggaa tctcatcgcc cgtctcctcc tgaatctctt tataacgtat gtacagcgta
     3721 tcgccaattg tcaccatttg ccctttcaaa tcaaaataaa aatcttgcac ttccccttgc
     3781 tgttttactt ttgtacgcaa tttgattgaa atcggtgttc cttgtgataa ttccattatt
     3841 cattctcctt tacttctcat agctacattg ttcctagttt cttttgtttt gtcaaccaac
     3901 ttgtccattt ttacagaaaa ccttttttcc aaaacaactc tttgatttta gagtataata
     3961 ttgtagaaaa tatgaattta tacaaaggag ccggttgaat gaaccctgcg attcgactag
     4021 atcagcacat ttatcaaaaa aacgactttt ccccttttgc attaacagat atattaacgg
     4081 aatattctaa agaacaccag caaatctttt tacatttctg gcaatatgac tgcacagtca
     4141 tcttaggtat gaaggatgtt cgaactcctt ttctaagtga cgggatccaa acactggaag
     4201 atgcaggtta cgctccagtg atcagaaatt caggtggatt agggatcgta tcagatgaag
     4261 gaattttgaa tatttctttg attttccccc aaagtgacac aaaaaaaata accatagatg
     4321 acgcttacga gcaaatgctc ctattgacta aatacgcttt tccaaaggca ccgatacaag
     4381 cattcgaaat cacccattct tattgtcctg gaacttatga tttaagcatc catggcaaaa
     4441 aatttgccgg tatcgctcag cgtcggatca aaaatgggat ttctgttatg atgtacttaa
     4501 gtgtcaacgg taatcaggaa aaacgtgggc aagtcatgaa gcgattttat caatctgctt
     4561 taaaagaaga ctttggtaca aatggctatc ctgcagtgga tcctgctaca atggcaacac
     4621 ttgaagaact gcttgaccaa cccctttttg taaaagaagt caaagaagca tttgcaaaag
     4681 cttttgaaaa gctgtattct gcaagtgatt tacaggattc taccgactgg ctaaaaaaag
     4741 aaatccagct ggagcaatgg aacaaccaaa tccagagaat ggaacaacgt aataacataa
     4801 aggagctaca ttatgacaac cccttataaa tatcaaatgc tgcctatgga aaaagttttt
     4861 cgtgatccag tccataacta tatccatgtc cagcatcaag taatcttaga tttgatcaat
     4921 tcgaaagaag tccaacgtct acgacgcatt aaacaattag gcacttcttc tttcactttt
     4981 cacggagcag aacatagccg ttttactcat tcattaggcg tttatgagat ttcaagacga
     5041 atctgcgata tcttttctcg aaatttttca aaagaaaaaa taggcaacgg aggctgggat
     5101 gatcaggaac ggctggtgac tttatgtgcg gcactgttgc atgatgtcgg tcatggtcct
     5161 tactctcata catttgaaca tatcttccat acgaatcatg aagccatcac tgtagagatc
     5221 atcacttctc cagaaacaga agtgtatcag attcttaacc gagtagaaga aggttttcct
     5281 gaaaaggtag ctagtgtgat ccaaaaaact taccccaacc cacaagttgt ccaaatgatt
     5341 tctagtcaga tcgatgcaga tcgaatggac tatttgcttc gggatgctta tttcactgga
     5401 acagaatatg gaacatttga tcttacccga atattacgtg tgattcgtcc ttatgaaggc
     5461 gggatcgctt tttccatgag tggcatgcat gcagtggaag attatatcgt tagccgatat
     5521 caaatgtatg tgcaagtgta cttccatcct gtttcccgtg gtatggaagt aattttggag
     5581 catctgcttc atcgcgcaca cgagttgtat caagaagatg ccagcagttt tgaactccat
     5641 tctcaattac ttctgccatt tcttgacgga agctttactt tagaagaata cctcaaactt
     5701 gatgatgggg tcttgggcac ttactttact caatggtctg aatctgctga tcctatttta
     5761 aatgatttag ctaaacgttt tttgaatcgt aaaccactga aatctgctac tttttctggc
     5821 aaccgggatt caaaattagt ccaagaactc actttgttag tagaaaaggt cggatataat
     5881 cctgtttatt acactgctgt gaattctagc tacgatctac cttatgattt ttatcgccca
     5941 gaacaaggca gacatcgcac acaaatcgag attttgcgaa atgacggaac tttgatagaa
     6001 ttatcacagg ttagtcagct tgttgctgca ttagcgggac aagaacaagg agatgagcga
     6061 tttttctttc caaaagaaat ggttgatccg tccttgcgtg atcattatga tttatttgac
     6121 gaaacttatc aagaatttgc cagccatatc cgtaatggcg cattgattga aataaattaa
     6181 aatatattgg aggaaattat gtctatcaaa ctagtcgcta tcgacatcga tggtacttta
     6241 ttaaatgaga aaagagaagt aacaaaagaa gtaaaagaag cgattgccgc tgctgttgca
     6301 aaaggtgttt cgatcgtttt atgcactggc cgtcccttac caggtgtcca ggaacagtta
     6361 aacgagttga acctttttca agataatgat tacgttatta cttataacgg ggcactcgta
     6421 caacaaacga aatcaggtaa aatcatcgcc cgtcacggac tgacacatga agatttccta
     6481 gagatcgaag taatggcacg ccgtgtcggc agtcatctcc attcgatcga tgacaaagcg
     6541 atctacacgc ctaatcgaga tatcagcgct tatacgatcc acgaagcaag tttagtcaag
     6601 atgccgctaa aatatcgaac acctgaagaa atgacaccag acatgaacat cgtcaaaatg
     6661 atgatgatcg acgaaccaga aattttagat gcagcaattg cgcgtttacc gcaaactttc
     6721 cgtgataaat acacaacggt caaaagtgcg ccctattact tcgaagtact aaataaagaa
     6781 gcaagtaaag gtgcggcagt tgcgaatctc gcacagcacc ttggtattga tcaagatgaa
     6841 atcatggcta tcggagataa cgagaacgac ttatccatga ttgaatatgc tggtctagga
     6901 gtagcaatgg gaaatgccgt ttctcttgtc aaagaagcag caaatgtcat cacttcatca
     6961 aacgacgagc acggtgtagc tgaagcaatc aaaaaatatg tactgtaata aataaaaaaa
     7021 ctgagagaaa gctgatctac tcgctttctt tcagttttta tttttttcga tccatctctc
     7081 gactgcccat cggtggctac cacgtttcaa tttcacaagc gcttcttgag ggctcaccca
     7141 ccaaatttta ttggtctttt cagttggttc tcctatacgc ttccaagaat cagcaacaaa
     7201 gaaatatcct ggattataat aataagttgc acgataattc gagtaaaaat actcatcagc
     7261 ttctcccaga taagaagaaa tttctgccac aattcccatt tcttctatca gctcacggac
     7321 aatcgcttct tctttcgtct cttctccttc aatttccccg ccaggaagaa aaaaagcacc
     7381 atttggagcc tcgattactg caatctgctg atctctttct atgacaatat atgctgcata
     7441 acgggtttga taatcagcaa attgcttttt ttcaccaaaa attggtttat ccatgaatag
     7501 tttccacctt aacagtttta ttttctatcc attttacatg agaaatttct catgaacaag
     7561 cacaaatcaa ctatttcctt acttcttttt gttatgattg agtaaaagga aaaggagaga
     7621 tgaatctatg aaaatggctc acacatgtgt tcgagtgaaa gatcttgaag catcaatcga
     7681 attttatcaa aaggcatttg gttttgaaga aagccgccgc cgtgattttc cagaaaacaa
     7741 atttacactt gtttatttga cacttcctgg ggatgactat gaattagagt tgacttacaa
     7801 ctatgatcat ggtgcgtatg atctaggaga cggctatggt catatcgcta tagcagctga
     7861 tgatctagaa aaattacacg agcaacataa agctgctggt cttgagatta ctgacttaaa
     7921 aggattacct ggcactgcgc cttcttacta ctttgtcatt gaccctgatg gttataaaat
     7981 cgaagtcatc cgaggatagt tagtatgggg cattttactc ttgattgaat aaaaaacaaa
     8041 caccaataat taaaatgaga cgcaaacttt tcattaaatt gaaaaagttt gcgtcttttt
     8101 ttaacaacca ttgtagctat aaggttgcca ctctccacca atttattttt aattcatttg
     8161 aaagaaatca aataaacgag gtacctttag aaaacaaaac tgcttttcct cctattatca
     8221 cacgatcttc ttttaattca cattctagat ccccgcccct tgatgacatt tgataagctg
     8281 acaattttgt tttattcatt ctttctgccc aataaggaat aagattagaa tgtgctgatc
     8341 cacatactgg atcttcattg attgttaatt ttggaaagaa ggttctagaa acaaagtctt
     8401 tttgcacact ttctgctgtc acgatgactc ctattcctaa ttcaaaattt ttgatagctg
     8461 taaaatctgg ctgcaattta gccaccgttt tttcatcttc taatacgaaa aataaatcac
     8521 gtgctaagta ggcttcttta atcttagccc caatcgcttc ttcatattca gacaatatcg
     8581 gcactttttt cggcataata ctaggaaaat ccatgtaata ataatcttta attcttgtca
     8641 caaatagatt accgctttga gtagaaaatt taatcgtttc atcaggtatt ttgtaatagt
     8701 taaataaaac aaatgctgtt gctaatgtcg cgtgaccaca aagatcgatt tctcgatctg
     8761 gcgtaaacca tctaagctca aattcttggt ttttcttcac tgtaaatgct gtttcagata
     8821 aattgttttc aatggcaatt ttttgcatcg taccttctgg cagccattct tctagcacat
     8881 aaaccgctgc tggatttcct ttgaacacct catctgcaaa tgcatcaact acgtaataat
     8941 ctaagttcat tttaaagccc ctttctctaa tttgttataa atatattctg cacaacaaag
     9001 atcttcaata gctagaccaa ctgcgtcgaa aatcgtcggc ctcgattctc ctttataaaa
     9061 tgtttttttc tcagaaacta attcgcctaa tgtaccaaca atacaagatt cctctaaatc
     9121 tccttctgcc atggctataa gcaggtcccc gctttctgtt aaggcagctg agtaatcatc
     9181 tacatagatt tgagaagttt ccatcaacga tgtacttatc tctcttgtat caggagtaaa
     9241 tgtgcccaca gcattgatat gtgcgtcttc ccttacccac tctttttgaa gaaatgcctc
     9301 tttactagag gtcaacgtac aaatgatttc cgctggacgt actgcatctt ctactgatat
     9361 tgcatttata aacgttattt taggatactg ttttttagct ttagcgataa aagtatttcg
     9421 tctttcttct aaaatatcgt aaacaaacac tgtagtgatg ggacgaatgg tagaaatcgc
     9481 agtcaaatgc gacaatgctt gttgaccagc tccaattaaa gcaagtgttt ctgcatgcgg
     9541 atttgctaag taatctgttg ccagcgcaga tacagcagct gttcgaatcc aggtgactgc
     9601 atttgcgttt atcaatgctt tgggtagtcc gttttgagca tcaaacaaga aaatctctcc
     9661 taaatgagaa ggtaaatgat actgatgatt atccggaaaa gcggtaatga cttttgtgcc
     9721 aaaatatctg ttctccccca aataagcagg catcatcgca aacatatttt tgccttttcc
     9781 gtcaggtaaa atttccacca atcgaggatt ttggctgatt ttaccacttt cataatcaaa
     9841 aaaacatttt ttcattactt gaatcgcttc ttcaaaacta aaagaagcta ctacttcctc
     9901 aaaattaata attttcatag acaattccct cctcacaaaa gattaacttc taaaatcaat
     9961 acaatcaatg gtaaaattgt atggtataca attttaagga gtggagaaaa tgcctattaa
    10021 ttcttatgac aattacccgt taacttggcg accagacaaa caaaaattga agcgtcctta
    10081 ttatcgatcg ttgattcaac aattagaaca agatatttta aagaataaat tacagaaaaa
    10141 cactcagtta ccctcccaaa gagagctagc tgattattta gatttaaatt ttactaccgt
    10201 tggcaaggct tataaatatg gaattgagaa aggtttgctg tatacgaata ttggaagtgg
    10261 cacatttatt tctcccaatg tctttgagtc catcaccatc tctaccaatg aagtaaccga
    10321 acatatcatt gattttggtc tggttagttc ttttgaagaa tgtaataaat tcgttgtccc
    10381 gtttattcaa tcaactagta ataacccaaa tcttgttggt ttgcttaatt accaagatcc
    10441 caaaggcaca aattttcaat tgactactgc agcaaattgg ttaaaaactc aaggagtcat
    10501 tccgtcctta gaatctctat ctattgtttc cggtgttcaa aatggattgg ctatcacact
    10561 ttctgcactt ttctctccag gtgatagaat agcaacagac cgatatacat acgctaattt
    10621 cattgaattg gctcagttat atcatttaga gatcgttcca attgattttg acgagcaagg
    10681 aatgcgacct gatatattga ttcaggaatg tagaaaaaag aagattcatg gtgtctttct
    10741 tatgccttct tgtaataatc ctatagggtt tcaaatctct cacaaaagac gagagcaatt
    10801 agccgaagtg attgaaaaag aaggtctatg ggttattgaa gatgatatcc attcattttt
    10861 aacaacttac tatcaacata cgatcattcc tcctttccaa aatttattac ctaaccaaac
    10921 aatctactta gctggaatga caaaatttat ttgttctgga ttgcgagttg cttatcttgt
    10981 agttccagta catgtccaaa gcaagataga acaagcaatt tttaatataa atgtgaaaac
    11041 ttctggatta gatgtggaag tcatcactca aatcctccat tcaaatgaag caaataggat
    11101 cttagaaact aaattaagtc ttacaaaaag agccaatcag ctttttgatt ctcttttcaa
    11161 agtagaaaaa ccaacgaatc gtgtccctta ctatcgaatt cttccagttg atcctgagat
    11221 tgaccctaag ataattgaga atgactttct tgctaacggt atccgacttt atcattccag
    11281 acgctttact acacgtgcgc aaccagatgc attcgttcga atttccctat cctctaataa
    11341 ccttgaaa
//
LOCUS       DAZBSA010000091        11160 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000091 DAZBSA010000000
VERSION     DAZBSA010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.091"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..459
                     /locus_tag="ACHGMT_002230"
     CDS             <1..459
                     /locus_tag="ACHGMT_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835957.1"
                     /translation="VYFQGGNLMIKKLFLLSILTLAMAGCSDSGLATELITKSTEATT
                     EELKCGNDHSDYRGLNRRCMGILGRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCAS
                     YTDVEDAVLLNHVRRCSFMSREVGDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(520..885)
                     /locus_tag="ACHGMT_002231"
     CDS             complement(520..885)
                     /locus_tag="ACHGMT_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFN6835958.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(961..1206)
                     /locus_tag="ACHGMT_002232"
     CDS             complement(961..1206)
                     /locus_tag="ACHGMT_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN6835959.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1276..1530)
                     /locus_tag="ACHGMT_002233"
     CDS             complement(1276..1530)
                     /locus_tag="ACHGMT_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835960.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1694..2017)
                     /locus_tag="ACHGMT_002234"
     CDS             complement(1694..2017)
                     /locus_tag="ACHGMT_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835961.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2204..2277
                     /locus_tag="ACHGMT_002235"
     tRNA            2204..2277
                     /locus_tag="ACHGMT_002235"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2238..2240,aa:Ile,seq:cat)
     gene            complement(2662..3159)
                     /locus_tag="ACHGMT_002236"
     CDS             complement(2662..3159)
                     /locus_tag="ACHGMT_002236"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFN6835962.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(3199..4008)
                     /locus_tag="ACHGMT_002237"
     CDS             complement(3199..4008)
                     /locus_tag="ACHGMT_002237"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN6835963.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            4342..5199
                     /locus_tag="ACHGMT_002238"
     CDS             4342..5199
                     /locus_tag="ACHGMT_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFN6835964.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            5192..6094
                     /locus_tag="ACHGMT_002239"
     CDS             5192..6094
                     /locus_tag="ACHGMT_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFN6835965.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            6127..6477
                     /locus_tag="ACHGMT_002240"
     CDS             6127..6477
                     /locus_tag="ACHGMT_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFN6835966.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            6526..7449
                     /locus_tag="ACHGMT_002241"
     CDS             6526..7449
                     /locus_tag="ACHGMT_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFN6835967.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            7527..8420
                     /locus_tag="ACHGMT_002242"
     CDS             7527..8420
                     /locus_tag="ACHGMT_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN6835968.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            8458..9021
                     /gene="amaP"
                     /locus_tag="ACHGMT_002243"
     CDS             8458..9021
                     /gene="amaP"
                     /locus_tag="ACHGMT_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFN6835969.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            9035..9223
                     /locus_tag="ACHGMT_002244"
     CDS             9035..9223
                     /locus_tag="ACHGMT_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFN6835970.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            9236..10138
                     /locus_tag="ACHGMT_002245"
     CDS             9236..10138
                     /locus_tag="ACHGMT_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFN6835971.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            10170..10412
                     /locus_tag="ACHGMT_002246"
     CDS             10170..10412
                     /locus_tag="ACHGMT_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFN6835972.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(10668..11039)
                     /locus_tag="ACHGMT_002247"
     CDS             complement(10668..11039)
                     /locus_tag="ACHGMT_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6835973.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3942 a         1919 c         2123 g         3176 t
ORIGIN      
        1 gtctattttc agggggggaa ccttatgata aaaaaactat ttttactatc aatattaacg
       61 ctagcaatgg caggttgttc tgactctggc ttagcaactg aactgatcac aaaatcaact
      121 gaagccacga cagaggagtt aaaatgtgga aacgatcaca gtgactacag aggcttaaat
      181 cgacgatgca tgggaatatt aggcagaagc aattgtaagc atcgatgtag taatcgaaga
      241 aggtgtagaa gaagcaacag aaaaatcaaa agaaagatca aaagaagcaa tctgtgctgt
      301 gcatcgtata cagacgtcga agatgcggta cttttaaacc acgtcaggag atgttcattt
      361 atgtctaggg aagttggcga tgaaccaaaa ctagagtacg cttttagttt tgcacgtgag
      421 gatgaagata accctaaaac ttacgacatt ttagcttgat ctaagatggt taaagctttt
      481 taatgaagta agaggagata gaaggctttt ctagttatat taggtaagat aaattccttt
      541 ttgctccaaa tttgctaaac attctgctag ataaacttca tcgtgttccg gataaatcgg
      601 cacgttgcgc tctacagtag gtaaatcaaa aaatgcttcg catgatttgc cacgatacat
      661 aggatttttc cactctggtg ctgggctgta accacgtttt tccatttcat ccatgacaag
      721 ctgatgatat tggaataatc tataaggaga atagtgaaaa acataatcca cagtagcatg
      781 tttctttccc cagccatttc ctcttaatgc acagcattcc cgatgttgtc ctaaaagctg
      841 ttgacgagga agctttgaaa tcaatttttc atgccataat cgcatagaaa tgtctccttt
      901 atatatagat tgttaacctt agacgttaaa tgaaaaaacc agcaccaagg ctggaaaaaa
      961 tcacttcagt ttgcttgctg tttctgagcg accaattagg taatcaacgg aaatatcgta
     1021 aaagtcagcg atatagatta attcaatgat gcttggaatt tcttctcctt cttcgtactt
     1081 acgatatttg aataaaggaa tacttaacgc gctagaaaca gctacttgag ataaagatcg
     1141 gccagttctt aagaaattca gacgacaacg taaagtatcc agtaaaatag cttcttcgat
     1201 actcatttaa tcacctttca ttatagtaga atagttataa tgactataga aagctacaca
     1261 tgcaatcaat agtactcaat gttttttatt taaacgtatc aaataaagaa tgacgatcaa
     1321 taaagacaaa accagtcctg ataattcttc tataaaaaca ctatcgggtt caaagtagtt
     1381 gtaattaaaa actttccaca taaagtagat agccggaaga gtcaaaatag ctaaataaaa
     1441 ccgttctttg ttccaattca taagataaac aatacttgcg accagtgcga ccgcaggcac
     1501 ccaaaacaaa actctcacca acgtaatcat gtaacggact cctcaacgat tcatataata
     1561 aaagataaca tgttattgca tacatcacaa atgaaatgct aggaaaataa aagaagaata
     1621 gatcaatgaa aaatgaaaaa gcagaacagt tttctgctga taagcaggaa ctgttccgct
     1681 gaatgtgtct tatctatcga gaaaaaatca atggattgat atctaagtaa ttcaatacat
     1741 ataaaaagag gacgatcgca ttcactaaaa aggaaagaat gatgatccca tagcgaatgg
     1801 aaacatattt aacaaatact tttgtttgaa tacgggcata tctttttcct ttatagctga
     1861 gcatcatgac aagaagcaaa agggtaatca caccttgaaa aatcattcca ataaacagaa
     1921 ttcctgaggt ttgataattt aattggagaa aataagctgc tgccgcaact aaatcgaata
     1981 aaatcgcaat aaatggcaaa ataatcactc ctaacattga gaataaataa aagaataaca
     2041 ttttttctaa aataagcaaa taaaaattaa agtaacaaaa tagttatgac acaagtaaaa
     2101 gaaataagaa taacaatatt attttactta tattttgaaa cttgtgagag tttgtcattc
     2161 gtgctatagt tgaaaagtaa caaaaataaa ttttatttat gatgtacctt tagctcagtt
     2221 ggttagagca gacggctcat aaccgtccgg tcgtaggttc gagtcctaca aggtacataa
     2281 gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaagtaa aaacagcttc
     2341 ttatgttttt acttggctgt tcttattact gcagatgcaa gcttttgaat accgtttaag
     2401 gaagaggttg tgaaatcagc gttttgacct gagtattagc ttaacaaacg gaaaaatagt
     2461 tcctcatatt ttgccgtttg ttaagctaat accgagggtc agctgattga acaccattta
     2521 agaaagaggt cgtgagcctg ccgtttcact ctgtgtcata aaaaagccaa tctactttga
     2581 tagaaaaagc acgtaggact aaaacagttc tgcgtgcttt ttttattgtg tgttaaatat
     2641 cgtaaatgaa gatagatatt tttatgattc gacgatagag acgttgaaat ctttcaacag
     2701 ctctaaagcg gattctccgt gttttctact ggagactaac attggatcaa caaaaaagtc
     2761 tgcttcagga aaagcactct gcaggcctat cagattagag agtacacaca agtttgtttc
     2821 aatcccgact acttcgatct ctttttgttc ttctttgctt tcaaataaag tttcctggat
     2881 ttcagctaaa gtttcaggtg gaatcgtaaa gtaatacttt ttaatcactc gttcattttc
     2941 ttgtggagaa agaagaggga tcaattgcaa atcttctcgt tcttcttctt tgtccttcag
     3001 ttcaactggt atatcccgtg tgaagagaat atattcatta ttttggcgtg ctttagctaa
     3061 tctttgagca atacgttctg ccaattgatc tgactctgga atataaaatt cgctgtttgg
     3121 atcaagaata tgattttgca tatcaataat aactaacatt ttctttcctc ctacattcat
     3181 atcgtcattt tattcttttt aatagaagtt ttttaaaata taagcaacac cagcttcttc
     3241 attcgtcagt gttacttggt ttgcaatttc ttttacttgt cgctgtgcat tccccatcgc
     3301 aatactcaac ccgctgtaat tcaacatagc aatgtcgttt tcttgatcgc caaaagccat
     3361 gatgtcactc gattcgattc caaggtaatc cgccagaaaa acaagtgaat gagctttagt
     3421 gatacctgta ggcgtgattt ctaagaaatg cggatcagag aatactgatt ggataccatc
     3481 tgcttgcaga tctctttgta gcttctgtag tttttcttca ctcttatctg ttaatgctaa
     3541 tttaagatag ctaagagaag gatcttccaa catatttaaa atttgatcaa aagaatggtt
     3601 ctgtaaattg aaatgttcat aataagggtc atctttgact agatttgatt catcgaatgt
     3661 cagctgatca tcttgggttt ctcggctagc aatgatggat attttcgctt cttgagcata
     3721 gaagaaggct tttctcagtg tatcgctatc tatttttgaa cgtctgagaa ctttcgccat
     3781 ttttccatcc ttcatttcag caatcagtga accattggaa gaaatcaaat agccaccgat
     3841 tcctaattcg gaaaaaatat ctttagcaga aatgctgttc cttccggtag cgacggttac
     3901 gattccgcca ttttcatcaa atttccgaag tgcttggata ttttcttctg gtatctttcc
     3961 atttcgatca agcaaggtac cgtctaaatc aatcgctgct agtttcattt tatcttccta
     4021 ctttcttttt acatatctat tttcctgtta ccttaagatt aaatgagaat ttaaaaaaat
     4081 gaaactaact tgctttgtca ttatgaattt tttcaaaaaa cattcttaag aaaaaggtgg
     4141 ctatatatag tagtttttgg tcaatacctt tgaataaaaa gccatgaaaa acgaatagtt
     4201 agcgcttacc gatgcgtttt gtgataaaaa aattttaaaa tattcaatga aaacaaagca
     4261 tatattttgc tttaaaacca aatgaggaat attctagatg tagaacaggt tagaaaaaga
     4321 ttagaaaaag gagtgatttt gatggagaag agatacggcg tagttcctgt ggtaactgct
     4381 tcagacgaaa attatgctcc ttacttgagc gtgatgatcg caactgcatt agaaaattgc
     4441 aataaaacaa gacgcataaa attttatgtg attgacgatg gtctatcaga atatagtaaa
     4501 gaaggattgg aagaaacggt aaataaatat tcaagtaatg caagtatcca attcctgaca
     4561 gtggaaaaag atatctacga agacttccta gtcagtgacc atatcacaac taccgcttac
     4621 ttaagaattt ctttaccaaa cttattagct aaagaggatt ataagaaagt cctttactta
     4681 gattcagatg tgttagtact agacgatatc gtaaaactat atgatgagcc gttaaatgga
     4741 aaaacaatcg gcgctattat cgatccaggc caagtgaaag ctttggaaag attaggaatc
     4801 gattccgatg acttgtactt caattcagga gtgatggtga ttgacattga tcaatggaat
     4861 aaaaaagaaa ttacagaaaa aactatccat tacctcagtg aaaatggcga tcgaatcatc
     4921 tatcacgacc aagatgcttt gaatgctgta ctttatgaag attgggaaca acttcatcca
     4981 aaatggaaca tgcagacttc gctgattttt gaacgtcatc cagcaccaaa tgaaaaatat
     5041 gaacgtcagt ataaagaagg aaatgaaaaa ccttcgatcg ttcactttac aggacatgat
     5101 aaaccttgga atacgttgaa agatcatccg tataccaatt tatacttaaa aaaattagca
     5161 cacagtactc taacgaaagt aggcgagatc aatgaataaa aaagaaatag cagtagtcgc
     5221 aagttgtaac acaaaattcg taccacattt agctgcatta tttgtctcag tattagataa
     5281 ttgcaacccg tctaaatttg ttcggttcta tgtgattgac gatgatatcg attttgaaag
     5341 taaacagctt ttacgcttct cagtgaaaaa cgcacgaatg aactcagatg tggaattctt
     5401 gaaaatcaat aaagaattct ttacaaatgt ggtaatcagc gatcgtattc cagaaacagc
     5461 ttattatcgt attgcgattc cggaattgtt ccgcggcaca gaagttgaac gtatcttata
     5521 tatggattgc gacatgatcg ctttacaaga tatctctaaa ttatggcgtc ttgacttcgg
     5581 tgattcaatc gtagcagctg ttgaagacgc tgggttccat caacgactgg aaaaaatgaa
     5641 aatttcagct aaatcaatgc gttatttcaa ctcaggtttg atgctgatca atgtgaaaaa
     5701 atggttagat gaaaatatta cgcaaaaagt attagatttc atcgaacata atccagaaaa
     5761 attacgattc catgaccaag atgcattaaa tgctatctta catgaccgtt ggttaccact
     5821 tcatccgcgt tggaatgcac aaggctatat catggcaaaa gcaaaaaaac atccaacagc
     5881 agcaggtgaa cgtgaatacg aagaaactcg caacaatcct tacattatcc atttttcagg
     5941 acatgtgaaa ccgtggagca aggattttga agggccaacg aagaaatatt acgaaaaata
     6001 tgcagggatg acggctttcc gttgtgtagc taaattccct aaatatccaa aatatgcaaa
     6061 agttcaacca aaaacagatg tagttgttaa ataatagaag accacaactg aagaggggga
     6121 gtaggaatgg aaattacaat gtctaaaaat gcagtagaga cgttaattga aaaggttggg
     6181 gaaaatacaa aaatcgcact cgcgcttatc aatgattccg atccatttct aagggacaaa
     6241 ggtgcttttg caaaaggcag cttttttcaa atcattcctt ttgtttcgga atttggagaa
     6301 tatgcgacta aaatcgagca tccattattg gacatctata catctaaatt agagcaaaat
     6361 tatttcggta aacgattgaa catggacttc aacaaacagc ttgacagctt ttcattagaa
     6421 aatgaaatcg cagtactcga ttataatatc aaattaaaga actgcttctt cagctaatag
     6481 tttcgagaaa gtccaaaaag gaggtataca ttgagatact actttgtgga tgaagaaaag
     6541 aatgtaatag aaacagatga aaaaaaatat aattggctcg tacttgatag tgcaaagccc
     6601 gaagagattg agcaagcaat cgaagaattc gagcttcctg atgatatttt cgtaggaacc
     6661 gaatatccgg aagaagtttc tcggatggag cgcctttatg agacgaaact agagcatcca
     6721 ttttctttgg tagtgattaa tctagatcag tcagataatc gtatcgaaaa aaaattaacg
     6781 ccaatatctt ttgtactttc cgataatctc ttgattactt gtatcgatag aaaaaccgaa
     6841 tttgtggatc ggctgctgac aaagcatgga aatcatttaa actcgtttga aaagatcatc
     6901 acgtatacat tattagatat ttacacccat tacgtgaaag aactaagaga gatgaaaaag
     6961 cgaattgacg ctttggataa agcagctcgt aaaacaacgg aaaatgaaga actgtataaa
     7021 caggcagatc ttgaaagaga catcgtatat atcgatcata ccttaagaga tcaacgggaa
     7081 acaatggatc aattatggaa ttctgaaact ttcatcaaac gtttagacga tgaacagcta
     7141 ctctatgatg tccatttgag acagcgacaa acagaaaaaa tgatcgagat ctatcgagat
     7201 ttattagaga gtattggtga tctattcaac gggatgatgg acaataatct aaatcatatc
     7261 atgaaatatc tagattcagc tgcattaatc atttccgttc cggcgttgtt cgcagggatt
     7321 tggggaatga atacaggagg attaccgggt aaaagttctg ctctaggctt tatactcgta
     7381 gtctttggct ctttgatagc agcagtagtt ttaggttacc atttataccg gaaagattat
     7441 acaaattaaa gaatctattt aaaacaggta tcaaacaaag aaatttgttt tggcaataca
     7501 atttaaaaac taggaggaat acagcaatga cagaaccaaa attaatcgac ccgcgaaact
     7561 tgtaccatac ggagaaattt cctaaacaag atcaggatac accagcttta cagcagaata
     7621 tgattcctgt tcctgattgt ggagaagaat cctatgaagg caataatcaa ctagaaaacc
     7681 gccgtgtact aatcactggt ggagattcag ggatcggtcg tgcagcagcg attgcatttg
     7741 ctagagaagg ggcagatatc gctcttcaat tcttcccagg agaagaaaaa gatgcagagg
     7801 aagtagcaaa atacgtgaaa gatgcaggac gtaagattgt cttactacct tttgatcttc
     7861 gtgacgaaca agcacctaaa gaaatcatag ataaagccgt gagtgaactt ggcgggctag
     7921 atacgatggt actaaacgca ggacagcaaa tttcttgtca gtctattgct gagttaccac
     7981 ttgagcaagt caaagacaca tttatgataa acatcatcgc tatgtttgct ttagtgaaag
     8041 aagctgttcc gcatatccca gcaggaggag caatcgtaac aacaacttct gttcaagcat
     8101 tcaatccaag cgaacattta ttagattacg cagcaacaaa agcttcaatc gctaacttta
     8161 cgatcggttt agcaaaacag cttgcaccta aaggaatccg tgtaaatggt gtagcacctg
     8221 gtccaatttg gacaccatta caactagacg aaggacaacc ttctgattcg atccctgaat
     8281 ttggtcagca aagtttacta gaacgtgcag gtcagccagg agaattagca ccagtttatg
     8341 ttttcttagc atcgaataaa gcaagttatg ttacagccca aatttacgga gtaactggtg
     8401 gagaagcgat caacttataa gaagttgatc gtttcacccc tatgaggagg tgcgaaaatg
     8461 aataaaggtg taaaaacgat cggagtgatc ggaacattac ttttgctatc tgtgcttttg
     8521 tttgcgatac tattaagtgc aacctatcca ttgccttttc agctagagaa gtttcgattc
     8581 ttttccatca ctaattcata cgtgcaacaa tatgtatttt ggcttgccgt tgcatttgct
     8641 attttgacaa ttatcacgtt acttgttttc ctgttttatc caaaaacaat cggaaacttt
     8701 ctactgaaac atggtgatgg ggagttgacg ttggataaaa aagcaattga aggaatggtt
     8761 cgttctcact tgcatgaaga tgaattcatc cattcaccaa aagtaaatgt caaagcaacg
     8821 aaaaacaaga tcgccgtcaa tgtaaaaggg gagttgaaac gaacttcagc actcattggt
     8881 aaaacaggaa cattgatgaa agaaatcgaa aatgaagtac aacacatatt gggaacaaaa
     8941 gagccagtga aagtatcggt gaaatacaca ggctttgaag aaccagctaa acgaagcaat
     9001 gaccgcaaac gagtagaata gggggggacc aaaaatgaaa gaactatttt cgacatacaa
     9061 gctccctatc atctttgggg tacttggatt gatattagcc gttttatgga tcaccgtggg
     9121 ctttttcaag accttattga tcttgatcat ggcagcgatc ggcgtcgcta tcggttttta
     9181 tctgaaacaa acgaaaatct tagatcacta ttttaaatct taggaggaaa catccatgaa
     9241 taacaataac aaaaataata acaataaaat gaacaaccaa aaaccagtgt ctgcacatcc
     9301 agacccaacg acaccaaacc aatcaaatgg tgtgaacgat gaaaagaaca aaacagcacc
     9361 agtatctgca catccagatc cagtagtccc taacgttcaa caaggagtag aagagaagaa
     9421 acatgatgca gaaaagcatg cacctcatca tccaggaact ccagaatcta aagttccaga
     9481 acaacatgat gcaatcgatc cttctaaacc tgtggaagag caaaaacatc cttctgacca
     9541 tgcgaaagca caagaaaaac ttagttctga ccacacaaac ggacaaggaa aacttggttc
     9601 tgatcataca aataaccaag gaaagcatga ttctgaacag gaagattcaa tgcgcggtga
     9661 gctgaattat gaagataaag ttgtgcaaaa aatcatcgga atcgcaattg aacaagtaga
     9721 cggtttatta tctgcaaacg gtggtttctt ctctaatgtg gcaggaaaac ttgtgaatac
     9781 agataatgta acggctggga tcgaaacaga agtcggtaaa aaacaagtag cagttgatat
     9841 ggatgtcatt gtggaatatg gcaaagatat cgaaaaaatc tttgaagaaa tgcaagaagt
     9901 aatcggcaaa gaagtaaaaa atatgacaca tttagaagtg atcgaagtca acgctaacgt
     9961 agtggacatc aaaacaaaag aagaatttga aaaagatcaa gaaacggtgc aagacaaagt
    10021 aactgacgca gcgaaaaaaa ccggtgaatt tgcttcgaat caaacagata aagtaaaatc
    10081 agcagctgga agcggcgcac aaaaagtaaa agaaaataca gaaccacggg ttcagtaata
    10141 aggaagtaaa acattaggag gaacgaaaca tgggatttat ttggtcactt atcgtcggag
    10201 gaattatcgg agcaattgca ggagcaatca cgaataaagg ttcttcgatg gggattattg
    10261 caaatgtcgt agcaggttta gttggatcag cacttggtca agcattatta ggttcttggg
    10321 gaccaagtat tgcaggaatg gcaatcgtgc catcattgat tggtgccatc attttagtac
    10381 taatcgtatc attctttgct cgaaaactgt aaaaagactt ttaaaaagag gtcgtgaaaa
    10441 agctgttctt catcaagtaa taagaacagc caaacaaaaa tagcttctca tatttttcat
    10501 ttggttgggc atattatcga agatgcaagc ttttgaacac tgtttatacg gaataagagg
    10561 ctgtgacaag acttttgtca cagcctctta tttatgcggg caaattccca attagatttt
    10621 taaaagtttt tccttaaatg tgtctttatt gatacagaga agcttcttta ataaaacaat
    10681 gcctccctaa attttcttga gataggaaag gtgaacatac aaaataatac gaccactttg
    10741 aaaacccaga tgatccacaa atggatcgta tgatactgat gaagtaaaaa gcaatggatc
    10801 agaaactcaa agagattgaa aaagacaagg aagaaataat atctcttttt tactcttgtc
    10861 tgttttagcc aataatagct cgtaaagtaa tctagacaaa caaatagaag taaacaaagt
    10921 ttcgtaagag cagcaatcgt atcatgttgc tctgctagac gattgacgag caggaaaaat
    10981 gttggtaatg gaatatagat cacgagtaat gaaagaaaaa agtaaacaat aaatggcata
    11041 ctattcctcc taattgtgtg agttcgccct cattttaaaa taacgatatt tgtttgtgaa
    11101 gctttttcgt gtcgtaacaa atgttatcta atgtattttt aaataaataa tcattattat
//
LOCUS       DAZBSA010000092        11123 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000092 DAZBSA010000000
VERSION     DAZBSA010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.092"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..1344
                     /gene="lysA"
                     /locus_tag="ACHGMT_002248"
     CDS             85..1344
                     /gene="lysA"
                     /locus_tag="ACHGMT_002248"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFN6835974.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1331..2131
                     /gene="dapF"
                     /locus_tag="ACHGMT_002249"
     CDS             1331..2131
                     /gene="dapF"
                     /locus_tag="ACHGMT_002249"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFN6835975.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2134..3015
                     /gene="dapA"
                     /locus_tag="ACHGMT_002250"
     CDS             2134..3015
                     /gene="dapA"
                     /locus_tag="ACHGMT_002250"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFN6835976.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3012..3728
                     /gene="dapB"
                     /locus_tag="ACHGMT_002251"
     CDS             3012..3728
                     /gene="dapB"
                     /locus_tag="ACHGMT_002251"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFN6835977.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3734..4429
                     /gene="dapD"
                     /locus_tag="ACHGMT_002252"
     CDS             3734..4429
                     /gene="dapD"
                     /locus_tag="ACHGMT_002252"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFN6835978.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4445..5446
                     /locus_tag="ACHGMT_002253"
     CDS             4445..5446
                     /locus_tag="ACHGMT_002253"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFN6835979.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5443..6804
                     /locus_tag="ACHGMT_002254"
     CDS             5443..6804
                     /locus_tag="ACHGMT_002254"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFN6835980.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6764..7915
                     /locus_tag="ACHGMT_002255"
     CDS             6764..7915
                     /locus_tag="ACHGMT_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFN6835981.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7890..9059
                     /locus_tag="ACHGMT_002256"
     CDS             7890..9059
                     /locus_tag="ACHGMT_002256"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFN6835982.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9234..10613
                     /locus_tag="ACHGMT_002257"
     CDS             9234..10613
                     /locus_tag="ACHGMT_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN6835983.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10894..11123
                     /locus_tag="ACHGMT_002258"
                     /pseudo
     CDS             10894..11123
                     /locus_tag="ACHGMT_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3702 a         1945 c         2493 g         2983 t
ORIGIN      
        1 ttaatattat tccaataaaa ttcaaatctt tatcaaatga aattccaata aaaacggaaa
       61 aaaagtgaaa agaggaatga aaaaatgaca gaattgataa ttggcggagt cacagcaagc
      121 acattggcag aaaagtataa gacacctctg tatgtgtatg atgaagaaaa gatgagacaa
      181 acgatgctgt cattcaagcg aggctttgta tcgaaacgtt ttgatacaaa agtgctttac
      241 gcctctaaag cattccaaac gattgagatg ctgcatttag ttcgtgaata tggattcggg
      301 ctagatgttg tcagtggtgg ggaaatctat accgctttgc aagctgattt tcctagagat
      361 actatctact tccatggtaa caacaagacg ccagaggaac tgatctatgc acttgaaaat
      421 gatttgttgc atattgttgc cgataatttg atggaagttg aattattagc agaattaagc
      481 caacgctatt taaaaaaaat ctgtatcatg cttcggttga atgtcggagt agaagcacat
      541 actcatgaat atatcgtcac tgcgcatatt gattcaaaat tcggcatgag ctatgaaagt
      601 aaagagtgcc aacaagccct tcagttgata aaagaaagca gctatctaga gttagaagga
      661 tttcatgctc atatcgggtc acaaattttt gatgttactg cttggttagc tgaaatcgac
      721 aaattgacga attacttaac cgattttgac caacctcttt ccttgaatct tggcggaggg
      781 tttgggattc gttatacaca aaatgatcag ccgatcccag tcgaagaggc actaaaaaaa
      841 ctgattgaat atacggaaga agctttggaa aaacgaaaac tgaccattcg tcaattactg
      901 atcgaaccgg gtagaagtat tgttggagaa gctggtacaa cactatacac gattggtttc
      961 atcaaaaaga caccacataa aaaatattac tttgtagatg gcgggatgac agataatatc
     1021 cgtccagctc tgtatcaggc agaatacgat tgtgatttag cagcaaaact gtcggaagaa
     1081 aaaacagaga aagtgacgat tgcaggaaaa atgtgtgaat caggcgatgt ggtaattaaa
     1141 gaagcttatt tgccgaaagc agaatcgggt gatcttttag ctgtttattc cacgggagct
     1201 tatggatact cgatgagcag caactacaat cgagcgacac gaccagcagt tgtttttgtc
     1261 aataatggcc aatcaagact gattgttcgc agacaagcat tttctgatct actaaggggg
     1321 gaagtttcct atgatccttc ataaatataa tggctgcgga aatgatttta tcttattaga
     1381 ttacgcagaa gacacagatt atagtcaatt agccgcttct ttatgcacaa aagaaaaata
     1441 tgatatagac ggattgatcg ccgtaaaaat cgatccgttg gaaatgatct attacaacaa
     1501 agatggcagc cgcgcgccga tgtgcggaaa tggcattcgc tgttttgctc gatacgtaaa
     1561 tgatcagaac atgatgaatg aacaggaatt caacgtagag acattagcag gcacaagaaa
     1621 aattacggtg ttggatccct caccttttta ttgcagcgta gatatgggag tgccagatga
     1681 ttctccggaa aaaatcaaag ctaatcaaac ttatccaata aaaaaccagg aaatagagat
     1741 caatggtgaa aaagtaacga tcactagttt atttttagga acgattcata ccgttgtttt
     1801 tgttgaaaat gcgcgtgagg aagtctggag aaaaagaggt gaactgatct gtcatcatcc
     1861 tttgttcaaa gaaaaaacca atgtcaactt tgtagaagta gtcaatgaac aagaattgat
     1921 cgtccgtacc tatgaaagag gagtaggttg gacacttgct tgcggtacag gttgctgtgc
     1981 ctcttatgtg gttgctaagg aaactgggaa aatttcagca gatcaggtag ttgttcactt
     2041 ggaacaagga gacttgatga tctctggtga ggatgcgata caaatggctg gcccagcagt
     2101 tcatgaatgg acaagaatat tggaggaatg aagatgataa aaggttcgat cgtggcgctg
     2161 atcacgccga tgaatgaaga gggatcagta gattatgctg gtttggaaaa attgatccaa
     2221 ttccatttag atgaacaaac agatggatta ttagttttag gtacaacagg agaatcttct
     2281 acgctcacac aatctgagga ggagcagatc cttcaactga cagtaaagaa agtggctggc
     2341 cgtgtgccgg tgattgcagg agctggaacg aataatacga aagaaacgat tgaaaaagcg
     2401 aagcattttg cttctttggg ggcagacgca cttttggtca tcacccctta ctacaacaaa
     2461 acaagtgatg caggactagc tgctcatttt acagcaattg cagaagcctc accgattccg
     2521 cttattttgt ataatgttcc ttctagaaca ggcatgtcga ttccaatcca tgtattagta
     2581 aatttagcag aacatcctaa tatcattggt ttgaaagaag cctcaggaga tatggcttat
     2641 gtgatggatg ccgctcggtt gattggagaa gaattcttcc tttacagtgg aaatgatgat
     2701 ttgatcctac cagtaatgag tgtaggagga agcggtgtaa taagtgtatg ggcgaatatc
     2761 caacctaaaa tagtccatga attagtaaaa gatacgcagg acggaagatg gcagcaagca
     2821 aaagaaaagc aattaaatgc acttgaactg atccatgcct tgttcagtga gaccaatcct
     2881 attcctgtca aagcagccat gtcattatta gacttacctt ccggacctct acgcctgcct
     2941 ttagtttctc tttcaaaaga gaaaaagaaa caattagctc agcttttgtt aaaagaaagg
     3001 acggttgaaa aatgaaaatc ggactgattg gcagtggaca gatgggaaat agagtcgaag
     3061 aagttgcaca agaaagagga gatacggtcc ttcttttacg gacggcacct aaagaagcaa
     3121 ctacgcttgc ttttacggaa ctgccagaac tgcttattga tttttctcat ccagataatt
     3181 tagagaaaat cctttcgtat agcttatcat atcaacttcc gttagtcatt ggcacgacag
     3241 gttatacaca atcacagttc caagaaataa aagaacatgc caaacatgta cctgtgatgt
     3301 atagtgctaa tttttcgttc ggcatcatgg tgatgaatca actaatcaag caagcagctg
     3361 caatgttgca aggctggcag atcgaactga ttgaaaaaca tcatagtcgt aaaaaagatg
     3421 caccatctgg tactgcaaat atgctgcttc aaacaatcca agaagtgcaa aagctgcagc
     3481 ccgtatacaa ctggcaaggg aaaaaaagag aagaaaacca aattggtgta cacagtattc
     3541 gtgcaggttc tttacctggt gaacatgatg tattgtttgc tgctactgat gaaatattta
     3601 cgatcaaaca tgaatctttt tctaatcgaa tctttgctgc aggcgcagta gaagcagcgg
     3661 actggttgaa agatcagtca ccaggttatt acaaattaga agatttatta atggacaaag
     3721 gggtgtaaaa aacgtggaag ctcaagaaat catccgctac attcaaacgg catctaaaaa
     3781 gacgcctgtg aaagtctatc tgaatgtaac cgagccaatc gaatttaaag aaagtaaagt
     3841 ttttggtgaa ggaagcagct tcgttgtttt tggtgactat gagacgattg ctccggtttt
     3901 agaagctgaa tcagaaaaaa tcattgaaat cgaaatcgaa actgctgcaa gatacagcgc
     3961 agttcctttg ctagatatta aaaaaattaa tgctcgtatc gaaccaggtg ctattatcag
     4021 agatcaagtg tcaatcggca acaatgccgt gatcatgatg ggagcgatta tcaatattgg
     4081 tgcagtaatc ggtgaaaaca cgatgattga tatgggtgct gtcttaggtg gacgagcgac
     4141 agtgggcaaa aactgccata ttggtgcagg agctgtctta gcaggtgtga tcgaaccagc
     4201 atcggccaaa ccggttatcg tagaagacgg cgtactggta ggtgcgaatg cagtgattgt
     4261 tgaaggtgtc catatcggaa aagatgcagt cgtggcagca ggagcagtgg ttttagaaga
     4321 tgtggcagca gaaactgttg tcggtggaat acctgcacgt gtattaaaaa taagtgatga
     4381 tcagacaaaa gaaaatacag cattgattgc tgcgttacgc gaattataga ggagatggag
     4441 aaaaatgcgc gaaatagata cctgtattgt gggggcaacc ggattagtcg gccaaacgat
     4501 gctcaaagtg ttagaagaat atgattttcc agtaggacga ttgaaattat tagcttcaaa
     4561 acgttcagcc ggtaaagttt gtgtattcaa gaaaaagaaa cacactgtac aggaattgac
     4621 agaaacttca tttgaaggat atgatttagc gctattttct gcaggtgctt ctgtatccaa
     4681 agagtttgcg ccaatcgcac gaagtcaggg actatttgtc atagataatt cctcagcatg
     4741 gcgggaagat tcggatattc cattaatcgt tccagaggtc aatctggaag actatacatt
     4801 gaatcggctg attgcaaatc caaattgttc aaccattcaa gcggtacttc cgcttaaagc
     4861 tttacaagaa gcctttggta tccagcgagt caattattcg acctatcaag cagtttctgg
     4921 ttccgggcaa aaaggcattg aagatcttca tcgtacgcaa aatgacgagg aaccagtttt
     4981 gtatccttat aatattagcc aaacggtgat tccggaaatc gatcagcctt tggaaaatgg
     5041 gtatacaaaa gaagaacaga aaatgataaa tgagacccgt aagattctgc atcagcctgc
     5101 tttgccagta tcagcaacct gcgtacgtgt gccaatcgaa aatggacatg gagtctctgt
     5161 ggcagttcaa ctgcaaaaag agtttacgac ggaagaaatt agagagtgtc tcagccagtt
     5221 tccagggatt caattagaag atgatctagc agcacataaa tatccgacga gcattcttgc
     5281 tagaggaaca gatatggttt atgttgggag gatcagaaaa gacacttctt tagagaacgg
     5341 attgttgttc tatacgactg ccgataacat acgcaaagga gccgctgcga atgccgtaca
     5401 gattgctgca gctttaatgc agaaaatagc ggaggtggca agatgagtaa tacgctgaaa
     5461 gtagcgaaat tcggcggaac atcgatgtct catgaagagc aattcaaaaa agtccgtaca
     5521 attgttcttt cagatcccaa tcgccaagtg atcgttgttt ctgctttagg caaacgtacg
     5581 tcagatgatg acaaagtaac agacttactt tatttgattt atgcttacct tcagcatcat
     5641 gttgattgga cacccctttg gaaaaggatt tgtgatcgtt tcattcaagt aagagatgtt
     5701 cttgccctgc agttacctat tgaagatgaa ctagcacgca tacagaaaca actggaagac
     5761 gaaaagataa ctgaagatta tttagtaagt agaggtgaat atctgactgc tcaactgatg
     5821 gctgagtatc ttggttacca gtttattgac gcaaaagacc tgattttctt tgactatgat
     5881 gggaaaatcg atcaggaaaa aacagcacag gaaatcacaa aagcatttaa aagatatccg
     5941 aaaatggttg ttccagggtt ttacggggcg aaccctgcag gaaaagaaaa attattgggc
     6001 cgtgggggaa gcgacattac cggcgcgata ctggcgaaga tgttgcaagc ggaaagttat
     6061 gagaattgga cggatgtttc aggaatcatg atggctgatc cgcgaatcat cgatcatcct
     6121 aaaatcatcg aggaaatctc ttttagagaa ctgagagaga tggcttatat gggggctaac
     6181 gttcttcatg aagcagctgt tttcccagtt caagaggctg atatccccat ccaaataaaa
     6241 aataccaatg atcctttcgc tcaagggaca aaaatatcga atcacgaaat agaaaaggaa
     6301 aatggactga caggaatagc tggacgcaag gattttcttt ccatcacttt attcaaacgt
     6361 catatgtccg atgaaatcgg gtttatctgg aaagcaatga gcatttttgc taaacatgag
     6421 atatcaatcg aacatattcc ttcagggatc gacaatatcg gagtgatcgt ctcagcagaa
     6481 gcgattgccg ataagttatt tctcatcacg aaagaattaa aagaaacact tggcgtggaa
     6541 gaaatcgtcg tgatcgaaga cttagctttg atctctgtcg ttgggggtcc gcataaagaa
     6601 ttgattggat tatctgggaa agtgttatcg atattgaaca aattagaaat tcgcacatcg
     6661 atcttatcac aaggagcaca ggagttgaat ctgatcatcg gtgtaccgaa taatcaatat
     6721 gaaacagtag tgaagggtat ctatgaaggg atggtgaata cggatgctga aagaatatcg
     6781 aatggcactc catcaaattc ctgaaatcgg gtttcatgaa ttcaagacaa aaaaatattt
     6841 atatgaacaa gtaaaaaaca gcgaggccgt tatccatgaa atcggcgaaa caggactact
     6901 cctttatttt gataatcagc aagaagaaac gattgctttt cgaacagata tcgatgcact
     6961 gccagtcaaa gaagaaacga atctctcttt tgcttctaag catgaaggtt atatgcatgc
     7021 atgtggtcat gacggacata tggcgatgct tttagggttg actgacttta taactaaaca
     7081 cattgaggat cttatttata atgtagtact gatctatcag ccatcagaag aaatttccgg
     7141 tggcgcacaa agcgttattc agtcaggatt gttggataaa tatgaagtac aagcaatatt
     7201 tggctttcat ctatggcctg gactagctaa gggagaagtt ttctctcgac caggaccttt
     7261 gatggcacaa agcagtgaaa cagacattat cattaaaggc cagtctgcac atattgcttc
     7321 tagtgaaaaa ggaatcgata gtctggaggc agctgtacga tttttgaaga atgtttatga
     7381 ttttgaccgg caattaccgg cagagtggaa tcatctaatg aaatttggcc aaatccaagg
     7441 tggaacgatc cgaaatgttt tagctggaga agttattgta tccggaagta tccgttctta
     7501 cagttcagat atgcaaaatt atttgaagag gggattagac catttagcag aacagtttca
     7561 ctctacagct actatttctt ttcgctataa tgacggctat ccagctgtaa tcaaccatag
     7621 aggcttatat gcagcactgt tagattcaaa caaactacat gaattagatg ctccagttct
     7681 tcaagccgaa gattttggca tatatacgca acaatatcca tgtgtatttt tctttttagg
     7741 tgtcggtgat acccctgctt tacatgatcc gacgttcgat tttgatatgg atgttttaga
     7801 aaatggtgta gaatggtaca aaacgattct ttttacagaa aatcttcttg taaaaatcga
     7861 tagcagttca aaagaggagc gagtaaataa tgatgaattt atccaatcga tttagtccta
     7921 ggctttctag aatagaagta tcaaaaatac gacaattcga tcagcaaata tcgtcgatac
     7981 cagatgtgat caaactcacc ctgggagaac cggattttcc aactcctgaa catgtcaagc
     8041 aagcaggaat agcagcgatt gaagaagact tttctcacta taccggaatg agaggattag
     8101 aggaactgcg ggaagcggct tgtatatttc agcaacagcg ttatggatta acttatgatc
     8161 cgcaaacgga agttttgacg acagttggtg caacggaagc gattgcctct gcgttgttat
     8221 ctgttttgga agaaggagac aaggttctta ttccagcgcc tgcttattca ggatatcagc
     8281 cattagtgga actagctggt gctgaattga tcccgataga tacttctgac acagggtttg
     8341 tctgtcagcc ggaacaattt gaacgagcat ttgaacaata tggatcagct gtcaaagcag
     8401 tcattttaaa ctatcctaac aatcctactg ggacgacatt gtctgcaaaa caattgaaag
     8461 caatcgctga ggttttaaaa aaatatccgg tatttgttat aagtgatgaa gtatatgctg
     8521 aattgactta tagtggaacg catatgtcca tagctactta tcttccagaa caaacaatcg
     8581 tgataagcgg gttatccaag tcacatgcga tgactggttg gcgagtgggc tttatttttg
     8641 ctcaaaaagc ccttattgac gaactaatta aagtccatca atatctggtg actgccgcta
     8701 caacgatgtc gcaaaaagct gcggctgaag cgctaataaa tggggtgaaa gatagcgaga
     8761 atatgaaaga gcaatatcaa acacgaagag actatctggt ccagcaattg gcgcctttag
     8821 gatttgaagt gacccagcca aacggtgcat tttacttgtt ttgcaaactc ccagctgcca
     8881 ttcaaactga ctcttggcag ttttgtgtag aattagcgga acaggcaaaa gttgcgtgta
     8941 tcccaggaat tgcatttggc ccagaaggag aaggttatat ccggatttct tatgctagca
     9001 gcatggaaaa attacatgag gcctgcaaac ggatcaaaga ctttttaaca gaacagtgat
     9061 tcatagattt tatcaaaaaa acaatcgtta attttgcggt atattctgca aggagacgat
     9121 tgttttttaa atttagttca aatgaacgcg tttcttttga ttcataacta atgtaatgtt
     9181 aagtttaaat tgtttagaat gattttaaaa gaatattcaa ggaggtttca tcaatggaaa
     9241 aaatcaaagt tgtcattgta ggtgcatctc atggagggca tcaatcgatc ttggaactat
     9301 tgacacgata cggtgaaaat gtagatatca cgctatttga agcaggagac tatatttcct
     9361 tcatgtcatg tggtatggag ctgtatttag aagacaaggt aacagatgta aatgatgtac
     9421 gtaatttcag accagaaaac tttccgcaat ccaatgttca tattttaaat aatcacgaag
     9481 taaaaacgat cgatgccgat aaaaaaacag taacggttac tagcgcatca gataacgaaa
     9541 caaaagaata tccttatgac aaattgattt tgagttctgg tgtcaaacca aacaatctgc
     9601 cagtaccagg agcagatcta gaaaatgtct acttgatgcg tggatacaat tgggcaacca
     9661 aaatcaaaga aaaattaaat gatccggcta ttaaaaaaat cgctgtcgta ggtgcgggtt
     9721 acattgggat cgaagcagca gaagcaagcc gaaaagctgg gaaagaagtc gtattgcttg
     9781 atgtgatcga tagaccttta ggcacttatt tagatgctga gatgacagat attttagaac
     9841 aacacctaaa tgaaaacggt attgaagttt tgacaaatgc aaaaatcgaa gcttttactg
     9901 gtaacggaaa agttgaggca atcaaaacaa gtgaaaaaga aatcccagca gatctagtca
     9961 ttcaagctgc cggtgtgaag gcaaatacag aatggttaaa agggatcgtc gacttagatg
    10021 aacgtggctg gatccaaaca aatgaatact tgcaaacgaa tcttccagat gtgtatgcag
    10081 taggagatgc tacacttgct tattctatcc cagcacgcaa aaaaatgccg atcgctttag
    10141 cgactgttgc acgtagagaa gcacgttatg tagtaaaaca tctttttgaa gaagtaccaa
    10201 acaaaccgtt cggcggagtc gtaggttcct ctgcattaag tgtctttgac tatcattttg
    10261 cagcaagcgg attgaatagc tttacagcag caaaagcagg agtagatgta cgagtgtctt
    10321 attatgaaga tacgttacga ccaaaatatg taccagaaga acaaggaaat ccaaaagtgt
    10381 ccatccaatt attctcggat ccaatgaccc atcaattgct aggaggagca gtattatcta
    10441 cctatgatgt tacaggtcaa ggaaatgtat tggcactagc gatccaaaac aaactgactt
    10501 tagaagaact agcagaagct gacttcttct tccagccagg ctttgaccga caatggagca
    10561 tgttgaatct agccgctcaa caagcgttag gagaagaacc atttgtagaa taagaaaacg
    10621 aaatatttga aaagaggttg tgaaaaagct gccttgcatc aagtaataag aacagccaag
    10681 taaaaacagc ttcttatgtt tttaacttgg ctggacttat taccgcagat gcaagctttt
    10741 gaacaccgtt tattttcaaa aaagggactg tgacacgatt tgtgtaccga cccccaaaag
    10801 ttagaccaaa aaatctaact tttggaggtc ggtatttttt atggttaaat atacttttga
    10861 acttaaatta aagatcgttc atgattattt ggatggcaaa ggcgattgga ccgtaaaaat
    10921 ccagatcaag agattgaagc ggaaatgctt aaaattcggg agaagcacaa ggattatggc
    10981 tgtttacgga tgaccaatga actaagaaac cgcggtttct ctatcaataa aaagaaagtt
    11041 cagcgcttga tcaagaagct aggaataaaa gtaacggcat acacgcgtaa atcacgtcgc
    11101 tacaattctt atcgaggaaa agt
//
LOCUS       DAZBSA010000093        10689 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000093 DAZBSA010000000
VERSION     DAZBSA010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.093"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(124..681)
                     /gene="frr"
                     /locus_tag="ACHGMT_002259"
     CDS             complement(124..681)
                     /gene="frr"
                     /locus_tag="ACHGMT_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFN6835984.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(684..1406)
                     /gene="pyrH"
                     /locus_tag="ACHGMT_002260"
     CDS             complement(684..1406)
                     /gene="pyrH"
                     /locus_tag="ACHGMT_002260"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFN6835985.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1542..2423)
                     /gene="tsf"
                     /locus_tag="ACHGMT_002261"
     CDS             complement(1542..2423)
                     /gene="tsf"
                     /locus_tag="ACHGMT_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFN6835986.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIEYDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2522..3304)
                     /gene="rpsB"
                     /locus_tag="ACHGMT_002262"
     CDS             complement(2522..3304)
                     /gene="rpsB"
                     /locus_tag="ACHGMT_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFN6835987.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3663..4142
                     /locus_tag="ACHGMT_002263"
     CDS             3663..4142
                     /locus_tag="ACHGMT_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFN6835988.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4359..5450
                     /gene="phnW"
                     /locus_tag="ACHGMT_002264"
                     /pseudo
     CDS             4359..5450
                     /gene="phnW"
                     /locus_tag="ACHGMT_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
     gene            5443..6228
                     /gene="phnX"
                     /locus_tag="ACHGMT_002265"
     CDS             5443..6228
                     /gene="phnX"
                     /locus_tag="ACHGMT_002265"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050194 - phosphonoacetaldehyde hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFN6835989.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(6574..8265)
                     /gene="argS"
                     /locus_tag="ACHGMT_002266"
     CDS             complement(6574..8265)
                     /gene="argS"
                     /locus_tag="ACHGMT_002266"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFN6835990.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8687..9634)
                     /gene="arcC"
                     /locus_tag="ACHGMT_002267"
     CDS             complement(8687..9634)
                     /gene="arcC"
                     /locus_tag="ACHGMT_002267"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFN6835991.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9749..>10689)
                     /gene="argF"
                     /locus_tag="ACHGMT_002268"
     CDS             complement(9749..>10689)
                     /gene="argF"
                     /locus_tag="ACHGMT_002268"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFN6835992.1"
                     /translation="FSEHLKDLKKRGIPHHYLEGKNIALLFEKTSTRTRSAFTTAAID
                     LGAHPEYLGANDIQLGKKESTEDTAKVLGRMFDGIEFRGFSQKMVEELAEFSGVPVWN
                     GLTDEWHPTQMIADFLTIQENFGTVEGITVAYCGDGRNNMANSLLVTGAILGANMRIV
                     APKELQPEEEIVKMAEGFAEKSGAQLMITDDVDKGVDGADVLYSDVWVSMGEEDKFEE
                     RIKLLKPYQINMEMVEKTHNTDRLIFLHCLPAFHDTNTVYGEQMKERFGITEMEVTDE
                     VFRSKYARQFDQAENRMHSIKAIMAATLGNLFIPRV"
BASE COUNT         2940 a         2218 c         1931 g         3600 t
ORIGIN      
        1 tgaaatcatt gtttttttat ttaaaaactg aataattttt tcttttttta atttttaaat
       61 aaaagaaata taaataaaaa cagaggaagc ggaatgatcc gcccctctgt ttttatgatt
      121 ttattaaact tccaataatt ctttttcttt atcagcagcg atcttatcta cttctttaat
      181 gctgttatct gtcaatgttt gtacgtcttt ttctaatccg cgcaaatcat cttcggtaat
      241 atcaccattt ttttgttgtt ttttatattc atcgatcgca tcacgacgga tgttacgaat
      301 cgcgattttt gcattttctg cttctttttt cacatcttta gctaattctt ttctgcgttc
      361 ttctgtcagt tgcgggataa ctaagcgaat gacatttcca tcattggttg gactgattcc
      421 gatatcactt gccataatag ctttttcaac atcttgaaga atgctcttgt caaaaggtgt
      481 gatcattaaa acacgtgctt ctggaatttg gattgatgcc atttggttca atggtgttgg
      541 cgcaccgtag tagttcacag taatacggtc caaaaggcta gcgttcgcac ggcctgcgcg
      601 gatttgccca agttcacgtt gaagattttg tgctgctttt ttcatttttt cttttgcttc
      661 tgtcataatt gcatctgcca tttttatttc ccccttacag ttgttccgat attttcgcct
      721 aagattgcac ggcgaatatt tccgtgttca tttaaattga agacaagcag tggaatatca
      781 ttatccatgc ttagtgaact tgcagttgaa tccatgactt gtaatccttt ggcaataact
      841 tccagatgag tcagttcttc aaattttact gcatttgcat ctactttcgg atcagcagaa
      901 taaacaccgt ctacattgtt tttagccatc aggataacat ctgcgccgat ttctgccgct
      961 cgtaatgctg ctgttgtgtc tgtcgagaag tacgggttgc cggtaccgcc tgcaaagata
     1021 acgatccgtc ctttttctaa atgacgttct gctttacggc gaatatatgg ctctgcgatt
     1081 tgacgcattt caatcgaagt ttgcacgcgt gtcggtacac ccacgttttc caacgtatcc
     1141 tgtaaagcta aggcattcat aactgtagct aacataccca tgtaatcagc ttgagcacgt
     1201 tccatgccca tttgtgctcc gatttgtccg cgccagatat ttccgccacc cacaacgatt
     1261 gccatttcaa cgcccagctc gtgaacttct ttgatttctt ggatgatttc tttgattact
     1321 ggtggtttga ttccaaatcc tgcatcccca gctaaagctt ctccactcaa ttttaagact
     1381 acacgctgat atttcggtgt gaccatgttc attcctccaa tattttctat tgccatttta
     1441 ccatacttag atgatttcga cactctaaga ctggtttttc ttgttttgct taaaaaaagg
     1501 gagcgcacaa accgtgcgtt cccgtctgtt attcaacaga attatttttt cacttggctc
     1561 ataacttctt ctacaaagtt atcttcgcgt ttttcgatac cttcgccaac ttcgaaacgt
     1621 acgaatgttt taacagttgc gccttttgat gcaacgtatt tttcaacagt catatcagga
     1681 tctttaacga atggttggtc caccaaagcg atttctgctt tgaatttctt caagcgtcct
     1741 tctaccattt tttcaacgat attcgctggt ttgccttcgt tcaatgcttg ttctgtaaga
     1801 acagtttttt catgttctaa ttcagcttct ggaatttgtg attcgtttac ataacgagga
     1861 ttgatagctg ctacgtgcat agctacgtct cttgcaactg tttcgtcagt tgttccatct
     1921 agaacagtca atacggcaat acgtccgccc atatgcaagt atccaccaaa tgcagcattg
     1981 tcgtcttttt ctacaacttc aaagcgacgg aagctgattt tttctccgat aacttgagtt
     2041 gcttcgatca agtcatattc aattgtacct ttatctgttt tgattttcat tgcagcttcc
     2101 atgtctgctg gtttgttttc agcaacaagt tctgcaattt cttttactaa atcttggaac
     2161 atttcgtttt tagaaacaaa gtctgtttct gagtttactt caacgattgc tgcagtgtta
     2221 cctttcacag caactgaagc caatccttca gctgcaatgc ggtcattttt cttcgcagcc
     2281 tttgccattc ctttttcacg taaaagatca actgcttttt ccatgtcgcc ttctacttca
     2341 actaatgctt ttttcgcatc catcattcct acgccagtca tttcgcgtag ttcttttacc
     2401 attttagctg taacgtctgc catgtttaac tcctcctgtg tgtttttctt tttaaaaaaa
     2461 gctgcttcaa aggagagtga ggcttgtcgc ccagcctttg aaacagctcc atcattcaaa
     2521 attattctgc tgaagaattg tcgccttcaa caacatctac gatttcttcg attgatgttg
     2581 ctgtgttttc ttctgtgaaa tcttcttcaa ctacttgatc ttcaccttgg tttccttcga
     2641 tgaaagcatc agccattttt gaagtaatca atttaacggc acgaattgca tcgtcgtttg
     2701 aagggattac tacgtcgatt tcatctggat cgcagtttgt atcaaccata gctacgattg
     2761 ggatatttaa tttgtgtgct tcttgaacag caatacgttc tttacgtggg tcaacgatgt
     2821 acattacatc tgggattctt ggcatatcag cgataccgcc taagaatttt tcaagacgtt
     2881 cacgttcttt gttcaaacca gcaacttctt ttttaggtag aacttcaaaa gttccatctt
     2941 cttccattgc attgatttgt ttcaaacggc tgatacgttt ttggatagta tcccagtttg
     3001 tcaatgttcc gcctaaccaa cgatggttaa cgaagtattg tcctgcacga gttgcttctt
     3061 ctttgattgc ttcttgtgct tgtttttttg tacctacgaa taatgctacg ccgccttctt
     3121 cagcaacgct cttcatgtaa tcataagctg catctactaa tttaactgtt ttttgcaagt
     3181 caatgatgta gattccgttt ctttctgtga agatatattt cttcattttt ggattccagc
     3241 gacgagtttg gtgaccaaag tgtacgccgg cttctagtaa ttgtttcata gaaattactg
     3301 ccatgtttgt ttcctccgat ttggttttat ttttcctctt cttatcttct accttgccgg
     3361 caaacttttt caagcaccta ccgacaatcc aataagaatg tggatttgct gatgcataaa
     3421 gcaccctcag ctattataca gacaaaagat ccgtatcgca agagcaaata tacttttctg
     3481 ctcaacattc aatagtttac ctgaaagata aaaagaattc aagaaaaaca gtagatttct
     3541 ctttgtttct ttattatcct ttttccataa aataatttta ggtataaaag acgaatatta
     3601 ttgggcaatc cttctttttt ttgttatgat acatgtgaaa tctttcgtaa aggagaaaga
     3661 aaatgcgcaa agctcaaaga catttgctga ttaaacaagt cattgaagaa ttcccgatcc
     3721 gtacgcaaga agaactatta aacaaacttg ggcattatgg cgttaccgcc acgcaggcaa
     3781 cgatttctcg agatatccgt gatctaaaaa tcgtgaaggc acctgatgaa aatggcgttt
     3841 cccgcttcgt attgtttcgc gaaaaaggaa ctcctgagac taggactgag gaagaggatc
     3901 ggctggttca aatgattgaa gacattgtat tgaaagtcga acgagtccac tttttgacga
     3961 tcgtcaatac cttgccagac aatgctcatt tatttgcatc tgttttagat gagatcaaac
     4021 ctccctctat tgtcagtaca attgctggat tcgatacgat catggtcatc tcggaaaatg
     4081 aggaggacgc aaaacgagta gaagaatttc ttcaccatcc aactgaagta tcactttttt
     4141 aatcagagca cgtattccta atatggaatg cgtgtttttt tattttcaat attttcctta
     4201 ttaaataaac ctttacactc tttacaaaaa tacatcattt tttattcaat tttttcaaca
     4261 ccttgattga atcacaataa tcatcattta tattggatgt taacaagagc agcttcttgc
     4321 tctttgtgta tttcaaaaaa gaaatgaaag gaaatcatat gtataaatta ttaactcctg
     4381 gaccattgac gacttctgaa acagtgaaaa atgctatgct gatcgatcat tgtacttggg
     4441 atcaagaata caaagaaatt acacaatgga taagaaaaaa attattggat cttgctcaag
     4501 tatcggaagg aatatacaca acggtcttaa tgcaaggtag cggctccttt ggggtagaat
     4561 cggtattaag ttctgtgatc aggccagaag atactgtact gatttgttca aacggcgcat
     4621 atggacaacg aatgatcaac atgtgcgaat gtctgaaact gaactatacc gtttatgagg
     4681 tatcgtaaca tcaaatcctc caagctcatg tgattacaca gctgattgaa gagaatccct
     4741 ctatctccgc actagcaatg gtccatagcg agactacttc cggtattttg aataatttgg
     4801 aagagattgc tgctgtcaca caagaaaaag atcttttgtt tatagtggac gcgatgtcaa
     4861 gctttggtgg gataccgatt cgtgtagccg aactagggat tgattttttg atttccagct
     4921 caaacaaatg tattcaaggt gttccaggct tttcttttgt acttgctaat aaagcggaac
     4981 tagaaaagcg aaaaaatgtg agtcgaagtt tgtcacttga tctatatgac cagtaccgcg
     5041 tgatggaaaa cgagaatggc aaatggcgtt tcacttcccc tacccatacg gttttggctt
     5101 ttcaacaagc gctgaaggaa ctagaggcag aaggaggaat cgaaactcga tattcacgtt
     5161 atctcgccaa taatcatgta ctacgacaaa aaatgacaga gatggggttc cagcctttca
     5221 ttcaagaata tcaggggcca ttcatcacct cttttcttta ccctaaggac tggcgatttg
     5281 attttaatca tttttatcag tatttaaagc aaaacggatt tgtgatctat cccgggaaaa
     5341 tcagcgatca tgaatgtttt cgtatcggaa atattggcga gatctatccg gaagatattt
     5401 tggaactgac agcaattatc aaagactata aggagaacgt taatgattaa aactgtaatt
     5461 tttgattggg caggaacaac tgtggacttt ggctgtatgg ctcctgtcca cgcttttaga
     5521 aatgcttttc tagaaaaagg aatccaactg actgacaaag aaatccgcga accgatggga
     5581 aaattaaaat gggatcatat ccaacagctt ctttccctcc cttctgtcaa aaaacaatgg
     5641 atagctattt atggacaact acctcaggaa aaagaggtgg aggaattgta tcaacaattt
     5701 gaacattatc tgtttaaaga tttggtcaag cattctacct tgaaacccga cacattggaa
     5761 acgatcgaac ggttgagaaa cgctgggatc aatattggtt ccacgactgg atttactgct
     5821 cctatgatga caatcgtagc agaaaccgca aaaaaagaag ggtattcacc agacttcatg
     5881 gcaacttctg aagacgtcga agggtatggt cgaccgtttc cttatatgat ccataaaaac
     5941 atgcagtact tcaaaaacaa gtcggttgat gaagtaatca aagtcggaga tacggtctca
     6001 gatatccagg aagggaaaaa tgccggagtg ttgacagtag gtgtaattga aggtagttcg
     6061 ctaatgggga tttcttatga tgagtaccaa aaattagaga tggggaaaaa agatcgttta
     6121 aaacagtctg ttgctcttag attccaacaa gctggcgctg atatttgtgt aaacaattta
     6181 aaagagttaa cagaatacat tttgtacgct gacaagaatc cagaatgact tttatacaaa
     6241 atcagaggtc gtgaaaaagc tgtactgcat caagtaataa gaacagccaa acaaaaatag
     6301 ctcctcatat ttttcgtttg gttaggctta ttaccgcaga tgcaagcttt tgaacaccgt
     6361 ttattcgaaa aaaaaaggga gctgtgacac gacttttgtc acagtcccct tatttacaaa
     6421 caaacagtgt ttaagagaca acctctccac taaacggtgt tcattcagct gactctcggt
     6481 atttgctcaa aaaacgataa aatttgaaaa gctattttct cttttgtttc tctaatactc
     6541 ggatcagaac gctgatttca cattctcttt ttcttacatc ttatctggtg catgaagtcc
     6601 tagcaaacga aggtcttctt tcaataatac agtcactgca taaactagag ctaagcgtgc
     6661 ttctttttgt tcatcatcag ctaaaatctt cgtatgagca tagtatttgt tgaatgcttg
     6721 tgctaatttg attgcatgtt tcgcaatcac tgacggttcg tatttttccc cagcagacaa
     6781 gaccgtttca gggtattttt gaactaattt aaccacttcc cagctggtat cgtcattcaa
     6841 tgcatagtct gctgtttctg aagggacaaa tcctgctttt tccaacaggc tgactgcacg
     6901 agcatgtgta tattgcacat aaggtccagt ttctccttca aatcgaacaa cttcttccag
     6961 gttaaagtca aatgtattca gtcgatcgtt tttcaaatcg tggaaaatga ctgccccaac
     7021 cccaacttgt ttagctacag catctttgtt ttccaaatca gggtttttct cgctgatttg
     7081 ttctttagca gattggattg cttcatttaa tacttcttct aataagacga tctttccttt
     7141 acgagttgat aattttttac caccttgcgt gatcaaacca aatggaatat ggcgcatatc
     7201 gtcagaccag tcaaagccca tttccttcaa gacagctttc aactgtttaa aatgatagct
     7261 ttgttcattc cctactacat atagagattg tttgaagtcg taagtacgtt tacggtacaa
     7321 tgcagcagct aagtcacgtg taatgtatag tgtcgcaccg tctgacttct taatcaacgc
     7381 tggattcagg tcataagcag ataggtcaac gatctctgca cctttatcct cgttcagcaa
     7441 atgtttttct tctaaaagtt tgacgatttc atccatcttg tcattgtaga aagcttcacc
     7501 gttcaacgaa tcgaaacgta cttccagcag atcatagatt ttattgaatt ctttcatcga
     7561 ttcatcacgg aaccattgcc ataattgaat agcctcttca tcgccttctt ccagacgttt
     7621 gaaccaagca cgagcttctt catttaattc tggttctgtt tcagctactt catggaattg
     7681 gacatataaa cgaagcaatt cattgattgg ttctgcttta acagcttctt ctgtacccca
     7741 ttttttgtaa gcgacgatca gttttccaaa ctgtgttccc cagtcaccta aatggttgat
     7801 gcggattggc tgataaccga ttttttccat aatgaaaccg atcgaattac cgataactgt
     7861 cgaacgcaaa tgtcccattg agattggttt ggcaatatta ggagaagaca tgtcgattgg
     7921 tactgttcct tgattcccga tattgctatc cccataatgt tctttttctt tcactactgt
     7981 ttgcaaaact tttttgctga taagttcttt attcatgaaa aagttcaagt aaggtccgac
     8041 aacttcgatt ttttcaaagt tggcagaatc gattttttct gctaaatctg cagcgatttg
     8101 ctgtggcgct tttcggtaaa cttttgctaa tgaaaaggct ggaaatgcaa catctccgtg
     8161 ttcagctgat tttggatttt ctagtaattg ttcgacttgt tccagtgtta aatcatcttt
     8221 gacagcgtca tagacagctt ttgcgacaag atctttatta ttcatcttta ttcctcctaa
     8281 agtgctccct tgattgttaa acaaaaaaaa tctctagcga cggcatactt agtatgccat
     8341 cactagagac gagatttccc gcggtaccac tccaattgtc ctaaaaaagg accagctcca
     8401 tctgatatcg gtagacgacc gtctgtttaa aacagaattc tccaagtgcg cttcgttgta
     8461 tcttcttcat tcggcttcca ccacttccga attcgctagc caagaatgat acaagtactc
     8521 tcttgttcaa caaatcttag tcttattaaa ctttcttgaa ttatatcaga aaaaaaaacg
     8581 aaaggctagt cgtttctgat aaaaaaacaa tccagatggc ctaaaggaga agaaatacca
     8641 cctggatagc tataaatcat ttcgtaaata cgaaagactt aacaaattaa tcagctacga
     8701 taatcgttcc ttcttcagaa gctaaaagat tctcgatatt ttctaaagaa gtgatgattg
     8761 ccttagcgtt tggttttgct tcaacaaatg caatagctgc ctctactttc ggaagcatgc
     8821 ttcctggagc aaattgcttt tcatcaatgt attgtttcat ttctgaaaca gtaacagttt
     8881 ctaatttctt ttgatctggt ttttggtaat tgacatacac attgtctact cctgtcaata
     8941 cgatcagcaa atccgcatcg atgatttcgg ctaatttttc tgaagcaaaa tctttatcga
     9001 ttacggcttc tcgtccttct aatccagtag ctgtttctac aacaggaatc ccaccgccac
     9061 caacagagac tgtgataact ccttgatcga ccagcgtttc gatgacacgt gcttctttaa
     9121 tcgagatggg tttaggggaa gcgactactt ttctccatcc acgtccagca tcttctttga
     9181 atgtcacagt ggagtcagca ttcatttgtt ctttggcttc ttcttccgtg tagaatggac
     9241 cgactggttt acttggattt ttgaatgaag gatcattttc atccacaata acttgagtga
     9301 ctaaagaaac cacatcttta tcgatccctt tttccttcag tacttcgccc atggcatttt
     9361 gtaaccagta accgatagat ccttctgtca ttgcaacaca tgtatctaaa ggcattgccg
     9421 gtgttttctc agaatcagca gcttgctgct gaatcaatag gttaccgact tgcggaccat
     9481 ttccgtggga gatgatcaat tcatctcctt gttcgataaa tttcactaaa tactttgctg
     9541 tttccattaa cgcttcttgt tgggcttttg cacttgcatc cgtagacaag attgcattac
     9601 cccctaaagc gacaactact tttcgtttgg ccatttcatt cgtcctttct tcagttctac
     9661 cgaattttct agataacaaa acgagccagc gttttgtgat tttccctcaa tgctgactcg
     9721 ttttttcgtg agatgaatgt ggttatgctt aaacgcgagg gataaacaag ttacctaatg
     9781 ttgctgccat gatcgctttg attgagtgca tacgattttc tgcttggtca aattgacgag
     9841 catatttgct gcggaagact tcatctgtta cttccatttc agtgatgcca aagcgttctt
     9901 tcatttgttc accgtaaaca gtgtttgtat catggaaagc tggcaagcag tgcaagaaga
     9961 tcaaacgatc tgtgttgtgt gttttttcta ccatttccat attgatttgg taaggtttca
    10021 acaatttgat acgttcttcg aatttatctt cttcacccat tgatacccat acatcagaat
    10081 aaaggacgtc tgctccgtct acacctttat caacatcgtc ggtaatcatc aattgtgcac
    10141 cggatttttc agcaaagcct tctgccattt tgacgatttc ttcttcaggt tgtaattctt
    10201 ttggtgcaac gatacgcata ttcgctccta aaattgcacc tgtgactaat aaagagttag
    10261 ccatattgtt tcgaccatct ccgcagtaag caacagtgat accttctact gttccaaagt
    10321 tttcttggat agttaaaaag tcagcgatca tttgtgttgg atgccattca tcagtcaagc
    10381 cattccaaac tggaacacca gagaattctg ctagttcctc taccattttt tgactgaatc
    10441 cacggaattc gattccatca aacatacgac ctagtacttt agctgtatct tctgtggatt
    10501 cttttttacc taattggata tcatttgcac ctagatattc aggatgtgca cctagatcga
    10561 tcgctgcagt agtaaatgca gaacgagtac gtgtagatgt tttttcaaat aaaagcgcaa
    10621 tattttttcc ttctagatag tgatgtggga taccacgttt tttcaagtct ttcaagtgtt
    10681 ctgaaaaat
//
LOCUS       DAZBSA010000094        10309 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000094 DAZBSA010000000
VERSION     DAZBSA010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.094"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            179..409
                     /locus_tag="ACHGMT_002269"
     CDS             179..409
                     /locus_tag="ACHGMT_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFN6835993.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            506..1807
                     /gene="murA"
                     /locus_tag="ACHGMT_002270"
     CDS             506..1807
                     /gene="murA"
                     /locus_tag="ACHGMT_002270"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFN6835994.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1820..1996
                     /locus_tag="ACHGMT_002271"
     CDS             1820..1996
                     /locus_tag="ACHGMT_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFN6835995.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2131..2427
                     /gene="yidD"
                     /locus_tag="ACHGMT_002272"
     CDS             2131..2427
                     /gene="yidD"
                     /locus_tag="ACHGMT_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFN6835996.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2612..3079
                     /locus_tag="ACHGMT_002273"
     CDS             2612..3079
                     /locus_tag="ACHGMT_002273"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6835997.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3164..4048
                     /locus_tag="ACHGMT_002274"
     CDS             3164..4048
                     /locus_tag="ACHGMT_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFN6835998.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4162..4398
                     /locus_tag="ACHGMT_002275"
     CDS             4162..4398
                     /locus_tag="ACHGMT_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFN6835999.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4486..4566
                     /locus_tag="ACHGMT_002276"
     tRNA            4486..4566
                     /locus_tag="ACHGMT_002276"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4520..4522,aa:Tyr,seq:gta)
     gene            4573..4644
                     /locus_tag="ACHGMT_002277"
     tRNA            4573..4644
                     /locus_tag="ACHGMT_002277"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4605..4607,aa:Gln,seq:ttg)
     gene            4769..4939
                     /locus_tag="ACHGMT_002278"
     CDS             4769..4939
                     /locus_tag="ACHGMT_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836000.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVRVKSKRP
                     TNHTKKWMAKNR"
     gene            5159..5866
                     /locus_tag="ACHGMT_002279"
     CDS             5159..5866
                     /locus_tag="ACHGMT_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN6836001.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5925..6569)
                     /locus_tag="ACHGMT_002280"
     CDS             complement(5925..6569)
                     /locus_tag="ACHGMT_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFN6836002.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6695..6982
                     /locus_tag="ACHGMT_002281"
     CDS             6695..6982
                     /locus_tag="ACHGMT_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836003.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7090..7410
                     /locus_tag="ACHGMT_002282"
     CDS             7090..7410
                     /locus_tag="ACHGMT_002282"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN6836004.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7491..8804
                     /locus_tag="ACHGMT_002283"
     CDS             7491..8804
                     /locus_tag="ACHGMT_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFN6836005.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            8801..9520
                     /locus_tag="ACHGMT_002284"
     CDS             8801..9520
                     /locus_tag="ACHGMT_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFN6836006.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9648..10199
                     /gene="raiA"
                     /locus_tag="ACHGMT_002285"
     CDS             9648..10199
                     /gene="raiA"
                     /locus_tag="ACHGMT_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFN6836007.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT         3431 a         1704 c         2201 g         2973 t
ORIGIN      
        1 agggactgcg acaagacttt tgtctcagtc cctttttttg catattttta gcatttattt
       61 cctaaaaaat gtaaggggat tcctatattt ttaagtatac ttgtttttcc ttttatacat
      121 tttatggtat tatcatttta ttagttatta taaaacaatc gttagaaagg acgtcattat
      181 gcaagtatac ggaattgatg cggtgcttcg aatcgtgagt catttggcgt ttatctactt
      241 agccttctgg tctttacgtt cgttacgcat tgaaactttt ttcagaccaa tgcataatac
      301 gcaaattaga atggcaatcg tacttttttc aattttttta gggtacactg caagtaattt
      361 tttcttagaa gttatcgctt tatgccgaaa tcttttcgtt agcctgtaaa ttttaaaaaa
      421 aggtactatt ttaaaagcaa ggatgttata atagaaaaga ttttgagtaa gtgtatgctc
      481 attggaattt tttggaggga acaacatgga agagatcatc gtaagaggtg gcaatcaatt
      541 aaatggaaca gtaagaattg aaggagctaa aaatgctgtt ttgcctattt tagctgctag
      601 tttgttagca gaagaaggta ttacgacatt agacaacgtt ccgattcttt ctgatgtctt
      661 cactatgaat caagtgattc gtcacttaaa cgtagatgta gattttgatg aacaaaagaa
      721 tcaagtaaca atcgacgctt caagacaatt agaaatcgaa gcgccttatg agtatgttag
      781 tcaaatgcgt gcttcaatcg ttgtgatggg tccgctactt gccagaaatg gacatgcaaa
      841 agtagctatg cctggtggat gtgcgattgg taaacgacca atcgatttgc atttaaaagg
      901 atttcaagct ttaggagcaa aaattattca aaaaaacggc tatatcgaag caattgcgga
      961 tgaattgatc ggtaacacga tttatttaga tttccctagt gtaggtgcta ctcaaaatat
     1021 catgatggct gccgtaaaag caaaaggaac aaccatcatt gaaaacgtag ctcgcgaacc
     1081 agaaatcgtt gatttagcaa acattttgaa taaaatgggc gctcaagtct atggagctgg
     1141 tactgaaaca atgcgtattg aaggcgtgga tcatttacat gcagtcaacc actcaatcgt
     1201 acaagatcgt atcgaagcag gtacctttat ggttgctgct gcaatgacac aaggcaatgt
     1261 gctgattgca gatgctatct ctgaacataa ccgcccattg atttctaaat tgatcgaaat
     1321 gggtgcagaa atcattgaag aagaaggcgg agtccgtgtc atcggaccaa aacatatctt
     1381 acctacagat gtaaaaacga tgcctcatcc tggtttccct acagatatgc aagctcaaat
     1441 gacagctatc caattagttg cagaaggtac aagtgtagtg actgaaacag tgtttgaaaa
     1501 tcgtttccaa catttagaag agatgcgccg tatgaacgct catgtcaaaa tcgacggaaa
     1561 tgtagcaatc atggatggaa accatgaatt gcaaggtgca gaagtatatg caacagactt
     1621 gcgggcagca gcagcattag ttttagctgg cttgaaagca aatggaatca cacgtgtgcg
     1681 taacttgaat tatctagacc gtggatatta taatttccat atcaaactac agcaattagg
     1741 tgcagacgtt gaacgtgtag acatggatca aacttctgct gaaaaaacag cacaaacgat
     1801 tgcttaagga agtggctgaa tgagtaacca acgatatatc ttattaactt tgataaagat
     1861 tttaatggtg atcttattgc ttatccttct ttttgtggcc ggaacgatga tcggatatgg
     1921 tgtcatcggt ggcggcaatc catttaaagt atttcagcca agtttgtgga ttcatattcg
     1981 agactttttt cattgataaa taagaatcac agtggttcta gtcgatatga agtgagccat
     2041 gaagcagaac atgataaaag aaaacagaag ggaacgttga cgttagtcgc gatccctttt
     2101 tttagtaaaa gaaaagaagg aatctaattg atgaagaaga tattgattta tttagttaga
     2161 ggatatcagc gctttatttc gcctgcattt ccacctagct gccgttatta cccaacctgt
     2221 tccaattata tgataaaagc aattcaaacc catggcgcgc taaaagggac ggtaatgggg
     2281 actgcacgga tcttacgctg tcatcctttt gtcaaaggcg gtatcgatta tgtaccattg
     2341 acattccgat taacgcgaaa taagaaagat aaaaaagaac cagattataa aaaggttgtg
     2401 aaaaatacgg gtagcactga gcgataaaga aacaatttgg aaaatagctt cttatatttt
     2461 gcaaaattgt gagttattgc cgaagtgttg gattttgaac accgtcaaaa aaaaggttgt
     2521 gaaaaatacg gctagcactg agcgataaag aaaaagagga aatttattgt caaagtaaca
     2581 actccgcaac tacttatatg ggggacaaaa aatgatcgaa gaactaaaca agaaagattt
     2641 accatggtcc ttgttattag atgcagatcc tgataaagaa aaagttcaag tatatgtttc
     2701 cagagggagt ggctggatat ggaaagaaaa agaaaaaaca ataggtgtac tgatctatgt
     2761 ggcaagggaa aaagaatacg aaatcgtcaa tgtagcagta accccaacgt atcaaggaaa
     2821 ggggattggc ggaaaattac ttgagacagc ttttcagaag ctttcacaac tcgcttccag
     2881 tcaaactcag atcatcatac gaaccggcag tactagcagt gcggcgttac atctctatca
     2941 aaaaatgggc tatgtggaga ttggtcgagt caaagactat tttatccatc attactctga
     3001 gcccattttt gaaaatggag atcaattaag ggatcaagtg attttagcaa ttcacttacc
     3061 taaaaatagc tttaactaat aaaatttcaa ttcaagaaaa tgctttcaaa tgctaaatag
     3121 atacattaca atagctttag caaacaaaaa ggagaatatt tttatgggaa tattagtttt
     3181 tgattttggt ggatcggctg tcaaatatgg ttgttgggat ggcaaagaga ttaaaggtaa
     3241 gggacaattt gcaacgcctg aatcttggga agaaatgaaa gcgcaattat ttcaagttta
     3301 taaaaaaata ctcgtttctt ttgaagtcga aggaattgga atcagctctc ctggagtagt
     3361 gaataatgag aaacaggtga ttgagggaat cagtgcgatt ccttatattc atggatttaa
     3421 tattttccga gatttggaaa ctctgtttca acttccagta acgattgaaa atgacgccaa
     3481 ctgtgccgga atggctgaat tttatgaagg tgctgcccgc gattatcaag atgttgcttt
     3541 tgttgtggca ggaactggga ttggcggggc gttgttcacg aaaggacaaa tcaataaagg
     3601 ggcacatctt tacggtggag aatttgggtt gatgttctta aatggcgata agacattcag
     3661 taaacttggt acagcggtac agatggcttg gcgttattgt gaacgcaaag gtctcgataa
     3721 aaatgcttat accggaaaag atgtctttga attagcagaa actggagatg caatagcaaa
     3781 agaagaagtt gagagttttt atacgtatct tacaaaggga ttattcagta ttcaattttc
     3841 gtttgatcca gaagtgatcg tattaggcgg cggggtttct gcaaaagaag ggctcattga
     3901 tgagatcaaa agccgaatga agaagctgac agaacaattc gatctgcatg atttcgaacc
     3961 gcagatttta ttatgtgaat acagaaacga tgctaatctt gtaggtgcag cagcaaacta
     4021 tctcgctgta cgtcaagggg agaattaaga gctttagaga aggtgcagct tgcataaaag
     4081 aagtgaatga aatgaatagg agatttttac ctaaggaatg ttttatttgc tgcagtatga
     4141 taaaatatct gagaggtgaa aatgatgtca aaaaaaaata aagatattga agtgcgtata
     4201 gaagaagtga aaaaagatat caaaggtaag aattatgaag tcactcagtt atttataggg
     4261 aaaaaaatga ttggtgagat cttagcctat ggaccaaaag aatatgaaat tttctttggt
     4321 gaagaagatt ttggaaaaga aaaaagtctt gaaaatgcga tagaaacagt gattcgacac
     4381 tggaatctgc acgaataaaa aaatatcaaa aaaactttca ataaagggtt gcaaaaatct
     4441 ggaaaattag gtattataac taagtcaggt tgatacaaac ctttcggagg agtagcgaag
     4501 tggctaaacg cgacggactg taaatccgtt ccttagggtt cagtggttcg aatccactct
     4561 cctccatttc attggggtat agccaagcgg taaggcaagg gactttgact ccctcatgcg
     4621 ttggttcgaa tccagctacc ccagtatacc acaatcgtaa gctctcgtta gttcgagagc
     4681 tctttttttt gctttgctac cttgttcctt gaagattctg ctgatttaag atagagttaa
     4741 gttaactaca agaattggag ggatccacat gaaatggaaa gaaaaagtag acgaagcagc
     4801 tgaaaagtta tatgatttgg tcaaatcaga aaaatacaat attgaagtga atattccgaa
     4861 aaaaggcgga aaagcggtcc gggtaaaatc aaaacgccca acgaatcata caaaaaaatg
     4921 gatggctaaa aaccgctaat aataacaaaa gatccataag tcggcatttt catcgatttt
     4981 agggtctttt gttattttct aggttggaca tatctaaaaa attgtttctg acaataggag
     5041 taggaagaac gatgtgtgtc taaatcctct gttgatggat agaaaccttt atctttttca
     5101 gttgtgaatc aggtatatac cagttataat agaagaaacg aaaggacggg tgtacgttat
     5161 gtcgaatcaa ttacctgtat atatacagat tcatgaccaa ttaaaaaatg aaatcgaaca
     5221 aggtatatgg aaagtcggag accgtcttcc ttcagaaaga gaattagcag tgaaattcaa
     5281 tgtcagccgt atgacgttga gacaagcaat tcaaaacttg gcagatgaag gaatcttaga
     5341 aagaaagatc ggttcgggta catatgttgc acgagaaaaa gtacaagaaa aaatgtctgg
     5401 tgcaacaagt tttacagaaa ttatggaatc acaaaatcga gtaccgtcta gtcggacaat
     5461 ctcttatttc ttgacttcgc caagctcaag tgaaatggaa aaactaaact tgagtaaaga
     5521 tgacacgatc ttgcgaatgg aacgtattcg tttcgcagat gatgtcccta tttgttttga
     5581 agttgccagt gtgccacaaa agctgatcga tggctacagc aaagcagaga ttactgaatc
     5641 gttgtatcgc acgctagaag aaaaaggcgg gaaaaagatc ggtccagcga atcaaacgat
     5701 tactgctgtg ttggcttctg agaaaattgc tgactattta gaggtaaaac gaggagatgc
     5761 agttcttcgt ttgcgccaga tttcttattt tgaagacggt acgccgtttg agtatgtgcg
     5821 tacgcaatac gtaggaaatc gttttgagtt ttatttggaa aaataaaaaa caggtgatcc
     5881 ctgattccgt attagaaata aaagagaatc agaatcacct gtttttatat atgatcagtt
     5941 accagttgct cgtgataaga aacaggaccc tcttcaaatt ggacttgtcc gttcagcagc
     6001 tcaatgatgg attctttaaa gtggtcagca cttttttcat ctacagcaca gatgacgcta
     6061 acttgatcag tatattggat ttcttttata aaaaaatgtt ggagttcaag ataattctgt
     6121 aattttccta gttgcggata cgtgatcatc acagagatct cctgttgcaa ggtaccttct
     6181 acgattccaa tgtcagacaa tgcttgagag accgcatgag tgtatgctcg tattaatccg
     6241 cctgtgccga gtttcgttcc tccaaaatat cgagtgacta ctgcagctac attgatcagt
     6301 tgattttttt tgaggacttc caacatcggt atgcccgccg ttccacttgg ttctccatca
     6361 tcgctgcttc gctggatttc atttttttct ccaatgatga aggcgctgca gttatggttc
     6421 gctttccaat gttctttttt tatttcttga atgaaacttt ttgcttcgtc ctctgattct
     6481 attcgtctaa gagaacagat aaagcgtgat tttttgatct cgatctcact ttgtccattt
     6541 tcttttattg tacgatagcg ctcgatcatc ttgtcacctc ttttttcatt ttctttattc
     6601 agtataaata aagttctagg ttgtttcaac actgtaacgg ttgcgcaatt gctgtagtat
     6661 gcgataataa agacaaaggc aggtggatct ggggatgcag gaacgaatga aaaagtacga
     6721 tgcaattacg cattatttga agaacaatgg cggctctcaa gtaacgttga cctttaccca
     6781 atttgacgaa ctgctgtttc ccagtaacgg acttcccaaa acagcaaggg aaagtacaga
     6841 ttggtgggca aatgattata aacaccctga aaaaggtgcc tatggctgga taaatgcagg
     6901 ctatgaagta gttgtcatca atttggacaa agaatatgtc gtgttcaata aattagtgaa
     6961 atctagttgg ctatttgatt aaataaaccg cgtttagaaa acgttgacaa atttctttct
     7021 ttttatataa tagagtaaca gacgcgaaga aaacgtttgc gtagtttgtt ttaaaaagga
     7081 gagaaaagta tggctaaaaa aacaatcatg cttgtatgtt cggctggtat gagcacaagt
     7141 ttactagtaa caaaaatgca aaaagctgct gaagcaaaag gaatcgactc tgatattttc
     7201 gctgtgtccg cttctgacgc agataacaat cttgcaaata aagacgtgga tgtgttgtta
     7261 ttaggcccac aagttcgctt tatgaaagct gattttgaga aacgtctgga accaaaaggc
     7321 attcctttag atgtcatcaa tatggctgat tatggtatga tgaatggcga aaaagtgtta
     7381 gatcaagcaa tcacgctcat aggtgaataa atagaacagt ataactatcg aacaagcgat
     7441 ccaaaaaata gatcgcttgt tttttcgtat atttttttag ggcgaataat atgaatcatt
     7501 tttggggtag aaggcttctg acgagagata ttaaagcaat agactgtgag gaggtaaatt
     7561 taccaaatta tgagaagatc gcagcagtgg aacgtttgag caactggatt ctttgtcatc
     7621 gctgtggaat gagaacgtcc gtcattgatg gacagcttgc agattacggt tatttttgta
     7681 cacattgtct atatttaggg cgatgcgaca ctcaacaaga tttatatttg tttgatcagc
     7741 ctgaagcaaa acctcgagaa gtcgtctttt tttggacagg acagcttact gaaaaacaaa
     7801 cggaaatctc aaagagaata ctttgccatt cagaaaaaag acatcatctt atttgggctg
     7861 taacaggtgc agggaaaaca gagatgcttt atcctatttt agtggaaact ttaaaagctg
     7921 gaggaagagt agcgatttgt acaccgcgag tcgatgtttg taatgaattg tttttacgct
     7981 atcgttatgt ttttccagaa gaaaaaatca cgttgctcca tgggaataca gcaacggctt
     8041 atacctttac ttcttttgtt atctgtacga ttcatcagct ataccgtttt tatcggtcct
     8101 ttgatttaat agtgatcgat gagatcgatg cgtttcctta taacggggat gcaggattag
     8161 ctcatgctgt gcaaacagcg attaaaccag acggacgatt catctactta agtgccacac
     8221 ctgataaaaa gttactaaag gagatcaagc agacgtttga catccacaaa cttgctttac
     8281 gctttcatcg gcgtttgctt cctgaacccg tcttggtttt catgaataat tggcgacaaa
     8341 aatgcaagaa caagaagaag ataaaatctt tggtgcggat cattcatcaa ttaattaaag
     8401 aaaaccatat ccttttattt tgtccaagta tcacgatgat gcatcgtttg aaagccacac
     8461 ttcaagaggc tcttccgaga tatgagatca cagatgtttc ttcaagagac aatcagcggg
     8521 cggaaaaagt ccagaaaatg cgagaagcaa aatatgacat actgttaacc acgatgatcc
     8581 ttgaacgtgg agtcacattt gaaagaatat ccgttatcgt tttaggagcg gatcatccgg
     8641 tttttactaa atcttctttg gtacaaatcg caggaagagc cgatcgtaaa gggccttata
     8701 cgaacagtca agtatatttt ttctatgaag aaaaaactgc agctattcga aaagcttgtc
     8761 aggaaatcaa agaaatgaat gaagaaggga agaagatact gtgaggtgca gttgctgcag
     8821 taaagatatc gtccaaaatt taacattcag cgagattatt ggtgtttcga aatacagatt
     8881 acctcagact tgttcagttt gtcgcgcaca atttcattta ttggaaacaa gaggctgttc
     8941 tggctgtcaa aaaccaatcc atttcaatct agagagtacg aaaaagaaag taaattctat
     9001 ccccgtttac tgcagtgact gtcaagaatg gaagaagaaa tatccgacct atgattttca
     9061 gcaccgtgcc ttttttgtgt acgatgaaaa tatgaaggcc tggctcaatc aatacaagtt
     9121 tatgggagat atccgactag caggcacctt tgcatataaa tggacgcaga taaaaaaaga
     9181 atacccgaca tacatctatt gtccaatacc tttatctgaa aaaaggcgaa cagaaagagg
     9241 attcaatcaa gtaagcgaga tgttgaaagc agcaagaatt ccttttcaga tgctactcaa
     9301 aagagagaag catctgctgg ctcaagcaaa aaacacgcga aaggaaagat tggagatgcc
     9361 tcaacctttt gaacttgcag tcaccttttc taagatacaa aataaaaaaa tattgattat
     9421 tgatgatgtc tatacaacag gacagactct tttccacgca gcagattgtt tgcagatcgc
     9481 acagccgaaa atcatccgaa ctatttcttt ggcgagatga attagatgtg gaaatttgat
     9541 agcgatttaa tttgcaaata acctagagtt cgatataata gaattaagaa gagggaaaga
     9601 gtggtccttc tccttcttga ttgtggtaga cgaaaggggt aatttttatg tttagatata
     9661 atgtacgcgg cgaaaatatc gaagtaactg aagcgattcg cgactacgtt gagaaaaaag
     9721 taggcaaact agaacgctat ttcagtgacg cacccgatgc aacggcgcat gtgaacttaa
     9781 aagtttatac agaaaaaaca gcaaaagtgg aagtaacgat cccattacct tatcttgtat
     9841 tacgtgccga agaaacttct ccagatttat acgcaagtat cgatttggtt gttgacaaat
     9901 tggaacgcca aatccgcaaa ttcaaaacaa aaattaatcg taaatcccgt gaaacgggca
     9961 tgaatactgc aaatgctgct gctttattca atgaagagga cgaaaacgaa aatgattctg
    10021 aattagatat cgtacgtacg aaacgccttt cattgaaacc aatggatagt gaagaagcag
    10081 tcttgcaaat gaacatgctg ggacataact tctttatttt tgaagatgca gaaacaaatg
    10141 gtacaagtat cgtttaccgc cgtaaagatg gaaaatacgg actgattgaa acagactgat
    10201 cagccttact taaataacaa cgaaaacttg cagcagctac tgacagctgc cgcaagtttt
    10261 tttgagtttc ctcctctttt agatgaaata tgaataaatt atgaagttc
//
LOCUS       DAZBSA010000095        10206 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000095 DAZBSA010000000
VERSION     DAZBSA010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.095"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..465
                     /locus_tag="ACHGMT_002286"
     CDS             <1..465
                     /locus_tag="ACHGMT_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFN6836008.1"
                     /translation="WKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQAAYAF
                     LKRLVKQFDEPKVVVTDKAPSIASAFKKLKEYGFYQGTEHRTIKYLNNLIEQDHRPVK
                     RRNKFYRSLRTASPTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPD"
     gene            complement(1080..2120)
                     /locus_tag="ACHGMT_002287"
     CDS             complement(1080..2120)
                     /locus_tag="ACHGMT_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFN6836009.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(2469..2795)
                     /locus_tag="ACHGMT_002288"
     CDS             complement(2469..2795)
                     /locus_tag="ACHGMT_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836010.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2782..3570)
                     /locus_tag="ACHGMT_002289"
     CDS             complement(2782..3570)
                     /locus_tag="ACHGMT_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN6836011.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3938..4228)
                     /locus_tag="ACHGMT_002290"
     CDS             complement(3938..4228)
                     /locus_tag="ACHGMT_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFN6836012.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(4364..4543)
                     /locus_tag="ACHGMT_002291"
     CDS             complement(4364..4543)
                     /locus_tag="ACHGMT_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836013.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4540..4890)
                     /locus_tag="ACHGMT_002292"
     CDS             complement(4540..4890)
                     /locus_tag="ACHGMT_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836014.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4883..6208)
                     /locus_tag="ACHGMT_002293"
     CDS             complement(4883..6208)
                     /locus_tag="ACHGMT_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFN6836015.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6658..7272
                     /locus_tag="ACHGMT_002294"
     CDS             6658..7272
                     /locus_tag="ACHGMT_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN6836016.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(7437..7679)
                     /locus_tag="ACHGMT_002295"
                     /pseudo
     CDS             complement(7437..7679)
                     /locus_tag="ACHGMT_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7883..9202)
                     /locus_tag="ACHGMT_002296"
     CDS             complement(7883..9202)
                     /locus_tag="ACHGMT_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFN6836017.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(9336..9944)
                     /locus_tag="ACHGMT_002297"
     CDS             complement(9336..9944)
                     /locus_tag="ACHGMT_002297"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836018.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSLNNSYF"
BASE COUNT         3157 a         1828 c         1592 g         3629 t
ORIGIN      
        1 tggaaaatgg atgaaacgta catcaaaatt aaaggaaaat ggcattattt gtatcgagcc
       61 atcgatgcag atggtttaac cttggatatt tggttacgta aaaaacggga cacacaagca
      121 gcctatgctt ttcttaagcg gttagtgaag cagtttgatg aaccgaaggt tgtagtcaca
      181 gataaagcac catcaattgc tagtgccttt aagaaactaa aagaatacgg attttatcaa
      241 gggacagaac atcgtaccat taaatacctg aataatttga ttgaacaaga ccatcgtcca
      301 gtaaagagac gcaataaatt ctatcgaagt ttacgcactg cctcacccac gattaaaggc
      361 atggaagcca tccgaggatt atataagaaa acccgaaaag aaggcactct cttcgggttt
      421 tcggtctgta ctgaaatcaa ggtattattg ggaatcccag attaaatcat agataccgta
      481 agggatttta ttctttattt aaaactttgc aacagaaccg tccgttgcgg gtaataccgt
      541 aacactgaag ggcacgcatt aaggcgtcat cttaatgctt tcctgtagga cactgacgga
      601 gattaccagt ctcccgagcc ttacaggggc ctttcttttt ttgtattttc aattatcaca
      661 atttatcgtc caaaagtgca gaacgaaaat caattcatca caagcatatc agacaattaa
      721 atttaaaaca actaaaaata tacaaaaaag tgtaattgaa tattagcagc actgcctgaa
      781 aagaagtaat atcaggcttt tcagcttggg gaaataagtg aattacctct cttcatgaat
      841 tattataact caaaaaagca catatgcaaa cactagttcc ctattttttt gaatgaaaaa
      901 taactaaaat atctaacgat tatgacttta aaacctacgt ttgcgattgt tttcataaac
      961 ttttaatcta aaaactccgg aataatagct atttatttgt aacatttgta ataaaattaa
     1021 aatcataaaa aagttctaat ttaaaattga tcatttttat agtgatcgct ttttatcaac
     1081 tactctagcc aattttccgt actaactgtt ggtaattcgt taaatgtttc tctttgttcg
     1141 tttgcaagct ttgccgcaat agctttttcg acaaaccaat tttttacata aacgaaaatt
     1201 agattctgca aattgtttac tttttcagtt ttttgttttt gataagcttt cagcaaagtc
     1261 atatataatc ctttgtcagc atcaattcca taagattcca gttcttcata aacaacaact
     1321 gtttgctcta attcttcagc ttttttcttt gcattatgaa tagtttggac ggttttttga
     1381 gcgtcttcgt aagtactaga aaatgttttt atcaagttta atacattttc tggtacaaat
     1441 gtatttatac tgtcctcttt catgatgtcg acgaagttat ctagtaaaac ttgattttgt
     1501 aactgatcag tttcagtgtc tattatgtgt ctattagtgt ctatagaatt agaatagaat
     1561 agattgtctg cgattttcgc aattacggtt gagtccttga tatgactgga ttttgccgta
     1621 tttgcgattt tcgcaattac ggtggtatca ttgatttctc gcggttttgc cgtatttgcg
     1681 attttcgcaa ttacggtggt atcagtattg tcttcctctt ccatgatttt aaagacgtct
     1741 tgattcgtta cttctggctt gcctaagtac aataaattag gcatggattc aatcttaccg
     1801 tcacgtttat ttactcttcc tctcttctgt ttcagaagac ctgctttttc taattccttt
     1861 ttgattttag atactttacc ttcgcggcat tgtaacaatt tcattaattc ttcaactgtg
     1921 aatatgaaaa atatattatc atcagaatct acccagttat ttttgactga ataatcaaat
     1981 cgatctttaa gtagcatata agcaattttt gcatcatttg atagatccat gtatttttcg
     2041 ttagtaaaaa ataccttagg catttgataa tatttttcac gataaatttc ttttgatttg
     2101 taataattaa aatcattcat agccaatcct ctttctgtag gatagcgttt tgttcaaaac
     2161 tatgttacaa tgaattaaac aaatatatct atccactata tatttttcta ctgctgataa
     2221 gtcgccaaac tttgttaaag cagtagtctt tttttatctt tttttagttt accttaaaaa
     2281 ataaattaaa gcaaaccgct ctatttcaag gtttaagctc atagtaacta tatcacgata
     2341 ttacaataat attatatcac gatatcgtaa tattacaata tgaagataag cgacttatca
     2401 gctttgatac tactgatttt tataaaaaaa atatcacgat attatgatat cgtgatattg
     2461 aatgtaaatt aaattttatt ttgtttcttt gctagtttat atccttcaat caagtattct
     2521 tcaggtatgg actttaatag cgcatcttct ataacgtctg gtttactttt gtctgattca
     2581 gcaatagtcg tccaatattt gagtgctgac attagaattg gatttatacg aatgctatct
     2641 gctgttctta cactacttcc ttgatctttt atgactttac ttatattttt cttggttaat
     2701 tcgatttctt ctacttcttt tgatttaaaa ttctttttat ttttatttcc tagtaatcta
     2761 cttactgcag cttgactgcg attatcttct gccatcaaaa tcaccaactt cttttaaata
     2821 ttcttctgta aatgctttat agtcatcttc agctgttgag ggtttattaa aattgattag
     2881 atctgatcca gcagcttgcg cttcgtcaac ggttattgtc ctacgaatat aagttttgaa
     2941 aatttttgca ccaatgactc tagaaatttc ggttgctgca gacgtcattt ctttagaaat
     3001 gttataacgc ttattatact tagtaaatag aactcctcgt acttcaagct tgtcgttcgt
     3061 atattctttt accgtttcaa tagtttcact taactttatt agtcctttta atgagaaata
     3121 cgcagcttca actggaatga taatttcatc agaaacagta aatgcgttga ctgtaagcat
     3181 tcctaaactt ggtggacaat caattataat ataatcatag ttgtccttga ttggttgaag
     3241 tactctttta agcctgtgtt cacttcctac ttgagtaaat tccctctctg cagaagaaag
     3301 ggctatgtcg gctggcaaaa catcaattcc tttatagtcc agaatagcat ctgtaatgtc
     3361 agcctcccct tttaatactt cataaattgt aggttggttt gttccatcta tatcgaaacc
     3421 tacattatct gtagcatttc cctgaggatc aatgtccact aaaagtacat ttttaccgta
     3481 aacaaatttc aaacatgctg ctaaattaac gctagtcgta gttttagaaa ctccaccttt
     3541 ttgattggct actgacaata ttcttgccat taaaatatcc cccattcatc ttatattata
     3601 atataattat atcataatat tgtgatattg caatatcgta tatgcattat attgtaatat
     3661 tgtgtgatta ttttgtttta aagatatata tcggttcgga tataaagatt attcatatgt
     3721 tattcgaaat taaaataatc actttttatt actttaaaat tttttattaa tgataagaaa
     3781 tatgttttgc gagcagcaac tagaataatt taaacattac taaactgtct atattgttac
     3841 taataattcc cttcttatag tgcattttct tagaaaaaaa caatattacg atatcatgat
     3901 attaagatat cgtaatatta tacaattgtg attgatatta tatatgtttt tttatttttt
     3961 tatcatgttt aatcttgaag gaatcatatt ctttttctaa agacaggagc ttttcataaa
     4021 catcagacat gtttaaacta gaggccaggc ttaaatcata aacgaatgaa gcaggtaatg
     4081 attttattct acgttgtcgg gttatccagc ttgcaaccgt tccttgtggg taggcatata
     4141 actcacaaaa ttgttctaca gtgattccta aattcttgat aatgtaaaca ttgatcgggt
     4201 gcgagtaaac aaatgtattg atagacatgg tgcgtaagcc tactttcttt tattattgcg
     4261 tttgcaataa tagtttaaca cacgcattat tctagtatct actcctattt gtaaaaacaa
     4321 aaaaggattc atagagataa ctctatagaa tccgtaaaat accttatata tataacggga
     4381 agatataaaa cctaaaatta tgataacctg ctgcagcttc tacatagaaa gagaagcgct
     4441 caacaccaac ctcatttatc aaacgttggc cataacccca aatctcttct gatagctgat
     4501 caaaggttat atactttagc tcatattgct ggataatgtt cattctaatc gactccattt
     4561 attatcctgg taaaattcga tatgacgtat ttcgtcgtag tgaattttgt cttttccaac
     4621 gtagatcccc agctcatcac tacctaagat cttgccaatg atatcttctg gatagtatcc
     4681 ttcagcgttt cgttcttctt tttgaataga tactgatcta ttttttatga ctgcagcgtt
     4741 caatacttcc gcaatctctt cagtcgtcat ttgttctttt gctaaatttt gactggttcg
     4801 ttgttcattg tcggcatcaa tttcttcagt atgatcagat aaataaaacc ctgcccactt
     4861 taaacgtcca cgatcattgt aatcaatcat ttttaattcc cccatttccg cccgcatgac
     4921 caccaacaag tgtgcttcta gtaatgcttc tagcgccttc taacattgaa gaagcatgta
     4981 caatcgacgt aaaaccatat ttttgccgaa tcttgtcaat aattttatct aaattttctt
     5041 cgtcaatttg cttttgtgga tcactaaata aatccagctg aagcgaatcg gtgtacacaa
     5101 gcttgctata ggttattcca acatgcctta cttcttgtcc ttcataatac ttatcaaaca
     5161 agaaaagaca ataattctgc aattcctttg tattatctgt gatagggatc ttcatctggt
     5221 gtgaaaaccc tggtctcgtt tctaagatcg acgtgccaat gtgtaaatgg acacaacctg
     5281 ttttacagtt atgctgccta atgcgtatcg ctacttgttc cgccatttct tttaccacca
     5341 gctcgatttc atttcttcta gtataatctc gtggtaacac ttgtgaattt ccatatgatt
     5401 tttcctttgt cggttctggt gggattgcta tatctgttcg atcgatccca ttagcatgga
     5461 agtacagctg taagccaatc acacctagtc tattcttaat cgtataagga ttccagttag
     5521 caagatctct aatgctcata atccccatct gattgagtct ttttttcatt cgagagccaa
     5581 ttccccaaaa gtccgtcatt tcaggaatat tccatacttt ttcaggaacg ttttcgtacg
     5641 tccattccgc aacaaatcct tcattatgct ttgcttcgtt gtctaaggct agtttagcca
     5701 aaaggggatt atctccgaca cctactgtag caatcaatcc tagctcttct ttaatattct
     5761 cctggatcat ttgagccagc ttctttctcc gttgacttcg tgttccttca gtcgtaaaaa
     5821 gattcagtga tcgggtcact tttaagattg attcatcgat cgagtaaatc agtagatctt
     5881 catcagccac atatcttctg aaaatattat ttacctgcat atttcgcttg atatataggt
     5941 tcatacgtgg tggaacaacg tgtagtgttt taggaaatgg ttgtggtaag tcacgtggtc
     6001 tactcacatt tgtaatacca taccgctttt ttgcttcagg agaagaagct aatatcaatc
     6061 ctgaaccagt attatcagct cgactcataa caacaagttc tgttgttaat ggatctaaat
     6121 ttctttctat acattcgaca cttgcgtaaa agcttttcat atcgattaaa aagtaatcat
     6181 ttactggctc ttttgaataa tccatcataa ataacactcc attctttcac attatacaaa
     6241 catatgttcg atattgtaaa gaagaaatag ctaaatacat aaaaaaagaa gcataggtat
     6301 gctatcaccc tgcttcttct tttgatttgt atgatcaatt tattctatca ctttactaat
     6361 taatttgaaa atctaataaa aactctcact ctaaaagagt gagagactgc aaattagagt
     6421 acggactttt ttggagttcc gttctttaat attaacaaat gaataaaatc cttacaacat
     6481 ttgagaataa gttatcagta gacacttaca aaaaattctc atactatttc ctccatcatt
     6541 agtttttgtc tatataaggt ttaccctaaa tagacactgg tataacattg aaaaatatgg
     6601 cttcctcgtg tatgattagg gtgtatttta aatagttgca gaaagtaggt aaaagttttg
     6661 agagttgcat acatacgtgt ctcatcgatc gatcaaaatg aacaacgcca gattgaagaa
     6721 atgaaaaaat tcggtgcaga gagaattttc atcgagaaac aatctggcgc aacgattact
     6781 catcgacctg ttttccaaga agcattagat tacgtccgag atcaagatat ttttattgtg
     6841 gaagctattg atcgacttgg aagaaattat gacgagatca tcgcttcagt caattacttg
     6901 aagaagaaaa atgtgaagct gatcattact agtttaccga tcatggctga agcaattggc
     6961 aatcctctcc tggataaatt cattaaggat ctgatcatcc aaattttggc catgatcgct
     7021 gaacaagaac ggactgaatc gaaacgtcgc caggcacaag gaattaagat cgcaaaagca
     7081 aacggcgtct acaaagggcg gcccaaatta tacagtgcag acgcaaaaga tccccaacgt
     7141 cgccttgttt acaaaagcat cgttgaagat cttaaaaatg gtgttgcgat tgctaagatt
     7201 gctaaagact acaatgttac gagacaaacc gtgtatagaa taaaaaaaga cagcatggtc
     7261 aatgacaaat agttgatcat gctgctttta ttatcttctc atctttaatt tcctacatat
     7321 aattactatc tataaaatta agtcttactc agaacagata aagcataaaa ttccaacaaa
     7381 ccatttttta cttaactaaa taaagagtct gttaatataa tcttcactta tttacgttaa
     7441 tcccctactg tatttgttgg ttccttatct atattttcta caaaatcctt attatcttgt
     7501 gatttagctt cgtataattc tttgtatgtc gacacattgg ttatactaat tcctatagct
     7561 agttcttgag actctacttt tctctctagc tcctttgaca aagtgatttg agatttattt
     7621 ggttccgttg gagtcaattc tatagattct tcataaaaat ctctatacat aaagctatcc
     7681 ctgaataatt catagcttta attctttggc acattttatt gaaatatgta tcactaatat
     7741 ccctatcaac tgattttgac taaaaagtta cttcaaacat tcactgttca aaaatattga
     7801 ggaataaatt ttgggtgtaa ggcattttga gcatacttct ccctggataa cttcccagta
     7861 aaaaaatcgt tagattgttg gattagatcc attgactggc tcgcctcagg cgagcaaaga
     7921 ccctgtagaa ttcattctga tacacatgat aactagataa tttgagatag actcgtctac
     7981 ccgtttggac taatttccca gcaactttca gaaacttcaa tcgaatagaa tgaatggtca
     8041 ttcccttttg gacttcttca aagccaatcg ttcttaagaa gttgactaag ttgtaagcta
     8101 tcaagctgag catcattcgg acatgattct ccaaaaagcg aggactgtct gttttgtcaa
     8161 agtaaaagcc agctttcgct tctttaatga agttctccat tgtgccacgt ttggcataga
     8221 tagaaaagat ggtatcagga gaaacatttt ctgatagatt cgtcacgata aattcatgtc
     8281 gaaagagtag ttcgcccgct tcacgtgttg agcgtataca cacgcgacga ctttgtgacc
     8341 aggattgtgc ttgataagtg gtcgagaagt actgaacttc tcgttcttcc cacttttgat
     8401 tatcgccata aagtactgat ttctcagcta tttgacctag tctacgatta ttcttcagtc
     8461 gaataacata atgactcttt tttgattcac acgaatcata cacaccaggt gtagcgaacc
     8521 cgctgtctcc acgaaccaag atgtcagtgt ttggtagaga gtgattatag tgctctaata
     8581 aaggtgtgag aaactccttt acgccttttg atgtatattg atttcctgaa cgtagttcag
     8641 cttttaagaa gtcaccagtc aatccgtcaa aagctaccaa tggatggtaa ccataggttt
     8701 ggtagtgtgt attataattc gtttgttctt ggtgaccaaa tgtatctgaa tgggtcgaat
     8761 ctaaatcaat gattaattcc gtatcattac ggatgaggcg tgctttatcg ataagttctt
     8821 ggttcaaagc ttgaagttca tggatattct cctcagatag tcgatctaaa aatcgggaaa
     8881 gtgaagattg agaagcgagt tctttcctat ctaaaacagc tttaaacaca ggatcttgtc
     8941 tcaggagatt agctgcagaa tcagctgagt aaccagcaat taattgcata atgaactgct
     9001 caagtatgga taagttgtca tgagtaaaat acgctcgttc gtcttcaatg tgaatgtgtt
     9061 gcttagccag ttcagaaaaa ccaaagatgt tcatcagctc tttcactagg acgagacccg
     9121 aatcactcga taattgacca cctgtatgag aaatagtgat atttgaattg aattttactt
     9181 ggtttttgtg taagctagtc atgaaaagaa ctcctttctt ttggttggtt tagtcacttt
     9241 aaccatagca gaaaggggtt cttttttcat cacttaacgg gtgaagatga aaaagtaatt
     9301 gtaagctgcc gtgaggcagt ttcacatggt tttaattaaa agtatgaatt attcaggcta
     9361 tcattaattt tataaaaaaa attaaatgtt tttagcactg ccatataaca ttcaaaacat
     9421 tcttctatag ttggtttact tccttgagga gaatgaacca atttattacg tttttctatg
     9481 aaatttttaa tttcctttct tatttctttc ggaaactcaa tattaaagta ccctttaaac
     9541 atgctcccta ataatttgtc tacaggggga gattgtaaat tctcaaatat tagagttaaa
     9601 tcaggtttct cttgttgata aaattccttt acccctattt cacaggctgt aattatcgaa
     9661 ataaacctat ctctagtatt tgatatattt ttacttcttt taaggagtat aagagaaaag
     9721 gggatacttt cgtgtccatg catgtaatta actaattgtt gagcttcaga agttatatct
     9781 ctaattatat agttaacgga attaggaagt gtctttaact ctaatgaaat ttctatccaa
     9841 ttactatctt ctaatctgta tatgctcgaa gtataatttg attcgtcagc aagcatcgct
     9901 acatcccaaa cactgatcat aaaatctttt aaacgttcaa tcatttcagc aatattgcta
     9961 aaatcagcta atttttcttt agaataaaaa aaacatttat atcagaatca ataaattcta
    10021 tttttttatt actatatcgt catcattatc ttgaccattt atgtttattg ttccctgagt
    10081 ggggagtact tctttcaaat tcaagttaaa tttatattcc attacttctc ctccaatata
    10141 taaaaattac ttaactgtat ccaaatttaa tatgtaaata aatcattaat ttttagctaa
    10201 attcta
//
LOCUS       DAZBSA010000096         9987 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000096 DAZBSA010000000
VERSION     DAZBSA010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.097"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            122..1243
                     /locus_tag="ACHGMT_002298"
     CDS             122..1243
                     /locus_tag="ACHGMT_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6836019.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQDMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNGVS
                     LARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELI
                     SLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIK
                     NNKDKYIFISKNGNPISIQAYNQSLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPL
                     KAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
     gene            complement(1325..3097)
                     /locus_tag="ACHGMT_002299"
     CDS             complement(1325..3097)
                     /locus_tag="ACHGMT_002299"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6836020.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(3100..4812)
                     /locus_tag="ACHGMT_002300"
     CDS             complement(3100..4812)
                     /locus_tag="ACHGMT_002300"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6836021.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            4927..5595
                     /locus_tag="ACHGMT_002301"
     CDS             4927..5595
                     /locus_tag="ACHGMT_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFN6836022.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            complement(5662..6450)
                     /locus_tag="ACHGMT_002302"
     CDS             complement(5662..6450)
                     /locus_tag="ACHGMT_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN6836023.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6533..8005)
                     /locus_tag="ACHGMT_002303"
     CDS             complement(6533..8005)
                     /locus_tag="ACHGMT_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFN6836024.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            complement(8135..9328)
                     /gene="metK"
                     /locus_tag="ACHGMT_002304"
     CDS             complement(8135..9328)
                     /gene="metK"
                     /locus_tag="ACHGMT_002304"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFN6836025.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     regulatory      complement(9326..9405)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            complement(9594..9665)
                     /locus_tag="ACHGMT_002305"
     tRNA            complement(9594..9665)
                     /locus_tag="ACHGMT_002305"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9630..9632),aa:Glu,seq:ttc)
     gene            complement(9677..9750)
                     /locus_tag="ACHGMT_002306"
     tRNA            complement(9677..9750)
                     /locus_tag="ACHGMT_002306"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9714..9716),aa:Ile,seq:gat)
     gene            complement(9763..9833)
                     /locus_tag="ACHGMT_002307"
     tRNA            complement(9763..9833)
                     /locus_tag="ACHGMT_002307"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9799..9801),aa:Gly,seq:tcc)
     gene            complement(9838..9910)
                     /locus_tag="ACHGMT_002308"
     tRNA            complement(9838..9910)
                     /locus_tag="ACHGMT_002308"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9875..9877),aa:Phe,seq:gaa)
     gene            complement(9914..9986)
                     /locus_tag="ACHGMT_002309"
     tRNA            complement(9914..9986)
                     /locus_tag="ACHGMT_002309"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9951..9953),aa:Asp,seq:gtc)
BASE COUNT         3219 a         2115 c         1658 g         2995 t
ORIGIN      
        1 tagctaaagc tgaacaatat agacgatctt tagaattgtc aaaagattaa atatataaaa
       61 agccttcggg ctttttcttt taacaattaa taggaacgta tattcgaaag gaatagaaat
      121 aatgtggtta gaaaaaataa atgacaaaaa atttaagtac accgaacgtt atacggatcc
      181 cttaactgaa aaaaagagaa aagtgtccgt tactttatct agcaattcac gccaagcttg
      241 gaatcaagct aacttacttt tgaatgaaaa aatcgcagaa aaaataaaac gaaatgaaga
      301 attaccacta acttttggag aattaaaaac taaatgggat gaaaaataca agccaactgt
      361 aaaagaaagt tcctatcgaa caactcaggt ttatctatca ttaatttcaa aatatataaa
      421 agatgatgta cttgtaaaaa acgttaatag taatcttata caagatatgc tagattacta
      481 ctattttgaa aaaaatttat catataatta tgtaatacat ttaaaaactt ttacaggaat
      541 gatttttaaa ttcgccaacc gtcgatatgg attaaaatat aatcccgtaa atggggtttc
      601 tttagcaaga aaacctaaga caaacgaaga aataaaaaaa gaaaaagatg gttatttatc
      661 aaaagaagaa gttcaaatga ttgctagtaa acaaaaaagt tcacatcaac agtcacgtta
      721 ttctttaatt actaaatttt tgtttttaac tggactacgt tatggtgaat tgatatcttt
      781 gactgaatct gattatgatg gtaataaaat tacggtttct ggtacttatg attacgagtt
      841 aaaaattaaa acaacaacta aaaatacagg ttcttataga accattgaat tatcaagtaa
      901 tgctaaagaa atcatagatc aattaattga agaaaacaaa ttaattaaga ataataaaga
      961 taaatacatt ttcatctcta aaaatggtaa cccgatatct attcaagcat ataatcaatc
     1021 tttaagagca gtatccaaag aattaaacct agataaaaaa gtttcttctc acatgttaag
     1081 gcattcccac atttctctac ttacagaatt aggaatccca ttaaaagcca ttatggatcg
     1141 tgtaggacat gaagattcaa aaacaacatt aaagatttat actcatacaa ctaaaaatat
     1201 gcaaaatcaa ctagtagaaa aactaggaaa aatagaaatt taaattgccc cttttctgcc
     1261 ccttataaaa acaaacaaca taaaatatat aaaaagaacc ccgttataac gaggttcttt
     1321 taatttaact ttcaaataca aattgattat gataaagttc cgcataaaat ccattttctg
     1381 ctaataactc gtgatgactg ccttcttcaa tgacttcccc atcacgcaag acaacgattc
     1441 tatctgcatt caagattgtt ttcagccggt gagcaatcac aaagctggtt cttcctttga
     1501 ttgcttcatc cattgctttt tggatccttg cttctgttac cgtgtctaca ttacttgttg
     1561 cttcatccaa aatcaataaa gatggatttg tgatgatcgt tcgagcaata ctgatcaact
     1621 gcttttgacc tgtactgaat aagttatttt cttcggatat ctctgtatca taaccttttt
     1681 ctaacgtcat gatgaagtca tgaatattcg cttgttttgc tgcattgatg acttcttcat
     1741 ctgaagcgtc cggtttgccg aatgtgatat tgtctttgat cgttccggag aacaatacag
     1801 attcttgcaa aacgattcct acatgtctac gtaatgcatc aagatcataa tctcggatat
     1861 ctattccatc gatgactact gaaccatcat tgacatcgta aaaacggttt agcagattca
     1921 tgatcgtcgt tttccccgat cctgttggac caacaagagc aaccatttct cctttgtcca
     1981 cattgatcga gacatctttc aagactagtt tttctggaga ataaccaaaa tctacgtgct
     2041 gcaacgcaag acctttttca acaccatcaa atgctttaca gttttctggt ttgacttcat
     2101 ctttttcatc aaatatttca ttcaatcgtc ttgcccctgt aatcgctaat tggatcatgc
     2161 tgtatccaga tgaaatctgc atcagtggtt ggtaatattg ttgtgaatac tggacgaatg
     2221 tcacaactaa gcctaatgcc accgaacgtt ccaaatcacc atttaatgct aaccagccgc
     2281 cgaaaaagat aactatcgct gtattgacca gtgacattcc ttgcatcatc ggaaatagta
     2341 gaccagaata agcttgtcct ttgaaggttg cttcgcgaac actttgatta tgctctaaga
     2401 atccttcaat cgtttcttct tgtaagccat ttgtgatgat gacacgttgt ccgctgattt
     2461 tttcatccat ataaccattt agtttcccca cttcatcttg ctgaagatcc acatatttcc
     2521 gtgctttgcg gataatcaag acagctatca aaacggctac tggagtggaa gcaattgtag
     2581 cccaagctaa ctctacattt tggcggaaca tcatgatcaa gatcccgaca agtaaaacag
     2641 cattcgtgat gacttgaagc aaagcttggt tcaaactatt ttgaatatta tctaagtcac
     2701 tggtaaaacg acttaaaatt tcaccgtctt gatgagaatc aaagaaacgg atcgtcaatt
     2761 tttccaattt gttgaataga ccgatacgca ttcggttcgt tgacttccct actacttgtg
     2821 tgaacaaaat actatagata aagttggctg cactagctag tacataaaac agcaagagtt
     2881 tccatattac agaaacaaat ttgcttttat cgtctacgcc ttgcatcaag cctcctacgt
     2941 aattagctaa ttcttgaaaa gcttccccga tgtattgagg tgcctttact tgtagatagg
     3001 tagcaatgat cactgtgata aagataaaga aaaaggagag tttgtaccgc ttcaaatagt
     3061 gatagaaaaa tttacttgct tttattagat cagtcatact tactcctccg ttcctttttg
     3121 agtttcaaag atttcctgat agacacggtt tccagctacc aattcctcat gggttccttc
     3181 gccgactaat ctgccttcat ccaatactaa gatgcggtct gctttgacaa cagaagcaat
     3241 tttttgtgcg atgacaatcg tcgttgttcc cttcaaatct cgatcgagtg cttctcggac
     3301 cagcttttca gagcgagcat ccagtgcact cgtactgtca tctaggatca agatctttgg
     3361 attaccgata acgccacgag taatggataa acgttgcttt tgaccaccag aaaagttact
     3421 gctacgttct tctattggtg cttcgtaacg atcagataac ttttcgatga attcttttgc
     3481 ttgagcaata gaagaagcat gttccatctc tttttctgtt gcattctttt tcccttggcg
     3541 caagttttga gcaatcgttc ctgagaataa aattgctttt tgcaaaacaa aggaaaccgt
     3601 ttgtcttaaa ctcttttcat ttacttgacg aagatctaca ccacctactt caactttgcc
     3661 ttctgtcgga tcaaatagac gagggatcaa ttgtgctagt gttgacttcc cggcaccagt
     3721 cgccccgacg ataccaacca tttctccagg tttgatagaa aagctgatat ctttaagcgt
     3781 atctgtatcc tcaccaggat aacgaaaaga aacatggtca aaagtgacag aacccgtcaa
     3841 ctcttgttct ggaacgtcta aataagtaag atctggttca gcatctaaaa tttccgcaat
     3901 ccgcttcagc gatacggctg ctcttgaagt catcatcatc atcattccgc caataatgat
     3961 tgccatcata atctgcatca agtagttcat gaatgaagca attccaccaa ttaacgtagg
     4021 atcatcttta acaagatcgc tcacaaaaaa gatcgcccca accacagcca aattggcagc
     4081 cagcatgaag gacgggatca tcacagaaaa taatgtaccg actatgatgt tgtgttttgt
     4141 taagtcctca cttactttgc tgaatgactt caattggttt tcttcttgta caaaagattt
     4201 cactacacga atccctagta agttttcttt tgcaatgcta ttcacacgat caatcagttt
     4261 ttgaatgatc atgaagtgct tgcccatcct tgtaaatgaa agtgccgtaa tcagaaatac
     4321 cgtaacgata agcgctacga tgatccacca taattgcggc atcgtgatca tagctaaaat
     4381 gaaggcacca ataaataaga atggaattcg aaaaagtgat tgcaaagaaa tcatcactaa
     4441 attttggatt tgcgtgatat catttgttag acgaacaaca agatttccag cagagaactg
     4501 ttcgatattt ccaaatgaaa atgtctggat ctttcgaaat gcttcttctc ggatatctgc
     4561 acttacgcct tgagctactt ttgcagagaa aatcgtgttc gttacacctg cggctaatcc
     4621 tagcaaagcc agactgatca ggtaaatacc aattgttttc atttcatcat tatcttctgt
     4681 aataatagct tccaatactt gctgtagtaa cttaggttgc cataatgctg ctgcaaccat
     4741 gactgctacg gacagcaagg aaataaaaac agctacttta tattttttcg catgtcgtaa
     4801 gattaatttc attcgttgat tacacccctt tgtttaaatt gacaggacta atttaacact
     4861 gtaaaattag accgactaac aatttaccat gtttaaaaag ataatgcaat acacgaggtt
     4921 gataaaatgg aaataaagct ttctcaagca atcatcattc aggccgcctt tgatatcctg
     4981 gctgaaacac aagctctttc gcagctttcc atgcgaaaca tcgcaaacag aattcatgtt
     5041 caagcacctg ctttatattg gtatttcaaa aacaaacaac agctcttgca aataatggct
     5101 gaaacaatgg aagaagaatt agcgcttcca gatccaactt tgccttggaa tgaacagttg
     5161 ctgcagtata tggaaaatta ctatgacctt tatacacgtt tcccttgtgg ggcagaatta
     5221 gaaatcaata ctgtgcccgc ttttccgagt cgattagaac atttggagca aatgatccaa
     5281 ttacttgttc gtgaaggatt ttcagtcacc caaagtcatc aggccattgc ctctctccac
     5341 aatttattga ttggacagtt aatggatcag cagcatgaac agcagcttcg acaaaaaatt
     5401 atggacggaa atgaattttt gaaagaaaca gttctattta tgcgtaatta tgtagttgaa
     5461 aatcaattag agggatttaa agaaagtatg cagtcccatg cagaattgaa tgaaaaagca
     5521 ctctttctaa cgagtgcaca aacctatatt gaaggcttat gccaacaaaa taaaagaaga
     5581 gaaaacgagc agtaaaaaag ctcctataga gatcactatg tttgtgatct ctataggagc
     5641 tatcttttta agactctttt tttatttttc ttccattagc cacgccaatg cacgttgaat
     5701 ccattggtcc cagaagatcc agttatgatc gccaggtcct tgttcaaagg tgaccgccaa
     5761 gccttgttcc tgcatagcag ataccatttc ctgatttgcc tgataaagaa aatcagattc
     5821 cccacaagcc atataaaatc ttggaactgg ttttccgctt tttactagtt ctttcaataa
     5881 gtattgcgga tcattttttg aaccttcgat ttgatccaat ggtccaaaga tcccttccca
     5941 aaaagatgct ttacgaacca tcaaaagatc atccattcga tttcctacag aaacagcgcc
     6001 tgaaagagag attactgctg cgaaattttc tgattttgca aggcctagtt ttaacgcgcc
     6061 atatccgccc atcgataatc cagcggcata tgttttttct ctttttcttg ttaattgcgg
     6121 gaataattca tgacaaatct caggtagctc ttctgcaatg aatgtccaat acttcatgtc
     6181 ataaactgta tctgtgtacc agccaagatc agtagatggc atgatgacag ctaatccgta
     6241 gtttgctaca taacgttcaa tcgacgtacg tctttcccat acactatgat tgccacccat
     6301 tccatgaagt aaatataaaa caggtatatc cgttgtctct tgctctgaac tggtgccaat
     6361 cacctttttt gttgtttgcg gtagtatcac atttagattg acttccattt ctaacacatt
     6421 cgaataaata tttgcttgaa taaatgccat attttcgcct tccttagtaa gaagttattt
     6481 ttgaataact ttaaatttga atgattcggc tacgtacata tcttaaccga aactactgtt
     6541 ttattttacc acttttttca tgaacgaaaa gcgttaagac caaagcaatg atcacgagta
     6601 acaagccaac tagataaacg gaatgtaacc catcataaag aatttttctt aatggtaaca
     6661 aaatatccgc atcaatatat ttagccgttt gtggattcac cagtttattc atgatatctg
     6721 catcatttgt taaatttgct gctgcaagtt ctcgtttagt taccccattt atcaataaac
     6781 caaaaataga aaccatgact gtttgaccaa ttgttcgaac taacgtatta aaagaagttg
     6841 ctacaccaag ctggtcacga gatacactat tttgtgcact gacagtcgag gtagtcatgg
     6901 tcacacctaa gcctataccg atgaccgttg atatagcaaa aaaccataaa tagttacttc
     6961 tgaaaggcat aagatacaac caagcgcccc caatagctgc gattagtagt ccaattctca
     7021 agacccaatg agttttcatt tgagtcatcc acttgcttgc aaggaaagaa ccgaccatcc
     7081 agactagtga taatggtgct aataccagcc caccaattgc tgcattcagc cctaatacac
     7141 cttgcatcca catagggata taaacatcga ttcccatcaa aaaaccacta gccaatgctg
     7201 cagccaagtt gaccacaaca aatagtggat ttttgaataa atgcatcatg atgacaggat
     7261 ctttagctct tttttcggca aaaacaaaaa tacctaacat tacgatacta cctagcagca
     7321 aagcagaaac aagcggactc attccttcgt ctcctaataa ttggaaccct aacagcaatg
     7381 agacaagcat ggccatcaaa ctaaaactac caaaaatatc cattggttta gactcatgta
     7441 tgatttttgg ttcatttaaa tatagccaaa tcaaaataat taatagaatt ccgatcggca
     7501 cattgataaa aaagatccag tgccagctga tcgtttcaac gatgaatcct cccgcaagcg
     7561 gtccaaaaac actggcaatc ccccaggctg aactattcaa acctaaaact tttgctcgct
     7621 tttcaataga ataaagatcg ccgatgatcg tcaaagctac tggcatcatt gccccagcac
     7681 ccatcccttg aatcgcacga gcagcaatca acccaatcat gctttgagat aaaccgcaaa
     7741 ataacgaacc gataataaac aataatgtcc caaaaataaa gattggtttc cgtcccactt
     7801 tatccgcaag ttttccgtaa atcggagtaa gcatagcgtt tgtcagcaaa taaatcgaaa
     7861 atacccagtt catgatttcc attccatgta atgaaccgac aatcgttggc atagcggttg
     7921 ttacgattgt accttcaata gccgtcatga acgttgcaat gaaaatactc actgtgacca
     7981 aagtaacatt tgtttctttt ttcatcttat tcctcccttt cattatgaca ggatctaaaa
     8041 atagataaat aattactatc tttttcaaga ggttaactgc cataaaaaaa gctggaacaa
     8101 ccaccacttg gcgatcgttc cagctgaata taacttattt tgctaaactt gctttcaacg
     8161 catctacttt gtccgtatgt tcccaaggca aatctacatc tgtacgacca aaatgaccgt
     8221 aagcagctgt ttgtttgtaa atcggacgac gcaaatcaag catctcgatg attcctgctg
     8281 gccgaagatc gaagttttct ctcactgcag caatcagttc ttcttccgga accgttccag
     8341 taccgaaagt attgattgag atcgaaacag gttgagcaac accaatcgca taagctagct
     8401 gtacttctgc tttgcgagca agccctgctg caacaatgtt tttagcaata taacgtgcag
     8461 catagctggc agaacggtca acttttgtcg catctttacc agaaaaagca ccgccaccat
     8521 gacgagcata accgccatac gtatcaacaa tgatttttct tcctgttaat ccggcatccc
     8581 cttgaggacc accaatgaca aatcggccag tcggattgat ataatatttc gtttgatcat
     8641 ctaataattc agctggaata acttccttca ctactttttc gatgacatca tgacgaattg
     8701 tttcattatc tactgcatca tcatgctgtg ttgaaataac gattgtatct acgcgttccg
     8761 gttgtccttg atcatcatat tcaaccgtta cttgagattt tgcatctgga cgtaaatacg
     8821 ttaattcatt tgatttacgc aaatctgcca agcgtcgtac cagacgatga ctcaaagcga
     8881 ttggtaaagg catcaattct ggtgtttcat ctacagcaaa cccaaacatt aaaccttggt
     8941 caccagcacc aatctcatct aatacgtctt ttttatcttc atcccggatc tcaagtgctt
     9001 catcaactcc ttgagcaata tcaggagatt gttcatcaat cgcaaccaat acagctgctg
     9061 tatctccatc aaatccgaat tttgcacgtg tataaccaat ttcttttatt gtttcccgta
     9121 cgactttttg aatatctaca tatgccgtcg tagatatttc tccaaatact aaaacaagac
     9181 cagttgttac agacgtttca cacgctactc gtgctgttgg atcttgtttt aaaattgcat
     9241 ccaagattgc atcactgatt tggtcagcaa ttttatctgg atgtccttca gaaacagatt
     9301 ctgatgtaaa taagtgtctt tctaccatta taatgattcc ccctagttaa attcggttac
     9361 aaggcatctc cgtcctgcgg atcctttcgg gaacttgcaa cgaaccaagt atagcagata
     9421 aaacagtttt ctgctcgtat tttttcattt ttttgcttaa aaaatataaa aatattttac
     9481 attagaactg tccaaaaaag cttagcacaa tcatccataa agataactct gctaagcttt
     9541 ttaaaatcat tctttcagat aaaaagaaaa agatactcaa aaagtatctt ccatgacccg
     9601 tacgggattc gaacccgtgt taccgccgtg aaagggcggt gtcttaaccg cttgaccaac
     9661 gggccaataa aataaatggg cctaaatgga ctcgaaccat cgacctcacg cttatcaggc
     9721 gtgcgctcta accagctgag ctataggccc ataaaaacta aaagcgggtg acgagaatcg
     9781 aactcgcgac aacagcttgg aaggctgtgg ttttaccact aaactacacc cgcataatgg
     9841 cgcgggacag aatcgaactg ccgacacatg gagcttcaat ccattgctct accaactgag
     9901 ctaccgagcc aaacggttcc gacgggaatc gaacccgcga tctcctgcgt gacaggcagg
     9961 catgttaacc cctacaccac ggaacca
//
LOCUS       DAZBSA010000097         9872 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000097 DAZBSA010000000
VERSION     DAZBSA010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.098"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(338..562)
                     /locus_tag="ACHGMT_002310"
     CDS             complement(338..562)
                     /locus_tag="ACHGMT_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836026.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            753..3530
                     /locus_tag="ACHGMT_002311"
     CDS             753..3530
                     /locus_tag="ACHGMT_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFN6836027.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3611..4030
                     /locus_tag="ACHGMT_002312"
     CDS             3611..4030
                     /locus_tag="ACHGMT_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN6836028.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4042..4512
                     /locus_tag="ACHGMT_002313"
     CDS             4042..4512
                     /locus_tag="ACHGMT_002313"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN6836029.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPAEKKVDVREVL"
     gene            4529..5308
                     /locus_tag="ACHGMT_002314"
     CDS             4529..5308
                     /locus_tag="ACHGMT_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFN6836030.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5295..6116
                     /locus_tag="ACHGMT_002315"
     CDS             5295..6116
                     /locus_tag="ACHGMT_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN6836031.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6133..7182
                     /locus_tag="ACHGMT_002316"
     CDS             6133..7182
                     /locus_tag="ACHGMT_002316"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFN6836032.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7195..8199
                     /locus_tag="ACHGMT_002317"
     CDS             7195..8199
                     /locus_tag="ACHGMT_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFN6836033.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8832..9851
                     /locus_tag="ACHGMT_002318"
     CDS             8832..9851
                     /locus_tag="ACHGMT_002318"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFN6836034.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3310 a         1642 c         2065 g         2855 t
ORIGIN      
        1 gtggagaaaa ctatggtttg gcgcagattc tatcaggttt gatggaacga agacaacctc
       61 ccgacatcaa gtcaaaagcc acttgagatt ttgatctgaa aataaaaaaa gaaaacaagg
      121 taggaacatc aatgtttcta ccttgttttc gtaatcaatg ggaaaaaact aagagtggtg
      181 tacgtaatca ataaaaagga tggtgacaac agtacctctt tactctctga aagcatcata
      241 actcgttttt cctcattatg ccaataaatt gttgttatac cacatataag gatttgtaat
      301 agctaaaaac agacaggaaa gttgaaatct gtgaatctta ttttgtatgg aaggatttat
      361 agaacgcaaa aatcaagata aagatgaata aaaatcctgt ttcggctata tattgcgtat
      421 ctacgccaat aaaacgagtt tgttctgtca tagccatata aagcaaaggc aaaaatacaa
      481 ctacagcgag ataagatccg atgatttctc gaaaagtttt ccaggaagta tgtttcctgt
      541 ttttatattt atccgatttc attgaatagt catagcctcc ttattctttc atatgttgaa
      601 attccagtat aaaaactgga aggaactgta cgttttttag aataaaaatt ataagggata
      661 agtaggctgc tgtctataat aagatatatt tttcaattct ttattaaaga ttttattgac
      721 aaaatgaatc cgttttctat gatggaggag gggtgaataa gttgttgaag aaggaaattt
      781 tatgttactt agagaaccag acagcctttt tcgatttaga acatatgaac gaggtcttta
      841 cagcacaaag gttagctgat cattttgggg taaaaagaaa cacagtcagt cattatttga
      901 accagttaaa tgatgagcaa ctactagtca aaatcaattc tcgaccagtg atttatttcc
      961 acaaagaagc ttttgaaaaa caatttttta agttacgcac gaatttttat ttttctatcg
     1021 atgatctcaa agaggaacag ccttttttcg cgcaaccaaa agacttattc tcttttatga
     1081 tcggatacga cgccagtttg aaagaaagta tcgaacaaat caaaacagct ctttactatc
     1141 cagatggcgg attaccactt ttgattacag gcgaaagtgg cacaggtaaa agttatttag
     1201 ttaatttggt ctatcagtat tgtctgcttc atgatctgct agaagattct gcaccattca
     1261 tcacttttaa ttgtgcacaa tatgcagata atcctgaact tttgactagc aatttgtttg
     1321 gacatgttaa gggagcgtac actggtgcag aagaaaataa aaaaggggct tttgaagcag
     1381 cagatggtgg gatcttgttt ttagacgagg tacatcgctt aacaccagaa ggtcaggaaa
     1441 aactgttcac ctatcttgat caaggtgtga tttatcgtat gggagaacct aatttgtcac
     1501 gccggatcaa aacacgtctc ttttttgcta cgactgaaga gattactagt catttcttaa
     1561 caactttcat tcgaagaata cctatccgaa tcgaactacc tgcacttaat cagcgtagtc
     1621 gtaatgaacg gctagaactg atttactctt tttttctcga agaacaaaga aaaatgggga
     1681 gccctttgtc agtcagtgga ccagctttgt cacttttaac aagtcagaaa ttatccggaa
     1741 acataggaga gctgaaaaat atcgtcaaag tttcagcagc aaaagcatat gctgaacagc
     1801 gtgaacagcc tgaaatccat gtcacgatcc atcatttgca aaaagaacta cttgcgcagc
     1861 ctattactaa aaatacagca cagcaaggaa tctatattac gcaagaccag accttagagc
     1921 aattaatgga gaagcaacag cccgaacagc agcgaatcgt taaaagtttt gagcaaatcc
     1981 taattgattt caagaaaaat aactgtctgc tatattcttg cgaaggaaaa ttaaagcaag
     2041 aagtgattca attatttgat tttttgctgt ttgagaccag tagacaagaa aaacacgagc
     2101 tgctggttta ttggacacaa tctatccgag agacctttcg acaaatggaa actgcctatc
     2161 aaatcaaatt cgatggaaac agtgtctatg ccttgagtta ctatctctat cagcgaagca
     2221 ctatcaaatg gttaccggaa gaacatgata ttatccaact aatcaaagaa ttagaacaac
     2281 aagtttctca gtcttatcca tctagttatc actatgtcca acgaattctg gaattaagta
     2341 agatcaaatt agatattgaa gacacaagta tggatcggat tttactgacg atctatctaa
     2401 aaaaagcgaa atggtcaaaa gaaaaacgac tgccaaaagc gatgattgct gctcacggtt
     2461 atgcaactgc tagcagtatg gcaaatgtag taaatcgttt attacaagag gatctatttg
     2521 aatcatttga tatgccacta gatgtcacac cacagcagat tgcttcagaa atccttgatt
     2581 attgcgaaaa cagcgatgtt tcaaatggat tgatcatatt agtagacatg ggatctctga
     2641 aagaaatcca tcagtttttt aaaaaacagt tatccgtacc gcttttgatt ttgaataatg
     2701 tgacgacacc tttagcaatc acagtagggg aatgcctgca aaaaaatgta tcattagaag
     2761 agatagcaga agagactgtt cgacagatcc agccagaatg gcgactgctc tatccagagg
     2821 aaaacaaacc caaagcactc attactactt gcttcactgg aatcggtacg gcggtccact
     2881 taagtgaact gctagaaaaa agcttaccta cggcttgtca gttgaaaatc atcccatatg
     2941 aatatcagca attaaaagat aagaaaaaca gtgacccatt attttccata tacgaagtcg
     3001 taggaatgat tggcaccaca gatcctgata tcaccaatat cgactatctt tcattagaag
     3061 acctcatctc gggggaaaag atgtctgtac tagctgaatg gttggacact actatgaatg
     3121 cttcagagaa ggaaatattc aatcagcgca tcattcgtaa tttttcattg gaaaaagtac
     3181 tggattccgt gactattcta gatacagaga aggtgatggg agaaatcgat cattttatga
     3241 gagaattaga ggtgcagttg aatcgaagca tcagcaatcc caaaaaatta gcgttgtatg
     3301 tccatgtgag ctgtctcatt gaacgattga tccgacaaat gccaatcgaa acctatcaag
     3361 gattagagaa atggaagcag tgtcaaaaag aagggttatc tgctattaaa tctgcgttta
     3421 gtgtcattga agagcattat agtgtcatga tccccaattc tgagattgcc tatatctatg
     3481 atattttgag tgaagataca gagtttctct cgatagaaga agaattttga acataagcaa
     3541 gttgttgcca ctaattttgg catgcaactt gcttttttag tatgtgtaag tgaaaagagg
     3601 agatgaaaaa gtggatagag aaattatctt agcttcccat ggaaaatttg cttcagggat
     3661 attagattca ttagagctga tctatgggaa aaatcattct attactgtat tcgattgcta
     3721 tactgatgag aattttgatt tagcggaaac agtaaggcag ctttttatga ggtacaaaga
     3781 taaagaaatc attgttctga cggatctttt tggcggaagt gtcaacaacg aattcttaca
     3841 atatatcaat cgggatcatg tctatctcgt tgccggattg aatttgccac tattgattga
     3901 gctagtatct cggttggacg ctgaagtaga gacaactgtt ttgatccagc aggtgctaac
     3961 tgattcaaaa aaaatgatcc agttttgtaa cgagcggctc aatcagaaaa tagaagaaga
     4021 cgaattttag gaggaagaaa aatgattgta ttgacaagag tcgaccatcg gttattacac
     4081 ggacaagtag ctttttcatg gacacaaaca ataggcgcag actgtatttt gattgctaat
     4141 gatgatgtac ctacgaatga gatacgaaag acaacgatca aattagccaa gccacaagga
     4201 gtcaaactag tgatcaaatc aattgatgac tcgattgccg cgttgaaaag cggggtgaca
     4261 gataaatata agttattcat cgtagtggaa tcgatcgagg atgcatataa gctggcagaa
     4321 gcgtatccag aaatcaaaac catcaactta ggagggatca aggcaaaacc tggtactcgc
     4381 agcatcggaa aagcggtaaa tattttacca gaagaggagg aacgcttgaa acgattaatt
     4441 gacaaacaaa tagaagtaga aatccgtcaa gtaccagcag aaaaaaaagt ggatgtaaga
     4501 gaagttttgt aagaaaggaa gaacctcaat gattgtacaa gcagtcttat taggaattgt
     4561 tgcatttgtc gcacaatcag aatatgcact agggacatcg ttgctttctc gtccgattgt
     4621 gacggggctg tttacaggaa tcgtgttagg tgatgtaaaa actgggatca ttatgggcgc
     4681 aactttagag ttagcattta tcggatcgtt ctcagtaggt gcttcgattc cacctgatgt
     4741 tgtgaccggt gggattttag ggacggcttt tgctattaca gctggagccg gtacagaaac
     4801 cgcgctttta ctaggtttgc cgattgctac gttgacattg atattgaaaa atatttactt
     4861 agggcttttg attccaatta tgaaccataa agcagacaat tatgcagaag aaggaaatta
     4921 taaaggagtc gagcatatgc acttgcttgc cggtatcggt ctttccttga tgcttggtgt
     4981 tgtggtaatg gtttcttata tggtgggaag caatacgatc ggaaatctat taaatatgat
     5041 tcctgacttc gttcaaaagg ggttgtcagt agctacaggg ttgattccag ctcttggttt
     5101 tgcgatgctt gctcgtttgc tgatcaacaa acaagttgca ccatattttt tcttaggttt
     5161 tgctatagct tcttacttag agattcctgt gaccggagta gctatttttg gtgcgatttt
     5221 agcagttgtg gtagtcaata ttatgaatgt ccgtcaacta ccacttcaaa caactgcagg
     5281 ggaggtagaa gaagatgaag aattctgaga tcaaaaacag caaaataacg aaaaaagagc
     5341 taaatcacgt attttggcgt tcttttcaaa tggagttttc ttggaattat gaacgtcaga
     5401 tgaatatggc ttatgtttat gcaatgattc cgattttgaa aaagttatat cagtcgaaag
     5461 aagaaatggc agcagctttg aaacggcatc tagagttttt taacacgacc ccacatatcg
     5521 ttacactgat tttaggaatc acggctgcaa tggaggaaga aaataaggat gatcctgaat
     5581 ttgatgtgac tgcgatcgat agtatcaaaa catctttgat gggacctctt gcagggttag
     5641 gagattcttt cttttgggga actttgcgat tgattgccac aggtgtgggt acctctttag
     5701 ccatgcaagg aaatatttta ggccctatct tgttcatttt gattttcaat attccgcaca
     5761 ttctgtttcg ctatctagca acaggttggg gatatcgctt aggaacgggt tttttgaaaa
     5821 agatccaggc aaacggtatg atggaaagtt tgacattagg cgcgtctatt attggactga
     5881 tggtcgttgg agcaatgact gccacgatga tcaccatcaa tattccttta aaaatcggtt
     5941 caggagaaaa tgctgtgact gttcaaagta tttttgatga tatcgttcca aatatcttat
     6001 gtcttggagc atttggcata gtgttttatc tattgaagaa agaagtgaaa ccattaacga
     6061 tcttgatcgg actagctttc tttggaattt ttggttcgtg gatagggatt ttttaaaaaa
     6121 ggagcagaga aaatgtttac gatgcaagat tatatctatg aagaaaagga agtactaagt
     6181 acgatcttga aaaagaatga tttttcgaca agagaacata tgaaaaaaac agtgaattta
     6241 ctgatcttag caacgggttc atcttataac gcttgtttag cagcgaagcc agcattggaa
     6301 tcttatggtg atcttactgt cgatattcaa gaaccttttt attttttcaa ttatggaaaa
     6361 ctttctcctt caatcgatac tgtgattgct gtgtcacaaa gcgggaaaag tgcttctact
     6421 gttgaagcag taaaaatgat acaaaaacaa gggttacctg ttgttgctat cacaaatgac
     6481 gtgcaaagtc cacttgcttt agaagctgca caaattattg atttaggtgc cggggtagaa
     6541 tcagtaggct tcgttacaaa aggctattct gcgactgttt tacagttact actcttaggg
     6601 ataggaattg gaataagtaa aaataaaatc agcaaaaaaa tagaacaaga ctacatgcaa
     6661 cagttgcgca aaataatcaa ccatttacca gcgattattc aaaaaacgga agaatttttt
     6721 gatggatatc aaagtttatt tcgtttagca cagcgtttcg tcaccattgg atatggtcca
     6781 aactggggaa cagctaaaga agcagagacg aaattgactg aaacaatccg cgttccgtca
     6841 caaggatttg agttagaagc ttatatgcac ggcccatacc tggaggcaga tgcttcgcat
     6901 ttgctattct ttattgaagg ggaaagtgta aataaggaac gttcgcaaaa gttgcaaaga
     6961 tatatgagtc ggtacgttgg agagactctc accattacca caaaaaaagc aagaaacgag
     7021 aaaacacttg gattagcaat agaatgtgat gaatatctgt ctgtattagc gcttgtcgtt
     7081 ccgtttcaac tattcgctta taaaaccgca gtagctaaag gtattgactt gaacaaaaag
     7141 atttttgaag actttgatac agtcttgaaa agtaaattat aagaggtgga aatcatgctg
     7201 aaattcaatg aagaagaaca aatcaaagca ataaaaggcg cactagcttt acgtccgcaa
     7261 gtagaagaaa tcatcgataa actttatgcg gaaaaatttg acgctgtcta ttatctagga
     7321 atcggtggaa cgtatgcttc ctcgatgcaa gctgtcactt atatgaatgg gaaaagcaac
     7381 ttgcctgtct ttgtacagca tgctgcagaa tattacacta ctggaaacaa aagattaaca
     7441 aaagattcta tcgttatttt gtcctctgtt accggtacaa ctcaagaagt agtgaaagca
     7501 gtagaacaaa tcaagaaagt aggagcgaca ctgattggat ttatcgataa agcgaacagt
     7561 aagttgtctc agctatgcga ttttgtggtc acttatccag ctcctggaac agaacaaatc
     7621 aaatttttta tggcagcaga tcgattgatg tatttgaatg gagaattcga agcttattca
     7681 gaatactatg aacagctcga acgttattta ccaacagggt tagtggaagc agagaaaaaa
     7741 gcagatgcat ttggcttatc atttgcagaa aaacatcgac gtgatagtat gcattatttt
     7801 attggagcag ggaatcaatg gggagctgtt tattcatatg ccatgtgtta ctgggaagaa
     7861 caaagctggc ttccatcgaa atctatccat gctgccgagt ttcttcacgg gacgttagag
     7921 atcgtagaag aaacaacccc agtcacatta tttttaggcg aagatgaaca gagagtgtta
     7981 tctgaacgtg tggctaaact tttgccaaaa atttgcagca attatacatt gatagacaca
     8041 aaagactatc cagtagaagg tatcagtgaa aaatatcgcg gacgtgtttt gtctttctta
     8101 ttgatgcatg tagtgacaca acgcatcgat gctcatgtgg agcagttgaa ctgccatcca
     8161 ttagagatac gccgctacta tcgtcagttt gattattagg atagactgga taaaaagaat
     8221 ataaaaaata aagagagcag cgataactgc tccctttatt tttttaaaat attggcatca
     8281 attacgttat aaaacacatt ttttgcatta tctacagttc aacttataat ctgtttaatg
     8341 ggacatgtgg gtgccaacca ttttttgtta tgcaatagta ctatttagtt gttgcatgga
     8401 tccgtagtaa aagtccaagt acaacgagtt gcgccagttt acatagcgtt ttgtatccag
     8461 aatgttgact aaaacaaaag ttcctatgtc aaccgtcaac agagacaatc tgtccgttgc
     8521 aggacctgac agatttagaa atgttcaatc tgtctcttat gtattttaaa tcaatactca
     8581 ctaggagaag tttcctaacc tcctaatagt aatgctggta gtaacattgt caaaactgct
     8641 tcctcttagg aacaagcaaa gcataacgag tgtaaaaata aaatgtagac tattgtaaca
     8701 tatcattgat tatttcaagt aaaaacgata aaagaagtaa acaaacactt aaaaacaatg
     8761 gaaattagtc ccaactttaa gaaatgtata ctataatgga aattatcaat tatttatttg
     8821 gaggaatatc catgaaacaa aaaatcgcag ttcttggtcc tggatcttgg ggcactgctt
     8881 tagcacaaac attggcagaa aacgggcatg atgtccgtat ttggggaaat gtacctgaac
     8941 agattgatga aatcaataag tatcatacaa atcagcattt tttacctgat ttgacgatcc
     9001 cagaatcgat tatcggatat aaagaattgg cagaagcagt ggataacgca gatgctattt
     9061 tatttgttgt tccaacaaaa gcgattcgtt cagtcgcaca ggaacttatt ggtaaaatga
     9121 atactaagcc aatcattata catgcaagta aaggactaga acaagatacg cataaacgaa
     9181 tctcagaagt tttggctgaa gaaattccag aagaaaaaag acaggcaatt gttgtattat
     9241 ctggtcctag ccatgctgag gaagtggcag tacatgatat cacaacaatt acagctgcaa
     9301 gtattgatca aaaagcagca gcctatgtac aaaaactgtt catgaatgat tatttcagga
     9361 tttatacaaa taatgatgtg attggcgtag aaactggtgc agcactgaaa aatatcattg
     9421 caattggtgc cggcgctatc catggattag gattcggaga tgatgcaaaa gcagcaatca
     9481 tgactcgagg attggcagaa atcagccgtc ttggtgtcgc gatgggtgcc aatcctctga
     9541 catttatcgg tttgagtggt gtgggggact tgatcgttac ttgtacaagt gttcattctc
     9601 gcaattggcg tgccggtaac ttgttaggac agggacataa gctggaagaa gttctagaaa
     9661 acatgggaat ggtcgttgag ggtgtggcaa cgacaaaagc agcagtagag ctagctcaac
     9721 agctaggtgt tgagatgcca attactcaaa caatctataa tgttttgtat aatggagaag
     9781 atatcaaaaa agcagcaaaa gaaatcatgt tacgtgacgg aaaaatggag aatgaatttt
     9841 ctcttcgtta aatagatgat caatagttaa tc
//
LOCUS       DAZBSA010000098         9849 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000098 DAZBSA010000000
VERSION     DAZBSA010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.099"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            159..896
                     /locus_tag="ACHGMT_002319"
     CDS             159..896
                     /locus_tag="ACHGMT_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFN6836035.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            910..1725
                     /locus_tag="ACHGMT_002320"
     CDS             910..1725
                     /locus_tag="ACHGMT_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFN6836036.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1738..2385
                     /locus_tag="ACHGMT_002321"
     CDS             1738..2385
                     /locus_tag="ACHGMT_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFN6836037.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2401..3057
                     /locus_tag="ACHGMT_002322"
     CDS             2401..3057
                     /locus_tag="ACHGMT_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFN6836038.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3219..4040
                     /gene="racE"
                     /locus_tag="ACHGMT_002323"
     CDS             3219..4040
                     /gene="racE"
                     /locus_tag="ACHGMT_002323"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFN6836039.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            4043..5398
                     /gene="rph"
                     /locus_tag="ACHGMT_002324"
     CDS             4043..5398
                     /gene="rph"
                     /locus_tag="ACHGMT_002324"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFN6836040.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5399..5917
                     /locus_tag="ACHGMT_002325"
     CDS             5399..5917
                     /locus_tag="ACHGMT_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFN6836041.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            6024..6524
                     /gene="cbpB"
                     /locus_tag="ACHGMT_002326"
     CDS             6024..6524
                     /gene="cbpB"
                     /locus_tag="ACHGMT_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFN6836042.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(6804..7724)
                     /locus_tag="ACHGMT_002327"
     CDS             complement(6804..7724)
                     /locus_tag="ACHGMT_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFN6836043.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            7850..8902
                     /locus_tag="ACHGMT_002328"
     CDS             7850..8902
                     /locus_tag="ACHGMT_002328"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFN6836044.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            complement(9012..9767)
                     /gene="fabI"
                     /locus_tag="ACHGMT_002329"
     CDS             complement(9012..9767)
                     /gene="fabI"
                     /locus_tag="ACHGMT_002329"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFN6836045.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
BASE COUNT         3210 a         1715 c         2103 g         2821 t
ORIGIN      
        1 agaggataaa ataactaata gattagaaat ggaagaaata tttatgggag aataaacgtt
       61 ctaagaaact ttacaaatta ctaaactatc agatataatg tattttatta tacatcttta
      121 aaagaaatct tactaaataa taatgggagt gtaattctat ggcaatgatt aaatttgatc
      181 acgtggataa atattatggc aagttccacg cattaaaaaa tatcaatctc gaattcgaga
      241 aaggagaagt tgtcgttgtc attggtcctt ctggttcagg aaagagcacg atgcttcgct
      301 gtatcaatgg attagagacg atctcttcag gtaagctgct gattaatgat actgacttgc
      361 atgataaaaa gacaaaactg acagaagtcc gaaaaaacat cggcatggtt ttccaacatt
      421 tcaatttgta cccaaataaa actgttctgg aaaacatcaa attagcacca atcaaagtgt
      481 tgaagcaaga ccaggcaact gcagtcaaaa acgcggagaa atttttgaaa actgtcaata
      541 tgttagataa gaaagattct tatccttcca tgttatccgg gggacaacag caacgggtgg
      601 caatcgccag aggtttagcg atgaatcctg agatgctgct gtttgatgaa cctacttctg
      661 ctttggatcc ggaaatgatt ggtgatgtat tagacgttat gaaaaaattg gctcatgacg
      721 gtatgtcaat gattgtcgtg acacatgaaa tgggctttgc caaagaagtg gcggatcgtg
      781 ttatcttcat ggctgatgga gaagtattgg aagacagcag aaatgtgaga gactttttcg
      841 aaaatccaaa tgaagtccga gcacagcaat ttatcagtaa agttatcaat cattgatagg
      901 aggaaaaaga tgcgcaggtt aaaaaaaata cgcctgattt tccttttagg cattgctttt
      961 cttgcactag ccggatgtaa aggaaatggg ttggcaaatg aagatatttt agagcgaagc
     1021 aaatctacca atgaaatcat ctggggtgtc aagtatgata ctcgattatt tggaatgatg
     1081 gatattgaga gcagaactgt acaaggattt gatgtagata ttgcaaaagc aatcactaaa
     1141 aagatccttg gtgacaatgg aaaaacagaa tttgttgaag tcacttctaa aactcggatt
     1201 ccattattga aaaacggaaa tatcgatgca attattgcaa cgatgacgat cacagatgaa
     1261 cgaaaaaaac aggttgactt ttcagatgtc tatttcgatg ctggccaagc tttgttggta
     1321 aagaaaggaa gccaaatcaa aagtgtagat gatttgaatg cttctacaac ggttctagca
     1381 gtcaaaggtt ctacatcggc tgcgaatatc cgacagcatg caccagatgc aaaaatttta
     1441 gaattagaaa actatgcaga agcatttact gctttacaat ctggtcaggg agacgcgatg
     1501 actacggata atgcgatttt gttagggatt gcagatgaaa atccagagta tgagttagtt
     1561 ggtggaacgt ttactaatga gccttacggg attgcgatca acaaagggca agaaaacttc
     1621 ttgaaagctg ttaaccaagc gctagaggaa atgcatgcag atggcacata cgataaaatc
     1681 tatcaaaaat ggtttccaaa tgaaactgaa ggaaaagtag agtaaggggg agaggcaatg
     1741 agcgaattga tacaaactta tggaccaatc tttgtcgacg ggttcaagat cacattgttt
     1801 tcgagtgtct tagcacttgt gtttagtttg attatcggaa cgttaatggc aatcttccaa
     1861 ttatcaaaga ataaagtagt gagcgcctta gcaaaggcat atgtagaatt ttttagaaac
     1921 attccattat taatcatcgt gatgtttttt tatgttgtcg taccgattta ttggtttaat
     1981 ctagatggat tccaagcagg tacgattgga ttaacgatct atacatctgc ttttattgct
     2041 gaaacagtac gttcagggat tcaaaccgta ccaaaaggtc aaacagaagc aggactatct
     2101 tctggtttta cctattcaga aatcatgcgt tatattgttt taccgcaagc attcaaaatc
     2161 gttatcccgc cattaggcaa ccaatttatc aacctcgtaa aaaactcatc tattttagca
     2221 atcgtagctg gtttagattt gatgtatcaa ggagacttga tcgctagtga aacattcaat
     2281 accttcgata cgtatatcat cgttgggtta ttttacttag ttatcacgct accgttatct
     2341 tacttaatgg tttatcttga gaaaaaatgg gcggttcgtt catagaagga ggggaaatac
     2401 atggatttta gcggcgcttt ttcatggatg aatatccgtt ttttattgga aggattaaag
     2461 gttactgttg aagtttcatt gttttcgatc atttttagtt tcttgatcgg tggtctgaca
     2521 ggtgtgattc gttttgcgaa tatccctttc ttttccaaaa tccttggtgt agtgattgat
     2581 attatacgga atcttccatt attattgatc attttcttca cttactttgc cttacctcaa
     2641 atcggcattc agatgaacat cttctggtcc gctgtcgcag cattaacgat ttttgagtca
     2701 gctatgttgt cggaaatttt ccgagctgga ctcaatgctg ttccaaaagg acagatggaa
     2761 gcaggattat caacgggact gacttatgta gaatcgatgc gaacgatcgt gttaccgcaa
     2821 gcatttaaat caatgatccc ggcaattgtg agccaattga tttcactgat caaagatact
     2881 tcattagcag taatcatttc tttacctgaa ctttctcatc aggcacgtat tatttatgga
     2941 cagaacacca attatgtgct tccgatgttc atcatcatga cacttatgta cttcgtggta
     3001 tgttacgcgc tttctctgct ttccggttac ttggaaaaga gacggtacag ttattaatag
     3061 atgattcttt tagcggttgt ggcaaactaa agtcacaacc gcttttcctc tttccattcg
     3121 aaaaaaagag atttcgattg ttttaagcaa gtaagagcac ttgaaaattc acgctaaaaa
     3181 gtacacaata gggaggatag aatttattga aaaaaaggat gatacgattg acagataatc
     3241 gccctatcgg atttattgat tcaggtgtcg gcggcttgac tgtagtaaaa gaagccctga
     3301 aacaattacc gaatgaaaat attttatttg taggagacac agcacgctgc ccatatggcc
     3361 ctagacccgc ggaacaggta atccagtata cttgggaaat gacggattat ctggtggagc
     3421 aaggaatcaa gatgctggtg atcgcctgca ataccgcaac tgcggtggct ttagaagaaa
     3481 tcaaagctgc tctttctatt ccagtcatcg gtgtgatcct tcccggtact agagcggcag
     3541 taaaaaaaac acaaaataaa caagttggca ttatcggtac gattggtacg gtaaaaagtc
     3601 aagcttatga aaaagcactg aaagagaaag taccagaatt gactgtgaca agtcttgctt
     3661 gtcccaaatt tgtttcagtt gtcgaaagta atgaatacca ttcatcggtg gcgaaaaaaa
     3721 ttgtggcaga aacattagct cctttaacca ctaaaaaaat cgatacattg attttgggat
     3781 gcacccatta tccattatta cgccccatca ttcaaaatgt aatgggagaa aatgttcaac
     3841 tgatcgattc tggagcagaa acagtaggtg aagtatctat gctgttagat tatttcaatt
     3901 tgagcaattc accgcaaaat ggtcggacat tatgccagtt ttatacaact ggctctgcca
     3961 aacttttcga ggaaatagct gaagactggc ttggaatcgg acacttaaat gtagaacata
     4021 tcgaattggg aggaaaataa ggatgcgtca tgacgggaga aaagcccaag agcttcgcaa
     4081 actggagctt caaacaaatg ttttcaaata tccagaagga tcggtagtga tccgttttgg
     4141 tgatacaaca gttatttgtt ctgcaacaat agaaaattct gttccccctt ttttgagaga
     4201 aacaggaaca ggatgggtga cagcagaata tagtatgctg cctcgagcaa cgagtacgag
     4261 aaatcgtaga gaaagcagca aagggaaact ttctggtaga acaatggaaa tccagcgtct
     4321 tattggtcgt tccttgcgtg cagtagtaga cttggaaaaa ttaggagaac gtagtatcat
     4381 cgtagactgc gatgtgatcc aagcggatgg cggtactaga acagcaagta tcactggggc
     4441 atttgtggca ttgaaattag ccattgaaaa acttttaaga gaaaaagaat tgtcagaaga
     4501 tccaataaaa gaacatcttg ctgcagttag cgtaggaatc ttaccagatg ggacttgcgt
     4561 cacagatttg gattaccaag aagattccgc agcacttgta gacatgaatc ttgtaatgac
     4621 agaatctgga aaattcgtgg agattcaagg aactggagaa gaagcaacgt ttgatggtga
     4681 acagttgaat gaaatgctct tttttggaaa aaacgcgatt gaagatttga tcaaagagca
     4741 aaaacatgct ttgctaacag agtttgctca aaatgatgag cggatagaag aaacaaaaac
     4801 aatcatcatt gcgacaagaa atccaggaaa agcagaagag tttcgaaata tgttcaaaga
     4861 agcgggatat catgtaaaaa cattacttga ctatcctgaa ttgccagatg tggaagaaac
     4921 tggcagcaca tttgaagaaa atgcccgatt aaaagcagaa acgattgcac aattgcttga
     4981 tcagccagtc ttagcagatg attcaggttt gaaagtggat gctttaggcg gtatgccagg
     5041 aatctattct gcacgttttg caggggaaca aaaaagtgat gcggggaata acgccaaatt
     5101 attatatgaa ttgacagatg tgccggatga aaaaagaaca gcacaattcc attgtacgtt
     5161 agtattcgcg gcacccaaaa aagacagcct tgtagtagaa gcggaatggc ccggtcgagt
     5221 agcccgtata ccatctggcg aaaatggctt tgggtatgat cctttattta tcccagaagg
     5281 taagaaacaa acagcagcag aattatcgag cgaagaaaaa aacaagatca gtcatcgggc
     5341 tcaagcaatg aaaaaattga gtgccgagtg gaaacaatgg ctggaaggag agagatagat
     5401 gcggtattta gtagtaagtg ataatcatgg agacagagat atcctcgttg acttagtaga
     5461 tcgttatcgt tctgaagtgg atatcttttt ccattgtggt gattcggaat tagaagcttc
     5521 tgacccatta tgggatacat tccaagtggt tcaaggtaat tgtgattatg gaccaggatt
     5581 cgaacagaaa aaagtgatcc aaactggtca ggatacagta ttcatgactc acggtcattt
     5641 atcgaatgtc cgatttggtt tgacacagtt agcaatcgaa gctcgagctg caactgccga
     5701 tatggtgttt tttggtcata cccatcaaat aggctgtgag atggatcaga aaatcctgtt
     5761 tttgaatcct ggtagcattt ctcagcctag aggcccgatc caaatcccat catatgctct
     5821 tatcgaaagt ttagatgacc aaatttctgt tcaatactat aaccgtgccc atcaggcagt
     5881 tgaagagttg gccttttctt tttcgagaga gcgctgaata aaaaaactct aatttttaat
     5941 aaaaataagc tttattttat gaattttgca tccggtttcg gtaaaatagt agtgttgaat
     6001 gaaatagaaa tggaggccat cagatgattg gacctatagt aagagaaatg ctgctggaaa
     6061 atcaggaaac gtttttgatt cctgcagaaa atgtggcaaa tgttatgtat catcatccat
     6121 tgtcccatgg tttacttgtg ttatcgaagg tcggttattc caaaattcct gtattaggaa
     6181 aagatgatca ctttgtagga ctagtaagtt tatcagatgt ggtcaataaa atgattgact
     6241 tgcaaacaat cagcatggac ccgttagaag gactaacggt tgctgatgtg acagaaacag
     6301 atgttccgac aattgatgaa aattgggaac tagaagaagt actccatttg ttagtcgatt
     6361 cgcctttttt accggttgtc acagaagatc ggattttcaa aggaatcatt acaagaaaag
     6421 agctgcttaa agccgtaaac ttcatggtgc atgaattaga acgtcgtaat atattgtctc
     6481 cgaaaatcga agaattaaaa gaacaaatcg atatcgtgag ctaaagaacg agaaactaaa
     6541 agataatcaa aaaataagta gaaaaaacag cgtaccagtt ttgactttat cttgaaaaag
     6601 aggctgggac aaaagcgttt aactccaaga aataagaagg aattcacgaa aattgctttt
     6661 caaatttttg tgaatttcag cttatttccg aaggagttgc ttctgcttcc gccgtttata
     6721 cgtgtttaga gcgtgggaca aaagtgttct ttacttttgt cccacgctct ttttagtgag
     6781 attcgttgca tcgtttgtcc aaattatttt ccgacagcaa ttggactgtt cggaatcgtg
     6841 aaaccagaag ctgtcaattc tttgacatag gagctcaaga actcttcttt taaggcatat
     6901 tgttttccgt tcaatgcgta gcagaccgta cgaatcgcaa aattgctgtt tccaagatcc
     6961 actaatccaa agatagaagg ttcagtttgg agctcttctt ggtatttttc agctaatgta
     7021 cgatttactc gatcaatgat ctcatagatt ttgtcatatc cttcttcagg aacaatccga
     7081 atatctaaaa gtacttgcat atttgcacga gagagattgc tgatcgtggt gatgtttcga
     7141 ttcgggatga aatggacagt accatccgtt gctttcagct gaagggtacg gataccaaca
     7201 gatacgacgg ttccttcaat cgttaagttt gctagcttga tgtagtcccc aacatccatc
     7261 tgttgttcca tgataataaa aaatccggta ataacatcac tcatgaaccc ttgggcacca
     7321 agaccgattg ccacacctgc aattccggca ccagccaaaa gtgatccgat cggcacaccg
     7381 atagcagata aaattgcata gataaaaaag aaaccaaggg tataatgaaa aatagtattt
     7441 aaaagactat gtaaagtctt taagcggctc tcgtttagaa tggctttttt agaatactga
     7501 ttaaaagact tatcaatcag atattttccc acacggtgaa gaatccaaaa aaggacgatc
     7561 aaaaaaatca gatataaact tttttggata aaggcagcca aaatggcgtc ccaatcaatc
     7621 ccgtcccaaa accgctgaaa cgcatttact ttgctgacag cttgatctgt caatgtctcg
     7681 ccagaagagt ctgtcgtacc ggcagttgct aataatgaaa acataaaatc ctcttttcta
     7741 aaatttgatt ctttctgtat tgtaccaaga agttttcaaa aaaggaaata gcaaaaataa
     7801 ctataatgaa ggaaaaaagc tgaaaataaa atgacaaata atatggataa tgataaaatg
     7861 tggagaaaat aacgaaggtg gtaagtatat gcaaatcgat tggaacaatc taggtttttc
     7921 ttatatcaaa actccttggc gttttattgc caaatggaaa gacggacaat gggaagaagg
     7981 agaattgaca gaagataatt acctgacgat tcatgaaggc tctccagcgc ttcattatgg
     8041 tcagcaatgt tttgaaggtc taaaagctta tcgtcgaaaa gacggcaagg tcaatctctt
     8101 tcgacctgaa caaaatagtc gacggatgaa tcaaagtgct agacgattgc tgatgccaat
     8161 ggtccctgaa aagatgttcg ttgatgctgt caagcaagtg gtaaaagcaa atgaagattt
     8221 tgtgccgccc tatggaacag gcgctacatt atacttgcgc cctttactga ttggggtcgg
     8281 tgaaaacatt ggtgtccatc cagctccaga atatttattt gtcgtcttct gtacgccagt
     8341 aggagcgtat ttcaaaggtg gactaaaacc aacgaatttt attgtatctg attacgatcg
     8401 tgctgctcct aaggggactg gtgcagcaaa agtcggcgga aattacgcag cgagtttact
     8461 tccaggaaca gaagcaaaag aactggatta ctcagattgt gtttatcttg atccagccac
     8521 acatacgaaa atcgaagaag taggtgcagc aaatttcttt ggaattacaa aagacggaaa
     8581 attcatcact ccgcaatcgc cctctatctt accaagtatc acgaaatatt ccttgttgca
     8641 gttagctgaa gaacgactag gactgcaagc aattgaagga gatattttca ttgatgatct
     8701 agatcaattt gcagaagctg gtgcatgtgg tacagcggct gttatttctc caatcggtgg
     8761 gatttatcac aataacaatc ttcatgtatt ttatagtgaa acggaagtag gaccagtaac
     8821 aaaacggttg tatgatgaat tgacaggtat tcaatttgga gacatagaag caccagaagg
     8881 ctggattgaa gtcgtggaat aatttcaaag taatgagttg aaaaaaacgt aaaaagaaaa
     8941 aatcaggaag agactttaaa aagttttttc ctgattttta tatgttttac tttaaagaat
     9001 gcaacaaaag tttatgaaag atggacacct ttgtcaacgt agataatatc accgatgatt
     9061 ccgctggaaa gtgggctgat taagaaagca caagcattac cgacttcttc gatcgtcaca
     9121 ccttgctgat caggcgtacg agattcagat aactggatca gtttttgata gtctttaaca
     9181 cctgtgacag ctaatgtttt gattgcccca gctgagattc cattgacccg aattccttta
     9241 ggcgagaact cagctgccaa ataacggata gcagcttcaa gagaagcttt cgcaataccc
     9301 atcatgttat aattcgggat tgcgcgttct gaacctagat aagtcatgct aacgatcccg
     9361 ctgtttggtg caagaaacgg ttgtgcgtat tttgatacag caattaaaga gtagctgctg
     9421 atatcttgag ctaattgata gccgtctctg gaaatatcag acacatttcc ggacaattct
     9481 tctttgttgg cgtaagcaac tgcatggaca aggccgtgga tctccccagc atattcaccg
     9541 atggcattca ttgctttttc gatgctttcg tcgctagaaa catcacattc tacagtgaat
     9601 gcttcttcgc cgaccaattt gactaatgat tttttcattc gttcattttg gtaagtatag
     9661 ataatttctg ccccttgttt caacattgct tcagcacaac cccaagcaat gctttttttg
     9721 ttagcgacgc ccataatgac gatttttttt ccttctagaa atgacataat gactctcctt
     9781 tgtttctgat aaaaatttga tagtcgtaaa aaaatagaga ataatagcta aaataacttc
     9841 ttgatttct
//
LOCUS       DAZBSA010000099         9652 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000099 DAZBSA010000000
VERSION     DAZBSA010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.100"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..962
                     /locus_tag="ACHGMT_002330"
     CDS             156..962
                     /locus_tag="ACHGMT_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6836046.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            974..2194
                     /locus_tag="ACHGMT_002331"
     CDS             974..2194
                     /locus_tag="ACHGMT_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6836047.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            2184..3770
                     /locus_tag="ACHGMT_002332"
     CDS             2184..3770
                     /locus_tag="ACHGMT_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN6836048.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            3809..5947
                     /locus_tag="ACHGMT_002333"
     CDS             3809..5947
                     /locus_tag="ACHGMT_002333"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN6836049.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(6315..7298)
                     /locus_tag="ACHGMT_002334"
     CDS             complement(6315..7298)
                     /locus_tag="ACHGMT_002334"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFN6836050.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            7676..9067
                     /locus_tag="ACHGMT_002335"
     CDS             7676..9067
                     /locus_tag="ACHGMT_002335"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFN6836051.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            9083..>9652
                     /locus_tag="ACHGMT_002336"
     CDS             9083..>9652
                     /locus_tag="ACHGMT_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN6836052.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCE"
BASE COUNT         3229 a         1593 c         1897 g         2933 t
ORIGIN      
        1 ttgcaattgt ctagcatctt ctgtaaaata gaaacgttag agaaaatctt gtatcataga
       61 aaattaagaa agaaataaga acgcagctcg gtaccaaaaa tatggtaaaa tggcacttga
      121 ctgtaattca ctgaaaatcg cttggaggaa cacaaatgtt taaagatttg tatttattta
      181 tgaaggacat ttatcagaac agaaaattat tgctacagtt ttcgatcaat gacttcaaat
      241 caagatatgc aggatcgttt ttaggaatct tatgggcgtt tatcaatccg ctatttactg
      301 tattgattta ttggctggta tttggtgttg gactgaaagc acaaatgatc gatgcaaaat
      361 acccatttat cgtatattta gttaccggga tgattccctg gttcttcttc tcagatgcat
      421 ttgcatcaac tacggttgtt tttcgggaat atacttattt agtaaagaaa gtcgtattca
      481 atattcgtat cttgcccacg acgaaaatcc tatccaatct gtacacccac ttattcttca
      541 ttctgattgg catagctgtc gcgcttataa atggtatata tccttcagca atgtctttac
      601 agctgattta ttatttattt tgcatgtgtg ctttcttgac aggattgact tggctgacgg
      661 catcgatcca accattttta cctgatatca tgcaattaat tacgatcgtg ctgcagttga
      721 tcatgtggac attaccgata ttatggagcc ctaatcagtt tgattcaggt cttgtaagca
      781 tattgaaatt aaatccgctg tactatgttg ttcaaggtta tcgtgagtca tttttaagtg
      841 aagcttggtt ctgggagcat ggaacatata ctttgtattt ctgggttttc acgttagtaa
      901 tgttcgtaat cggctctgca gtgttccgta gattgaaacc acatttttca gatgtgctgt
      961 aaggagaata gctatgacag aatatgcaat tagactaaaa gatatcacaa aatcttataa
     1021 tatgtacgca aaaccttcgg atcgatttcg tgaagccctc aatcctttta agaaatccta
     1081 tcatgatgta ttttatgcgt tgaaaaacgt gaatgtagaa ataaaaaaag gtgagatgat
     1141 cggtttcgtg ggagaaaacg gatcgggaaa atctacaatg ttgaaaatca ttacgggagt
     1201 actgacacca acatccggca cgatggaaat cgaaggaaaa atctcagctt tgctagaact
     1261 aggatcagga ttcaatcctg agtattctgg ctatgaaaat atctatttaa atggaatggt
     1321 tctaggattt tcaagagaag aagtcgatga aatggtcgat gatatcattg aatttgctga
     1381 catcggagac catatttatc aacctgtaaa aacttattca agcgggatgt tcgttcgtct
     1441 agcttttgct gtagcaatca atgtgaatcc cgatattttg attgtggatg aagcattggc
     1501 agtaggcgat cttgaatttc aactaaaatg tatggaaaaa tttacagaaa tcaagaactc
     1561 gggaaagacg attttattcg tttctcatga tatcagttcg attcgtcgtt tttgtgatcg
     1621 ttcctattgg ttgaaaaacg gagaagtcgt cgaatatggc gatacgatgg aagtaacgac
     1681 gaattatgaa aactttttaa agaaaaaatc agttaaaaca gtggatcgga ataaaaacca
     1741 agtagaacgg ttcgccgctc cagatatcgt cgacgtgacg aaagcggaat tgttggataa
     1801 agacaaaaat ccggtggata tgctggaaca aggagaaaaa ctatacgtta aagtaactta
     1861 tgaagtaaaa gatgatacaa tcaaaaatcc ggttttagga attgcgctac gaacagtcga
     1921 caatttttat gtatgcggac ttaatactct actagatggt atcaagattc cttggaaaaa
     1981 aggagagaat atcttttatc tggagtatga aaagttaggt ttgttagctg gtgaatatta
     2041 ttttgacgtt gcggtctttg aagaaaatgc aacggttcct ttggtatata agacaaagta
     2101 catgaacttg tttgtcagcg gttcttatat cggagaaggc atcgtggtct tagaccataa
     2161 atgggaagaa gggactcata gcaatgaaat atgattttga aatggaccta gatgaacaaa
     2221 gcagtgtggg aaaaattgcc gcacagatca aaccaggaag taaagtttta gagtttggtc
     2281 ccggaaacgg ccgattaacg aaacatttaa tcggagcaaa acaatgtgaa gtgagcattg
     2341 ttgagttaga taaggaattg ttcgattttg tcagcgaatt tgctcaagat ggattttatg
     2401 gtgatatcga aagttttgag tgggcaaact attatgctgg acagacattt gattacgtct
     2461 tgtttgcgga tgttttagaa catttggtag atcctggaaa aacattgaag aaagtcagag
     2521 aatttttgaa tgaagaagga gagatcctga ttacattccc taacttggcg cataattctg
     2581 tcatgatcga tttgttcaat aaccaattgc cgtgggcttc ttatggtctc ctagatgaaa
     2641 cccataattc tttttatact catgatggct tccaaaaagt ttttgaaaaa gcgggattat
     2701 tcattaatat tgaagattat ttgtacttag ctgtaggtga tacagaatta aaatcaactt
     2761 atgaagaatt gccggaagct gtacgctatg atttcaaaat gcgaccattt ggtgaagtct
     2821 accaatactt tttctcattg atgaaacatc cagtagcaca aagttccatt gcagagccac
     2881 aaaattctaa ttatgtaaaa gtgttagaag taacccagca aacgaaacaa gatgaaacaa
     2941 tccaacaaat accattcaat aatttcacag gggaaaatga gacactaagt ttccccgtat
     3001 cagaaacaac agaaagaatg gtgtttcgct tcgcgcaaca acctagcttt atcgaattca
     3061 gcgcagaagc tgccggagaa aaactaacat ttatcgattc gaatgctgta gtcaaaacag
     3121 taaatgattg ttatttgttt gatgggaaag aattaccaga gttcgttcta acggatattt
     3181 ctgggaaaga agtgactatc cattgtcact atcgctttat cggggaattg acaccaacaa
     3241 tgaaagaatt gttggaaacg attcgtccca tggctgaagt cacaaagcag ctttctgaga
     3301 aaaatgacga attagaaaga gaaaatcatc atcttcttga ggaaaataca agactagacc
     3361 atacgttaaa aacaaccaca aatcgctatt gcacattgtt ggaaagtgat gaatggacaa
     3421 taaaacatag actaggacga cgtaagaaag aaacttcgaa aaaaatccaa gaaaaagaac
     3481 tttctatatg tattgacgag aaaatctggg atgcagaaac aaaaatatta aaaatcatcg
     3541 gatggggaat cgctaattcg gatcgccagc ccttgtccta taagttatca gctgaccaat
     3601 cgccattttt cgaagcaatt cctgtttcac gagaagaagt caatcaagca gaacagctcg
     3661 caaaaggaac agaagcggga tttgagttac gaatcctttg cgagcaagaa cgttcattct
     3721 tagtggaagc agtagctcaa aatggccaat catggatcat tgaaatgtaa ataatgatta
     3781 acttataaag ataaggagta aacgaatcgt gcaaaaaaaa gaaattgccg tatttatcga
     3841 tcaaatcact cgtgaacggg ccactggcga tttgcttatt gtgggctggg caattgatga
     3901 agtgaccaaa gaaatcccta cgataaaggt agaaaaagaa aatgtgattg ctgaagcaac
     3961 acatgtcgta agattggata tcaatcattt atataatctt gatgttaaga cccaaagcgg
     4021 ttttaaggta cgtttatcag ggaaaatgag aggaaaagcc atcttagact tccaaacagc
     4081 gagacatcaa aatggcatag ctgtgaaatt aaacggtaaa tacccttatg atgacggcat
     4141 tgaatcttca tgggaaagaa aaaaaagatt actgaaaaaa gggataaatt atgcaaggac
     4201 tcatggcgta aaaaaagcga tccgtcgggt caaattggaa ttgaagccag gatcgatcga
     4261 ctatgcagag tggatcagtc gtcatgagaa accagatttg aaagagcaaa ggaaagaagc
     4321 acaaggattt gcgtaccgtc cattgatttc catacttatg cctgtctata acgttgaaat
     4381 caaatggctc gaaaaatgca ttgattcggt actggatcaa acgtatgatc attgggaact
     4441 atgtatcagt gatgatgctt ctactgatcc agctatccga aagtgtctgg aatcctatca
     4501 agcaaaagat gatagaatca aagtcgtttt ccgaaaagaa aatggacaca tcagtctggc
     4561 aactaattct gcattggaaa tagcagaagg agaatttata gcactcttag ataatgatga
     4621 tgaattaccg ccatttgcat tatatgaagt ggcaaaagtg ctaaacgtac atccagaact
     4681 ggatgtgatc tacagtgacg aagataaaat cgatgcagat ggcaatcgct ttgatccgca
     4741 tttcaaagca gactggtcgc ctgatacatt gatgggaaat aattatatct ctcacttagg
     4801 cgtatatcga acaagcattg ttaaagaact tggcggtttc cgtaaaggct atgaaggttc
     4861 tcaagattat gatttggtcc ttcgtgtgac agaacaaatc cctgcagatc atatttacca
     4921 cattgaccga gtcttgtatc attggcggac gatcccagga tcaactgcaa gtaatggcga
     4981 agccaaaagt tatatctatg attccggagt gaaagcattg acagatgccc tgtctagaag
     5041 aaacatcaaa ggaagcgtac gtcctggcag aatttctgga ttttatgaga ttgcttatga
     5101 cgtcttgcaa gaggatttgg tcagtgtgat cattccaacg aagaatggct atgaagatct
     5161 aaaaacttgt gtggattcga tcatcgaaaa aacgtcttat tcaaattatg aaatcattat
     5221 tgcggataac ggaagtacag atccaaaaat gcaagaatta tttgcagaat acaagcatca
     5281 gctgaaagac cgctttatcg tagaattgat tgacattcca ttcaattatt ctcgaattaa
     5341 caatttagca gcagaaaaag caaacggcaa atacttttta ttcttgaata acgatacaga
     5401 agtgattgag ccagattgga tgacggtgat ggtttcttat gcacagtttg atcgaatcgg
     5461 ctgtgtagga gcaaaactgt tttatccaga tgatacaacg cagcatgcag gagtattatt
     5521 aggaatcggt ggtgttgcag ggcatgcatt gaataactat gatcgaacac attgtggtta
     5581 tttcggccga ttggtcattg acgtcaatta cttagcagtc actgcagctt gtatgatggt
     5641 caaggcagct gacttcaatg ctgttaacgg gtttgatgaa acattagaag tagcattcaa
     5701 cgatgttgac ttgtgcttga aagtttatga actgggtcgt tacaatgtct atgcacatca
     5761 agcagaattg taccatttcg aatctaaatc aagaggttat gaagatactc cagaaaaaca
     5821 aagacgtttt gctggtgaaa tcaaaaaaat gcaagataaa tggccagcat atattgcgca
     5881 tgatccattt tacaatgata atttaacaaa agaaggaatc ggcgactttt cgttgagacc
     5941 agattaaact ataataaaaa gctgttatgt tgtgattcgc tcgcacataa cggcttttta
     6001 tagttttttg tcaaaaaaag gcagaaggtg ccgactcaaa aaaataagtc gaggttggga
     6061 cagaagtgtt taactccgag aaataagaag aaattcacga aaattgcttt tcaaattttt
     6121 gtgaatttcg gcttatttaa gaagaagttg cttctgctcc caccgtttat tcgtttttaa
     6181 agaatgagac aaaagtgttt cttacttttg tcccacgctc ttattcatgc tcagagcgcc
     6241 aatagataca aaatcatagc caaagtttca cgaaccctag ctgtcaaaga atcgatattt
     6301 tattttaata taggctattt taaaataaat ggtttaaggt attcctctac aaaagccact
     6361 ctatcaatta tcatgggttt aaaataagaa gtgatggaga taaccagatt gttcttagcg
     6421 tcaatataaa gaacatttcc actattacca attgctgaat atatttcgtt ctcagaatct
     6481 aatacccacc ataaatagcc atatctcata ttttgtaagt cttctaaagc actggttcta
     6541 ggtttgaaca tctcgtttat ccacatgggt ggtacgattt gttttccttt atacatccct
     6601 ttatttaggc ataatacacc aatttttgcc agatcacttg ccgataaaca caaaccatat
     6661 cctgccgtcc cgatatttag tggatcacaa aaccagacat tggttttagg agacttatcc
     6721 attgtaaact gcttatgttc ttcagctgtt tcagcataat aattgacatg gtttggtatc
     6781 cctataggat caaatagata cttattggca aaatcaacag ttgttagctt acttgtctga
     6841 tacaacaagc cagttaatat gtgtaaacag acagtttgat agttgaattc gccggttata
     6901 ccttttcttc ctcccaataa atctagtgat gtttttgtcc aatcttcact tgaacagact
     6961 tttgtccatg gatcgccttt acatttgtag ggtgctttca ttgttagcaa gtgcttgatt
     7021 gtaatagcgt aaatagtttt ctccccgcgt ttcactttat actctggaaa ataatctaat
     7081 actttatcat cgatactttt gattaatcct tgctctaaag caatgccaat cagcaaagat
     7141 acaatacttt tcgttgctga catgatatga caagtatcgt cttttttata accattccat
     7201 tcatctgtat aaacttctac cccatcctta tatgcgacaa tttgacatat atttggttgt
     7261 cgaaaaccaa taaaatcatg caactcttgt ttattcataa gatattacct cactttcttc
     7321 tataagcata atgtcattaa aaatcgattt caattttttc gattcttcac gtaatacagg
     7381 tattcttatg gactagacag cgctttggga agatggattc tctgaaaaat ataaaggttc
     7441 actttatgag tggataaaaa aagcagcaaa tacttcgact tctttaattt accaacatta
     7501 gtgaaaatcg attcatctgg agatttgtaa tttagagtcc ttgtcttgat tagaggtgta
     7561 taaagctttt tattagacat ttttaaattc cattacaatt tgcttataaa ctaggttttt
     7621 aaaggtgact atggtaagat aatagagata ttattttgtg attaggagtg tatatatgaa
     7681 taaaaatgga gaatggagtg ctgcacgacg gatattcatt atattaatgg atgttattgt
     7741 atacaatgca gcaatatata gtagtttctt actgaagttt cgtggagaga tcccattacg
     7801 aaatttagaa actttccaac actcagcaat tattatttca gtgattttta ttgcgttgaa
     7861 cattttgttg ggtgcatatg ttttttataa tcgaatgatc agtgacatcg tgtttgtcac
     7921 agtcatcggt caggtattga tgacactagg gatcatggta gtgacttttg cgggccgctg
     7981 gtttgctttt ccacgtcttg ttatcttact ttcatttgtt ctcggtacgc ttttcttaag
     8041 tatctatcgt attctcgtat ataaacttta tttacgcgta agtcgagata aaaaggtcgt
     8101 cattattggg ttagaaaagg atgtcgcacc agcaatccaa aattttggtt caaagaaaaa
     8161 caacaaacat aaagtcgaag cagtcattct tgataactat tatgcaaaca tcaaaaatat
     8221 gattaaagac atcgatattg tctatcttgc ttctcatatc gaagaaactg aaaaaatcaa
     8281 aatctatcaa ttagttacta aaaatgaaaa aaaattgttt ttgaatacaa catttgaaaa
     8341 cttgacattg atcaatccaa atattatgaa tttcgaagat gaaagtatta ttgaagcatc
     8401 tggatttaga atccaacctg aatatgaact attcaaacgt ttgtttgata tcatcgtttc
     8461 acttgtttta ttagttattg cttcaccatt tatgttattc acagcaattt tagtcaaagt
     8521 gacatcaccg ggaccaatca tctataaaca agtgcggatc acaaaaaatc aaaaggaatt
     8581 ttcaatctat aaattccgct cgatgaccgc cacagccgaa gcaaaatctg gaccagtttt
     8641 agctaagagc aatgatgcac gagtaacgcc tgtaggtaaa tttattcgag ctgtccgctt
     8701 tgatgaatta ccacaaatct tcaatgtgct gaaaggcgat atgtcgatcg taggaccacg
     8761 tcctgaacgt cctttcttcg tcgatcagtt caatgcagaa aacccttatt actacttacg
     8821 gcataatgtt cgtgctggta tcactgggta tgcacaagtc tatggaaaat atgtctctga
     8881 ttacaacagt aaattacgtt ttgatttact ttatatcaaa aaatattcat tgatgcttga
     8941 tttaaagatc ttgctgcaaa ccatcaagat cctatttgat aaaatgtcct ctcaaggact
     9001 tgaagaagac gaaaatgtga tctcattggc agaaattgct tttccgaatg aaaaaatctt
     9061 tcgctagttg ggaggcgtaa aaatgaacaa tccgaaattc agcataatcg taccagtata
     9121 caatgcagaa gcaactattg gtcgaacgat tgatattcta aaagaattgg attatgaaga
     9181 gtacgaagtc gttttagtaa atgatggatc aacagatcaa acacaggaaa tcattcaaga
     9241 actgattgaa aatgatgaac gttttgaatt gattactacg accaatcaag ggcccggatt
     9301 agcaagaaac gaaggaataa gacagtcaaa aggagaatat ctcttattct tcgatgcaga
     9361 tgacacgcca gtaaaaacga tcttgtcaga ttatgatcat ttgctagaaa agagtccaaa
     9421 agcggatctt gttgttagtt cgtttgtttt tcgtacaatg gacgagggaa gaatcgtttc
     9481 cgaaaaagaa aacttagtta cagaatgtca ctatctaact cacgaagagt ttataaaaga
     9541 catgtatcag ttaatgaatg aacagttgat gtatgttgta tggaacaaat gctatcgtag
     9601 cgatattctg aaaaataacc aaattctatt taaaacttat aatagttgtg ag
//
LOCUS       DAZBSA010000100         9310 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000100 DAZBSA010000000
VERSION     DAZBSA010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.101"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(97..1464)
                     /gene="nhaC"
                     /locus_tag="ACHGMT_002337"
     CDS             complement(97..1464)
                     /gene="nhaC"
                     /locus_tag="ACHGMT_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFN6836053.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1573..2994)
                     /gene="tyrP"
                     /locus_tag="ACHGMT_002338"
     CDS             complement(1573..2994)
                     /gene="tyrP"
                     /locus_tag="ACHGMT_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFN6836054.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3237..5114)
                     /gene="tdc"
                     /locus_tag="ACHGMT_002339"
     CDS             complement(3237..5114)
                     /gene="tdc"
                     /locus_tag="ACHGMT_002339"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFN6836055.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5405..6661)
                     /gene="tyrS"
                     /locus_tag="ACHGMT_002340"
     CDS             complement(5405..6661)
                     /gene="tyrS"
                     /locus_tag="ACHGMT_002340"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFN6836056.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(6773..6978)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7241..8542
                     /locus_tag="ACHGMT_002341"
     CDS             7241..8542
                     /locus_tag="ACHGMT_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFN6836057.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8611..9210)
                     /locus_tag="ACHGMT_002342"
     CDS             complement(8611..9210)
                     /locus_tag="ACHGMT_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN6836058.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         2813 a         1925 c         1652 g         2920 t
ORIGIN      
        1 agctgaaatt cacaaaaatt taaaaagcaa ttttcttgag tttcttctta tttcttggag
       61 ttaaacgctt ctgtcccagc ctcttttttc cttccttcaa ttttttacac gtcgacgatt
      121 caataaaaaa ccgcctaata ttgtaaagac aggtgcaaga atcgagaaaa aggcgaatgg
      181 caagtaatgc aacgcgctaa ctttcatcgt tcccataatg aatgtgccgc tgactcccca
      241 cggtacgaga gaattgacgg tcgcacctga atccgctagt gttcgtgtca aataggtttt
      301 tgataagcca acgtcatcaa aagaacgctt gaatgtttct cctggaagaa tgatggacag
      361 atactgttct ccaacgatca gattgattcc gatacagctc aaggcagtaa acccgactaa
      421 acgagaaggt cggtccatct tttcttttaa ttcttgaacg atggtatcaa cgatcagata
      481 tttgatcagt aacccaccta atgccaatgc aagtaaaatc aaactagcag aacctaacat
      541 actcatgatc ccgcctcgag aaagcagttc atctagcttt ggatcaggtg tatttgcgac
      601 ataaccattc atcaagatgt ctgctgcttt tccgaccgtc aaacttttct catggataaa
      661 agaaagaacg agagcagctg tcgatcccgc tagtaatgta gggatagccg gaattttttt
      721 gattgccccc ccaaataaaa taactagtgg aatcaatgtc caaggagaga tccaaaagct
      781 cgctttcatg ttttggatca tttcattgat actagctaga tcggctgaat gactgttatg
      841 tcctaatatc gtataaaaaa tcaacgaaag gacgaatgct gggaaatccg tataacgcat
      901 accagcgata tgttcgaata gattaactcc tccaataccc gctgccaagt tggtcgtatc
      961 tgataaaggc gagcagttat tccctaaaaa ggcaccggaa acgactgcgc ctgtagtcat
     1021 tgcatcattg aatccaagta aatgaccgat ccccaaaaaa gcaattccaa tcgtcgaaat
     1081 cgttgtaaat gaactgccga cagtcactcc gacaattgca caaacaagaa atgctgtcgg
     1141 taaaaagaaa cgcacagaaa caagtttaaa tccataaacc ataatcgtag ggatggttcc
     1201 agataaaatc catgaagcaa ctaagacacc aatcaaaaga aaaatcagga ttggaatgat
     1261 ccctggagtg attcctttga tgatcccgtc atggatctca tcccatgtga actttttaaa
     1321 tctgccataa aacaacaaac acatgaaagc tgcaaggatc gggatatgtg gcgagagctg
     1381 gaatccgatg atcagtactc ctaggagtgc caacaacacg atcaatacag cgatactttc
     1441 ttttaaagaa atatttttat tcatcgtcga tctcctattt caaatgaaaa ggccctgcca
     1501 tgaaaaaact attcacttgg caggacctcc tactaaatca gcgacaaaac gtcggctgaa
     1561 agacttattt ttttattttt cttctaaact tttttctacc tcaatccccc aagaatcttt
     1621 cttgaagtga ttgatgatca atggaataac gaacatgacc actgtgatag ctgctactaa
     1681 taatgcatca ctcaatgtcg ctgtacctaa tgtagcaaat acgacaacga tgatagagaa
     1741 gatcagcatg cagctgacta cccaagctaa tccgtttccg tttttaccaa ttcggtaagg
     1801 acgttcgaat tctggatgtt tcttacgcat tttaagcaat gaaacagcaa tcaaaatgta
     1861 cacgatacaa taaataacag ttgtagcatt tgtaagtgtc aagaatacag cacttacgtc
     1921 atccattaca ccataaagca aagcaaataa tgaaataacg attgattgtg ttagaacgac
     1981 atttctagat acgccgtatt tgttttcttt atggaatccg aattttggtg gaaggaatcc
     2041 ttcacgagct acttggatga ttgtttttga tggtccagta acccaagctg ataattgtaa
     2101 aagcacacca ataaatacca tgaagctgaa gatatttccg atcacttctg gtaagcctag
     2161 gatttgacag tcaatcaaga taggctgcgt gatatttgct aattccattt ttccatctgg
     2221 tacgacattt gctacaagca tcgcattgat gacattcaat aaaactaatc cgatcaaagc
     2281 gataaatacg ccttttgtat aattttttgt tgcgtctttc aaacgaggca tgtaaacaga
     2341 actcatttcg atcccaacaa aaataaatgt gataccagct aagtatttca atgaatcaat
     2401 atgttccaag tcagggaaag cttttgacca tgaaaagtca cctagatagc cgtttgagac
     2461 tagtccgact ttgaaggctg ccaaaacacc taaaacgaac atgatgacta ctgggatata
     2521 tacccctagc catacaccga tgttcccgcc gacttttgcc atatcgaatt tcaagttcaa
     2581 gatcgtgatg atccagtaga taactaaaat acaacctaaa acgaaccagt gattattgcc
     2641 aagttctaca ttaccaaatg tgtttcctaa caatggtcct aaagtagaag cgaccattac
     2701 catacctggg aacatttgca cccataatag ccatgcaacg acaaatcccc atttgctgcc
     2761 cagtgctgtt tttacccata actgaggacc gccttcttga ggaagcatcg tggaaagctc
     2821 accggaaacc attgatatcg gaccggcaaa aaacaaaact gcaaaaattg aataaaaaat
     2881 caatgtccaa ccgactgcgg ctaacgtagg aatactccga accgttgcac acagtgccat
     2941 tgtcagaccg ataaaggtcc caagacccat tttacttact tcagatttac tcaccgttga
     3001 cactctcctt gattttttgg ataaaataac ggtgtctttg aagtagtttc agcaataaac
     3061 gaaacaattc agcgaatatt tgaggggatt ctttgagttt attgcttgaa gggtttcttc
     3121 aaagacaacc tcaaaacaaa gggatcgaag agcttaggtc tccccttgct cttcagatcc
     3181 aaacatgcgt cagaaacagt tagtattcaa gctgttttct aacatgatta ccttgcttat
     3241 tttgcttcgc ttgccaataa ttggtctgcg tagatttttt ccaatttttc ttgaagagct
     3301 gctttgattt tttcagcata ttcttcaaag tgttcttctt tgttcatgta tggtgtcatt
     3361 acgctggcac gcaatacagt tacttttccg gcacggttcc attcttcatc tgagaatcct
     3421 agttggttca caaattgtaa tgggctgttg ccatagtctg ggatagcaaa gtcagtatgt
     3481 gaagttaaga actcattacc gtaaatgctt cctttcacgt atgaagaata atcatatacg
     3541 tcatggttca atttattcat tgcaactaag tcatcgttgc ctttttcttt gaatacatag
     3601 tctaccatat tgaaatctgg ataagttaat ggatgaactt cgatttcttt gtctcctact
     3661 ttaaatgaca agtcattcaa gaagttgtag aaacgatgtg caccttcgat tgatgcaccc
     3721 attaatttac catatcctgt gacattcaaa ggtaagacat ggtgtgctgc ccaaacgctg
     3781 gctgctgttg cccctgcttt tgaaccttcc aagatataag ccccaagtaa tgctgggata
     3841 tctgcgcctt tttcaaatac atatgtcgca aagtaagaga taacgtctct catgcggata
     3901 tctttgatga cgataccacc ggcagaatat ggaacatagc ccattttatg tgggtcaatc
     3961 gttacagatt ctgcttcttc gattgcttta tatgcactgt gtacttcttc taagatataa
     4021 tctttgtttt ctgtaaagac attgtatttg taatgaacat cttttaaatc ttcaaatgga
     4081 atgaagttgt tgtcttcgtc taagaaaatc gcacggccgt atccgccata agcagcatct
     4141 acgtgtaagt agaagtaaat accgtctttt tctaatacgc gacgcaatgc aacgatttta
     4201 tcgataccgt cgattgctcc ttcttctgtt gatcctacaa caccgactac acctaggatt
     4261 ggtgtttttt cagctgctag accacgaacg attttttcaa gttcgttgat gtccatgcga
     4321 tagttgtggt caactggcac tggaatcact tggtctaaac cgataccgat gatgtcggca
     4381 gcttttagcc atgaatagtg tttagtttgt ggaaccaacc attttcctaa tttttctaaa
     4441 tgttttccgc tacgtgctga gtgagctttg atttcgtcga ttttttctgg gacgctgtct
     4501 aacagattca tgatttcttc tgttgacaag ttcattaatt cccaatcgct ttttccagca
     4561 actagttcag gtgtcacttc tttcattgca agcggcaatg atttgatgtt gcgggcatac
     4621 caaagtcctt ctaagtttgc taaagaacca tcagcaacga tgtggcccca gccatcttta
     4681 tagctcatta attttgcaaa ttccattcct acttcttctt ccatttggct tgttgcagga
     4741 gaagattcat aggcaacgtt attaccattc catagcatag caaaattgta cgctagtaaa
     4801 gatggcatca atgtttcaga gttcatatga ccccagtaac gtccggcatt atgccaagga
     4861 acggaatggg tacgcatccg cgctgaaatt tctgatagaa catcctttgt tttattcaca
     4921 gtgtgttcaa aactagcact gcttttttct tctggcgtaa taataggcat gtcttgaggc
     4981 atgtagtttt gacgccagcc taaatgttca tccaccaatt cattcaacaa agctttatag
     5041 atttggccgt tctcagcttt gtccccaatg aatagggcat ttaggtttag atctttcgac
     5101 aatgattcac tcatagtgaa accctcctaa aaaaatattt aaattttgtc gagccctaca
     5161 ttacacctgt tcttctattc tgtaaactaa gttttggcct gatttttaat catattttaa
     5221 tatgtatcaa ttatccgaat tgttcacact tttgaaataa ttaaaaaaaa cgagggaatt
     5281 tacaaatgta aactccctcg caaagattgt attaacgaca accagcgttc tattttagga
     5341 tgggtttcaa aattggaatg ttgtaatagt atttcccttt ttcaaggtaa aaacttacta
     5401 aattctaatc aattactttg gccaaaaagt aatttttctt accttttcga ataaccacaa
     5461 acttaccatc gaattcatcg gatggattta tgacaaaatt taaatcggta acgcggtcac
     5521 cgttaatact tatagctccg tttgaaacat cttcacgtgc ttgacgttta gatggttcaa
     5581 tttttgtgga aacaagcagt tccacgatat tttcaggtgt gcttgaaatt tcaacgttcg
     5641 gcattttacc gaatccttgc gcgatttctt ccgcatttag ttctttaata tttccagaga
     5701 acaatgcttg cgttattttt tgcgcttctt tcaagtcttc ttcactatgc acaaatcttg
     5761 taacttcttc tgccaaacgg cgttgtgctt cacgtttctc aggttctgtc tctacttttt
     5821 tcgctaagtc ttcgatttct tcttgagaca agaaagtaaa gaatttcaag tatttcacta
     5881 catcgcgatc atcttgattc aaccagaatt ggtagaactc aaatggtgaa gttttcttcg
     5941 gatcaagcca aacggcaccg cctgcagttt ttccgaattt cgttccgtca gccttcagca
     6001 tcaatgggat cgttaaaccg aacgcttctg cttctggtcc ttctaatttt cggattaggt
     6061 ctaatccagc tgtgatattt ccccattggt ctgctccacc gatttgcaat tgcacatcgt
     6121 atgttttatg cagatgcaaa aagtcgatag attgcaggat ttggtaagta aactctgtaa
     6181 atgagattcc tacttccaaa cggctggcaa cgatatcttt tgctaacatc gtgttgatgt
     6241 tgaagttttt accgtagtct ctcaaaaatt caagtaaaga gatttttgac aaccagtcgt
     6301 agttgttcac aaatgtgata tttgcatctt taccaaacaa ttttctcatt tgattggaaa
     6361 gtgcgtccac gttatgttgc acttgttcca tcgtttgcaa cacacgttct gttttgcggc
     6421 cgcttgggtc gccgatcgtt cctgttccgc cgccaatcag aatatatgga tgatggcctg
     6481 ccaattggaa tcttttcatc atcataaatg gaatcaaatg tccgatatgc atcgaatcac
     6541 cagttggatc aacgccgcaa taaagtgaaa tgctcttttc ttcaacgagc tcgcgaagtc
     6601 tttcttcgtt tgtttgctga ttaatggcgt cgcgccaagt taattcatca ataatattca
     6661 ttttactgac ctccgtgtag tttttttatg aaagcaggaa cctgctagca tatgtaatta
     6721 attatttctt caggatagga taggaaataa aaaaagtccc ttggcatata agccaaggga
     6781 cgaaatctcg cggtaccacc caatttgtat gtctagatat ccaacatacc tctcgaacat
     6841 ttaacgctgt cgcgtggcaa tcactgccaa agactcctga aatgtaattc gctttaaagc
     6901 atgtactggt ttgcagcgac caccagcttt ctgaaacatt ggctttaaca actacttcgt
     6961 ttcgatcttc gtcttgttat tattaatatg tgtctatcat agagcgcaac tttttctttg
     7021 tcaacagaaa aaggatattt tttttaaatt cctcattttt tcttttttga atccgctcta
     7081 attccgaaca ttttattttc aattattgtt aaaagaaata actttttctg tattttttct
     7141 gcaatttatc gagaagtcac tttactttgc ctcttattct ttcttcgtta caataaggaa
     7201 tgggacaagt aaaaaaatga aaaagcaagg gacgaggtat atggaaaaaa agcaaaaaat
     7261 tattttagga gccacttctg ctttaactat cgttatttta attctaagtt tgttttttgt
     7321 catccgatca tctacaaaaa aagaaccaat cattcctgaa aaaaaaataa cagaaaaagc
     7381 aaaagaaccg acagatgata agcaacaact agttactcaa gcaaaaaatc tggctcaaag
     7441 ctacgattat gaccaagcga ttgcgttatt ggaaaaagat agtaagaacg gatctactga
     7501 aacacaacag cttcttcaaa cactaaaaaa ggagaaagaa caactgatcg actggtctga
     7561 tcctactcag atctctcatg tctttttcca cagcttgatc gtggacccgc aaaaagcctt
     7621 ccactctcaa caggcacaag gatacaaaga ttatatggtg actgttgaag aattcaaccg
     7681 ttcgatcgaa caactttatc aaaatgattt tgttttagtt aatttgaatg atttgatcca
     7741 aaaagacgaa caaggaaatc tttcctttaa cgggcttgcg ctacctgaag gaaaaaaacc
     7801 attggttctt tcacaagatg atgtcagcta ttatgaatat atggataatt ccggttttcc
     7861 tagccgattg gtcgtatcaa ataacaatga aatcaaaaat gtctataaag agaaagataa
     7921 agaagaaatc ggtgactttg acatggttcc tctgatcgat gcctttgtca agaaacatcc
     7981 tgatttttct tatcgaggcg caaaaggtac cttggccctg acaggttaca acggagtttt
     8041 aggttatcga acatcaaaat ctcaatatgg agacaatgaa aaaacaaaaa aagagattaa
     8101 agaagctaaa aaagtggcgg aacagttgaa gaaagacggc tggagctttg cttcccatac
     8161 atggggtcac ttgaatatga ctcaagcttc actagaagat gtaaaaaaag acaatgaatt
     8221 gtggcagcaa gaagttgcac ctatcctagg aaaaacaaat attctgattt atccatttgg
     8281 tgcagacatc agcgactggc agccttattc agcagaaaat caaaaatttg cttacttgaa
     8341 gagtcaaggt ttcgatattt tctgtaatgt cgatgcttct acaccagctt ggggacaatt
     8401 aggtacggat ttctatcgaa atgctcggat caatatcgat ggcattcgtt ttcaatctga
     8461 tttagacgga aaaaatccta ttcttgatcc attcatcaat gtccatgatg tctatgacaa
     8521 agaagcacgc gaatctgctt gattttacgt catttatgaa ccaaaaaagc cgaactgaca
     8581 ttttatcgtc agtttcggct tttttatgct ttatttcttc ttcggtgcga ttcccttgat
     8641 caggacttct gtccattctg ctgtatatgg aagcagtttt gcgtatatct ccggcttaga
     8701 aattccttct accattgctt gaaaatagag gatcaagaac tcatccgaat atttcaaatc
     8761 cacttttcct tctttccgcc ctcgatggaa aagatcaagc atgatactga aactttcttg
     8821 cgtatacgca tccatcgcgt cagaaaattc ttttttttct ttttttgtat agtatgtcat
     8881 caaatcgata taaaattgtt tgttcactcg attgagataa cggattttgt tttcactcat
     8941 ggcaatcagt gtttcttcaa atggaagatt ttccgcaatc aacgctttcg cttcttgccc
     9001 taaatcattc ataaatgcaa aaaatacttc ataaatcagc ccttccttgc tgccaaaata
     9061 tttaaaaatc gtcgctttac ttacctgagc gttctcagct accttatcca ttgtcactcc
     9121 agcggttcct atgtctgtgt tcatcaagtc aaaagctgct tgtaaaatct ctttcttttt
     9181 ctcttgtgct ctcttttcaa aaccgttcat ttgtagagcc tcccgtaatt ttaaactttt
     9241 ataatatata tagttcaaat ttattctgag gtttattata actatctatt gagaaagtat
     9301 caagctagca
//
LOCUS       DAZBSA010000101         9130 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000101 DAZBSA010000000
VERSION     DAZBSA010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.102"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..561)
                     /locus_tag="ACHGMT_002343"
     CDS             complement(<1..561)
                     /locus_tag="ACHGMT_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6836059.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFI"
     gene            1078..2403
                     /locus_tag="ACHGMT_002344"
     CDS             1078..2403
                     /locus_tag="ACHGMT_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFN6836060.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            2396..2746
                     /locus_tag="ACHGMT_002345"
     CDS             2396..2746
                     /locus_tag="ACHGMT_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836061.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            2728..2913
                     /locus_tag="ACHGMT_002346"
     CDS             2728..2913
                     /locus_tag="ACHGMT_002346"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836062.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
     gene            complement(3817..4092)
                     /gene="prgO"
                     /locus_tag="ACHGMT_002347"
     CDS             complement(3817..4092)
                     /gene="prgO"
                     /locus_tag="ACHGMT_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFN6836063.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(4064..5017)
                     /locus_tag="ACHGMT_002348"
     CDS             complement(4064..5017)
                     /locus_tag="ACHGMT_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN6836064.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            5629..7122
                     /locus_tag="ACHGMT_002349"
     CDS             5629..7122
                     /locus_tag="ACHGMT_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFN6836065.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            7257..7553
                     /locus_tag="ACHGMT_002350"
     CDS             7257..7553
                     /locus_tag="ACHGMT_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFN6836066.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            7654..8505
                     /locus_tag="ACHGMT_002351"
     CDS             7654..8505
                     /locus_tag="ACHGMT_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836067.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            8526..8738
                     /locus_tag="ACHGMT_002352"
     CDS             8526..8738
                     /locus_tag="ACHGMT_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836068.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         3143 a         1424 c         1672 g         2891 t
ORIGIN      
        1 gataaattta tagatagtat ctttttgtgt ttttctttta aaaacacaac agagtgtgtt
       61 ataaggaacg ttataatttt catcagcctc attttttctc cattcaaaca aatgataaac
      121 tgcaaccaag ttgccatcaa tttcatggac agtgctctcg gtaacatcct ttaaagaact
      181 atcaagggct aatttatagt aactagagag aggaagtaaa attgtcgatt tactgagatg
      241 gttatcgtct atacgtgcaa tcatacctaa atcaactaag gttttattcc aggtgtttat
      301 agttttagtt gtcactccta gagactctgc tatggtattg accgaatgcc aggaatcccc
      361 gccttcattc tttgccttaa aacagtaata cagatataaa ttaatagctt tgttattcgg
      421 taaatatttt tcaagttctg ttggtagaat aaagtaagaa tccttaatgt tttttctgaa
      481 ctgtctccag tctctgtagt tttgtttata aagttgtgaa acggtttttc cacctttaga
      541 aagtcctgct atcttaacca atcttattgc tcctttcttg aatctgtcat gatcatacag
      601 tattttttac ttgtagtcca gcaccttaat atattatcct atgtaattaa tttcgtacta
      661 gatagtaatt attttttatt taattctcta gtcacttaca aataagttat ataaactacg
      721 attaataaat tattacaaaa actattaaga aaacttctac atacaactat tacaaaacta
      781 ttaagaaact gaatactcct attttttgtt tttaaggaaa gttttttctt ttttgaattt
      841 cttatttttt tctaatataa ctaagcaaaa ctggataata cgacacttat tttcaaattt
      901 tcacaagact aatagtaaat atccatttaa taaagtgata caatgtgttg attttacaaa
      961 tcaacagaag aagtagggaa ataacttacc tatgcttctt tttttgtaca tttaatcgat
     1021 tcttctttac aaaaacgaac gtatgtttgt ataatgtgga agaatggagt gttatttatg
     1081 atggattatt caaaagagcc agtaaatgat tactttttga tcgatatgaa aagcttttac
     1141 gcaagtgtcg aatgtataga aaggaattta gatccattaa cgactgaatt agtcgtcatg
     1201 agtcgagcgg ataatacagg atcagggttg attttagctt cttcaccagc tgcaaagaag
     1261 aagtacggta tttctaatgt cagtcgacca cgagatctac cgcaaccttt tcctaaaacg
     1321 ttacatgtcg ttccaccacg catgaatctc tatattaagc gaaatatgca ggtaaacaat
     1381 attttcagaa ggtatgtagc agacgaagat ttattgattt actcgatcga tgaatcgatc
     1441 ttaaaagtga ccagatcaat gaatcttttt acgactgaag gaacaagaag tcaacggaga
     1501 aagaagctgg ctcaaatgat ccaggagaat attaaagaag agctaggatt gcttgctaca
     1561 gtaggtgtcg gagataatcc cttattagct aaacttgctt tagacaatga agcaaaacat
     1621 aatgatggat tccttgctga atggacgtat caaaatgttc ctgataaagt atggaatatt
     1681 cctgaaatga cagacctttg gggtattgga tctcgaatga aaaaaagact caaccaaatt
     1741 ggtatcatga gtattaaaga cttagctaat tggaatccat acactattaa gagccattta
     1801 ggtgtgattg gcttacagct gtattttcat gcgaacggga tcgatcgaac agatatagcg
     1861 atcccaccag aaccgacaaa ggaaaaatca tatggtaatt cacaagtgtt accacgagat
     1921 tataccagga gaaatgaaat cgaactggtg gtaaaagaaa tggcggaaca agtagctatt
     1981 cgtatcaggc aacataactg taaaacaggt tgtgtccatt tatacattgg cacgtcgatc
     2041 ttagaaacga gaccaggttt ttcacaccag atgaagatcc ctataacaga taatacaaag
     2101 gaattgcaga attactgtct tttcttgttt gataagtatt atgaaggaca agaagtaagg
     2161 cacgttggaa taacctatag caagcttgtg tacactgatt cgcttcagct ggatttattt
     2221 agtgatccac aaaagcaaat tgacgaagaa aatttagata aaattattga caagattcgg
     2281 caaaaatatg gttttacgtc gattgtacat gcttcttcta tgttagaagg cgctcgtagt
     2341 attactagaa gtacattggt tggtggccat gcaggcggcg caggagggct taaaaatgat
     2401 tgattataat gatcgtggac gtttaaagtg ggcaggtttt tatttatctg atcatactga
     2461 aaaaattgat gccgacaatg aacaacgagc caatcaaaat ttggcaaaag aacaaatgac
     2521 gactgaagag gttgcggaag tattgaacgc tgcagtcata aaaaatagat cagtatgtat
     2581 tcaaaaagaa gaacgaaacg ctgaaggata ctatccagaa gatattgtta gcaagatctt
     2641 aggtagtgat gagctgggga tctacgttgg aaaaaacaaa gttcactacg acgaaatacg
     2701 tcatatcgaa ttttaccagg ataataaatg gggtcgatta gaatgaacat tatccagcaa
     2761 tatgagctaa agtatataac ctttgatcag ctatcagaag agatttgggg ttatggccag
     2821 cgtttgataa atgaggttgg tgttgaacgc ttctctttct atgtagaagc tgcagcagga
     2881 taccataaat acgaatacta tattggctat taaagtgcaa taaaattata tttcttttat
     2941 gttttacatt ttgtatcaat tctgataaaa ttacctatag gtaattaaaa aatataaaaa
     3001 aactagttac aaccttagga cgttgtaact agcttgaagc atactagagc taactaaaat
     3061 agcactagaa agcaatgtta aacccgtcca acaaacttga tttaacattg agaccagctt
     3121 attcagctaa atatagttga ataagcttct tttgttttaa aaaaacaaaa ggtgtactac
     3181 gaataacgca agtaaaagaa atactattac aaccagtagg ctacaagtaa gtattactaa
     3241 acattgcgta gttagaacct ttgctctatt gtctaaagcc acaaaattag agatcagctt
     3301 tatgatgcta agagcgagct gcatccatgc ggacataggt tcttttgaca ctattaattc
     3361 tcctttttag gataagggaa attgctagta gttttttcat tagcaccatc ccccttcatt
     3421 tgattgagtc tagtgctgct gtgatagttt gctgtttaat atgcttacat tcttattata
     3481 acataaatga aacaaacaca tggttagagg tcgtatcaat caaaatagtg tctcacaaac
     3541 gatttattac ttactcctgc tgactaacgg actgttcttc agtaactgta actatatgtt
     3601 tgttaaaacc accattacct gtagataaaa accaatatat ttactatact cactatatta
     3661 aatatattta gtatagtaaa aactagaaac aataaaattt gtgaatgttg tataccccca
     3721 aacaatacac atattttgaa ggattaggat taataaagct aaaaatatag taagtatact
     3781 aaatatattt actatatcta gtatacttac tatattttaa tcttttgatc gtctattata
     3841 tataatccgt tcctcgtcgt ttaaagtatc aacataggcg tccattgcaa gtttgactat
     3901 ttcatacata ggaatatctt ttgcataact aagatcccga atggttttta gtacgggagg
     3961 atctacttga actggttttc tttctttcgc agtgtacttt cgttccccta actcgacatt
     4021 aatatcatct ttaattgaaa tatcttcagc accaattttc ttcttatctt tgttcgttgg
     4081 taataatgcc atttagttta atctcctttc caagaggtag gatatgattg gccattgagt
     4141 cagcatattt aggtgtataa tcaaatcctt caatatcacc atgttgttca tagtattcaa
     4201 ttctttcttc taattctgta aagatgtctg caaaaatagc ccaccatctt cgatctacat
     4261 aatcatttaa ttggatacca ttctcaccat acatttctaa ccgatccgag cctttaacaa
     4321 tgcttttaaa aatgtttgat tcaccatact tatcaattgt tttttgatag tttgcgtgtt
     4381 gagaggaacg tcgcactgag aaaagaacag gcaaaattcc aatgatttga agatgagact
     4441 cttcaaaatt cgtgacaatc ggaactaaaa ctttattaat aaaatcttct gttccttcca
     4501 ttgcgtaccg ttttaattct tgcattgcaa taatataatc tgcagcagtt aagaatgcac
     4561 gaaccacatt atctgttgat ggtgggcagt cgataaagat ataatcgaaa ttttttctca
     4621 attcttctaa aggtttaatg aaggccaatg atctcttacg acgatcagga tatttttcgg
     4681 acaagtattc attaagttct tcagatccag gagagccagc cataatatat aaatgtggac
     4741 ttacttgagt aatacctgaa acccagtccc cttttttgat tgctgcagac atggatcgat
     4801 caaagctagt aatttgatac gttgatccaa tgttggaagt agtatttctt tgttggtcat
     4861 aatcaattag caacacgtct ttttcaaaaa gttgactaga aacaatcgct aataaatcat
     4921 tgtctgtagt cttaccaatg ccgccttttc tacctgtaag cattacaact tttcctagtt
     4981 tatctgatgt tttaaatgca gtccgtaagt tttccatagt tccctccaat ttcctatact
     5041 aaatatattt agtatacttc atatacttaa tatattcgat atatctaata tactccaaat
     5101 tgataagaat tcagacttta taaggaatat agtggatata ctaaatatat ttgctatatt
     5161 cagtatatca gatatattca aaatgtcaaa atatatttgt gcgttttgcg ttgacaattt
     5221 agagtgaaaa gaggtaaact taattctata gaaaccacag ataattcact atttacaaag
     5281 tagcaaaagc agtggtactc tttaaacaga aaaattacat aaaaaaaaga gacggaaata
     5341 attgatttta gcagaatcaa ttatttctga atcacatggt taggacatgt gttcacgctc
     5401 gtctcaagaa tatgtaagta gctaccaact acttatatta aaagttgatc ggataccttt
     5461 tccgattgcc tttattctac caaaaaaagg cgagcaactc aacagaagaa cgtttattta
     5521 gtgtacttaa attaaatatt cttaaacact gaagagagaa cacatactat ggctgagatt
     5581 aaagctaaac tgtgttctct ttttgtttga gaaaggacgt tacaatcgat gttgagcgaa
     5641 atttattcag ctattcttaa aaacggctta cgtacataca aatatcgaaa cagcaccatg
     5701 aaacctgcag gtcatattga ttccagtagg agtggtgcta tctttgcgta ccgtagcaag
     5761 caattaatga atgttgggcg tggtatggtc attacttcgg aagaagctat tttagagaac
     5821 gaaaagaaac taacgcattg gacacctaat acctatcgct ttggtactta tgcggatgat
     5881 tatcgaacag tggttaaagg tcattctgag aaaaacttaa gtcaaatcaa cacatttgta
     5941 gttgatatcg attcaaaaga gaaccatcaa ggcgaaatta ttttagcctg tttagatcaa
     6001 gtgggctaca tgccaacgct aattttagaa tcagatcatg gttatcaagt ttattttgta
     6061 ctaaagacac caggctatgt cacaaagaaa agcaatttca aggtgatcga tgtagcaaaa
     6121 aaaatttcaa aaacaattcg tctacagttg gctgaaaaat tgtccggagt ggatctagga
     6181 tgtaaccact tcggaattgc tcgattccca caagaaaata atattgtttt taaagaaatg
     6241 aactatcagt attcttttgc tgactggatt caatggtcga tgaagcttga atcagatcaa
     6301 aaaacaaaag aagatcgaga acgaaaacta attgtctttc ctgagaaaaa agaaattcgt
     6361 caagtagatg aaccgtggtt cgatatgtta cttcataaag caaacatcgt cggcggagaa
     6421 ggacgtcttg gtcgaaataa tgtgattttc acgctttctt tagcttatta ctcttcaggt
     6481 tatgcattgg atacttgcga atacaatatg ttcacattca acgaacggtt acaggaaccg
     6541 ctatctgaag cagaattagt aaagattgta cgtagtgctt attctggtaa ttatcaagca
     6601 gcaagcagag aattcattat tgaattgtgc cagcaatggg tttcaccaac cttaaaaaat
     6661 aatgatctgt tcattcaacg acgtggctgg tggaaattta aaaagccacg agatcaaaga
     6721 aaatatagtc ataaaaatga atggaaagaa gatctattgg cttatctaag cgaaaaaagt
     6781 cttgctagtg agccttattt gaacctgtca aaaaaagaat tggcttcaca actaacgatg
     6841 cccaagcgaa cacttgataa gatcttaaat gagttacgag aggagaataa agtcttctac
     6901 aaggtaacac gaggaagaaa tggcggaatg atcattgctt cagtcaaaac gttgttcgcg
     6961 agggttattc aactaagtaa agaaaagaga ctagattatt caacaaggat cacagaggta
     7021 tttggactca acgagagctt agtagaagag acatttagac aattatcaac ccccttgaac
     7081 cctcttaaag aagtgaatct ttttgaagta gatacaggtt gaaaaataaa aggtgcaaat
     7141 tgccttatac aatatatcta tgatacttct gtaggtgttg ctccctttgc ctagctacgt
     7201 gtaggtggta tacttataac tgcgtatgca gtcttatgta tcatggagga agttgtatgt
     7261 cgttaaaaaa ttatgtctat tcacatcctg ttaatgtgtt tatcattggt cgtttgggat
     7321 tatctgtgga agtgttctgt gagctttatg gttttaagca ggggactttg tcttcttggg
     7381 ttaccaggga aaaaacagtt gcttctttgc cgattgagtt tcttcatggg ttgtccttag
     7441 cttctgggga gactatggat caggtatatg attgtttgtg tgtgttagag caggagtata
     7501 ttgagtataa aatagctaac gagttaagaa aaaggaagaa gtatatccag tagtagtttt
     7561 tatttactta aaaaattggt tcattgagca acctatcgct gctaaagtag caaaggaaaa
     7621 cgttaaagga aagaatttag ggaagaaatc gaaatgaaat cggacgactg gattgaacac
     7681 acctggcttc ctctgaaaag aaatagcaaa cgaaagaaat tttgggtatt gcaattatgg
     7741 ccattaggaa aactttgtag ttacggggca cgacttattc ttgatttaac cgtttactcg
     7801 attggagtat ttggaacggc ttattacttg ataaatgatc ccaataggat tgcagcatca
     7861 gacattttta cagtgctaat gacgttgatt tttgtcatgc tattgtctat ttttactcaa
     7921 ttagtcatgc aatttaccag gcaaaaatta acagcaggaa aacttttcaa aagcctattc
     7981 gatttaattg ttgtaaatgc aattctagcg actttaataa aacaaattga acaatctgga
     8041 agaacaacaa aaggagtgaa ggatttttta ttgacgttta ttcactttgt aaatggacac
     8101 atgtatttgt ttgaagtggg tattattctc ttttttgtcg ggtatgtatc cacgaaactt
     8161 attaaacgtt caattgaaaa agaaattgaa attgacctga ttccgacact tcttggttat
     8221 gagtacaatg ttgattttgg aagatatgac gaattgatca atacggatat cgttcacacg
     8281 agtatccatg aatctactgt taagggcatc agatctattt taaaaaatgg ccaggaaatg
     8341 aaagcaatag atcgaagaaa agagatctac aatttttatg aagtcgaagc aagcctacga
     8401 tatggcatcg gaaaattgtg gttagatgga agtaatattc gtcaaaacaa accatacatt
     8461 ttttggaaca gtagtgattt agaacttcca atgaaagata aataattatg gagaatcgtt
     8521 tgaaaatgat tcctctatac aggattgtgc ttatagttgg catggagtta aaaaccttaa
     8581 agaaggagca gttattcaaa gtgaaagaac gtcataatca gttactatgt agtaaaacgg
     8641 gtacgatgaa cattgaatac cgaacagaat atttagaatt aacgaatcaa actgattttt
     8701 tgttgattcg aaagcaacaa gagcaagaaa aaaattaatg gctacttata gccaattgat
     8761 tgatgaatgg gaacaattca aggaatctaa aattccagaa cgtctctaaa agcctttatt
     8821 tgtaaagagc ataaggaaac gtgggaacac tttttaaaaa aagtgttcca ataggctgtg
     8881 cctattacgt ttctcaaaaa taaatgtggg aacaaaatat ttaaaaatag gatagtgaaa
     8941 ggatagtgaa ggatagtaaa aggagggtag taggatagca aaaaggatag gctccttttc
     9001 gctatccttt ttaaattgga ggaattaata tggaagtaag aataagaaat gtggatgaga
     9061 aaatcgtaag agaaattgat cgaagatgtg acgaacggtt ctgttgcaaa gttttaaatc
     9121 tactatcaaa
//
LOCUS       DAZBSA010000102         9125 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000102 DAZBSA010000000
VERSION     DAZBSA010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.103"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            7..912
                     /locus_tag="ACHGMT_002353"
     CDS             7..912
                     /locus_tag="ACHGMT_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFN6836069.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            1003..1680
                     /locus_tag="ACHGMT_002354"
     CDS             1003..1680
                     /locus_tag="ACHGMT_002354"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFN6836070.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            1677..1847
                     /locus_tag="ACHGMT_002355"
     CDS             1677..1847
                     /locus_tag="ACHGMT_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836071.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            1961..2857
                     /locus_tag="ACHGMT_002356"
     CDS             1961..2857
                     /locus_tag="ACHGMT_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFN6836072.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(2912..4282)
                     /locus_tag="ACHGMT_002357"
     CDS             complement(2912..4282)
                     /locus_tag="ACHGMT_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN6836073.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            4520..5266
                     /locus_tag="ACHGMT_002358"
     CDS             4520..5266
                     /locus_tag="ACHGMT_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFN6836074.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            5263..6102
                     /locus_tag="ACHGMT_002359"
     CDS             5263..6102
                     /locus_tag="ACHGMT_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFN6836075.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            6103..7052
                     /locus_tag="ACHGMT_002360"
                     /pseudo
     CDS             6103..7052
                     /locus_tag="ACHGMT_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
     gene            7193..7945
                     /locus_tag="ACHGMT_002361"
     CDS             7193..7945
                     /locus_tag="ACHGMT_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836076.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            7964..9115
                     /gene="queG"
                     /locus_tag="ACHGMT_002362"
     CDS             7964..9115
                     /gene="queG"
                     /locus_tag="ACHGMT_002362"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFN6836077.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         3162 a         1614 c         1913 g         2436 t
ORIGIN      
        1 atagacgtgg agcaatcgat ttttttaaaa aaggaacatc aacaagtaga aaaagaattg
       61 tattttgatt tttgtggttt ctctaaaacg atgccgtttc attcttttgg tcctgctatt
      121 cgcaatgatt acatcctgca tgtcgtgatg gaaggcaaag gatcttatca tgtgaaaaat
      181 caacaattcc aattgaaaaa aggtgatctg ttcttgattc gtccaggaga ctcgacgttt
      241 tacttatcag atggagaaga tccgtggatg tattgctggc tttcatttgg tgggagtgct
      301 gcagaagaaa tcattcggca gtcgcttttc agagaagacc aatacacgat ggtctcttct
      361 gacatacaaa aatatatcga cattatttta gaatgcatga aagatacgga agaaaccttg
      421 gaaaatgaat tacagttaac agcattaacc taccgttttt tatcagtcct tttaaaagat
      481 ggcggaaagg tcagcggtgg tgtacaaaag gttcattcct ctttagctgt tgaaacagtc
      541 aaatacatcg aggcacatta tgctgaaaaa ttgtctgttg aggaaatcgc ccagcattta
      601 gcggtcaatc ggagccattt gtcgcgagta ttcaaaaatc atatgggtac cagtatcaag
      661 gaatatttga tcggggtacg gatcaatcga ggggcgtttc ttctttctct gacggatgaa
      721 tccgtggaaa gtattgccta tcaagtaggg ttcaatagtc tagtcgtatt tagccgaatg
      781 ttcaaaaaga gtaccggaga aacggctacg aactatcgta aacgtatgaa aaacgaaaat
      841 tttaagaata ttgctttgga taccttgaaa aaacagttgg aaaaacaaac cgcagtttct
      901 tggacaacct gattagtctg gttaaaatga caagcatcgg caaaagaaac ttgtggcggt
      961 tatgctacac tgtaaagcga agactaaaaa ggaggaaaag ggatgcgtac acttatcatc
     1021 attagccatc caacgatcga agaatcttta aatcagcaat ttttcaaaga agcttctgct
     1081 ggtttaccag ctacttggca tcatttggaa tcaagttatc cagacggaaa aatagatata
     1141 gagactgaac tacagctctt ggcccagcat gatcggatca ttttccagtt tccgttttac
     1201 tggtatagtt ctcctgctca tttaaaaatt tggcaagata atgtcttgga acgagcagca
     1261 catgtattaa aagggaaaga actaggaatc gtattgacaa caggtgtggc ggaaaaagaa
     1321 taccaagctg gaggaaaaga agaatatacg atttccgaat ttttgaggcc gtatcagcga
     1381 atcgcaaata aattccatat gacctatctt ccgccattcg ttttagcaca atttatgtat
     1441 ttatcccagg aaaaacgttg ggagaagcta attgcttatc agcagtatct ctcattggaa
     1501 ggcaaaccat cgctgactca gcggattgac tggtttattc aaagagtaca ggaaaatcag
     1561 aaaatgcagg aagaagaatc tgaaaaacaa gcatatatcg tagaagcact gactgaggca
     1621 aaagagcaga tcgaagactt gacctttaca ctgcaagaaa tgaaaggaac ttcattatga
     1681 caaatgattg gctgatccaa caattagatg aattaagcga aaaatcagaa tatcaagacc
     1741 gtgcgctctt atatgaaata aagaaactga tggctgagat agacgaacga acaatgcagc
     1801 tgcaaggaga aatcgacgga caatcttgga atcatctcaa ctggtgaaat tttcttataa
     1861 ataacaaaga taaaaatgtg cacagcgaca attatttgct acaataagaa tatgaagcgt
     1921 ttgataacgc tattatacat aaaaattggt ggtgaaaaaa atgtatgatg aaaatgagtg
     1981 gggagaaaat gttcggatga cccgaagaag acagcaaaaa caggcagctc gtaagcggat
     2041 gatcatgatt ggggcagcag ttgccgtgtt tttgatcata gcaggaggag ttgcgggtct
     2101 atattttcat ggaaaaaatt cgtcagaagc gaaaagtacg accataacgg aaacaacgac
     2161 aaagaaaaca gagacgaaac aaacaagtga gtcatcaact aaaacaaaag agacacaaac
     2221 ttctgaagaa aaaacagcaa atggtttgcc agagcaactt caagcaatcg taaatgatta
     2281 ttcgaaaaag atcgaagaaa agacgcctgc attgattgaa gaataccaga cagaaattca
     2341 gggaaatcaa gagggaatag ctggtttatc agcagttgct aaccaaaaag cacgtgagct
     2401 tcaagcaatc tccgacgaag ggatcagaaa attacgtgca gcgtatcagg cagcagaaaa
     2461 taaagacggt gtagacttag atactttgat caatcaatta tcggctaatt atacgaatca
     2521 tgtcgctaag atttccgata tttatttgca gactagcgcc tctcttcaag cagagtcaac
     2581 aagcacacaa gacacaacat cttctgattc tgagacagca gagtcggtag aagcttctga
     2641 aatgcaagct cgaacaacac aagacacaac agattcctct gaaactaata ccaacagcgc
     2701 gacttcacag gctgcgacaa ccgtagtttt ggaaggagaa ggtccaaatc aaatagccga
     2761 acgaactggc gtaccagtag aaacgatctt gtcgctgaat gggatgagca tggagaatta
     2821 tttcctgact cccggtgaga cgctaaaatt gaattgataa aagcaataaa aaagaacgaa
     2881 gcttccacta aggtgaagct tcgttctttt tttattttcc ggttcttttt tcgcgtacaa
     2941 agtaatggat cgtcatgtaa agcaagaaaa cgagtccaat aatcaatgag attctcgttt
     3001 caggattgat aaacatgaaa atcaatacga tagctaaaaa agcaagtgtc acataattcg
     3061 tataaggaga tagtggcatt ttaaaaggat gatcatgcat tttatctgcg tgcttctttt
     3121 tgaagcgaat ctcgctgatc aaaatcacta cccaaggaac cataccaggc aaaatacttg
     3181 agctgtatac gaaaacgaaa atattttctg actggttgat gaacattggt agaagataat
     3241 ttaagatcag accaattaag attccacctg agattgctac gacaggataa aaaggcgcac
     3301 cgtgacgaga tagtttcaga aattttttag acatctttcc atttatggca agtgtataga
     3361 ccatacgact tgcgctgaaa atccctgaat tgcaacctga taatgccgct gtaatcacga
     3421 caaaattaat cagtcctgca gcaaatgtaa tgccgacttt agaaaaagtt tgtacaaaag
     3481 gtgaaccaat ctcactcaat tgattccacg gatagatact cacaatgata aaaatagcac
     3541 caatataaaa aatcaagatt cgaccgatag tggactggac agcttcaatg atcgtctttt
     3601 taggattctc tgcttcacca gcagtcattc cgatcagttc gattccttga taggaagcaa
     3661 caacgatcga taatgcaaag aaaaatcctt tgacgcctcc agtgaagaat ccaccatttt
     3721 tccaaagatt tgaaatccct actgcctgac catgattacc aaatccaaat aagattaccc
     3781 caagacctgc aatgatcatc aaaatgatcg ttatgacttt gatgatcgaa aaccaaaatt
     3841 ctaattcgcc aaacgccttg acagaaataa gatttgccag cataagaaac aagacgacaa
     3901 tgattccggg aataattgcc ggcaaatcag gccaccaata attcatataa gatcctacag
     3961 caatcacttc acttattcct acgacaatat attgaaaaat attactccaa gcggtcaagt
     4021 agccagccac aggatggatg tactcgctcg cgtaatttgc aaaagaacca gtcgaaggat
     4081 caacgtaaag catttcgcct aaagcacgca taatcaaata caaaaagaat cctgcaatcg
     4141 cataggctcc catcactgag ggccctgtcc aacgtatggt tgaggaggca cccataaaca
     4201 gaccgacacc gatcgttcca cctagtgcga tcatttggat atgacgggca ctaagacttc
     4261 gagttaactt ttcttcttcc atacaaaaac tcctttcgtt gttgagtcaa caagaaaaaa
     4321 taatcaaacg tttcaacaca tgtttttcat tattaggtta gccttattga actcttgatg
     4381 tgattctaca gaaaattttc ataaaatgcg aggaaaattc gaaaaaaact ttttcatcat
     4441 tctacttgaa aatgctttcc attttagtta aactatattt aaataaaaag ttaggtaaac
     4501 ctaaaaagga gtgtaagatt tggaacgtac ggccattgta attcaagacc tttcagccgc
     4561 ttatcaaggg aaaactgttt tgaaaactat caacttgacg ataaatccac aaaaaattac
     4621 cggtattatc ggaccaaacg gtgcaggaaa atcaacattt atgaaatctt tgctagagtt
     4681 gataccagct actactggta aagtcacctt ttctggacag tcggtaaact cagtccgtaa
     4741 aaaaatagcc tacgtggaac agcgaagtga attggatctt tcctttccaa ttgatgtatt
     4801 aggcgttgta cttttaggaa catatccatc tttacgaatt ggacaaagac ctgggaaaaa
     4861 agaaaaagaa cgtgcaagac aagctttgaa aaaagtaggg atggaagaat atgccaaaag
     4921 acagatcagc gaactatcgg gtggacagct tcagagagtt tttattgcaa gagctctagc
     4981 ccaaggagca gaatggatct ttttagatga accattcgta gggattgatg cgttaagtga
     5041 acgaaagatc tttgacatct tgcaggaatt gaagaattca ggaaaaacga ttttgatcgt
     5101 ccatcatgat cttcataaag tagaagaata tttcgatgag gttattcttt taaataaaca
     5161 gctgatcgct tccggtccag tacaagaagt ctttacatcg aagaaccttc aattggccta
     5221 tggtgagatc atcagacatt tagtaaaagg aggagagaaa aaatgatcca atcctttatt
     5281 gatggattaa tgaattatca attcctgcaa tacgctttgg tcacttccat gcttgtagga
     5341 cttgcttctg gagtcatcgg ttcttttatt attttacgag ggatgtcttt gatgggagac
     5401 gctatctctc acgcagttct tccaggtgta gccatttctt atatgtttgg ttttagttac
     5461 atctttggag ccactgcttt tgggatgcta acagcagcag tgattggttt cgtgactcag
     5521 cggagccgat tgaaaaatga tacagcgatt ggaattgtct ttagctcctt ttttgcatta
     5581 gggattatct tgatttcata tgcccagagt gcgacagatt tgtatcatat tttatttggg
     5641 aatgtcttag ctgttaggga aagcgatttg ttgctgacag cactcgttag tggaatcgtc
     5701 ttgatatttg tctttttctt ttataaagaa ttgaagatca catcattcga tccgacaatg
     5761 gcaaaagctt atgggctgaa cacatcgctg atccattatc ttttgatgtt ctttttgaca
     5821 ttagtggctg tagtcagttt acagacagta ggaacaatct tggtgattgc catgttgatc
     5881 acaccagccg ccacggctta cttgctaacg aaccatttac tgaaaatgat cattacagct
     5941 gcaggaatcg gtatgctaag tgcagttgtc ggtgtgtttt tcagttatag ttacaattgg
     6001 ccatcaggag ctacgatcgt gttagcatgt accgcatttt ttatccttgc ttttttattt
     6061 tctccaacaa aaggaatttt atttcagaaa ggaagcagat agatgaaaac aaaaaaatca
     6121 ctctttttaa tcttggcagt tagtttcttg gtattagccg gttgcggaaa gcaggcttct
     6181 gaccaagcgg atgaaggaag taaagaaaag ttgtcagtcg tggctaccaa ttcgatcttg
     6241 gcggacatgg caaaagaggt cggaacagat caaatagata tccacagtat cgtaccagtc
     6301 ggaacagatc cgcatgaata tgaagtacta ccagaagaca tcaaaaaggc aagcgatgca
     6361 gatgttattt tatacaacgg tttgaatctt gaaacaggta acagctggtt cgataacttg
     6421 atggaaacgg ctaaaaaaga agaaggaaaa gattattttg cagttagcaa aaatgtagaa
     6481 cctctttatt taactagcgg tgaagaacat acaaaagcag atccccatgc ttggctagac
     6541 ctatctaacg gaataaaata tgtggaggaa atcgcacgta tattctctga aaaagatgca
     6601 gaaaatgcga cactctataa aaaaatgcag aagcatatgt ggaaaaacta aaagaattag
     6661 atacacaagc gaaggaaagt tttgcttcta tcgaagagaa caaaaaatta ttagtaacaa
     6721 gtgaaggggc attcaagtat ttttcacgag catatgattt gccagcagct tatatatggg
     6781 agatcaatac agaaagtcaa ggtacgcctg atcaaatgaa agcgatcatt gatcagataa
     6841 gagcaaaaga agtaccggtt ttattcgtgg aaaccagtgt agactcaaga agcatggaac
     6901 gggtagcaaa agaaacgggt ttgaaaatct atgataaact gttcactgat tccatagcaa
     6961 aagaaggaga acaaggagat tcttactatc agatgatgaa atggaatatt gaaacgatcc
     7021 atgaaggttt gtcacaaaca aaagaaagct gaaaagtgca aggtctagct tgaggagcag
     7081 accttgcatt ttttataaga gatatgaaaa aaaattatgc tgtttttcgt cgtacagata
     7141 ccttttttga agaaattccg ttatagttag agtaaggaag gaaggtgtgg ctatgaattt
     7201 tacacgtaaa gataaatata cgattaaaga agaagtcttt cgattgatca gccagcagga
     7261 aaataccgac aaggatcaag accttcaatt tcttgaagca gaattagcta aattattcat
     7321 agcaaatcaa aatgagacaa cagaaccaga tgaagagctg tacataggca aatggttgtt
     7381 tgatgaattt cagcctttag atgaaaatac cggtatcggt tattttgtgt tcctttctcc
     7441 agtcggcttc acacctggcg tattagttaa acaaaacgac gaaataaccg tcaaagcatg
     7501 gcctgcatta ttttcagtca tcaaagcaga aggagtaatc aaaaagatcc agtcggccta
     7561 ttataaaagt caatttattg ccaacagcaa attaatgaat atgttttata atcgtctagt
     7621 agaaattgct aggcctggga aaatcccgca aacattcacg tacacacagc aaaaaaatct
     7681 ctatgaagaa gtattgtatt cccgcgatct gtctgagttt tcgccatggg aagaggtaag
     7741 gctccatgaa ctgttaagta caaaaccagt ttcattgaat ttttttaatg cattagataa
     7801 caaaagaaaa ttaatttcaa caaattattg ggaagtattt gctgcaagaa aaaaatggat
     7861 ggaagaaaag aagattgccg atcagcagat ccagtttgtc cagctgaaaa aagaaaaact
     7921 atattatggg ttatacagcc gttaacagca agcgaggaag accatgacat taaaagaaga
     7981 aatcatacaa gaaagcaaac gattaggaat tgacaaaatc gggttcacca cggcggaacc
     8041 ttttgattca ttaaaagaat cgttggaaga acaaaaagca gctggccata cttctggctt
     8101 tgaacatcca aatatcgatg agcgtcttta tccagaaatg acatttgatc agccgaaatc
     8161 gatcatttcc attgcacttg cgtatcctac taggatccat gaagaagtgc ctagggatga
     8221 aaaacgcggg cagtttgctc gtgcttcttg ggggatcgat taccatgata tactcagaga
     8281 ccgtttgaac cgattgatcg aattcatcca aacaagagca gaaaaatggc aaaaagaaga
     8341 ggaatggcgt ctagctccgc aagtagatac tggagaactg atcgatgtcg ctgtagcaca
     8401 gcgagctggt ttaggattta ttggacgaaa cggattgttg atcactgaag agttcggttc
     8461 atttgtctat cttggtgaga tcgttactaa tattgtattt gagcctgata aaccaggaga
     8521 atttggttgc ggtgactgta tgcgctgcgt gactgcttgt ccgacaaaag cattgcttgg
     8581 tgacggaaga atgaatgccc agcgatgttt gtcttatcaa actcagacaa aagggatgat
     8641 gccggaagaa tatcgtaaaa aaatgcgaaa tgtcatttat ggatgtgata tctgccagtt
     8701 agtctgccct tacaatcaag gaaaagattt tcattttcat gaagagatgg aaccaaaagt
     8761 agacgaaata tatccaaaat taaagccaat gctttcgatg tctaataaag acttcaaaca
     8821 gcaatttggg catttagctg gttcatggcg tgggaagaaa cctttgcaga gaaacgcatt
     8881 gattgcttta gctaatctag gtgatcgaac ggcattgcct gagatttcag aatgtttaac
     8941 agatgtccgc cctgtcatca gaggaacagc agcttgggct attggacgtc taggaacaaa
     9001 agaaccggaa aaatggcttg atgtattaga tgaaatatta gctaaagaaa cagaagaagt
     9061 cgttataaac gaattggaac atgcccagaa attattgcgg agaaagttaa aataaaaaaa
     9121 tagtt
//
LOCUS       DAZBSA010000103         9018 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000103 DAZBSA010000000
VERSION     DAZBSA010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.104"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1101
                     /locus_tag="ACHGMT_002363"
     CDS             <1..1101
                     /locus_tag="ACHGMT_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFN6836078.1"
                     /translation="KKETIVRFEQYNHMPLVTRLKKQRYTCKNCRTHWTAQSYFVRLS
                     HSIADHVRYKIASLLTEKVSLSFIAKSCQVSLTTVTRTLKEFKSYLPKPSKKILPRVL
                     MVDEFRSHASIEDKMSFICADGETGNLIAVLPTRKLPRLTSYFLGCTNPEEVEFLVTD
                     MNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQALNELRIREMNELRKAGQKSQAEKLKK
                     NWRFLLKNRASINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSAPLKEAYPFFHELV
                     EAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITSAMIYPYSNGKIEAKNT
                     HIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
     gene            complement(1280..1615)
                     /locus_tag="ACHGMT_002364"
     CDS             complement(1280..1615)
                     /locus_tag="ACHGMT_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFN6836079.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(1599..1862)
                     /locus_tag="ACHGMT_002365"
     CDS             complement(1599..1862)
                     /locus_tag="ACHGMT_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFN6836080.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2322..3125)
                     /locus_tag="ACHGMT_002366"
     CDS             complement(2322..3125)
                     /locus_tag="ACHGMT_002366"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFN6836081.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3103..4227)
                     /locus_tag="ACHGMT_002367"
     CDS             complement(3103..4227)
                     /locus_tag="ACHGMT_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6836082.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4243..5058)
                     /locus_tag="ACHGMT_002368"
     CDS             complement(4243..5058)
                     /locus_tag="ACHGMT_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6836083.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5048..5920)
                     /locus_tag="ACHGMT_002369"
     CDS             complement(5048..5920)
                     /locus_tag="ACHGMT_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6836084.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6005..7294)
                     /locus_tag="ACHGMT_002370"
     CDS             complement(6005..7294)
                     /locus_tag="ACHGMT_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN6836085.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7438..7950)
                     /locus_tag="ACHGMT_002371"
     CDS             complement(7438..7950)
                     /locus_tag="ACHGMT_002371"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836086.1"
                     /translation="MTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQLSWAAIWG
                     KHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAREVGQQLG
                     LTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGVKRFGDGI
                     HDHMGALFLA"
     gene            complement(8017..8160)
                     /locus_tag="ACHGMT_002372"
     CDS             complement(8017..8160)
                     /locus_tag="ACHGMT_002372"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFN6836087.1"
                     /translation="MITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLILA
                     HDR"
     gene            complement(8643..>9018)
                     /locus_tag="ACHGMT_002373"
     CDS             complement(8643..>9018)
                     /locus_tag="ACHGMT_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN6836088.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTVWLSVRTAFFVG"
BASE COUNT         2778 a         1933 c         1427 g         2880 t
ORIGIN      
        1 aagaaggaaa cgattgtccg ttttgaacaa tacaatcata tgcctttggt tacgcgccta
       61 aaaaagcaac gctatacctg taaaaactgt cgtacccatt ggacggctca aagttatttt
      121 gtccgactca gccattcaat cgcagatcat gttcgatata aaattgcttc tttactgact
      181 gaaaaagtat ctttgtcttt tattgcgaaa agttgtcagg tatctttgac taccgttact
      241 cgtacattga aagagtttaa aagctattta ccaaagcctt ctaagaagat tctccccaga
      301 gtattgatgg ttgatgaatt tcgttcgcac gcttccatag aagataaaat gagctttatt
      361 tgcgcagatg gtgaaacagg aaacttaatt gctgtcttgc ctacgcgtaa attacctcga
      421 ttaacaagct atttcttagg ctgcaccaat ccagaagaag tagaattctt agtcacagac
      481 atgaacgccg cttacttcca gcttaccaaa cgtgttctgc caaatgcgaa agtagtgatt
      541 gatcggtttc atattgtcaa acacatgaat caggcactca atgagttgcg tatccgtgaa
      601 atgaatgagc ttcgtaaagc aggacagaaa agtcaggcag aaaaactgaa aaagaactgg
      661 cgctttctgc taaaaaatcg tgcaagcatc aaccattatg agtacaaaac atggaaaagt
      721 ttccgagcac caaaataccc atttctcact gaagcaatga tgattgatcg attgcttgag
      781 ttttctgcgc ctctaaagga ggcgtatccc ttttttcatg aattagtcga agcctttcga
      841 gacaaagacc ctgacttatt tttctcctta ttggcagaac ttcccgaaac gttagatgac
      901 ggctttcggg agaagcttca aaaccttctg acctatgaag aaggtatcac cagcgcaatg
      961 atctatcctt attccaatgg aaaaattgaa gcgaagaata cccacataaa gacaatgaaa
     1021 cgagtatcct acggatttaa atcattcgag aacatgagaa ttagaatctt tttgatcaat
     1081 caattaatca atgtaagata acaaaaaatc tgaaccagaa tgtggttcat tctgacccgg
     1141 atttgatttt gcacaactca tcagtcctta ttgacaaaga gccaaaaatc tgagccagaa
     1201 tgaggttcat tctgacccag atttgatttt gtacaaccca tcagtcctta ttgacaaaga
     1261 gcccttcttt ttagtattat tataattctt ccatgcgttc ttccagtcgc tgtaaatcgc
     1321 ttggacttaa ttccccaatt tttttccatt tgaccttttt gttgtttttg ggtaattcgt
     1381 ttagagttcc tacgtcaatc caagattctt tttttagtcc tgcctgtttc caatccctta
     1441 tttcaacgta tttcagcttt attttatcgg atttagtatg atattttgaa gtaattgaca
     1501 gaaacgaaat tgtgtccagt ccatttttta gaatatagac aggccggcgt ttaccgccct
     1561 ctgcagctgt gaaagcaacg tagacggttg ctatatcatt agtattcatc ttcattgaac
     1621 cattcttcaa ttttttcgtc agtgtctagt ttttggacag gcaatttttc cgctaatctt
     1681 tgaatattat ggattcgttt acttttttcc gacaattcaa tagaaaatgg gatttttcct
     1741 tgctcaacaa tttgcttata aaaaacggtg acggctgttt tggggctcat gcctagttca
     1801 tcaataatgc tttctgcttc atttttcaat ttttcgtcca tagaaatttg taacttagcc
     1861 attttctcca cctctttctg acaaacacat tatatcacgg aaagagttca aatggaatta
     1921 atacaactac ttttgaactc cttgaaatgc agtgtattta tctaaatata aggaaaggat
     1981 atagtgctaa atggccttga tagatagaaa agtttcaaaa tccgccacag cgataattga
     2041 agcgaacact caacgacaca cagaacagga aaagactcct aaaagaacca aattaactag
     2101 gacgtgtctg aaaactccac aaagatgtgg tatgctctgg taaaaaacga atggcacaac
     2161 cacgaagacc gatatggttc ttggaaaact ttctccaccc tttttgtaat tggagggata
     2221 gtggtttatt gatagcgatt ttccaagttc ttcaagtgga accagacatt gagcaccacg
     2281 gctaaaaagt gaatgaagtc tttattttta atcaataatt tttaatgatt accagtaatt
     2341 ggttttttca tgcgccctat ttctatttga tgtggagtta aataatgctg ataagtaaaa
     2401 atttttccgt caattaaact gccactagtg aatcctgttc tattggtaaa aagagaatag
     2461 tctgggtatt catctacacc aaatgataaa gattcagcca taaactgttt tttaccagca
     2521 aattcagcac tgctaccttg atggatatgt cctacaaaaa tacctaaaat atcactttcg
     2581 gcaatcaatt cagcaaaacc ttccggaact tcagttacaa tagatggatc ttcccacgac
     2641 aatggatgat gaaccaataa aatcgttccc ttttcagcag gtgtacccaa ccactcttgt
     2701 aattgaatca tctgtcctat cgatattttt ccttcgtgct gggtatcttg agcagaatcc
     2761 aaggaaataa ttcgtaaccc attttgattc gtacaatgga tatactctcc cgtttcattg
     2821 ctttcaacac tcattccttc aaagaaaaca ctgcgacgat catgattccc acgacaaaaa
     2881 tagtaaggtg tggttgggaa atattgatcc aacattttcc gtaagacttg ataatcagaa
     2941 atcccccctt catgtactaa atcacctgtt aatagaataa aatcgtacac tgttaacgag
     3001 agttcttgta acaatttttc caatgccaac tgtggatttt taattgttgg aaatagttca
     3061 gcagtaaaca agttagtttg atagttttct ctaaaatgga tatcagagag atgcaaaaag
     3121 cgcatggcta ttccctcttt cttcaataaa ataatcagga tatcctaaat tttcttgcgt
     3181 ctgataatca aaaaaatgca ttctttcagg ttctatgcgg agattgactg catcaccaat
     3241 ctgccaagta ggtttttcat taaggacaat catttctata ccatcgactt taacggtgta
     3301 cgcataactt tgacctaata gctcacgata cttcacttga ccgtgtaaat cgcttgtttc
     3361 ttttgaaaag gttacgtgtt ctggtcttat gcctaaatac aatgagccac taacctgaag
     3421 aagttgtttt tgccaagctg gattcaatgt taaaaattga ctggctacct ttaatgttcc
     3481 ttgatcataa gtaactggca taatattcat gcctggcgaa ccaataaaat ttgcagtaaa
     3541 agtattttta ggtttcgtat aaacattttt cggcgtatca atcatctgaa ttctaccttc
     3601 attcaataaa acaatccggt cagctaacgt catcgcttca atttgatcat gagtgacgta
     3661 gacaaaagtt tgtttataac gttgatgaat ttttaccaat tcttttcgtg catccaatcg
     3721 taaacgcaca tccaaatttg ataacggttc atctaataaa aaataattac tccgtttaac
     3781 caccgcacga gccaatgcta cacgctgccg ttgccctcct gacaaatctt taggaagtcg
     3841 atcttggtat ggcgttaaac ctaacatctt caaagcttct tccaaacggt gctgaatgtc
     3901 agctgtctta attttattcg cccttaatgc aaaaataata ttctccttta cagtcatgtg
     3961 aggatataaa gcataatttt gaaaaaccat tgcgatctct cgttctccac aaggtagctt
     4021 atttgctagt tgtgtaccta aaaaaagctc tccactcgta attttttcca aaccagcaat
     4081 cattctcagc aaagtagatt ttcctgaacc agatggtccc agtaacacaa ttcgttctcc
     4141 agcggccaca gctaaatcaa tttcttttaa aatggtatgt tctccatatt tttttccgat
     4201 atttcttaat tcaagcgttt ctcccatgat ttgtcctcca atttatttaa tacctgcaga
     4261 aacaaaagtc ccgataatat atttttgaaa aattgcatac aagactaacg gtacaaacat
     4321 tgtaattaaa ctaatcgcca ttgctacggt aaaatttgtc ccaccttcag aactaatata
     4381 ggtctgcatc gccaacggca aagtataatt atctactgtc cgtaaaatta ataccggcca
     4441 aacatactca ttccaagagg taataaaaaa ccaaataccg gtagaagtaa tcgcatgtct
     4501 agtaagagga aaaacaattt tactcatgac ggttgtttgt ttgatgttat ccaatgccgc
     4561 cacatctaga aggctcactg gaatttcccg cattgtttgc gtcaataaaa aaatgcccat
     4621 cgcatcagca aattgtggta ttatcacacc ccaagtagta tcaattaaat taagttttgc
     4681 aatcaacaaa taattaggaa tcatcgcaac agtaaaagga atgaacatcg taccaatgat
     4741 gataaagtac cagattttct tccctttgat tcggcaataa gtaaaactat atgccgcaaa
     4801 aaaagctacg cctaacttaa caaccgttat aattgtagca ataaaaaaag tatttctcgt
     4861 aattttgata attggaagat tttcgaacaa atagcaaaaa ttttccaagg taaacggctg
     4921 tggtatcaaa ctagtaatcg tctgataggc atctttcaat ggcttaaaag aattggccat
     4981 aataaacaat aatggataga taataatgat tatgatgaac ataaataaga gatgtggcaa
     5041 ccagcgttta attttcatag taaaccccct tttccacaaa tttaaatatt acccctaaga
     5101 aaacagcaaa tattaataaa gttaaaactg aaattgctga agattctccg actttataca
     5161 tttcaaaagc cttttgataa gcataataga tcacgtttgc actaccatta ttcggtccac
     5221 cacgagtgat gatcttaatc ggagtaaaca catactgcag accttgaaca atggtcatca
     5281 ccaataaata tagtcctgtc gaactactca taggcaacac aattgtaaaa aaaatgcgcc
     5341 agatgggtac acgatccaac ttagctgctt cgatcatctc ttgatcaatt cccacaattg
     5401 ctgcaaaaat gacaatgaaa ttataaccaa aaactttcca agcagtaata cctgcaatca
     5461 cagcaattac ccaaccttgt aaattcatcc acataggcag tcttaaacca aaatttccca
     5521 acaagacagc aattggtccc gataccggat ttaatatcca tgtaaaaacc atccccgcca
     5581 ctaccaaaga aatcacagct ggaataaaaa ataatggttt atacaaattc tgcccttttt
     5641 ctataactat tcctaataaa aaggatgtaa tatatgggaa cacacagctc agcaagacaa
     5701 atagaacggt ataggacaaa gtattcttca acactttatg aaagactgga tcagtcagca
     5761 cctttacata gttggcaaag ccaacaaatt ctttagtggg ggccaccata ttccagtcaa
     5821 aaaaactcaa atagatcgta taaatcaatg gataaaaaac aaacacgccc aacaaaataa
     5881 ttgctggcgc cagatacaaa taacctacat agcgcttcat ttccgacagc ctcctcatcc
     5941 ttatgtcaaa agcagacgac cttgtttttc ctttcttaca ggcgctgtcg tctgcttgct
     6001 ttgcttattt gagtaactca ttaatttcct tttgagtagc tttcatcgtt tcattgacgg
     6061 aattacctga taaaatttta tcccgcatat ccaacaattt ttgttcagct tctaatccag
     6121 catctccagg aaatgaactc caactgacca tcttatccat ctgatcaatt gctggtatca
     6181 tcatctgatt ttcagctaag aaagctttta atccattttg ggcgtctgca acatctttac
     6241 gtggtggtaa ataaccagta ccttctgtcc atttagctaa agattcaaca ctataaagat
     6301 attttaagaa atcccaagtt gcttcctttt tcttatccgt ttgtgatgta acagctaaca
     6361 ttgcgccacc agcaggtagt ttaacttttt tcccttccca acttggtgac tcaactgctt
     6421 tagcagtaaa agtactattt ttttgaatgt tgtccctttg agcaatagtg gtatagacca
     6481 tcgcgacgtt accatcaata aagctttgca caccttgttc ccacgtagtg tgtaatgccg
     6541 tctggtcttt taccatcctt tgatataatt cataagcagc gataccatct ttagaagcaa
     6601 aggtagcttt gccatctttt attacacttg ccccattact ttctaaaatt gcttgttgtg
     6661 cccaattatc agcaggttct tgaatataca cgccatactt tccagttttt tcttttatta
     6721 tcttagaaaa agtttgaact tcttcccaag tttttggacc attttcatcc aagcccgctg
     6781 cttttaatat atcttgattt aaatataaaa ccggattact aatggagtaa ggaataccca
     6841 cttgtttacc atctttttca gacagtgcca tgatattgtc taaaaaatga tctttaaaat
     6901 aatcagcatt atccggatcc acttcttcta ctaaagattg aggattagta tatttgaaat
     6961 tttctgcaaa ataatcttta aaagcccatc ccacttgtac tacgtccggt atttctttgg
     7021 aggcaacaga agcttgtaaa ttttgcatca agcctttata catatcagga ttatattttg
     7081 ctacaacctt cactttgtct tgagattgat tgtatttatc tactaattca ttcactgttt
     7141 ttccacctag ggtatccgca ttaacatgcc aatactctat cgttacacga ccctccgatt
     7201 tttttgttgt cttggtattc ccactaccac aagctgccaa agagatgaca cttaaaacag
     7261 acacaacccc tataacaaga tttttcttta acataacatt aacttcctcc taatttcttt
     7321 atggccattt ttctaatcaa gaaatattat gaactaccaa tgtaaatttc tagttaattt
     7381 tctgtaaata attcgaaatt tgtgcatttc ggggtacagg gtcactggac atatccatca
     7441 agctaagaat aaagccccca tatggtcatg tatcccatct ccaaatctct taactcctct
     7501 gttttgatta taaaatagtt tattctgtgc tcctttcttc cataaatagg cccatgcaca
     7561 acgtgaggtt atattataaa caccacagcg cgcaaggtac tcaatcgttt ctagttcatt
     7621 gactgtccat acagaaaatg tcaatcctag ctgctgcccc acttcacggg ccaacttagt
     7681 taaccatcct tcatgaatat tgacgatttg aacgctttga taggctaatt tctgtaaggt
     7741 atcgatcaaa tctgcttcgg taatatcccc aagttttttc ccttcgaact gattttcaat
     7801 attataaaat atatgctttc cccaaatcgc agcccaagat aattgtacat agccactaag
     7861 aacaatttgt ggccagattt gataggtttg tgccagcgaa aaaacggcca tttccaatcc
     7921 ctcttctttt aaatcacagt taatggtcat ggttgccttc tcattggcca ctgcttgaaa
     7981 cacctgctca agggttaccg aatctgggac aacttcttac ctgtcatggg ctagtattag
     8041 cgtaccatct gtttgctgac gcacatctac ttcaaaacaa agctggcgat ccaaacaaaa
     8101 gcgaagaaat gcccagctat tctcctccgt attgtcacta ccagagtgtg cagtgatcat
     8161 ggtcattttg acccctcctt taattccgtg attttctccc acacgtccac tataccgatg
     8221 aagcgttaat ttcttgttaa tttcttgtaa attccctgcg ttttttcagt aaactagttg
     8281 taaatcttgc atgctccttc ctacgaactc atgaatcgtc gattttgccc ttctagctag
     8341 tttcaccaat taagtagttc tacattcccc gcttttaacc tactacacct ttttttgttt
     8401 tcagctattc cttcttcttg ctgaaagtcc ttgaggttct ttcttacagg ataccatcaa
     8461 ggtgttcaat ggcttattcc cattgaatga ccgagactta ctatcagatt tctgctcgtt
     8521 gaaccaagtc tttgatacag gatttcttcc gtcatcttct cacaaatacc accttgcgtt
     8581 tctctagcga ttggtcgatg tgcaccctcc cagtggactg tcactacccc tttttgctat
     8641 gcctagccca caaaaaaagc tgtcctcaca gacagccaga ctgtagacaa aagctgttcc
     8701 aaactcgtaa ctcgagcttg gaacagcttt ttatctactt cgtttcgaat ttttgaacaa
     8761 aaaaataaag gctccttgcc tttccatagc caggtggcta atttctttaa attcattgca
     8821 gcaaacacta gcatcgtatg cgtcgcgact ttttccaagc ctcgatactt ggtccaacgc
     8881 atcccatgct tttcttttgc atccgcaaat attcgttcaa tcgtttgttt ccgttttttg
     8941 taaattgatt tattcagatc tgttaggcga agatgttcca cttcatccat cagatctccc
     9001 caaacatgtc tcgtgatg
//
LOCUS       DAZBSA010000104         8944 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000104 DAZBSA010000000
VERSION     DAZBSA010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.105"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..1152)
                     /locus_tag="ACHGMT_002374"
     CDS             complement(13..1152)
                     /locus_tag="ACHGMT_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFN6836089.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1551..2849)
                     /gene="eno"
                     /locus_tag="ACHGMT_002375"
     CDS             complement(1551..2849)
                     /gene="eno"
                     /locus_tag="ACHGMT_002375"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFN6836090.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(2965..3720)
                     /gene="tpiA"
                     /locus_tag="ACHGMT_002376"
     CDS             complement(2965..3720)
                     /gene="tpiA"
                     /locus_tag="ACHGMT_002376"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFN6836091.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(3824..5014)
                     /gene="pgk"
                     /locus_tag="ACHGMT_002377"
     CDS             complement(3824..5014)
                     /gene="pgk"
                     /locus_tag="ACHGMT_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFN6836092.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(5124..6125)
                     /gene="gap"
                     /locus_tag="ACHGMT_002378"
     CDS             complement(5124..6125)
                     /gene="gap"
                     /locus_tag="ACHGMT_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFN6836093.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(6166..7257)
                     /locus_tag="ACHGMT_002379"
     CDS             complement(6166..7257)
                     /locus_tag="ACHGMT_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFN6836094.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(7406..8740)
                     /gene="rpoN"
                     /locus_tag="ACHGMT_002380"
     CDS             complement(7406..8740)
                     /gene="rpoN"
                     /locus_tag="ACHGMT_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFN6836095.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            8800..8871
                     /locus_tag="ACHGMT_002381"
     tRNA            8800..8871
                     /locus_tag="ACHGMT_002381"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8833..8835,aa:Arg,seq:ccg)
BASE COUNT         2320 a         1897 c         1648 g         3079 t
ORIGIN      
        1 attaaaaaaa tcttacgcta agtaggataa aataattcct tgatattttt cgatcatatc
       61 caaataatta tcgatttcaa catactcatc tacttgatga ggcaaagtaa cgacaccagg
      121 cccaaagacg acaaagtcga aggaatggtt agctttcgta aattcggctg catccgttgt
      181 tgctgccgct ccaactaaag gtaatggctg gctgaattgt tgctgaatgc aatgaatcaa
      241 aggagaatct ggatcagctt tgacaggtat tttgttgtaa tcgatcatca attccaaatg
      301 atagtctgtc tcttgattca attcattgac gattgattgc agcaatgcaa tgattttatc
      361 atttgggtat tctggaatcg atcgaatgtt cccttggagc tgcgcatggc ttgggatgct
      421 gttgacttga ttcccgccac tgatcaatgt aacattatgg attgtttttc ctaggacagg
      481 attctcgatt gtttcagcga ggtgattcat ttcagcattt gctttcgtaa tgaattcatt
      541 taggtgattg attgcattat aaccttggtc tggcatagag ctatgcgctt cttttccgtg
      601 cgaggtgact gtatagttga ttgatcccat gtgcgtatac atcaaactgt aattggttgg
      661 ctctccgatg atcaaggcat ctaagtcatc tacatagcct gctttcgtga gctgttcccc
      721 acctagttca cccacttctt ctccgacagt ggctaataat ttgactgttc cgttaaatgg
      781 ttttcctgac tctttcagtt cgatcatggc aatcaccatt gctgccaagc cactcttcat
      841 atctgtagca ccacgtccgt acaaacggtt cccatggatt tctgctgcaa agggtgcata
      901 ggtccaagac gattcatcac cagccgccac gacatccata tgcccagata ggcctaacac
      961 ttttcctgat tggccctttt ggtaacgagc aatcaaatta tctctaccat ctctgtaaga
     1021 aacaatctct cctgttatgt catgctttgc caaaagctta ttcaaatagg ctgctacttc
     1081 accttcgttt ccattcactg atttaattcg gataatctct tgtaaaatcg cgattttttc
     1141 ttcttttttc atctaatcct ctcccttgcc atttcttttt attgtatttg agtgtgctcg
     1201 aattttcaaa taggagcaac caaagtaaaa atgaagaaag agagccactt tatgaaaaat
     1261 atgctgctac ttccctccta ttttatttat acgagaggac ttgcccatag attcctattc
     1321 tactgttcta tccttaaaag ctcttatatg atgctagtta ttgttagctt tcctttttca
     1381 aggatgatct tttcttgtac cattctttgt aaacatcata tacagaagct agttgcaaaa
     1441 aaactctcta cctgctgata tttcagcaag tagagagttc gattacttaa ctaagtgatc
     1501 atactcgact atctttcaat agtcgatcat gtatcttcat tagaaaagaa ttatttgttt
     1561 tttaagttgt agaaagattt caaacctttg tattctgcaa cttcaccaag ttggtcttcg
     1621 atacgtagta attggttgta tttagcaata cggtcagtac gtgatagaga accagttttg
     1681 atttggccag cgtttgttgc aacagcgata tcagagattg ttgaatcttc tgtttcacca
     1741 gaacggtgag atacaactgc tgtgtagcca gcttctttag ccatttcgat agcttcgaaa
     1801 gtttctgtta atgtaccgat ttggttcact ttgataagga ttgagttagc aattcctttt
     1861 tcgatacctt cagataattt ttgagtgttt gttacgaaca agtcgtcacc aactaattga
     1921 actttgtcgc ctaatacgtc agttaatttc ttgaatccgt cccagtcgtt ttcgtctaat
     1981 ccatcttcga ttgagatgat tggatattta gaaactaatt cttcgtagaa tttgatcatt
     2041 tcgtcagttg ttttttcgcc ttcgcctgag tcagctagta cgtaaacacc tttttctttg
     2101 tcgtagaatt ctgaagaagc agcatccata gcaagaacaa cgtctttacc aggtacatag
     2161 ccagcttttt cgattgcttc gatgattact tcaaaacctt cttcgtttga accaaggttt
     2221 ggagcaaatc caccttcgtc accaacagaa gttgctaatc cgcgagcttt caagattgaa
     2281 gctaatgcgt ggaatacttc agcacccata cgtagagctt ctttgaatgt tggagcgcca
     2341 acaggcatga tcatgaattc ttggaagtcg attgagttat cagcatgtga tccgccgttg
     2401 atgatgttca tcattggtgt tggcaatact tttgtgttga atccgcctag gtagtggtat
     2461 aaaggtactt ctaggtagtc agcagctgca cgtgcaacag cgatagaaac acctaaaata
     2521 gcgttcgcac ctaatttacc tttgttagga gttccgtcta aagcgatcat agctttgtcg
     2581 atagccattt ggtcacgaac atcgtagcca atgattgctt cagcaatgat gttgtttacg
     2641 ttgtcgacag ctttagttac acctttaccg ccataacgag ctttgtctcc gtcacgtaat
     2701 tcaactgctt cgtattcacc agttgaagca cctgaaggaa ccattccgcg gccaaaagct
     2761 ccgctttctg tgtatacttc tacttcgatt gttgggttac cacgtgagtc taagatctcg
     2821 cgagcgtaaa catcagtaat aattgacatg ttttgtctct cctttgagtt tgttttttcc
     2881 taagggattt ccctcatata cgattttagt taattttgct cattagtgca aacatttctt
     2941 gcatttttcg caaattttca acaattattt tacagcgtct aacaatgaca agaatgaatc
     3001 tgcttgcaag cttgcgccac cgactaaagc accgtcaaca ttttcttttg ccatgtattc
     3061 agcaatattt tctggtttta cagaaccgcc gtattggata cgaactgctt ctgatacttc
     3121 ttttccataa agtttttcaa ctgtttgacg tacaactcca caaatttcgt cagcgatttg
     3181 tgcgtcagct gatttaccag taccgattgc ccagattggt tcataagcaa taaccatgtt
     3241 tgacacctgt tcagctgaaa gatcagctaa accagctgta atttgtcctt cgatccattc
     3301 agcagttttg cctgcttcat aggtttctaa agattcgccg cagcatagga ttggtgtcat
     3361 accatttgca aagatagctt tcgctttttt attgatgtct tcgtctgttt catggaaata
     3421 ttcacgacgt tctgaatgtc cgatgatcac ataatgaaca cctagatcag caagagctgc
     3481 tggtgaagtt tcaccagtga acgcacctga gttttcccag tagcagtttt gtgctgagat
     3541 ttttagttct gtaccttcag ctgatgtcat taattcttgt aagaataatg caggagatcc
     3601 gatgactgaa tcaactttgt catttgaagg gattttgttt tttactgctt cagcaaattc
     3661 ttttgcttct gatgcagttt tgttcatttt ccagttacct gcgataatag gtttacgcat
     3721 tgaggaacac actccttaat ttattgaaaa atggctaggg agtcatgaaa actcccttca
     3781 ccttttgttc gattactcgt cagaattgag caaacttggt cgtttattta tcgttgattg
     3841 ctgctagtcc tggtaattct ttaccttcaa gcaattccaa gcttgcgccg ccacctgttg
     3901 agatgtgtgt gaatttgtca gcaaatccta attgttctgc tgctgcggct gagtctccgc
     3961 caccgatgat tgttgttgcg ccttctaagt tagcgattgc ttcacaaaca ccgattgtac
     4021 ctttagcaaa gttactcatt tcaaatacac ccataggtcc gttccaaaca actgtttttg
     4081 caccttgtaa tgtatcagca aataatttta ctgatgctgg gccgatgtct aaagccatca
     4141 aaccatcagg aatgtctcct tcgattactt gtgtttcaac atcatttgaa aattctggtg
     4201 cacagacatt gtcgattggc aagacaagtt tgtcgcctgc tttttcgatt aattcttttg
     4261 ctagttccac tttatcagct tctactagag agttaccgat cttgattcct tttgcttcgt
     4321 agaatgtgta agtcattccg ccaccgatca agattttatc tgctttagga atcaagtttt
     4381 cgattacgcc gattttatca gaaacttttg cgccaccaag gattgcaatc attggtcgtt
     4441 ttggttcttc tactgcttca ccgatgaact tgatttcttt ttccatcaag aatccagcaa
     4501 ctgttgggat accagttgaa gcgataccta cgttagaagc atgtgcacgg tgagctgtac
     4561 cgaaagcatc gttaacaaat acatcgccta atgaagccca gtatttacct agttctgtat
     4621 catttttgct ttctttttta ccgtcaatat cttcaaatcg tgtattttcg aatacgacaa
     4681 cgtcgccgtc attcatgttg cggattgctt cttctaattg ttctccgcga gtttcaggta
     4741 cgaatgttac ttctttacct aataattcgc ctagacgttt tgcaactgga gctaaagatt
     4801 ttccttcttt atcttcttct gtttttacac gtccaaggtg agagaaaagg attgcttttc
     4861 cgccttgttc tagaacgtag ttgattgttg gcaatgctgc tttgatacga gtgtcgtcag
     4921 tgatcacgcc gtctttcaaa ggaacgttga agtcaacacg gacaagaact tttttgtctt
     4981 ttaaatcgat atctttcact gttttcttag ccattttatt tcctcctgaa taaacttatt
     5041 atcaaaaaaa gcggaagaag cgcgattgct tccccgcttt taaatgtaca tgtctataat
     5101 ttacacttgt acgtttgtct atcttataag ttagcgaagt attctaaagt acgtactaat
     5161 tgtgcagtgt atgacatttc gttgtcgtac caagcaacag ttttaactaa ttgtttgtcg
     5221 ccaactgtca ttactttagt ttgtgtagca tcaaataatg aaccgtaagt cataccaacg
     5281 atatctgaag aaacgatttc gtctgtgttg tatccgtaag attcgtttga agcttctttc
     5341 attgctgcat tgatttcgtc aacagttact tctttttcaa gaactgttac taattctgtt
     5401 aatgaacctg ttggtacagg aacacgttga gcagctccgt ctaatttacc gttcaattcg
     5461 ggaattacta aaccgatagc tttagcagca cctgttgagt taggaacgat gtttgcagca
     5521 gcagcgcgtg cacggcggaa gtcaccttta ggatgaggtc cgtctagagt catttggtca
     5581 cctgtgtaag cgtggatagt tgtcattaat ccttcaacaa caccaaattt gtcgttcaat
     5641 gttttagcca taggagctaa acagttagta gtacatgaag cacctgagat aactgtttct
     5701 ttaccagtta atgtttcgtg gtttgtgtta taaacgattg ttggaacatc gtttccgcca
     5761 ggagcagaga taacaacgcg tttagcacca gctgttaaat gtttttcagc tgctgattta
     5821 gaagtgaaga aaccagtaca ttctagaacg atatctacgc ctagttcgcc ccatggtaat
     5881 tcttctgggt tgcggttagc taatacttta acttcttttc cgtttacgtt gaatgaacct
     5941 tcatgaactt caactgttcc gttgaaacgt ccttgagttg tgtcgtattt taacaagtgt
     6001 gccaacattt tagcatctgt caagtcgttg attgctacta cttcgattcc atctacatct
     6061 tggatacgac ggaatgctag acgtccaata cgtccaaaac cattaatacc tactttaact
     6121 gtcatttaag atttcctcct tatgaaaatc aaaaaaatat ttattttaaa gggttacccc
     6181 ttttaaaatc tcatttgcag cagcttcatc agtgatgagc cacgtttgtt ttggtgcatt
     6241 tttcatataa gctcgaattg ctttagcttt tgattttccg cccgcgattg ccatgatgat
     6301 cgggactttt tccaaatctt gcaattctat gccgattcta gggaccttat aaacgacttc
     6361 acctttttca ttgaaaaagt agccaaaaga ttccgcaaca gcatttgctt gtttcagcat
     6421 gactaattct ttttcagtca tcttacgacg tgctgccatg tgtaatgcac gcccaatact
     6481 atgaataaca caattcgctt cactaatcaa gtttaagact tcttggatag atggctcatt
     6541 gagtaaagaa ttgtaagttg ccagacttaa ttgttccgga acataaagtg cgcggtggtt
     6601 accgtttgct ttagcagcca ttcttgcact gacagaattt gcctgtacag ttatcgcttc
     6661 accaatcccg cctcttgcag ggacaaacag gttatgccgt ttcttatttt ccagattaga
     6721 caattgttcg gctaccacag ccatagtagt tcctcccatc acagcgatga tattctcgcc
     6781 ttctggaagt agtcggtcta aggcttcgct cagcgcttcg ccgaatgcat cagccacttt
     6841 ggtctgctct tcgctgtttc ctgaaacaac gatacagcgt tggttaccaa aatattctgc
     6901 caactgttgt tcggtttgat gcgtacctaa aagctgatcc atgaagtttt caaggctttg
     6961 ataaacttct tcgcccttag ttgtcagcgt cataccgctt tttgaggtgt cgatcaaatt
     7021 cagatttttc aaaaggtctg tttctgttcg taagactcgt tctgtgatcc ccatactttc
     7081 cgataaactt cgacgcccaa tcggctgcat ccaatagata tttcgtaaaa ttttatagcg
     7141 ttcttgcaac acttcgataa tatctggtgc aacagattca atcattttta tttcatccag
     7201 catagcgatc tccttagttg gacactttac gtccaatgta gacttaaaac gacccatata
     7261 cgataaaaaa agaaattaac tattcgaatt tcacatcaat aaagggaaat ctcaacctca
     7321 cgactgtaag tctaacagtt ttgctctcgt ttttcaacta aaaagactag aaaaagtgaa
     7381 atactttttc tagtcttttg actgattagt tatcataacg ttttcttttt gatgaactcg
     7441 ggattcctaa taaatcacga tattttgcta ctgttcgtct tgagatcgct atctcatctt
     7501 ttttcagcaa ttcaaccaat ttctggtcag acaacggctt gtttttgtct tcttgatcga
     7561 ctaattcctg cagctttttc ttcgctgtat ctgctgaaag gtcttctgtc tgttctgtac
     7621 tattcgtagg aattcgtgta gtgaaaaatt tttttaattc aaatacaccg aagtctgttt
     7681 ccaaatattt cccgttgact gcacgactga ccgtggattc atgcacatct atctcagttg
     7741 caacttcctt taacgtaaga ggcttgattg gacgttcctt gtcgataaag aaagcttttt
     7801 gccgggaaac aatgacttga gcaacccgca aaattgtatc cccacgttgt aggatcgttt
     7861 tcttcaacca ttcgaattcc tgtttccgtt cttttaaata attttcagtt tctttatctg
     7921 cttgttgtga catttgtttg aagtaactct cttgaaagcg tatatttggc tgattttttc
     7981 ggtttgagat aacttgtaca tttttatcgt cgtccactaa tacccggaca tcaggaataa
     8041 tgtacaattc gctgctacgg tcgaaaatcc ggccagggct tggactcaac gtttgtaaat
     8101 agtcgaatat ctgctgaaca gcatgtagat caacagaaaa acgttgtgcg atttctttcc
     8161 attttcgctc caccagttcc tcaaaacatt cttccaacac gatataagct aattccggcg
     8221 cataatcatc tcgttctgtt tgaagcatga gacattcttg caaactacgt gcacctactc
     8281 cagctggttc taattgctga atcagagtca acgcatcgag catttggata ggcgtagctc
     8341 caatctcttt tccggcttct tctaaactaa tcgtcaaaaa tccgttcaaa tcgatgtatt
     8401 ctacaagata aagaacgatt ttcctcaaaa aagtatctcg gtaattcaaa tggacttgtt
     8461 cgatcaatga atcaaataaa gacccttttg tatcaggaat ctgatttagg taatttgtct
     8521 cttctccttc ataagaagat gaagaagctt ttatgtaatc tggctgccat tccggctcaa
     8581 caacttccac caatggattt tccattgctt gattctcaac gaatgccaac agctcctcac
     8641 tgttatattg taaaacttgg atggactgtt gcaactgttg cgtcatggct agtttctgtg
     8701 tctgctgctg tttttgtgaa aaattttgct caaatttcat ttttttgtcc aatccccttg
     8761 taatttttta tctgtttagt tacaattatc ccagtgcttg cgtccttagt gtagtggata
     8821 tcacgtaaga ttccggttct tgagacgggg gttcgattcc ctcaggacgc gttctactgc
     8881 tattttagca taatttacga atagttgctt ctttattttt aagtgaaaac tgtccgccaa
     8941 agaa
//
LOCUS       DAZBSA010000105         8830 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000105 DAZBSA010000000
VERSION     DAZBSA010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.106"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(82..711)
                     /locus_tag="ACHGMT_002382"
     CDS             complement(82..711)
                     /locus_tag="ACHGMT_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFN6836096.1"
                     /translation="MSNRGIYEKFFKRLIDILFSILGLIMLWPLMTFVAILVRIKLGS
                     PVIFKQQRPGKHEKIFLMYKFRTMTDQRDEEGNLLSDDIRLTQFGKILRSTSLDELPE
                     LWNILKGDMSIIGPRPLLVEYLSLYSNEQRKRHFVRPGLTGLAQVSGRNAISWKEKFE
                     KDIEYVENISMMKDIKIFFLTLKKVIVRDGITSVASGTTEKFEGAKIDE"
     gene            complement(704..1822)
                     /locus_tag="ACHGMT_002383"
     CDS             complement(704..1822)
                     /locus_tag="ACHGMT_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFN6836097.1"
                     /translation="MENKRILLASPHMSDEGYEQEYVKEAFDTNWVAPLGMNVNGFEE
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPIIYQGAIPV
                     FVDSDYETWNMNPEHLRTALEKYPNAKAVLVVHLYGLSAELDEITKICKEHNVVLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKKELSKLSNIQF
                     MPENNWNEPNYWLSVITVKGINTNHLIDELDKNNIEARPIWKPMHLQPVFDKYEYIGT
                     DVAQTLFEKGICLPSDSKMTDDDLYRVTNTIKRVMQNV"
     gene            complement(1824..2456)
                     /locus_tag="ACHGMT_002384"
     CDS             complement(1824..2456)
                     /locus_tag="ACHGMT_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFN6836098.1"
                     /translation="MKAIIVGAGGFGKEIAFLLESFSRYELIGFVDDSLKMQNQELVG
                     KTVLGTIDSLLELEEETAIFLGIASPDIKEKIYQKINKNNKLIFPNLVASSALVGINV
                     QLGIGNILMPYTTYTADVVLGNFNMINIGSTIGHDTQIGNYNSIFPSVNISGHVLLGD
                     KNEIGVGTKIIQNLRIGNMNIIGAGSVVIKNINNKTKNVGVPTKVIERWD"
     gene            complement(2467..4284)
                     /locus_tag="ACHGMT_002385"
     CDS             complement(2467..4284)
                     /locus_tag="ACHGMT_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFN6836099.1"
                     /translation="MFVLTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFGSLSASAASSIIILLFINKQYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYLSTDSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVIGFVDDDPNKRNTYLSGKKVLGSLKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESAHVKVNTMPSMEELASGKISVSKLKTIDVVELLGRDEVELDIESI
                     KDQITNKVILVTGAGGSIGSEICRQIIQFDPAKLLLLGHGENSIYLIDRELRTHHQKC
                     PTEIVPIIADIQDRKKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPVTVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSMQTVMEFVKSLPQDDEQLAKDIVTFANASNK"
     gene            complement(4297..5061)
                     /locus_tag="ACHGMT_002386"
     CDS             complement(4297..5061)
                     /locus_tag="ACHGMT_002386"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFN6836100.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLQLGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVAQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(5089..5787)
                     /locus_tag="ACHGMT_002387"
     CDS             complement(5089..5787)
                     /locus_tag="ACHGMT_002387"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFN6836101.1"
                     /translation="MARTQKQKATQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSRDAGYYYGN"
     gene            complement(5799..6578)
                     /locus_tag="ACHGMT_002388"
     CDS             complement(5799..6578)
                     /locus_tag="ACHGMT_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFN6836102.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGSAPARPKATNDKTAPSRRSRSRV"
     gene            complement(6594..7538)
                     /locus_tag="ACHGMT_002389"
     CDS             complement(6594..7538)
                     /locus_tag="ACHGMT_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFN6836103.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTLQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(7584..8363)
                     /locus_tag="ACHGMT_002390"
     CDS             complement(7584..8363)
                     /locus_tag="ACHGMT_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836104.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
     gene            8706..>8830
                     /locus_tag="ACHGMT_002391"
     CDS             8706..>8830
                     /locus_tag="ACHGMT_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFN6836105.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFL"
BASE COUNT         2411 a         1820 c         1497 g         3102 t
ORIGIN      
        1 ttttccgtca gaatttcctc taatatattc gttcgactga tataatcaaa aggatgaata
       61 agcagccact tttttttatt ctcactcatc tatcttcgca ccctcaaatt tctcagtagt
      121 cccacttgct actgatgtta ttccatcacg gacaataact ttctttagcg ttaaaaaaaa
      181 tatttttatg tctttcatca ttgaaatgtt ctccacatat tcaatatctt tttcaaattt
      241 ttctttccaa ctaattgcat tacgaccact gacttgagct aatccggtta aacctgggcg
      301 cacaaaatgt ctttttcgtt gttcattact gtatagtgaa agatactcaa ctaacagagg
      361 acgcggtcca ataatactca tatctccttt taaaatattc cataattctg gtagttcatc
      421 aagactagtc gaacgcaata ttttaccaaa ttgagtcaat ctgatatcgt ctgataataa
      481 attaccttct tcatctcttt gatctgtcat tgttcgaaat ttatacatca gaaaaatctt
      541 ttcatgtttt ccaggtcgtt gttgtttaaa gatcactggc gatccaagct ttattcgaac
      601 taggattgca acaaacgtca tcagtggcca taacattatt aaacctaaaa ttgaaaaaag
      661 aatatctatc aaacgtttaa agaacttttc gtagatacct ctattagaca ttttgcatca
      721 ctcgcttaat tgtattagtt actcgataca aatcatcatc ggtcatttta gaatctgaag
      781 gtaaacagat acctttttca aacaatgtct gagcaacatc tgtaccaata tactcatact
      841 tatcgaaaac tggctgtaaa tgcattggtt tccatatcgg gcgggcttct atattatttt
      901 tatctaactc atcaataaga tgattcgtat ttattccctt tactgttatg acacttaacc
      961 aataatttgg ttcattccaa ttattttctg gcatgaattg tatatttgac aatttagaca
     1021 attctttttt ataaaaacta tgaatatatc gttttttctc aattctttca tttaaaactt
     1081 tcaattgtcc tcgaccgata cctgcaacga tattgctcat tcgataatta tagccaattt
     1141 cattgtgttg ataatggcgt gccttctcac gagattgcgt agcccaaaac ctcacttttt
     1201 ccaccttttc tttgtcatta gacacaagca ttccgccacc tgaagtcgtg attatcttat
     1261 ttccattaaa agaataaatt ccatagtcgc cgaatgtacc agtatatttt cctttatatg
     1321 ttgttcctaa tgattctgca gcatcttcaa ttaaaacaac attatgttct ttgcatattt
     1381 tagtaatttc atctagttca gctgataaac cgtacaaatg cactactaat acagcttttg
     1441 cgttaggata tttttctagc gctgttcgca aatgttcagg gttcatattc caagtttcgt
     1501 aatcactatc aacaaatact ggaatagctc cttgatagat gattggattg gcagtagctg
     1561 agaacgtcaa agactgacaa atcactatat catctttacc gaccccagcc gcttttaaag
     1621 ctagatgtat agctgctgtt ccagaagata gtgcagctgc atgactagca ccgacatatt
     1681 ctgctaattc ctcttcaaaa ccattgacat tcatacccaa aggagccacc caatttgtat
     1741 caaatgcttc ttttacatac tcttgttcat atccttcatc actcatatga ggagatgcta
     1801 ataatattcg tttattttcc aacttaatcc catctttcta tgactttggt aggtacgcca
     1861 acatttttag tcttattatt aatatttttt attactacac ttcccgcacc tataatgttc
     1921 atatttccaa ttcttagatt ttgtattatt ttagtaccta ctccaatttc atttttatct
     1981 cctaatagaa catgtcctga aatatttaca cttggaaaaa ttgaattata attaccgatt
     2041 tgagtatcat gtccgatagt agagccgata ttgatcatat tgaaatttcc taaaactacg
     2101 tcagcagtgt aagtcgtata gggcatcaaa atattgccaa ttcctaactg aacatttatg
     2161 cccactagtg cacttgatgc aactaaatta gggaaaatca atttattatt tttatttatc
     2221 ttttgatata ttttctcttt gatatctggc gaagcgattc ccagaaaaat agcagtttct
     2281 tcttctaatt ctaataacga gtctattgtt cccaatacag tttttcccac taattcttga
     2341 ttttgcattt ttaacgaatc atcgacaaaa ccaatcaatt catatctaga gaaactttct
     2401 agtagaaaag caatctcttt gccaaatcca ccagctccta caataattgc tttcacttta
     2461 ctccccctac ttattcgaag cattcgcaaa agtgacaatg tctttagcca attgttcatc
     2521 gtcttgcggc aggctcttga caaattccat cactgtttgc atggaataac ctttgatgtt
     2581 tcccacaaag atcttctcgt aaactgcttc atcgtttctt tccttatcta acaatagttc
     2641 ttcatatagt ttttcccctg gacggatacc tgtttcaatg atttcgatct cgtcttcgct
     2701 atatccgctc aatcggatca tgttttttgc tagatctacg attttcactg gttcactcat
     2761 atctaaaata aagatttcgc cgccttttgc taacgcccca gattgaataa ctaaacgact
     2821 tgcttcagga atcgtcatga aatatcgggt catacgaaag tctgttacag tgactggccc
     2881 tccttgagca atttgttccc ggaatactgg aatcacacta ccgcgagaac caaggacatt
     2941 cccaaatctg acagctgaga atttcgtaca tccttcttca ttcaacccag tcacgatcat
     3001 ttcagcgatt cgttttgttg aacccatgac gtttggcgga ttgttggctt tatctgtcga
     3061 gaccatgacg aagtttttta ctttcgctgc tttggctgct tctgctacat tttttgtacc
     3121 gaaaatattg tttttgactg cttcttttgg gttatattcc atcaatggta catgcttatg
     3181 agcggctgca tggtagacga tatctgggtg gtattgttcc atgatctcat tgattttctt
     3241 gcgatcttgg atatccgcaa tgatcggcac gatttctgta ggacactttt gatgatgcgt
     3301 gcgtaattca cggtcaatca agtaaatcga gttctcccca tgacctaaaa gtaatagttt
     3361 tgcagggtca aattggatga tttgccggca gatttctgaa ccgattgatc ctcctgcacc
     3421 ggtgacaaga atgactttgt ttgtgatctg gtctttgatg gattcgatat ccaactctac
     3481 ctcatctctt cccagtaatt ccactacgtc gatggttttt aattttgata cgctgatttt
     3541 ccctgaggca agttcttcca tagaaggcat cgtattgact ttcacatgag cagattcgac
     3601 taactcgaag attcttcgta atttttttct tgataaagaa gggatagcga tcgttaccat
     3661 ttggatgtca tatttttcga tcaattccgg tatatctttt aatgacccca gtactttttt
     3721 tcctgaaaga taggtatttc ttttattcgg atcatcatcc acgaatccaa tgacatggat
     3781 atcttgagcc gttttagaac caagaaagct gttgtataaa atacgtccac cttcgccggc
     3841 cccaacgatc aaggtatttt tagcggaatc tgttgataga tacctcatat tttttgtttc
     3901 gatatagata cgccagatca atcggctgcc aatgatcagt aatagcgaaa gtaggtatgc
     3961 aaagatcact aaacgtaaac tgtattgttt atttatgaaa agcaatatga tgatacttga
     4021 ggccgcactt gcagataacg aaccaaaaat cgcaatgatc tctcttaaat ttgtataacg
     4081 attgattcga gtaaaaactt tgaataaact accatagaat aaataaaaac caatcgacaa
     4141 accgatagag attaaaataa ggtccattgg tatagctaca aacggcttca tgaatttagc
     4201 tgctgcaata ttcgcagcaa tcaacaacaa gctgtccaca aataccaatg tggcaatttt
     4261 tcttcttctt gttaatacaa acaccctaca ttccccctaa aataaaccaa acttcttctt
     4321 ttttacctct tcatagactg gataactgat ctcatccccg ttgatcaggt cctttgcgac
     4381 ttgtttcatc tgtgctactt tttccttacc aaagtccttg gcgatttgct cataacattc
     4441 ttttaagtaa aaggtacgtt tagagacccc atgggcatcg gaagcagcca tttgcacgag
     4501 gttatgtttg accatcattt tggctgtttt ctggatgtct tttccaaata caccgacata
     4561 acttggtgcg gtgagttgag ccaagcatcc catatctagg aatggcagca aatgatttgg
     4621 gtcttctcga aaatgcgcgt tgcgttctgg atggacgatc actggtgtct ttcctagttg
     4681 taacaactca aaaaagagcc attctgcata taacggcact tccagtgtcg gaaattcgat
     4741 tagaagatat gtatcatcta aatctgtaaa taaaatttca tctcggtgga tgtcttcaat
     4801 cagtgttccg gtaatacgta cttcttgtcc ttccaataac gtcaaaggca attgtctttt
     4861 ctctaattct gcttgtaacg aagcgactag agaaatgact tgtgattttt cattgctgta
     4921 ttttccatta ttgtgatggg gtgtgcataa aatatgtgtg atcccttgtt gaatcgcttt
     4981 ttcagccatg gcgatactgg catctatgtt ttctgctccg tcatcgattc ctggcaggat
     5041 atggcaatgt agatcgatca tttctcttgt ctcctctctt tttatttctt aattcccgta
     5101 gtaatagcca gcatctctgc tgtgttctgc tccattgtat acaacaccaa ggactcgcgc
     5161 ttgtaccata tctaacagtt cttttgcttt agtcaatgat tcttttcgtg acgtattttc
     5221 ccgtacgacc aataaggtgc catccgtttt tgaagacatg atctgtgcat ctgttacggc
     5281 aacgactggt ggcatgtcga aaatcactac atcaaatagt tgtcttgctt ctgctaaaat
     5341 ctgatccatt cttggcgaac ttagtagttc tgatggattt ggtggtttag gtccacttgg
     5401 cagaatcgac aaattgtcga ctggtgttcg ttggatctca tctgctacac ttcctgatga
     5461 actaagaaca gtacttaatc cgcttgcgtt gttcaacttg aacgttttat agatgacggg
     5521 cttacgtaag tctgcatcga ctagcagtac ccgttgacca gatttggcaa aaacaacggc
     5581 aatgtttgct gcagtggtgg attttccttc tccaggacct gatgaagtca cgacaatcgt
     5641 tttgatttgc tgaccagctg cggaggcaaa ctggatattc gtccggatcg tacgatattg
     5701 ttcggcgatt ggtgaagatg ggtttgttaa tgtgatcaaa ctgactgcat gagtttgagt
     5761 tgctttttgt ttctgtgttc gtgccatttt tcttcctcct agactcttga gcggcttctg
     5821 cgtgatgggg ctgttttatc gtttgtagct tttggtcgtg caggagctga tcctttttta
     5881 cggataccag cgttcaattc ttttgctgac atttctggaa ctgttcctaa gattgggaat
     5941 cctaatgtat ccgtcacata tttttcatct ttcactgtcc gatcaagtaa ttcgaataag
     6001 aatgccagac ctacgcctac catcattcct aacactaagc caatcactag attcaattta
     6061 ttatttggcg aaacaggtgt cgtattggca actgcacctg aaataatcga gattttatcg
     6121 acattcatca cgtctttggc gttttcttgg aaaacttgtg aagttgtatt agcgatctgt
     6181 tgagcagtca ctgcattcgt gcttattgcc tggatcgaga acatttgtga attttgtgac
     6241 tgattgacgg agatcgcatc tttgatttgt ccagcagtca ttttcacatt gtcttctgtt
     6301 tctaaacgat ctttgacttc atttagtact aaatcactga cgatcatatc tttatacgta
     6361 ttgatcattt gcaggttcgt attgacatca ttgacatttg ctgtgtctga ttgtggcagt
     6421 gtcacgatca attgtgcttg tgaactgtat tttggtgtca agatgaaaaa tgttacgatt
     6481 cctgagatgg ccaagccagc aaacattgag atgatgattg ttaccaagtg tttgcgtaat
     6541 gtttcaaaaa tttctttcaa gctgattgtc tcttccattt tttctccact ttcctattta
     6601 ttctctagat tcatttgatc ttttaattct tttttcacac gatcaagttc ttgatcgctg
     6661 actttttgat aggagatatt atcttgcatg aagccttctc cttgcagctg atcttgcttg
     6721 atcgtaccaa atgcctcccg ataatctaaa gcgatttttt tcatttcatc aaagctcatg
     6781 tctgttttca tattcgactc tagagcattc aaaacggatt gatagttgct gacaccgttt
     6841 aaactcaata ctttttttgc tacaccttct acgatctttc tttgacgctc ttgtctacca
     6901 tagtcaccat ttggatcttc ataacgcata cgagaataag ataatgcctg atccccgttc
     6961 aatgtaattt taccgattgg aaagtcatag ccatcttgtg agaaagtgag gtcattattg
     7021 acttcgatac caccaactgc atcaacgagt tcctttagcc ctttcatatt gatagagaca
     7081 tagtgatcga ttgggatgtc tacatatttt tgtagggtat ccatcgccat ggcagctccg
     7141 ccgaatgcgt aagcgtggtt gatcttgtct tttgtgtcac gacctacgat gtcgacatac
     7201 gtatctcgag gaatgctgac gatcgttgtc tgtttgtctt caggactgac tgtggcaacc
     7261 atcatcgtat ctgatcttcc ttgatcgacc cgacctaaat ctccagtatc aatgcccatc
     7321 aatagaacag agaaagattc ttgtttgtcc agatcaactg gtttcgttcg ttctccttgg
     7381 gttcgttcca ctgattcgta agttttctca atagaatgtg agagatccag atataatttt
     7441 gctcctactc ctacaattgc taatactaat acgataagaa ctgctaaaat aatcagaaat
     7501 atttttttcc ctcttgattt tcttcttcgt cttgccatag tttcctccgt ttgctaacct
     7561 tgcttgaatc aacgttttaa ctgtcaataa cttatattgc tatagcccat ttgctgagca
     7621 acagtttgtg cttttgttgc gtatgctgcg cttatttttt cattccggat ctgctctatt
     7681 tgactgagcg ccgtcaaata ttgatcgtaa gtagcggtgg cagtcacttg accatccttc
     7741 aacatcgtat cgaataactg atctagttgc gtgacttgcg cgatctgagt atcagcgaat
     7801 cctagatatt gcataacaac cgttttccaa ggactatctt taaaaaagct catattttca
     7861 cgtacacgag cgacatctgc acttgtcacc ttttctttga tgacgacatc gtctacagct
     7921 tgctgccagt ttgatacatt tttagtaaat aaatcgttga cagctgtctg gatcttcagt
     7981 tttgattctg cactatctaa ctgtgtcacg attgctttct tctcatcttg gattgcaaca
     8041 gcttctttag gcaattctga ttctttgatg tcgtaatcat ctgctgaaag tttgacagag
     8101 gatagcttat ttgacaaact tgtgattgtt tcttctgtta tttcagggtt tagaaaattt
     8161 ttttcttttg tactatacag cttgtctact tcttttgata aagaagtcaa ggtgtctttt
     8221 tgattggtga tggcagattc tgcataattg atccgttgct gatgttgccg ctgattattg
     8281 atatggtaac caaacaaaca gatcgcaata gcacctgcac ctaccgccac agcagtcaat
     8341 gacttgacga atggcttttc catagattca ctcctaagta ttgatgatag ataaattaag
     8401 atataattca catacataaa aatgacagct ttttatttta aaaaacataa ttaaattaat
     8461 atattcacag aaatatttgt aaacaagttt tccctatata atcataccac aaattgtttt
     8521 cttgactagg gcaatttacg atttaaaagt aaataaaaca ttaataagat aacaattaat
     8581 cgatttcatt tttatttcct taattttcat agagagtgta aaatattttg tgtaaataga
     8641 aaaaaggaag tcccttctgt agaatagagt taccacaaca cattcacaga aaagaggact
     8701 tccatatgaa cgattttact acagaaattc taaagactct agcgaacaaa ggcgatttga
     8761 atgaattatt ccgtgtccat ttggaaaaag ctgtcaatac gcttctcaaa acggagttaa
     8821 cggctttcct
//
LOCUS       DAZBSA010000106         8659 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000106 DAZBSA010000000
VERSION     DAZBSA010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.107"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..1136)
                     /locus_tag="ACHGMT_002392"
     CDS             complement(93..1136)
                     /locus_tag="ACHGMT_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFN6836106.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(1513..2478)
                     /locus_tag="ACHGMT_002393"
     CDS             complement(1513..2478)
                     /locus_tag="ACHGMT_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFN6836107.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            2712..3557
                     /locus_tag="ACHGMT_002394"
     CDS             2712..3557
                     /locus_tag="ACHGMT_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN6836108.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            3748..4935
                     /locus_tag="ACHGMT_002395"
     CDS             3748..4935
                     /locus_tag="ACHGMT_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFN6836109.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            4941..5297
                     /locus_tag="ACHGMT_002396"
     CDS             4941..5297
                     /locus_tag="ACHGMT_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFN6836110.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            5299..5637
                     /locus_tag="ACHGMT_002397"
     CDS             5299..5637
                     /locus_tag="ACHGMT_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFN6836111.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    5713..5969
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6032..7066
                     /locus_tag="ACHGMT_002398"
     CDS             6032..7066
                     /locus_tag="ACHGMT_002398"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFN6836112.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            7059..7745
                     /locus_tag="ACHGMT_002399"
     CDS             7059..7745
                     /locus_tag="ACHGMT_002399"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFN6836113.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            7769..8617
                     /locus_tag="ACHGMT_002400"
     CDS             7769..8617
                     /locus_tag="ACHGMT_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFN6836114.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEEKPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
BASE COUNT         2882 a         1572 c         1853 g         2352 t
ORIGIN      
        1 gttgttgtta gaaggatatc tgcaaaatta aaaacagttg tgattcggac attgttccat
       61 gcgagaatca caactgtttt ttcatgttca ttctaaactt tgatcagcca aataaatagt
      121 aaagccatca ctgtttggag aagcccgaca gctaatccat aagataagaa gcgtgtacct
      181 tctttcatga attttgcaaa gtctaaacgc agaccaattg ccgctaaagc agtgatctca
      241 aaccagctgc tgataaaatg tgccgattgg tcaataacta caggtaaaga aaacaagctg
      301 ttgaccaaac aaaagatcac aaaccctaat acataccaag gaagcggtaa ttttcgtttt
      361 ttctgcgtat cagttggcgt ttcaaacaga gctggttgtt tcttgtgcat gaatttttca
      421 aacagataga caacaacaac gagcatcata atacgtagaa tcttaaatag catggcgaat
      481 tcgacaacag aattgtcaag taagcttgcc ccagcgacta cttgaccaac agattggagt
      541 gtaccaccca aaagtgcact tttttcaatg agatttccac caaacaactg taatccaaga
      601 aaagggagag tcaacatcat cacagtaccc agtagattga ctagtgtgat gatctgtcct
      661 ttttcttcct ctttcgcatg gatggaaggg gcgattgaag caatagcaga agaaccacaa
      721 acagcgtttc ctccagccat catcaaagaa gaagtatcgc tgaattgcag ttttttacca
      781 atcaagtaag caccgatgat agtgactgac atttgcagta acacaaaaac aattcctttt
      841 acaccaattt gagcaatcgt ttgagctgtg acagttgcac ctagtaaaaa aacagagaat
      901 tctaatagtt tgctttcagc aaattttgtg ccgattgcta gaacaggctg tttaaaaaaa
      961 gtatttccta ataaaatacc caataaaata gcaatagtcg ctccacctaa ctgtggcaga
     1021 taaatagcaa gaagcctgct gaaggcagcg atggcaaaag aaagcagtaa accgggtaag
     1081 atttcgcgga ccgctgaatc ctttacagat tttattggat gtgacaatga tttcatttga
     1141 tcggttcctt tcgtcaataa atactaaaag catactcgaa catagtagca ctaaaccagt
     1201 attttgacaa aagaacaaga ggtcgtgaca aaagtcttgt acgacctctt gtttcgaata
     1261 aacggtgttc cagaagtagc ttctacggaa ataagccgaa atttcacaaa aatttgagaa
     1321 gcaattttcg taaattcctt cttatttctt gaagctgacc acttctttta cgacctcttg
     1381 gtaaacggtg ttcaaaagct tgcatctgcg gtaataagcc caaccagtac aaaaacatga
     1441 acagctgttt ttgtttggcc gttcttatta cttgatgcag aacagctttt tcacaaccta
     1501 ttgaatcgat tattaagcga tttttttaga aacaaatttt ccatgtttgc tatgttcttt
     1561 aattaagaaa cgtacgtaat gagcaagaac gtagaagaca actacacttg caaacagaca
     1621 ttccgcaact tttaagaaat ccaatacgct actatagata cctaaactgt tgaagtaatg
     1681 aacagtagta aatatagctg ttgcagagat aacaagcggg aatgtaaagg ctgcatagct
     1741 tggataaaaa cgaagcttga acaaacgtgg aagcatagac agcacaacga aatacaatac
     1801 ttgagataaa gcgaacaatg ctacgacaag ataaaaatta ggttcagcaa agtttttaag
     1861 ataacctgtt aggcaaagag aaccaggtgc tgcaacgatc gtaatcagcg gacgtgtcgg
     1921 atgagccata tttcttacga aaaagacacg tttgatgatg atcggcaata agaacgcata
     1981 gcagatcaat gaaatccaaa agaaaatttt tccaataagt gggaagaagt tcccggcagt
     2041 tacagtgatg accccaaatc caacgtaaat gataaaccag cttggataaa ttgcttcaat
     2101 tgaaagatca gctttgataa catggtgata attgaaataa aagacaagga tcacttggaa
     2161 gataacagcg cacagccaaa tgaatttgac aattggtgca atactttcat atgaaacaaa
     2221 ataagtacaa ataaccatga gtgacatcgt aaaagttggt gaaacggagg cgatgattgg
     2281 atcatgcaac gtttgtttcg tgtgttcgaa taagataaac aatttaacaa aaatgaatat
     2341 cataagaatc atagagatac cgccagttag attgcctaaa gcaacatggt gatagtcttt
     2401 aaacagattt ccaagggaag ctaacccaag gatcaacccg cagatcggga tcggaataat
     2461 tttcaaaaag ttcttcatat ataatattct cctttttatt taatagattt tagtaccaag
     2521 atgaacgaat acattgtaaa atgaatagga atgtatgatt acgagcaagc agacagctga
     2581 ttgtcttcat ctaagttact agttggatca gcaactgttt tgttgtacat ttctattata
     2641 gatggttttt tgtatattta aatcaaatag tttttatagt aattatcata aaaagttatg
     2701 gaagtggtgc catgtttaag ttattaacga cttttcgagc agtatatgaa acaagaaatt
     2761 tttccagagc agcagaagta ctgtttttat ctcaaccagc agtctcgaat caaatcaaac
     2821 agttggagcg ggaattgaat atcgatcttt ttcaacgtaa cggaagaaaa gagatgcttc
     2881 caactaagca agccgactta ttatatgaac ggacactggt tttgttagaa gaatgggaag
     2941 aaactcagca aaaattgctc aatgaacaaa atgaagaaga agtatgtcgg atcgttgctt
     3001 cccacacctt tgctgtctat cttttgcctc agttgatgaa acatctgatc aaaaggtttc
     3061 ctaaagtgca ttttgagatc gaaatcacga attctcatga atcattagaa caagtagcga
     3121 aacatgaatc cgattttggt tttatcgaaa agccattaga gacttccggc gtacaaagat
     3181 actctttgat ggaagaccag ctagtcatcg ctggggaccc aaaaagcaaa ttgtggcttg
     3241 taagagaagc aaactctggt gtttttcatt acacgcagcg ttattttgaa gagatgaata
     3301 tccaagataa gaagatgatc gtgaaaagta acgatgtaat tgttgcgttg ctgaaggaag
     3361 gaatcggtcg ctcgattttg tctaagcgct ctgttccaga aaatgtgcca atgactccgt
     3421 tgaatttcca tcgatatttt tatttcatcc aacgaaccaa tctggtttct gatcagctgc
     3481 tagagattgc cgaagagatc cttcgataca atgaaagcac agaaacttct cacagcgagg
     3541 agaatctcag ctggtagata attgcatgta tcaaaaaaag ttctatgata cactaaagat
     3601 aagcagtagg cttttgtact gcatttgctc aagcaactgc acaaacggaa agttaatttt
     3661 tcccaaatgt ctatggaaag actttccatt atctaactgg gccgctataa taatcagcag
     3721 tatggttttt aagaaaaggg atgaaacatg gaaaaaaaat taaaagcttc gaacgataat
     3781 cgtattgatt atggcgtgat tttaccagtc tttctgttat gtttgattgg tctgctctca
     3841 ttatatgtag cacttagcca tgatccgaac aatccagcag tcttgaaagg cgtagggatg
     3901 caagttgtct ggtatttagt tggagcggca gcaatcgttg tcatcatgca tttgaattcc
     3961 aaatggatct ggaaactgac tccttacctt tatggtttgg ggttagtggt aatgggactc
     4021 ttgctcaaat tctatgatca gaatttagct gcttcaacag ggtctaaaaa ctggtttcga
     4081 ttcggtgcgt ttactttcca gccagcagag ttgatgaaga ttgcgtacat cttgatgatg
     4141 gcactgatcg taactaaaca taatacgcaa gtaaaagaac ggacgatgaa atcggacttt
     4201 tggctgattg ggaagctctt gatcgtgaca ataccggttt tagcattgat catggcccaa
     4261 gatgactttg gaacgatgct tgtctttctt gcgatctttg gcgggatttt cctgatgtct
     4321 ggaatctctt ggcgcatcat cgcacctgta gtaatcttgg cagtagtcgt tggagcagga
     4381 acgatcttct tggttacgac tgaaggcgga agagacttgc tttataaagt tggattcaaa
     4441 tcttatcagt ttgcacgtat tgattcatgg ctggaccctt tccatgatac gtcaggaatg
     4501 agttatcagc cggcccaagg acttttggca attggtaccg gtggcttgtt tggtaaagga
     4561 ttcaacgtaa gcaatatcta tgtgcctgtt cgtgagtccg atatgatttt taccgttatc
     4621 ggtgaaaact tcggctttat cggtggtgca ttcgtcattt tcttgtattt tattttaatc
     4681 tatcgtatga ttcgggtgtg tttcgatacg aataatgaat tttacgccta tatcgcaagc
     4741 ggtttgatca tgatgctgct gttccatgtc tttgagaata ttggagcaaa tatcggatta
     4801 cttcctttaa cagggatccc attgcctttc attagtcagg gaggatcctc gattttaggg
     4861 aacatgatcg gtatcgggtt gatattatcg atgagatacc aaaatgaagc acctgcaaga
     4921 tcttcaagga ggtaagaaaa ttgtacacct tttactggta cccgaaatgt tcaacatgca
     4981 aaaaagccaa agcgtggtta gaccagcatg gcgttagtta tcaaaccgtt gatatgatca
     5041 aagaagtacc gaaggcctca acgattgaga cttggctaga aacgagtcaa gtaccaatcc
     5101 gtcgcctgtt caacacaagc gggatgaaat atcgggaact tggactgaaa gatcaagtag
     5161 gcgagtttac ggcttcaaaa gcgagccaag tcttatcgac agatggtatg ctgatcaagc
     5221 gaccactgat cttacgagag gaccagcttg ttgcaattgg attcaatgaa aaaatttatg
     5281 aaggagaact gatataaaat ggcaggcaag catttgaaga aaaaggacaa cctttggatc
     5341 ttatttaatg gaaaagaata ctgtgttggt ttgacaaatg aagcgcaaga agaacttgga
     5401 gacatcacgt ttgccaatct gccaaaagca ggacaatcgc taaaaaaaga tgaacctctg
     5461 atcgaagttg aagcggaaaa agcggtcaat gaatttgcca gcccgctaac gggtacagtt
     5521 tcttctgtta atgataaaat cggtgaagat atcaatatct tgaatgattc agatgaaatg
     5581 aatgcgtgga tcttaagttt caaagatgtt gatccaaaag aatttgacga attataaaat
     5641 cagaaaatca cttgaaaaaa gttgacactg attcttccag ttgctataat cataagcaat
     5701 cctatatgaa acgagaaagc tttgaaaaga agagtacatg tcacggacgt ttgaagagag
     5761 ccctgtttgc tgaaaatggg taacgaaaat gagatggaag atggtcttag agcagagtaa
     5821 gcgagcgtaa gcaaacttac tacgggagtg cccgttacag cgcgacagta tacggaaaga
     5881 agctaagtcc aagtactgga agaggctgtt attgcgaagt aacggtaaat caaggtggta
     5941 acacggaagt tcaagctttc gtccttttgc ttaaggcaag agggcgaagg cttttttgtt
     6001 tgaaaaatta taagagaaag atgtgaatgg catgtcatta atccaactaa cgaatgtcaa
     6061 aaagacattc aatgggaaaa acggaaacat ccaagccgtc aatgacgttt cactaaacgt
     6121 tgaaaaaggg gatatttatg gaatcgtcgg ctattctggc gcaggaaaaa gtacattagt
     6181 ccgtctcctg aatggacttg agcttcccac gagcggcgaa gtgatcgtaa acgatcaaga
     6241 tattacaaaa ttaaaaaaca aagaattacg tacattccga aagaaaatcg gtatgatctt
     6301 ccagcatttc aacctgttat ggtcaagaac tgtacttgaa aatatccagc tgccgctgga
     6361 attagctggc gtaccaaaga gcaaacgtaa agaacgggca gaagaactgt tgcgtttagt
     6421 cgggttagaa ggacgcggac aagcatatcc ttcacaactt tccggcggac agaaacaacg
     6481 cgtcggtatc gcacgagcat tggcaaatga tcctgagatc ctgctttgtg atgaggctac
     6541 tagcgcattg gacccgcaaa caacagaaga agtattagac ttattgttag cgatcaataa
     6601 gaaattgaat ttgaccatcg tcttgatcac acatgaaatg aatgtgatcc gcaaaatctg
     6661 caacaaagtc gctgtgatgg agttggggaa agtcgtagaa gaaggagacg tattgactgt
     6721 cttcagacaa ccgaaaaaag aagtaaccaa acgctttgta caacaagaca tcgaaccaga
     6781 agctgattcg acagaactat tagctgaact catcaaagaa aatccaagcg gccgtttggc
     6841 aactttgacc tttagcgaaa gaaatgccaa cgagccagtc atttcccaag cgattcgacg
     6901 ctataacatc gatgtcaacg ttgtgtatgg aaagatcaaa caagtgaaag aaggttcctt
     6961 tggttcattg actgtcttga tgactggtga aaccaaagaa ttagatcaag cagaagtgtt
     7021 catcaaagaa caaggagtag gaattgaggt gatccatcgt ggataaaagc ttttcagaaa
     7081 catatctaga ttttaaacaa atcaacccgg aaacgatgaa tcaggctatc cttgatacac
     7141 tatatatgac attaatctca atggtcttag tgacgatcat cggattagtt ttgggattga
     7201 tcctttactc tattggacgc aaaaacaaac cgtatgctcg aatcatttat ggaatcgtat
     7261 cgatcatcag taatattttc cggtcaacgc catttatgat cctgatggtt ttgatcattc
     7321 catttactaa agcaatcgta ggcactatgc taggtgcgca agcagccatt ccagctcttg
     7381 ttttatccgc agcaccattt tatgcccgtc tagtagagat tgcttttcgt gaagtcagcc
     7441 ctggtgtact ggaagcagca gatgcaatgg gagcaagtta ctgggaaatc atctggaagg
     7501 tcttgatacc tgaaagtatt cccgcattga tttctggatt aacagttaca acgatctcga
     7561 tgatcggatt tactgcgatg gccggcgcga tcggcgcagg tggacttgga ggtcttgctt
     7621 ggcaggaagg ctaccagcgt ggtaatctaa cagtgacatt cgttgcgact ttaattattt
     7681 tactaatcgt atttatcgtt caaggaatcg gtgatttctt aactaagaaa acagacaaaa
     7741 gataatagga tataggggga tgtaggatat gaaaaagaaa atttttggat ttgcagcagc
     7801 gcttttgctg actgtaggat tagcagcatg cggtaacaac agcgacagca aagattcagc
     7861 aaataaagca gacgacacga cattgaaagt cggcgcttca ccgacaccgc acgcagagat
     7921 tttggaacat gtgaaaccat tattgaaaga tgaaggaatc gatcttgaaa tcgtcaaatt
     7981 cgatgactat gtattaccga accaatcttt ggaagaaggc gacattgatg ccaattactt
     8041 ccagcatatt ccttatttga acaaagaaat caaagaaaaa ggatatgact ttgtcaatgc
     8101 cggtgctgtc cacatcgaac caatgggctt gtactctaaa aaagtcaaag atgtttccga
     8161 attaaaagat ggtgcaacaa tcattacttc aaattctgaa tcagactggg gacgcatcat
     8221 cacaattcta caagatgctg gcttagtcaa agtcaaagat ggagtagatc tggaaacagc
     8281 aacattcgat gatattgaag aaaaacctaa gaacttgaag ttcaaccata cgatcgaccc
     8341 atcactttta gcttctacgt accaaaatga tgaaggtgac ctagttgcaa tcaacgcaaa
     8401 cttcgcttac ggcgcagggt tgaatccatt agaagatgcc gtattgcttg aaaaggacaa
     8461 ctctccttac gtgaatatta ttgcgactcg taaaggtgat gaagacagtg acaaaatcaa
     8521 aaaactgatt gaagtgcttc accgcaaaga agtccaagaa tggatcacag acaaatgggg
     8581 cggctctgtt aaaccagtaa aggctgatgc aaaataaggt atggaacaaa aaagatgaga
     8641 aataagtctg taatggctt
//
LOCUS       DAZBSA010000107         8493 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000107 DAZBSA010000000
VERSION     DAZBSA010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.108"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            80..781
                     /locus_tag="ACHGMT_002401"
     CDS             80..781
                     /locus_tag="ACHGMT_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6836115.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            791..3502
                     /locus_tag="ACHGMT_002402"
     CDS             791..3502
                     /locus_tag="ACHGMT_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFN6836116.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            3953..5581
                     /gene="treC"
                     /locus_tag="ACHGMT_002403"
     CDS             3953..5581
                     /gene="treC"
                     /locus_tag="ACHGMT_002403"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFN6836117.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(5641..6822)
                     /locus_tag="ACHGMT_002404"
     CDS             complement(5641..6822)
                     /locus_tag="ACHGMT_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFN6836118.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            7074..>8493
                     /locus_tag="ACHGMT_002405"
     CDS             7074..>8493
                     /locus_tag="ACHGMT_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HFN6836119.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAG"
BASE COUNT         3012 a         1488 c         1716 g         2277 t
ORIGIN      
        1 tggtaaaata aataagtgtg agaaaacgca ttcgatgcat agatatagcg agaaatatcg
       61 tgaaaatgga ggaatcagag tgaagtatgt cgaagtaaaa aatgaataca aacgatacaa
      121 aatgggagaa acgaccatta cagcaaatga cgatatttct ttttcaattg aaaaaggcga
      181 attagtgatt atacttggtc caagtggagc aggaaagtca acgattctta atattttagg
      241 tgggatggat acacctgatg aaggtcagat cattattgat gacacagata ttgcgcaatt
      301 ttcagataaa cagcttactg cttaccgacg aacagatgta gggttcgttt tccaattcta
      361 caatctcgtt cctaatctaa cagcaaaaga aaatgtggaa ttggcaacgg aagtttctcc
      421 tgatgcgctt gatcctgtag aggtattgag gcaagtgggc ctagctcatc gattgaataa
      481 ctttccgtcc caattatcag gaggcgaaca gcagagggtt tcaattgctc gtgcactagc
      541 taaaaatccc aaacttcttt tatgtgatga accgacaggc gcgctggatt ttgaaacggg
      601 caagcaagta ttgaaattat tgcaaaacgc tagcagacaa cacggcaata ccgttttgat
      661 cattacacat aattctgctt tagcgcccat cgctgataga gtcattcaca ttaatgatgc
      721 taaagtaagg tctgttgaat taaatgatca tccttcatcc attgatgaga ttgtatggta
      781 gggggatttt atgaaaacaa gaacatatct taaagcaggt tttcgcgaaa ttcggcaatc
      841 taaaggacga ttcattgcaa tcatccttat tgtcatgcta ggaacacttt tatttgtagg
      901 tgtcaaaact gctggacctg tcatgcaaaa aacgatggat cactatgttg gtacagcagg
      961 actttctgat ttgcaaatcg tttctactgg tggcttgaca caaaaagata ttgctcaagc
     1021 agaaaagatc ccagatgcaa aagtagaaac aggaaaacag atctattatt ccaatactgg
     1081 caaaaacgaa gtgattcaaa tattttctta taacaaaaat aacaagcaga acaagctaca
     1141 attaactgat ggaaaactcc cggaaaaacc aaaccagctt gtattggatg aaaaagcgga
     1201 agatgaaggg tataaaatcg gtgatattta tcagattgat tcagatgaat tgaaagaaaa
     1261 agagtatacc atcacgggct ttgtccgttc tccattgttc atcaacaatt tggagagagg
     1321 atacgcgaac gtcggaaatg gtagcgtcga ttattttgtc tatttacctg aatccgtttt
     1381 taaaacagat atttattctg tcttatatct tgattttaca aacgtaaaga atttgaatac
     1441 gtacagcaaa gcttacaaag ataaaatgga gcaaaatcaa gaaaaggcag aaaagtattt
     1501 acaagatcga cccgaagaac gcttagaaga gctgaaaaaa tcggccaacg aagaattaga
     1561 accggcacag caaaaaatag cggatggcaa agcacaaaca acgcaagcaa gaacacggtt
     1621 agaagatgct aaaaaacagt tagcgcaaca gtcagcaact atccagcagc ttcctgaagc
     1681 gcaacgaaca atcgctgttc aaacattatc tcaacaagaa gctcaacttg ctgaacaaga
     1741 aaggcaatta gttgagaaag aacgtgaact ggcctctgca gaggaagaat tgaatcgaaa
     1801 tgcagaaaaa attgaaaatt taactaaacc atcataccta tacaatgaac gttcagaaaa
     1861 tgtcggtttt caagaatttg gtgatctgtc tgatcgtatc gctgcgattg ccaatgtttt
     1921 tcctgttttc tttttcttta ttgccgcatt gatcaccttt acgacaatga cacgtatggt
     1981 agaagaaaac cgcagggaaa tcgggacatt aaaggcgtta ggttatacga aagttgaaat
     2041 cgcaaaaaaa tatgcaatct atgcaagtct agcctcaggg ataggtattg ttttaggaac
     2101 gatattagga acgaatcttt tgccaaggat catttttgag ctgtctaacg agcggtatga
     2161 tattgggagt gcgcttattt tttatgactg gccgccgatt ttacaagcag ctgccgcatt
     2221 cttcattgcc gcatttggcg ctgcaatgat cgttttattc aaagatttaa gagaacggcc
     2281 tgctgcattg cttcaaccga aagcaccgaa gccagggaaa cgcatttttc ttgaatacat
     2341 cactccgctg tggtcacgcc ttagctttaa tcaaaaaatc agttaccgaa atctcttccg
     2401 atataagtca agaatgctta tggcgattat cggaattgct ggatgtgccg ggctgatggt
     2461 tgcaggcgtg ggattgaaag attctctaag ttctgtttca gccaaacaat ttggaccgat
     2521 cattgactat caagcaatcg ttacattgga ttcatccacg gaagatagtc gtcaaaaagc
     2581 agcggatatg ctgcagaagc aagaaaaagt aaatgagatc ctagctattc atactcaaac
     2641 cgtagaattg agaaaaaaag gacatgcttc gcaaagtctg acagtgatcg taccaagtaa
     2701 aacagctgat ttggatcgtt ttatccattt aactgatctt gaagagaacc cacgctcctt
     2761 gcctaaaaca ggaatcgcta ttacgcaaaa agtagcagaa ctatttgact tatctacagg
     2821 agatcatatt tcgctttatg atgaagatca aaaagaatgg aaagtcgaag taaaagaaat
     2881 cgtgaaaaac tatctaggta gctttattta tatgtcccct gaatattatg cttcagttac
     2941 tgatcaatcg atgatggata atgcattttt gctgcgtaca gataagatga caacaaaaga
     3001 agaagaaatg ctttccgaga aactgcttgc cacagacgct gtgacaaata cgtctttcat
     3061 gtccaaacaa atcgaaacgc aagaagaatc aatggcgaac ttagactcgg ttgtcttaat
     3121 ctttgtcgta ttatcaggac tgctggcttt tatcgtcttg tataatctaa ctaatatcaa
     3181 tatttccgag cgtgtacgcg aattatctac aataaaagtc cttggatttt ttgataaaga
     3241 agtcacgatg tacattgttc gagaaaatat catctttact cttttaggaa tcatcggagg
     3301 atttggtgta ggatatatat tgactgactt catcttgcaa caagcgtcta tggagaatgt
     3361 catctttcca ttagtgatca catggtgggc ttatgtctta tcagcaggat taactatcgt
     3421 atttacggtt attgtgatga ttgttaccca tttcaaatta aagcatatcg atatgattga
     3481 tgcgttgaaa tcgaatgaat aaaaacagag gttgtgaaat cagcggtttg acctgagtat
     3541 tagagaaaca aacggcaaaa tagtttttca tattttgccg ttttttgagc taataccgaa
     3601 ggacagctga ttgaacacca tgaatcagag gttgtaaaaa agccgtcctg catcgagcaa
     3661 aaagaatact caaataaaat tagctcctca tgttttacat ttgagaagac tgattgccgt
     3721 agataaaagc ttttgaacac catttataga agaaacaaga atattttgct attttaaaca
     3781 agaggataga caaatacatg tctgatctct tgttttttat tgggcaacag acctcttaga
     3841 atgtataaag ataaatttta caaacgcttt cggttcagtt ataataataa atgataactt
     3901 gtatatataa gttgtggtag gtaataaatc agttgaatgg aggtaaatat ccatgacatt
     3961 taaagacaaa gtcatttacc aaatctatcc caaatcttac aaagacagta atggtgatgg
     4021 aattggagat ttaaaaggaa tcaaagaaaa actcccgtat ctgaatgatc taggcatcga
     4081 catgatttgg ctaaacccta tttatccaag ccctcaaaaa gacaacggtt atgatatctc
     4141 tgattacaca gccatcgatc ctatctttgg tacgatggag gaattcgaac agctaatagc
     4201 cgaagcaaag aaataccgga tagaaatcat gctcgatatg gtgctgaatc acgtttcgac
     4261 agaacatgaa tggttcaaaa aagcactagc aggggaccag cattatcagg acttcttcat
     4321 tttaagagaa gagccaacgg attgggtatc aaaatttggt ggaagtgctt ggtcgccatt
     4381 tggagatacg gggaaatatt atttgcactt atatgatcgg acacaagctg acttgaactg
     4441 gcggaaccaa gcagtaagag aggaactatt caaagttgtt cgtttttgga tggaaaaagg
     4501 ggtaaaagga ttccgtttcg atgttatcaa tgttatcggt aaagacgaac aattgaaaaa
     4561 taataaagaa aatgatggca aacctgaata tacggacaaa ccaatcaccc atacgtatct
     4621 aaaagaattg aaccaagcaa cgtttgggac ggattccact atcatcacag tcggtgaaat
     4681 gagctctacg acgatcgaaa attgtatctt gtatacagaa cctagtagag aagaattatc
     4741 aatggtcttt aatttccatc atttgaaagt cgattatgaa aatgggaata aatggtctac
     4801 tcctccattt gattttgcag aattaaagcg actatttcat acatggggag aaaagatgag
     4861 tgagggtaac ggctggaatg ctctattttt taataatcat gatcagccac gtgcgctcaa
     4921 tcgatttgta gctatcgatc agtatcgaaa acaaggagca gagatgctag caaccatgat
     4981 ccatttgaac cgtgggacgc cctatattta tatgggagaa gaaatcggca tgatcgaccc
     5041 agactattca tcgatttccg actatattga cgtagaaagt ttgaatgctt ataagttact
     5101 ccttgaacaa ggattcacag aaaaagaagc gtttcgtcgg atcaaagcaa aatctcgcga
     5161 taactctcga acacccatgc aatggtcaga cacagaacaa gcaggattta ctacagggaa
     5221 accttggtta aaagttggag gcaagctaga agagattaat gtagaaaaag aactatcttc
     5281 tgaagattct attttgtcct attacaagaa attgattcgg ctgagaaaaa catatccaat
     5341 cgttgcacaa ggagattatc atgcttatga agcaaatcat ccggaagttt atggctttct
     5401 tcgccaattt gaaggacagc agctgcttgt attaacaaat ttctatgata aagatacaag
     5461 gatcgccatc ccagaaggat tcacagaagc caatattctt atcgataatt attccgatac
     5521 tgttcttcat caaagtctgg agctaaaacc ataccaggca attgctttgc tgaaaaatta
     5581 aaacaaaaac acaaaacgtc gttcctacga gctattctcg taagatcgac gttttgtgtt
     5641 ttatttaaaa taatcttctt cagaaaatga agagttttca tactctcctt tatcgccata
     5701 aaatttattg tagcgttgtt tgaatttacg gaaaataaaa gtaacgagta atttgactaa
     5761 cgtatagata ggaataccaa aaataacgcc cacaattccc attagatcac ccatgacaag
     5821 taaaacgatt aagatcgtaa tcggatggat ctgtaaacga tcacccatta ctcgaggaat
     5881 caccaagtca ccgtggagaa gttgtacggc aaaccagaca atgacaaatt tgacagccat
     5941 aaaagtagag tcttggaatg caatgatcaa acctggaaag aaggcgataa acggcccaat
     6001 atatggaatg atacataaca acccagcaga aatagctaaa acactagcaa attctaatcc
     6061 gatcagtaag aaacaaaccc agtagactgc tccaaggata aatgaagcaa tgatttgccc
     6121 ctttagaaat gaacccaatt gttgattagc aatctttgtc aaatttttaa aatctgtacg
     6181 aaatgctggt gggacaatcg ctaaaactga ctgataaaat ttttgaggat ctctcaaaag
     6241 gaaaaaagcg acaattggac cagtaaataa agtaataaaa atagctgtaa cagttgaaaa
     6301 aatgttgctg atccgttgtg cgccactttc ccaataattt ccaataaatc cagctatatc
     6361 agacgtcacg tcgtcaatag aagcgaaaaa ttgattaaat gaatcggcaa aaggtgtttt
     6421 ctccaaaaat tgtcggagac tctcttgaaa atcttgaaac aaatctggca aactactgat
     6481 caatgtcttt gcctgttctt caagaaaagg aataagccat atgcttccaa gccccaataa
     6541 aaataaaata atcacataaa cgataatgac tgacaagttc ctcgaaaggt gcttgctcaa
     6601 acgtttgatg actggttcca aaagataata caagatcagt gcaaacaata atgggggcaa
     6661 gatagtagcg ataatgattc cgacaggctt gaatataaac gagatctgat ggaaaaaata
     6721 aatgatcagc gcaagtaaga taactattcc taaaacatag taagaggttt ttcctcctaa
     6781 aaagcggatc aaacgattcg ttgtatctgt ttcttttttc actctttttc ctcctttatc
     6841 tataacttca ttatagacaa aagcgggtat aaaaggaaaa tgatagaggt tgattgcaaa
     6901 aatatcgtca atcattcata taaactttct catcaattaa aataagaaaa cgcttgacag
     6961 ttcttttaac catatgataa attaaatcct gttgctaaaa cgtaactatt acggtttgcg
     7021 aaaagcgaat cggaacattc aaatgaaaga agatgagaag aggagtgtca taaatgaaac
     7081 gattgatagg tatattggcc atgctggttt tagcaggtat gttaactgct tgcggggcaa
     7141 gtgggaaagc cgaggacagt aaagaaaaat tgagcgtgat gacgaccttt tatcccatgt
     7201 atgattttac aaaagcaatc gtcggggatg aaggagaagt agagttattg attccagcag
     7261 ggactgattc ccacgattat gaaccttctg ctaaagacat ggcaaagatc caagatacag
     7321 atatctttgt ttataatgat gaaaacatgg aaacatgggt ccccgcaatc caaaaaacat
     7381 tacaagaagg caatgtccat acgataaaag cgacagaagg tatgctttta cttccgggaa
     7441 gtgaagaagg acatgatcat gaccatgaac acggggaaga aggccataca catgagctag
     7501 acccacatgt atggctagct ccaagtttag caatcaagca ggtagcaaac atacgtgatc
     7561 agctgattga agcttaccca gaaaaacagg aagtatggac aaaaaatgca gcagcctata
     7621 ccgagaaact tcaagcactc catcagcttt atcaagaaac ctttaagcaa gcaaaacaaa
     7681 gatcttttgt cacgcaacat gctgcattca actatctagc gttagaatat ggattgaatc
     7741 aagtatcgat tgctggtctt tcttctagtg aagaaccgtc tgcggcgaga attgctgaac
     7801 tgaaacactt tgttaaagaa catggaatca actatattta ttttgaagaa aacgccaaag
     7861 atagtattgc cagaacgtta gcaaacgaag caggtgtatc tttagaagta ttgaatcctt
     7921 tggaaggatt gacgaatgaa caaatagaaa atggagaaaa ctatctttct atcatggaag
     7981 caaacctcga agcgttgaaa aaaacgacag agacagaaaa cccgctagaa gatactttga
     8041 cccccaaaaa agaaaagagc gtctacaacg gttattttga agacgaggat gtacaagatc
     8101 gctctctttc agattgggca ggaacttggc aaacagtaga tacgtatgta gaagatggga
     8161 cttttgatcc tgtttttgaa tataaagaaa aattgaatca ggataagaca gcacaagaat
     8221 ataaagaata ttatacgaag ggctatcaaa cagatattgc ttcaatcaag atcagtgaga
     8281 aagagatggc atttacgttt aagaatggaa caacgaaacg ttcaaattat cgttatgtag
     8341 ggaaagaaat cttgacctac gaggcaggaa accgtggcgt aaggtatcta ttcgaagcag
     8401 aagataagga aagtggcgct tttagatata ttcaattcag tgatcacacg attgcaccgg
     8461 ctgactccga acattttcat ttatatgcag gaa
//
LOCUS       DAZBSA010000108         8465 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000108 DAZBSA010000000
VERSION     DAZBSA010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.109"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            7..2199
                     /locus_tag="ACHGMT_002406"
     CDS             7..2199
                     /locus_tag="ACHGMT_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFN6836120.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            2216..2692
                     /gene="ybeY"
                     /locus_tag="ACHGMT_002407"
     CDS             2216..2692
                     /gene="ybeY"
                     /locus_tag="ACHGMT_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFN6836121.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            2676..3071
                     /locus_tag="ACHGMT_002408"
     CDS             2676..3071
                     /locus_tag="ACHGMT_002408"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFN6836122.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            3086..3985
                     /gene="era"
                     /locus_tag="ACHGMT_002409"
     CDS             3086..3985
                     /gene="era"
                     /locus_tag="ACHGMT_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFN6836123.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            4128..4940
                     /gene="recO"
                     /locus_tag="ACHGMT_002410"
     CDS             4128..4940
                     /gene="recO"
                     /locus_tag="ACHGMT_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFN6836124.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            5324..6241
                     /gene="glyQ"
                     /locus_tag="ACHGMT_002411"
     CDS             5324..6241
                     /gene="glyQ"
                     /locus_tag="ACHGMT_002411"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFN6836125.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            6243..8318
                     /gene="glyS"
                     /locus_tag="ACHGMT_002412"
     CDS             6243..8318
                     /gene="glyS"
                     /locus_tag="ACHGMT_002412"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFN6836126.1"
                     /translation="MAKDLLLEIGLEEIPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFTHAKQYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT         2861 a         1532 c         1804 g         2268 t
ORIGIN      
        1 aagcacatgt tgaataaaac actgcagaaa ctacaagaaa aacttggaaa ggcagcattg
       61 cctttcctgc tgttcttgtt ttttatcatt atctgcggcg ttacatttag tagtgtcaaa
      121 caaaaggcca atgatttcag agaaggccaa gtagcagaag aaagtatccg tgcgaacaaa
      181 acgatcgaga acacggaaga aaccgaacaa aaaagaaaac tggcagcaga agcagtcact
      241 ccagaataca cttaccaaaa agatcttgca gatgaccaga acaatcggat caagcaacta
      301 tttgaattga tcgataagac gaatgatgcc atcaacaaat cttatgacga aaagattgat
      361 aaggcaaagg aagatgaaaa tatcccaaag cctactacag aagagcggat cgccgcgctg
      421 aagaaaaatt ttgaaaatgt gaatgcagaa aatgttgtgt tttatcaaaa actaccgaac
      481 aatttttatt cgacgatatt tgaaatgact gaaactgaga ttcatactgt gagggatgag
      541 agtctgaagc tcattgacga acagatgagc aaacaagttc gtgaaagtga attggaagct
      601 tttaaacaag aagcagaaga gcaaatccaa tatttgaatg tcacaccagc acaacaacaa
      661 atgattcgct acttagtgga tcaaggtatc gtagtgaatg acgtattgaa tgagaaaaag
      721 acagaagaat tgaaacaatc cgcacgagaa gctgtacaac cagtgatgat cttccaagga
      781 gaaatcattg ttcgtgaagg taatcaaatc gatgcttcag ctatgaaaaa attggaatta
      841 ctaggactga ctaatcaaac aacttcgatt tttccgctaa ttgccatggt tttagctgtt
      901 ttgcttcaaa tcggcgtact gatttataat tcacttcaat tccaaaatgc aggagaaaga
      961 acaaaatacg tgcttttcta tgtgacagct atgtcagtca gcatagtgct gatgaaattt
     1021 ttccaattat tccaaacgga acaagctact tatatcccgc tgttttatcc cgctgctttt
     1081 gcaccacttg ttttaagctt tttcttgaat cgaagagcag ggatcatggc tggtattttc
     1141 caagcagtat ccgcactttt cattttttat gattcgattg gcacgaactt tctaacgatt
     1201 atcttgatgt cttatgtttt ctctgggctc atggcaacga ttgtcagacg caaacggatc
     1261 tcagaacaag gattatctgc tgccatgtgg gtcattattt ttcctgtgat gatggatgtc
     1321 attttgatcg tctatcaagg aatgagcttt ggtgatgcaa cgacttggtt aatgctcgtc
     1381 tgcgggttca ctggtgcact cttctcattc ttgctgacga tcggtctaca cccatatatt
     1441 gaattgatgg tcaatgatga cagtgtaatt gtattaaatg aactcagcaa tccaaatcat
     1501 cctttattga aacagttgct ggaagaagcg ccaggtactt atcatcatag tatgatggta
     1561 gctaatctaa gtgctaacgc tgtagcagaa atcggcggac gttctttgct aactcgagta
     1621 gcttgctatt atcatgatat cggcaagatc aaacatgcta gctttttcgt tgaaaacttg
     1681 ccttcaggag cggaaaaccc gcacaatttc ttattgccag aagacagtaa acatattatc
     1741 tttggacatg tgacagatgg agccaaaata ttggaagatt ataatatgcc gcaaatggtg
     1801 atcgatattt gcaaacagca tcatggaacc acgctaatgc gttatttcta catcaaagcg
     1861 aaagaacgca atccggaagt aacagaagag caattccgtt atcctggacc aaaaccgcaa
     1921 agtcgtgaag cagggatcgt caatattgct gacagttgtg aggctgcagt acgggcaatg
     1981 gatcatccga cgagcgaaaa aatcgaaagt tttgtccata atcttatcga agagcgtatt
     2041 tccgatggtc aattagacga tagtggactg accttgaaag aaatccgaaa agtggaaaaa
     2101 tcattaatca gcggtttaag tagcaccttc cattctcgga tcaaatatcc taaaatgaaa
     2161 acagaagttg agaagataaa ggaagaacaa gaagaataga aagaagcgat gtataatgga
     2221 tattaccttt atggatgaaa caaaccaagt ctcagaagag aaaatgaaag aaatcgatga
     2281 cttgctgcaa tttgcagcat cttatttaaa gctgccagag gaaacagaaa tgtctgttac
     2341 tttcatggac aatgcggcaa tccaagtaat caatcgtgat taccgtggaa aagatgcacc
     2401 gacagacgtc atcagttttg ctctggaaga agaaggcgaa gacgaaatcc cagtgatttt
     2461 tgaagatgga gagaatccat tacctcgtgt actaggagac atcatgatct ctattgaacg
     2521 tgctgctgaa caagcagaag cttacggcca ttcatatgat cgggaacttg gctttttagc
     2581 ggtacatgga ttcttgcata tcaacgggta tgaccatatg acaccagaag atgagaaaga
     2641 aatgtttggt ttacagaaag agatcttgga tgcctatgga ctcaaaagat aaaaacgtag
     2701 agaaaaacaa acatttcctg atgtctgtgg aatttgctgt ccaaggagtc aaaaccgttt
     2761 ttgaggaaga acgcaatatg aggaaacatg ttgcgttcgg tatccttgct ttatttatgg
     2821 gcgtgatttt ccagcttgat cggatagagt ggttatggct gcttttagct gtttttctcg
     2881 tttggatagt agagatcatc aatactgttt ttgaaaatgt agtggacatg tttacggatt
     2941 ttcattttca tccgattggg aaaaaaatca aagatatggc agctggagca gttttagtga
     3001 ccgcttgttt tgctgtcatc attgggttga ttttatttgt ccctaaaata tatcatttgt
     3061 tgtttaatta aaaaggagaa cgtatatgcc agaacataaa tcagggtttg tcgcaatcgt
     3121 cggccgcccg aatgtaggaa aatccacatt attgaaccga atcgtgggtc aaaaaattgc
     3181 cattatgagt gacaaggcac agacaactag aaataaaatc caaggagtat atactacacc
     3241 agaagctcag ctgattttta tcgatacacc aggaatccat aaaccaaagc atcgtctagg
     3301 agattttatg gtagaagcag cttacagtgc gttgaaagaa gtggatgcag tcttatttat
     3361 gatcagtgct gatcaaaaac gtggccgggg agacgatttt attattgaac gtctaaaaaa
     3421 cgtgcattct cctgtttatc tgattatcaa taaaatcgat aagatccatc cagacgagtt
     3481 gctgggaatc atcgaagatt attcgacgca gatgcctttt gcacaagtga ttccgatctc
     3541 tgctacagaa ggaaacaatg tcgaacgctt aatggatgta ttagtcagtg aaatgccaga
     3601 gggcccacaa tattttccag atgatcaagt gactgatcat cctgaatact tcatcgtttc
     3661 tgaattgatc cgtgagaaag tcttgttttt aacacgagac gaagtgcctc attctgtcgc
     3721 agtcgttgtt gattcgatga agcgaaacga gaatgataaa atccatatcc aggccacgat
     3781 catcgtcgaa agagatagcc aaaaaggaat cattatcggc aaaggcggaa aaatgctgaa
     3841 gcaagtagga acgaaagcaa gaaaggatat cgaaaatctt ttaggagaca aagtatttct
     3901 agaactttgg gtcaaagtcc aaaaagactg gcgagacaaa aaagtctatc tgcaagactt
     3961 cggctaccga aaagacgagt actgatattc tggacttctt ataggttagg tatagaaata
     4021 aactggataa aaatccgtac gttgagaaaa acagcggaag atcgaaacat aaataatctg
     4081 gatcggcaga atctgctttt ccagatttat tttttaggag ggagaatatg agccagcttg
     4141 cagaatcgaa aggcttgatt ttatctagcc gtaattacaa agaaaaagat aaattagtca
     4201 aaatctttac ggaatcttct ggcaaaatga tgttttatat caaaggaatc catcggcaaa
     4261 atcagccatt agctccggct ttgctcccct ttaccgaagc agtctatatc ggtaattttc
     4321 gaagtgaagg tttgtcgttt ttaagcagtg tcaaagacgt ccatgcgttt cggaacatcc
     4381 aagaagatat ctttttgagc ggttatggta cgtatttgct aaatctagta gatgcagcaa
     4441 tcgaagatca tcaatatgac ccgcatttat tccaatttac gcaacaagct ctgcagcgga
     4501 tggaccaagg agatgatgca gaaatcatca cgaatatctt tgaagtgcaa atactgcaac
     4561 gcttcggaat cgcgccaatt tggacgcact gtgtcgtatg cggggaaaca aaaggaaaat
     4621 ttgactattc ttccaaatat ggcggtgtga tatgcgagaa acactggcca atggacgaac
     4681 accgttatca tgcagatcct cgcgctgttt atttcgttcg aatgttttcg gctatttctt
     4741 atgataagat cagtggaatc cacttgaaag aagaaacgaa gcaggcaatc cgtgacctca
     4801 ttgaccagtt gtatgatgaa tatgtcggta ttcatctaaa aagcaaaaag ttcattgatc
     4861 aaatgaagtc ttgggcacac gtattaaaga cgccagagaa aaatatgcct gaaaaagaga
     4921 tacctgaaaa agaagaataa atgaaagata caacaaaaaa atcaaaacta tttgacaaat
     4981 acagggactt ccgtctatta ttaaccgtag attaaataga tcgttcactg agaggatcta
     5041 ttatacaaac cagaaaactg caaaatgaat acgtacattg accaagatca gtaaaaaaca
     5101 cgtggtttag cgagttcggg agagtggaag ccggacccaa acgatttttg aagaggcact
     5161 tgcgagtacg ataaatgaag ctgccgaatc tttattcgga agtagggtgg aaccgcgaaa
     5221 ttattcgtcc ctatgtctgt aatcagacat agggactttt tgtcgttttc tttcctgtat
     5281 tcttattgac agcaacgctt aaaaattgaa aggggcaaaa cagatgagta aaaaattgac
     5341 cgtccaagat atgattttaa cattacaaaa attttggtca tcaaacggct gcatgctgat
     5401 gcaagcttat gatacagaaa aaggagcagg tacgatgagc ccatatacat tcttacgggc
     5461 aatcggaccg gaaccatgga atgctgctta tgtagagcca tcccgccgac cagctgacgg
     5521 gcgttatgga gaaaacccaa accgtttgta tcagcatcat caatttcaag tcgtaatgaa
     5581 accttcacct gaaaatatcc aagagcttta tttagaaagc ttgcgtcttt taggaattga
     5641 tccattagaa cacgatattc gctttgttga agacaactgg gagaacccat cgatgggttg
     5701 tgccggtgtg ggctgggaag tatggcttga tgggatggag atcacacaat tcacatactt
     5761 ccagcaagtc ggaggactac cttgtaaacc agttacttct gagatcactt atggactaga
     5821 acgtcttgct tcttatatcc aagaagttga aagtgtttat gatctggaat gggctgatgg
     5881 cgtgaaatat ggtgagatct tcaaacaacc agaatatgaa cattctaaat attcttttga
     5941 agtaagcgac caagacctgc tactaagcaa ttttgatcgt tttgaaaaag aagctaaacg
     6001 ctgtatcgat gaaaatcttg ttcatccagc gtatgattat attttgaaat gtagccatac
     6061 attcaattta ttagatgctc gcggtgcagt atccgtaaca gagcgtgcag gttatctttc
     6121 ccgtatccgc aacatggctc gtgctgtagc caaaatcttt gtggcagaac gcgaaaaatt
     6181 aggattccct ttattgaata aaaacacagc aaatccagaa aacgtgaagg aggctgaata
     6241 agatggcaaa agacttatta cttgaaatcg ggctggaaga aatacctgct catgtagtca
     6301 caccaagcat gaaacaatta gaagaaaaaa cagcaaaatt tttagatgaa aaccaattga
     6361 cttatgatac gatcgaaacg ttttctacac cgcgccgctt agcaatcaaa gtaacaaaca
     6421 ttccagaacg tcaagcagac agcgaagaag aagtcaaagg accagccaaa aaaattgctt
     6481 tggacgcaga tggaaactgg acgaaagcag ctgaaggatt tgtccgcgga caaggtctga
     6541 cgacagatgc tatcactttt agagaattaa aaggcgtgga atatgtctat gtaacgaaat
     6601 acactcacgg caaagaagca aaagaagtat tgacaggttt agcagatgtc attaaaagcc
     6661 tcactttccc agtaacgatg cactgggcag aatacgattt tgaatatatt cgccctattc
     6721 actggatcgt ggcattgtta gatgacgaaa taatcccgtt tgagatcttg gatgtcaaaa
     6781 cagacagagc ttcaagaggt catcgtttct taggcgaaga tgtgacattt acgcatgcaa
     6841 aacaatacga agaaaaattg aatgaacaat ttgttattgc ttcaccgcaa aaaagaaaag
     6901 aaatgatcat cacacaagca aacgaattag cagaaaaaaa tcactggatc attgatctag
     6961 atgaagattt actagaagaa gtcaacaatt tagtcgaata tccaactgtc ttttctggcg
     7021 acttcgaaga aaaatatcta tctgttcctg aagaagtact agtcacttct atgaaagaac
     7081 atcaacgtta tttcgaagta cggaacgatc aaggtatgtt gcttcctcat tttatttctg
     7141 ctcgtaatgg cgatcgggta aaattggaaa atgtggtacg aggcaatcaa aaagtattga
     7201 ctgctcgttt agaagatgcg gaattctttt ataatgaaga caagaaagct tcaatcgacc
     7261 aatacgttga aaaattaaaa actgttactt tccatgaaaa aatcggtagc atctatgaga
     7321 aaatgcaacg ggtacaagtc atcgctcgca tcataggcga acaagtagga ctatctgagg
     7381 aagaattgat ggatctgcaa cgcgctgctg aaatctataa atttgatcta gtaaccaaca
     7441 tggtaggaga attccctgaa ctacaaggga tcatgggcga aaaatatgct ctgcttaaag
     7501 gcgagaaacc agccgtagca acagcgatcc gtgaacatta catgcctata tccagtgaag
     7561 gagatttacc tgagactaca gtaggttctg ttttagcgat tgctgataaa ttagatagta
     7621 tcttttcttt ctttgcagtt gggatgatcc caagtggctc aaacgatcca tatgctttgc
     7681 gccgtcaagc atatggtgtc gttcgaatca tcgaatcgaa aaaatgggcc tttcctttgt
     7741 ctgttctgca agaaacgatt agtgaagtta tcagtaaaga tacggatcgt tttggcattg
     7801 gcttgtcagc gggacaacaa caggtgatcg actttatcaa aggccgtctg cgccaattat
     7861 tgacaacgaa gaatatccgt catgatgtca tcgaagctgt tttgaatgca gaacaaaaag
     7921 atctgacaaa agtatttgca gcagcacaat tgttcaagca gcatcttgca gatgaagact
     7981 tcaaaccatc gatggaagct ttgacacgtg tcgtgaactt agcgaaaaaa gcggagcttg
     8041 aaaaacaatc agaagtagac cctgaactgt tcgaaaacga agcggagaaa gaactgcaca
     8101 aagcagtaga ggcaatgaaa acaagctttg ctgaccaaac gatcaacgaa aattatgatc
     8161 agcttgtaag ccttcgccca ctgatcgaaa attattttga tcagacgatg gtcatggcag
     8221 aagaaccagc agtacgtaag aaccgattaa cgcaattaag tcaaattgct gccatggctt
     8281 tgtcactagc aagtttagat caaatcatca caaaataatt tctaatatgt aaataaacca
     8341 gagaacaggc acaaaatagg ctatttctct ggtttatttg ttataaatat aaataactat
     8401 tattaattca atgttaattt ttttgttatt ttattttttg tgtaactttt aaaaacacct
     8461 atgaa
//
LOCUS       DAZBSA010000109         8231 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000109 DAZBSA010000000
VERSION     DAZBSA010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.110"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            331..1254
                     /gene="truB"
                     /locus_tag="ACHGMT_002413"
     CDS             331..1254
                     /gene="truB"
                     /locus_tag="ACHGMT_002413"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFN6836127.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1258..2199
                     /gene="ribF"
                     /locus_tag="ACHGMT_002414"
     CDS             1258..2199
                     /gene="ribF"
                     /locus_tag="ACHGMT_002414"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFN6836128.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2594..3379
                     /locus_tag="ACHGMT_002415"
     CDS             2594..3379
                     /locus_tag="ACHGMT_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN6836129.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3392..4243
                     /locus_tag="ACHGMT_002416"
     CDS             3392..4243
                     /locus_tag="ACHGMT_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFN6836130.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4367..4819)
                     /locus_tag="ACHGMT_002417"
     CDS             complement(4367..4819)
                     /locus_tag="ACHGMT_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6836131.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5284..5652)
                     /locus_tag="ACHGMT_002418"
     CDS             complement(5284..5652)
                     /locus_tag="ACHGMT_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFN6836132.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5802..6284
                     /locus_tag="ACHGMT_002419"
     CDS             5802..6284
                     /locus_tag="ACHGMT_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFN6836133.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6321..7331
                     /locus_tag="ACHGMT_002420"
     CDS             6321..7331
                     /locus_tag="ACHGMT_002420"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFN6836134.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7446..8159)
                     /locus_tag="ACHGMT_002421"
     CDS             complement(7446..8159)
                     /locus_tag="ACHGMT_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836135.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2785 a         1337 c         1668 g         2441 t
ORIGIN      
        1 tgattaagaa actgaaaaaa taaataaaaa aaaacggaaa aaagactgga agaatggaca
       61 gtcttttttt tcgtttttgg gaagtgaaaa ccaaactatt tacctgtaag ccacccataa
      121 atccaatcaa atggtgcaaa gaaaaaatct tccattctat tccctccttt aaaaaaaaca
      181 taacatgatt tctatttacg tgagcatgaa ttttgttacg tttttattgc cgaaaaataa
      241 aaaccgttca ttatgatgtc aaattcgtca gtaattgatg caatgtgtta taataattca
      301 gttaaatcaa aaatgaaatg gagaaaagac atggacgggt tattaacttt atggaaagaa
      361 cgcggaatga caagtcatga ttgcgtattc aaattaagaa aaatcttaca tacgaaaaaa
      421 atcggacatg ggggtacgct tgacccagac gtagacggtg tgttgcccat ttgtattgga
      481 aaagccacaa aagttatcga atatttgaca gattctggga aaatctatgt aggcgagata
      541 actctaggtt tcagtacaac gacggaagat cgatccggag aacgggtaga aacaaaacca
      601 gtagatatgt ctttatctga agaaaaaata gatgaagtga tggcttcatt tattggtgag
      661 atcacccaga taccaccaat gtactcagcg gtaaaagtca acggtaagcg gttatatgaa
      721 tatgcccgca ataatgaaac agtggagcgt cctgttagaa aggctcagat ttaccgtttt
      781 atccggacaa gtgaattgac aagagacgaa gtgacaggaa ccatttcttg gcgcttcgaa
      841 gtagaatgcg ggaaaggaac ctatgtccgc acattagctg tagatacagg caagaaactt
      901 ggttaccctg cgcacatgtc tgatctgacc cgaacagcaa gtgccggtat gtcccagaaa
      961 gatgctctga cattagaaga agtagctcaa gctgtgaaaa atgggacaat cgatgattgt
     1021 ttgctgccaa tagaaaaagg agtgaaacga ttcaaacgag tagatattac tgatgaagta
     1081 tggcagaaag taaaaaacgg tatgagactt aattatactg tatttgcgct cccagaaatg
     1141 ccgtcagaag aaattgcttt gttttataaa ggcaaagttg tcagtatcta tcagccaaac
     1201 ccgaaagaaa aaaacaaatt aaaacctagt aaagtattga gaaatgagga ataacgaatg
     1261 aatatcatca agatcagaca tccttaccaa tcagaacaaa ttccagaaga cgatgtcgtt
     1321 ttagtactag gattttttga cggcgtgcat attggacacc agaaagtcat aaagacaggt
     1381 atggaaattg cacaaaaaga aggattgaaa ttagcattga tgactttcaa tcagcatcca
     1441 tcgattgtct ttaaaaagat cgatcctagc agcgtgaaat atttgactag ccttaagcaa
     1501 aaagaagaaa aaatggccgc tttgggtata gattatttat atgaagttga ttttacttct
     1561 tcttttgctc atttagcacc gcaggaattt gttgatcaat acatcgtagg gttacatgca
     1621 aaatatgctg tttccggttt tgattatacg tatggaccaa aagacattgc agatgtcgct
     1681 cattttcctg aatatgccaa aggaagattc aaaatcgtca cagtttctaa agaagaagaa
     1741 caaggtgaaa aaatcagctc tacccgtatt cgaggattac tagacagcgg acaagtaaaa
     1801 gaagcagcaa aactattagg ttgcacatat gaggtagaag gggtagttgt ccatggcgat
     1861 gcacgaggca gactactagg attccctaca gcaaatgtca aagtaaagag cacagttcat
     1921 ttgcctaaag aaggtgttta tgtatcggaa ataaaaattg gggaaatttg gtatccagcc
     1981 atgggttcaa ttggacataa tgatacattc ggtgaaggac gggagctgac tgtagaattg
     2041 tatattttgg actttaatca agatatctat ggtgaacatg tcgcagtacg ctggaatcaa
     2101 tttttaagag atcaagtgaa attttctagt gtagaggaat tgattgaaca actcaaagag
     2161 gatgaacaag caacagctga atatttttct cagcaataaa acattatgca taaaatctct
     2221 tgtttatgta taactagcaa tttcaataaa atgggtgata aataagccca ggaaaggggc
     2281 tgtgacaaaa gtcttgtcac agcctttttt tccaaataaa tggtttttca gaagtagctt
     2341 cttcggaaat aagccgaaat ttcacaaaaa tttgagaagt aatttttgta aattacttct
     2401 tatttcttga agctggccac ttctgtcaca acctctttct ttatagaaat aaaaggacac
     2461 tagcaattca tgaaaacgat tgctaaagcg tatcatttgc tttagactat atatcttggt
     2521 gtacactcac tttaagacgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa
     2581 aggagtgggt actatgtata aggaattaga aaacaaagtc gcagtagtaa ctggtggctc
     2641 aaaggggatt ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt
     2701 caactataac tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg
     2761 cggaaaagca gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact
     2821 tgatacggct ttagaaaatt ttgggacact ggacgtttgg attaataatg ctgggatgga
     2881 aaatcagcat ccaacccatg aacttagcct tgaagattgg gaaagagtat taaaagtcaa
     2941 tttgactggc gtttttatcg gaacgaaagc ggctttaaat tactttacag agaatgataa
     3001 aaaaggaaat atcatcaata tgtcttctgt tcatgaacaa atcccatggc caacgtttgc
     3061 tcattacgct gcttcaaaag gcggagtaaa actattcacc gaaacggtag caatggagta
     3121 cgcaccaaag ggaatcagaa tcaatagtat aggacctggt gctatccgta cacctattaa
     3181 tgcggcaaaa tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg
     3241 acgcgtaggt gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc
     3301 cagttatgtt acaggtatta cactatttgc agatggtggg atgactttat atccttcttt
     3361 ccaaggtgga aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc
     3421 agttgggtgg gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga
     3481 gatattagga actggtattg gagccgtgct cgttggctta cttgttcaat tattcgcttc
     3541 tccagctggt atttcagtga ctacattttt gattagttta ctttctggtg cattctgggt
     3601 catcggacaa acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc
     3661 aatttcgact gcattacagt tagtcggaac ctcattgatt ggtgtcttcg cttttggaga
     3721 atggtctgga agtatgggaa aaatcattgg tgcagcagca atcattcttc ttgtatttgg
     3781 ttctgcttta acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat
     3841 cagtctatta gcatcaacaa gtattggtta ttgggtttac agtgcattgc ccaaactagt
     3901 tgatgcaagc ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt
     3961 gatttatgtg attttccgaa aaccaaccgc attttctgaa gacaagagtt ggaaagcaac
     4021 aatagttgga gccatcttca gtgtgtcagc attggcttat attttttcag caaatgaaaa
     4081 tggggttgct actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg
     4141 tatgtacatc ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt
     4201 attgctgatc gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa
     4261 tctcatttat taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc
     4321 gctagaattt ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaagtg
     4381 aaatatctgt ctgcatcaat aatttttcat tgatccattg actaaaattt acgacatgat
     4441 tgaagttacc accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa
     4501 gacggtcttt tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggattt
     4561 gattgattgc tgtaaaatca cgatactgaa ggttgaaacg catcgtgatt ggtgcattta
     4621 gcatgaatcg ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa
     4681 aaagattacg ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt
     4741 catgaagata atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta
     4801 ctgagtaaaa atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga
     4861 tatttgctta atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca
     4921 agtcagaaaa ttctaacttt tctttctcta aagaaattcg ttctctccta aagtgatatt
     4981 atatttaaat atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg
     5041 tattttttcc ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactaagt
     5101 ggaaaattca aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa
     5161 aatagcagat actttgtcaa ttaagtgttt aaccagggat gataaaaagg ataggattga
     5221 acaaaacctg tataagctga tgaatagttt cctcgccagt aaaaggttta ttcgtataaa
     5281 gctttatcgc caaaatttat catgaccttt tttatcttca atattaaatg aaatcaattg
     5341 ttcaatcaat tcccagttga ttacactttt ccaaggaact ttgatgacgt tatccgtata
     5401 tacgtagcct gcttcttgga tatttttttc aagggcacga atcgctgctt tttccggtgc
     5461 gatagaaaaa tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa
     5521 cataggttgg ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc
     5581 aaaaaggtct ttgatctttt gttgatgttc tttttctttg atctgattga tgatcggctg
     5641 aaatttttcc attctaaaac gcctccatta agtggtttga tttattttac cagaaaaaca
     5701 atagttatgt ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta tatttatatg
     5761 ataagatgtc tcaaataata aaatagaaaa gagacataaa aatgattgac ttggcaaaga
     5821 aattcttaca cgaaaatata tattttgagt tgtatcatca tcgagccatt tatacaaatg
     5881 aagatgcttt ggtaataaag aaagaacaag gatttacagg tacagagacc aaaagcttat
     5941 tcttaaaaga taaaaaagat aataactacg tatatctgac ttttactacg aaacaaacgg
     6001 atttcaaagc attagggaag atcgtcggta aaaggctttc tattgtttca tcagaacgca
     6061 tggaagaaca aacgggacaa aagcagggcg ctgtttctcc gtttggttat gaaaaacaga
     6121 ttccgattat tattgatgaa gagctcttat caaatgaaaa acttgttttt gcaccaggta
     6181 gaccagatca aacgatggtc atccaaacgg ttgacttaaa caaaatcatc cagctacttg
     6241 agatgccgat atttttgatg ccggcaaacg aaaaaaacaa ttaaccaagt aaaatagtgc
     6301 atacttggag gtgaagcaac atgataagtt atccattatt agaaagaaat gcaacgattg
     6361 gggttactgc accatcctca ggcgtatcag aggaacacca ttcgttactc gaatcagctt
     6421 taaagagaat ggaagaaaaa tctttccaag tagttactgg aaagacgctt tggacgcaag
     6481 ataaggaaaa atcaggtcca gcaagaaaac gagcagatga attcaatcaa atgatgcaag
     6541 atgaaaatat ccaaatgatc tttccgcctt ggggaggaga attattgatt gagattctcg
     6601 attttgtcga ttttgatgct ctacaaccaa aatggatagt aggttattca gatattagta
     6661 cgttgctact tgctgtcaca ttaaaaacag ggatagccac tgcacatgga accaacctta
     6721 ttgatttaag aggagagtac ttggatgaaa cgacagcaat gtgggaaagt gtactgaaaa
     6781 caagagaagg agaatcgatt ctacagcaac cttctgaaaa gtatcaaaaa gagtggcccc
     6841 atgagcattc atcatcttct gttttccatc tcactgaacc aacaaattgg caatcagtca
     6901 gcggagatgc agtaaggatg aagggacgat tattaggtgg ctgtattgat acgatcagtc
     6961 atttaatcgg taccccttat ggagatgtcc aatcttttaa aagaaagtat aatgatggaa
     7021 agccgatctt gtggtttcta gaaaattgtg agctatcgtc agttgattta agaagatcac
     7081 ttgttcagat gaaactggca ggatggtttg atggcagctc ggggatcatg ttcggtagaa
     7141 gtgcagcaga ggatttgtta gattataaaa tgaaggatgt atatgaagat ctgtcaaagg
     7201 aattaaaact accgatcttg tatgatattg attgtggtca tgtaccgcca caactgacat
     7261 tgatcaatgg tgcctatgca gaagtagcgg tagaagatag aaaagcgact gttttgcaaa
     7321 cgtttattta aaaaattttt tcctgccttg gagtttatca aagaccaaca aatctaaata
     7381 gaaaagaaaa tgaaagaagc tgacagtatt ttgccagctt ctttttgtat gatctattag
     7441 aaattttaat tatccataac tatcacattg atcagctgtt tcacatcatt tggattacct
     7501 gtaggaatag ctactacaga tgcattatca ataatttctt gagttgtttc tctaatgaac
     7561 gctggataag atgtttgatc tgcaggcatt tgccaatgtg aaggaactgg ataactagtg
     7621 attgtcccgt catggttact tgaatacact aaaactccct ggtaactgta tttcccagtg
     7681 agcataacca catctttcgg atagactgca ctccctgcat cataaggggc gataggtgtt
     7741 cctgcaggat aatgaataac atgtagttcg aaatcacccg atgtatctct aaaagtatta
     7801 cctctaattg ctagccaaac acgtgcgtac tcaatttgta gatcagaata tcctgctaac
     7861 ggatctattg actgagaaga ctgtgaggaa gttgtgtcta ttgttgaaga agaaatattt
     7921 gtctcaactg tactactaga tgtagaagaa gtattgggct ctgagacttt actgcttgtt
     7981 gaagatgtat tcgtttcagt agaagctgat ttctgagtac ttgatttttc agctgaactg
     8041 gtttgtttga ttgaactttt gtctgtggtc gcagatggat cactattttc tttactagga
     8101 gacgtacatc ctacaaggaa taaagagacc aacaataaaa taaaacctaa ctttttcata
     8161 taaaatacct cattattttt ttaataccag tatagcataa tcctgaattt ttgaatccgt
     8221 aaaaccttgt c
//
LOCUS       DAZBSA010000110         8146 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000110 DAZBSA010000000
VERSION     DAZBSA010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.111"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            65..511
                     /locus_tag="ACHGMT_002422"
     CDS             65..511
                     /locus_tag="ACHGMT_002422"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFN6836136.1"
                     /translation="MKIKIYNQEENQTRHIRNLSNAKLEEEARKVSNYNKKMAAKYVN
                     EEEFCFSKTKIYKRNPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLSQG
                     GKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
     gene            854..1177
                     /gene="relB"
                     /locus_tag="ACHGMT_002423"
     CDS             854..1177
                     /gene="relB"
                     /locus_tag="ACHGMT_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFN6836137.1"
                     /translation="MRFFKRGNYRAMITVRVSDAKKEWLSYMAEFYGITLSELLKNYS
                     MEQLEDEYNRQTAEIAHKHWIRSGKTTVFMEESLMNLVGWNDLPFKIHARNSEAALIG
                     KYVYW"
     gene            1497..1724
                     /locus_tag="ACHGMT_002424"
     CDS             1497..1724
                     /locus_tag="ACHGMT_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFN6836138.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            1740..2906
                     /gene="hemW"
                     /locus_tag="ACHGMT_002425"
     CDS             1740..2906
                     /gene="hemW"
                     /locus_tag="ACHGMT_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFN6836139.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            3141..4184
                     /gene="hrcA"
                     /locus_tag="ACHGMT_002426"
     CDS             3141..4184
                     /gene="hrcA"
                     /locus_tag="ACHGMT_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFN6836140.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            4278..4841
                     /gene="grpE"
                     /locus_tag="ACHGMT_002427"
     CDS             4278..4841
                     /gene="grpE"
                     /locus_tag="ACHGMT_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFN6836141.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            4892..6721
                     /gene="dnaK"
                     /locus_tag="ACHGMT_002428"
     CDS             4892..6721
                     /gene="dnaK"
                     /locus_tag="ACHGMT_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFN6836142.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            6872..8038
                     /gene="dnaJ"
                     /locus_tag="ACHGMT_002429"
     CDS             6872..8038
                     /gene="dnaJ"
                     /locus_tag="ACHGMT_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFN6836143.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         2822 a         1361 c         1772 g         2191 t
ORIGIN      
        1 agttcgggca acagcagaaa agtatataaa tttccattag ctttaataaa tcaagagcaa
       61 ttgattgaag ataaagatat ataaccagga agaaaatcaa actagacata ttcgtaattt
      121 atctaatgct aaattagagg aagaagcaag aaaagttagt aattataata aaaaaatggc
      181 tgcaaaatat gttaacgaag aggagttttg cttctcaaaa acaaaaatat ataaacgtaa
      241 tcccttcgta tctgaatatg ttaaacgact tgcaaaaggt atttgtcaac tttgccaaga
      301 aaaagggcca tttataaaag acggtgtacc ttatttacat tgtcatcata tagaatattt
      361 gtcgcaaggt ggaaaagatg tgattgaaaa ctgtatcgcg ttatgtccca attgtcatgc
      421 tagaattcat gaacttgagt tacctgacga taaagaaaaa ttgctgaaga tcgttgaaaa
      481 aagagaaaat agcttttcta aaataccgta atttattttt ataggacaaa taagctaatg
      541 tgagctatgc cttctatata agactaagtt ctattataag taacgaagta attctcgttc
      601 cttcctgtag taatattttt taaataacct ataggcttct tcaaaagtac atcctccagt
      661 aaattctaag tactggagga ttctttttct agtgttgctt tactcatgaa aaagtagttg
      721 gctacttcat caaaatttct gaactttttt aatgtataat aaagagctcg atcatttact
      781 acaaggatgc tagctcctac attttcacag ttttttagca agtaaatttt tctaaaatca
      841 catcaggaaa tgcatgagat tctttaagag aggcaattat agggctatga ttacggttcg
      901 tgtaagtgac gccaaaaaag aatggctttc ttatatggct gaattttatg ggattacttt
      961 atctgaactt ctaaaaaact attcgatgga acaactagaa gatgaataca atcgtcaaac
     1021 ggctgaaatt gcccataaac attggataag aagtgggaaa acaacagtat tcatggaaga
     1081 atccttaatg aatttggtgg gctggaatga cctgccgttt aaaattcacg cccgaaattc
     1141 agaagcagct ctgattggca aatacgtgta ttggtagaga tagaagataa tcaattcgtt
     1201 gttatcgcta ttcaagtagg gcaccgaaga aatgtctatg attaagagac tgtttatttt
     1261 tacaaaaaaa taataattgg aatctagtta aaactcttga agtgagagat ttgactagat
     1321 ttttttatga ataaaataag gacaaaattg tttaggaaga atcatgagtg taaaaaaatc
     1381 atccaagaag tcgattcttg gattttttag tggatttgtt cgccaaatac gtttagtatg
     1441 cgtatatgag aggttgtaag caaagcgttg tggtctgagc acttaaggag gaatctgtga
     1501 aaatagcttt tcatattttt gcagattttg acttaatgcc gaaggtcagc tttgcgatta
     1561 ccgtttatcg agaggctgta aaagcaccgt gtagctccga tgacaataag caagcatttt
     1621 tagagctagg tttccttgat tctaaatatg cggcttattt caatgagttg gtgcttgaac
     1681 gcgattttaa gaaggtcgaa aacaggcacg acttttcgac gtaaggagaa agaatttcaa
     1741 tgatagaaac aataaatccc gcagaaaaca aggtttctgc gtacattcat atcccatttt
     1801 gcgagcatat ctgctattac tgtgacttta ataaagtttt tttagaaggg cagccagtag
     1861 atgagtatgt ggaaatgctg ctgaatgaaa tgagattaat gctggaaagt catcctacag
     1921 cgaatttgga aacactttat gtaggaggcg gaacaccaac ttctttatct gctaaacaac
     1981 ttgatcgact attatcaggt gctagagaga tcctgccttt tcaaaatggt aatgagttta
     2041 ctgtagaagc gaatcctggt gatttgaccc gtgaaaaact tcaagtgatg aaaaactatg
     2101 gcgtcaatcg tttatccatg ggagtgcaga catttgacaa tcgcttattg aaaaaaattg
     2161 gacgtaaaca tactgcagaa gatgtgtatc aaacaatgca atttttagaa gcagaaaatt
     2221 ttaccaatgt aagcatcgat ttgatttatg cattaccggg acaaacacta gaaggatatc
     2281 gggatacttt ggaaaaagct ttggctttgg atctgcctca ctattctttg tactcattga
     2341 tcctcgaaaa caaaacaatg ttcatgaact gggtacgaca aggaagactc gaactgcctg
     2401 atcaggaaac ggaaaccaga atgtttgaag agacgattga agcaatggaa aagcatggac
     2461 gtcaccaata tgaaataagt aactttgggc tctccggaca tgaatctcaa cataacctca
     2521 tgtactggaa caatgaccac tattttgggt ttggagctgg tgctagcggt tatcttggag
     2581 atattcgtta ccgcaacaaa ggaccaatcc aacactattt acgcccatta cgagcaaatg
     2641 aacgcccggt attggaagaa gaaaaattga cacgaaaaaa tcaaatagaa gaagaaatgt
     2701 tcttaggact aagaaaaaaa acagggatta ctaaaaaaca tttctatgat cgctatcaaa
     2761 aaacaattga atcggtgtac ggtgatgtgc taagggaatt agagcaagaa gggttacttg
     2821 ttaatgaaga agaccgtgtt tatttgacac caagagggac ttttttgggg aacaatgtgt
     2881 ttgagcgctt tttatttgaa aaatagtttg aagaatattt gttttttatc gaaacttgaa
     2941 aagaaaaagt ttagaaagtt tgacaatgag gtattgaagt attgatataa ctggatttac
     3001 atcaattatt tagaattagc actcttgtgt ttagagtgct aaaaaatgga gtctttctct
     3061 tgacaggaga agggcttctt gctatattta taagtgaatt agcacttaga atatatgagt
     3121 gctaatgaga ggtgaggaag atgttaacaa agcgacaaaa cgacatttta cgtctgatca
     3181 tccaacacta tacgagtagt ggcgtgcccg ttggttccaa aacattaatg tctgagggag
     3241 tcaaggccag ctcagcaacg attcgcaatg atatgaaagc attagaagat gaaggattgc
     3301 tgttgaagac tcactcttct tctgggcgga ttccatctgc tttaggctat cgttattacg
     3361 tggatcattt gttaaaacct gctcgcgtag cagaagatga tttgctgctg attcgtcagt
     3421 ctctaggcag agaatttcat gaaatcaatg agattattaa gcaatctgct gagatattat
     3481 ctgagctgac aagctatacg actttttcat tgggacctga aataaaagac cgccgactga
     3541 ctggctttcg tatcgtaccc ttgaataatc gacagattat tgctatcgtc gtaacagata
     3601 tggggaatgt agaaagccaa gtgttcactc ttcccgaata tatgggaagt caggaactag
     3661 aaaaaatggt acagattgta aacgaccgtc tagtcggaga tcctctgatg acggtgtacc
     3721 ataaacttcg tacggagatt ccgatgattc ttcataaata tttccagacg acagaaggga
     3781 tgtctcagtt atttgacacc gttcttggtc atgtttttga agaaaaggtt tatgtcagtg
     3841 gacaaatgaa tctcttggat tatgagcccc gtcaagatct tgatcagttc aaatcgatgt
     3901 tttcttttat gaaagactca gacggactta cacaaatgat cactccgatg gatagcaata
     3961 ttcatatcaa gattggtgct gaattaggca atgatttatt gcagaatatg agtatgattc
     4021 aagcaagtta cgagatagat ggtcatggaa gaggtacaat agccttgtta gggcctacta
     4081 gtatgcctta ttctcggatg ttcggtctag tcgatgttta tcgaagagaa ctagcagcta
     4141 aacttgctga ttactaccgc tcgcttgact tgtcaaactc gtaatgtgaa taacgaagct
     4201 atttgcagct gatcgttctc tctttcttag taagagcaat caaacagctg actgaataat
     4261 gaaaggaaga ctgtatcgtg aaagaaaatc aagaagaact agataaagaa atgacagatg
     4321 ctcagccaga accggaaatc gatgtcgaag ctgcagaaga ttcgggtatt tcagaagctg
     4381 aagcggaaga attcgaaaca gcgaaattaa aagctgagtt agaagaaatg gaagataaat
     4441 acttgcgggc tcgtgctgaa atcgcaaata tggcaaatcg aggaaaaaat gagcgtgagc
     4501 aattacaaaa ataccgttca caagatttag ccaaaaaatt acttccatca attgacaacc
     4561 tggaacgtgc attagccaca gaagtgtcag atgatcaagg ggcaggtttg aaaaaaggcg
     4621 tggaaatggt cttggaaagt ttgcgtaacg cactggaaga agaaggaatt gaaaaaatcc
     4681 ctgctaaagg agaagccttt gatcctaatc ttcatcaagc agtacaaact gttccagcga
     4741 ctgaagatac accagccgat acgattgtag aagtactaca agaaggctac aaattacatg
     4801 atcgcgtgtt aagaccaact atggtcattg ttgcacaata attgtgcgga tataaaactt
     4861 acactttcaa ttaaagaagg agacgttaat tatgagtaaa attatcggta ttgacttagg
     4921 tacgactaac tctgcagtag cagtattgga aggaaacgaa gcaaaaatca tcgctaaccc
     4981 agaaggaaac cgtacaacac cttctgttgt atcattcaaa aatggagaaa tccaagtagg
     5041 ggaagttgca aaacgtcaag ctgtgacaaa cccaaatacg atttcatcaa tcaaacgcca
     5101 tatcggtgaa gcaggttaca aagtagatgt agaaggtaaa tcatatacac cacaagaaat
     5161 ctcagcaatg atccttcaat atatcaaagg attcgcagaa gactatttag gtgaaaaagt
     5221 cgaaaaagca gttatcactg tgccagctta ctttaatgat gcacaacgtc aagcaacaaa
     5281 agatgctggt aagatcgctg gtctagaagt agaacgtatc gtcaatgaac caactgcagc
     5341 tgctcttgcc tatggtttag ataaaacaga tcgcgacgaa aaaatcttag tattcgacct
     5401 tggtggcggt acttttgacg tatctatcct tgaactagga gacggtgtct ttgatgtatt
     5461 gtcaacagct ggcgacaacc atctaggtgg ggacgacttt gataacaaaa tcatcgacta
     5521 catggtagca gaattcaaaa aagaaaacag cattgacttg tctcaagaca aaatggcttt
     5581 acaacgcttg aaagacgctg ctgaaaaagc gaaaaaagat ttatctggcg tatcaagcac
     5641 acaaatcagc ttgccattta tcacagcagg agaatctgga ccattgcatt tggaaatgac
     5701 attgacacgt gcaaaatttg atgaattaac agctgactta gtagaacgta caaaagtacc
     5761 agtacgtcaa gcgttaaaag atgcaggtct gtcacaatca gaaatcgacg aagttatttt
     5821 agtcggtgga tctactcgta tccctgcagt tgtagaagca gtacgtaaag aaacaggaaa
     5881 agaaccaaac aaatcagtta accctgatga agtagttgct atgggtgctg ctatccaagg
     5941 tggcgtgatc actggtgatg tgaaagatgt tgttttactt gacgttacac cattgtcatt
     6001 aggtatcgaa acaatgggtg gagtgttcac taaattgatt gaccgcaata caacgattcc
     6061 aacaagtaaa tcacaagtat tctcaactgc ggcagataat caaccagcag tagacatcca
     6121 tgtattacaa ggtgaacgcc caatggctgc tgacaacaaa actctaggaa gattccaatt
     6181 gacagatatt ccagcagctc ctcgtggtat tccacaaatc gaagtaacat tcgatattga
     6241 taaaaacggg attgtaaatg tatctgcgaa agatctaggt acacaaaaag aacaaaaaat
     6301 cacgatcaaa tcttcttcag gtctaacaga cgaagaaatc gaacgtatgg tgaaagatgc
     6361 agaagcaaac gctgaagcag ataaagctcg taaagaggaa gttgacctac gtaatgatgt
     6421 agacgcattg ctattctctg tggacaaaac attgaaagaa ttagaaggca aagtggacga
     6481 agaagaagtg aaaaaagcag aaacagctcg tgacgaatta aaagccgctg ttgaagcaaa
     6541 caacatcgag gaaatgaaaa ctaaacgcga tgcattgaac gaaatcgttc aaaacttgac
     6601 tgttaaacta tacgaacaag ctgcacaaca acaagctcaa gaaaatccag aagcagctca
     6661 aggcggagca gatgatgtcg ttgatgctga ttttgaagaa gtcgatggcg acgataaata
     6721 atcagttcac tgaatttcga gagaaggcca aagttgctac tttggctttt tcttttgaaa
     6781 aatagtttga agtatcggtt tttgtttcaa acaaaatatg atatgataac aagcgacgtg
     6841 aaaatgatga gaaccaatag gaggaaagcc tatggcaaca aaacgtgatt attatgaagt
     6901 cttagggctc tcaaaaggag catcagaaga tgaaataaag aaagcttatc gtaagctttc
     6961 gaaaaaatac catcctgaca tcaataaaga acctgatgcg gaagaaaaat tcaaagaagt
     7021 atcagaagca tatgagattt taagtgaccc gcaaaaacgc gctgcatacg accaatacgg
     7081 gcatgcaggc acagatccaa attacggtgc tggtggagct ggtggcggat ttggtggatt
     7141 cggcggcttc tctggtggcg gcttcggtgg ttttgaagat atcttcgact ctttctttgg
     7201 aggaggcggt cggtcagtcg atccaaatgc gcctcgtcaa ggtgctgatt tgcaatatac
     7261 ggtcgacttg tcttttgaag aagcgatttt tggtgtagaa aaagaaatca agtataatcg
     7321 ggaagaaatc tgtcatactt gtggtggaaa cggtgcaaaa ccaggcacac aaccaactac
     7381 ttgtcataaa tgtcatggtg caggtacgat caatgtagaa cgccaaacgc cacttggacg
     7441 agtgatgagc cgtcaaactt gtgatgtttg tcatggcaca gggaaagaaa tcaaagaacc
     7501 ttgtccaacc tgtcatggct caggacatga gaagaaagca cattcagtta aagtgaatgt
     7561 accagctggt gtagaagaag gacaacaaat gcgtttagct ggtcaaggag aagctggaga
     7621 aaacggcgga ccatttggtg atttgtatgt ggtttttcgt gtagaagaaa gtgatatctt
     7681 tgatcgtgaa ggttctgaaa tttactatga attgccttta agttttgtac aagcagcttt
     7741 gggggatgaa gtccaagtac caactgtcca tggagatgtg aaattgaaga tccctgctgg
     7801 aacacagaca ggtacgaact tccgtttacg tggaaaaggc gctccgaagt tacgtggtgg
     7861 tgcaacaggc gaccaacatg tcaaagtaaa actgatcaca ccgaaaaatc taaatgaaca
     7921 acaaagagaa gcattacgtg cattcgctga agctggcggc atcaaagttg aagaacagca
     7981 agaagacggt ttcttcgata agtttaaaga tgcttttggc gggaaaaagc ggaagtagtt
     8041 tttctagtaa aaatataaag tagacttgcc caaattttct atttctagta aaaacgctaa
     8101 gtcattaata attaaaaacc agacgaacaa tcatacgttc gcctgg
//
LOCUS       DAZBSA010000111         8090 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000111 DAZBSA010000000
VERSION     DAZBSA010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.112"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            382..570
                     /locus_tag="ACHGMT_002430"
     CDS             382..570
                     /locus_tag="ACHGMT_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFN6836144.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            648..1661
                     /locus_tag="ACHGMT_002431"
     CDS             648..1661
                     /locus_tag="ACHGMT_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN6836145.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1728..2762)
                     /locus_tag="ACHGMT_002432"
     CDS             complement(1728..2762)
                     /locus_tag="ACHGMT_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN6836146.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            2877..5102
                     /locus_tag="ACHGMT_002433"
     CDS             2877..5102
                     /locus_tag="ACHGMT_002433"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFN6836147.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            5120..7003
                     /locus_tag="ACHGMT_002434"
     CDS             5120..7003
                     /locus_tag="ACHGMT_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFN6836148.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            6984..7958
                     /locus_tag="ACHGMT_002435"
     CDS             6984..7958
                     /locus_tag="ACHGMT_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFN6836149.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         2613 a         1475 c         1707 g         2295 t
ORIGIN      
        1 gttagtctac attattttgg cgatttgcca ataaggactg ttgagttgtg caaaatcaaa
       61 tctgggtcag aatgaacgtc attctgtctc agatttttcg ttatttcaat ttgattaact
      121 gattgatcaa aaagattttt attttcatat tctcaaatga ttcaacacta aatatacatg
      181 tttgacgcgg aaattattat tcgattttgg cggcatcagg ggggatgacg acgtagcaga
      241 cccattattt tactcaagaa aatgtccgtg cctattattt agaatacgat agtgaacgtg
      301 ctgggagttt tgttctttta gagaaagtct ctgaagataa attagttgtc ttagattttg
      361 actgtaacga aaaatggcga aatggaagag caccagcaag tgattgaccg gatctacgaa
      421 gcgacacgat atctgccttt ggatcgtttg tgcttaagtc ctcaatgtgg atttgcttca
      481 acagaagaag aaaatgtttt gtttgaagaa caatggaaaa aatttctttt gtcaaaagca
      541 ttgcagaaga agtttgggga aaatcattaa atcatctagg agagcttgat aggaaataac
      601 actatggtat aatcagaatg cttaatacta actactgagg gagccttatg aaaaagaaaa
      661 ttcttaaatt tatcggatgg ttcttcggaa tcattcttgg gatcgcgttt gcaatctttc
      721 ttgccttcca gctttccccc agacctggag cattcttgat caaccatatg ttttcaaata
      781 ctgttcaaat cactgataaa aaaacgttcg atcaagcaga aaaaaacgtg gcgaaaaaaa
      841 cagatttgac gtatgagtcc aagtataaag agaatactta tgacatctac tatccaaaaa
      901 acagcaaggg gcctgttcct gttctatttt gggtacatgg tggaggattc gtcggcggag
      961 ataaatcggg tgtcaaagaa tttgcgacaa aattagtttc agacgccaac atcgcagtgg
     1021 ttgcgatgaa ttatgaatta gcgcctgatt cagaataccc taatcaagtt cttcaagtga
     1081 atgaactgat caaagattta ttagctgaag aaaaagacga caaattgcta gacatggaac
     1141 aattattttt tggtggagat agtgcaggtt cacaaatcgc attgcaatat gcagctatac
     1201 aaacgaatga tgcgtatgct aaacaaatga aaatcaaaca gctacttcca aaagatagtc
     1261 tgaaaggaac catttcttat tgcggacctg ttgatttgaa gctaacagca cagcagcatt
     1321 cggatgatcg atttatgaag tttttcgtca aaaccgttgc gtggtcgttg attggaacaa
     1381 aagattggaa aacgagtgag caattgaaag aagctagtgt ggctgaccat gtttctaagg
     1441 aatttccgcc aacgtatata actgacggaa atgcatactc attccaggag caaggtcaag
     1501 cattagaaaa tcggttgaca gaattaaagg tacccgtgca atccttgttt tatacagata
     1561 caaaaaaaga gattacccat gaataccaat ttgattacac gctaaaagaa tcgcaaaatt
     1621 gttaccagca gacattggat tttgtgaata agtataagta agaggttgtg aaaaagccgt
     1681 ttcgttttga gtatgaagaa acgaagcggc ttttcacaac ctctctttca ataccttttt
     1741 gttcttggga taaaactttc cctaacaatc aatcgtgcag gataagtcaa aatgattggt
     1801 tcgtcccgta taccgtcaat tcgctccaat gccattcgaa cagctgcttg cccaattgat
     1861 ttcgcattga tttgaacact ggtcaagcca ggggagaggt ttgcgattgt ctcgatatta
     1921 tcaaagcttg ctacaacaat ctcttctcca atttggatat ttttttgttt caatgcccga
     1981 tagacaccaa ttgccattgg atcgctcccg acaagaatgg cagtaggaag aggggattca
     2041 ttttctaata gattagtcgc aagtacctca ccttgttttt ctgtccagcc acctagatga
     2101 taagacatat atttttctaa gtgatgatcg atcataaaat gctgataggc acgcatcctc
     2161 ttttcattta tagaaatctg cttcccaccg aattcatcca tatcgacttt atatcccccg
     2221 atataggcta ttcttttatg accattttct aagaacaatt ccagtacggt tttagtcatc
     2281 cgttcaagat ctgcatacac catatcgacc tcttgctgga tgtcagggtt atccacgacg
     2341 actatttgca tgttttgttt taacaaggcg gtaatcgatt ctttcgtaac agtcccaaca
     2401 acgatcaccg cacctaactg atctagatct tcccattgtt taggattatc agatagatta
     2461 tagattcgat tcatccctaa atgcagacgt cgagcttctt cttccatccc tactcgcaaa
     2521 tgtttgtaat agatatcctg ttcttcgctc gctttgccga tcgtagagat cacaccaatc
     2581 gaaggcattc tctttttgat atatttacga gtactgatgt cataatccaa cgcagcagct
     2641 gcctctacta tccgttgccg agtagtctga gccacatgga tcgtctgatc gttattcaac
     2701 acacgggaaa ctgtagcagg agagacattt gcctttcgtg cgacatctct aattccaacc
     2761 atttttacac actcctcaaa aaaataaaaa atttttattg acaaaattgt ttagtaaaca
     2821 gtatacagga attataaaaa aaagtaaacg gtttccgttt tgttgggagt gaaatgatgg
     2881 atattacgca tagaaaaaca gataaaaatt tcatcttatt tgatgaaaag agccgagtct
     2941 tccatttacg aaatacattt ctttcttatc tgataaagat agaagaaagc aatgtattgg
     3001 cgcatattta ttttggcaaa ccagtcaagc agtacaaaga caataaaaat tatccccgaa
     3061 gagatcgtgg attttcagga aatgttccct taaatcctga ccggtctctg tcgaaagata
     3121 cactaccgca agaatactct tctcatggca gcatggactt tcgaacgccc gcatcgatca
     3181 ttcagcgcaa gaatggatca gacttactgg atctgagata tgacagccat tatattacag
     3241 atgggaagcc agagatcgaa ggcttgcctc aaacttatgt gatggacaag tcagaggcac
     3301 aaacgttggt gatttcactt aaggatagag aaacagcgat ttatttcgat ttgttctata
     3361 ccatatttac tgatagagca gtgatcacac gatctgtaaa gataagaaat gaaaccggag
     3421 aaacaatcaa actagaaaaa gcggctagtt tccagctaga ctttgcccat actaggcgat
     3481 ttgatgaagt cattgctttg ccaggagcac atgtcaatga aagacagatt tcacggcaat
     3541 ctgttttatc tggaacaaaa gtatttgaaa gccgtcgagg aacgagcagt caccatatga
     3601 ataactttat tgctttagtt catcatcata cgacagaaaa tactggagaa gcgatcggac
     3661 ttcaatttgt ttattccggc aaccattctt tcgaacttga aaaagatcag atcaatcaat
     3721 tgagagtcgt cggtgggatc aacagccatc gtttttcgtg ggaattgaat gcaggccagt
     3781 cttttcaaac acctgaaatg atcctttctt actcaagtca gggattgaac aaaatgagtc
     3841 agatccacca tgagttgcta cgtgaaagaa tcgcaagagg tcgtcatcaa tttgctgaac
     3901 ggccgattct ggtcaataac tgggaagcca cttactttga cttcaatagt gaaaaaatca
     3961 aggcgatcat tgatgaagca aaggagctag gcatcgaaat gttcgtatta gatgatggct
     4021 ggtttggaaa aagagatgcg gacaattctt cattaggaga ctggttcgag tatgaaggca
     4081 agttgacaaa tggattacgt gaaatagcag attatgctca tagcaaagga ttgaaatttg
     4141 gtttatggtt tgaacctgaa atgatctcgg ttgattctga actgtatcga acacatcctg
     4201 attttcttat gcaggagcca ggtagaatgc cttctgcttc cagatcacaa catgtactgg
     4261 attttacaag gttagatgtt cggcagacga ttgagaaaca aatgagaaag attttagata
     4321 cgattcctct tgactacatc aaatgggata tgaaccgttc gctttcagat gtctattcga
     4381 ttacccttga cccacagaga caaggagaag tagcgcatcg ttatatgctc gggctttatg
     4441 aactacttga acatttgaca actgattatc cggaaatctt atgggaaggc tgttctggtg
     4501 gaggtggacg gtttgatgcc gggtttattt attatatgcc gcagtcatgg acaagtgata
     4561 acacggatgc agtggagcgt atgaaaatcc aatatggaac aagccttgct tatccgattt
     4621 cgtccatcac agctcatgta tcagctgtac caaatcatca gaccgacaga tctacttcgt
     4681 tgaaaacaag aggagaaaca gctatgagcg ctgtttttgg ttatgaactg gatttgacaa
     4741 agctgtctcc tgaagaaaaa aagcaagtaa aagaccaaat tatatcctat caaacgattc
     4801 gtccggtgat ccagtatggc cattattatc gtttggcgag tccttttgaa gaaaatattg
     4861 ccgcatggat gtttgtgtct cctaaacaag atgaagctat tgtgttttta gggaggatac
     4921 tagcttcagc tcaaccagcg ttccatgaag tatatctgat ggggttagat gatgaggcac
     4981 tttatcagga acagacctcg aagcggatat tttcgggggc cgaattgatg acagttggac
     5041 tttacttccc cgattttcaa ggtgatttcc aaacagaact gcttcatttc aaaaagttat
     5101 gagagagaag gaaaaaagta tgaaagcaaa tataaaatgg ttagatgatc cacaagtttt
     5161 tagagtgaac caatcaaatg cacatagcga tcatgtttgt tatgcaacaa gggaagaagt
     5221 agaaaccaca tcctccctta aacagtcttt aaatggatca tggcgttttt gttattcgcc
     5281 aaatccttcg gtacgaccaa aaacatttta taaaaaagat tttgatgcct gttcttttga
     5341 tgaaatcact gttcctagtc atattgaatt agcaaattat gacaagatcc attacatcaa
     5401 tacgatgtat ccgtgggagg gacatgtatt tcgccgtccc cctttcacga caagccctga
     5461 gaaagaggcg gaagggatct tttcagctgc taaagacaat ccggttggtt cgtatttgac
     5521 gacttttcga ttagaagaag gtctaaaggg aaaacaaatc tcgattcatt ttgctggtgt
     5581 agaacaagca atgtttattt ggttgaatgg tcattttatc gggtatgccg aagattcgtt
     5641 cactccgtct gaatttgatt taacacctta tttggaagaa gaaaatattc tagctgtgga
     5701 ggtttacaaa agaagtacag ctgcattctt ggaagatcag gactttttcc gattttttgg
     5761 gatcttcaga gatgtcttct tagttgctcg aggacaaaac catttagaag atctttggat
     5821 caaacctcgc ttagatgaca aatgtcaaaa tggcaaactt gaagtagatt tgtcttttga
     5881 gcaagtagcc aaaaacagtc agctgaatgt agcgatacta gatcctgatg ggaaggaaat
     5941 ctatcaacag caatatccgt taaaagagca aatgacgatt cgcatcgatg gatttgaaaa
     6001 tgtggcactg tgggggcatt ttcagcccaa tttatataag cttgaattgg agacgataga
     6061 cgaagaaaaa agagtaacgg agtatattga ctattccttc ggttttcgta aaatcgaaat
     6121 caaagataaa atcatctact tcaatggaaa acggatgatt atcaacgggg taaatcgaca
     6181 tgagtggcat ccgaaaaaag gacgcgccat cactatagaa gatatggaaa aagatttggc
     6241 tatcataaaa aatgcaaaga tcaacggcgt acggacatct cattacccca accagatcac
     6301 ttggtattat ttatgcgacc aagcaggaat ccatgtgatg gcggaaacga acctcgaatc
     6361 ccatggctca tggcaaaaaa tgggcgcatt tgagccttct tacaacgtac caggtgcagt
     6421 acctcagtgg caggcggctg ttcttgatcg agcaaaaacg aatttcgaaa cattaaaaaa
     6481 ccatccaagt attctttttt ggtctcttgg aaatgaatct tatgcggaag aaaacattcg
     6541 acaaatgaac gattacttca aaaaagtaga ccccgatcga cttgtccatt atgaagggaa
     6601 ttttccaaat ccagcattcg aagattcgat ttcagatgta aaaagctgca tgtatgcgcc
     6661 accagaagag atcattgact accttgaaaa taaagcggaa aaaccgttca tcttatgtga
     6721 atatatgcat gacatgggga attcgttagg tggaatggat tcttacatga cgttgcttga
     6781 tcgttatgaa atgtaccaag gaggctatat atgggatttt aaagatcagg cgatccaagt
     6841 gactgatcca atcacaggca gagaagtgat gcgctatggc ggtgactttg acgataaacc
     6901 aagtgactat gaattctcag ggaatgggat cgtttttgca gatggacaag aaaaacccgc
     6961 catgcaggag gtaagatatt attatgaaaa atacagtaaa taaaagtcat atggatacgg
     7021 aaaaagttgc aatcgtcttc ggcgactgta cattaggtgt caaatcgggg aatacgcatt
     7081 atattttttc ttatacaaga ggcggactgg aatcgctcaa taaaaacgga aaagaatggc
     7141 tataccggga aacacttcca gttttttggc gagcaacgac cgataatgac agaggtagcg
     7201 gatttggatt taaatccggt atgtggttag gagccggatt gtatccaaaa gtcaaagaaa
     7261 tagaagtact ggtggacaat aaaaagatcg atttgccgat tgctccagta aataatcaat
     7321 attcaagtaa tgaatacgct gaaacggttg aaatcatttt tacactggaa atcaacacag
     7381 taccaaaaac ccaagtgaca atttcctacc aaatcgctgg agagggtaaa atgcttgtca
     7441 atatgcatta ttatggacaa aaaggattac cggaattacc cgcattgggt ttaaggttca
     7501 ttatgccaac gaaagcaaaa ttttttgagt atgaaggcct atcaggggaa acatatccgg
     7561 atcggatggc tggtggtgtc gaaggtattt ataaagtggc aggactcccc gtttccccat
     7621 accttgttcc ccaagaatgc ggcatgcata tggaaacgaa atgggtagaa ctaacaagaa
     7681 atcaaacgtt ggataatacg gataaagatc aagaagattt ttcgttgcga tttgaacagc
     7741 aggatacgcc atttgctttt tccgctttac cattcaaagc agaagaactg gaacaggcaa
     7801 cacatataga agaactaccg ccagaaagac gaacggttct tacgattttt gcaaaagtaa
     7861 gaggagtcgg gggtatcaat agctggggag cggatgttga gtcaccttac cgaatcagca
     7921 gcgaagagga ccatgaaatc ggctttatta ttcagtagca atctttagat tattatttga
     7981 aaaacggagt ttacaagaca aagctgcttt attctatatg tcatgaacaa gagagtaaaa
     8041 aagttctcag atacttttgt tcatgactat tttcttcaat aaaaccaatt
//
LOCUS       DAZBSA010000112         8072 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000112 DAZBSA010000000
VERSION     DAZBSA010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.113"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(121..969)
                     /locus_tag="ACHGMT_002436"
     CDS             complement(121..969)
                     /locus_tag="ACHGMT_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN6836150.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1193..3133)
                     /gene="thrS"
                     /locus_tag="ACHGMT_002437"
     CDS             complement(1193..3133)
                     /gene="thrS"
                     /locus_tag="ACHGMT_002437"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFN6836151.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3470..3649)
                     /locus_tag="ACHGMT_002438"
     CDS             complement(3470..3649)
                     /locus_tag="ACHGMT_002438"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFN6836152.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3696..5519)
                     /locus_tag="ACHGMT_002439"
     CDS             complement(3696..5519)
                     /locus_tag="ACHGMT_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFN6836153.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5797..>8072
                     /gene="helD"
                     /locus_tag="ACHGMT_002440"
     CDS             5797..>8072
                     /gene="helD"
                     /locus_tag="ACHGMT_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFN6836154.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
BASE COUNT         2365 a         1710 c         1458 g         2539 t
ORIGIN      
        1 aataaactta tttttgaaaa ttatgttttc ttaagtcgtg aaacactttg atggatcatt
       61 tatttgtgga aaactacgtt ttgttattct aaaaaactta tataaaaaat agtaaggttt
      121 ttatttcgaa tccataaaat attcttggat tgcttctcgt gtttttttca ctttttcgct
      181 aagcttttcg tttttagcca tatagaaata aaaaatgata tcgatcgtag caaattgggc
      241 caaaagtgaa ttagttgccg cacttcgatt cgtgatttct tttggtctag atgtttgtac
      301 gagtacatcg gctatcttgc taagcgggtt atttccaaat tgcgtcaacg caatgatttc
      361 gatattcatg cttttcgcca gctcggctaa tgcgactaca tctgcacttc tacctgagtt
      421 tgaaacaagc caaagcactt tctttttctc attgctgaca agttgcggga gcaagacgtg
      481 gatatctttt tcaaaaatga ttggcttacc gacacgagac catttttgca gaatatcttc
      541 tactactaaa gaagaggcgc caaccccata taaaaaaaca gtctctgccg attccaaaca
      601 ctctacgact ttttggatcg tttcttcttc tagaatatct actgtttctt ttatcgctac
      661 ttgtgcattg ttaccaagtt tgtttttgat agagtaaaag gactcatttg cttctatatc
      721 actaaaacct tcatcgattc cagtctgtag ttctgttgaa atcgtgattt tgaagtccgt
      781 cagactttga aaaccgattt ttttggaaaa acgtacaact gtcggcgcac tcaacccgga
      841 agcttctgct aattcattag ctgtcattgt agggaccttc tcaatattac cgataatgta
      901 gttggccaac tttttttctg ctgaagaata atcttccatc atactttcaa tacgactaat
      961 tacgctcata agttcctcca cccttttaat gatagtctat gtaagtatac catatcagac
     1021 ccttattcac ggtcgtatac tggataaaaa acaaaaaaac aatccgccca acagaatatc
     1081 ttatcccatc aatcccacag attctattga gcatctgtgt ctttgaggaa tacctgttat
     1141 ctattgttcg gattgctttc tatctactac ttttttacag gaaatttacc tattagtttt
     1201 ctctgctgta atttttgact tctgcttcca tagcatcaat aaagatattc aaatcttcta
     1261 cagctgtttc tttacttcca taacgacgta cgttcactgt tgcatcttcc aattccttgt
     1321 ctccaacaac taattggtaa gggatctttt gtgtttgaga ggcacgtatc ttatagccca
     1381 ttttttcatt acgatcatca acttctacac gcaacccctt catttgcaaa cgttctttga
     1441 tttcataagc ataatcgcca tgtgcatcaa cagaaacagg gatgattgtt gcttgtatag
     1501 gtgccaacca tgttggaaaa gcgcctttgt atacttctgt taaataagcg acaaaacgtt
     1561 ccattgtaga aacaattccg cggtggataa ctactggacg atgcgtattt tccccgtctt
     1621 ctccaacata tgtcaaatca aagcgctctg gcaataagaa gtctaattgt atagttgata
     1681 atgtttcttc tgtaccaaga gctgttttga cttgaacatc tagttttggt ccgtaaaagg
     1741 ctgcctcgcc ttctgcttca aagtaatcta gttctaattc atccatagcg gctttcagca
     1801 ttgcttgggc tttttcccac attgcgtcat catcaaaata tttatctgta ttgtttggat
     1861 cgcgatagct caagcggaaa cgataatctg taatattgaa gtcagcatat actgccacca
     1921 tcaattcaag cgtacgttta aattcatctt tgatttgatc aggacgaaca aatgtatgac
     1981 catcattcaa cgtcatttct cgtacacgtt gtaaacctga caaagcacct gatttttcat
     2041 agcgatgcat cattcctaat tcagcgatac gaattggtaa ttcacgataa gaatggatcg
     2101 tgtttttgta gaccatcatg tgatgagggc agttcattgg gcgaagaaca agcatttcgc
     2161 catctcccat atccattggc gggaacatgt cttcttgata atgatcccag tgtccagatg
     2221 ttttgtacaa ctcaacatct cccattattg gtgtatatac atgttggtaa cctagactaa
     2281 cttctttgtc tacgatataa cgttcgatcg tgcgtcggat tgttgcccct tttggaagcc
     2341 agaatggcaa accagaacct acttctgggg aaaccatgaa caagtcaagt tcttttccta
     2401 gtttacgatg gtctctttct tttgcttctt cacggagacg gatatattct ttcaaagctt
     2461 tcttgtcaaa aaaggctgtc ccgtatacac gttgcatcat ttgattgttt gagtttccac
     2521 gccaataagc acctgccact gataataatt taaatacttg tatacggcca gtggatggaa
     2581 catgtggtcc acgacacaaa tcgatgaatt cgccttgttg ataagcagta atcacttctt
     2641 cttctggcaa ttcactaatc agttctactt tgtacggatc acttgcaaaa atttctaatg
     2701 cttcctgttt tgaaagaaca cggcgttcga tcggcagatt ttctttgacg attttcatca
     2761 tttctgcttc gattgccggc aaatcttctg cagttacttg gttttgtcca ttatctgtat
     2821 cgtagtaaaa ccctgaatcg atagctggtc caacgccaaa gtggatattt ggatagaggc
     2881 gacgcatggc ttgggccatc aaatgagctg ctgaatgacg gactaagcca agtgcatctt
     2941 catggtctgg tgtgatgatc tcaatacttg catcttcctc gattggacga gtcaaatcaa
     3001 cgagttttcc gttcacttta ccagctaaag ctttttttgc taagcttttt gaaatgcttt
     3061 cagcaattgc caatgttgtt gttccagact caaattcttt gactgcacca tctggaaaag
     3121 taatttttat cataatagtt tcctccttga aatgttttga ttgatcagat catcaagctg
     3181 aaaaacaaaa aaatcctagc atctcttgaa aagagatact aggacgaaaa gttcgtggtt
     3241 ccacctaatg ttcaaacgca aatgcgttcc tcaagcgttg taacggtccg accgcccttg
     3301 cttcaacaag ggtttcgaaa gtggtcatcc tttggttttc tcctggaaaa gtttcagcct
     3361 agcttttcct ctcttatcga agactctcaa attcagttgg ctttctcatc aatcaattat
     3421 gtgtttattt aaccactaat aaatagaaaa atcaagtggg aaactaaact tattttttcc
     3481 attccccatt tacaccgtat gttccttttt tcatctcatt gatgatgaca tggatgtgtt
     3541 cttttggtgc ctgcgtgttt ttatgaacag cttccgtgat atctttcatc atattttcta
     3601 attgttccgg actgcgacct tctactaatt cgacatgtac gaatggcata atgctctctc
     3661 ctctttctga tcgttctttt ttcaactcga ttctatcata ctatgtttgt cttgggtact
     3721 gaatcaggaa aaatggctgg atcttttttt gctctcaagg cactcaaaat agagaccgta
     3781 tccacgactt cttgcaaaac agctccgaat aatgcgggga ttagtcccgt acttgcaatt
     3841 aacatcaaaa aaatacaaat aaagatgcca atcagtacag actgtttcgc tattctcatc
     3901 gtatctcttg aaatcactac tgcttttgct acacgcaaga gatcatcttt caagatgacc
     3961 gcatctgcac tttcactagc agctgtagaa ccatgagccc ccattgcaat accgatatct
     4021 gcagcagcaa gtgctggtgc gtcattcacg ccgtctccta ccatcgcaac aggtctttga
     4081 tctttaggga tactttctaa aatggtcagt ttatcttgag gcaagctacg ggcatgtaca
     4141 gaatctaatt ggactttttc tgcaatcgtt tgagcaatgg cagatccatc cccggtgatc
     4201 atcatcaaat ttttcacatc aagttttctt agctccttga ttgtctttgc tgcttctggg
     4261 cgaatctgat ccgaaaatag gatcgttcct gcaaaagatc gatcaattgt tacatagatt
     4321 gctgtattgg acgtttctat ttcttcagct gaaacaaaag aggcacgtcc aatctttact
     4381 tcatgattat tgactactgc ttggattcct tgaccggtca cttctttcag ttgactaaca
     4441 ggatacagtt ggctgttttt cacgttgttc actaacgaac gagctaagat atggctagat
     4501 tcctgttctg cactagctgc atacagaagt agctcttctt tttttattcc attgactggc
     4561 tgaatcgcct ctacttccaa tatccctttg gtaagcgttc ctgttttatc aaaagcaatc
     4621 gtttttgtac gtgcgagctt ttcaatcgtt gttccagttt ttacaacgat cccgttttta
     4681 cttgaacggc tcattcctgc aactaaagca atgggcgcag ctagaatcag tggacaaggt
     4741 gatgccacta ccagtacctc agcaaatcgt aaagggtctt ttgagaacca ccaagaaata
     4801 ccaccgatca aataagcaat caacgtgaaa gggactgcat aacgatcagc taaacggaca
     4861 aatcgggcag gcttttcttt tgattcttcg actagtttaa taatcatttg atactggctg
     4921 tctgcggcac gtttttcagc gatcatttgg atcgatccgt ctccatttaa agaccctgac
     4981 ataatctgat ctccaggttt cttggtttcc gggcgggact cacctgtcaa agaagattca
     5041 tcgaccgaag attctccttt ggtcactcga ccatctgcgg gtacaagttc gccaggcttg
     5101 actagtaact ggtcatcaat atttatttca tcgattacta tatcctgcaa ttttccttct
     5161 ttgaatcgat gggcaattct tggggtgtta tctaacaaag actgcagttc tttacccgct
     5221 cgtttattcg cgtaatcctc tagactatct ccgcccgtaa gcatgatcaa tatcatcaaa
     5281 ctagcccagt attctttgac ggctaatgtt gcaaggattg cggtaatcgc taatacatct
     5341 acgccatatc ttcctgaacg taacgtcttg atcatttcca aaaacatcaa gaaggcgaga
     5401 atccctccag taaatgcgac aatcgcaaat gcaagggccg gctggtgaaa aacaaattca
     5461 cttaataaag ctatcccacc aataagcaaa gtaagtaaaa attttttgaa atgtgtcatt
     5521 gttttttctc ctcttttatg tgacttagag aggttagtaa taaatttcca gctgcctctt
     5581 tcatcttaag catagtcctt gactagcact ttttaaaaca ataggcctta attgaaaaca
     5641 actatcacta tcagcgtttc caaaattcat cagttctaca aacaaaaata ttcagcaagg
     5701 attacttttt cgattcatca gttaaaaagt accttttttc gaaaaactat gctatacttt
     5761 taattcgtta tttacggcgg aaggagcaaa catgcgatga atgagaaaca actagaacaa
     5821 acgcacgtgg atgaaacgat cgaactaatc cagatcgaac aaaatatgct ggatacaaaa
     5881 caacaagaat tgactcagga aatgaatcat tcgacaaaag acacagctaa tcacaagatt
     5941 cgagctggtt ccaatgaagc tttttatgaa tctgctgttg aatatcgcca acatgagcag
     6001 gaattgcttt tgaaatacca tactcttgaa gcacagcaaa aacgcctgca aacgttgtct
     6061 gtcatgaaag atagtcctta ttttgcccgt atcgacttta aagaagaccg tgaaacagaa
     6121 acattatatc taggtatcgc ttctttaaga gatctggcag aagaaacaat cgttattgac
     6181 tggcgcgcac caattgccaa tctttattat gaaggtgaga ttggtcccgc attttatgaa
     6241 actgaaacag atagaataga tgtcgatctg cagttgaaac gacaatttaa aatccaagaa
     6301 ggacgtattt tatctatggt agatacgtcg gaagtaatca atgatgactt tttgctagag
     6361 atattggatg aatcatcaag caaccatatg aaaaacatcg tctctacgat acaaaaagca
     6421 caaaatgcaa tcattcggga cacaacaagt aaagtaatgc tgattgaagg aatcgccgga
     6481 agtggaaaga catccgcttt gcttcaacgt attgcttttc tgttgtatca taatcgcaag
     6541 tggctcgagg ctgaaaacgt tcttctattt tcacccaacc atttgttttc tgattacatc
     6601 tcgactgtat tgccttcatt aggtgaaagc ggcgtgccta cacagacatt caaaaattat
     6661 ttggtgcagc tgctgccaga gttttcttta ctagatgaac agcaacaaga aagtggtttt
     6721 ttgctagggg aaaaagatcc gatacaagtg atgaaaagcg gattgacctt agtcgatcag
     6781 attgatcgct atatccaagg catcacaaac tacggtccct tgttccgtga tatgaaaata
     6841 aatggccgta cgatcctatc aaaggaatcg attcgccaat ggtataaaga aacaaatgaa
     6901 caattacctt tacatcatag actttctcta ttacaaacga agctcctgaa aaaattaggc
     6961 ggtctgcaaa aagacgaaac acgtcagcaa tgggtcaaag atctggcgga agaacagctc
     7021 caagaactat acgcaacaga tccaaatctt gaatatacag aacaaaagga gcgttcatta
     7081 cgtaaaaaac tggccaacca aatcgtacgt aaaagattcc gtcgaatcca tcgagggatc
     7141 atgaattatc aatttgtcaa tataacgaaa caatatctcc actttctaca aactgtgcca
     7201 ctttctcttc taacacgaca taaaatcagc gagaccaggt ggcaggaaca tttgacaact
     7261 gttcgtacac acttgcgtca aagagaattg cgtcaagagg atgctgtact attcttttta
     7321 ctgatgcgcc gaacatatcc agtgacggtt acacacaaag ctcgctatat ctttattgat
     7381 gaaatgcagg atttcgcacc atcacaagtg gcattgctgc aagccttgtt cccaaaagca
     7441 aatttgactt tctgtggaga tctaaatcaa aatgtctttg gaaatgaaac gatcgttgga
     7501 tcattggaag accttttcgt ggaccgtgaa gtcactcgtt tccaattaac gacaagctat
     7561 cgctccacaa aagaaatcac tgattttgct aatcatttta ttgtgaaaga acatcaagta
     7621 gaaacaacag caagaaaagg agaccttcct ttgctcatcc aaggaacaac agatgatatc
     7681 aaattgaatt ggttagcaga taccattcga acgactcagg gaaaagcccg gtattggcgt
     7741 acagcaatca tagctaaaac acaagaagaa tgcgaagctc tttatgctca actgccagac
     7801 gagttgaaac aacaagtcca gctgatttta aatgagagtg actttatgaa gcgttccatt
     7861 attttacttc cagccttttt agcaaaaggt ctggagtttg accgagtttt cttgtgggat
     7921 gtagatgcag aacatttttc ttctcagcaa gacaaattga tcctttacac catgtgtaca
     7981 cgtgcgatgc atgaattgac cctttttacc tccaataatg aaagtccttt gttaaacaat
     8041 atggatattt catcttatga gaaaaaagta ct
//
LOCUS       DAZBSA010000113         7811 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000113 DAZBSA010000000
VERSION     DAZBSA010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.114"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(31..1383)
                     /gene="celB"
                     /locus_tag="ACHGMT_002441"
     CDS             complement(31..1383)
                     /gene="celB"
                     /locus_tag="ACHGMT_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFN6836155.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(1383..1901)
                     /locus_tag="ACHGMT_002442"
     CDS             complement(1383..1901)
                     /locus_tag="ACHGMT_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836156.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(1917..2243)
                     /locus_tag="ACHGMT_002443"
     CDS             complement(1917..2243)
                     /locus_tag="ACHGMT_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFN6836157.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(2256..4235)
                     /locus_tag="ACHGMT_002444"
     CDS             complement(2256..4235)
                     /locus_tag="ACHGMT_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN6836158.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(4256..4570)
                     /locus_tag="ACHGMT_002445"
     CDS             complement(4256..4570)
                     /locus_tag="ACHGMT_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFN6836159.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4738..5031
                     /locus_tag="ACHGMT_002446"
     CDS             4738..5031
                     /locus_tag="ACHGMT_002446"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFN6836160.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(5109..6221)
                     /locus_tag="ACHGMT_002447"
     CDS             complement(5109..6221)
                     /locus_tag="ACHGMT_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFN6836161.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            6374..7222
                     /locus_tag="ACHGMT_002448"
                     /pseudo
     CDS             6374..7222
                     /locus_tag="ACHGMT_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7575..>7811)
                     /locus_tag="ACHGMT_002449"
     CDS             complement(7575..>7811)
                     /locus_tag="ACHGMT_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFN6836162.1"
                     /translation="DATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKH
                     LPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         2495 a         1554 c         1262 g         2500 t
ORIGIN      
        1 tttttgtcac agttcctttt ttcttagaaa tcattttaac ttttcactag ctaattctgc
       61 tgccttttct ctttcttcat ttaataattt attatcatag aatttgataa atgggaacca
      121 gataatgaat gcgatgattg cacaaatgac tgctaacaca gcagctttcc aatcaccacc
      181 actaccaaca aatgctccga tcccgactgg tgaaggccac ggcatctgtg caagcatcgg
      241 tttgacgatt tccagccgaa tggcaaaata agcgagtgta gcagaagcca tcggagctaa
      301 gaaaaacgga accgctaaat agggattgta gacgattggc atcccgaaaa tgattggttc
      361 attgatattg aataatcctg gtacaagtga ggcttttcct aacactttca gctgatcaga
      421 tttcgcgagg aatgcaataa agatgatcaa tcctaaagtc gcacctgatc caccaacggt
      481 tacataagaa ttgttgaatt ctcctgccaa cggataattt gcgccttgtg cgttagctgc
      541 catattagct aatacgatcg gggtcaagaa agaagtgatg atattcgctc catggatccc
      601 tacgatccac aatgcatgga tcaagaaata aatcaccatg acaccaagcc atgtattcgt
      661 caactgcgta acaaacccaa atgggatagc aatcattttg aagatatccg ttcctagtgc
      721 aactaataat ccattgatga tcatcaccac aaacgcaatg acaaatgttg ggatcagagc
      781 agtgaaagaa cgagaaactc ctggcggaac aacatctggc atttttatga cccaatttct
      841 cttgacacac atacaataca attcgaccgc aataatcgac ataataatag ctgtaaagat
      901 tcctgaagtt cctaaacgtt cgacaaaaga gcccatccta ataccattta ctacagtttc
      961 tgtctcattg atagaagcaa tcaagctgat tttaccatca gtcatgatca attcagggat
     1021 acacataaag aatgcaaaaa cagatagtaa tgcccccgtg agcggattaa catttaattt
     1081 ttgttcatct cgttcaattg acgttaattc ataagcaaac acaatggcaa agtaaataga
     1141 taaaatcccc atcgtcattg tattgacgat catgtacaag tcactaactt tgaaaaaagt
     1201 tgcattaaaa aatccttcta aaaatgggaa cggtaatggc aaaatattta aaaccaaaaa
     1261 catcgagcct acaatcgtga aaggaataga agccatcccc gctgccatga cagcccgaac
     1321 gattttaagt tgcgcaattt tccccatagg tcccatcagg tatttctgca aaaaatcaaa
     1381 cattagttat tctcctttcc taaatataac gctttttcat attgtttaat acggatatac
     1441 tgttggtctt ggttgagtga aattttcttt aaacgatact gtataaatgc actggcaaaa
     1501 ataccgctta caacaagaat gaaaacaaat aactgtcctc cattgttatt tgccataaaa
     1561 ggatacaaga atgtaaatcc tgaggtaaac aacactggaa tcactaataa attaaagaca
     1621 ctctggaaca aaaaatacca ctttgtccat ggaacgttcg tttggtgttt tcgataaagc
     1681 tttacttgtt ctcctactgc aggcaaaata gctaaaatca gaatcatcgg taaactactt
     1741 cctaaacttt tagctgatag aagcagaatc atccattgca tgttcatgaa aaagaaaaag
     1801 gcagtcaggt aacgtacgat tagataacga ttgaaataca tgtttttcaa actaagttgc
     1861 tttttttcta attcgatttt cctttttact gtatcctgca caatgatcct tcctttctat
     1921 ttattttttt gatacaaata attcatttcc tgtgcaactt ccaataatgt catcgtcgtc
     1981 atcagatgat cttgtgcatg gaccatgata atttccattt ctatttttgt cccgctggca
     2041 tattctttca gaagttgagt ttgtgactga tgcgcatgaa gaatctcttc atttgcttgt
     2101 cgcaacttct cttctacttc ttcaaacatc tctttacgca tcgagcgaaa tgcttcatgt
     2161 atcagtgtcc gtgcatttcc gctatggaaa atgatatcaa aagaagtcac ttgtagtgtt
     2221 tcactgttta ttttttcagc catctgtttt cctccttatt cgatcagttt gataaattta
     2281 ctgctgaaat tctcaaaaat aggttcagct aagatttcct gctgtacttt tagctggtca
     2341 actaacctaa cgattgcttt agtcacgacc gtaattcctt cattttcgac ataagaaggc
     2401 gaaactaaaa aaacaaaatg aatttcttga tattgatccc actgcatccc agaaggaatc
     2461 aatgcaacgg ctacctttgt ttcgattcca actggaacag cgggatgtgg gactgccact
     2521 gtatcactga atatgatttg tcccatattt tcacgttgct ggatctgctt tcgcatttct
     2581 cttatatatc cttcttcttc atttatgctc agcttttgca gcaaatcatg aagaatttct
     2641 tcctttttcg gttcgtacgt ataagtcttg aaaaattttt cagacaactg ttcggtgtaa
     2701 atctgctttt tttgctgtag agaaaatgct ggtatcggtc tatctgagga atgatagggt
     2761 atagattctc caactacctt tcgtatttta cgaacatcat cttcattcaa aaaaatactg
     2821 acatgtaaaa ctggaatttt gaaaaacatt gtcgaaagat caatagaaga aataatcagg
     2881 tcgattcctt ttaaagttgt ttcattgatc tcataatagc cttttacact taccactgta
     2941 atatttttac cgaattcact gactacgcga ttctttaata gttgggcact gccgtaaccc
     3001 gttgcacaga taatcaatgc atgtagttta tgcgcagctc tttctttttc caatgccgcc
     3061 atgagatgca aagccaaata ggcccattca tcttcattga tggtatagtt tttcaaagcc
     3121 ggcatgttgc tgaaatgcct ttttactgcg tcaaagactt tgggattttc tttctttatc
     3181 tcctcagtca atgggttctc aatgcgaata cctcggctca atcgaatcat catcggtgac
     3241 aaatgatcga gtagtccatt gaaaagctgg agatcctcta ctaatgaaaa atgaaattcc
     3301 tgagacaatc ttttcagaac tttcgctaat tcctttgata gttcctgatc attttgattt
     3361 tctttgtgat tcgacttagc catgagatgt aatgtcaaat aagcaatttc ttcttcggga
     3421 aacgatacac ctgcgattac ctcaatacgt tgaacgatcc gctgcgccac ttgatactct
     3481 actcgcttag aaacttcttg ttcgatgcct aattccttga tttcaaaccc ttctcggagc
     3541 cgtttgatcc ccaaagccaa gtgtaaagtc agattctgta tgataaaatc cgaaactttt
     3601 aacttagctt ctcttgtttc atcgagaatg ataatgacta gctcttcaaa actgatgtct
     3661 gggaaaaaac ggctttgccc taaatattct ttcaatgaat ttccataatg gtttccaaaa
     3721 aaagtatcca tgataaaatg ccgtcgattt ctttcttccc cttcgatcca tgtcccttga
     3781 ccatgcttag aaacgatttt tagtgaatat ggaatcaatc gctccttgat ttcttgcatg
     3841 tcctttgtca aactggaacg actgatataa agctcttctg ccaaatcatc cataaaaaga
     3901 atggcatctt ccaaaagcaa ttggttcaat atatactttt gtcgatcttc ggactcataa
     3961 aatccttgcg gttctctagc atatccaggt actatttctt gccgactcaa aaacaagtca
     4021 aatgtcagtc tttgtaaaat atgcagttgg tagccataac ctggtttagc aatgatctta
     4081 cctccgtttt tttctactaa ctctttgatc cgctgcaagt aattacggat tgtccgatca
     4141 gataataaca attcagaagc taaacgttga ctggtgacat actccttctt gtgatcaatc
     4201 aaatatctca acagcaattt ttccttctgt ttcattatca tccactccta ttccatcata
     4261 tgttctcttc gatcacctct gctaacttct ggatgcctgt agggattggg acatacgctt
     4321 ggaaagggat actaacaaca ggctttccaa cagattctcc taattttttg aatttatcca
     4381 aaaacatcgt cgtttgcggg ctgatcagaa ataaatcaaa atcactattc ttaatcatat
     4441 tatcgccttc tgtagctgaa actgcatcta cgagtacctc ttctcttttt tgcttaaaga
     4501 aatccgttgt ctttgaggcc atcatcgagg atgacattcc tgccgcacaa ataatcaatg
     4561 cttttttcat aaatcaatca ctccttttat ttttataaac caagcatatc ttgtaagcgt
     4621 tttctattca aaatatatat ttccgtagca ttgcggaaaa aatgtgtatc atatcttgaa
     4681 taataatttc tggcaccgtg atatactagt gatagaattt tttcaggagg ggatttagtg
     4741 agtatcacag tgaatatttt gtatacaagt acaaatggac aagccagaaa atttgcagaa
     4801 gaaatggtcg aaagaaacat tgtcaaagcg attcgtgcag aagaaggaaa cgagaaatat
     4861 gactattact ttccactaga agatccagaa tctcttttac tgatcgaccg ttggaaagat
     4921 gaagaagcaa tcgaaaagca tcacaaatcc gaaatgatgg ctcaaataaa cgaactccgt
     4981 aaaaagtata aattaaaaat gaaagtcgaa cgtttcactg atttaccgta aaaagataca
     5041 catggaatga tagcaattta tcatccatgt gtatcttcac tttttcctta ttttttattc
     5101 gatctcatct aatgattcaa accgtgtttt tctcttattt ttcggataag tatttcagtc
     5161 accccaatat acatgattcc taaacatacc ccaaggatat tctcaaaaat gcggatcgtt
     5221 actgctcctg gaaccccgaa taaactggca gtgattgcca aagcaccaaa agaattaaaa
     5281 actgttttat atctataggt agaacaaaca cctaaagcta gaccacctaa tataccgacc
     5341 cagttaaaag gaataaattg gctgatacca ataaataaaa cacatccaat caatgagcca
     5401 atgattcggt ctactgctcg ttctttgaaa ccaatcgaca tcaatccata gctagataca
     5461 atagaagaaa atgcaaaccc tgcccacatc aatcgctgaa aagggacata ctctcctgca
     5521 acgatcaaaa gactaatgcc aaatgcatag atgagcatcc aaattttttc ctgcgaatac
     5581 atgcctttca aaaaaatctc tttaaacaag cttttgtctt tgttcttttc tcgatgtttt
     5641 ctgtaaagga gaactgaaaa ccaacaaaaa aacaaaaata ataagctact tgtttgtgta
     5701 aagaaattct tgttcaacga atctttaggt aacgaataaa caataaagag atatgaaaag
     5761 ccgtacaaag aagcattccc cattttggga tcactagcgg ttgccattaa tatgctgctt
     5821 agtaaaataa aatgaatcaa catgcccata actgaccatt tgatcagctg gacatacggt
     5881 gctactaata gtgagaacat gacgattgct aaacctatga tcgaatggga aatcttataa
     5941 ccaaagtcaa caaaacgaat actcatcaac atacagaata aaaccacagc gtatggttta
     6001 tgcttcgatt caaataatgt tgtaattata atgataaaac aaatggcaaa caatacgatg
     6061 aataatgatc gtaaaaacaa tgaacaaata tacatgcact tttctttttt agaagctgct
     6121 tctcgtattt tttgttttaa aataaacgga tctagttgta gagactgata aaaatccacc
     6181 tgttgtcgtc ctttcttttt acttttattt tttacagtca tctatgacag gttgtaaata
     6241 ctatggaaca tcatatggcg ttgtcatttt cttgtcaact atttttccta ttcatgtttt
     6301 tataaaaaaa catatcgttt tcttgtatga ttaaatcacc acaaaataaa caactgagag
     6361 aacgatatgc tttatgaatg gttctatcaa aaaaattctg agatcaatag acaaaaattt
     6421 gatgattaca gaggtctctt acgaaacctt acagaagaaa aaaacattga tcgttgctgc
     6481 agttctcttc cctgctccat gtgtctgtag aaattgtggt tctgctgtgg tagatggaaa
     6541 taggaaatta atggtggtga aaaacaagaa gaaggaaacc attgtccact ttgaataata
     6601 taatcatatg ccaatggtta tacgcctaaa aaagcagcgc tatacctgta aaaactgtcg
     6661 aagccattgg aacgctcaaa gttattttat ccgtccaagg cactcgattt caaatcatgt
     6721 cagacataaa atcacttctt tactgactga aaaagtgtcg ttatttttta tttcgaaaag
     6781 ttgttaggta tctttgacaa ccgttattcg cacattgaaa gagttgagaa gctatttacc
     6841 aaagtcttcc aaaagaattc tgtcttgagt gttgagggtt gtgaatttcg ttctcacgtt
     6901 tccgttgagg ataaactgag ttttatttcc gcggatggcg agacagaaaa attaatcgag
     6961 aaaatttacc tacacaaaaa attcctcgat taacaaacta tttccttagc tgcactaatc
     7021 ctaaagaaat agaattctta gttacagaca tgaacgccac ctactttcag ctcaccaaac
     7081 atgttctacc aaatgcgaaa ttggtgattg atcgattact tggtttttcc acgcctttca
     7141 aggaggcgta acctttattc acgagttggt tgaagctttc cgagacaaaa gacccgactt
     7201 gtttttctcc ttattgacaa agagccatta aaactagaga aaaaattaca actgatcgct
     7261 gattatttgt agaaagaaaa tcaaagtatt acccaataaa cttgagtcat taggcaaaaa
     7321 acactgcgaa actcaagaat gattttcgca gtgtttttgt atatttttta ttaactagta
     7381 ttactacaaa ggtttatttt tcacttctca acgcagcaca aaattgtctt tacttattta
     7441 ctggttgggt tcaaccttct ctactccgat actaatattt gtttgtatac cccttctaaa
     7501 atcggtagtt tccattcaat tgatgggtac tagttgattt taaaaactat gcttattcaa
     7561 ttttttattt tagctcatat atttcgtttc tttctaaatt ttatactgat gaaaacaagt
     7621 agcacaccca atattgacaa tataaactgg ctaatgatgg tttctccagt ttttggaaga
     7681 tgcttatttc cactgcttgt tggattgttt cctcttatat ctgagctatc agcaactttt
     7741 ctattctctt ttgttaaaaa gatcgccact tcttgttctt tttcgatctt gaaagttact
     7801 ggtgtcgcat c
//
LOCUS       DAZBSA010000114         7729 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000114 DAZBSA010000000
VERSION     DAZBSA010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.115"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..107
                     /locus_tag="ACHGMT_002450"
     CDS             <1..107
                     /locus_tag="ACHGMT_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFN6836163.1"
                     /translation="NESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(176..904)
                     /locus_tag="ACHGMT_002451"
     CDS             complement(176..904)
                     /locus_tag="ACHGMT_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFN6836164.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(917..2197)
                     /locus_tag="ACHGMT_002452"
     CDS             complement(917..2197)
                     /locus_tag="ACHGMT_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFN6836165.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2338..2940)
                     /locus_tag="ACHGMT_002453"
     CDS             complement(2338..2940)
                     /locus_tag="ACHGMT_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFN6836166.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3185..3391
                     /locus_tag="ACHGMT_002454"
     CDS             3185..3391
                     /locus_tag="ACHGMT_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836167.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3703..4422
                     /locus_tag="ACHGMT_002455"
     CDS             3703..4422
                     /locus_tag="ACHGMT_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFN6836168.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4503..5186)
                     /locus_tag="ACHGMT_002456"
     CDS             complement(4503..5186)
                     /locus_tag="ACHGMT_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFN6836169.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5405..7591
                     /locus_tag="ACHGMT_002457"
     CDS             5405..7591
                     /locus_tag="ACHGMT_002457"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFN6836170.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2774 a         1301 c         1459 g         2195 t
ORIGIN      
        1 gaaatgaaag tcaagaagta ttgctgaaag aaatggagaa ttggccaaca ttttatccag
       61 atacaatgga tggacgtgaa attgcacagg aaatgctttt gcactgatag gcaaaaaaga
      121 accgaagggt ttggatcggc tggtcattta gtcggtccaa actattcggt tctttttatg
      181 aaatcaattt ctttaatcca cggatagagg ctgggatggt ctcgccgttt gccaacgtga
      241 cttctctggt ttttttatta atcgaccgca cattttgtgt attgataaca taggatttat
      301 gacaacggat aaaattccga tgcattttct cgatttcttt gatcttctca tagaattgta
      361 attgggcatg agtcaaatgg acttccagtt tatgtggcgc tcctagcgaa gtagaaaaat
      421 attggatgtc tttgatcggt aaaaatttga tctcatcatt aaactctatt ttgaagatat
      481 ctttttggat ttgggacatt tgaaaatctt cgaaaatttt gctcaggata tgctcgacat
      541 tttcttttaa atcataaaga tcttctttga cgatataatc agtcggttcg atgttgcttt
      601 ttaagatttt catcgacatt tccgtatggg aagtcacata gattattttt cctaaaggat
      661 ctaattcacg gatcaattta ccgatcgcta caccatccat ttcactgtct tctagttcga
      721 tatctaaaaa gtaaatacca aaagaagaag ttctgatctt ggctgcattg agaagttctt
      781 ccggatcaga ggtagaaata tggacataac tgtctaagtt ttcaatcatg attctatttt
      841 tgatcaccgt tgataaaact tctaattgtt ttggatcgtc ttcacaaata taaatgggaa
      901 tcatatcatt actcctttat tttatttgta tataaatcgt ttgagaaaaa cgctggtttt
      961 caattgatgt ttcaaggaaa agataaggat tggtctttgt caattcatcc aagataaaca
     1021 agccaaggcc ttcgtttttt ggatttgttt ttgttgagaa ccctttttgt ttcaactggt
     1081 ttaacggtaa tgtatttgtt gttgtattga tcacacgaat catcacggaa tgattcttct
     1141 tgagtatggt caattcgatt tttttcttct ttgcagcact agctccttca atcgcattgt
     1201 ccaagataat accaagcata cgtacaaggt ttaccgtatg tttcgtatcc agttcgattg
     1261 gctcaggaaa atgaagctgg acgatcaacc ctttttcctg tgccatcatg agttttaatg
     1321 acagtaaact acggatctca ggaactttca tattatccaa catacttaga ttggccggaa
     1381 aaaggttcat catctggcgt gtaggtaata tcgtttgatc ataataagac cgtaatcctt
     1441 ctaagtcgcc ttctgaaata tagctgtgaa gagaaaaaag cagattttgg taatcatgtt
     1501 tgaattctct caagtcttga tagttttttt ctagatcttt gatataatcc tgttgcatcc
     1561 tgacttgttg ctctaataac tgatcagcta atttcttatt agagattttc caataataaa
     1621 ccaagatgat caggccaaca cacagatata gaatgaaaaa actcgtattc aaagctaaga
     1681 catcaggtgt ttcaccagaa aaacgggtat aaaaaataat gatatagtat gtaaataata
     1741 tcaatgtccc gatcgtaccg atcatacggc ggtccacagt gtgaaaccaa gtctctaacg
     1801 atttttttaa acaaaaagct aataaagcag ataaaaaagc ggataagaca atgtgaaaat
     1861 actgcaatga atctcctggt tcaataaccg aagatttcaa aacaaaaaaa tcgccgatgc
     1921 ttgttaagtg atctcctaaa agcgacgtca ataacccaaa aggaagcgcg taaaccaatt
     1981 gttgacgcga agtcgaaaag aagaaaaaag taagcatcag cattcctata gcaattagag
     2041 cagtaaaaat ccctaaaatc gaatagaccc atccaatcaa aatacaagaa atcagaatga
     2101 ctcccgcaaa cagatatttg tatcggatgt gatggtcaat caataagata caaattgtgt
     2161 aaatcaaata ttgcaacgct atggaccata aaaacataaa gtacagctcc cattgatttt
     2221 taccaaatta tagcacaatt ggcgtattat ttttttaatt tttcagggat tttgaattca
     2281 gaactcatac caaagcagca gccagataca aaaaagtcag ataagttagt taattgttta
     2341 atgattgttt ttttcatgaa agattcctcc aaaattcatc aagtagtttg taataggaag
     2401 caacgtagct atttctaaaa caatcccaag tagcaaatag gtagaaaagt aagaagctga
     2461 gaaaaataac gtaatgagag aaaaaattag aaacatgctg atcgccttct ttaataatgg
     2521 gggacgtttc tcgggttcaa tgggattgat tgctgtacct cttgatccat agcgaagcag
     2581 taacaaaaag cagctgatta acaaaaagac catcattggg aaggagatag tcaattgcag
     2641 atgtttcatc accaagggaa cgccccataa ataaagtagt gtccacagca gacaaacgaa
     2701 ttcagagtcg gaatgcaatc cataagcata tctgcggatg cagatataag aaagaaaggt
     2761 gattcctgtt tccgcccatg agcctgtcac cagagaaaag actagaacga ccgctagttt
     2821 tccaagatta ttcaataaaa cttctaagac aaattttact tggacataca ccatatcttc
     2881 ttccgatttg tctccgctga ataaggcttc taataatttg ttcacaactt tatctttcat
     2941 ctcatacgta cctccaccac aaattttatg ctaaaaaaaa taaaaaacgt cttttgtttc
     3001 ataaaaagtg gttttgacat caaaaccgtc atgatctcct aaaaaaatct tcgttttcca
     3061 agaaccttac caaaaaaagt gacagttttg atacgaaaag tactttttat gacaagtttg
     3121 tgctttttcg agtgatttat gttaaaaaat tactgacctt ataaaaggtg atggagggat
     3181 tcttatggaa aatgaacaat tgtttgaagt cggcgaaaca tatgcattta aatctgattt
     3241 atttgaatca ccaataaaag gaaaaataga aaaaatctat gaaaatagtg tgatgatacg
     3301 tgtcatgaaa tgcgcagtag aagacaatga taccgctcat aatctgcact ggcagttagt
     3361 tgtacgcaaa tcaagtacaa tagaagaata aaaacagata aaatgttgag agcataaaga
     3421 ggttgtgagt ctcccgttta gctctgagtt acaaggaata atgttaaaaa acagcttttc
     3481 atgttttatg aatattgcct tgtgaccgag gtgttggcag gagaatactg tttatttgaa
     3541 aaaagggctg cgacaagact tttgtcacag cctttatttt catgaatagg agttactttt
     3601 ggctactttt agagaagtag cttttaagga gcagataaag tcaaggcttt tatttctata
     3661 aatgaagtag taaaatgaag aacagatttt attacaggaa ggatgccgat aattgtgatc
     3721 attgttgcag gaacgatcgg tgcagggaaa agcacactaa ctgaaatgct tgcccaagat
     3781 ttagaaacaa aaccttttta tgagaacgtg gaagataatg aagtattgcc gctattttat
     3841 tctaatccag aaaagtatac ttttttatta cagattttct ttttgaacaa acgctttttg
     3901 gcaatcaaag atgctttcag ccatgatgac aacgtactag accgctccat ctacgaagat
     3961 agtatgctgt ttcatttgaa tgcagattta gggcgtgttt ctgaagtaga agtaaagcag
     4021 tatgaaggtc ttttagaaac gatgctgaag gagttagaag aaatttcgcc acaaaagaaa
     4081 ccagatctac tggtttatat tcgtgtatcc tttgaaacta tgttagcgag aatcaaaaaa
     4141 agaggccggg aatatgaaca gctagaacaa gatcctgaac tctattccta ctataaagaa
     4201 ctcaatcgac gatacgaaga gtggtatgaa cagtttgata tatgtcccaa aatagtgatc
     4261 gatggggaca aatatgattt cgtagccgat ccggcatgtg gagaacaaat cgtacaagaa
     4321 atcaagatgc gggcaaagaa aatgacagaa gaggcagatg cccagtcgat ccgctacact
     4381 acaaataacc cgaaagcaaa gggaatcttt acaactaaat aatataaaaa ctaagcaaat
     4441 tgctcactcg cagatatttc tctgtgagca gcatcttgct tagttttttt gattcattga
     4501 gatcaaatag tgaaccgatt cgtatgataa aattgccata aatgaaatgt ctgagtgagg
     4561 gccatgatca aaaataaaat ggaaacgatc aaaatcgaat gggcgccaaa cagatgatga
     4621 cagaaaacaa ggacaatagc tcctgcgata aaggcaagaa cgacacttag atagtggaaa
     4681 aatcgatcaa aatctgcttt ttctttagaa atacaataat catataagtt cgttgctacc
     4741 ccttttaaat ttccagttgt aaataaatta gtgtaggcac gcccgttgat tttatcaaaa
     4801 gctacccact ggatcgatgc agtaaaagca atcaaaatcg ttactaatgc gggatgctca
     4861 tggacgggtg tcaaactaac aaagaaaaaa atcactgctt cataatatag aacaaacaag
     4921 cgccagaaat gtttttcttt ttttctaaaa taatcaatta gatactttgt tagaagaatc
     4981 cctaagaaaa agaaaagggt agacaatact tttttaccaa cacttagcca ttctccgtca
     5041 aatgcttgga tgatcgatag aatgatattg ccagtttgag cagaggcaaa agcaccatat
     5101 tggatataag tataactgtc catggctcct ccgataaatg tcagcaaaag tcctattgta
     5161 cgcccctcgt gaaaggaagt ctgcattttt ttcatcacct tttatgaata tttttgtctt
     5221 ccttatctta gcataaacga ttgaaaaaaa aggaaaaatt ttcagaaaac taaaatcata
     5281 tcgcttccca ttttcatttt tagaaaggtc tgttagtata aagaaaaaat agacaacgaa
     5341 actttaaata accaacttga aataaacaaa ttcaaagaag aaagatggga aggaaatcaa
     5401 tcagttgaat gaaaaagaaa ttctccgatt acaaaaagaa ttgaaacgct gggagaacaa
     5461 gtataaaaag cttcaacaag tctttcggct acaagaagaa atcattgcta cctatgaagc
     5521 ggcttatggc gaagcacccg gacttattga ggaagaagta caagtaaaag aaagtgaagg
     5581 aaacagctct ttctttaaaa tgatggagaa acgttcctat aatcgcttca atcaagaaca
     5641 aaaagcagct atcacatatg atatgaagaa acatcttcga atcatagcag gagctgggag
     5701 cggcaaaacg caaacaatct gtgcaaaagc tgtttatctg atgacgcaaa aacaagtaga
     5761 cgaagaacgc atactaatga tcacttttac aaggaacgct gcaaatgaac taaaaaaacg
     5821 agtagataat ttcagccaga gaaaaaccgc tgtccatatc ggaacattcc atagtatctt
     5881 ttttaggata tacaatgaaa tttgtcgaaa atttcctgaa gtagcaatgc aaggaataca
     5941 aggagacttt tcaaaggata ctgcacaaaa agtgaatgct gttttgcaac agctgatacg
     6001 taaatataat ctttatatat ttgatcaata tggagaaaaa acgattgctt ctcggttaga
     6061 ctattggcag aatatgaacc tttcagtaga agaaatggtt cagttagtta aagaaaaata
     6121 tgattccatt gataagaatg cacgacagcc gatatcagaa cgactgtatg gattgctcac
     6181 ggaactccaa gaacaaaaaa gaagccagca attgctagag ttcaatgatg tactccaaaa
     6241 tctgaaaagc gctttggaga atgaggagat ccagcattat atcggccaaa aatatgatta
     6301 tctattcatt gatgaatttc aagatactaa tccacttcaa tgggaaatcg tgaaacggat
     6361 gacaaaggaa aacaggatca agttgattgt tgttggagat gatgatcaga gtatctatgg
     6421 attcagagga tcagagccag catacatcaa gaactttgaa aaagaatatc ctaccaaaac
     6481 tcttttctta ttaactaatt atcgttctcg ggcagctatt gtccaaggag caaatagatt
     6541 gatcagctat aataaaaatg atcggatacc aaaggcgatg attccagcac aaaaagaagc
     6601 aggtgttatg gaagcctatt tgttttctga tactaaagca gaaagtcagt ggctaatcaa
     6661 acaaattcag acatttattc aaaaaaatgg gaaatataag gaatcaatca tattataccg
     6721 atcagcttca caaacgcagc agcttgtcca atctctgctg aaaacgaata ttccttttgt
     6781 actggaagca gatagtccat atgaaggtat ctttggtatc aagtctttcc aacatttcta
     6841 tcaaaaaatt gttcattggc aaacagcact gaatctgcaa aagaaaaatc aggcctacca
     6901 gcaattgctc cggcagctga tggcagactg ctacctgaaa aaaagtgaat gcgatcaata
     6961 cttttctcct aaatatcaaa atatagccgt tgcagattat attttgtcga taagccccaa
     7021 tttaaaatcg aaatctgcag aaatccgaga gtttgaggaa gcgttgcgta aaacagacca
     7081 gcatcaagaa atctattcgc ttgttcaaag ctatcttcat ctgccacgta tagctaagga
     7141 aatcgaccag agtgatcaag aatggatacg agaagaagta aagcagcatg ctactttcag
     7201 aagactccaa gccttatcgc aagaaaccca agcgacagct aaagaattaa aaacccgctt
     7261 gattgcttac agacaaggaa aacttgatgc actatgtatc cagagcattc acaaaagtaa
     7321 aggtcttagt taccgaaatg tctttttgat tggttgtaac gaaggaagtt tgccttataa
     7381 cggagcgaca gaaaagaaag taatcgactt taatgaaata aaagcagaac cagtaacgac
     7441 gatagaagaa gaacgaagac tattttatgt tgcgatgact agagcaagaa atcgtctgta
     7501 tctatgtgta cctcaaatga aaaattcaag aaaattgaaa gtatcgagat ttatcaaaga
     7561 aaccgggagt cgtgtgaaaa aaggaaagtg atagaagaga gggtgtgaca gaagtggtca
     7621 gtttcaagaa agaagaagga atgtacgaaa attgcttctc aaatttttgt gaaatttcag
     7681 cttattttcg aagaagctat ttctggaaca cagtttattc ggaaaaggg
//
LOCUS       DAZBSA010000115         7559 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000115 DAZBSA010000000
VERSION     DAZBSA010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.116"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(96..386)
                     /locus_tag="ACHGMT_002458"
     CDS             complement(96..386)
                     /locus_tag="ACHGMT_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836171.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(504..764)
                     /locus_tag="ACHGMT_002459"
     CDS             complement(504..764)
                     /locus_tag="ACHGMT_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836172.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(901..1314)
                     /locus_tag="ACHGMT_002460"
     CDS             complement(901..1314)
                     /locus_tag="ACHGMT_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFN6836173.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1770..2255)
                     /locus_tag="ACHGMT_002461"
     CDS             complement(1770..2255)
                     /locus_tag="ACHGMT_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836174.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2394..2609)
                     /locus_tag="ACHGMT_002462"
     CDS             complement(2394..2609)
                     /locus_tag="ACHGMT_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFN6836175.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2833..3633
                     /locus_tag="ACHGMT_002463"
     CDS             2833..3633
                     /locus_tag="ACHGMT_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN6836176.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3652..5520
                     /locus_tag="ACHGMT_002464"
     CDS             3652..5520
                     /locus_tag="ACHGMT_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFN6836177.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5513..6004
                     /locus_tag="ACHGMT_002465"
     CDS             5513..6004
                     /locus_tag="ACHGMT_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFN6836178.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            5992..6546
                     /locus_tag="ACHGMT_002466"
     CDS             5992..6546
                     /locus_tag="ACHGMT_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFN6836179.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6564..7559
                     /locus_tag="ACHGMT_002467"
     CDS             6564..7559
                     /locus_tag="ACHGMT_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFN6836180.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2690 a         1211 c         1435 g         2223 t
ORIGIN      
        1 atttgagatc tttttatact tcaaaaaaga taacacccaa ctttcaaaaa tatgttttgg
       61 aagttgggac aaacgagtta atacatttta atacattatt ttttattcaa aaaaaatagc
      121 acaatggcaa agatcgtaag agagatcata agccccaatg aatgactaaa aagaaaagca
      181 gaatcacatg ccatatcatc ccatatttct tcaagaaaat aaataagcgg gaaaaaggga
      241 aggaatagta gaacatcgat ccagcaccat ttttttcttc ttttgaaata acataaaaca
      301 ataaacatga acgtgcaggg aagtaaaaac aaagctttac tcataactaa caaaatacca
      361 gctcctttta tttttttctc ccccacattc aaagagtaac aatggtatgt acttaataat
      421 agaaaaatgg atcaacttgt atgaataaca gctgaaaaga tctgcaccaa ataaaaaact
      481 tccagccaaa actgactgga agtttatttt tttctgaaat attctaacac aaatatgaaa
      541 aagatggccg aagctaataa gccgatttcg tagcaaaaca atgagatacc agagtcgtaa
      601 taataaaatc ctctaatctt atggataaaa tagatcatcg gacaagcaga taagagtaag
      661 tatttgttag gccttttgaa taagtaaaag aaagcactgg tcattatgat aaatggtatc
      721 caaaacaaaa gtttacttaa gacaatcaaa tgatcaactc ccatattttt taccaagaga
      781 gatcataaca tctttatcgt aaaaacaaaa tatttttttg ctcattttga aaatttaaac
      841 atactcgttc ttatccaaat atataaaaat cgcccctcat tcatcgagag gcgataagtt
      901 ctaatttaac tcaatttttt ttgtttcttt tggctgataa gctgttttag gtaataccaa
      961 tttcagcaca ccgtttttaa aggttccagt gattttttct tcatcaacat taggaagata
     1021 gaatgaacgt ttgtaagaga ccgaatgtct ttctttccgt acatagttgc catcttcttt
     1081 atcttctgta tgggactcat gattggcgga gatcgtcaaa gtattatctg aatattcaac
     1141 tgtcgtgtct tctttgtcac agccaggcat atcagcagtt agtttgtatt ctttttcatt
     1201 ttcgactaaa tctacttttg gataacttga aacacctaaa aaatcattga atgctggact
     1261 aaacacgtca ttgaaatcag ggaacatgtc gcgaatgcta ggtacatttg ccatttttga
     1321 tcatcctttc aataagtatt ttttatttat ccctatttac gtaataactc tattttgaaa
     1381 aaaatgcaag tataatggtt agctttgtga atatttcatc taatacgata caaaggctat
     1441 ctttctaatt tgaaaaaggg atcattaaaa taataagaag aaaatatgtc aaaaggtaaa
     1501 aaagtaaatt cttctatcca aatatagaaa aattgaatct agaaaaccgc ccgacaataa
     1561 atttcattaa ctcagttgac cgaaaatgaa gtagatacca aaaatttatc aagagagcaa
     1621 gtagagaagt gagtatattc ataagagaaa tagctgtaac gaattattag gttaatgcaa
     1681 atgcttattt atactccgta taactttctc agaaaaaatg ataccaataa tgacttgcta
     1741 gtactgtaaa tccgtataat atcatctaat caaaatggtg ttccatttgc atctgtcatc
     1801 gtaatcgatc cttcgggtga cactcgataa aaaccaacgc ttccagatcc tccttgggaa
     1861 cggatttgtt tgctggaagc tgtgaacaaa taatctcgat caattttttg ataaaagcta
     1921 ataacaatat cgtcactttg taattcggaa cggtgggcaa taagaaatag aagtgctttg
     1981 tcagagtttt ctaatggaag agaaggtaca gtgctttctt gcatttcaga tgttgaagaa
     2041 atgctaaacg actgaatact tgattggcta tgttgcgaat ctacattacc agtagaagta
     2101 ttagtaatgg aagtagtaga agtactttta gttgaaccaa cctcagttgt tgaaatggct
     2161 gctgaagatt ctttaacaga attctttgtt ttatatccac aggcagaaaa aagtattaca
     2221 gttcccaaca agcctgcgta aatcccttta tgcatgatag ctcctcctaa aatagattat
     2281 ctttattcag tatatcactt aaacaaagaa aagcagatgg tttaaaatga aaaaaccttc
     2341 ccaataaagg gaaggttcat gaagataata acttttttaa taattatagc tttctaatat
     2401 ctttcatcaa cttttctagt aatcattggg aaaaatttca agcagtagat cgcacaaata
     2461 cctacgacga tataaatgat cttcgcaaaa atagttgttg aaccaccagc aatcatagct
     2521 actaaatcaa gttcgaacaa gcctaccaac agccagttca accctcccac aatcaataaa
     2581 gttaatgcaa tagcatctaa agttttcatt agaattctcc tttcttaaat gataaaacaa
     2641 ttatatatca aaaagagaaa cattgcttat ataacgctca ggaattttag caatctatgc
     2701 aaaaagatga aaattttttt tgctcttcca ttagtacaca ttttttagct gaagctgtaa
     2761 atttcatgtc ttaatttttt ttagtgagct tttatactgg atttgtaaga aaaaagaagg
     2821 agaggatcac atatgtcgga ttggttggat attgcaggaa aagtagtaat tgtaactgga
     2881 ggttcctcag gaattggaag aagtatcgtc gaaaatttgt tgaagcagaa tgcacaagta
     2941 gcgaattttg atgtaactga atgccgtata cagcatgaaa acctactatc tttaaaagtc
     3001 gatgtgtctt ctaaaacgga catagaagaa gggatatata aagtaatgaa acatttcaag
     3061 acgatcgatg gtctagtcaa taatgcagga ataaatattc cttctcttct aatagataga
     3121 aatcatccaa aaagcaagta tgaattatca gaacaagttt ttgataagat gatcgctgtc
     3181 aatcaaaaaa gtgtatattt gatgagccaa gcagttggaa gaatcttggt gcaaaaggga
     3241 tcaggagtga tcgtgaatct ttcttctgaa tcaggattgg aaggatcaga aggacagagc
     3301 tgttatgctg ctacaaaagc agcgatgaac agttttactc gttcttgggc aaaagagtta
     3361 ggaaagagga atgttcgtgt ggtaggtgtt gcaccaggga tactggaaga aaccggtttg
     3421 agaacacaag aatatgaaga agctctttct tatacgagag gtataagcgt cgaacagctg
     3481 agaaacggtt acagtcgaac aagtacgatc cctctaggaa ggagtggtaa gctgcaagaa
     3541 gtggcggatc tagtctgcta ttatctttca gaaagatcaa gttatatcac tggagtcacg
     3601 actaatattt caggaggtaa aacaagagga taaaggatag gggggacgca cgtgacgcta
     3661 gtaaacagat ggtaccaatt attgaaatta ctagtcactc acaagaaagt ttcaatccag
     3721 gaagtgcaaa aagaaatcaa aaccagtaat caaaccataa aaaaagatat tggggagttg
     3781 aatgaagaaa ttgccggtat tgcaaaaatc atccaaaaaa acaaacattt tgaacttgaa
     3841 atttatgatt ttgatttttt tgatgagatc atgcaaggaa aattgaaaac agccagtgat
     3901 ttcaattcgg catccaaaag aatcgcttat ttactaaata ggctgatcga aacagaggat
     3961 ttcttacgta tggatgattt gtctgaagaa gtcggtgtca gcagaggaac agtggtcaag
     4021 gatctaaaga cattaaaaga gatgatcaat gattttgatg taaagattac aggcacacca
     4081 aataaaggac taagaataat aggagatgag ctagaattac gactactttt attttatttt
     4141 gtttctccct acttttcaga gacgaaaaca gaacagtttc tagaagaaaa acaagcaatc
     4201 aaacattttc agaagaagac gaatgctagt aaacaagaaa tcaccctttt aacgaaagtt
     4261 atcggtattt ctttgaatag aatctctagt aactatctgt tgaagaagcc catcaatcgt
     4321 tacagtgggt gttttgaata tatccaagaa ttcaatgaac tgatcaatca gttggaagaa
     4381 atctatgaac taactttgag ccaatatgaa aaagatttta tcagttttcc tttgaacatc
     4441 atcaacacaa gtataacgat gataaggaaa gctaactctg atttgcgctt atatttcgac
     4501 cccatgatga tgcgtattcg acgattagat ctcattgatc tggatgagtc cttcttatat
     4561 caagagatga aacagcacct ctctttattg ataaatcggg taatttttcg ttatcgattg
     4621 actgatttat tttatggtga gatagaaaaa aagtatcctt tttcttacca aatcgctagc
     4681 gaatgcatac aagcattaga agctatcttg caacataaga tctctaaagt agagatcagc
     4741 tacctaacgt tgtatttgga gttagcattt cgatatcaaa aagagcattt ttcctctaaa
     4801 aaagtagcca ttgttcatac gacaggaaaa ggaacagcat taatgatcca aagacaaatc
     4861 aaaaggatat tggggaggga tatacaaata acacatgttt cagaagaaca atatgaaaaa
     4921 gtagatctaa acaaatattt cgctgttttt acaacgattc cgttaaaaaa tgtaaatgaa
     4981 gagacaccgg tgatccattt gactaatctt tttgatgact cttggttaag ggcacaatgg
     5041 caaaaagcaa aaaaaaagcg attagccggg tttcattcta ctgttttttt ctatcaaata
     5101 tggaaagaaa aaaagacgta tcaagaatat ttaaacaaat tagtcgctcc tttgatcaga
     5161 ggctattatg tggatgaaga atttgaaaaa aggatcttga aaagagaata tcagcaaagc
     5221 acatttgtta acaaagaatt tgcttttcct cacacaacca accagctatc tcaacacatt
     5281 gttttatcag tagggctaat aactgatcaa ggtttagcag aaacaggagg aaatggagtt
     5341 atttttttat ttgccatacc tgaagaaatg acggctgaag ttgagaatga attatttgag
     5401 ctatacgata tcctatttga actcataaaa atgggaatag atgagtttat tgaattagca
     5461 ccgacacaag aatcattttt aaaactaatc aaaagtaaag gagagagact tcatgaatga
     5521 tatgtatcta ttcatagttt tcgcagcttc agcttatgtg ctgcaagcag ttatcagttg
     5581 gaagcaacta aagcatttca atcaaacata tagcgaactg cgaagaaaag gaaaagtagc
     5641 gattggtaaa cgacctggga aaatccgttc aggcacgatt gttatgctag ctatagacga
     5701 tcatgatcaa gttttagaag tcagaaaaat gcaaggggtt acatctttgg caaaatttca
     5761 ttcattacct gtctatgcgg gtgaagatat ccattacatc gatcgttacc atccctcagt
     5821 tcgagcggaa gatcgtttga cacaagcagc tttagaaaat gccagagaag tatacttacg
     5881 aatagaggca ggaaatgaaa aaagtgaaca accttcattg ccttttattg ctatgctcaa
     5941 taattttcgg atatggaaaa gtacgatcaa agaaaaaagg agtgacaaac gatggatatc
     6001 ataacaaaat ttgctgaggg atttatgagg ctgttccaaa caggtgcaga aacattcata
     6061 agctggatga caggaatcgt accagttgtc ttgatgctga tggttgcaat gaatacgctg
     6121 attgcttttt taggtgaaga ccgagtaacg aaagtagcca aattatcagc gaaaaatccg
     6181 cttactcgtt accttgtgtt accgttctta tctgctttta tgttgggaaa tcctatgtca
     6241 tttacaatgg ctcgtttcct tccagaatac tacaaaccaa gctattatgc tgctcaagct
     6301 caattttgtc atacgagcaa tggtgtattc ccccatatta atcctggaga attgtttgta
     6361 tggatgggga ttgctcaagg gatcgaaacc ttagggctaa attcgatgga gctggctatc
     6421 cgatacttgc ttgtgggact gctgatgaac tttattggtg gttggataac tgattttact
     6481 actggatttg tatgcagaca gcaaggaatc gttcttagca aaaaagtaga gctttcagtt
     6541 gattagagaa aggagagttt taaatgtatg gtgatattaa gatcgtaaga ggaaacggcg
     6601 ggtatggagg tcctttgatt attaggccga caaaagataa acacaagttc atctatgtca
     6661 caggtggagg tgagaggcca atgatcgtag ataaaataga agaactgaca ggtatggagt
     6721 ctatcaatgg gttcaaaaca tcaatacctg atagtgaaat cgcgcttgca atcgttgact
     6781 gtggagggac tcttagatgt ggaatttatc ctaaaaaagg gataccaacc atcaatatcg
     6841 tttccacagg aaaaagtggc ccattagcac aatatatcac tgaagatatt tatgtttcag
     6901 cagtagggtt agaacaaata cagccactaa tggaaactga tgataacaaa gaaacagtaa
     6961 atactgcatc tccagataaa aagtacaaat atgatacttc taaaaagata accgagcaaa
     7021 aagcagagca gcaaaactgg atagcaaaaa ttggaatggg cgcgggaaaa gtagttgcta
     7081 cattcaatca atcagcaaga gaagcagtag atacgatgat ccggacagtg atccctttta
     7141 tggctttcgt ttcccttttg attggtgtga tacaaggttc tgggatcggt aatgtatttg
     7201 ctaacattat ggcaccttta gcaggaaatg cagttggatt aacaatcatt ggctttattt
     7261 gttcactgcc atttttatct cctttgcttg gtccaggggc agttattagc caagtgattg
     7321 gaacattgat cggtgtagag attgggaaag gaaatattcc tccccaatta gcattaccgg
     7381 cactatttgc tatcaatact caaaatgcat gtgattttat tccagtcggt ctaggtttgg
     7441 aagaagcaaa agctgagaca gtagaagtag gtgtaccatc agttctttat tcaagatttc
     7501 ttaacggcat cccacgagtt atcattgctt gggtagcaag ttttggatta tatagctga
//
LOCUS       DAZBSA010000116         7323 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000116 DAZBSA010000000
VERSION     DAZBSA010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.117"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..546
                     /locus_tag="ACHGMT_002468"
     CDS             <1..546
                     /locus_tag="ACHGMT_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN6836181.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSLVQFRKTTVTVV"
     gene            735..1403
                     /locus_tag="ACHGMT_002469"
     CDS             735..1403
                     /locus_tag="ACHGMT_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFN6836182.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1603..1923)
                     /locus_tag="ACHGMT_002470"
     CDS             complement(1603..1923)
                     /locus_tag="ACHGMT_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836183.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(1960..3342)
                     /locus_tag="ACHGMT_002471"
     CDS             complement(1960..3342)
                     /locus_tag="ACHGMT_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFN6836184.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(3498..4685)
                     /locus_tag="ACHGMT_002472"
     CDS             complement(3498..4685)
                     /locus_tag="ACHGMT_002472"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFN6836185.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4692..5723)
                     /locus_tag="ACHGMT_002473"
     CDS             complement(4692..5723)
                     /locus_tag="ACHGMT_002473"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFN6836186.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5769..7193)
                     /locus_tag="ACHGMT_002474"
     CDS             complement(5769..7193)
                     /locus_tag="ACHGMT_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFN6836187.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
BASE COUNT         2188 a         1466 c         1394 g         2275 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg gcgtatcaaa tgaaagctta tagaaacaaa
      301 ttgaaggagt acaaaatctt tcaaagcatg tctcggaaag ggaactgctt agataattca
      361 cttatggaga atttttttgg cttattaaaa caagagattt ttcacgggaa agtttataac
      421 tgtttcgagg aattaaaatc agccatcgat agctatatct attactacaa taatgaacgg
      481 ataaaacaga aattaaactg gcaaagtctc gtccaatttc gtaaaacaac cgtaacggtt
      541 gtgtaaaaat atcattttag agcataaaaa aaaggagtgg atttctccac tccaaaaaag
      601 tctaactttt gggggtcact acagtgtcac agtccttttt ttagtaaaag gatacatttc
      661 ttccttgtat attccatgcg tgttcgtgag aaagcatata taatttaatt aacagatagt
      721 tagggggaga aaaaatgatt ttttcaatta caatgaaact ggtgttcggg ttgatcggtt
      781 tattattagt ggtacgtttg cttgggaaga aatccatgtc agaaatcaca ccgtttgatt
      841 tagtctatac gcttgtttta ggtgggatct tagaagaatc aacctatgat gacaatgtcc
      901 atgtagggca tgtcctattt gccattgcgc tttgggcagt aatgatttat ggtatcgaac
      961 gtatcgtcca aaaaaatgaa aaggtcaatc gttgggtcaa aggcgagcca tctgttctaa
     1021 tcaaagacgg cgtcatcaac atgacagagt tgacaaataa ccatatcgag atggagcagt
     1081 tgcgagcaat cttaagacag caagaatgct tttttcttga aaatgccaaa catgttatcc
     1141 tagagaatgc cggtcaaatg agtgtattga aaaaatcaga tgaagacaaa gctttatcga
     1201 tcctgcttgt agatgaaggg caaattcagc ataaagtgct tcagtccaat caattgactg
     1261 aagcatggct tatggagaac ttgaagaaag aaggctatgc agatgtaaaa cagctgattt
     1321 atgttgaatg gtcagaagaa aaagggctct acatactaaa taaagaagac gtcatgcatg
     1381 aatcttaccg aatagatgga tagatagaca gaggttgtga aatcagcgcc ttggcttgtt
     1441 actcaaaact aagcgacttt ttcacaacct ctgatatcta atatacgttg ttccagaagt
     1501 tgcttcttcg aaaaataagc caaaattcca caaaaattta agaagcaatt ttcgaaaaat
     1561 cctttcttat tttttgaagt tgatcacttc tgtcacaacc tctcatgtca caaaccattt
     1621 ttttgtttcc tttaactcaa tataccgtac gtcttcccac aatatcggct gatggtcaat
     1681 cataaagtaa tcttcatagg cttcaccaat gaagtgccct tcgacctcat ccaagagtct
     1741 tccaaattca tcttttaaat tgagctgaat gattactgct ttacgagata aaaaggcttc
     1801 tgtcagtata ctatcgattt cttgcaatgt catttgttgt ttaggaagag gctgtttgcg
     1861 tgcttcttct tcattttttt gataccgttg actagttctt ccatcgcaaa agcagtcccc
     1921 cattttaatc caaatggccg atctcgatac tcgttatagt caatgaattt ttttgctgtc
     1981 cgtctcatct tttccacctt ctaatccact cataccacct gcatgcccgc caacgagaga
     2041 actacgtgcg atggcgcgtc ctccttgcaa gagactatgc gcatggatga ttgattgaaa
     2101 gccgtatttt tttctaatcg tatcgacaac aaaatcaact ttcgcttcat ttatctgctt
     2161 ttcaggatct tcaaaaaggt taagctgtaa acctgtcgcg tatacgaggt cggaacagtt
     2221 cacgcctaca cgccgaatat cctggtagtg gtaatgctgg tggaaaatgg tcaataaagc
     2281 atctgcaaga tctctgctag catttgtcgc ttcgattttc atttgctggt gaaacccggt
     2341 cttgccattt ggatcaagat agcctaaaga gtatcctacc cataagctga ctacttgtgt
     2401 ttttgctgca gatcgtctca agcgagtgcc gacttggtcg gccatttctt tgataacgat
     2461 ctcgatttct tctcgtcttg tataatctct atctaggatt tgactatttc cgatactttt
     2521 tgattttggt gtatattctt gtcccaaaaa acttcgatcg atgccccaag catgggcata
     2581 aagctgagta ccgatgaccc ctagcttgct tttcaagtga taataatccg cgtgagctaa
     2641 atcataaaca gaatgtatac ccatttgttt cagacgtttt gctgttcgtt tgccgattcc
     2701 ccaaaagtca gtgatatctg agattttcca taacttttct ggtacatctt cataacgcca
     2761 ttcagcaaac atttgctttc cgtcttttga gccattgtct aaggctagtt tagcaagtag
     2821 cgggttatcg ccaattccta tagtcgtgta gattccggtc tgctcaaaca catctttatg
     2881 gatcagttta gccatgtcgt acgcatttgt tttaccaaag agtgccatgc tatttgttac
     2941 atcaaggaag ctttcatcga cggaatagac atgatggttc gcttcgtctg catatttttt
     3001 atagatagaa ttgatttctc tgttcttttc catataatag gccatccttg gggcaacaat
     3061 atataaatca cttggatagg ggaagggaag atctctggca cggctgatat tggtgatgcc
     3121 gtaagctttt tttgctgcag gactggaagc caagattaat ccgctacccc gtgtttctgt
     3181 cgaatcggac ggataggaca tgactactaa tttagttgta aggggatcaa gatccttttc
     3241 aacgcactct acggaggcgt aaaaagattt gcagtcaata cataaaatat ctcttgaagg
     3301 ttctttttca taaacaaata ccggattttg attcgcccac ataacaatca cctcttcaac
     3361 acaattagaa cacacgttct ctttccgtgt caataaaaaa cagcaatttt caaatacgag
     3421 caaaaggctc acatttggaa attgctgaga ggttattttt atctatttgt ttttagctga
     3481 gatcgttgat ttttttatca aacatgaaca gcttctggat cgatctttgt tgtcccattt
     3541 ccaatgtgtg caacataaaa ttccggagca tagccgacaa ctttcatgta ttcatcatag
     3601 acattgtttt tgaaggcagg tatattttct tctttgacaa gagcaatcgc acagccacca
     3661 aatccagctc ctgtcattcg tgcacctaag acaccttctt gtttttgagc agcatctact
     3721 agtgtatcta attcgatccc agttacttca taatcatagc gaagagagtg gtgagaatca
     3781 tttaacagtt gaccaaatgc ttctaagttc ccagcttcta attcagcttt tgccttcaaa
     3841 gtccgctgat tttcagaaac ggcatggcga gctctgcgta tcaatgtatc atcgccgatt
     3901 tggtcaatag ttgattcaaa ttcttcttca tctaagtctc ctaaagaaga gataggcaaa
     3961 gttttttgca aacgtgccaa ggcttcttcg cattcgctac ggcgttcatt gtattttgaa
     4021 tctgccaatt cccgccgctt attcgtattc atgatgacga ttgcataacc atctagtttc
     4081 actggaacca tttcgtattt cagcgtattt gtatctaata agattgcatg atctagctca
     4141 ccgaagccga tggcaaactg atccatgata cctgaattga caccgatgaa ttcattttct
     4201 acttttttac ctgtttggac tagttccaag cgatctgttt ttaaatcaaa cagatcttct
     4261 aatacaacac ctactaataa ttctaatgaa gcgctactgg aaagtcctgc accattaggg
     4321 attgttcctt ccactaataa ctcaaaccca tgatcgattg agtgtccagc ttcttttaat
     4381 ttaaggacca ttccttttac gtaatttgcc cagtttgctt ttttatcata ttctagctca
     4441 tctaaagtaa aactaatgac accgatatcg tcaaagtttg tggaataaac taatatttta
     4501 ttatcgtctc gaggtgctgc tacaccttgc gttccataag tgatggaagc ggggaagaca
     4561 tggcctccat tgtaatctgt gtgttcacca atcaaattga tgcgtcctgg tgagaagtat
     4621 tgtgtagttt ttttaggtcc gaatgtttcg gcaaattttg tgtttaagct tcttttgatt
     4681 tccatgatga gttattcctt tcttgttttg attttgaatt ctgttactgt ttcaaatact
     4741 gatccggctt ttaaatgaat cgaaccaaat gctgagaagc gctctgcacc tggagctgtt
     4801 tgagtctcaa atgtgatgcc accgtgatgt gccagttttc ggttgcgcat ttctggtgtt
     4861 tctgtgccga aatttgcagt gaaaataacc acactagaag catctgtagc aatatctact
     4921 tggattcttt tatcggggct agtcaagcga gcggcttctt tacccaagcc ggtttctttt
     4981 aagaaaaatg gatgatcgat tccgtcaaca agattttttt gatccaaatc actagcaaaa
     5041 acggacgcta attttttagg tatttgaaaa tcgaaagcac ttcctgcagc attttctttt
     5101 acccctatag gaatactatc tgttcgcaaa ggagcataag cttcgctatt gagccaaagc
     5161 tcatgttgat cgattgactg agaagcatct cccgtcaagt tgaagtaaac atgatttgtc
     5221 ggattgaata aagtatcttg gtcactggtg ccgcgagagg tcactcgcca gatattatct
     5281 tttgtcaaag tatagcggat ttccacatcc attgtacctg gaaaaccatg ttctccgtcg
     5341 ggacttgtag tagtaaaaat gacagaagct tcattttcgc cattcaaaat gacataagac
     5401 cattttttta attcaaatcc aggagcacca ccatgcagat tatgtccagt ttttggatct
     5461 gtggctaatt ggtaagtttt tccattgaga gaaaagcggc cattttcgat tctccctgcg
     5521 gtgcgaccga tagaagcacc gatatacgga tctttttcga tatattcttc agcggtgtca
     5581 aatcccaaaa ctaattctcg atcatttgat tttaaactga cgattctagc acctaaatca
     5641 gagatagaaa gtgctagctt gtttttgttc tccaaggtta tcaagtgata accttggcca
     5701 aaatcttttt ctgtaatctt cattttcaag atcctttctt aatgggaagg aactactgca
     5761 tcgtgaagtt aatcttccat tgtatcagct ggagccaatt tgttttccaa tttctcaacg
     5821 atttgtgcat gttttttctc tgtcaatgta atcttccaag caaaaataat agcggagaaa
     5881 atcatcaaag cagcaggagc atagaatgca taagctttga aagtcgttac gccatgagca
     5941 gtgatatcag cagcagtagc attaccagtc atcccagaag cgatagcgac aaaccctacg
     6001 atcccgttag acaaagcacc agcgatttta tctagtaaag ggcgaattga tagagtaaca
     6061 gcttcgttac ggataccatt tttccattga ccatattcaa cagagtctgt gatcgtcatc
     6121 aaagcggata agaaaatcaa ttgttgaggg aagtagaaca atactagccc gatgataacg
     6181 attggcaaac ttgaacctgc gatcgtaaag aaaaagtaag aagaaatcat cattgcgatc
     6241 cctccaaggt aaacgtagcg acgagagatg acgcgtgtaa gcattgggaa tagtggaaca
     6301 gctactaaac caagtaaggc agcaactaga ccaacgatcc aaaattcagt tggttggcca
     6361 agtacatatt tgaagaagta gaataatacg gcagttgttg ctgcattggc aatcgcataa
     6421 gcgatataag caagggcaag ccacatcaac tgatcatttt gagcaactgc tttgaacaca
     6481 tctttgatcc cagctttttc attttgctga cggatcgcag tatcgacttc ttttgtgccc
     6541 aaggcagtga taatcgcagt accgcctgaa ataacagcag caatcacacc gaaccagaac
     6601 cagcctgaag cgccttcaat atgtttatga gtaacaagat aggtaaagaa agtaacgata
     6661 gggacaacga ctagagtagt accatttgca cctagagaag agccgaaacg agcaaacgta
     6721 gcaaattttt cacgttcctt tgtttccgta ctcaaagctg gaatcattga ccaaaaagcg
     6781 atatccttaa atgaataaaa actatccaat aagatgaaaa cgattgcaaa taatatgata
     6841 aataaagtcg tattatgagt agctaagcca aagaaattgg taaagattgc taccaagagt
     6901 acggaactaa caacgccgcc gataactaac catggtttga atttaccgta ttttgtacga
     6961 gtattatcga tgatgccacc aatcaaagga tcaaaaataa tttcaactaa gcggatacct
     7021 acaactaagc ttgtaactgt agcaatcatt tttgcattcg ttgccttatc tgttcctgcg
     7081 aacatctggc ttgtcacgaa taccataaaa taagtgctta atgtaacata gaatgtgtcg
     7141 tgtccaaaag cgcctaaagc gtaggacaca cgttggatca ttttgctttt cataatctgt
     7201 attcctctcc tgttcaatat ataatactaa gtaaaccggt tacaacgaat aacaaagatc
     7261 ttttaatgac ttaaacttaa caagaaatcg cttccttttt aatatatttc tgtgactttt
     7321 att
//
LOCUS       DAZBSA010000117         7304 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000117 DAZBSA010000000
VERSION     DAZBSA010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.118"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..829)
                     /locus_tag="ACHGMT_002475"
     CDS             complement(<1..829)
                     /locus_tag="ACHGMT_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFN6836188.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     "
     gene            complement(841..1794)
                     /locus_tag="ACHGMT_002476"
     CDS             complement(841..1794)
                     /locus_tag="ACHGMT_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6836189.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(1784..2854)
                     /locus_tag="ACHGMT_002477"
     CDS             complement(1784..2854)
                     /locus_tag="ACHGMT_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6836190.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(2860..3903)
                     /locus_tag="ACHGMT_002478"
     CDS             complement(2860..3903)
                     /locus_tag="ACHGMT_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6836191.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(3904..4845)
                     /gene="opp3b"
                     /locus_tag="ACHGMT_002479"
     CDS             complement(3904..4845)
                     /gene="opp3b"
                     /locus_tag="ACHGMT_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFN6836192.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            4913..5275
                     /locus_tag="ACHGMT_002480"
     CDS             4913..5275
                     /locus_tag="ACHGMT_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFN6836193.1"
                     /translation="MKEQSNEKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFL
                     WSLPFLIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLII
                     AGILIALSAIFMLFSLLG"
     gene            complement(5345..7006)
                     /locus_tag="ACHGMT_002481"
     CDS             complement(5345..7006)
                     /locus_tag="ACHGMT_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN6836194.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            7185..7304
                     /locus_tag="ACHGMT_002482"
     CDS             7185..7304
                     /locus_tag="ACHGMT_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFN6836195.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
BASE COUNT         2044 a         1515 c         1335 g         2410 t
ORIGIN      
        1 cttcttttgg aaaagattct acaaataagt agtcatattg tttgaccaat ttcccagata
       61 attgttcaag ataatcttct cgctggttct tttgtttcag ataaagatcc attacttttc
      121 tcttttgttt ctgataattg cttgcctgat ctaacctgat ctttctatgg tgagcggagc
      181 ggactttcac attcaatttc cgctgtgcat gttgcagctt ttcttctgtc tggtgaaatt
      241 ttggtaaagt tacttctaga ggtcgagatg tttggatcaa tgtcttcgga tgataggcga
      301 tcccgatcca tttagctaaa gattcttttt ttactggcga aacatcacaa agtagtgcga
      361 cataaaattc ttcattatat cgagcagaaa tcgtagcgga gcgaatcgat ccttcaactg
      421 gccgatgctg atttatcttg attagttctt tttgttttgg taactttaag tagccatttt
      481 ccagatagat cgtacgggtt tggttgttgg tcgtataaga ttgccacata ctttttttcg
      541 ttttaagctt tgggaagctg gcacgtccct tgaaataatt gcgaaaagcc cgatccaaat
      601 taagctgtgc atttgctaaa gcaagactgt ctacttcttt taagaatggg tattcttttt
      661 ttaatgtagc gggtgtgagc gaaaaattgg ctactcctgt ttgttgccaa cttttctgcc
      721 gctttttcag caaatggtta taggtaaatc gagcacatcc aaaagtacga atcaattgct
      781 gtttttgttc ttctgtcgga tacatacgga atttaaaagc ttttagcatc tatgtccctc
      841 ctagttagca accagaagag attccttttg tcgttttagt ttttttgcgt aatatgggac
      901 ttcctgctca gttgcataga caaaatgttc tggtgcgatt tcacgaagtt gacgtacttg
      961 tccgtcatct ggttcaggtt gatatttgat tcttctgcgt tttctctcat ggtctggatc
     1021 tggtagagga atagcagata acaaactttc tgtataagga tgaacaccgt gattatagat
     1081 ttcatcactt gttcccatct ctaataacaa gccgttatgc attacaccga ttcggtcact
     1141 aatatgttta accatcgaca aatcatgtgc aataaataga taggtcaatt gatgttcttt
     1201 ttggagatct tgcaacaaat tgaccacttg ggcttggatc gacacatcta gtgcagaaat
     1261 tggttcatca caaatgataa aacgcggttt tactgccaat gctcgtgcaa taccaatacg
     1321 ctggcgttgc ccacctgaaa attcatgagg ataacgagta ccgtggcttg gattcaatcc
     1381 aacagttctc aatagatcat cgactttttt tgcacgctct tcagatgatt tagctagtcc
     1441 attaacatca attccttcgg caatgatatc tctgactttc atacgaggat tcaacgaagc
     1501 gtatggatct tgaaagatca tctgaacatc atgccggaat ttcgtcaaac cttttttatc
     1561 tttgatcttc gttatatctt gaccgtcgaa taatatctca ccatcagtag gttcattcaa
     1621 gcgaatgatc gtacggccag tagtggattt tccgcttcca gattctccaa ctaaaccgaa
     1681 tgtttctcct tcataaatgt gaaagctgat gtcatttaca gcatgtactt cattcttttt
     1741 tcctacattg aaatactgtt tcaatccttt aacttcaagt agtttatttg gcatcagcaa
     1801 ttcctccttg accgtaagta tgtttttgtt tctctttaaa gatagcccag cgacgttgga
     1861 tttcttctgg cggtgtcact ttgggtgcct gcggagcaag tagccaagtt gctgccttgt
     1921 gtgtttttga tagttcaaaa aatggtggtt cttgttctgc atcgattttc atcgcgaatt
     1981 catttcttgg atagaatgcg tctccttttg gcgggttcaa taaatccggt ggagaaccag
     2041 gaatagcata caatcgatcg ttcgcactat ctaatgtcgg cattgaacca agcagtcccc
     2101 aagtataggg atgttgcggg ttgtagaaaa tttcttccga tgttccaact tcaacgattc
     2161 ttcctccata catgacagcg acacgatcag cgacattcgc tactacacca aggtcatgag
     2221 tgataaagat aattgatgta tcgatttttg tctgaatttc tttcagcagc tccaaaatct
     2281 gtgcttgaat agtcacatca agcgctgttg taggttcatc agcaatcagg atttccggat
     2341 aacataccag tgcaatcgcg atcacgatcc gttgacgctg acctcctgag aactgatgcg
     2401 gataactgtt gatacgtttc tcagcttcgg gtattccaac taatttaagc aattctaatg
     2461 cagctttttg tccctctttt tttgaaactt tattatgctt tctaagggac tcagcgactt
     2521 gcttcccaat gggcatcgtc ggatctaaag aggtcattgg atcttggaaa atcatcgcta
     2581 tttctttacc acggattttt tgcatctctc tttctgattt tccaacgata tcgttacctt
     2641 taaagaggat ttgaccagca tctatattgg cgttgcttga cagcaagcgc atgatgcttc
     2701 tggtcgtcac tgattttcca cttccagatt ccccaacgat cgccaatgtt tctcctttat
     2761 tcaaatgaaa attgacaccg cgaatcgctc gtacctttcc agcatacgta tcaaaagaga
     2821 tttctaagtc tttcacttct aaaattttcg tcatcgtctt cactctttca ttttaggatc
     2881 aaaggcatca cgcaatccat cagcaagtaa gttaaagcca atcatgataa ccgataaagt
     2941 tactgccgga atccataata gataaggcag atacaggaat gtcttgtacc cttcgctcaa
     3001 catcgttcct aatgaagctg acggaggcgt aagacctaaa ccgataaagc ttaaaaaggc
     3061 ttcgaagaaa atagcagatg gaatactaaa catcatttga ataataatga cactagagat
     3121 attcggcaaa atatgtttga atgcaatttt cattcttgat tcacctaatg tcaatccagc
     3181 taaaacatat tcttgatctt ttaatttcat tgtttgtgca cgaacgatac gtgccattgg
     3241 tatccagttt gtgatggcca tagcagccac gattgaaaaa attcctggtt caaaaacgac
     3301 caacatcaaa atcatgacaa cgaggtttgg aattcctgat aagatttcca aaaaacgttg
     3361 catcacgtta tcgacacgtc cgcctaacaa accagaaacc aacccgtaag ctacaccaat
     3421 cgttacatct agtaatgcag caataaatgc gatcagcaac gagattctca ttcccataaa
     3481 taagcggctt aatacatcac gtcccaaacc gtctgttcct aaaaagaaat ttacgtttga
     3541 agggacgttt gcttgagcat atttatcaac aagttcacca ccaaccattg cttttccatt
     3601 caatccatcg atattgattc ccggaatacg aggaggcaaa ttgatataag ccacgttttg
     3661 ggcagttgga tcatgaggag aaacaaagat tgtgatcaca gagatcaaaa tgatgaccaa
     3721 tagtaaaacc attgaaataa cggcagcttt gtttttcttt aaacggcgcc atgaatcttg
     3781 gataaaattc aaagaaggtg cagtaatctg ttcacgttct tcttcggtat ttttttgtaa
     3841 tggaagaaat tcatccgcag ggatatctgc aactgtttca taacgctttg gtatcatttc
     3901 catttatcct cggctccctt ctgaaacacg aattcttgga tcaactagac cgtacaatag
     3961 atccacgatc aaaataacaa aaatcagcat aaatgagtaa aggatcgtta cagccatgat
     4021 cgtaggataa tcatttgttg tgattgactt aacgaattgt tccccgatac caggaatagc
     4081 aaagatattt tctaccacca gtgatccggt catcaatgct acagctaacg gtcctaataa
     4141 agtcatcaat ggaatcaaac tatttcgaag accatgttta aaggcgattt cccaacggct
     4201 caatcctttt gcttttgcta attccacata atcactatgc agtacttcca ccatttccgt
     4261 acggataaac cgagcagagt cagcaagcgg cgacatggcc aacgcaattg ttggtaagat
     4321 tgtgtacaca aagccatccc attttgcgat tggcaaaact tgtaatttga ttgcaaagat
     4381 atattgtaat aaaacagcaa atacaaagtt tggaatggaa cgacctaata tcgctacgag
     4441 tgtagaagat gtatctgccc atgtattttg cttcatggca gagatcgttc ctaaaatcgt
     4501 ccctaggact gttccaaaga taattgcctg taaccctaac tgcaaggatg gtccgattct
     4561 tccagctaac aaatgtgcga ctggctggtt tttaaattgg aaggaaattc caaaatctcc
     4621 ttgaagcaaa ttagaaagat aaatcccata ttgtacgata actggtttat ccaaaccgac
     4681 ttgtttattt agcatctcga ttgtttcagg actcaaacgt tcctgattcg tatatggcgt
     4741 gcccggcagc aattgcatca agaaaaaggt gattgttgca attaaccaaa gcgtgatgat
     4801 catgaaaaag acacgtttta atacatattt gatataacta ttcactaatg tttcccccta
     4861 gcttttgact tctgacagat gtttcgctac attattagat aatgaaccaa aaataaagga
     4921 gcaatcaaat gaaaaaaaat ctatccccta cgcagtagct tttctactga ttctggttat
     4981 tttaataaaa aatctcatca accattcctt tacactcatt caattatcta atgatctatt
     5041 cttatggtca ttgccgtttc tgattatcgg cggtttttta tgggtctttt cttcgggttt
     5101 cttcgatcat tttcagcgat cggtccatct tgcacgaaca aggaaccgaa agaaaaaacc
     5161 agaattttcc acgctctcgt ctgccagcta cggtatgtac agcttttggc tgatcattgc
     5221 cggaatacta attgctcttt ctgctatttt catgcttttt tctttgttag gataagtgaa
     5281 tatgagaacg aggttgcgac atttgccgca gcctcgtttt ttctcatgct cacggtatta
     5341 acgattattc ttcaacgtat gtccatttgt aatcccatga agcgcctgcc gcatggtgaa
     5401 cgataccttt gattttttca ttcactaggt gaccttctgc tttttggtac accggaacga
     5461 cacccatatc atcagagatg atcttgattg ctttttggaa atcatcccaa cgggcttgtt
     5521 catcgccagc gtctgttgat gaagctgctt taactgcttc gtcatattct gcattgctcc
     5581 agcgaccacg gttgtatgaa ttacctgtga caaacaaatc agtaaagctg cttgggtctg
     5641 catagtcagc agcccatccg cctagtactg cgtcaaaatc acccgaagtt gagcggtcaa
     5701 gacgtacaga gaatggaaca ggtgttggtt tgactgttac gccatccaag ttttcttgga
     5761 ttgagttttg aacatattcg attacttttt tcgatgaatc atcatcagaa gccatcaaat
     5821 caaattctaa agaatcaacg cccaattctt ttttcgcttt ttcccaatat tcttttgctt
     5881 tatctttgtc ataagaaact aattttccag cttcttttgc aaagtcttcg ttcgttgttg
     5941 gactttttac catatctgat gggatcaatc ctgtagagac aactgatccg tcacctagta
     6001 cttgttttac taaagcttca cgatcgatag aataagaaat agcttttctt aggttcacat
     6061 tgttgaatgg tgaattcttt tcacgttgat tgaattcgat atagcttgtc cgcgcttctt
     6121 taacagaagt ataagctgga tcgtttgcgt tttgctgtgc taactcacca gacaagatca
     6181 cgtcatctgc ttggccatct ttgaataagt tcaatgcagt agaagattct tttactacac
     6241 tgactttgat ttcagaaagt tttactgtat ctttgtccca gtaatgatca tttttcttat
     6301 aagtccattc tgtatctgtt cctggtccat caaaatcttc taatgtgaat ggtccattat
     6361 aaacagcttt atcacttgca gatgcatagg catccccatt ttcttccact acggattgtt
     6421 tttgtgggaa aaatgatggg aaagcaagca gatattggaa atatggtgtt tgtttcgtca
     6481 atttgatttc taattcatag tcaccaactg ctttgatccc tagttctgat ttatctttct
     6541 ttccagctgt aatgtcttct gcattttcaa ctggcgcata caggtaagca tattcagaag
     6601 ctgtagctgg gtctactgtt cgttgccagc cataaacgta atccgctgct gtaacaggat
     6661 caccatttga ccattttgcg tcttctcgta atttgatttt gtaagttaat ccgtcttcac
     6721 tgacttctgc caactcgcta gctcctgccg gctgtggttt gctgtcttca tcaagacggt
     6781 aaattccttc ataaacgtta ttcaatgctg taaaactgat tgtatctgtt gcaactgata
     6841 agtcagctgt aggcatttct tgaggcacga ctaagttaaa tacgcctcct ccatctgctg
     6901 aaccgctttt tgctgaacta ctgcttgtgg ctgaactgcc gccataacat ccagcaagta
     6961 caaggccgca taccgcaacc aaaccaaatg taaatttctt tttcatttta cgtacccctt
     7021 tattggaatt ttcagaataa ttaatatttt ctgaagttgt ttaagagttt aatgaataat
     7081 tattaaaaaa tcaacacttt tttttgtctt tttcataaaa taatgatttt ttcgtgaggt
     7141 ttcaaggaat ttttcagaaa aaatagcttt gtacaaggtt ttccatggtc ttccaaaagc
     7201 cattaaataa ttttcgttat attgtctatt attgccatac aaagataaaa aagaataaac
     7261 aaaaagaaac tgtgacaaaa gtcttgtcac agtcccttat ctga
//
LOCUS       DAZBSA010000118         7242 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000118 DAZBSA010000000
VERSION     DAZBSA010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.119"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..575
                     /locus_tag="ACHGMT_002483"
     CDS             <1..575
                     /locus_tag="ACHGMT_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFN6836196.1"
                     /translation="LLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGGEAVNWYNEP
                     KYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQIKHVTIPQLI
                     PLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTSTGDFGMTAA
                     AGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            587..1507
                     /locus_tag="ACHGMT_002484"
     CDS             587..1507
                     /locus_tag="ACHGMT_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN6836197.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1548..3005
                     /locus_tag="ACHGMT_002485"
     CDS             1548..3005
                     /locus_tag="ACHGMT_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN6836198.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3350..3964
                     /locus_tag="ACHGMT_002486"
     CDS             3350..3964
                     /locus_tag="ACHGMT_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFN6836199.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            3957..5681
                     /locus_tag="ACHGMT_002487"
     CDS             3957..5681
                     /locus_tag="ACHGMT_002487"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN6836200.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5678..7129
                     /locus_tag="ACHGMT_002488"
     CDS             5678..7129
                     /locus_tag="ACHGMT_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN6836201.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         2477 a         1206 c         1447 g         2112 t
ORIGIN      
        1 ccttactacc ttattttcta tcttgggtca ttatcagtta ctttgtttac gcttttttaa
       61 gtcctgacaa aggaatattc aatcagtgga tcactggaca tggaggagaa gctgtcaatt
      121 ggtataatga accgaaatat tggccgttta tcttggtgtt cattggtaca tggaaaggga
      181 ttggttataa cagcattatt tactttgcat ctgttatggg aatcgatccg acttattatg
      241 aagctgctat ggttgatgga gctagcaaat ggcagcagat caaacatgtg acgataccac
      301 aacttattcc actaatgacg attttaacga ttttagccgt aggaaacatt ttccgagctg
      361 attttggcct gttttataac attcctagga attccggtgc attatatgat gtgactcaag
      421 ttttggatac gtatatctac aatggcctga catccacagg agattttggt atgacggctg
      481 ctgcaggact ttaccaatca gttgtcggct ttgtcttgtt gatgatcaca aatacgatcg
      541 cccgacgttt tgatagcgat tctgcattat tctaaggagg aaaaaaatga aaaaaaagaa
      601 agtggagaga gtccaagtcc gctctttcaa taagacggct aattttttct ctaatataat
      661 gattgcactc tttgctattt cctgtatttt gccatttata tttgttatcg ccatctcttt
      721 taccgatgag tccatattgt cgactcacgg gtacagtttt tggccagaaa agttcagtat
      781 atttggttat acgtacttgt tcgaccagat gcaggataaa attttccaag cgcttttcgt
      841 cactgtactt gttactgttt taggtacact gatcaatagc acggctactt ctttatatgc
      901 ttatgcgatt tcacggtcaa actttccatt ccgtcgtttt tttaccgttt tttgtttaat
      961 tacgatgttg ttctcaccag ggatggtggc caattatctt gttatgacaa atcttcttca
     1021 gttaaaagat acgatttggg cattgatctt gccaatggct gtcagtccct tcaatattat
     1081 tgtgatgcgc acattcttta aacgttcggt ctctgattcg attattgaat cggcacggat
     1141 cgatggagct agcgaactgc gaatatttgt tcagatcgtt ttaccgttag ctgttccagg
     1201 tattgcgaca atcagtttat ttgccgcatt gggttactgg aatgactggt tcaatgcttt
     1261 actatacatc caagatgata agctagttcc actacagtat cttttgatga aaatccaaag
     1321 taatattcaa tatctgacac aaaattcagg agcaggaagc caattgacag gcggattagc
     1381 ctcagttcca ggagaatctg ccagaatggc gatcgtagtc atctcgacgt tgcctattgc
     1441 gatcagctat ccatttttcc agaagcattt tgtcaaaggt ctaacaatcg gcggagtaaa
     1501 agaataaaga aacaaaaata taaatcataa aattaggagg aagaaaaatg aagttatgga
     1561 agaaactagc ctttacaggt gtttcagcgt tgttgttggg aacattagca gcatgcggag
     1621 gttctggttc taaagaccag gcagaagcta gcaattcgga cactttgcag atgtatcaaa
     1681 tcggagataa accagataat ttcgatcaat tgatggaagt agcaaacaaa cgaatcgaaa
     1741 aagagatcgg tgtaaaagtc aatatcaatt acattggctg gggcgattac gaaaagaaaa
     1801 tgaacgtcat catttcttcc ggggaaaatt atgatatcgc ctttgcgaac aactatgttt
     1861 ccaatgccca aaaaggagcc tttgctgatt tgactgaatt agcgcctaag tacgctgaaa
     1921 aagcttataa tgatttagat gaagcatata ttaaaggaaa tctggtcaat ggaaaattat
     1981 atgctttccc agtcaatgga aatgtctttg ctcaacaagt acttactttt aacaaagctt
     2041 tactagataa atacgatctt tcaattgatg gaatccaatc ttacgctgat gcagaaaaag
     2101 tcttgaagga attccatgaa aaagaaccaa acacggcagc ttttgccatt ggacaaggct
     2161 tcaaagtcca agggaacttt gattatcctt tagggaatac gttaccattt gctattgatc
     2221 tggatggaga tgattcgaaa atcatcaacc aatacgataa tgaaacgtat atcggactgt
     2281 tacgtacaat gcatagctgg tacaaacaag gactcattcc aagtgacgct gcaacgagca
     2341 ataaggatta tccactagaa ggaaatactt ggctaatgcg tggagaaaca caaggaccgt
     2401 atgattatgg cgatacaatt ttgacaaatg cagcaaaaca agaacttgtc tcaaaagcaa
     2461 ttactgttcc actaaaatct acagctcaag cacaaatggc aaattttgtc gtatcaaata
     2521 cttcaaaaaa taaagaaaaa tcagtagaac ttctagggct gcttaacagt gatccagaat
     2581 tattgaatgg gcttgtttgg gggatcgagg gagaagcatg ggaaaaagtc gatggttcag
     2641 atgataaaat caaattgcta gatggttatc agccaaatac acatatgtct gcttggaata
     2701 caggaaacaa caagattcta tatacacaag agtctattac ggatgacatg attgccaaac
     2761 gtgatcaatc gattgctgat gcagaaactt caccgatttt aggattcagc ttcaacactg
     2821 acagtgtcaa aacagaatta agtaatattt ctaatgtcat gaatcaatat ttagatggat
     2881 tgaatacagg gactgttgat ccagatgaaa cattaccgaa attgaaagac gctttggaca
     2941 aggctggcta tgataaggta ttgaaagaaa tgcaaaaaca atatgatgag ttccgacaag
     3001 aataataatg aataaagaca aaggggttgt gacaaaagac gtgtcacaac ctcttgttcc
     3061 gaataaacgg tgttcaaaag ccagtttctc ggtattaagc taaatatatg taaaaatgtg
     3121 gaaagccata ttcacaaaat ttttcttact actcgaaact aagcggctat ttcacaaact
     3181 cttggtaaac ggtgttcaat cagctaaccc tcggtattcg ctcacaaaac gacaaaatat
     3241 gaggaactat ttttccgttt gtttctctaa taatcaggtc aaggtgctga ttgcccaacc
     3301 tttttttgta taatgagaaa gtataaactt aatgtttgga ggagaagata tgttagggaa
     3361 ggttttggaa gagttattta tccggatctg ggtcattata aaactaacct tgtatttctg
     3421 gatatataca tttgccggcg ggatcatctt tggattaggt gcggcatgga aaacagtcaa
     3481 tgaactattt tatttgtacg gattcgagta taaagaaatc acaataaaaa gaggctggaa
     3541 tatatataaa agaaattttt taagaggcaa tctgctattt agtttatttt tatctggtac
     3601 tgctttattg agttacaatt tatatttatc tgttcaaatc aaagggctca tttttttagc
     3661 tattgatttt attttacttt ttgccttgct ctgcctattt gctacttatc aatacagtat
     3721 gattttagat agtgaatata cgatctctgt tcctaatctg ctaaagctaa gttttatttc
     3781 ggttttttcc agcttttcgt cttttctaaa aataattatt ggtagcggta taattttagg
     3841 cgtgacttgg caattcaaag gattgatcct gtttggtgtc attggtcttt tgacggtttg
     3901 gaatggtacg atgacaacac attggcgtga agaattagat aaacagttgg aaagttatga
     3961 ataagtttcg gattctattt tcaaaaggaa ccttactaaa tcgtctgttg cggaaatacg
     4021 cggttatcat gatcggtgta acgatgactg ctaccgttat ttttagtatc catacttggg
     4081 aacaaaatca aaaacaggca gaaaacatga caagtgatgc cgttcaaagc acaagcagaa
     4141 tgctgaatga taaaacaacc ttgagtagga tcatcaaaaa ccagcttgta gggaattccg
     4201 aaaagatcga aaatgtgacg acctacttaa caaaacccat agatcagtat ctgatgtatg
     4261 tatacgaaca gcaaaacagt accgatgaat tagtttcttt cccaaatcaa atcaaagatc
     4321 tctacgcaaa ttatgaagaa ctaagtgcga tatatatcgt gttgaatcaa ctaccggagt
     4381 attatgaatc ttctagagaa aacaaaggtg gggaaataaa aaaggggaca ccaaaattac
     4441 tagacgcctt ttatatcaag ttgcctatca cacaaggagg agctgagtca ggaagtattt
     4501 ttattggttt tcaaaagaaa gaactagata tgattttaga aaatctaacg tcattcaatg
     4561 gactctcttt atacatgatt tctggaacga caaatcgact atacacattc catgataata
     4621 aaatagctaa agaaacgttt gaaagacaag aaatgatcat cacagagagt ctgaagaaaa
     4681 attcacaatt gccgattgac gatctggaaa aaaacaattt tgttcaacac caagaactgt
     4741 ctgatgatta tcgtatatta gctgtactag ataaaaaaag tgtaaaaatc gaaacgggaa
     4801 aaaatcttgc cccgctgatc attggtatca ttattttaga caatatactg cttatctttc
     4861 tttataaaac attcaaacga tattcacagc aagtatctat cgtcatgcag gccatggatc
     4921 aaacagcagc agggaattta gagacgcgga tcgacataac aaacacggaa tatgaactga
     4981 aggaactttc aatcggtatc aacgaaatgt tggatagcat caatcagttt gtagaagaca
     5041 tctacaaatt agaaatcaaa cagcaagacg cacatatgcg ggcgcttcaa gcacagatca
     5101 atccacactt tttatataat acattagaat atatccgaat gtatgcaatc agcgaaggaa
     5161 gtgaagaatt agcagatgtt gtgtatgcgt tttccgcttt gcttcgaaac aatacgaatc
     5221 aagagaaaac gatcacttta aaagaagaac tggacttttg cgaaaaatat gtctatttgt
     5281 atcaaatgcg ttatcctaat cgagtagcct atcattttat gatcgatcct gatttggaaa
     5341 agatcgaagt acctaaattt gtgatccagc cattagttga aaattatttt aaacacggta
     5401 ttgattttac tcgattcgat aatgcgctaa gtgtcaaagt cttgcaggaa ggcaaacggg
     5461 taaggatcat catcaaagat aatggaaaag ggatgactga aaaacggcta aaacagattg
     5521 aagaaaaact gtctcatcct aaagtggaac ttcacggttc tatcggtctg caaaacgtga
     5581 atgaacgttt acgtgccagc ttcggcagca gttattatat gtcgcttgag aataatgaga
     5641 caggtggact gaccgtttct attacattca aggagggatg agcgtgtatc aagtgttatt
     5701 ggtagatgat gaatacatga ttttgaatgg gttaaaaaag ataattgatt ggcaaagttt
     5761 aggctttcaa atagtagcaa cagcagagaa tgccaaagaa ggactagctg tacttgaaca
     5821 gcgacagatt gatttagtag taacggatgt gacaatgcca gaaataaatg gactggaatt
     5881 tattgaagcg gctcaaaaag aacgtcataa ttttgaattt atgattttat caggatacca
     5941 agaattcgat tatttgaaag gaggtatgca attaggcgcc gtgaattatt tgatgaaacc
     6001 cgtgaataag ttcgaattgg ttgaaagcct caagaaaatc aaaacacggt tagatcagca
     6061 aaatgaacaa aaaaatcaac aagaaatcta tcaagaactg ttattcagtc aatggttgaa
     6121 tgaagaattg gatgaaataa gtgaagaaga aataatagga caaataagtg agaagcagca
     6181 taggatagta ttgattcaac tggcaagaac tcatgatact cttatcaata gatggttgca
     6241 agaacggcaa caaatctttt actatcaacg aaattacgga gaccgtgtat tgatcacgtt
     6301 gttgttggag gcagatgaag ttgatggctt ctgttgtttt gttcaagaaa actttcctga
     6361 tcaggaatgg ctgatcagta tcggggaaga gacagattct ttggacaaaa tccctgaaac
     6421 attccaacaa gcgaaagaca atctacagtt gcatcaattt tatggagata aagacgagca
     6481 tgttttttac gcagtacaag cagaaacaaa agatcaaacc atcgattttt caagtttcag
     6541 acgcgtgctg caaaataaac aattggaagt cgctcaaaaa atgatcactg atttttttga
     6601 acagtttcag ttagcagcca tgatgccgga agatatacga tattccagtt ttttactgtt
     6661 tatggagatt caacgggaat tgattgatct ggaagatgag gaatatttac aaggaatcga
     6721 aaaaatccat caggcaaaaa cggtgcaaga acttcagcag ctacttctgt cattcatcaa
     6781 aaagcatcag agacagaaaa agtacagtga caatgtggaa aaagtaatcg taatcctgca
     6841 ccagcattat caagaaccac ttacactcaa agaagtgtca gaaaacctgc atttaaatgt
     6901 catgtatcta ggacagctgt tcaaaaaaga aacgaaaaaa agtttttcag cttatttgaa
     6961 ccatttacgt atggaaaaag cgaaacaact gcttttgcac agtaatcaaa acatcaatga
     7021 gatagcaagt gagattggct acaacaacac cacgtatttc tctaaattat tcaagaaaat
     7081 cgtcggcaga tcccctaaag agtaccgaga aaacagtgga gaatcataaa aaaaaaagcc
     7141 aatgtaatga aataacttat aaaaaattgt ctagaaatct aattttgggc tgttcttatt
     7201 acttgaggca aggcagcttt ttcatgacct ctcgataaac gg
//
LOCUS       DAZBSA010000119         7211 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000119 DAZBSA010000000
VERSION     DAZBSA010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.120"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..544
                     /locus_tag="ACHGMT_002489"
     CDS             <1..544
                     /locus_tag="ACHGMT_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836202.1"
                     /translation="QNAETIEREHHYFDLLVNTVVELAKWAKSNETYSQVLKRIMSQE
                     TSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRILKSLFDLKLAK
                     ATIMGIERDSYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLKCRGLDFIGTLG
                     WTETYYEKIYKEIIEQYFPK"
     gene            727..3402
                     /locus_tag="ACHGMT_002490"
     CDS             727..3402
                     /locus_tag="ACHGMT_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFN6836203.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            3584..3823
                     /locus_tag="ACHGMT_002491"
     CDS             3584..3823
                     /locus_tag="ACHGMT_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836204.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3839..5212
                     /locus_tag="ACHGMT_002492"
     CDS             3839..5212
                     /locus_tag="ACHGMT_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN6836205.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            5216..6850
                     /locus_tag="ACHGMT_002493"
     CDS             5216..6850
                     /locus_tag="ACHGMT_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN6836206.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTSLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            7013..>7211
                     /locus_tag="ACHGMT_002494"
     CDS             7013..>7211
                     /locus_tag="ACHGMT_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836207.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2568 a         1130 c         1436 g         2077 t
ORIGIN      
        1 tcaaaatgct gagactattg aacgagaaca tcattatttc gatttattag tgaatacagt
       61 ggtagaatta gcgaaatggg caaagtcaaa cgaaacttat agtcaagtac ttaaaagaat
      121 tatgagtcaa gaaacttcat ttatctatga gttaagcaag tcaaatttga aatatactta
      181 taatgaagat gaacttgaaa ttttagataa tccagaaaat tcacaatgga ttaaagagtt
      241 gaagagagaa gactttattg agacactaag aattctaaaa tcgttatttg atttgaaatt
      301 agccaaagct accatcatgg gaattgaaag agattcatat tattgggagg aaattggact
      361 atatgctccc cagattgaag gtgaagataa agagaaaagg atagacaaca ttattaacta
      421 tattaatagt ttaagtgaac ctttaaaatg tagaggactt gattttatag gaacattagg
      481 atggacagaa acatactatg agaaaatata taaagaaata attgaacaat attttcctaa
      541 ataatttaat aaggattgat ttatcgtaaa agaaaaaagt tgtgatttcc aaaatcacaa
      601 cttttttctt ttgtcaagat tttgagtggg atatattttt gtagaactga agatcatgaa
      661 attatgagag gttggaggta atgtagtcat acaaataaaa attttttaga aagtgagtgg
      721 taattaatga aaatgaatca cacttcaatt cagaagcatt tacaactgtt aaatacactg
      781 gggatttctt atgttaattt gagaactttt tctacattaa acaagaaaac cgtttctcat
      841 attttttcta ctgcagattc tcaagcaatt attaactatt tagaaaaaca gaaattgtct
      901 gaaggtacag taaacataac ttataatcct attaaacaag aggtgattac acaaaaaaag
      961 cattctgtga gagacaatga tattgaaaaa attcggtttg tatttattga tattgaccct
     1021 aaaaggaaag agggaagtgc aacagatagc gaaaagaaaa aggcagaaaa tgttatggaa
     1081 caagtagaac gttatttgaa agaaaagaga atagaatcat ttgtaaaagt tgatagtggg
     1141 aatggctacc atatttttct tcccataaat gaacaaccaa ataatcacga gacgattctt
     1201 actattcaga attttcttca attacttcac cgaagatttg gagtagaaga tgaagttgat
     1261 attgatactt ctgtttataa tccttcaaga ttatgcaaat tagttggtac accagcggtc
     1321 aaaggaaagt ccacaaaaga gcgtccgcat cgtttatcta gtttcctttc tattcccgat
     1381 aacatagata gaagtaattg ttttaaaatt cttgaacaga tagttgaaga aaattccatg
     1441 atgtcaacct ctagagtaaa aagccatcca tcttttagag aagtgttacc ttttattcaa
     1501 gcagactata aacaatggtt agctcactat cctgaattaa cttataccat aaaagagggc
     1561 gatcataaag gagttacctt acttatattt gacgaatgtc cattaagaaa acacacgaat
     1621 aattctaatg gttcttgttt gtctgttacg gctgataata agattcgatt tcattgtctg
     1681 catgcttcgg ataaaaataa agatattcaa gactttgtaa aaaaatatcc gttgccaaaa
     1741 cctgtaaatc cttctgtaga atcattagaa tccaatcaag aatattattt tgaccagttt
     1801 aaactgaatc aagaaggagt atttttggtg aaagaaggtc aagaagaaaa agttgcttgt
     1861 ccgatgttta ttaagcaaat acgcaaaaat atcgaacaaa aagtcattac acttgttctt
     1921 gcctattatg tcgatcattg ttgggatgaa atagaaatag aaggtggatg tttacaaact
     1981 aataatttta aacaattagc aaaacatggc ttaatcatta aagctagaag agaagctgaa
     2041 gtcgttgact ttttacagat tcaacgggag aatgctccaa tcttttttga acatgaatct
     2101 cttggctgga aaaaagaaaa tgaagcaatg atttttcagt tacaagattc ttttggacgt
     2161 gaaattcctt ctgtattaac taaaaactca tgctaccaat taacaactaa tggtacagaa
     2221 gcggaatttg acgcattggt tcataagtat gttttaggaa cgaacttaga acttgcttgg
     2281 tcgattggta ttgcttctat tgtgttaggc tatttaaacg ttacgaaggc taatactttt
     2341 ttgagtttac taattaacct acagggcaag agcacaacgg gtaaaaccac tacgttacac
     2401 tttattgcct ctttatttgg aaatccaact acggttctta gaaatatgaa tgctaccaat
     2461 aaagccatta taaagctagc atcaaacaat cagggaggtg ttccactaat actagatgag
     2521 ttaggagcag caactcaaaa taactttagc tcgttgatct atcaattagc ttcaggagaa
     2581 gaaaggctaa gattagattc taattgtcag cttatgccac aagagacttt ttctgttttg
     2641 acccttacct cttctgaaga gcgtttggaa aattatttca atgagaaatt aactggtttg
     2701 aaagttcgat atatcgaatt ttctgatatc aagtttacaa aggatgcgaa tcacgcagag
     2761 aaagttaaaa gtctttcttc taatactttt ggttggggta taaagttatt tcttttgaaa
     2821 ctctttgaac aagggattac aaaaattgaa gcaacttttg tcgaagctca aaaaaaacta
     2881 tgggctgttt tacctgaaca ttctttgaag caacgcattg gtaataatct agctttgatt
     2941 tatagtggag ccctcctaat tcaagatata ctgggctatc ctttacgatt agagcatata
     3001 cagacggtgc ttattaattc gtacgaacag ttaattgtag atactagttt aggaagtgag
     3061 caaacggtat ttgaccgagt gaaaacattg ttggtgatga atagccataa atttttaaca
     3121 aacagtgaca acaaacatac gcccagtacg atttggggaa aagtatcaat tcaaaaaaat
     3181 ggggatatta aggtaaatat ttttcctatc ccgtttgagg acttgttaag gcgagaattt
     3241 caagtaaagg atctaaccta tctttttagg caattgcttg attcgggtaa tatgtgtgca
     3301 gaaaaaggga gaagaacgaa acgtattcac atgaatcgtc aagttcttcc aacatacgag
     3361 attctattac cttcttcact caaatcgtat tttcgattgt aatttagaat aaaccaaagt
     3421 atgaaagtga ggcaccatgt tatggaaata tttcttattg atttagcaaa acaactgatt
     3481 acttatctgt atgatagcga gcaaacccaa acggaagtaa atgaagcttg atctatgctt
     3541 catttacttc attcaatcaa acatgaaaga aaggaaaatt ctaatgacta aaaataaaat
     3601 gacactaaaa gcagaggttc ttctgtatat tcaagaacat ttttcaaatc aagccttttt
     3661 tacaaaacca atttacttgg atttcgaaat aagaggggta tctgctggat ctatcggggg
     3721 tactcttcaa gcgttgaaga acgaaggata tttagaaaat cactttgtgc aacgttcgtt
     3781 caatagaaga gtcgtgaaaa aatggtattt agttcattca tagagagggg aatcaagcat
     3841 gcgtgtggct tgttatgatc gaacgtctgt ttatgaagaa gcgaaaatta gtaatgacgc
     3901 tcaatatgat gcagtgaaag agtatgtttt agagaaatat gaacaaattc cccaacgatt
     3961 ttcagacaaa ggagaatcag gggaaaaagc ggagcgtcca gcttttcgac agcttagagc
     4021 ttgggtttgt gcgtcagaag aagagggggt gatctatgtt tggcgttatg atcgattggc
     4081 acgtcaagcc aaaatagcct tagaatttat tgagttgtgc caacaacatc aggttcaagt
     4141 gtattcgatt tctgaacctc ttcctgcttc tttctcacac gcgtcaacga ataaactatt
     4201 tgtgcaatta ttgttcactt ttgcagaaca acaacgaaac gtcattattg agaatgttcg
     4261 tgccggactt tcctataaaa agcaacaacg gcaatatttg tcttctgcgg taccatatgg
     4321 gtatcgttta gtgaatggga aaatccaaca agaagtacac gaagcaagga ttgttcgcta
     4381 tatttttcaa ctttatttga ccaaaaaata tggctacaaa aagctttgtc aacgattgac
     4441 gcaacaaaag cttttcttcc gcgaacgtcc ttttcagccc tatcatattt atagtatcct
     4501 aaaaaatcca ttgtactatg gcgaaatcaa aggaggatct ttggggaagt atctaggaac
     4561 ctttgaaccg attctctcga aagcaacctt tttacaagcc caagagattc gccaatcaag
     4621 gtgtacagct aaaaaagata cgtatctgta cttattacgt caaaaaattc ggtgtccctt
     4681 ctgtggtcgt catctgagtt cgaagtatca atggaataca aagaagacga agacgcttca
     4741 ttattatcat tgtaccgacc gaagctgcca aggaattttt cttcgggcag aaagggttga
     4801 gtcagaagta ttggaaaaga tacaggcttt ttttcaatac gaaggcgtct atcaagcctt
     4861 gattgcccaa ttaacgcaac aattagccga aaagcgaaaa gatgaaaaac gcaaagtaat
     4921 ccaacaaaag aaacaagaag cacaatggat taaggcattt gaagagggca attgttcact
     4981 ggaagaactc aagcaacaac tgcaagctct tcaaaagccg atgtctactt cttcgtttct
     5041 ttcactccaa cagtgtgaag caaaagtgaa agaatggctt caactaaaag acctctctag
     5101 tcgacaatta ttatgtgact atgtttgtcg gattgaggta acaaaggata aaacagtttg
     5161 tgggatttac ttccgacaac tgacacaaaa tattttagaa aggaacgaat gatctatgga
     5221 aatgatacaa accaaacgag tcgcattgta tattcgcgtg agtacacaag aacaagcttt
     5281 agaaggacat agtattcaag cacaagaaca aaatggacga cagtttgccg aacggatggg
     5341 ctatgaagtg gtagaagttt atgccgatga aggaatcagt gggaaatcca caaaaaatcg
     5401 gttcgcctat caacgaatga tgaacgatgc tcggaaccat cgttttgatc tactagtgat
     5461 ttggaaattg acccgtctcg gacgcaatat gttagatgtc ttacaaacag tcgaggaatt
     5521 attgcgatat gacgtaggat tacattcgat ctcggaacaa tttgacgtga ccacttcgac
     5581 aggtaaattg atgttacaac tccttgggag tttcggtgaa tttgaacgca accaaattgc
     5641 ggagaatgtt caaatgtcaa tgcaaagctt agtgaaagat aaaaagcgtt atgcaggagg
     5701 ccgacgttta ggctatgtca gtggtattga cacgaatggt caaaaacaat tggtcattga
     5761 accagaagag gcgaaaattg ttcgtctgat ctatgcaaaa tatttaggcg gagcagggta
     5821 tcgagcgatt gccaattttt tgaatcgtca aggatatcaa acagtcaaaa agaatgcttt
     5881 ttctaccacg gcagtcaaag atatcttacg caataaaatt tatgcagggt atttagaata
     5941 tgcgcgctac atcaattggg aaaccaaacg acggaagggg aagaatccga gaccgatttt
     6001 agtgaaagga acccatgaac cgattatttc ggaagaagat tatcaaaaag tacaagaacg
     6061 ccttgcttta gaaggaaaac aacccaaatg gaaccataca ggtgaaaatg tattgacagg
     6121 cttgttacgt tgtccagaat gtggcgcacc gatggcagcg agtaatgtca caaataccct
     6181 aaaggatggc acaaagaaac ggattcgata ttattcttgt tcagtctttc ggaataaagg
     6241 agcaagtgtg tgtcatgcca attcgattcg tgccgaacaa gcggaaacat ttgtcgccga
     6301 ccgattaaag gagatcgttc agctaccaga agttcttccc cgattggtgg cagcgttaaa
     6361 tgaagaaata gtgcgacaaa gccaaccatt ggaacaagaa ctcgtggtgt tactggaacg
     6421 aaaggaagaa ttgaagacta aaattgaaaa atgggaagct gctttggaag atagtcctga
     6481 attgtttccg atgttaaaag accgcttaga cgaattgact gaaaaacgac gacagttgca
     6541 cattcgagaa aatgaaatct tagggatctt tcaacaagga gagccgattc aagtcaaaga
     6601 tgtccaacgg atattgacta gtttggatcg ttttcttgcc cagagtgaga agaagcaaat
     6661 caaagccttg tatcgtacct ttatagagaa aataaccttt gatcctaacc ataaggagat
     6721 gttggaaatt acgatgaaat tcacgcatgc cgtggtacaa caattgaatg agcaatatca
     6781 aatagccgta tccaaaacaa aggatacggc tatttttgtg ttaaaaacac cctttacgtt
     6841 aacgatctag tctaggttat atcaaacaac gttagagggg cgctaacgct tctctagcga
     6901 tttgaaatcg ttagagaagt agttagggag gtccttatca taaataggga tatttattaa
     6961 tgaatatttg aaatataatt gtatataaga attaaaaaga gtggggatag atatgaaaaa
     7021 gatgggtaag tgtattttat gtgaaaaata cactgaactt actaaagaac atgtaccacc
     7081 taaaaaatca gggaatactg gaggtaaaaa gaccagaact ggtaatttag atgatttcct
     7141 taagagcgat tttacaaaag gtgattttcc gaaggggatt aaaaggaaac ctcaaggaaa
     7201 tgtttattat a
//
LOCUS       DAZBSA010000120         7166 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000120 DAZBSA010000000
VERSION     DAZBSA010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.121"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(68..1516)
                     /gene="cls"
                     /locus_tag="ACHGMT_002495"
     CDS             complement(68..1516)
                     /gene="cls"
                     /locus_tag="ACHGMT_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFN6836208.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(1668..2552)
                     /gene="ftsX"
                     /locus_tag="ACHGMT_002496"
     CDS             complement(1668..2552)
                     /gene="ftsX"
                     /locus_tag="ACHGMT_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFN6836209.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            complement(2545..3231)
                     /gene="ftsE"
                     /locus_tag="ACHGMT_002497"
     CDS             complement(2545..3231)
                     /gene="ftsE"
                     /locus_tag="ACHGMT_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFN6836210.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(3336..4437)
                     /gene="prfB"
                     /locus_tag="ACHGMT_002498"
     CDS             complement(join(3336..4364,4366..4437))
                     /gene="prfB"
                     /locus_tag="ACHGMT_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFN6836211.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(4563..7097)
                     /gene="secA"
                     /locus_tag="ACHGMT_002499"
     CDS             complement(4563..7097)
                     /gene="secA"
                     /locus_tag="ACHGMT_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFN6836212.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
BASE COUNT         1910 a         1597 c         1252 g         2407 t
ORIGIN      
        1 aaatggcccc gttagaaaag gacacgagat ctccatctgt tgatggttct catgtccttt
       61 ttatgtttta aaggagcggc gaaatcaatc ttgctaatcc ttctttcatc ttgatgatta
      121 ttcctcggtt ttcatacatt tcttttgtga gatgcgtaga aattttcgag tcttcaaaaa
      181 aagcttttct tacacgtgaa gccattcgtt catcataaac cagtgtattg acctcaaagt
      241 ccaagcgaaa tgaacgaaca tcgatatttg ctgagccaac tgaaacaatg ccaccatcga
      301 taatcatcgt ctttgcatga ataaatccat tctcatatgt ttcgatcctt gctccatact
      361 cgattagctc tgcagcgaaa gaatacgttg cccagtaaac gagaagatga tctggtttgt
      421 taggtatttg gatatgaact tttacgcctg aaagaagagc caacttcagt gcttcatgaa
      481 tcgatccatc cggtatgtaa taaggcgttt ggatcaatat ctcccgtttg gctaacgaaa
      541 tcattttcaa ataagtcatt ttgatttgtt catgttcggt atctggtccg cttgtcacga
      601 tttgaacagc tatatcacca gtagagtgaa tggacggaaa aaatttggga gaatagccta
      661 cttcttttgt atgctgagaa ttccagtcca tcaaaaaacg gttttgcaag gtatacaccg
      721 cttctccgta aatacgcaaa tgattgtctc gccaataacc aaatttcttt accaatccta
      781 gatattcatt tccaacattg aacccacctg tatatccgat tttcccatca ataactacga
      841 ttttacgatg attgcgataa ttgatccgag gattaatata cggaacaaat agaggaaaga
      901 aaaatgcgac ttctccgcct gcttttttca aattctgaaa aaagctggaa gaaacttgtg
      961 tggaacccca tgcatcaaga aggactctga cttttacccc tcgtcttgcc gcctctgtca
     1021 atgcatctcg aacttcttca cctaatgcat cactgcgata aatataatac tggaagtgga
     1081 tatggtcttc tgcctgcttg atatcttcaa tcagcgcatc gaatttttca cgaccatctg
     1141 taaataaagt gatttcattt ttagtcgtat agagcgtact ttcaaatacc gtgagcatat
     1201 agatcaattg tttcacatct acttgtccag taggcggatg aggataaagc cctcgcatca
     1261 atgcctgttt gtcttcttca atttccaggt tcttaccgat tttcccttgg atcttcaaat
     1321 caaagatttt ctctttggaa atccctcttc ctaaaaaaat atataaaata aatcctacaa
     1381 ctggaatgaa catcaaaacg agcacccatg cccatgtttg agcagtttcc tttctttctc
     1441 gaaaaacgat gataaaggaa agcaaaatat tcataatcaa aaggatcgta aaaaaattgt
     1501 caacgataat tttcacggct tcacctcttc ttctaccaac ttaagaatac ccaagttgtt
     1561 ttttaaagta aagggaaaaa agccgcttcc ttctattctc ctagccaata aaaaaagaaa
     1621 gctggaacga aactgtccaa ctttctcatt ttatttatat tgtggtatta gaatttcaag
     1681 aaacgtctca tagaaatgat cgagccaagt gaaccaatga tcacaccaat gccgaccatt
     1741 aaaagattga ttttccaaac gatattttgt ggatgaatca atgaataatg ggaacgtaat
     1801 aatgatggat tgatcaattg gaaaaaccat gcataaccaa atgtgatgat caaaatcgga
     1861 agaatcgcac cgatcagacc gatccagcca ccttcaagga aaaacggcca gcgaatatat
     1921 ccattttttg caccgactaa acgcatgatt tgaatttcac gctgtctaga caaaatcgtg
     1981 atccgaatcg tgttcgaaat gagaaacatc gctacgaaca gcagcaatgc cgctgcaact
     2041 aatccccatg tccgaactgc accagcgatt tggaaaatcc gatctgagga agctccaccg
     2101 taatcagaac gagaaacatt cttcaaatct gctgcttctt ctgctacttg ttttgtataa
     2161 gaaggatcag tagcacttac tacatacaca tcatataacg ggttattgtc accttcaaac
     2221 aatttccagg aatcacccat ttcgttttgg atttttttca attcctgttc tttgcttgaa
     2281 aattcaactt ttttaacatg atctaagtca ttcaattctt tttctagctt tttcatctct
     2341 tcgtcttttg taccgatatc tacgaataca gagacatcaa cgtttccttc tatatcttga
     2401 gctaatttag ttgcgttcat gataactgcc atgaatacac cgactaaagt caatgtgatc
     2461 gttaccgcac tgactgaagc aactgtcatc cagccgttac gtttcaaact cttgatactt
     2521 tctaataaat ggcggaaaaa ggttctaatc atcgtatcca tattcccctt ccgcttgatc
     2581 tcgaataatg cggccgtttt cgatagcaat tactcggtgc cgaatcgtat ttacgatcgt
     2641 actgttatgt gtagccatca cgacagtagt tccttgggca ttgatccgat ctaataattt
     2701 catgatttcc catgagtttt ctggatccag atttcctgtc ggttcatccg cgataagtac
     2761 tttaggcgta ttcactattg cccgagcaat agagacacgt tgctgttccc caccggataa
     2821 ttcgctagga aagactcgaa ctttgtgttt caaaccaaca aggtccagca cttccattac
     2881 tcgttttttg atatcccgag gttttctgcc gataacttgc atagcgtacg caacattttc
     2941 atatactgtt tttcttggta ataatttata gtcttggaac accacgccga tttctctgcg
     3001 tagataagga acttcgcggt ttttgattgc tattagatca tgacctgcga cgttcaaagt
     3061 tccttttgaa gctttttctt ctcgatacat caatttgatg aatgtagatt taccggctcc
     3121 agacggacca accacataga caaactctcc ttgttcgatg acaattgaga gattgcggat
     3181 agcggtcgta ccatttgagt acttcttcat tacatccttc atttcaatca tggcgttctc
     3241 tccatatctt tttcagtctt tagctattat agcatggcaa tgtttcaaca tgcttgcaaa
     3301 atcttacaat tttatttcaa tttaacaatg ggttcttatt gtagatgcat ttttaaatag
     3361 ccgtcaataa ataaatcaag gtcaccgtcc ataactgctt ggacatttcc tgtctctacg
     3421 tttgtccgat ggtcttttac catagaatat ggatggaaaa cataagaacg gatctgtgaa
     3481 ccccagccga tttctttttg ttctcctcga agtgcagctg cttcttgttc tttcttttcc
     3541 acttctaatt gataaagctt agcttttaac atccccatcg cttgctcacg gtttttcaat
     3601 tgtgaacgtt gtgcctgact agctactact gttcctgtag gcaagtgagt gatccgtaca
     3661 gcagattctg ttttattgat gtgctgacca cctgcaccac ttgctcggta cgtatcgatt
     3721 ttcaaatcat ctggattgat ttctatatcg atcgtttcat caagctctgg catcacatct
     3781 accgaacaaa aagaagtatg acgtcgtttc gctgagtcaa aaggcgagat acgtacaagg
     3841 cgatgaacac ctttttctga tttcagataa ccaaatgcgt tgtgtccttt gatcagtagg
     3901 gtcacactct tgattcctgc ttcatcacct gcttgataat ctagtgtctc tacttgatac
     3961 ccatgctgct ctgcccagcg tgtatacata cgtaaaagca tactgcccca atcttgggac
     4021 tccgttccgc cagcccctgg atgcaattca acgatggcgt tgtttcgatc ataaggacca
     4081 tctaacaaca tagataattc atatgtcttc attttttcat ctagcgcatt gatacgtgtt
     4141 tctgcttctt cttgcatggc tgcatcaggt tcttcctgca acatctccac catcaattcg
     4201 atttcttcca gttcttcagc aagagaatgg aattgatcat aagtttcttt atttgcattg
     4261 ttttcactga tcactttttg agcagcttcg ctatcatccc aaaaaccagg ttcagccatt
     4321 tttgcttctg cttccgcaat gtcttcttcc agctggtcta agtcaaagag acctcctgaa
     4381 gcttgtgacc ttttcctgca tttctgtcag gagatttcga atttcggata tttccatgtt
     4441 ttttccttct ttcactatac ttttttcatt atatctagtc taacagaatc agcaaataac
     4501 agaaagaagg tgagacgaat tcttaaaaaa agaatttgtc tcaccaatcc tgctgtcaga
     4561 aattagtttg cattttttcc atgacagttc ttatattttt tcccgcttcc acaaggacaa
     4621 ggatcatttc ttccgacctt gttcacatgg attggctgtt ttttcgcgct agtattacta
     4681 ttttctttgg catctccgtc ttctgagtga gaagctgttc cttgagaaac ctgttcacgc
     4741 tgtacgtttt gacggatctc agctttcata aagattcgag tgacttcata ctcgatggcg
     4801 cctaccatat cttcaaacat cttatagcct tctgtttgat attcgactaa tggattgttc
     4861 tgtccataag cacgtaagcc gattgactga cgtaattgat ccattgcatc aatatggtct
     4921 gtccatttag agtccacaac acgcaagatc acgacttttt caaattccaa cagttgctct
     4981 ggaccattta gttgagccgc tttggtatta aatacttctt gtgctcgttc gtttagatac
     5041 tctttgatct cctgagctgt ttttccttcg atatctgcaa gagtaatact atcttcatgg
     5101 acaagagtac tgccggcaaa atcgatgata ctttccaaat tccaattttc tttctctaat
     5161 tgtgtatgcg catctactac acgactgatt gtccgtttga ccatgttcat tagtggcttg
     5221 gataagcttt tttcttccat gattacttct tgtcgctgtt tatagattac ttcacgttgt
     5281 tcacgcatca catcatcgta ttgtaagacg tttttacgtg tgtcatagtt atttccttct
     5341 acacgttttt gtgcagattc tacttgtttc gtcagcatct tgctttggat aacagcatct
     5401 tcctcaccga tcttcatccg atctaagaaa gatttgattc gttctgaacc gaatcgtttc
     5461 atcaaatcat cttccaatga caagtagaac tgtgacatac cagggtctcc ttgacgtcct
     5521 gcacgtcctc gaagctggtt atcgatacga cgagattcat gtcgttctgt cccgataact
     5581 gctagaccac caagttctct taccccaagt cctaacttga tatctgttcc ccgaccagcc
     5641 atgttggtag caatcgtcac agcacctttt tgaccagcat tcatgatgat ctctgcttct
     5701 ttaaagtgat ttttcgcatt cagcacttca tgaggaatac gttcttgatt cagcatatct
     5761 gataataact cagatgtttc aaccgcaaca gttccgacta acacaggttg acctttgcgg
     5821 tagcgatctt tgatatcttg cacgacagcg tggaacttgc tttgtaatgt tgggtaaagc
     5881 aaatctggac gatcatcacg gataaccgga cgatttgtcg gaatctggta tacttggatg
     5941 ttataaattt ctctgaattc ttcttcttct gttttagctg taccagtcat tcccgcaagt
     6001 ttcttgtaca tacggaaata gttctggaaa gtgatcgtcg ccatggtttt cgtttcgtct
     6061 tcgatctcta cgccttcttt agcttcgatt gcttggtgaa gtccatctga ataacggcgt
     6121 ccatccatga tccgaccagt gaactggtca acaatcagta cttttccgtc ttggacaaca
     6181 taatcgatat ctaaaagcat gatatagttg gctctcaatg cttgatccaa gtgatgggtc
     6241 aatgcagtat tttcaatgtc gtataagtta tccaaaccga atgtttcttc tgctttttcg
     6301 atccctgctt cggttaaact gattgttttt gattgtacat cgattttata atcttcttct
     6361 tctttcaaac gtttcacgaa gttgtctgct cttgtatata aagctgtgga tttttctgct
     6421 tgccctgaaa tgatcagcgg cgttctcgct tcatcaatca agattgagtc aacttcatcg
     6481 acaatggcat aattcagcgg acgttgtacc atttgatggc ggtaaacgac catattgtct
     6541 ctcaagtagt caaatcctaa ttcattattc gtactataag tgatatcaca attataagcc
     6601 tcgcgttttt catccgaaga ttttgagttg atattcaacc ccactgttaa tcctaagaaa
     6661 ttataaagtt cgcccatttc cgtcgagtca cgggttgcta aatattcatt gacggtaact
     6721 acgtgtaccc cttcgccagt cagagcattt agatatactg gcatcgttgc ggtcaaggtt
     6781 ttaccttcac cagtacgcat ttcagggata tttccatcgt gaagcacgat tccacccatc
     6841 aattgtacgt ggtatggata taaacctaat acacgttttg ctgcttctcg gacgactgca
     6901 aaagcttccg gaagtaattg atctaatgtt tctccttttt ggtatctgcc cttgaattca
     6961 gcagtttttg cctgtaattg ttcatcagtt aatgctgcca tttggtcagc gtatgcttca
     7021 actttttttg caatgctatc taaacgtttt agttctttct tatcgttttc gataagtgaa
     7081 cgaatcaaat tggccatgcg ttgtgtaatc tccttttcct tctgtgtatt atccagacta
     7141 tgtagacttt acttttcatt ttatca
//
LOCUS       DAZBSA010000121         7142 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000121 DAZBSA010000000
VERSION     DAZBSA010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.122"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..280
                     /locus_tag="ACHGMT_002500"
     CDS             <1..280
                     /locus_tag="ACHGMT_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836213.1"
                     /translation="NEVIKRKLSHGYFVSYELYEESKINLKKKPKYYKNLKTGAIITE
                     KEFKNLVTEEESRFYNLSGNICPGFMDQIIENESIESIENYVPVDLPK"
     gene            complement(453..656)
                     /locus_tag="ACHGMT_002501"
                     /pseudo
     CDS             complement(453..656)
                     /locus_tag="ACHGMT_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            622..819
                     /locus_tag="ACHGMT_002502"
     CDS             622..819
                     /locus_tag="ACHGMT_002502"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836214.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(862..1437)
                     /locus_tag="ACHGMT_002503"
     CDS             complement(862..1437)
                     /locus_tag="ACHGMT_002503"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFN6836215.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1592..2118)
                     /locus_tag="ACHGMT_002504"
                     /pseudo
     CDS             complement(1592..2118)
                     /locus_tag="ACHGMT_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2575..4086
                     /gene="gadC"
                     /locus_tag="ACHGMT_002505"
     CDS             2575..4086
                     /gene="gadC"
                     /locus_tag="ACHGMT_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HFN6836216.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            4099..5499
                     /locus_tag="ACHGMT_002506"
     CDS             4099..5499
                     /locus_tag="ACHGMT_002506"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFN6836217.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(5664..7099)
                     /locus_tag="ACHGMT_002507"
                     /pseudo
     CDS             complement(5664..7099)
                     /locus_tag="ACHGMT_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT         2243 a         1206 c         1275 g         2418 t
ORIGIN      
        1 caatgaagtt attaagcgaa agttgtctca tggttatttt gtcagctacg aactatatga
       61 agaaagtaaa ataaatctga aaaaaaaacc aaaatactat aaaaatttga aaactggagc
      121 tattataact gaaaaagaat ttaaaaattt agtgactgaa gaagaaagca ggttttataa
      181 tttgtctggt aatatttgcc ctggatttat ggatcagatt attgaaaatg agtcaattga
      241 atcaattgaa aactatgttc ctgttgactt accaaaatag aaaaaataat cctgttttta
      301 cagtgaatta ttttaatttt gattttcaat aaaaatatat tacaataata atcgtaatag
      361 ctaatgaaac aatttctcaa tctttttacc actccaaaca gggagcgtca ataattttgt
      421 gtaaataact cgtccttctg caaaataatg ggttactcag taaacattga agctaatgta
      481 tccgtcactt gttggaagcc tttatggctt ctatttaaga atttttgatt gtatgtatca
      541 aagatggata ctaggaaacg ctctagtgat tcttcatttt gaaactgctc ttttctgcgg
      601 ctgtatttct tgatttgttt attgaaagat tcgattgtag aaccagaagg aatggagaac
      661 acttttgaac tttacaacaa aactactgtt gataaattaa ttccaatttt agttccaact
      721 gatttaaatg gtcatgatgt tttacttcgt tttaacggat ttttaggtga ggattataga
      781 tacctaaaaa tttctgatcc tcaaccttta acagaataat aaaaaacgtg taatcaatta
      841 tttgattaca cgttttttat ttcaatcttc gttgtagtag tggttgcgac ataccgaaag
      901 gtaatcttca ttacctccta ctacaatctg ttcacctgaa taaactggaa taccatcaac
      961 aatacgagca ttcattacag cctttttatt gcaaaaatgg caaatagttt taatttcctc
     1021 aattttatca gcaaatgtca ataatgcttc ggatcccttg aagagattat tttgaaaatc
     1081 attcttcaat ccatagcaaa ttactggtac ttcctcattc attacgataa gtgatagatc
     1141 aataatattc tctctactta aaaattgaga ctcatcgacc aaaatacaat caacttttcc
     1201 gtactgatca ataatctcca ctaattctcc gtacacatcc atttcagaag taattgcaat
     1261 agctggtttc gaaacaccta tacgactctc aacttttcca aatcctgatc gagtatctat
     1321 tgcgcttgtc attatgataa ctctcttccc ttgttcttca taattatgag ccactttcag
     1381 tagctcaata ctctttccac tattcattgt tccatatctg taaaataatt gtgccattaa
     1441 atttattccc ttcttttaca attcaattat tccttaattc catctgcttt tatgcagctg
     1501 ctcgtttatt ccttgatatg tctataacat ctactatccc taaaaccaat ttataagcca
     1561 ttagagatgg tattcctgaa catctctcat attgtttttt gagtggtata tcatctgaaa
     1621 aactgaagga atcatgaaaa tagcgacata cttatcattt agagttctaa ctttaaatgc
     1681 atttacttct tcagtcagca ctttagtcat gttggacatg gtttggggcg tgtagtgatg
     1741 cccatacatt ttctcaatca agtctgcgat ttccgacatg gtaatacctt ttcggaagag
     1801 gtgaatgacg gtctcctcta atgtgtcatt cgtccgtcta taagcaggaa cagtctgttg
     1861 cttgaactcg ccattgcggt cgcgcggaat ctggagatga agttccccat actcggtctt
     1921 gaccgtacgg tcataggagc cgttacgaga attacccgtg ttaaaaccaa tgcgatcgta
     1981 cttttcgtaa tcgaggaaag ccgttaactc cgttttgaga agcgtattga cagctttttc
     2041 caaatggaca cggaataatc cattcaaatc gcctttgttc gctagagtct ttaggatttc
     2101 tgtagtaaaa tcgttcatat ggaagtcctc ttttctgtga atgtgttgtg gtaactctat
     2161 tctacagaag ggacttcctt ttttctattt acacaaaata ttttacactc tcaacaaaag
     2221 tatcttcgat taatacttgc tcaagttatg tgcagctacg aaaaagtagg taccgaggta
     2281 ctttcttttt cgcagctttt tttggactta tgagaagtac attcgctgga agaatacgat
     2341 ataaaacgag ctttttcact aaagatatta atagagtgaa agaaacaagc ttttgttgtc
     2401 agttttttca cttttctaaa aatgaaaata aaaaattctg gaagtggaga agaattttgc
     2461 ttgctatact ttgtcttgta gaaaagaggt agacaaaata atgcaagctc aaataagcga
     2521 ttttccataa ccttggtagg aatgacaaat taaataggaa agggaagtaa taatatgaat
     2581 aaaaaaaagt tatctctatt cggttttttt gccctgacag cttccatggt tttgactgtt
     2641 tatgagtatc cgacctttgc aacatcacaa ttacatttgg ttttctttct tcttttggga
     2701 ggtattttat ggtttctacc agttgcttta tgtgctgcag aaatggcaac agttgaagga
     2761 tggaaagatg gtgggatttt cagttgggtt agtcaaactt taggagaacg ttttggtttt
     2821 gcagctattt ttttccaatg gtttcaaatt acagtaggtt ttattacaat gatctacttc
     2881 atactaggag cgctgtcgta tgtaattaat tttccagagc ttaatacaga tccatttatc
     2941 aagtttattg gtgtattagt cattttctgg gtactgactt tttcacagtt gggcgggacc
     3001 aatcgtaccg ctaaaattgc taaagcagga tttgtcattg gaattgttat tccttcgatc
     3061 attctctttg gtttagcagc agcctacttt atcggcggta atccgattca aatggctatt
     3121 aatgcgaaag cttttattcc tgacttttct cagctttcaa ctttagtagt atttgtatct
     3181 tttatattgg cttatatggg cgtagaagct tcagcatcac atattaatga gctggaaaat
     3241 ccccaaagaa actacccatt ggcaatgttt ttactagtga ttttggccat cgccttagat
     3301 gcaatcggcg gtttctcagt agcagcggtt attccacaac atgacttgtc tctcagtgct
     3361 ggtgtaatcc aaacatttga aacgcttatt ttacatttta acagtcattt acattggttg
     3421 gtaaaagtga ttgctctcat gattgcctgt ggcgttatgg gtgaagttag ttcttgggta
     3481 gttggtccat cacgagggat gtttgcagct gctcagcgag gtctgttacc agttttcttc
     3541 cggaaaacta ataaacaaga cgttccagtt ccgttgatta tgatacaagg aattatcgtt
     3601 actatttggg cagcagtctt aacttttggt ggaggaggca acaatttatc attcttagta
     3661 gcaatctcct taactgttgt catttatctc gtaggatatt tactattttt cctaggttac
     3721 tttgtattag tttttaagaa aaaagactta aaacgaactt acaatgtacc aggaaaaact
     3781 gttggcaaaa cgattatcgg tggaattggt tttattttat cagtgttcgc attgcttatc
     3841 tcttttgtac caccagcttc aattgcagct agtgagcacc gaacatacca aatcatacta
     3901 cttattagct ttgtagtgac tgttctttta ccttttatca tttatgaagt acatgataaa
     3961 agtgctcaca caactattga ggaacctact cacgtgaaag ctgaagacgt taatcccgct
     4021 gtttatccag ctgctagagg agagcaccac gttacaaaaa atgatgaaca tattttaaaa
     4081 cattaggagg aattcattat gttatacgga aaagataatc aagaagaaaa aaactatttg
     4141 gaaccaattt ttggctctgc aagtgaggat gttgacttgc ctaaatataa gttaaacaaa
     4201 gaatccattg aaccacgaat tgcttatcaa ttagtacaag acgagatgtt ggatgaagga
     4261 aatgcgcgat taaacttagc tactttttgt caaacgtata tggaacctga agcagtgaaa
     4321 ttgatgaccc aaacgttaga aaaaaatgca attgataaat cagaataccc acgaacaacg
     4381 gaaattgaaa accgctgtgt aaatatgatt gctgatttat ggcatgctcc aaataatgaa
     4441 aaattcatgg gaacttcaac gatcggctct tcagaagcct gcatgctggg tggtatggcc
     4501 atgaaatttg cttggcgtaa acgtgctgaa aaattaggtc ttgatattca agcaaaaaaa
     4561 cctaacctgg tgatctcttc tggttaccaa gtttgttggg aaaaattctg tgtatattgg
     4621 gatgtggaac tgagagaagt cccaatggat gaaaaacata tgtcaattaa tctagatact
     4681 gtcatggatt atgtggatga gtacacaatt ggtattgtag gtattatggg tattacttac
     4741 actggtcgtt atgatgatat caagggtctg aatgatttag ttgaagctca caataaacaa
     4801 actgactata aagtatacat tcatgttgac gctgcatcgg gtggctttta tgcaccattt
     4861 actgaacctg atctagtttg ggattttcaa ttgaaaaatg ttatctcaat taattcttca
     4921 ggtcacaaat atggtttggt atatccaggt gtgggttggg tcttatggcg tgaccaacaa
     4981 tacttaccag aagaattagt atttaaagtt agttacttag gtggagaaat gccaactatg
     5041 gctatcaact tctctcatag tgcagcacaa ctaattgggc aatactacaa ctttgtacgc
     5101 tatggctttg atggttatcg tgatattcac caaagaactc atgatgttgc tgtttattta
     5161 gccaaagaga tcgaaaaaac tggtattttt gaaatcatta atgatggatc agaattgcca
     5221 gttgtgtgct ataagctgaa agaagatccc aatcgcgaat ggacactata tgatttatct
     5281 gatcgtctgt taatgaaggg atggcaagtc ccagcctacc cattacctaa agacttggat
     5341 caattaatta ttcaacgctt agttgttcga gcagactttg gaatgaacat ggctggtgat
     5401 tatgtacaag atatgaacca agcaattgaa gagttgaata aagctcatat tgtttatcat
     5461 aaaaaacagg atgtgaaaac gtacggcttt actcactaaa attcttcaga gaaatataaa
     5521 agaagttaat taactaataa aaggagttgt gattaccaca cagctcctta aatctatttg
     5581 aataaatata tgatattcat ttgttacact ctcaatctta gctgttgttc tattcatcat
     5641 tactcttatt gtaagtagtt cagtcaaatt tgcttgttca attgatcgat aacagtatga
     5701 attgtttgaa aagtaatcgg atatataatt gttttctcac tacctaagcc ttcttggtat
     5761 aaagaactat cgcaaagtat aatccgctga gtacaaaaga aagtgtcgtc tactatttct
     5821 ctaagttgtt tacaaatgat attactaata gaaaagtgtt ctcccaaaat gtcttctaac
     5881 tgttgtcgat agtataaata cctaaagtct gacggagcta ctatgaacac ttcaattgga
     5941 gatttccgtt tatgctcatt taaaagactt aaactaatgg ttagtttacg aatagaattc
     6001 tcattccaac gcagaaataa attgttttgt tttcgccatt gttcaagaat ttcatgaact
     6061 tctaagtaca aagcgcgttg ctcttcattt aacaagtaag tcttatcagg ttgaaatatc
     6121 tgaatatcag cccaaagtga gcggataaac gggagaagtg ctttatcaaa aagtatctga
     6181 ttttcttcag atatattcag agataagata agtttttgac tgagctcaat caccaaaggg
     6241 ttatctgcta catggttttg ataaacaacg ttcaagtctt tttgtaacaa ttcaaagtta
     6301 tcgcatagat aatttctaga gttgaataga aagtagatat aaaggcattc atcttcgtca
     6361 tgacataaag agaaagaaag ttgctctaat cctttctgta ttagctgaaa caagggagta
     6421 tgtcgggcaa ctcttttttc ttcctgacta aaatatattg ggttagtctt agatctctgc
     6481 aaaccgatag cgatacctaa taaaataaat tgtttttcaa ctgtagggta acttcttgaa
     6541 aaaaatgttt ttctacataa tcaatcaatt gttgaccgct ttgaataatt gatttattta
     6601 gctggatcaa ctctgtccga tttgtctcat agattatcgt tagtaacaaa cttcgataat
     6661 ccttttcagg aatttcaaaa tggccatcag catttaagta ctgcatttgt ttaaaaaaat
     6721 taatcaactc atttcgtaag ttgtaaactt tgcttaacga aatgaaggta gtatcagcta
     6781 tattactcca attgtgttcc cctgataaat aatgtcctag tacattgaaa aagtcagagc
     6841 gctgatagat gagttgagtg agtgccaacc tcgaaaacga agagtcgaat gcgactgtaa
     6901 agctacttct agatttctcc actgaaataa tttgctgatg gagtatgtcg gtaatgcatt
     6961 ccaagtcgtt agtgacagtg agtgggcaaa cgttcaattg atctgctatt tcgcgaatct
     7021 gaatggtccc tttttcgtgt aattgctcac aaatgtaaac ttgacgaaaa atacttttct
     7081 ccagataggt ttccaacata gtactcctcc tagtcatttt atacataact attataactc
     7141 ta
//
LOCUS       DAZBSA010000122         6644 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000122 DAZBSA010000000
VERSION     DAZBSA010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.123"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACHGMT_002508"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACHGMT_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACHGMT_002509"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACHGMT_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFN6836218.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACHGMT_002510"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACHGMT_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACHGMT_002511"
     CDS             complement(2143..2373)
                     /locus_tag="ACHGMT_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFN6836219.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACHGMT_002512"
     CDS             complement(2603..3421)
                     /locus_tag="ACHGMT_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN6836220.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACHGMT_002513"
     CDS             3582..4271
                     /locus_tag="ACHGMT_002513"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFN6836221.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACHGMT_002514"
     CDS             4285..5787
                     /locus_tag="ACHGMT_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN6836222.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ACHGMT_002515"
     CDS             5800..6276
                     /locus_tag="ACHGMT_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFN6836223.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ACHGMT_002516"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ACHGMT_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2236 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DAZBSA010000123         6517 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000123 DAZBSA010000000
VERSION     DAZBSA010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.124"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            116..889
                     /gene="sufC"
                     /locus_tag="ACHGMT_002517"
     CDS             116..889
                     /gene="sufC"
                     /locus_tag="ACHGMT_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFN6836224.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            902..2188
                     /gene="sufD"
                     /locus_tag="ACHGMT_002518"
     CDS             902..2188
                     /gene="sufD"
                     /locus_tag="ACHGMT_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFN6836225.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            2185..3420
                     /locus_tag="ACHGMT_002519"
     CDS             2185..3420
                     /locus_tag="ACHGMT_002519"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFN6836226.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            3407..3877
                     /gene="sufU"
                     /locus_tag="ACHGMT_002520"
     CDS             3407..3877
                     /gene="sufU"
                     /locus_tag="ACHGMT_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFN6836227.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            3882..5273
                     /gene="sufB"
                     /locus_tag="ACHGMT_002521"
     CDS             3882..5273
                     /gene="sufB"
                     /locus_tag="ACHGMT_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFN6836228.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(5371..6513)
                     /locus_tag="ACHGMT_002522"
     CDS             complement(5371..6513)
                     /locus_tag="ACHGMT_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6836229.1"
                     /translation="MATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILP
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
BASE COUNT         2082 a         1259 c         1398 g         1778 t
ORIGIN      
        1 aaaattgttt aataataaat aaaagatatt tgattttagt aaggaaggtc attgcgcttc
       61 attctcaatt agtctaaaat gagtgagaac gaaagagaat ttgggggtat aatgcatgtc
      121 tgtcttagaa ataaaaaatc tacatgtgtc gatcgaggaa aaagaaatcc ttaaaggcgt
      181 aaacctaaca atgaaaaccg gagaaatcca tgccatcatg ggacctaacg gcacaggaaa
      241 atctacctta tctgccgcta tcatgggcaa tccaaactat gaagtaacag aaggcgaaat
      301 tttgtttgac gggaaaaatg ttttagactt ggaagtagat gaacgtgcgc gacttggctt
      361 atttttagct atgcaatatc caagtgagat accaggtatc acgaatgcgg aattcatgcg
      421 tgcagcgatc aatgcgaaac gtgatgaaaa cgacaaaatt tctgttatgg catttttgaa
      481 aaagttagat gaaaaaatgg ctttactaaa tatgccagaa gaaatggctg aacgttatct
      541 gaacgaaggt ttttctggcg gagaaaagaa acggaatgag atcctgcaat tgttgatgtt
      601 agaacctaca tttgcaattt tagacgaaat cgattcaggt ctggatatcg atgctttgaa
      661 agtcgttgct aaaggagtaa atgaaatgcg cggagacaat ttcggtgcat tgatcatcac
      721 ccactatcaa cgattattga actatatcac acctgatgtc gttcacatca tgatggaagg
      781 ccgagtagtc atgacaggga atgctgacct agctaaacgt ctggaagcag aaggctacgc
      841 tggtatcagt aaagaattag gaatcgacta taaagaagaa gaagcgtaag ggggagcaag
      901 aatgaaagaa aaaaactggc tcgattatct tgacgcggta aatgattttt ctttaagtaa
      961 aggcgaacct gattggatga gaaccttccg tcaagacgca ctagcgaaag cagatgaact
     1021 gccattgccg catatcgatc gggtgaaatt ccatcgctgg tcgttatttg atgtgaaaga
     1081 aacacaaacc atcagtgaaa caggcacgat tccggcattt gatgcaatga aagacaatcc
     1141 tgtgcttgtc caacaaggtt catggacgat ttttgaacaa ctgcctgtgg aattagcaga
     1201 aaaaggagtc atttttaccg acttgttcac tgcaatgatc gaatatccag aactagtcca
     1261 agaatattat atgaaaaaag cagtaaacat gaacgaagac caattgactg cactccatgt
     1321 agcttttatg aacagcggta ttttcctgta tgtgcctaaa aatgtcgtga tcgatgaacc
     1381 actcgagtcc ttattcattc aagatggcgc atccgatgag cacttcttca agcatgtgtt
     1441 gatcgtggca gatgaacata gtgaattttc ttatctggaa cgtttccaga caaccaaaga
     1501 acaagtagca aaatcatcag gtaatatcat cgtggaagtc atcgctaaag ctggttcaaa
     1561 aatcaaatat tcagccgtag atcaattagg agaaaatatt acttcttata tgaatcgccg
     1621 tgggcacatt ttacgagatg cttctgtcga ctgggcaatt ggtgtcatga atgacggtca
     1681 tgtgatcgct gactttgact cagatttagc aggagaaggt gcacacgcag aagtcaaaat
     1741 cgtggcaatc agctcgggca gacaaatcca aggaatcgat acacgagtaa ccaataaagc
     1801 ccctcatacg atcgggcata ttttgcaaca cggagtgatc cgagaaaaag gaacattgac
     1861 ctttaatggt atcggccaca tcttgaaagg ggcaaaaggt gcagatgccc aacaagaaag
     1921 tcgtgtcttg atgctttctg ataaagcacg tggtgatgcg aatcctattc ttttgatcga
     1981 tgaaaacgaa gtaactgctg gacatgctgc cagtgtagga cgagttgatc cagaagaaat
     2041 gtactatctg atgagtagag gattgcataa agaagaagca gagcgcttag tgattcgagg
     2101 attcctcggt tctgtcttaa cagcaatccc tgtagaacaa gttcggaaag aattagtcga
     2161 agtaatcgaa gggaagttga atggatgatc gatgctaaaa agctacgcaa tgattttcct
     2221 atcttatttc aagacgtcaa tgatgaacct ttagtttatc tggataatgc agcgacgacc
     2281 caaaagccaa aacaagtact ccaggcatta gcgtactatt acgaacacga taacgccaat
     2341 gttcatcgcg gtgtccatac gttagcagaa cgcgcgaccc atgattatga acaagcaaga
     2401 gaaaaagtac gggcgttcat ccatgcaaaa gaaacagcag aagtgctgtt tactcgtggt
     2461 acgacgacaa gcttgaattg ggtagccaga accttcggcg accgctatat caaagcagga
     2521 gacgagatag tgctctctta catggaacat cattctaatg ttatcccgtg gcagcagttg
     2581 gcaaaacgaa caggtgctgt cttgaaatat atcgaactaa cagaagaagg cttcctggat
     2641 atggaggatg cagcgaaaaa gattacggac aaaaccaaaa tcgtctccat tgctcatgta
     2701 tccaatgtct taggtgtctt caatcctgtt caagaattag ctgaacttgt ccatgaaaac
     2761 ggcggggtca tcgtagtaga tggtgctcaa tctgccccgc acatggccat tgatgtccaa
     2821 gcactagact gtgatttctt tgctttcagc ggacacaaaa tgtgcggacc aacaggtatt
     2881 ggtgttttat atggtaaacg aaaatggtta gaagaaatgg aacctgttga atttggtggc
     2941 gaaatgatcg attttgtcga attatacgac agtacatgga aagaactacc ttggaaattc
     3001 gaagcaggga caccaaatat cgccggcgct atcgcattag gccatgcaat cgattatttg
     3061 caaaaactcg gtatggacaa tatccatcga tatgaagaag aactagccgc ttatgtgttg
     3121 ccaaaattac aagcaattga tggattgact gtttatggtc cacaagaccc gaattttcgg
     3181 acaggagtcg ttgcattcaa tttggatggg ttacatcccc atgatgtcgc gacagcttta
     3241 gacatggaag gggtagccgt cagagcaggt catcattgtg cgcaaccgct attgaagtac
     3301 cttgaagtat cagcgactgc tcgcgcgagc ttttatttct acaacacaac gcaagacgcc
     3361 gaccgtttag ttgaagcaat catggcaaca aaggagtttt tccaacatgg gactatctaa
     3421 attagacaat ttataccgtc aagtgatctt ggatcattct tcccatccgc atcatcgtgg
     3481 aagcttgact gactcgacaa gcaagatcga attgaacaac cccacatgtg gagatgtgat
     3541 cgaattgcaa gtcgatgtaa aagacggaat catcaaaaat atcgcctttg acggcagtgg
     3601 ctgctccatc agtacggcaa gtgcctcgat gatgacagac gccgtcatcg gaaaatcaat
     3661 agaagaagca gaacatttaa ctgtagactt tctacagctt gtccaaggaa aagaagtcaa
     3721 agatcccgac caattaggcg atgctgctat gcttggtggt gtagctaaat ttcccgctcg
     3781 gatcaaatgt gcgacacttg cctggaaagc actaggaaaa gcaatggaaa acgatggtac
     3841 cgcagctttc catcattacc acaaggaaga ggagtgagaa catgggcgta ccagaattag
     3901 aagaatataa attcgggttt cacgatgatg tcgaaccggt tttcagtaca ggagaaggct
     3961 tgacagaaga agtgatccgt gagatgtcaa gaatcaaagg cgaaccagaa tggatgcttg
     4021 aattccgtct gaaatctcta gaaacattta acaaaatgcc aatgcaaaca tggggccccg
     4081 acctttcaga catcgatttc gatgccatca aatattacca aaaaccaagt gacaaacccg
     4141 ctcgtgactg ggaagacgta ccagaaaaaa tcaaagatac atttgaaaag attggtatac
     4201 cagaagctga acgagcttat ctagccggtg cttccgcaca atatgaatca gaagtcgtgt
     4261 accataatat gaaagatgaa ttcgaaaagt taggaatcgt ctttactgac actgactctg
     4321 ctttgaaaga ataccctgaa ttattcaaag agtacttttc aaaacttgta ccgccaaccg
     4381 acaacaaatt agcagcgctg aattcagccg tatggtcagg tgggaccttt atttacgtgc
     4441 caaaaggcgt caaagtcgat gttccattac aaacgtactt ccggatcaat gcggaaaaca
     4501 cggggcaatt cgaacgtacg ctgatcattg ttgacgaagg agcaagcatc cactatgtcg
     4561 aaggttgtac ggcacctact tattcaagta acagcctgca tgccgccatc gtagaaatct
     4621 ttacaagaaa agatgcgtat tgccgttaca caacgatcca aaactggtca gacaatgtgt
     4681 acaacttagt aaccaaacga gcaaaagcat acgaaggcgc aaccgttgaa tggatcgacg
     4741 gaaacctagg tgcaaaaaca acgatgaaat acccaagtgt ttacttagat ggaaaaggtg
     4801 cacgaggcac catgctctcc atcgcctttg caggagcaaa ccaaatccaa gatacgggtg
     4861 caaaaatgat ccacaatgca ccaaatacat ctagctctat cgtctctaaa tcaatttcaa
     4921 aagacggcgg agaagtaaac tatcgaggac aagtaacctt tgccaaaaac agtgcgggtt
     4981 cgatctctca tatcgaatgt gacacgatca tcatggacga atggtcgaaa tccgatacga
     5041 tcccattcaa tgagatccac aacagccaag tctctttaga acacgaagcc aaagtctcaa
     5101 aaatctcaga agaacaactc tactatctga tgagccgagg cctgaccgaa tctgaagcaa
     5161 ctgaaatgat cgtcatgggc tttgttgaac cattcaccaa agaacttcca atggaatatg
     5221 cagtcgaact caatcgattg atcagttatg agatggaagg gtctgtaggt taataactat
     5281 ttcattgctc gaaacgttga tattaaagcg tttcgagcgt ttttttattt gtattttaat
     5341 ggttggctac gtttttggct acgtttggaa ttattcgccc ataaattttg caaatcggtc
     5401 accagttttc ttaacagatt gtggtgtgac atgagcatag atattcatgg tagttttaat
     5461 atctttatga cctaatcgtt cctgtacttc ttttatcgac gcaccacttt caaacaataa
     5521 gctacagtgg gtatgcctaa atccatgtgg agtaatttga ggctccatcg gtgtcttttt
     5581 gtaaatccag tctaaccaat cgtttactac ttgggggtag tacaacttgt ttgtgtttgt
     5641 agtgaaaaga aactgttttg gtgaattcgt gttatagcca tataagatca ttattttcat
     5701 ttgttcttgt tgccaaatac gcagttgttt aagtgtaaaa tcatcaagct gaatcgttct
     5761 tgatgaattt ttagttttag gtacttgtag aactacttga ttaaactcgt cttgagcgat
     5821 agttttacca attgttattg ttttattgaa atagtctata tcttcccatt gaagcgccaa
     5881 tgcttcgctt ttacgcattc ctgtataagc taataaacgg aaaaaagtgt acagtttaac
     5941 tgaattattt tctttagtat tctgtaaaaa cagttgtaat tgatctttcg tgtaaaactt
     6001 caagggttgt tcttcttcaa tttttcttgg aagcaatgtt ttagacatag gattatcctt
     6061 acagacttcc attgctactc caaatttaaa tatttgagca gttgtttttc tcaagaaacc
     6121 atattgtttt aatgttttgt gccattcatt cacctgtttt tggcaatagc taacacttat
     6181 tttatccaat cgtaattccc caaaagcagg taatatatgg tttttgcaaa aacgttgagc
     6241 aacggcaaca gaagacggtt ttactcttgt tctgtagtta tcaatccata attcatacaa
     6301 ctcttgaaaa gttgtaacat tgattttaaa ctcttgtttt tctttaaagt ctacaagtag
     6361 tctattaagt gctgtttgcg cctcagcttt tgatttgaat cttctacgct ttgtacgtat
     6421 atcttttcca gtcttgggat ctttccctaa ataagtttca aatccccaat atttttcgcc
     6481 attttgtaat ttataactaa aaaatgttgc catttga
//
LOCUS       DAZBSA010000124         6405 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000124 DAZBSA010000000
VERSION     DAZBSA010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.125"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACHGMT_002523"
                     /pseudo
     CDS             complement(99..1229)
                     /locus_tag="ACHGMT_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACHGMT_002524"
     CDS             complement(1878..3080)
                     /locus_tag="ACHGMT_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6836230.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACHGMT_002525"
     CDS             complement(3140..3343)
                     /locus_tag="ACHGMT_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFN6836231.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4552)
                     /locus_tag="ACHGMT_002526"
     CDS             complement(3485..4552)
                     /locus_tag="ACHGMT_002526"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFN6836232.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACHGMT_002527"
     CDS             complement(4610..4717)
                     /locus_tag="ACHGMT_002527"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6836233.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACHGMT_002528"
     CDS             4880..5692
                     /locus_tag="ACHGMT_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6836234.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6257)
                     /locus_tag="ACHGMT_002529"
     CDS             complement(6066..6257)
                     /locus_tag="ACHGMT_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836235.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1919 a         1310 c         1007 g         2169 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagctaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgcc
//
LOCUS       DAZBSA010000125         6307 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000125 DAZBSA010000000
VERSION     DAZBSA010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.126"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..340)
                     /locus_tag="ACHGMT_002530"
     CDS             complement(<1..340)
                     /locus_tag="ACHGMT_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN6836236.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVY"
     gene            complement(460..1107)
                     /locus_tag="ACHGMT_002531"
     CDS             complement(460..1107)
                     /locus_tag="ACHGMT_002531"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFN6836237.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(1191..2618)
                     /gene="uxaC"
                     /locus_tag="ACHGMT_002532"
     CDS             complement(1191..2618)
                     /gene="uxaC"
                     /locus_tag="ACHGMT_002532"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFN6836238.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(2692..3771)
                     /gene="uxuA"
                     /locus_tag="ACHGMT_002533"
     CDS             complement(2692..3771)
                     /gene="uxuA"
                     /locus_tag="ACHGMT_002533"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFN6836239.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(3774..5405)
                     /locus_tag="ACHGMT_002534"
     CDS             complement(3774..5405)
                     /locus_tag="ACHGMT_002534"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFN6836240.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            5715..>6307
                     /locus_tag="ACHGMT_002535"
     CDS             5715..>6307
                     /locus_tag="ACHGMT_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN6836241.1"
                     /translation="MKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRTPI
                     REALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQKK
                     RILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERFRW
                     LRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTS"
BASE COUNT         2013 a         1279 c          871 g         2144 t
ORIGIN      
        1 tgtatactac cttaacgctc atagaagctg aaatcataga agtttgatac attaaaaaac
       61 ttgctaaagc gactggacca ctcattctta aaatccacgg tttaaactta ccctgcttag
      121 ttgctttcga tctatcaaca atacgcccca tagttacatc agtaactgca tcgataaatc
      181 ttgctactaa aaataatgtt ccaacggcag ctccgcttat ccccaatact tttgtataaa
      241 agatcattaa aaaagaacta gcaaaaatga aagtaaaatc gtttccaaaa tctccaaaca
      301 tatatcctat tttgtctctt ataccaaatt ttctttccat acaattctct cccttctgtt
      361 gttaagcgtt ttcattctga tgaaagtacg tactatactc tgaagcgtaa agagtcagta
      421 tttatttttt acatttcaga gtattatttg ttttttctcc taataattct ttatctcata
      481 aaacttatct atatatttac tagctactcg acttacttct tcaaaatcac ctttttcaac
      541 agaagccaat aaattcccac ctacaccaac tgcaacaact cctacttcaa accaatcttc
      601 taagttctct atacttactc cgccagtagg cattatattt acgtgtggta atggagcttg
      661 ataggctttt acaatggatg gaccaaaagc agaacctggg aatagtttaa tgatatctac
      721 gccagcagta agtgcctcct ttatctcagt aattgtcata caacctggta aataaggaat
      781 ttgatataaa ttacaaatct cagctgtttg tcgatcaaaa gatggactaa cgatatattc
      841 tgccccagcc ataattgcga tacgagcagt aaacgcatct aaaaccgtac cggctccgat
      901 tattacatct ttttgatcag catatttttt agcaatttct ttgattattt cttcagcatt
      961 aggaactgta aaagttagtt caatcccttt aattccaccc gcaataattg aatttattgc
     1021 tttaatggcc tcatccttat ttttcccacg tacaaccgca atgactccag ccgcttctaa
     1081 cttacttaaa atttctactt ttttcatatc tatctccttc ctttatggat acatactttg
     1141 tctagctgta ctaacttaca aagaataaat ttaagctttt atttcaagta ttattttcta
     1201 cttccgattt caaaaaaatt aaaataatta tacgcattac gataacaaat attctccact
     1261 atctgactaa gatacatttc atcatctgga attcggcctg ctttaaccca ctcgcttagt
     1321 aattgacata atactcgacg aaaatactca tggcgaggat agcttagaaa acttcgagaa
     1381 tcagtcaaca tacctgtaaa attcgacaac aaactttgtt gtgccatcgt agtcaactgt
     1441 tgacgcattc cttcagctgt atcattgaac caccaagcac atcccaattg gattttttga
     1501 atcatacccc cttggaagct ttgcattgct gttgctagtg gaagccaatc agttggattt
     1561 aatgaatata aaattgtttt tggtaagcac ccactctttt cagcggtatc gaacaatgct
     1621 acaacatttt gtgtcaaact agcttgatcc cccatcgaat caccaccgac atctactcca
     1681 atttgttcct ttaatatact tgaattattt cttaaagcat ttaaatgcat ttgcatcgtc
     1741 caaccatatc ttgtatactc agacatcaaa tgtaattgaa tacctgtaac aaattgtgca
     1801 atttctgata aatggaaatc cgtttttcct gctaaagctt ttattaacag attgtcgagt
     1861 attttttcat ctgctatttc ataaaaataa gtattcaaac cgtgatctgc taatcgacag
     1921 ccaatttgat gaaaatgatc cactcgttgg ctcaatgctt gcttcaaccc attaaagtct
     1981 ttgattggta tagccgctgt ttgactaagt tttaaaatat agttcgaaaa gcctgatgat
     2041 ttgatactcc agaccttatc tggacggaaa gtaggtaata ccttgaaaga attatcctgt
     2101 ttagaaagct cagtatgata ccacaagtca tctgtaggat catccgttgt acagatggct
     2161 ttcacatcca tcatctccaa caattctttc gggcgatagg aatccgtttg aattttttcg
     2221 ttagcacgtc gccaaatttc tggtgcattc ttctcagaaa ttgtcaaatc aatatcaaag
     2281 taacgttgta attccaaatg agaccattca aaaataggat tacctggcgc cttctccact
     2341 gtttttacaa aagctaaaaa tttttcataa tcatcacctg gaccactgat caactcttct
     2401 tctataccat tagctcgcat caatcgccat ttataatgat ctccagctcc tttatcattt
     2461 aaccatacct gagtcaaatt cttgtattgc ttattttcta gcaaatatgc tgggtctaaa
     2521 tgacaatgat agtcgataat tggttggtgt tttgcaaatt tatgaaaaag tcttttagct
     2581 atttcattct ctaataaaaa gttcttatct aaaaacatat ttaaacactc ctttccttcc
     2641 gttagttttt agaagagaaa atttttcctc taaataaact cccttatata attatttact
     2701 cgcccccttt tcaagcgctt cccaaatacc atttaaataa actgcaccta gagctcggtc
     2761 atataatcca taaccaggtc taccggtttc tccccaaatc atccgaccat gatctggacg
     2821 aatatagcca ttaaatccat tatcgtacat tgtttttaat atcttataca tatctagaga
     2881 accatattct gatttatgcg gcgcttcgta aaaatcgagt ccattgtcac ttaaatattt
     2941 gatattacga acatgtgcaa aaggaatccg atcccgttga caaaattcag atagaatttc
     3001 atagacatca ttatctggtc gttctgcaat acttccagta cataatgtaa taccattaga
     3061 aggagaatcc actacattac acaaccaatc taaatcgtca cgatttttaa caatacgagg
     3121 taatccgaaa atggaatatg gtggatcatc tgggtgaact gccattttaa tcccaacttc
     3181 ttcacatgtc gggataattg ctttgagaaa atatgcaaag ttctctcgta atttctcttc
     3241 atcaacttct ttataggctt ctaataattg cttaatttgt gataaccgat gaggttccca
     3301 acccggcaat tcaaaattat tagtagaatt ttctacttct ctaataattt cctccggatc
     3361 agtagggata ttttttgaaa tataggctaa cgtagaagac ccatcatcta aacgataatc
     3421 caaatccgat tttacccaat caaatactgg cataaaatta taacagataa cctcaatccc
     3481 aaattctgcc aaattgcgaa tcgtctgttt ataattctca atgtattgat ctcgtgttgg
     3541 taaaccaatt ttgatatcat catgaacatt aacactctca ataactttca aggtaagtcc
     3601 attggcttcg acttcatctt ttaaagattt aattcgttct ctcggccaaa cctctccggc
     3661 tggtatatcc attaatgttc caacaattcc tttcattgct gggatctgac gaatttcttg
     3721 tagtgagatt ttttcttcat ttgccccata ccatctaaaa gtcatttcca tagttacttt
     3781 cctccaactt ctgttatata tttattgatt gttttacgaa ctgcttgagg acctactagc
     3841 atttcgcaaa agattttttc agttttcttt cctaccccaa cttgatataa atctaaacca
     3901 aagatttctt tattttctaa aatttcctgt accgcacaat gaatctttga aggatcttgt
     3961 tcacctaatt tcaatactga taatttatcc tgcaactcta ccaataacgg atcaggactt
     4021 ggcacaaaat tttccccttg atcatcgact gccagtaaat aacgtaacca agcagcaata
     4081 gcgataggaa taaatgttag attttctgca tttccgtctt tttcgacata ctttttaatt
     4141 gtttcaccat agcgaatcgc tactttttga gaagtatctg ttgcaattcg ttgtggtgta
     4201 tctggaatat agggattagg caaacgttta ttaattactt catcaataaa ttgttttgga
     4261 tcaataatgc ctggattttc aactactggt aaaccttcta aataacctat tcctttgatt
     4321 aaaccaacca aatcatcatc ctgcatctct gctgaaattg attgatatcc tagcagacaa
     4381 cctgttactg ccaaagcagt atgaagtgga ttcaagcttg tcgtaacctt catgatgtct
     4441 gctttatcaa ccgtctctct atctgtcata atcacaccag ccttttccaa tttaggccgg
     4501 ccatttggaa aactatcttc aataactaaa taatgaacta cctcagtatt agtgaaagca
     4561 gcaatattgg tatgtttttt tgtgtgaatg atatctaaat cctcgagccc catcatcaat
     4621 agatcttcct ttacattctc tgaaggattt ggcgtaattc gatcaatcat actccaagga
     4681 aaagtcactt tttctttatc ctttaaataa gcaataaatt tttcagaaac atatccttct
     4741 ttttgccaag cttctgcaat cgtaaaaata ctatcttgaa aacgttgacc attctgtgag
     4801 aaattatcag tagaaaccat cgcaatcggc aactctccag cattaaatct gctatataac
     4861 aaggatgtta caatactcat cgtatgttga ggattgctag gtccagattt gatatcagct
     4921 ttgactaccg aaaagaattg ccctttagaa tcttttaaac tgtaaccttt ttcagtaata
     4981 gtaaacgttg taaactgtaa tttaggatct tcaaaatatt taaccactct ttgatagtcc
     5041 gctatattct tcggattgca atataaacta tcagccgtta ctgccaaaag tctttttttg
     5101 agttcaccat ctccactcat aacaatttcc aaaatgtcat tgttatagtc atgataagct
     5161 ttttccacta cttcttcatc aaatgtttca cacacaacta ctcctgtttc taattcattt
     5221 tgatctgcta agcattgagc aatttctgca tgaaatcctc gataaagatt tcctccacca
     5281 aaatgaagcc atactggatg ttgctggcct tcttgcttaa cttctttgac atcaaaacac
     5341 ggcagtagaa tctgcatttc attgaattct tcgatagaat tttgccaatt tttaatcgaa
     5401 accataatta acctcctcaa actgatatgt tagttttgat acattttata ttttaaaacc
     5461 cattgtaacc gttgtcaata taaaaaggga ataaacttgc ttttttctac catatcagaa
     5521 tgattgtatt ttttattcag aaaaataaaa tactccttct ttttggactg attttatcga
     5581 aacttatttt ttcttttttt catgtattct tttattttta taactagtgt attacttcaa
     5641 aatttgatat tatatggcat gaacagaatt aaaatatcag ttaaaataaa agttaaggag
     5701 cattgtgtat gacaattaaa actagtctcc aaaatcaagc ttaccaatct attcgccgac
     5761 aaattatcta tgctgactta gaacctggaa aaaaagtatc agaaaaaaaa ttagaagaaa
     5821 cactttctat tggtcgtaca ccaatacgtg aagcactgat tcaactccga caacaaggtc
     5881 ttgttgatac aattcctcaa agcggaaatt atatctcatt aattgattta gattcggcca
     5941 aaaatgctcg atttgtcaga gaacatttgg aacgacaaat tatgttagag tgttgtgcca
     6001 aaatgaataa ccaaaaaaaa cggattttag aaactataat ttcagaacaa gaaaaagctg
     6061 ttagtcaaaa aaatgctcgt agtttttttc ataatgataa tttattccat aaagcttgtt
     6121 ttgaaattgc agagcgagaa gaagtatggc aatggttaga aaataacaat acacacttag
     6181 aacgatttcg atggctacga gtaaaaacgg aaggattaaa gtgggaaaaa attatggaac
     6241 aacattatca actatttgag gcattaacaa aacaagatcc agaagaagct aactttctaa
     6301 catcgtt
//
LOCUS       DAZBSA010000126         6302 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000126 DAZBSA010000000
VERSION     DAZBSA010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.127"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(63..308)
                     /locus_tag="ACHGMT_002536"
     CDS             complement(63..308)
                     /locus_tag="ACHGMT_002536"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6836242.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(302..703)
                     /locus_tag="ACHGMT_002537"
     CDS             complement(302..703)
                     /locus_tag="ACHGMT_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFN6836243.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(890..2698)
                     /gene="uidA"
                     /locus_tag="ACHGMT_002538"
     CDS             complement(890..2698)
                     /gene="uidA"
                     /locus_tag="ACHGMT_002538"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFN6836244.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(2721..4487)
                     /locus_tag="ACHGMT_002539"
     CDS             complement(2721..4487)
                     /locus_tag="ACHGMT_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFN6836245.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(4500..5294)
                     /locus_tag="ACHGMT_002540"
     CDS             complement(4500..5294)
                     /locus_tag="ACHGMT_002540"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN6836246.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(5304..>6302)
                     /locus_tag="ACHGMT_002541"
     CDS             complement(5304..>6302)
                     /locus_tag="ACHGMT_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN6836247.1"
                     /translation="TSINIPYGSMASAITSITEERTMLSTFRNVGATLASLVIGVLTP
                     LFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRIDQSQYKVSLL
                     KSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIVLMNFINPILV
                     LIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLILSSVGYMGLG
                     VFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVGGWTLSIIGYD
                     SLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENCELLKQKHK"
BASE COUNT         2039 a         1222 c          777 g         2264 t
ORIGIN      
        1 ctaattcctt aactattaga agattctttt aaacagaaaa agtggaaagg ggattttttt
       61 aattagttaa ttttaaattt taagatcgct tctattaatt taatttctag ctgacttatc
      121 atatattcat ctacataggt aacgaattga cctacctcgt taaatccatt tgtcaaacat
      181 aacttagcca tataagaatg ataatgtttg agtatcatct tgatcgaaat tggatctcct
      241 tctagagcag ataagataat ggatagtgga actaataggt actgccgctt tttagattta
      301 tctaccatag gtcatacccc attttcaatt gttcaactgc cttctttcgt tgataccaga
      361 taccacttgt gctcatattg agaattttac cgatttcagc atcattaaaa cctgcaaaat
      421 aataaagtag gataatcttt cttcttatct ccgataactg atcaattgct gctgataact
      481 tttgatctga aattaatatt tccattccaa ataagaaata gacatcttgt tcatcaaaat
      541 gagtttggga atacctgcct tcatttgaat ctgctaatgg ttcctcatta tcctttatgt
      601 tagcgtttcg ttttcgaata gacttagctt cgtttcgtaa tactttttta cagtaggcat
      661 caaattggtg ttgtatagat atgtcattgt aatgttgttg catgataata tgctcctttt
      721 tccccctttc cactaataca acaatcagaa agcccctttt tttcgaaaaa actagaaaaa
      781 aaacataaaa atatttatca ttctttaaac agaaaatctt tctataaaaa acaaaaaaaa
      841 acgattaggc taggctaatc gttttttgaa cattcttaaa aaatccaaat tattttttat
      901 aactgaaatt gggtattgat gaccatcgtt tctgtagata gtaagcaatc attttaggtt
      961 ttctatctct agtaaagatt cctttcttat ttccttgcac tcttaaaatc ccttgacttg
     1021 ttgcaaaatc tgcaaaattc cacacttgtt caccaacaaa attttcacat gtatctatta
     1081 cttcgtgatt tgcttcgtaa taagccactt gatactcttc tgtaaacatt gttggtactg
     1141 tatcatgaaa tccagctact gtatctgctc catattctgt aaacattatt ggcgtatcag
     1201 gacatctttc catccatcca tctagctctt ttttcaacag atattttgca tgtgccagat
     1261 cccctcccga aacataccaa ccataataac gatttaaaca taaaacatcg acaatatctc
     1321 ctaccttaca aacatctggc gttgatttta gataagtaac gattgttaca ggtcttttct
     1381 gcggatcaag ttctttagtt aattcaacta aaggttcaaa atattcttta gcaccttcgg
     1441 aatccgaatc tggttcattt gcaatagacc acattacaac acttgggtgg ttcttgtcgc
     1501 gttctatcaa ttccttgatc accgttttat gagaattttc tgttcgtatc tctttccacg
     1561 tattatgttt ttccacatta tcatctaata aagtggccat gaaatttaaa tgtaacccta
     1621 ctgcaggtac ttcatcaata actacaatac cttctcgatc tgctaatcgc ataatttctt
     1681 ctgagtaagg ataatgtgat gttctaaaag aattagcacc tatccatttc attaaattaa
     1741 aatctttaat atttacagcc tcattcaaac cacgtccatt aacatacgaa tcctcatgct
     1801 taccaaatcc tttaaaataa aaaggtttat tattaatcaa aaatttgcca ttggtaacct
     1861 gaacactgcg aataccaaat tcttctgtat aggtatcgat cactttatta tcatcaatta
     1921 aactaatttt catactatat aaataagcgt ctagaggttc ccaaaaaatt ggattcttaa
     1981 gctgaatcat tcctttcttt ccttcagctt cagctacaag ttgagcctct tcgtcatata
     2041 tcgctattaa gatttttccg gtccattctg tatcgatatt gaattctaca cttgcatcca
     2101 aatcagttat tcttgtatta atagtaatat cacttatata gtctttcgga gttgtatata
     2161 acttaacagg tctatgaata cctgcataat tgaagaaatc aaaattaact aagttttcct
     2221 tttttatatc cccattttcc tgtactgttt ccttcataat ccccacaggt aaagtactat
     2281 cgtcaataat attgtttaca gcaactgtaa ttcgattatt cccgtctttt acaaaccgat
     2341 taatttcaga ctcaaatggt gtaaacccac ctttgtgttg agtaataaac tttccattca
     2401 aaaaaacttt tgcttcatgt gttgcagatc caaaacgaag aactaatctt tcttctaata
     2461 atcttttagg aagagtcact tctttttcat accacaccca tccaacatga tttctaatgt
     2521 tctctgtttc aaataaatca ttataagaag agggaactga aatcggataa ctctcttttt
     2581 catttattgg ttctaaagca agttcttcct gtaagtcatc atttgtatct aatttaaatc
     2641 tccagagacc gcttaagtca attacttgcc tcgtctctgt cataatggga tacaacatat
     2701 aaaccactcc tatctaaata ttaaataaat gtatccttta aaccacaata agcatctatt
     2761 ggacttactc ccttaaattc agaaagaccc attagtttgt ccaccaatat ttctaaagtt
     2821 tcttgttttc catcataagt attaatataa gtctttactt gaggtacatc agccaaatga
     2881 aacggacact gtacagatac aaaaattgtt ggaatttcat gaatgtaaaa cggtatatcc
     2941 ggagttcctt tacttgctgg ccagacaatt cgttgaactg tgcctggatt aacattagct
     3001 atattaatga tcaagtcata ttttttagtt aaatttttaa ttggtctctt agcagcatat
     3061 attccagcta tatttttaat tttttcttct tgcgataatc ctttcaattc ttcttcaggt
     3121 gaaatgtatt tttcaacttc aaatcctttt gctgacaaaa tctgtttcaa taaatcaatg
     3181 gcttgaggtc tatttccagc aactacttca ccaaacccac cttttacccc atgtacatct
     3241 actagtaaaa tccttttata tttttctggt acaataggcc aaatatcttg tttatcttta
     3301 actaatgtaa ttgcttggtc agccacctct ttaaaaattg atttattttc aattaaacca
     3361 attttttcca gagcttcttg cttaggtaat aaaatttgtt cttttgcttt tttgtgtaat
     3421 cccaatttag cttttaaccc taaaattctt tttaaggcat ctaataaccg atcatcagtg
     3481 ataattcctt tacgatagcc ttccatcata taattaaagt cttcatctgg atcattaaaa
     3541 aatagaaata gatcacatcc agctgcaata gaagtgggca ataattctga tcgtttcatt
     3601 gccccagtca ttcctaacat atgactagca tcagtaacta ctaatccatt aaatccgagt
     3661 tttcctctaa gtaagtcagt gattaattct tttgataaag ttgcaggaag taaatcttca
     3721 tctttcagat caggattaaa atgttttgaa taagctggca tatggatatg acctgccata
     3781 attgaaggta atccagcttc tattaagcct gagtatactt ttccaaaaga attatcccat
     3841 tcttccgtac ctaaactatt aatactaaaa gatagatgct gatctcgttc gtcaataccg
     3901 tcaccaggga aatgtttagc tgcaggcaaa attccactct cctgaatacc tttcatataa
     3961 gctaaactaa gttctaaaac tttatcaggg ttttgtccaa aagatcgact agaaatgata
     4021 ggatttctcc aatttctatt aatatctaca ataggagcaa aactccagtt acatccaata
     4081 gccgatgctt caatcccaga aactctccct aattcataag catatttcgc atcatttgtt
     4141 gcaccaattt ttacttctac accaacttca gtaccatctg tacaagctcc gttaccacct
     4201 gcttccgtat tagcagcaat taatagagga attttacttt ctgtttgtaa gatatagttt
     4261 tgatcatata cctcatcagc agttcctgga ttatatcgta cagctccaat atggtactga
     4321 ttgaccatat tactcaagta atttttatca cgactagatc ccatattaac aaacaattga
     4381 cctatttttt cttccaaagt catagaatca atcgtctctt caatccaaga aatagcatct
     4441 tcatctaaat tatatggttt ttttcgtaaa tctactttta tagtcatttt ctttctcctc
     4501 tacttattag taagattttt ccaaaaagta tctatatatt tttgttcaat atctacataa
     4561 tctcccgata aacatttaaa taaaacatca gttcttaagt tttcccatga ttctttttct
     4621 ttattgacca aaataggata aaagaaaact ttatttttat ttgcagcttc tagatcacca
     4681 ggagaatcac caatcattaa tatcttatcc aaatcacaac cattttcaat tacacttcga
     4741 ataatatctt ctttttttcc tttatcttga caacacagtt catcaactaa tgataataat
     4801 tcatgtcttt cccattcttc ttgaacagct tccttattag ctgagcttac aataattatc
     4861 ttcccatatt ttttcagagc ttttatagta tctctagcac ttaaaaaggg tttatctaat
     4921 ccttttgctt gactaatctg ttgattgact tcttccgacc attttaaagc ttttaataaa
     4981 tcctctgact gagaatattc tatttccctc ttcaaagatg gattagacaa ttcttttgtt
     5041 agagataccc agtgctttaa tcgattaatc gatgttacat catttttttc taatgtttta
     5101 actaaaccta aaaatctgtt tattccacga gtctctgaat ataaattaat attattccaa
     5161 tcctgtagga atttttctct atgatgaaca tcaaaaatct ctgcagcaat tggtccgaaa
     5221 aagagattgt gtttataagt catcgtatcc ataacacagc catctgaatc aatacaaaat
     5281 atccaattat tcatatttct ctcctattta tgtttttgtt ttaaaagctc gcaattttct
     5341 aagactttat ctttagataa tggataaagg aaaaataaaa ttaatcctac aatcagaaaa
     5401 caaattgatg gaattaatgt tgaagcgtta tataactttt ctaaaacatc tcccgtttgt
     5461 acatttgcta aagagtcata gcctataata gaaagagtcc aacctccgac gcctccagct
     5521 agagcttgac ctatttttct agcaaaagaa tataccgcat atacggtccc atcttctctt
     5581 tgatcagact ttacttcttg atcatcaata acatcagtaa tatttgccca aataacagta
     5641 ttaaacacac ccaaacccat atagccaacc gaactaagaa ttaagaatac atacatatta
     5701 ctagctttta tcaaaaataa gatcaaatat actattgctg aaaagaacat tcctactgat
     5761 gccagttctt tttttccata ttttttactt aaaaacaaag ttaaaggcga aacaactatt
     5821 aaaaccaaaa taggattgat aaaattcatt aaaactattc cctgtgcatc attatagtaa
     5881 tcaggaaata cataattatt catactcata attaataatt gagcaaggag taagaaaata
     5941 gccgcaccaa ttatagaaag taatgcccta tttttaacta acccttttaa agattttaac
     6001 aatgaaactt tgtattgaga ttggtcaatt cttactcgtt cagtagttag tttataacat
     6061 attaaataac aaataagtgc acatatcgaa aatattcccg caacaattgt aaaagttgat
     6121 ccaccacgaa ctatttgagc gcctgaatca tctcttgtat aaataaataa tggtgttaat
     6181 acaccaatga ccaaacttgc taaagtagct ccaacatttc ggaatgtcga tagcattgtt
     6241 ctttcttctg taatagaagt aatagctgat gccattgatc cataaggaat atttatagat
     6301 gt
//
LOCUS       DAZBSA010000127         6173 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000127 DAZBSA010000000
VERSION     DAZBSA010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.128"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            725..811
                     /locus_tag="ACHGMT_002542"
     CDS             725..811
                     /locus_tag="ACHGMT_002542"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFN6836248.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="ACHGMT_002543"
     CDS             complement(932..1621)
                     /locus_tag="ACHGMT_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836249.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1866..2780)
                     /locus_tag="ACHGMT_002544"
     CDS             complement(1866..2780)
                     /locus_tag="ACHGMT_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFN6836250.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="ACHGMT_002545"
     CDS             complement(2762..3142)
                     /locus_tag="ACHGMT_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFN6836251.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="ACHGMT_002546"
     CDS             complement(3592..3879)
                     /locus_tag="ACHGMT_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFN6836252.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="ACHGMT_002547"
     CDS             complement(3899..4123)
                     /locus_tag="ACHGMT_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFN6836253.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="ACHGMT_002548"
     CDS             complement(4578..4748)
                     /locus_tag="ACHGMT_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836254.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="ACHGMT_002549"
     CDS             complement(4763..5296)
                     /locus_tag="ACHGMT_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFN6836255.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..>6173)
                     /locus_tag="ACHGMT_002550"
     CDS             complement(5317..>6173)
                     /locus_tag="ACHGMT_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFN6836256.1"
                     /translation="IVSRMREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEK
                     ILSLRFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYS
                     LQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPI
                     RQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALAL
                     ADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLL
                     SDLDNLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAZBSA010000128         6034 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000128 DAZBSA010000000
VERSION     DAZBSA010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.129"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(370..1239)
                     /gene="scrK"
                     /locus_tag="ACHGMT_002551"
     CDS             complement(370..1239)
                     /gene="scrK"
                     /locus_tag="ACHGMT_002551"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFN6836257.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(1259..2380)
                     /locus_tag="ACHGMT_002552"
     CDS             complement(1259..2380)
                     /locus_tag="ACHGMT_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFN6836258.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(2382..2777)
                     /locus_tag="ACHGMT_002553"
     CDS             complement(2382..2777)
                     /locus_tag="ACHGMT_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN6836259.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(2796..3593)
                     /locus_tag="ACHGMT_002554"
     CDS             complement(2796..3593)
                     /locus_tag="ACHGMT_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN6836260.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(3595..4383)
                     /locus_tag="ACHGMT_002555"
     CDS             complement(3595..4383)
                     /locus_tag="ACHGMT_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFN6836261.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(4397..4873)
                     /locus_tag="ACHGMT_002556"
     CDS             complement(4397..4873)
                     /locus_tag="ACHGMT_002556"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN6836262.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(4945..>6034)
                     /locus_tag="ACHGMT_002557"
     CDS             complement(4945..>6034)
                     /locus_tag="ACHGMT_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFN6836263.1"
                     /translation="CSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMPL
                     MTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTRM
                     AIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKRI
                     LEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIEDM
                     ILGMNIRKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDAI
                     YILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFKQ
                     SFSVVEHYYSVDIPIHEVKYVYDYVKRA"
BASE COUNT         1900 a         1278 c          873 g         1983 t
ORIGIN      
        1 ttatttttac tctcacttaa aaaagcacta aaattataag agagggaggt aatggtctga
       61 acaccaagaa ttgttccaac atcgaattgc ttaactaatg agttcaaagc ttaattatgt
      121 ggctcttata tttaccgagc gtggattgcg atctctttag cgaaacaaac atctttcata
      181 aaaaaaacag ccatatccgc tttcttggat atgactattt actactactt tactatcttt
      241 cattcttctt gtctcactta tgataaggcg ctgggtatca tgagtgaatg atattgctga
      301 atacaaatag tccgaccaca ttaagttgat cggacttcat aagcatttta ttgactaata
      361 cttttttgct taagaattga ttctaacatc ttttgctaaa gttaagcatc ctatagttcc
      421 tgcattatct gctaacttag gtgtgacaat gtattcttcg atatttggcg tatcgacata
      481 gccgttaatt agttctaaaa aacgtctttg tactttcttt ttcagatgtt cttgttgcat
      541 gacaccacca ccaaaaatta taatatctgg tgaaagcatc agggtcgtat tacaggcaca
      601 ttgtgcaata taatccgcct cgatttccca gaacggatct tctatagaaa gatcttgtcc
      661 cttggttccc gtacgcatct cgatagcggg tccagatgcc aacccttcga gacaatcatg
      721 atgaaaagga caagtaccag agaaagtatc tcctgaatgt ctcttaacta acgtatgccc
      781 catttctgga tggctaaacc cttcaacaaa aatcccatct tgtatagctc ctccaccaat
      841 tccagttcca acagtgtaat aaacaacact ggataaacct tttccatgac ctgaaacata
      901 ctccccgtaa caagcagcat tcacatcggt tgtccaggca attggaattt cgaaatgctt
      961 attcaacgtt ccaacaaaat caaaattttt ccaggcagtt tttggagtag aggtgatatg
     1021 tccataggtc tttgattcac gatggatatc aatcggtcca aatgacccta ctccaatgct
     1081 ttctaattgt tctttatagt gattaaagaa atcaattacc aaactcattg tttcattcgg
     1141 tgtagttgta ggaaaactca ctctttctaa aacctttaaa tcatcattgc caatggcaca
     1201 aacaaattta gtcccacctg cttctattga gccatacatt tcttactcca ccgtcctttt
     1261 atctaggaat tcgttcttcc ctgtctaata gtttttcgaa aaactcatgt ggttcctttt
     1321 ggtaccaata ggcaacagaa gaaatatcgt cttgacgctc ataaagcttg atgtcatcat
     1381 ttcctagggt ttggaaagta acacgtaaat tttctttgaa actaatgggg tcgggtaaat
     1441 gccagcggta caacccatgc atcggtaaag catcatcgcc aaaaccatga actggatttg
     1501 gattaggttt tccagcacta aaataatccc gggtgtgatc tcttttagat tgaaaaggat
     1561 agcccataaa tagcgtatta aaattttgag catatgccct ttctccaaag ttctttttat
     1621 ggaaagccca agcgccgcca aaataatctt ctgctcctgt agatgttatt gtagggaaat
     1681 cttcatctcc atctatataa aacttaaatt ctccttcccc ccaccagtat ctttccaatg
     1741 cgcacaaagc aaagtaggtt cctacataat acccagtgcc tttgattcca tcaataaggg
     1801 tgtaatcttt tccttttgtc gtgatgggtt cacggttcca tttagcatga aaatacatcg
     1861 catcatccgg tacttcagtc aatgcataat taaatgtata aaaaatgtgc ttaataaact
     1921 caggatgttc attcgttaaa gtaatcttag ctttttttcg aaatggcatc tcaaaatagc
     1981 aattcatccc tgatgttgga ttcactacga ttggtaagga attcacttcg catcttgtcc
     2041 caaacccatt acaaaaaaaa tctcctaatg gactttctac agaaggagtg gattcatcat
     2101 cccaataaat acgcaaaatt aaatcacgta agacgaaact cccagcgatt gttttgtctg
     2161 tcactgtcat ccaaaaatga cgtaaaattc cagggccttc catttctgct aaagtaattg
     2221 tctctccagc atttatcgga acagaaggtg acccttttct cccaattcct agatgactcg
     2281 ctgtcattgc cgctttccct ttttcaccat ttttattttc tggtgaaata gttctacttt
     2341 cctctccctt gtggagccat atattattta aacaattcat tttattcgtc ctctatcctt
     2401 tcttcacttt tttgattatt catatagatg atttgttgtt gtgcctccgc aataattgct
     2461 ccaatatcta aattatcatt tcctaactgc aaagcgattg acattgccaa gggaagattc
     2521 attcctgcaa ttacgtgcac tctgtcagaa atataggtaa aaaacttttg atttacactt
     2581 cctccagcca tgtcagttaa aacaattatt tcgtctgtag tatccactaa ctctttaatt
     2641 tgttcatcta tatcatcaat ttcttcgaca tatgcacata agctactaat ttgattatgc
     2701 atacctgtta aaaactctag tgtctcttta aaaccctcag ctagtattga atggctagca
     2761 atgatcaatc ttctcataat tcaaatcctt ttcctttacg ctaatattcc aaatgcagcc
     2821 ataaccatcg caaaaacaat aacgagtaga atcaaaatgg tcatactgat ttttttctta
     2881 ctgagtagat aatatgctgc agcagtaata attactggaa ttaatgttgg taaaattttg
     2941 tccaacattg gttggatttt catactaact tcaccaaaag tgaatttaag tggcgttgaa
     3001 acactgataa ctgaagcaat taatgatcca actactgtta gccccaatac tgaagcagct
     3061 tcagttaaat aattaatctt ttcaccgtat tcagtaacaa tcttcattcc ctgcttataa
     3121 ccaaattcaa agcctctcat tcggaaaaag aagattacca tgattaaaat gaaccataaa
     3181 aagattccta caggattatt ttctaaagcc atataagccg caattgatcc catgatggtt
     3241 gggatcatcg tcatcaaaag agagtcccca ataccagcaa ttggacccat cgtaccaact
     3301 ttaaagtcat taatcgcatc ttttgcttct aatccttgct tttcctccat tgcaacacaa
     3361 gcacctaaaa taatggctgc cagccaaggc tgtgtattgt aatactgaaa gtgattattt
     3421 aaagcttcga tgtattcatc atcatctttg tataattttc ttaaaatagg tgacaatgcg
     3481 taagtcacag atgcaccttg ctgtgtttga taattaaagg tacaaactga catcaaccaa
     3541 cgatagttcg ctttcctaag atcctttttc gttacttgat atttttcgct cattttattc
     3601 atcctcccca ataaacccaa catcagaagc tcccgcagaa gcagtgattt gtgcatcttt
     3661 ttcagctcta tgatacacat taaatgctaa agcgaatcct atgatcgccg ctccaataat
     3721 aggtaccttc aaaaaagcgg ctaagaaaaa tccaacaagt aaataactaa tgtatcgacc
     3781 tgtaggcata aagcgtaaca acatcgcaat accaacagca ggcaacattt ttccggctaa
     3841 tgttagccca tccataaacc aattaggtaa aacatccaaa acgtaattaa cagcagactg
     3901 tcctaaagta acggcaataa atactggaat tgctgcagat aaaccaaaga aaattggccc
     3961 caaccacaaa acactattca ttttcttaaa ttctcgttta ttcgcatatt cctgtgattt
     4021 gcgaacaaca aaaccattta agattttgac aattacatct agttgcaccc ctaacatacc
     4081 tacaggtagt cctattgcta atccggcttc tactccaagc tggctaccaa atgccgttgc
     4141 aataattgcg cacgttccat agtccggtac agatgagcca ccaaaaccgg caactcctaa
     4201 tgacatcaat tgaactgtac cgccaactat caaacctgtt tttatatcac ccatcaaaat
     4261 tcctgtgaat aatccccaaa aaactgcgta atttacaaag atcatcggaa tcccataata
     4321 atcaacatgt ttaataaatg ctaatatagt cagcataata atttgaaaaa tagtaaaatg
     4381 cattatcctt actcctttat cctaattttg attgaatttc ctgtgcctta tccgccggaa
     4441 caaattgtgt aacaatttct acaccagaat ccaacaattt tttatacgtg ctgatatttt
     4501 catctgatgc ataggctcta gaagatagct gtaatgcatt atctttaaca acagtaccgc
     4561 caacaattag tcggtcaatt ctaataccgt tatctactaa atgaaggatt gtttccggct
     4621 ctttcacgat gacaaatact ttttcctgat cgtatttccc ctttttaaaa ttcgcaatcg
     4681 ctgtttcttc agtgataatt gatacagcca ttccagcagg tctagctaaa cgcatacttg
     4741 atttttggat gtcgttattt gcgacagaat catcgatcac catcactctg tttgctttag
     4801 acattggtgc ccattgcgtg acaataatcc catgcaataa acggttatca actctcgaca
     4861 taacaatact cataaatact tcctccttta tctttcattt tcttacaagc aattaccatg
     4921 ccaaaacaaa aagaacttga taaatcaagc tctttttaca taatcataaa cgtatttaac
     4981 ctcatgaatc gggatgtcga cactataata atgttcaacg acactgaaag attgcttaaa
     5041 atgtgtctga aatttttgaa attcctcact cgtttcattg aaataaattt catcttccaa
     5101 agtattttgt tttactaatc gttcaattaa gcaactaatg tgaacatata atccgaaaca
     5161 cgtattatta tcaaatgaaa ttcctagatc attttgtaag atgtatatag catctgaaac
     5221 ccgttctaat aatttagtcg gatttaatat ggtcaattgc tccattaaat tcgttagact
     5281 aaaattcttc agaatattat tgttcaaaac tttcagttca ttatcattaa agtgtctatt
     5341 caattgattg gagaattttc ttatattcat tcctaaaatc atatcttcaa ttgaaaagaa
     5401 ttctacatct tctagatgtg gatttagtgt tccaacaata aaaagaatat tgttctcttt
     5461 acgaagctcc gataaatagt tttcattcac taaagagtaa tagtcacacg tttctatgac
     5521 aagatcagta tcccttggaa agctatcttc aagtatcctt tttaatttaa acgaagttcc
     5581 taatccgctt gcacaagaac agacaatcat atccttctta atatttgatg attcataaat
     5641 ataatccaca ttttgatttt cttttacttg ttcaagaatc tgtttaagcg gtaaatctaa
     5701 taatatctga tttgcaacat caatagccat tcttgttgaa acattgttta gtagtccgaa
     5761 ttttttttga gcgtctagtt ccaaatttag ataaattttc tccagtgaac ccatatcaac
     5821 aagtaaaata atttcttcat atccggtaca ctcttgtaag tatctattaa ttttgtctgc
     5881 aatctcacta cttgaagtca ttagtggcat atcaatggcg tcgtatatat atctctcaac
     5941 gatcctatta gcagtacttg ctatacaggt tgccgttaga tctccatgag ccaaaatcag
     6001 tgctaaacgt ttttgctgaa attctttact acac
//
LOCUS       DAZBSA010000129         5932 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000129 DAZBSA010000000
VERSION     DAZBSA010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.130"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(158..616)
                     /locus_tag="ACHGMT_002558"
     CDS             complement(158..616)
                     /locus_tag="ACHGMT_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFN6836264.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(629..1960)
                     /locus_tag="ACHGMT_002559"
     CDS             complement(629..1960)
                     /locus_tag="ACHGMT_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFN6836265.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1971..3413)
                     /locus_tag="ACHGMT_002560"
     CDS             complement(1971..3413)
                     /locus_tag="ACHGMT_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN6836266.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3400..4164)
                     /locus_tag="ACHGMT_002561"
     CDS             complement(3400..4164)
                     /locus_tag="ACHGMT_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFN6836267.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4268..5143)
                     /locus_tag="ACHGMT_002562"
     CDS             complement(4268..5143)
                     /locus_tag="ACHGMT_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFN6836268.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5398..5482)
                     /locus_tag="ACHGMT_002563"
     tRNA            complement(5398..5482)
                     /locus_tag="ACHGMT_002563"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5446..5448),aa:Leu,seq:caa)
     gene            complement(5500..5571)
                     /locus_tag="ACHGMT_002564"
     tRNA            complement(5500..5571)
                     /locus_tag="ACHGMT_002564"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5537..5539),aa:Gln,seq:ttg)
     gene            complement(5581..5653)
                     /locus_tag="ACHGMT_002565"
     tRNA            complement(5581..5653)
                     /locus_tag="ACHGMT_002565"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5618..5620),aa:His,seq:gtg)
     gene            complement(5715..5799)
                     /locus_tag="ACHGMT_002566"
     tRNA            complement(5715..5799)
                     /locus_tag="ACHGMT_002566"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5763..5765),aa:Leu,seq:caa)
     gene            complement(5820..5890)
                     /locus_tag="ACHGMT_002567"
     tRNA            complement(5820..5890)
                     /locus_tag="ACHGMT_002567"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5856..5858),aa:Cys,seq:gca)
BASE COUNT         1820 a         1260 c          902 g         1950 t
ORIGIN      
        1 cctgaaaata agttttctaa atcataggag caacaaaaaa attcgataat taacaaaaat
       61 caaaatgatt aaatctagtg attgaaacat atcatttcaa aaaaattttg taaattcacc
      121 cataaatatc accctattta acttttttca tcgtacacta aaattcaaaa caactatctt
      181 taatctttac ttcttggttt tgttttaata aatttccttc aaaaatgcct ggagaaacta
      241 caatgatcac cgtatctttt tgtgcttgct ccaacttctt caaatctata atagctaaca
      301 gtgtcccttc ttttacctcc tggttacttt caacccgtat ctgaaacgga cttccttcga
      361 gttcaactgt atctaaaccc atatggatca gtatttttct gccagattca ctttcgatcg
      421 ttatggcatg tcgggtagga aatatattat cgattattcc tttaactggc gcatggatat
      481 atccatcatg attcgttata gcgtatccaa ctcctatcat ttttgaagaa aagacctcgt
      541 cttttactgc tgttaatgga ataatttgac cactagctac agcttttaga atgtttcttg
      601 ttttaaagaa atgcactttt tatcacctct actttaatgc tggccaatat tttttgttta
      661 ctttgatcat ttcatctagt attttttttg cgactctggc atttggtacg gtttgattca
      721 atgtaaatgc ttgtaaagct ttttgataag aaccttcaaa gtaagcatct actagtaact
      781 tctctactgc aacttgtgcc tccatcaatc ctttatgaaa atcactgatt ggttttctta
      841 gtgtaattgg ctccacacca gttgcaccaa catatgccgg tatttcaaca actgcatctg
      901 accgtagatt cggaatcgcc ccttgattag gaacgattag cataaagcga cgacgatcat
      961 cattcaacaa ggaaatagct atatcgacga tatattgtcc atgctcccca aaattaaagt
     1021 tcaatacttc ttccacaata ccgcggcgta ttttatcagc catttctttg gtatttttct
     1081 ctcgtccatc catgaccata ttagcacgtg tatactcttt gtcagtatgc tcaacgacca
     1141 tatcaggata taagtaatac tctaagtaat tattcgggat attacttgga aaatttttcg
     1201 tgatgactga catcatttga aaggtttttt gccaagtttt atctcctata ttaaaatcgg
     1261 cgacttgcat ttcttttgtt attagttttt caataatctc tggcataatt tctcgttgca
     1321 attctttatc ataaatcgat gtataccagc caaagtggtt taaaccataa tacgtaacga
     1381 tccaattctt acgatcataa ccgtaattca ctgcaatcgt ttcttcaatt gaaatagtca
     1441 tatcacaggc attaatcatt tttacattcg gatatttacg acgaacagct tcagaaacga
     1501 tcgactctgg atttgtataa ttcaaaatcc atgctttggg ggcatagtct tgtatatgat
     1561 cgacaatttc caataaacct ttgattgagc gcatcccata agcaaatcct ccgagtccac
     1621 acgtttcttg tcctactaat ccatgtttaa gtggaatctt ttcgtccttt tctcgcatct
     1681 ccaatcctcc aacacgaatt tgtgagaaaa caaaatcgca tcctgtaaaa gcaagctttg
     1741 gatcatccgt tgcacgaatg cgaatatttg aaaaaccttt tcttttcagc ataaatttaa
     1801 caatcaagta catatccata tttctttctt catcaatatc atataaacgt atttcagata
     1861 atggcaaccg tttttcattg ttcaaaatgg cttgaacgat tcctggtgta tacgtactcc
     1921 cgcctccagc tatagtgata atctgttttt tcatcatcat cttttccttt ctattcttct
     1981 aataatttat tgaattctgc cataacataa gtcacttcta agccgatgat aatttgataa
     2041 tttttttcag ttggtttaat gatcccgctt atccctaatt gcttgattcg ctgttcctcg
     2101 accatggtgg gatccactac ctctacccgt aggcgtgtat tacaactata gacatccagg
     2161 atattggctt ttcctccaaa cgcttgtagc atttttgttg ccatatattc ataattttta
     2221 cttgctaacc ctaactctgt ttcttctaag gttacttcat tgccaaactc tgccttagct
     2281 tcacgtccta atgtctgaat gtttctctta ttgatcaaat aataaaaagt aacgtaatac
     2341 aaggcaaaaa agaaaattcc gattggaaga atcaaaatag cattggtagc gattcgaaaa
     2401 ttgatgagat aatcaacaat acatgctccg aatgtaaaac caattcgtat attgaataag
     2461 taacagacaa tccctgctaa accggcaaaa agtgcatgga tcacgtacaa cataggagaa
     2521 agaaacatga aactaaattc aacaggctca gtgatatttg caataaatga agttcctgca
     2581 ccgcttccca tgattccttt gaccatctct ttattttctt tataagctgc tttatagata
     2641 gctagacata tagctggtat accaaacatc attactacaa aaaaaactga taaaaacagt
     2701 ccagcctgag gatctccgtt gataaagcgt gtcatctctc ctctgaaaac ttccttgcta
     2761 ggagatgtat agcttcctaa atcgtaataa atatacgtat tcaacacatg gtgcagtcca
     2821 aaggggatca gcaaacgatt caaaaacata aatatcccca cacctaatgc tcccatatca
     2881 acgagcagtt ttgcaaaatt atcaatccca atttggatga gcggccagat aaagcccaaa
     2941 aaaacagctg cgattgtttg tagaatcatc accatagtca atgggaattt ttcgcccgca
     3001 aaataagaaa aaactactgg taatttttgt tctttaaagc gattatacgt ccatgccgct
     3061 actaaaccgg aaataattcc accaaaaatc cccatattca cttctgggtc cataatttct
     3121 aagccttttt tcaaggtagc aatcgctaaa aaaccagtaa gtgcaggtat tcctttgtcc
     3181 ttatttttga caaaaccgat tgcaactcct atagcaaata agatatccaa gtttccaaaa
     3241 acggcatttc cagctgcacc aattactggg atgttcagca tatcgtcagc tgataaacgc
     3301 atgatcaatc ccgctaatgg catcgctgca actggaagta gcatcgctct tccaagcttc
     3361 tgcacatttg atttaacatt catttttatt cctcctctat taaatatcct catatgttac
     3421 taactcaatc aaaggatcat ttttcacttt ttgagcaaat gctgaatgaa ttaaaaattc
     3481 gacttggtgg gtacgaggat acacaaaact tgaaccattt aacaattcct gatccaaata
     3541 cgctgcgtga cacatgactt cgatcgtttt atcttctctc aatatcatga gtaaacttgc
     3601 caaatattct tccacactcg tattgatttg tcgttttttt tcatcagttt ctcctacgac
     3661 atcgaaacaa ggctcaaaat aatcgacatg acgaacttca tcttttcgtt caaagttgct
     3721 ccgaaccggc aaatcatatt tttttgctaa tgtaacaact acctcctgat tttcatccaa
     3781 agtatgagta tgatgatgac tgtctaaatg agttggtatg atccccgcat gataaatttt
     3841 ttgaatctga gcattccatt cctgatacag ttgatctttg tcaatgttaa atggatgctg
     3901 ataataagat aaggatctga actctccgct ttcgataaga gtatcgacag ttttgaggag
     3961 tggtttgcca cacgtcaatg ttaaatggac tcctacacct agcctaggca tttcctttct
     4021 tagtttcaca gcatgctcga aacccggcat atttgccatc aatgtagttg atgtcaaaat
     4081 tcctcgctga taagcatcca taatcccata attcacacca tggctatagc caaaatcatc
     4141 cgaattaaaa atcacttttc ccatttcatt ctccttcttt tttctactaa aataggcgca
     4201 accaaataat catagtccat ttactatgtg tcatttggtt gcgcctatag atagttacgc
     4261 ccttatttta atagattctt gctagatgaa ttgccaaata gatgatttca ttttcttgaa
     4321 tggcaagttg aaattgctgt tcgatgatat ctttaatctt tagtgctgta caatacgctt
     4381 tgctatgttt aaatgtttca gtaaaaaaac caatattttc tactggcgtt tcttcctgat
     4441 aaatcaagcg tttaaccagg aatttgatat gaaccattaa gcgatgataa gtcagtgatt
     4501 ctttatcata aacgtttgaa taggattttt ctagatggga caaaatcgtg gtcgtcattt
     4561 ctactatttt taagctcttt aaactattag gctctcctag ctcagcatta atgatatgca
     4621 tagtgatgaa tcctacttca tttgacccta actcggtttg atatcgatga ttgattcgct
     4681 cgaccacttc tactgctata tcatattcat ttggataaag gaatctcatt tctggcaaca
     4741 taattgctgt gacatctcta tcttctcttt ttcgttgaat agcaaaataa atatgatctg
     4801 tcaatgtaat atagatattt gaatctaatg gagaggagat cctagctgtt atcagttcaa
     4861 tcacttcttc cgcaaaatcc ataaattcga ttggaatctg cgagactatc tcactgaatc
     4921 gtttgatctt ttctttagaa tctaatacaa ataatttgtc agcatcctca acatcaaatg
     4981 gcttactcac tttagcagaa aagccaatcc ctttccccat cgcaattttt tctattccat
     5041 cgtcatcgat caatacagca ttatgattca gctttttcat cacatacacc gttaattcac
     5101 ctcctctata tgataaaaca aaacaacttg cagactcctt catacatatt tttgtctcca
     5161 aacaattttt aaagacaaaa aacgacctaa gaaagaaccc tttcttaggt cgtgcctaat
     5221 tgaatagtag caccctacat aatttacaaa aatagtttaa cacataataa aagcgttatc
     5281 aatatctttt ttactcgttt tatgataata cttgtatcga attgatttgg aggtaattac
     5341 ataaaaacaa aaaaaatata gcaactctct ataagaaagt tgctatattg ataattatac
     5401 cggcggccgg ggtcgaaccg gcacgtcctc aacggacact ggattttgag tccagcgcgt
     5461 ctgccaattc cgccacgccg gcaaatttaa aaataataac tggggtagct ggattcgaac
     5521 caacgcatga gggagtcaaa gtcccttgcc ttaccgcttg gctatacccc aagaataaag
     5581 ggcgaatgat gggaatcgaa cccacgaatg ccggagccac aatccggtgc gttaaccact
     5641 tcgccacatt cgccatcata aaagatgtta tttaatttct caataaaaaa ctaagatgaa
     5701 gacatcttag tcaataccgg cggccggggt cgaaccggca cgccctcaac gggcactgga
     5761 ttttgagtcc agcgcgtctg ccaattccgc cacgccggca agttgattta ttagtactaa
     5821 ggcggtaacc ggatttgaac cggtgatgaa ggttttgcag acctctgcct taccacttgg
     5881 ctataccgcc attatactat tatcaatcta taaaataaag aataaattat ag
//
LOCUS       DAZBSA010000130         5769 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000130 DAZBSA010000000
VERSION     DAZBSA010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.131"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..300)
                     /locus_tag="ACHGMT_002568"
     CDS             complement(<1..300)
                     /locus_tag="ACHGMT_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFN6836269.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
     gene            393..742
                     /locus_tag="ACHGMT_002569"
                     /pseudo
     CDS             393..742
                     /locus_tag="ACHGMT_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1014..1304)
                     /locus_tag="ACHGMT_002570"
     CDS             complement(1014..1304)
                     /locus_tag="ACHGMT_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836270.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1706..1942
                     /locus_tag="ACHGMT_002571"
     CDS             1706..1942
                     /locus_tag="ACHGMT_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836271.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1949..3901
                     /locus_tag="ACHGMT_002572"
     CDS             1949..3901
                     /locus_tag="ACHGMT_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFN6836272.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACHGMT_002573"
     CDS             complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACHGMT_002573"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFN6836273.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4797..5489)
                     /locus_tag="ACHGMT_002574"
     CDS             complement(4797..5489)
                     /locus_tag="ACHGMT_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFN6836274.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSWKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1893 a          997 c          917 g         1962 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattta agtagttcag ttttggagta caaaaagtga
     1501 aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt
     1561 aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg
     1621 cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac
     1681 taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa
     1741 gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata
     1801 tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa
     1861 gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg
     1921 tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga
     1981 agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa
     2041 acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt
     2101 gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa
     2161 tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc
     2221 aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa
     2281 tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg
     2341 gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc
     2401 agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc
     2461 gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc
     2521 gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca
     2581 aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg
     2641 tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta
     2701 taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt
     2761 taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa
     2821 aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt
     2881 tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa
     2941 tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac
     3001 ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa
     3061 cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag
     3121 atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat
     3181 taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag
     3241 aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat
     3301 ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa
     3361 agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt
     3421 ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag
     3481 taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac
     3541 taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa
     3601 cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca
     3661 gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa
     3721 gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag
     3781 aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc
     3841 tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg
     3901 acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa
     3961 ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata
     4021 cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt
     4081 cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt
     4141 ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc
     4201 ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa
     4261 ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat
     4321 tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata
     4381 ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca ctctattctt
     4441 atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa ctaatttacc
     4501 atctaatttc ttatttctat caaaaaacct tttctcatta tcctgcccgt tagttgaata
     4561 agcaattttt tctataagtt atattatcat aataccacaa aatcacaaat tttcattcaa
     4621 ttatcatttt caattgcatt ttataaatca ttttgtatgt aatttggtat gtaagttaaa
     4681 atgaaaaaag tagtccattt tgaagtacat tttgtcattt tgtactccaa aactgaacta
     4741 cttagtatag attactgttg cttctttact aatttaggtt tacttgttaa gaaagattat
     4801 ttacgcaaat gttcttcaac aatttgctga gatttatcag ctgtatcttc cacttcatct
     4861 tttgcttttt ctaacttttg aatatattgt ttccagcttg agttttcagc agttgcttcg
     4921 tctaatttct tttgtgtatc tgttaactga gtttctacat ttgttttttc ttgtgtcagt
     4981 gttgcgattt gttgattcaa accatttaca gtattttgta attctgaaag ttttgcttgt
     5041 gcttcttgta gtcttgctgt ccaatctgca ttcagatgat tgatttcatc atttttttgg
     5101 ttgatctcat ctagtttgac attgatttca ttttgtttgc tttgtagttg tgcatccttg
     5161 ctgactaatt gactttctaa gttttgtttt tctgctgccg attgattttt ttggttttct
     5221 aattcattta cttttgtttg gtattcttgg acatctgcct ttgaatcttg caatgtctgt
     5281 tctttttgtg ctagtcgagc ggtaagatct gtaatcgctt gctctttatc tgtcaatgta
     5341 tgatcaagct gtgtcagatt ctcattgatt gtatcaatat ctgtctctcc accccacaaa
     5401 ataatttcat ttgcgtaccg cgtgcctgtg tagccaatta gacatagaaa tgcaacgaca
     5461 atcccaccag taataaattt ctttttcatt tgcatagctc cttaatccaa tacttttaca
     5521 tgctaaaagt aagtgatctc aaacacaatg tcaagcgttt ttaaattaca tatgtaattt
     5581 taatataaat actatcaaat actcatttaa tcctcctttt ttatctattt aaaaaatctt
     5641 ttttatagag aaaaatgcag gttaatactt tcttaaataa atatttatgg tgaacaaaaa
     5701 caaaaaagca agttttatta aacgtaaccc ttttcatttc atagattact gtatttatta
     5761 caaaaaacg
//
LOCUS       DAZBSA010000131         5727 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000131 DAZBSA010000000
VERSION     DAZBSA010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.132"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            196..921
                     /locus_tag="ACHGMT_002575"
     CDS             196..921
                     /locus_tag="ACHGMT_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836275.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGFSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            911..1420
                     /locus_tag="ACHGMT_002576"
     CDS             911..1420
                     /locus_tag="ACHGMT_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836276.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1490..2938)
                     /locus_tag="ACHGMT_002577"
     CDS             complement(1490..2938)
                     /locus_tag="ACHGMT_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN6836277.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2938..3654)
                     /locus_tag="ACHGMT_002578"
     CDS             complement(2938..3654)
                     /locus_tag="ACHGMT_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFN6836278.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3635..3985)
                     /locus_tag="ACHGMT_002579"
     CDS             complement(3635..3985)
                     /locus_tag="ACHGMT_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFN6836279.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4129..4902
                     /locus_tag="ACHGMT_002580"
     CDS             4129..4902
                     /locus_tag="ACHGMT_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN6836280.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5073..5439
                     /gene="ssrA"
                     /locus_tag="ACHGMT_002581"
     tmRNA           5073..5439
                     /gene="ssrA"
                     /locus_tag="ACHGMT_002581"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1662 a         1123 c          994 g         1948 t
ORIGIN      
        1 aaaattattg tcgctgctcc tcttttcgaa taaacagtgt tcaaaagctt gcatctacgg
       61 taatagaata gggcagcttc ttcacaacct cttgcttttt acaaaatatt ttctggtttt
      121 aattcgtgat ttcactaaag gaatttcata ctttattcat attcattttc tatacttttt
      181 atagaggtga aaagtatgaa aataatccgc tatgctactg ctcgattaat ttattacaaa
      241 aagcagacaa tactctattg tttattttca agcttttctg cttttttatt gatagcatgc
      301 ctgactcttt accattcgca atcggcttta gccaatcaag tccaagatcg gctttcattt
      361 attggcgctt ctgcatctac agagcttctt ccggctttag aattagctag ccgattttat
      421 ataaaaggca tattcagttt attttttcta tttgccttcc tttttatctt tttcttttac
      481 tattcagtca ggcaaaacaa gcaggaattg atcaattggc gcctgactgg tttttctaaa
      541 accaaattat tcctttttat tttctggcag ctgttctttc ctctctttct ttgttgtatc
      601 ttggttttat tgtggatcgt cagcttccag tcagtttatg aagcgcttct tcaaaggatc
      661 aattttcttg tcttaaagac tactgaactg ccagatatcc gtgatatctt gacttcggta
      721 agcggactta caattccgat ggatcaacag acttttttta ccatcgattt ccaaaatgat
      781 ctttttttca cagatatact taagggattt gcccaaactc ttattgggct cagtggatgc
      841 tgtctcattc ttagtctatt ccctttcact ttttttatcc atcatttgca aagtaggagg
      901 cctggacgta atgaaacctg aatatttgat tcatgctgaa cacttgaccg ttattttgtg
      961 tcctctcgaa aaacagcata aaatattgga gcacttgaaa aaagaggttc ttgttgcaaa
     1021 actggataat tttgcgatca tccaaaaaaa ttggccgatg tttccttatc tgaatcttaa
     1081 agaccatgtc ctgttggatg taccggaaaa agaaatcaaa caagatcgtt tggcctatca
     1141 agaaaaactt gatatctctc cttccttgct taattgtgcg gcagctgagt tgacgtcttt
     1201 tgaaaaagta aagctgcaat tacttcatgc cttgctttcc aagcgaaaca atattgttat
     1261 tgaagataca tttgatgaac ttagtgtatt ggagatccaa gagctactcc acttgctttc
     1321 ttatttagct catgaagaaa atcaaggtat cttactcttt actcatgatg ctactattgc
     1381 tcaaagtcct tatattgatc gtttagagcc cgcgggctaa atcaatcatt ttatagtaaa
     1441 aacaagcggt atagaagatc gattcatgat cttttatacc gcttgttttt tactcttttt
     1501 tgatttctac gtcaggcaaa cgaacagcga attttgttcc ttttcctagt tcgctttcta
     1561 cttctaattt tcctttatgc aagcgtacaa gttgctgcac gattggtaaa cctaatcctg
     1621 attcaccata ttttgtattt tttcttgaag gatctacttt ataataacga tcccagatac
     1681 ttttcatttg ttgttcagac attccaattc ccgtatcttc aatcgtgatg atcgtttcaa
     1741 gatagccttt ttctaaagta atcgtgattt ggccgttttc agtaaactgt atcgcgtttt
     1801 gaataatatt gaccatgatt tggacaaatc ggtcataatc agcgtaaaca tcaatatcat
     1861 caacggcttt caatgtcaat gtatctcctg ctgcttctgc tttagcttct aactgtgcaa
     1921 caatattttt caatgtttct gttccattga attttttaat gaccatactg atttgattcg
     1981 tacggatttt ttcataatcc aagttttcat tcaccaaacg gatcagacgc tctgtttcat
     2041 ttttcatcaa tttaatcgcg ttttctcgct gattttcagg gattgcattg tattctaacc
     2101 cttccagaag tccgttgatt gtcgtaagag gtgttctcat ctcgtgagaa gcatctgcca
     2161 taaattgtct tcgacgattt tcttgttctt caatttctgc ttgagattct ttcaatgaat
     2221 tcgtcatttt attgaaatca tctgctaact catcaaattc atctttgtca tgtacgggga
     2281 gctgtacatc aaaattacca ttcgtcactt ctttcgtcgc attccttaac cgattgatac
     2341 gcttaacttg gaatgctgca aaagcatagc tggcaataac tgcaacgata ctggaaaaaa
     2401 taaatccttt gaacaggttc agtgtaattg gacgaacgct gttatcgata tttcgcgcag
     2461 gttgactaac tacaagtgct ccatagaatt catgattcaa attaaatggc acaagtgcgt
     2521 aagaggtcgc ctgtgcttgt cctaaaatat ttttattcga tgtgaatttt tcttgacgtc
     2581 cattcctcag atttttccat tgatcagctg ttaaggtaaa attcagtaaa gctgtattag
     2641 tcggataaat gacacgttcg tttttatcga taaatataaa gttcacattt tgttctgtca
     2701 gtacttgctc tgtcagcagc aatgcatttt gaaacgcctg atcttgacta aactgcggga
     2761 gcgtatcggc ataggtttgc gttgtctttt caactgattc agcatatcca aacagttgct
     2821 ggtaattgtt ttcttcaatc gtttgttttg tcagttgtgt aaaagaaact ccaacgatca
     2881 ataagatcaa cgcgaccatt ccgcaaaaag ctaagagttg ctgatacaaa taacgcatta
     2941 ggcaacacca gaatcatcga atttgtagcc gactccccaa acagtttgga tgacttgtgg
     3001 tccgaccttt tcgatttttt gtcgtaattt cttgatatgg gcgtccactg tccgttcatc
     3061 accaaagtac tgataatccc agactaattc aagcaactgc tcccgagaga atacttgacg
     3121 tggttttttt gctaaagtaa acaatagatc gaactcttta ggcgtcagtc cttcaattgg
     3181 ccggttgtcc agatacgctt ctcgtgtttt cgtattcatc ttgaaatgac tagtctcgat
     3241 atcaaaatga tcatctgaaa ctgctgcttc ttctcttgct tctgccaact cactacggcg
     3301 atgcaaagct ttcattcgag caataagcgt cagaggactg aaaggttttg tgacataatc
     3361 atctgccccc atctctaagc cgatcacctg atcgctttca gaatctctag ctgtcagcat
     3421 gataatcggc acagtacttg aaactttgcg gatctctctt cctactagca taccgtccag
     3481 tgtgggcaaa ttcaggtcta gtgtaatcat atcccaatgt tgtgggtttt ctaaaaaggc
     3541 atcgagccct tcttttccat catacttgaa agttgcttcc caaccttcat ttaagaaaaa
     3601 catctgcatc atttcagaaa cagattgatt atcttcaatc attaaaatat tcattatcgt
     3661 tccctccagt ttccaggtca tatttctgta atttcttttc tgtcataata aaaataggca
     3721 taattggaaa tactagcaga agcatttcaa tcagaaaata attcgtatgc ccagatgtct
     3781 ttaaataata gccgatcaat gccatcagta agcccataag aaaaaaccag tttccgcttg
     3841 tcttttgagc cagccgccaa tgagcgtctg tttttgcggc aagaggcgaa tgatacccat
     3901 aaaaaggcag atttcctttt gagggcagaa tccgaaaaag actgccaatt attaagaata
     3961 agataccaat actgaaaaaa atcattcttt ccctcctaaa ttctcttctt atagtatact
     4021 ctaaattgtt gactgaacca atatgttcag gaattcttca cgaaaggttt gtcttttgtt
     4081 cattctttgt taagataaac ttattccaat aaaaaaggag ttttatttgt gtcaaattat
     4141 cgtgcaatca ctttctttga tcttgatgga acattactag atgcgaaatc aaaaatcaca
     4201 cccgaagtgg caaaagctat gaacgcatta aaagaaaaca atgttctccc cgtcatcgct
     4261 acaggacgta cagaagcaga aatccactct atcatggaag atgcgggcat tacttctgcg
     4321 gtcaccatga atggttcttt cattcgagta gacggcgaag aaatctattc tcaaacattt
     4381 tcttcagaag aatgtcaaaa aatgtatgat catgtacagt tgaacggtca tgaactttct
     4441 tactataacg atcagaaaat ctggtgtacc ggtcatagcc aaacaatgat caatgcttac
     4501 gacttcattc attcagatgt tccggaaatt gatcctttag gctttaaaga aaacacagta
     4561 aatatgttgc tgatcttatc tcaatcaggt gacgaatact atcacgaaca ttttcctgag
     4621 ctaacttttt atcggaatgg tccttactcg atcgatacag tgaagaaagg aatctcaaaa
     4681 ggggcaggtg tcaaacgtct gcaaaaagaa cttcagctgg aaaaagttcc tacttttgga
     4741 tttggcgatg gtccgaatga ttttgccttg ttggaagctt gcgacaacaa aatcgctatg
     4801 gacaacgcct atgacggatt aaaagaactt tctaccttta tcacaaagaa aaatacagaa
     4861 ggcggaattg tccacgcatt gaagtatttt gatctgattt aagatctttt tttgaagcta
     4921 cctcttttct taagaaaatc gcttattttt tcttaagaaa ggggcagtta ttatataaat
     4981 aatgggatat gattcgcttt tttattgaat ttatcccaat atatgctata ctaattttgt
     5041 atgaaaaata cacgtaaccc ttgttagcta acggggacgt tacggattcg acaggcacag
     5101 tcgagcttga attgcgtttc gtaggttacg tctacgtaaa aacgttacag ttaaatataa
     5161 ctgctaaaaa cgaaaacaac tcttacgctt tagctgccta aaaacagtta gcgtagatcc
     5221 tctcggcatc gcccatgtgc tcgagtaagg gtcctaactt tagtgggata cgtttcaact
     5281 ttccgtctgt aagttgaaaa agagaacatc agactagcga tacagaatgc ctgtcactcg
     5341 gcaagctgta aagtgaatcc ttaaatgagt tgactatgaa cgtagatttt taagtggcga
     5401 tgtgtttgga cgcgggttcg actcccgccg tctccatata tgtatggttg agacagataa
     5461 acatgtatga aaaacgttgt taaatcaacg tttttttatt ttggtcaagg agagaaaagt
     5521 agagaaatac aaaatttttt gcattgattt tgcattcaaa aaaacattca aacatttaga
     5581 aatccccaat acgtattcag gaaaatctat tttaaggttt acaaaaagaa cttttgttcg
     5641 tacactcttt ttgaggtgaa cttttatgct gatggaagga aaaacacaat tatggtttaa
     5701 gcatgttcct tcaaataagt tttttta
//
LOCUS       DAZBSA010000132         5532 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000132 DAZBSA010000000
VERSION     DAZBSA010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.133"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(56..1561)
                     /locus_tag="ACHGMT_002582"
     CDS             complement(56..1561)
                     /locus_tag="ACHGMT_002582"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFN6836281.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLEFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(1653..2243)
                     /locus_tag="ACHGMT_002583"
     CDS             complement(1653..2243)
                     /locus_tag="ACHGMT_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN6836282.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            2422..4068
                     /locus_tag="ACHGMT_002584"
     CDS             2422..4068
                     /locus_tag="ACHGMT_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFN6836283.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            4117..4836
                     /locus_tag="ACHGMT_002585"
     CDS             4117..4836
                     /locus_tag="ACHGMT_002585"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFN6836284.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            5315..>5532
                     /locus_tag="ACHGMT_002586"
     CDS             5315..>5532
                     /locus_tag="ACHGMT_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFN6836285.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFK"
BASE COUNT         1667 a         1006 c         1122 g         1737 t
ORIGIN      
        1 aaatcacagt cccttttttt cttagaaatg ggaaaccact agtatataaa taccattatt
       61 cttcaatcaa ccgcttcacg attgcataat ccccttcata aggttcatct acaccattga
      121 ttttttgata gggatccacg ccttttcccg caataacgac tgaatcttgc ggagcgcttt
      181 ctgctaaagc gcgtcgaatc gcttcaggac gatccatttc ataatgaacg atcaaatctg
      241 gattagtaat tgcttcattg atttcttccg cgattttttt cggatcttca aatgctgggt
      301 catctgccgt caaaaaggcg atgtcagctg tttctgatag cacttttcca aaatcatgtc
      361 gacgggagat tgctttgttt cctgggctgc ctaatacaac aattacacgg ccatctggat
      421 gttcattttt ggcaaattcc agcaatgttt tcaagcttag ataattatgt gcatagtcca
      481 catagacatg cgctccattg gattggatca attgatccat tcggcctggt acacgagctt
      541 cttttaatcc gtcttgtgca tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg
      601 cactggctgc attttcttgg ttgaatcctc cagccagcaa aatatcataa gcagtatcgc
      661 agatagctaa gcgatcttca tgagaagata aagtaaaacc gtgagtacct ttatctgaac
      721 ggatcacttg ataattgctt tcttccgaac gaccataagt gatcgttgga atctggaata
      781 gatcggctgt ttccctaagt aaatcaaaat agtcactttc atgatttaaa acaactgttt
      841 ttgaattcaa gatcaattgt cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga
      901 tcggactgat atggtcaggc gagatattca agaagattcc gacatctaaa gtcaagccgt
      961 atacgcgttg tgttttgtaa gcttgtgaag aaacttccat cactagatga gtcataccat
     1021 tttcaactgc ttctgacatc atacgatata aatctaatga ctcaggcgtt gtcaaatggg
     1081 atttgaaata tgttttgcca tctaacgttg tattcatagt agaaagcaat gctgtttttt
     1141 tatttgttgt gtgatccaag attgcttttg taaagtacgc agcagttgtt ttaccttttg
     1201 ttccagtgaa tccgatcact ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa
     1261 gaagcgccat tgctttacgg atatccgtta cgatgattcc tactgctgaa ttttcgtatg
     1321 gttgttctga tacataatat tgaataccct gttcgattgc tgaatccaaa tattcttctt
     1381 tgaaattcat tcctttacag aaaaacaaag tagaagcatc agcttttctt gaatcataac
     1441 ttaactgaag aaattcagct ttttctggta catcaagatg ccagcctctt gttgaaacaa
     1501 attcttttaa tagattttct tttaatagta aatgacggat gtcttctaaa gagaagctca
     1561 ttgattgatc cctcgcttta ttcaatttct tggtttatta tagcacactc tttttaatat
     1621 atcgtttgtt tccgctatga caaaatccag ctttattcaa attttcttga cacaggggac
     1681 aagcggatga gtattttcat aaacaatgaa ctgactgtaa tagataggag aacgaatagt
     1741 gtctccaaca atccaaaaac ataataattc aaaaagacga taagactatt ggtaagtaaa
     1801 agtccttgcg gcattggaag ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata
     1861 ccgccatttg aggccccgac tgtgtacaat gtgtaaaccc caagactgaa caaaagactt
     1921 cctgaaatca gtgaaaagaa atgtgaggag atcatttcca cttttggaac aatctctaaa
     1981 acaacaggaa aaagcagact tcccgctaca gtcttaaaaa atactgattt tcctaatagg
     2041 acagcggcta gaagcagcat cataagattg atgctccaaa cagtaactga gcgttggata
     2101 gaaaaggccg tttcaactaa atgtccgatg gcgcccactc cggcggaagc tatatcgtga
     2161 gggagcaaga accaattcaa tccaactgtg ataaagaaaa gacctaaaaa tataacgggt
     2221 agttttttta tttcttgtgt cattgatatc tcctataaat gttagccata aaaggctgta
     2281 tatttcttta ctttaccaaa aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa
     2341 agttatataa aatcgattgc cagtttctgt tttaccaatt aaaatgatag taatgttttg
     2401 tagaaagttg gaatctgacc gatggataat caaagaaaac cagcattaga tcaattaaca
     2461 ccgcaagaaa aaatggcacg aggctctgct tggatgaccg ccagtaatat catttcaaga
     2521 ttgctaggcg ctgtctatat cattccctgg tatgcttgga tgggcgaaaa tgctaaggct
     2581 gccaatggat tattcaatat gggatacaat atatatgcat tgtttctact gatctctaca
     2641 gcaggaatac ctgctgcgat tgcaaaacaa actgcacgct ataattcatt gaatgaatat
     2701 ggcacttcac gacgattatt tatccgtgca ttacagatga tgggcgggct aggcctgttg
     2761 tttgctttgt tcatgtacat cgcctctcct tggctagctc atgcttctgg tggcggagaa
     2821 gaactgattc caacgatgcg ttctttgagt atcgctgtat tggtttttcc ttgtatgagt
     2881 gtgatacgag ggtattttca aggcaatcaa gaaatgatgc cttatgcgct gtcgcagatt
     2941 gtagaacagg tggcacgtat tttttatatg ctattggcta cattcatcat catgaaagtc
     3001 atggatggtg actatgtgac ggcggtcacg caatcaactt tcgcggcatt tatcggtatg
     3061 ctagcaagta ttcttgtcct tttgtatttt ttgaagaaac atcaagcgta tacatcggcg
     3121 tttattcact acagcgaaaa caaaataaat attgcaacga aggaattgtt gttagatacc
     3181 gttaaagaag cgattccgtt tattattgtg ggttcagggg taacgatctt caaattagtc
     3241 gatcagttca cttttatgaa gatcatgtct gcatccaccg agtattccaa tgcacagtta
     3301 ctggatcttt tttcgatttt tagtgccaat ccagataagt tgacgatggt agttattgca
     3361 ttggcaacat cgatcgcgtc tactgggtta ccgttgatta cagaagctgt tacgttgaaa
     3421 gatcgagtag gtctggcaaa attgacaagc ggaaatttac agttgttttc attttttatg
     3481 tttccggcga catttggtgt tatgttactt gcctatccgc taaatacatt attttataca
     3541 ccggattctc taggaagtaa tgtactgatc caagcaagtt ttgtaggact cttcctagga
     3601 ctatatatgc tagtttccaa catgctgcaa ggaatgtttg aaaacaaggc ggcaattaat
     3661 tatttggtta ttgggtttct agtgaaactc cttcttcaat acccagccat tcgtttattt
     3721 gaagtatacg gccctttatt ggcgacaatg atcggatttg ctgtgtcttg taccttgatc
     3781 ttaaagagaa tccatgaagt agcccgcttt aaaccaagtt ttgtctggcg caggacattg
     3841 ctgatcttta ttcttacctt gattatgctt ttggcttcgt ggatcacaaa gatgattttt
     3901 ggtgcttttt taaacgaaga tagcaaattc cagtctctat tgttgattct tgtagtagca
     3961 ggtatagggg ctttagtgta tggttattta gctttgaaaa tccgcttggc agaaagacta
     4021 ctaggaccag gaatagcgcg tttacgacat aaattaaaaa taaaataagt gaatcagaag
     4081 agctagattg atgctctttt gaaatggggg aaacgcatgc gtttagataa gtttttagca
     4141 gatatgggac ttggcagccg caaagaagtc aaaggattga tcaaaaaagg aacgatcgaa
     4201 gtcaatggac aagtggtgaa aagcgataaa gcgcaagtaa atgaattcga agatgcggtt
     4261 acttatttag gagaaatcat tacgtatcaa aaagattttt actacatttt gcataaacct
     4321 gcaggagtca tttctgctac tcaggataat catgatcaaa ccgtaatcga tttattggaa
     4381 gaccaggatt atcgggaaga tctttttcca gtcggccgat tagataaaga cacagagggc
     4441 ctcttgattt taacgaatga cggggtgttt gcccatcagt tactatcacc taaaaaacat
     4501 gtggagaaag aatatctagc tgaggttagc ggtataatga cagaagaaga tatccgttta
     4561 tttgcagaag gactgattat tgatggcgat gagcagacat tgccggcaaa actccaaatt
     4621 gatcaagtag atcaagagac agaaacttca attatccgtc tgattttgca tgaaggaaag
     4681 tttcaccaag ttaagcgaat ggtcaaagca gtaggaaaag aagtcttata tttaaaaaga
     4741 atccgtatgg gaaaattttg gctgcctaaa gatttgaata aagggcaata tcgacccatg
     4801 accaaagaag agctagaaca agtgagagaa aaataattga atggatagct aaaagaataa
     4861 gcagagtagt tgtagcgcca tctgaaaagc agacttgtcc gtctgacttt cggatggctt
     4921 ttttgtgaaa tttcggctta tttccgtaga agctacttct ggaacaccgt ttgttcggaa
     4981 taagggactg tgacaagact tttgttacag tccccattgt tccttgtgac tcagagttag
     5041 atagtaagga cacgatctcc ctaatactca ggccaaaacg ctgaattcac aacctctgat
     5101 aaacggtgtt caatcagctg accctcggta ttagctcaaa aaacggcaaa atatgaggaa
     5161 ctattttccc gtttttttct ctaatactca ggccaaaacg ctgatttcac aacctctcct
     5221 atactttgtt tcattttccc tatactttgt ttatttattc tcattattga aagcgctata
     5281 attacgttgc gaatgaatcg aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca
     5341 tttttttatg aatctatggc gttataaagc acttgtcctg atggctgtac cgggtatgct
     5401 ttggatgatt tttttctttt acatacctgt aatggcgaat gtcgtagcat tcaaaaattt
     5461 tcatatttca gcagatgggt tcttggctag cttgagggaa agtccttggg tgggttggga
     5521 aaatttcaaa tt
//
LOCUS       DAZBSA010000133         5253 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000133 DAZBSA010000000
VERSION     DAZBSA010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.134"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACHGMT_002587"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACHGMT_002587"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFN6836286.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="ACHGMT_002588"
     CDS             complement(1297..2133)
                     /locus_tag="ACHGMT_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6836287.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="ACHGMT_002589"
     CDS             complement(2317..3534)
                     /locus_tag="ACHGMT_002589"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFN6836288.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3844)
                     /locus_tag="ACHGMT_002590"
     CDS             complement(3683..3844)
                     /locus_tag="ACHGMT_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836289.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3917..4000
                     /locus_tag="ACHGMT_002591"
     tRNA            3917..4000
                     /locus_tag="ACHGMT_002591"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..5253)
                     /locus_tag="ACHGMT_002592"
                     /pseudo
     CDS             complement(4240..5253)
                     /locus_tag="ACHGMT_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1625 a         1074 c          835 g         1719 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca
     4921 gcacgtaatg cattccctgg agttgtcaaa gagaaaatta attctgctaa gcttattatt
     4981 gaaaatactg ataacttata attatatcgt ccattgacaa tcaaatataa actaactata
     5041 cttgttagaa caaaaaaaca ggcgttgact tgttcttgat tagttaaaaa tatcaataaa
     5101 ggaatactta cccaatatat ttttctattt atttcttctc ctcttaagag ttgaaaaaac
     5161 ggataaaaag ttagtaaact aaatgctact ggccactgat agttcaaggt ggttgctctc
     5221 caaccggttt cccccattac tgtcaatggt att
//
LOCUS       DAZBSA010000134         5169 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000134 DAZBSA010000000
VERSION     DAZBSA010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.135"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..963)
                     /locus_tag="ACHGMT_002593"
     CDS             complement(<1..963)
                     /locus_tag="ACHGMT_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFN6836290.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDGRIS"
     gene            complement(1034..1330)
                     /locus_tag="ACHGMT_002594"
     CDS             complement(1034..1330)
                     /locus_tag="ACHGMT_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836291.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            complement(1323..1616)
                     /locus_tag="ACHGMT_002595"
     CDS             complement(1323..1616)
                     /locus_tag="ACHGMT_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFN6836292.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(1762..2313)
                     /locus_tag="ACHGMT_002596"
     CDS             complement(1762..2313)
                     /locus_tag="ACHGMT_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFN6836293.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(2487..2786)
                     /locus_tag="ACHGMT_002597"
     CDS             complement(2487..2786)
                     /locus_tag="ACHGMT_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HFN6836294.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            complement(2813..3121)
                     /locus_tag="ACHGMT_002598"
     CDS             complement(2813..3121)
                     /locus_tag="ACHGMT_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFN6836295.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            complement(3238..4197)
                     /locus_tag="ACHGMT_002599"
     CDS             complement(3238..4197)
                     /locus_tag="ACHGMT_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN6836296.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            4341..5117
                     /locus_tag="ACHGMT_002600"
     CDS             4341..5117
                     /locus_tag="ACHGMT_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN6836297.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
BASE COUNT         1540 a          958 c          811 g         1860 t
ORIGIN      
        1 agaaattctt ccatctgtat aaacacggaa ttgttttcca ccagccattg ttgtatttcc
       61 attatcatcc gttacaatat taattgaata gtagcttcca ttttcatctt gcattagatc
      121 tttcatatat tcatttgtgc taattaaatt agtatttgat gcatttttat caggagaatc
      181 atacaaccat aaagctttat tatttagtac agtttcttta gcagcttccc aacttgaaat
      241 tgctgagcta cttgtagatt gtgtcgttgt agttgttgct tgttgagtag attcttctac
      301 tactggagtt tgaggagttt gtccattcac aatatcaatg aaaccttgtt ttaactgttg
      361 gttctgagtt tcattgaagt acatgtagtt tttatcattg ccttgatttt gttgcgttaa
      421 ataaacttta ggttgtccat tttgaaagcc aaagaaatat acatgctgcc ctaaatattc
      481 tccagtttct acatcagaat aaactgctac taaattgaaa tctgcatgac cagttccatc
      541 ttctgaccat tgtacggtaa ctggtgcttc attgattgcc attttccaat ttccatcaat
      601 aactgcactt ggtaaaggcg taccatacag acttacattg ttctgaggat tgtagctttt
      661 atattcttga cctaatgtct ttccccattg tgggacaaaa attgctagtt gagtagattt
      721 ctcactatcc cacaaagtat ttttaacctc ttcagaagaa gattcaatga ttgaactttc
      781 acttgtactt gcttccacag attgtttcat ttctgttgta gtttgttcag ttgattgcgt
      841 tttacttttt ttagcctcaa ttgttgtatc agtttctaca gtctctttag tcctcgtatt
      901 ctcagatgta caaccagtga ataaaatccc cattccaatt agtaataaca accatttttt
      961 cattttattt ccccctttca atttctccct cgcgtactta ttttatcagt caattaatct
     1021 caatggtgtt catttagtta atcctttccc aagaatgtcg atagccagca tgacaacgat
     1081 ctcttccttg agggctttca gatggattaa aactagtaga aatttccctt ccacatcctc
     1141 tacagcgata agttctcatt atttttgggc tattagtatt atttttctta gttagagcta
     1201 tgagtccaac tatgattacc ccaactgtct taattacttt tcctttatct atattcggat
     1261 gcgcaagcat atatttatat aaccaatatc caccgtaaat taaaccacct aatacaatca
     1321 gcttagccat gtactcgtct cctttctttt tgtttaatta ctttttgaat ccatcttgca
     1381 gagaaaccat aaacttctgt caattgacgg atatctacag ttttttcctg ttctatcatg
     1441 ttttcgattc tttttctaac taactcacga tcgtacattt tcattggaaa agaaatctgt
     1501 tgccctcgaa aagcaagata aaattgatag agcaattctg aatccagttc catttcctca
     1561 aaaaagtcat tcatactttt ataagagtta ctcaaacaag tttcatcaat ctccatctac
     1621 acaccccctg ttatttttta cataatgaaa atagcatatt actgggtaca ttgataattc
     1681 atataacatg tatcatcaca tgtattttta aagtcaaaaa gcatgtaaaa ctacttataa
     1741 gtaacataag aaaataaaat gttatttttc ttgaaataat aattgactat ctgccaattg
     1801 ccattcatta cgaacatgaa tacttagtgg gtgggataac ttaccttctg catttatcaa
     1861 tactttggtc atcttttttt gtttcgcact caattgatcg taaatggtct tcgcttgttc
     1921 ataagccact tgactcgtcg ctgtaatcga accacaagca aaaataatgg tgttgatttt
     1981 cttttctgtg aaaacagttg ttaatatttc caaagattgt tcatcatttc cacgaggaaa
     2041 tacccgagga cttttcgctt ttgtatacga aaaaagattc acagcaataa aaccagtaaa
     2101 tcctaattgt cggatattct tttcgattaa taacatactc aaatcattcg taaatggttc
     2161 tgtatctgtt ggacgaatag taatgactac cgctaattta tcttccgctt cttttccttt
     2221 gatcgcccat tgtcgtttga aaacatagcg atgggtatga tgaggatctc gccaaacttc
     2281 tgtttgtgtg gtcactaatt ctttttcaat catctctttt tctcctatgc taatttttgt
     2341 tcaagcaatt caatcaccat tgccgttata agtgtcaata ctagttgaaa atatttcata
     2401 ttctttctcc ttttttcttc agaatttgat gaatcgctgt aaggatatct tctaaacatt
     2461 tcgcaatcat tctcaaataa tcgatcttat tcattcgttc ctccaatctg actccaaacc
     2521 aatcgtcctt ctaccagtaa cccacgaaca aattctcgct ggctggtaat atctgtaatg
     2581 aaataaacta catagtcttt tccatcttct ggtgcgcaat aaatataaag atgtgtcatc
     2641 actaaacgtg gtagagtaac atcttcagga agcataccca tttcaatttc ctctttttcc
     2701 atcgtaacgg attcatagcc ccactcttca ggagtagtga ctagctgtgc acacttttcc
     2761 agtacttcga tagccgtttc taacaagtcc tttcgttcct ttcttttaga tatcagtatt
     2821 cttctggtaa taacatcgtt tgatactcac ccgaatcaat gacccatatt ttttgagaca
     2881 ctgatttcaa acgaagctta ggtgaaatca gatactcttt tcttgcttct ggttcttctt
     2941 gacgatgaac caacttttga tttccttttt ccgtagaaaa ttcaaagatt tgaagatagt
     3001 ctaaatgaaa ttgcttttga acgttttgat cgattagatt ccaacataaa gcttgtaatt
     3061 ccaccggcaa actgtccatt actccctttg tgacataacg ttgtccttta ttttgaaaca
     3121 tttcttttcc ctcctttgcc ataaaaatag cccaatcttc tattaaagaa gattgggctt
     3181 catggatata atatatattt gatattcaat ttgattcatt ttatactatc tgatttgtca
     3241 aattaagcta gacaatatgt ctttatctat gcagctcaaa aatacttcca atggtgttcg
     3301 ataatttaat gattttcttg gaatatggtt tcttttcgag gcaacggatt gaataaaggt
     3361 ttcatccgtg tccctaaagt ccattgtttt aggtaagcca tctcgtctaa gcaagccgtt
     3421 tgagtgctca tttagccctc tttgcgaagg tgttcctgga tctgcgaaaa agatggcaat
     3481 atcgttcgag ttacttatag atcgccagtt ggaaaattct tttccacaat caaatgtcat
     3541 ggatttaaac aagtgtttag gcatggattt gaaccaatca tttaacctgt tttcgatatc
     3601 ttcggctcgt ctgcctgtcg gtttcaaagt aataataacc ttggataaac actcaaccaa
     3661 tgtaataaca gcgctcttgt gtttaactcc cacaatggtg tcgacttcga gatgaccaaa
     3721 ttctttagtc aggtgtggat agagactgtt ccgttcatga atcgttctgc gaaaggcctg
     3781 ttttccacgc ttttcttgat gattattcgg tttacgtttc cccttcatag gtaacgactg
     3841 tgtgtcaaaa aggccttttt taaacatccg atagagtgta cgaacagaac aatcaattgg
     3901 aaactcgcca cggccgataa tcacatcggg tgaccatcct tgagctacct tttcttgaac
     3961 atatgttgat tgttcatcag ataaaacggt tgaacgtcgt ccacaacgag atttattttg
     4021 tttgtattga cggtaataat ctagcacttt ttttccttgt ttgagaaacg aataaacatt
     4081 ataaatggtt tgtctagagc gatttaaact ttgtgcgaca agagcgacag gtttctcgat
     4141 attgaaataa gactctatga ttactagttc gtctgttgta agatgggtgt aagtcatttg
     4201 tactcactcc ttattaattc tttggtcgga actattttga gtgtagcaca aatgatcttt
     4261 ttagttgtct agcttaattt tacaatcgac gttttaataa attgtgatat aatgaagtat
     4321 aactttttag ggagtgtact atgaattttc aagataatag accattatat ttacaagtag
     4381 aagcttctat acgtaacgat attcgtgaaa aaaaatatct tccaggagag caattaccta
     4441 cagaagaaga attatgtaaa atttatggag taagtaagat taccattcga aaagctttta
     4501 aacttttagc agaaagtgga acagttgaac gattaagggg gaaagggacc tttgtaactc
     4561 aaaaaaaaga aagtttaaaa ttagctggag ttcatggatt tagcgattct ctttctaccc
     4621 gaggacataa aattagatat actgttttac attctaaaat tataaaggct gatgagtttt
     4681 tagctaataa attacaaatc aatgtaactg atagagttta caatattaaa cgtttaatgt
     4741 gggaagacgg tgccccgatg ggaattgata attttttcgc atcagaagaa aaatttccaa
     4801 ctttatttga aaaaggtagc caatatactt cattatataa acttctagaa gatgactatg
     4861 atgtcaaagc tacgagctct attatggaaa taaacggttt atctgcaaca cctgagcttt
     4921 ccgacttatt gcaatgtata gtaggagatc cgctatttat tttagaaaag gttggcaaag
     4981 ataaaaataa tcaaccattt cattattctc aatccacagt tcgttgtgat agaattagtt
     5041 atgttattga agtcacagaa aatatcactt tagatacggt gcataaaaat gattcagatc
     5101 ctaaagtaat agattgatat taaaagaaat catacccaaa gcaatcaatt ttgattagat
     5161 ttgggcatg
//
LOCUS       DAZBSA010000135         4972 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000135 DAZBSA010000000
VERSION     DAZBSA010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.136"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            87..572
                     /locus_tag="ACHGMT_002601"
     CDS             87..572
                     /locus_tag="ACHGMT_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFN6836298.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(676..1269)
                     /gene="clpP"
                     /locus_tag="ACHGMT_002602"
     CDS             complement(676..1269)
                     /gene="clpP"
                     /locus_tag="ACHGMT_002602"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFN6836299.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            1449..1976
                     /locus_tag="ACHGMT_002603"
     CDS             1449..1976
                     /locus_tag="ACHGMT_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836300.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            2067..2804
                     /locus_tag="ACHGMT_002604"
     CDS             2067..2804
                     /locus_tag="ACHGMT_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFN6836301.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            2808..3725
                     /gene="miaA"
                     /locus_tag="ACHGMT_002605"
     CDS             2808..3725
                     /gene="miaA"
                     /locus_tag="ACHGMT_002605"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFN6836302.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            3727..4956
                     /gene="hflX"
                     /locus_tag="ACHGMT_002606"
     CDS             3727..4956
                     /gene="hflX"
                     /locus_tag="ACHGMT_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFN6836303.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         1691 a          850 c         1111 g         1320 t
ORIGIN      
        1 tttttttctt atgaaataaa tatatgctat gataccttta ttcaatagga ttagtttcta
       61 caaaaaatga aaggagaaaa ataaatatgg aacggtttat agcatcaaat gccacagtga
      121 ttggtgatgt gacattgtca gaagatgtga cgatctggta tcaagctgtt ttacgcgggg
      181 acagtaattg gataaaaata ggacaaagaa cgaatatcca agatggtaca atcatccatg
      241 tcgatcatga tgcacctgta gatattgcgg agaatgtgac ggtaggacat cagtgcatgc
      301 tacatggctg tacaatcgaa aaaggggcgc tgatcgggat ggggaccacg atattgaatc
      361 atgcggttat tggtgagaat agtttgattg gtgctggatc attagtaaca gaaggaaaag
      421 ttattcctcc taatgtcttg gcttttggtc gcccagctaa agtgatccgt cctttgacaa
      481 aagaagaaat acaaaaaaac aaagaaaaca tccagcatta tgtcgagata ggtcggatgc
      541 atgcgcaagg aaaattcaag gagcaaagat aggcagtagc atccataaaa taaaaaagga
      601 tggtcagttt gagttgacca tcctttttta tttggtttag ataagagagc ttaaaaaagc
      661 ttcctttgaa acagattaat ttaatgaaga actgttttcc atgacttcat cgatcaatcc
      721 atatgctttt gcttgttcag ctgtcatata gttgtcacga tctgtatctt tttcaatcac
      781 ttcaagcggt tgtccagtgc gttcagctaa aattttgttt aagcgttcgc gtgtttgcaa
      841 aatatggcgt gccgcaattt cgatttcagt tgcttgccct tgagcgccgc caagaggttg
      901 atggatcatg atctcagcat ttggtaaagc aaatcgttta ccttttgtac cagctgtcag
      961 caagaagctt cccattgaag cagccatacc cataacaatt gtttggacat ctgctttgac
     1021 aaagttcatt gtgtcgtaaa tagccatacc tgctgtaact gaaccaccag gagaattgat
     1081 ataaagatag atatcttttt cagaatcttg agcgtctaag aaaagcaatt gggcgatgat
     1141 cgaattagca agatcatctg ttacttggcc gcttaacatg atgatacggt ctttcaataa
     1201 acgtgagtag atatcgtaag ctctttcacc tcgtgaagat tgttcaatga ctgtaggaat
     1261 taaattcata atagagttcc tccttgattt cgtttagaaa tagtgtacca taatttagtt
     1321 tctaatacct aaaatgtagt atacattttt ggtcaaaaaa ggtcaaataa aaaacttagg
     1381 caaattagtc gcataaaccc gctttatggt atgataagca gagaaacaaa acaaggagga
     1441 acaacagatt gatagttggt attactggcc ttatcggagt gctgacaggt gctgtagttg
     1501 tctatatcgg catgatactg caaatgcgtc acttggaaaa taaagaacag cgtagacgtg
     1561 aattggaatt gaaagtcaaa gaaatcgaga cgctgaatca attaaataaa aagatcaacg
     1621 agatcttgca aaaacggaca acgctgatgg aagaatatgt cagctttgat gcatttgatg
     1681 attgttatat cacgattgac gattttgcgt atctccaatc attttcagcg caaaatagtt
     1741 tttatctgcc aaattatatt ttagacgagc tatttaagaa tatctcccat cgaaaagtga
     1801 ttttatctcc agatgaaaca atgaagattg gtggatatgc ttataaaggc ggacgcgtga
     1861 ttttagagaa ttttacggat agtctgactg aaatgatcaa tgaaaaaaaa gctcagttga
     1921 agactatgac gcgccagccg attggtttct tctcgaaaga tgatgtttgg agctgatatt
     1981 ttgaagcatt gccgttgcac atgagagcgt ggtaatgctt tctttttttc ttaatgatac
     2041 aatagaactg aacggagtga agaaagatgt tacaaatcta tgcccatcgc ggaagcagcg
     2101 gcacccaccc agaaaatacg cttcctgcat ttgcagaagc tgtacgagta ggtgcagatg
     2161 gtatcgaact agatgtccat ctaagcaaag atggttattt gatcgttatg cacgacgaag
     2221 aagtggaccg gacgactaac ggtaaagggc tgattcgaga aaaaacattg gaagaactca
     2281 aaaaattgaa tgcagggggc aaattcagca aagaatttcc agcagctaaa gttcctgaat
     2341 tgagagaagt gttaggactg ctcctccaaa aaaattaccg agggatgcta atgattgaaa
     2401 ttaaaactga tcagtatgaa tatcccggta tcgaagaaaa attagccgaa acattaaaaa
     2461 gtagagattg gccttttcaa catgcatatt gcagctttca tttggattct ttggaaaaaa
     2521 tcgcattact ggaaccggag atacagctgg acttattgat gagtacttct gataaaaaga
     2581 tccaactagc agcagaaacc ccttatatag aaggagtaca tccaaaaaat acttggttgt
     2641 tcgaacatcc agtccaggca gctgattttc caaaagcaat tcgtgcatgg acgattaacg
     2701 atgaagaaga aatggccaaa tgtcaagaac tcaaaataga tggaataata actgattttc
     2761 cggagaaagc tcttcggtgg aaacaatcaa cgaaaagtag gtaaaatatg gaaaaagtat
     2821 tagtgatcgt cggtccgaca gcagtcggta aaacagcgct aagtgtggaa ctcgcacaac
     2881 tatttggagg agagatcatc agtggtgatt cattgcaaat ctataaaaat ctggatatcg
     2941 gtacagcaaa aatcaaagaa gaagagatga ataatgttcc gcatcatttg attgatgtga
     3001 tcgaacctac tcaacgttac tcagccgctg attttcagca ttcaggtcga caattgatta
     3061 ccgagattgc gaaccgaggg cacctaccta tcatagctgg tggtacgggt ctctatatcc
     3121 aatcccttct atatgattat cagttaggag cgacagaaga agcgaatcct gagattagaa
     3181 aaaagtatga aacgttagct gaaacaattg gttcccaagc attatggaat cagttgaaag
     3241 aaaaagaccc ggcagctgct gagaaaatcc actggaacaa tcaacgcaaa gtgataagag
     3301 ctctggaagt tttagagacg acaggacata gtatcacggc acctaaagaa gagccaaagc
     3361 aattgtatga ttacctcatg attggattag atacgaagcg tagtattctt tatgaacgaa
     3421 tcaatcaaag agtcgatttg atgctagcag aaggtttagt agcagaagcg cgtaaggtgt
     3481 atgagcttgg agaagtccag gccagtcaag gtattggata taaagaattt ttcccttatt
     3541 ttgaagggaa agaaacattg gaagaagcaa cagaacaagt caaactacat tcgagaagat
     3601 acgcgaaacg tcagttgacg tggtttcgta accgtttgga tgcacagtgg tttgatctta
     3661 taagtgagcc aaatcaaaaa agtcagattg agcaaacaat caaaaaatgg ctggaggcgg
     3721 aataaaatgg aagaaaaagt gatcatcgta ggggtagaaa ccgaagcaaa ccagcgttac
     3781 ttttcagagt caatggaaga gctagttcag ctgacgaata ccgcatcagg agaagtcgta
     3841 ttcacaatta cacaaaagag accacaagtg gatcggcaaa cgattatcgg taaagggaaa
     3901 ttacaagaat tagttcaaca agcggatgcg catgaagcgg atttgatcat ctttaatcat
     3961 gagctaactc cgagacagag ccaactgatt accgatgctg ttggtcttcc ggtaattgat
     4021 cgcgtgcagc ttatcttaga tatttttgcg atgcgtgctc gttcgaaaga agggaagctg
     4081 caggttgaac ttgctcagtt agaatacctg ctgcctcgtt tagtagggca agggaaaaca
     4141 ttatctcgtc ttggcggtgg gatcggaaca agagggccag gtgaaacaaa gttagaaaca
     4201 gaccgccgac atatacggaa taaaattttt gctgttaaga aagaattgaa agaagtagaa
     4261 gctcatcgag agcgcaatcg ccaaaaaaga aaaaatagcg agatttttca gattggactt
     4321 attggctaca cgaatgccgg gaagtcaact atcttgaatc tattgacaca agcggatact
     4381 tattcgaaag atcaattgtt tgcaacgtta gatccattga ctaagagatg gcgttttgct
     4441 gaaggatttg agattactgt aacggatacc gtcgggttca tccaagacct gcctacgcaa
     4501 ctgatcgatg ctttccattc tacattagaa gaaagtcaaa atatggatct tttgctacat
     4561 gtagtcgatg ctagttctcc agatcgtatt ttacaggagc agactgtctt aaaattgatg
     4621 gatgaactga atatgaaaga aatgccgata ctgactgttt acaataaggc agaccagatc
     4681 gacccagcga tgttcacgcc tacgctattc ccaaatgtat tgatttctac tcagacgaaa
     4741 gaaggtaaga tggcgttgat cgaagcaatc aaacgtcagc tgatggaact aatgacccct
     4801 tatacacttt ctgtgccaag caacgaagga cagaaattaa gcgagttgag aagacaaaca
     4861 atggttttgg acgaagcgta catggaagaa acaaacgaat atgaagttaa agggtttgca
     4921 aaaaccaatt caaaatggtt aagaaaaaac caataaaatt ttattttttt aa
//
LOCUS       DAZBSA010000136         4964 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000136 DAZBSA010000000
VERSION     DAZBSA010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.137"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACHGMT_002607"
     CDS             complement(<1..182)
                     /locus_tag="ACHGMT_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFN6836304.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACHGMT_002608"
     CDS             517..951
                     /locus_tag="ACHGMT_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFN6836305.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACHGMT_002609"
     CDS             974..1294
                     /locus_tag="ACHGMT_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836306.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACHGMT_002610"
     CDS             1435..1626
                     /locus_tag="ACHGMT_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836307.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACHGMT_002611"
     CDS             complement(1697..1954)
                     /locus_tag="ACHGMT_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFN6836308.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACHGMT_002612"
     CDS             2112..2375
                     /locus_tag="ACHGMT_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836309.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACHGMT_002613"
     CDS             2399..2587
                     /locus_tag="ACHGMT_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836310.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACHGMT_002614"
     CDS             complement(2670..2855)
                     /locus_tag="ACHGMT_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836311.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ACHGMT_002615"
     CDS             3070..3441
                     /locus_tag="ACHGMT_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFN6836312.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="ACHGMT_002616"
     tRNA            complement(3591..3663)
                     /locus_tag="ACHGMT_002616"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="ACHGMT_002617"
     CDS             complement(3709..4137)
                     /locus_tag="ACHGMT_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836313.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="ACHGMT_002618"
     CDS             complement(4197..4955)
                     /locus_tag="ACHGMT_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFN6836314.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1735 a          776 c          975 g         1478 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atct
//
LOCUS       DAZBSA010000137         4931 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000137 DAZBSA010000000
VERSION     DAZBSA010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.139"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..972
                     /locus_tag="ACHGMT_002619"
     CDS             85..972
                     /locus_tag="ACHGMT_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFN6836315.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="ACHGMT_002620"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="ACHGMT_002620"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFN6836316.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="ACHGMT_002621"
     CDS             2274..2720
                     /locus_tag="ACHGMT_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFN6836317.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="ACHGMT_002622"
     CDS             2993..4345
                     /locus_tag="ACHGMT_002622"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFN6836318.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="ACHGMT_002623"
     CDS             4516..4803
                     /locus_tag="ACHGMT_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFN6836319.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1695 a          813 c         1030 g         1393 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt t
//
LOCUS       DAZBSA010000138         4906 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000138 DAZBSA010000000
VERSION     DAZBSA010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.140"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            105..923
                     /gene="purR"
                     /locus_tag="ACHGMT_002624"
     CDS             105..923
                     /gene="purR"
                     /locus_tag="ACHGMT_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFN6836320.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1426..2799
                     /gene="glmU"
                     /locus_tag="ACHGMT_002625"
     CDS             1426..2799
                     /gene="glmU"
                     /locus_tag="ACHGMT_002625"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFN6836321.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2893..3864
                     /locus_tag="ACHGMT_002626"
     CDS             2893..3864
                     /locus_tag="ACHGMT_002626"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFN6836322.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3870..3972
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4038..4607
                     /gene="thiT"
                     /locus_tag="ACHGMT_002627"
     CDS             4038..4607
                     /gene="thiT"
                     /locus_tag="ACHGMT_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFN6836323.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4688..>4906)
                     /locus_tag="ACHGMT_002628"
     CDS             complement(4688..>4906)
                     /locus_tag="ACHGMT_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836324.1"
                     /translation="NLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIWKLKGTRYREEE
                     IIATLIYSVTDRHWQLVEDADLSYLPLK"
BASE COUNT         1604 a          864 c         1073 g         1365 t
ORIGIN      
        1 taaataccga actattttct tattttttca taaaatgctc gaatttttga agaaaaactt
       61 atataatgta aaacgataaa atttcttaaa aggagttgga ataggtgaaa gtacgtcgca
      121 gtgaacgtct agttgacatg acacagtatt tattgaatca cccgcatgaa ttgatttcct
      181 taacaagctt tgccgaacgt tatgaatctg ctaaatcttc tatcagtgaa gatttgggaa
      241 tcatcaagaa aacatttaaa gaacgagggt atggaacctt ggaaacaatc ccgggtgctg
      301 caggtggtgt attgttcatc cctgaaattt catatgaaga ggcgaaaaaa tatattgaat
      361 ctctagcaga acgtctatca gaacaagacc gtctgcttcc aggaggatat gtttatctct
      421 ctgatttgtt aggagaacct gaactgctgc gtcaagttgg aagaatcatt gcttctaaat
      481 accttggtga aaaaatcgat gctgtaatga ctgtagccac aaaaggcgta cctattgccc
      541 aagctgtgtc ctattatctg aatgcgccat tcgttattgt acgccgggac tcaaaaataa
      601 cagaaggatc aacagtaagt gtcaactatg tgtctggatc ttctgaaaga attgaaaaga
      661 tggaactgtc taagagaagt ttgaaacgtg gttcacgtgt ccttgtagtg gatgatttca
      721 tgaaaggcgg cggaacagtc aacggaatga agagcttgat tgaagaattc gaatcagaac
      781 ttgttggcat cactgtattt gccgaatcaa aattcaacgg tcatcgcgcc atcgatgatt
      841 atacgtcact gctttatgtc aaagatgtag atactcaaac gaaaaatatt acggttgtgc
      901 caggaaatta tttttcagaa taagaggttg tgaaaaagcc gcttagtttc gagtagtaag
      961 aaaaattttg tgaaaatggc tttccacatt ttttcataaa tttggcttaa taccgagaaa
     1021 ctggcttttg aacaccgttt atctagaggt tgtgaaatca gcgtcctgtc ctgagtatta
     1081 gctcaataaa cggaaaaatt gctcctcata ttttgccgtt tattgagcta ataccgaagg
     1141 tcagctgatt gaaaaacgtt taatgaagag gttgtaagcc taccgtttaa ctctaagtca
     1201 caagaaacga tttcaaaaaa cagcttttca tgttttatga gtagtgtgac ttgtgaccga
     1261 ggagttggca ggagagcacc ggttaccaaa tagataggcc cgttatggtc tatctatttt
     1321 tttatccaga aaacgctggg ataaaagaaa atcacttgtt ttgttgaaaa aatgggtata
     1381 ctgggataaa ctgaaatcag caaactatat ttaggagtga cgcagttgga cgcacgttat
     1441 gcaattattt tggcggcagg gaaaggtacc cgcatgaagt caaaattata taaagtttta
     1501 catcccgttt ctggtaaacc tatggtagaa catatcatta atcgggtcag tgaaacgaaa
     1561 ccagatgaag tgattacgat cgttggacac ggagcagaac aagttaaagc acaactagga
     1621 gaacgcagta aatatgcgtt acaagcagaa caattgggaa caggacatgc tgttttgcaa
     1681 gctgcttctt tcttggaagg aaaaaaagga acaacgttag ttatcagtgg cgatacacca
     1741 ttattgacta ctgaaacgct gaacaatctt tttgaatatc accaaggaaa aaatgcaagt
     1801 gcaacgatct tgacggcaca agcagaaaac ccaacaggat atggacgaat catccgtgac
     1861 catattggga tcgtagaaaa aatcgtggaa caaaaagatg caacaccaga agaagctctt
     1921 gtacaagaaa tcaatacagg aacttactgt tttgacaacg aagctttgtt cgatgcgtta
     1981 agcaaagttg gaacaaacaa tgctcaaggt gaatactatc taacagatat catcgaaatc
     2041 ttaaaagaag aaggccatac agttgctgct tatcaaacgg atgattttga agaatcaatg
     2101 ggagtcaatg atcggatcgc gttagctaaa gcaaatgaga tcatgagaaa gcgaattaac
     2161 cagatgcata tggtcaatgg cgtaagcttt gtcgattcag caacgacata catcgatgca
     2221 ggcgtagaaa tcggtccaga cacattgatc gaagcaggtg tacaaatcca aggaaataca
     2281 gtcattggtt ctgattgtgt catcgggtca cattcaaaaa tcgtagacag taggattgag
     2341 gatcacgtcg tcattgaaaa ctcagtcatt gaaagcagcc atgtcaagaa acatgcagat
     2401 gtgggacctt atgcacattt acgaccaaaa gctgaaattg gcgaaaacgt ccatatcgga
     2461 aactttgtgg aagtgaaaaa tgctcaaatc ggcaaaggaa ctaaagtcgg tcacttgact
     2521 tatgttggtg atgctacgct gggagaagaa atcaatgtcg gatgtggtgt tgtcttcgtc
     2581 aactatgatg gaaagaacaa acatcatacg actgtcggcg accacagctt tattggttct
     2641 agcacgaata tcatcggacc tgtcgaggta gctaaaaaca gttcgattgc tgccgggtca
     2701 acaattacag acaatattcc agaatatgcg ctggcaattg cgagagcaag acaagtcaat
     2761 aaagaaggat atgcaaaaaa actcccttat ctaaactaag acttttgcaa aatcacttga
     2821 tttatttaaa aaatatgact actatttatt aggtagaatt ctagagttgt gaaaaagaaa
     2881 gtggaggtcc ccatgtcaaa acattacttt gacccaagat taaaaatttt tgcgttaaat
     2941 tccaatcgcc cattagcaga gaaaattgca gcagcagtcg gcgtcgagtt aggaaaatca
     3001 tctgttactc aatttagtga tggagaaatt caagtaaaca ttgaagaaag tattcgtgga
     3061 tcgcatgtct atgttattca atcaacgagc agcccagtca atgataactt gatggagttg
     3121 ctgatcatga ttgacgcact gaaacgagca agtgcaaaaa caatcaacgt agtcatgcct
     3181 tattatggct atgcgcgtca agaccgtaaa gcacgtgcac gggaaccaat cacagcgaaa
     3241 ttagtagcaa acatgattca aaaagctggt gcaacaagaa tgctgacttt ggaccttcat
     3301 gcagtccaaa tccaaggttt ctttgatatt ccagtcgatc atttgatggg tgcgccactt
     3361 attgccaatt atttcttaga aaaaggaatc aaaggcgatg atgttgtcgt tgtttcacca
     3421 gaccatggtg gtgtcacacg ggcacgtaaa ttagcggaat ttttgaaatc tccgattgcg
     3481 atcatagaca aacgccgtcc aaaagcaaat gttgcagaag taatgaatat cattggacaa
     3541 gtcaaaggca aaacttgtgt actgatcgat gatatgatcg atacagcagg tacgatcaca
     3601 ttggctgcca atgctttgaa agaagctggt gcaactagtg tgtatgcttc atgtacacat
     3661 ccagtattat ccggccctgc tttacaaaga atagaagatt cagctatcga gcgtctagtt
     3721 gttacagact cgatctatct tccagaagat cgtaaaatcg acaaaatcga tgaagtaagt
     3781 gttggcggac taatgggaga tgcaatcaaa cgtatccatg aaaacaaacc agttagtcca
     3841 ttgtttgaaa caaaaaataa ataataaata aactcacaaa ggggagtcca attgggctga
     3901 gattgaatct atttctaaac ccttcgtacc tgtatcggtt atgcgagcgt aggaattgtg
     3961 aataaacagc tgtttgacta ttttcgaaag ctgcttcctc ctttgtgact tttttattca
     4021 aaggaggaag tttttttatg ggaaaaaata gagtttgggt agaaggaacg attgttgcag
     4081 cgttagcgat ggtgttgtcg ttgattccga tccagatcgg cagcagtttt tctatttcat
     4141 tagggcagat tccattgact ctttttgcgt taagaagagg gacaaaggca gggatgctgg
     4201 caggtttcat ttggggcatc ctacattttc cgttgggaca agtttattat ttaagtgtcg
     4261 ttcaagtgtt gattgagtac ccgattgcct atacttttgc aggcgcagca gggctcatgg
     4321 cttcaaatgt ccaaaaaggc tatgtagacg ataatacgaa acaaattaga aaaagcatcg
     4381 tgtttggtgc attgattggc gcactttcca gatatttttg gcattttatt gcaggagtgg
     4441 ttttctgggg agcgtatgca ctatggggga tgaatccttg gctcttttct ttcgtaatga
     4501 atggtgccag cgggttgagt acagctattg taacgatcat cgttttatta gggatccaac
     4561 aaacgactcc ttcgattttt gtgccagata aacgacagga gctgtaaata aaaaagaagc
     4621 aagaaacttg agacagaatc atgtcctagt ttcttgtttc ctttttattt tttgataaga
     4681 aatagatcta cttcaatggc agataagaaa gatcggcatc ttctaccaat tgccagtgtc
     4741 gatcggtgac agagtagatt aaagtagcga tgatttcctc ttctcgatac cgggtgcctt
     4801 ttaatttcca aatataatct tcaatcacat agattgggat acgatattta ccatcgatca
     4861 aaccaacttg atcaaaagaa aaaatgatcc cttcctgcat caaatt
//
LOCUS       DAZBSA010000139         4897 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000139 DAZBSA010000000
VERSION     DAZBSA010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.141"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            88..387
                     /locus_tag="ACHGMT_002629"
     CDS             88..387
                     /locus_tag="ACHGMT_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6836325.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            652..1527
                     /locus_tag="ACHGMT_002630"
     CDS             652..1527
                     /locus_tag="ACHGMT_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836326.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            1524..1742
                     /locus_tag="ACHGMT_002631"
     CDS             1524..1742
                     /locus_tag="ACHGMT_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836327.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            1729..4356
                     /locus_tag="ACHGMT_002632"
     CDS             1729..4356
                     /locus_tag="ACHGMT_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFN6836328.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFRDTLNDPNPLFDNLLKK"
     gene            4371..4589
                     /locus_tag="ACHGMT_002633"
     CDS             4371..4589
                     /locus_tag="ACHGMT_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836329.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
BASE COUNT         1905 a          609 c          982 g         1401 t
ORIGIN      
        1 aatacatttt tttacgcaat ttgtatttat ttgtacttat aaaggtagag gagttataga
       61 ataattaatt aaaaaggaga ggtttttgtg gaagcaataa aattaatgat ttcagaagaa
      121 caggaggaaa tgtttctaaa gcagttttat caaattgcta caaaagctat tgaaaaggct
      181 caagaaaatg cggggatgac aagggaatat ctcaatcaaa aagaaatggc ggaatactta
      241 aatgtgagtg aaaacttttt aaaaaatgct gtgagaaatg agggattgcc cgtagttgaa
      301 ttaggggcaa gaaaatttta ttcgaaaaag gcagttaatg agtttatgtt tgctcgaatg
      361 aaaagagggg aaaatattgg aagatgatga agtagaaatt ataaaaaaat gctttaaaaa
      421 aagaaaaaaa gtgttcccaa aagtttgcag actgggggaa cactcaataa taaagtgctg
      481 agaaaacttt atttatagct attatatcaa aaaagaaggt gaaaaggtgt tttcttctgg
      541 aaatgttgta gttgacaaac ttagagaaat aaattttgac ggaaatgtaa tccacacaca
      601 tggtacgatc aattaagaaa aaaaacgaaa aaaggtgttg agaagcccta tttgaatgca
      661 atagttatct tagcagaaat tacctattgg tatagaccta aaaaaatttt taacgatgat
      721 ggtcagctac ttggttatca aaaaaagttt cttgaagata ttttgcaaaa atcatataag
      781 caattaagca aaaaaacagg attatcagaa cgagctataa aagatgcaat tgtgtactta
      841 gagaaaaagg ggattatcaa aagggttttt agaaatattt attatgagaa aagagcagtt
      901 acattatcaa atgttatgta cattgtttta aatgtacata aattgataga aattactttt
      961 cctactttgg attgcgaaaa aaaggagtat atgccgaaaa ttcgtcagag gggtatcgaa
     1021 aaaaagagat atgtctcttt aaaaaaaggt agaggtatgt acaaatttgt atatacgtat
     1081 acaaagacta cttcacagaa catatcaaag actactctcg ttatggaaga agaggatatt
     1141 aatataaaga ggatttacag taagattagt gttctttatg acgcaaataa cgaagatggt
     1201 cgaaaattat ttcaagtgta tcactatttg agaaacgcta atgtagatac agaagatatc
     1261 agaaaaatca ttgaatattt atctgaaaat actcagtatc ttattgctga gtgtattata
     1321 aaacaacatg aagcatgctt ttttcaaaga aaaactgaag aagggttata cgattatgta
     1381 acatattttt tgaatggact tcagaaaaga tataaaagtc aatttttaga aaatgtagag
     1441 cctatagatc agttcaaaaa agctttcgaa atcaaagagt tacctgaaat tactatgcat
     1501 aattggttga aaggagaaga atcatgatta aaaatcgctt aatagatcaa atgtggtgtc
     1561 ttgatcaaaa tacagatact ctcgaattaa tcgagagtat ctgcttcaac acagtagttc
     1621 tcgatttaag agctgaaaat gacaattgtg taactcatat cataattaat caaaaaatgg
     1681 ctcagcagct ctctgagagc ctaagaaaat gggctgaagg agaaaactat gaaagcaatt
     1741 aaagaaaaaa tcgttagtag aaaaataaac tggttagcac tcagtgtact tgttttaatg
     1801 attagcgttg tttttacaag taatattgtt gaagctagca ttttcaattg gtttgatgac
     1861 acaaaaagtc aaacagagtt tttgaataat tctctttata gaccttacct aaaaaaatac
     1921 aatggagatt tccttagtca gttcgggatt tggcttggat gggctgtggt aaaaggcatg
     1981 tttactgtga ctgattcgat tcaaaatatg attccagatg tgttggattt gttcaatttc
     2041 attgaaagta cagggctaaa taatgtttat caatcagtca taaatacaat tgtcgttgga
     2101 ttaatgatcc tttcattgat gtttgttggc tacaagatga ttactggaaa aggaacaata
     2161 gatttaagat ctgttggaat gaatatagtg atgtctgtag cgttaattct tttaatgcca
     2221 acaatgattt ctagcggtat tcaatttgcc aaaatatttt acaatgatgc tactacgatt
     2281 acaaattcag atgacggagt cgcttggtca ttgataaaac aaggagtaac agatctggca
     2341 tatattaata aaacagatca atactcaagc attgataaaa cggaggatcg aaataagtta
     2401 acacgaaaaa attttcaaca gacagattta acacaagtgt tgacggataa agtgattgat
     2461 aaattggaaa aagaaaatcc agctgcggat aatcttcgct atgaattagt tgaaaattct
     2521 aataatgaat ttgttgccac aaagttttct gataacttct tatcaacatt ttctgatagc
     2581 ttgaaatcag gttattatcg atatcaagca aatctttggg ggatttcgat tgggttgaca
     2641 gcgttggcaa tcgcctatgt gttcagcgcg tttgtgatca ttacagcaat attagaactt
     2701 gcatttaaac gtgtgttagg tgttcttgtt tttgcgacag atattgaaac aggacaacgt
     2761 tcaaaagttg ttttatccga tattttacaa tgttacttaa cagttggatt tcaagggttt
     2821 ggactatcta tgtttgctat gtttatcaat tttttaaatg ctggacaagg tatctccaca
     2881 aatattttta taaaaacaat tgcttatatc tgtgctgtgt ttgtgctgat aaaaggcagt
     2941 ggaacagtca tgagatactt tggtgttgat attggactca aagaaggcta tgggcaactt
     3001 gcttctgctt ttggtatggg cgcaatgctg tttagaaaag gatcaactgg atttaatcgt
     3061 gctaaaggaa gcggaaatgg aaatggatca gacagtggag aaggagaaga tcgaaaacca
     3121 gaaaagaatt tcggcgaaac tctatcaaaa aaagctggca aaactggaag agctttggga
     3181 tatgcccatg aacgtggatt atcaggttta gcttcagatg gagctacaat ggctagtgaa
     3241 agagcaataa aaccatttaa gagtatgcgt gatatggcta atgataccaa gaataagttc
     3301 aaagaaggtt tagatgatgg tacagtgtcc gctattaata aaaatagcaa acctatgtta
     3361 gctaaaaata aagaagatga aactggaaaa tatgctgatt ccatgccaac acgtctttcg
     3421 gatagaaaag atggtgctaa ggttgaaaat gccgatagaa ttatgtctag ttctgaacgt
     3481 atgcgtgaag caatgaaaaa taatgctgaa tcgaataata atcctgtaag tgcaattcag
     3541 caaaaagttc aacaagatat tgaagaacgt aaaaatgcta tgcacggtca agctaagtct
     3601 gctgaagaat taattaatca gaagcgacaa gaagctaaat acacgcctga agcaatgaat
     3661 cgtgaggaaa tgttaaggaa acgtgtagaa gggcgtacag gagctaatac ggacgaaaaa
     3721 gaagcgttga ccaaagaaag aatccaagaa gctaaaaact caaataccgg attagaaaaa
     3781 ttggtcaaag aaaatctaca aaattctaca agtagtcaag gtggccgatc agtagatgtc
     3841 cgagaaaata ttcaaggttc attgaatggt catgtcggac ggaatgttga tgtaagagag
     3901 aatttacaaa gctcttcaaa tggtcaaggt actaagacaa ttgatgttcg agaaaatgta
     3961 caaaattcta caagtagtca agggtttaaa tcaatggatg taagagagaa tgtacaaaat
     4021 tctacaagta gtcaagggtt taaatcaatg gatgtaagag agaatgtaca aagttctaca
     4081 agtagtcaag gattcaaaac agtagatgtt cgggagaacg ttcaaaaaga tagtggagaa
     4141 atgaaagaaa aaacacaaaa aatcaatatc gttgaagaca gaaagtcagc gaaaaaattt
     4201 gattcaaatc atgaaacagt gattattgat agtgaaatcc gtgaaaatga taagggatca
     4261 aaaccaagac gaagatttac ctatgaagac aacgagttat tcagagatac acttaatgat
     4321 cctaatccgc ttttcgataa tctattaaaa aaataaaagg gggaattgtc atgaaattaa
     4381 ttctaaagat tagtacgcat gatttcttag aacttgacga attatgtgaa tcagtaaaga
     4441 acgcaacaaa tgaaaaaatc acggtcacta attatattga actgatgatt caagaagaat
     4501 taaagatgaa acatgagata aaaaacaact tgaaatattg ttatctagaa aaaagtgaag
     4561 agataatcca tgaattaaat ggcttttaaa aattgtattt gacatgaatt tacgacaagg
     4621 aattagtggg gattaagcga ttattctctt ccactttaga gaaataatgg gttaaaaagc
     4681 cagaatacgt agagcaatat tgacaaccta taaaggagct attgttgaaa attgaagtaa
     4741 caaaaagata aagggaggtt tacatctatc agattaaaag tgatagtaca cccatgaaac
     4801 ctgcaaaaga gttgaaccta atgtatatcg gtttaactct ttttatttgc aaattaaaaa
     4861 aatatcaccg aagtgacgaa tgaaactatg accaaat
//
LOCUS       DAZBSA010000140         4837 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000140 DAZBSA010000000
VERSION     DAZBSA010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.142"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            59..562
                     /locus_tag="ACHGMT_002634"
     CDS             59..562
                     /locus_tag="ACHGMT_002634"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6836330.1"
                     /translation="MENSIYLVTEPNTQLKDQDNLVSELSRQKQVYKFLELLLNSASY
                     LNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIK
                     EYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLG
                     LGIINGE"
     gene            659..2908
                     /locus_tag="ACHGMT_002635"
     CDS             659..2908
                     /locus_tag="ACHGMT_002635"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFN6836331.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            2911..4086
                     /locus_tag="ACHGMT_002636"
     CDS             2911..4086
                     /locus_tag="ACHGMT_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN6836332.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRVTRGAS"
     gene            complement(4089..>4837)
                     /locus_tag="ACHGMT_002637"
     CDS             complement(4089..>4837)
                     /locus_tag="ACHGMT_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFN6836333.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKL
                     ALKGITISVPPRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVKGLES
                     RFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1664 a          719 c          883 g         1571 t
ORIGIN      
        1 gtaataatat aaacataata aaacaaaaat tatccaaatc taacatagga gggtggatat
       61 ggaaaattct atatatttag taacagaacc taacactcag ctgaaagatc aggacaatct
      121 agtcagtgaa ttgtccagac aaaaacaagt ttataaattt ttagaactcc tcttaaattc
      181 agctagttat ctgaacgaag ggataattac taaaaagatg aatccttttg ttcgtcaaac
      241 aattgaaata atacgtgaga atgcgtatca aaaattatct gttaaagata ttgctgtcag
      301 tctatcgata cacgaaaatt atttatcacg attgtttaag aatgatatgg gaatatcaat
      361 aaaagagtat attagtgaaa tcaaaatttc tattgcaaac gatctgttat catcaactga
      421 atattcaatc cagcaaatca gtgacaagat aggattctca acagtacaat ctttttctaa
      481 agcctttaag aaagcaaatg gtgtttcacc gggagtatat cgaaaacgta gattaggtct
      541 aggaatcatt aatggtgaat aaactaaaca tttgattttg ataaatattc gttactttaa
      601 acgcttacta tttatgtgag cgttttttat aatgcaataa agaaacggag gagagcttat
      661 gtataaggat aatgaaatga atattattta tcgatcagaa aaccaacaat ttcatttgtt
      721 taatcaggag ataagttaca tttttaagat atcggaagat ggtaaattat tgcatttata
      781 ttatggtgag aacttaccag ataatgacta tagtcatcta attgagatgc atcatcggcc
      841 tatgacaacc tatagaaatg aaaacgattt gctatattcg ttagagcact taaaacaaga
      901 atttcctgaa tatggtacaa cagattttag acatcctgct atttcattac tacagaaaaa
      961 tggctctcgc ttatcagatt ttgtctatca agggtacgaa gtggaagaag gaaaacctaa
     1021 actagaaggt ttacctgcta cctatgttga taataatgat gaaagcaaaa cattgaaagt
     1081 ttatatttta gattcagtta caaatgttca agtagaatta ttatatacaa tttatagaga
     1141 ttatcctgtt attactcgta gcgcgcgagt tatgaataaa ggaaaagata ttcaaataat
     1201 cgagaatatt agcagtttat cattggattt acccgatgca aattatgaat ggcaacaatt
     1261 atctggtgct tggggtagag aaagaactat taagtcgcgt actttacaac aaggaattca
     1321 atcaatcgaa tctactcgag gtatttcaag ccatcagcat aacccatttg ttactttaaa
     1381 aagaaaaaat actgatgaaa atcaaggaga agctattggt gcagcactag tttactcagg
     1441 aaacttctta attcaagcag aagtagatac atgggatgtt actagactac aagttggcat
     1501 caatccattt ggatttaatt ggaaactcaa tccaggagaa atatttacag caccagaagc
     1561 gattctggtt tattcaagta agggtttaaa tgccatgagt caaacttttc atcaactatt
     1621 tagaaaacgc ttagctcgtg gtaagtggcg agaaaaagac cgtcctgtct taattaataa
     1681 ttgggaagca acatattttg attttgacga agaaaagttg gtaaatattg ctaaaaaagc
     1741 atttgatgtt ggtattgaat tatttgtatt agatgatggc tggtttggag aaagagaaaa
     1801 cgattgtgca ggattaggag attggcatgt aaatcctaaa agactaccta gaggaatcgg
     1861 cagcctttca gaaaaaattc gtagttttgg tatgaaattt ggtttatggt ttgaacctga
     1921 aatggtgaat aaggatagtg agttatatcg cacacatccg aattggatta tttctacacc
     1981 taatcgaaaa cctaatcatg gtaggaacca atatgtatta aattttgggt tagatgaggt
     2041 tgttgaaaat attttcgatc aaatgtgtaa aatcattgat gaatcaaact tagattacat
     2101 taagtgggat atgaatcgtc cattgactga tgtatttgac agtcacttgt cagcagatca
     2161 acaaggagaa gtattccaca gatatgtact aggcgtatat cgcttatatg aaaaacttat
     2221 aacaaaatat cctaacatac tatttgagtc ttgttcatca ggtggtgggc gctttgatcc
     2281 tggaatgctt cactatgcac ctcaagcttg ggctagtgat gattcagatg caattgaaag
     2341 gttaaaaatt caatatggaa ctagtatgct gtatcctctt tcgtctattg gtgctcatgt
     2401 atcaattgtt ccaaatcatc aaacaaaccg tataacacct ataaaaacta gaggaaatgt
     2461 agcttttttt ggtgcatttg gatatgagct tgacttaaat gaattaagtg aaggcgattt
     2521 gaggattgta aaagaacaaa ttgcctttta taaaaaatat agaaccataa ttcataatgg
     2581 tactttttat agactattat caccattcga tgatgataat caaaccgcgt ggatggttgt
     2641 tagtcatgat aagaaaacgg ctattgttgc tcattataaa acattaaatg aggtaaatgc
     2701 tccttaccgc cgactgaaat taaaagggtt agataatgac ggactttata atgtaggaca
     2761 aaatagtttg cgatctggaa tggaattgat gagagcagga ctagtctgtt cagatgcatc
     2821 tagtggacaa ataaatgatc agaatcgacg cccagaaaca tttgattttg attccaagat
     2881 ttgggttata acacaggagg aaaagtagag atgaataacc aaaaaagctt acttcaaaaa
     2941 ggagcaatct taagtatttc actaatgtta acaagctctc aagcaattaa tggtgttatt
     3001 ccacaaatga aacagtcttt ggggatatca caaagtcaaa gtgaactttt aggaactgca
     3061 ccaagtataa ctgttatttt gtttatctta ttatctagtt actttgccaa aagaatagga
     3121 atgaaaaaga caataatttt aggtttatta ttagctggta taggaggaat tattccagta
     3181 tttacagcta ccttccaaac cgttcttatt agtcgagtaa tattaggtgc cggtttagga
     3241 gtatataatt cattggctgt gacatatatc agctctttat actctggcaa tactagagca
     3301 acgctattag gtattagaaa ttcaatggaa gctttaggtc agacaatcct tatttttctt
     3361 gctggagttt tagtgaacat taattggacg gcttcgtttt tagtctatgc aattgcgttt
     3421 cctgtagctc tcttattcgc gctaaaagtt cctgaaatta gagatgaaaa tagtgacata
     3481 cctgaaaatc atgtaaaaga aaagatgaat ccaatggttt ttgccctagt tctatttgca
     3541 atcttactcg taatgaatag tattgctatt tctgtacgat ttgcatcaat tgcaactgaa
     3601 attaaagggg taaactttaa tgctagtaac tatttagctc taatgcctat cttaggaata
     3661 ctagcaggat ttatgtttgg accaataaat aagtggatag gaaaaggaac cctatacttg
     3721 ggaattatat tctttataat ttcaaatttg ttaattgctg tgtcaaacgg taatatgacg
     3781 ttcctgctaa cgggtttatt tttatcaagt ataacaggat catggtgctt cccatttatc
     3841 tttagtaact tagataaagt cactacaaag aatacaatta attttgctac ttcattgatt
     3901 tttattggct gtaatattgg taattttata gctcctattg ctatgcaatt agcacaattt
     3961 ctaacaagaa gtacaaattt gactgctcca ttttttgtat tcgcaggaat atttattgtg
     4021 atcctatttg taacattttt tgcgacaaat aaaaaaaata ctagagtaac ccgtggtgct
     4081 agttaatttt aatatcctaa agaatatctc aaagttcctt caaaacgtcg ttgtgctcga
     4141 cttaatagaa cactgtaagc cagtaatatg ctttcaaatc tactctctag ccctttaaca
     4201 gaacgtgagt tgaaattttg acaacctaat ttttctaagg aagaaaaaac agtctcaact
     4261 gtttttcttt gttttccaag taagctgtgg tctagttttt ctgatttatc catgttttta
     4321 cgtggcggaa cggatatagt aatgccctta agggcaagct tttcatggat tttatggcta
     4381 atatatcctt tgtcccctag gatattggga agattcgctt cttccgataa agtctctaac
     4441 actttcacgt catggacacc tggattcgtg attgagtaag taatcggaaa gccagtctta
     4501 gtaacgatca tatgaatttt aagaccataa aaataagatt tttttgttga gttatagcca
     4561 actttggcta tttgattcaa tagttttgct tgtctatttc taactggttt acataatgga
     4621 ctaggaaaac tatctataat tccgactagt tcacctttgg tgagcttctt gatgaaaaag
     4681 tatcgaataa tcttgatagt gtaagctaag tttgaactta agcgagtgaa tcgactccta
     4741 ctaggaaagt caccattagg aaataagacg gaacaaaccg ctctatacgt ggctctttgg
     4801 ctagtatagc cattaattat gccccatata acacaag
//
LOCUS       DAZBSA010000141         4771 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000141 DAZBSA010000000
VERSION     DAZBSA010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.143"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            52..723
                     /locus_tag="ACHGMT_002638"
     CDS             52..723
                     /locus_tag="ACHGMT_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836334.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            782..1222
                     /locus_tag="ACHGMT_002639"
     CDS             782..1222
                     /locus_tag="ACHGMT_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836335.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1234..1902
                     /locus_tag="ACHGMT_002640"
     CDS             1234..1902
                     /locus_tag="ACHGMT_002640"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFN6836336.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1966..2589
                     /locus_tag="ACHGMT_002641"
     CDS             1966..2589
                     /locus_tag="ACHGMT_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFN6836337.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2645..3031
                     /locus_tag="ACHGMT_002642"
     CDS             2645..3031
                     /locus_tag="ACHGMT_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFN6836338.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3244..4635
                     /locus_tag="ACHGMT_002643"
     CDS             3244..4635
                     /locus_tag="ACHGMT_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFN6836339.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1589 a          784 c         1012 g         1386 t
ORIGIN      
        1 tttcttttca aaatatgcta ttttaaaaat gatgaaaaaa gggggagaaa aatgaatttt
       61 ttagttgttt taaaagatac tctgattgag cgatttacat ttctatatgt tcaattgcag
      121 gaaaataaaa cattgctgca tttttcacca gagttccatc tagaggggct aacattagga
      181 gaaatctatc aagcagaagg gctgtcggct gttttgcact tttttaaggt agaattggaa
      241 ttaccagtaa aagattatat cgagctttct cttgaatctt gggatcgagc agtggttgaa
      301 ctgtttccag aaggtttaat gatcgataca aacgatggga agatccatct gactgcagca
      361 gaactgcaaa aacaaatgcg ttatcaaata gatgacgaaa attctttttc tatttttaga
      421 cgtcagcaaa agattctaaa aagcttttta aaagtgatca gtaaaaaacg gaatctgatc
      481 aaaacgacac agctgttgaa aaaatatcca ggtctgcttc acacgtctat tcaatttcct
      541 caattgatta cgatgatcca tcgttttata cgtatgcaac agctaccatc taagaagctg
      601 tttcttccat tgacggacac atttcgagta gtcaacagag aagataaaaa gaaagtgatt
      661 gtgattgact ttaccagaaa tcggaatgtc cttcaccaag ttgcaatgga aaaggccaat
      721 taaggctttt tctttttgga tttttttgtc ataataaata ttagatgatg gaggagataa
      781 tatgatgcaa acatggttag aaaggctaat acgatttcag cagaactatc acaaacttat
      841 gcagggaaga tatgcgaaat ttgatcagtt gaatcgcggt ctattgatcg ctgcgcttgt
      901 ctttagtttg ctaagtcgtt ggttgcccta tagaatcgga caattgttgt tcatcgtctt
      961 tttcggatgg atgatatttc gtttcctatc aaaaaaaatc taccccagac taaatgagaa
     1021 tcaacgttat ttgaaatatc ttgatagagt caaagaaaaa tatcatcaga taaaaaacaa
     1081 aaagcaagtc aaaatggatc gaaaaagtta tacttttttt gagtgcccga attgtcatca
     1141 aaagcagcgt gcacctaaag gaaaaggacg aatacgtgtg acatgcaaaa catgtggtat
     1201 aaaattcgaa acgaatgttt aagggagaaa agaatgaaat tagtattatt acgtcatggc
     1261 gaaagcgaag caaattttga aaattactgg acaggatggc tagatgtcgc cttaacagaa
     1321 aaaggaaaag aacaagcagc agttgccggt caaaaaatgc ttgaagccgg acttcaattc
     1381 gatgccgttt atacctctgt tttgaaacgt gcgattctta ccggacaagt cgcactagaa
     1441 gaaatgggtc agttatggct tcctataata aaaacatggc gtctaaatga acggcattat
     1501 ggggatcttg tgggtaaaaa caaagataag atgaaagaaa tatacggaaa agatcaagta
     1561 aaaacatggc gcagaggata tacggaagtt ccgccgttaa ccgcagaaaa tagatttgac
     1621 cgcagatatg atcacttgga tccgcatttg atcccatacg gagaaagtct ggaaatgaca
     1681 gtgaatcgga tcattccctt atggcaagat catctagcac caaaactaaa agatcggcaa
     1741 gatttactag ttgtgggtca tggtaatagc atccgagcac tgacaaaata tttagaagat
     1801 gttcctgaag atcaaatgga tacgatcgat atcccaaatg cacaaccaat ccagtataca
     1861 ttagcagatg atttgacaat tctggacaaa aaaatacttt aaagaacacc tttatctaaa
     1921 agtgagagat agaaaaaaga agcaaaggaa tggaacaaaa ggaacatgaa aaattatata
     1981 tgggactttg atggtacatt gtttgatacg tatcccgcaa tggtagatgg tgcatggcaa
     2041 gcactaaaag attttggtat cagtatggac aaaaaagaaa tctacttcaa aatgaaaaag
     2101 tattctacta gttatttgat caatgaatca aacttgaatg ccagagagtt taatgaatta
     2161 tttcaccgat atgaaaaaga aagtacggaa gtttcccgtc catttccaga aacgaaacaa
     2221 gtactggaaa tgctaaaaga caacggcggt cgtcatttca tcctgaccca ccgtttgaca
     2281 gaatcaacat ggggcctgtt aaaagaacat cgattggcac atctgattga ggaagtagta
     2341 gggatcgatc aggactttcc tcgtaaacct gatccagcat ctttaaatta tttgatcgat
     2401 acttttcatt tagaaagaac agacacgatg atgatcggcg atcgtcgttt agatatcgaa
     2461 gcagggaaaa atgcaggagt cgctacttgt ctttacgaca tcgatcattt tctgggtgag
     2521 atcccggcag attatgtggt aggaaatctg aatgaaatcc ttaccttgca gagcatcatt
     2581 aaaaagtagt atcccctttc aaaggagata tgttataatt ttcataaagg agtgataaaa
     2641 ttatatggaa atcagaagac aaaaagaagt tgtagaagca tttcattatg atatgagatt
     2701 gccgaaccaa gaagtagaaa cagatttgaa agtaggcttc tcgcctttaa aagaaacgat
     2761 cgaaaactat ccgaaagaac atagcatcgt aggagcacgc ttagagttcc gtttagtgtt
     2821 tgatacatat gttttatctg gaagtgtgag tcagatcaat catatcctca atcggaaaat
     2881 tgaaggtcaa aatgatgtca cacaagaaga agtggacgag ctagtagcac cgttattcga
     2941 tatcgtacgc cgaatgtctt atgaggtgac agagatcgcc ttagatcagc ctggggtaaa
     3001 attgaatttt caatcagaaa aacaagaata gacagtaaaa acatacctgc gatagattaa
     3061 agctatcaca ggtatgtttt tttattacta attgtaagca aaaagaatgt catttcgata
     3121 aaaatcgtct aaaatataaa agtcactgaa aaaaattttt tcaggaggat ggttgaagaa
     3181 tcattctcgg gaacctttac ttccttcgat ggacttgtca ttaataatat gaggaggaga
     3241 aaaatgacaa cactcatcac agttttagtg gttctagttt ttgcggcatt attatatgtg
     3301 ttctatcgta tgcaaaagcg ccatacaaaa ttttcaacac gcgtctttac agcattagga
     3361 gttggtatcg tctttggcgg tctgcttcaa ttgatctttg gtgtagacga caaagtcgtt
     3421 actcaatcaa tggaatggat cgggattgta ggtcaaggat atgtatcatt cttgcaaatg
     3481 ctagttatgc ctttagtgtt tatttcgatc attggtgcat tcacaaaaat gaaagtttct
     3541 gaaaagctag ggaaaattag tgcgactgta ttgacttctt tacttggaac aacagctatt
     3601 gcagcattga tcggaattgg ttcaacaatg ctgttcggac tccaaggagc taaattcaca
     3661 caaggagctg cagaaacagc acgaatctct gaactggcaa cacgtcagga aacagttgaa
     3721 aacttatcga ttccacagca gattttatcc tttatcccta aaaacgtttt tgctgattta
     3781 gcaggtagtc gtgcaacaag tacgattgga gtcgttattt tcgcggcatt tgtcggaatc
     3841 gcttatctag gcgtgaaaag aaaagcgcca aaagaagcag cattcttttc agatttagtc
     3901 gacagtctgt ataaaatcat tatgcggatc gtgacgcttg ttttgcgttt gacaccttat
     3961 ggagtatttg ctttgatgac aaacgttttg gcaacaagtg attttgcagc tatcgtcaat
     4021 ttaggtaaat ttgtcgttgc ttcttacgta gcattgcttg ctgttttgat tgtccactca
     4081 ctgattttga ttggtgcaaa agtcaatcca atcaactatt tcaaaaaagc ctttccggta
     4141 ttgagctttg cgtttacttc acgttcaagt gctggagctt tgccattgaa tatcgagact
     4201 cagacaaaag ctttaggagt agatgatgca acagctaatt tctcagcaag ttttggtttg
     4261 tctattggac aaaacggctg tgctggcgtt tatccagcta tgttggcaac gatcgtcgca
     4321 ccagcagctg gagtgaatgt ctttagtttg gaatttattt tgatgcttgt ggcaattgta
     4381 acaatcagtt cttttggtgt agctggagtc ggtgggggcg caacatttgc ttcactgatc
     4441 gttttagggg caatgaactt gccagtagca atcgttgggt tagttatctc tgttgaacca
     4501 ttaatcgata tggcacgtac agcagtaaac gtcagcggta gcatggtagc tggtatcgtg
     4561 acaagtgcac gcatcaaaga tttagatcgc gatgtcatca atgatgattc taaagtgatc
     4621 gatgtgaatg catagtcaaa aagaggttgt gatagaagtg gtcagcttta agaaataaga
     4681 agaagtttcc taaaaatagt ttctcaaatt tttgtgaaat ttcggcttat ttccgagaag
     4741 ctacttctga aacaccgtga ataagaggtt g
//
LOCUS       DAZBSA010000142         4754 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000142 DAZBSA010000000
VERSION     DAZBSA010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.144"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(185..1147)
                     /locus_tag="ACHGMT_002644"
     CDS             complement(185..1147)
                     /locus_tag="ACHGMT_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN6836340.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1149..2588)
                     /locus_tag="ACHGMT_002645"
     CDS             complement(1149..2588)
                     /locus_tag="ACHGMT_002645"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFN6836341.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2773..4719
                     /locus_tag="ACHGMT_002646"
     CDS             2773..4719
                     /locus_tag="ACHGMT_002646"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFN6836342.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1508 a          812 c          788 g         1646 t
ORIGIN      
        1 gtaacagttg tgtaaaaata tcattttaga gcatgaaaaa aggagtggat ttctccactc
       61 caaaaaagtc taacttttgg gggtcactac aagttgacaa ctcctttttg tttacaaatc
      121 aattggatat ctacaaattg tattttctaa cgaattttat agacattaaa aaattaattt
      181 aacgttaaat acttcctcca gaaagtattg agattggtaa aatataatcc ttatttgtct
      241 tttcgccttt aatttttttc agaagaatat caacaataag acttgcaatt tcatcaattg
      301 gttgtcttat tgttgtcaat tgtgggaaaa atttctcaat aaaagaggta ccatcgtatc
      361 caataatttt taaatcttct gggatattaa gtttaagttg atgagcaatt ttcatagtga
      421 gaatggctgt taaatcatca gaaacaaaaa taccatctgg tttgtgattt ataatagttg
      481 actttatttc catttcacga cggatagtag acagattatt aggaattttg aaaatttttc
      541 catttggatt ttgaaaagaa aaacctagtg cacgtaattc tgtgggagaa tccgtgttat
      601 catttcctgt aatcataatg gtattttgac aaccattttt ttgtaaagtc ttagctgcaa
      661 gttttccccc ttcaaagtta tctgaagaaa caatggaaac attaggagct aaatttctat
      721 caaaagcaac aataggtgct ctcacacgct cataatcagc aattccaaga ttatggcttg
      781 atgagattat gccatcaact tggttggctt caagcatttc aatgtattct ctctctttag
      841 caggatcatt ttgactattg caaataatcg ttttatatcc atgtttaaat aactctattt
      901 cgagatactc aatgagttca gagtaaaaga tattactaat attaggaaaa ataagaccaa
      961 taagctgagg tgactttcct tgaagtccac gtgctaaatt atttggttta taaccaagag
     1021 ttttcatagc ttcatgaact ttttgttttg ttttttctga taaatatccc ttattattaa
     1081 tgacgcgtga aacggttgtt gggctaactc cagctaattc agcgacatca gttaattttg
     1141 ctaccatttt aatattttaa ttcaaagtat tttccagtag gttcgcctga tttgatttga
     1201 ataccatttt ggtgattttc agggaataca cgactagtaa acactttttc tcctttatta
     1261 ataaaaatct caatgattga gttatctaca aaaatgttag cagttgtttc tttagtatca
     1321 attgagcatt cacgaatcgt accaaagtca gtggcatatt gaatgcctgc tttgctgcga
     1381 tctaaaataa tttttccttc tttggtatct acaatcaagc taagtccatt tcctttacta
     1441 tcagagaaaa gaagtaactc cgatttctga tttggaggaa aagttagttc aagctcataa
     1501 gtattatttg tttctgcttt tgctgtaaat tgttcagaac tagcacgtaa tgatgtaata
     1561 gcttctactg gatattggta aagtttacca tcttttattg aaagttcttt tacaagactc
     1621 attgctccct gataatcata cttatcagtt ggataatcga tatcaggtag tccaatccaa
     1681 ctgacaatca gagcacgacc atcaggagta ttgaatcctt gagtcgcata cgcttcaaat
     1741 ccataatcaa gattttgaat ttctgaagcg cccacaagtt ttactttttc tatgtcaagc
     1801 gattgacaga tcttaaaagt attcggatag atattcccat aatttagctc agatttatcc
     1861 aacccttgag gacaataaat gagaacaggt tttttgtcta caaatactag atttggacac
     1921 tcaatcatgt attctgaacc agtgccgcca aaatcaaggt tgccaacttc ctcccatgtt
     1981 tccacgttat tgtcatccgc tttgtaaagt ttgataaagc ctgatttttc taaattttgt
     2041 gctccaacaa tcgcatacaa ttgtccttca taattaaaca tttgtggatc acgaaaatga
     2101 tcagttacat cagcaggttt atgaataaga acattctcac ctttcgtaat gttgtagtct
     2161 ttatccatcc atgctccgat ttgaagtgta tcacgttccc aattttcatt tcgaacgttt
     2221 cctgtataga gtaagaatag attcccctct atttcatatg cagaaccgga ataagcgccg
     2281 tggctatctc gttttgtgtc tggaagaagt tctgctcctg ttacatgaaa atgaactaaa
     2341 tcatctgatt cattgtgtac ccattctttc aacccatgag cagctccaaa tggccaattt
     2401 tgataaaaaa gcgtatattt tccgttaaaa aaagaaaaac cattaggatc gtttaacaat
     2461 cctgttttag gttcgatatg atagctagca cgccaaggtg atcgttttac attatcctta
     2521 attttatcca tctcatcttt agtccaatca gaataagcac gatatcgtat ttctcgaggt
     2581 aagttcatat tttactcctt caatttatct ttttacaact agtatggtaa acgttttccg
     2641 ttgaaaaatc aatcatttca tttaaaaaaa taaactatat gacaaacgtt tgacatattt
     2701 ttaagatatg ctaaaataaa tttgcatttc agtgaaccgt tttcacgtaa aaataaaaaa
     2761 ggagtgattc gaatgaatta cagtaaagta gcttccgacg taatacaagc ggtcggaaaa
     2821 gataacctta ttgcagctgc acactgcgct acacgtcttc gtctcgtact aaaagatgac
     2881 acgaaagtga atcaaaaagc tttagacgaa aatccagatg tgaaaggcac atttaaaatt
     2941 gatggacaat atcaagtgat tatcggtgca ggtgatgtta atttcgttta tgacgaactt
     3001 atcaaaaaaa ctggactttc ggaaatttca acagatgatt taaaacaaat tgctagtaaa
     3061 gataaaaaat tcaaccccat tatggcatta attaaattat tatcagatat ttttgttcca
     3121 attatacctg cccttgttgc aggtggttta ttaatggcat tacaaaactt tttaacagct
     3181 gctggacttt ttggaccaaa atcaattgaa gaaatgtatc ctgcgattga aggtatttcc
     3241 tcaatgatcc aactgatgtc cgctgcacca ttcatgttct taccaatttt agtaggtatt
     3301 tcagctgcta aacgttttgg agcaaaccaa tttcttggtg ctgcaattgg tatgatcatg
     3361 acaacaccag atcttggtgg ctcaactgaa tattggaata tttttggtta tcatgtttca
     3421 caaacaaact atgcttatca agttattcca gttttagcag ctgtttggat attatctata
     3481 ttagaaaaat tctttcataa aaaattgcca tcatctattg actttacatt cacgccatta
     3541 ttatcagtaa tgattactgg atttttgact ttcacagtta ttggccctgt aatgttattg
     3601 ttgtctaatg gaattacaga cattattgtt tggctatata acactactgg atttattgga
     3661 atgggtattt ttggaggaac atattcactt atagtcatga ctggtttaca tcaatcattt
     3721 ccagctattg aaactcaatt actttcagcg tggacaaatg gtatcggaca tggagacttt
     3781 atctttgtag ttgcatctat ggcaaacgtt gctcaaggtg ctgcaacatt tgccatttgg
     3841 ttcttaacta aaaattctaa gacaaaaagt cttgcttcct ctgcgggtgt ctcagcactt
     3901 cttggaatta cagagcctgc cttgttcgga gttaacttaa aataccgctt tccattcttt
     3961 tgtgctttaa ttggttcagg aattgctaca gcagtagcag gattacttaa agtagttgct
     4021 gtttcacttg gttcagcagg tttcttggga tttctttcaa ttaatgcaac atctattcca
     4081 ttttacgtat tgtgtgaatt gattagcttt gtagttgctt ttgcaattac ttacttctac
     4141 ggtaaaacaa aagctgctgg tatctttaaa gcagaagaaa ttgctgaaca aacaccagtt
     4201 aattcttcaa aagtagcaac aacagatcca atatctactg atgttgaatc aacacaaaca
     4261 aaagtttcat cagaaacaat tgttagtccc atttctggta aagtgattag tctttcttca
     4321 gtaaatgatc cagtcttttc atcagaagct atgggaaaag ggattgccat taaaccaaat
     4381 ggaaacacgg tccattctcc agtagatggt actattcaac tcacgtttga aactggacat
     4441 gcttatggta ttaaatcaaa taatggtgct gaagttctaa ttcatattgg tattgatact
     4501 gtatcaatgg gaggaaaagg atttacacaa aaagttacta ctaatcaaaa agttagaaaa
     4561 ggcgatattc ttggagcatt tgaccgttca atgattgaga aagaaggtct tgatgataca
     4621 acaatgatta ttgtgaccaa tacttcggat tattcagaag taaatatagt agctgaaaat
     4681 acagtcactg agggtactga tttacttgaa atcaaataaa tatatttccc tgagtaaaga
     4741 tggaattttc tttc
//
LOCUS       DAZBSA010000143         4740 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000143 DAZBSA010000000
VERSION     DAZBSA010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.145"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(82..1890)
                     /gene="pepF"
                     /locus_tag="ACHGMT_002647"
     CDS             complement(82..1890)
                     /gene="pepF"
                     /locus_tag="ACHGMT_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFN6836343.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2066..3064)
                     /locus_tag="ACHGMT_002648"
     CDS             complement(2066..3064)
                     /locus_tag="ACHGMT_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFN6836344.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3290..3943)
                     /locus_tag="ACHGMT_002649"
     CDS             complement(3290..3943)
                     /locus_tag="ACHGMT_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFN6836345.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="ACHGMT_002650"
     CDS             complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="ACHGMT_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFN6836346.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1390 a          999 c          742 g         1609 t
ORIGIN      
        1 taaaaaaata cgtcgactcc cttacaagag agccgacgta tttttttcgt ttctattcga
       61 gaaaatcaaa aatagaaagc cttataaacg atcgatcaat tcttctaatt catttagacg
      121 ttgttcaaac atcgacattg catcttcaat atatgcagcc tgtgtcatat caactcctgc
      181 ttttttcatt acttccacag gataatcgct gttacctgct tttaggtaag ccaagtagtt
      241 ctctagtgcc tctggttcct ggtttagtat tttcttcgcc agtgctgaag cagcagaaaa
      301 gccagtcgag tattggaaaa catagtaatt ataataaaaa tgcggtatcc gtgaccattc
      361 gaatttaatt tctgggtctt cttcgactgc tggaccataa tattttgcat tcaatttacc
      421 ataactatca cttaggtatt cactagtcaa tggcacacct ttttcatctt ccgtatgcat
      481 gaaatgctcg aattcagcga attgtgtctg gcggaaaact gttcctttaa acccatccaa
      541 gtaatggttg agtacataag cccgtacacg agggtctttt tctgtttcca ataaatactc
      601 cgttaggata ttttcattcg ttgtcgaagc aatttctgct aaaaagatgg agtagtcgcc
      661 gtacacataa ggctggttcg aacgagtgaa ataactatga acactatgtc ccatttcgtg
      721 gacaagcgta aatagctgat ccagcgtatc atgccaattc aataaaatat atggatttgt
      781 gtcatagctt cccgaagaat aagcaccgct tcgtttccct ttattttcga caacatcgat
      841 ccaacgttca gagaatgctt tttctacgat ggccatgtat tcttcaccca ttggtttcag
      901 tgcttctaaa gctttttctt ttgcttcttc gtacgtaaag gtaattggag cttcccctaa
      961 gaccggtgta taaagatcat acatgtgcag tttttccact tctaataaac gtttccgtaa
     1021 ttccatgtat cgatgtaaca aaggcaaatg cttgtttacc acgtctacca aagtatcgta
     1081 tacactttca ggaatatgat tattgctcaa agacgcttct ctggcagagc tgtaattacg
     1141 gactttcgct ttaaaattat gtccttttat atgtgtgcct aaagtagaag caaatgtatt
     1201 tctaaattgt tcgtaaacac tgtacaaccc cttaaatgct gcttcgcgca cccttcgatc
     1261 cgtgctttct agcaactgac catacacgcc atgagataat tggactattt caccattttc
     1321 tccttcaatc gttggaaaaa ctagatctgc attattcaaa acagcaaatg tatcacttga
     1381 agcatcaaag atttcacctg ctccagcaag aagagattcc tgctcagcag ataagacatg
     1441 ggctcgatta tctactattt gctggatata atggcgatag acttccaatt ttggttcttc
     1501 tttgaaatat tgccaaatct ggtcatctga caattgcaat atttctggtt caaaccagga
     1561 aacggcttca ctaactttcg aaaacagact gcttgctctt gcataaagcg cttggtaatc
     1621 tgtatttcca gtgtcttgat cgtttttaag gtgcgcatat acataaatga cttcagtttg
     1681 gcgataaacc ctcaatacga attcaatcgc atttaaaaat tgagaagcgc cttgatcaag
     1741 tgttcctttg tgtttttcag attgttttaa ctcttctgat aattccaaat atttctcatc
     1801 aaactcttgg tcgcttgaaa agatcttggt caagtcccaa gttaaatttt caggcaattc
     1861 ttctcgtttt ggcaactgct ttacttccat gaaatccact ccttttcttc ccttagctct
     1921 atctatcaaa atagatcata gtctaacatc cacatgtcac agcatctaac ttttctactc
     1981 attcatgtgt ctgcttccat caattttagc ataactaaat cttgttttac atagaaaacc
     2041 gattcctagc ctatttttat acttactatt ttttcaaaga agacaagtgt atgcattcgt
     2101 taaaaaactg ctgatagacc atctcttcat caatcatagg aaaaagccat tcccttttat
     2161 gttcttttat ttggcaaagc catgagttga aatcaggaac tttctggttt tccaaggctt
     2221 cttcgtacaa cattcgataa agaaaaacct gttcttgtaa ataaaagaaa aaccgacttt
     2281 tttgatagat ccacgatgat agatgcaaaa gatgtttatg acgcaagtag cattgttctt
     2341 gtatggatac tgtattcttt tgcttgtgaa taagttggcg tgtcagccat ttcttcccat
     2401 cttcactcac tttttctatt tttttcattt ggttcatttt aatttcgtta ctagtgaata
     2461 tttctttaag gtccctacta gaaaaagtcc agcagctact gcaaaaggaa atatgccctg
     2521 atacagtctc tgtaatatga tgatacaaat aaagcttttg tttcttgaga tcagctttcc
     2581 agcaatgcac tcctcttttt ctattatagt agcagcaact tttttctatc aatgagaatt
     2641 gcgattgtcc aagaaaagaa ttaccaagta tccaccatac ttggtaactg ttattcaaat
     2701 aattttgggt tctttcaata aatctctggt gagacagtct agaacattgg atttctattg
     2761 ctagattcgc caacaaaata tccggccttt gccgcaacct cggcaaatag gcttccaaaa
     2821 atactggttc tactgactgt cccagccaat ccatgaagca ttcctttaac tgtaagtgct
     2881 cttttgattc tctttcacta aagaccgaac agttatctga tttttgatgg gcaaaatgag
     2941 cacatttttt ctttcccttt ttcaaaatca ctctttttcg acagccacga caataatatt
     3001 tctttccttt ttctgcatgt ttagcaaaac agcacttatt atattcattc attgcaagca
     3061 gcatttttct ccctctgaac ttatttacgt ctaagaattt atttacacga aaaaagaggg
     3121 cgtgaaaaag ctattctgca tcaagtaata agaacagcta aacaaaaata gttcccaata
     3181 tttttcgttt ggttgggctt gttaccgcag atgcaagctt ttgaacacat ttatacgaaa
     3241 aaaagactgc gacacaattt ttgtcacagt ctcttactat aatgctgatt tatacaaaat
     3301 aaaatttcgt tcgctcgata gcattacgcg ccatcagaca ttgtccatat tcattcaatg
     3361 tttctggtgt acgatcgctc ttttcagcaa actccaaaat tcgagcaatc tgattctcta
     3421 catccgcttg gttgagattt tccatccaga agtgaacgac taggtaatag cgctcttcga
     3481 ataaatacaa atcagtccaa gcagactcta gttcgacatc tgttgcaagt tggatcactt
     3541 gttcaaaatc ttcaaaagca aaagttgctt gtctttcctg ttcttctagg gcgcttccag
     3601 ataattcttc ctccggatcg ctgccaataa tctgctgacg aagaaagtca ctcacatctt
     3661 ctgaatcaac agtagaatct tctgattgag aaatttcttc atccggaaga tttttactga
     3721 taaacagttc taatccgtct ccttttggca aaacttggaa agtgacagct tcgcttcctc
     3781 ggaactcctc ttcgatatca acttcttcca aaatactata aaagaaattt tcgatttctt
     3841 tgtgattgcc aagtaaatcc aaaaatgaaa cgccccgttc ctctaaatct tcatgaccaa
     3901 ttagtacacg gatcgtattt tcattaatat gttccatttc cattttgcta cacctcactt
     3961 tgccacaatg tttaatatag attcattgta gcgaaatcag cttaaatgta aagaaaaaaa
     4021 atctcgctgt taagcgaatt taataaaacc agcggatttt tcaacctttc aagctatctt
     4081 atttaactat aacatttttg gcagacgaat actatatagc cgacttttca gctaattttc
     4141 cctctctatt tttcgtgcaa tcatcatctt ctactacctt tttatacatc taatcaagaa
     4201 tactttttgt aaaaacaaaa aaacttggaa aaactatctt tgtaatagtc ttttccaagt
     4261 ttcgtattat ccttctataa tccagccatc aattgtgctt gacgtaattc taattgtcgt
     4321 acatcgcgcg gtaggaaccg acgaatttca tcttcattaa aaccaacttg cagacgttta
     4381 tcatcaatca tgattggacg gcgaagcaat ccaggattgt tttgcacaag ttccaataat
     4441 tcttgtaacg gcagatcatc taaatccatg ttcagttttt ggaatacttt ggaacgtgtc
     4501 gagatgattt cttctgttcc gtcttctgtc atttgtaaaa tcgcttttaa ttcagaactg
     4561 ttcaatggtt ctgaaaaaat attacgttca acaaatggaa tctgatgttc ttgcaaccac
     4621 gcacgagcct ttctgcagga agtacaactt ggggaagtat ataatgttaa caacgtgcac
     4681 cactcctttt cgtgataaga atcacttttt tgtaacagaa gatcataata atctcccatc
//
LOCUS       DAZBSA010000144         4700 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000144 DAZBSA010000000
VERSION     DAZBSA010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.147"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(220..972)
                     /locus_tag="ACHGMT_002651"
     CDS             complement(220..972)
                     /locus_tag="ACHGMT_002651"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFN6836347.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1085..2005
                     /locus_tag="ACHGMT_002652"
     CDS             1085..2005
                     /locus_tag="ACHGMT_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN6836348.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2078..3013
                     /gene="murB"
                     /locus_tag="ACHGMT_002653"
     CDS             2078..3013
                     /gene="murB"
                     /locus_tag="ACHGMT_002653"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFN6836349.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3311..>4700
                     /locus_tag="ACHGMT_002654"
     CDS             3311..>4700
                     /locus_tag="ACHGMT_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6836350.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVSICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIW"
BASE COUNT         1648 a          756 c          910 g         1386 t
ORIGIN      
        1 atctgcggta ataagcccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt
       61 cttattactt gatgcaggac agctttttca caatctctcg ataaacagtg ttcaaaagcc
      121 agtttctcgg tattatgcca aatttatgca aaatgtggaa agccattttc acaaaatttt
      181 tcttaatact cgaaactaaa cgactttttc acaacctctt tatttcaaat ctaattctac
      241 tggacaatga tcactgccca taatgtccgt atggatcttt gcatcagcta gtcctggctc
      301 taaatctttg ctcgtcaaaa agtaatcgat ccgccaacca gcattgtttt tccttgaatt
      361 gaatcggtag ttccaccaag agtagacacc ttcctgggtt ggataaaaat aacggaatgt
      421 atcgataaac ccgttatcca acagtcgaga aagagccgct ctttcttctg gagtgaatcc
      481 agcatttttt tgattcgttt tccagttttt caaatcaatc ttttgatgag cgacatttag
      541 atccccacac acgatgactg gtttttgttt ttttagattt tccaaataat tataaaaggc
      601 ttcttcccat tctaatcggt aatctaaacg ttttaattcg ctttgagaat ttggcgtgta
      661 acaggttaca agaaagaaat cggaatattc caaagtaatc agacggcctt ctgtatcatg
      721 gatatcgata cccattccgt aagtggcatt caatgctggt tttttagcaa aaatcgctgt
      781 gccggaatag ccttttcgtt ccgcgtaatt ccaatattga taataacctg gaagatctaa
      841 atcaatttgt cctgcttgga gtttcgtttc ctgcagacag aaaaaatcgg cgtctaactc
      901 atgaaaaact tctaaaaaat ttttatttac gatggcacga agcccgttta cgttccaaga
      961 gataaatttc aataaaagca cactccttcg aattaatttc cttacctttg cgatactatt
     1021 gtacctcatt ttagaaaaaa atggtgtaag atgaaagtaa ctaaatgatc caggaggaaa
     1081 gtagatgaag attggtgtaa tcggtgtagg gaatatcgca gaaaaagcat atttaccaac
     1141 ttatgcgaaa aaacaaggaa cacttgattt ttattttgca acaagaaata aagaaacaaa
     1201 agaaagaatt caaaaagagt acgggttcca acatctatat gaaacgatcg atgagctttt
     1261 agaagaaaaa atcgaagcat gtatgatcca cgcagcaaca aaagtacatt atgaattagc
     1321 caaaaaatgt ctagaaaatg gtatccatgt ttatattgac aaaccactga gtgtccatct
     1381 aaatgaagtt cgtgaattgc aagaattagc tgataagaac caaataattt tgatgatcgg
     1441 tttcaaccgg cgctttgctc ctatggtaga agaattgaaa cgtatcccag ataagcggtt
     1501 gatccacctg caaaaaaatc gaatagcggc aaaagaaacg acagagttcg tcctttatga
     1561 tttgttttta catcttattg atacagctgt gtatttatta gatgaaccca ttttgagaac
     1621 aaaataccag ataagagaaa caaaggaatt tttggaatat gccattttac aattagaaac
     1681 acaaaatcaa acggctattt taacgatgga tttagcaagt ggcgctaata tagaaaaata
     1741 tcaagtaaca agtaaaaatg gtacttatga agtaaacgat ctgacccagc ttgtcattca
     1801 agatgctgat ggaaaaaaag tgaaagaatt tggcgactgg acaaacacgc tagtcaaacg
     1861 cgggtttgag ccaatggtag aggccttctt ttctcagatc cgtttaggta aatcggaaac
     1921 agaattgctt aaacaaaaag aagttgtaag aagtcatgaa ttatgtgagg aagtgcttaa
     1981 agatcactac cgtcatcaac tttgaacaag actggcaaga ttgtattaag cgtgatagaa
     2041 taatgaggac gtttgagaaa agaaaggaaa atcctcagtg aataaagaag aaatagttga
     2101 aagcttagcc acgattacta tattgtttga tgaaccattg atgaactata cgtttacgaa
     2161 aacaggtgga ccagctgatg tccttgcatt cccgaaaaaa caagaagaag taaagcaaat
     2221 catcgattat tgccgaatcc atgatatacc ttggatggta ttaggaaatg caagcaatct
     2281 gatcgttcaa gatggcggga ttcgcggtgt agtcatcatg ctgactgaaa tgaaacagat
     2341 tcatgtcaaa ggaacgatgg tcattgcgga agcaggtgcc tcactgattg atacgactta
     2401 tgcagctttg gcagaatcat tgactggatt tgaatttgct tgcggcatcc cgggaagtgt
     2461 cggtggagcg gtttatatga atgcaggtgc gtatggcgga gagatcaaag atgtttttgc
     2521 tgaagtggat cttttgcttg aagatggaac attgaaaact ttgacaaaag acgaaatggt
     2581 attttcttat cgtcacagta aagtccaaga attacgtgct atcgtattgg aagctcgatt
     2641 cagtttgcaa acaggggatt atgaagcaat caaagcaaga atggatgagt tgaccgaatt
     2701 gcgtcaatca aaacagccat tagaataccc ttcatgcgga agtgtcttta agcgcccagt
     2761 aggacactat acaggtcagc tgatccaaca agcaggattg caaggcctga aatggggcgg
     2821 tgcacaagtc tctgaaaaac atgctggatt catcgtcaat atcgatcatg cgacagcgac
     2881 agattatatt gaattgattg cgcacatcca agaagtaatc aaagaaaaat ttgatgtgtc
     2941 tttagaaaca gaagtgcgga tcattggaga aaaacaagca atatcagaaa caagcagaag
     3001 tttagccaat taataagtaa tggaacttaa aataagtaaa agagcaggac ggtggccgtc
     3061 ctgctctttt ttgtggttgt ttacgcaaat ggcgcaaatc ttgtacattt taacacaata
     3121 aaacaaaaaa tatagaatat ttcctcataa atttttccat tttttgcaat attctcaaaa
     3181 aaaagataaa aaatgagaat aactgatgaa aaaacaagca taatccatca taaaatcagt
     3241 ataggtgaaa agatgaactt tcatctgcat atcaagtaat tggtatgagg cattttttga
     3301 ggtgtaggat atgaagttgg attaccttat agaagaggaa gatagagaaa aactgctgct
     3361 agctagatat ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt tatatgaaac
     3421 attaggtttg tctaaattta aaacaagaaa ttatatcata gaattaaata aagaatttac
     3481 tgaattcaaa acaaagccag agatccaaat ttcagaagaa gcagagatcc atgtcgaacg
     3541 tctgaatttg acggatatca aaaatttaag aagaatctac tttcaacgat caaaaattgc
     3601 tcgactgtta acggagatcg tggattcaaa agtaacgatc gaaaaatttg cagagaatca
     3661 ttttttaagc atatcaagag cctatgtaaa aagaagagaa ttagtctctt ttcttgaaca
     3721 gcatcaaata aagttaaaga aaaacatatt agttggggaa gaaataaaca tacggaacgt
     3781 attatttggg gtatatttcg gggtctttaa tggtttttct atgccattta gagaaagcgt
     3841 catgaatgaa gcaaagagta tcgtgcaata ttttgaatat ctgttcaatt taaaactgtc
     3901 tgcaacaaaa cgaatcaaat tcaatttgct aaccactatt agtttgttga gggcctatgg
     3961 gaatcaacca atcggaaaac gtttcttttc agatgaatac tttaagagct cagaaggaat
     4021 gaagatcatc aaggatttat cacatatttc ttttcttcat atccctaaag agcagatcat
     4081 tgatgagatt tcttatatca tgttgttttt gatcacggaa gaagaatttg aattagatgg
     4141 cgcatttgga cttgcggaag ctcattttca tgatatccac atgattagtc aaaaaatgtc
     4201 agtggaattg attgatcaat taccattttc gattgaaatc tctgaagaag aaagactata
     4261 tatacgaaaa gaacttcgta tgggtatgat gcatgtcaat cgaaaaaatt ggatattcga
     4321 ctttgagatg actagcttta cgtctaaaaa gcagctacag tttttcttcg agacgtatcc
     4381 aatattcagt acagcaatcc aaaaggtcat acaagagaat aaacgctatt tcttgggaaa
     4441 agatgaacgt ttcatgattc gcttatttta tgattatctc tttttattgg tcagtatttg
     4501 tccggtatca tattttgaag caccgattca tgtctgtatt gatttttcgc aaggatctgc
     4561 ttataccgat tacattattt ctcaagtgaa aggatttaaa aattacaata ttgtgattga
     4621 gagtcgaatc acttctcgta cgaatattta tttatctgat tgtttacttg aaaaatttaa
     4681 taaagaacag ataatttgga
//
LOCUS       DAZBSA010000145         4654 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000145 DAZBSA010000000
VERSION     DAZBSA010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.148"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..943
                     /locus_tag="ACHGMT_002655"
     CDS             <1..943
                     /locus_tag="ACHGMT_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFN6836351.1"
                     /translation="HDFYQKERYWFSFPKNVHLFTSETVETKKITIKSGETVSLSGFS
                     YRQPWIQKDKVMEFPSRELTDYHIGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALG
                     HIHVPTVLNEKQTIVYPGAPQGHTKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTK
                     EISLRKARTTKEVIVHIRQQLSKVEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYL
                     LEEFSDRINDFFIWRISLIENTFYTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYS
                     HQEAARILSELPEYQEETLEKAKQLLNQDFLFEEDQE"
     gene            940..3660
                     /locus_tag="ACHGMT_002656"
     CDS             940..3660
                     /locus_tag="ACHGMT_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN6836352.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            3644..4588
                     /locus_tag="ACHGMT_002657"
     CDS             3644..4588
                     /locus_tag="ACHGMT_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFN6836353.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         1718 a          776 c          946 g         1214 t
ORIGIN      
        1 tcatgatttc tatcaaaaag agcggtattg gttctccttt cctaaaaatg tccatttatt
       61 tacatccgaa acggtagaga caaaaaaaat aacgataaaa agtggagaaa ctgtctctct
      121 cagtggattt agttatcgtc aaccgtggat tcaaaaagac aaagtgatgg aatttccttc
      181 tagagaactc acagattacc atattgggtt gtaccacggg gaacctggcg tctctcaaaa
      241 aggaaactat gctcctttcc aaccctcaaa gatgcaagaa aagggatacg attattgggc
      301 tttagggcat atccatgtgc caacggtctt aaatgaaaag caaacaattg tttatccagg
      361 tgcgcctcaa ggtcatacta aaaaggaaca agcttctaca tctattctac tagttgaatt
      421 aagtaaagga agttgccaaa tacatccgat taaagtagca gaggtctatt ggaagacaaa
      481 ggaaatatca ttgaggaaag caagaacgac aaaagaggtc attgttcaca ttcgtcagca
      541 gctttcaaaa gtagaggatg gatttagttt aattgagata aaattaaaag attatgacca
      601 tctcaataca gatgttttgg aacggataca atcaggagag ctattagact atttgttaga
      661 ggaattctca gataggataa atgatttctt tatttggcgt atttctctca tagagaatac
      721 tttttatacg aagaccccac ttgctgcttc tgctaaattg atggaacagt tatttcaata
      781 ctatcagaca cctcaagatt tccaacaaat tttaaatgaa gtatatagtc atcaagaagc
      841 ggctagaata ttgagcgaac tgccggaata tcaagaagaa acacttgaaa aagccaagca
      901 gctgcttaat caagattttt tatttgagga ggatcaggaa tgaagatttt aaggatcgag
      961 attgctgctt ttggaaaatg gcgtcaaaaa agttttgact tttattcagg caatcagctg
     1021 atctatgggg gaaacgaagc aggaaaatca acgatttatc aatttattca agcaatatta
     1081 tttggtttcc cttcaaaagg ccgtaaaaaa aaggattata caccaaaaga cggctcagcc
     1141 tatggcggca aaatatggct taaacatcct gtatatggtg aatttgctgt cgagcgctac
     1201 aagcagcaaa atcgcggaaa atcgaaggtg tggctggggg atcaagtggg aagtgatgag
     1261 ttactagaaa aactgatcgc acctttgacg aaagagttat tccagcaggt atttactttc
     1321 cagcaagagc agttgatgga actagatcat ttgagagaaa aagagctaca tgatgcgctg
     1381 atttctttag gcatcacggg cagttctcaa gtattccaaa agcggcaaga atattatcag
     1441 aaagcacaac aattattcaa gaaaaaaggc caaaaattac taatcaatca aaaattaaat
     1501 gaatggaaag agctgcaaga aaaaattcat caaaaacaag aacaggaagc ttattttggc
     1561 aagttgatcc aacaagaaca ggaatacgag tcgcaacagc agcagttatt ggcacaatta
     1621 aaagaggcga acaaacaatt ttcattagcc agacagcaag aacttaattt ttctttatat
     1681 gaagaatggc aatctctcaa aaaaaacagc actacaggtc ttcctgatga gcaacttctt
     1741 ttagatctgg agtctttctc caaacggtat cagcaaacaa atgagcgttt ggaagaatta
     1801 gaagcaacac tggaaaagca tagcgggatg gatcagcagt cagccagata ttatttttat
     1861 ttagagcagg agcaggaact aaaagatctc cagcaacttc aatttacagc ggaacaatta
     1921 aacgaagaat ggcaaagatt acaaattgag actagagaaa caaattcaga attgaagatt
     1981 ttggaacaaa ggtggcattg gcagaaaaat ccgttaccgg aagaattttt atatgaagaa
     2041 gaatggaagc atcttctggc tgaagaaaga aggattgctg atctagaaag ccagatttct
     2101 gtgcgtctcc aaatagctaa agaacagcaa gagatgattg aagcagaagt gaatgacttg
     2161 gaagagacct atccagcatt attacaaaat cagtcagaga tagcaccagt ctcttcatct
     2221 aaaccgtata tgctgatggc tggcggagca ataatcattg caggtatatt tgtaccaatg
     2281 cccttgaaag tgatcctttt actaattggt gcaggcatga ttggctattt cttctacaaa
     2341 aggcaaccaa aaattaaaac taaaaatgat ttctctgaga taaaaggaat gtggcaagaa
     2401 aaactaggac aacttgatct agccttagct caattagctg aagcagaaga aaaacaaaag
     2461 agaactggag aagaaaaaca gattcttcag caagaagtat atcgttttgc ggatcatcgg
     2521 cacttaggaa agatggatac gctcaaactc atgaaagaat atggacagga agtcatagat
     2581 tatcagaaat tatatggcgt atttgagcgg aaacataaaa gagaacagga aatacaagaa
     2641 aaactacgac aaatcgacca aaaatttgag tttgttaaag aatggttacc gatacaagat
     2701 aagacaattt cagaaaaaat ggtattgctt tcccgtttta tacaagagat ggaacagatg
     2761 aaatttgcca gaagctatca gcaaaatact ttattgaagc aagaaatcaa tcaattgaaa
     2821 aagaaacaaa aagtatggat acaagaatac agcgaacaat tgactcaagc aggcttatct
     2881 tatccttcgg aagtacctgt ctttttacaa aaagccaaac aagccgaaca gcagatgaaa
     2941 agatacaatg aattatcgga aatgcttact ccactatttc ctgaagagct gacaaaagag
     3001 aaattatctg taagactgcg ccaactgaaa gaaaagcaat cccaactcca gcaagaagaa
     3061 aaagaagcaa gtgaaaatcg gcaacgactc caacttcaag tagaagagtt gcaaagagac
     3121 ggcacattgg acgaacttta tcaacaagct agccggcaaa aagcaaaatt acaggaatta
     3181 ctggaggaat ggggagcact tgaaatcgcc gggacattct taggtgatct ttcaacagag
     3241 atgagtgaaa gacaattacc ccagttgtta agagcagcag gtgattattt aggtatttta
     3301 acaaaaggaa attatcagaa gatattactg aaaaatgata cactgacttt aacagacgga
     3361 tatacagaat ttcccatcta tgagttatct acaggtacga aagatcagct gattatggcg
     3421 atgagatttg cttacctctc attagaagct aaccgttcga tatgcccgat catcattgat
     3481 gatggctggc ttcattatga tcatcagcga aagtatcaat tagctgaact tttagctaaa
     3541 tttggagaaa agtatcaaat aatttgtttt tcttctgata aagaaatggt aagctattac
     3601 catgagatga accaaatcgt aaaacaatta gaaggagcaa ataatgaaaa aaattcgtga
     3661 tttagtaatc gatgagcagt ttgaacaatt tgtcctgatc aaaagtgcag atgtaagaat
     3721 cgctaaaaac ggaaaaaaat ttattgcttt tactttccaa gatacttcag gtactatcga
     3781 tggtaaatac tgggatgctt cggaggagga aatcaataaa tttacagccg gcacagtcgt
     3841 ttttctaaac ggtaaaagag aggtttatca aggaaaccct caagtaaaga ttcttcatat
     3901 gcgcttaaca agggtagatg agccaggaga gcctagttta tacatggaaa gagccccttt
     3961 gaagaaagaa gaaatggaag aagaaatcaa tcaattgttg tttgagatca ccaatgcgca
     4021 ttggaatcgt attgttcgct atctacttaa taaatatcaa aaacaatttt ttgagtttcc
     4081 ggcagcaaaa agaaaccatc atgcatttgc cagtggatta gcttatcata ctttgacaat
     4141 gcttcactta gggaaatcaa tcgccgatga atatcctgag ctgaatcgag cattgttgta
     4201 tgctggtatc attttgcatg atttaggaaa ggtgattgaa ctttccggtc ctatgtcgac
     4261 agaatatacg cttgcaggaa atttgatcgg tcatctggta ttgattgacg aagaaataac
     4321 taaagcctgt cttgcgttga aaatcagcga agctgacgaa gatgttctcg tattacgtca
     4381 tatggtttta gcacaccatg gcttattaga atatggctca cctgtacgtc caagaataat
     4441 ggaagcagaa atattacatc aaatcgacaa tatcgatgct tcgatgcaga tgatgttgac
     4501 ttcgattcgt caaacagaac caggaaaata caccgatcgg attttcggta tggacaaccg
     4561 aagtttctat gtaccaaaag atttatagca aaaatttatt tttttcattc aaaacaaaaa
     4621 aagcgagaca taagtcttcg ctttttttgt tttc
//
LOCUS       DAZBSA010000146         4591 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000146 DAZBSA010000000
VERSION     DAZBSA010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.149"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            186..2855
                     /locus_tag="ACHGMT_002658"
     CDS             186..2855
                     /locus_tag="ACHGMT_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFN6836354.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2946..3560)
                     /locus_tag="ACHGMT_002659"
     CDS             complement(2946..3560)
                     /locus_tag="ACHGMT_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN6836355.1"
                     /translation="MINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKAMH
                     MVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPGIE
                     KRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTIIK
                     ITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3680..4219
                     /locus_tag="ACHGMT_002660"
     CDS             3680..4219
                     /locus_tag="ACHGMT_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836356.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGEMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4216..4554
                     /locus_tag="ACHGMT_002661"
     CDS             4216..4554
                     /locus_tag="ACHGMT_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HFN6836357.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1421 a          790 c          859 g         1521 t
ORIGIN      
        1 atttttaagg gtgggacttt tgattcctac cctttttttg cgtttatttt tgttatacga
       61 acaaattatg aagttattta ttctaaattt tatttttttg tgaaaattag actcttttta
      121 agcagagaaa aacaaggata tgataaactg aatacaactt catagaaatt tggtggaaaa
      181 taaatatgaa aaaaaattta aagtcattct ttagagaaaa cggacgctac gcagcagcta
      241 gtttcctgat tccgtttttg attttagcac ttgtttatct aagtatcggg atctatcctg
      301 gaagcagcag aagtattttg gcaagtgatg ccttttcaca gttttctaat tttcatgcca
      361 gcttccgaaa tgttcttctt ggaaagcaaa gtatttttta tacatggaat gcttctctag
      421 gattgaatta tttatcttta atttcttact atctaggcgg gatcttcacg ccactggtgt
      481 tcttttttcc aaaccagatg atgccagatg cactgtatgt tctgactttg cttaaggtcg
      541 gctgtgccgg attggctttt tggtttcttg cacgtacgtt caagattcct cgctgggggc
      601 atgttgcact tagcgtcagt tacgcattga tctcatttat cactgcacat tcggagatca
      661 tcatgtggct ggatgcattt gtttatcttc cattagtgat tttggggatt catcgtgtta
      721 tggatcttaa acggccgaaa cttttattta tcagttattt actcttgttt ttatccagtt
      781 tttatatggg ttttatgatt ggcgtatttt ctttcttata ttttatcgcc cgtctattga
      841 caaactggtc agaatataaa aaaacgattg ttccttatgg tatcacttct ttactggcag
      901 gcggcgcttc aatgatcatt gtattgccag cagtgctgga tcttcggtca aatggcgagg
      961 cactgagtca gatcactacg ttgaaaacag aagcgacttc ttatctggat ctaatcatga
     1021 aaaatatgat tggtgtttat gacacgacaa aatacggatc gattccgttc gtttacgcag
     1081 gtctgctacc attagtcctc tgtctgtttt attttgccag tcggaaagta ccgttgaaaa
     1141 ataaattttt gtacggcagt cttttcgcca tacttatcgc aagtttctat cttgttccat
     1201 tgaatttgtt ctggcatggt atgcatgcac cgaatatgtt tttattccgt tatgcctatc
     1261 tgttctcttt cttagtgctc ctgcttgcag gaaaaggatg ggaaaagata gaacaagacg
     1321 atcgtgggct gttgttagga atcattttgg tcgtagctgc tctctttgcg gtggcatggg
     1381 gcatcaaacc tgaaggaagc tacacctatg tgacgatgac ttcctttgta ttgacagtgg
     1441 ttttcttagg gatttatgct tttgctgtgg gattttatca gcataaaaaa ctttcattca
     1501 atcatctgag tatattgtta ttattgatca tgtctgctga agcactggtc aatacgaaca
     1561 gtatgatcca tggcattctt gatgattgga attatgcttc ccgcagttta tatactgatc
     1621 cttatcctga cttgaaagct cttgtaaatg aaacaaaaaa agattccgac tcgttttacc
     1681 gcttggaaaa tctggatcca gtatctgcaa acgattcgat caactatggt tacagtggta
     1741 tcagtttgtt ttcttctata agaaatcgta attcttcctc ttacctggat aaattaggtt
     1801 tccgttcaag agggacaaat ttgaatatcc gttataacaa taatactttg ctaatggatg
     1861 ggtttacagg aatcaaatat aacatttcta aaaacgaaag cacgttcagt aaatatggct
     1921 tccgccaagc tggtcaaagt ggcgactata cactgtatca gaatgtcaat gcattgccac
     1981 tagcatttac tgcaccgttg tccgttaatg atgttgagca gccagtgaca gataatttga
     2041 ccagtcagac aaacttgatc aatacattgt ctggtttaaa tgaacagtat tttacattct
     2101 atacacctac tttgaagaat cagttgaata cgacgatcac aaagacaacg actggcgtca
     2161 cttactcaga aacagcagat aaccagccaa aagagctcac ttgggaagtc catgtgcctg
     2221 caaatacaca agcttactta agtttgttcc caaccaattt tgcacaatta gaaagttcta
     2281 gtgcgacgat cactgtcaat ggtacatcgc gaaaaacgca aatcaacatt tctggtcaat
     2341 actatgatct tggctattat cctcaagaga caacagtgac atttactgca agtttttatg
     2401 gaacgaaaga agtaagtttc atggagccta aagtcgttgg tctagatgtt gtagcctatc
     2461 aagccgcaat gaatcagatc aaagaaaaag gtgtcgaaat ggaagccact ggcagaaaag
     2521 cttctgggac tgtaaatacc gctgaagatt ccatgttggt gacaactatt ccttatgaca
     2581 aaggatggaa agctaaaatt gatgggaaac cagtcaaggt cagcaatttc aaagacgcat
     2641 tcttgatgat aaaagttcca gcaggtaaac atactgtcac tttcagttac ttgcctgaag
     2701 gatttacgat aggtgcaatc ctttttgtcc tatgtattgc attgtttatt ctttatgtct
     2761 ggtaccttga ccgcaatcgc cccttttatg gcacgattga tccagcaata gaagaaaaac
     2821 cgcgaaatac acacagaaga agacgaagaa aataaagaaa cgatggaatc tgtgtcactc
     2881 ttctatttaa atggcaaaaa tttcaatctt taaatgacaa aaaccacctg atttttggtg
     2941 gttttttata tacctatgac taaactatct cttgctgctt cgacaacttc tacagtgatc
     3001 ttatccattt gattgattct taaaatccga tcaatctgcg caaataaacg atgaataagc
     3061 ctaaaatttc cttttgtgat cttaatgata gttgtgatag cctcataatc agaaaaatct
     3121 tctagcttga tatcaattcc taaatctgcc catttgtatt ccaaaatatg atgtgtttcg
     3181 tccttactaa ggttatcaaa ttcatgtgca aatcctatgc gagagtaaag ctgcggataa
     3241 cgagacaatc ttttttcaat accaggcata ccgataagaa ccattgccag atcatttcta
     3301 tcgtaaatat ctcttagctg ttctaaatgc tgaagcttta aacgatcaat ttcatcgaca
     3361 ataattaagt caattgcttc ataggtttta ggcacaggag gatcttcttg attgtatttt
     3421 agtaccatat gcattgcttt agtcagacct attctactag ttattgtgtt aatatccgaa
     3481 ctgacacgag acatcttttc agctggtaca gtataaaaaa ctttatttac acttaatatt
     3541 tcatctgttg catttatcaa gaacaagaaa aagtctgaat attcaaaaaa aataacggtg
     3601 tacattttta aaaggagatg tataatcgag atttagaaag cgttttcctc aaaatttaat
     3661 gaattaggaa gtgattacta tgaaaaaatt atttagcgca ctttttattc tgttatttgg
     3721 ctttattggt attgggactt ctggaaatat agttaaagct agcacgatgg ttgaacaaac
     3781 aagtgaaatg gcaaaaactt ttagtcaatt aacaccagaa gaacaaagtt acttcaaatc
     3841 caaaggattt agtgataaag atacttttta cactcaaaag atagagcctc aagatccgaa
     3901 aacacgtttt tctgcaaatg tattgcggtt aaccgcatct actaaaaaaa ttactaatac
     3961 gcaggggcgg acagaatata ttataacaag tcccaattcg gcgatgtatc gtatttctac
     4021 acgcttgagt ttaggtagtg caaaatcata tactcaaaca gtaaatctag ctaattctta
     4081 tggttatggt ggagaaatgt attttacatt tactgggaaa agacaatatt acagtgtaaa
     4141 attagctatg caagtttata atacatttgg tgtagggtca gtctcttgca ctgcgggagg
     4201 attgacaata ggaaaatgat aatttcttac tatgtcttaa atagaagtga tgatgaagga
     4261 ctcttcgttt ttcaatcatt acatgaggat acgatgctaa aagagtttta taatcggatt
     4321 aagaataaac agatagaaac aaatttatat tttttaagtg actctatctt gccttttatt
     4381 agcaacccta aaattcttaa cttgttgcag acaaaagatt tgaaaagttt accgattatt
     4441 gtcattgatg aggaaattgt aaaatatggc gcttatctaa gcgtagaaga attagaaaaa
     4501 ttaactaaca ttggaatgag tattcaggag gataatgggg tacatggaac atagaatgaa
     4561 tatgcacagt tgagaacgga atttgacatt t
//
LOCUS       DAZBSA010000147         4556 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000147 DAZBSA010000000
VERSION     DAZBSA010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.150"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      227..393
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            541..1098
                     /locus_tag="ACHGMT_002662"
                     /pseudo
     CDS             541..1098
                     /locus_tag="ACHGMT_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1156..3195
                     /locus_tag="ACHGMT_002663"
     CDS             1156..3195
                     /locus_tag="ACHGMT_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFN6836358.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDQYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGEKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKDDDDSATKRASELLQYLNQNYQ"
     gene            complement(3304..4455)
                     /locus_tag="ACHGMT_002664"
     CDS             complement(3304..4455)
                     /locus_tag="ACHGMT_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFN6836359.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVFLLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
BASE COUNT         1667 a          833 c          875 g         1181 t
ORIGIN      
        1 aaatatttat tccgaaaagt gaaaaaacat ataaaaaaac acataaaact aaaaaattat
       61 caaagaatcc tggtgcaact gaaagaattt gcaacgggat tctttcaact tatttgttat
      121 ttttttctaa aaaatattaa agattgttaa atttcactat ttcatttcaa attccattgc
      181 ctaatcatta ggaaaagtct atcatgaact cgtaaataga atatagtaat aaccttgtta
      241 ggtgaggctc ctatacaaac ataggctgtt gcccagaaac gtcgaaagtc gccaatgggt
      301 aaaacagaaa ctgttggatt aaggcttttt ccaatagcta agaatgtttt ctttacgttg
      361 tatagtgcta aaactcggac gatgagatga tgctaggtca gaattcttgt tcgaaaaggt
      421 ttctgacctt ttctttatat ttttgtgact gacttatttg atgagacaaa ctcgctcctc
      481 acctgacaag tggattgctg tgtgaggagg agatgcggtg aaaaaagatc gattggtgga
      541 ggtcgctcgg attcacttct ggttgcacaa tacaaccaga aaacacaaca agcaaaactg
      601 atctctatca tgagagattc atatgtcgat attccaggtt acggaatgaa taagataaat
      661 gcagcttact cttatggggg agttgattta ttaaatcaaa cattaaagga aaatttcaaa
      721 tttgaagctc cctattatgc aagtatcaca tttcaagatt ttatcgattg cgttaatgaa
      781 ctgtttcctg gtggagtcaa gatcgatgca gaaaaatctc tagatttaga tggcgtatat
      841 ataaacaaag gagagcaagt gatggatggt aatactttac tccagtatgc tcgatttcgg
      901 gaagacaaag aaggagactt cgggaggatc agaaggcagc agcaagtgat caaagctatc
      961 tctcagcagc tgaaagacgt aacttcaata ttcaagctac ccaaagcggt tgggaaatta
     1021 cttggtagta tccaaacgaa ccttccagaa agtgtgcttc tggattgtgg tatggatttc
     1081 ctcaaagaca tatgttagaa taaacaggta taaatagtga aataaaggaa tgacaagcaa
     1141 gagaaggagg aaaaaatgaa aagaagtgac aagcacggca aaaatcgaac aggcgcttat
     1201 attgccggcg cagtgatctt aatagcagct gcgggtggcg gttattttta ctaccagcac
     1261 tatcaagaaa cccaagcagt agaagctgga gaaaagacag ttgagcaatt tgtccaagct
     1321 ttgaacaagg gagattataa caaagctgca gaaatgacat caaaaaaggc ggcaaataaa
     1381 agtgcattat ctgaaaaaga gatcttagat caataccaga atatatacgg tgctgctgat
     1441 gtcaaaggac ttcagatatc gaatctaaaa gtagataaaa aagacgattc tacttacagt
     1501 ttttcatata aagcaaagat gaatacctca ttaggtgaat tgaaagatct ttcttataaa
     1561 ggaacattag acagaaatga tggtcaaacc acgatcaact ggcaacccaa cttggttttt
     1621 ccagaaatgg aaggaaatga caaagtaagc ctgaccacac aagaagcagc aagagggaat
     1681 attatagatc gaaatggtga accattagca acaacaggca aactaaaaca attaggagtc
     1741 gttccaagta aacttggaga tggaggcgaa aaaacagcca atatcaaagc gattgcttcc
     1801 tccttcgact taacagaaga tgctatcaat caggcgattt cacaaagctg ggtacaaccc
     1861 gattactttg tcccattgaa aatcattgat ggagcaacgc cagaacttcc agctggagct
     1921 accattcaag aagtagacgg cagatattat cctttgggtg aagcagctgc tcagctgatt
     1981 ggttacgtgg gagatatcac agcagaagat attgataaga atccagaatt aagcagtaat
     2041 ggaaaaatcg gtcgctcagg tttggagatg gcttttgata aggatcttcg tgggacaaca
     2101 ggtgagaaat taagcattac agatgcagac ggtgtagaga aaaaagttct gatcgaacat
     2161 gaagttcaaa atggcaaaga tatcaaattg acaatcgatg cgaaggcaca aaaaacagct
     2221 tttgacagtc taggaggaaa agctggatca actgttgcga caacgccaaa aaccggtgat
     2281 cttcttgcgc ttgctagctc tccaagctat gatccaaaca aaatgacaaa cgggatctca
     2341 caagaagatt acaaagctta tgaagaaaat cctgaacatc cattcatcag ccgatttgcg
     2401 acaggttatg ctcctggatc tacgtttaaa ataattacag cagcaatcgg tctcgacaac
     2461 ggcactatcg atccaaatga agtgttgacg atcaacgggc ttaaatggca aaaagacagt
     2521 tcttggggat cttttaaaac aacgcgtgtc agtagtgatg tatcacaagt agacttaaaa
     2581 actgctttga tctattccga taatatatat gcggcacaag aaacgttgaa aatgggtgag
     2641 aaaaaatttc gtacaggctt agataaattc atttttggtg aagaccttga tttgccaatt
     2701 agtatgaatc cagcacaaat ttctaatgaa gatagcttta actcagatat cttgctagct
     2761 gatactggat atggacaggg cgaacttcta attactccta tccagcaagc agcaatgtat
     2821 tctgttttta ccaacaatgg cacacttgtc tatcctaaat tgattgcaga taaagagaca
     2881 aaagataaga agaatgtcat cagcgaaaca gcagtacaaa cgatcgtgcc agatctgaga
     2941 gaagttgtgc aagatgtaaa tggtacagca cattctcttt ctgctttagg gattccgttg
     3001 gcagcgaaaa ctggtacagc ggaaatcaaa gaaaaacagg atgtaaaggg gcaagagaac
     3061 agtttcttgt ttgctttcaa tcttgataac caaggttata tgatggtcag catgttggaa
     3121 aataaagatg atgatgattc agcaaccaaa cgagcatcgg aactattgca atacctcaac
     3181 caaaattatc aataaaaaat tgctttttaa gtacagtaaa aggactgtgg cgttatcgtc
     3241 acagtccttt tttcaaataa acggttttca cgatgtctct ttcacaactt ctttttgatt
     3301 tactcacact tcataaactt tttttgtaaa atatttgaga acaagtggag aaataagtgt
     3361 acttaataaa atcacgataa ctaaaggtga atagtactgt ggttcgatca attgactttg
     3421 ctgtccaatt tgtaaaatga tcaaagccat ttctccacgc gaaatcatac cagcaccgac
     3481 catcaatgaa ctatgttgag aaaaaccggc aatctgagca ccaaaataac ctccaagcaa
     3541 tttggtcaaa atcgctacaa gcgtcaatat agaaataaag agtagttgtt cactaaagtc
     3601 agaaaaatca acttctaatc ctacactgac aaaaaatact ggtataaata ctgcatatcc
     3661 taaagcctct acattgtggt atacttcttc tttaaccttt gtctgactga cagctatccc
     3721 agcaaaaaaa gcaccaatga cagagctaag cccaacaaga tccgctacgt aagacattcc
     3781 taaacaaata actagcgata cgatgataat agaagaattt gcgtacactt tttctgcaaa
     3841 tgaaagtagg aaaggcgcaa tccatttgac taataagaaa ataaaaacaa aataaattag
     3901 ctgttcaatc aaaacaagcg gtaaagaagc attgctagaa gcttcccccg ttagaaaaga
     3961 taggctgaaa cttagaacta aaacgactaa aatgtcatct accacagaag cacccaaaat
     4021 tgttgatcct tcttttgtat tcactacgtt tagttctttt aatacttcta ctgaaatact
     4081 gacagaagtt gccgcaagaa taatcccaaa gaaaatcgct tcatttcccg aaacttggaa
     4141 tgctttgcct gtcaaccagc caaaaacaac cgggaagatg atcccaagca aagcaacgaa
     4201 cataccaggt cggaaatatt ttttcaaaag agataaatca ctttccagac cagctaaaaa
     4261 cattaataag aacacaccaa tctccgaaaa gtcatggact agaatatcag gtttgaccca
     4321 attcaatcca gcttggccta gcaaaacacc aacaagtaat tgtccaatca ctgctgggat
     4381 acctaatctc cgcgagatat gtgagccaat cgttgttgaa atcaatatca ggcataaaat
     4441 accaataaat tccattctca tcattccttt gtataaaata tagcccgaac ttagcataac
     4501 atacattatt gaaaaaatag gaaaatatac tcacaaaaaa accaacaatc caaaag
//
LOCUS       DAZBSA010000148         4401 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000148 DAZBSA010000000
VERSION     DAZBSA010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.152"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            74..955
                     /locus_tag="ACHGMT_002665"
     CDS             74..955
                     /locus_tag="ACHGMT_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFN6836360.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            968..2578
                     /locus_tag="ACHGMT_002666"
     CDS             968..2578
                     /locus_tag="ACHGMT_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6836361.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2655..3314)
                     /locus_tag="ACHGMT_002667"
     CDS             complement(2655..3314)
                     /locus_tag="ACHGMT_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFN6836362.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVALYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3597..4262)
                     /locus_tag="ACHGMT_002668"
     CDS             complement(3597..4262)
                     /locus_tag="ACHGMT_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFN6836363.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
BASE COUNT         1180 a          857 c          871 g         1493 t
ORIGIN      
        1 aggtataaaa cctttttatt aaactattta atcttgttta gtatatattt ttagaaaaag
       61 gagtggggtt tttatggcag aaatcgtcaa agtcacacat ttgaaaaaga gctttggtcg
      121 tttcgaagct ttaaaagatg tctctttcac cgtacattca ggtgaagtcg ttggatttat
      181 cggaccaaat ggttctggaa aatccacgac gattcggaca cttttaggga ttataaaaaa
      241 agacggcgga acggcaacga tttttgggca agatgtatgg gaagatagtt tatctatcca
      301 tgaacgtctt tcttatgttc caggcgatat ctctttgtgg ggaaatttga ctggaggagc
      361 gatcatcgat ctcttcatga agcttcatgg aaaagggagt gctcaaaaaa aagatgaact
      421 gattcaacgt ttctctcttg atcctaagaa aaaagcaaaa aactattcaa aaggaaatcg
      481 ccaaaaagtg ggcttgattg ctgctttggc tgttgattcg gatctctatt tatttgatga
      541 gcctacttct ggtttagatc ctttgatgga aacgattttc caagaagaag ttgaaaaatt
      601 gaaagaacag ggcaaatcca tcctactttc ttctcatatt ctcagtgaag tcgagcgact
      661 agcggataaa gtcgtgatca tacgcgaagg aacactcgat gaagaaggaa cactcgatga
      721 attgcgtcat ttgactcgtt cgaccatcac tatagaaaca gaagcagaga atagtcactt
      781 agcacaattg aacggtgtcc atgactttgt gcaaaaaggt cacctcgctc aatttgctgc
      841 tgataacgaa gctttcgatg tgattttagc tgaagcgtcc aaattaggtg tgaaaaaatt
      901 tgaatctatt ccaccgactc ttgaggattt attcatgaga cattatgaag gatgaggagg
      961 aagcgaaatg actgataaat tttcacgctg gtccaccctt ttttctcaaa accttagccg
     1021 tgactggaaa aagatcctga tatggctagt cggtgtcact gctttttctg gtggttttgt
     1081 ccctgcgttt gaagaattga cttccggaca aggattagct ggattatacg aaaccttgca
     1141 aaacccggca atgattgcaa tggtcggacc aactccagct tcgaatgctg ctgagtatac
     1201 tttaggagcg atgtatgcaa atgaaatgtt gcttttttgc agtttgtttg ctgctgtgat
     1261 ctctgtttta cacgtggtga gccaaacgag aaaaaaagaa gaacttggtc tgattgaact
     1321 tattcgttcc tttcgagtcg gacgtcaagc aaactcttta gctgtcatcg ctgaaagtat
     1381 tctgatcaat ttgcttttag caatatcgat cagctgtacg atgatttttt tccaagcgga
     1441 ttcgatcact gcagaaggtg cttggctttt tgggctatct gtaggtagta tggggatttt
     1501 ggggagtgcc gttgcactgt ttgctgcaca actgatgcca agttcagcca gttcgaatgg
     1561 tctgtcttta gcgtttttag gtggacttta ccttctacga gcaataacag atgtagctga
     1621 ttcctccctt tcgatgtgga atcctttagg ctggtcttat ttgacctatc catttacaac
     1681 taacaactgg attcctttgc tttatttgtt tttgttcagt ctaatcttga taatcagttc
     1741 atttgtttta gaggaacatc gagatttgaa caccggctat atccccgaac gtgaaggaaa
     1801 accgtatgct aaacgttctc ttttatcctt gcgtggttta ttattctatc taaataaagg
     1861 gatgacaatc agttggctta tcacttttgg tttgatgggg gcggcatacg gctctatcta
     1921 cgggaatatg caacagtttt tagaaagtaa cgacttaatg aaacagatgt ttatccaatc
     1981 tggcgtctca attgaaaaaa actttacaag cacgatcatg gtagtcttga tttgtctagc
     2041 tgctatttta ccaattgcta gcatcaatcg tttatttaca gaagaaaccc acggacgtat
     2101 gaatcagctg ttcgcaacaa aaatttctag gaaccaactt tattggacca atatgttact
     2161 agctattcta tttgctatat tggcaatcct gattagttct ggtgcactag ggctttctgc
     2221 tttggcttcg atgaaggatg cgaaagacat ggatctattg atgtttctta aagccgggtt
     2281 caatttcctg cctgccgttt tagtgtttat cggaattagt ggctttctat tcggatggct
     2341 tcctcgaatc ggtaaacttg cttatatcta cttagcttac tcctttgcac tcaattactt
     2401 tggcggaatt ttggatttac cagaatggtt ttccaaaaca gctgtcccta gctgggttcc
     2461 acggatgcca aacgattctt ttgatctttc cgtgttcctt acgttatcat gtatcagcct
     2521 tgcactgttc gtccttggtt ctattggtta taggcgaaga gacttactag aaggataata
     2581 aaaaagcatg tacaatttca tgctagcctc caaatctggg tcaaaaatcc aacatttgga
     2641 ggtcattttt ttatttacaa gtcttctaac tgctctttat caatgatcca gacttctcga
     2701 ttctttcttt ttacataacc attctcctct aataatttaa attttctaga taaagtttcg
     2761 ggggtcgtac cgataaatgc agcaagttct ttcattttca ttggcaggaa aaagtgatcc
     2821 ttccctgtcg ctttagctaa gtcaagcaga tagagagcga ctcgttcttc tactttttcc
     2881 atcatcaaaa aagttgtctg ctgctgtact tttgttaatt tctcaacgtt gattgctaaa
     2941 agtttcagac taagggcggg ataatgcagt aagagttccg agaaatcttt ttgtctcaat
     3001 atgcaaattt ccgttttttc tagtgcttcg ccaaataaag actcatttgg aacgcctagc
     3061 aactggctct ctccctcata tccgcccggc tctattactc gtaaaagctg ttcctttccg
     3121 ctagcagata attgatacac tttcatactg ccggaggcca cgatcactaa ttgaggttct
     3181 ccattcggcg caagaatctg ttctcctttt tccacatagt gatgtttgac aagtgcgtgt
     3241 actttttctt gatcttgctg tactaagtga ttgaacaaag gaacaaatga aacacataaa
     3301 tgatcatgac tcataatctc tcctccttct aaacagctat ttctaataaa gaaacataac
     3361 ttcccttatt ccaactgata attttctttt atcatagcat aaacgtcaag cgaaccagat
     3421 accagcaaat agagtggatt tctttttgtc ccttgcttta ttttccttct tttgagacct
     3481 taagtaattg attcctatca attttttaaa tatttacttt ttgaataatc aaaaacaaac
     3541 cttcactcgc aagccgagag taaggtttgt ttttcgcatt gcttatttcg tgttgtctat
     3601 tgaaattttt ccgcacctag cttttgatat agtggtgcta gttcttgtaa ggtttcagcg
     3661 gttatttgct caacgtgttc gatttcttgc ggatagaaga atagtttacc catttgaaag
     3721 gttcggtctc tcatcgcttt tttcaactct tcttgtttct ttgtatcgga caaatagtca
     3781 gacaaagcta agtggtcgtc aagttcatgt tctggttgcg gccatatgta ataatccttg
     3841 atatcgactg ttttgctcca ctctttcagt atcgcaagca actggttata cgctgcaatg
     3901 ctaccatatc gttcttcgct tttataatgt tggtacatca aatagatttg gtattgtttt
     3961 tgattcaaca aaactgctag acaggcattc tcgtcttgac gagattcgct tcggtacgcg
     4021 ctccaaaaat gactgcgtaa attccagcca ttcgtccagc tttcgatttt aggtttgctc
     4081 atgccgtaca tatcaggcaa aagtgcagct gtctggagct gaatctcttt ccatttttgc
     4141 cagtgttctt tatattcttt cttgatttgt tcaatcttat ctgttggatg gctctctttg
     4201 agctgtttga atgcgtagat tggtcgatca aacaactgat aatctgattt tccaagttcc
     4261 attgtttcgc ctctttccgt tatcttttgt attctacctg aattttgcga tatggacaaa
     4321 tagaggttgt gaaaaagctg ttctgcatca agtcataaga acagccaaac aaaaaactgc
     4381 gatgagcatc accgcagttt t
//
LOCUS       DAZBSA010000149         4394 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000149 DAZBSA010000000
VERSION     DAZBSA010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.153"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            195..896
                     /locus_tag="ACHGMT_002669"
     CDS             195..896
                     /locus_tag="ACHGMT_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFN6836364.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1468..2484)
                     /gene="tsaD"
                     /locus_tag="ACHGMT_002670"
     CDS             complement(1468..2484)
                     /gene="tsaD"
                     /locus_tag="ACHGMT_002670"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFN6836365.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2481..2945)
                     /gene="rimI"
                     /locus_tag="ACHGMT_002671"
     CDS             complement(2481..2945)
                     /gene="rimI"
                     /locus_tag="ACHGMT_002671"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFN6836366.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2952..3494)
                     /gene="rimI"
                     /locus_tag="ACHGMT_002672"
     CDS             complement(2952..3494)
                     /gene="rimI"
                     /locus_tag="ACHGMT_002672"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFN6836367.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3478..4200)
                     /gene="tsaB"
                     /locus_tag="ACHGMT_002673"
     CDS             complement(3478..4200)
                     /gene="tsaB"
                     /locus_tag="ACHGMT_002673"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFN6836368.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
BASE COUNT         1306 a          928 c          746 g         1414 t
ORIGIN      
        1 ttattgttgt tttttataca caaatcattc gcttcaaaac aaattattca ataccattgt
       61 atatttttta cataataaca aattatattt gtaaagtatt aaaacttgtt tctaatgttg
      121 gtatgttaaa actttacaaa ttctttaaaa aaagtgacaa taaatatagt gaacaataag
      181 gagaggactt tttaatgcgt gttaataaaa tcgattttca cttttcaaaa ctcagatatc
      241 aacctgattt tgaacatttc tcggatgaag agttccaaca gatcaaggaa catactgttc
      301 ttcgttctta taaaaaagga cagatccttt tcgacgatgg tgatcccaga caacggttgt
      361 atatcttgac caaaggactc gttcgtttag aacgttatga tgaaagtgca acttattact
      421 actatgacta tgtgaataca aataaccttt ttcctatgtc aggaatgttt ggagaagaga
      481 cctacactta tacagctcaa gcgatgactg atattgaaac attctacctt cctgtttcct
      541 actatgaaaa attagcacga aaaaatcccg aacagttgat ctatatgata caaaaaattt
      601 ctaaagtagt tgaaatacat gaattgcgta tccaaatcgg attgacctct agcgcctttg
      661 accgggtaaa acaaaattta ttcattttga aagaagaatt gggcgtacca acaaaagatg
      721 gtcatatcac cattccttat ccgatcacct taaaagaatt agcagtgaat agcggcacga
      781 cacgtgaaac tgctggacaa gtaatcaaac aactacgtga ttccggactg ttagattatg
      841 acaaaaagcg ttttactttc tgtaagacag ctactactcc agctatgaga gaataacgcg
      901 aacaaaaata agcagtgacc ctgatattta tcagggtcac tgcttatttt cctccctaaa
      961 acttctgaaa gatccggcag ttttagatca ctgggcaaga ctcaaagatt ttctatgtgt
     1021 gtttcagtta tgtaatattc actgtttgca aaaaatactg atagattaac tagaggaagc
     1081 gtcttgtcct catccgacct agattattcg atattcctcg acaataaacg attaatcagt
     1141 gagggcaatc agaggaacgg ataatgatga ataagctgta aaaaataggg ctttgacaaa
     1201 agcgtgtcac agcccttatt tcgattaaac ggtgtgtacc tgtcaactct tcggtcacaa
     1261 gtcataatgt tcataaacat gaggagctgt tttttgaaat tgttccttgt gaatcagagt
     1321 taaacgacag gcttacgaac tcttgattaa acggcgttca aaagcttgca tacgcggtaa
     1381 taagaacagc caggtcaaaa acatgagaag ccgtttttcc tgggctgttc ttattacttg
     1441 atgcagggca gatttttcac aacctcttta ttcagataaa accaagcttg gatcagcatt
     1501 caactgatag ctagcaaaat ttcttttttg catttccaca tgtgctgctg ctccaatcat
     1561 tgcagcatta tctccacaaa gtcgaagagg gggaatcact agttctacct cagggagttt
     1621 agttgccaaa gcatcctgca atccttcacg caacccttga ttggccgcta cacctccagc
     1681 tacaatcagt tgcttgactg gataattttg acaagctcga agtgttttag taatcaatac
     1741 atcgatcaca cttgcttgaa agctagctgc taaatcattt ttgtctagat cttctccacg
     1801 ctgttgtgca ttatgcacca aattgataaa tgcacttttt aatccgctaa aactgaaatc
     1861 ataattatct tcatggatca tcgctcgtgg aaaatggtaa ttatcttttc cctgatgagc
     1921 tagctgatcg atttcttttc cgcttgggta agaaagacct aatactcttc caactttgtc
     1981 ataagcttct cctgctgcgt catctcttgt ctccccaatg atttcatatg atccatcttc
     2041 ttgcatatag actaattctg tatgtcctcc gctaacaaga agtgccatca atggaaactg
     2101 gaatggttta accagacggg ctgcatagat atgccctgcc atatggttta caggaataag
     2161 cggcaattgg tgtgcccaag caaaagcctt agctgcagaa ataccaatca gcaacgagcc
     2221 gacaagtcct ggtccataag tgacagcgac tgccgataaa tcttctgccg acacaccagc
     2281 ttctaccagt gcatcttcca agcataaggt gatttgttct acgtgatggc ggctagctac
     2341 ttcaggaact acgccaccaa aacgtttatg actgtttatt tgagaagcaa cgatgttaga
     2401 taagatctct gttccatttc taacgaccgc tacactcgtt tcatcacaac ttgattcaat
     2461 cgccagaatc aattcttcat tcatttcatc gtctccgttt ttaactttct gctcataatg
     2521 atcgcatctt ccgtcggatt gcgataatat gatttccgtt tacctaatat acgaaacttt
     2581 tctgattcat aaagttttct tgctgcttca ttagatagac gtacttctaa aaaaacatgc
     2641 agcgaatctt ttatttgttc cgtaatcagt aattgtaaca aatgccgtgc atgccctttt
     2701 ccttgttctg aaactgcaac agcaatattt gtgatctcga cttcatctat tactttcgag
     2761 tatccaataa agccacagat ctggttatct tcaactaaaa gcagataatc agagtgaggc
     2821 tgttggatgt ctgccaaaaa ctgcattttc gtccaaggtg aaccatgtgg ataagcattt
     2881 tcgctaatca tccatacgca ttctgccaag tacgcttcat caaaatcttt ttttctgcaa
     2941 atcaactaat ctcactcatt ttctaaaagt tttatcatat ccaacgcatc ttcttttgtt
     3001 tcagtgtaat atcttttttt gatcgttgtt gcttgaaaac ctaacttccg ataaagtctt
     3061 tgtgcatctt gattactcat acgcacttct aatgacatcg tctcacagcg attcattata
     3121 gcaaagttct cgatttcgtc aataagcagc gaaccgattc gtttacgctg atatgccggt
     3181 cgaacggcaa tattcgtgat atggcaatct aaaccgaacg ctcttgaacc gataaaacca
     3241 atcattcttc cattatcaaa gatacctaga tatagatgag gaagcggtga cttcaattct
     3301 gatataaagg cactttttgt ccatggcaat tcgccattat agacttctcg ttcgacatcc
     3361 agcagctctt tgatatcttc tgtagtcaat tctcttagta cgtaacgacg atcgatttgt
     3421 tcgattgttt tttctgtata aatcatttta tcttgaaaaa agcttctggc atacgctcta
     3481 aattttttca acataacttt cgtctccagg tgtgtgcgtc tccaaccatt tttcttctgc
     3541 ttctacccgt tttaaatagc gaggaagaaa agcttgtttc tctgcaactg gttcagctaa
     3601 agcacctaat tgagccacag ttacaccact tggaatatcc catgctggaa ctgtattaat
     3661 catcgcacta ggcaatattt cctttatttc atagaagaat ttgtgacaat cactcccgac
     3721 gaaataaata gcttcattca gcttttgtag ttgtgtgaac aaatcagaag cagaaatatg
     3781 tacatcttca agtacaattt ctaatctatt ttctttccag cggtatgccc cagcgtatac
     3841 attttttctt cgagcatcaa acatgggcac aataatttta tccacaccga tgcaattagc
     3901 cgcgatggtt ttcagactag atactcctac tagctcttta tctaatgtat aagcaatcgt
     3961 ttttgctgtc gttacaccta tacgaagccc tgtataagaa ccgggaccat cagaaaccgc
     4021 gattcggtca atgtcttttg gacttatttg aagatctgca aacaattggt cgattgcagg
     4081 catcagtgtt acactgtggt tgcgtttgat atttgtttgt atttgtccaa gaatttgttc
     4141 gtcttctacc acaccgatcg ccaaagtttg gtttgcagta tctattccta atgtaatcat
     4201 atatttgatc ctttctgcta tgacattctc gccttccatt gtagcatact tttttattct
     4261 gaggatgaaa ataccaaagg caaaactagt acaatgatac atgattatct gctggcgggc
     4321 aaattttgcc ccttcctgaa acacaaaaaa gctgcggtat cacttcattt ccccacagct
     4381 tttccatctt ttat
//
LOCUS       DAZBSA010000150         4359 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000150 DAZBSA010000000
VERSION     DAZBSA010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.154"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2327)
                     /gene="esp"
                     /locus_tag="ACHGMT_002674"
     CDS             complement(<1..2327)
                     /gene="esp"
                     /locus_tag="ACHGMT_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFN6836369.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
     gene            complement(2944..3378)
                     /locus_tag="ACHGMT_002675"
     CDS             complement(2944..3378)
                     /locus_tag="ACHGMT_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836370.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3395..>4359)
                     /locus_tag="ACHGMT_002676"
     CDS             complement(3395..>4359)
                     /locus_tag="ACHGMT_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFN6836371.1"
                     /translation="FGPFRANNIDKVFFKLKMNNLNVAMADRERLYNSLQNLTLYSLG
                     DIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKTIEQIKIDNLLSQNYDPEIYLFLY
                     ENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLG
                     MDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQ
                     FIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNH
                     TISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
BASE COUNT         1341 a          815 c          601 g         1602 t
ORIGIN      
        1 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
       61 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      121 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      181 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtactt attatagaca
      241 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      301 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      361 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      421 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      481 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      541 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      601 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      661 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      721 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      781 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      841 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      901 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
      961 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1021 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1081 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1141 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1201 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1261 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1321 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1381 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1441 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1501 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1561 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1621 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1681 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1741 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1801 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1861 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1921 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     1981 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2041 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2101 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2161 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2221 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2281 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2341 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2401 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2461 caacaaaaaa agtacatttc ttcatgtttt tttcggatat ttgtgctttt taaccattca
     2521 gcagctgaaa acatataaaa agatatgtat gtattctttc tgaaattaag ttcatttcta
     2581 tgcgatgaag catgttttca aaatattacc ctttcttaga ttaaacttca gagtattcaa
     2641 ataacggaat aaggagcctt atcccgcatt actgaatgag actgtgacat aagtaaaaag
     2701 gtgacttcaa attccttctt cggaatttga aatcaccttc tttataatta taaagtatcg
     2761 gtttcacagc atttctgaat gattcctaga atcttaaacg aatgattgga gttatcaaca
     2821 ttttttcact cgtttttagc gaaacaaaaa atgacatttg tcacagcctc attaaattat
     2881 tgattctttt tctttataat tgaattgtcc atatttaaag tgtctagtac tctacatgaa
     2941 taattaattg ctttcactca aatacttata atctattaac ttgtagttgt ttaacttttc
     3001 tgaaaaagtt agacccacgt aataatcttc ccagctttct ctttcaatta actgatccaa
     3061 tttttgataa tccataaatt catcatgtat caaaaagaat aaataacttg atattttagc
     3121 atctagctta atcagattaa ataatgtttg tttaaaattg ctaccattaa tatgactgat
     3181 agattctcta gcttcttcac ataaactttt tactttatta attactactt tgtcatattc
     3241 atttaacaca ctattattac cattacttag acgctcagat tctacgaaac aactcaaatc
     3301 agaaatttcc caaaaaaatt ttttgttaaa atccaatttc ctcaactcct ccaaactacg
     3361 taaatgatat agtctcataa taaaataaaa cctctcagct aatgttgttg aaattgtcca
     3421 ctaaacctaa ttgatattgc ttaggagata cacctgcgta ctttttgaat actctcgaaa
     3481 agtgtgaaga atctgaaaat cctaaaccta aagaaacttc actgatagta tggtttgatt
     3541 tcaacattat tttagccgta gaaatttttc gcttagagat ataattataa atcgtaatat
     3601 gcatttcatt cttaaagctt tctcttaatt tagtttcact tacagaaaaa taattagcta
     3661 tttcctttac agtaattctt ttatagatat ttaagccgat gaattgaacg acagaagaaa
     3721 ttaagggaga aagatgctct atcttagttt ttccgatttc cttagtataa taaacaatcc
     3781 cagaatccct cacttttaag acctcaggta acgatacagc taattcgttt tcttgtatca
     3841 aagcatctct actttgatac gcttctatta tatccattcc taatgttata gacgtttgtg
     3901 ctaacttttc aaaaacaatg atagaataat ttttttcaga ccgaatcgta tctccactaa
     3961 cacttggaat tattccatta ctcagattaa aaaccatgtt ttctaaattt cgaatatctc
     4021 catttttaac atattccagt attttatttt cataaagaaa cagatatatt tctggatcgt
     4081 aattttgtga taacagatta tctattttga tttgctcgat agttttactt agattttctg
     4141 tatattcaat taacggttca tggaatagat cttctatttt tccagtaaaa aaataatgaa
     4201 ctaaaatcag aatatctcga atgtctccta aagaatacaa agtcaaattt tgcaatgaat
     4261 tatagagtcg ttctctatca gccattgcaa cattcaaatt gttcattttt agtttaaaaa
     4321 aaactttatc aatattattt gctctaaatg gaccaaata
//
LOCUS       DAZBSA010000151         4323 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000151 DAZBSA010000000
VERSION     DAZBSA010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.155"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="ACHGMT_002677"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="ACHGMT_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFN6836372.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="ACHGMT_002678"
     CDS             complement(2020..2922)
                     /locus_tag="ACHGMT_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN6836373.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="ACHGMT_002679"
     CDS             complement(2919..3611)
                     /locus_tag="ACHGMT_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFN6836374.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="ACHGMT_002680"
     CDS             complement(3654..4295)
                     /locus_tag="ACHGMT_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFN6836375.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
BASE COUNT         1325 a          926 c          755 g         1317 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 ttt
//
LOCUS       DAZBSA010000152         4299 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000152 DAZBSA010000000
VERSION     DAZBSA010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.156"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..1108
                     /locus_tag="ACHGMT_002681"
     CDS             134..1108
                     /locus_tag="ACHGMT_002681"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFN6836376.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1090..1551
                     /locus_tag="ACHGMT_002682"
     CDS             1090..1551
                     /locus_tag="ACHGMT_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN6836377.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1601..2065
                     /locus_tag="ACHGMT_002683"
     CDS             1601..2065
                     /locus_tag="ACHGMT_002683"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN6836378.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2079..2828
                     /locus_tag="ACHGMT_002684"
     CDS             2079..2828
                     /locus_tag="ACHGMT_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFN6836379.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2828..3658
                     /locus_tag="ACHGMT_002685"
     CDS             2828..3658
                     /locus_tag="ACHGMT_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN6836380.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3658..>4299
                     /locus_tag="ACHGMT_002686"
     CDS             3658..>4299
                     /locus_tag="ACHGMT_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN6836381.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFG"
BASE COUNT         1479 a          554 c          821 g         1445 t
ORIGIN      
        1 cattttatta tataaaattg ttataaaaaa atttttttga aaaatagtat tgcaaaataa
       61 agcaatttca tagataatag gtattgtaaa gttatatgac tttataatac ttattagagt
      121 gagggataaa gaaatgagac aactacacaa agaacagatt gaaacagcaa tggcagcgtt
      181 aaaggaaaag acaattgatc atgtatattt tacagcatgt gggggttcac aagccgtttt
      241 aatggctggt caatatctat ttgataaaga attaactgta ccttgtcatg tttatactgc
      301 taacgaattt aactatgcta taccaaaagg attatctgaa aattctttag ttatcacttg
      361 ttcccattct ggcacaacac cagaaactgt agaagcaaca aaaaaagcaa gagaatttgg
      421 agcaatgact atttgtttat ctaatgaaga aggatctcct ctatgggaag cagctgaata
      481 tcctgttcac tatgattggg gtaaagatgt tgatgctagt gatttaaata aaggaattct
      541 ttatggttta ttatttaata ttttacttgt tttagatggc tctaaaaaat gggaaacttg
      601 cttgaatgaa tttgataagt taaatgatct tgttattgct gaaaagagca aatttgaaat
      661 aactgctaaa gaatggggta aaaaccaaaa acgagataaa attatttaca caattggtag
      721 cggtatcaat tacggagaaa tttactccac agcagcttgc tggttcatgg aaatgcaatg
      781 gattaattct aatgctattc attctgggga atttttccat ggtccttttg aaatcactga
      841 ttatgatgta ccattcatct tagtgaaaag tattggagat acaagatttt tagatcaacg
      901 ggtagaagac ttcactaaaa aattcagtaa taaattaatg gttttagacc aagctgattt
      961 tgacttagaa tctatagata gtgtagcgaa agaatatatt gcagctgtac ttagtggcgt
     1021 tgttattcgc caaatggtag aagcgattgc ttttgaaaga ggccattcct tagatgttag
     1081 acgctatatg tggcaaatga aatattaatt aataggaggt tgcaaaagat ggcaaatcaa
     1141 ttatttcaca ttgtgattac aacacattct accctttgtg agggttattt aaaggcaaca
     1201 aaattaattt taatgaatga cgccacaggt attagtgctg ttccatttaa agaaaatact
     1261 agtccagatg attttgagga gagtatgaaa gaaataatta gtcagcatca tttgcaacct
     1321 ctaattattt taacggattt aattggagga acacctaata atgtttctat taaacagttg
     1381 aacaacaata gaattcaaat tattgctggg attaatttac ctcttttact ggagttatta
     1441 atggcccagt cagctggtaa atctatagaa gaaatagatt ttaaggaatt actgaataac
     1501 tctcaaaaaa gtatggttca tattaattcg cttttaaaag atgtcagtta aggtggcaat
     1561 ttctattcta taaattttaa aaattgaagg aggaaataga atgattaaat tagtgcgtgt
     1621 agaccacaga ttacttcatg gacaagtaat attttcttgg actaaacagt atgatattaa
     1681 ccatattatt gtggctgata acaaagttcc aggagatcca atgtctgtga tggctttgac
     1741 aattgctaaa cctgctgatt gcaaattaga cattgttaaa attagtgatg ttaaagggat
     1801 tatggaaaca tccccaaatg ataattacat gattttaatt aaaggaccta aggaagcttt
     1861 tgaattagtt caaaatttac cagaaattaa agaaattaat tttggcggtg tgtctaagaa
     1921 aagcgagtcc aaacaatatg gaaaggcagt ctttttaact cctgaagaat tagaagccac
     1981 aaaaaaaatt cttgatcaag aagtgaaaat atttgtgcaa caagtcccat cttcaagtat
     2041 tgaaaatgta gattttagta agtaaggggg gaaaataaat gttattaaaa gccattttat
     2101 taggtttagt tggtgtggta gctgttattg actccaggtt aattggtcga caaaatattg
     2161 gtagaccact aattttgagt acacttgccg gacttgtgct aggtgacttg catacaggga
     2221 ttgttcttgg ggcatcttta gaattaattt ctatgggatt cgtctcaatt ggggcggctg
     2281 gacctcctaa tatgcaactt ggtagtatta ttgccactgc ttttgcaatt ttaactaata
     2341 gtagtacaga aacagcctta acaatagcta tcccagtggc tgtagctggt gaatttatga
     2401 gtattatcat gcgcatgata attgctcaat ttggacatgc tgcagataaa gccattgaaa
     2461 ataataaatt tagaaaagct caaattattc atatttattg gtcatttatt tttaatgctt
     2521 ttgtttactt tattccaatc tttttaacag tatattttgg tgctgacgtt gtggcagatt
     2581 tagtagataa aattccagaa atgattacag acgccttgac tgtctctgga aatatgcttt
     2641 ccgcattagg gtttgcaatg ctattaagca caatgttgag taaaaaatta tatccttact
     2701 ttatttttgg attctttatt gttgcctatt caggtctttc actaattggc gtgacaattt
     2761 ttgcagctct gattgccttt gtaatggatc aagttaaata tggaaaaaga gaagaggcgc
     2821 atggttaatg gaaaatagta cagaaaaaaa acgtttaatc acaaaaaaag atcttaataa
     2881 gatgtgtctt cgttcatgga caatcatgtg ttcgtggaat tatgaaagac aaatgcatat
     2941 gggatttatg tatgggatgg cgccaatttt aaataaagtt tataaaaatg atgaagagaa
     3001 aaaaagagaa gcttatactc gtcatatggc attttttaac tgtacgcctc aaatgacttc
     3061 atttattatg ggactttgtg cttctatgga agaacaaaat gctcaagcag aagaaggaaa
     3121 ctttaatact gaatctatct ccatgattaa aaccagttta atgggtcctt ttgctggaat
     3181 tggtgattca tttttccaag gtactttaag aattattaca tttgggattg gattgtcttt
     3241 tgctaagcaa ggtagtattt taggaccgat tttagctgtt ggcctttttg ctataccttc
     3301 tattttagct gttttttacg gcggtaaatt aggatatgag tctggcaata aataccttac
     3361 taaattgtat caagaaggtc ttatggatcg agtaatgcaa tttgcttcta tcattggttt
     3421 ggctgtagta ggtggaatgg tggctagcat ggtgccaatt gtcacccctt taaaattcac
     3481 tacaggggga acagaacttg ttttacaaga cttacttgat tccattattc ctggattatt
     3541 accacttgta tgtactttgg gaatatataa attgattaag aaaaatgtta gtacaaatat
     3601 gctcttatta ggttgtattt tggcagggtt tgttttaagt ataataggta tcttataatg
     3661 attaaggctc taatttttga tatggacgga gttttaattg atagcgagat tatgtatcaa
     3721 aagtgggtat ttaatttttt aaaacaagaa aatttaagct atcctttgta ttcttatcaa
     3781 aaaaagattg gaactacagc aagtattttt gatgatatag aaaagtacaa tgagggatgt
     3841 aacagtttag aattgaaggg aaaatttact aaatattgga aatcgcaaaa gattgattac
     3901 ttggatattt ttcctaatat actaattgaa cagcttgaat ggttaaaaaa gaaaaaaata
     3961 aaaatggcaa ttgcttcttc cagtccaatg gatactataa atgaagttgt taccacttgt
     4021 aaaatagagt catattttga ttgtttaatt agcggaagag atttgccaga aagtaaacct
     4081 aatcctacta tattccttaa agcttcagaa cagctcagaa ttccagttga agaatgtctg
     4141 gtgatagaag attcttataa tggtataaaa gctggtaaaa gagcaaatat gaaggttctt
     4201 gcaattaaag ataaacgttt ttctcaagat gtaagtctag cagatgatgt tatttacgat
     4261 attaaatatc tgcgacaaga agtacaggtg aaatttgga
//
LOCUS       DAZBSA010000153         4292 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000153 DAZBSA010000000
VERSION     DAZBSA010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.157"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..579
                     /locus_tag="ACHGMT_002687"
     CDS             127..579
                     /locus_tag="ACHGMT_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836382.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="ACHGMT_002688"
     CDS             593..1282
                     /locus_tag="ACHGMT_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836383.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="ACHGMT_002689"
     CDS             1407..3758
                     /locus_tag="ACHGMT_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFN6836384.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="ACHGMT_002690"
     CDS             3838..4233
                     /locus_tag="ACHGMT_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836385.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
BASE COUNT         1506 a          543 c          860 g         1383 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa ta
//
LOCUS       DAZBSA010000154         4277 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000154 DAZBSA010000000
VERSION     DAZBSA010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.158"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(30..515)
                     /locus_tag="ACHGMT_002691"
     CDS             complement(30..515)
                     /locus_tag="ACHGMT_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HFN6836386.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(517..1188)
                     /locus_tag="ACHGMT_002692"
     CDS             complement(517..1188)
                     /locus_tag="ACHGMT_002692"
                     /inference="COORDINATES: protein motif:HMM:NF037286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836387.1"
                     /translation="MSRFSINVVEVDDYSEITDILKSIRKKYLRKTIFISGSAVDYTP
                     FTSESKGLKFVEKLAYRLSESGYRIVSGYGLGIGNSIVSGVLKQRRNLRKNNIQDVLS
                     LRPLPLDMPHEWRRYRENIIAESGISIFVFGNKLESGEIVTADGMIEEFELSVQNENI
                     VVPIGFTKGASKVLFDKIQENFSDYFDDSLKSKFQALEKLDTEGEVDKKVEQIVSLIN
                     SIQED"
     gene            complement(1451..2644)
                     /locus_tag="ACHGMT_002693"
     CDS             complement(1451..2644)
                     /locus_tag="ACHGMT_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6836388.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(2728..2928)
                     /locus_tag="ACHGMT_002694"
     CDS             complement(2728..2928)
                     /locus_tag="ACHGMT_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFN6836389.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            complement(3352..3585)
                     /locus_tag="ACHGMT_002695"
     CDS             complement(3352..3585)
                     /locus_tag="ACHGMT_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6836390.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(3592..4023)
                     /locus_tag="ACHGMT_002696"
     CDS             complement(3592..4023)
                     /locus_tag="ACHGMT_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFN6836391.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         1054 a         1067 c          832 g         1324 t
ORIGIN      
        1 gtcattagat agtagacagt tccaactctt tatttacctt ttttgtatgc tgcactgtca
       61 atccaagttc ctaaattgtt gtatccatca tcacttatat aatcatattg gccatcaata
      121 atttcgtcaa accacaaata ttctccatct ggtttttgcc caattatttt aattactgat
      181 tgactagtag ttgtcatctg gttaaaatct tttattttac ctatattaac ggccaaaata
      241 gcatttcctc gcttataact ctcctggatt tcatatttaa catattctcg atcaagagtt
      301 tctttaccta tcagcaccac agtcactgat gttccaatta attgattatc tatccagttt
      361 tttatagcaa tatctccatt acgtttaact ttctcaaatt cagccttatc tacaaaacca
      421 gcttcctttg ttcctcgcaa agtccagctg tttctaacta ccattgctct tgttacatct
      481 gcataatgga aactaaaaaa aacccttctc accatattaa tcctcctgaa tactatttat
      541 taaactgaca atttgttcaa cttttttatc tacttctcct tcagtatcta atttttctag
      601 cgcttgaaat ttacttttta aggaatcatc aaaataatca ctgaaattct cttgaatctt
      661 atcaaacaaa accttacttg cacctttggt aaatccaatc ggaactacta tattttcatt
      721 ttgaacagat aattcaaatt cttcaatcat tccatctgct gtaactattt cacctgattc
      781 aagtttgttt ccaaaaacaa aaatactaat cccagactct gcaataatgt tttctcgata
      841 tcttctccac tcatgaggca tatcaagggg aagtggtctt aaagaaagta cgtcttgtat
      901 gttatttttt cttaaatttc ttctctgctt tagaactcct gaaacaatac tattaccaat
      961 tccaagtccg taacctgata ctattctata accggattca gaaagcctat atgctaattt
     1021 ctcgacaaat tttaatcctt tcgattcaga tgtaaatgga gtataatcaa cagcacttcc
     1081 cgaaataaaa atagtttttc tcaaatactt ttttctaata cttttcaaaa tatcagtaat
     1141 ctcagaataa tcatctactt caacaacgtt tatcgaaaat ctggacaaat cattgatttg
     1201 tagtcttctt tttgtcagtt cataattata tctctcttga tcaaaaaaat cctcagaatc
     1261 gacaaaattt tcctttaaaa ttatatggga ttttagatta tctttttaga agttcttgaa
     1321 tgtcggtata gcccgtgttt ccgggctttt ccggcatttt tgatattgtt agatacttac
     1381 aaatgttctt attacctctc accatttccc cctcaaaagt agtaaatgga gtagtaaaac
     1441 cggccttgtg tcaggctgcc agccgttcca tttctgccct tgcggaatcg aatgtggcgt
     1501 gtgcataata gttcagcgtc atggtgatat tggaatgtcc cataatgtac tgcaaggctt
     1561 tggggttcat tcccgcattg gctaaccggg tgcagaatgt atgacgcata gcatggggcg
     1621 tcgtcgtttt cggcaacgct tcttcatggc tcttgttgta cttcttcaca agcctgccaa
     1681 acatcccgcc ataatctacc gccgtcatgg gatagccgtt ctgtttgaga aacaggaaat
     1741 tggcataccc gtcaataatg aacggctttg cacctttgcg gttttttatc actcgctgga
     1801 acgcctgata cacttcctca ttcatgggga tttttctgac gccgctttgg gtcttgggtt
     1861 cgtcaatata gtaaccgacg ccggtatttc tcaaaagctg gtggctcacg atgattaccc
     1921 ggttttcaaa atccaaatct ttgtccgtca gtccgcacag ttccgaaatg cgaagccctg
     1981 tccccagcag aataacgatt gcgtcataat gcttttggta aaccttatca ccttgaataa
     2041 aggacagaaa actttcttcc tgtgctggtg taagaggcac ctttggttcc gtatcgtcgt
     2101 ccagaacgtc actaagctgg aagtcaaacg ggttttttcg gatgcaatcg tcctgtatgg
     2161 cggtgtaaaa agccgctttc agggaacgct tgtcgttatt gatggtctta taagaaaggc
     2221 ccttttcttt catccgaagc gcccattcct ttgcgtcgga cagcttcaca ctgtcaatcg
     2281 ggcagctccc cagccgatct tgttccagaa tacgcatcag ccgttcccgg cccagtttgg
     2341 tgttgtgctt cacattgccc cggtgcctga tctgcttggc gtagagctgg cagacggtca
     2401 ttttcttgcc gatggtgtct atcccgtcgt caaggtcttt ctggatttct ttctcctttt
     2461 cccggagtga aatatcctcc cgtttacctg ccggggtctt atccgtaggc actaacttcc
     2521 atgagtaaac aaactgcggt tttccaaagg tatcaacata tttgtaagca tatcgtccgt
     2581 cttttctctg gctctctcca gaccgcaaaa cgcgattttt gctgtctcgt cttttttctg
     2641 acatctcgtc atgctccttt ccatgacgga aagagccgca tatatgctta taaattatta
     2701 taacatatct gcggcccttt ttcactatta gattgtgtcc aaagtgtcaa ttattttctc
     2761 aaactgcttg cgtttgatct ggatgcggtt gccattcata atgacccagc cagaggccgg
     2821 attttcctcg gccagcttac gcaatttgtt ttctccgata cggaaatatt tagacgcttc
     2881 ctcaatcgtc agcgtgtatt tttcccaaat aggcacatca tggttcataa tgcagccccc
     2941 tttcacaaat tcaaaggttt gtgtgattta ggggcataga cggacggctg ctggcacagc
     3001 tccacgggac tttcaccccc gcgtggttct cacgcggccc tacccattgc ctgcgacgct
     3061 tttaacgctc ggactgtggc tataagggag tatcatagtc ctgcctgtac gtcgtcgccc
     3121 gcaagccgcc acgctcacat tgcggcgtgg tgttcctcgc tccgcttttc ccgtagggaa
     3181 aagtatcatg gcgcaccagc cgtccggctc ggcacagaca gaatgtgtcc gtatgcccta
     3241 tgatgcgccg ccgttatctt gcgggcatat ttatcaaggt gcgatcaggc caccgggaaa
     3301 acaccgaaac ggggaaaggg taagccgttt catgctgccg gggccggttc gtcagttcgt
     3361 gacgatagcg tggatgagct tgatttccag acggcgcttc atggcctcgt ccagacacac
     3421 atgcggatag ccgttttcgt catagagcgt ccgggtacac agcttgttca tgtaaccgct
     3481 gtaatactgc aaaacgcgct ctacggctcc ggcatcccca gcgcgggcgg cctcgatcac
     3541 cgaaaggggc aaaagctcaa tgcccttgta gttcgggttc ttcatccgcg tctaacctcc
     3601 cttcggcatc aacgccttta gctgtttccg cagctccgat aaggtatttg tcctgtgccg
     3661 ctgaacggca ctccgggaca ttcccacaag gccgccgatc tcaccatctg ccatatccag
     3721 aacacagtgc aggattaaaa tactctgctc catgctgggc agcgcggcaa aggcttcggc
     3781 aacaagctca ttgttgatat gtaggtcata gccatgcgac gaaaacgtaa agctgtcgct
     3841 ggggtagtga tctactgtac agagcttgtc caaatctgcc tgtgacaaag agcttagtga
     3901 cacttcacgg tcacggcggc gcttcatttc agccaagtag cttttggcct cgttacgcag
     3961 taccgtcttg caaaaagcat cgaacatgca ccgcgcttca tagtcgcggg ggatagcatc
     4021 catcttctca ccccctttct gtgggggact taggtggttc tctccctttc cgctaacaag
     4081 actttgacaa ggccgccagc tacccgctaa atacctcctt tttgaaaatt tctcaaaaaa
     4141 tttttttggg gacagccggg ccgcaaaaga cgacaaaaat cgcccggcag gtcgcaaacc
     4201 taccgggcga gaaaatgctt tgctatgctg ctgatttaca tggtatagta catactcata
     4261 accggaatag ccctgtg
//
LOCUS       DAZBSA010000155         4126 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000155 DAZBSA010000000
VERSION     DAZBSA010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.159"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..427
                     /locus_tag="ACHGMT_002697"
     CDS             <1..427
                     /locus_tag="ACHGMT_002697"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFN6836392.1"
                     /translation="VIMACFSGMTTNIGNIFAVYNKTNQLLMISVVSGLLSVVGTYLL
                     GYSFGVIGVSFATLLGTLVLFGLRYYFGRKIAYYAFDWKRISLLLVLYSAIAFVCIHR
                     GFYFNLFLTIIFSAIFVFTYKQLIITYLKKFFRIKMAKR"
     gene            513..941
                     /locus_tag="ACHGMT_002698"
     CDS             513..941
                     /locus_tag="ACHGMT_002698"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFN6836393.1"
                     /translation="MIFGKKHSLVEVPEFGSEPYLIEIGDNFRCSFGVKFITHDGGVW
                     VLRKMSSVSESTSKIGRIKLGNNIFIGIYSIVFPGVSIGDNVIIRANTLVNKDIPNNS
                     VVAGSSCKVISTIEEHYEKNKNEFIKYRTSMILKQKNFFY"
     gene            1029..2195
                     /locus_tag="ACHGMT_002699"
     CDS             1029..2195
                     /locus_tag="ACHGMT_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFN6836394.1"
                     /translation="MKISVFGLGYVGLANSLLLAQKEQVKAYDIVEEKITMLQNGISP
                     IEDKEVHEFLKRDDLNVEFTSNFTDAVNFGDYLIIATPTDYDEKKNYFNTSTVEDVIE
                     KALAIRPDSTFIIKSTVPVGYANEIKAKFGTENIIFSPEFLREGKALYDNLYPSRIIV
                     GECSARGREIATMFQRNAFNKDASVLLTHSTEAEAIKLFSNTYLALRVAYFNELDTYA
                     EFRGLDSKQIIEGVGLDPRIGSHYNNPSFGYGGYCLPKDTKQLRANYEKVPSNIISAI
                     VDANTTRKDFIAGQILAKKPNVVGIYRLTMKAGSDNFRYSSIQGIMKRIKAKGIEVVV
                     YEPTLDEDEFYHSQVLKDINEFKNISDVIVSNRFEDILKDVEEKVYTRDIFGDN"
     gene            3411..3530
                     /locus_tag="ACHGMT_002700"
                     /pseudo
     CDS             3411..3530
                     /locus_tag="ACHGMT_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
     gene            complement(3578..3739)
                     /locus_tag="ACHGMT_002701"
                     /pseudo
     CDS             complement(3578..3739)
                     /locus_tag="ACHGMT_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3871..>4126)
                     /locus_tag="ACHGMT_002702"
     CDS             complement(3871..>4126)
                     /locus_tag="ACHGMT_002702"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836395.1"
                     /translation="FCIKKYRKDTLKSFRINLPNKFIGGFKMSHTLLIKDTLNILDLN
                     INIHFEENCLTKEKYKGQICLIYKGTLQYTPEEYPHLPIY"
BASE COUNT         1384 a          536 c          791 g         1415 t
ORIGIN      
        1 agttattatg gcatgtttca gtgggatgac aacaaatatc ggaaatatat ttgcagtata
       61 caataaaaca aatcagttat taatgatttc agtagtgtca ggtctattga gtgttgttgg
      121 cacctattta ttaggctatt cttttggagt aattggtgtc tcatttgcta ctttgctcgg
      181 tactttagta ttatttggac ttagatatta ttttggaagg aaaattgctt attatgcatt
      241 tgattggaag cgaatatcgc tacttttagt tctctattcc gcaatcgctt ttgtatgcat
      301 tcatagaggc ttctatttta atcttttctt aacgattatt tttagtgcta ttttcgtttt
      361 tacgtataaa caattaatta taacgtatct aaaaaaattt ttcagaataa aaatggctaa
      421 aaggtgaggg tatacgaagt aagaatagaa tctttcgaag aataaagaga aaactatttg
      481 gaaaaaatct gcgagaatgt attaatgaag gtttgatatt cggaaaaaaa catagtttag
      541 tagaggttcc tgaatttggg agcgagccat atttaattga aataggggat aatttcagat
      601 gttcatttgg tgttaaattt ataacacacg atggtggagt ctgggtctta aggaagatga
      661 gttctgtgag cgagtcaact tcaaaaatag gtaggatcaa attgggaaat aatattttta
      721 ttggtattta ctctattgtt ttcccaggag tttcaattgg cgataatgta attattagag
      781 cgaacacttt agtaaataaa gatatcccaa ataatagtgt tgtagcgggt agttcatgta
      841 aagtgatttc aactatagag gagcattatg agaaaaataa aaatgaattt attaaatatc
      901 gaacctcgat gattttgaag caaaaaaatt ttttctatta aataatattg attgaataag
      961 gaatatgttc aaaaggataa cagctaaaat actataaaat gaaatttgat tggagcgtat
     1021 ggaagaatat gaaaatttca gttttcggat taggctatgt tggtctagca aattcactat
     1081 tattagcaca aaaagagcag gtcaaagctt atgatatcgt ggaagaaaaa atcacgatgt
     1141 tacaaaatgg catctcacca atagaagata aagaagttca tgaattttta aaacgggatg
     1201 acttaaatgt cgaatttacg tcaaatttta ctgatgcagt aaacttcggt gattacttga
     1261 ttattgcaac tccaacagat tatgacgaaa agaaaaacta ttttaataca tcaacagtag
     1321 aagatgtaat tgagaaggct ttagcaattc gtccagattc aacattcatt attaagtcga
     1381 cagttccagt tggctatgca aatgaaatta aagcgaaatt tggtacagaa aacatcattt
     1441 tttcacctga atttttgcgt gaaggaaaag ctttgtacga caacttatat ccatcaagaa
     1501 ttatagttgg agaatgttct gctcgtggtc gcgagattgc aactatgttt caaagaaacg
     1561 catttaacaa agatgcatca gttcttttga ctcactcaac tgaagcagaa gctattaaat
     1621 tattctcgaa tacatatttg gcattgcgag tggcgtattt caatgaatta gatacgtatg
     1681 cagaatttcg tgggcttgat agtaagcaaa ttattgaagg tgtcggtttg gatccacgga
     1741 tcggttcgca ttataataat ccttcttttg gctatggtgg atactgttta cccaaagaca
     1801 caaaacaatt aagagccaac tatgaaaaag taccaagcaa tatcatcagt gcaattgttg
     1861 atgcaaatac aacaaggaaa gattttattg ctggacagat tttggcgaaa aaaccaaatg
     1921 ttgttggaat ctatcgttta acgatgaagg ccggttcaga taattttcga tattcgtcga
     1981 tccaaggaat tatgaaacgg attaaagcca aaggaattga agtggttgtt tacgagccta
     2041 cattggatga agatgaattt tatcattcac aagtattgaa agatattaat gaatttaaaa
     2101 atatttctga tgtgatcgta tctaatcggt ttgaagatat tttaaaggat gttgaagaaa
     2161 aagtttatac cagagatatc tttggtgata actaaataat aatgaacggg tacatggagg
     2221 aaaatatttt agatggtgac tcgaaataaa tgtttgttct cattgcatct gtatctatac
     2281 cgataagttt aaaaattccg attaggaaaa tgaagttggc tatccttgcg aggaatttat
     2341 tgctggatta ttttatgaac taaagcaaga catcatcaga aaaaaatcca aaatttgaca
     2401 atgggattga tcgtttacca gctgtaagcg aagtatctga cacgaaaatg gaaaatgcaa
     2461 ttactgttat taccaatagc tttttttcat cgatcaagta tttgaaaaca ttatttgttt
     2521 gccaagctga tcaatgttct ttcagacttg gaagaacatt gatcagctca caagtaatga
     2581 ttgaagttta ggttatggga taaaggataa gtgaaataaa agtcgtgtct gcatctttgt
     2641 tagtaccagt aaatttttct actacggcgc tagtcacgac agtccaggca tctgaaattg
     2701 aagatgcaaa tagagtttct gctagtttag gtattgatgt tactgtatta gcattaatct
     2761 tagagccagt agcatccaat aggtgtagct atagttctaa tagtataaaa caatgcagag
     2821 gatatgattg ctttatgtct cggtgtgtca ggtcaaaata gagttccata tggggcgatt
     2881 tctcaatgaa taatcttata gatataatct tgtttgctat ttcgttatca cttttacttt
     2941 tcttcgatta tttcaaaaat aaaaatccaa aatattttaa gttcttacta tttatcatta
     3001 ctgtactagc tgttttatcg atgacagatt ttatttaact tatctcaaaa agtagggaac
     3061 catatcattt ttcagtaaca tgcgtattat ttgtataaat aatacaaaat gaaaaattat
     3121 gattattcta ttgtcctttt agaaagcgtt ttctgtgagc ttttaatcta aaaggaggga
     3181 tataaaataa agaaaaccta ttttatgcta tttttttgtt gtttctgtgt aggaatcctg
     3241 tggtatatag gtggtaatga aaagaagatt catggagttg tgacaaatat ttctgcggat
     3301 gagttcgtat ttgaagatga aaaacagaac gaatatagta ttcaaaaaag aagctctttg
     3361 tcaaatgatg ttggtaaaga gaaatcttag aataatttgg acagaagagg ctgggacaga
     3421 agtgtttaac tccgagaaat aagaagaaat tcacgaaaat tgcttttcaa atttttgtga
     3481 atttcggctt atttccgaag gagttgcttc tgctcccgcc gtttactcga gagtctgtag
     3541 caaaactgat ttttagattt atcccacatt cttctgttta tttggttaaa aatgttttgc
     3601 catgacgaat catgatccgt agtttgaaat tgtaaaagct gcgaaagcca tacgtattgc
     3661 gttttaatac tttgatgtgg ttgtttaaac attccaatgg tctgtttgaa tagctgcagt
     3721 taagcgcatt ttgaatctgt ggcagaaaat ttttaaacgt tcgtaaagtg gttgtatagg
     3781 tttcaggcag atcgctaatg tctctagtta aacagtcctc aaatagtgac gaatctcttt
     3841 tctttcactc aatgattcaa gaaggttctt ttaatatatt ggaagatgtg gatattcttc
     3901 tggtgtgtac tgaagagtcc ctttgtaaat caagcaaatt tgtcctttgt atttttcttt
     3961 agttaaacaa ttttcctcga aatggatatt gatatttagg tctagaatat ttagggtatc
     4021 tttgataagc agagtatgag acatcttgaa tcctcctatg aatttgttgg gcaaattaat
     4081 tctaaaggat ttcagggtgt cttttctata tttttttata caaaaa
//
LOCUS       DAZBSA010000156         4071 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000156 DAZBSA010000000
VERSION     DAZBSA010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.160"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..1434
                     /locus_tag="ACHGMT_002703"
     CDS             85..1434
                     /locus_tag="ACHGMT_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN6836396.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1562..2152)
                     /locus_tag="ACHGMT_002704"
     CDS             complement(1562..2152)
                     /locus_tag="ACHGMT_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFN6836397.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2299..3651
                     /gene="brnQ"
                     /locus_tag="ACHGMT_002705"
     CDS             2299..3651
                     /gene="brnQ"
                     /locus_tag="ACHGMT_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFN6836398.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1175 a          745 c          789 g         1362 t
ORIGIN      
        1 aaattttgga atcttagttg tgtttttcag aaaactctct taatatggaa gtaattaaac
       61 ttttttagtt aggatgattt aaaaatgaat gaagagcaag aactgcagcg tggactgaaa
      121 aatagacatg tccaactgat ttctattggc ggagcgattg gaacggggtt gtttttaggt
      181 tccggaaagt ctatccagct agctggccct tcgatattgc tcgcctatct tattactgga
      241 tgcatctgtt ttttcattat gcgtgcgtta ggcgaacttc tgttatcaaa caccaataac
      301 cattcttttc tggattttgt tgcagaatat cttggcaaaa aagcggcttt catcactggc
      361 tggacgtatt ggttctgctg ggtatcgatt gcaatggccg atttaacagc catcggaatg
      421 tatgtacgct actgggcgcc tggtgttcct cagtggcttc ctgaactgat tgcattaggt
      481 cttcttcttt gcttgaatat ggtggctgtc tcattgtttg gtgagttaga gttttggttt
      541 gcattgatca aagttgtagc gatcattgcc tttatcctcg taggagctta tatgatcctc
      601 acccactata cgacagatat tggcacagcc agtatcacga atctatggtc tcatggcgga
      661 ttctttccaa ctggtgcaaa aggatttatc ttagctttcc aaatggtaac ctttgctttt
      721 gctgggatag aattagttgg attagtcgct ggagaaacag aaaatcctga aaaagtgttg
      781 ccggaagcta tcaataatat tcctatcagg atcatcttat tttacttagg atctcttttt
      841 gtcattatgg caatctatcc atggaacagc ttgaatccag ataacagtcc atttgtagaa
      901 gtattttcgg aaattgggat cacgattgct gcttccttga tcaatttagt cgtattaagt
      961 gcagcagctt ctgcctgcaa cagtgcaata tatagtacag gacgtatgct tcgttcttta
     1021 gcgcaagaag gcagtgcccc taaaaaattc aaaaagttaa caacacatca tgtgccaggg
     1081 aatgctttga cattttctac tatcgtcatt ttcatttctg ttatcttgaa ttacgtgatg
     1141 cctagtgaag tattcacact agtaagcagt attgccacta cttgtttctt gtttatctgg
     1201 agtatgcttg tctacacgca catgaaatat cgtaaaagta tcctcggcaa aaaagctcat
     1261 tcatttaaaa tgccgttgta cccatttagt aattatctgg tatttcttta catggccttt
     1321 gtgtgtgtcg tcttgttcct aggaaaagat acgagaatcg ctttattact aacacctgtg
     1381 tggttcttgc ttctattact aatctatcat atgaaatatg aacgtaagaa gtaagaaagt
     1441 agttgtgaca gaagtggtca gcttcgagaa ataagaagga atttacgaaa attgcttctt
     1501 aaatttttgt gaaatttcgg cttatttctt ggagataaaa gcttctgtcc cagcctcttt
     1561 tctatttccc cggaaacaac cgctgataat aggcatctgc ttttttatag gaaaaaatcc
     1621 aaataccaga tacaaaaaaa cgtccgaata acccgatcaa cggtacaaaa aagacaccgg
     1681 caatcaagca taagttaatc atcatggaga taagacttcg acggtccttt gacaaaactc
     1741 tcacgacatg ggaatctggt ttatttgctt tcatgatttg cctcatcaag ataccaaaag
     1801 aaaaactcca catcgtgtat actaagacat aagccatctc aggccaaaaa gcttcagggt
     1861 tacgcccgac ccaactagta acagaaggac ataaagtcag ccaaaagagc aaaaagatat
     1921 tcgaccagac cactcggtaa ttaattcgtt ctgccatctg aaatatacga tgatggttca
     1981 cccagatcac cgcaacaaat ataaagctga tggtataagc taataatgta tttcggatga
     2041 cccataaaga atgaaagcta gatgtattag ggatcttgat ctctaataca agaactctca
     2101 aaacaatggc taccactcca tcagtaaatg cttctaatct cgactttgtc acgatgattc
     2161 ctcctcagat gattcagcgc ttttatttta tcacttacgt tattaaataa ccatcgtttt
     2221 atatagggaa ttttctgaaa attgttgcat atttttcgaa attttgttac ttttaaaaaa
     2281 accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct ttttataggc
     2341 tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt tcatttgggt
     2401 caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac cggtatcggt
     2461 cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt atatgaatta
     2521 gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta cttagttatc
     2581 ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg tttagcacca
     2641 tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt attttttgtg
     2701 ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg aaagttcctg
     2761 aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt gaatccacta
     2821 ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt tttcacaggg
     2881 tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg gatcatcatt
     2941 gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa agacacgatc
     3001 aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct cgcttattta
     3061 ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac gctggctcag
     3121 attgctgatc attatttagg tacttatggc agtatcttat tagcatttat cgttatatta
     3181 gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt cacagatttg
     3241 tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc ttgtttagcg
     3301 gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat gttcatctat
     3361 ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa gcagcgtacg
     3421 gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga tgggttgaac
     3481 gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata tgttgcggaa
     3541 agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt tatcggtttt
     3601 gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg ataaaagagg
     3661 ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat ggcttctcac
     3721 atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg tttattgaga
     3781 ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa atggcttctc
     3841 acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac cgtttattga
     3901 gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct ccatatactt
     3961 tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt tcttttataa
     4021 tatagatttt agacatatat atgatataaa aatagttttc gcaaaaaaac t
//
LOCUS       DAZBSA010000157         3972 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000157 DAZBSA010000000
VERSION     DAZBSA010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.161"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(81..1586)
                     /locus_tag="ACHGMT_002706"
     CDS             complement(81..1586)
                     /locus_tag="ACHGMT_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN6836399.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDPFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1583..2269)
                     /locus_tag="ACHGMT_002707"
     CDS             complement(1583..2269)
                     /locus_tag="ACHGMT_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN6836400.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2462..3883)
                     /gene="gndA"
                     /locus_tag="ACHGMT_002708"
     CDS             complement(2462..3883)
                     /gene="gndA"
                     /locus_tag="ACHGMT_002708"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFN6836401.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1091 a          848 c          684 g         1349 t
ORIGIN      
        1 catccctgaa tttctcactt gcttgtattt tgtaccttat tctttgttgg atatataaaa
       61 aaacaagtaa agcacatgct ttacttgtct tctgcctttg cgataggaat aaagattcta
      121 aaaattgtcc cttgccctac tacactttct gctaagatac gtcctttata gttttcaacc
      181 agttgtttag cgatagacaa ccccagacca tttccaccct tcgttctagc tcttgcttta
      241 tccactcgat aaaaacgatt gaagatctta ttaaggtctt ctttgggtat tccttcgcca
      301 aagtcctgaa tcgcgatctc aaattcattc agagtggaag aaatagagat atggacttct
      361 tttcgtgtag ttgaatactt tactgcgtta tccaatatga taatgattaa ttgttcaaag
      421 tgattacgat aaatttgcag ggtcacttct tttgtcaaat catcatctaa agtgatcaca
      481 tagtctggat ataatatctt gaaattatta taaacttgat aggtgacttc ttttccttta
      541 gttgtttcat tcgcataatg aacatctact tgttctgctc ttgataagtc tagcatctct
      601 tgaactaagc ttttcatacg gctgatttct tgcatgctgg cgcttaatga ttcttctaat
      661 atctcaggat catctttccc ccaacgattt agcagactaa gatgcccttc aataattgca
      721 acaggtgtcc ttaactcatg agatacgtct tccacgaatt gctcttgttg ttcaatatac
      781 aaacgcatac ggtcaagcat ctcattgaat atttcagcta aatctgccaa ttcatcattt
      841 gtgtcgattt caggcatgtg gatatctgat tgcggatctt tacgtatagt atccattgta
      901 tctctgagta ccttgagtgg ttttaagaaa taagaagata aaaagaaacc taaaatacta
      961 cttaagatca aagaaacaac ttctaaaaca ataagagtca ataatagctt atttcgaata
     1021 tcatagaaag aagataattc atagaagctt tgcgcatagc cgatcttttc tcttgtacct
     1081 ttagaatata taggctggat agataaaaag ccagtttttc cttctagtgt aacgatcgtt
     1141 ggaactttac gtgtagtctg aatcaggttt cgactgtttt catgggtttt aaaaatcaag
     1201 ttctcgtcca aatcataaac ataaagatcc attgatggct gccctagctc agctataaag
     1261 ggatcgatct tcattaagtt tccttcgacc agtgaagtat gatctacgac ctcatctccg
     1321 tcatttgtcg ctttcgtcag attacgatac gtatttacta atgtcagttc ttcatccgag
     1381 ttagccaaac gagaagctac ttctgaaatc gtacgctcta cattgtttct ttcttttgcc
     1441 acaatcagat tgatagatga tttataggta atgacagcga aaattgtaaa cacaacgaat
     1501 atgaagaaag aactcgcaaa ggcccacttg attgtcaagg agggcccttt cagttctctt
     1561 ttttccgatt ttttcggctt tatcacgagc gcatcacata acctgttccg cgtacagttt
     1621 ggatatagct ttcctccccg ggaacgtcaa ttttgttgcg aagataacga atatatacgt
     1681 ccactacgtt tgtttcaact tctgtttcgt atccccacac tttatttagt aaaacgtcac
     1741 gtgctaatac cacgttcacg ttttccatta aagtaagcag caactcgtat tctctttttg
     1801 ttagttcaat tacttcattt ccgcgacgta cgacacggtt ttctttttcg atagtcaaat
     1861 cgcgataagt aatagtagtt tgtttagcca catttttatc gccttcaata tcgattcgac
     1921 gcaacaatgc acgaagacgt gctaataatt cttcgatagc aaatggtttg acgatgtagt
     1981 catctgctcc gtggtcaagt ccagatacac gatcgatcac agaatctctt gcagtcatca
     2041 taatgattgg tgtatttttg acttggcgta cacgtctgca gacttctaaa ccgttcaatt
     2101 ctgggagcat cagatccaac aatatggcat cccactcatt atttaatgcc gcatctaaac
     2161 cagttcggcc gttgtagtgg acttctgcgt tatacccttc atgcttcaac tctagctcga
     2221 caaatcgtgc caagttcttt tcatcttcaa tgattagaat attgctcatt tattatttta
     2281 tcctttctat tctactggct ttttaaaaac tctatcatta taccgataat atgcttaaaa
     2341 atctatagtc attttatcat ggatagcaat tttaaaaaag agctgaagtc tactcataac
     2401 aatcgacttc agctctttcg tcctcttttt attttctcat ttcctaacag ccgaaagact
     2461 attattcttc atggtaccat gagtaatgga agattccttc tttgtcgaca cgctcatagg
     2521 tatgtgcgcc aaagtaatcg cgttgggctt ggattatatt tgcaggcaga cgttctgctc
     2581 tgtatgaatc gaagtaagcg atggctgatg agaaagtcgg tacaggtacg ccagcttgga
     2641 cagccaaggc aacgacgtct ctgactgatt gctgatattt tttcgtgatt tcaacgaagt
     2701 aatcatctag caataaattt tcgatctcag gatttttgtc ataggcatcc gtaatttttt
     2761 gcaagaatcg agctcggatg atacatccag cgcgccagat ttttgcgatt tcaccaaatg
     2821 gcaagtccca atcaaattct tttgaagcga cacgtaattg agcaaatcct tgggcataac
     2881 tcatgatttt actgaaatac aacgcttctc ggattttttc gaccaattct tttttgtctc
     2941 ccgtgaactc aaaatcgttc gtacgagaca aaatcttgct tgcttgtacg cgttcttctt
     3001 tataagcaga aatatagcgg gcaaatacag attcagtgat caatggaagt ggcacaccta
     3061 agtcaagcgc gctttggctt gtccatttac ctgttccttt attcccagct gcatcaagga
     3121 tcacatctac tactggttta cctgttcctt catcgtcttt acgtgtcaga atgtcagcag
     3181 tgatttctat caaatagctg tctaattcgc cttgattcca ttctttaaag atatcagcca
     3241 tttcttctac tgaaaggttt aaaatattct tcattaagtc ataagattca gcaatcagtt
     3301 gcatatctcc atactcaata ccgttgtgta ccattttcac ataatgtccg gctccatttg
     3361 ggccaatata tgtgacacat ggtgctccgt cttctgcttt tgcagaaatt tgctctaaga
     3421 ttggtgcaac tagcgcatag gcttcttttt gtccaccagg catgattgaa ggtcctttta
     3481 aagctccttc ttcaccaccg gaaacgcctg taccgataaa gttgatgcca gagttggcta
     3541 actcttcatt acgacgcatc gtatctttaa agaatgtatt tcctccgtca atcaaaatat
     3601 cgcctttatc aaggtgagga agtaactctt ggattgttgc atctgttgcc ggtcctgctt
     3661 tgaccattag caagatccga cgtggtttct cgatagattc tacaaattct tcgattgtat
     3721 atgttgcttt gaaattttta tctgggtgtt cttctactac agcggttgtt ttagcccctg
     3781 tacgattgaa taaagccact gaatagccgc ggctttctat gttcaaggct agatttttac
     3841 ccataacggc cataccgaca acgccgattt gttgttttga catatgaact cctcctacat
     3901 acgggatttc tccctaatct cttacattat agcttaacac ggctacattg caaactaaag
     3961 aacaacatat tt
//
LOCUS       DAZBSA010000158         3947 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000158 DAZBSA010000000
VERSION     DAZBSA010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.162"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..691
                     /locus_tag="ACHGMT_002709"
     CDS             <1..691
                     /locus_tag="ACHGMT_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HFN6836402.1"
                     /translation="ADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADI
                     HEVSEAVDCFIDSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDV
                     GICAVAMGAKVIEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAM
                     PSELSTKEVSRKSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPIS
                     ADSVLLEEDVE"
     gene            688..1836
                     /gene="neuC"
                     /locus_tag="ACHGMT_002710"
     CDS             688..1836
                     /gene="neuC"
                     /locus_tag="ACHGMT_002710"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFN6836403.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            1809..2486
                     /locus_tag="ACHGMT_002711"
     CDS             1809..2486
                     /locus_tag="ACHGMT_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFN6836404.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            2437..3618
                     /locus_tag="ACHGMT_002712"
     CDS             2437..3618
                     /locus_tag="ACHGMT_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFN6836405.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            3631..>3947
                     /locus_tag="ACHGMT_002713"
     CDS             3631..>3947
                     /locus_tag="ACHGMT_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN6836406.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVA"
BASE COUNT         1433 a          513 c          789 g         1212 t
ORIGIN      
        1 tgcagacttt ctaaaacaaa ttggtgtaca aggatttaaa attggctcag gagatttaac
       61 aaatttacct tttcttagaa aaatagatat gtatgggttg ccagttttat tatctacagg
      121 tatggctgat attcatgagg tcagtgaggc tgtagattgt ttcatagata gtccagttac
      181 tattttacat tgtacttcaa attatccggc tagttctgaa gatattaatt tattggcgat
      241 taacacatta aaagaaatgt tcgggatacc cgttgggtac tccgatcatt cattgggata
      301 tgatgttggt atttgtgctg ttgcgatggg tgctaaggta attgagaaac attttacctt
      361 ggataaggaa ctacctggtc cagatcataa agcttcttta gatcctcagg aatttgcaga
      421 ttttgtaaag catattagaa acactgaaat atttttaggt gatggtgtta agcgagcaat
      481 gccttctgaa cttagtacaa aagaagtatc aagaaaaagc ctagtggtca ctgagaattt
      541 aaatgtggga gatattctta cagagaaatc tcttacaata aaacgtcctg gaaatggaat
      601 agctcctaag tttttatatc aatatattgg caaaaaagtt aaaagaccaa taagtgcaga
      661 tagtgttctg cttgaggaag atgttgaatg aaaaaaatac ttatagtaag tggaacacga
      721 gctgattatg gaatatataa acctgttgct gagaatttac tacaagataa ttgggatgta
      781 tcatttgttg tttctggaat gcatttatcc cataaatatg gatttactaa agaattgatc
      841 ttagctgacg gatttagaat tagtggagaa gttcaaagtt tgtttttaga gaacaataag
      901 ggaaacatgg ctcggtctgt ggcattagag ataaatggtt tcacaaatat atttgaacaa
      961 actagccctg attttgtatt attactaggg gatagaggag aaatgttggc agctgcgata
     1021 gcttgtttgt atcttggaat caaaacagca catattcacg gaggcgaagt gtctggtact
     1081 gtagatgaat caataagaca tgccataacg aaaatagcat ctgttcattt tgctgctacg
     1141 gaaaaaagca aagagcgttt aataaaaatg ggggaagatt cttggagagt ttttcccgtc
     1201 ggagctccta gaatagatac tattttaaat aatagattac ctagttttag agatataaaa
     1261 gaaaaatatc atctaaaatt tgaaaagaaa aaatacgttt tgcaagtatt tcatccagtt
     1321 gtaaccgaac tagaaaatat agaaaaacaa attttagctt tgatcaattc aatggaacaa
     1381 caatctcaac caattatttg cattttacct aatagtgatg ctggtagcga aattatcaga
     1441 aaaatgtacc agaaatattt aaaaacggat attatttata ttacgaattt agagccggag
     1501 gaatacctga ctatattaaa agagtgcaaa tttatgatcg gaaattcttc ttctgggata
     1561 attgaagctg catcattcaa aataccagta ataaatttag gaaccagaca aaatggacga
     1621 gaacaatcta aaaatattat aaatgcaaat ctaaatgaaa atgaaattga aactgctttg
     1681 aataaaatac aagatcctca gtttataaaa agcttgaaaa atgttaagaa catctacggt
     1741 gacggcaaca gttctagaag aataacaaaa acattgaatc aaatattgaa tgatggggaa
     1801 gatattaagt ggattcaaaa acaaatagct tattaaaata tactagtgat ccatacatta
     1861 ttattggggc tggcggacac gcaaaagtca ttattgaaac cttaagatta gaaaataagt
     1921 taatatatgg attttttgat gataagcaaa ataaaatgca agatctgaaa tatcttggga
     1981 aaataaacga attgaatagc tgggcggata gcaaaaaaaa aatctataaa tttattgtag
     2041 ctattggtga ctgtgtagta agaaaaaaga ttataaaaaa attgaatatg ccaagtgagc
     2101 aatatggaat agctgttcat ccaagtgcta tagtggcaaa agaaatttca attggttatg
     2161 gaactgttat ttttgcgaat gctgtaatta acccagatgc tgtaattgga gagcatgcta
     2221 taataaacac aggtagcatt gttgagcatg attgtagaat taataactat gttcatttat
     2281 ctccaggtgt atgcttagcg ggtggagtac atgtaggtgt aggaactcaa ataggaattg
     2341 gttctcaatg tatccagatg aagaaaattg gatcttggtg tatgataggc gcagggagca
     2401 ctattgttaa ggatattccc tcacattcct tagcttatgg caatccagct aaaattaaga
     2461 aagaaggaat taattttgaa ttctagaatt ttattatcaa gtcctcatat gggaggaaca
     2521 gaagaaaaat atgttgctaa ggcttttaaa gataattgga tagcgccatt aggtgaaaat
     2581 gtggataaat ttgaagaaag tattacaaaa tatactggtt ctaagggcgg agcttctgcg
     2641 ttaagttcag gtacagctgc catacatctt gccttaagac tgcttaatgt tggaaaaaac
     2701 gatattgtct ttgctccttc atttactttt atagctacag taaatcctat tttatatcaa
     2761 ggggctgtac cagtgttcat tgactcagaa cctaatacat ggaatatgag ccctgttgca
     2821 ctagagagcg cattagaaga agctgaaaga aacaaccaat tacctaaggc aatcatcgta
     2881 gtgaatttat atggtcaaat ggctgattat gaacgaattt tagctattgc aaaccaattt
     2941 aatgttccag tgatagaaga tgcagcggaa tcgttaggtt ctacttataa gggaatttcg
     3001 tcaggcttat ttgggacaat tgggatctat tcatttaacg gtaataagat catcacaacc
     3061 tcaggtggag gagctctagt gtctagtgat aaaaaatatg ttgagcattc aagattttta
     3121 gcaacacaag caagagatca agcaccgtat tatcaacata gcgaaatagg ttataattac
     3181 cgtctaagca atatttctgc cggtatcgga cgtggacaaa tggaagtatt gaatgataga
     3241 gtagctaaac gaagagaaat tttcaatcga tataaggaag ctttttctgg aattgataga
     3301 attgaaatga tgccagaagt ggatggcatg gtgccaaatc gttggttgtc tactttaacg
     3361 atcaatgatt ataattctat tgatcaagta gttaacgtaa taaataaact aaatgaaaaa
     3421 aacattgaaa caagagcttt gtggaaacca ctacatttgc aaccagtatt tcaaggggta
     3481 aaaatgtatt accatgataa taatcttagt gtgtcagaaa agttatttaa tagcggctta
     3541 tgcttacctt ctggaagtaa tatgacagat agtgaacaag aatttgttat tgatgagttt
     3601 aaaaaaatat tcaagtaggt gtgatatttt atgaacatag gaattattgg tttaggttcc
     3661 atggggaaaa gaagacttag actcattcaa gaacacttta atgatatttt actatgtggt
     3721 gttgatacta atgaggcgag acgaaaggaa gttgaaaagg aatttggaat accaacttat
     3781 aatagtctaa cagaggcaaa aaatttgttt gaaataaatt cagtttttgt ttgtacatcc
     3841 cctataaccc atgaatttat tataaaagag gctttaaatt tagattgtaa tatctttacg
     3901 gaaatcaatt tattaaatga atattatgat gaaataatcg aagtagc
//
LOCUS       DAZBSA010000159         3878 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000159 DAZBSA010000000
VERSION     DAZBSA010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.163"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(718..3867)
                     /locus_tag="ACHGMT_002714"
     CDS             complement(718..3867)
                     /locus_tag="ACHGMT_002714"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFN6836407.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         1076 a          803 c          637 g         1362 t
ORIGIN      
        1 ggactaatac aggtagtcct cttttttgtt attttgatat tctaaatcat tttttattgg
       61 attattccct tgttcgatat agacccaaat tgatctatca ataattatca aactgagtca
      121 tatagagaca aaaatttcaa aaatgacctg ttaaatggtc atcgaatatc atttaaaatc
      181 aatacgacgg agtcagacac ggagtcaaaa caagcccgct gagagtaaaa aatatataca
      241 gtcccatagg taaagtgcca caggactgta tattttttta tttgagatta cctgaaatga
      301 ttccatttac tttttatctc attttttcct actagttagt atcaaatact ctcctatttt
      361 ctcaataaac tattttgggt tacatggtat ctgaaacagc taaaaccttg tcattacagc
      421 aaaaaatcac tttatctccg tttcatttca gatacatttc acttaagcgg attccaaaaa
      481 aacaaaagaa aaaggaaccc ctagttagcc tatgcttttt attggttcct ctcaaaacac
      541 ggtcatataa caacggggtg gggtaaatga atgaaaaagg tggaggaaac attattcaat
      601 agctgtctta aatacttcgt tataaaatgt gaacaaataa aagacatcta tccctatttt
      661 ctacacttcc actttactac catctttgaa tcgtacaatt ttcttaactc ttctcgttta
      721 cctcaattca ttttcaagtg gaatcagctc atcatctaac attttacgcc atgctcgctt
      781 catttcttgt gggtatttaa atggcttcgc ttctggatgg ttggctttat atccttcgta
      841 atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg
      901 cagattagaa gcatgtaatt ctttctcaga aacaaaccac tcattggaaa agacatgaat
      961 caaatcatca ctcgcttgtg taaaatagcg acgtttaatg acaaagatat cttcattact
     1021 atctaaagtc cccacatcaa acgcatttaa aatttgtcgg taaacttctt tgactccttc
     1081 tgttttattc aatttagcca aagctttttc aatttcttct cgtgcatctg aactattcgc
     1141 cgcataagtt cctagtaatt gatcaatata agtagaatca atatcataca acttcgtggt
     1201 attttcacta taaaattcga tcgtggaaaa atcttctccc tctttttctg gttcttcctc
     1261 tattaatgaa cccttcacgg tctcgtacac gccaatttgt tcttccaaaa gttgaatttg
     1321 attgttcaag gttgaagatt tcaccatgtc atcattgtaa tcgtcataag tcactaacga
     1381 ttcataagca cgattcaatt cttggaatgc tttgacgtac tcgatacgga tttcaagtgg
     1441 cgtatgttca tcaatggatt ccatggatga aacctctttt aacttaccat atgcttgctt
     1501 gaatcttttt ttggattcct gataacttgg ataaatcaaa cccgattctt cttttgcttc
     1561 tgaatacagt tttgtcgcat cctcgacatt cttctccatt gtggctggct ttcggaaagt
     1621 aacaattagt ccttttgttt tttctggata cgtacggttc gtacgtgaaa aagcttggat
     1681 caatcctgca tactctagat tacgatccac aaatagggtt tgaatcgtag gtgcgtcaaa
     1741 acctgtcaat agacgatcca ctacgatcac taaatcaatt tgtttcccaa attgtttgaa
     1801 ttccgctctt tttcgtgcca aacgattatt gatgtctccg ttataacgct caatgtctgc
     1861 taatgaccag gaagtatcat aatactgatt atattcctca ataatttttt gcatttcttc
     1921 ttgttggaca cttgaatctc ggctgttttc ttctaatgaa taggtaatag cgattcgtgg
     1981 aaaatcagga tcctccatcg ttcttccttc acgaatgggc tgattaggaa actcttttgt
     2041 taaccagtca ggttctttcg tcatttcttt gatcgctcga taatactttt tggccatgtc
     2101 aatggaactc gtggttaaga tcgcagactt cgttggacgg ccatttcgaa aatcaaattt
     2161 tgtataggca ttgtctggac ggaacatttt atgaatgact ttttggatat gttcatctgc
     2221 ttcaaaaaca gtagggtcca aataactttc ttttttgaca ggctccattt gatcaatcat
     2281 acgattgatc tgttccggtg aatactctgc atatgtttct acttctttta atttccgata
     2341 gattttattc tctaatgaag tcggctccac ggtgttttca tgctctactt gaaatcctaa
     2401 aaccgagcca tcttctaatg cattcttgat ggtataggta tgcaagacct ctccatactg
     2461 atcatgggtc gtacgtgcca attgcccttt ggcttgtttc cgattctcag ggaaaatcgg
     2521 tgtacctgta aagccaaacc aagtggattt cggaaacatt tttttgattt cttccatatt
     2581 ttcagcactt aacgctcgat gacactcatc tactacaaac acgatatgtt gccccattaa
     2641 tttttggaag cgattggttc cttttttctc ttcttgtttt tttgcatatt tcaacgccgc
     2701 atctagcttt tgacgggttg taataatcac aacatttgca ttggcatctg ccaacagagt
     2761 ttcacttaac tctttggcac ttcctgttcc cacgatcaag ctattcgctt tggcgttccc
     2821 ggaagaaatc ccagtattaa attccgaagc aaatttcgta aactctgtgg tggtttgatt
     2881 atctaaatct ttgcggtcca ctaacatgat cgtccgatcg atccccgatt ttcgtgctaa
     2941 aagcttagta gatacaaaac tagtcaacgt ttttcctgaa ccagtcgcat gccacacata
     3001 tcctgattga tgtttatttg cagcaataaa caaggcttga atcgcatgta cttgataagg
     3061 atgtaacacc atgaggattt tattgtcttg atcttcactg acaatcgtat aatccgcaat
     3121 caagcgatga gcatccggga tatttagcac ttgtttgcaa aactcatata agttttctac
     3181 ctttttatta tctttcgttc gccaactaaa taaaaactta ggatgcaaat cctttggtaa
     3241 ggcatttgca aaataccgag tggtttgttc attggaaacc acaaacaact gtaaggtcga
     3301 aaagatattg ttccgaaaca taccttcttc tgcatatttt ttgatttggt taaacgcctg
     3361 ataaaaacta tcttttgcgg ttacttgttt cagctcgatt tggacaatcg gtaatccatt
     3421 gatcagtaag gtgacgtcaa aacgtcgatc ccgtgcttct atgttacttc cacgttttgc
     3481 aatttgatgg accacttcat aggtagagat tcctccacca atgtcttgat tagaataaag
     3541 aatgagcgag acagaaccca gttgcggatc ttcccgttca atcgtgatcc gagccattcc
     3601 attttcacct tttaaccatt tcgcagcatc aaagggggtt ttcgttcgta gtaacaattc
     3661 tgttttgatc gtttcaaatt ctttatcggt caaaggaaaa tcattcagtt ctgcttgatt
     3721 atttgagatg attttttggc gtaaattgac ccaaagatct tcttctgatt ttagatcggg
     3781 acgatacgtc cactgattat gcccttcccc caatacacgg atcaactgtt cttcgacttc
     3841 cgcttcctca cggtgactaa ttttcattta tcttccct
//
LOCUS       DAZBSA010000160         3807 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000160 DAZBSA010000000
VERSION     DAZBSA010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.164"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(142..468)
                     /locus_tag="ACHGMT_002715"
     CDS             complement(142..468)
                     /locus_tag="ACHGMT_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836408.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(505..870)
                     /locus_tag="ACHGMT_002716"
     CDS             complement(505..870)
                     /locus_tag="ACHGMT_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFN6836409.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(907..1122)
                     /locus_tag="ACHGMT_002717"
     CDS             complement(907..1122)
                     /locus_tag="ACHGMT_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN6836410.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            1251..1586
                     /locus_tag="ACHGMT_002718"
     CDS             1251..1586
                     /locus_tag="ACHGMT_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN6836411.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(1662..3659)
                     /locus_tag="ACHGMT_002719"
     CDS             complement(1662..3659)
                     /locus_tag="ACHGMT_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836412.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
BASE COUNT         1191 a          756 c          490 g         1370 t
ORIGIN      
        1 acactgctgt tataacagtg actaatgtaa agattttccc agcaatcatc agactattat
       61 ttacagggaa tcaaaaaaac tccttagtaa gcttacacct ttaggagttc ctgtttgaac
      121 ggtatgcata atacatttaa atcaatttaa cattaacacc tcagtatatg gtatctctac
      181 atatacacgg tctttttttg gatagtactc gacatttata ttgatagcac ttccattgtc
      241 taacgtagct ttagcccggt acgtgttatc accattatca tgagtgatcg tcacatcatc
      301 acatgtccga tttaagttat actgttcttg taaaatctga gttaccaatg gtacagatga
      361 cttctctagt ccagatgaac ccgtcattct tgaaaaaatt atacctataa tagccaatac
      421 aacgatacct ataatgacaa acttcttatt catatttact ttattcaact ttttatctcc
      481 tatctccaaa atgattttct ctcatcagta actcattgac atccccaata gtacaccaaa
      541 gatggctcca gctacccctg ctatcatcaa aatcgtaccc atttgttcat ctttctctcg
      601 atataagtaa cccataataa cagcaacagc accaaatacc acaggaataa atgctaatga
      661 tactaccata gaaatccaac caataatcaa atacagcttc cctgtctctt gtccatgtac
      721 gatttgttcc ttcatttcat taattgtctg ctcagtactt ttctttactg tgtctgttcc
      781 ggttgtctca tctgtttctt gtaatgctcc gcaaaactgg caatacttac tttcatttgt
      841 tatttcttta ccacattttc tacaatacat gtcttctcct cctaaaatca ttcaattgct
      901 tattagctaa ctgtttactt tattaaatac atcagaaaac agctcattta ttggtacact
      961 taatatctca gaaagcattt tcatttgata gggtttagga atcgttctcc ctgtttgcca
     1021 cgaacttaca cattgctgtg taacattcat ttttttggct aactcagttt gggaaagtcc
     1081 tttttctttt ctgtatcggc tgagattact tacttttttc atgattctcc cctcctgttt
     1141 tattatatac aacaatatga tgtatttcaa ctcataaaat atataaatac ttcaaatgtc
     1201 attacaaata caacaacttg ttgtactcta ctgagaaggg gggtgtatta atgaaacaga
     1261 agcaatatgc aaaggtcatt agagaaaagc gaaaagcact cgggtggaca caaaaagaat
     1321 tagctaaaaa aatcttctct acacaacagg cagtagcaag atgggaaaat tctgtaacag
     1381 aacctaacct cgatagctta acagctcttt ctagagccct aggaacccct gtaagccatt
     1441 ttctagacaa tgttgacata gattatgagg aagagttttt ggctctctat cgctctctta
     1501 gtactgaaga tgcaattcga acaattgact atatgaagct attaaaaagg caagaaaatg
     1561 aacgcaatca gctattgaaa gattgaaaaa taaataaccc accgaacttt aacggtgggt
     1621 gtattgagat gaaataagat taagagctat ggataacctg tctagtttaa tataattggg
     1681 tctcttctat tccttactct ccgtccatca tctcttgtta cacttgtaaa ttgataaata
     1741 ggaactaatt caactcctga taatatcaat tcttttatta cttcatctat ccctgcccct
     1801 ttcgctagat atactaaaaa actactgctt gctttcacat tgtatagttt cttaatatcc
     1861 ttagaagctc gttcaagagt gaatccttgt ttaacaaagt agcctctgtt ttctattaaa
     1921 gcatttttca cgctgttatc cctgacattt cgtaggcgtt cacaaacagt gttaataagt
     1981 tctttttctg tcattagata tgtcccttgc cacgataagc cctgtaaaac ttcatgtgct
     2041 tcatctaaaa tcaattgttt ttccttgcta agttctgatc gggaattttg aggaaatact
     2101 cttttagcct ctttttcgtt aaaacatatc ttaacccatt cataagaaaa atattgatat
     2161 gtaaaatgaa atttttccag aagtggaagg atatcttcat tcaatttttg aatcacagaa
     2221 gttgcattaa aaacgtctaa tcgatagaca ttgcttctct tatgtttaaa gtttcctaac
     2281 gctttctctt cctttaacct atttaggtat tcgagcatct tcaaaggaac atcttcatca
     2341 tctaattttt ttatcaatcc tataaaagtc ataagtgata gtaacttatt acatttatct
     2401 atgtcataag ttttaccata gtaagaactc atttttttag ctaattgttc ccccgacaaa
     2461 atacaagtca cactttcctt actatcatca aaaaaattaa catcagaaag caagacatca
     2521 tagacacgtc gcatttcctc tagataattc cttattgtcc tgaaaagttt gtctttcatt
     2581 gattcaagag tgtcaaaaag tacttttgat tcctctttca attttcgaaa atgtgtagga
     2641 tacaagtcta ggttctctaa tagccaatta aatgtttcta cccgactgtc tttctttaaa
     2701 attagcatca caactccaat aaaattttca ctgatatcac aattactgga atgacaataa
     2761 aaaatggaat gcccagtttt actggtaaaa atactagcag agggattatg atcgttgtga
     2821 aacagacatc ttaagttttt acttgactta agtaatttat tcatatcaat ggttaacaat
     2881 ctttcataaa tctcattaaa agacatgtcg gaggaatcaa acaggcagtc tttaaaccac
     2941 tgtttcattt cttctttgtc gtcatttttt atcaacttaa caaaatcggt ttcattgact
     3001 agttcatact ctctccctga atcactttga caatttttag aacatgtcat ggtaaaatca
     3061 atcggaagct cagtaatatc taacatgttg tcttcattaa ttattatcac tgcatctgac
     3121 ttatatcctc caaaaaacat tctattcgaa gcatttacat tgtcatcaca cccggggatg
     3181 ttctttttca agtattcgta aacagcaacc attttaccat agtcagtaat tgcttctttt
     3241 aatagaaaga caatcttgta tttttctaga ttttcagagt accgaatact tttttgtatc
     3301 gcaaacgcag aattatgaac taaattgata gataaaacct tttctatgct taagtatcta
     3361 ctatcatctt tactcaaatc tactaccttc cccttctcat ctttaacagt gttatcgaca
     3421 tccagaataa ttactctggt ttccacaaaa ctctccttgt cattgccttt attctttgtc
     3481 ttgcttggaa taaatgattg tgatgaattt agtatctcta caaactcatc tcttgtaact
     3541 tctctatagt tatctaaaca cctatctctt acatcagttt tttctttttt tgtaactggc
     3601 ttctctaata atttttcttt atctacataa attggaatat taatcattga tgtttccatt
     3661 tgtgtttcct ccttataaaa ttctgttaaa gactaaaaca tacagataat aaaaacaaac
     3721 tctttattac tatatgtttt agtcttattt gtttactcaa cctttatacc gttccatgaa
     3781 actatcaata tcagattttt taattct
//
LOCUS       DAZBSA010000161         3717 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000161 DAZBSA010000000
VERSION     DAZBSA010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.166"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..155
                     /locus_tag="ACHGMT_002720"
     CDS             72..155
                     /locus_tag="ACHGMT_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFN6836413.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            280..1017
                     /gene="erm(B)"
                     /locus_tag="ACHGMT_002721"
     CDS             280..1017
                     /gene="erm(B)"
                     /locus_tag="ACHGMT_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFN6836414.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1280..2143)
                     /locus_tag="ACHGMT_002722"
     CDS             complement(1280..2143)
                     /locus_tag="ACHGMT_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFN6836415.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2145..2417)
                     /locus_tag="ACHGMT_002723"
     CDS             complement(2145..2417)
                     /locus_tag="ACHGMT_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFN6836416.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2434..2643)
                     /locus_tag="ACHGMT_002724"
     CDS             complement(2434..2643)
                     /locus_tag="ACHGMT_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFN6836417.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2721..3617)
                     /locus_tag="ACHGMT_002725"
     CDS             complement(2721..3617)
                     /locus_tag="ACHGMT_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN6836418.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
BASE COUNT         1143 a          652 c          575 g         1347 t
ORIGIN      
        1 aaattttgta taataggaat tgaagttaaa ttagatgcta aaaatttgta attaagaagg
       61 agggattcgt catgttggta ttccaaatgc gtaatgtaga taaaacatct accgttttga
      121 aacagactaa aaacagtgat tacgcagata aataaatacg ttagattaat tcctaccagt
      181 gactaatctt atgacttttt aaacagataa ctaaaattac aaacaaatcg tttaacttct
      241 gtatttattt atagatgtaa tcacttcagg agtgattaca tgaacaaaaa tataaaatat
      301 tctcaaaact ttttaacgag tgaaaaagta ctcaaccaaa taataaaaca attgaattta
      361 aaagaaaccg ataccgttta cgaaattgga acaggtaaag ggcatttaac gacgaaactg
      421 gctaaaataa gtaaacaggt aacgtctatt gaattagaca gtcatctatt caacttatcg
      481 tcagaaaaat taaaactgaa cactcgtgtc actttaattc accaagatat tctacagttt
      541 caattcccta acaaacagag gtataaaatt gttgggaata ttccttacca tttaagcaca
      601 caaattatta aaaaagtggt ttttgaaagc catgcgtctg acatctatct gattgttgaa
      661 gaaggattct acaagcgtac cttggatatt caccgaacac tagggttgct cttgcacact
      721 caagtctcga ttcagcaatt gcttaagctg ccagcggaat gctttcatcc taaaccaaaa
      781 gtaaacagtg tcttaataaa acttacccgc cataccacag atgttccaga taaatattgg
      841 aagctatata cgtactttgt ttcaaaatgg gtcaatcgag aatatcgtca actgtttact
      901 aaaaatcagt ttcatcaagc aatgaaacac gccaaagtaa acaatttaag taccgttact
      961 tatgagcaag tattgtctat ttttaatagt tatctattat ttaacgggag gaaataattc
     1021 tatgagtcgc ttttgtaaat ttggaaagtt acacgttact aaagggaatg tagataaatt
     1081 attaggtata gggcacctct aataactacc aattaattca cctcaaaatg aaattagaaa
     1141 aaaacagcga aatttttaaa tctatttctt atcgatacaa attcctcgta ggcgctcggg
     1201 accccaacat gaggaaatgt ttttataaaa aaagagtagt taccgttttt tggtaactac
     1261 tcttttcctt ttttaaggtt taaatacctg gaagtttagg tgttttgggt attggtggtg
     1321 taggtagctg caagctttcc attttttgtt gaattgcttt aaattcaggg gtttcttggt
     1381 gttgattttg aaccattttt tgctctattc tctccaaagt cggttgaatt tctttccctg
     1441 atactttacg atttaattct ctttctaagg tctctttcgg actaatggaa ggcgtttcta
     1501 agcttgaata gagtttcact ccttctctat tgtaaagcct tatatcgcta aataagcccg
     1561 ttttatgaag ggtttctaaa ttggtcggta agtttttgac aacaatatca tgcgcttgtt
     1621 ttggtgttgc cctagctgtc attggatcat ctgcatacat ggtttcatat cgttcaatcg
     1681 ttcctaagta tgattcaatt ttcggtactg ccatgacgta tatttttgtt tcataacctt
     1741 tagattgaag cattgtggct gtttgaatgg gaacgtctgt tgttcgtcct gttccttcaa
     1801 tcactaaatt atagccttga tcgcttaaac ggctaataag tgcttctgtc atgcgattcg
     1861 aataaggggt aacgtgtttt actacgtctt tttcatagag tttcactaat tcgtcaaaat
     1921 ttgggtgctg ttgtttaaag gtatcattat caatgacaat aacattccct tgtgtttctt
     1981 caaaaattgc tgatcgcaaa ctggttttcc ctgaccctgg ttgcccacca agtaaaaaag
     2041 cggttggcga ttcaaccgct agtctatttt tagttagtcg ttcaacattc ttttctaaac
     2101 gatcttcaaa ttgcttttcg gtgaagtcag taatattcgc catgttaagc cactttctct
     2161 ttattcaaaa tttgttttga atattgattc attgaccgcc aatactcatg aatagcttgt
     2221 aattcttcaa atggttgttg aaataaatca acttcttgtg ccacttctaa ttgatttaaa
     2281 agattcactt ctagtaaacg agtattttta gtatctagtt catgattcaa aacataattt
     2341 aatacccgat tataaacact tgccgataac tcattaatga tttcaatttc aaatgttttt
     2401 tcatacgtaa ctgccatttc attttcactc cttttaaagt ttgtcaggta aatatttccg
     2461 tatatattct tctaaggctt gatccataac ttcttttacg tttccgccat tctttgctgt
     2521 ttcgattttt atgatatggt gcaagtcggc acgaacacga accgtcttat cccccattat
     2581 atcttttttt gcgctgattg gcgtatcatt tcgttttgct ttctgtgcgc ctaaatttcc
     2641 cacgatcgct cacttctttc tatttcttct tactatttat tttatcatca cctatcacaa
     2701 atcacaagtg atttgtgatt ctattctttt tcattttcta attgaatgat tcgctcaatc
     2761 atttcaaaaa atacgttctc atacatagac aaaacttttt tgtcatagcc tttgtgttct
     2821 gtaatgccat ttttagacca agtacttact ttattacttc gcttgataat atttcggaaa
     2881 accaaattat cctctttatg ttctttgtac agttcttcca ggtttgattt tatcgttgcg
     2941 ctgtccgtat caactaaata aggaacaaaa ccaatcatat ctagtccagg gttaaactgt
     3001 tcttgcaaat caatcaaata ggaaatatag ttttgaatgt tgtttgtact ttcttcttct
     3061 gcttgtaaag ggatcataac gtaatcactc gccacgattg cattatttgt ataaacgctt
     3121 ggcgttggta cagtatcaat aataataaga tcatagtcac tttttaaagg tgctaaaaga
     3181 gtagcaagca atctactttc attttcaaac gtccatgagc gagttaattt tggcagtaac
     3241 atcaaatcaa acgtgccagg gatcaagtct aaattatcag tcaaatgaat aatagaagaa
     3301 gccaagtttc catttttcaa gccttcataa aaattgacac gtggcaattc tacctcaaaa
     3361 gtttttgcta agtcttttgt caatgttgcc tgtaagtcct tatcgatcat taaaactttt
     3421 aaattcaatt tgtctgtcaa gtaagcaaac atagtcgata atttggactt tccaacacca
     3481 cctttaaagt aattattcaa gataataatt gcttcatttt tattgtttaa aatacgtctt
     3541 aattcttcaa gtatttttag tttttccttc tccataacta cgccccattc tttttttgta
     3601 tagtaatatt gtatcattaa gaatcacaaa tcacaagtga tttgtgatta atcacttgtg
     3661 atttgtgata agtgatttgt gattaatata taaaagccct ctttaaaggg cttttat
//
LOCUS       DAZBSA010000162         3706 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000162 DAZBSA010000000
VERSION     DAZBSA010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.167"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..353)
                     /locus_tag="ACHGMT_002726"
     CDS             complement(<1..353)
                     /locus_tag="ACHGMT_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836419.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRINESDYNKRDQVLL
                     VKLNEEISVEEAVRT"
     gene            complement(613..1161)
                     /locus_tag="ACHGMT_002727"
     CDS             complement(613..1161)
                     /locus_tag="ACHGMT_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836420.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1162..2019)
                     /locus_tag="ACHGMT_002728"
     CDS             complement(1162..2019)
                     /locus_tag="ACHGMT_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFN6836421.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2305..2591)
                     /locus_tag="ACHGMT_002729"
                     /pseudo
     CDS             complement(2305..2591)
                     /locus_tag="ACHGMT_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2581..2910)
                     /locus_tag="ACHGMT_002730"
     CDS             complement(2581..2910)
                     /locus_tag="ACHGMT_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFN6836422.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2987..3259)
                     /locus_tag="ACHGMT_002731"
     CDS             complement(2987..3259)
                     /locus_tag="ACHGMT_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFN6836423.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3259..3522)
                     /locus_tag="ACHGMT_002732"
     CDS             complement(3259..3522)
                     /locus_tag="ACHGMT_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFN6836424.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
BASE COUNT         1083 a          752 c          472 g         1399 t
ORIGIN      
        1 aatgttctta ctgcctcttc cacactaatt tcttcgttca atttcactaa taatacttga
       61 tctcgcttat tataatcgct ttcatttatc ctaagagtca aatctttttc caccataact
      121 tttacaccca ataaattttg cggtttattt ttctctgttg aaattctttg tgtagccttt
      181 aaactgacaa ttagctcatt gggtacatac tctatttgag agtcatctaa ttggctcata
      241 atcctttctt tttggttagt atctgcagtt acttgtttag tatgagccat gaacccaaac
      301 atactcaccc caacaaccaa tcctaaatat attttctttt taaacttttt catctacatc
      361 tcctcctaat agttttattt tagagttagc tctattactt atttactaac tcaacaacag
      421 attatcatac acttgttaat gaaatcacaa tatctcatta aaacacaccc taaaatcata
      481 accgttccta tgctcatttt ttggattttt taatcacgta accagaaaaa taaatcccct
      541 ctatttcacg caaatcaaag aagaactaag taactaccgt ttaaaggtag ttacttagtt
      601 cttctttgat tattatctaa ctttattatt cattccccac cctttgcgca gtgagtcttc
      661 ggtatcttgt tttatctgac tggtagttct tattatttct ccacgatcat ctaattctgg
      721 aacaattgga tatttttctc tgaacaagtc tagttctttc accacgtcat taaacaaatc
      781 ttgcttatct tcttgcgtaa gattctgttt aatatactca tctaaagctt ttctaagcaa
      841 ttcatctctt gtcatattag tctcatactt ttctactaaa actctggaag tcgcatttaa
      901 caaagtcgac agttttaaag gatgcttggt ggaataaacc ttatcaaact ttctgattgg
      961 tgcgccaact ttttttgttc ttcctctact ctgtctttca acattggagg aagtttttat
     1021 ttcttgaact tggtgcactt ctaattgctt attttcagat ggtgtaattg cttgctgctt
     1081 tttctttgct tctgcttctt tttttatttt ggcgagtcct ttgttgttcc caaaaatatc
     1141 aagtcctttg atgtcagcca attactccac ctccgcatac caattctcgt gttcttccaa
     1201 ttctttcaaa atactgataa ataattcgtg gcttttgcga tcccattgtt ttaagagacc
     1261 tttctttgtc ttctccatcg taatacctga tccgtcgtat cgttttaagc gcgcattttt
     1321 gagtacaaca tttttaatca aatgttcccc ataaatctct tgggcttgtt tatacatttc
     1381 ctgatctact gcgtcaccgc tttccaacat gaaaggaata acaccagcaa catataaatt
     1441 tgtatcaaat tctttcacaa aaaagtccat atactcaaga tattgcatcg catttctcat
     1501 cgattttact tgggtctgta agacgacgat tgcccaatcc gcaatgtaca tcgcggattt
     1561 actatataca gaaatagaag gtgggacatc taaatagatg acgtcaaact catcaaataa
     1621 tggttctagc ttttctttca tatatttgaa ttgggataat gaatcattat attggtcttt
     1681 gatccaatca tcatacattt caaaggacat gtctgctgga agtatataga ggttattgat
     1741 tactttatag acagcctctt taggtggaag attgtttatc aacacgtcag tcacagtatt
     1801 ttcgtaaaca atcggttcac cttctccgcc tccttcttcg ttagtgaagt tcatatgcgt
     1861 taagttcatc acatcagaag tatttccttg catatccaag tcaattatca aggttctttt
     1921 tccttcatta actgctgatt caaaccctaa catttgcgcg cacgtacttt tacctacgcc
     1981 acctttaaaa ttaccgatta catatttctt cgctaccacg ttcctacctc ttttctttta
     2041 gcaatattca tttgatacta ttataacata tttattttta gttactacca aaaaaaagat
     2101 atatatgtac taccttataa ttaataagta aaaggtagta cataaataag gaaaagctga
     2161 aatatattga tttatagctt tcctcaaagt aatgacgtta tttaatttat cgagaagcag
     2221 agaatactcg cgactttagt catgtagacg aatcagattt tcgataaaaa agaagccgtc
     2281 cttcttaaga acgacaacaa atcactagat taattttttc aactgttcat aaaagttttc
     2341 atgcgttcca cataacaata ctggaataac cttgttttct ataatctcgt aagctactct
     2401 atatgttgtg cccgcatatt tgaacccact agaccaaata cctgataaat ccccagtttt
     2461 ttgttcaccc ttatatggat caatcgcaat ttcatcataa attaaattca aaaatttttc
     2521 tttcaatggc ttctctttta gctttttaaa tacttaatga catgttttga aggaacgatt
     2581 tcataagcca atttctgttg ccaactcctc tttagtcatt actttagaat tagtaagtgt
     2641 atcttcagca atatctctaa aggaagaatg cattttgttt tttctattct taaattctac
     2701 aagaatctca tctttatcgt agccttctgc aattatatca gacaaaatat cttcgctaaa
     2761 atcataaaat tctttttgtg gttcaacaaa ttcataaaga ataccattct ctaccaactt
     2821 tgcttcaact tcgactccat taggaacatt aaaatcttta ggtactgtca acatgattga
     2881 attgccttgt tttcttgttt ttgtaatcat ttaaatccct catttctaac agtatgtaat
     2941 tacttagtaa atacattata ccacgagata atgtatttac aagtaattac tgtgactcgt
     3001 aatgcgtcct tgctgaataa atttcaacca cgtgttcatc ttcatctacc ttataaacaa
     3061 cccggtgttg tctattcaat cttctagaat acctaccttc atgtgtaggt tttaacttct
     3121 caaatgattg ggtagggaga tagggatctt cctttaaagt ctgaataacc ttctcaaatt
     3181 gaggtttcaa attagactgt ttgattttct ttaaatcagc tttagccgag tttttaatca
     3241 tcacagaata gttattcatt ataagttatc ccaatcaatt tcatcaacat tagtaaatcc
     3301 actatcatct ttttctcgct tacgaacaac acccaaagtg cctatttgct caagtaatag
     3361 cgtttctttg attgctttcc aatcatccaa accaataagc accgcattat tttcactgcg
     3421 ctcactaatg atttcaattt ccgtatgatt ctcatttaca tctttcaaca acttataaaa
     3481 atttttccgt gctgagctag gatttaagac tgtttttgcc atatctactc cccctctaat
     3541 ttatatctcc attctaacgt acttaattaa gtacgtaaat tcgattgttt tgtttaaatt
     3601 ggagtaccaa aacagcattg taaagtgtta tccgtttcct ttatacccgt ggtgctagtt
     3661 aattttttcc tagaggaaaa tgtctaaaca tgttggtaaa tcaaca
//
LOCUS       DAZBSA010000163         3693 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000163 DAZBSA010000000
VERSION     DAZBSA010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.168"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..492)
                     /gene="greA"
                     /locus_tag="ACHGMT_002733"
     CDS             complement(13..492)
                     /gene="greA"
                     /locus_tag="ACHGMT_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFN6836425.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(628..1803)
                     /gene="mltG"
                     /locus_tag="ACHGMT_002734"
     CDS             complement(628..1803)
                     /gene="mltG"
                     /locus_tag="ACHGMT_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFN6836426.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            2007..3176
                     /locus_tag="ACHGMT_002735"
     CDS             2007..3176
                     /locus_tag="ACHGMT_002735"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFN6836427.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(3443..>3693)
                     /locus_tag="ACHGMT_002736"
     CDS             complement(3443..>3693)
                     /locus_tag="ACHGMT_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836428.1"
                     /translation="GKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKV
                     TIRYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT         1160 a          696 c          574 g         1263 t
ORIGIN      
        1 ctttcagtca gactacgctt gttctacttt gacgatttta actaacatat cgccacctgg
       61 agtagcaata gttacttcat catttttacg tttgccaatc aatgcttgcg caataggaga
      121 atcattggaa attttgccag aaaacggatc tgcttcagcg cttccgacaa tcgtgtactc
      181 ttcttcttct ccatctggta gttcgatgaa tgtaactgtt ttaccaattg atacctcatc
      241 tgcatctaca ccgtcgttat cgatgatttg agcaaaacga atcatatttt caattgttgt
      301 gattcttcct tcaacaaatg cttgttcatc tttcgctgat tcatattcag agttttcgga
      361 aagatctccg aaaccgcggg caattttgat tcgttcaatg atttctccgc gtttgactgt
      421 ttttagttct tctaattctt tttctaattt ttctttacct tctagagtca ttggataaac
      481 tttctctacc atgtgtaacg tctccttttt tcgataaaat accgcaaaag gtaatctttt
      541 gaggaatata gtccaaaaaa gattaccacg cttttcttca aatgtaaatg tttttctaaa
      601 aatgatcaat gtctagagcc tgtttttcta agaattattt acatattttt ccactaattc
      661 catatgttct tcataagttt tagcgaaata aacatttcca gtgctcgtat ctgctacgaa
      721 ataataatag tcattttctg ctggttctaa tactgccttg attgcctctt cgcttggatt
      781 gtcaaaaggt ccaggtccat agccggtatt ggtatacaaa ttatatggtg aatctacagc
      841 tgtatcttca tacgtcacta attctttgtg ttcacctaat gcgtataaaa cagagatatc
      901 tgactgaagt ggcatatttg ctttgattcg gttaaagaag acttgtgcaa tgtttttacg
      961 atcattttct tttacgcctt ctttttcaac tagagaagct agagttaaaa cttcttgtac
     1021 agtcaaattt ttctgcttga tagctgaata atagttttgc atgaccgtat tcgtcttgtt
     1081 caccatttca atcactagat ccttaagcga agtactcgtg taataatcat atgtggctgg
     1141 gaataagtaa ccttccaaac ggtattttgt gttctcagct ttagatgcac tttccaacag
     1201 ttctgggaat ttttggtgca actcgttaaa gaaagtctca tcattcacta aatctaaaaa
     1261 ttctttcttg tctttaccag ttaccttcgc tacccgttca gcaatctgat cgatatcgta
     1321 cccttctgga atggcaattt tcccatctgc aacttttgtt ggctcagaag taccgccttc
     1381 ttgcaactgt ttgccaatct cgtcaagagt catattaggc gctaactgat aatagccagc
     1441 ttgaaagcca gttaaatttt taaattttgt ataataattg aaaacaatcc cgcttttaat
     1501 aatattgtcc ttctccaaaa tctcaccaat ctgcttgtta gaagagccac tcgggatctc
     1561 gacttggact aattggtcat ctgatttatc taaaggcttc aacccgctat ctacataacg
     1621 atacacggta aatccaagaa atccgccaat aatcaataat gtaagagcaa tcactaaaat
     1681 gatcttcctc acaatcttat cttctttttt acgcagattt ctccgcttgt cgattatatc
     1741 tgctgtagct gccttactgg cttctttttt atctttactt gggtgttgtt tgtttcctga
     1801 caaaacaact cctccttact atcaagttcg taactttatt cattatacat gatatttgaa
     1861 ttggcgtgaa tgattttatt taaatattct atcaaaaatg taagtagtaa atccattact
     1921 ataagattaa aataaatgtt atcaagatac aagctatttt agctttattc tttctgtaaa
     1981 aaagaaagga gactaaataa actattatga aaaaaggaaa acatgctaaa aaaagaaaat
     2041 attcattttt aatgatcgtt gtcgtgttgt ttagccttag tgcgttctct acaggaacta
     2101 ttcttggatg gcaattttac aataatgaac actctcagat agttgcccat gctcaatcag
     2161 ctcaaaaaaa gttgatactg actaccaatg aacaacagct cgttgaaaaa atcgatcaac
     2221 tattaagaaa aaatgattat attggctcaa tttacgtaaa gaaaaataat cgcatcatca
     2281 tggaaaaagg ctatggctat gcgaataaac tcacgcaaca accaaatgat ccttctcttt
     2341 attatcagat tggttcggtt caaaaagcat tgactactct tttgatttta aaacaagtcg
     2401 agcgtgggca cctttcttta gatactaagc tggctcaatt ctatccacaa attgctggca
     2461 gccagcaaat tactatccgt gacttgctgt atatgcgctc aggcttgcac cgaactgctt
     2521 ctccaaccat accgatgact gatgaagaag tcgttcaatt tgcgattcag cacttaaaat
     2581 ttacagatta tcagacctct cattatgatt cattgaactt taccatattg actggaattt
     2641 tgatcaaatt aacaaatcaa ccgtatgaaa aattattgac cgatgatatc atcgaacctt
     2701 taggattaca gcatacaaaa ttttatgacc aagtcaaaaa ttcatccaat catgcactat
     2761 cctatcaaat gtctaaagag gatgattact ttaaaccact gacagaatct gaaacagaga
     2821 tccataacga attagggact ggtaatatca gtatgtccgt ctatgattta aatacgctgt
     2881 ttacacaagt tttatctgga aaactagttt ccaaagactt actcttctca ttatggcgtg
     2941 aaaatgcgaa tggacatcca tatagtggtg gggtatatag cggagataat tatatcctgt
     3001 cgcaaggaaa tatcgactct tttgatacat ctgctatatt tagcaaagat tcacaggatg
     3061 ctatcatcat ggaaagcaat ataaaagctg acaaaactat caaactgcct actgctgatc
     3121 taagaaacca aatctatgac ctgatggaag gaactactaa gttggcaaat agctgatatg
     3181 atgaaaagat agcaaatact acttctttga atagcatttg cttaaaaaaa gtactcgact
     3241 aaacgtagga aagagtgtgg gacaaatcta aaaatcaatt tttgctacag actttaattc
     3301 caaataaaca gaacagaaag aggtgttctt ctttctgttc tgtccaaatt attctaagat
     3361 ttctctttac caacattatt tgacaaaggg cctattaaat ctaaaatagt atctttagat
     3421 tgctaaaata tgtgctagca aatcattcat ggcttgttct tatttttaat tttatgcaat
     3481 aaaaaagaag aacaattagc gtgattatag aaataaataa tgagatttta gaaaaaaatg
     3541 tgtatgaata cctgattgtt actttattta ttccactttc actttttact gtaacaaaag
     3601 gacctccttg ttttagtact gtagcatttt ttgaattaac agtaaccgtt aatcctttat
     3661 accggaatac aggtaaaact attgttttgc ctt
//
LOCUS       DAZBSA010000164         3643 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000164 DAZBSA010000000
VERSION     DAZBSA010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.169"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(25..246)
                     /locus_tag="ACHGMT_002737"
     CDS             complement(25..246)
                     /locus_tag="ACHGMT_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836429.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(344..589)
                     /locus_tag="ACHGMT_002738"
     CDS             complement(344..589)
                     /locus_tag="ACHGMT_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836430.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            829..1443
                     /locus_tag="ACHGMT_002739"
                     /pseudo
     CDS             829..1443
                     /locus_tag="ACHGMT_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(1813..2814)
                     /locus_tag="ACHGMT_002740"
     CDS             complement(1813..2814)
                     /locus_tag="ACHGMT_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFN6836431.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(2973..3239)
                     /locus_tag="ACHGMT_002741"
     CDS             complement(2973..3239)
                     /locus_tag="ACHGMT_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN6836432.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3229..3585)
                     /locus_tag="ACHGMT_002742"
     CDS             complement(3229..3585)
                     /locus_tag="ACHGMT_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFN6836433.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1158 a          657 c          488 g         1340 t
ORIGIN      
        1 taataaaata aaaagagcga accattatag atcagctctt ttttcattgc tgtttttaat
       61 cttatctaga atcacttcaa atgtttcaaa ggtatttaca tctaactgac ataaccaaag
      121 caagtacttc ttttctttgt aagtccaatc aatattacgg taagaggaca ttagattatg
      181 gtaccttgct ctttgttcaa ctgaagccaa ttcccagcgt ttttcaaata ttttttcttc
      241 ttccattatt gatccctcgc aagacgctac ctcaaactta aataatgggc agcgaaagtc
      301 taattcctta gatctaagta tagcaaacaa atatgaaaaa aacttagaag gtagtaaaag
      361 ttcttccgtc ttttgctttg cttctattct taatgtgttt cgttatcggt gccctacaaa
      421 gattttcatt aattacacac tcatttattg ttgtgtattt tctgacattt ctctcttgat
      481 caattatttt aattccatgg gcatcctgat agttcatagg agaggagcgg ctatcagaaa
      541 tagttgcacc tatctcttga acttctttta caacttctgg aaaatccatc catcctgctt
      601 cagctgtcaa aagtaataat ctataatatt tatctttcct tttcaatcta agaaatcgtc
      661 accccattct ttttcttcta ttataaacta tttaactttt agaattcaaa aaccgtttgt
      721 ctatttagag tttaccctaa atagacaaaa atataacatt gaaaaattta gagttctaac
      781 gtatgattag ggtgtattat aaacgttata gaaaagtagg gaataaattt gaaaattgct
      841 tatattagag tttcatccat cgatcaaaat gaacaacgtc aaattgaaga aatgaaaaag
      901 tttggcgctg aaaaaatttt tatcgaaaaa caatctggag cgacgattag tcatcggccg
      961 attttccagg aagctttaga ttttgtcaga gagcaagata tttttattgt agaagccatt
     1021 gatcgacttg gacgcaatta tgacgagatt atcgattcag tgaattatct aaagaagaag
     1081 aatgtgcgac ttattatcac cagtttaccg atcatggctg aagcaattgg aaatcctctc
     1141 ctagataaat tcatcaaaga cttaatcatt taaatctcag ccatgattgc tgaacaagaa
     1201 cgtactgaat cgaaacgacg ccaggcacaa ggaattaaaa tcgctaaagc aaacggcgtc
     1261 tataaaggtc gtcctaagtt atacagtgca gacaccaaag atcctcaacg tcgccttgtc
     1321 tataggagta tcgttcagga tcttgagaat ggggttgcta tctcaaagat tgcaacagac
     1381 tacaatgtga ctaggcaaac aatttaccga ataaaaaaag aaatcgacca actaattgta
     1441 tgatttcttt tttgaatcac gtaattatta attagctttg tcatcaatac agacaatcat
     1501 tttagattca tctagctaag ttgattacat ttatgttaaa aagaatttag ttaacataaa
     1561 tataattgat gcataaatcg tccttattta aaggcattta acattgacaa cttagtataa
     1621 tgtatcttat gtaaactaat aagatttgaa ttttatttta tacacactcg ataatagatc
     1681 aattttatgg tcccccgcat ttacgattgc acaagagaaa cataaagtcg acctattagc
     1741 atttcttttc aattcaaaca ttaaaaaagt ttatacctct gctactaaaa aatgaaatta
     1801 tctatttaaa atttaaaacg gtaaatctga ttcatccaaa ttggaaaatg gatcagcctg
     1861 tattggagct ttgtatttct ccataataac agatatcttt ttttcatttt ttaaaaaata
     1921 ccttttaaat ttcttatagt cctctgaaat caaaatatag cgtgatggat actcatcaaa
     1981 agaagaatcg tattcttcct ttaaaattcc cgatttactt aatttttcta gtacatcaaa
     2041 tactctatca ctcaagtaac tccttaaatt gttatcactt tcccattgaa ctacttttct
     2101 ataagttgaa ttatcagttg aaaagataga attttcatcc ataaacgcat atattagcag
     2161 caatctttca tcattgtcaa attctattgg atcaaaaata tctaagttgt catcaaaatc
     2221 tttatcagtt ccggtaactt ttttgttaat tactgaaggt gtcttgtaag aaatagaatc
     2281 taaatcaaag cgttttgatt ctaaattatt ttgtgctttc tcaattaaat aattcacttt
     2341 ttttgtgaga aaccttctga tattgtcttt gctatatgtt ccataaaaac ccttatcttc
     2401 taaagagtcc ctgtacttct taacttctat atactcttca gccaaaactt gtggaactgg
     2461 ttcatctaag aaaaaaaccc ctacctcgcc tttgctattt ttataaaagt aatcaatttc
     2521 tgctatagtt cccgatctat aattatcaac tggagaaccc aacttatggc caaaaatcgc
     2581 taataatata tcactatttt ctacaatttg tttattaata acactttgcc cagagctatt
     2641 atttatataa gtaggaatcg cattcgtttc ccatcgaatt ggcatcaaaa ttacttttgt
     2701 attatctgta ttttctatat tccatctata tatggtttct tctacttcat tcctctttgc
     2761 tgctatatca cctggtgatg ctataaatac ttttattaga tttatggttt tcatttattg
     2821 tacctttctc tttatgtctc tttaaagtaa tttttgtata aaatttattg acttttacac
     2881 tataggacaa cttttatcta tttagtacaa aaaggcccac taaactaatt gattagttag
     2941 gagcctatta caggtatatt ttcaactcgc ttttaagcaa attctactac cagctttttg
     3001 cctaatgccc tagcgatatc atcaagttta tcgaaagata cgttatcccc tctctcaatt
     3061 cgagcaatag tagattgagt tgtatgtgct ttttgtgcaa gctccgcttg agttaggcct
     3121 gcttcctccc gtgcatgata aagagcaacc gcagtttcga gttttcttcc ttcacggtca
     3181 tatgcttctg caaaatcttt gtctttttgc ttttcttcaa tcaatttttt aatcatcatt
     3241 tgtgaaccac tcctctctaa gttcttttgc atgtttaagt tcacttattg gtgttttctc
     3301 cgttttttta gtaaatccat gggtaatgac ataccttcca ttgaccacat ggaaatagat
     3361 agctctttgg atgtttgatc ctaccttact tcttaattcg tacaaattag tgtctaattt
     3421 tttcacccat ttcaaccgtt gagctactag catcccattc ttttctgttt cttctattgt
     3481 acgtaaaagc ttcgcactat ctttttttgg taatgtttcc aaccattcta aaaattcatc
     3541 atgaccatta ggtcttttaa atgtttcaaa agatattttt tccataccca attatatcat
     3601 atattgattt tatattatag tatataacat aaaaattcga att
//
LOCUS       DAZBSA010000165         3603 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000165 DAZBSA010000000
VERSION     DAZBSA010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.170"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            116..1447
                     /locus_tag="ACHGMT_002743"
     CDS             116..1447
                     /locus_tag="ACHGMT_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFN6836434.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            1413..1763
                     /locus_tag="ACHGMT_002744"
     CDS             1413..1763
                     /locus_tag="ACHGMT_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836435.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1855..2274)
                     /locus_tag="ACHGMT_002745"
     CDS             complement(1855..2274)
                     /locus_tag="ACHGMT_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFN6836436.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(2275..2619)
                     /locus_tag="ACHGMT_002746"
     CDS             complement(2275..2619)
                     /locus_tag="ACHGMT_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFN6836437.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            2969..3565
                     /locus_tag="ACHGMT_002747"
     CDS             2969..3565
                     /locus_tag="ACHGMT_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFN6836438.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT          949 a          652 c          653 g         1349 t
ORIGIN      
        1 aaaatttggt taaattcaat gggttccctt acatttttag ttttgaccta aatgatagtt
       61 gtccaaatct ttcatttcaa gttatagtta aagaaaatga aaaagaaggt gtgtaatgga
      121 aaacgaaact gacgttattt atattcatcc tcaaaaacga atcgtttcac agaaaagaaa
      181 gtatttttat cttggtttta caggtgtttt ttttcttttt attggcttgc tttctaatac
      241 tcctactgat aattggagtg gattgctaac tattcttacc tcgccaagca atcttttgac
      301 agattatttt gctttaggtg ttttcggaag cgcattcata aatgtaggga ttttgacctt
      361 actttctgtg cttttagctt atcggcacaa ggtgatttta aacggtccct tatttgcttc
      421 gattctaaca gttaccggtt tctctttttt tggcaaaaac ctttataatt ctatctcaat
      481 cattttaggt gtgtatctat acgctgtatt tgtcaataaa cctttttctc agtacatcat
      541 gatcggactg tttggtagcg tattatcacc ggtcgttagt tatatcactt ttggtatgcg
      601 ttttccttta ctgataggta ttttattggg gaatttagct ggcatagcaa ttggtatact
      661 tcttccgccc ttagctgctc aaactttagt tttccatcgt ggattcacac tttataatat
      721 tggattcacc tctgggttga ttgcgatgac ttttactgct gttttgaggt tattcagcta
      781 ttcaatcgtc gaaaatacat tggttttcaa tgaatatcac tttcctctca tttggattat
      841 ttttggcttt ttttcgctga cagtcggaat tggtttctat tataattcct ttcggctttc
      901 ggggattcga gaaattttcg attcatctgg aaaattgaca actgatttca ttgctaattc
      961 tggtattggt gctactttga tcaacatggg cttagttggt ctgatgctgt cttcttatgt
     1021 tttgctggtt ggcggtcagt taaatggacc tgtcatcggt gctattttat ctgcggtggg
     1081 tttcagtgct tttggttgtc atttaaaaaa cagttttccg atactagtcg gaatatttat
     1141 tgcttctttg tttggaactt ttcatgagat caccagtact ggcatgcttg ttgcagctgt
     1201 gtttgggaca ggtcttgcgc ccataagcgg tttttacggc agtttttacg gtgtaatagc
     1261 tggtgtgcta catattgctc ttgtccataa cgtcagcact ttgcacggag gactaaatct
     1321 atataacagt ggtttttcaa ctggtttcgt tgcaggtatc cttgtaccga tactagataa
     1381 ctttaccgct gtcagaaagg aaaaaaagac acttggaaaa agaattatca aaaagaatca
     1441 tcgatgagct tcttacttat ttatattccc atgaaataac tgaaatacgt attggagtag
     1501 aatttgctga ggaagggctt ttcatagaga tacagggtac ggcagaaact cagccagaag
     1561 atttagatgt acttgctgat ttgctgaaca atcctcggga ccctagtatg gaaatctact
     1621 atcaagaact attaggtaat ccccgacatg atcgggaaga ttatcatctg ttaggtagtt
     1681 tgattgatga cgtggaaatc atgtatgccc agccgattat caatattaag atctttcgac
     1741 gtgcttctca ttcctattat taaccctatt ttctatttat aaaagactga atcaaaaaag
     1801 cagctcctga aagttcgatt aaaatctact ttcaggagct gttcaagctt gacattattg
     1861 attttccaat ccttcataca cttcatctgt tacaggttct aaaaactctg aattaccagc
     1921 agtgatcgcc aaatgttcaa accaactgtc ttttgtcgca ccatgccaat gtttaatccc
     1981 tttgtgcgtt acgacgacat ctccagccat taatcgtctt gctggctttc cttcttcctg
     2041 ataccagcct tctcctccgg tcacaagtaa gatttgatag ccgtctgtat ggacatgcca
     2101 gtgattgcga caacctggtt caaaagttac attacctaca ccaacagaaa cttcgggatc
     2161 attcactaac atattcagat agctttgccc ggtaaatact tgagcgtaag cttcgttttt
     2221 ttcacctgct ggaaaaatca cgccattgtg tatttcttcg tgttttgtca tcatttacgc
     2281 ctctttctta tcttcgctat tttcttctaa atagatttct tttgctaaac taaaagctga
     2341 ccaagctttt ggccattcta cataaaaagc taaatgggta atgatttctg ctatttcttc
     2401 actagtaatg ccattttgct tgccaatctt cagatgagct ggcaattgtt caaaatttcc
     2461 tcctgaaatt aatgcagata tggtgatgat actacgctga tgagcagata attcttctct
     2521 ggaccatact tgtccaaata aaacatcatc atttaattct gcaaattttg gtgcaaaatc
     2581 tcctaattga tctcgtcctg cggtttgttt ttctgccatt tctcttattc tcccttcacg
     2641 actgttaggt gattcaacta tggttctaag gcaactttat tttctattgt ttcttcgcat
     2701 ttcattttaa atgtagtctt tcagcctgtc caatgcttag tttattcagt cgatatgctt
     2761 tttaagcata gttgctaacg ctttttttat tttaaaaaaa acaagttatt aaataagtct
     2821 ttagttggat gtttttttga gatcaatctt ttatacttat gatgaactta caaaattgtt
     2881 tgttttctgt catatcaaaa aatggcgcag ttagttgctt ttctagtaaa gtatagttaa
     2941 caaagaacct ctaaggagtt gatgaataat gaatattgct gcctctcgaa aactgataaa
     3001 tttcatatcc attatcggat taggcctatc aatcgcgctg acgatttatt ttattaattt
     3061 aggtgttttc aaagatttga atgcgttaag aggattagta ggtgattcca tcattttagg
     3121 accgattatt tttgtcttca tccaaatcct gcaagtagtt attccaatca ttcctggagg
     3181 gatcagtact gcagctggtg tcctgatatt tggcccttat gctggattta tctataacta
     3241 tattggaatc tgcattggtt cgattatcat ttttctgcta gggcgtcgtt atggaaagcc
     3301 attcatactt tccatgatta gcgataagac atataacaaa tatattggct ggctggataa
     3361 tcaaaaccgt tttgagaagc tgtttgctct agctattttt cttccagttg ctcctgacga
     3421 tgcgttatgt ttgatggctg gattgacaaa catgtcagtg aaacgatata cactaatcat
     3481 tttgattgct aaaccactgt cgattttctt atatagtatg gccttgattt atggaggtca
     3541 atatctaagt ggtcttcttg gataatattt attttgtaaa attagtcatt gattgacaat
     3601 gtt
//
LOCUS       DAZBSA010000166         3549 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000166 DAZBSA010000000
VERSION     DAZBSA010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.171"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..958
                     /locus_tag="ACHGMT_002748"
     CDS             <1..958
                     /locus_tag="ACHGMT_002748"
                     /inference="COORDINATES: protein motif:HMM:NF016640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase domain-containing protein"
                     /protein_id="HFN6836439.1"
                     /translation="NRDEGFMGRQELYSLAINELEKRNLEIHELNQKNNSILGMKIRN
                     KINQLNQMKKRDNLLPAKYTLKSMTGKGPIKDYSNRENRSDELRKKELTNQGTPKRIA
                     VYSCITGNYDSIKEPDFSHPLVDYYLYTDNVITSEHWNIREIPKYISELKNNILINRY
                     IKFHPNELFTDYDFTLYVDGNVTITSDITGLLNSSSKLGLSMHKHSERKSLYSEADAL
                     IAVGKGNKESIIEQISKYREEGFPDNFGLAEATVILVDLNNSIAENLLDLWWREYLNS
                     ESGRDQLAFPYVLWKSGHAMKEVTTLGNNVYKSAKLRIISHY"
     gene            1210..2220
                     /locus_tag="ACHGMT_002749"
     CDS             1210..2220
                     /locus_tag="ACHGMT_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HFN6836440.1"
                     /translation="MKILITGGAGFIGFHLSRKLLKETENQIVAIDNLNDYYDVQLKE
                     DRLTILYDYNNFKFIRMDLLEKEKLDSLFESEGFDIVIHLAAQAGVRYSIENPYAYID
                     SNIIGFLNILEACRNFPVKHLLYASSSSVYGGNTKVPFSTEDSVDHPVSLYAATKKSN
                     ELMAHTYSHLYKIPTTGLRFFTVYGPFGRPDMAYFSFTKNILSGKEIKIFNHGKMERD
                     FTYIDDIVEGITRLINKVPEPNSDWTDIDDLSSESWAPYKIYNIGNNKPVALLEFVET
                     IEKYLGVKANKIFLDMQPGDVVRTYADIEDLEEKINFKPNITLDEGIKKFVEWYTVYY
                     LS"
     gene            2588..>3549
                     /locus_tag="ACHGMT_002750"
     CDS             2588..>3549
                     /locus_tag="ACHGMT_002750"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836441.1"
                     /translation="MDSSSNKSHSIAKKAFIYAIGTLSSKMISVIIIPIYAVYISVAD
                     LGSFDYQNTIAQFLSPVIFGAIWEAILRMGLGKEKNIQKKVVTNSICFVIANTVILAI
                     FLPIIYRMIFPNLEYYQLFVFQLIFTPILSVFQYIVRALGESKIFVMSGIIAAGINLL
                     LLTIFVVTMGMGILGLLLSIIISNVINLIFLIFRGQIFQYFATSSFDFLFLKSMLKYS
                     IPLTLNLTFGWLIVGFSRFYIGTYIGVTENGIFSFAMKFSSIILTLTSIVSMSMIEDA
                     VITSENDDFIERFVEKNTYIFKMYIRLATLSIPIICCAYSFITNP"
BASE COUNT         1234 a          472 c          639 g         1204 t
ORIGIN      
        1 aaatagagat gagggattta tgggaagaca agaattatat agtttagcta tcaatgaatt
       61 ggaaaaaaga aatcttgaaa tacatgaatt aaatcaaaaa aataattcta ttttaggcat
      121 gaaaatacga aataaaatta atcaacttaa ccaaatgaaa aaacgagata atctgttgcc
      181 agcaaaatat acccttaaat cgatgaccgg taaggggcct attaaagact attcaaacag
      241 agagaatcgc agtgatgaac taagaaaaaa agagttaact aatcaaggaa cacctaaaag
      301 gattgctgtg tatagttgta tcactggtaa ttatgattct attaaagaac cggatttttc
      361 tcatccattg gtagattact atttgtatac agataatgtg attacaagtg agcattggaa
      421 tattagagag atacctaaat atatatcgga attaaaaaat aatattttga tcaatagata
      481 tattaaattt catcctaatg agctatttac tgactacgat tttactttgt atgttgatgg
      541 taatgtaaca ataacttctg atattaccgg attactgaac agttcttcca aacttgggtt
      601 aagtatgcat aaacatagtg aaagaaaatc tttatattct gaggcagatg cattaattgc
      661 agttggcaaa ggtaataagg aaagtattat agagcaaatt agtaagtaca gagaagaagg
      721 atttcctgat aattttggat tggctgaagc cacggttatt ttagttgatt taaataattc
      781 aatcgctgag aatcttctag atttgtggtg gagagagtat ttgaactcag aaagtgggcg
      841 agatcagctc gcatttcctt acgtgttgtg gaagtccgga catgcaatga aggaagtgac
      901 taccttaggc aataatgtat ataaaagcgc taaattaaga atcataagtc attattagcc
      961 ctaaatgatt tctaaactat tctttcaaat cctagtaaat atttggagat agaagatttc
     1021 ctttttaaag tcataaaaaa ttaagttata ttttttgagt gaatggagaa gacgcatagt
     1081 atcataaact tcgttatatc ttaaattatg gggtatgcct aaataataaa tgtaatacga
     1141 gtataaagaa gaaagcaatt ttgggtttaa ccctattggt tcctttcaga aaaaggtgga
     1201 tgaataaaaa tgaaaatatt aataacaggt ggagcaggat ttattggttt tcatttgtct
     1261 agaaaattac tcaaggaaac tgaaaatcaa attgtagcga ttgataatct caatgattat
     1321 tatgatgtcc aattaaaaga agatcgatta acgatactgt acgattataa taattttaaa
     1381 tttatcagaa tggatctatt agagaaagaa aaattagatt ctctatttga aagtgaaggt
     1441 tttgacatag taattcattt agctgctcaa gcaggtgtta gatattctat tgaaaaccct
     1501 tatgcttata ttgattctaa tattatcggt tttcttaata ttcttgaagc ctgccgaaac
     1561 ttccctgtaa agcatctatt atatgcatca tctagttcag tatatggtgg gaacacgaaa
     1621 gtaccgtttt caacagaaga ttcggtggat catccagtta gcctttatgc ggctaccaaa
     1681 aaatcgaatg aattaatggc tcatacttat agccaccttt ataaaatacc aacaactggg
     1741 ctgagatttt tcactgtcta tggtccattt gggcgaccag atatggccta tttctctttt
     1801 accaagaata ttttatctgg aaaagaaatt aagattttta accatggaaa gatggaaaga
     1861 gatttcactt acatcgatga tatcgtagag ggaatcacta gattaatcaa taaagtacct
     1921 gaacccaact ccgactggac agacatcgat gatttgagta gtgaaagttg ggcaccatat
     1981 aagatataca atattggaaa taataagccg gttgcattgt tggagtttgt tgaaacaatt
     2041 gaaaagtatt taggtgttaa agcgaacaag atttttttgg acatgcagcc gggtgatgtc
     2101 gttagaactt atgccgatat tgaagattta gaggaaaaaa tcaactttaa accgaatata
     2161 accttggatg agggcattaa aaaattcgtt gaatggtata cagtgtatta tttgagttaa
     2221 ttttgaaagg aatattgaag atgggtaatt catacgattt ttttagcttt gttaggtatt
     2281 ttaggaaatt ctactttcaa acgtgacgaa ctttctcgcc gaatagatat tttctataac
     2341 taaaagaatt tctataaaac aaaattgtgt atcaagatgt gtgaactgga ttctgactgc
     2401 caatattgat tgatgagatt gaagaaatat atagttgatc atggatatct ttatttgaaa
     2461 agatcttagc tattcacata ttcaaaaaaa tttttactac gaggactcac acgatacaga
     2521 ggaatttagg tagttctaat taaaccagaa agtcatctat agatgtgagt tgagggaggt
     2581 catacggttg gatagtagca gtaataaatc acattcaata gctaaaaaag cttttattta
     2641 cgcaatagga acattatcat caaaaatgat atctgtcatt ataatcccta tctatgcagt
     2701 atatatttct gttgccgatt taggaagctt tgattatcaa aatacaattg ctcaatttct
     2761 ttctccagtt atttttggag caatatggga agcaatttta cgcatgggat taggcaaaga
     2821 aaaaaatatt cagaaaaaag tagtgacgaa cagtatttgt tttgttatag caaatacagt
     2881 gatattagcc atttttttac caataatata tagaatgatt ttcccgaatt tagagtatta
     2941 tcaattgttt gtttttcaat taattttcac tcctatccta agtgtttttc aatatattgt
     3001 aagagcacta ggtgaatcaa agatttttgt gatgagtggt ataatcgctg cgggtatcaa
     3061 cctgttactc cttactattt ttgttgttac aatgggtatg gggatacttg gtctcttact
     3121 ctctatcata atttctaatg ttataaattt gatttttttg attttcaggg ggcagatctt
     3181 tcaatatttc gcaacgagtt cttttgattt tttattttta aagtccatgc tgaaatactc
     3241 tatcccactc actttgaatc tcacttttgg atggctaata gttggttttt caagatttta
     3301 tattggaacg tacatcggag taacagaaaa tggtattttt tcttttgcga tgaaattttc
     3361 atctataatt ttaactctta caagtattgt tagtatgtct atgatagagg atgcggttat
     3421 cacgtctgag aatgatgatt tcatcgaaag attcgttgag aaaaatacct atatttttaa
     3481 aatgtacatt aggttagcga ctctttctat tcctatcatt tgctgtgcat actcttttat
     3541 aaccaaccc
//
LOCUS       DAZBSA010000167         3544 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000167 DAZBSA010000000
VERSION     DAZBSA010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.172"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            17..1063
                     /locus_tag="ACHGMT_002751"
     CDS             17..1063
                     /locus_tag="ACHGMT_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN6836442.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            1065..3218
                     /locus_tag="ACHGMT_002752"
     CDS             1065..3218
                     /locus_tag="ACHGMT_002752"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFN6836443.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         1101 a          690 c          699 g         1054 t
ORIGIN      
        1 attaagaggt gtttatatga ctcaaccatt gtaccagaca atatttaatg atttgcaaac
       61 ggctatttta actggtgaat tacctgtaga cagccaacta ccaactgaaa aagaactttc
      121 acgtacctat aaagtaagca gaatcacttc caaacgtgca ttgactgaat tagagcaatt
      181 aggacttatt tatcgcgttc gcgggaaagg cagtttcgtt aaatccgctt ctccggaaca
      241 gattcttgct tctcctacaa aaacacgacg gattctgttt cttcttccat atgtcgctga
      301 tttgtctgtt ggtgatttta gcaagggatt gaaccctgtg atgcaagata aacagattga
      361 tgtcatgatg acgacgcttg actttctaca aaataaaaca gcaaaagagc taatcaatga
      421 atttgacgga ttgatttatt atgctcttga tacagaacaa catcttgact tactttttga
      481 gctttcattg aaagaattcc ctgtcgtcat attagataaa aaaatctatg agttaccttt
      541 tcctaccgtt ctttccgata atttccaagg cggtgcgctt gctgcctctt atttgatcga
      601 acagggacat tcaaaaatcg cttatttgtt tggtacgcag actcatccac aatccgttcg
      661 acagcgttac cttggttacc ttgaagcatt ggacaaagct ggtttgactt tccacacttc
      721 actggatgac aaaagcgcga cattagatga attgctttct tatatcacta agtatgaagt
      781 aactgctttt gtttgtgaaa acgatgttgt cgctatccag accatgaatc tcttgagaca
      841 gcatggttat catatccctg aagatttttc aattatcgga tttgatgata tccaagcagc
      901 agctcttgtt gacccgccac tgacaaccat cgcacaagat tttgaacagt taggtaagct
      961 tgcgggagat gctttggtca cttggttaga gacaaaagat cgaccatccg atagtaaatt
     1021 tccagtttca ttcatcaaaa gacaatcaac aaaggagaaa taaaatgaat atcggtcaaa
     1081 tagatactag acatgcaaca gcgaatcagc caaatttctc taatgggaac tgccagcctt
     1141 atactggcgt tccttttggg atgaattatt ttgcaccgca aactacggat caaaacggca
     1201 gctggtggtt tcatcctgat gaccgcgttt ttcaaggcta ccgtcttacc catcaaccaa
     1261 gtccttggat gggtgatttc agtcatatgc tgtttacgcc gatcaatgga aaactacagg
     1321 agaacaccct tttccatgct caaagttctt accgcccaga agaaagtata ttttgcccga
     1381 ctcatctgtc gatctgtcaa ttacgttatg gcatctgttc tacattgata cctagcatgt
     1441 atggcggaat cttatctatt gattacagca aaaatgacag cggtttactt ttatcctttc
     1501 cgggacgcca tcagctcttt gttatagatc cttacacagt ggaaggaaaa atcacacatt
     1561 ttgctggttc agaagatcct gatttttctt tttattttat attgagattt gaatatcctt
     1621 taacaattga tgagttagct gtttccaaca aaaatagcga ctcgatcccg ttgtattttg
     1681 aacagacaaa aaaacaaact atccgatttg ggacatcttt tatcagctcg gaacaagcag
     1741 cttataacct ggaactagaa caaaaccaaa gcaaagagac ttaccttgct cacagtcaag
     1801 aacaatggca gtcctatttt gatcgtatac agctcacaca ccatgatcaa caacaattgc
     1861 ggacgtttta tcataattta tatcgggtgt ttttatttcc acaaacattt tatgaaattg
     1921 atcctagtgg gcaaaaaatc cactatgata cgactagtcg atctataaaa cctggtgttc
     1981 tctatacaaa taatgggttt tgggatacgt ataaaacggt ttatcctctt tattctttga
     2041 ttgctgtaga aaaatacgaa gaaatgctgg aaggtttttt gaacagctat agagaaagtg
     2101 gctatttgcc aaaatggctg tctcctgatg aacgtggact tatgcctggg acattgatcg
     2161 atgcggtcat tgcagatgct gctgtaaaag atatccgacg agacttgatg cctgaatttc
     2221 taacggcaat ggaaaaaggt gctacggttc aaagcaaaca tccaaattat ggacgccaag
     2281 gaacagaaga ttacttgaca tatggctatg ttcctaatca ctaccacgaa tcagtcaatc
     2341 acacattaga ctatgcttat agcgattttt gtattagcca agttgccaag gtactagaag
     2401 aagaggaaat gacgaagaaa tatcagcagc aatcattgaa ctatcaaaat atctttgact
     2461 atgagtcagg atttatgcgt tcaaaagata aagatggcaa gttcagagaa ccattttcag
     2521 ctaatcgttg gggacaagat tatgcagaag gaagtgcttg gcaaagcagc tttgctgttt
     2581 atcacgattt ttcaggactg atcggaaaat atggtggcag tgagagatat aaagaaaaac
     2641 tcattgagct gtgcaatcag cccccaacgt ttgacccagg aggatatggt tttgagatcc
     2701 acgaaatgag tgagatggcc gcgatagaat ttggacaatt agctatttca aaccagccaa
     2761 gttttcacta tccttacttg ttcagttata taggacaacc tgaaatggca cagccactgc
     2821 ttaaacagtt gatgactcag accttcgacg attctccaat gggttaccct ggagatgagg
     2881 acaatggaag tatggccgct tggtatatct tcaatagtct gggattttat cctgtcacgc
     2941 ctggaaccgg tgaatatgtt atcggtatgc cattaattga caaggctgtt ttgcttttgt
     3001 caaatggaaa ggaattgacg attgaagcaa cgcctaatcg gccgcagcaa caatttatcc
     3061 ataaaatcaa acggatggac agagaatata cgaaattata ttttacccat caagatttgc
     3121 tgaaaggcgg cacgatctct taccaattag ggatcatccc tcaccctaag aaatatcaag
     3181 caaaagattt gcctttttcc ttatcaaact ataattaaca agaggttgtg aaaaagctgt
     3241 cctgcatcaa gtaataagaa cagccaagca aaaatagatc ctcatatttt tcgtttggtt
     3301 ggtcttatta ccgcagatgc aagcttttga acaccgttta ttgagaggtt gtgaaatcag
     3361 cgttctgacc tgagtattag agagacaaat ggcaaaatag ttcctcatat tttgccattt
     3421 tttgagctaa taccgagggt cagctgattg aacaccattt agtggagagg tcgtgaaaaa
     3481 gctgtttagt ttcgagtatt aagaaaaatt ttgtgaaaat ggctttccac atttttgcat
     3541 aaat
//
LOCUS       DAZBSA010000168         3448 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000168 DAZBSA010000000
VERSION     DAZBSA010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.173"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2232)
                     /locus_tag="ACHGMT_002753"
     CDS             complement(<1..2232)
                     /locus_tag="ACHGMT_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFN6836444.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="ACHGMT_002754"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="ACHGMT_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          964 a          762 c          548 g         1174 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac gacgaaaa
//
LOCUS       DAZBSA010000169         3307 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000169 DAZBSA010000000
VERSION     DAZBSA010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.175"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>3307
                     /locus_tag="ACHGMT_002755"
     CDS             <1..>3307
                     /locus_tag="ACHGMT_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836445.1"
                     /translation="PQPINKIDSGFDTLIRVKLKETNLSKGTSVSKQLQQPFYKSLLF
                     LPNITKIVIETTDDEKISEKIVDGNDVLIEKSDVTGQSPSEEYFVFDKESMIGDKVAK
                     IALAVSKDSDYDYSKEKLYCYFPVRNFGTPIHALINAPFITNNSRDDVPDDSEQINKK
                     IFIEVLKFISEVAEKLSKPEYEDIALKMVTPFKENKLWDLDVFDLMNEFLGILSNAQI
                     LPTVNWQYISIKDNPKLFLQEFPEELIGETFSTLLQLLETNENDQLVQELANYNEYTG
                     LSFVVDELSDKINILSSSWENRIRTKVFLWWSEQYKSSPIVPKLLKDNSDNWLMKSDK
                     VFLPTDTGVTVLPKELSSWVSLSILKQTYVDELIYQIKTSSSWKNKWEETANAYKAER
                     TGDKRILDAFSEKYLAIEFTEQSSSDLIIGTINRQIDTVEKSISFINWFYDNYNEKLS
                     AGSELSKVPFNLPNVAGDVQPSNKMYIGKEYGNELGDKLFQDTDYTALKKIDCSSFAE
                     ERDFLSFILRCGVSKYPKIYDNDSLKNDVGFKKFIYNKYKNEIKFTNINYLTSKYISN
                     FEELIDKLDTKDIVNWLNQDEELTDLLSSVKKESSASQQSNWSGLYFNSNEYVKYILN
                     KTAWIKIDGEKYSPRQIVLYEKLQAHVPGLYGVSEQSLMELLGQRIVEQLDFRKNMAM
                     FSDDEIRAILVELPKFDKGEISRRLYNELIRNKKGMNPTYSTEGLSVLAKDGFFYPNK
                     ELRYADKRLPKSIEKQMRFIYVPEKASTTTIYEWLGVERFKTNLKLETFELLEDYSED
                     FKKEIDDIKTAVLSTIDDNSKNVSSLKRIQIIPCSKISAKDVEQDGQSIVLEDYYFVE
                     SKGKFYLKLPANSIEINQLRLIDTFSSTIVDIFKQVLNLSLDLNLIELLISRDTEHKQ
                     RKITEEFGVDKWNESFELLFSENALNKKVNDFLLSNGLNEEEYPTIFEIDYSYALKEE
                     EYQLLAIALESISKDIEDLNGLSELIHLDIREFWKLKLRSYLDKKAEHYRQILYSNAN
                     KSGDEILKETFLEKIDRYKDYQFEISEIPNSVQVDIEKESKKRFPELSVSITDEIDID
                     GLYDNNVEELVTVA"
BASE COUNT         1242 a          441 c          603 g         1021 t
ORIGIN      
        1 cctcaaccaa taaacaaaat tgattctggt tttgatacat taattcgagt aaaactaaaa
       61 gaaactaatt taagtaaagg cacgagtgtt tctaaacaac ttcagcagcc attttataag
      121 tcactgttat ttttaccaaa tataacaaaa attgttattg aaacaactga tgatgaaaaa
      181 atatcagaga aaattgtgga tggaaatgat gtattgatag aaaaaagtga tgttactggt
      241 caatcaccgt ctgaagaata ttttgttttt gacaaagaat ctatgatagg agataaagtt
      301 gctaaaattg cactagccgt ttctaaagat tctgattacg attactccaa agaaaaacta
      361 tattgctact ttccagtacg taattttggt acaccaattc atgcacttat aaatgcaccg
      421 tttataacaa ataattcacg tgatgacgtc ccggatgata gtgaacaaat aaataagaaa
      481 atatttattg aggtgctcaa atttatttct gaagtagcag aaaaattatc taagcctgaa
      541 tatgaagaca tagcattaaa aatggtaact ccttttaaag agaataaact atgggactta
      601 gatgtatttg acttaatgaa cgaattcttg ggtattttat ctaatgccca aatactaccg
      661 actgtcaatt ggcaatatat ttctatcaaa gataatccaa aacttttcct tcaagaattc
      721 cctgaggagc taataggtga gacattttcc accttgttgc aattattaga aacaaacgaa
      781 aatgaccaac tagtacaaga acttgctaat tataatgaat acactggcct gtcgtttgtt
      841 gtagatgaac tatctgataa aatcaacatt ctttcatctt cttgggaaaa tagaataagg
      901 acaaaagtgt tcttatggtg gagtgagcaa tataaaagtt ctccgattgt ccctaagcta
      961 ttaaaggaca atagtgacaa ttggttgatg aaatcagata aggtattctt accgaccgat
     1021 acaggtgtta ctgtgcttcc aaaagagctg tcttcttggg ttagtttaag cattttgaaa
     1081 caaacatatg ttgatgagtt aatttatcag attaagactt catcaagttg gaaaaataaa
     1141 tgggaagaaa cagctaatgc atacaaagct gaacgtacag gagataaacg tattctagat
     1201 gcattcagtg aaaaatatct tgcaattgaa ttcactgagc aaagcagttc agatttgatc
     1261 attggaacga ttaatcgaca aattgacact gttgaaaaat ctatcagttt cattaattgg
     1321 ttttatgata actacaatga aaaactatca gctggaagtg agctgtctaa agttccattt
     1381 aatcttccaa acgttgctgg tgacgttcaa ccttccaata aaatgtatat tggaaaagag
     1441 tatggcaatg aattaggaga taagcttttt caagatacag attacacggc attgaaaaaa
     1501 atagattgct ctagttttgc tgaggagaga gattttctgt catttattct tcgatgtggt
     1561 gtttccaaat atcctaagat ttatgataac gattctttaa aaaatgatgt tggatttaaa
     1621 aagtttattt ataacaaata taagaatgaa ataaaattta cgaatattaa ttatttaact
     1681 tctaagtaca tcagtaattt tgaagaatta atagacaaat tagatacgaa agacattgtc
     1741 aattggctca atcaagacga agaattgaca gatttattat cttcggtaaa aaaagaatca
     1801 agtgcaagtc agcaatcgaa ctggagtgga ttgtacttta attcaaatga gtatgtgaaa
     1861 tacattttga ataaaactgc atggattaag attgatggag aaaaatatag cccgagacaa
     1921 attgtgctat atgaaaagct ccaggcacac gttccgggat tgtatggagt ttctgaacaa
     1981 agcttaatgg aactcttggg gcaacgcatt gttgaacagt tggatttcag aaaaaatatg
     2041 gcgatgtttt cggatgatga aataagagct atattagttg agttaccaaa attcgataaa
     2101 ggcgaaataa gtcgcagatt atataatgaa cttataagaa ataaaaaagg aatgaatcct
     2161 acttattcta ccgaaggatt atcagtctta gccaaagatg gcttctttta tccaaacaaa
     2221 gaattaaggt atgctgataa gcgactgcct aaatcaatag aaaaacaaat gcgttttatt
     2281 tacgtgcctg aaaaagctag tacaactacg atatatgaat ggcttggggt tgaacgcttc
     2341 aaaacaaatt taaaattgga aacatttgag ttattggaag attattctga ggatttcaaa
     2401 aaagagattg atgatatcaa aactgcggta ttatctacga tagatgacaa tagtaagaat
     2461 gttagttcat taaaacgtat tcagattatt ccatgcagca aaatatctgc taaagatgtt
     2521 gagcaagatg gtcagtcaat cgttttggaa gattattatt ttgtggaaag taaaggaaaa
     2581 ttctacttaa aattacctgc taattctatt gaaattaatc aattacgttt gattgatacg
     2641 ttttcttcaa caatcgtaga catattcaag caggtgttaa atctaagctt agatttgaat
     2701 ttgattgaat tgttaatttc ccgagatacg gaacataaac aaagaaaaat aactgaagaa
     2761 ttcggtgtag ataagtggaa tgaatcgttt gagctattgt ttagcgaaaa tgcgttaaat
     2821 aaaaaagtta atgacttttt gttatcaaat ggactaaatg aagaggaata tcctaccatt
     2881 tttgaaattg actattcata tgcattaaaa gaagaagagt atcaattgtt ggcgatagct
     2941 ctagaaagca tttcaaaaga tattgaggat ttgaatggat tgtcagaatt gattcatttg
     3001 gatattcgtg aattttggaa actgaaatta agatcttatc tagataagaa agcggaacat
     3061 taccgccaga ttttatatag caatgcaaat aaaagcggtg atgaaatact caaagaaaca
     3121 ttcctagaaa aaattgatag atacaaagat tatcaatttg agatttctga aattccaaat
     3181 tcagttcaag tcgacattga gaaagaaagt aagaagagat ttccagagct aagtgtgtca
     3241 attactgatg aaattgacat tgatggcctg tatgataata atgtcgaaga gttagtcacg
     3301 gttgcag
//
LOCUS       DAZBSA010000170         3307 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000170 DAZBSA010000000
VERSION     DAZBSA010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.176"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(87..1034)
                     /locus_tag="ACHGMT_002756"
     CDS             complement(87..1034)
                     /locus_tag="ACHGMT_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFN6836446.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
     gene            complement(1039..1791)
                     /locus_tag="ACHGMT_002757"
     CDS             complement(1039..1791)
                     /locus_tag="ACHGMT_002757"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFN6836447.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            complement(1792..2700)
                     /locus_tag="ACHGMT_002758"
     CDS             complement(1792..2700)
                     /locus_tag="ACHGMT_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HFN6836448.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            complement(2700..>3307)
                     /locus_tag="ACHGMT_002759"
     CDS             complement(2700..>3307)
                     /locus_tag="ACHGMT_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN6836449.1"
                     /translation="IQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNFFVSNKKTN
                     GCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETGIVGTLSVD
                     VVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHDNNYSHTII
                     EDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
BASE COUNT         1086 a          617 c          410 g         1194 t
ORIGIN      
        1 tctatcccag atttgatttt acaaaactca tcagtcctta ttgacaaaga gcctgaattt
       61 accctctaat cttatcttag tatgttttaa ttgatttcag ttttcaaata attagtcatt
      121 ccacttaaaa taccatattt cggttgatag ccaattgact ggatcttagt aatatctgct
      181 agagagtcat ggatatcacc cgaacgctct tctttgtatt caagtgtcaa ttctacaccg
      241 agaatttcat tgattgcatg aatcaattcc aaaagtgtgg ttgctttacc cgttccaaca
      301 ttgaattgtt tccccagtgc agcttcctca ttcgcaacta aaagtaatgc ttggattaca
      361 tcatctatat aaacaaagtc tctagactgg cttccatcac caaataatgt aaatgaagtt
      421 gcttctccta cgatttgttt cttatagcga tctacgagaa tagaaatcac accactgtat
      481 ggagaattcg gattttgatt tggaccatag acattgaaaa aacgaactgc gctaccgggt
      541 acatcatata aatgacaata atctagcaca tattgttctg cagcgaattt atcaaccgca
      601 taaggcgtca atgggcgaat cacggactct tctttttttg gtaaagtcgg ttcatcccca
      661 taaacagccg ctgaagaact aaacacgatc cgttttaaat caggttgata cttgcggatc
      721 aattctaaaa tcattaatac actttcaaaa ttcacacgat gtgtttctac tggtcgggca
      781 actgaatctg ccacactagc aactgctgct aaatgaaaga tataatcaaa tttattagct
      841 ttcatgatct cttccatcaa ggatttctca gcgacatctc cctcaataaa agtgatattt
      901 ttcgtcatat caagattttc tacttttccc attgataagt catcgacgac gattaccaca
      961 tgttcttctc ctaaatgatt ggctaaggta gaaccaatga agccagcccc accggtgata
     1021 agataacagc tcatcctttt acctccgata taaataatta tagatatctt gaagctccga
     1081 agaatcttct aaaaatttag gaaacaaaaa ttctaaccat ttaaaatctt cttcactatc
     1141 aatatctaaa attagataat ctctcatttt tattatccca cattgcccat caaaaatata
     1201 attacttttt tttagaaatt caggtttaaa agaatacatt gaggcattca tttcaaaaac
     1261 ttcaggtgtc tgttgcctag atgtgtatct tgatttatta actatagtaa cttcattttc
     1321 aattcttttt accatattaa aatagggatt acgtcttgca gaaacaactg aaaagtagac
     1381 gtctttatct aaatttttta gatgttcatc tattaattct tcaagattag aaacttcacg
     1441 taaaggtgaa gtcaaatcta aatctataat taaatcaaat atattatctt ctctttctag
     1501 ttgtaagaat gaatcacgta tcacttcaat tttccctaca gtatctgtag ctaattcagg
     1561 tttacgttct acaattttca cagacggatt taacttagtc gtcaattcta ctaactcatt
     1621 actgtccgta ttcaaacaag tcgtgcaatt atgttcagga tgtttttgtt gatagatatc
     1681 tatcattgct aaagcgaaat ggactagggg ctttcctgcc aaatatttca aatttttatt
     1741 tttaaacccc ttagacccag ctctaccaca aatagtaaat aaaatttcca tttatttctc
     1801 ctcagctatt ttcaatactt cgtttgcatg atataaatca ttccagtttt ctttgcctgt
     1861 tactacgttt tcaaagaaat atttcaactc cttctcatac atatcattag cagattcagg
     1921 catttcttta acaatgtttt cttcaaaact tattttatta tttaataagt caacattgat
     1981 acattcatct aaagtaaaca attgaaattt tcttgtagga actcttccaa aataatctaa
     2041 atgaatttct ataattttat catcgtatct caatatatat gttgctaaat cattactgtt
     2101 aatttcaaga ttcgaatatt taccaaactg tttttttact gatttaggga aaccaaatag
     2161 gtttataata taatccaatt catgtataca gtctaattct actcctcccc ccatagattt
     2221 aaaggcagaa taattatttc tataatcagt accttttctc caatcaggaa gataagtaga
     2281 acaaatagct cttacagaat atactttttt actttgaata ctattacgaa caacttccat
     2341 gatattttta tacctcagtg gagcagcaac ataaatattt ttgtaatcat aattatattg
     2401 agtaagatca atactctcag aaaaagcagg tttttctaaa aatatgtaat caactttttc
     2461 aacaattaca tttagtgttt ccagatgctt aaaagtcgga tttgtaataa aagctatatc
     2521 ataatgtgta tccaaatcgt ctctattttg aatatacgtt atttcttcaa actctttttt
     2581 tccactgcta ctataagctg ttatatcaaa attaatagaa ttcttttttc catagcttat
     2641 tagatttttt agatgtctct ttccaattga ccctaaccca aaaaaaatta ctttcatcat
     2701 taatttacct caatgtcgtt aattaagtta ataatatact tatctttttc aaaagtatat
     2761 tggggttcca ttttcttttc caaaagcgtg aaaaattctt taatctcctc taaatatgca
     2821 tcttcaataa tagtatgtga ataattatta tcatgcataa cctcatcgta caattgaata
     2881 tctgtcatac tagaagtttc ttcattccat gaaaatagag aatccgggtt accttcccat
     2941 tctattaaaa tcttttcacc aattatagtt aaatttcttt tagctactct tgaaacaaca
     3001 tctacactta aagttccaac tataccagtt tcatgctcaa taataagagt gaaactgtcc
     3061 gaataatcaa tatcaagatt ggataatttc tgggtcatta cagtaaacga tttaactttc
     3121 ccaaatgtat ctataatcca aggtaattca atagcgaata tttctctaca cccattagtt
     3181 tttttgtttg aaacaaaaaa attgcgataa tcttcccaag gatgccagtc gggtaaatac
     3241 tgtcctacat ggtattgata agaaattttt ttattatgcg tcactttaga cttaatatat
     3301 tgaattt
//
LOCUS       DAZBSA010000171         3299 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000171 DAZBSA010000000
VERSION     DAZBSA010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.177"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..284)
                     /locus_tag="ACHGMT_002760"
                     /pseudo
     CDS             complement(60..284)
                     /locus_tag="ACHGMT_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(838..924)
                     /locus_tag="ACHGMT_002761"
                     /pseudo
     CDS             complement(838..924)
                     /locus_tag="ACHGMT_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2090..3130
                     /locus_tag="ACHGMT_002762"
     CDS             2090..3130
                     /locus_tag="ACHGMT_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFN6836450.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT         1229 a          457 c          471 g         1142 t
ORIGIN      
        1 ggtattttta ttatatatag atttaacata tgtcggtcgg ttgattgata tagctaaagt
       61 tataactcaa ccgaagccga ttcatctaca cgaataaagt tgcgagtgtt ctcggctaat
      121 tcataaaaat tgttatgtgt tccatatagc aatactggaa taactttatt ttcttgaatc
      181 tcataagcaa ctctatacac ggtgaccgca tacttaaatc cattagacca attacctgat
      241 aaacctccag ttttttgctc ttacttaaat ggatcaatgg caatttcatc acaaacctat
      301 agaggatgag tgacttctgc ctaattacgt taaaaaatat tgaaagtaac tctatgtagc
      361 aaaaaatatc tagaatttca tttcctcaaa actgatcgat attgtatcta aaaatatata
      421 ctacctaaaa taggtagtat atattaaaat atgtgctttt gttagaaaaa tcaatacata
      481 aattatcata tctgtatatg tcatttaggt atttcagtta tgttataata gttttgctgt
      541 ctgctacccc ggcctaatct ggcaagggga ggaggagata taatgaaata cctttttgat
      601 tttgtttttg caatcaagcc acttgcagat gtagtgtgca gttacctctg caaatggctt
      661 gattgcaaaa ttgccaaaaa agacagtaag cctacccgtt aattctataa tatagaaacg
      721 taaaaaaaac cccatcagcc tgcacgctga tggggtttgg tgatataatg aaatacctct
      781 ttgttactca taattatacc ataattagtc acgaatgcaa ccaatagaaa aaactactta
      841 gaacggtaga tcatcgctaa taacaatgca ttgtccacca aatggatttt gtgaagcatt
      901 aaatacattg ttattaattg tcgctgcttt taactaggcc tctccccttc tattataaaa
      961 gaagatatat atcatttctt aattacctac taccttaaac caaaatttaa gtactaccta
     1021 atttaagtag tacttaaaca atctaaaaaa tagttatccc aattgttatg cttttataat
     1081 acgattattt gtaacaaaat gaatattata taattaaaca ctaccatatt taggtagtgt
     1141 ttaattatta gcaaaacaaa aaatatttat tctattctct taacttcaac taagttatgc
     1201 tgcacacctt tatataaatc atatgcaact cattagctag acaaactaca agcatgtagt
     1261 tttatctgtt aattatgtac tacctaaata aagtagttta taattagcaa gtatatatat
     1321 ttaaaaattt aagattaatt tttttatgac ttgtacaaat ttatctgata ttctcttatt
     1381 aagaactacc taatttgggt agttcttatt ttttacattt taagtagtat ttaattgcaa
     1441 tttggtagta tttttttatt atcttattct tagaacggta gtacttaata taaatatagg
     1501 tagtatctaa tttaagtatt aatggtagta tctaaattaa caggtagtat gtaactactt
     1561 taaagtaatt tttttcagat taacacagta gtatcttatt aaataagtta attttaatgg
     1621 tagtacataa ttattatata tatctgacat tggtagtact tattgtattt tttagtagta
     1681 tataatctat tgttttctag cctaaagcct tgtcttataa gaataatata atatatacta
     1741 ctgaaataag gtagtatata ttactagtaa tggtagtatg ttttatatgc ataaggtagt
     1801 tcttaattta ttttttgttt tgacactggt agtacttaat tatctttaat tttaaaacta
     1861 aattttattc atacgttttt ctggtagtat ttaattataa ataatattta tttaaagtag
     1921 ttcttaatga ctttaaaggt agtacataat tcattttatt tctgatttta ggtagtattt
     1981 aattaaaaaa cagttttaat gcgtttgcat tttttattta tctttgctat tttcgaaaag
     2041 agtaggtaat tttacataaa taaaacaacg tgaaagtaga ggatctacaa tgactgattt
     2101 taaatttttt aaggcggata gagtttacaa cgaattattt tatcaatttc caaaagtctt
     2161 tattgtttct gacgaataca aaaaaatgaa agattcaact aagattgcct atatgctttt
     2221 aaaagcaaga ttagagatcg caatcagcaa acggcaaatc gatgaagaag gtaatgttta
     2281 ttttacttat acgacaaatg aactatgtag agtattaaac tgccaaaaac aaaaagcgat
     2341 agcaatcaaa aaagagttgg aatcctttgg tttattatta caaaagcaga tgggatttaa
     2401 caaacagtta gggaaaaata atcctaatag actatatcta gcagaattaa aagtctcaga
     2461 aaatgatatc tacttactcg aaaaatttga tagagagaat agggaaaacg ttgataaatc
     2521 agagggtatg aaaatcatac ccaccctcga cgaaaaatca gacgctgaat cccttggggc
     2581 tcaagagggt atgaaaatca taccgtgcca aaacgttgat aaatcagagg gtatgaaaat
     2641 cataccagaa cttaataata atatattaga cactaataga cacaatatag acactgaaaa
     2701 agaccgccta caagatcaat tgttgttaga caattttgag acaattatga caaacgacag
     2761 cattgctacg tttgtccctg aacgagtatt aaatttgata aaaacatttt cttcaagtta
     2821 cagtgaagct caaaaaaccg tccagactat tcataatgca aagaaaaaag ctgaaataga
     2881 aagtgatatt tcgatagttt ttgaagaact cgatagttat tatgtcaatg cagaacaaga
     2941 attatacacg acactgttaa aagcctatca aaaattaaaa accgaaaaag tcgaaaatat
     3001 ccagaacctg atttttgtct atgtaaaaaa ttggtttatc gaaaaaccaa tagctgctaa
     3061 agtatcaagt gaaaaacgtt tgaattatgc aagctcccca agcactatta cgaaagactg
     3121 gttagagtga aactttactg acaaagtgat tgttgcttca tggagtaaca ttaaaattaa
     3181 aaatttttta ttattctaat tatattacga atattacaaa ataatagcta ttttaataga
     3241 atttataagt taaaatatta tgataaaaat cacaaacgta gccttttacg tcataagtg
//
LOCUS       DAZBSA010000172         3256 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000172 DAZBSA010000000
VERSION     DAZBSA010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.178"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(139..1338)
                     /locus_tag="ACHGMT_002763"
     CDS             complement(139..1338)
                     /locus_tag="ACHGMT_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFN6836451.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(1468..2844)
                     /locus_tag="ACHGMT_002764"
     CDS             complement(1468..2844)
                     /locus_tag="ACHGMT_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6836452.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2844..>3256)
                     /locus_tag="ACHGMT_002765"
     CDS             complement(2844..>3256)
                     /locus_tag="ACHGMT_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFN6836453.1"
                     /translation="SLVFQNYNLIDYLSPIENIRLVNKSADESILFELGLDKKQIKRN
                     VMKLSGGQQQRVAIARALVSDAPIILADEPTGNLDSVTAGEIINILKTLAKDRNKCVI
                     VVTHSKEVADSADIILELSGKKLKKVNKMNLEVE"
BASE COUNT         1003 a          615 c          460 g         1178 t
ORIGIN      
        1 tcggagtaag tggcttcgac gattggataa ataaattcaa aatcaagcgc ggcatcaatc
       61 tttcgaacga gatgatcttg aggaactaga tcatcaatcg taagcatcgc aaattgattt
      121 ctaccattcg tattgttttt agacatcata gctatcactc cttgcattcg ttttcataaa
      181 tctattataa aagaaaagac tgtagacatc atccataaaa tggactttgt ctacagtctg
      241 cgcattctca ttagagaaca ctcccttttt tgtgactttt ctgaagctag agtgaatact
      301 ttcaactgca ttagtagtgt acatgatttt tctaatggcg ctgccataat caaagagttg
      361 ttcaacatga gaaaagtttc gtttgcatac atctactgca ccagaataat gagaccaccg
      421 attttgaaaa ctgtcgaagg cagcgtgtgc agcgtttaaa gaagaagcac tgtagaattt
      481 tttcatgtct cgacagactt ccttataatc cttacttggg aggtatcgta acgcatttcg
      541 aacaagatga acaatacagc gctgcacaat gacagaagga aaaattgctt ttgcgccttc
      601 tttgagaccg gaaacaccgt ccatcgaaat gaaaaaaaca tcttctacac cacgtacttt
      661 cagttcatca aatacttgca tccagcgatt tttagattct gtttgattga gccataggcc
      721 taaaatctcc ttgtttcctt taagatcata gccaagaatg gtgtacacag catattcttt
      781 agcttcatag ttttcgcgta aagtgacgta catacaatca acgaatagaa atgcataaca
      841 cttggctaat ggccgagctt gccattcttc caattcagga aggacggcat ccgtgatatc
      901 agaaatcatt tcgtgggaaa tatcaaagcc gtaaatatct tcaacggttg cggcgatatc
      961 gcattgactc attcctcgtg catacatgga aagaacctta ccttcgatgt cggagacatc
     1021 tcgttttcta ttaggaatca actcaggttc aaaagaagcc tcccggtctc tgggaacatc
     1081 aatgtttact ttaccaaagc tcgttttaag cgtcttattg ccataaccgt ttcgtcgatt
     1141 gtcatgtttc tttggctctt tagaatgggc gtcataccct aaatggttat tcaattctcc
     1201 ttgaagcatt ttttcaaata agggaccaaa cacatctttt aaaacatctt gcatatcctc
     1261 tacagattcg ggttgataag tattcaaaat ggattcagct aacttctcag catcaggatt
     1321 tcttttcttt ctagccatcg tgtaatcacc tcttgatctt attttagaaa aataaaaacc
     1381 gtgaagcaac cacccgccta ggattgatgg ttacttacac ggtttatatt acactctcat
     1441 ttttggtgct aaaaagcacc gaaaatttta attattgtca attaataatt cttttggatt
     1501 ccttcttaat atattgaatg aagaaataat caatgataca agaagtacta aactcataaa
     1561 tataactaca taaatcatga atttaggtaa tacattgata tctaaacttg ataatgtctt
     1621 gttaaatcct tcagcttctg ccccaccacc taattggcta gatgcagatt gtttagctat
     1681 ttgtttagct atatcaccag taaccttatt caatatatta ttcccaagct tgtcagctgt
     1741 atattgagct aagaaatagg aaccaacgaa tgcggggatt gatataaata ccatctcaat
     1801 taggaattga ccaaaaattt ctaattttga tatacctaat gatagtaata ctgctatttc
     1861 tttctttcta gcattcatcc ataagaataa taataatgaa actacaactc ctgcaaatat
     1921 taatgagcca aaaaataatt tatttgaaat tgagtaaata cctgatatag attgttgtaa
     1981 tgcagggtaa tttgatgaac ttttaatcaa attatattct ctccaattta tatctaattt
     2041 cccaagatct tttattacac tatcaagatt cttatcacct tttacaaaga atgttgcatc
     2101 ttggtataca gctgtatctt cggtattacc ataaacttta gcagctgtat gaatatcagt
     2161 gattaatgta ttttcgtata gttcttgtgc tgcacttact ccaccgctat tatgcccatc
     2221 gaatagacct ttaatttcta cttctactgt ttcatctgca cctttctcat tatcagcatc
     2281 aaataaatta gattttattt ttatcttatc tccaacttta aggttatttt tttcagctaa
     2341 atctttatgc attaagattt tatttttatc ttcattttct aaatgctttc cttctactaa
     2401 tttatatgct tctgatacaa attttgtttc ttttgttgag tcgttaaccc cagttaacat
     2461 aactgttctt ttaaaattct ttgctctttc aggtgattga ttcgcaagag tctcttgagt
     2521 ttcaataata tcataatcaa ctaaatctgc aacactgttt attcttttta cataggagtc
     2581 tatactatct gtttgagata tctttttaat atcttcacct tttacatttc ccccacctct
     2641 aggtgttcct ggatttactt gtctatttat ctccatagaa aaactatttg ttatattagc
     2701 aaatgtttct tttgaagctc tgtccgtagc atctttaata gataaactaa tgagacttaa
     2761 agttgacata gataaaataa ctaatatgat aattaatgat tttaaacttt ttctagttac
     2821 ataagcaaat gcatttttta tcattattca acctccaaat tcattttatt tacttttttc
     2881 aactttttac cacttagttc taaaatgata tccgcagaat ctgctacttc cttactatgt
     2941 gttacaacta ttacacattt atttctatct ttagctaatg tctttaatat attaattatt
     3001 tctccagcag taacactgtc tagattacca gtaggctcat cagctagtat tattggagca
     3061 tctgatacca atgctctagc aatagctact ctttgttgct gtccaccaga taatttcata
     3121 acatttcttt ttatttgttt tttatctaaa cctagttcaa acaagatact ttcatctgct
     3181 gatttattta ctaatctaat attttcaatt ggtgataaat aatctattaa attatagttt
     3241 tgaaatacta aagata
//
LOCUS       DAZBSA010000173         3235 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000173 DAZBSA010000000
VERSION     DAZBSA010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.179"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            121..690
                     /locus_tag="ACHGMT_002766"
     CDS             121..690
                     /locus_tag="ACHGMT_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN6836454.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(868..1308)
                     /locus_tag="ACHGMT_002767"
     CDS             complement(868..1308)
                     /locus_tag="ACHGMT_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFN6836455.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            1466..2230
                     /gene="map"
                     /locus_tag="ACHGMT_002768"
     CDS             1466..2230
                     /gene="map"
                     /locus_tag="ACHGMT_002768"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFN6836456.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            2262..3185
                     /locus_tag="ACHGMT_002769"
     CDS             2262..3185
                     /locus_tag="ACHGMT_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFN6836457.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
BASE COUNT         1031 a          554 c          650 g         1000 t
ORIGIN      
        1 acttgctatt tcagcttgtg aagaaaatca aaaactaatt ttgcatatca aaaaaaacat
       61 gttacaatga aattaatcaa tggtggaagc gttataaaaa aattaaggag attaaaaaat
      121 atggcaagaa aaaagacgat tactagagaa cagattttgg cagctgctta tgaagtagct
      181 gccaccgatg gatttacacg atttactgcg agaaacatcg cgaacaaaat gaattgttcc
      241 acgcaaccga tttatcttga gtttaaaaat atggatgact tgagacaagc tctttttgag
      301 cagatctatg atcatttaca atatgaagtg ttcactgttg agcatacagg gaatacgatt
      361 atcgatcttg cttttaatta tatccatttt gcaagaagag agaagaagtt gtatcgttca
      421 ctgtacttag aagaatatgg tggaggaaaa gagatgcaga atttctctta tacttacttt
      481 gtgaatacag tgaaacgcga tccagcttat gtgaatcttt cagaagagca gatcgaatct
      541 ttgtataatg ggacatggat catagctact gggatcgcag ctttgatgtc ttcaagcatc
      601 atccatccaa ctgatgaaca gattgaaaag atgatccaag acagtataga tgctatcttg
      661 aaattcaaag aaccaatcga aatcgattga atatactcaa aagttccaca aatcagtagg
      721 taacaagaga tgtgtcaaaa taaggaaaag ttcgtttttc ttacagcgta tgcattttta
      781 actcccgaag aactcttcca tgaatgcaat gaataaaaga gccatacaga ttgaataaat
      841 ctggatggct cttttattgc aagtatatta aatttttgcg actagttctt tagcgaaagc
      901 ttctaaattt tcgatatctt cttcttcagc attcagatcc actttaacat tttcggctcc
      961 ttttgatgcg cctgtttttg caaaagcagc atcgaaatca tccaccgatt tgcagaattc
     1021 atcgtaaaaa gtatcaccag atccgcagac accgtaaatt tttcccaata gatccaattc
     1081 ttgcaagtct tcataaaagt ctacgatctc atcaggcaga tcgccatcat cgtatgtata
     1141 agtagctact acgcaaatat ctgcctcctc gaattcatct gcatctactt gggtacattc
     1201 gttgatttcg acttcgatat ccagattttc aaatgcttct gcaacaatat cggcgatttc
     1261 ttcagtgttt cctgtcatgc tagcatatac gatttttgct aaagccatga tgtgcctcct
     1321 cgtttataat tatttaacaa ttatgaatta taccaaaaca tcattgaaaa gaaaataaaa
     1381 cctagagaca cctggggaat gtcatttggc taaatgtatg ccgctatgtt aaaatgagag
     1441 ggatttgtaa aaaggagacg aaaatatgat tactttaaaa tcagcacgtg aaattgaaat
     1501 gatggcagaa tctggagctt tattagcaga tgtacataaa aatctgcgtg attttataaa
     1561 accaggaatc acaagttggg atattgaggt atttgtcaga aactatatcg agagtcatgg
     1621 cggaattgct gcgcaaattg gctttgaagg gtatgaatat gcgacttgta tcagtataaa
     1681 tgatgaaatt tgtcatggat tccctcgtaa aaagcctttg aaaaacggtg atttggtaaa
     1741 agtcgatatg tgtatcgatt tgaaaggcgg catttctgat tcttgttggg cctatgtagt
     1801 tggagaatct acacctgaga tcgaccgatt gatggaagta acgaagaaag ctttgtacat
     1861 cggtattgag caagcacaag tagggaatcg gattggtgac atcggtcatg ctatccaaac
     1921 ttatgtagaa ggcgaaaatc tagcgatcgt tcgtgatttt attggtcatg gcgttggtcc
     1981 tacgattcat gaagaaccag cagttcctca ttacggagaa gctggaaaag ggttgcggtt
     2041 gaaagaaggc atggttatca cgatcgaacc tatggttaat accggaactt ggaaaatgaa
     2101 aatggatcct aacggctgga ctgcgtatac aagagacggc ggactttctt gccaatatga
     2161 acatacattg gctattacta aagatggtcc aagaattttg acatcacaag gagaagaagg
     2221 cacttattga tctatccagt caataggtac gggagaggta catgaaagtt tggaacaaaa
     2281 taaaacaaaa tgaaaatctg atgcgcttta ttgagacgac tcaaaagcgc atcactgatt
     2341 cagaaatgag tacgacctct gtcgtcgttg cttattattt actcttgtct ttatttccat
     2401 tgattattgc ttttggtaat attttaccgt ttctccacat cgatcaagag actgtactga
     2461 cctatttacg agaagtgatt cctgagaccg tttaccagtt tatcggtcct gcaatcaaag
     2521 accttttgac acaaagttca ggaggcttgt tatccgtttc tgcattagcg gctttatggt
     2581 ctgcaagtca aagtatcaat gctttgcaaa ttgcgatgaa taaagcgtat ggcgtagaaa
     2641 atagaaaaaa ttttttgatc gtccgtttct tctcactaat tgtcattgtc ttgttcatga
     2701 ttgctattag tggggtaact atcgttcttg ggttgggaca gctgattttg gatgctattc
     2761 agccaatcgt acaaataccc gtgaacttta tcgatcagtt ccaaactttg aagtggccaa
     2821 tcacattggt agctttattt gtcatcatgt tcttgattta tttaattgtc cctaatgctc
     2881 agttgaagtt gaagacgatc attccgggca cgatcttcgc tactgtcggc tggatgctgt
     2941 tatcacaagt gtttagtatc tatgctaggt attttgcaac gaggatcagc ggttatcaga
     3001 tcataggaag ttttattgta ctcatgcttt ggcttaattt tgctgctacg atcatcattt
     3061 tgggcggaat catcaatgca gttgttcaag aatacgtgac gggtcatcaa atcaaaaaac
     3121 gtcgagatcc aagccgaaaa tggttcaata aattgaaaac taaattttct cataaaaaaa
     3181 agtagaaaca agagttctta ttgtaaattt atatcagtaa tttatttgaa atttt
//
LOCUS       DAZBSA010000174         3170 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000174 DAZBSA010000000
VERSION     DAZBSA010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.180"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1400)
                     /locus_tag="ACHGMT_002770"
     CDS             complement(<1..1400)
                     /locus_tag="ACHGMT_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFN6836458.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNS"
     gene            complement(1464..1823)
                     /locus_tag="ACHGMT_002771"
                     /pseudo
     CDS             complement(1464..1823)
                     /locus_tag="ACHGMT_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            complement(2001..2321)
                     /locus_tag="ACHGMT_002772"
                     /pseudo
     CDS             complement(2001..2321)
                     /locus_tag="ACHGMT_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2437..>3170)
                     /locus_tag="ACHGMT_002773"
     CDS             complement(2437..>3170)
                     /locus_tag="ACHGMT_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836459.1"
                     /translation="LCSKCNSFFGSEYVEEYIRFAEDNKNFLYNNTSLKNGKSDLTHS
                     IKKMNSLRVAKAIVGMFFSLNGDEDSMDKPFLDSVREYLSNPKSTLFPHEDYKIIMNY
                     YLPIGAPQNSKIKIPNDIVNDGDTHNVIKGFLPLDLSTGRKIRFSEIQYEGIGFTLID
                     LKNSTFEIKEGLDLKNLLCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDL
                     LIRWQVITNFLMELKKYSNTQTKFS"
BASE COUNT          991 a          582 c          424 g         1173 t
ORIGIN      
        1 caagaattca aaaagataga cgttatttga agataataat agtcaaattc tatttgaaaa
       61 tctctctcta tttccttaac atactgtcta aatcgaatct cttgataggt gggatatgtt
      121 aaagctgaat tattttggat atcaacattc ttttgtctaa aatatctatc atatttctca
      181 aaaagctgct gcttatcagt gtctttcttc cacgtttgat aaagatactc tataagttcg
      241 ttgatattaa aaacttcgtc tactttttcc aaatcatcca tttgaatata ttttaattca
      301 ttctgtcttt tcataacctt catgccgttg aaattcaccc attgtggtaa atgatacatc
      361 tgaatataaa gttcttccaa atcatctaag aaataaaagg cattggcaca ggtcaattga
      421 atgatatttt tcaaattgcc aacattttca ctaaagcgat agtttaataa aaaatctaat
      481 aattgttgag atatcagtat tttctttgct agtctttctt cttctgattt aaaaaatatc
      541 ttgattaact gttttctttc ataaagagaa cgactgtcta atgttggaag tgttagcttt
      601 acaggtattc ttctcaacaa agttgataat agtttatcct caggtggttc tgatgttgca
      661 aatataatcc agcaacttga tgtgtaccag ttttcattat cacccatccg atgaaatttc
      721 ctcgtatcca taaactgaaa aagtttctcc tgacattcat tgctcaatgc atgaatttca
      781 tctaaaaata agactccatt attagctaat tctgctaaac cctttcttcc atcttcggct
      841 cctgtatatg caccttttac atgcccaaaa aaattggaca agaaaagttc tggattattg
      901 gcatattcac tacaattaac tgaaatgaaa gatgccttct cttctatata attattggcc
      961 aacaaatcct tatacacata atttgctaaa tagctttttc cagttccagt ctcgccatga
     1021 agtaaaatgg gtaagccatt tggcggatag tagattgctg ctttaatttg agcaattaca
     1081 tttcgcagac ttccttgact tccgataacc ctttctattc caaccctaaa agagctatca
     1141 taccattttt ccgcttcctc tgaagagcta aaaatttgtt gatttggaac tttctccggt
     1201 aaagaaaaga agattactgg acgagtattg atttttccaa attctccatc ttttacgaat
     1261 tcattcaaat attgagagac aatattacga ctaacattta atttttcaga tattttctgt
     1321 gctgtattgt tcccccattt atttttaatc tcagagttct ctatgacata attgacaatg
     1381 cgttctttta attcttccat cacagcacct cctcgacact attcgaattt tgccgacagt
     1441 atttttccat aaatatgact tgctaaaaag gctttgttta gatcattcaa ttggaacgtt
     1501 gcttgttttg cttgatccgc taagacgttg aaaagatact ctggcgaaat cgcatacccc
     1561 aaaatatcaa cgattgttgc aatcagatct tcttttgatt cttcatctga aagtaattca
     1621 cgatacaaca tcgtttgttt gcttactgtg tcatattcct ctaaggattc atctgctaaa
     1681 agaaccactt gttccaacaa acggtcggat aaatatttat agaaaatcag ccccaataaa
     1741 taatttttat attctgacgc atccatttta ctacggagat tatctgccgc actaaataac
     1801 ttctgattta attctgcacc caaaaagata cactcctatc tcttatttaa agttgctata
     1861 taccctctta ttttagcata ctcagtttta gaaaaagcaa aaaaataaag aaacttattc
     1921 ttgataacaa gctacgtgac gtatatccct aacacaaaaa tagccatatc ctttggtttg
     1981 gatacggcta tttgatatta ctcattcaat tatcaaatca cagtaggtgt gaatctcatc
     2041 gggatcggca atatctactt ctgattagga tcaaaggtga ttttctcaat aaaggtgtga
     2101 tacaaagctt taacttgctt cttctcactc tggataagaa agcaatccaa actagtcaat
     2161 atctgttgga catctttgac ttgaatcggt tccccttgtt gttgaaagat ccctaagatt
     2221 tcattctctc gaatttgtaa ctgtcttcgt ttttcagtca attcatttaa acgatctttt
     2281 aacatcggaa acaattcggg actatcttcc aaagcagctt cctgtttttg ctctaatagt
     2341 tagattttgc gtaccatttt cttcttttat tttccttcga ttgctgattt taatatagtc
     2401 taaaggaaat ttatatttat tgtattcttt ataacttcaa gagaacttcg tttgagtatt
     2461 actgtatttt tttaattcca taagaaaatt agttattacc tgccacctaa tcagcaaatc
     2521 tgaacctgag tcaatataat taatattatt ttccatctcc aacattgtaa gtactgttgg
     2581 tgttaatata gggactttgt acaattgtat ttttttgatt ttatcatctg cacaaaggag
     2641 attttttaaa tctaatcctt cttttatttc aaaagttgaa ttttttaaat ctataagagt
     2701 aaaaccaatt ccttcatact gaatttctga aaatcgaatt tttctcccag ttgatagatc
     2761 aaggggcaga aaacctttta ttacattgtg agtgtcacca tcatttacaa tgtcattagg
     2821 aattttgatt ttagaattct gtggagcacc tattggaaga taataattca ttataatttt
     2881 gtagtcttca tgaggaaata aagtactttt aggattgcta agatattctc gtaccgaatc
     2941 taagaatggc ttatccattg aatcttcatc cccatttaaa ctaaaaaaca ttcctacaat
     3001 tgcttttgca actctaagtg aattcatttt ctttattgaa tgtgttaaat cagacttacc
     3061 attctttaaa cttgtattgt tataaaggaa atttttattg tcttctgcaa atctaatata
     3121 ttcctcaaca tactcactac caaaaaaact attacattta ctacataacg
//
LOCUS       DAZBSA010000175         3162 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000175 DAZBSA010000000
VERSION     DAZBSA010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.181"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(63..2555)
                     /locus_tag="ACHGMT_002774"
     CDS             complement(63..2555)
                     /locus_tag="ACHGMT_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFN6836460.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(2562..3029)
                     /locus_tag="ACHGMT_002775"
     CDS             complement(2562..3029)
                     /locus_tag="ACHGMT_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFN6836461.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT          838 a          697 c          518 g         1109 t
ORIGIN      
        1 cttgtcacaa cctcttaatc aagtcttact attataaaaa ttcagctaca caggccaagt
       61 aactataggg aacctactgt ttccttttta ggtccgcgaa cattcaatgt gatcttacct
      121 tttgaagcac ctactgttac gtggtcacct aaacgaattt ctccgccaag caaagcctcg
      181 ctcaatcgat cttcaatttc tttttgaagt gcgcgacgga ttggacgtgc accatattca
      241 ggatcaaatc cagctttccc gatcacatcg attgcagatg gtgtgatttt cacttgaata
      301 tcttgttctt tcaatcgttt gataatcgct ttgctcatga tttggacgat tgtatggatt
      361 tcatctttac tcaatgaatg gaagacgaca gtctcatcga ttcgattcaa gaattcaggt
      421 cggaaagctt tctttaactc ttctaagata cgtttttgca tggctttgtg atctttagta
      481 acatcttgga cattgaatcc gacatttttt tcttctcgaa tttgtgtcgc tccgatattt
      541 gaagtcataa tcatgattgt atttctaaag tccacttttc tacctttaga atctgttaaa
      601 tgaccatcat ccaatacttg cagaagtaaa ttgaaaacgt ctggatgtgc tttctccact
      661 tcatctaaga gaatcactga atatggtttt tgacgaattt tttctgtcaa ctgtccacct
      721 tcatcatacc ctacgtatcc tggaggagac ccgattaatc ggcttgtact gtatttttcc
      781 ataaattctg acatatcaac acggatcagg gcatcttcac ttccaaacat gacttctgaa
      841 agtgctttgg ccaattctgt ttttccgaca ccagttggtc caaggaacat gaatgaacca
      901 attggacgat caggatcttt caatccgctt cgtgcacggc ggatcgcacg tgaaactgct
      961 ttgacagcct catcttgacc aacgacacgt tcatgcagaa gtccttccag ttctaacaag
     1021 cgttcgcttt cttttttctc caactgctgt agagggacac cagtccactc cgatacaact
     1081 gtagcaacat cttcttctgt tacacgatca gaataacctt gctcctgttt tgcttcaatt
     1141 gcagaaactt tttgcagctt tttacgtagt gctttttctt gtctgcgtaa ttgagcagca
     1201 ttttcaaagt cttgtttttg gatcgctgct tccttttctt gtactagttc atcgatttct
     1261 agtttgatca cagttgattt tgttaagtga tcggtttgat ccaaacggac tttcgctgct
     1321 gattcatcga tcaaatcaat tgctttatcg ggtagttgac gagaagtgat ataacgaaca
     1381 gaaagattga ctgctgctcg tacagcttca tctgtgatct ccactccatg gtgctcttca
     1441 tatcttgtac gtaatccttg aagaatcact tctgcttctt caggagttgg ctcatctact
     1501 tgaatgcgag caaaacgtcg ctctaatgca gaatcttttt cgatatattt ttgatattca
     1561 tctagggtcg ttgctccgat cgtttgtaat tctcctcgag ctaacgccgg tttcaagata
     1621 ttcgaagcat caattgctcc ttctgctcca cctgcaccaa ttaaggtatg caattcatca
     1681 atgaataaga ttacttgacc atcattatag atttcgtcga tgaccttttt cattcgatct
     1741 tcaaattcac cgcgatattt cgtaccagca accaaagatc ccatatcaag catcatcagg
     1801 cgcttttctt gcatgtcttc tggcacttca cctaaaatga ttttttgagc taacccttca
     1861 gcaatggctg ttttgcctac accaggttca cccaccagta cagggttatt ctttgtccgt
     1921 cggctaagaa tctggataag ccgtttcact tctctcgaac gaccaacaac tgggtccagc
     1981 ttgttttctt ttgctaattt cgtcaaatca cgtgctaatg aatctagcgt aggtgttcct
     2041 tgcggtgttt gccgtttatc ttgtacattt acatttctac gacgattcat tccttttcca
     2101 gcagaatcac cagctagccc cattttttgt ttcagaactt gacgggcttt tgaaaggctg
     2161 atacctaagt tgatcatgat tcgagtagcc atgatacttt catcttttaa taagctcatc
     2221 aaaatatgtt ctgttccaac tttttgagaa ccagttttct tcacttcgct gctagctaaa
     2281 gcaaaaactt gtttcattct tggtgaatat ggaagaaaga ctcctttagg gtaagaacgg
     2341 ataccaccat aaccaatcag atgttcaatt tcttcataaa tttccgtttc attcaaatcc
     2401 atttcacgta atacttttcc agcaattcca ttttgctcga ccactaaggc tagtaaaaca
     2461 tgttctgaac taactgattg atgtttcaac cgttttgctt cttcttgtgc aatttctaat
     2521 actgcttggg ctctttcagt aaaaagttct tccatctaat ctcacttccc atcttcataa
     2581 cttaaacgtt ccagaaatgc tcggagaatc cgagcacgtg tatgatcttc atattcattg
     2641 aaattcaatg tgttttttgc aattgcactc aacatcaagt ttccttcttt ttttgtaatt
     2701 atttggtctt cataaagttt ttgaataata gcaaaagcat ttttctcact gattgtatca
     2761 tcaaataact gatttatctg ctcaagtaat tgttttttat ctgaaatcct tacttttgca
     2821 attcgtatat aaccaccacc accacgtttg ctttctacta gataaccacg ttggatggta
     2881 aaacgggtat taatcacata gttgatttgt gaaggaacac agttaaagag gtctgccatt
     2941 tcaatccttc gaatctcgat catttcacta ttttccagaa ttttctttat atatgattca
     3001 attaaatccg aggtattctg atgtgccatc ttattcattc cctttgacta atattgacct
     3061 taattataac gaatttgata atagaaagaa agaagaacgc tttttgttaa ataaatcctt
     3121 tagaatcaac aaaaaaaaat acggaatata ctattccgta tc
//
LOCUS       DAZBSA010000176         3086 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000176 DAZBSA010000000
VERSION     DAZBSA010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.182"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..72)
                     /locus_tag="ACHGMT_002776"
     CDS             complement(<1..72)
                     /locus_tag="ACHGMT_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFN6836462.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGK"
     gene            complement(202..786)
                     /locus_tag="ACHGMT_002777"
     CDS             complement(202..786)
                     /locus_tag="ACHGMT_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN6836463.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(1117..1350)
                     /locus_tag="ACHGMT_002778"
     CDS             complement(1117..1350)
                     /locus_tag="ACHGMT_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFN6836464.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(1365..2315)
                     /locus_tag="ACHGMT_002779"
     CDS             complement(1365..2315)
                     /locus_tag="ACHGMT_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFN6836465.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(2312..>3086)
                     /locus_tag="ACHGMT_002780"
     CDS             complement(2312..>3086)
                     /locus_tag="ACHGMT_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFN6836466.1"
                     /translation="VIGIVSGTVGCFIILRGMSLMGDAISHAVLPGVAISYILGINFF
                     IGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSSFLALGVILIGVANSSTDLFHILF
                     GNILAVQDIDKWITIGVSVLVLVIVVLFFKELLITSFDPLMAKAIGMNVSFYHYLLMV
                     LLTLVAVTAMQSVGTILIVAMLITPAATAYLYANSLKTMIFISATVGASSSLLGLFIG
                     YSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKKERSL"
BASE COUNT         1037 a          565 c          383 g         1101 t
ORIGIN      
        1 ctttcctaaa aaaataatga ccggaaaaaa aatatttaaa gtaatcaagg ttaacggatt
       61 gatttttgac actctttcac ctccaaaaaa taagatcgca ctgttatctt aagctgataa
      121 aaatgcaccg ttattcagat aacagtgcga tcttatcaaa aagcataact gagaacaatt
      181 ctcaatgcaa gcttttttta attaactcag taattcaatc cacccaggaa cagaaaattg
      241 atacaatctt ctggttaata gcatagtctt ttcttttgaa tagctatgct tcaatatatc
      301 atagaatgta gcgtagaatg agtaaatgta gaagtggatt tcttcttcat taaagactaa
      361 attttgattg atcattccga tagtctttaa cttatctaga caacagactg tctttttcgt
      421 cacaaatgat gtaatttttt caacaatatc ttgatatttg gttccagctg attggaataa
      481 aatcaaatcg aatatacgtt tattctcaaa aatataatta ataaatggat ctaattcatt
      541 gaagttctct ctccaacttt tctctatatc tatattttct aagtttaaga ggtatttggt
      601 aaactcttca ttatacatat taataagcgc attaatatgc ggctgaataa tttcttcaaa
      661 taacgcttcc ttagaagtaa aatgtttata aaaagcaccc gtagttacgc ctgcggtcag
      721 gcacagttgc cttatcttag tttgctgaaa tccttctttt tcaaaatggc ttagtccact
      781 ttctattaac ttttggtgag ttataatata atctctggtt tctatgggta cttcctccta
      841 ataagatgaa ttaactttat tatcttacac aggacttgaa gtttcaatcg ttaacgttta
      901 ttactgaact cattccttag agctaaacta atgagaaaga gcagaataac tgatacaaaa
      961 agcaatccta caagaattaa tagactcatc taaatcattc accttttcta aaataatccg
     1021 agaaagattt aaaaaaatta tatccagcta ttaggataag tcctatcata atcactccaa
     1081 tagccatttt cttatcctcc ctaaaaataa agtcttttac ttaaaacctt ttttgtaatc
     1141 gtcaataaat tttttccctt tatcagttaa ttgaaatcct atatctaaat agtcctcata
     1201 tctaagttta gccagcatct cacttacaga tgctggacta acacttagaa cttttgctaa
     1261 atcattattt tttaatttct tttttctttt tgataattcc tgaattgcta gtaaataaat
     1321 tagtatagta gtcgttttaa tttgtaccat acctaccctc caaattattt cgaaaggcct
     1381 tcagcaattt tatccaggtt ccatttcatc atcgcatagt aactatcacc atcttgtcct
     1441 ttttctgcaa ttgaatcagt aaaaatcgtt gaatagatag gaatattggt atcttttgat
     1501 actgttttca ttggtctatc atccacactg ctttctacga ataaggaggg aacttttgtt
     1561 gtgcgtaatt tttcaactaa gtgttttatt tgatctggtg ttccttcttc ttcagtgttg
     1621 atttcccaaa tgtaagcaga aggcacatta tacgctttcg agaaatattt aaagcatcct
     1681 tcacttgtaa caatcatttt tttatcattc ggaattgaag caaatttaga tttagcttct
     1741 ttgtctagag aatccaattt ttcaatatac ttatctagat tttctttata gaatttttta
     1801 ttatcaggat ctttttccgc taactgtttt tcaatatttt tagcgtaaat aataccgttt
     1861 tctaaattta accaagcatg aggatcttcc ttccctttct cactctgacc ctctaagtaa
     1921 ataacatcta tgccatcact tgctgcaaaa tagtctttgt tttcctcttt gttcgcattt
     1981 ttaactaatt ttgtaaacca agcatttcct ccagtttcca agttaacacc gttataaaaa
     2041 atcaaatctg cttttgaagt tttttgaaca tcttcaggca aaggttcata ttcgtgggga
     2101 tctttcccaa taggtacgat actgtgtaaa tcaattttat cttttgctat attttcggta
     2161 atatctgcta aaatcgaatt agtaactact acttttaatt tttcgttcga agccacttta
     2221 tctttgtctt tactatttat tgaacatgcc ccgaatataa aaagagcaat caaaatcccc
     2281 agacttccca gtactgcttt ccattttctc atcataacga tctctccttc tttcttaaaa
     2341 atttttgttt tggtgagatt agaaaactaa ttacaaataa gaatgccgct gttaaaacaa
     2401 tacttgatcc tgctgctatg ttaaaactat aaccaataaa caatccaagc aatgatgaac
     2461 ttgctccgac tgttgcagaa ataaatatca tagttttcaa gctatttgca tataaatatg
     2521 ctgtagctgc tggtgtaatt aacattgcaa caattaatat agttcccaca ctttgcatag
     2581 ctgttactgc tactaaagtt aatagaacca tcaatagata atgataaaaa ctgacattca
     2641 tccctattgc ttttgccatt aatggatcaa aagaagtgat taataattct ttaaaaaata
     2701 gaactactat aaccaacact aaaaccgaaa cgccaattgt aatccactta tcaatatctt
     2761 gtacagctaa aatatttcca aataaaatat gaaataaatc cgttgagcta tttgcaactc
     2821 ctataaggat aactcctagt gctaaaaaag aactgaaagt aataccaatt gctgtatctc
     2881 cttttattac actattgctt tttataaaag taataatgat agaagctaat agaccaaata
     2941 caatcgcacc aataaaaaaa ttaataccta aaatataaga aattgctact cctggcaaaa
     3001 cagcatgaga aatagcatca cccataagag acattcctct taaaataata aaacaaccta
     3061 ctgttccaga aacaatccct ataact
//
LOCUS       DAZBSA010000177         3012 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000177 DAZBSA010000000
VERSION     DAZBSA010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.183"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(101..708)
                     /locus_tag="ACHGMT_002781"
                     /pseudo
     CDS             complement(101..708)
                     /locus_tag="ACHGMT_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            1231..1905
                     /locus_tag="ACHGMT_002782"
                     /pseudo
     CDS             1231..1905
                     /locus_tag="ACHGMT_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            1898..2059
                     /locus_tag="ACHGMT_002783"
     CDS             1898..2059
                     /locus_tag="ACHGMT_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836467.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
     gene            2543..>3012
                     /locus_tag="ACHGMT_002784"
     CDS             2543..>3012
                     /locus_tag="ACHGMT_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836468.1"
                     /translation="MNPTLITKKELLKKLDISTGVLANLIRNGMPKEGEMFNLDKIIT
                     WRENWSKNILGELEVGRVYTNKEISEKFKCSKQGGMRRSHQTNTLVLFSDQTGSNVYK
                     DKWLNGILQYTGMGLKGDQVLDKNQNKVLANSKSNFVKIHLFETFKPKEHTYLG"
BASE COUNT         1103 a          427 c          509 g          973 t
ORIGIN      
        1 tatgaatata aatagagtgt atcttctttg ataaatgtca gaaaaagtgt gacaaataac
       61 catcagtaaa attacttgtc atactattga taaggagtat ttaattgaag agaatgatta
      121 ggagaaagga tctccgtact ttttatagta atcatagaat ccaagtttgt atagacgaat
      181 ctttttttct cttatttctt ctgtttcaaa atggactgct tgaagaatca agtctgtgaa
      241 ttctatagga gctgttccat tcgcagtaat caaattacga tctttcacag cttgttgttc
      301 tataaaatct tctttattct gataattttc cgattctaac caaggaaatt gcgcatttcc
      361 ggtgtgtttg taaccttgca ataatccgct ctgagctaaa taatcaactg ccccacaaat
      421 agcagctact ggtacgtctg tttttaacaa tttatcgatt atttgatata aatgatgatt
      481 ttttatgtcc catgaattgc caccgatcat aataaaaagg tcaacttttt caggtatttg
      541 ttcgattaag taatcgattt ttgtttttaa tcctccaatg gaagtgacct ctgattggat
      601 agaagcagtt ttgttttgcc atccctcttt ttggtttagt gtgctagtaa tataagcacc
      661 ttcccaatcg gcatattcct ctaatagaaa cgtaatcgca gttttcattt tcaagctctc
      721 ctttactatc tatgacaagg acaacagttt taacatacag acattcatta aatttggata
      781 gcgacagcga aaacaacaat tcttaagttt tagacagagt atacagacag tcaacttaat
      841 taaatacgaa ctaaataaaa atgacaggta atcatttttg tttaccagca ttataacatt
      901 ttgcatacat ggatgacaaa agaaccaatt cccacttgct tgagtacaat cttaaaagaa
      961 actggaaggc aaaaagtaat tatagaaacg aaaaataaat catatcgggc actcagacat
     1021 gaaaacaacc ctagaaatat ataactagtc caatgaaaaa aaagcgaatg ccgtagaaaa
     1081 caaggattct gcgtacattc atatctcatt ttgcgagcat atctgctatt actgtgacta
     1141 aatgttaata tggttaaaat gaccgttata ttttttatat aaatatcgta taattagtat
     1201 gaatatagaa gaaggaaaga aggtgcaatc ttggaaccta aagacaagta tttagattac
     1261 gctagggtca ataaagataa atttattgaa acaatcatca agggaaaaat ccctgatgat
     1321 gaaaaagatg cggtattcat ggctggaagc ccaggagcag gtaaaacaga ggttgcacta
     1381 ggtttagctt aaaattatga caaccatgtt gtgattgatg cggactattt tagagcacaa
     1441 tttccagaat acaatggaaa gaactcaagt gtttttcaga aagcttcttc ttggttagtt
     1501 gaacaatcat taaaatatgt actggaacat ggctattctt tcatactaga ggatactttt
     1561 gctatcctta gtgcagagaa aaatattatt cgagccctta aaaataactt tcgcgttacg
     1621 attttctatg tttaccaaga tcccaaagtt gcctgggatt tcactcgaaa acgtgaacta
     1681 gctgaaggac gtcatgtgcc aaaagaaatg tttatcaatg cgttttttaa gtccagagag
     1741 aatattgaaa aagtcaaaaa tagacatcct gaagtagtcc ttcacataat gattaaggat
     1801 tatcaaaacg acatttctga agtgcattat gcagcagata atattcaatt agtactacca
     1861 atacaatata cttcaaaaga gttggaggag gaattacatg actgagaaag aacaagtaca
     1921 acaaatcgtg aagaagtata ataaaagtat cgctgattta tcagaaaacg cctcagcgaa
     1981 agaatttaaa acagtaatga attacgtagc agatgaagca aatcgtaagc agcgaaaact
     2041 agttgggttg gataaataaa ttttttaaac catatccttg taaagttgag ttttttattt
     2101 taatgaatta agactattaa atagataatt attgtaactt aaaaaacaca gctaaaaatt
     2161 agttgtgttt tttattaggt ttacttttct ggtataaaag gaactagcta ctttttcaaa
     2221 atttctaaat cattcaatat gtagcgctta ctaaattatt cgccataaaa ttttataatt
     2281 ggatcactca ttgttttacc taagtcatat ttaagtaata accggtgttt ttacataaga
     2341 agacttcggg tattttttag tttgattagc tctgatatac ctataaagaa tatgctaagg
     2401 taagaatagt aaaaattcac atagatgagg tataataatg atatttttat atattacaga
     2461 taacttgaca caaaatgagt ttttttcgat ttttcaagtt atctaatgca attatattga
     2521 tatcattagg agctgcattg ttatgaatcc aacattgatt actaagaaag aattactaaa
     2581 aaaattagat atttctactg gtgtattagc taatttaatt agaaatggca tgccaaaaga
     2641 gggagaaatg tttaatcttg ataagattat tacatggcga gaaaattggt ctaaaaacat
     2701 attaggtgag ttagaagttg gtagagtata tactaacaaa gaaatttcag agaagtttaa
     2761 atgttccaaa cagggtggaa tgagaagatc tcatcaaact aatactcttg tactgtttag
     2821 tgatcaaaca ggaagtaatg tttataaaga taagtggtta aatggtattt tacaatatac
     2881 cggtatgggg ctaaaagggg atcaagtatt ggataagaat caaaataaag ttttagccaa
     2941 ttcaaaaagt aattttgtaa agattcattt atttgaaaca tttaaaccaa aagaacacac
     3001 atatctggga ga
//
LOCUS       DAZBSA010000178         2932 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000178 DAZBSA010000000
VERSION     DAZBSA010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.184"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1412
                     /locus_tag="ACHGMT_002785"
     CDS             <1..1412
                     /locus_tag="ACHGMT_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFN6836469.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNA
                     DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTY
                     PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYT
                     WKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTG
                     KTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNV
                     VVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGL
                     ILLGTLGLLGYKNRKKENE"
     gene            1546..2889
                     /locus_tag="ACHGMT_002786"
     CDS             1546..2889
                     /locus_tag="ACHGMT_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN6836470.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
BASE COUNT         1154 a          401 c          569 g          808 t
ORIGIN      
        1 aaccaacagt agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg
       61 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      121 caattgatac caacacacca ggtaattacg aaggtgtcat tgaagtaacg tatccagacg
      181 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct gacgccgata
      241 aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg aggcacagta gatttaacag
      301 ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg
      361 gcggtacaat tgataccaac acaccaggca attatgaagg tgtcattgaa gtaacgtatc
      421 cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctaatg
      481 cagataaata caatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg
      541 catctgaggg aattaagaac aaagaagatt taccaaaagg aactacttat gcttggaaag
      601 agaaagtaga tgttagcaca gctggaaata aaaaaggtac tattgtagtg acatatccag
      661 atggatcatc tgatgaagtt gaagtagacg ttacagtgac agatactcgt tccgatgcag
      721 ataaatataa tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat
      781 ctgagggaat taagaacaaa gaagatctac caaaaggaac tacttatact tggaaagaaa
      841 aagtagatgt tagtacagct ggaaacaaaa aaggtactgt agtagtaaca tatccagatg
      901 gatctaaaga agaagtagaa gttactattt ctgtagtaga taaaaaagca ccgaataaac
      961 ctcaagttga tcctattaca gagggtgacc aaattgttac tggtaaaact gaaccaaatg
     1021 cagaggtgac agttacatta ccagacggaa gtcaacacca tgggacagct gataaaaatg
     1081 gtaattttac agttaaagtt cctaaattag aggcagggac aaaagtgata gtaactgcaa
     1141 ctgatgaatc tggtaatact agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag
     1201 acagtggaaa aaacggtagt aaaggtagta agactgacaa tcaaggtagc aacagcaatc
     1261 aagacaaaaa tagaggcaaa agccagtcct caaaagtttt tcctaagacg ggagagagtg
     1321 acagtaatat ctttactatt agcggtggtt taatactgtt aggaacgtta ggattattag
     1381 ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt cttctctgag
     1441 aagaggaaga atctttttct tcctcttttt gcttttttat gatgggaaat aagtgttagt
     1501 taaagatgaa tatacttttt atagaaagga aaagaatgta attaagtgga aaaaatagta
     1561 attattggtt caaaccattc agggctagcc gcagctcgat ttctaaactt aagcgattca
     1621 aattatgaag ttattctaat cgataaaaaa tcagaaatta gttacttgga atgtgggaca
     1681 tcgttattta taaaaggaga agtaagtaat ttagaagatc ttttttatca aaatagaatg
     1741 acactaaatt ctgagataaa aaagttattt ctagagacag aagttgacaa aattgatatt
     1801 caaaataaaa agattagttg tgtaaaaaaa gatggtacaa gattctctca agaatatgac
     1861 aaagttatat tagctacagg ttcaaagcaa attatgctag atataccggg gaataattta
     1921 gctaatattt tttcgataaa gaattttcca aatgcaaaag agatttttaa agctttggat
     1981 gatagtacta ttaaaaaagt aggaattatt ggagcaggat atattggagt tgaaattgct
     2041 gaagccataa gaaaaagagg gaaagaagta tatcttttcg atgttgcaga tagagtgttg
     2101 tcgacttatt acgaccgtag tttttcagat aaagttgaag aaatactatc aaaaaatgga
     2161 atacatcttt gtctatcaga aagtcctaaa aaatatttag gaagaaaaaa gattgaaaaa
     2221 atagtcacta caagtggtac ccaatactct atggatatgg tcgtacaagc tataggattt
     2281 ctatctaata gtccaattgg ggaaaaggaa ctcttgagag atgttagtgg agcatatcta
     2341 actaatgaat accagcaaac aaatataaaa gatatatatg ctattgggga ttgtgctaca
     2401 acttttttta cgtccattaa tagaagtagt gttgattttt ctatttctaa tgctctaaga
     2461 acagcttata ttgcaactca acatattaac aatcaagatt ttacaccttc tggttcccaa
     2521 tttacaaatg gcttttctat atttaattac aatttttacg cagcaggttt gaatttaaaa
     2581 acagcacgat tattaagtga gtctatagat tatatagaaa tagaagattt cattagacct
     2641 gcttttttga gaaaaaatag tcgtgtaaaa ttaagaatta gctttgaaaa aaatacaaag
     2701 agaatagtag gtgtacaact ttgtagtcaa gaagatttat ctggactatt atcaatgttt
     2761 tctcttgcta tagaagaaca aattacatta gacaaattga aaatgttaga ttatttattt
     2821 catcctagtt ttagccaacc ctataatttt attttaaagg cagtaacagg attaaacatt
     2881 gaaacataat cgttactaaa tgaaatcaag aaatatactg tattactcaa tt
//
LOCUS       DAZBSA010000179         2918 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000179 DAZBSA010000000
VERSION     DAZBSA010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.185"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..218)
                     /locus_tag="ACHGMT_002787"
     CDS             complement(<1..218)
                     /locus_tag="ACHGMT_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6836471.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDT"
     gene            450..1184
                     /locus_tag="ACHGMT_002788"
     CDS             450..1184
                     /locus_tag="ACHGMT_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN6836472.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            1223..2365
                     /locus_tag="ACHGMT_002789"
     CDS             1223..2365
                     /locus_tag="ACHGMT_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6836473.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         1102 a          457 c          490 g          869 t
ORIGIN      
        1 gtatcaattt ttataacagg taacccgtat tgagaaacat attttctaat cgttcctact
       61 gatacaccta gatattcact tgcctgctga tagtccatat actctagagt gactccagaa
      121 ataatgtctt ctagcatagg ctttagcact gtaattatct ctttcacttg ttgttcaact
      181 tgtatagaaa caatgctatc aatgatttgt agagacatat ttccccctcc tttcgtgatt
      241 gcttgaacaa attttctaag aattatcttc gtattttttg ctcaaaataa aagtattttg
      301 ttattaaccg attaatcaaa caaaaaatga ttaccagttc atcgacatac acataatacc
      361 tcgataaaaa aggggtgtca atagttttgt aaaatttatt tacaacaccc cttttttgag
      421 gtatactaat tttaggaggt gtggtcaata tgataaaacg tccaccaata aattatttag
      481 aacgaaaaaa gatactcgga acaaaaataa aagctataag aaagagtaaa aaattaacac
      541 aaccagcatt tggtcttatg attaacaacg gtcaattaat tgacaaaaag actatctacg
      601 aatgggagaa gggaacctat ttaccaatac cagagcgatt atcccgaatt gctgatttgg
      661 gaaatatgag tatagaggag ttagtttgtg gcaatgtaga agaatatatt ttgggaatta
      721 ttctttatag agatagcatt gtactagatg gtataacatt tccagataaa aacctgttcc
      781 agcatcttcg tcaacagttc ccaccagtac actctaattt agatacatgg ctggatagat
      841 attccaaatt agaaccagag atgcaagaat ttattgctaa caagacttgt aataaagtta
      901 aaaatgaaaa aattagtctt ttcaatatct taaaaataga agaattattc atcaatgcta
      961 ttgtagaaga atttgacaat aacattcttt tcctcactag cagtatagaa gaactactag
     1021 aaagaatggt agatgaatgg ctacctattc aattgaaaga catgtcttat cccgaagaag
     1081 ctgtgagaga aattactgat aatataaaca agctagaaca aacgatttca tcaattggga
     1141 aaaaatacac taagaaaaaa atgaagggag gtgataccat ctagtggtaa gtactccttg
     1201 taaactaaga ggagatgatt tcatggcaaa aatatcaaag tacaaaaaga aaaacggtca
     1261 agtggcgtgg atgtataaag gacacattgc caccgatcca cgaactggtg aaaaaataaa
     1321 tactacgaga agaggtttta gtagcaaaca agaagctcaa aaagattatg acgagtacag
     1381 acatcaaata ttatacggtg ttaaaaagaa agcacctgat atgacatttg aagacctgta
     1441 taatgaatgg ataactcatc agcgtaccag tgttaaagct tcaacaatcg caatctcagt
     1501 aagatacgca aataatcaaa tacttccagc atttggtaag cttaaactat cgaatatctc
     1561 tgtcccatat tgtcaaaaag tggtagatga atggcacagt aaatatgaaa gttacgatta
     1621 tatgagaaaa cagactgcac aaattttacg ctacggggtt gcaatgcaat atattgacaa
     1681 taatcctatg gaaaaaacac ttcttccacg aaaaaaagag tatgagaaaa atcgtaaatt
     1741 ttactcaaaa gaggagctta ataatttact ggatgctttt aaagattttg gtaacatgaa
     1801 acaatacgcc ttcttcagat tattggcata cacaggtatg agaaagagtg aagtactcgc
     1861 tttacagtgg aaagacatag acacgtttaa taaagaacta catgtcaata aaactcttgc
     1921 tgtagatgaa tttggtaaag ttataattca atctccgaaa acaagagctt ctagacgagt
     1981 tatctcttta gatactgaga ctctttctat cctcaacaac tggaaattac agcaaaaaga
     2041 agagtattta aaacttggat acaatactag ctctaaagag caacatgttt ttacaacagt
     2101 taaaaacact ctatacattc caaacactgt taatgattgg ttaagatata ttttgaaaaa
     2161 atataacttg cctcgtatta cacctcatgg gtttagacat acacatgcta gtttattatt
     2221 agaagcagga gaatctgtca aggtagttca acaacgatta gggcatgaaa atagtaaagt
     2281 gacattagac atctatgcac atattacaaa taatgcacct aagaaaacag gtcaagactt
     2341 tgctgatatg atggctcatc agtaaaagag ctggtcaaaa tctggtcatt tctagtatac
     2401 aggcaaaaca aaaaagctag aacccttata tatcaagggt tctagctcct ataacaggtt
     2461 tacccaacac tgccttccaa tgactctttt gggaatcaga ggaaagaatc aggctttata
     2521 gagatatttc atctaacaaa ttactttaga ctgacagtat atatcagcag gtactgtcat
     2581 cgggaacgta gccaatatcg tatccaaaac agcaggtaga gaagctattt gaggattcaa
     2641 gttattctat ttcaaccatg aagttaagtt cattaaaaat aaagatagtt ggtatttcaa
     2701 taatttttgt atagagttaa tcactttatt gatgctaatt catcttgatt ggtattataa
     2761 tcgtattcaa gttgcgtggc gtgcttttgc tagaaaatat ataactgttc tttataaaga
     2821 aatataagga ttaaacattt tttatcatag agttttactt atctatagaa ttatgtctgt
     2881 tcctaattca gtttgatgaa ttaactgata aaaaaata
//
LOCUS       DAZBSA010000180         2861 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000180 DAZBSA010000000
VERSION     DAZBSA010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.186"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1087
                     /locus_tag="ACHGMT_002790"
     CDS             <1..1087
                     /locus_tag="ACHGMT_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6836474.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..2447)
                     /locus_tag="ACHGMT_002791"
     CDS             complement(1305..2447)
                     /locus_tag="ACHGMT_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6836475.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2452..2637)
                     /locus_tag="ACHGMT_002792"
     CDS             complement(2452..2637)
                     /locus_tag="ACHGMT_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFN6836476.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
BASE COUNT          949 a          526 c          448 g          938 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct atgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggacgc
     1921 tgagttttta ttttacctat agtatctaca ggattttctt caataatttc aagtacaatt
     1981 gcccttttaa aaactactct caacttattg aatatctgtt taatataatt tggtgataat
     2041 ggttgctcaa tgcccttttt tacaacatgg attttcgata aaaactgttg aaattcctgt
     2101 atatgaattg gtcgaatctt tacaacctgc atttctccaa aaaaatccaa tgcacgttgt
     2161 aaaatatgat ctgttttcat atatgttttt tctacagttc ccaacttata ccaaggaaaa
     2221 aaatactctc gataaaaatc atgaaacgtt attccttgca gataaattcc ttgtgaatca
     2281 gaaaaacttt gttttaactc ttcgagtccc cttctagctt ctccttgagt tttgaagcct
     2341 cttctggtca cttgaattct ttttccaaag cgatcttgtc ctaaagttgc agaaaaaaac
     2401 caagtgcctc tttttctgtc tttataaaca ttcgcaattt tcctcatttt actattcctc
     2461 ccaagaaata tggagaactt cttctaaaat tgaaatagga attcttttta tttttgaatt
     2521 acgataaaaa aagtatcctt tctcaactaa aagttcttta cattccttga agattttatt
     2581 agcatagtac tgagaaaaac ctagattaat caaatctttt cgagtagctg ttttcattat
     2641 agcaccttcc ttactttttt tcatttattt ctatcagctg cttttgtcaa aagcacatag
     2701 gaatttcacc tctccccaag tttacggcga gccttacgga agtatcatta tcccaacttt
     2761 aaatcatcgc tttttattga ctataaaaat tattttagaa tatttatcgc tccttcttta
     2821 cagcaagaaa tcatggcgca actaaaatat gctccttaaa g
//
LOCUS       DAZBSA010000181         2659 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000181 DAZBSA010000000
VERSION     DAZBSA010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.187"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..254
                     /locus_tag="ACHGMT_002793"
     CDS             <1..254
                     /locus_tag="ACHGMT_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836477.1"
                     /translation="VQDEKISNFELSTPSSKIYDDAYRFECQDLEALLSANNLNNIEH
                     ENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            380..637
                     /locus_tag="ACHGMT_002794"
     CDS             380..637
                     /locus_tag="ACHGMT_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836478.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(889..1461)
                     /locus_tag="ACHGMT_002795"
     CDS             complement(889..1461)
                     /locus_tag="ACHGMT_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN6836479.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1477..2082)
                     /gene="fic"
                     /locus_tag="ACHGMT_002796"
     CDS             complement(1477..2082)
                     /gene="fic"
                     /locus_tag="ACHGMT_002796"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFN6836480.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            complement(2483..>2659)
                     /locus_tag="ACHGMT_002797"
     CDS             complement(2483..>2659)
                     /locus_tag="ACHGMT_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6836481.1"
                     /translation="YLLGMALAYSVGSLIKVNVSLDLFTILLAVGVSSVIGLVFSVMP
                     ASEAAKKDLIDILR"
BASE COUNT          846 a          512 c          393 g          908 t
ORIGIN      
        1 tggtccaaga tgagaaaata agtaatttcg aattaagcac tccttctagt aagatttatg
       61 acgatgcata tagatttgaa tgtcaagatc tagaagccct cctttccgca aataatctaa
      121 acaatataga acatgaaaac tttgttgtaa acactagatt taatttagtt tctgaaaagg
      181 actcagattt gttagatttg ctgatagagc ttactaaaaa tctagataag aaagaaaaca
      241 ttactgtact ataactagca tcggtccatc attaagtttg gactattaga acacttgcta
      301 ttacagaaaa aggtatgcta gccacgctta ctatttattg gtatcaaaca agtatcttaa
      361 tttgaaagga agatgattta tggctaattt atatatagag aacaaaggag acttgaatgc
      421 cattattact ttaatcgtag ttatggtaaa atcaataggt aatatcacaa taggaatgct
      481 gtttattata ttagcagcag tgctgatttt tatgatagtt atttggttct taaatgaaag
      541 atctaaagac aatcaacacc gtagggatat ggagataaaa tcaatagagt caatagatta
      601 tttatcgata ataacactaa aaaagccacg ttcctaaacg tgacttacgc tgaacgatat
      661 gtgttgtttg gtagacgaac aaatcgttca ttataaatat atgatggtat cctaatatag
      721 taagcgtggc tagcatacct ttaattactt taccttttac agttaccata cacatttttt
      781 gagcagaaat caagtaaata aaaaaatatt atatttcaca acatgctaat agactcatat
      841 ctagaacgca ttaagtattt acctgtctgg ttcgctgaga cttctttttt aactttcttc
      901 aattttcttc tttattctaa aaagcgtact gcgactgaaa ccggttatag cttcaacttc
      961 cttatacgat ttacctgaag taagcagttc atatgcgtgc ctcttcttcg gtgtgattgt
     1021 tgctttcgga cgtccttctc tgaaattagg attattcttt cttgcaaaca tttttccttc
     1081 ttgggtacgt tccacaatca tatcacgttc catttctgct acactcaaca acgtccgtgt
     1141 caccattcgc cccatggacg tattttcaat cgttcctaga ttcaaaacct tgatcgttac
     1201 gttatcatcg agtaaaggtt caatgatctc taatgcttct ctcgtgttac gagcaaatcg
     1261 atcaagcttc gtaacaacta tttcatctcc aggcttcaga ccagccaaca gctgattgaa
     1321 ttttggccga tcccgttttg tacccgtaaa tttttctgaa aaaatattct tcttctctac
     1381 gccgttctca agcaactgtt gaatttgttc ttttgtactt tgagaagcgg ttgagacacg
     1441 tgcatatcca tatttcgtca tttggtccac ctacttttac aaatcatcta tcgtatactc
     1501 agaataccct tcatagaaat agctgacatc gattcctttc atatataact cgcgatcgtc
     1561 aattttatca gtcagagcat tggaaattag atagcgtatt tccaaatcgt ttatgggact
     1621 tcgctccatt gcagaaagat agtctgcttt atcgattaag ttccaatcta caactttttg
     1681 caattcttcc tttaaaatga gatccagcca aattctagta cttcggccat ttccttctcg
     1741 aaatgggtga gcgatattca tctccacata tttcttgata atctcttcaa aagaagtttg
     1801 tggcatctga tcaatatgct gtaacgaatg ttctaaatac ataactggtg caaatcgaaa
     1861 atttcctttt gcaatattga ccgatcggat ttttccggca aaatcgtaaa tatccgaaaa
     1921 tagaaattca tgaatttcag ataaaccttt aaatgtacca acttcgattt catttatttt
     1981 tccacaatcg tacaattctt tcgctttttg tttactgagt ttttcttcaa ccttagccaa
     2041 ctcaacttga ttggttaatc ccaatttatt ctctaaaatc atgcgaaatc tccttaactt
     2101 tatgacataa gtttatgaaa cactctcaac cccattatat cgcgattttg tttatgtgtc
     2161 aaaaactatt aagtttctga cccccgatag atttttcaag tgtctctatt ttgcacagtc
     2221 tagttgacct ttgatacacc tctatattta tagagaatct aaaacatcaa catggcatac
     2281 tcaaaattct aaatattagg gataaagaat tctcctatac aatcaaaaat ttcctttatt
     2341 tcgaactccg gtcacctaac tctattgatc tcttacaagc caaagaatat ctctatacta
     2401 caaagaaaca atagggtgcc tggttggtct ctttgtgttt tattttagaa tctagaacgt
     2461 aaatattctt gtcattctga ttttatctaa gaatatcaat taaatctttt tttgcagcct
     2521 ctgaagcggg cattacagaa aagaccagtc caataaccga agatacccca accgctagta
     2581 aaatagtaaa tagatcaagt gacacgttta cctttatcaa agaaccaaca ctataagcaa
     2641 gagccattcc taataaata
//
LOCUS       DAZBSA010000182         2653 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000182 DAZBSA010000000
VERSION     DAZBSA010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.188"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            677..763
                     /locus_tag="ACHGMT_002798"
     CDS             677..763
                     /locus_tag="ACHGMT_002798"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFN6836482.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1065..1289)
                     /locus_tag="ACHGMT_002799"
     CDS             complement(1065..1289)
                     /locus_tag="ACHGMT_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836483.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRS
                     LEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1655..1939
                     /locus_tag="ACHGMT_002800"
     CDS             1655..1939
                     /locus_tag="ACHGMT_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836484.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1942..2358
                     /locus_tag="ACHGMT_002801"
     CDS             1942..2358
                     /locus_tag="ACHGMT_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836485.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(2495..>2653)
                     /locus_tag="ACHGMT_002802"
     CDS             complement(2495..>2653)
                     /locus_tag="ACHGMT_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFN6836486.1"
                     /translation="NPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPKI
                     SLRNWLEE"
BASE COUNT          873 a          495 c          426 g          859 t
ORIGIN      
        1 cgttttttca tccattcctt tcttcccccc ttacaaataa ataataacct ttaacaacca
       61 actcgtaaat aggttggttg ttattataca aaaggatatt agtactcaga taaaaagttg
      121 ttagtactca gataaaaagt tgttagtact cagataaaaa gttgttagta ctcagataaa
      181 aagttgttag tacccttctg tatccgttgg gagagtaagg ctcaactgcc ccctaaaaag
      241 ggttctaaaa aatctttaaa aaataaataa aataaacgcg cgcgcgcgag caaaatttcc
      301 cgccaaaaaa tcacccactt tttaaaataa acaggttcgc gcatgatatt aaactgccaa
      361 tctagctccc tatggtcgct gtaagaagga gtgatcgcta cgctctcaca ttttacttgc
      421 agcagtcgcc tttaggctcc ttcgccaaaa ataattcatt agggactcct ttgtggcgtc
      481 ctgagagctc gtctaagccg ttctaagaca agattcgata aaaactatgt ccttcgttct
      541 aaagcgcctt acaggcggtc agggcaagcc ctaacaaccc caaggcaata aaaaacttag
      601 gcttattttt gcttttttat tgtataattg attgtcgcct atccgacagt cttgcgactg
      661 gaaggaggtg tgtttcgtgt tcactaactt tatttgctcc cttcttgttg ggcttttaag
      721 cgctttgttc agttactggt tgaataaccg aaacaagaag taaaacttaa ttaaggcgac
      781 tcagcccaca aaaaaagccc cagagaatcc gacctctctg gggctttcgt gtgtttcgtg
      841 ttcacttatt tgcaatcaaa ttataacaga aataaatatt tatttcaaaa atatcctaca
      901 aaagtctaaa atccgtaatt atttcttata tgcaacattt acaaataaag tcgctctgag
      961 agctcgtatt tttcgattta agacgtttta tttagttttt ggtgtatttc attggtccaa
     1021 gctaaaaaac tcttagaagc gattttagag ccatttaaac ccacttaatt atattctcca
     1081 tgaaattcgc tccatgcgtc aaaagctgcg tctgaaattc cgccgtcttc gttcactagt
     1141 tgcattaatt tttccaacga tcgttttctt tcgggtgtca tatttttata ttccaacaat
     1201 ttttcgtctg ttaaatcgaa cttgattgct tgaattgctt gctctaattt ttctaagttt
     1261 tgaaaatatt cgtaaggaaa tttttccatt ttttctttct ccagtctcac aatttttcta
     1321 gaaataaaat cacttctttg ccgtctatac tctcaaaaaa aatgtcgttt gaaaaactaa
     1381 ataaattttt gatcgtgcat ttttaaattt ggttatcgat tacacgtgca gcactccccc
     1441 ccgaatttta aagagcataa ggaaacgtgt acccactttc taaaaaaagt gggtagatag
     1501 gctgtgccta tcacgtttct cagaaaaaaa tttgatatca ctttttcgcc atcgtgggta
     1561 gcttatgtat agcattttgg caaaaaacag ctatacaaat atagtatttg tatggtatac
     1621 ataaattata cataattgca aaaggagatt ttttatggac attttagttc ggaatattga
     1681 tccaaagttt gttgcaacaa tagacaaacg ttgccaagaa ctcacgaata aaacaggaga
     1741 aaaatggagt cgaaataaat atctaaaagt tttaatcgaa agtgattttg accgtcaact
     1801 tacgacttac aaaaaagacc aattcgattt attgctcgat aaatttattg ctatccaaac
     1861 agaaaatact caaatattaa gcgagtatgt ctacacaaat aatcaaatca ttgaaatgct
     1921 tatcggagga atgatataac catgagaaaa acttctgtcc aaaaacatgt tactttgtac
     1981 cctagtcatg ttgacgtttt agattccatc atcgaaaaaa atccagatat caataactac
     2041 tcggaagcca ttcgcttttt atgtatgagt tatgaacccg ataccaacac gaccactacc
     2101 agcaatcagg caaaactaaa tgctatggga aaagaaattt ccatcatgac tgaaatcatc
     2161 gcagaatttg ctgatttata tctaaaagat acaggcgttc ttattggcac aaattctaaa
     2221 gtatatcaag aagtgaaaga aaaaatcgaa gctaaactaa atccatcaaa tcgaaatcga
     2281 ccaaagaatc atcaaaaaga aaccagtcaa acagtcactt ctagaaattt cgacccaaca
     2341 aattttctaa aacgataaaa aacagatagg agttattcct atctgttttt tatttagaag
     2401 ccccatacaa tatgctagaa tgtactagca atcttagtcg gttgtaaggg acttgtctac
     2461 ttgcttaatg taaggggggc gagttctttt ttatttattc ttctaaccag ttacgcagag
     2521 aaatctttgg caaatctgac gttaggcttg aaaaagtaaa ttctaatttg gcgattttat
     2581 tgcccttttt tgcttttatt tttgtgactt taagatcatt gaagtatttt tttagttctc
     2641 gtaatgcagg att
//
LOCUS       DAZBSA010000183         2616 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000183 DAZBSA010000000
VERSION     DAZBSA010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.189"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            11..1462
                     /locus_tag="ACHGMT_002803"
     CDS             11..1462
                     /locus_tag="ACHGMT_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFN6836487.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1811..2443
                     /locus_tag="ACHGMT_002804"
     CDS             1811..2443
                     /locus_tag="ACHGMT_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFN6836488.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
BASE COUNT          978 a          378 c          513 g          747 t
ORIGIN      
        1 ggtaaatcaa atgaaaaaat ctttaaaact tgcatcagca gttatattaa tgtcccaact
       61 ccttatagca acacctttcc atgtgttggc aagcgaaaat tcaacataca aaattgcaaa
      121 agaaagaacc gagacgactg aaatgagtaa ttccgatgta acagcagaga gtagtgaact
      181 tttgacaact tcatcaagtg aaagtagtac gacttcaaat gccgagtctt caacaacttc
      241 atcaagtgaa agtagtacga cttcaaatgc cgagtcttca acaacttcat caagtgaaag
      301 tagtacgact tcaaatgccg agtcttcaac aacttcgtca agtgaaagta gtacgatttc
      361 aagtaccgaa tcttcaacaa cttcaagtag tacaagtgaa ataacttctt cgaaagtgac
      421 aaaaccaaac catatagaat cttcaaaaaa ggaagcgcca aaaacggaag gacaaacaaa
      481 aactttttcg acaccacagc aaatagaatc aacagaatct ttccaatcac aaccaatact
      541 atctggtaat gaagaaattc attttgacaa aaatgaatct gtagaaaatt ttattatgaa
      601 aattggtgag tctgcgagaa aaattggtca gaaatatgat ttatatgctt ctgttatgat
      661 tgcacaagcg atcttagaat ctgccagtgg tcaaagccag ttagctcagg caccgaatta
      721 caatttattt gggataaaag gaacttataa tggaaacttt gtcattatgg taacaaacga
      781 agatttgggt aatggaacgc tttatacgac ccaatcaaaa tttagagtgt atgaaaacta
      841 tgaggaaagc tttgaagatt atgccaaatt attaactaag ggaattagtg gaaataaaga
      901 tttttatgca ggagcattaa aagctaatag taaaacatat cgagaagcaa ctaaattcct
      961 tacaggacgg tatgcaacag atacacaata ctatttgaag ctaaatgaat taattaaaac
     1021 ctacgattta accaactatg ataaagaagt taaaggtcca caaatgagta agactggata
     1081 ccaagtgcct attcataatt attcaataac cagtttgttt ggaaatcgtg gaaatgaatt
     1141 tcaccgagga cttgatatgg cagctacaca gggtgagccg attcatgcag ttaaagctgg
     1201 taaggttgtt aaagcagagt ttcactactc ttggggaaat tacgtggtga tacaacacga
     1261 agatggatca accgcgttat atgctcacca gcaacaatac ctagtaaaaa ctggagataa
     1321 tgttttgcaa ggacaaataa ttggctatgt aggaagtact gggaatagta ctggcccaca
     1381 ccttcatttg gaaatttgca aagatagtag tctttctcag agcatgttag ttgatccgaa
     1441 aaccttatta ttcggagact aaaaaaggta atcaatctga caaagtaaca agaaaatggt
     1501 tggattctta gacctttgat caggatattg agactggaat tgatatctcg attacgatgg
     1561 gtttgacaaa taaaacaagt ccattatcga atatcgagtg tattattgcc gtctttatgc
     1621 tcatattttg agcaggtttc actagatgaa aacaatttat ctatcttgag gatttcgtgt
     1681 acgtatcgtt cagtcttgta ttgtaaatta gtcataaaat cagactaaga aaaattggaa
     1741 atattttttt ctgatttatt aaaagttaat tgaaattata tggtacttct ttcttgggag
     1801 ggtaggtgca gtgaaggaaa gatatggtca ccttactgtt gttgaggaga ttactcataa
     1861 tggatataaa aaatatcgtt gcctatgtga ctgtgggaat gaaacgattg tatatcgatc
     1921 aaacttacta tcaggtagaa ccaaaagttg tggatgtgga aattataaaa ataggcataa
     1981 atataaaaat ttgacaaata aaacttttgg taagttagtt gcaatcaagc caactaaact
     2041 gcgaagtgaa ggaactgttg tttgggaatg tcaatgtctg tgtgggagaa aaaagttagt
     2101 atcggcaaag cgacttctaa aaggtgatgt aaaaagctgt ggatgtttga aatcaaaaaa
     2161 gcatcacatt gaaaataaaa aattcggatt acttactgca aagtatcctt tggacaataa
     2221 ctgttcatgg aaaacaaaat gggtttgtca ttgtgagtgt ggcagtatgt gtgagataag
     2281 ttatagtaac ttattttttg gacatactca aagttgtgga tgtttaaaaa ggcaagaaac
     2341 aaggacatgt gttgaaggga cagtaataga gtgtctaacg tcaaaattac cgaaaaataa
     2401 tacaagtggt gttaagggag tttatcgttc taaagagaaa tagattgcgt atataacttt
     2461 aaaaaaaaag aggtactatt taggatcgta tgattctatt aagagtgcag ctcaagctag
     2521 aaaagatgct gaaaataaat tgtttcaacc tttgattgac aaatatcgag gtaatcatta
     2581 attattgtgg ataataatta acagtgatat ataaac
//
LOCUS       DAZBSA010000184         2613 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000184 DAZBSA010000000
VERSION     DAZBSA010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.190"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            316..570
                     /locus_tag="ACHGMT_002805"
     CDS             316..570
                     /locus_tag="ACHGMT_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFN6836489.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="ACHGMT_002806"
     CDS             571..888
                     /locus_tag="ACHGMT_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFN6836490.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="ACHGMT_002807"
     CDS             1447..2382
                     /locus_tag="ACHGMT_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836491.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
     gene            2476..>2613
                     /locus_tag="ACHGMT_002808"
     CDS             2476..>2613
                     /locus_tag="ACHGMT_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFN6836492.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LK"
BASE COUNT          958 a          375 c          482 g          798 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagagaaaaa
     2401 tgtctaaaca tgttggtaaa tcaacaaaaa aacgcatata gtagtctcaa aactcccaag
     2461 aaatgaggac tacatatgcg ttcatcaaaa tatactgaac attatacaga tacttttata
     2521 acattcaagg aaattatgag aactgtttct aacatatacc acaattgtgt accagataag
     2581 attaaaaacc gaagaaatac tgatcaactg aag
//
LOCUS       DAZBSA010000185         2492 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000185 DAZBSA010000000
VERSION     DAZBSA010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.191"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..435
                     /locus_tag="ACHGMT_002809"
     CDS             <1..435
                     /locus_tag="ACHGMT_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFN6836493.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="ACHGMT_002810"
     tRNA            complement(759..842)
                     /locus_tag="ACHGMT_002810"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="ACHGMT_002811"
     CDS             1370..1870
                     /locus_tag="ACHGMT_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN6836494.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="ACHGMT_002812"
     CDS             complement(2060..2404)
                     /locus_tag="ACHGMT_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFN6836495.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
BASE COUNT          776 a          478 c          413 g          825 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt tt
//
LOCUS       DAZBSA010000186         2406 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000186 DAZBSA010000000
VERSION     DAZBSA010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.192"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..1147
                     /locus_tag="ACHGMT_002813"
     CDS             2..1147
                     /locus_tag="ACHGMT_002813"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFN6836496.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1318..1776
                     /locus_tag="ACHGMT_002814"
     CDS             1318..1776
                     /locus_tag="ACHGMT_002814"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFN6836497.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1935..2405
                     /locus_tag="ACHGMT_002815"
     CDS             1935..2405
                     /locus_tag="ACHGMT_002815"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFN6836498.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          800 a          454 c          455 g          697 t
ORIGIN      
        1 catgcacatt caaacaaaat tgatccacgg aggcatcagt gaagatccca caacaggagc
       61 tgtcagtgta cctatttatc aaacatccac ttatcggcaa gatggcgtcg gccagcctaa
      121 acaatatgaa tattcaagat ctggtaatcc tactcgtttt gcattggaag aactgattgc
      181 tgatttggaa ggcggtgttc gaggattcgc tttttcttct ggtctttctg ggattcatgc
      241 cgttttttct ctttttcaag ctggcgatca tatcttatta ggagatgatg tctatggagg
      301 aacattccgt ctatttgaca aagttcttac taaaaatggt ctcgagtaca cgatcataga
      361 tacgagtaat cttgataaaa tagaacaatc catcaaaccc aatacaaaag cactttatct
      421 tgaaacacct agcaatccgc ttctaaagat tactgattta gaaaaaagtg ccactctggc
      481 tcatcaacat ggattgatag ttattgctga caatacattt gctactcctt attttcaaag
      541 acctttagat ctcggttctg atatcgttgt ccatagcggt accaaatatc taggcggaca
      601 cagcgatgta gtagctggtt tagtgacttc taaccataaa gatctagccg atcagatcgg
      661 attttaccaa aatgctatcg gagccgtact tggtccccaa gatagctggt tacttcagcg
      721 cggaatcaag acactaagtg ttcgtatgga ggaacatcaa aaaaatgctt ttgtggtagc
      781 tgattttcta ttttctcatc cagcagtaga aaaggtctac tatccaggat tacccgatca
      841 tgaactccat ggagttgcta agcagcagat gagcggcttt agcggaatga tttcttttac
      901 tttaaaaaat gaagaatctg ctattccatt tgttgaatca ctccaactat ttactttagc
      961 agaaagttta ggtggcgtag aaagtttagt ggaaattcct tctgtcatga ctcatgcgtc
     1021 tattccaaaa gaaaaacgag aagaggctgg cataaaggac ggcttgatcc gtctttctgt
     1081 aggaattgag tatggtcagg atctcataaa cgatttagct caggcatttg acagaatcaa
     1141 gaactagaag tcccaataaa cgacttagca gaaaaatcta aaaaatattt cagattgcta
     1201 caccttggaa acgataagtg ttagaatcaa gtagaaagac gtttttgtaa atttcactaa
     1261 ataattcttt agtgaaaagc atcaaaacga atgaacaaac agataggagt cttacaaatg
     1321 agaatgaatg tcgaaagttt taatcttgac catacaaaag tcaaagcacc ttatgtacgt
     1381 gtagctgatc gaaaaacggg tgcgcatggc gatgtcatca ttaaatacga cgtccgcttt
     1441 aaacaaccaa ataaagaaca catggacatg ccaagtctac attcacttga gcacttgact
     1501 gccgaactaa tccgcaatca cgcagattac atcgtagact ggtctccaat gggatgccaa
     1561 acaggctttt acttgactgt attgaatcat gaaagctacg aagacatttt agatgttctt
     1621 gaagcaacaa tgaaagatgt tcttgaagct actgaagtac ctgcaagcaa tgaaacacaa
     1681 tgtggctggg ctgccagcca tacactagaa ggtgcacaag ctttggctcg tgaattttta
     1741 gcaaaacgcg atgaatggtc tgaagtagaa gcatagtttc taaaagaacg taaagatgag
     1801 aagcaatata gctatcttat cttcgtattt tattaaatat ttattgtctg aaaagagttc
     1861 taaacgggaa aacaaatagc tagttttcag tttagcgctc tttttattat ataaaataga
     1921 aaggagaaaa ctttatgtct atttataatt ttgaaactac tttagaagac ggcactgtct
     1981 attcattgaa aaaatatgca ggcaaaccga taatcattgt taacactgca acaaaatgcg
     2041 gtctagcacc tcaatttaaa caattagaag aactttacca agcttatcag acacaaggtc
     2101 tggttatctt aggctttcct tccaatcaat ttaaacaaga gctagattca gcgactgaag
     2161 cagcagaagc ttgtaaaacc acttatggcg ttaccttccc tatgcatcaa ttgattgacg
     2221 taaatggaga aaatacagat cctcttttta cttatcttaa aaatgaagct cctggtactg
     2281 tcactaaagc aataaaatgg aattttacta aatttttgat tgatagaaat ggcaaagtaa
     2341 tcgaacgatt tgcaccacaa acttctccat tgaaaatgac cgctgcaatt gaatccgttc
     2401 tttaac
//
LOCUS       DAZBSA010000187         2405 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000187 DAZBSA010000000
VERSION     DAZBSA010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.193"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(4..249)
                     /locus_tag="ACHGMT_002816"
     CDS             complement(4..249)
                     /locus_tag="ACHGMT_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836499.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(266..1117)
                     /locus_tag="ACHGMT_002817"
     CDS             complement(266..1117)
                     /locus_tag="ACHGMT_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFN6836500.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            1283..2287
                     /locus_tag="ACHGMT_002818"
     CDS             1283..2287
                     /locus_tag="ACHGMT_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFN6836501.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT          774 a          405 c          485 g          741 t
ORIGIN      
        1 tttctatttt ttcggtatca aggcaaaaac aggtagttca ggtgaataaa tgacttcatg
       61 ttcgtgccag ggtttgacca ttagcgaatg atcgaaccgt tttttcagtt cttcgatgat
      121 ttgatcatgt gtccagtttc ttgcaggaaa atgttggatt ttctctgcat cgatcaaaaa
      181 gacaaaatcg gaatgaaaag tctgggtaac ttcttccgag agagaattca agcgttcgat
      241 tggatccata aaatcacctt tcgacttaaa attgggtagt atctgggtct tttgctttgc
      301 cgactacacc atctaactga tcgatttgtt tcatttcttc ttctgttaat tcaaaatcaa
      361 ataattcagt attttctttg attctgcttg gtgtcactga cttaggaaga ggaaggaatt
      421 cgtgttgcag cgaccaacgc aaagcaactt gtgcaatcgt cttgtcatgt gcttcagcga
      481 tttgtttcat ttcaggcaca tcgaagattt taccagtgcc taatggacta tatgcttcga
      541 ggatcatatc atgttttttt gcatactcaa ctacagttgt ctgtaattcg cctggtgcaa
      601 ggaaaacttg attgaccatc ggcataattt ttgctgtttt tgctagtgta tctagatggt
      661 ggggtaagaa attgctcaca ccgattgctt tgattttacc tgcttcatac agttcttcaa
      721 atgctcgcca tgtatctgca tttgcttgtt cccaattatc acgaaaagca acagggtttg
      781 gccagtggat caaaaacaga tctaaataat ctgtttggag cttcttcaat gattcttcaa
      841 aagaagacat cacaagttca tagctgtggt ttgcattcca taattttgtt gtcaagaata
      901 tctcttctct aggaattcca ctttccttaa tcgcttggcc gacgctttct tcattcttgt
      961 atccttgggc agtatcgata tggcgataac ctgcagccag cgcttcttta acagaggaaa
     1021 cagcgacatc cccatcaggt gtttgccatg tgccgaatcc tactaccggg atttcgtaac
     1081 catttgatag acgataagtg cttgttaatg aattcatact ttcagctcct tttttattcg
     1141 ttcaactata agtgtagcgc atttttcgaa aaaaagaatc cattttgttt ctactcttga
     1201 aatttttgtt taaaagaaaa tgattttgat tctggtattt ttgatttttt ttccgtaaac
     1261 taagacagaa gggaatgaga aaatggatat ctatttaaaa aaagcaattc tgcatattat
     1321 tgacagagaa actggttctc cagtcttttc acaaaaagag ctggatctga caaaagaata
     1381 tattcgtgat tttcttcaaa agaagatcca aaaaatctct tctgctcaaa caaaaactgg
     1441 acaactagca gaagaaagca tgatcgttga atcacttaaa atcgctcaag aaaattttgt
     1501 ggaagcaagt gaagcaatcg ttcagcggtg gttcgatatc taccaagaaa gtgaagaagc
     1561 acctagtgca gatgtatttg ttgtcttgta tgaattagat acagtgatgt acttatcttt
     1621 attgaaagtc aattatcgtg aggcttacac tcactttgtt gaagcagacg aagcagggat
     1681 cgacaaccgc ttgattttga atcgagcaat tctaggggcg aaaacacaaa aagcagatga
     1741 agcattcgct gtgaatttga ctgattggac ctacgaatta attgaaaaaa aatatgagtt
     1801 ttctggcaaa aaagagcttt atttttctgg aagagtgatc gaaagtgttc ctgcaccttc
     1861 tttagaagaa aatgtaaaag tgatccaaaa agtagccaag catcttggaa aaaaatttga
     1921 gacagaagaa tttgatatca tggctgattt gaaagaagct gtctatgata cgatagaaga
     1981 aaaaggtcgg ttggatcatg agatgattgc ggaaaaagtc ttcaaggaaa acatcacggc
     2041 aaaaatggct tttaaagaag aagtacaaga gcaaggattt gtcccagaag cacctcctgt
     2101 aaaagaagta caagaaattt ctgagaaaaa atttggaaaa caaaaattca agctatcaaa
     2161 cgggatcgaa ctgatcgtac cagtagatgt atatcgcaac ccagatttga ttgaatttgt
     2221 gaataatccg gatggtacga tatccgtgat gatcaaaaat gtggatgaag tattgaatcg
     2281 tttataaaaa tgactagaat attaggtgtt caagtaccag tgatcagtta aatgaattgt
     2341 gtcaagagaa ggatcggaaa agacgaatat aacaaaaaaa gagcgtgaga caaaagtaaa
     2401 gaaca
//
LOCUS       DAZBSA010000188         2400 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000188 DAZBSA010000000
VERSION     DAZBSA010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.194"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..2069
                     /locus_tag="ACHGMT_002819"
     CDS             207..2069
                     /locus_tag="ACHGMT_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN6836502.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="ACHGMT_002820"
     CDS             2069..>2400
                     /locus_tag="ACHGMT_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFN6836503.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DAZBSA010000189         2303 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000189 DAZBSA010000000
VERSION     DAZBSA010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.196"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..1750
                     /locus_tag="ACHGMT_002821"
     CDS             137..1750
                     /locus_tag="ACHGMT_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN6836504.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          721 a          408 c          447 g          727 t
ORIGIN      
        1 aaatttttag gttttatcca caaattattt ctctatattt tctgtctatt caaaataaac
       61 aataattttc cggcacaatt atttgctgtt ttttaccata acatagtacg atcaacgtag
      121 aaaaggagat gtttttatga aagttgttgt cattggcggt gtagctggcg gtccttcatt
      181 tgccactcgt ttccgtcgat tgaatgaagc gcacgaaatc attatctatg aacgcggaga
      241 aaatatttct tatgcaagtt gtgctttgcc ttattattta ggaggtgtga tcactgaccg
      301 tgactcgctg atcgaacgta caccagaaat attgaaaaca aaaaacaaca tcgatgtctt
      361 tactaaacac gaagtaacag caatcgatcc ttctactaag cgattaacag ttaaagacct
      421 atccacaaat gaagaaataa aaacagatta cgataagttg atcatctctt ctggtgctag
      481 accagattat ccggatattc ccggagtttt tgaagcagaa aacggctttg tcctccgtag
      541 tgtgacggat gcggatcgaa tcaaatcgtt ccttgaagaa aaaaatccac aacatgtcgt
      601 cattcttggt gcaggtgtta tgggtctgga attagctgag aatttcaagc atcgcggctt
      661 agacgtgact ttaatcgatc aattgccaca agtcgctttc ccttatgatc cagaaattgc
      721 taatttagtt tatgacaaat tgctgaaaga aggattagcc gttcatttag aaacaagagt
      781 tactgagatc cgtgataaag gtcgagaaat agtattatca gatggctccg tcctttctgc
      841 tgatatgcta atttttgctg ttggtgtttc tccgaagaat gaagtggtga aagcaaccgg
      901 catacaatta tcagatacag gtcagatcat tgttgatgac cagttacaaa ccaatctgcc
      961 ggacatctat gcgattggcg atattatcga aacgactagt gtagtgactg gtcagccgat
     1021 ccaaagtatg ctttccagtg cggccaatcg tcaaggacat atgctggcag atattttaaa
     1081 tggtacgcct atgcgctatc gcggatatat tggtgcaggt gtcgcaaaaa tctttgatca
     1141 tacagcaagt tatgcgggaa tgacagaaca tgaattaaaa gcatcaggca taacaaatta
     1201 taaaactgtt tttatcactc cctttgacca tgcctatttc tatccagaag ctacaagatt
     1261 aaatctaaag ctgatttttg atgcagatag cggtcgtatt ttaggtggac aagcatttgg
     1321 agaaaaaggt gtcgataaac ggatgggaga actttctgta gcaatcaccg gaaacttgac
     1381 agtctttgat ttgcctgatt tggagttgcc ttactctcca ccgtattcta ctacccgtga
     1441 cccgttgaat atagctggtt atgtcgcaat caatcaaatg acgaatatcg tagaaacgat
     1501 caaagcaagt gatatacccg aaaacgattt gaaagaagcg ttctttttag atatacgtga
     1561 acctaataaa gcaccttctg gcagtatctc ggcaacaaaa aatattccaa tgaatgagtt
     1621 acgagatcga atcaatgaaa tcccaaaaga taaaaaagtt tacattactt tcagaagagg
     1681 attgaatact tatacttctg cccgaatctt ggcaggtttg ggtatcaaag cgattttgat
     1741 tgaagaataa aaaaacgacc gcggtgggta gccgcggtct aggagttatt tttacttgag
     1801 gagtaaaaat gaaaaagtat atagggttgt tggttggtat gtatatagaa tagaagataa
     1861 atgtgaagaa attgtgcatt atttcttcac atttattagg ttatagttta agaaagttta
     1921 aacattgcgc aatttaatta ttttagctgt ttcttttcat aaaaaaacat gttgtgactc
     1981 tgagacaaat catagtccat agtgatgtct ctagagtaac tacgcctaat attacgaggc
     2041 atcatattaa tctatcagca ctagtaccta aactagcatc cctttcgaat attttttctt
     2101 atataataaa gtttgctttc actcctctaa tctcaccaaa tcgactggct tgcgttgtgt
     2161 gatactagga actgattggt ttagaacgac ttttctattg taatcatatt tttcgccatc
     2221 ctcgttgtct tctaatcctt caatgaatga tccctaatgt gtgtattaaa taatttaatt
     2281 tttttgttcc tatttattta aca
//
LOCUS       DAZBSA010000190         2299 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000190 DAZBSA010000000
VERSION     DAZBSA010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.197"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2149
                     /locus_tag="ACHGMT_002822"
     CDS             <1..2149
                     /locus_tag="ACHGMT_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFN6836505.1"
                     /translation="KDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFFII
                     FTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKANQ
                     AITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKRLS
                     AKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGVTD
                     QELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLAKG
                     YSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSMNA
                     EFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEITE
                     NALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRSGQ
                     VALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFGLG
                     TTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQYA
                     STVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGFHQ
                     VVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEIAI
                     SVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          787 a          413 c          510 g          589 t
ORIGIN      
        1 gaaagatttt atgaaaaaaa tattaaatac ttcttggtta gaaaaaataa gaaaaggtgc
       61 acaagaacct aagaacaaaa cgtttcgccg gtcccatgtt ccttttcgct taaattttct
      121 ttttttcatt atatttacgt tatttgttgc gttgattgct agattgggtt atttgcagat
      181 cgtgaacggc gaagagatgg aggcgcgagt gaagtctact tctacgatta cggtccaaga
      241 aagtactcct cgcggaatga tctacgactc cacaggaaaa gctttagtta cgaataaagc
      301 gaatcaggcc atcacatata cgagaggaac acaagttacg gctaaagatt tgcttgaaat
      361 cgcaacgaag ctgaactcgc tgatcgatgt tccggtagat gaaaatctga cagatagaga
      421 taaaaaagat ttttggctgg ctgatcccaa acatttgaaa gaagccacaa aaagactttc
      481 ggcaaaagaa aagcaattag ggaacagcga acaatatagt gcgatggtca ataaagtcac
      541 agctgatgag attaatttaa acgaggagca attaaaaatt gctaccatct ttaaacggat
      601 gaatagtgca tatgaattaa acactgtcta tatcaaaaac tctggtgtta ctgatcaaga
      661 gttagctgtt gtggcagaac gcgcttctga acttccagga gtttcgactg gcacagactg
      721 gacacgagaa tataatgcag caagttcact gaaaagtatt cttggcagtg tgacgactga
      781 aaagcaagga cttccagcag atgaagcaga gaaataccta gcaaaaggtt attctcggaa
      841 tgaccgtgta ggacaaagtt atttggaaaa acaatatgaa gatgtcctgc aaggaaccaa
      901 aacccaatat gaagtttccc ttgataatga aggaaatgtt tcgaatcaaa aagaaatctt
      961 ctctggggaa aaaggttcta atttaatgtt atctatgaat gcagaattcc aatcaaaagt
     1021 agaagaaatt ttgaaaagga attatcaaac attgatcaac aacggcaaag cacagtattc
     1081 gcctggtgct tatgcagtgg ctatgaatcc tcagacagga gaagtgctag caatgactgg
     1141 tttcagtcat gaacaaggtt ccaaagaaat caccgagaat gctttaggca cgattactag
     1201 cgcctttgat ccagggtctg tagtcaaggc tggtactttg acagcaggct gggcaagtaa
     1261 cgcgatcagc ggaaatcaag ttttgattga tgagccgatt cgtctgcaag gtgcttcaga
     1321 aaaaagctct gtctttaatc gaagcggaca agttgctctt gatgcagtca aagcatcgga
     1381 attgtcttct aatacgtata tgatcaaagt ggcgttaaag atgttaggac tagactatac
     1441 acctggaatg ggactaccta gtttagatga agaagctaaa gcttatcagc aattgagaga
     1501 ctctttcaaa gagtttggtc ttggtacaac gactgggatc gatttgccga atgaatctcc
     1561 tgggatctca cgttctgttg attatatgaa gaagttcaat gcggataatg gcaaagaatg
     1621 gtatgctcca ggtaacttta ccgatttggc atttggacag tttgacacat atacaccaat
     1681 ccaattagct caatatgctt caacagtcgc taatggcggg aaacgagtac agccgcacct
     1741 agtcaaagcg atctatggca atgacgaaaa tggaaacttg ggtgaagtga agaaagaaat
     1801 tggcacaaca gtagaaaata cggtcaatat ttctgcagaa aatatggcga tcctccgtga
     1861 aggattccac caagttgtcc atggtaccga tccttacaca acagccaaac cattagccag
     1921 tgcgaaaatg gacctttccg caaaaacagg gactgcagaa acggttgcgg aaggtcatcc
     1981 agatattgcg acagtcaata gtaatatcgt ggcatacgga ccaacagaca atccggaaat
     2041 tgccatcagt gttgtcttgc cgaacttatt agacgaacaa gaccacataa acttgacgat
     2101 tgctaaagaa ataatggacg cgtattatga tatgttcatg gcaaaataaa aaagaggtcg
     2161 tgaaaaagcc gcttagtttc gagtatgaag aaaaattttg tgaaaatagc ttctcacatt
     2221 tttgcttaaa tttgacttca taccgagaaa ctggcttttg aacaccgttt actcggcaaa
     2281 aaaggttgtg acaaaaatt
//
LOCUS       DAZBSA010000191         2289 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000191 DAZBSA010000000
VERSION     DAZBSA010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.198"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            249..929
                     /locus_tag="ACHGMT_002823"
                     /pseudo
     CDS             249..929
                     /locus_tag="ACHGMT_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
     gene            1069..1608
                     /locus_tag="ACHGMT_002824"
     CDS             1069..1608
                     /locus_tag="ACHGMT_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836506.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
BASE COUNT          844 a          325 c          409 g          711 t
ORIGIN      
        1 caggatacaa atttatttaa gcatatataa atataattga aataggacat aaatatatga
       61 gtatgagaaa ggtgattcca tgtttaataa atgggcaaac cacccaatga actcgagagg
      121 tagagataat ttgaaggaga cggacaagtt ctgggaagaa gtatatagta aaaacggttc
      181 tgttgcaaag ttttccaaaa aatctatttt agtgtaaaat tgagaaaaaa gacagagagg
      241 acagagtaat gaatcatttt aaaggcaaac aattcaaaaa agacgtcatt attgtcgctg
      301 ttggttacta cctgcgttac aatctaagct atcgtgaagt tcaggaattg ttatatgatc
      361 gtggaataaa tgtttgtcat actacgattt atcgttgggt ataagagtac agcaaagtcc
      421 tctattatct ttggaagaag aaaaatagac aatccttcta ttcatggaaa atggacgaaa
      481 cctatatcaa aattaaggga cgttggcatt atctttatcg tgcaattgat gcggacggct
      541 taaccttaga tatctggtta cgaaagaaac gggatacgca agcagcctat gctttcttaa
      601 aacgactcca taaacagttt ggtgagccga aagcaattgt gaccgataaa gcaccttctc
      661 ttggctccgc ctttagaaag ttacagagtg tgggtttata tactaagaca gagcaccgaa
      721 ctgtgaagta tcttaacaat ttaatagaac aagaccatcg acctattaaa cgacgaaata
      781 aattttatca aagtctccgt acagcctctt ccacgattaa gggcatggag accattcgag
      841 gaatatataa aaagaaccga agaaatggaa cgctcttcgg cttttcggtg tctactgaaa
      901 tcaaggtatt aatgggaata acagcctaag atatttggag ttcagagagg gcgcgtttga
      961 ttttcaaact tcgcaacaga accgagaacc ttgggttttc aagtgctttt tggttcatta
     1021 atcttaacca tagtgttttt aaagtggaaa acaagcaaca aaaaaagtat gtggagcctt
     1081 gtaggaatag ttcttattat ttttactgtg actgcagcaa cagaagcaaa ttattcgcat
     1141 aaaattcagt cgtataaagg tcgtttagtt atgaccaaaa aagatgttac acgttataca
     1201 tcctcaagaa ctggaggcct atatgactat gcaccattag atgcattgaa atataaagat
     1261 catttaaaga aacatgaagt aaaagttggt gatcggtggg agaatgcatc tcataaagca
     1321 tataattcca aaatagtcta tacagtagaa gatgccgatg agcaaaatat cacattacct
     1381 gtattcaatt attggggaac agttgtgaaa gtaaatggga aaaagagcac tatagaaaat
     1441 gacaatggtt tagtgtctat taaaggtacg gggaaaagaa ctatagttga ggtttcatct
     1501 acgtatccaa ctacgatgat tctatcttta ttaattagtt caataacttt tatattctta
     1561 tttacacgtt atttaataag atgctgcggt gaaatgaaaa tagattagat agatattata
     1621 aatatgttga ttctattttc atagcaatgg agaaaataca ttagcaatat ataaaagaag
     1681 gtatcaagag tcacagtgaa tttattccga aaagtgaaaa gagcaaatat aaaatttaat
     1741 gaaaatatag aactaaatag gaaaacgtag acgtaatttt ctacgttttc cttttcatat
     1801 atattaaata ttacggaaaa ttatataaac taatttagtg ttttgtttgt aattcctaat
     1861 tttgctccac ttttagataa tttttaggtc tatttaactc atcagacatt ctatgctcat
     1921 ttttctccaa atttgtttca actctttcgt acttaatttt gcgtatttga aaattcttct
     1981 tttagagtac ttttattacg atactagtat gatatatatc tatagcaaac agttaaaatc
     2041 ttcctaaaat aagcgaatag cattcctaaa acctataaaa tttttagatg aagcgacaag
     2101 tggacaatta ataaactact tgaacatgaa attatggatc acctttcaaa tttagaaaat
     2161 ggaattctta tacttattgc gcaacattta ttgattgcaa aattatgtga caaaaaaaac
     2221 tagtagaaga aagaataatg gcttgataat aaagtactac ctattaaagg tagtacttta
     2281 tttaattta
//
LOCUS       DAZBSA010000192         2222 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000192 DAZBSA010000000
VERSION     DAZBSA010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.199"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..536)
                     /locus_tag="ACHGMT_002825"
     CDS             complement(<1..536)
                     /locus_tag="ACHGMT_002825"
                     /inference="COORDINATES: protein motif:HMM:NF026247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="HFN6836507.1"
                     /translation="MDTEASLEINQEKSVSQKKWSTSNKILSGVLLLIMWIIMAFNTQ
                     NADYYAYSVLYNGIAQFGMDFSSDSIEPGFRAVMYVCTFISSQYQIFLLIISTVLLSA
                     LLYVIRLFTTNVFLVLFLFFIFGFTSQAIQVRMFSALIFILLAFYHLTKLRGSKAIFK
                     YIFFILVAGLFHNLAYFYL"
     gene            complement(508..1404)
                     /locus_tag="ACHGMT_002826"
     CDS             complement(508..1404)
                     /locus_tag="ACHGMT_002826"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN6836508.1"
                     /translation="MKNKIGIIIVTYNPDNNIKLLVDTLIQEKQNKLIIVDNGSDNIN
                     SFQFEMMENLILKKMNENIGLATAQNIGIEIAKKEKCDYILLLDQDTIIQKNLSLKLL
                     EEFTNIEAKDKDIGIIVPNYFENSNDESVSFTCKNERGRYVKKHFASTSGSMKIAFAN
                     SSGMFFKSELIDDVGNMKDEFFIDQIDVEFCKRVANNGYNIYVTNQVYFYHHVGDGEN
                     LKIGSKKIKIDNHNSTRKYFIARNSTLVKRMYKSDKEFSRLMNKRLLKFFIAIAFETD
                     KLSRFRAAIRGVRDGYRGVIGD"
     gene            complement(1394..>2222)
                     /locus_tag="ACHGMT_002827"
     CDS             complement(1394..>2222)
                     /locus_tag="ACHGMT_002827"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836509.1"
                     /translation="IAKFSRCNRVIFDVYDLWPESMPGIGNKYKFFTDLWKKLRDQYL
                     SNSSLVITECDYFKKFIKISGESKNMTIYPCSFNVDDQNDYGEKIVNKSLESTLNLAY
                     LGTINNIVDLEAINELLKTCKRKFGNKINLYFIGKGESKTKLADICEKNHIEFVDCGV
                     IYDNAEKKVILDKCHFGLNLFKSNTIIGLSLKSIEYFRFGLPIISNLSGDTKEIVKRY
                     NVGINYNSTVDENEMLKFYQCNKNINENVRKIYATIFSEQVLKMKWNEVYKVMNDEK"
BASE COUNT          750 a          391 c          261 g          820 t
ORIGIN      
        1 agataaaaat atgcaagatt atggaacagt ccagccacta agataaaaaa aatatactta
       61 aatatagctt tgctgcctcg caatttagtt agatgataaa aagctagtaa tatgaatatc
      121 aatgcgctaa acatacgtac ttgtattgct tgtgaagtaa aaccaaaaat aaaaaacaag
      181 aacaaaacta aaaaaacatt agttgtgaat aatctgatga catacaataa agctgagagt
      241 aagacagtag aaataatcaa taaaaaaatc tgatactgac tggaaataaa ggtacatacg
      301 tacataacag ctctgaatcc cggttcaata ctatctgaac taaagtccat accaaattgt
      361 gctatcccat tatacaagac agaatatgca taataatcag cgttttgtgt gttaaatgcc
      421 atgatgatcc acattatcaa aagtagcacc ccagacaaaa tcttgttcga tgtactccat
      481 tttttttgtg aaacactctt ttcttgatta atctccaatg acgcctctgt atccatctcg
      541 aacccccctt atagccgctc taaatctact gagcttatca gtctcaaaag ctattgcaat
      601 aaaaaatttc aataatcttt tattcataag gcgggaaaat tccttatcac ttttatacat
      661 tcttttcacc aatgttgaat ttcttgcaat aaaatacttt cgtgttgaat tatgattgtc
      721 tattttaatt ttttttgatc ctatttttaa attttctcca tctccaacgt gatgataaaa
      781 atagacttga tttgttacat aaatgttata gccattattt gcaacccttt tacaaaattc
      841 aacatcaatc tgatcaataa aaaattcatc cttcatattt ccaacatcgt caattaattc
      901 acttttgaaa aacattccag aggaatttgc aaatgctatt ttcatggaac cactagttga
      961 agcaaagtgt ttttttacgt atctccctct ctcatttttg caggtaaaag agacagattc
     1021 atcattgcta ttctcaaagt aattgggaac aataatccca atgtccttat ctttcgcttc
     1081 aatattggta aactcttcta ataattttaa agaaagattc ttttgtatga tggtatcttg
     1141 atctagtaaa aggatataat cacatttctc ttttttggca atttcaatac ctatgttttg
     1201 agctgtcgca agcccaatgt tctcattcat tttttttaaa attaagtttt ccatcatttc
     1261 aaattgaaaa gagttaatat tatctgagcc gttatccacg ataataagtt tgttctgttt
     1321 ctcttgtatt aacgtatcca ctaataactt tatgttatta tctggattgt aggtaacaat
     1381 aattatccca attttatttt tcatcgttca tcaccttgta cacttcattc catttcattt
     1441 tcaatacttg ctcagagaat atagtggcgt aaatttttct aacattttca tttatgtttt
     1501 tattacactg ataaaatttc aacatctcat tttcatcaac ggtactatta taattgattc
     1561 cgacattata tcttttaact atttctttcg tatctcctga taaatttgaa attataggta
     1621 atccaaacct aaaatactca atcgatttca gacttaaacc aattatggta tttgacttaa
     1681 ataaatttag tccaaaatga catttatcga gtataacttt tttttctgca ttgtcatata
     1741 taacaccgca gtcaacaaat tcaatatgat ttttttcaca aatatcagct aatttagttt
     1801 tgctttcacc ttttccaata aagtaaagat ttattttgtt tccaaatttt cttttgcatg
     1861 ttttcaataa ttcattaatc gcttctaaat ctacgatatt attaatcgta cctaaataag
     1921 caaggttcaa cgtagactct aagcttttat tgactatttt ctctccataa tcattttggt
     1981 catctacatt gaaagaacat ggataaatgg tcatattttt tgattctccg gaaattttta
     2041 tgaatttttt gaaataatcg cattctgtta taactagact agaattactg agatattgat
     2101 ctcgtagctt tttccataaa tcagtaaaaa atttatattt gttccctatt cccggcattg
     2161 actctggcca taaatcatac acatcaaaaa taactcgatt gcatctacta aacttagcaa
     2221 ta
//
LOCUS       DAZBSA010000193         2189 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000193 DAZBSA010000000
VERSION     DAZBSA010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.200"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..912)
                     /locus_tag="ACHGMT_002828"
     CDS             complement(<1..912)
                     /locus_tag="ACHGMT_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFN6836510.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1340..1903
                     /gene="efp"
                     /locus_tag="ACHGMT_002829"
     CDS             1340..1903
                     /gene="efp"
                     /locus_tag="ACHGMT_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFN6836511.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2004..2174
                     /locus_tag="ACHGMT_002830"
     CDS             2004..2174
                     /locus_tag="ACHGMT_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836512.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
BASE COUNT          742 a          384 c          419 g          644 t
ORIGIN      
        1 gtaaatgttt tttgataaat agcggtctgc tgcgtctgga aaaatcgtaa cgacacgatg
       61 accaggtggt aaacgacgaa tttctcttaa tgctgctgca aaagccgcac cactcgaact
      121 tccaactaat agtccttgtt ctcgtgctaa ctgcctagta tagttaaacc cttctacatc
      181 agagattgtc tcgatctgat tgattgaaag gggagaaaga aacggcggaa tgaattctac
      241 accgataccc tcaatttcgt gaggggctgg atcaccacca tttaacacag agccctctgg
      301 ttctacaccg attattcgaa tgtcgggata tttttctttc aaaaaggtag aagttccagc
      361 aaaggttcca ccactcccaa ttcctgctac aaaactatgg atattttctt tgatttcatg
      421 aaagatttct ggccctaacg tatgatagta agcagcagga ttatctttat tttcaaattg
      481 aagaggcaga tagctgtttg atatctcctc tgcaagtttt ttacttttgc tgatggcacc
      541 gatgattcct tgctcactag gagaatggat gatctttgct cccaatgctt tcatcaattg
      601 ttgtttttct aagctaaact tctctggaac aacaaaaacc gttcttaatt tgtatgtgag
      661 tgcggccaga gcgatcccaa ttcctgtatt tccggcagtt ggttcgatga tcgtcgtttt
      721 ttcattgatt tttccttggg caaatccttc tttgatcaaa taactgccca atctatcttt
      781 gatacttcct ccaggattta aatattctag ttttgcataa atagcagaac ctttaggcat
      841 agaatagtgc tgattgtcaa agcgataaag cggtgtggaa ccaatagctt ctgtaattgt
      901 cgagataatc atgtgggcct cctaaaaaaa gataataaaa aaaggagctg actattaaat
      961 cagctccttg aaagacatag agaaaaattg agtagataaa aagataagtt acctggtact
     1021 cattctatga agggcaatta atagacatga caaatagtat gctgctttta tgcaccatac
     1081 aacagcaacg attcaattgt ttttggttgc tgactgtcat gtgtatccct ccaagtaaaa
     1141 tttttattaa cgatagcatg aaaaaaaata aaagtcaaag gcttgaaaga aaaaaatatt
     1201 tcgatagcat cgtttacccg tgtttatttt tctgacaaat aggcatgaga ccttaaaaga
     1261 tagagaatac tctagttttc tggccggttg tctgatacaa taaagttgat tatgataaag
     1321 aaaagaagga gcaaaaataa tgatctcagc aagtgattta agagccggta tgactttcgt
     1381 acaagacggc aaactgatta aagttctgga tgcaagccac cataaacctg gtaaaggaaa
     1441 tacagttatg cgtatgaaac tgaaagatgt ccgttcaggt gcaacatacg acacaacgtt
     1501 cagaccagaa gaaaaatttg aaaaagcaca tatcgacaca aaacctgtac aatatttgta
     1561 cacgatggat gatacagctt tcttcatgga tttggaaaca tatgagcaat atgaaattcc
     1621 tgtagaaaca gtagaagaag aaatgaaata tatcctagaa aacatggaag taaaaatcca
     1681 atttttcggt agcgaagtca tcggcgttca attaccaaca actgtcgtat tacgtgttgt
     1741 ggaaacacaa ccttccatca aaggtgcaac agtcactggt tcaggtaagc cagcgacaat
     1801 ggaaactggc ttagtagtca atgttcctga cttcatcgaa acagatgaat tgttggaaat
     1861 caatacagca gaaggttctt atgtaaaacg tgcgtcaaaa taattcgcta acggattgat
     1921 aagaatttga aaacagagct tgacctcttg aaaggcagat catcggatct gcctttcaag
     1981 aggttttttg tggaatcagc aaattgaaaa ataaaaaagt atttgatttt atttatattt
     2041 tgaaagatga atggcaaaag ctagagaaag aaaaaactgc acttatttat gaagaaattg
     2101 aaatgattca taaaattgaa aatatccgaa ctattccgaa ctatattgaa gcgaaaatat
     2161 tttttcatca ctaaatagtg ttgaattta
//
LOCUS       DAZBSA010000194         2166 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000194 DAZBSA010000000
VERSION     DAZBSA010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.201"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            91..540
                     /locus_tag="ACHGMT_002831"
     CDS             91..540
                     /locus_tag="ACHGMT_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836513.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            540..1034
                     /locus_tag="ACHGMT_002832"
     CDS             540..1034
                     /locus_tag="ACHGMT_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836514.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            1048..1479
                     /locus_tag="ACHGMT_002833"
     CDS             1048..1479
                     /locus_tag="ACHGMT_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836515.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            1481..1618
                     /locus_tag="ACHGMT_002834"
     CDS             1481..1618
                     /locus_tag="ACHGMT_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFN6836516.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            1655..1948
                     /locus_tag="ACHGMT_002835"
     CDS             1655..1948
                     /locus_tag="ACHGMT_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836517.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            1945..>2166
                     /locus_tag="ACHGMT_002836"
     CDS             1945..>2166
                     /locus_tag="ACHGMT_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFN6836518.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
BASE COUNT          800 a          326 c          465 g          575 t
ORIGIN      
        1 aaattccaag aagctgtaaa tcaagcagaa aattacacaa acgaaagaat tccaaatttt
       61 acttttgaaa aaattgggga ggtatagaga atgacagata tcgtaaaaat aaaacaaagc
      121 aatgtacagg tttatccaca aactcattgg aatgctatag aaggcaaacc aacaacggtg
      181 aagggagata aaggcgatcc tggccaagca gcaacaatta ccataggaac cgtttctagt
      241 ggttcaaccg cttctgttac gaatgtagga acatcatctg cagctagatt taattttgtt
      301 ttaccaaagg gagataaagg tgatcctgga ataaatgcca caactacagc tgtagctaca
      361 actacagcaa atgggttgat gtcgtccaca gataaaacta agttggatgg aattgcagct
      421 ggagcacaga aaaatccagg taatgctaca actacgacag caggtttaat gtcagcaacc
      481 gataaagtga aacttgatgg attagcgaat attacatttg agaaagtggg gacggtttaa
      541 tggctgatat tgttcaatta aaagaagacg gagttgctaa atacttaaaa acgcatgtag
      601 aagctattga tggtaaagaa gcattggtgc agacagatgg agaccaagcc attgcaggac
      661 ataagaattt ttcaggttct gtaacaatca ataataaacg tgttttgacg actgatgata
      721 acagatatga agtggtaaat ctggttgtca ctaacggtaa tacagggaca gcaaagcttt
      781 atcgtgaagg aaaaacagtt tctatatatt ttgtggcttt aaacggaaaa agtagtggcg
      841 ggaatgattc ggttatttta actgttccag aaggctatcg gacaccaatt agttttgaac
      901 aactggttgg gtcaatagac cgttctactt tgaacagtgc tcaactatct attggcgcag
      961 acggagccat taaatggaga agaaattcaa gttatggatc agcttattca tttgttatca
     1021 cttattcaat ttaagggagg aaatctaatg aaagtagttt acaaatcaat caagccttac
     1081 ggattcgagc aaatcatttt gaacaatcaa gaaaatatcc ctgaaaactg tacagagatt
     1141 aaaccaccag ttcctaactg gagaccaaga tttgattttg ataaaaaaca gtgggttgag
     1201 ttagctactg aagaagagaa aaaagggaat gctgttgata gcgtagatga attggctaat
     1261 attaaggcat tatacgaaac gctgaaggca gaaaatgatg aattgaaaca gctaaattct
     1321 aaagcaatgc taaacaatgt agcaatcaag caagaaaatg tcttattgaa agaaaagtca
     1381 gaaagtttag cgcagttgaa ttcaaaaacg atgcttgctt ctgtacaaaa taccaaggaa
     1441 atcgaagaaa tcaaaaaaca attacaaggt gggaagtaac atgtattcat atgaagatat
     1501 caaactaatg tatgactggg gctgtttcac taacgaacaa gtaatggttt tcgttccatt
     1561 atgcattaca gacgaagaag cagataaaat cattaataaa gataagagcg catcttaatt
     1621 gatgcgtttt tatttaaggt aaaggagttg tcacatgatt aatttagggg aatggggaat
     1681 gatagcagga tcaataaccg ctatcgtttc tttgatttta ttagtaatta gaccgattgc
     1741 tgcatctttc tcgaaaatta ctgagactct ttcaaaagta agccacaatt tagatttgct
     1801 gactaaagat ttagaatcga gcaaatcaga tcgattgatg attcatgaag aactaaagaa
     1861 acacgatgaa agattagata cacatacaga aaaattggtg gaacacacgc aacaaatcaa
     1921 aactttgttt agggagagaa gaaaatgaat aataaaacgt tcgaagtact aaaatggttc
     1981 gcactggtaa ttattcccgc actagctact ttcgtggggt tagttggtaa agcgctcaat
     2041 tggcagtaca cagatatctg tgttgtcatc attactggtt ttggcgcgtt tttagggagt
     2101 gtgttgggtg tatcaaatcg aacctacaaa atgttctcgg ctgaaagcga agaaggagga
     2161 aacaaa
//
LOCUS       DAZBSA010000195         2071 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000195 DAZBSA010000000
VERSION     DAZBSA010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.202"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..442
                     /gene="dhaM"
                     /locus_tag="ACHGMT_002837"
     CDS             47..442
                     /gene="dhaM"
                     /locus_tag="ACHGMT_002837"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFN6836519.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            417..1427
                     /gene="dhaK"
                     /locus_tag="ACHGMT_002838"
     CDS             417..1427
                     /gene="dhaK"
                     /locus_tag="ACHGMT_002838"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFN6836520.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            1455..2060
                     /gene="dhaL"
                     /locus_tag="ACHGMT_002839"
     CDS             1455..2060
                     /gene="dhaL"
                     /locus_tag="ACHGMT_002839"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFN6836521.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
BASE COUNT          690 a          351 c          526 g          504 t
ORIGIN      
        1 agcgaaacca atcgttagca actatgtaaa agaaaggaag attcttgtga aaaaaagtat
       61 tttgcttatt tctcatagtc aaaaagtaac agatggtatt aaagaaatga ttgagcagat
      121 gcaaaaatcc gaagaggtgc aaattttttc tctcggcgga ataaatggcg agatcggatc
      181 agacccgatg aaaatcgtag aagcagtcaa tgaatcaagc gatgcagcta gttattttgt
      241 ttttgcggat atcggtagcg cggtattgaa ctcggaactg gcaaaagaca tgttggaaga
      301 agagcagcag caacgttatc atctggtgga tgcgccttta gtggaaggag cttttgcagc
      361 agccatcact gccggtataa cagatgatac tcagcaaatc ttaacagaag cacaaaatgc
      421 cggaaagaaa ggatgggaat aaattgaaaa aaatcatgaa tgatccaagc aatattgtcg
      481 aagaaatgct ggaaggatta gtaaaaagct atccagaact tgttcatcgt gtagaatctt
      541 cccgtgttgt agccaagaat cagaaagcgg aacaagttgg tttggtttct ggtggaggaa
      601 gcggacatga accatcccat gccgggtttg taggggaagg gatgttaagt gcagcggtgc
      661 ttggtgatgt gtttacttct ccaactccag atcagattca gacggctatt aaggaagccg
      721 atagtggaaa aggagttttg ctgatcgtta agaattatac aggggatgca ttgaacttcg
      781 atatggcaaa ggaattagct gctatggacg atattgaagt agaaagtgtg atcgtagatg
      841 atgatatcgc cgttgaaaat agtacgtata ctgctggaaa acgcggagtt gcaggtactg
      901 tccttgtaca caaaatcgtg ggcgatgcag caagaaacgg tgcttcccta gctgagttga
      961 aagaactggg agaaaaggtc gtacaggcaa ctaagacgat cggtctagcg cttcgagcag
     1021 caactgtacc agaagtaggg aaaccaggat tcgaactagg agaagatgag atcgagtatg
     1081 gtgttggtat ccacggtgag ccaggctatc gtcgagaaaa gatgcagcct tctaaagtac
     1141 tggcaaagga acttgttact aagattttag atgattactc gaatttacca aaagaagcag
     1201 gtgtactagt caacggaatg ggcgggacgc ctttgatgga acagttcgtg ttcatgaatg
     1261 atgttttagc tctgttggaa gaaagaggcg tcaatgtagt tttccgaaaa gtcggtaatt
     1321 ttatgacctc actagatatg caaggtcttt ctctaacact gatcgacttg acggagacac
     1381 aatggaaaga ttctttagaa agcaatgtac agacgatttc atggtaaaaa atctcaagat
     1441 aaaagggtga taatatgaaa ctaaccgttc aagaaatcca agaatggctc acgcaatttg
     1501 cagaggcaat caatcagaac aaacaatatc tcagtgatct agataccccg attggcgatg
     1561 gagaccatgg aaataacatg ggaagagggg tttcagctta tgaagaagct tttcaagccg
     1621 atcatcctga aacaatcagc gatacattca aagtattctc tatggcgatg atatcgaaag
     1681 tcggtggggc ttctggtccg ttatacggat ctgcattcat gaacatgatg aaagcgacaa
     1741 aagaggtcga ttctattgat tctcaagaaa aattaggcga agtgatcgag cagggaacag
     1801 cgggtattca aagccgagga aaggcagaag ctgacgataa aacgatgtta gatgtttggc
     1861 tgccagtcac agaggcattg aaggcaggga atttaacaaa agaaatcatt gaccaggcaa
     1921 aagaacacac caaagtcttg gtagctaaaa aaggacgagc ttcctatcta ggagagcgtg
     1981 caatcggaca tatcgaccca ggagctgctt ctagtgccat tttattttca acgcttttag
     2041 atgtgatcaa tcaaccttaa gttcaataaa a
//
LOCUS       DAZBSA010000196         2001 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000196 DAZBSA010000000
VERSION     DAZBSA010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.203"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            614..946
                     /gene="traE"
                     /locus_tag="ACHGMT_002840"
     CDS             614..946
                     /gene="traE"
                     /locus_tag="ACHGMT_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFN6836522.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1565..1906
                     /locus_tag="ACHGMT_002841"
     CDS             1565..1906
                     /locus_tag="ACHGMT_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFN6836523.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          691 a          269 c          423 g          618 t
ORIGIN      
        1 atgtttgcat actccttttt tttgagttat aataatacgc gaagagagat aatttcatta
       61 tttaaacaag ctaaaaatct tgatattact tcttttcagg caatacgaat attattcgct
      121 tacatttttt gtataaattt aattgtctgc tatgcttgtg atgaattgat tttcgttttg
      181 cacttttgga tgataaattt ggacaattga aaatacaaaa aaaagaaagg cggtatgaac
      241 tcggaaggct ggtaaccttc gtcagcgttc atacacggca ttaagatttg gcccttaatg
      301 cgttaacctt tcagtgttac ggtattaccc gcaatggatt tagtataaca gatattcaaa
      361 agtgatacca ctttttttgg ctgtgttaag gttaaaaata cgtgatatta cgatgaatct
      421 atgcctaagc ataatgttat aaaaatgtcc gtgtggctct ttgccgtatg acgaacgctg
      481 aaattagagg gaacgcatgt ttggatgcgt tccctctttt ttgtttgtca tgaacgaaaa
      541 tcaattctct atttttgaaa aataacaaga aaaacttgtt aaaaatattt aaagaaattg
      601 ggtggtttat tttatgtcat atacaggaat tttgagtctc aaagatattt gtcactacgg
      661 taaacggtgt accgctacag agaaaatcac gaagaaacta tcaactggcc aaagtaaaac
      721 cgttgtgcag tgtaagaaat atataatcca gaaagacaaa gtctcagagg aaatgattta
      781 ttacattggg aaacaaaagc aaataatttt aaaagatcct atccccttaa aagagctata
      841 tccaacaatc aaacatgttt atgaccaaaa cggtgtactg attggtcgaa gaaaaaatgg
      901 agtcttgcgt tgtaccgcaa aagggatggg gcgtttgatc ggctgaattg atcattctca
      961 taacttgtat tttatcgttt ttcgcttgct tgaaaatatt gtcgagactt ctaaaggaac
     1021 ttagagtcac tagaaaaata aggagtagac ttgatctatc aacaattccc ttcggggagt
     1081 gacggggtag gttttaacga ttggtaacgt aaaaaattgg tcaatcgagt aaatgcgctt
     1141 gtttggatag tggtgaaatg aatttgtgta aataataagc gcagagtaaa caagagggaa
     1201 tttagaagtt agacaagtta gaatagcgtc tagattaatc atgagcagct actaagggat
     1261 ctagactttg acgcaaactc atggaataag gagtaggtct tctcgtaatg gggaggacgc
     1321 acaagaaatt gtgtcgtgtg ctgcagtaca tgtagatact tgcgaggacg ttgattattg
     1381 gatcaatcga gcatttaccg ctgtctacta gggtttctcg tttcggacgc cataaacgag
     1441 tcgaaatttt ttatgtattt ttaagtaaac gaaaaacgat aaaatatgaa caagtcctga
     1501 ataaagtgaa aaaattgata taaaacatag agaaaatgaa atttacgtaa tggaggaatg
     1561 acaaatgaaa aattatgatc cgaatatacg attgggcaca catactatta aggtttcttt
     1621 ccaacgttgg gattacaaag gatttgtgac gtttagaagg ggcggaaatt gtaaaggatt
     1681 agatgtttta gcattagacg aggatgattt atacgatcaa acattgactg ataatccaat
     1741 tggattcggc ttgctacctg aagatgatga aggtaatgaa tggtttaaaa tgacgcttat
     1801 gaatgataat ggagacgaat tatctgttga agatacttgg tcatatttga gtgattatat
     1861 tgtcagcttt gaaatcattg aatttgtagc ggataaagaa gaataaaaac tggaggaata
     1921 aattttacag ataaaggctt agaaggagtc acgacgatta ataaaaaggt agtattacct
     1981 taggcattga gtaaggaatt t
//
LOCUS       DAZBSA010000197         1988 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000197 DAZBSA010000000
VERSION     DAZBSA010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.204"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..426)
                     /locus_tag="ACHGMT_002842"
     CDS             complement(<1..426)
                     /locus_tag="ACHGMT_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFN6836524.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(535..1566)
                     /locus_tag="ACHGMT_002843"
     CDS             complement(535..1566)
                     /locus_tag="ACHGMT_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN6836525.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          613 a          327 c          343 g          705 t
ORIGIN      
        1 aggaatctgt tttgattttg ttccatttga aatttctctc actcgctttt ttgtctcatc
       61 atcaaataaa atgaaagggt cttctaaacg atcgataaat acttccattg ttgttgttgg
      121 tagatgataa gaagtcatga caggcactgc accaaggtaa gacacagaaa cagctagaag
      181 gtatgtgtca aaagcggaac tcttataaat aataactttg tctccggatt tcactccaag
      241 atgagccaat tgataagccc gtttcaacac actttcgcag cattttttgt aagttgtttc
      301 aagtcctaat tctgggaaag ctgaaagtgg ctcatcaaag ataatcgggg tctcggaaaa
      361 gttagctgca gcttcttgaa aattcgtaaa taagttcaaa ggggaatacg ttatggtctc
      421 catcattcac actccattat cttttgttaa gtaatataca aattcaagat aatcgtaaca
      481 taatccttta tataataaca tagtttattg tgaaaaaaag atcatacatt tgttttatat
      541 ggaagaaacc cgaacgaatg ccgctaattc gtgataagaa aaatcaacca tcttttccca
      601 gatatcttga aaagaatggg atgagaggta tcgattacca cataaataat ttaccgcgtc
      661 agcatcatct aaatatagtt ctgcataata acgatatgtt tttctgcata gttctggcga
      721 tacgttttta tatactgtaa ttttgctcaa aatttggcga atagtccgag gagaaatttg
      781 ctgatcatgt gaggaacgaa aaacaaaaga attttcgtcc gtattttgtg ctgttaactt
      841 aaaatagttc tccagaaatg gcatcgcttc ttttggtatt ggtactttat tcagtctgtt
      901 ttcttttcta ttcagattaa gaatctgctt ggtcaaagat acatctttac gtttaagttc
      961 tactacttca tggatcttta atccgcaata cagaagaatg gcagtaattg caaggttcct
     1021 cggtctattc ttttcttcaa agctagcttc tctgcttgtt tgaaagcctt caatcgggat
     1081 aaacaaatgc tcaaaatctt tgaaaagttc tgcatcgata tatcgcggag ctgctttggt
     1141 tgtttccggt aaacttgtac ccaaaagaga aatctcttct tcaatcagtc ctttttgata
     1201 caaaaatcgt aaaaaatgat ttaatgaata ttgcttcctt gaaatcccgt aatagctatt
     1261 ttcaaacaat tgctccgttt cttggttcgc tgtagtttgg aacgaacgag tatgtcgata
     1321 gattttagct aagaaattct gataatcttg tttttctaaa tggataatca gctgatcatc
     1381 cacttcttca gaaaatactt ttctcaaata atcagtaaac agatgaaaat cttttgcgta
     1441 ttcatagcga ctgcctaatg aaagggaaga acgattttct tctaaaaact gttgaaagac
     1501 ggaagggagc aagtgtgtac gttgctttaa cttttcttgc agttgttttc tagttttgct
     1561 gtccattaca aattccctcc aatagtgttc attttctata ttttaccgca tttcatcaaa
     1621 tgaagataga cagtttgtct tgatcttagg aagcaaattt ttaactaagt actagctaaa
     1681 agatatgaag taagaagata catttttagt tattttatta gtgcagtcat tcttttgatc
     1741 ttttaattga tttatcctct ttttagctat gtggttactt catgagcaat gaaatggatt
     1801 tggttcgttc taatggcagg aggctctttc ttttatttcc gaaaaattag aaaacatttt
     1861 ttcagaatcg attggctctc catttattca ataatggttt gtctaacgat attgttggga
     1921 taattcttaa agaagtatta gaaatagttg gtacttcatt actttacatg aagatttatt
     1981 ctatggta
//
LOCUS       DAZBSA010000198         1910 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000198 DAZBSA010000000
VERSION     DAZBSA010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.205"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..>1910
                     /locus_tag="ACHGMT_002844"
     CDS             73..>1910
                     /locus_tag="ACHGMT_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFN6836526.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT          716 a          232 c          383 g          579 t
ORIGIN      
        1 tggaaaaaat gaaattaaat ggacgaaaaa tattcattca tttgaaataa tgcctaggtg
       61 gtggagaaaa gtatgaaacc aagaatatat agtcctactg aaacagattt ctcaacgaat
      121 ggcttaggaa ttttaaagga ttgtacaaga tgtgaaatat atgaagtagc taatggaaaa
      181 tatgaattag agttggatta tcctttagga actagatttg atgaatattt tgaaaatgac
      241 tatcaaataa aagcaaagcc aaacgatcaa gaagagtatc atatcttttt tattgatgat
      301 aaagatatag atactttttt aaatacagta actatttatg ctcagagtcg tacaaatcga
      361 cttggaagac gggtagtcac tcttgtggaa gtggactcta aaactggtca agaagcgata
      421 tcaattattg aaactaaaat ggataaaaaa tctgacatac gactttattc tgatattacg
      481 gctgtttcta gcacaatctt tgaagcgaga aatgttttaa attgtattgc tggtgaacaa
      541 ggatcattgc ttcaatattg gggcggagaa attaaacgtg aaccatttaa gctttctttg
      601 ttaaagcgaa gaggtcgcaa taatgttgga acgattcggt atggaaaaga tatgtctggt
      661 ttgaaggtca aattagattg gacaggtata aaaacaagaa ttattccata tgctgatcct
      721 caaagtgaag taggtacgac taatcgaatt tatggttcgc cagtagatag tgcctatatt
      781 aataactatc ctgatgtcta tacagaacat gttcagttta cagaagaaca aggagtaaaa
      841 gacattaaga gcttaaataa aatagctaaa aattacttta aaacgatcaa tcctggctgt
      901 gataagccta aagtttctat tacggttgaa tttgataagt tgactgatag tgaagaagcg
      961 aaagaatttg cgaagattag aaattatggt ctgtttgata catttaaaat atatcataaa
     1021 aaatataata tttatcttga atcaaaggtt agtggactac aatacgattc tttatctgaa
     1081 aaaactttga aattagaagc aggagacatt caagttgctt tttatcagca acaagctgta
     1141 actattcagg ataaattaaa agattatgca acgaataatt atatgagtga ttttaatgat
     1201 tatgtttctt caatgattac aggtcaaggc agtgcaggag gatatgtagt tttatggcca
     1261 aaagaaaaac cttccaacat ctttattatg gataatccag atttagaaaa ggcaagagaa
     1321 gtactaagaa tgaataaaaa tgggattgct ttttctaaga aaggatggcg ggggcctttt
     1381 aattcagctt ggacattaga tagtatattt aatgccaatt ttatacaaac tggattaatc
     1441 aaagcagata tctttcaaaa ttcttttaac aagactggag atgtattgaa attagttaat
     1501 ggactactcc aaatttggaa caacaaaaag aaaataatgg agttaactaa aaaaggaatg
     1561 gaattttgga attctaaggg ttcaattgga acgattggaa caactgattc tgttggtaat
     1621 ccttttcctg gggcttctac tccaacccct attgaagata attctttagt tattcgtaca
     1681 aatggagacg gcaaatatat tttgatttct cctaaagtag gaaaaggatt agttttatta
     1741 ggaaatggta aagcaattta ttttggagac ttagatgtac aaggaaaact tacagttaaa
     1801 ggaaaagaaa taattcctgg acaaggtggt ggtccatcgg gaggtggaga atctactggt
     1861 gggtatccta gtgaattaaa aacagatgca gaaaagagag cttggagaat
//
LOCUS       DAZBSA010000199         1877 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000199 DAZBSA010000000
VERSION     DAZBSA010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.206"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(98..496)
                     /locus_tag="ACHGMT_002845"
     CDS             complement(98..496)
                     /locus_tag="ACHGMT_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFN6836527.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(493..1266)
                     /locus_tag="ACHGMT_002846"
     CDS             complement(493..1266)
                     /locus_tag="ACHGMT_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFN6836528.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1263..>1877)
                     /locus_tag="ACHGMT_002847"
     CDS             complement(1263..>1877)
                     /locus_tag="ACHGMT_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFN6836529.1"
                     /translation="FVFAPLEGEDLASFLVGAAIMMIGMTLFLFGADYSMMEVGDLVG
                     KYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQQVYDISDGEIGRVLLIGIVSVGT
                     GVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFTSSEFMPIAFDSGGVTTGPVTVPF
                     ILALAGGMTSMIKQKKNENDSFGMVGIASLGPILAVMILGVIFQ"
BASE COUNT          588 a          472 c          291 g          526 t
ORIGIN      
        1 tatttcataa gtattcacga gaaaaataca aacaaaaaag cggaaataac gctcatccgc
       61 cttcttgttt gtgtttattt cctcttcaaa cgcttgtcta tgattcagac ttttctctat
      121 cgttattttc atctaaaagt tcttgcattc cgataatttc tcgatattga tgaatcttag
      181 tgataccaat gactttgtct acatcaatac taaaacaaat accattacct ggaatatcga
      241 tgttgatgcc ttgaccaatc gcatccatta ctttattcgt caaactatcg ggtactaatg
      301 tcaacacaat atctttttct ggcgaaatgt ccaaacctaa aaatttccct gaatcatgta
      361 caccagaacc tcttccatgt aagatcgttc cgcctgctgc acctgcttct tttgaaaagt
      421 cgataacatc ttcgcccatt cctccgtcaa cgatcgtcac aatcatgtga agattcaatg
      481 ctacaagatc tttcatttgc tttcccccaa tttttgcaat ctttcttttt cgattctgtc
      541 tttttcatct tttcgtttat aaatatatcc tagtactaaa gtggataata ttggtgtcaa
      601 tgcaatcatt gcaaccacac caaacccgtc aatgatcgga tctcttcctt caatttgtgc
      661 agcgattgcc acgctcattg ataaaataaa tgtcgaacac atcgggcccg ttgctactcc
      721 accgttatca aaagccattg ctaaaaagat cttgtccact tttcgtccta aaataaatgc
      781 tagcccgtat cctgggatca agaaataata aagagagaat ccgacgagca aacgccacat
      841 agacaatcct actgctgcgc cgatcccaat tgaaattacc atcagcatga ttttcgatcg
      901 gattctcccc tcgctcaatt cttcaacctg tttgatcatt acatgaatcg ccggctcagc
      961 aaaccctact aagaacccca ttaaaaagcc aaggggaacc agtatataat tgtaaggcaa
     1021 tcccgcaata gcactgccta aatgctgtcc taccggtaca taagccatat tgactccgtg
     1081 caggaaaagg atcaacccaa tagtcgttat caagaaacct ttcataatgt caaagaaacg
     1141 ctttttgcgc atacgaaaac taatgatatt cattaaaaca aataggatta cgataggtgc
     1201 cactgccaaa atgacttcca tggtaacgat ttcgattcca tctatcactt gctgccagaa
     1261 actcattgga agatcacccc caaaatcatc acggcaagaa tcggccctaa cgaagcaata
     1321 ccgaccattc caaaactgtc attttcattt ttcttttgtt tgatcatact agtcatccct
     1381 ccggcaaggg ccaagataaa tggaacagtt actggaccag tagtcactcc ccccgaatca
     1441 aaagcaatcg gcataaattc ggaactggtg aaaaatgagg cgatcaaaac gcctatgtag
     1501 cctatagcca tcaattgata ataggatagc ttgaatacga cccgaagcag cgcaaaggct
     1561 agaaatactc cagtgccgac gctgacgatc ccaatcagca acactctgcc aatctctccg
     1621 tcagatatat catatacttg ttgtcccaaa acttgcacgg aaggttcagc aatcgtgatc
     1681 acgatcccga tagcaaatcc caaactgacc aaaacggtca aacttttctt tttgatcata
     1741 tatttcccta ctagatctcc gacttccatc atagaataat ctgctccaaa caaaaagagc
     1801 gtcattccga tcatcatgat tgccgcacca accagaaatg aagcgaggtc ttctccttct
     1861 aacggtgcaa aaacaaa
//
LOCUS       DAZBSA010000200         1859 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000200 DAZBSA010000000
VERSION     DAZBSA010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.207"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            416..1804
                     /gene="ltrA"
                     /locus_tag="ACHGMT_002848"
     CDS             416..1804
                     /gene="ltrA"
                     /locus_tag="ACHGMT_002848"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFN6836530.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
BASE COUNT          654 a          343 c          444 g          418 t
ORIGIN      
        1 aacttggtgg tgaaagtcca ctacaggctt ggcagtagga actgttagcg aaagacaagg
       61 cggcaatcgt gaggttgtgg ctgaaggaag tcggacgcaa actcctgaac tgacgaacag
      121 aaactagata caaggctgaa ttaggtcgga taagtcttca tcacaagaca aagtccaata
      181 ctgcccgaaa cctatacagt aaatctagca gttacatggg aggaaagttt tcactcttac
      241 cgggggagat ctcatgaggg tgttgtgggc gttgaatgaa aacaaaccag cacaacgatc
      301 atggcgacat gatggtgaat catgagaagt cagccgaggt catagtagta acgacactga
      361 ccgttacgaa ggactgaaca ataagaatct tgaataattt aggaggtgtg agaagatgcg
      421 acgaccgcag aaaacaagaa aacttggcta tcatcaaaga gctagtgtgg aacacgaagg
      481 gtatgatgaa gtgcgcagca cttcttgcgg tgaacagagg aaacaagatg gtgtagaaaa
      541 tctgtttggg agaattctag atcggaataa tctcaatcaa gcgtatcttc aagtagtcag
      601 aaataaaggg gcggctggtg cagatgggat gaccgtcgat caactgcttc cttacttgaa
      661 agagcaccgc gaggaattac tatccaattt acgccaaggg aactataaac ctcagcctgt
      721 tttgcgagta gaaataccga aaccaaacgg cggtgtaaga aaactaggaa ttcccacggt
      781 tattgatcgt atgcttcaac aggcaatcaa tcaagtgtta caaccgctat ttgaaccaga
      841 attctcagat aatagttatg ggtttcgccc aaatcgcagc gcacatgacg cagtgaagaa
      901 agcaaaatct tattatgaac aaggttaccg atatgtggtg gacattgata tgaaagccta
      961 ctttgacacc gtaaatcacg ataagctgat gtatttcatt gagcggaaag taaaggacaa
     1021 acaagtccta aaactgatcc gtcaatactt aatgagtggt gtaatggaaa atggactcat
     1081 cacaacaact gaagaaggaa cgccccaagg aggcaatctc tctcccctgt taagtaacat
     1141 ctacctgaac gaatttgatc aagtgctaga aaatctagga cataaatttg ttcgatacgc
     1201 cgatgactgc aatatctatg ttagaagcaa acgagcagga cagcgtgtga tgaagaaatc
     1261 cattcaattt ctcgaagaac aactaaagtt gacggtaaat cgagaaaaga gtgcggtagg
     1321 aagtccattg aaacgaaagt ttcttggatt ctgcattctg ccaacaaaga aaggtattaa
     1381 aattcgccct cacgcaaaag caaaacagcg tgtgaaaagc aagctcaaac aactcactaa
     1441 aagaaaccgt ggaagaagta tccaacttat tttaaaagaa atcaagcaac taatgactgg
     1501 ttggatcaac tactatggaa ttggtgagat gaaagaattc atgagggagt taaacggttg
     1561 gttgaagcga agaatcagac aatatatttg gaaacaatgg aagaatccaa gaaccaaaag
     1621 aaaaaatctg attcgtttag ggatcgataa agcgaaagcg tatgaatggt caaatacaag
     1681 aaaaggtatt tggtcagtct ccaaaagtca tattcttcat cgatcattaa cagacaaaga
     1741 actggcccgc caaggatatg aggacatctc cttgaagtac cagttcgtac actcaactta
     1801 ttgaaccgcc gtatacgggt ccgtacgtac ggtggtgtga gaggggcgga aggtcagtt
//
LOCUS       DAZBSA010000201         1824 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000201 DAZBSA010000000
VERSION     DAZBSA010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.208"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            177..599
                     /locus_tag="ACHGMT_002849"
     CDS             177..599
                     /locus_tag="ACHGMT_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836531.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            609..812
                     /locus_tag="ACHGMT_002850"
     CDS             609..812
                     /locus_tag="ACHGMT_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836532.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            1023..1607
                     /locus_tag="ACHGMT_002851"
     CDS             1023..1607
                     /locus_tag="ACHGMT_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN6836533.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          642 a          253 c          323 g          606 t
ORIGIN      
        1 gtgtcacttt tctttttaaa tggcaatatt ttttgtaaat aaatgtcaat caagaaaatt
       61 taaacgatta tgacattaaa aatatatttt atgaattttc tagtatataa cttgatttat
      121 ctgttaagat ttattaagaa aacgttttct tatttttgat ttcgaggtga tagacaatga
      181 aaaataagtt tattttatct ttaggtattt taactattgg aattagtgta actggaacta
      241 caattcctac atttgctgat agtacaaatg aaccaactac tgtagtagat aataaccaag
      301 aattaactcc tttagatagg aacggaatag ccattggtaa cccaactaac gacttaactg
      361 ttactgaaaa ttatttaata gtttctccat tacttgctcg agcagcttct aaaacgatac
      421 gagtgacagg aacgatgatt tttgataaag ttggttggcc ttctagacct cctcaatcac
      481 ggaattggaa agaaaaaaga aatggttatt ggtactatgg aaaattgtat ttacaaagtt
      541 ataaatcagc tggaaacaat tggattgcga cttattcagg aaccttaact agaaagtagg
      601 gattttttat gccagaattt gtgaacacac caaattttta tcaagatagc gatattctaa
      661 gtaaaattaa tttaccgaaa gaagaatcag ctttacaagt tcagcgaact ttacaggaga
      721 cacaaaaaat tcaaaaatca atttctaatc tagaagattc tgaagaaaaa agaaaaaaaa
      781 gagaccaaaa aacatatcaa aaagaaaaat aatgatttta aaaaaagagt gtctagtcga
      841 cattcttttt ttctaattga aagtgttcat aaaatcaccc atttctggac actttctttt
      901 tttgctttat ttttcaaaaa aacaattcat ttcatgttca taaacccata acaatatcta
      961 tgttcagtta aactatatgg ataaagttat gttacaataa aattaatgaa ggagtgattt
     1021 ttttgaaaat aggatacgcc cgggtatcta ctggtttaca aaatttggat ttacaagaag
     1081 acagcctaaa taaatttggt tgtgaaaaaa tttttactga tcatatgagt ggggcaaaaa
     1141 gtaatcgacc aggattagaa atggccattg attttgttcg ctctggcgat acacttgtcg
     1201 tttggcgctt agatcgttta ggtagaaata tggaagattt gatttcaatc gtcaatcgac
     1261 tgaatgagcg tggtgtgagt tttcatagta ttcaagaaaa tattacgatg gacaaatcaa
     1321 gttctacagg acagctcatg tttcatttgt ttgcggcatt tgcagaattt gagcgtaatt
     1381 tgatcctgga acgatccgca gcaggacgag cagctgctcg tgcaagagga cggtttggtg
     1441 gtcgtcctga aaaattgtct gaacaagatt tggaattact gaagacttta gtggatagcg
     1501 gtacacctat taagactatt gcggaacggt ggaacgtctc aagaaccacc atttatcgtt
     1561 atttggcaaa aatggaggag aaaaatggaa agacaaattc tcattagttt ttgtcattta
     1621 atgctgaaaa tttttgccat ttatctagaa aagtgacaat agtatgagaa ttttttgtaa
     1681 gtgtctactg ataacttatt ctcaaatgtt gtaaggattt tattcatttg ttaatattaa
     1741 agaacggaac tccaaaaaag tccgtactct aatttgcagt ctctcactct tttagagtga
     1801 gagtttttat tagattttca aatt
//
LOCUS       DAZBSA010000202         1753 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000202 DAZBSA010000000
VERSION     DAZBSA010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.209"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(209..1675)
                     /locus_tag="ACHGMT_002852"
     CDS             complement(209..1675)
                     /locus_tag="ACHGMT_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFN6836534.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT          554 a          357 c          306 g          536 t
ORIGIN      
        1 caaaagtcgt gtcaaagtcc cttgtttcga ataaatggtg ttcaatcagc tgaccttcag
       61 cattagctca aaaaacggca aaatatgagg agctattttt gtttggctgt tcttattact
      121 tgatgcagga cagctttttc acaacctctg attcacggtg ttccagaagt agcttctgtc
      181 acaacctctt gtaacaagta tttctatttt aaggctgaac cactgtttct ggttcagtaa
      241 tttctttgtt tttgtctcga cgagcaattg ctggcaagat catacctaat aagatcaaga
      301 caattggtgt gatgatattc gtgaataatt ggaaccacca tgctttagga tcagcagtat
      361 aatctacttt tggcaccatt cctaggatac atgcaaacgc agtgaaggca aagcaccaag
      421 caccaaagat aaaaccgatt tttggatttt taacaaattt atattcagaa ttgaatttct
      481 tataagcttt attcaacatc atatatgcca agaacaccca taggtaacgc attggcatga
      541 caactgagtt caagttcgtc aaccatttta ctaattcatt catatctttg ataccaaaga
      601 tcggcaacaa aataatgatt cctactagta tacctgtcag aagataacca ttcaccaatg
      661 tacctttacg attacgttta cgtagccagt ttggtacaaa ttctggatcc gcatctgcta
      721 acaagatttt tagtggtgca tcaattgaaa aggctaatgc ggagatttgt ccaatcatgt
      781 ttgtcaatgc gtaaatgatc atcaatgagt taccgatacc atagtaatta cctaaaattt
      841 gaaaagcact gtatgcccca tttgccatca agtctttagg gatattatcg ctagcaaaca
      901 gcatacccat tgcgactgag cctaatacag cacaaatccc taccattgct gctaagaaga
      961 tcatcccttt aggaaattcc tttgcaggat ttcttgtaga gttgacataa ggggatattt
     1021 tttccgcccc tccaactgca aatactagca tcgaaacggt tgtgaaataa ctgaaatcaa
     1081 atgttggaat atatgttttc acttgattca tatgtggtgt ggcaaattcc atcgttggat
     1141 tgatgaacgg tgccgttaca gctaaaagaa taaataagat cgacatgacg aacatcgctg
     1201 ttccggccag tccgccaatc actttcaaag tagaaagtcc ttttgttgat aaatataaaa
     1261 aggcaagaaa tataataagt gaaataactg ctaccatttg tactgacatc gtatttacga
     1321 tatttccatt tccttggact gcccaaccac cagcaatcag aattgcttga ggcttttgtg
     1381 ccaaataagg aatatggacg acccagtaag tccaagcggc atagtatgcc agtcgtttat
     1441 tagatgtcgt attttcgacc catgaactga cgcctccttt actatccttg aaggctgatc
     1501 ctaattgtcc aacgatcaat gcgtatggaa taaaataaat ggccaaaatc aatagccaag
     1561 aggtgacaac tgatactcct tgctgagcat agttattaac tacattcccc atgccccaaa
     1621 ccatgttaaa tgcaattaac gcaacagtaa accagcgtaa ttgtttgttg ttcattcgtt
     1681 ctctccttta tgataaaaat ttgttatcca gatgtaatca tattcaattt ttaacaaact
     1741 tacaagacag tgt
//
LOCUS       DAZBSA010000203         1723 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000203 DAZBSA010000000
VERSION     DAZBSA010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.210"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            221..418
                     /locus_tag="ACHGMT_002853"
     CDS             221..418
                     /locus_tag="ACHGMT_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836535.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            524..1006
                     /locus_tag="ACHGMT_002854"
     CDS             524..1006
                     /locus_tag="ACHGMT_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836536.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1064..1660)
                     /locus_tag="ACHGMT_002855"
     CDS             complement(1064..1660)
                     /locus_tag="ACHGMT_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFN6836537.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT          526 a          258 c          351 g          588 t
ORIGIN      
        1 ttaatatgta ggaaataatt agtttttaag gaatatgttt taattttttg aaaaaaattc
       61 ttctaaatac aacagctgtt ttaattgtga aaacgaaaac agtacgctat aatgcctgta
      121 aggttgattt ttaactgtaa ataaaaatca atgagttaaa tctccaaaga aatagcagtg
      181 gtaatgtgga gaaaagttcc agcagtttag gaggattttg atgacggaag aaatgattaa
      241 tttaggagaa caatatgcat gcaaaccaat tggttttaca aaaacggtca ttggtgaagt
      301 tgtatcaaaa atgacaaact gcgccgtggt caaagttgcc caatgtgctg cagaggacca
      361 agaattatta gatgaaaaag caagcatggt tgttgcaaaa tatgatacat ttgaataaag
      421 aaaacaattg gacgtgcggc gtgctgcaag tccaatttct ttgagaaggt aaatcagtat
      481 tcttttccta ttttctggag aagaaaaagc gaggcgagtt gtcatggtaa cattctttac
      541 ggttctattg tttttattcg ttgttgtgat gtttttattt ctttggctgg ttcgaaaaga
      601 aattatttat cggactgtgc gaaatcgatg ggtctacttg gtgataccct tcttagtgtc
      661 actagtcatt tggtatacac tcatcagtca tcctacagca gatgaattgg caaaagggat
      721 cttgtcagca atgattttta ttagtttttt gttggacagt agaggaatca cagaagaagg
      781 acttgttttg aatagctttg acaaaaaagg tgttcctttt tctgagatca ataaaattgt
      841 attatatcag ccaaaaggtt ctaaaatcgt aaaaatgaat tttttccgaa acggatggcg
      901 aggaccgatg ctgaaatttt ctgcatcatt agaagaatta gttccatttt tgtcccaacg
      961 cttaaacgat gaggcagaga ttgatattat gattgatcca gaataaaaac agaggttgcg
     1021 acaagatgtt tgtcacaacc tctgtttttt atttcaaata aaattatcca aatacgcgga
     1081 agcgatcggc atcgttcatg tattcttttt cctcagccgg tgtttctggt gaaccgaata
     1141 ctaattgcga acgtagtttc cagtttgaag ggatattcca ttcttttgca actgcttcgt
     1201 caataactgg gttgtaatgt tgcaagtttg cgcccaatcc tttatcaact aatgctaccc
     1261 aagtattagc tgttgcgatt ccgtttgatt gttctgacca atctgggaag ttgtctgcat
     1321 ataattcaaa ttgttcttgt agacctttta ccacgtcagt atctttgaag aataatactg
     1381 taccataacc atttttgaaa ccagctaatt tgttttgtgt atttgggaaa gcttctgctg
     1441 gagtcaatgg gcgaagtgct tcttcagtga tttcccaaag tttttcatgt gcatctccga
     1501 aaaggataac tgcgcgtgtt gattgagcat tgaaagcagt tgggctttct ttaattgctt
     1561 ctttgatcaa agttgttaat tcttcattag aaagtgaaac attacgtcct agatgataga
     1621 ttgaacggcg gtttttcaaa gtgtcagtaa aagttgtcat aataaaattt ctcctttgat
     1681 ttgcgttttg cattttttta attgattacg aaatgaagta tag
//
LOCUS       DAZBSA010000204         1701 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000204 DAZBSA010000000
VERSION     DAZBSA010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.211"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            330..1175
                     /locus_tag="ACHGMT_002856"
     CDS             330..1175
                     /locus_tag="ACHGMT_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN6836538.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..>1701
                     /locus_tag="ACHGMT_002857"
     CDS             1456..>1701
                     /locus_tag="ACHGMT_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFN6836539.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEK"
BASE COUNT          648 a          206 c          282 g          565 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa g
//
LOCUS       DAZBSA010000205         1687 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000205 DAZBSA010000000
VERSION     DAZBSA010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.212"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..137)
                     /locus_tag="ACHGMT_002858"
     CDS             complement(<1..137)
                     /locus_tag="ACHGMT_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN6836540.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     I"
     gene            complement(134..787)
                     /locus_tag="ACHGMT_002859"
     CDS             complement(134..787)
                     /locus_tag="ACHGMT_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN6836541.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1075..>1687
                     /locus_tag="ACHGMT_002860"
     CDS             1075..>1687
                     /locus_tag="ACHGMT_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFN6836542.1"
                     /translation="MDNHTRKLLVLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
BASE COUNT          540 a          307 c          232 g          608 t
ORIGIN      
        1 tgaatctttt cttttctcgt ctccaaatat gttttaggaa agataaaaaa aattaatgaa
       61 tgaactaata taattattat tccaagaaca gaaaatattt gtatgaacat ttttggaaat
      121 atctttaatt ttttcatttt ctctccaatt tatatcctac atttcttata gtagttatac
      181 agtctaattg taattttttt ctaagttctt ttatatatac atctattact cgatcataag
      241 gagtttcttc gctatctttc catacataat caataatttg cattcttgtt aacacttgtc
      301 cattattatc caataaacat tttaatactt ctagttcttt tgcatttaca tctatttctt
      361 caccatttat tttagctgta tagctattaa aattaaccga tagatcctta tattcaaact
      421 tctctaaatg tccataattc ttcttaatta aagaatctat tctagctttt aagaccggca
      481 atgaaaatgg cttttctaca tatccgtcta ctaaattagt aaatgcatca attttatatt
      541 cttcatcact aaatgcagtc aagattagaa ttggtaaatt gcttttcttt ctaatttctt
      601 tcaacacttc taaaccattt ataaaaggta tctgaatgtc taagataacc aaatttatat
      661 cgctattaaa ttttgacaag gcttctcttc catctttagc ttgaataaca gtatatccaa
      721 attctgaaag atattcactt attccctctc ttatcatatt atcatcttca acagttaata
      781 ttttcatata tacctccact taagcctata cactagatat tatacagcat attatggctc
      841 tataatatct agtgttggtg acaccttctg ttgccaatac tttttctttt ttctaataaa
      901 tgcaaacaga caccttgaaa tcctttagaa taaagggttc tataatattt tgtgttgttg
      961 gaaaatcgaa ggcgattttc tactaacact ttttcttcgt ttcaactcaa aaagagactc
     1021 acttttggta aaatttagtc gaccaaaaca tcaccctacc aaaggagtcg ctctatggat
     1081 aatcatacta gaaaattact cgtattaaca gataaacatt tattttttga tgaagaatgg
     1141 ctgattgaaa aagaatataa aggcgttcaa gcgcagttta ttaaagggag attggatgac
     1201 tcaccttccc attgtgaaca ctgtggatcg attcgtatta tccgcaacgg ttcctatacg
     1261 acacgtactc agatattgaa agtcaaagaa aagttgacta ttttagaatt aaaacgtacc
     1321 cgttttctat gctatgattg tgggcagacc ttttctgcga aaacggactt agttgatgaa
     1381 catcatcaac taacaaagga actgaagcag gcgattctca tagaactgta cgaaaatcaa
     1441 tcacgtaaac taatcgctaa gaagtacttc gtctcggatg ggacggtgac acggatcttg
     1501 cgtgaagcaa cgaagcatta ccagccacgt atgaacttcc ttccgacggt gttgtgtatg
     1561 gatgagttca aatcaatgaa gtctgtttct ggttccatga gttttatttg tgttgatggg
     1621 acaacccatc agctatttac gatccttgaa gatcgcagac tttacaaact aactcaatac
     1681 ttcctgc
//
LOCUS       DAZBSA010000206         1671 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000206 DAZBSA010000000
VERSION     DAZBSA010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.213"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            18..1653
                     /locus_tag="ACHGMT_002861"
                     /pseudo
     CDS             18..1653
                     /locus_tag="ACHGMT_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          524 a          269 c          296 g          582 t
ORIGIN      
        1 gagtatataa ttaagtaatg caagtaaaga aaaaaataat attgtccatt ctcctagcaa
       61 ttattgctgg actttattca atcaattatt ttagaaatgt tcatactatt tctacatcgg
      121 gggatattgc cttccatttt gctagagtca aagggttatc ttcaattttt tctggtccaa
      181 tcaattttac gacgtttaat cattatggga gcggagtgaa ttatttttat ccctacttga
      241 ccttttttcc ggcagtcatt ttttattgga taagtaataa tttaatcgtt agttatatct
      301 tatatgtttg gctattgaat gtttgtacta tcatgctgat gtttcattat ggactaaaat
      361 ttttaaaaag aatcgatgcg gcttttatat ttagctgttt atatacattt tatgggtacc
      421 gtacgattga tatttatcat cgttctgcta ttgctgaagc gattgcactt acggttatac
      481 cgatagttat gtattatgct tacgcactga tttatgagaa aaagccatgt gcaattccat
      541 tagcagtcag tatgagctta ctcatttata cacatgttct ttccactttt atgagtgtaa
      601 tagccgttgg cttactagtc ttaggcggct ttgtagtaag tgaaacaaga aaaaaagaaa
      661 gcatccggtt gattgtggag ttgtcaaagg cagtaggaat gacgattctt ttaacagcat
      721 acttttggta tccgatgttc cgacaaatca tggttcaatc gattaatgag ccagataaaa
      781 cgaccttaca acaccaagca ttaaatgtct cggatagttt gattggtgcg atgaataatg
      841 acctgacaac ctttacggta ggaatcattg gtattatttc tttgatctgt ccactcttct
      901 tttttaaagg atttaatcgc aaagaaaaag tgatttacct tagtgcggta gtctcttggc
      961 tcttatccac caaatttttt ccatggtctt tattacaaaa tacacccatt caaatgattc
     1021 aatttccttg gcgaatctta ggctttcaag tactgtttgg ctcgttgatt ttatcgttcg
     1081 ttttcatgaa gagcacatca gctaagcaaa gaaaagtagt taatataacg gcaattgttt
     1141 tattactgtt ggctgtatct gctgcaacaa aagcaaacta tgaacataaa gttgtcactc
     1201 agagagatca cttggtaatg aatcaagaaa atcttattcg ttacacgaca ctccttcccg
     1261 gaggacttta tgactatgca cccacggaag cattacaata caaagaacat ttgcagaatc
     1321 atgaagtaag aattggcaag gaatggcata ctatgcctta tcaagtgatg gattcgaaaa
     1381 tagtctatac agtacatgaa tctaaaggac aaaaaataac gcttcctgta tttgattatt
     1441 ggggaacagt cgcaaaagta aatggacaaa aaagagagat tgataatagg actagtgtcg
     1501 attgaaggaa cacaaggaga aacaatcatt gaagtttctt ccatttatcc tccaactatg
     1561 aagttagctt ttttaattag tttatgtagt tacctctttc ttttaattag ttttcttatc
     1621 aggaagttac aacatataaa aataatgatc tgattacatt tttttcaata t
//
LOCUS       DAZBSA010000207         1666 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000207 DAZBSA010000000
VERSION     DAZBSA010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.214"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(106..483)
                     /locus_tag="ACHGMT_002862"
     CDS             complement(106..483)
                     /locus_tag="ACHGMT_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836543.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(783..1664)
                     /gene="galU"
                     /locus_tag="ACHGMT_002863"
     CDS             complement(783..1664)
                     /gene="galU"
                     /locus_tag="ACHGMT_002863"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFN6836544.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          462 a          370 c          244 g          590 t
ORIGIN      
        1 ttcttttata taagtgaaaa aagctttttt ccaccaatta aataaatctc tcatctcaat
       61 agtttatcca ttattttatc tttttttgcg tatacctact aaatattacc gtcttcttct
      121 cgttcttcta ctttgttcga gagaatctgt actagaaaaa gtagttgtat gtctaccttt
      181 tctgcctatc cgagactttc ttttcatata tgggatataa atgattagat acagtacagc
      241 aaaaaataaa gcaaatacgc aagcttccca actacctaac gccaattcaa ccatcaaggt
      301 agcaacccca acggatgctg cataccagat aaaaatcaga aacgtttctt tttttctgac
      361 catgcgaaac atgataaata gactaataag caatagtggc aacacaaata tccaattgct
      421 tccttccatc agccacttga gtatatcaag cagcgactgt gcaacagcga ctaattttcc
      481 catagtgaac acacaccttt ttttataatt aaaaaattcg cctttttatt ctactatgaa
      541 tcttctggct tggctttcat ttctcttatg taatcatttt cttttataca acttaaaaaa
      601 aaattaaacc attagcataa tattttctga atctattcac gaaagtcttt ttctatcttt
      661 cgttttccac gactcaactg atctagagtc aaaaaactaa aacgacagtt gcgcaaatga
      721 cgcacaagct gccgctttaa taacaaatag cctttcattc tcagcaggcc acttatttcc
      781 agttaacttt ctgttcttag ctttttactc aactggataa tatatgcact taatgaagtc
      841 ccaatttccg aatgttgaag tccatattca atatttgtct tcatatatcc taacttatcg
      901 cctacgtcgt atctttttcc tttaaactct ctcgcataga ccgcctctgt ctggttaaga
      961 gtctgaagtg catccgttag ctgtatctcg tttcctgctc ctggttcttg agtttctaat
     1021 atctcaaata ttttaggagt cagtagataa cgtccgataa tggctaaatt acttggtgct
     1081 tttcccattt caggtttttc tacaaaccct tttactctgc aacgaccctt ttcgctatct
     1141 gttttaggat caataattcc gtatttagat gtttcatttt ctggtacacg catgacggca
     1201 acagcggaag cttgtgtttc ttcataatcc atcatcaatt gttgagtcaa tgggatatca
     1261 tctttcatca aatcatcccc cagcatgacg acaaacggtt catttcctat aaatgctttt
     1321 gcttgaagca cggcatcccc cagtcctctt ggatgagatt gacggatgaa gtgaagattg
     1381 acttgagtcg tttcttcaac taaagcgagt aattctgttt tttccttttc ccgcaaattg
     1441 ttttccaatt cgatatttgc atcaaagtga tcttcaatgg gtcttttttc ttttcccgtt
     1501 acgatcaaga tgtcttcgat tcccgaatcc aatgcttctt ccacaataaa ctggatcgtt
     1561 ggtttatcca cgattggaag catttctttt gccatggctt ttgttgccgg caaaaaacgt
     1621 gttcctagcc cagcggcagg aatgactgct tttttgacct tcactt
//
LOCUS       DAZBSA010000208         1664 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000208 DAZBSA010000000
VERSION     DAZBSA010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.215"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACHGMT_002864"
     CDS             131..505
                     /locus_tag="ACHGMT_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFN6836545.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACHGMT_002865"
     CDS             complement(619..1248)
                     /locus_tag="ACHGMT_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFN6836546.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACHGMT_002866"
     tRNA            complement(1382..1454)
                     /locus_tag="ACHGMT_002866"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DAZBSA010000209         1653 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000209 DAZBSA010000000
VERSION     DAZBSA010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.216"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1604
                     /locus_tag="ACHGMT_002867"
     CDS             <1..1604
                     /locus_tag="ACHGMT_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFN6836547.1"
                     /translation="ETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHK
                     MFRPDNAYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPI
                     REGRTMEDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYN
                     GDINNRLARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFS
                     RTNRTYPEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQA
                     YGKLKEVSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQ
                     IQLLEEQIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTY
                     AANSSDAREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQAS
                     DNLIHAFSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFK
                     YPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          606 a          269 c          339 g          439 t
ORIGIN      
        1 tagaaacata tgcagagtat tcaccggaac agatcaatcg tatgatcgat caaatggagt
       61 ctgtcaaaaa agaaagttat ttggacccta ctgtttttga agcagaggaa catatccaaa
      121 aagtcattca taaaatgttc cgtccagaca atgcctatac aaaatttgat tttcgaaatg
      181 gccgtccaac gaagtctgcg atcttaacca cgagttccat tgatatggcc aaaaagtatt
      241 atcgggccat caaagagatg acgaaagaat ctgattggtt aataaaagag tttcccaatc
      301 agcctatccg tgaaggacga acgatggagg atcctgattt cccacgaatc gctattacct
      361 attcattaga agaaaatagc cgagattcaa gtgcccaaca agaagaaatg caaaaaatta
      421 ttgaggaata taatcagtat tatgatactg cctggtcact agcagacatt gagcgttata
      481 acggagacat caataatcgt ttggcacgaa aaagagcgga attcaaacaa tttgggaaac
      541 aaatcgattt agtaatcgta gtggatcgtc tattgacagg ttttgacgcc cctacgattc
      601 aaaccctatt tgtggatcgt aatctagagt atgcaggatt gatccaagcc ttttcacgta
      661 cgaaccgtac gtatcctgaa aaaacaaaag gactaattgt tactttccga aagccagcga
      721 caatggagaa gaatgtcgag gatgcgacaa aactgtattc agaagctaaa gaagaatcgg
      781 gtttgattta tccaagttac caagaatcca aaaaaagatt caagcaagca tatggtaagt
      841 taaaagaggt ttcatccatg gaatccattg atgaacatac gccacttgaa atccgtatcg
      901 agtacgtcaa agcatttcaa gaattgaatc gtgcgtatga atcgttagtg acttatgatg
      961 attacaatga tgacatggtg caatcttcaa ctttgaacaa tcaaattcaa cttttggaag
     1021 aacaaattgg cgtgtacgag accgtgaagg gttcattgat ggaggaagaa ccagaaaaag
     1081 agggagaaga tttttcaacg atcgaatttt atagtgaaaa taccactaag ttgtatgata
     1141 ttgattctac ttatattgat caattactag gaacttatgc tgcgaatagt tcagatgcac
     1201 gagaagaaat tgaaaaagct ttggctaaat tgaataaaac agaaggagtc aaagaagttt
     1261 accgacaaat tttaaatgca tttgatgtgg ggactttaga tagtaatgaa gatatctttg
     1321 tcattaaacg tcgctatttt acacaagcaa gtgataattt gattcatgcc ttttccaatg
     1381 agtggtttgt ttctgagaaa gaattacatg cttctaatct gcaatatatg ccaggtgaag
     1441 atccaattcc taatatgaaa gcaattatca acagtaagga ttacgaagga tataaagcca
     1501 aacatccaga agcgaaacca tttaaatacc cacaagaaat gaagcgagca tggcgtaaaa
     1561 tgttagatga tgagttgatt ccacttgaaa atgaattgag gtaaacgaga agagttaaga
     1621 aaattgtacg attcaaagat agtagtaaag tgg
//
LOCUS       DAZBSA010000210         1647 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000210 DAZBSA010000000
VERSION     DAZBSA010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.217"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..91)
                     /locus_tag="ACHGMT_002868"
     tRNA            complement(20..91)
                     /locus_tag="ACHGMT_002868"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="ACHGMT_002869"
     tRNA            complement(113..200)
                     /locus_tag="ACHGMT_002869"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="ACHGMT_002870"
     tRNA            complement(225..298)
                     /locus_tag="ACHGMT_002870"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="ACHGMT_002871"
     tRNA            complement(312..382)
                     /locus_tag="ACHGMT_002871"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="ACHGMT_002872"
     tRNA            complement(384..459)
                     /locus_tag="ACHGMT_002872"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
     gene            complement(478..551)
                     /locus_tag="ACHGMT_002873"
     tRNA            complement(478..551)
                     /locus_tag="ACHGMT_002873"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(515..517),aa:Met,seq:cat)
     gene            complement(564..653)
                     /locus_tag="ACHGMT_002874"
     tRNA            complement(564..653)
                     /locus_tag="ACHGMT_002874"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(615..617),aa:Ser,seq:tga)
     gene            complement(677..750)
                     /locus_tag="ACHGMT_002875"
     tRNA            complement(677..750)
                     /locus_tag="ACHGMT_002875"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(714..716),aa:Ile,seq:cat)
     gene            complement(764..837)
                     /locus_tag="ACHGMT_002876"
     tRNA            complement(764..837)
                     /locus_tag="ACHGMT_002876"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(801..803),aa:Met,seq:cat)
     gene            complement(865..937)
                     /locus_tag="ACHGMT_002877"
     tRNA            complement(865..937)
                     /locus_tag="ACHGMT_002877"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(902..904),aa:Ala,seq:tgc)
     gene            complement(957..1030)
                     /locus_tag="ACHGMT_002878"
     tRNA            complement(957..1030)
                     /locus_tag="ACHGMT_002878"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(994..996),aa:Pro,seq:tgg)
     gene            complement(1040..1113)
                     /locus_tag="ACHGMT_002879"
     tRNA            complement(1040..1113)
                     /locus_tag="ACHGMT_002879"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1077..1079),aa:Arg,seq:acg)
     gene            complement(1124..1209)
                     /locus_tag="ACHGMT_002880"
     tRNA            complement(1124..1209)
                     /locus_tag="ACHGMT_002880"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1173..1175),aa:Leu,seq:taa)
     gene            complement(1226..1297)
                     /locus_tag="ACHGMT_002881"
     tRNA            complement(1226..1297)
                     /locus_tag="ACHGMT_002881"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1263..1265),aa:Gly,seq:gcc)
     gene            complement(1309..1381)
                     /locus_tag="ACHGMT_002882"
     tRNA            complement(1309..1381)
                     /locus_tag="ACHGMT_002882"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1346..1348),aa:Thr,seq:tgt)
     gene            complement(1399..1480)
                     /locus_tag="ACHGMT_002883"
     tRNA            complement(1399..1480)
                     /locus_tag="ACHGMT_002883"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1444..1446),aa:Leu,seq:tag)
     gene            complement(1488..1560)
                     /locus_tag="ACHGMT_002884"
     tRNA            complement(1488..1560)
                     /locus_tag="ACHGMT_002884"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1525..1527),aa:Lys,seq:ttt)
     gene            complement(1564..1636)
                     /locus_tag="ACHGMT_002885"
     tRNA            complement(1564..1636)
                     /locus_tag="ACHGMT_002885"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1601..1603),aa:Val,seq:tac)
BASE COUNT          438 a          460 c          391 g          358 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca gtgtttttag tttttaattg
      481 cgggagcagg atttgaacct acgaccttcg ggttatgagc ccgacgagct accagactgc
      541 tccatcccgc gataatatga aaaaggagga taagggattc gaacccttgc acggttttac
      601 ccgcctgacg gttttcaaga ccgttccctt cagccggact tgggtaatcc tccatatgaa
      661 acttacaacg ccacaatgga ccttgtagga ctcgaaccta cgaccggacg gttatgagcc
      721 gtctgctcta accaactgag ctaaaggtcc aggctcttaa aaatcgcggc ggaggggatc
      781 gaacccccga cctcccgggt atgaaccgga cgctctagcc agctgagcta caccgcgaaa
      841 aagatattca ttatttaaat gaaatggagc ctagcgggat cgaaccgctg acctcctgcg
      901 tgcaaagcag gcgctctccc agctgagcta aggccccgtg tttaaattta taattatcgg
      961 gaagacagga ttcgaacctg cgaccccttg gtcccaaacc aagtgctcta ccaagctgag
     1021 ctacttcccg tttaattaac gcgcccaaga ggagtcgaac ccctaacctt ttgatccgta
     1081 gtcaaacact ctatccagtt gagctatggg cgcatatttg aaatgccgag gaccggaatc
     1141 gaaccggtac ggtgatcact caccgcagga ttttaagtcc tgtgcgtctg ccagttccgc
     1201 caccccggcg cgattgcttt ggcaaagcgg aaaacggggt tcgaacccgc gacccccacc
     1261 ttggcaaggt ggtgctctac cactgagcta tttccgcgtg tttaaagatg ccggctaaag
     1321 gacttgaacc ctcgaccctc tgattacaaa tcagatgctc taccaactga gctaagccgg
     1381 catgtaaaac ttaaattatg cgggtgaagg gacttgaacc cccacgccgt aaggcgctag
     1441 atcctaaatc tagtgcgtct gccaattccg ccacacccgc aaaaatatga gccgtacagg
     1501 gctcgaacct gtgaccctct gattaaaagt cagatgctct accaactgag ctaacggctc
     1561 atatggaggt taacgggatc gaaccgctga ccctctgctt gtaaggcaga tgctctccca
     1621 gctgagctaa acctccatgg ataactt
//
LOCUS       DAZBSA010000211         1642 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000211 DAZBSA010000000
VERSION     DAZBSA010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.218"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(164..400)
                     /locus_tag="ACHGMT_002886"
     CDS             complement(164..400)
                     /locus_tag="ACHGMT_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836548.1"
                     /translation="MKQNTSIAKNTTKFKCTAAAWRISISPTNRTNNRGMILFIWISF
                     FLLGYRVLPAYFAPPKRRGRLERLVRNKNHSLSY"
     gene            498..677
                     /locus_tag="ACHGMT_002887"
     CDS             498..677
                     /locus_tag="ACHGMT_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836549.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            808..1077
                     /locus_tag="ACHGMT_002888"
     CDS             808..1077
                     /locus_tag="ACHGMT_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFN6836550.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            1070..1327
                     /locus_tag="ACHGMT_002889"
     CDS             1070..1327
                     /locus_tag="ACHGMT_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFN6836551.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          602 a          227 c          319 g          494 t
ORIGIN      
        1 acgactttaa ctgatttttt agacacctat acttacgacg atacagaaga aatccaaaat
       61 gaacttgacc gtaaacgaat gccatatcaa cgatttgaaa ggaaaaaaag gcaataatca
      121 tttataccga aaagtgagaa tgtgtgaaat aaggacaaaa aacctagtaa ctaagggaat
      181 ggttcttatt tcgcaccaat cgctccaacc gcccccgccg cttaggtggt gcgaaataag
      241 cgggcaacac ccgataacct aagagaaaga aggagatcca tatgaataaa atcattcctc
      301 tattgttagt cctatttgtt ggcgaaatac ttatgcgcca tgctgcagct gtgcatttaa
      361 acttcgtagt atttttggca atactggtat tttgtttgat cttttggcgc tttgtattag
      421 cccttgtgag tctactattc aagatgattg cagccatatt tttcataggg ttatttcgat
      481 gaaaggagag agtactaatg ccaattgcaa taggaaataa acggttaccc gttacgttgg
      541 atgaaaagag acaaaaagaa ttgcagcaac taaagcagaa gtacggcaaa agtgaatcca
      601 ggattatgtg tattgcgtta gatttattga ttgcccaaga aaaagcagga tttgaggtac
      661 cagcactcaa aaagtgacgt cactttttat cctaaaaact aaaagtgata gcacttttaa
      721 ttataagaag ttagaatatt aatcatttgc ttaattgtac aatataatgt acaattgttt
      781 tatagaaata aataaggggt gaaaggaatg gaagcagtag cttattcaaa tttccgccaa
      841 aatttacgta gttatatgaa acaagttaat gaggatgctg aaacacttat tgtaacaagt
      901 aaagatgtag aagatacagt tgttgtatta tcaaaaagag attatgattc tatgcaagaa
      961 acgttgagaa cactttctaa taattacgtc atggaaaaaa ttcgtcgagg agatgaacaa
     1021 ttctccaaag gtgcatttaa aacacatgac ttaatcgagg ttgaatctga tgattaaggc
     1081 ttggtctgat gatgcttggg atgattatct ttattggcat gagcaaggaa acaaaagcaa
     1141 tataaaaaag attaacaagt taataaaaga tatcgatcgt tccccctttg ctggattagg
     1201 aaaacctgag ccattaaagc atgatttatc tggaaaatgg tccagaagaa ttacagatga
     1261 acatagactg atatatagag ttgaaaatga aacgatattt atttattctg caaaagatca
     1321 ctattaacca atcggaagta aggaaagggt cagaaactta aaagtttttg atccttattt
     1381 tatttaccct agtcatttaa aaagctaata tagcttagtg ttgattgtta ttaatgaatg
     1441 tgtttgttac gcgtattacg gatataaggt tagtaaaatc atttctaaag ttgaggaaaa
     1501 gtaaatataa atggcttaaa tttcaacaat ttgaagttga atagatatgt tataatacta
     1561 ttgtagtgtg ggatgttagt tactaaagga tgacgcttat atatatgact gaatagaata
     1621 agcaataggt ttaataatct at
//
LOCUS       DAZBSA010000212         1635 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000212 DAZBSA010000000
VERSION     DAZBSA010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.219"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1409
                     /locus_tag="ACHGMT_002890"
     CDS             <1..1409
                     /locus_tag="ACHGMT_002890"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFN6836552.1"
                     /translation="QTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLADQAK
                     QATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDV
                     EVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSV
                     FDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMNLRNG
                     DTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADFAFLL
                     HGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGTSIPT
                     TVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYAHLAN
                     FDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAISSLQT
                     TPENEAWLQGALEVFKHE"
     gene            1402..>1635
                     /locus_tag="ACHGMT_002891"
     CDS             1402..>1635
                     /locus_tag="ACHGMT_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFN6836553.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYN"
BASE COUNT          570 a          275 c          346 g          444 t
ORIGIN      
        1 aacaaacgat gttgtatcgc gaattacttt cagatgaaga atcaaaagaa gatctgattg
       61 caacaatcgt tgatattttg gggtatgcga tttcgccaga gtatcttttc aacgtcttag
      121 cggatcaagc aaaacaagca acgttccaat tgaatgatct aaacaaagcc tttgtgcaat
      181 tggcatctac ctacaatcaa tttaatgggt tatttgatga tgtggatctc caatcaaaaa
      241 aactaggaac agatgagcaa caacgaaatg tgacgattac cgaagtcatt aagaaattga
      301 atgacgtgga agttttaggt cacgatgggg acgtgatcgg ggatgcttat gaatttctga
      361 tcagtcaatt tgcttcagaa gcagggaaga aagccggaga attttacacg cctcatatgg
      421 tttctgatat gatggcacaa atcgtgacgt tggatcaaaa agaacgacga ttctttagtg
      481 tttttgatcc aaccatgggt tcaggttcat tgatgttaaa tgtgcgtaac tacttaactc
      541 acccagataa cgttaaatat catgggcaag aattaaatac aacgacatac aatctagcaa
      601 aaatgaactt gattttacat ggggtagatg ccgaagaaat gaatctccgc aatggggata
      661 cgttgaataa ggattggcca acagatgaac cttacacatt tgatgcagta gtaatgaacc
      721 caccttattc ggctaattgg tcagcagata cgaccttctt agacgattct cggtttaatc
      781 gttatggaaa attagcgcca aaatccaaag cagattttgc gtttcttctt catggtttct
      841 atcatttgaa ggaaacaggg actatggcca tcgtcttgcc acacggagtt ttattccgtg
      901 gcgctgcaga aggggtcatt cgtcaaaaat tactagaaga cggcagtatt tatgctgtga
      961 tcggtatgcc tgcgaacttg ttcttcggga catcgattcc aacgacggtg attgtattga
     1021 aaaagaaccg ccaaactcga gacgtattat tcattgacgc tagtcgagaa tttgtcaaag
     1081 gaaaaaatca gaataaatta tcggaagaaa acattcaaaa aatcctcgaa acctatgctg
     1141 aaagaaaaga tgtcgagaaa tacgctcatt tggcaaactt tgatgaaatc aaagaaaatg
     1201 actataactt aaatattcca cgctatgtag atacgtttga agaagaagaa ccaattgata
     1261 tggttcatgt cggaaatgac atcaagaaga tacgacaaga acagcaagtg ctagaaaaag
     1321 aacttttaga agctatctct tccctgcaaa caacacctga aaatgaagcg tggttacaag
     1381 gtgcgctgga ggtgttcaaa catgagtaat gatatacaac cagaaattcg tttcccagga
     1441 tttacggaag cttgggaaca gcgtaagttt gaatgcttac ttgataaaaa agatggagta
     1501 aggaggggac catttgggag tgccttaaag aaagaattct ttgtatctaa tagcaatttt
     1561 gttgtctatg aacaacaaaa tgctatttat gataattacg aaacaagata taaaattact
     1621 gaaaaaaaat ataat
//
LOCUS       DAZBSA010000213         1570 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000213 DAZBSA010000000
VERSION     DAZBSA010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.220"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            56..1522
                     /locus_tag="ACHGMT_002892"
     CDS             56..1522
                     /locus_tag="ACHGMT_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFN6836554.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          434 a          291 c          331 g          514 t
ORIGIN      
        1 tttcggtaaa gatgtataat attttctgaa aatttaaaga attggggtat agattatgga
       61 aaaacagcaa gacaagggct tcttcgggca gccgaaggga ttgtcaactt tgttcttcac
      121 tgagatgtgg gaacgattta gttattacgg tatgcgtgca ttacttatct attatatgta
      181 cgatacagtt gccaacgggg gacttggatt accacgagca acagcattag ctatcatgtc
      241 gatctacgga tctttgatct acatgtctag tatcgtcggc ggatgggtat ctgaccgagt
      301 gttaggagca aaacgcactg tattcttcgg tggagttttc attatgcttg gccacatcgt
      361 cttggcttta ccatttggtg taagtacatt gtttgtttcg atggcattga ttattatcgg
      421 aaccgccttt ttgaaaccaa atgtttctgg catggtcgga catctttatt caaagactga
      481 cttgcgaaga gatgcaggat tttcgatttt ttacatgggt gtcaacttag gggcattatt
      541 agcaccacta atcgttggta caatcggtca agaatataac tatcatttag ggttctcttt
      601 agctgctatt gggatgttct ttggattatt acaatacatc atccaaggac gcaagacatt
      661 agacggagtg ggtacaacac cgccgaatcc tttgacaact gaagaacgta aaggattcat
      721 ccgtaacttg atcatcgcac ttgttgttat cgctgcagta ttcggcgggg cacaactaac
      781 tggtcatctg acaattgact tttttgtcaa tacaatcagt gtattgggga ttatcttacc
      841 gttgtattac tttatcaaaa tgctgacttc aaaagatgta actgctgaag aaaaaccaag
      901 agtatgggct tatatcccat tattccttgc agcaatcatt ttctggtcat tacaagaaca
      961 aggttcatct atcttagctt tatttgctag tgaacgtaca caatctacgc tatttggtgt
     1021 aacgatccaa gcaagctggt atcaatcatt gaatccaatt ttcatcttta ttctgacacc
     1081 agtattcgta acgatgtgga cgaaacttgg tgaacgtcaa ccatctacag tagtgaaatt
     1141 ctctcttgga cttttatttg caggattatc gtacttgcta ttgatgttgc cgggtatgct
     1201 ttatggtaca agtggaaaag taagtccatt gtggttgttg ggaagtttct tcatcgtggt
     1261 tattgctgaa ttgtgtcttt caccagttgg tttgtctgtg acaacaaaat tagcaccacg
     1321 agcatttgaa tcacaaacaa tcgctatttg gttcttagct gatgcttctt cacaagcaat
     1381 caatgcacaa attgcacgct tctacacacc gggaacagaa tctgcttact tcggtattat
     1441 cggtgcagta gcaatcgtcg gtggtatctt gctatttact gtgaaagaac caatcaaaaa
     1501 actaatgggc tctattcatt aatcaatgaa cataaaaaca ggaattgctt atttgcagca
     1561 gttcctgttt
//
LOCUS       DAZBSA010000214         1488 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000214 DAZBSA010000000
VERSION     DAZBSA010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.221"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..198
                     /locus_tag="ACHGMT_002893"
     CDS             <1..198
                     /locus_tag="ACHGMT_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFN6836555.1"
                     /translation="IEDSLLINLYYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDAR
                     SDHRRYDYIKYTFVDPERYKD"
     gene            complement(234..605)
                     /locus_tag="ACHGMT_002894"
     CDS             complement(234..605)
                     /locus_tag="ACHGMT_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836556.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(607..1008)
                     /locus_tag="ACHGMT_002895"
     CDS             complement(607..1008)
                     /locus_tag="ACHGMT_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836557.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            complement(1022..1429)
                     /locus_tag="ACHGMT_002896"
     CDS             complement(1022..1429)
                     /locus_tag="ACHGMT_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836558.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
BASE COUNT          474 a          262 c          197 g          555 t
ORIGIN      
        1 atcgaagata gcttattaat caatctctac tattctaata agaactattt caaaattcct
       61 gctgcgaaaa ctagaatgaa gaagggtgta tactttttgt ttgatatcgt gactgacgtg
      121 ccggacgcac gtagcgatca tcggcgttat gactatataa agtatacttt cgttgatcca
      181 gaaagataca aagattaaat aaaaaaataa caaacaaact cgcttgaaat tcattaggtg
      241 agtttgttta ttattttttc tgcttctcga ttcatcagcg ccatatctgt tttcgtaatg
      301 gtttcattag gaatatagtc agcacatttt cttttctcta acattctata cccactatta
      361 tgtattccta aggtattcaa ttccttaact gttgaaaaca aattccaaac ggtttggtgg
      421 cttccttttc cgctacttcc attataaata atatcataat tttctttcaa atactcatca
      481 gattttttaa aagcactgta gtatgctctt gaaacagcag ttcttctata agattcttta
      541 gaattatatt tatcatctga ttcaatttgt ttggaaatag ataaatattc attaaagtca
      601 aacattttat tcccccaaag ttgctactac agatttcccc atatatttat agtcagataa
      661 tacattatcg agagtatcca aaacatcgta atctgaggaa aacaattcag atgttgtttt
      721 gataacaaag aaatacaagt ttaaatcttc atcatatttt atattgattt cagcttttct
      781 ttttaaaacc gtatttatca catcaaaaat tttgccatca ttacttgaaa ttgtgttaat
      841 ctcatttttt gtagtaatta caagttgatt atcattttta gttttcggag tagaacttgc
      901 atccacaaca actggagatg ctagaattac cgatgcgcaa gcaaaaatcg cagcaaaatt
      961 tattaagcta ccactccgat gtgtatttga tggtactata tcaagcatcc gtatcgtctc
     1021 cttaattttt ttccactggt ttgttgacct tgatatcaaa gatttgcctt ccaatctcag
     1081 ttccattaaa tttaacaaca gcataatgct ctcctgcttc ttcaaccaag acatttctgg
     1141 cgtttatatt aaagtttata tcgttaactt gaggaggcaa gcttaccgat acttcacccg
     1201 tagaaaaaat ttgtttttca ttcttgttta aaacttcaat ttctactatt tttatctttt
     1261 ctgtatcctc tatctcagac accacagctg ttacagaaaa tgataaagca gtaggaatta
     1321 aaggcgcatt aatagaaaga gctggattaa ttactttatt aataacatta gtgccaaatt
     1381 gctgactttc tacatcttct gaaattatta attgtactct ataagacatt gatttctcct
     1441 aatttttaca tttatttaat aaatttatgt ttcacttttg ctagtcaa
//
LOCUS       DAZBSA010000215         1483 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000215 DAZBSA010000000
VERSION     DAZBSA010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.222"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..972
                     /locus_tag="ACHGMT_002897"
     CDS             64..972
                     /locus_tag="ACHGMT_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFN6836559.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..>1483
                     /locus_tag="ACHGMT_002898"
     CDS             972..>1483
                     /locus_tag="ACHGMT_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836560.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGV"
BASE COUNT          511 a          216 c          345 g          411 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taagaataca gaggttatgg aagcgactat agttgaaaat
      181 ggtcttccct acgatttatc agaatgtaag gtatattttg aatcagttgt aggtgggaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat taactatatt
      301 tttgataaat attccatgca gtgtttacat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcattaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaatcg aagatttaat tgcggatatg
      481 acggacttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgaac gttggagaca agaacaggaa cacagtttcc aagattggag agaaggacaa
      601 gaatcagact atttggcatg gtttgaatca atcaaggata ttttaaagac gattgatcca
      661 ggtggagtca tgttagccga actaacggat gcgcgagtag atattcaagg agttcgtcat
      721 gcgtctattt cagagcgttt gctggcagat atggactatt tatatcagaa attgcgagca
      781 acgcttttca cgattgaata cggggagatt gaagtgactg atattttgca ggatgatctc
      841 ttttcagata atcatgaagt cgaaaaagtt ggaactgtag cattcccgat tgaagaagga
      901 gccttgatca tcgcaaccgt tgatgatcca aagcaaaatg tgttcactct tgagaaagtt
      961 ggggtgatct aatggctaaa actaaacgaa tgatggaaac ggatgaaaaa acgggtgtac
     1021 aacgccaatt ctttccaatc acacatgctt ccgcagttct tgggttagaa gaaataatag
     1081 caggagaagc aacagtttta tctgttaatg gaaaaattgg agccgtcgtc attactaaag
     1141 aagatttggg attagagaat gttctcacag aattacccta tgcaagtgaa gaagatgacg
     1201 gcattatcac ggcagaaatg tatcaaaaaa ttttaaacag tggagaaggt gactacgtgt
     1261 tgccagttgc cactatcgac cgtttgggtg gcataaaagt tggtgaatta ttgacgattg
     1321 atgagacagg gaaagtctct gcagtcagac aatctgatgt caatttttcg ctggagttaa
     1381 aagaaaaact cgattcactg aaaaattata ctgctggaga aaacatcact attgatgaag
     1441 atggaacaat tagttctaca ggtggtagtg ggactggtgg tgt
//
LOCUS       DAZBSA010000216         1480 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000216 DAZBSA010000000
VERSION     DAZBSA010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.223"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..972
                     /locus_tag="ACHGMT_002899"
     CDS             64..972
                     /locus_tag="ACHGMT_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFN6836561.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..>1480
                     /locus_tag="ACHGMT_002900"
     CDS             972..>1480
                     /locus_tag="ACHGMT_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836562.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGV"
BASE COUNT          529 a          197 c          316 g          438 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taaaaacact gaagttatgg aagctacaat tgtcgaaaat
      181 ggtcttccat atgatttatc agaatgtaag gtatattttg aatcagttgt tggcggaaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat caaatatatt
      301 tttgataaat attccatgca gtgtttgcat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcatcaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaattg aagatttaat tgctgatatg
      481 acagatttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgatc gctggagaga ggaacaggag aatagtttcc aagattggag agaaggacaa
      601 gaatcagatt acttgtcatg gtttgaatca atcaaggata ttttgaagtc tatcgatcca
      661 ggtggagtaa tgttagccga attaatggat gcacgtgtag atcttcaagg tgtacgccat
      721 gaatcgattt ctgaacgttt cctatccgat ctaaattatt tgtatcaaaa aatgaaagca
      781 gcacttttca cgattgaata cggtgaaatt gaagtgactg atattcttca agacgatctc
      841 ttttcagata atcacgaagt cgaaaaaatt gaaacagtag aatttccaat tgaagaagga
      901 gctttgatta ttgcaactgt tgatgatcca aaacagaatg ttttcactct tgagaaagtt
      961 ggggtgattt gatggttaaa gtaaagagaa tgatggaaac tgatgaatct ggtgttaaac
     1021 gtcaatttat gccaattaca catgtctctg cagttttagg cttagaaaaa atgatatcag
     1081 gacaatcaaa agttctttcg gttaatggta aatatggtgc agtcatttta acgaaagctg
     1141 atttaggatt agagaatgca attactgaac taccttatgc aagcgagaca agcgatggta
     1201 ttcttactgc tgaaatgttt caaaagatcg taaatggaga aggtggaaca tatattttgc
     1261 cgattgctac tcctgaacaa ttaggaggta taaaagttgg ggaactctta gaagttactg
     1321 aagaaggtgt tttatctgca actaagcaaa cagacttcaa tttctctgaa gaattaaaag
     1381 gaaaattaga atctctaaaa atattaaaag caggtgcaaa tatatcaatt gcagaagatg
     1441 gaacaattag ttctacaggt ggtagtggga ctggtggtgt
//
LOCUS       DAZBSA010000217         1458 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000217 DAZBSA010000000
VERSION     DAZBSA010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.224"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(428..1042)
                     /locus_tag="ACHGMT_002901"
     CDS             complement(428..1042)
                     /locus_tag="ACHGMT_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFN6836563.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          476 a          278 c          242 g          462 t
ORIGIN      
        1 tatttctatt agaaaaaggt atcctgttgg acaattttgt tatatcaggt aaacttcatt
       61 gcaataatga taacaaatgt actataatta ctttgtaaag agttttgggg aagactcttt
      121 catcaacgtg tattaagaga caagaaagaa tccatatgga ttctttcttg tctctttttc
      181 cactactttt tatttttctg acactacata ttcttataac tcatttctca taactgtgaa
      241 acaacttata aaaataacaa actttaaaca aaacaagctt aaaatcaaag atgatccatc
      301 ttaaaataac taaaacaaaa aaagtagtcg ttgttaatcc tcattaataa tcgttttctt
      361 tttttcaaac gctccatttt tatgtggaac attatttttt taaggaactt tgtcaacatg
      421 tggataacta ttttccactt gtgtcctgga ctagctgttt tgctgtaaaa tacaaaactt
      481 gctgcagcaa aaagatcgtt gcaagagagg ctgttccgca gatcgctgga tctagatttg
      541 gcaaagtcaa gactgcatca tgtaaattgg cgtggttgtc caaaaccaaa tcagcaaatt
      601 caaataatcg caaaccactg ctatgttgcg atttgatact tcgtgacaaa tcaaatccag
      661 taacaatgat taatggatga ccattttctt tgatccactg agctagttcg atgattgccg
      721 ggtttcttcc ttcattcgaa agaagaagaa aaatgtcttc tgatttgaca gaaagctgat
      781 caataattgc ctgtcctgca ccttctagcg tttcatagac acctccggct ggattttcaa
      841 tcaatatact tggtacaaga tccggggttt gatcccaaaa ttcaaatgct acaccactta
      901 gtgtgcggct agcatatatc tgcaatctac caccttgatt aatttgtttc gcgatccaag
      961 tgccagcttg aagcatcagc ggctcttcta tccttaattt ctcttccaat tggtgataat
     1021 agtcaaaata acataaactc atttatctct cccgcctagc tatacttgac atgaatagaa
     1081 acagctgcct aatctaagga agtacataaa aaaacagtag ttagacagta acacatgtgg
     1141 tctttgctgt ttcaaacaca aaaaaggcat tgaacgaata cgcccaatac cttgacatag
     1201 caaataaaac aacgtgcaga ccatggaaaa tatctacata ggggtagaat tttcacccta
     1261 gattaggtac aataaattag gtcgatgcac tgcattgact atggcaaaat gatcggtcag
     1321 ggtgtatgtg tgtgactggt cattccctga ttcttagcgc caactaaggg tcagggagcc
     1381 tttttttatt cagctgtttc tatctgaatt tattgtaagt aatctcttat ccaagtgcaa
     1441 gcaagaaaat acaaaaaa
//
LOCUS       DAZBSA010000218         1446 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000218 DAZBSA010000000
VERSION     DAZBSA010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.225"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            76..1305
                     /gene="arcA"
                     /locus_tag="ACHGMT_002902"
     CDS             76..1305
                     /gene="arcA"
                     /locus_tag="ACHGMT_002902"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFN6836564.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT          501 a          253 c          306 g          386 t
ORIGIN      
        1 gagacaaaat tatgaaagtg attacaaaag gaggatatcc tatacatgta atcaaaaaga
       61 aggaggcaac acaacatgga taaacctatt cacgttttct ctgaaatagg gaaattaaag
      121 acagttttac tgaaaaggcc tggacaagaa gtggaaaatt tgacaccaga tattatggat
      181 cgtctgttgt tcgatgatat tccatatctt ccgattgctc aagaagaaca tgacaacttt
      241 gcaaagacat tgcaaaatga aggcgtcgag actctttact tggaaaaatt agcggcagaa
      301 gcgattgacg caggcaatgt caaagaacaa ttcttgaata aaatgttgga tgaatctcat
      361 attgcatcga atgctgtaag agagggtctt catgagttcc ttctatcaat ggaaacgcaa
      421 gaaatggtag ataaaatcat ggctggggtt cgtacaaaag atattgaagt acgttctagc
      481 agtctatacg atctgtcagc agacgatgac tatccatttt acatggaccc aatgccaaac
      541 ttgtacttta cacgcgaccc ttccgcatca atgggaaatg ggatgacagt caataagatg
      601 acttttgaag cacgtcgtcg tgaatcgatg tttacagaat atattttgaa acatcatcca
      661 agatttgcaa ataaaggaat cgaagtatgg ttggatcgtg aaaatccaga tcatatcgaa
      721 ggcggagacg aattgatctt aagcgataaa gtagtagctg ttgggatttc tcaacgaact
      781 aatgcgaaag ctttagaaaa attagcacgc cacctattcg caaaaaattc aggatttgaa
      841 aaagtgttag caatcaaaat tccaaacaac cgtgcaatga tgcatttgga taccgtattt
      901 acaatggtag atcacgataa attcactatc catccagcaa ttcaaagtaa agacggtaag
      961 atggatgtct ttacaatcga accagatggt gatgatatca agattactca ttcagataac
     1021 ttacacgaaa cgatgaaagc tgctcttgga ttagatgatc ttgtactgat cccaactggt
     1081 aatggagatg agatcgttgc acctcgagaa caatggaatg atggatcaaa cacattggca
     1141 atcgctccag gtgttgtagt cacttataac cgaaattatg tatcgaacga attgcttcgt
     1201 agctatggca tcaaagtgtt agaaatcaat tcaagtgaat tgtcacgagg ccgtggtggt
     1261 ccacgctgca tgagccaacc acttgtccgt gaagacttga agtaatttgt ttcctgacgg
     1321 cggtcttact gtcagctgcc gtcgggattt cctctttaat cttaaataaa aacaatctaa
     1381 aggagaatgt ttaatatgaa agaatcagta ttccaaggaa gaagcttatt agcagaaaaa
     1441 gacttt
//
LOCUS       DAZBSA010000219         1434 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000219 DAZBSA010000000
VERSION     DAZBSA010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.226"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(778..1119)
                     /locus_tag="ACHGMT_002903"
     CDS             complement(778..1119)
                     /locus_tag="ACHGMT_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFN6836565.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1119..1349)
                     /locus_tag="ACHGMT_002904"
     CDS             complement(1119..1349)
                     /locus_tag="ACHGMT_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFN6836566.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
BASE COUNT          452 a          239 c          206 g          537 t
ORIGIN      
        1 aaaataaaaa aaaccttaca ttatactgga atgaactgta ttgatagtgt acctaaaagc
       61 tctactgaca taagatcgtg taatcggtaa agtaatttta ttaattatac gggatgtaat
      121 cgattagaga ataaactaat cataaaaggt acttttacat tcataattgt cgctttgaga
      181 ttctactttg gttcactatc tgacccaatt gcacattttc gatcattaca agtattggtg
      241 tcgttagaac aactaaataa aaactagcgg tagatttttc agaatctatg ctaatttctg
      301 tatggatgtt ccagtgatgg caaatttctc ttttcattgt ttttcttgtc gcatgatatg
      361 cctttttgtg ttattcagtt aaactataga aattctttcc tagacagatc ttgatcccaa
      421 attttacgtt tttgagcatc gatatagttt aaaatgacat tactcatatc taaagagttt
      481 agatttttta ggactaaaac tgtcagttgt tctttttctt ctttctgtta aagtattgtc
      541 gcgacttttc gtatacgtta agatatgtcg tgaaaaaaca agttttgtga cacaaaaaaa
      601 cgaatgactt gaggaaatca cctcaatcat tcgtttaaaa tgctatatat tctttagttt
      661 aagatacgga aattaatgcc atcgaccaca ctagtagaat gaacgacatt tacttggcct
      721 ttttttaatt tcgttatgtt tagcatataa aaatattata gagtttactt acaatgctta
      781 aaacatatac tgtataatct gatcaatttt agccatgtct tgcaatggta agttttctat
      841 ttttttgagt tttcgttcct taaaatcaag agattttaat tgtgtaataa ttacttgtcc
      901 ttttatatct agctcttcag gtaatgagta acgtgtcggt aaattcttta tagtggaagt
      961 aattggacat atgaccgaaa acattgtttt acggttaaat tcataacgag agacgactag
     1021 tcctggtcgt cttttttgaa tttcttttcc tacggaggga tcaaaatcaa tccatacaat
     1081 atcacctttt ttaggaatat aaattctttc ttctgacact ataaaatttc tctcccttca
     1141 ggagttaaat cttcccattc gtctttctca taatactcgg cctcttcccc accactaaat
     1201 ggatcttcaa tttttggaac taacgtaatt gttccatcat cagaatacac aacgatatat
     1261 tcttggtttt ctgaaggttt ctttccgtta tcagaaggta aagttaccac tacagaactt
     1321 ccttgtaatc tagattttgt aacaagcatt atcatcactc ccttaataaa agtatactat
     1381 ataacactaa gtaacgcaaa attccccttt gtaaactatc tagaaaattg tttt
//
LOCUS       DAZBSA010000220         1417 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000220 DAZBSA010000000
VERSION     DAZBSA010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.227"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..976
                     /gene="bsh"
                     /locus_tag="ACHGMT_002905"
     CDS             2..976
                     /gene="bsh"
                     /locus_tag="ACHGMT_002905"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFN6836567.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          493 a          183 c          236 g          505 t
ORIGIN      
        1 tatgtgtacg tctattactt atgtaacaag tgatcattat tttggaagga attttgatta
       61 tgaaatatct tacaatgaag tagtcactat tactccaaga aattataagt tgaattttcg
      121 aaaggtaaat gatttggata ctcattatgc aatgattggt attgccgctg gtatagctga
      181 ctaccctctt tattacgatg cgacaaatga aaaaggattg agtatggctg gactaaattt
      241 ttctgggtat gctgattata aagaaataca agaagggaaa gacaatgtat ctccttttga
      301 atttattcct tggattttag gacaatgctc aacagtagga gaagctaaaa aattgttaaa
      361 aaatatcaat ttagcaaata taaattatag tgacgaactt cctttatccc ctttacattg
      421 gctattagct gataaagaaa aatcaattgt cattgaaagt atgaaagatg gacttcatat
      481 atatgataac cctgtgggcg ttcttaccaa taatccttca tttgactatc aattatttaa
      541 tttaaacaat tatcgtgtct tatcgagtga aactcctaaa aataattttt caaatcaaat
      601 aagtttgaat gcctatagcc gcggtatggg agggataggc ttgcctggag atttatcctc
      661 agtatctcgt tttgttaaag cgacttttac gaagctgaat tctgtatctg gagattcaga
      721 gtcagaaagt attagtcaat ttttccatat cttaggttca gtagaacaac aaaaaggttt
      781 gtgtgatgtt ggtgatggaa aatatgaata tacaatttat tcttcttgtt gcaatgttga
      841 caaaggaatc tattattatc gaacatatga agacagtcaa attactgcaa ttgatatgaa
      901 taaagaagac ttagatagtc ataagttaat tagttatcca attatagaaa aacaacaaat
      961 taaatatata aattagttaa tgtgttgtga ttgattattt aatatagata taatacaaaa
     1021 aggcaaagat attttcaaaa cattttttct tgatgtgata tccttgtctt ttttgtaatg
     1081 tttttaactt attgcttttg caaaatttat ttacctgttt tctggatcaa cgatagtgta
     1141 tattatgcta tccttgtttg gattttagga cgtttaaaga ggcacttttt tacataactt
     1201 aatccataga ccttttaagc atgctgtgag ataataataa agaagagttt aaagcgcacc
     1261 ccaaaccact tccccataag tgtattacgc tttaaactct tttatatttg aattcactaa
     1321 gaagcatacc atatttttga aaaaaagtga gaaaaaaggc ttataattag gatttagagt
     1381 aattaattag atgtttattt tataggactg atactat
//
LOCUS       DAZBSA010000221         1364 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000221 DAZBSA010000000
VERSION     DAZBSA010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.228"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACHGMT_002906"
     CDS             complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACHGMT_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="HFN6836568.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          393 a          242 c          216 g          513 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgcc
//
LOCUS       DAZBSA010000222         1363 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000222 DAZBSA010000000
VERSION     DAZBSA010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.229"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            103..1335
                     /locus_tag="ACHGMT_002907"
     CDS             103..1335
                     /locus_tag="ACHGMT_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFN6836569.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
BASE COUNT          444 a          258 c          301 g          360 t
ORIGIN      
        1 aaataacctc ctttttttat ttaaagcctc attgtttgaa tcataattaa atggtatcat
       61 gctaatagtt accagaaaaa atggtgagga ggaattttta ttatgttatc tgattttaat
      121 gaacttctaa aaaaatatgc acgcttaatt gcagaaactg gcgtagccac agaaaaagga
      181 cacacagttg ttttacagat cagcgtggaa caggcacccc ttgctcgatt gatcacacaa
      241 gaagcttata aactaggagc cgctgaggtt atcgttcaat ggactgacga ccagatccaa
      301 agagaatttc ttttacatgc tgcaacggat cgtattgaga atgtaccaca atctaaaatc
      361 gatcaagcgg atgaatggct tgaaaaagga gcaagcagga tcagcgttgt ttctgccgat
      421 cccgatgcat ttgctggtgt agatagccat cgagtagcgt cttatcaatc agcgaatggc
      481 aaagcactaa tgaatttacg caaggcaact caagcaaata aagtcagttg gactgtcgtg
      541 gctgcggctg gtaaacaatg ggcagcaaaa gtcttcccag aactgccaga agaaaaacaa
      601 gttgaggcat tatgggatca gattttcaaa acgactcgtg tctatgaaga agaccctgtc
      661 ttagcatgga agaagcatga tgaaaaatta gcaaaaaaag cagaagaatt gaatcgagaa
      721 caattttctg cccttcacta tactgctccc ggtacagata tcattatcgg attgcctaaa
      781 aaccatcttt gggaaggcgc tggaagctat aatgctcgtg gagaaaaatt catggcaaat
      841 atgccgacgg aagaagtgtt cacagcacct gacagtcatc gagtagatgg ttacatttca
      901 agtacaaagc cactgagtta tgctggcacc atcatctcgg gtatgaaatt tacttttaaa
      961 gatggaaaag ttgttgattt ttctgccgaa caaggagaag atgtcttagc aaaactgctt
     1021 gatacagatg aaggtgcgcg tcgtttagga gaagtcgcac ttgtacctga cccttcacct
     1081 atttctcaat ctggcatcat ctttttcaat acattatttg atgaaaacgc atcgaatcac
     1141 ttagcactag gttctgctta tgccttcagt gtgaaaggcg gtacagaaat gtccgatgaa
     1201 gaattagcag aagctggatt gaatagaagt caaacccacg tagatttcat ggtaggatct
     1261 gacaagatgg atatcgacgg aatccgtgaa gatggctcta cagtaccgat tttccgaaat
     1321 ggcgattggg cttgagtata ctttcgcaca catgcaaaaa ata
//
LOCUS       DAZBSA010000223         1362 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000223 DAZBSA010000000
VERSION     DAZBSA010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.230"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1362
                     /locus_tag="ACHGMT_002908"
     CDS             82..>1362
                     /locus_tag="ACHGMT_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFN6836570.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          464 a          262 c          257 g          379 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat ta
//
LOCUS       DAZBSA010000224         1355 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000224 DAZBSA010000000
VERSION     DAZBSA010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.231"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1355
                     /locus_tag="ACHGMT_002909"
     rRNA            <1..1355
                     /locus_tag="ACHGMT_002909"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DAZBSA010000225         1338 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000225 DAZBSA010000000
VERSION     DAZBSA010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.232"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(4..636)
                     /locus_tag="ACHGMT_002910"
     CDS             complement(4..636)
                     /locus_tag="ACHGMT_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFN6836571.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            987..1286
                     /locus_tag="ACHGMT_002911"
     CDS             987..1286
                     /locus_tag="ACHGMT_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFN6836572.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          486 a          192 c          215 g          445 t
ORIGIN      
        1 tgattatact acttttattt gtaaaaattt tctagcaaaa ccaagtgctt gcaactgatt
       61 aagcgtacaa ttttttagat gttccgagcg tacttcaatc atttcaaagg agttgctact
      121 taaatcgata ttttttaaat cagtatcaaa aaagctagca ttttctaaat cattagtaga
      181 aaacattatt ttagtgaggg aacagtctac aaaatctgcg cttgataaga cagagtgctc
      241 aaaggtactt tcttttatgt ttgaaccaga taaatttaca tagtccattt tacaattctc
      301 gaataacgtg ttcccaatat aactttccat caatttagta cctgttaatt tacaattgac
      361 aaatttcacg cgatgcaaca tgctttttgt caatatacag cctgaaaaat cacagttttc
      421 gaatatacag tcggtaatct ccattctttg aaaatcattg gaagcaaaaa atgcatactt
      481 gaaaatagat ttgtaaataa ttggatggca gattatttgg ttttcgatga cctctttttg
      541 tgaaaggata ttatacagtt caaaatcttc gttttcatag atatgaaaaa aggatccttt
      601 attcaagctt gcttcattaa ctttaggaaa aatcatattt aattacctcc attttcataa
      661 agtcatatta acaaaagcta aaccaactta cattagcaga aaatgagata ggttttcgcc
      721 tttttcgata acgatagtgt cgctatctct tgaaacgttc ttagaattgc cctgagagac
      781 acaaattagg cgtaattcta ataaaaatct tagaataaaa atttttactg atttaattta
      841 gaatgttttt aaaataaaaa tcgttgccat aacagtgtat tgaaaggttt taacaatcca
      901 gtaatgattt aaaaaaatct agatgtagcg tatactttgt ttatagaatg tagtgggaag
      961 aaatatattt ttgggaggaa atcgtgatgt ctaaaagtga agaaatagaa aacatggtac
     1021 taaaattata tgatctcgtt ttaaatccag agatcaaaga aaaggaacgt atacttttga
     1081 tagacgcaaa gacaggctta gaaaaaggtc agtattatcc taaagtcata aataatttgg
     1141 aacgtagttt acgtccgtta gcaatcaggg gagaattgtc taaaccagta tcaccgtttt
     1201 atatggagat atcaacaata ggaaaatttg aaaaagaatt aggtcgagga atggcttcgg
     1261 caccgattac ttttgggcat ttataaaaac gagttattca tctcaatgaa ttaaaaagta
     1321 tagtcaatct cataatgc
//
LOCUS       DAZBSA010000226         1324 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000226 DAZBSA010000000
VERSION     DAZBSA010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.233"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..820
                     /locus_tag="ACHGMT_002912"
     CDS             278..820
                     /locus_tag="ACHGMT_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836573.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            976..>1324
                     /locus_tag="ACHGMT_002913"
     CDS             976..>1324
                     /locus_tag="ACHGMT_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFN6836574.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          533 a          157 c          248 g          386 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc tggatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccag
//
LOCUS       DAZBSA010000227         1314 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000227 DAZBSA010000000
VERSION     DAZBSA010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.234"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..708
                     /locus_tag="ACHGMT_002914"
     CDS             <1..708
                     /locus_tag="ACHGMT_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFN6836575.1"
                     /translation="KQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGI
                     VNSAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKD
                     TIKGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVK
                     TPSVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELT
                     RQMKRDAGAVGGTLYAT"
     gene            698..>1314
                     /locus_tag="ACHGMT_002915"
     CDS             698..>1314
                     /locus_tag="ACHGMT_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFN6836576.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNL"
BASE COUNT          504 a          155 c          243 g          412 t
ORIGIN      
        1 aaacagtttt tcaaagatac ttggaaaaat attaaagata cggcatacaa tacgtggatt
       61 tctataaaaa ataccatgat taatacatgg aataacatta aggactcttt ctggaatatt
      121 gttactggaa ttgttaattc tgctgaaaat gcatggacaa atctaaaaaa tggtgtttca
      181 aatgctatta atcgggtaaa agaaatcttt aattcgttaa gggaagtcaa tttatttgaa
      241 attggcaaga acatcattga tggacttata aatggtgtaa aagaaaagtg gaatgctttg
      301 aaagatacta tcaaaggtat tgcaggtaat atcaaaaatt ggattaaagg agctttagat
      361 attcattctc catcaagatg gatgcgtgat atggttggta aaaatattgt tcaaggtatt
      421 atcgttggta ttgataaaga gcaaagtaag ttagatcaaa ccatgacaaa tttagtaaaa
      481 acaccgtctg ttcaacctgt agtaaatgga ataagtcacc taccaattac acaaacaaag
      541 caaaatatat ctgctagtga aacaaaagaa attcatttac atttaaatgt ttatggagat
      601 ttacctgatt caatggttcg aaagattact acaaaaatta aaacagaatt gacaagacaa
      661 atgaaacgag atgctggtgc agtaggagga acattatatg caacttaaga gaggacaatt
      721 ttttattaat caacattatt cttctgaatt taatgtgtat attcaaaaca ggcctgcttc
      781 tgtttcagct agtcgagtaa ttgaattgag agagcgagaa ggaaatgatt ctatcattat
      841 tgataaaggc tattacaaaa atgtgactag aaagattgag tgttactata aagcaccttc
      901 aattgatatg gtgcaggagt gggaagatcg aatcactgaa tggttagata tgtcgtctta
      961 tagtgatttt attctttact atgatgaaca atatatttat caagctgtag taatagaagg
     1021 acctgaattt aaaggaacaa gaaaaacagg aaatattgtc ccttttgaat ttacagtgag
     1081 tattcgacca tttaaggaaa attacaatgg tagatttact attcgacaaa cagaaacttt
     1141 tgaaatttat aacccagaga agtattcttc aaaaccgctt attaaattga gtggttctgg
     1201 agatgcttct ttttatatta ataaggataa atatgatttg aaatcgttgg acagagaatt
     1261 atttatagat tctaaattag aagaggctta tcgaaaatta gatggtaatt taga
//
LOCUS       DAZBSA010000228         1251 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000228 DAZBSA010000000
VERSION     DAZBSA010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.235"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..1008
                     /gene="scrK"
                     /locus_tag="ACHGMT_002916"
     CDS             136..1008
                     /gene="scrK"
                     /locus_tag="ACHGMT_002916"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFN6836577.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          385 a          225 c          288 g          353 t
ORIGIN      
        1 taaaaaatga taaaaattca aaaagaaaaa atagtagtta tttacttgtc atgttactaa
       61 ataaaaaaat aatttaaata tgatatatag tcatttattt agtgatttca ttctaaaata
      121 aagggagaaa tgtctatgct atacggtgca attgaagcag gcggcacaaa atttgtgtgt
      181 gccattggcg atgaagaaat gacgatcaag gaaagagtga gctttccgac aacgacacct
      241 gaggaaacaa tgcctttagt cattgacttc ttcaaacaat accaagcaga tttagcaggg
      301 attgggattg gctcatttgg gcctatcgat atccatcgcg attccgccac ttatgggtac
      361 attacctcga ccccgaaatt ggcatggcaa aattttgatt tcatcggtac catgaaaaaa
      421 gaatttccga tcccgatttc ttggacaaca gatgtcaatg cggctgccta tggtgagtat
      481 gtcttcggca gtggcaaagg attgagcagt gtcgtttact atacgatcgg tacgggtgtt
      541 ggcggcggtg ccttacaaga agggcgcttt atcgaagggt ttagtcatcc agaaatgggg
      601 catatgctcg tcgtccctca tccaaaagat gactttgctg gcagctgtcc atttcatggc
      661 aactgtttag aagggatggc cgctggaccc gccatcgaaa accgcttagg gaaaaaagga
      721 caagaggttg cagaagatga tccttattgg gaaatcgaag cctcctatat tgctcaatgt
      781 gcctacaata caaccttgat gttttctcca gatgtgatca tttttggcgg tggtgtgatg
      841 aaacaacgcc atatgctgca aaacgtccac gacgcatttg caaaattggt caatgggtat
      901 gtcgaaacac ctgagttaag caaatatatc gtgaccccag cattagaaga caacgctgga
      961 acgctcggtt gtttggcatt ggctcgagaa gcggtcttac attcataaac gaaaaaaggc
     1021 tcttcgacat ttagtgttgg tagagaaaga tacaactgaa aatttagtca taaccagtta
     1081 ggcagagtgc ttaactggtt tttgttttac ttggaagatc aagccttgaa tgagataaat
     1141 tcttgctcga aagttatgga aatttcgata gccgtaggcc acttgtattg tgcaatagtt
     1201 ttttacaaaa aaaagggggt gcggatattt ttttggacct ttttaacaaa t
//
LOCUS       DAZBSA010000229         1234 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000229 DAZBSA010000000
VERSION     DAZBSA010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.236"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..1015
                     /locus_tag="ACHGMT_002917"
     CDS             8..1015
                     /locus_tag="ACHGMT_002917"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFN6836578.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1027..>1234
                     /locus_tag="ACHGMT_002918"
     CDS             1027..>1234
                     /locus_tag="ACHGMT_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HFN6836579.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQF"
BASE COUNT          485 a          160 c          235 g          354 t
ORIGIN      
        1 gatataagtg tttaaaggaa aaacaatatt agtaactggc ggaactggtt ccattggaag
       61 tgaaattgtt cgtcagcttt taaaatatga accaaaagct atcagaattt atagtagagg
      121 tgaagaaaaa caattttata tgcaacaaga gctctcaaat cacactaatt tgagattttt
      181 aattggtgat atcagagact ctcaaaggat tgattatgca tgtcgtgatg tggatattat
      241 ttttcatgct gccgcaatga aacacgttcc agcaagtgaa tataatccta tggaagctgt
      301 aaaaacaaat gtgatgggaa ctcaaaatgt aatagatgca gcgatcaaaa ataatgtaaa
      361 acattttgta ggtatcagta cagataaagt agtgagtcct actaacacca tgggtgcgac
      421 aaagttattg agtgaaaaat taataatagc tgccaatcag tataaaggaa aaacagaaac
      481 aatattttct tgtgtaaggt ttggaaatgt catggggtca agtggatctg ttattcctct
      541 ttttagtaaa caaataaaaa acaataaaga tattactttg acagatgttg atatgacacg
      601 atttatgatg tcaataccgg atgctgcaag attagttctg tctgctgcta tgaattcaaa
      661 atttggagaa acatatgtct taaaaatgcc ggcaatcaat attttaactt tagctcaatc
      721 attaataaaa atatataatg atacacatga tgaaaaatat agaggtgaaa taaaagttgt
      781 aggtatcagg ccaggagaaa aattatatga agagctaatg actacagacg aaattgaacg
      841 ctcatatgaa aatgataaaa tgtatatcat tccaccacac tatcatgcta ctgacgaact
      901 gtacaaagat tatgaaaaaa ttaaaatcac tgcatactct tcagatgcaa cagaattaat
      961 gagtggacag gaatttatta gaatcgcaaa agagaataat gtcgtgactt ggtaaggagt
     1021 tttatgatga aatcagaagt atatgtaatt gcagaaattg gtgtaaacca taatggaagt
     1081 ctagatttag ctttagaaag tattgataaa gctaaagaat gtggagctaa cgcggttaaa
     1141 tttcaaacat ttaaaacgaa taagctagtt tccaaaaagg ctccaatggc aaaatatcag
     1201 aaggataatc ttaaagagga cagttctcaa tttg
//
LOCUS       DAZBSA010000230         1215 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000230 DAZBSA010000000
VERSION     DAZBSA010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.237"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            414..947
                     /locus_tag="ACHGMT_002919"
     CDS             414..947
                     /locus_tag="ACHGMT_002919"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFN6836580.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DAZBSA010000231         1206 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000231 DAZBSA010000000
VERSION     DAZBSA010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.238"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..>1206
                     /locus_tag="ACHGMT_002920"
     CDS             134..>1206
                     /locus_tag="ACHGMT_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFN6836581.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKT"
BASE COUNT          383 a          153 c          197 g          473 t
ORIGIN      
        1 tatacagcat tatctgaaga taaaaaagaa acattgacaa atattgttct tttctgtgaa
       61 cattataacc gtctatcaga agaaaaaatt ttactcccag aaaactgggc agaattaaga
      121 aagttggtag agtatgggat tatttaacac actttccaag tcaaaattag ttaagtccag
      181 tttctggtat actataggga atttttttat taagggaatt tcattcataa ctattcctat
      241 ttttgtaaat attactactc aagcagaata tggtttgatt aataatttca cttcatttgt
      301 atcaattttt tcgattcttg ttgggctaag cctaaattcg gcaattaata atgcaagttt
      361 tgactataaa gatgacataa atgaatttat gtcatcggta ctttctctag ctacactttc
      421 gttaattgtg ttcttattaa tagggaacat atattttttg ttttcaaatg ctttttttga
      481 tatgaaccaa cttgtattta atttgatgtt attacaaagt tttgctagtt ttgtaattag
      541 ttatatccag tcttatttta cgattaactt taaccatttt aaatatttga ttttatcttt
      601 tatatctact tttttgaata ttgcaatctc tgtagctttg atgcagacat tatattttga
      661 tgataaatat gttggcagag tagtcggcgg aacactgtca tttgtgttga taggtttagt
      721 aatttattta agaataatga taaagggtgc tcattttttt gataaaaaac agtggagata
      781 cgctttaaaa atctctttgc cgctaatccc acattcctta gcaaatatag ttttagcaca
      841 attcgataga attatgatta attcttattt aggtcctgct gcggggggaa tatattccta
      901 cattagtaac ataggtatta ttctaagcgt aatatggatg tctactaata acgcctgggt
      961 tccatggttc tatggtgaga tgagtaaaaa taatgaaaaa aaagtgaaga aagttagtaa
     1021 ctattattta gtagtcttta ctttgattac gatgattgta atggtagttt cgattgattt
     1081 agcaaaattt atggctccga aagactatta ttctgggtta tctttagtgg ttccgataac
     1141 cttaggctat ttctttcagt ttttatatag tttaccagtt aatgctgaat tttatgagaa
     1201 aaaaac
//
LOCUS       DAZBSA010000232         1193 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000232 DAZBSA010000000
VERSION     DAZBSA010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.239"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1076
                     /locus_tag="ACHGMT_002921"
     CDS             <1..1076
                     /locus_tag="ACHGMT_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFN6836582.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DAZBSA010000233         1172 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000233 DAZBSA010000000
VERSION     DAZBSA010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.240"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(449..>1172)
                     /locus_tag="ACHGMT_002922"
     CDS             complement(449..>1172)
                     /locus_tag="ACHGMT_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836583.1"
                     /translation="KKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNIIE
                     EIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAID
                     NVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPPT
                     WKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKKD
                     YSVVFKNGQVTDYNEVFKKVIE"
BASE COUNT          394 a          204 c          148 g          426 t
ORIGIN      
        1 cttttagaaa aagcaaaaaa aaaaagaaag aaacttattc ttgctaataa actacgcgaa
       61 aggagtctaa cgttgtttga tataacttag actgatcgtt aacttatttt ttatatttct
      121 acatagttat ctttagaacc atttcagata catatgaaac aattacatat gattttttca
      181 taaaaaaaca gtactgtcta gtagtataaa atatacaaat ttcttaaaaa ttttttcact
      241 aaaatcacta cctgaaacag tctaaaacac ttatatatca acgtttaatg aaactatctc
      301 cgtttcattt cagatacaaa taaatatagt cgggctccaa atccgaaaaa aaataccccc
      361 agtagcttat cctaagctac tggagatgta taagtacggt catataacaa cggggtaggg
      421 taaataaatg aaaaagatgg aagaatcatt attcaataac ttttttaaat acttcgttgt
      481 aatcagttac ctgtccgttc ttaaatacca cagaataatc ttttttccca ttttcaagta
      541 accgaagtgg aatatcttta ccaactttct ctgtcactgc attactgata cctgttagat
      601 tagctaataa atctgataaa gattgagcat ctgtagaaat ctctgtatca gtatttatcg
      661 tgattccttt atcttcattt acaagtttcc aagtaggtgg tatagctgtg tatgtttcta
      721 caaatgaatt aagtttttca ttaagtgact tctcttttat atttccatct aaaaactctg
      781 tatatatggc ttgagctata ctaaaacaaa gctggccttg ttctaataat tttacatcat
      841 tagttagtaa agctgtgttc acgttatcaa tcgcttcaag aagagtatct ttggtgtccc
      901 taacagcttt aggtgtgcct tctggaattt ccctacttga tacctcgtcc attttatctt
      961 ccataaaccc cttaaaatca tatctttctc ccaaatccat agaagttttt gagaaagctt
     1021 tcatttctga gtctatttct tcaataatat ttaacatgtc tgatacatat tgtttaggct
     1081 gattcgtttt taatctatat gtggtaaata tctcaattcc tccaattatc aataaaattg
     1141 ccaaacaaat ccataataat tttttctttt tc
//
LOCUS       DAZBSA010000234         1169 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000234 DAZBSA010000000
VERSION     DAZBSA010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.241"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..156
                     /locus_tag="ACHGMT_002923"
     CDS             <1..156
                     /locus_tag="ACHGMT_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFN6836584.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..>1169
                     /locus_tag="ACHGMT_002924"
     CDS             175..>1169
                     /locus_tag="ACHGMT_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6836585.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQII"
BASE COUNT          497 a           94 c          204 g          374 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaacta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc aacaagccaa
      841 gagatattaa ataattcaga aaataatgaa attgcaaata aaattttaat gtattctggt
      901 agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat tgatgagtca
      961 aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc agtgagtgga
     1021 ataaaatata taataaaagt aatgacttat tctattatgt taggtgggat ggttgttctt
     1081 tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat attcttatct
     1141 attggaacat ctaagataca aattataat
//
LOCUS       DAZBSA010000235         1136 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000235 DAZBSA010000000
VERSION     DAZBSA010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.242"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..581)
                     /locus_tag="ACHGMT_002925"
     CDS             complement(51..581)
                     /locus_tag="ACHGMT_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFN6836586.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(680..1030)
                     /locus_tag="ACHGMT_002926"
     CDS             complement(680..1030)
                     /locus_tag="ACHGMT_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFN6836587.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
BASE COUNT          410 a          205 c          181 g          340 t
ORIGIN      
        1 ttttttggat tgttggtttt tatacggtct attttaataa ctaaaaatga ttaacgtata
       61 gtcatttttc ttattctgat gaaattgata atctcctgga tgcccatgaa aatcaaaata
      121 aagccgaata tttggaatag aagcatcaaa cttttgaatg gattgaacaa taagacgaat
      181 ccgccaatca acaacagaat actactataa acactccaac ttttagcttt tgtattcatg
      241 ctgaagaaca actggaacaa gctgttgatg ataacagcta atccaagaag aatcggcagg
      301 atagaaacga ttgcaggtgc aaaagcatag acgatcagag ctaagatcag gaaaaaaaga
      361 ccactgaaca gaccgatact ccaagaacct gttttccttt taaaacggta agcctcaaaa
      421 agattcaaca acccaagcaa aacaagatag gcggtgatca gataaccgat aaaatgaaaa
      481 acagcagaag ggtttagcac aatcgcaatc ccagaaataa tataaacagc agctcttaat
      541 aaggcgtatt tttgaatatt ttgtttaatc gtctcaaaca tataagaaaa cctcctttga
      601 cttattttaa cgtgaactag tcattttacc aaattaaatg ttacaaaatg agcgaaggag
      661 aagaatatct ctggaaaaat caacttctga ggatcttttc catttttttg ccttttgcta
      721 gctcatcgat cattttatct aaataacgaa tttctttcat caagttatcc tcgatgtctt
      781 ctactcgtac accacaaaca acacctgtaa tcaaggaacg tgaaggattg gggacagggg
      841 cttccgcaaa aaattgttcg aaagtcactt gtttttgtag ttgatattct agttctgttt
      901 gggaataacc cgtcagccaa caaatgattt catctacttc ctcttttata cgatcttttc
      961 tctccgcttt tgctacatac ataggataaa cgcttgcaaa acttgtcgta aaaatacgat
     1021 tttgcgccat aatcagtcac ctttcttttt ttcaaattat ttactactta tagtatataa
     1081 gaaaaagaga agaaaaagcg attattcaaa aaaacaaaaa ggattgtgac aaaatt
//
LOCUS       DAZBSA010000236         1069 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000236 DAZBSA010000000
VERSION     DAZBSA010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.243"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..969
                     /locus_tag="ACHGMT_002927"
     CDS             <1..969
                     /locus_tag="ACHGMT_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFN6836588.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          368 a          185 c          214 g          302 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccat
//
LOCUS       DAZBSA010000237         1063 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000237 DAZBSA010000000
VERSION     DAZBSA010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.244"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..>1063)
                     /locus_tag="ACHGMT_002928"
     CDS             complement(38..>1063)
                     /locus_tag="ACHGMT_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFN6836589.1"
                     /translation="LTGNPKNIRLFLYNLFNSTEISFVELYTLPIIIKTEKMLKDLKQ
                     YYPLSAYEKKKLFHFLAINLLAEQADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFI
                     NKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFENQFNLLREETSQLIRRELTK
                     IHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFS
                     YLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPD
                     VIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPE
                     EIIDRIE"
BASE COUNT          346 a          177 c          160 g          380 t
ORIGIN      
        1 tttagaaaaa gcagtcggac tttctaaaag aagttcacta ttcgatcctg tcgatgattt
       61 cttcaggaaa atcgtctatt tgcttgtcaa ttttgctgtc tttgtatttc tcttcttgga
      121 tagtcaacaa aatctttgta agattccccc agtctactgc tcttggcgga tcgagccaaa
      181 ccactacttg agatgcactt tcttcatata gattattcct attcgtgatt ataacatccg
      241 ggaactcagg ctttcctgcc tctatcactt ccgtgttcag attaatgtac aagcttaaat
      301 ttttcttgat aaattgatta taggcagcac cataagaaaa atcgatgatg atctttactg
      361 gttcagaaat cagttgaatg ggtatgtgat tgatcaataa taataaataa ctgaaaaaaa
      421 gaaaaaattt atcttgaagc aactctttgc gttttgacgt tagatcattt atatactcta
      481 tcaaatagca ataaaatgct ggatagtttt ctctaaaaaa agaaacatca agtggaaatt
      541 catcagcttt attaatagtg taatgcaaaa gtttaaaatg tattttggtt aactctcttc
      601 ggatcaattg gctcgtctct tctcgcaaca gattaaactg attttcaaaa ctttcaatga
      661 atcgttcatt caaccattcg attgtttcat cttcattttt tctcaagtat ttttgatcca
      721 atttatcatg aagataaagc caagagagtg tcttattgat aaaaataacg gatttttctc
      781 ctaatactcc ttttaagtgg taaaatccta tatccgaaaa aaatagcgac tgattgctag
      841 caatatctaa agaatcagct tgttcagcca acaggttgat tgctaaaaag tggaataatt
      901 tctttttttc ataagcagac agaggataat actgttttaa atcttttaac attttttctg
      961 tttttataat tataggtagc gtatacaatt ccacaaaact tatctctgtt gagttaaaaa
     1021 gattatataa aaaaagccga atgtttttag gattccctgt caa
//
LOCUS       DAZBSA010000238         1062 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000238 DAZBSA010000000
VERSION     DAZBSA010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.245"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..963
                     /locus_tag="ACHGMT_002929"
     CDS             <1..963
                     /locus_tag="ACHGMT_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFN6836590.1"
                     /translation="PINGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRN
                     EAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKA
                     TFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSE
                     MGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIE
                     NANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRADVNQAYNTSGNVGYHVS
                     FNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          366 a          195 c          213 g          288 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttatcaa gtcggtcaac ctggttatgt acagtacggt
      241 gctggttcgt atgctaatgc caacagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac gttggacact ccttataacc aaccgggaat caaatcgcat
      421 ttatgggtaa cacaaaacat ctggggcgat catacagatc cttacggtta tctttctgaa
      481 atgggcgtaa gtaaagaaaa attagcatat gatttagctc atggatttac cgatgaaaat
      541 ccaacaactt ctgaaaacaa gcctgtcatt gatccaacac gagctggtgc agctaatcct
      601 acactgacag atggaacgaa ttacgcccac attgatcagt ttggagaaat cgaaaatgca
      661 aatttgcatg tagctggatg gcacattgct aactataaat acgagtatat cttcattatg
      721 gattacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagagcagat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc ca
//
LOCUS       DAZBSA010000239         1038 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000239 DAZBSA010000000
VERSION     DAZBSA010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.246"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            209..>1038
                     /locus_tag="ACHGMT_002930"
     CDS             209..>1038
                     /locus_tag="ACHGMT_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFN6836591.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          368 a          149 c          177 g          344 t
ORIGIN      
        1 agaaagttta ataagagttt ggacctagca aacaatatta atgaggtcat taaatcttat
       61 tatctttagt gatataacga aatatctaca cagacaaaat gatagcgtaa aatattttgt
      121 gtaaatagat atttagggta gaaaaaaaga aagtccattc tgtaaaatta aagttaccac
      181 aaccgaaatt ttataggagg actttctcat gcccaatttt actacagaaa ttatggaaac
      241 actaatcaat aaaggtgatt tagatgaatt atttcgatgt catttagaat tagctgttaa
      301 ttcattattg caggctgaat taacagcttt tcttgattat gaaaaatatg atcgtgctgg
      361 gttcaattca ggtaattccc gtaatgggaa ttattcacgt tcattcagaa cagagtatgg
      421 agaattaaat ttggtaattc ctagagatag aaatggtaag ttttctcaac aaactttacc
      481 tgcttataaa agaaccaatg actctttaga aaccactatt attcagctat ttcaaaaagg
      541 aattactatg tctgaaatct ctgaattgat tgaaaaaatg tacgggcatc actatacgcc
      601 acaaaccatt tcaaatatga ctaaaatagt ttctgaagat attattgcct ttaaagaaag
      661 gtccttagaa tccagatact ctgtcatttt tatggatgct actcacattc ctttaaaaag
      721 acaaactgtt tcaaaagaag cagtttatat tgtaattggc attcgattag atggaaccaa
      781 agaggttctt gggtttagtc ttgctccaac tgaatctgct tatgtttgga aagagattct
      841 tcaagaccta aaagaccgtg gtttaaaaga ggttttatta gtcgtaactg atggtttaag
      901 tggcattaac gatagtattc atagtgttta tccaaatgct caatttcaac aatgttgtgt
      961 ccatatatct agaaatatcg ctcataaggt tcgtgttagt gatcgacaag aaatctgtag
     1021 cgatttcaaa ttagttta
//
LOCUS       DAZBSA010000240         1024 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000240 DAZBSA010000000
VERSION     DAZBSA010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.247"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            258..977
                     /locus_tag="ACHGMT_002931"
     CDS             258..977
                     /locus_tag="ACHGMT_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFN6836592.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
BASE COUNT          375 a          126 c          183 g          340 t
ORIGIN      
        1 ttcaacccgc tatccacttc aagtataatt tttccatacg ccaataaagt agttttagga
       61 gcatgttctc atttgagaac gctccttttt tatatatttt gaaagctact tactatacgc
      121 gtagtttcaa atagttttgt cattctaatc ttcagcaaca gaaaaaaatc taaactataa
      181 aatttagggt tgactaaaat ataatttttg tataccatat gatcgtgata atcattatca
      241 tttagaagga gaaagttatg ttagaagtga aaaagcttaa tgtttgttat aacgattttt
      301 tagcattgaa cgagatttct ttaaaaatac aagaaggtgc tttaacaggt attattggtc
      361 caaatggtgc tggaaaatcc acattgctta aagcaatgtt aaatattatc cctcatcagg
      421 gagaggtctc cattaataat gaagatatca ataaaaaact ggtgaagatt gcatatgtag
      481 agcaaaaagc tgatattgat tttacttttc caataaaagt aaaagaatgt gtatctatgg
      541 gaacatatgc cggaatgaaa gtttttcaaa gaattaaaaa tgcagaatgg caaagagttt
      601 caaaggcttt ggaaaaggtc gatatgggaa aatattctaa tcatcaaata ggagaattat
      661 ctggaggaca atttcaacgt gtacttttag caagatgtct tgctcaaaat gcggatttca
      721 tatttttaga tgaaccattt gtagggatag acttagttag tgaacgaata attatggata
      781 cgttaaaaaa attgaaacat cagggaaaaa cagtgctaat tgtgcatcat gatttaagca
      841 aggtaaagaa gtattttgat aatattattc ttttgaatcg aaatctaatt gctcacggaa
      901 gtgtagaatc tgtatttaat gaagaaaatt taaaaaaagc ctatggagat actatattta
      961 ttggagaggg ggaataatat gcttcaaaat tttattaatg gtctgtatga ctttcacttt
     1021 ttac
//
LOCUS       DAZBSA010000241         1022 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000241 DAZBSA010000000
VERSION     DAZBSA010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.248"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..461
                     /locus_tag="ACHGMT_002932"
     CDS             3..461
                     /locus_tag="ACHGMT_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFN6836593.1"
                     /translation="MLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTTLFVSV
                     IYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGLLPKFM
                     KVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            470..697
                     /locus_tag="ACHGMT_002933"
     CDS             470..697
                     /locus_tag="ACHGMT_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6836594.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          319 a          167 c          180 g          356 t
ORIGIN      
        1 cattgctatt aaaatttgtc gttttaggta taagtgccgt atttattttg ttcggcgtac
       61 ttttttatac tcagatcgct acaagcgaaa aaggattcaa ttttgattgg cagaccacat
      121 tatttgtttc tgtcatttac tttgcccttc tcctagggat cgttattgct tatttcttat
      181 tgaaactatt gaaaaaaatc gaagataacc aagctttttc cactcaaagc ttaaacatac
      241 taaaaaaaat cgaacgaacc attttgttta ttggtttcgc cttttttggc ttacttccta
      301 aatttatgaa agtagcggat ttagacgatg ctccaggttt gatgttggta ggaatcgctg
      361 tcgtattcat tccttttgca gtatacacat taacaagcgt gctggaaaaa ctgcttgaac
      421 atgcgatttt acttaagaca gatcatgatt tgacagtgtg aggtgcgaca tggcaattat
      481 cgttaattta gatgtagtat tagctcaaaa aaaaatgagt gtgactgaat tgagtgagaa
      541 agtgggcatt acgatggcaa acatctctat tctaaaaaat ggaaaagcaa aagccatccg
      601 tttttccact ctcgaaaaaa tctgccaagt tttggagtgt caaccaggtg atttgttgac
      661 ctataaaaat gactcgaata ctataccaga agaataaaat aaagtagaac aaacagtaaa
      721 taaaaaataa tctgaatgat agatgtgtga gacttttaga agttatttac gtctattatt
      781 cagatttttt atttcgattg aataccgttt tatctataaa taagatatgt actgactcat
      841 acaaaggggg ccttttcttc tccatgtact atcacttttc ctgatacaac tggtttcctg
      901 ttacgttgta gttggatctg cttttagtat aatactttat tcattccacc agactgatag
      961 cagtttgagc agtctaaata atcatgtagt attacaggcc attctgtttc ataccttgaa
     1021 gt
//
LOCUS       DAZBSA010000242         1014 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000242 DAZBSA010000000
VERSION     DAZBSA010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.249"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1014
                     /locus_tag="ACHGMT_002934"
     CDS             <1..>1014
                     /locus_tag="ACHGMT_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836595.1"
                     /translation="MNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAFHINR
                     ALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYIVTL
                     ITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLILLG
                     FIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFLIIK
                     LIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLS
                     FGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQA
                     PWRF"
BASE COUNT          324 a          138 c          138 g          414 t
ORIGIN      
        1 atgaatttga aaaaaatttt ttctgtaaaa atatttatag tttatgcatt tgttagttta
       61 ttatatttat ttccagcatt taaaaacatg gaaatatttg gcttttatga tacggcattt
      121 cacattaatc gtgcgttaag tttagaatct atttttagct cccctattaa ttttgaaacg
      181 tttagatcat atggcatgca agtaaataat ttttacccat ggttaactct atatccttta
      241 tttttattga tcaagtttac taatttagca ataggctata acctttttct ttatattgtt
      301 acgctcatta ctctatttat atgtcattat gtaatgtatg aaattaccaa aaaacatgtt
      361 actagctcct ttttcgctat tatatataca acatcttctt ttaggagtgt agaaatattt
      421 cttcgagggg caatggggga attactcgca atgtcaatac ttcctcttat tttactcggg
      481 tttataaagc tttacgactc aaaaaaagaa tcttgggtta tgttggcgat tagcatgact
      541 ttattaattt acactcatgt tctttctgta gctatgacta tggtaatgat tataattgct
      601 ttaattattc aattttatcg gaaaaagaaa atagaaatct tcttaattat taaattaata
      661 aaagctgcta tagttacttt acttctttca ctagcgttta taggtcctat gattgagcag
      721 acattgtatc aagatctgaa tagaccttat gtggatatat tacaaaaaag agcaatttat
      781 ctgggtaaag agtttttgat tggaagtata gattcagaat tactatcatt tggtattggc
      841 ttaactttgc ttatttgttt aattatacta acatttaact ttaaaaaatt aggaatctta
      901 tctaaaatca cttatataat gggtataata tctattctac tttgtacaaa aatatttcca
      961 tggtttgcct tacaaaatac tcctttaaat attattcaag ctccttggcg tttt
//
LOCUS       DAZBSA010000243         1006 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000243 DAZBSA010000000
VERSION     DAZBSA010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.250"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..231)
                     /locus_tag="ACHGMT_002935"
     CDS             complement(<1..231)
                     /locus_tag="ACHGMT_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836596.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTN"
     gene            complement(233..>1006)
                     /locus_tag="ACHGMT_002936"
     CDS             complement(233..>1006)
                     /locus_tag="ACHGMT_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFN6836597.1"
                     /translation="FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIK
                     WMVSILLVLLVYFSFGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHS
                     SDNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKR
                     AHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILS
                     IFIDEMVEAHVLFNKRDVISIVFWYYLGSISYLYLKKEN"
BASE COUNT          384 a          174 c          121 g          327 t
ORIGIN      
        1 gttagttaac aagttaattt tacgtttaaa ggaagaaccg attttccggt gattcacaaa
       61 aacaacatca ttaaatagcc ctaattcttt tatacgctca ctatatcttc tagcgttttc
      121 aaattcagaa taaataaata aatctccttt tgattctcgg taaaaatttt gataaacatt
      181 taatatatta atcaaatgta aaggagtcca agcaatgaaa gctgtcttca tattaattct
      241 ccttttttaa atacaagtaa gaaatgcttc ccaaataata ccagaaaaca attgaaatga
      301 catcgcgctt attgaaaagt acatgagcct ccaccatttc atctataaaa atagatagta
      361 taaaaattaa aacaatacca agaaacgcat aattcacttt acttctaaaa aatgttttga
      421 gtaaactaaa gatatttaac acatagaaag catagataca tataaaagca attataccac
      481 ttgatacaag tacttgaaga aatccattat gcatgtttcc atgtgctctt tttagttcat
      541 tcacatctgt agtatcaaga tctacttttt tcaaaaattt tgcatcgcta ttcccataga
      601 aatcggtctc tccataacca aataacaaag ttggtcttat aactttaagc gcagctttcc
      661 aaatcgtaaa acgaccagct gaaatttcgg agttatcact agaatgctgt aatattactt
      721 ctttttctgg aagtggagtt ttatctgatt catttgactc tataacttct acttttctat
      781 caatcgatac aggaattgtt gctaaaaaat ccttagcata attagtacca aaagaaaaat
      841 ataccaatag aactaataat atgcttacca tccatttgat ttttacaggt agtacttctc
      901 taatgctctt tctaaatttt gaattggcaa acaacaaaag aaataatact aaaaaagaac
      961 aaattactac ctgggcacct ctagacgaag aaagtgtaat ataaaa
//
LOCUS       DAZBSA010000244         1002 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000244 DAZBSA010000000
VERSION     DAZBSA010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.251"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            369..1002
                     /locus_tag="ACHGMT_002937"
                     /pseudo
     CDS             369..1002
                     /locus_tag="ACHGMT_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          183 g          295 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac ct
//
LOCUS       DAZBSA010000245          990 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000245 DAZBSA010000000
VERSION     DAZBSA010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.252"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            56..466
                     /locus_tag="ACHGMT_002938"
     CDS             56..466
                     /locus_tag="ACHGMT_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFN6836598.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            480..836
                     /locus_tag="ACHGMT_002939"
     CDS             480..836
                     /locus_tag="ACHGMT_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFN6836599.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          339 a          153 c          195 g          303 t
ORIGIN      
        1 gtattttaaa agaaattact gtagaattgg attataatta aaaaggagtg agaaaatgat
       61 aaaagtatat acttcgccta gctgtacttc ctgtagaaaa gcaagagcat ggttgcaagc
      121 gaatcaactt gaatttgaag agaagaatat ttttgccgaa ccgttaacag aaagtgagat
      181 caaaaagatc ttggcagcga ctgaaggagg agttcaagat attatttcca ctcggtcgaa
      241 aatttacgaa aagctgggca tagattttaa cgaattaact ttaaagcaaa tgatcgtctt
      301 attcaaagaa tatccttcat tattgagacg acctattttg attgatgaaa aaagaatact
      361 aatcggattt aatgaagatg agatccggtc atttgtccct cgtgaaattc gatgtgttgc
      421 cagaaatctg atctatttac aaaacaataa catcttaaat tcatagggag ggagccttcg
      481 tggaagtgaa agtctatgga tcagcgcact gtgtaacaag tctgaaggcg aaacaatggt
      541 tagaacacca tcaattgcct tatcaatttg ttgacttaga agaacgcgat ttaagtacag
      601 aagaagcaga agaattatgc agttttgaag aagtgcaagc agaacagcta tttgcgactt
      661 ggtctgaggc ttttcacaac attgcgattg acctttcctg tgtaggaaaa aaacaactga
      721 tttttttatg cacacaacat accaatctcc tcagacgtcc tttgattatt atcgatgaag
      781 ttctatttat tggatacaat gagcagttga tggaacagct tatcttaaat aaatagactt
      841 taactgtttt aatcaagtca ctgagcattt catacagttg taacagtcga acacttagca
      901 tcaagcgtgt tatgtgttct ttttttgact ttatttttct actatagaga catgctcaat
      961 ttatgtggtc aaaattcaaa tctaggtggg
//
LOCUS       DAZBSA010000246          988 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000246 DAZBSA010000000
VERSION     DAZBSA010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.253"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..151)
                     /locus_tag="ACHGMT_002940"
     CDS             complement(<1..151)
                     /locus_tag="ACHGMT_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFN6836600.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLT"
     gene            845..>988
                     /locus_tag="ACHGMT_002941"
     CDS             845..>988
                     /locus_tag="ACHGMT_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFN6836601.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLF"
BASE COUNT          360 a          160 c          123 g          345 t
ORIGIN      
        1 cagttagtaa ctttttataa tcataaaaaa aataagtagt tctatctgag cgatactctt
       61 ttataatact gaattcggaa ttaaaaacca cgataggtaa ctttaaacaa acatgaagag
      121 actttaataa gacttcttta tctgaaaaca cttttgtttc tctctttcct ttctacatca
      181 ttaaatagta ttttatacgt tatctaaaat actattcctc tgtcaaaata agctaactta
      241 cttcattata aaatatccac ttatcgcttt catgtatatt ccaccgtaaa aagtacattt
      301 tatcaccgag aactcatcat ttttcatcat gatgaatact atttacatat atctagttca
      361 aagacaataa ttattatgat ccgttattat ctaagcacta tacagataat aggaattttt
      421 cgtttctaaa tattccaatt tgggaatcca ataaaaattt tcgttcaaat acgtgtctat
      481 tcgaactctt ttctagttgt taattatgaa gggggaagaa ataaaaaata caatctatac
      541 acgaataaga atattatcca aaaggaggtt ttagaaaaga tttattatac gtaaaaagct
      601 caagacattt tcttagccat aaggacatgt ttgatattta agaagatata tgaattttct
      661 tttctaagta taaagataac tatccattat caactaaaaa aacatttttg aaaataaaca
      721 tcagcagtcc tatcaatgca tgtgagctta tagaagttat tcgcagcgca gcataattgc
      781 attagcttct tttcgtgttt tttgcctctt ttccctttaa atcttaatgg aggaggtgtg
      841 caatatgtca gataacgacg aacgcgtaaa tgcccagttg attcgattct ttgaaaaggt
      901 cattttgaac accgctaata cttattataa aaaacaagaa gaaattcaaa aacatgaaca
      961 acttgatcaa ctcccaccat atttgttt
//
LOCUS       DAZBSA010000247          986 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000247 DAZBSA010000000
VERSION     DAZBSA010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.254"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>986
                     /locus_tag="ACHGMT_002942"
     CDS             <1..>986
                     /locus_tag="ACHGMT_002942"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFN6836602.1"
                     /translation="LEPGDFIMSGAGTIGRISRVPKQIKPGVFNQALIRLRINKEITD
                     SEYFIQFIRADFMQRKLTGANPGSAITNLVPMSEVKKWIVQFPILEEQKKIGNFFKQL
                     DDTIALQQRKLDLLKETKKGFLQKMFPKNGAKVPEIRFPGFTEDWEQRKFKEFSKKTG
                     KKNTKDLDFPAYSVSNKAGLISQTEQFDGSRLDDLEKTNYKFVEPNEFAYNPARVNVG
                     SIAFNNLGMTVIVSSLYVVVKMSEDLDNEFILQFIKSPTFIKEVKRNTEGSVREYLFY
                     ENFANIKFPFTRNKEEQQKIGAFFKQLDDTIALHQRKLDLLKETKKGFLQKM"
BASE COUNT          375 a          159 c          189 g          263 t
ORIGIN      
        1 ttagaaccag gcgattttat catgagtggc gctggaacaa ttgggagaat ttcaagagta
       61 ccaaagcaaa taaaaccggg tgtattcaat caggctttga ttagacttag aataaataaa
      121 gaaatcacag attcagaata ttttattcaa tttattcgtg cagattttat gcaaagaaaa
      181 ctcacaggag ctaatccagg ttcagcaatt acaaatctcg taccaatgtc agaagtgaaa
      241 aaatggatag tacaatttcc aatattagaa gaacaaaaga aaattgggaa cttcttcaaa
      301 caactggacg acactatcgc tcttcaacaa cgtaagctag atttattaaa ggaaacgaaa
      361 aaaggattct tacaaaaaat gttccctaaa aacggagcaa aagtgccaga aattcggttt
      421 cctggattta cggaagattg ggaacagcgt aagttcaaag agtttagtaa gaagactgga
      481 aaaaagaaca cgaaagattt agacttccct gcctattcgg taagtaataa agcaggttta
      541 atttctcaaa cagaacagtt tgatggtagt aggttggatg atttagaaaa aacaaattat
      601 aaattcgttg aaccaaatga attcgcctat aatccagcaa gagtaaacgt aggatcaatt
      661 gcatttaaca atcttggtat gacggtcatt gttagctctc tttatgtagt tgtgaaaatg
      721 agcgaggact tagataatga gttcatactt cagtttatca agtcaccaac atttatcaaa
      781 gaggtcaagc gcaacactga aggaagcgta cgagaatact tattctatga gaatttcgct
      841 aatatcaagt ttccttttac taggaataaa gaggaacaac aaaaaatcgg cgctttcttc
      901 aaacaactcg acgacacgat cgctcttcat caacgtaagt tagatttgct gaaggaaacg
      961 aaaaaaggct tcttacaaaa gatgtt
//
LOCUS       DAZBSA010000248          985 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000248 DAZBSA010000000
VERSION     DAZBSA010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.255"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..400
                     /locus_tag="ACHGMT_002943"
     CDS             <1..400
                     /locus_tag="ACHGMT_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFN6836603.1"
                     /translation="VVMISWLLVSVYTSNELLSSLEKIHLPKKIMLSITVVIRFFPTY
                     RSEIRMIKESLKMRNIRLSFFQPLQYLEFLLVPVLIRATMIAEEMTANAITKGIESPV
                     KRSSYYKTNMSLLDYIILFSVTIFFLSILI"
     gene            411..>985
                     /locus_tag="ACHGMT_002944"
     CDS             411..>985
                     /locus_tag="ACHGMT_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN6836604.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKG"
BASE COUNT          344 a          135 c          167 g          339 t
ORIGIN      
        1 ggtcgtaatg atctcatggt tgttggttag tgtctatact agcaatgaat tattatcctc
       61 tttagaaaaa atacatttac cgaaaaaaat aatgttaagt attacagtag tgatcagatt
      121 ctttccgact tatcgttctg aaataagaat gattaaagaa tctttaaaaa tgagaaacat
      181 tcgcttgtct ttttttcagc ctctgcaata tttggaattt ctgttagttc ccgttcttat
      241 acgtgcaact atgattgctg aagaaatgac cgctaatgca ataacaaagg ggatagaatc
      301 tcctgtgaaa agaagttctt actataaaac aaacatgagc ttattagact atatcatcct
      361 gttttccgtt actatttttt tcttaagtat tttgatttga ggtgaaagaa ttgtttgaat
      421 ttaacaatgt ttctttttca tataaggatg ttaaggaaca agcgcttgtt gaccttaatt
      481 tttcaataaa aaaaggcagt cttacattga ttactggttc ttctgggagt gggaaatcca
      541 cactattaaa gttaatgaat ggacttattc caagacttta tgacggaaaa ttggttgggg
      601 aaattttatt tgaaaataac tcgctacaat ccttaacaca agaaagtata tcaaaaaata
      661 ttggctacgt aagtcaagat cctagaggac aattctttac aacaaataca acgagtgagt
      721 tagtattttc gatggaaaac tttggattta caaaagagca tatggataaa agaatcaatg
      781 aattagctga attattagat ctttcaaata tattagataa aaacattttt tcgctatcaa
      841 gtggtgaacg gcagaaaatt tctattggat gtagtttatc gctaaactct aaattaatta
      901 tattggatga accgtcttca aatttggact tcaaagcgac taagaaactt gctgacttac
      961 tttgtgattt aaaaaataaa ggatt
//
LOCUS       DAZBSA010000249          977 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000249 DAZBSA010000000
VERSION     DAZBSA010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.256"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..715
                     /locus_tag="ACHGMT_002945"
     CDS             23..715
                     /locus_tag="ACHGMT_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFN6836605.1"
                     /translation="MNKKIVVSGLIIGGLVLAGIAGKNFADDILLWGGEDNIEQINHN
                     LESLDKALENKEQNISSLNSRLSSNNQELEQAKANVEEYKQKVLTLENDKNQLTIEKT
                     NLENQLSGKNAELQGKQNEINAKIDEINQKIAEINQLNTDWNNRLQTEQNKFNDAQNT
                     VNSLNQQINQLSNEKNTIKNQLQSTEQKLEKAQSENSNLNNYIEKLEKAKSDVEDTAN
                     RSQEIVTEHTGK"
BASE COUNT          436 a          118 c          157 g          266 t
ORIGIN      
        1 ttatgttaag gagaatgatg atatgaataa gaaaattgta gtaagtggat taattatagg
       61 tggattagtg ttagcaggga tcgcagggaa aaactttgcc gatgacattt tgttatgggg
      121 tggagaagat aatattgaac aaatcaatca taatttggag tcattagata aagcactaga
      181 aaataaagag caaaatatta gtagtttaaa ttcacgatta tcttcaaata atcaagaact
      241 tgagcaagca aaggctaacg ttgaagaata taaacaaaaa gtgttgactt tggaaaatga
      301 taaaaatcaa ttaactatag agaaaacaaa tttagagaat caattatcag gaaaaaatgc
      361 tgaattgcaa ggtaaacaaa atgaaatcaa tgctaaaatt gatgaaataa atcaaaaaat
      421 agctgaaata aatcaattaa acactgattg gaataatcgt ttacaaacag aacaaaataa
      481 atttaatgat gctcaaaata ctgttaatag tttaaatcaa caaattaatc aattgtcaaa
      541 tgaaaaaaat actattaaaa accaattgca aagtactgaa caaaaactag aaaaagcgca
      601 atcagaaaac agtaatttga ataattatat tgaaaaactg gaaaaagcca aaagcgatgt
      661 agaagacaca gcaaacagat cacaagaaat cgtgactgaa catacaggaa aatagatgca
      721 ttttttagtt taaatttctt taacaaaata gtattttgtt gctaaataat ttttatttga
      781 atattctaca atgatatctc ccctcaaaat ggaggggaag tacatactgt gataatctac
      841 aggtttattg actgattagg atgttattaa aaaataaaat actaaaatgc atacccgaat
      901 ttcatacccg aaaaataaaa aatgccacca cgtacactct tttcgaaact acctaagtag
      961 ccaagaatgt actggtg
//
LOCUS       DAZBSA010000250          943 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000250 DAZBSA010000000
VERSION     DAZBSA010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.257"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>943
                     /locus_tag="ACHGMT_002946"
     rRNA            <1..>943
                     /locus_tag="ACHGMT_002946"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAZBSA010000251          937 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000251 DAZBSA010000000
VERSION     DAZBSA010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.258"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..616
                     /locus_tag="ACHGMT_002947"
     CDS             <1..616
                     /locus_tag="ACHGMT_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HFN6836606.1"
                     /translation="VSSLLYFGIPSRLREDILIFVTKENDVDLNGEKSGISIEGTSTV
                     QTILEPSNSVSRKGKNTRNGERSKRDYDNRNSANDDAGETAEQIAYAELTQNQKLNVI
                     WNTKYSSKSSDRNKQPPNGIVCDMWVHDPENGNMYFEVKSSITEFEMSINEYNSMLDN
                     PDSYEVVLVNRDTKEISRHKFDELDGLKQASSYLFKFKQKRSDG"
     gene            751..879
                     /locus_tag="ACHGMT_002948"
     CDS             751..879
                     /locus_tag="ACHGMT_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFN6836607.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT          361 a          136 c          176 g          264 t
ORIGIN      
        1 agtttcttcc ttactgtatt ttggtattcc gtcccgttta agagaagata tactgatatt
       61 cgttactaaa gaaaatgatg ttgatttgaa cggagaaaaa tctggtatct ctattgaagg
      121 aacatcaact gttcaaacca ttcttgaacc atcgaatagt gttagtagga aaggtaagaa
      181 cacacgaaat ggagaaagaa gtaaacgaga ttatgataat agaaactccg caaatgatga
      241 tgctggtgaa acagcagaac aaattgctta cgctgaattg actcagaatc aaaaacttaa
      301 tgtaatttgg aatacgaaat attctagtaa atcttcagat cgtaataagc agccaccaaa
      361 cggaattgtt tgtgatatgt gggttcatga cccagagaat gggaatatgt actttgaggt
      421 aaaatcatca attacagaat ttgaaatgtc gattaatgaa tataattcta tgttagataa
      481 ccctgactct tacgaggtcg ttttagtcaa tcgtgatacc aaagagataa gtcgtcataa
      541 atttgatgaa cttgatggat tgaaacaggc aagtagctat cttttcaaat ttaagcaaaa
      601 gagaagtgac ggttgagtga tttgtttcca caaacctatt caatctatca actcaaaaat
      661 cataaacaac cattattata gtaggaagat acagagtgta ctctcaacgc atatcgaatt
      721 agtcgcttca ttcgttaaag gagaataaaa atggaccaaa aagaaaaaat gaaagaattg
      781 atcaaaaaga aacaaggtgg aggccgatcc aaacaaatgg aaacacctaa gaatgatcgt
      841 aaaaacatga gaaaaggccc taaaatcttt aataaataga cgtgctaaag aaagactaag
      901 aagtgttaac ttttgttatt gtttcttagt cttttta
//
LOCUS       DAZBSA010000252          922 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000252 DAZBSA010000000
VERSION     DAZBSA010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.259"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>922
                     /locus_tag="ACHGMT_002949"
     CDS             <1..>922
                     /locus_tag="ACHGMT_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFN6836608.1"
                     /translation="KSCQRTFVADTSVAEKHCFISQKVRWSVIARLKENTSMTEIARQ
                     KNISTSSVYRVMKRFYRPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALL
                     DIVENRRLPYLERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIV
                     QHIGRTFLNHRVKETTVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASF
                     KDYLTESEIADRLLAACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYT
                     TTLRTFKKFLPQIQNALHYSYSNGPLECLNN"
BASE COUNT          280 a          187 c          183 g          272 t
ORIGIN      
        1 taagtcttgc caaagaacat tcgtagcgga tacctctgtt gctgaaaaac actgtttcat
       61 tagtcaaaaa gttcgttggt ctgtcattgc tcgcttaaaa gagaatactt ccatgacgga
      121 aatcgctcgt cagaaaaata tttccacttc ttcggtctat cgtgtcatga agcgattcta
      181 ccgcccattg aatcctttca aacagacttt gccaaaggtc ttatgctttg atgaattcaa
      241 atcggttcga agtgttgcca gtgcaatgag ttttatcatg atggacggac aaagccatgc
      301 attattggat attgtcgaaa atcgacgctt gccttattta gaacgctatt tttctcggtt
      361 ttcattagcg actcgtgaag ccgttcaatt gattgttatt gatatgtatg cgccttatgt
      421 ttcgttggtg aaaaagcttt tcccgaatgc tcagttgatc atcgatcgtt tccacatcgt
      481 ccaacatatt ggcagaactt tcttgaatca tcgagtgaaa gaaaccacgg tacgacttaa
      541 aggaaattcc cactctcaac gagggttagg gaaaaaattg aagcgctatt ggaaactcct
      601 acaaaaagaa gaagcaaagt taaactatga aaaacgggtt tggcgtgcca gttttaaaga
      661 ttatttgact gaaagtgaga ttgccgatcg actgttagct gcttgtccca acttacgcca
      721 aggctatcag ttataccaag acattcttta tgcggtaaag aaaagagatc agtcactatt
      781 tgaggattgt ttaactagag acattagcgg tctgccagaa acctatacaa ccactttacg
      841 aacgtttaaa aagtttctgc cacagatcca aaatgcgctt cactacagct attcaaacgg
      901 accattggaa tgcttaaaca ac
//
LOCUS       DAZBSA010000253          902 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000253 DAZBSA010000000
VERSION     DAZBSA010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.261"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            44..736
                     /locus_tag="ACHGMT_002950"
     CDS             44..736
                     /locus_tag="ACHGMT_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFN6836609.1"
                     /translation="MRKKIIINAGVIIGLMLVIFSGKQYADNILLWGGEDNIEKINNN
                     LTILDTALENKEQKISILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKT
                     NLENQLSAKDSELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQST
                     INSLNQQINQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTAN
                     KAQQIVNEHTIQ"
BASE COUNT          404 a          106 c          134 g          258 t
ORIGIN      
        1 ttaataactt atttaaggaa attttttgaa ggagaagata aaagtgagaa aaaagattat
       61 tattaatgct ggagtaatta taggattgat gttagtaatt ttctccggaa agcaatatgc
      121 tgataatatt ttattatggg gaggggaaga taatattgaa aagatcaaca acaatttaac
      181 tatattagac acagctctag aaaataaaga gcaaaaaatt agtattttaa attcacaatt
      241 gtcttcaaac aaccaaactc ttgaacaact aagaactaat attgaagttt ccaaacaaaa
      301 agtggcatct ttagaaaacg acaagaatca attagtatca gagaaaacta atttagaaaa
      361 tcaattgtca gcaaaagact ctgaattgca aggaaaacaa aatgaaatta atgcgaaaat
      421 agacgaaatt aaccaaaaag tagctgaaat aaatcgatta aatagcgatt ggaataatcg
      481 tttacaaaat gaaaagaaca aatttaatga agaacaatct actattaata gtttaaacca
      541 gcaaattaat caaatgttga gtgaaaaaaa taacattcaa aatcaactgc aagctactca
      601 gcaaaagcta gaagaagaaa agtcaaaaaa tagtagtttg aataattaca ttgaaaagct
      661 tcaaaaagct aaaaatgatg tagaggatac cgcaaataaa gcacaacaaa ttgtaaatga
      721 acatacaatc caatagatat agttttaatt taacaaatgg tatgtttgga gataatgaga
      781 tagaatataa atgtcataat attatacaat ctaaacaccg tttccttttc tatttttgat
      841 gacatcagta tatagctact cgaaagagta gtctttttat tattctcgct tattattgat
      901 at
//
LOCUS       DAZBSA010000254          893 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000254 DAZBSA010000000
VERSION     DAZBSA010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.262"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            141..398
                     /locus_tag="ACHGMT_002951"
     CDS             141..398
                     /locus_tag="ACHGMT_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836610.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(630..>893)
                     /locus_tag="ACHGMT_002952"
     CDS             complement(630..>893)
                     /locus_tag="ACHGMT_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HFN6836611.1"
                     /translation="EHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEK
                     EKLLYITKTSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          344 a          111 c          121 g          317 t
ORIGIN      
        1 ttctgtccac gttcatatta ctccaaagtt ttcctcattc caaaagaaaa gcacagaata
       61 cacaaagaag cgtttttatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttgt tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaataaat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 tttacatttt tttaggaatg taaagtctta tctcttttct taattgtaca attagtatta
      601 tctgcactac tttctaatta aataaatttt tagttattaa cttgattaaa atcagagata
      661 atatcattta attcagtttg aagggttgta acgattaaat ggaaattttc ttcatctgtt
      721 ttattatatg gggatgtttt cgttatatat aacaactttt ctttttcaat tttcattaat
      781 aactctgtcg aagtctgtac tttatcttga taattaaatg cagacatccc actttgtata
      841 attgtaatta ttgctgaagc aattgaaaca atcaatagta aagacctgtg ttc
//
LOCUS       DAZBSA010000255          885 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000255 DAZBSA010000000
VERSION     DAZBSA010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.263"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(673..858)
                     /locus_tag="ACHGMT_002953"
     CDS             complement(673..858)
                     /locus_tag="ACHGMT_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836612.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
BASE COUNT          324 a          174 c          109 g          278 t
ORIGIN      
        1 aaatgcttca agaagtccaa gaaataaagc cgtataaaaa caagaaccta tgaaatcttt
       61 atttattaga tattcaacag aaatcccgat aacactagcc aacaatatat ttatcagaag
      121 tgtaaaataa ggatgctttt tatagaatag aactagtgtt gttttcatag actctccttt
      181 cttcatattt ccaattaatc acaaattcac ggcattacta gaaaaattaa aggggcgcag
      241 aagtggaaaa atactcttcg actttcccta caaaccaggc catcttgtgt ttaataacta
      301 taccaaatct aaggtacctc taaatctaaa ttccatttgt ggcggtgacc tccaattttt
      361 aactcttcta tctcccatcc agtatacata accttgatac ttcgaccact tatatatcga
      421 acacacaaat acctatccat tgcaggagta ctacctaact tagttgctac ttgcatctaa
      481 ataacagctg aaaagatagg acttactctt agtaaagtat ctgaactatt aatttttaaa
      541 ctatcatttt tatcctcaac tacatgcaca tctgttgcaa cttcactttc tgtctcatcc
      601 agataaatga ttaaggaatc gggaattgca atatcttcag ctgacgctgg aatagttgag
      661 ctaaattagt catcatatct ttattataaa ataaccaaca gcaaaaaata caatgaaaaa
      721 caaacttgga actaatgtat cattaataaa tgttgaaaaa ttcagcaact cattactgta
      781 tgattttagc agcccaacca acatataccc tataaaagct aataagatgg ttaggaatcc
      841 tttagaattt ttactcattc atagacactt ccttaaataa ctttt
//
LOCUS       DAZBSA010000256          879 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000256 DAZBSA010000000
VERSION     DAZBSA010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.264"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..817
                     /locus_tag="ACHGMT_002954"
     CDS             <1..817
                     /locus_tag="ACHGMT_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFN6836613.1"
                     /translation="QVYFGGGINLWLAKKNGIRQRIAHSHATEDGKSQNIVMNVLRKF
                     LTKLLLQNATDYLAVSQEAGESLFQNHPFEIVHNGIDLELYSKNSEAKVNKRKELDIS
                     MSTFVVGNIGRLEKQKNQSYLLEIFLEILKMNSDSYLILVGEGSLRNKLKAKAVELDI
                     EKRVKFLGERNDIPELLSVMDVFVMPSLYEGLPMVGLEAQAAKLKLVLADTISKDTKL
                     TNNVDFVSLDETPQEWAKKVIKEPYANKITQELKSYDVSYTTEQMESIYSRDH"
BASE COUNT          337 a          101 c          171 g          270 t
ORIGIN      
        1 tcaggtttat tttggcgggg gaattaattt atggttagcc aaaaagaatg gaatcagaca
       61 gagaattgct catagccatg caactgaaga tggaaaatct caaaatattg taatgaatgt
      121 attgcgtaaa tttttaacca aattgttgtt acaaaatgca acagactacc ttgccgttag
      181 tcaagaagct ggtgaaagtc tgtttcaaaa ccaccccttt gagatagttc ataatggtat
      241 tgatttagag ctttatagta aaaattcaga agctaaggtt aataagagaa aagaattgga
      301 tatctcaatg agtacttttg ttgtgggaaa cattggacga ttagagaaac aaaaaaatca
      361 atcttatttg ttagagattt ttttagaaat actaaaaatg aattctgatt cttacttgat
      421 tttagtaggt gaagggtcac ttagaaataa actgaaagcc aaagcagtag aattggatat
      481 agagaaaaga gtaaaatttt taggagaaag aaacgatatt cccgagttat tatctgtgat
      541 ggatgttttt gtaatgcctt ctctttatga aggtctgcca atggttggat tagaagcaca
      601 agcagcaaaa ttaaaattag tcttagcaga tactatttcc aaagatacaa agttaacaaa
      661 taatgtggat tttgtatcat tagatgaaac accacaagaa tgggcaaaaa aagtaataaa
      721 agagccgtat gctaataaaa taacacaaga actaaagagt tatgatgttt cgtacactac
      781 tgaacaaatg gagagtatat atagtagaga tcattaaaaa tgttcaagat attttaggag
      841 tgattatttt tgaaaatatg ttatttgata ttggcatac
//
LOCUS       DAZBSA010000257          864 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000257 DAZBSA010000000
VERSION     DAZBSA010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.265"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(19..723)
                     /locus_tag="ACHGMT_002955"
     CDS             complement(19..723)
                     /locus_tag="ACHGMT_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836614.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT          244 a          161 c          150 g          309 t
ORIGIN      
        1 ttatgacaaa tagaattttt atcgatcagt aatcgctgtc gctgcaaatt gttcgatcaa
       61 aggaatcaaa gtcaatttga ctttgtattt ccgttcgagt gctttttcga taacgaactc
      121 attttcagta gtcatagaaa tagttgctaa tttacgaggt tcatcagcat ttttttcatt
      181 aataactact tcatgttcat tttgttctgt gtaaaattta ggatactcag caacggcttt
      241 tgaaaaatca gcaaaactta aagcggcatt cgtatcagtt gagcgtgtat tttcagttgt
      301 gtccgctttt ggtttttccg ttttttcttc tggcacactg actgtttctt ctgtaactgg
      361 tgtcgcaaca gcttgcgcag ttggttcatc gtttttcaat cgttgatcat tccagtaaga
      421 cacgatttca ataagaagat tgtcacggaa tcgttcgaaa atcccgtttt ctcctgaacg
      481 aattctcttt aatacagtat tgaagttgtc atcgattggc acgttgatcc gtgtatcttc
      541 atttgttttc ccagctaatc cgatcgttaa gatttgacgg tttttgggat caagcatgac
      601 ttgtaacggc aaaccataac gtcgcaagct ctcttcttct agagaacgga ataattgtcc
      661 tcttacacca tccaatgaag gacggaattg atttaataat ttttgtttct gttttttctt
      721 catatgttct ctccttcgct catacccttt cgattataag aaaaaaacag ccaaatgaaa
      781 aggagtaatg caaaaaaact tgcgtatacg caaattacaa cagaaaaatt tgcgtatttt
      841 cccgtttttt gaaagaaaaa taaa
//
LOCUS       DAZBSA010000258          843 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000258 DAZBSA010000000
VERSION     DAZBSA010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.266"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>843)
                     /locus_tag="ACHGMT_002956"
     rRNA            complement(<1..>843)
                     /locus_tag="ACHGMT_002956"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          184 a          259 c          187 g          213 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gct
//
LOCUS       DAZBSA010000259          838 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000259 DAZBSA010000000
VERSION     DAZBSA010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.267"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          244 a          133 c          103 g          358 t
ORIGIN      
        1 tttacttccc actgaaatgg gaagtatatt ttaaatattt tttctccttc aacttacact
       61 ataaagtatc ttaatcacac gatatgctag ccactaaatt catcatattt gattcctttt
      121 ttaagataat gtagcatctt tttatccaat aacgcaattg tttttaagga taaatttctt
      181 aagatggtac atttcaaaat ggcatttgac atatgaaaca aaaaagaaag aaaagagcat
      241 aagggaaaac tgatagcacc ttcctacagg aaggtggcta agttggctgt gccaactggt
      301 tttctttcaa aatcatttca taatttttga tatcataaaa aaatccattt tggacctatt
      361 ttcggtcata atttagtacc tacttttggt catagtttcg tccgtagttt tggtcatagt
      421 gactaaggct tttctgttca caaaattggt ttaaatttgc tttctttaac acttcattta
      481 tatcatagca attataatgt ttacacaaca tcattcctaa ttttaacggt tgttcctctt
      541 tatatttttt aagttctaca tttttttgcg tttaattact tttctctttt ctttatattc
      601 cgctccaact ttctctttaa tagtgataat agaattacat atttttgtta aacttcctta
      661 aaaaaattgg atctcgatta ctcaaatttg cttgcaggct tttctaattg ctttgttttt
      721 tcttcctaat tatttgattt tatttccatt ttcttctagt gaaaatttat tatttttaat
      781 aatcgtacgc gtagaactga tttcattttt tgtatctgtt tgattatcta acgtttga
//
LOCUS       DAZBSA010000260          824 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000260 DAZBSA010000000
VERSION     DAZBSA010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.268"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(357..470)
                     /locus_tag="ACHGMT_002957"
                     /pseudo
     CDS             complement(357..470)
                     /locus_tag="ACHGMT_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          295 a          129 c          131 g          269 t
ORIGIN      
        1 attgaaacat ccatctggaa tttgtctttt tcgttgcttt gctcataaaa atctgcatat
       61 tcatcactaa tattttttct aataaattct tccatcataa agatcacctc gaaaagagta
      121 tacgaacaaa cgttcttttt gtaaaggtgg aatttattat actaaataaa taggtgaaca
      181 agtacttgtg ccaaattatg tgccaaaaaa aatcgaattt aataaaacac aaacaaaaag
      241 aaatcctatt atgataacat ttcttataat aacaaacaca acagaagacg tgtaatagtt
      301 agtcaggaac gtacaaataa cccctgtatc ctttgcggta cagggggtta ttttgtataa
      361 gtagacattg tagtttctaa actgctatgt cctaaacgtt tcgatacgct aagtatattt
      421 actccttgat aaagtaaaat agatgcatgc gtatgtctta gtccatcaat aaacagtcga
      481 agagaaaata gggaaaaatt gattactaaa gaaaaactta ctaggtcgac ggtgtagttg
      541 attttcatac ttcaatagat tcgaattaaa cgatttcgat tggcttatac cagtcctatc
      601 caacgaaaga tatatcgttc ttaactctag cggtataact tgaagcttta cgtaaagcca
      661 atgggaatga actggagatg actttttatt atttttagta atagctgatg ggttaatact
      721 tacaactttc cttttcaaag taccatataa aaaaacggtg gggttgttta gcgaaataaa
      781 ggatctaagc caatagctta gatcctttat tctatgcttt atat
//
LOCUS       DAZBSA010000261          805 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000261 DAZBSA010000000
VERSION     DAZBSA010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.269"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>805
                     /locus_tag="ACHGMT_002958"
     CDS             <1..>805
                     /locus_tag="ACHGMT_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HFN6836615.1"
                     /translation="EILINKGDLDDLFRRHLELAINALLQAELTVFLDYEKYDRAGFN
                     SGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLETTIIQLVQKG
                     ITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVIFMDATHIPL
                     KRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRGLEEVLLVVT
                     DGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV"
BASE COUNT          274 a          117 c          149 g          265 t
ORIGIN      
        1 tggaaatact aatcaataaa ggtgatttgg atgatttgtt tcgtcgtcac ctagaattgg
       61 ctattaacgc tttattacaa gctgaattaa cggtatttct tgactatgaa aagtatgata
      121 gagctggatt taattcaggt aattcccgta atgggaatta ttcgcgctct tttaaaacag
      181 agtacggtga attgaattta gtaataccta gagataggaa tggagacttt tctcaacaaa
      241 ctttacctgc ttataaaaga accaatgact ctttagaaac cactattatt cagctagttc
      301 aaaaaggaat tactatgtcc gaaatctctg atctaatcga aaaaatgtat ggtcattatt
      361 acaaaccaca aactatttca aatatgagta aaattgtgtc tgaagatgtt tcggcattta
      421 aagaaagaac tttagaagct aaatactcag tcatttttat ggatgctact catattccct
      481 taaaaagaca aaccgtatca aaagaagctg tctatatagt aattggtatc aggttggatg
      541 gaactaaaga ggttctagga tttactatag ctccaactga atctgcttat gtttggaaag
      601 agattcttca agatctaaaa gaccgtggtt tagaagaggt tttgttagtc gtaacagatg
      661 gtttaagtgg tattgacgat agtattcata gtgtttatcc aaatgctcaa tttcaacaat
      721 gttgtgtcca tatatctaga aatatcgctc ataaggttcg tgttagtgat cgacaagaaa
      781 tctgtagcga tttcaaatta gttta
//
LOCUS       DAZBSA010000262          785 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000262 DAZBSA010000000
VERSION     DAZBSA010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.270"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..192
                     /locus_tag="ACHGMT_002959"
     CDS             <1..192
                     /locus_tag="ACHGMT_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836616.1"
                     /translation="KEITKFSLEKVPFWGEFCLKIKCNWKRMYFYIDGDDALTFGKTT
                     FSSFNFQFLLKNNFYGLLK"
     gene            289..630
                     /locus_tag="ACHGMT_002960"
     CDS             289..630
                     /locus_tag="ACHGMT_002960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836617.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIGSGISSVRLKEKEYTQKIIHIPKPEIKRFEIKDY
                     SALGLFSIGYW"
BASE COUNT          268 a           82 c          133 g          302 t
ORIGIN      
        1 aaagagatta ccaagttttc actagaaaaa gttccttttt ggggagaatt ttgtttaaaa
       61 attaaatgta actggaaaag aatgtatttc tatattgatg gtgatgatgc tcttacattc
      121 ggtaaaacta ctttttctag ttttaatttt cagtttctat taaaaaataa tttttatggt
      181 ttattgaaat aaggttgcga aggaagatgt agagttattt gcactgttgt gttggtatat
      241 ctcttaatgc tgtgtctttt tggggagatt aattagtgga gtgattatat gagtatagat
      301 gattgtttag aagaacaaat aagattattt ttaaagtttt taaaagttaa agttcaaaat
      361 aactacttca catgttatag ttatgcacac cctgttttaa atagtcttcc ttttaattct
      421 ttagtgcaag gattgattac aaatattgtt ttattagtct gtacaccaaa tgaaatattt
      481 gtaaaaaaga taggcagtgg tatttcttca gtaagattaa aagagaaaga atatactcaa
      541 aagattattc atattcctaa gcctgaaatt aagcgttttg agataaaaga ctattctgct
      601 ttagggctgt tctcaattgg ttactggtga taaaaaataa gaatactaaa actatacgta
      661 gaatataagt aatcggcatc aatcctaatt atgtgattga atgccgattt ttgttacgcg
      721 ctatttttaa ttatctgtaa gaagaataat tttattttac tcatattcgt tttcactttg
      781 aagaa
//
LOCUS       DAZBSA010000263          752 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000263 DAZBSA010000000
VERSION     DAZBSA010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.271"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(22..94)
                     /locus_tag="ACHGMT_002961"
     tRNA            complement(22..94)
                     /locus_tag="ACHGMT_002961"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(59..61),aa:His,seq:gtg)
     gene            complement(97..170)
                     /locus_tag="ACHGMT_002962"
     tRNA            complement(97..170)
                     /locus_tag="ACHGMT_002962"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(136..138),aa:Trp,seq:cca)
     gene            complement(177..257)
                     /locus_tag="ACHGMT_002963"
     tRNA            complement(177..257)
                     /locus_tag="ACHGMT_002963"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(221..223),aa:Tyr,seq:gta)
     gene            complement(259..334)
                     /locus_tag="ACHGMT_002964"
     tRNA            complement(259..334)
                     /locus_tag="ACHGMT_002964"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(299..301),aa:Phe,seq:gaa)
     gene            complement(370..442)
                     /locus_tag="ACHGMT_002965"
     tRNA            complement(370..442)
                     /locus_tag="ACHGMT_002965"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(407..409),aa:Asp,seq:gtc)
     gene            complement(452..524)
                     /locus_tag="ACHGMT_002966"
     tRNA            complement(452..524)
                     /locus_tag="ACHGMT_002966"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(489..491),aa:Val,seq:tac)
     gene            complement(540..611)
                     /locus_tag="ACHGMT_002967"
     tRNA            complement(540..611)
                     /locus_tag="ACHGMT_002967"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(576..578),aa:Glu,seq:ttc)
     gene            complement(626..715)
                     /locus_tag="ACHGMT_002968"
     tRNA            complement(626..715)
                     /locus_tag="ACHGMT_002968"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(679..681),aa:Ser,seq:gga)
BASE COUNT          209 a          206 c          173 g          164 t
ORIGIN      
        1 ctatacccca agaataaaaa gggcgaatga tgggaatcga acccacgaat gccggagcca
       61 caatccggtg cgttaaccac ttcgccacat tcgccatggc aggggcagca ggaattgaac
      121 ccacactaac ggttttggag accgctgttc tacctttaaa ctatgcccct ataaaatgga
      181 ggagagtgga ttcgaaccac tgaaccctaa ggaacggatt tacagtccgt cgcgtttagc
      241 cacttcgcta ctcctccatg gtggcgcggg acagaatcga actgccgaca cacggagctt
      301 caatccgttg ctctaccaac tgagctaccg cgccatattt taaaattatt aattgaataa
      361 caagaacgac ggttccgacg ggaatcgaac ccgcgatctc ctgcgtgaca ggcaggcatg
      421 ttaaccccta caccacggaa ccagttcatt atggaggtta acgggatcga accgctgacc
      481 ctctgcttgt aaggcagatg ctctcccagc tgagctaaac ctccaacatt caattaaaat
      541 gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag ggcggtgtct taaccgcttg
      601 accaacgggc caggtaaatt tgctacggag agtaagggat tcgaaccctt gagacagttt
      661 ggcaccatct acatgatttc caatcatgct ccttcggcct ctcggacaac tctccagagt
      721 gaaagaagct caatactctt tctcaaaatg ag
//
LOCUS       DAZBSA010000264          734 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000264 DAZBSA010000000
VERSION     DAZBSA010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.272"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>734
                     /locus_tag="ACHGMT_002969"
     CDS             <1..>734
                     /locus_tag="ACHGMT_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HFN6836618.1"
                     /translation="KNNLKKKMLIPFDSEVKGLLNKLTISNIDSTREKIIKIFDKINN
                     LFKNDNAELRKGLLEFKLQELELHYSYLVKITEEKEQQKAIKEQMIEEEKVRREIDRE
                     KKRIDKEQRQFNSEISKLIAYMQKANADVEKELYANKIQELEEKLKELEVIRENVLQR
                     ELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEPMDRIKELSSASVPFEFDVHAMIFSE
                     DAPSLETKLHNHFRKQEVNKINQRKE"
BASE COUNT          326 a           77 c          126 g          205 t
ORIGIN      
        1 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
       61 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      121 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      181 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      241 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      301 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      361 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      421 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      481 gagaattaaa tactagagcc ggttatgtgt atgtaattag taatattggg tcttttggtg
      541 aagacatata taaaattggt atgactagaa gattagaacc tatggataga ataaaagaat
      601 tgagtagtgc atcggttcca tttgaatttg acgttcatgc gatgattttt tcagaggacg
      661 ctccgtcttt ggaaactaaa ttgcataatc attttagaaa acaagaagtt aataaaatta
      721 atcaaagaaa agaa
//
LOCUS       DAZBSA010000265          707 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000265 DAZBSA010000000
VERSION     DAZBSA010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.273"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..>707
                     /locus_tag="ACHGMT_002970"
     CDS             64..>707
                     /locus_tag="ACHGMT_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN6836619.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSLDKDYELLNPIIKNIFTKYYSKDFEIYLNEILANNINPLIEEYANQRH
                     SISKELFFKRANSKLQDKIRNGDFKYGEKERMIDSLKQLLDEMDLAYKDISKYSS"
BASE COUNT          292 a          107 c          104 g          204 t
ORIGIN      
        1 ttttaaatac ttttatttta taaatatgat atactatctt taaaaataaa aaggagaaaa
       61 aagatgaagc caaatccttc agaagcagga aaacgaatca aacaattacg cctttcatgt
      121 ggttttacta tggaagaact tggaaggaaa atagataatt caccacgtgc gaccatatcc
      181 aactgggaac gtgggaccaa tttaccaaat ccacaaaagc tgaaattatt atcaactatc
      241 actaattcaa caattgactg gattaaatgg ggaactttag aagaatatat aacttcatac
      301 cttatcgata tagggtatga gctatatatt aaggacttcc cagaaatacc tcacaaagtc
      361 tttaaagaca ttcaagaaag atatagtgat acgttttcgc tagataaaga ctatgagtta
      421 ttgaatccta taataaaaaa tatatttaca aaatattact caaaagattt tgaaatttat
      481 ctaaatgaga tacttgctaa taatatcaat cctctgattg aagagtacgc taaccaaaga
      541 cactctatct ctaaagagct atttttcaaa agagccaaca gtaaacttca agacaagata
      601 agaaatggtg atttcaaata cggtgaaaaa gaaagaatga ttgattcttt aaaacagcta
      661 ttagacgaaa tggacttagc ctataaagat atttcaaagt attcatc
//
LOCUS       DAZBSA010000266          696 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000266 DAZBSA010000000
VERSION     DAZBSA010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.274"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..629)
                     /locus_tag="ACHGMT_002971"
     CDS             complement(54..629)
                     /locus_tag="ACHGMT_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFN6836620.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          230 a          151 c          124 g          191 t
ORIGIN      
        1 aatgaaaaaa cagagccaga ttagcattat tttgctaatc cagctctgtt tattcagcct
       61 atttttcttt ttgaatatat caagcgttgg ggcaacatag tgatgactaa tgaaacaacg
      121 acgatagaca acaagcgatc aaggtagtct gtacccgcct gtaccaagat cacacttgtt
      181 gtctgactta aacctaaccc atgtaatagc tgaacgatca tactggatcc tgaggaggtg
      241 atcccgccaa ataactgaac ggtaatgatc gaagaaacga tcgttccagg taccgacaaa
      301 atcaaagcga gtaataacag tttcaagctg tttttatctt tgccgagata tcttggcaaa
      361 atcaatccag caagcaagcc tgtgagaatt tggattggcg aataatacag agaaaaaata
      421 tctgtagtca ctccacttaa aataccgctg gacaatcctg tcagcattcc aggtacaggc
      481 ccaagcaatg caccagcaaa aatcgttccg atagaatcga gatagattgg caaacgaagc
      541 cataaagcaa tcgttcctcc aatataattg atcccaatac acaaagccat cactgtaagt
      601 acgcgagttg ccattttgtt tttattcatt ttgttagccc atctttaacc tttccaaatc
      661 tttcataatc gtttcttctt gtgcatttaa aagaac
//
LOCUS       DAZBSA010000267          683 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000267 DAZBSA010000000
VERSION     DAZBSA010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.275"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..660
                     /locus_tag="ACHGMT_002972"
     CDS             <1..660
                     /locus_tag="ACHGMT_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFN6836621.1"
                     /translation="VSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSR
                     GVQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKA
                     VYQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTFYDFPPSIRRTIYSTNLI
                     ESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFT
                     E"
BASE COUNT          251 a          124 c          118 g          190 t
ORIGIN      
        1 gtatccaaag aagcgattta tattgccatt ggtatacgag aagacggcac taaagaagta
       61 ctgagttatg cgattgctcc aactgaatca acatacgttt ggaatgagct gctacaggat
      121 attaactcca gaggagttca agaagtcttg ctttttatta cggacggctt aaaaggcatg
      181 aaagatacta tccatcaaat ttatcctaaa gcaaaatatc agcattgttg tatccatgta
      241 tctcgtaaca tcgctcataa agtacgtgtc aaagaccgaa aagaaatctg tgatgacttt
      301 aaggctgttt atcaagctaa ctcaaaagaa gaagcgaata ccttcttatc cggcatgatt
      361 gagaaatgga agaaaaacta tcctaaagtg acgcagtcac tcatagaaaa ccaagactta
      421 ttaacttttt atgattttcc acctagcatt cgtagaacca tttactcaac caatctaatc
      481 gagtctttca ataagcaaat taaaagatac agccgtagaa aagagcagtt tcaaaatgaa
      541 gaatcactag aacgctttct agtcagcatt tttgatacat acaatcaaaa atttctaaac
      601 agaagccata aaggttttca acaggtaacc gatacattag tttcaatgtt tactgagtaa
      661 ctaattattt tgcaggagga caa
//
LOCUS       DAZBSA010000268          682 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000268 DAZBSA010000000
VERSION     DAZBSA010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.276"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..613)
                     /locus_tag="ACHGMT_002973"
     CDS             complement(<1..613)
                     /locus_tag="ACHGMT_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFN6836622.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
BASE COUNT          195 a          133 c          133 g          221 t
ORIGIN      
        1 gcaggaagta ttgagttagt ttgtaaagtc tgcgatcttc aaggatcgta aatagctgat
       61 gggttgtccc atcaacacaa ataaaactca tggaaccaga aacagacttc attgatttga
      121 actcatccat acacaacacc gtcggaagga agttcatacg tgactggtaa tgcttcgttg
      181 cttcacgcaa gatccgtgtc accgtcccat ccgagacgaa gtacttctta gcgattagtt
      241 tacgtgattg attttcgtac agttccatga gaattgcctg cttcagttcc tttgttagtt
      301 gatgatgttc atcaactaag tccgttttcg cagaaaaggt ccttccacaa tcatggcaga
      361 gaaaacgggt acgttttaat tctaaaatag ttaacttttc tttgactttc aatatctgag
      421 tgcgtgtcgt ataggaaccg ttgcggataa tacgaaccga tcgacagtgt tcacaatagg
      481 aaggagagtc atccaatttt cctttaataa actgcgcttg aacgccttta tattcttttt
      541 caatcagcca ttcttcatca aaaaataaat gtttatctgt taatccgagt aattttctag
      601 tatgattatc cattgagaga ctcctttggt aggatgacgt tttggtcgac taaattttac
      661 caaaagtgag tctctttttg ag
//
LOCUS       DAZBSA010000269          681 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000269 DAZBSA010000000
VERSION     DAZBSA010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.277"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..445)
                     /locus_tag="ACHGMT_002974"
     CDS             complement(<1..445)
                     /locus_tag="ACHGMT_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFN6836623.1"
                     /translation="MNKDITFPVMDVRLVPITKIQANDYNPNRVASPEMNLLELSIIE
                     DGFTQPLVCYYKEKEEKYILVDVLHRYRVAKERLNLKVVPVTVIDKPIEERMASTIRH
                     NRARGTHEIRGMEIVIQRLVKNGWSDKRISKELGMDIEEIFRFKQV"
BASE COUNT          202 a          136 c          104 g          239 t
ORIGIN      
        1 acacttgctt aaagcgaaaa atttcttcaa tgtccatgcc taattctttg gatattctct
       61 tgtctgacca tccatttttt actaatcttt ggatgacaat ttccattcct cttatttcat
      121 gggtccctct tgcacgattg tgtcgaattg ttgaagccat acgttcttct atgggtttat
      181 caataactgt taccggtaca acttttaagt tgagtctttc cttagcaaca cgatacctat
      241 gaagcacatc aaccaagata tacttctctt ctttttcctt gtaataacaa actaatggtt
      301 gagtaaagcc atcttcaatg atagaaagct ccaataagtt catttccgga gacgctactc
      361 gattgggatt ataatcgttc gcctgtattt tggtaattgg aacaagccga acgtccatca
      421 cagggaaagt tatgtcttta ttcatacgct tgtataccct actttcttaa aaagtagggt
      481 atactcaaga atccgattaa ttatcttctc aatttggata atatatctca tcagtcttct
      541 tgtcacttta tcaacatgat tttgctgcaa aatgggatac cgacagtact ggactggtaa
      601 tcaggataaa agcaaaaata attaatttgt atcctaaaaa atccataaaa attctctcac
      661 attttcgggt attaattcga g
//
LOCUS       DAZBSA010000270          654 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000270 DAZBSA010000000
VERSION     DAZBSA010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.278"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..652
                     /locus_tag="ACHGMT_002975"
     CDS             <1..652
                     /locus_tag="ACHGMT_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFN6836624.1"
                     /translation="LALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIA
                     KNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLI
                     EEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIE
                     NFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          281 a           83 c          107 g          183 t
ORIGIN      
        1 tttagcgtta aagatgaaag gatatacaat caatcataaa acagttagaa aattaatgtc
       61 ccaaatggga cttacctgtc aaatccgaat aaagcgatat aaatcttata aaggaacagt
      121 aggaaaaatt gccaagaatg tgttaaaacg aaatttttca gtagatacac ccaataaaaa
      181 atgggtgaca gacgtcactg aattcaagat aaaaggaaga aaaatctatc tatctcctat
      241 acttgattta tttaacggag aaataattag ttatagtata tcaactagtc caacatataa
      301 actgattgaa gaaatgcttc aacaagcaat taaaaaaaag ggaactgagg gttcactgat
      361 tctacattca gatcaaggat ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga
      421 gaataacatt attcaaagca tgtctagaaa aggaaattgc ttagataatt ctgtaataga
      481 aaattttttt ggtgtgctta aatcagagtt tttttatcga gaaaaattcc gatcaattga
      541 gatatttcaa agtaaattaa atgagtatat taggtggtat aataacaaaa ggataaaact
      601 aaagttaaat ggactatctc cagtagaata tcggaaacag tccattaaat agtt
//
LOCUS       DAZBSA010000271          626 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000271 DAZBSA010000000
VERSION     DAZBSA010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.279"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..548)
                     /locus_tag="ACHGMT_002976"
     CDS             complement(<1..548)
                     /locus_tag="ACHGMT_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /protein_id="HFN6836625.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGA"
BASE COUNT          246 a          104 c           68 g          208 t
ORIGIN      
        1 gctccaacat ttggacttgt atagacacca aataaccgat gttcaataaa accttgtctt
       61 gcaagtaatc ctggcgttcc tgttgggaca tgataagaaa tcaaaaaaat aaattgatag
      121 atagatatta gcgcagctaa aaaaattatg ataatgaaaa cattattaaa atacaccatt
      181 tttttctttt tttcctcttc actttgtgaa aagtctgcag gaaaaattac tatcaaagta
      241 atcagcaaat atccaaaatt ataaatagat ggaattaaaa aattttctct gttaatgatt
      301 atagttaaga taaaactaat cattgctcca atcagtagcc aagaaaaacg cattttaaaa
      361 aaatttttct ttataaaaac ctcatacaaa actataattc caccccaagc aaaagctatt
      421 ttcatcggga tattagttac attaaaagta ctaggcataa aataaagcag acaaatgatt
      481 aaatttatta ctcgaaacgc atctcgactt aataaactct tctctaaaac aggtactatt
      541 ctattcatct atatttccaa ctttcaaaat ttctttatca attctattta atagtttatc
      601 agagtaatcc gatttaactt ttctta
//
LOCUS       DAZBSA010000272          625 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000272 DAZBSA010000000
VERSION     DAZBSA010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.280"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..625
                     /locus_tag="ACHGMT_002977"
                     /pseudo
     CDS             1..625
                     /locus_tag="ACHGMT_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          247 a          105 c          118 g          155 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcctt atcaaaacga ggcattcaga
      361 tcagccgacg aaaaattagt agaatcatga aaaatagagg attaaaatcg agctatactg
      421 ttgcttattt taaagtacat cattctactt gcaatgaagc caaaacgaca aacgtattga
      481 atcgtaaatt cttaagagac aacccattag aagcgatcgt aacagacttg acttatgtac
      541 gagtcgggaa aaaatggaat tatgtctgtt tcattttgga tctgttcaat cgagaaattc
      601 tcggctattc ttgtggagaa cataa
//
LOCUS       DAZBSA010000273          611 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000273 DAZBSA010000000
VERSION     DAZBSA010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.281"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..418
                     /locus_tag="ACHGMT_002978"
     CDS             <1..418
                     /locus_tag="ACHGMT_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN6836626.1"
                     /translation="PKVEYIYEFEGGKGITNQYRPNKFETFKEFYSLANTVTDDINKD
                     GMAALLLKGTTSVIFSIYSTSARTSKEKKDEAKQILSDPVIREAKQIAKTDSSAKKIT
                     KLLYNMPAPLFLSAVKVGSFVEVKMPGLMAALKRVY"
     gene            437..>611
                     /locus_tag="ACHGMT_002979"
     CDS             437..>611
                     /locus_tag="ACHGMT_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFN6836627.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGR"
BASE COUNT          219 a           85 c          127 g          180 t
ORIGIN      
        1 cccaaaagta gaatatattt atgaatttga aggtgggaag gggatcacta atcaataccg
       61 acctaacaag ttcgaaacat ttaaagaatt ctattcatta gcaaatactg taacagacga
      121 tattaataaa gacggtatgg cggcgcttct tttaaaaggg acaacttcag ttattttttc
      181 tatttatagt acctctgcaa gaacttcaaa agagaagaaa gacgaagcaa agcagatatt
      241 gagtgatcca gtgatccgag aagccaagca aattgctaag acggattctt cagcaaaaaa
      301 aataacaaag ttgctttaca atatgccagc tccactattt ttatctgctg taaaagttgg
      361 aagcttcgtt gaagtgaaaa tgccagggtt aatggctgct ttgaagcgag tatattagtt
      421 aatggaggaa aatataatga aaagaatttt gcattttcag ggacgaatgg gtttaggtgg
      481 agcagagtct ttcatgatga atttatatcg taaaattgat cgtacaaact atcaatttga
      541 ttttttgatt tatgaagatt atgcagatgt tcaagactat cattcagaga ttgaaagatt
      601 aggaggccgt a
//
LOCUS       DAZBSA010000274          561 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000274 DAZBSA010000000
VERSION     DAZBSA010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.282"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..>561
                     /locus_tag="ACHGMT_002980"
     CDS             73..>561
                     /locus_tag="ACHGMT_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFN6836628.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMD"
BASE COUNT          210 a          103 c           92 g          156 t
ORIGIN      
        1 catacaaaaa aggaagtccc ttctgtagaa taaagttaac gacaaccaat tcacagaaaa
       61 gaggacttcc ctatgaatga ttttactaca gaaattgtgc aaactctagt cactaaaggc
      121 gatttaaatg aattattccg ttcgcactta gaaaaagcga taaacacact cctacggact
      181 gaattaacgg cttttttaga ttacgaaaaa tatgatcgca ctggttttaa ttcaggtaat
      241 tcgagaaacg gttcttactt tcgatcaatc aaaaccgaat atggtgaatt aacattggaa
      301 atacctagag atcgtaatgg tgagtttaaa caacaaactt taccagccta caaaagaaca
      361 aacgatacat tggaaaccac tattatccat ttattcgaaa aaggtgttac gatgtctgaa
      421 attgctgatt tgatcgaaaa aatgtacggt catcactata ctccacaaac catgtccaac
      481 atgactaaag ttctgactga agaagtaaat gcctttaaat ccagagcctt aaatgataag
      541 tatgtcgcta tttttatgga c
//
LOCUS       DAZBSA010000275          548 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000275 DAZBSA010000000
VERSION     DAZBSA010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.283"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..179
                     /locus_tag="ACHGMT_002981"
     CDS             <1..179
                     /locus_tag="ACHGMT_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFN6836629.1"
                     /translation="IKLAKILPLLKEPEQIFLIEKFIFDKTDKEIGKVLGITRQGATN
                     LKHRLYKKIQKMLV"
     gene            273..464
                     /locus_tag="ACHGMT_002982"
     CDS             273..464
                     /locus_tag="ACHGMT_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN6836630.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT          211 a           75 c           88 g          174 t
ORIGIN      
        1 atataaaatt agcaaaaatc cttcctttgt tgaaagaacc tgaacaaatt tttcttatcg
       61 aaaaatttat ctttgataaa actgataaag aaattggaaa ggttctgggg attacaagac
      121 agggtgcgac aaatttaaaa catcgacttt acaaaaaaat tcaaaaaatg ctggtttaat
      181 atctgcataa taactaattt tgtatgcttc tttgctcgtt ctcctccttc ccaaagaaat
      241 agattataag tccatgggaa gaaggtagat ttatgaattc tttaaaagaa gatttcattt
      301 tagcaaaggc tggaaatgaa gaagctgttg aggcaattct caaaagattt agctctttga
      361 ttcataagca atcctggaga actggaaaat atgatcaaga ttgttatcaa gaatgtatgc
      421 ttgcaatata tctagctatc tcgaaattcg aaattaaaga ataactcaac taagggagtc
      481 tgtgacataa gtaaaaagat agtttcaaat ttcgaacaat aaatttgaaa ctatcttctt
      541 tataatta
//
LOCUS       DAZBSA010000276          545 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000276 DAZBSA010000000
VERSION     DAZBSA010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.284"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(215..361)
                     /locus_tag="ACHGMT_002983"
                     /pseudo
     CDS             complement(215..361)
                     /locus_tag="ACHGMT_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          162 a           95 c           99 g          189 t
ORIGIN      
        1 atagttgaga gacatctata tggtgtctct ttttttatta atatgcaaaa aagtctagtt
       61 ttttcaataa tatttatgta ataataaatt agccaccaca acaaagaatg aaatccatta
      121 ttatctagtc tagtccattc tttttgtttg cagtatttgt gatggctttc cgtaccctta
      181 gctcagttgg tcagagcaga cggctcataa ccgtccggtc gtacttctcg taatccagga
      241 aggccgtcag ctccgtccgc agtagcgtgt ttacggccag ctccagatgg gagcggaata
      301 actcgttcaa atcacctttg gtggctagag tctgcaggag ttctgtaata aaatcgttca
      361 tagggaagtc ctcttttctg tgaatgggtg tggtaacttt attctacaga agggacttcc
      421 ttttttctat ttacacaaaa tattttatac tctcaaatgt ttgtatgcag ttacatctga
      481 ctttattcga attaaggaaa agatttataa aaataaaata aaaaaatata tatttaaatt
      541 gactt
//
LOCUS       DAZBSA010000277          544 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000277 DAZBSA010000000
VERSION     DAZBSA010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.285"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>544
                     /locus_tag="ACHGMT_002984"
     CDS             <1..>544
                     /locus_tag="ACHGMT_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HFN6836631.1"
                     /translation="RFHIIKHMNQAFNELRIREMNELRKVGQKSQAEKLKKNWRFLLK
                     NRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHELVEAFRDKD
                     PDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNGKIEAKNTHIKTMKR
                     VSYGFKSFENMRIRIFLINQL"
BASE COUNT          192 a          106 c          100 g          146 t
ORIGIN      
        1 tcggtttcat attatcaaac acatgaatca agcctttaat gagttgcgta tccgtgaaat
       61 gaatgaactt cgtaaagtag gacagaaaag tcaggcagaa aaactgaaaa agaactggcg
      121 ctttttgcta aaaaatcgtg caaacatcaa ccattatgaa tacaaaacat ggaaaagttt
      181 tcgagcacca aaatacccat ttcttacgga agcgatgatg attgatcgat tgcttggctt
      241 ttctacgtcc ctgaaggagg cgtatcccta ttttcatgaa ttagttgaag cctttcgaga
      301 caaagaccct gacttatttt tctccttatt ggcagaactt cccgaaacgt tagatgacgg
      361 ctttcgggaa aagctccaaa accttctgac ctatgaagaa ggcatcacca acgcgatgat
      421 ctatccttac accaatggaa aaatagaagc gaagaatacc catataaaga caatgaaacg
      481 agtatcctac ggatttaaat catttgagaa catgagaatt agaatctttt tgatcaatca
      541 atta
//
LOCUS       DAZBSA010000278          534 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000278 DAZBSA010000000
VERSION     DAZBSA010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.286"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>534
                     /locus_tag="ACHGMT_002985"
     CDS             <1..>534
                     /locus_tag="ACHGMT_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFN6836632.1"
                     /translation="DGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDL
                     KPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNT
                     SAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIR
                     GLANGRMQTYEQNTELG"
BASE COUNT          163 a           80 c          137 g          154 t
ORIGIN      
        1 tgatggattt aaggcctgta ctgatccaag acaagcagca tatgcttttg aacgaaacta
       61 tgaacgtccg gcagcgacac atcctgaacg tcaagattat gcagttaact ggtataacaa
      121 atttaaagat ttaaaaccag gaggagctac tggagaagca ggactaaaac atttagagtc
      181 tttgattgga caacgaattg gcaatggtca gtgttatggc ttgtctgcag aatattcagg
      241 atatctgggt ggttgtggca tgggtgctgg aacaaaatat ggtttaactc atgtgatagg
      301 aaatacttct gcagcatctg atattggtat tgcctatgat tggtctgctg ttggttggaa
      361 agtgattcaa aaccctagat atgatcaatt agtagttggt gcaattatta attgggcaag
      421 aggtggacaa gtgggttcat ggtttgcaga tggaacttat ggacatactg gcgttatccg
      481 aggcttagct aatggtcgta tgcaaactta tgaacaaaac acagagttag gtat
//
LOCUS       DAZBSA010000279          514 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000279 DAZBSA010000000
VERSION     DAZBSA010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.287"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACHGMT_002986"
     CDS             complement(<1..132)
                     /locus_tag="ACHGMT_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFN6836633.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DAZBSA010000280          512 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000280 DAZBSA010000000
VERSION     DAZBSA010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.288"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..287)
                     /locus_tag="ACHGMT_002987"
     CDS             complement(<1..287)
                     /locus_tag="ACHGMT_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836634.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKWLPMIDQLVSNNKEAKNLCIFVLLLHGRVINLVNR"
BASE COUNT          178 a           89 c           54 g          191 t
ORIGIN      
        1 tttcgattaa ctaagttaat aactcttcca tgtaataata agacaaaaat acataaattt
       61 ttagcttcct tattattaga tactaattga tcaatcatag ggagccattt tttgattttc
      121 actaattgat tgctcataaa tctttcactg gacttttgtt caatcttaaa ttgcaaaact
      181 tctaactcat aattcccatc ttctgattta tttttgggaa caactatcaa acttttataa
      241 agtttatcca catcaaaatt tatctttttt gcataatttg tagccattaa tacatctcct
      301 tattcataat aaatttcata tgacgctcct ctcaaaattt accggtagac tagaaaaaag
      361 aataaaagat acgtcaattc acactttatt cttaaaaaaa aactggtctc tctaccggta
      421 atatcgattg agtccgcctt tatcgacttt atcctttttt tatattaaat tttttcaccg
      481 gtagacaggg agagaaagaa taaactaaaa ag
//
LOCUS       DAZBSA010000281          476 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000281 DAZBSA010000000
VERSION     DAZBSA010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.289"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(108..>476)
                     /locus_tag="ACHGMT_002988"
     CDS             complement(108..>476)
                     /locus_tag="ACHGMT_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN6836635.1"
                     /translation="KQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFD
                     NAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQ
                     RLAQRILDNELGCANASEAV"
BASE COUNT          137 a          103 c           92 g          144 t
ORIGIN      
        1 gacaaatttt ataaggtgca gaacttcttt ccgtatgcta ttccgattgt ccttgacaat
       61 gagcctcctc ggatgtgacg atcttcggca ggagctaaca gttaaagtta gactgcctcg
      121 ctagcgttag cacatccgag ctcattatca aggattcgct gcgccaatct ctggttacgg
      181 taaccaaccg gtgtctcgta gccaagtgta ccgtgcaacc gaaggtggtt ccaccaattg
      241 acatagtcaa ataactccaa atccaattgt tgtaaggttt caaatgtgta ttgatagaca
      301 aattctactt tcaacgactt ataagttgat tcagctacgg cattatcaaa aggacagcct
      361 ttatgactca atgatcgatt gatgtcaaaa gttgttaata attcatcaat agcttggtta
      421 tcaaactctt ttccacgatc agtatgaaaa atctcaacct ctgtcagagg ttgttt
//
LOCUS       DAZBSA010000282          457 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000282 DAZBSA010000000
VERSION     DAZBSA010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.290"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          149 a           88 c           52 g          168 t
ORIGIN      
        1 acaaaaaata aaaaaattat tacgttatta aaaaaacaga tgaaagatca ggttttatta
       61 gatctcattc tttcatcact tgaagggatt taatgaacta ctttttcctc tttgtcgttt
      121 ttatatattc gtattccttc aaacagtagg atagcctctt tcaatgcttg atatttgaac
      181 tgtccgttac ttaaaaattt agatgtgccg ccctcttcct agaacatatt cttttttact
      241 ttttcattaa ttgacagacc aatcgaacga aactgtaaat ttcgatagga taatgattgg
      301 tcaaactcat tcattttcaa gacatctcga ttcatattca agataggaat ctttttatca
      361 acatcacttt attcgtattt tcctctacca taaaagcttc acgctccaca aaattgtttt
      421 atacactact attcagccca ttcaacgacc aatgaat
//
LOCUS       DAZBSA010000283          451 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000283 DAZBSA010000000
VERSION     DAZBSA010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.291"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>451
                     /locus_tag="ACHGMT_002989"
     CDS             <1..>451
                     /locus_tag="ACHGMT_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN6836636.1"
                     /translation="FRKGITMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKVR
                     TLNDKYVAIFMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNE
                     LLQDIYSRGVHDVLLFITDGLKGMKDTIHQIYPKAKYQHCCVHVSRN"
BASE COUNT          147 a           89 c           87 g          128 t
ORIGIN      
        1 tcttccgaaa aggtattacc atgtcggaaa tcgcagactt gattgagaaa atgtatgggc
       61 atcactacac gccccaaacc atgtccaaca tgactaaagt gctgactgaa gaagtaaatg
      121 catttaaagt tagaactcta aatgataagt atgtcgctat tttcatggat gctacttata
      181 ttccattaaa acggcaaacc gtctccaaag aagcaatcta tattgccatt ggtatacgag
      241 aagacggcac taaagaagta ctaagttatg cgattgctcc gactgaatcg acctacgttt
      301 ggaatgagtt gttacaggac atttactcca gaggggttca tgatgtctta ctgtttatta
      361 ccgatggctt aaaaggcatg aaagatacga ttcaccagat ttatcctaaa gctaaatacc
      421 aacattgctg tgttcacgtt tcccgtaata t
//
LOCUS       DAZBSA010000284          446 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000284 DAZBSA010000000
VERSION     DAZBSA010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.292"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            249..>446
                     /locus_tag="ACHGMT_002990"
     CDS             249..>446
                     /locus_tag="ACHGMT_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836637.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVE"
BASE COUNT          186 a           46 c           87 g          127 t
ORIGIN      
        1 gatgtaaaca agattttttt cagaagaaaa aacgataaaa gttgtgataa acgatttaaa
       61 aaataggaga aaatatatca agcgcatggt atgaaacgca catttaaaaa gattcgacta
      121 actaaaaaaa taagtttaca aagcattgtt gtgtattaca agtgatctat acactgaaaa
      181 atgatagaga aaatctgata cgtccgcaac aatggacaat tgaaaatgaa agtgaggaaa
      241 ttggaagaat ggctgatgaa aataaagttg ttccgcttga aacactagtg ataaataagg
      301 aagagtttga gcaacttttt cttgattttt ttgttcaata tgacaggatt tatgatttta
      361 gttcgtcact agatttattt gaattagagg cagaaataat agaaaacagt cgagaggaga
      421 atggtaaaat catcatttca gtagaa
//
LOCUS       DAZBSA010000285          443 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000285 DAZBSA010000000
VERSION     DAZBSA010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.293"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          143 a           81 c           75 g          144 t
ORIGIN      
        1 atctgttcta acattctcaa ctttgtcagt ttaccatagg aaaatgggaa aatctacgaa
       61 agctctcaac agaatcgtcc tacttaaata cgcttttctt taataataaa taagttggtt
      121 gtttttgtta tttgcgcttt cacttttcgg aataaataat gtgtatttat atggaataaa
      181 taaaaaaaga ggtcgtgact aaaaacaagt cacaacctct ggtaaacggt gttctcctga
      241 caactcctcg gtcacaagac acaatattca acaaacatga gaagctattt tttgaaattg
      301 ctctttgtga ctcagagtga aacggcaggc tcacgacctc tttcttaaac ggtgttcaaa
      361 agcttgcatc tgcggtaata agctcaacca aacgtaaaat atgaggaact atttttgttt
      421 ggctgttctt attacttgat gca
//
LOCUS       DAZBSA010000286          427 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000286 DAZBSA010000000
VERSION     DAZBSA010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.294"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..214
                     /locus_tag="ACHGMT_002991"
     CDS             <1..214
                     /locus_tag="ACHGMT_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN6836638.1"
                     /translation="LLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQSYLILIS
                     IIVLSVVFASSLMLIKKPKEILSKIS"
     gene            224..>427
                     /locus_tag="ACHGMT_002992"
     CDS             224..>427
                     /locus_tag="ACHGMT_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFN6836639.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNED"
BASE COUNT          171 a           43 c           72 g          141 t
ORIGIN      
        1 attactaaaa gtaattgtag atggattcat taactcagag gattcaatga tttccggtgg
       61 aagtttaata aataatagta gttttatgtc aaatataaca acacttggac aaagttattt
      121 aatattaata agtattattg ttttatcagt tgtatttgct tcttcattaa tgttaatcaa
      181 gaagcctaaa gaaatcttat caaaaataag ttaggagaaa ataatggata tattagaaat
      241 aaagaatgta gcatatagtt atgcaaattc taaagaaaaa gttttgtcag gagtaaatca
      301 aaaatttgaa cttgggaagt tttatgcgat agtaggaaag tcaggaacag gaaaatctac
      361 acttctttcc ttacttgctg gacttgataa acctcaaaca ggaaaaatat tgtttaagaa
      421 tgaagat
//
LOCUS       DAZBSA010000287          414 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000287 DAZBSA010000000
VERSION     DAZBSA010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.295"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(109..>414)
                     /locus_tag="ACHGMT_002993"
     CDS             complement(109..>414)
                     /locus_tag="ACHGMT_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN6836640.1"
                     /translation="TIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          116 a           96 c           82 g          120 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggt
//
LOCUS       DAZBSA010000288          375 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000288 DAZBSA010000000
VERSION     DAZBSA010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.296"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACHGMT_002994"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACHGMT_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFN6836641.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          122 a           56 c           61 g          136 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttgtatagac tgattgtaac atggaacaaa ataaaaaggc
      361 aatacgcctt agttg
//
LOCUS       DAZBSA010000289          371 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000289 DAZBSA010000000
VERSION     DAZBSA010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.297"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(40..339)
                     /locus_tag="ACHGMT_002995"
     CDS             complement(40..339)
                     /locus_tag="ACHGMT_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFN6836642.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT          125 a           71 c           50 g          125 t
ORIGIN      
        1 ataaaatccg ttccgggcta cctttttagg tagcctactt taatctttgt atctttctgg
       61 atcaacgaaa gtatacttta tatagtcata acgccgatga tcgcttcgag cgtctagcac
      121 atcagtcacg atatcaaaca aaaaatatac gtctttcttc attctagttt tcgcagcagg
      181 gattttaaag tagttcttat tagaatagta gagattaatt aataagctat cttcgattgc
      241 taaaaagaaa acttctgaat cccacacctt ataaaaatct ttgacaaatc tattcgaagg
      301 atcaaattta aaccataatt gcgtctcatt aaaaagcata accattactc cgatctattt
      361 ttaaacttag t
//
LOCUS       DAZBSA010000290          341 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000290 DAZBSA010000000
VERSION     DAZBSA010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.299"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>341
                     /locus_tag="ACHGMT_002996"
     CDS             <1..>341
                     /locus_tag="ACHGMT_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFN6836643.1"
                     /translation="EATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEP
                     DAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNR
                     SDADKYEPTVEG"
BASE COUNT          141 a           50 c           76 g           74 t
ORIGIN      
        1 gaagcaactc aaccaattac agcagatgat acagaatttg tatcaagtgc aacaaaactg
       61 gatgataaat ctgacgctga caaatataat cctgaaggtc aaaaagtgac tacagaattg
      121 aataaggaac cagacgcatt tgagggaatt aagaacaaag aagatttacc aaaagatact
      181 aagtatactt ggaaagaaaa agtagatgtt agcgcagctg gaaataaaaa agggactgtt
      241 gtagtgacat atccagatgg atcatctgat gaagttgaag tagatgttac agtgacagac
      301 aatcgttctg acgccgataa atatgagcca acagtagaag g
//
LOCUS       DAZBSA010000291          339 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000291 DAZBSA010000000
VERSION     DAZBSA010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.300"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>339
                     /locus_tag="ACHGMT_002997"
     CDS             <1..>339
                     /locus_tag="ACHGMT_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN6836644.1"
                     /translation="EYLKINDTLEDILKKYEILANQKKITVNNYILDENVYIGKTALK
                     IILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIFDVKFDLNKENS
                     NGLGLYIVSN"
BASE COUNT          152 a           17 c           55 g          115 t
ORIGIN      
        1 aagaatattt gaaaataaat gatacattag aagacatatt aaaaaagtat gaaatattag
       61 caaatcaaaa gaaaataact gtcaataatt atatattaga cgagaatgtt tatataggaa
      121 aaacagcttt aaagattatt ctatctaatt tgattagtaa tgcggtaaaa tatactgatg
      181 taaatggggt aattaatatt gggatagtta atgattggtt atatatagag aattcatacg
      241 gtaacaataa aatttcgaat atggataaaa tatttgatgt taaatttgat ttaaataagg
      301 aaaatagtaa tggattaggt ttatatattg taagtaata
//
LOCUS       DAZBSA010000292          332 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000292 DAZBSA010000000
VERSION     DAZBSA010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.301"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            70..267
                     /locus_tag="ACHGMT_002998"
     CDS             70..267
                     /locus_tag="ACHGMT_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFN6836645.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            269..>332
                     /locus_tag="ACHGMT_002999"
     CDS             269..>332
                     /locus_tag="ACHGMT_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFN6836646.1"
                     /translation="MKKNAKQIVHELYNDISISKD"
BASE COUNT          125 a           32 c           67 g          108 t
ORIGIN      
        1 attgtttttt gaaatttaat gaatgttaga ataatagtct aaaaacatta atttaggggt
       61 gattagatta tgaaacattt aaaaattttg tctattaaag agacacaact tatctatggg
      121 ggtaccactc atagtggaaa atattatgga aatggagtgt attgcactaa aaataaatgt
      181 acggtcgatt gggccaaggc aactacttgt attgcaggaa tgtctatagg tggtttttta
      241 ggtggagcaa ttccagggaa gtgctaaaat gaaaaaaaat gctaagcaaa ttgttcatga
      301 attatataat gatatatcta taagtaaaga tc
//
LOCUS       DAZBSA010000293          330 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000293 DAZBSA010000000
VERSION     DAZBSA010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.302"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          111 a           56 c           55 g          108 t
ORIGIN      
        1 tggagtggta gttaattagt gacttatttt tgattttata gcactgatac tataaaatat
       61 agatatcatc atattacaca atcttaatac taacttaaaa aatatctcct ttcacaagta
      121 tggtgataaa attcgttccg ggctaccttt ttaggtagcc tactttaatc tttatacctt
      181 tctggatcaa cgaaagtata ctttatatag tcataacgcc gatgatcgct acgtgcgtcc
      241 ggcacgtcag tcatgaatcg gctacactag actagacaga aaaaataagg tgtgtagaat
      301 aataaaaaac acactggagg atttttcatg
//
LOCUS       DAZBSA010000294          329 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000294 DAZBSA010000000
VERSION     DAZBSA010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.303"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            264..>329
                     /locus_tag="ACHGMT_003000"
     CDS             264..>329
                     /locus_tag="ACHGMT_003000"
                     /inference="COORDINATES: protein motif:HMM:NF025639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836647.1"
                     /translation="MRHSNFLAFLFAPNETHNLSDE"
BASE COUNT          145 a           30 c           54 g          100 t
ORIGIN      
        1 gagaaattta taaataactt ttttatttgc tttggtagcc aagtaagttg caaaagaaaa
       61 atagatactg ttaaaaagat aatgtagaca tagaaacaaa tacgtaaaat tttagttaga
      121 ataatggcaa ggagaaaaaa catgatagat gaaaaaaagt gtttaaaaaa tctattagag
      181 gatatggatg atattgaaga aaactatcta aatggactgg gaaaacaaat atatttcaga
      241 tacttaagct atctaaaaat gaaataagac atagtaattt tttagctttt ttgtttgctc
      301 caaatgaaac ccacaattta tctgatgag
//
LOCUS       DAZBSA010000295          309 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000295 DAZBSA010000000
VERSION     DAZBSA010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.304"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..309
                     /locus_tag="ACHGMT_003001"
                     /pseudo
     CDS             1..309
                     /locus_tag="ACHGMT_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          122 a           61 c           59 g           67 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 gatgtgcaa
//
LOCUS       DAZBSA010000296          285 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000296 DAZBSA010000000
VERSION     DAZBSA010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.305"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          117 a           31 c           51 g           86 t
ORIGIN      
        1 gttctaaatg attcctttaa ggtgtcacta aaaattataa aaaatagtgt tgacgcactt
       61 ttgatgtcat gttataataa cgaagtcgtt tggatgagat agctaataaa gcttgatttt
      121 atcgaaaaaa acttcgaaaa agttattgac aaataacaat aaagatggta tgataatcaa
      181 gtcgctgcta atgagtggca aagacgttta gaaaaaataa acaaagaagt tgttgacatt
      241 ctcttctgaa tctgataaga ttaaagagtt gttaaaacaa aaaga
//
LOCUS       DAZBSA010000297          282 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000297 DAZBSA010000000
VERSION     DAZBSA010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.306"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>282
                     /locus_tag="ACHGMT_003002"
     CDS             <1..>282
                     /locus_tag="ACHGMT_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFN6836648.1"
                     /translation="QSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGK
                     KGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT           84 a           52 c           56 g           90 t
ORIGIN      
        1 aacaatcgaa gagacttttt cctgaaatca attgtgttgt cgctgatgct gggtataaga
       61 cacctaagtt tgttcacttt ttgactcatt tgaaccttcg accttgtttg ccttattcaa
      121 ggcccaaagg gaaaaagggg ctattatcaa aaaatgagtt tctctatgac gagtattttg
      181 attgttatat ttgtccgcaa gatcaaatgc tcgctttttc aacggttact cgagaagggt
      241 accgagaata caaatccaat ccaaaagaat gtgtgaactg tc
//
LOCUS       DAZBSA010000298          278 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000298 DAZBSA010000000
VERSION     DAZBSA010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.308"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            50..>278
                     /locus_tag="ACHGMT_003003"
     CDS             50..>278
                     /locus_tag="ACHGMT_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFN6836649.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVS"
BASE COUNT           86 a           51 c           53 g           88 t
ORIGIN      
        1 tgaaatcctt tagaattaat ttggctacca attcatagga ggattcaaga tgtctcatac
       61 tcagcttatc aaagataccc taaatattct agacctaaat atctatttcg aggaaaattg
      121 tttaactaaa gaaaaataca aagggcaaat ttgcttgatt tacaaaggaa ctcttctgta
      181 caagccagaa gagtgttccc attgtctttg tgtcgtcccc tctaggatca ttcgttgggg
      241 gacgacaacc gttcggttgt tactgaatga tgtgtctg
//
LOCUS       DAZBSA010000299          266 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000299 DAZBSA010000000
VERSION     DAZBSA010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.denovo.309"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(36..>266)
                     /locus_tag="ACHGMT_003004"
     CDS             complement(36..>266)
                     /locus_tag="ACHGMT_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN6836650.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           78 a           39 c           47 g          102 t
ORIGIN      
        1 atagaaggac tgaccttctg tctcagattt ttttgttatt tcacttgaat caattgattg
       61 atcaaaaaaa tcctgatttt catgttctca aatgatttaa atccataaga taatcgtttc
      121 agtgtcttga tatggggaat cttggcctct attttgccat tagaatacgg gtaaatcata
      181 gcattgtaaa taccttcttc atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca
      241 tcaagtgttt caggaagatt ttttaa
//
LOCUS       DAZBSA010000300        10002 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.guided.096, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000300 DAZBSA010000000
VERSION     DAZBSA010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.guided.096"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            177..1073
                     /locus_tag="ACHGMT_003005"
     CDS             177..1073
                     /locus_tag="ACHGMT_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFN6836651.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1223..2164)
                     /locus_tag="ACHGMT_003006"
     CDS             complement(1223..2164)
                     /locus_tag="ACHGMT_003006"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFN6836652.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2290..3411
                     /locus_tag="ACHGMT_003007"
     CDS             2290..3411
                     /locus_tag="ACHGMT_003007"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFN6836653.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3669..5171
                     /gene="eat(A)"
                     /locus_tag="ACHGMT_003008"
     CDS             3669..5171
                     /gene="eat(A)"
                     /locus_tag="ACHGMT_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFN6836654.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5238..5765
                     /locus_tag="ACHGMT_003009"
     CDS             5238..5765
                     /locus_tag="ACHGMT_003009"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFN6836655.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5976..7037
                     /locus_tag="ACHGMT_003010"
     CDS             5976..7037
                     /locus_tag="ACHGMT_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFN6836656.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            7040..7936
                     /gene="murQ"
                     /locus_tag="ACHGMT_003011"
     CDS             7040..7936
                     /gene="murQ"
                     /locus_tag="ACHGMT_003011"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFN6836657.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQVKLAEANGFVKGTL"
     gene            7950..9932
                     /locus_tag="ACHGMT_003012"
     CDS             7950..9932
                     /locus_tag="ACHGMT_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFN6836658.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
BASE COUNT         3292 a         1764 c         2181 g         2765 t
ORIGIN      
        1 caattacaac tattcagaaa acttttaggc gaattttaat agaagaatga tttatttcat
       61 catattttca tacaaatgtt aaattttcat acaaatatag gaaacgtttt actggctttt
      121 tactatttaa acaggctatc ataggaaaaa gtgacagaga aagtgaggcg gaaaagatga
      181 acaaaaatga cgtactgcta gttgcaaaaa ataaatcact ttattttttg ccttccatcc
      241 gctctttaga tgtttggtcc ggaaaagatt tactacagtc ggaattcatt aaatcagcta
      301 gacaacagtt ggcaaattat cagctgatcg tttttttaga ttatggattt gatatggaga
      361 tggctcgacg cgtgcgccca tttaccaatg caaaaattgt gttatttttt tggaatcact
      421 tcaaagcaga acacgaggcg atgctgaagc aggcacaaga tgaaccggca atcgatgaga
      481 tttatcattt tgattttttg gaagcaaaag aacttgggct gaaacataat agttcatttt
      541 attcgaaaca tatgaatttt tcttctagcg aaccaaagac ggatctattt tttggtgcaa
      601 cgaataatgg gagaaaagaa cgagcagaaa gctataagca ggaatttata aaaagagatg
      661 ttacgaccaa ctatttcatt cttccacatc gagggaatga acaacctgga tatcttactt
      721 atgaagagta cctgaaaaaa acaggggaat cgcgcgggat tctggaactg ttgcgggagg
      781 gtcagcaagg agtgactttg cggacatttg aatccatgta ttttcaaaag aaactagtga
      841 cggataatca agcgattgct cattatcatt tttatcgtcc tgaaaatatt tttttgcttc
      901 gagaacgtga tttagatgaa ttaccggaat ttctgcatac accatttaag ccaatcctat
      961 cagagctatt ggaattttat gatgcagaaa attgggcaaa acgatttatc actaacgatt
     1021 ccactattta tgagcaatac gaatatgacc tctcctctgt tgaaaacaaa taaaaaacct
     1081 acattttgaa gagattatgt actgaaacaa acaagcacaa ttcttatggc atcatgagag
     1141 ttgtgttttc tttttgatct tttattgggc ttaaaaaaag agaaccagct gctgattcag
     1201 ctgattctca tcagagagcc ttttattctg cgccaaggat tccttctttt gcatggacta
     1261 attgggtcaa aaaggcacaa tatggtgtag caatatcata tttttgtcct tttcgccaaa
     1321 tggcaccatt gatgtagtcg atttcagtca aacgatggtt tttgatcagg tcttggtaca
     1381 tagatgggta atgcaacccg atgttgtcag gatcataagt agaagcaata tgttggtaca
     1441 cttcttcttg atctagtatg attccttctt ttgctgcgat agaagcaaat tcagatacga
     1501 ttgttcgaac catgctctct gccgcttttt gcgcgcctag ttcagcaata ttacaatcca
     1561 aaatcgtgca taaaccattc aatgttccat ttacacaagc ttttcgccaa atggaataac
     1621 gaacgttatg gctatagatc ggatttaaat ttgcttcttg aaagacttct acaacttttt
     1681 ttgtaaatgc ttcgccttct ggttccaagt tctctagttc gatttctcca tcacctaaca
     1741 atgtgacttt tccgggacca gctagaccag ctgtccacat cgtgatccca aataagatat
     1801 ttttctttgg cacatatttt tccagtacat cttcatgacc taatccattt agtaaacaca
     1861 aaacataagt attgtcagta ataatggatt ggatcgaaca aaacatatca tctaattgat
     1921 tggcttttgt taatgctacg atcaagtcca cgtgttcgtt gctttcgatg atttcttctg
     1981 gtgaatagat tggcagtttt gcgaccactt ctttaccgtt aaagtcagca atcaacccat
     2041 ttgtacgaat ggcttcaatg tgagcaggcc aacgatcgat caagaggacc tcatttccgc
     2101 tttgatgtag catcaatccg attcggctgc ccattgctcc ggccccagct attgcgattt
     2161 tcatgttttt tctctccttt attcaatgag ttccctttta gtatatagaa aaatggaaga
     2221 aaatagcatt gttttgtttg aaaaaaatga aaaaaagtgt cttacttaag aagaaagtga
     2281 gggatacaga tggatatttt ggcggcaatt gattcattta aaggaagtgc ttcttcattt
     2341 gaactaaatc aggcagttct aaatggctgg gaagatcgta aaggaagaaa aatcaatgtg
     2401 ccgattgcag atggcggaga aaatactgca gaagcaatct attacgcgct caggggaaca
     2461 tggcgtacca ttgatgggat ggatctctta ttccgtcagc gaaaaatcag ctatttattg
     2521 acaacttggc aaggaaaaaa caccgcagtc atcgaatctg cacaagcatt ggggttagat
     2581 ctacttgata aaccaacgga tcaaactatc cgacttgctt ccagttatgc cttaggagaa
     2641 cttattaaag atgcactgct tcaagaagta gaacaaatta ttgtcacttt aggaggaagc
     2701 gggtgtagcg atggtggtct aggattactc caatccttgg gtgcttcttt agaagggtac
     2761 acagaaggaa atcctttatt atctactaaa aaacttcatt ttgatcgaag aaacgttctt
     2821 gtttctaaag taaaattgat tatcgctgca gatgttacca atccatatac aggtcaacaa
     2881 ggagctgctt ggcaatttgg aagacaaaaa ggaggaatag atacgacgct agctttctta
     2941 gataaacaag caggaaagat tgctcagcaa ataaaagatt tctgggaggt ggatttagat
     3001 gatattccag gaagtggagc agcaggcggt attggtggag cccttttttt gttgggagcg
     3061 aagatggttt ctggtttcga tttagtcagc caaatcactg gattggaaaa aaagatcgaa
     3121 caagcagatg tcatcattac aggagaaggg cggatcgatc agcaaacgat ccacggaaaa
     3181 gttccatacg gtgtagcagt gcttgcaaaa aaatatcgaa aaccaataat cgctatttgc
     3241 ggaagtagag accaagagct agaaggactt tctgattttc tcccaattgt cttaagtatt
     3301 catttgggtc caataggttt agaagaagca atggaacaca ggcaaacatt agaaaaaaca
     3361 aaaatcttag gacagacact tagcagattt ttcaccatcc tcccgaagta gttgacaaaa
     3421 tcattttctt tatgctatcg taatttcaaa tataggacaa agtgagggat ccgtgcattg
     3481 aaaaattaag tcgattgatg aaagctgcat tcagactgta ccttgtggag acaaggaagc
     3541 agtgtgcaga ttttcgggat tggcttggtt ttttcgatgt atgggtcttt ttgtgtgtat
     3601 ccaacccttg catcctcttt agccagttct gaaaatagga ctggtctttt tttacgaggt
     3661 gatggaaaat gtctaaaatc gaaataaaaa atctgacatt cggctacgac agccaaggca
     3721 cattattatt tgaacaagca aatctaaatt ttgacacaca atggaaacta ggacttatcg
     3781 gacgaaacgg tcgaggaaag acaactttac tgaatattct acaaaacaaa ctaccttatc
     3841 aagggcaagt aatccatcag caagaatttg cctatttccc gcaacagaca aaagataaag
     3901 aacgtttaac ctattacgtg ttaaatgata ttacggattt tgagatatgg gaaatcgaaa
     3961 gagagctcca attgatgcaa acagatcctg aaatcttatg gagagaattc agcacactat
     4021 cggggggaga gaagacaaaa gtcctactgg cacttttatt tgtggatgac actcatttcc
     4081 cgttaatcga tgaaccaacg aatcatttgg atatctctgg tagaaaacaa gtagcggctt
     4141 atttgaaaaa gaaaaaacaa ggcttcatcg tggtcagcca tgaccgggga tttatcgatg
     4201 aagtagtgga ccatgtttta gcaatcgaaa aaagtcaact ggaactttat caagggaatt
     4261 tctctatcta tgaagaacag aaaaaacttc gtgatgaatt tgaaatggct caaaatgaaa
     4321 aattgaaaaa agaagtcagt aggctaaaga aaacagcagc tgaaaaagcc gaatggtctc
     4381 gttcccgaga aggagataaa acaaagaaac aagtcggatt catcgatact gaatctagac
     4441 gagtgaataa aggagcagtg ggtgctgatg ctgcacggac gatgaaacga tccaaagcaa
     4501 tcgtgaatcg gatggagacc cagatcagcg agaaagaaaa actattaaaa gatatcgaat
     4561 atatcgattc tttgacgatg aatagccaag cgtctcacca taagcgactt ttaagcgtag
     4621 aagatcttca attagggtat gaaaatctgt tattcgagcc aattcatttt acaatcgagc
     4681 ctcatcagcg ggtggcgatt tcaggtccta acggtgcagg aaagtcatcc attatccatt
     4741 atcttctggg ggcattcaac ggcaaggtta taggagaaaa ataccagcca aaacatctga
     4801 gcattagtta tgcaagccaa aattatgaag acaatcgagg aacgttggcg gaatttgcag
     4861 agaaaaacca agtagactac caagcatttt tgaacaacct ccgaaagctt gggatggaaa
     4921 gagatgtttt tcataacaag atcgagcaga tgagtatggg ccaacggaaa aaagtggaat
     4981 tggctaaatc tttatcacag ccagctgaac tatatacatg ggatgaacca ttgaattatt
     5041 tggatgtctt caatcaagaa caattagaac aactgatctt gaacgtgaaa cctgccatgt
     5101 tactagtgga acatgatcaa accttcctgg ataaagtatc tactgagatt atttctcttg
     5161 agagaatcta acccttgtaa tctcaagaac aacaaggtat ggttaagggg aactttgcca
     5221 acgaaggaga gaatcacatg ctttatgtaa cgagacacgg agaaacaact tggaacgcac
     5281 aaggattagt ttgcggacgg gcagatgtgc cgctgactga aaaaggacag atgcaggcac
     5341 agaaactagc agaaaaggta gtagatttgc ctgttccaat cacgaaaatc atccattccc
     5401 ctcttcaacg agcaagagat acagcacaag cagttgctga tcgtctcagc ttgccgctga
     5461 ccgttgatga acgattggta gaaatggatt ttggggatta tgatggactg ccaagcaaag
     5521 atgagaactt tcaaaaagca cgtttagcgt tcgctgtacg attcccaaat ggggagtcag
     5581 tcttagatgt gtatgcacgg atcgttcctt tattgaaaga atgcatcgaa gacgaagaaa
     5641 atgtctatct gttagtttgc cataatgcct tgataagagt gatcaatgct tattttcatc
     5701 cgatgcctaa tgaggggttc tttacattca tggtagataa tacagaattg gtttcttatg
     5761 agtaattatg tttttgtgtt atttaaataa aatatttcaa ggcactgctt ttgcttagca
     5821 gtgccttttt taggttatga caattctttt gataatataa actcaacctt tagaaatgaa
     5881 atttaattat tttaataaaa gtattgaaat tttgtttctt tagatatata ctctagtcat
     5941 agaaatgaag acggatacag aaaaagaggg ataatatgtt tggcttttca atatttatga
     6001 acgattcttt gtcagaagag aagaaacagt atatccgcca aatggcatcc aatggctttg
     6061 tcggtatttt cacttccatg catataccag aagatgatgt ttcggcatat caaaagcgat
     6121 tgatcgaact aggaagcgaa gcgaaaaaga ataaactaga attgatggtc gatatttcag
     6181 gagaggcatt ggaacgagcg ggtttttcat tagaagatct aacgcctttg aaagcaattg
     6241 gtgtaacagg tttaagaatg gattatcata tttccaatca gcagattgcc gactggtcac
     6301 atcaattgaa aatcagtttg aatgctagca cgatcacgcc taaagatatc gatgaattga
     6361 aagaagcaga agctgatttc tcacagattg aagcttggca taattattat ccacgtcccg
     6421 aaacagggct ggacaaagaa tggtatcaga aaaaaaatca atggctcaaa attcaaggtc
     6481 tccttgtcca aggttttgta ccaggagata cagagttgag aggcccacta tatcaaggat
     6541 tgccaacttt ggaagaacat cgagggatcc atcctcttgc aggcgctcta gatctattag
     6601 caagtaacac ggatattgtt tacatcggag atgcaggtct gtcagaaaac gttcaggagc
     6661 aatttgcttc tttccaaaaa gaacagacag tcttgctcca tacggaacct gtagatgaag
     6721 aattttacga atatatatta gggaagcata cgaatcggca agacgatgca agagatgtca
     6781 tacgaagcgt agatgcacgt tttcgagaga ttccgcctat cccagcaaga aatacagcta
     6841 cgcgtatgaa agggtctatt actttagata acgaaaagta tttgcgctat atgggtgaga
     6901 tccaattaac caaatatgat ttgccagcgg atgaaaaagt gaatgtagta gctaaagtta
     6961 tcaaagaaga gctaccattg atcaaccaaa tcaaagctgg tatgaactat caatttataa
     7021 gaaaagaagg aagataaaaa tgagcaaaat caatttagag aacctaacaa cggaacgcag
     7081 aaaccaagaa acttttggat tagatgagat gagtatttct caagccgttc aactaatgaa
     7141 caaagaagat cagaaagtgg ccgctgcggt tgaaaaagaa ctgacaagta tcgaaccagt
     7201 gattgctaac acgattgagt cctttaaaaa aggtggacgt ctgatttata tgggagccgg
     7261 aacaagtgga agactaggtg tgttagatgc tgctgaatgc gtaccgacat tcggtgtaga
     7321 accggaaatg gtcataggtc taattgctgg tggagaagaa gcaatgactg tagctgtcga
     7381 aggggccgaa gattcttatg agctagggca agaagatcta atcaagctga atttgacaaa
     7441 aaatgatata gttattggta tcgcagctag cggtcgtaca ccatatgtga tcggtggatt
     7501 agattacgca aaatcgatcg gtgctgtcac aggaacgatt tcttgcaata aacaagccga
     7561 aatcagtaaa cacgcggatt tcccaatcga agtcgatgca ggtccagaat ttttgacagg
     7621 ctctactcga ttgaagtcag gaacagcaca aaaacttatc ttgaatatga tttctactat
     7681 cagtatgatt ggtataggta aagtattcaa taacttgatg gtcgatgtca aaccaacaaa
     7741 tgaaaaacta atcgaacgct caaaacgaat catcatgcaa gcgacagaag ctgattacga
     7801 aacagctgaa aagtactttg aagaagcaga tcaaaatgtt aagctggcta tcgtcatgat
     7861 tttgacaaat agtgataaag aggaagctca agtaaaatta gctgaagcaa acggatttgt
     7921 aaaagggacc ttatgagggg gaaaagacca tggcaaaaaa cgaactgact ttggaagaac
     7981 tggcaaaaca aatttataaa ggggcaggcg gcatgggcaa tgtagaaagc attgtccact
     8041 gcatgactcg tgtgcgtatg aagatcatca atgatgatct tgtaaatgtc tctgaattaa
     8101 aagcgattcc aggagtgctt ggtgtagtag aagatgaaca gcttcaagtc atcatcggac
     8161 ctgggaaagt gaacaaagta gcacagcaaa tggttgattt ggcaggcgtg aaattaggag
     8221 aaaaactttc ttctgtaagt acagctacag caaattcatc agcatctgga aaagaccgtg
     8281 taaacgaacg ggcacaagaa atgaaagcgg cacaaaaatc gaaacaaaaa ccatctaaaa
     8341 tcaaatcggt attaaaagac atttctaata tctttgtgcc aatgattcct gcatttgtcg
     8401 gtactgggat cgtggcaggg attgccgcag ttttagcaaa cttagtcaca gccggaacac
     8461 ttgatgcagc aacatggcaa caatatatcg atgtgatgaa catcttgaaa aacgggatgt
     8521 ttagctacct agttatctat acaggtatca atgctgcaca agtttttggt gcaacaccaa
     8581 ctcttggtgg agttatcggg gccgttgtga tgctgacagg gatgaatccg gaagcaccac
     8641 tgaaaaactt atttactggt gaagctcttg cagctggaca aggtgggatc ctaggtgtca
     8701 tcttcgctgt ttggctattg tcaatcgttg agaaaaaact gcataaagta gtacctgatg
     8761 cggtagatat catcgttaca ccaactcttt cattgattgc catcggtctg attgaaattt
     8821 tcttcatcat gcctttagca ggtttcattt ctgatggcat ggtcggtggg atcaactggg
     8881 tacttggcgt tggtggagca tttgccggct tcgttctagg cacattattc ttgccgatgg
     8941 ttatgttcgg actgcatcaa attttgactc caatccatgt gcaaatgatt gatgaaactg
     9001 gtcgtacgct attgcttcca attcttgcga tggctggtgc aggacaagtg ggtgcagcat
     9061 tggcattatg gattcgctgc aaaaaagata aagaattaac agaaatgatc aaaggagctt
     9121 taccagtcgg gatcctagga atcggcgaac cattgattta cggagtaaca ttacctctag
     9181 gacgcccatt tattactgct tgtatcggtg gaggtatcgg cggggcagtg atcggaatga
     9241 tcggaaatgt cggggctatc gctattggac cttcaggagc cgctttgatc ccattgatct
     9301 ctgacggtaa atggttaggc tacatccttg gattattggc agcttatgct ggtggcttcg
     9361 ttgctacatt cttcttcggt attcctaaag accgtttaga aaaagaagaa ttagcgactg
     9421 aaacagccac agtaaatgaa gtgcctgaaa aagtagtacc tcaaacttca gctgaaagtg
     9481 tgactatcct ctcatcagtt gctgacggaa cagttgtttc tttagataaa gcaagtgatc
     9541 cagtttttgc acaaaagatg atgggtgaag gctactttgt agaaccagaa aacggacata
     9601 tctattctcc agttactggg gtagtcagct ctgtcttccc aacgaaacac gctattggga
     9661 tcacaacgcc aaatggttta gaaatcttgc ttcatatggg aatcaacaca gttgacttag
     9721 gcggtacgcc atttgatgtg aaagtaacag aagggcagca agtgacacct gatacgcttg
     9781 tagcagaagc tgacttagaa gcgatcaaag cagcaggcaa agaaacatcc atgatcgtct
     9841 tggtaaccaa tatggatcgt gtaaaaaact ttgtcttgga aaaaacaggt aaagcaaaag
     9901 caaaagcacc tgtgatggat gtcaaagctt aatatgatag aaacaataga aacgaaagaa
     9961 gaaactgaaa tctatttttc agcttcttct tttttattat tt
//
LOCUS       DAZBSA010000301         4960 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.guided.138, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000301 DAZBSA010000000
VERSION     DAZBSA010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.guided.138"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..556
                     /locus_tag="ACHGMT_003013"
     CDS             2..556
                     /locus_tag="ACHGMT_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFN6836659.1"
                     /translation="MPHEEMLEMIKEATTAPSSVNMKPWRFVVAESEEAKEILRPLIR
                     FNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAYESGKMPKEVRDQQLAAIIPYYKSFS
                     REEMNDVVKVDSSLAAMQFMLVARDHGYETNPIGGFEADQLAEAFGLDKDRYVPVIIL
                     SIGKAVEEGYESVRLAPEKITMFE"
     gene            910..2388
                     /gene="msr(C)"
                     /locus_tag="ACHGMT_003014"
     CDS             910..2388
                     /gene="msr(C)"
                     /locus_tag="ACHGMT_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFN6836660.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            2435..2881
                     /locus_tag="ACHGMT_003015"
     CDS             2435..2881
                     /locus_tag="ACHGMT_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFN6836661.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3080..3595)
                     /locus_tag="ACHGMT_003016"
     CDS             complement(3080..3595)
                     /locus_tag="ACHGMT_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFN6836662.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            3712..4914
                     /locus_tag="ACHGMT_003017"
     CDS             3712..4914
                     /locus_tag="ACHGMT_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN6836663.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1507 a          833 c         1079 g         1541 t
ORIGIN      
        1 aatcccacac gaagaaatgc tggaaatgat caaagaagcg acgactgcac cttcttctgt
       61 caatatgaaa ccatggcgct ttgtggtcgc tgaaagcgag gaagcaaaag aaatcttgcg
      121 tcctttgatc cgtttcaata cgagacaaaa tgatacttct tctgcaatgg tcatgatttt
      181 tggcgatatg caatgttacg aatatggcga agaaatctac aaccaagctt acgaatcagg
      241 gaagatgcca aaagaagtca gagatcaaca attagctgcg atcattcctt attacaagag
      301 tttttcaaga gaagaaatga acgatgtagt caaagtcgat tctagcttag cggctatgca
      361 attcatgtta gttgcgcgtg atcatggcta tgaaacaaat ccaattggtg gattcgaagc
      421 agatcagttg gcagaggcat ttggtttgga taaagaccgt tatgttccag ttattatttt
      481 atcaatagga aaagcggtag aagaaggtta cgagtctgtt cgtttagcac cagaaaagat
      541 cactatgttc gaataaaagg ttttcagtta ctattcagta gctattgctt tttttgaatc
      601 gtgtgtggta acctaataaa aaaggaggtc ggaaaaatga ctgcatcgat gaaattacgt
      661 ttcgaacttt tgaataacaa ctaaataaat tcaaaagaca tgtataaaca acaatttcat
      721 ggatggagta tgtccatttt ttcgaacgac agtagaatag gtgaaagcta tgacagattt
      781 ggtgatttag tcatagttat ttcgttcaga gtaggaaaag atgtgatgca gtttttttgc
      841 aaacagattt ttctgctctt ttttagtatc gataattgtt gttttacatg aaataggagt
      901 gtaaaacata tggaaaattt agcagtaaat ataacaaatc tacaagtcag ctttggcaac
      961 cagctagaac tatctattga ttctcttcgt gtctatcagc aagatcgtat agggatcata
     1021 ggagaaaatg gtgtcggtaa atcgactttg ctcaaactaa tagccggtga actttttccc
     1081 gatcatggga aaatccaaac agagatcacc ttcaactacc tgcctcaatt aacctatctc
     1141 gctgaagcaa aggacctaaa tttggaatta gccagtcatt tccagttaag actggaagaa
     1201 acttcagagc ggaaatggag cggaggggaa gaacgaaaga tcgagttgat acgtcttctt
     1261 tcttcttatg aacaagggat gcttctagac gagccaacaa cccatctaga tagaaaaagt
     1321 attgatcgac tgattgaaga gcttcgttat tattatggta cgctggtttt tgttagtcat
     1381 gatcgctatt ttctagatga gctagcatcg aaaatctggg aagtaaaaga cggagaaatc
     1441 cgagagtttt cggggaatta tagtgcctat ctcactcaaa aggaattgga gaaaaagact
     1501 cagctacgag aagcagagtc gatcatgaaa gagaaaaaac gattggaaaa atcgatccaa
     1561 gaaaagaaaa aacaagcgga aaagttggaa aaagtgtcca gtaaaaagaa aaagcaacaa
     1621 atcagactgg atcggttgtc ttcctctaaa caaaaagaca gtgtacaaaa agccatccaa
     1681 aagaatgcga aaacattaga gagaagactc caaaaaatag gagaaacaac caagccgcag
     1741 cagatgaaac aaatccgttt tccagtacca aaatctcttg aactccacag ccgctatccc
     1801 atcatgggac aaaatgtcca attggaaaga agtgggagaa cattactggt aaatggtgat
     1861 tttcagtttt ctttaggtaa aaaaatcgcg attgtcggtg aaaatggttc aggtaagaca
     1921 accttattag aacatatccg caaacaagga gaaggaatcc ttctctctcc gaaagtaagc
     1981 tttcaagtat atcagcaaaa gggttatcaa atgacatctg aagaatccat cattcgtttt
     2041 gtcatgagac aaacagagtt ttcagaatcg cttgtccgta gtttgctgaa tcacttaggg
     2101 tttgctcagg aaactttgac gaaaccgtta tgtacattaa gtgggggaga agcgacccgt
     2161 ctgacgattg ctttgctttt tactaagcca agtaatgtgt tgctgttaga tgaaccgact
     2221 aattttattg atatggcaac gatcgaagct ttagagaagc taatgcaaat atatccggga
     2281 acgattttgt ttacttctca tgattcttac tttgtcgagc gtacggctga tgaagtttat
     2341 gaaataaaag ggcagaaaat aaaaaaagta cttacgagaa atttttaaat gtgctacgat
     2401 gattttcgtg acaaatttaa aggagtggta cagattgcct acaaataaaa aagaaggaat
     2461 aatttttaca acaatgatgt gttttttgat ggtcttgggg atgagcgcgt ataatttatg
     2521 gctgcatcaa gatcttcatt tatcaagttt gttgattggt ctagtgcctg gattcgtggt
     2581 ggctttcatt ttggatgtct tcatcatcgg tgtgctggcg aaaaaaattg cctttaaact
     2641 accagtaaat ccagaaagca aggtccaaat gatcttaact atttcatgct tgatggtttt
     2701 aggaatggtg acatgtatgt ctttattcgg tgtgatcatg gaacaaggaa tacctgatca
     2761 attattctct gcttatatga atgcttggaa aatgaatgtg attgccgctt taccgttgca
     2821 gttgatcatc gttggcccgc tttcaagaag tgtgctaaaa gcaattcaaa gtaatgaatg
     2881 atggaacact gttatttgaa aaaaggcctt ggcatgcttt ttgtcatagt ctcttgaatg
     2941 cagttctaga aaaatagtga tccgaacgat aggttttggc ctgtcgttcg gatcgctttt
     3001 tcttttccaa ctgtattgct cttttattga agataatttg ctcttcaata gatatttcta
     3061 gaaaggttct gtttatccct tactcaaaaa actgctgcat ttgttctctt acttgctgac
     3121 accattggag ttcctgctga caaagcataa ttctgcgctg gatcacaaca taacgtccaa
     3181 attcttgttt cttgtctagc gagaggtcat tcaatttttt ttgatttttt tgcatgattt
     3241 tgttcagctg ttgttctcta gtgaatagca gtccagtagc tgttgtttta tcgagcgtcg
     3301 aaaaagcata gatttttgct agaaattcat ctttttgcgt aggagcggga gtttcctctt
     3361 tgatccattc ctctacaaga gcacgtcctt catcagtcaa gtggtagatc tttttttgag
     3421 agtgctcgtc tttgtccaaa acggtcacat aaccctgctg gtctaacttg gcaagcgttg
     3481 tatagatctg actgtgatgg gcttcccaaa aaattgtgat cagctgcttc aattcatacc
     3541 cagaacgtgg tttggataaa agcacgcata ataatatata ggctaaagta ttcataaaaa
     3601 tcctacctct cgtcttattt tctttattat atcacagtaa agtttccacg ttttacatgt
     3661 gcatattgac atgtaaaata aatcatgtat gatgctaata gagagaaagg gatggaaata
     3721 ttggagaatc aaatagatat acaagaacaa acaaatgaag aaacaaaggg gaaaagttca
     3781 gcgctagttg tagcagctac ctgtatgatc gcttttatgg gagtgggtct agtagaccca
     3841 atcttaaaaa cgattgctgc acagttaaac gctacgccag ctgaaacgac cctgttattt
     3901 actagttaca tgttagtcac tggtgtggtg atgctattta caggtttctt atctagtcgg
     3961 atcggtttga aaaaaacatt gatgacagga ttgtttatca tcgttgtatt cgctggtctt
     4021 ggcggattat ctgggaatat cggaatgctg attgggttac gtgccggctg gggtgtcggg
     4081 aatgctttat ttgtctctac tgctttagca acgatcgtca gtattttagt tggtaacaca
     4141 gaaaaagcaa tcatgatgta tgaagcagca cttggctgcg gaatggctgt tggccctcta
     4201 gtcggtgggg tcttgggcag tggttcatgg cgttatccat tttttggggt agcatttttg
     4261 atgttttgcg gttttatagc cctagcggta cttttacctg aaactgagaa accaaagaga
     4321 aaagtaggcc tttgggaagg tgtcaaagcg ttaagcaatc ataaacttcg ttcagttgga
     4381 ctaatcgcat tgttatataa tttcggattc tttactttac ttgcgtatgc gccattttta
     4441 ttgattggct attctgaatt agaagtcggt tttgtatttt ttggctgggg tgtgcttctg
     4501 gctattgctt cgattttcgt tgcaccggct cttgagcgga agtttacgac ttatcggaca
     4561 attttagcag catttgtctt attcattatt tgtttgctgt tattaggagc tggaacttct
     4621 atgccagtcc ttgtaccgat cagcattgtg ttagcaggat ttttccaagg aattatcaat
     4681 acactgttga cgacgattgc tatggaaata ccaggtctgc aacgaaacgt tgcttcgtcc
     4741 agctatagtt ttgtccgctt tttcggtgga gctttagcac catttattgc aggtgtgatt
     4801 ggtgaacata ttggtgaaag ctatgcgttt tattttgcag gtgccattgt tcttatcagt
     4861 gtatgttgta tcatttatca ccgtaattat tttgctacaa atgaagaaga ataagaaatc
     4921 agaggttgtg aaaaagctgt cctgcatcaa gtcataagaa
//
LOCUS       DAZBSA010000302         4703 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.guided.146, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000302 DAZBSA010000000
VERSION     DAZBSA010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.guided.146"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            194..1000
                     /locus_tag="ACHGMT_003018"
     CDS             194..1000
                     /locus_tag="ACHGMT_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFN6836664.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            1018..1725
                     /locus_tag="ACHGMT_003019"
     CDS             1018..1725
                     /locus_tag="ACHGMT_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFN6836665.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            1722..2693
                     /locus_tag="ACHGMT_003020"
     CDS             1722..2693
                     /locus_tag="ACHGMT_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFN6836666.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            2686..3715
                     /gene="vanB"
                     /locus_tag="ACHGMT_003021"
                     /pseudo
     CDS             2686..3715
                     /gene="vanB"
                     /locus_tag="ACHGMT_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
     gene            3721..4329
                     /locus_tag="ACHGMT_003022"
     CDS             3721..4329
                     /locus_tag="ACHGMT_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFN6836667.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(4395..4568)
                     /locus_tag="ACHGMT_003023"
     CDS             complement(4395..4568)
                     /locus_tag="ACHGMT_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836668.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         1329 a          986 c         1245 g         1143 t
ORIGIN      
        1 gatagaatga ttttctatca gtcctagaaa ttatagagcc gattttgacc gacaggtata
       61 accctgccgg tctttttttg ctgctacagg aaaactacag actggctaca gggaaagtac
      121 agatgcgctt gccataataa caatcgtacc agccacaaat cgtagtttta ttgcaaagga
      181 ggcattcgat caaatggaaa aaagcaacta tcattccaat gcggatcatc acaaacggca
      241 tatgaaacaa tccgtggaaa agcgggcttt tctatgtgcg ttcattattt cgttcacagt
      301 ctgcacgctg tttttggggt ggagactggc ttccgtattg gaggcaacac agataccgcc
      361 catccctgca actcatacag gcagcagcac tgacgtagtg gagaatttgg aggaaaacgc
      421 tcttgccacc gccaaagaac agggagatga acaggaatgg agcctgattt tagtgaacag
      481 gcagaacccc atccccgcac agtacgatgt ggagcttgag caactatcaa atggtgagcg
      541 gatagatatt cggatttctc cctatcttca agatttgttt gatgccgcaa gaactgatgg
      601 agtttacccg attgtcgcat ccggataccg aacaacagaa aaacagcaag aaattatgga
      661 tgaaaaaatt gccgaatata aggcgaaagg ctacacctct gcacaggcta aagcggaagc
      721 agaaacttgg gtggccgtgc cgggaacgag cgagcatcag cttggtcttg ctgtggatat
      781 caatgcggac ggaattcatt caacaggcaa cgaggtttat agatggctgg atgaaaacag
      841 ctatcgtttt ggttttattc gtcgctaccc accagacaag acagagataa ccggtgtgag
      901 caacgagccg tggcattacc gatatgttgg catcgaagct gccacagaga tgtacaacca
      961 aggggtttgc cttgaggaat atttaaaacc agaaaaatga gaaaaggatg ttatgttatg
     1021 gacagaaaaa gattgacaca gcgtttcccg ttcctgcttc caatgagacg ggcgcagaga
     1081 aaaatgtgct tttatgcggg aatgagattt gacggctgtc gctatgcgca gacgataggg
     1141 gaaaaatcgc tttcccattt gctctttgaa acggattgcg cattatacaa ccacaatacc
     1201 ggatttgaca tgatatacca ggaaaacaag gtgttcaact taaagctggc agcaaagacc
     1261 ttaaacggct tattgataag accgggggaa accttttctt tctggttgtt cctgcatacg
     1321 ccattgacaa ttatccagcg cagaggtcac gaagtaaagg agtttccaga gccaaacagt
     1381 gacgagatta aaggggtgga tgcaaccatt tcagagggct ggattgattt aaaagtgcga
     1441 aacgataccg actgcaccta ccaaatatgg gtgaccttag atgatgagaa aatcatcggt
     1501 caggtgttcg ccgacaaaga gccccaagca ttatacaaaa ttacaaatgg cagtatccag
     1561 tatgtccgtg aaagtggcgg gatttatgaa tatgcccagg ttaaacggat gcaagttgcc
     1621 ttaggtaccg gggaaataat agattgcaag ctgctttata caaacaaatg caaaatctgt
     1681 tatcccctcc cggaaagtgt ggatattcag gaggagaacc aatgagaaaa agtgtgggca
     1741 ttactgtttt tggatgcgaa caggatgagg caaatgcttt ccgcgcttta tcgccggatt
     1801 ttcatattat ccctacgctg attagcgatg cgatatcggc agacaacgca aaattggccg
     1861 ctggcaatca atgcgttagc gtaagccata agtccgaggt ttccgaggcg acaattcttg
     1921 cgctgagaaa ggtcggggta aaatacattt ctacccgcag catcggctgc gatcacattg
     1981 atacgactgc cgccgagaga atgggaatct cggttggcac ggttgcgtat tcgccggaca
     2041 gcgttgcgga ttatgctttg atgctgatgc tgatggccat acggggtgca aaacccacca
     2101 tgcacgccgt ggcgcaacaa gatttcagat tggatcgtat ccgggggaaa gaactggggg
     2161 atatgactgt gggagttatt ggaaccggcc atatcgggca agcggtcgtc aaaaggctgc
     2221 ggggatttgg atgccatgtg ctggcctatg ataacagccg aaaaatggat gcagattatg
     2281 tccagcttga tgagcttcta aaaaacagcg atattgttac gctccatgtg ccgctttgtg
     2341 cggatacccg ccatctgatc ggtcagaagc aaattggaga gatgaagcaa ggcgcatttt
     2401 taatcaacac cgggcgcgga gcgcttgtcg ataccggggc gctggtggag gcgttggaaa
     2461 gcggaaagct gggtggtgcg gcactggatg tgttggaggg cgaggatcag tttgtttata
     2521 ccgattgctc gcagaaagtg cttgaccatc cctttttgtc gcagctttta aggatgccga
     2581 atgtgatcat cacaccccat acggcgtact acactgagcg tgtgctgcag gatactacag
     2641 aaaaaacaat caggaattgt ctcaattttg aaaggagttt acagcatgaa tagaataaaa
     2701 gtcgcaatca tcttcggcgg ttgctcggag gaacatgatg tgtcggtaaa agccgcaata
     2761 gaaattgctg cgaacattga tacggaaaaa ttcgatccgc actacatcgg aattacaaaa
     2821 aacggtgtat ggaagctatg caagaagcca tgtacggaat gggaagccga cagtctcccc
     2881 gccatactct ccccggatag gaaaacgcat gggctgcttg tcatgaaaga aagcgaatac
     2941 gaaacacggc gtattgatgt ggctttcccg gttttgcatg gcaaatgcgg ggaggatggt
     3001 gcgatacagg ggctgtttgt attgtctggt atccccttat gtgggctgtg atattcaaag
     3061 ctccgcagct tgcatggaca aatcactggc ctacattctt acaaaaaatg cgggcatcgc
     3121 cgttcccgaa tttcaaatga ttgataaagg tgacaagccg gaggcgggtg cgcttaccta
     3181 ccctgtcttt gtgaagccgg cacggtcagg ttcgtccttt ggcgtaacca aagtaaacgg
     3241 tacggaagaa cttaacgctg cgatagaagc ggcaggacaa tatgatggaa aaatcttaat
     3301 tgagcaagcg atttcgggct gtgaggtcgg gtgtgcggtc atggggaacg aggatgattt
     3361 gattgtcggc gaagtggatc aaatccggct gagccacggt atcttccgca tccatcagga
     3421 aaacgagccg gaaaaaggct cagaaaatgc gatgattaca gttcccgcag acattccggt
     3481 cgaggaacga aatcgggtgc aggaaacggc aaagaaagta tatcgggtgc ttggatgcag
     3541 agggcttgcc cgtgttgatc tttttttgca ggaggatggc ggcatcgttc taaatgaggt
     3601 caataccctg cccggtttta catcgtacag ccgctaccca cgtatgatgg ccgccgcagg
     3661 aatcacgctt cctgcactga ttgacagcct gattacattg gcgttaaaga ggtgataagt
     3721 atggaaaatg gttttttgtt tttagatgaa atgttgcatg gcgtccgttg ggatgccaag
     3781 tacgccacat gggataactt cacaggaaaa ccggtggatg ggtatgaggt gaatcgcatc
     3841 atcggcacaa aggccgtggc gtttgctctg cgcgaggcac aaatccatgc ggctgcgctt
     3901 ggctatggct tgcttttatg ggatggatat cggccaagaa ctgcggtgga ctgcttcctg
     3961 cgttgggcag cgcaaccgga ggacaatctc acaaaagaaa aattttaccc caatatagag
     4021 cgagccgagt tgattacaaa gggttatgtg gcctcacaat ccagccatag ccgtggaagc
     4081 gcaattgatc ttacgctcta ccacctggat acaggggaac ttgtttcaat gggaagtaac
     4141 ttcgatttta tggacgaacg gtcgcatcat acagcaaaag ggatagggga tgcagaggca
     4201 caaaatcgaa gatgcttgcg taaaatcatg gaaagcagcg gatttcagtc ttatcgcttt
     4261 gaatggtggc actataagtt gattgatgag ccataccccg atacctattt taattttgcc
     4321 gtttcataat gaaagtattg gttttgtatc agattttgga tttgataaaa tctatgctgg
     4381 tcatggacaa tcttttactg gaaagtggtt aaagcatctt ttgtaataca ttgcaaatta
     4441 tagtattcaa tctgcccagc ggcagaaaag aaaaaaaccg ctgttgggca gacctttccc
     4501 acgcccattt tgtattttgc tggcggcggt tttgagaaat caaggccgcc gttcttttct
     4561 ggcagcaaaa ggtgtggcgg cccataggag gatgccgcta tgctgttttg ctattgccat
     4621 gctctaatcc acttccacca ctcaaaaaaa gccttgcccc tccatgaact gaaccccaaa
     4681 agttgaacta tttaatggac tgt
//
LOCUS       DAZBSA010000303         4454 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.guided.151, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000303 DAZBSA010000000
VERSION     DAZBSA010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.guided.151"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            144..641
                     /gene="dfrG"
                     /locus_tag="ACHGMT_003024"
     CDS             144..641
                     /gene="dfrG"
                     /locus_tag="ACHGMT_003024"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFN6836669.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(713..2665)
                     /locus_tag="ACHGMT_003025"
     CDS             complement(713..2665)
                     /locus_tag="ACHGMT_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFN6836670.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2672..2908)
                     /locus_tag="ACHGMT_003026"
     CDS             complement(2672..2908)
                     /locus_tag="ACHGMT_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836671.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            3310..3600
                     /locus_tag="ACHGMT_003027"
     CDS             3310..3600
                     /locus_tag="ACHGMT_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836672.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(3872..4221)
                     /locus_tag="ACHGMT_003028"
                     /pseudo
     CDS             complement(3872..4221)
                     /locus_tag="ACHGMT_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
BASE COUNT         1415 a          738 c          783 g         1518 t
ORIGIN      
        1 aaaatttgtg attttgtggt attatgataa tataacttat agaaaaaatt gcttattcaa
       61 ctaacgggca ggataatgag aaaaggtttt ttgatagaaa taagaaatta gatggtaaat
      121 tagttaaaaa ggctgtgtta taaatgaaag tttctttgat tgctgcgatg gataagaata
      181 gagtgattgg caaagagaat gacattcctt ggaggattcc caaggactgg gaatatgtta
      241 aaaatactac aaagggacat ccgataatat taggtaggaa gaaccttgaa tcaatcggaa
      301 gagccttacc tgacagaaga aatattattc tgacgagaga taaggggttt acctttaatg
      361 gttgtgaaat tgttcattca atagaagatg tttttgagtt atgtaaaaac gaagaagaaa
      421 tttttatttt cggaggagaa cagatttata atttgttttt cccttatgtt gagaaaatgt
      481 acatcacaaa aatacatcat gaattcgaag gagatacttt ttttccagaa gtgaattatg
      541 aggaatggaa tgaggtattt gcccaaaaag ggataaagaa tgataaaaat ccgtataact
      601 actattttca tgtatatgaa agaaaaaact tattgagtta acgggtgcgt taattgaaga
      661 agaacagtaa agataaagca caagagcttg ggattactcc aagctctttt tgtcatttga
      721 aataaattat atctttccaa tcaaattgtt tatctgtaat ccctaatctt tgagcaggtg
      781 tcaaaacctt tttatccgct gatttaaaag ggcgacaaaa gttgtagtaa gttcttagaa
      841 ttgtaatcgc aaattgagca tacttcggat tgaagttagc gtaaatatag ctcttcttat
      901 caccacgagc tgttgttaat ggtctttcaa gagatgatat ataacgtctt atctgttgaa
      961 taaagctatt ggtcgaatgg tcattaacat ttaatagcat ctttgcgatt tcgtttggtt
     1021 ccagagcaga taaatccgtt ctacaatcaa ctgaataaaa tcctttatcc ttagtggcta
     1081 atggatgttt gatagggtta tcggcatatt tgcgatatct ttctccatct ttactaatag
     1141 cctcttcgtg gaattgatga cttgttagta attcagttag ataatgagat gccaaagtcc
     1201 gtagaaactt tgttttgaat cctctgatat ctccccaatc gagcagttct gcttttgctt
     1261 gtccgaattc ttttaagcat tgttttctgc ttttcgtctt gttaacttgg caaataaaat
     1321 gatgtgcatc gctcaacctt aattctttcg tgaatactct ataaaaggcg tttctaatag
     1381 agaagtcatc atcactaatc attcgccatt cgtcggcatt aaccatttgt ttaattaacc
     1441 aatagtgagc agttgtagta taaggagcat caacatttaa accatcgaca aatctactcc
     1501 tattagtaat tttccctaat tcgtgaagat aagcattcct attttcgcta tcgttagcgg
     1561 atggttcttg tggataataa gaccaatcta acctatcatt tttacgacta aaagtattga
     1621 gatggtcttc tttgaatttt cttgtatctt catttaattc atccattgaa atattccaat
     1681 cataggcaac atcagaacgg aacacatatc ttgataagac ctcagcagat acaactatat
     1741 acgtttgcat attaaggtct tcaaaccccg tatatttctt tgaaccttgt cctttcttac
     1801 ggacgttatt taggtagtag tgcatcttgt ccgtattaag ccacatctcg ttaaatttct
     1861 ttgtttgaag gggttgtgtc tcatatcgtt ctaaaaactc caaacaacgc ctatacagcc
     1921 actccagctt atgatagtag gttccaactc caattcctaa aatatcacaa gtacgactga
     1981 ctgaaaccct tccaacaacc atcttagcga acattggaag aatggtgttc ttttgttggt
     2041 gataagtagt ggtttcttct ctttttggta gaacattagt gaacttctta cacgccttac
     2101 attgatagcg ttgagatttg cccctacttt tccccctttt gtaaaattgc ttcggctcat
     2161 tgaatggggt tgattcttct tccgagcagc cttctttatg aaattgataa tctggctcaa
     2221 catcttggat gctattgatt tctataaggc gtttaatttc ttcaacaaca gaccaattag
     2281 ataaaggggt tactgtacag ttttgcgata cacctctgcc aataggattg tcattgcagt
     2341 acataccttt atgaccttta tctttggaac taccagtcat tttatatcga cttggtttcc
     2401 cttttacatc tttgaatttg tgctgttctt tcccaaaatt acaacaaaac ggattgatgc
     2461 agtaattata ttgaatggga tattcagaac cgttccattt aaaagacgtt ggcaagaaca
     2521 acattttgtt ataccttgtt gcaaatcgtt ttggagttaa taaatcatat tgtttagccc
     2581 tatcttttaa ttcttcgttt gaaatcggaa ttttaacatc tacaatttca tcttccttcg
     2641 tggctaatct tttaagcttg gtcattgtct atcaacccac tctatctttg atacccattt
     2701 tttcaacaat ccgcttatta cttcgcttgt tggcaatcca ttcaataaac ttcttatcgt
     2761 ctaaaatgtt caataagaat tcatccactg cttcgctttc agtacgctct gcatattcag
     2821 cgtattgctt aactatcacc cttacttgct ctgatatttc ccaatccaca gacttagcgt
     2881 tcttattttt tggctcaaga aattgcatag gaaaatgcac ccctttttgc ttagtaaatt
     2941 gtaaccattt taacacacca atttgcattc ctacaataat tatttcacgt tcgcattttt
     3001 atatacattt taacttacat tttgtggtgt aaattaacaa caaacgttga tttaacaata
     3061 aaaaacgagt atcaaattca gttgcaattt gatactcatt taggtttaca ttttcacttt
     3121 ttgtactcca aaactgaact acttaaatct atactgctgc ctcttttttt tagtacatgt
     3181 ccttcatctt cgcaggaagt gccaatatga ttttaagaaa ggtaatagac aaaaagtcaa
     3241 aaagataaaa cagagtttct tctttacaaa gggaatgtat gttcgtataa tggtattgag
     3301 gtgaaggtta tggagaaaaa atatggaaca gtagataaga tccgtacttt gcaagttagt
     3361 gccagaccgt tgatccgttt cagtttaaat ggggtcaatt gtttgattgc ttcccatagt
     3421 ttgaacttcc tagctgaagt agacgaaggg atgaagttag tcgttacagg ttattataat
     3481 aatagaaagc aatttgttgt gagatcctat catgtgttag ggaaacccaa aattgtggta
     3541 gaatatgaga aaagtctata cccaagaaaa aaggtccgac aagatgaaag aataaactag
     3601 aaaacaagcg gctgagacaa aaatgtccta gccgcttgtt ttggaaagat ggatgcagac
     3661 gatgggattt gaacccacac gagcatacgc tcacaagaac ctcaatctat atataactac
     3721 atgtatttaa gtatttgtga tgaatcacta aactttccaa actcatttga gggatattgc
     3781 ttaaagattg aacagatcaa ttcgaagccc tattagtatt cgctcaatgg ggggatcaga
     3841 ggccataaaa agagactagt cattactagt ctcaggtagt ggaattgtag gattttaaac
     3901 gttcctggag agtaccggta tgtaactcga agaggtgatt atcatagtcg taaaagtaaa
     3961 ttgaagaagc ctcgcctttc acacgagaac gtcctggttc gacagttaaa cctaacattt
     4021 ggatttttga gacaaacgaa tctatatctt gttcgtcaat tttaaaagca atatgattgt
     4081 atgtctttgg cagtttattc gacgaattct gcattactgc aatccatagg tccttaacta
     4141 agaaaaattt ttctggatat aatgaaaatt tttttgttga ctatcgtaga tttcttttgc
     4201 atcaaataag gtatttaaca attaagtatt taaaagataa cttttaagtt atctttaagt
     4261 aatgcttaaa gctatgttat actctattag gaaatataag aaggagtata aaaatgaaca
     4321 aacataatag aaaaataacg aataacaaag aactgatctt gttactaata gggatttggg
     4381 gtatcttttt actctttaat atgtatatgc cgactatgag agcggatgac gtagtttatg
     4441 caagtagatt agat
//
LOCUS       DAZBSA010000304         3796 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.guided.165, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000304 DAZBSA010000000
VERSION     DAZBSA010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.guided.165"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            110..193
                     /locus_tag="ACHGMT_003029"
     CDS             110..193
                     /locus_tag="ACHGMT_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFN6836673.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            318..1055
                     /gene="erm(B)"
                     /locus_tag="ACHGMT_003030"
     CDS             318..1055
                     /gene="erm(B)"
                     /locus_tag="ACHGMT_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFN6836674.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1318..2181)
                     /locus_tag="ACHGMT_003031"
     CDS             complement(1318..2181)
                     /locus_tag="ACHGMT_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFN6836675.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2183..2455)
                     /locus_tag="ACHGMT_003032"
     CDS             complement(2183..2455)
                     /locus_tag="ACHGMT_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFN6836676.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2472..2681)
                     /locus_tag="ACHGMT_003033"
     CDS             complement(2472..2681)
                     /locus_tag="ACHGMT_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFN6836677.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2759..3655)
                     /locus_tag="ACHGMT_003034"
     CDS             complement(2759..3655)
                     /locus_tag="ACHGMT_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN6836678.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
BASE COUNT         1174 a          662 c          586 g         1374 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccaaa attttgtata ataggaattg
       61 aagttaaatt agatgctaaa aatttgtaat taagaaggag ggattcgtca tgttggtatt
      121 ccaaatgcgt aatgtagata aaacatctac cgttttgaaa cagactaaaa acagtgatta
      181 cgcagataaa taaatacgtt agattaattc ctaccagtga ctaatcttat gactttttaa
      241 acagataact aaaattacaa acaaatcgtt taacttctgt atttatttat agatgtaatc
      301 acttcaggag tgattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg
      361 aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg
      421 aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa
      481 cgtctattga attagacagt catctattca acttatcgtc agaaaaatta aaactgaaca
      541 ctcgtgtcac tttaattcac caagatattc tacagtttca attccctaac aaacagaggt
      601 ataaaattgt tgggaatatt ccttaccatt taagcacaca aattattaaa aaagtggttt
      661 ttgaaagcca tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct
      721 tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc
      781 ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac
      841 ttacccgcca taccacagat gttccagata aatattggaa gctatatacg tactttgttt
      901 caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa
      961 tgaaacacgc caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt
     1021 ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt ttgtaaattt
     1081 ggaaagttac acgttactaa agggaatgta gataaattat taggtatagg gcacctctaa
     1141 taactaccaa ttaattcacc tcaaaatgaa attagaaaaa aacagcgaaa tttttaaatc
     1201 tatttcttat cgatacaaat tcctcgtagg cgctcgggac cccaacatga ggaaatgttt
     1261 ttataaaaaa agagtagtta ccgttttttg gtaactactc ttttcctttt ttaaggttta
     1321 aatacctgga agtttaggtg ttttgggtat tggtggtgta ggtagctgca agctttccat
     1381 tttttgttga attgctttaa attcaggggt ttcttggtgt tgattttgaa ccattttttg
     1441 ctctattctc tccaaagtcg gttgaatttc tttccctgat actttacgat ttaattctct
     1501 ttctaaggtc tctttcggac taatggaagg cgtttctaag cttgaataga gtttcactcc
     1561 ttctctattg taaagcctta tatcgctaaa taagcccgtt ttatgaaggg tttctaaatt
     1621 ggtcggtaag tttttgacaa caatatcatg cgcttgtttt ggtgttgccc tagctgtcat
     1681 tggatcatct gcatacatgg tttcatatcg ttcaatcgtt cctaagtatg attcaatttt
     1741 cggtactgcc atgacgtata tttttgtttc ataaccttta gattgaagca ttgtggctgt
     1801 ttgaatggga acgtctgttg ttcgtcctgt tccttcaatc actaaattat agccttgatc
     1861 gcttaaacgg ctaataagtg cttctgtcat gcgattcgaa taaggggtaa cgtgttttac
     1921 tacgtctttt tcatagagtt tcactaattc gtcaaaattt gggtgctgtt gtttaaaggt
     1981 atcattatca atgacaataa cattcccttg tgtttcttca aaaattgctg atcgcaaact
     2041 ggttttccct gaccctggtt gcccaccaag taaaaaagcg gttggcgatt caaccgctag
     2101 tctattttta gttagtcgtt caacattctt ttctaaacga tcttcaaatt gcttttcggt
     2161 gaagtcagta atattcgcca tgttaagcca ctttctcttt attcaaaatt tgttttgaat
     2221 attgattcat tgaccgccaa tactcatgaa tagcttgtaa ttcttcaaat ggttgttgaa
     2281 ataaatcaac ttcttgtgcc acttctaatt gatttaaaag attcacttct agtaaacgag
     2341 tatttttagt atctagttca tgattcaaaa cataatttaa tacccgatta taaacacttg
     2401 ccgataactc attaatgatt tcaatttcaa atgttttttc atacgtaact gccatttcat
     2461 tttcactcct tttaaagttt gtcaggtaaa tatttccgta tatattcttc taaggcttga
     2521 tccataactt cttttacgtt tccgccattc tttgctgttt cgatttttat gatatggtgc
     2581 aagtcggcac gaacacgaac cgtcttatcc cccattatat ctttttttgc gctgattggc
     2641 gtatcatttc gttttgcttt ctgtgcgcct aaatttccca cgatcgctca cttctttcta
     2701 tttcttctta ctatttattt tatcatcacc tatcacaaat cacaagtgat ttgtgattct
     2761 attctttttc attttctaat tgaatgattc gctcaatcat ttcaaaaaat acgttctcat
     2821 acatagacaa aacttttttg tcatagcctt tgtgttctgt aatgccattt ttagaccaag
     2881 tacttacttt attacttcgc ttgataatat ttcggaaaac caaattatcc tctttatgtt
     2941 ctttgtacag ttcttccagg tttgatttta tcgttgcgct gtccgtatca actaaataag
     3001 gaacaaaacc aatcatatct agtccagggt taaactgttc ttgcaaatca atcaaatagg
     3061 aaatatagtt ttgaatgttg tttgtacttt cttcttctgc ttgtaaaggg atcataacgt
     3121 aatcactcgc cacgattgca ttatttgtat aaacgcttgg cgttggtaca gtatcaataa
     3181 taataagatc atagtcactt tttaaaggtg ctaaaagagt agcaagcaat ctactttcat
     3241 tttcaaacgt ccatgagcga gttaattttg gcagtaacat caaatcaaac gtgccaggga
     3301 tcaagtctaa attatcagtc aaatgaataa tagaagaagc caagtttcca tttttcaagc
     3361 cttcataaaa attgacacgt ggcaattcta cctcaaaagt ttttgctaag tcttttgtca
     3421 atgttgcctg taagtcctta tcgatcatta aaacttttaa attcaatttg tctgtcaagt
     3481 aagcaaacat agtcgataat ttggactttc caacaccacc tttaaagtaa ttattcaaga
     3541 taataattgc ttcattttta ttgtttaaaa tacgtcttaa ttcttcaagt atttttagtt
     3601 tttccttctc cataactacg ccccattctt tttttgtata gtaatattgt atcattaaga
     3661 atcacaaatc acaagtgatt tgtgattaat cacttgtgat ttgtgataag tgatttgtga
     3721 ttaatatata aaagccctct ttaaagggct tttataggtt ctgttgcaaa gttttaaata
     3781 aagaataaaa tccctt
//
LOCUS       DAZBSA010000305         3312 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.guided.174, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000305 DAZBSA010000000
VERSION     DAZBSA010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.guided.174"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            118..303
                     /locus_tag="ACHGMT_003035"
     CDS             118..303
                     /locus_tag="ACHGMT_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFN6836679.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            461..628
                     /locus_tag="ACHGMT_003036"
     CDS             461..628
                     /locus_tag="ACHGMT_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN6836680.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            1246..1905
                     /gene="vanR-B"
                     /locus_tag="ACHGMT_003037"
     CDS             1246..1905
                     /gene="vanR-B"
                     /locus_tag="ACHGMT_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFN6836681.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            1908..3251
                     /gene="vanS-B"
                     /locus_tag="ACHGMT_003038"
     CDS             1908..3251
                     /gene="vanS-B"
                     /locus_tag="ACHGMT_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFN6836682.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
BASE COUNT          981 a          640 c          833 g          858 t
ORIGIN      
        1 ttcatactct gcccgtggaa tgttggtata tgcacctttc ttaaagttta tatttcacat
       61 tttgtttctt ttattcttcg agcaaaagaa ttataatgta ccctgtggag gtgtactatg
      121 gactacatga cgttgaaaga ggcaggcgag aaatggggtg tgcccccacg atggataaac
      181 tactattgtg ctgcgggacg catccccggc gctgtaaaga tggcgacgat ctggctcatt
      241 cccaaaactg cggaaaagcc gattgacagg cgtacaaaga aaggaaaggc ggttactgaa
      301 tagtataatg aaaccggcgc attttggata tggttttgaa gagagagaaa gcatggtatt
      361 ttgtcacgga aaaactataa gatgatagat ggcaggctgc tccagaccga taaaaagtat
      421 tctcatctga aacagaaaga aaagatttac acatggatgt atgaagaagc gaaactttat
      481 catgacagaa atggaaaatg cccgaaacaa agacgaggaa gctgctgctg tatcagccgt
      541 atatggtaga attgaaaagg ccggcatctg gattctttat ggtgaagtct ccagacattg
      601 gataactgct ggtctaatgc gcctttgagt atggggtgat tgattttaat accggggagg
      661 tacggctttt tgaggaaaat attctgggat ttacatttgg cgattattac atttcctgta
      721 actgcaagga cacggatgat gaagatatga ggaatataga cttgctgatt ttctactgtc
      781 ttgaacggta ttaggactga atctgctgcg cggagtgcgg caagagcgac cggagggagt
      841 acacacaggc cctgcctgtg tgaaaagcca taccgcaagg tgcattcgca aaaaagaggc
      901 cgcgcatttt gctgaggctt ggggtgtccc caacaagcaa aacgagaacg ccgcaggcgg
      961 gcaagtggtg gatcagaata tccgtaaagg atattctgat ccattgcttg ccagtattat
     1021 cgggatgttt tgacaaccgc tgtaatcgaa acatcccaaa cattaagaag gtgttaatta
     1081 ttattatctt gatttaattc gtttaaacca ataatataga tacatacacc atgaaaagaa
     1141 gcgagtatcc caatgacgga tgattaaaat ccgaaaagaa atcgctttga aactacaggg
     1201 aaactacaga ctgttatgtt atcttcttaa aatggaggga tttttatgtc gatacgaatt
     1261 ctacttgtcg aggatgatga tcatatctgt aatacagtaa gggcgtttct ggctgaggca
     1321 ggatatcagg tggatgcctg cacagatgga aatgaggcat acaccaagtt ttacgaaaac
     1381 acttatcaac tggttattct tgatattatg ctgcccggta tgaacgggca tgaacttttg
     1441 cgtgaatttc gtgcgaaaaa tgatactccc attctgatga tgacagccct gtcggatgac
     1501 gaaaaccaaa tccgggcgtt tgatgcagag gcagacgact atgtaacaaa gccattcaag
     1561 atgcagattt tactaaagcg ggtggaagcc ctgctgcggc gcagcggtgc gctggcaaag
     1621 gaaatccgtg tcggcaggct gacacttctg ccggaggatt tcacggtact ttgtgacggt
     1681 acagagctgc ccctgacacg aaaagaattt gaaatccttt tgctgctggt gcagaacaaa
     1741 ggcagaacct taacgcatga aatcattttg tcacgtatat ggggatatga ctttgagggt
     1801 gatggcagca cagtccacac tcatatcaaa aatttgcggg ccaagctgcc ggaaaatatc
     1861 atcaaaacca ttcgcggtgt aggataccga ttggaggaat cataataatg gaaagaaaag
     1921 ggattttcat taaggttttt tcctatacga tcattgtcct gctactgctt gtcggtgtaa
     1981 cagcaacact gtttgcacag caatttgtgt cttatttcag agtgatggaa ttacagcaaa
     2041 cagtaaaatc ctatcagcca ttggtggaac tgattcagaa tagcgatagg cttgatattc
     2101 aagaggtggc agggctgttt cactacaata accaatcctt tgagttttat attgaagata
     2161 aagagggaag cgtactctat gccacaccaa atgccaatac atcaaatagt tttagaccca
     2221 actttcttta tgtggtacat agagatgata atatttcgat tgttgctcaa agcaaggcag
     2281 gtgtgggatt gctttatcaa gggctgacaa ttcggggaat tgttatgatt gcgataatgg
     2341 ttgtattcag ccttttatgc gcgtatatct ttgcgcggca aatgacaacg ccgatcaaag
     2401 ccttagcgga cagtgcgaat aaaatggcaa acctgaaaga tgtaccgccg ccgctggagc
     2461 gaaaggatga gcttggcgca ctggctcatg atatgcattc catgtatgtc aggctgaaag
     2521 aaaccatcgc aaggctggag gatgaaatcg caagggaaca tgagttggag gaaacacagc
     2581 gatatttctt tgcggcagct tctcatgagc taaaaacgcc catcgcggct acaagcgttc
     2641 tgttggaggg aatgcttgaa aatatcggtg actacaaaga tcattctaag tatctgcgcg
     2701 aatgcatcaa aatgatggat aggcagggca aaatcatttc cgaaatactg gagcttgtca
     2761 gcctgaatga tgggagaatc gtacccatag ctgaaccgtt ggacataggg cgcacggttg
     2821 ccgagttgct gcccgatttt caaaccttgg cagaggcaaa caaccagcgg ttcgtcacag
     2881 atattccagc cgggcaaatt gtcctgtccg atccgaggct gctccaaaag gcactatcca
     2941 atgtcatatt gaatgcagtt cagaacacgc cgcagggagg cgaggtacgg atatggagtg
     3001 agcctggtgc tgaaaaatgc cgcctttttg ttttgaacat gggcgttcac attgatgata
     3061 ctgcgcttcc aaggctgttc accccattct atcgcattga tcaggcgcga agcagaaaaa
     3121 gtgggcgaag cggtttagga cttgccatcg tacaaaaaac gctggatgcc atgagcctcc
     3181 agtatgcgct ggaaaacacc tcggatggcg ttttgttctg gctggattta ccgctcacat
     3241 caacattgta aatatttaaa acttaaatga ttttgggctc tttgtcaaat aggactgata
     3301 gagtgagata ag
//
LOCUS       DAZBSA010000306         2362 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.guided.195, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000306 DAZBSA010000000
VERSION     DAZBSA010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.guided.195"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..577
                     /locus_tag="ACHGMT_003039"
     CDS             <1..577
                     /locus_tag="ACHGMT_003039"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN6836683.1"
                     /translation="MFCEKGYDNTSLQDIINETKLSKGAIYHHFDSKEDILKAIFQRI
                     GNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSVLLTVSPQLLNNPRFLAMQIE
                     QMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMVLSNVWLNPLVEMTDKEGMEK
                     RCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            684..1922
                     /gene="mef(H)"
                     /locus_tag="ACHGMT_003040"
                     /gene_synonym="mefH"
     CDS             684..1922
                     /gene="mef(H)"
                     /locus_tag="ACHGMT_003040"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFN6836684.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            2149..>2362
                     /locus_tag="ACHGMT_003041"
     CDS             2149..>2362
                     /locus_tag="ACHGMT_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016438386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HFN6836685.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLH"
BASE COUNT          768 a          326 c          417 g          851 t
ORIGIN      
        1 aatgttttgt gaaaaaggat atgataacac atcattgcaa gatattatca atgaaacaaa
       61 actttctaaa ggtgctattt atcatcattt cgattcaaag gaagatattt taaaagcaat
      121 ttttcaaaga attggaaatg aaaatgcagt aatatttgct aaaattagag ataacgatag
      181 tttaaacggt ttagaaaagt taagagaaat atttaagaca gcggtattca attctaacca
      241 aagcgtttta ttgacagttt ctcctcaact attaaacaat cctcgttttc ttgctatgca
      301 aatagaacaa atgtatgaaa ttgtagtacc tgaatttgtt gaacccgttt tagaacaagg
      361 tatcaaggat aagtctttgg agattgaaaa ttcacatgaa attgcagaag ttatcatggt
      421 attaagtaat gtatggctca acccattagt tgaaatgacg gataaagagg gtatggaaaa
      481 acgttgcaat acattcaatg cccttttaaa tggaataggc atagacacat tattagacga
      541 agaaacaatt tctgcattta tacagttttg taaataactg tatttgttat ccctatacga
      601 tagactgctc tataaaagag cagtttattt aaaccattat ataaaccgac ggtctgtttg
      661 attaaataaa aaggagagat tttatgcaag cagaaaaaaa actttttaat cgtgatttta
      721 tacttgttgt tataggacag ataatttctt tatttggaaa cgctatccta cgatttgcat
      781 taccgtacta tttgttaaaa gaaactggtt catcagcttt gtttggtatt gctacagcct
      841 gctctttttt acctatggtt attttgtcgc tggtaggtgg tattattgct gacaggacaa
      901 ataaaagaaa cattatggtc atactagatt ttgtaacagc aatcataatt ttagtatttt
      961 atttgtcatt gggaaaaatt cctatcatac ctttgtttat tgttgctttg atgttacttt
     1021 atggtatttc tggtgcatat caaccagcgg tacaagcaag tattcctcta ttggtatcat
     1081 cagataaact taccgtcggt aatgctataa taaatcaagt aaatacttta gcaaacttta
     1141 tcggaccagc tataagcgga gtagtttttc aattttatgg tatcaatccc attttaattg
     1201 ttagtattat atgttttact ttttcagcca ttatggaaat ttttatccat attccacacc
     1261 aaaaaagacc tcgtgaagta ggtgtaattg cagttgctaa aaacgattta aaagatagtt
     1321 ataccttttt gaaaaaggag aaacccattt ttatatccgt aatctttttg gtttgcattt
     1381 tcaatttggt tttaagtgct gttttaattg ttggtctgcc tatcttaatt tctgaagttt
     1441 taaaaatgcc agatacacta tatggttttt cacaatcagc attatcatta ggtggtttat
     1501 gtggtggtat tttaacagct cttgttgctt caaagttaaa actaaaaaac ctttatacct
     1561 tgttacttat ttgttctgtt tctgttttac ttatgggaat ttcgctattt ttagaattgc
     1621 ctgcaatagt ttcctactgg gtagttacag tgatgtgctt tgtggctatg ggtgtatcta
     1681 ctatgtttgt tgttcaaatt tacactcttg ttcagacaca aacaccaccg caattagtag
     1741 gaaagataat ggctacgctg gtagcagttg ctatgtgcgg tcaacctatt ggacaaatgg
     1801 tttacggtat tttattcgat gtatttatac aagaaacatg ggttgttatg tttgtagctt
     1861 ctattgcttc aatcatgatt tcaatatatt caaaaagaat ttttcaaaag ttagagatat
     1921 aatacaaggg gttaaatagt atgttaggct aaaaacaata tataaaagaa aatttgaaat
     1981 ctgctaaaag gatagtgtta taaaagaaag agtaatattg cagtatttaa ggctcgcaca
     2041 aatatatgtt ttgtgtgggc ttttttctta ccatttttct gtatttctaa agggtataaa
     2101 cggtattccg tttatatatt tcagcattag cagaaagggg gtgagattat gaggacatct
     2161 tctttcgagc atatcgtcag attacagttc aattctttaa tgctgattgt cattaagaac
     2221 aaagtgaaaa gtcgaaacag acagcttgca agacattcta aacgtgaagt attgttttgt
     2281 gaaatagccg aaacgaaaca agttgaacgt ggaacgattg atacctgttt ttatgattgt
     2341 gtatcattta aagtcttgca ct
//
LOCUS       DAZBSA010000307          918 bp    DNA     linear   BCT 21-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7513
            SAMEA115709100-rid22983843.guided.260, whole genome shotgun
            sequence.
ACCESSION   DAZBSA010000307 DAZBSA010000000
VERSION     DAZBSA010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709100
            Sequence Read Archive: ERR13198818
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002337979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002337979.1
            Genome Coverage       :: 99x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/21/2024 12:44:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,042
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,904
            CDSs (with protein)               :: 2,904
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 36 of 82
            Pseudo Genes (internal stop)      :: 26 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709100-rid22983843.guided.260"
                     /isolate="VRE7513"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            305..853
                     /gene="aac(6')"
                     /locus_tag="ACHGMT_003042"
     CDS             305..853
                     /gene="aac(6')"
                     /locus_tag="ACHGMT_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFN6836686.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          333 a          130 c          187 g          268 t
ORIGIN      
        1 taaaaattct aaaaattaag attattatat gaaaacaggt tttatttgtc aattcataat
       61 tggttttttt taaagcaaaa gataatctat atcatgatga acaaacagag ttttttcctc
      121 caattgatgg ctttcctgat ctttcctatt ttgaataaag tgtttctgta taatttttta
      181 ttttataagt aagataaata atgaaagaat agagatactg attggtagct aaagattttt
      241 gagcgagaac atttcgtttt ttcaaaagga ctgttagaat aagcaagaac aagggggaag
      301 acgtatgata atcagtgaat ttgaccgtaa taatccagta ttgaaagatc agctttctga
      361 tttactgaga ctgacttggc cggaagaata tggagacagc tcggcagaag aagtagaaga
      421 aatgatgaat ccagaacgaa tcgcggtagc agcggtagac caagatgagt tagtaggatt
      481 tattggtgca atccctcaat acggtatcac aggttgggaa ttgcatccat tagttgtaga
      541 aagctcccga cgaaagaacc aaataggtac tcgattagtc aattacttag aaaaagaagt
      601 agcttccaga ggaggaatca cgatttattt aggtacggat gatttagacc atggaacaac
      661 gttaagtcaa acggacctgt atgagcatac atttgataaa gtggcttcca tccagaacct
      721 tcgtgaacat ccgtatgaat tctatgaaaa attaggttat aaaatcgtag gtgtcttacc
      781 aaatgcaaat ggctgggaca aaccggatat ttggatggca aaaacgatta ttcctcgacc
      841 aaattctcaa taataaaaag aggttgtgac agaagtaatc agcttcaaga aataagaagg
      901 aatttacgaa aattactt
//
